LOCUS       DAOSOL010000001        19382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_2824_length_19382_cov_4.009986, whole genome shotgun sequence.
ACCESSION   DAOSOL010000001 DAOSOL010000000
VERSION     DAOSOL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 19382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19382
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_19382_cov_4.009986"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK171_00005"
     CDS             complement(<1..480)
                     /locus_tag="PK171_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT04617.1"
                     /translation="MNILVVNCGSSTVKYELLSMNDNTDNQVIAKGLVERIGIPGSRL
                     EYKTNHQVSYSKEREVPNHRIALEWIIEVMTDPEIGVIKDPSEIDAVGHRVVHGGEKF
                     TASILIDNEVLKTIRACSELAPLHNPPNISGIEACQALMQKAPQIAVFDTAFHGTIPE
                     "
     gene            complement(598..1593)
                     /gene="pta"
                     /locus_tag="PK171_00010"
     CDS             complement(598..1593)
                     /gene="pta"
                     /locus_tag="PK171_00010"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOT04618.1"
                     /translation="MNVLEEIRNRAKSLKKTIALPEYDDERVLQAAEIATRDQVAAIQ
                     LIGDPAIIEQKTKSLGISLTGVEIIDHKKDQQRAEYVRSLYELRKGKGLTPEQAEQWL
                     DSSMYYACMMLYHDRIDGIVSGAALSSPDVIRPALQIIKTAPGVKLASSCFLMVVPDC
                     LYGSNGVFLYADSGFNPNPDSEELAHIAITTARTATQLLDVEPIVAMLSFSTKGSARH
                     ELVDKVIEATAIAHSLKPELLIDGELQGDAALVSSVAEKKAPGSKVAGKANVLIFPDL
                     NAGNICYKLTERLARATAIGPILQGLAKPVNDLSRGCKAQDIVDQIAVTVLQTQF"
     gene            complement(1747..2223)
                     /gene="coaD"
                     /locus_tag="PK171_00015"
     CDS             complement(1747..2223)
                     /gene="coaD"
                     /locus_tag="PK171_00015"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOT04619.1"
                     /translation="MIAIYPGSFDPVTNGHLDIIIRGSKIFDGLIVGLLKNPNKKPLF
                     TVEEREAMMVEAIKDLNNVTVEVFQGLLVQFARQKNCRIILRGLRAISDYEYETQIAI
                     TNRKICPEIETFLLPTSTEYSYLNSTVVKEIAQFGGCIRGLVPEGVEKKLRKKFQA"
     gene            complement(2285..2839)
                     /locus_tag="PK171_00020"
     CDS             complement(2285..2839)
                     /locus_tag="PK171_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOT04620.1"
                     /translation="MGYLYITGGSIRNRKIIGPQNQKTRPMQAFLRKTLFDLMPLSLE
                     KSIIWDIFAGSGAIGLEALSRGAGKVFFVEKDIKMTTVIGRNVKLCGFQEQSQVLLAD
                     FFDLKRWVSRVEPPEIVFLDPPFPINPVEVLEKMYNLFELVKNSLIVIRFSKKSNPSL
                     ECPFYRVIIRRAYGDSALLFGSFE"
     gene            complement(2992..5340)
                     /gene="recG"
                     /locus_tag="PK171_00025"
     CDS             complement(2992..5340)
                     /gene="recG"
                     /locus_tag="PK171_00025"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT04621.1"
                     /translation="MEKDRDRLKKIIEQESQRLDNQTVFGGFNQYVKKMIQSLSIPVP
                     EEIFLSVNRYPQMSKPARSIFLKNLETCIIDGHQPQPSASQPSSPIKPVDFQLSVRTI
                     KGVGPVVEKQLNRLNIYTREDLILYFPRHYQDRGQIIPIAKAEGSGLQTVLGKVVRHD
                     RISTRRGRLLKVVIQDQTGQISLVCFNRDFLARILTPGKKVLITGFFQTAYGKKETSR
                     FEYEIIDSEDIQYESILPVYGLTDGLNAKKLRSLIDIALKTSLAAFQDTIPKPIQEKY
                     SLLRKPGAILELHHPSRSSITELNKHRSRSQVTLIFEEFLLFALSLKVRRHQIQALQV
                     QPIVPDFTFINRFIQQLPFELTLAQKKVLKQIENDLTSGKIMNRLLQGDVGSGKTLVA
                     IINALYIIKAGQQVAMMAPTEILADQHYQRWSQILKEFGIEVSLITGGQSKEKSEIIE
                     KIGEGRVHFVIGTHALLEDYVQFNRLGLVIVDEQHRFGVKQRSRLKEKAVVPHLLVMS
                     ATPIPRTLALTLYGDLDVSVLDGKPIGRKPVETVAFSMKEQKQAFQRVGSFLEKGQQA
                     FVVCPAIEESDLELSNVYEVYEQLQKKWFSHSQIELIHGKLPSKEKDDIMGRFSRGEI
                     QVLVATSVIEVGIDVPNANVMVIENAERFGLAQLHQLRGRIGRGDQAGWCVLLYQGKN
                     QDIQKRMEIMVNTDDGFQIAEEDLKMRGPGEFYGVRQSGVLEFHLADPFRDWKILEKA
                     HQEADLILQNDPRLQKPENQILKKEIERRFFQFLPEELMIEA"
     gene            5470..5679
                     /gene="rpmB"
                     /locus_tag="PK171_00030"
     CDS             5470..5679
                     /gene="rpmB"
                     /locus_tag="PK171_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOT04622.1"
                     /translation="MTRKVGFKMAICEVCGKKPVFGNKISHSHRVTRKMWRPNIQKVR
                     VRLNGHSAKMNICTSCLKAGKIKKA"
     gene            complement(5735..6400)
                     /gene="rpe"
                     /locus_tag="PK171_00035"
     CDS             complement(5735..6400)
                     /gene="rpe"
                     /locus_tag="PK171_00035"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT04623.1"
                     /translation="MAILAPSILSCDFSRLKDQLLMAQEGGVEMIHIDVMDGHFVPNI
                     TFGTLIVDAVRKILPKAFLDVHLMIQNPQDCYLDFINAGADNISFHLEVVPDFDFLIN
                     LIQKAGAKASLAVAPGTPVHMLDQILPKLYLVLLMTVNPGFGGQKIIPGMDQKIIQLR
                     KKIKSKGLNTLIEIDGGIHENNIEYLASCGASVIAAGSLVFSDENKITQKVREISDKI
                     KRF"
     gene            complement(6423..7460)
                     /locus_tag="PK171_00040"
     CDS             complement(6423..7460)
                     /locus_tag="PK171_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOT04624.1"
                     /translation="MTAKRVKTPNLFLRILLTVIAFGLGVVLSGFFGLMLLQYSVNAT
                     SLSVPDFRNQDLVSAVNLASKMGLQVEVARVESRSDRMANQVLEQDPLPGADAKRGRK
                     IRVVVSGQTGQAQAGGAVVAPTAAQEVKMSRVPDVRDLSLNEAQNLLETNGYQLGRVV
                     EVTHEDVLKGMVISQDPPAESTLPLGSQVSLLISKGGTAAIAATPPAKILVPDLIGLK
                     VEEARRLLAEMDLSVGQIEEVAMPERNPGIIIGQMPESGQEASVGQAINFQVVQAIQD
                     LQDLSLRFALPDARVPITVRIVVNDELGERVVYEEQHQGGETVEILSRTKGKGKVLIY
                     LNGYYYWEKEL"
     gene            complement(7457..8815)
                     /locus_tag="PK171_00045"
     CDS             complement(7457..8815)
                     /locus_tag="PK171_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT04625.1"
                     /translation="MKGIIFIKVNVRLEAFRILNRLFRGEQVFLDKEYSASIKTGDLS
                     IPDRSFLVHLVRGIVKFYPQLQEIASTYLQKPKSLPKKVQIILYLGLFQLAVDTRIPD
                     YAVVSETVELARLTHSHPWIKLVNAISRKAAAERIRWCKQAEHWSMVLPQWLEEYWRI
                     YLTRGEMNQLKKSLVLPPVLYLRVNSLKTNSQKLIETLSKKYSIVVNSLPMLPNTLNT
                     QLDYSQLVETDEYQKGYFSIHDFSSQIMVHLFAPKPGEKIVDIGCGSGGKTLMMAQIM
                     KNQGCIHAVDVHPEKLKTLEVLAHRQGIDIIQTLAADATQPLPQELFSSDRVFVDAPC
                     SGWGTLRRNPEIGILRNKSDNQSLAEKQFQLLCQASALIKKNGIILYCVCTMTPEETE
                     GVVGAFEKLKPEEWEVNLPGDSQLQPELQSTILLTDDKSYRIWPHYFNSDGFFGKAWR
                     KL"
     gene            complement(9009..9689)
                     /locus_tag="PK171_00050"
     CDS             complement(9009..9689)
                     /locus_tag="PK171_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOT04626.1"
                     /translation="MFYPFDYTFILLIPALILAFYAQSKVKSTYATLSQKRSRAGLSG
                     FEVAQQLIQQNNLSLRVEETPGQLTDHYDPSREILRLSTGVARGGSIADYSIAAHEVG
                     HALQKREKYGAFALRSILVPVASIGSQAAFPLFFIGLIFSFRFLMDIGIIFFSLAVLF
                     QVVTLPVEYDASRRAYAALTNSGIVAVDEQPDIKKMLNAAALTYVAATAMAALQLLRL
                     ILLRQSRD"
     gene            complement(9704..10639)
                     /gene="fmt"
                     /locus_tag="PK171_00055"
     CDS             complement(9704..10639)
                     /gene="fmt"
                     /locus_tag="PK171_00055"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT04627.1"
                     /translation="MIKVVFLGTTPLSIGVLSALVDAQYNIAAVITQPDRECGRGRKL
                     TAPPVKVWSQDQGFKVLQPDKVNRKDFLDEINKIAPDIMIVAAYGQILRSSLLEIAPF
                     GCINVHASMLPRYRGADPIRWVILNGEKKTGVSIMLMDEGIDTGPVLATREVMIEDSE
                     NSITLENKLGKIGGELLVEVLPAWIEGKIKPIPQSNEQASYAGKIPKDLYQIKWNRSA
                     EEIVRQVKAFAPCPGAFGYFHQKRLKVIKAHVYKQKEKVKAGQIIGYEPKVGLLVGTG
                     KGILAIEVLHPENRKVQTGQEFCCGYRIQPGDLFQ"
     gene            complement(10636..11145)
                     /gene="def"
                     /locus_tag="PK171_00060"
     CDS             complement(10636..11145)
                     /gene="def"
                     /locus_tag="PK171_00060"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT04628.1"
                     /translation="MADLNIEKYGSQILRQKAEPVQNIDGKVKKIIQDMFETLHKNSG
                     LGLAANQVGILQRLVVLTNPNTNQDMAFINPEWKEVDPEKEIGEEGCLSVPGIFSKVN
                     RFQKIQVTTQDVQGRQLDFFADGLLARIIQHEIDHLNGILFIDRLSPTRRLVLANKLS
                     RINTNGDHE"
     gene            complement(11357..13438)
                     /locus_tag="PK171_00065"
     CDS             complement(11357..13438)
                     /locus_tag="PK171_00065"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04629.1"
                     /translation="MKMNSVSVALPLPVYKVFSYRIPQEMSDKIQIGTIVEVEFHGSR
                     RFGCVWSFDSVQNDKEYELKSLLSCLPLEPLADWQMEFAKRISNQTFSSLGEALALFV
                     PKYQLSWEKWTSQRIFLINEDDEYFLDLKKHGVDPISQGVNLFIFKDLLGLTTQKINQ
                     LIKKNRIKVVEEDYKKNQYSLPPQKWNKKWWQRGTIAESLNLLGDLIKDSFDRGLKIF
                     ILFPGPKILDYFMENLKTNYPLARFLSFDGRLTARKRMLAYQMVREGYFDVLLGTRLA
                     QFLPIHQIGVQVLFDPDDFGHYSDQQPHYHSFHSLIEKVSITGGDLHIIGTIPDLTLY
                     FGLQEGYFETQKGQVKQFTKEQKLELVTYEQKKTILPQLVQKAIAQNITNRQNSIIWV
                     QKTGYSVALGCSDCGFYYSCSDCDVALRFYRKERILRCPRCGKKVIPESFCPECHGPW
                     MKTWGDGIEKVYQFLKRIFSGVPIVKIASDQERSELDVKGEEPLIIVGTSAALHEELL
                     AHSTLFIIHSFEDWLFLPEFQVREKFYQNIQRALYFLGAAKPPKNRVLIETSKQYQKY
                     IDPFFLPPAEFYKQELEKRKKYGYPPSQGLLQVIARSRNKLNREMVLNKVKNELKVGQ
                     FIVDGPFPGDSYLKKSGWSDQLVIKFEPSVLSTLYNKVTAVVQARKSTGVEVDFKVYN
                     TLPGWVKDDPR"
     gene            complement(13435..14652)
                     /gene="coaBC"
                     /locus_tag="PK171_00070"
     CDS             complement(13435..14652)
                     /gene="coaBC"
                     /locus_tag="PK171_00070"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOT04630.1"
                     /translation="MNIYYPPLFWKNKKILFGITGGISAYKVAGFISHLVQAGSDIQV
                     IMTEAAKKFVGENTFNALTRRPVYSSLFESGEKILHIHLMREYDYLVVAPATANIIGK
                     MAGGIGDDLLSSCFLVDPKKIILVPAMNVDMWGNPIVQENLKKLIAIGVRVMNPSSGS
                     LACGETGAGRLPDQEEFIENLYYFLSSYRSLQGKRALITAGPTREYIDPIRYLTNSSS
                     GLMGCSLAAAARAHGAEVTLIGGPMSVRIPSGVDYLPIVSTQDLETATLKQFDKMDIC
                     IMTAAVADYRPSITSPIKIKKQGKSEIALSLTQNPDILSELGQKKAAQLLVGFCAEDG
                     DIVSEARRKLQQKNCDVMIANRILPGESGFEVNKNKAWLLNKNGALIDIPLMDKMELA
                     ETIVNYLVSLYFT"
     gene            complement(14649..14843)
                     /gene="rpoZ"
                     /locus_tag="PK171_00075"
     CDS             complement(14649..14843)
                     /gene="rpoZ"
                     /locus_tag="PK171_00075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT04631.1"
                     /translation="MLSIDELIKKTGNRYYLTTLVAKRSKQLNQGAKPLIENQQPAKP
                     LFIALKEVAEDKIKWSKETA"
     gene            complement(14968..15570)
                     /gene="gmk"
                     /locus_tag="PK171_00080"
     CDS             complement(14968..15570)
                     /gene="gmk"
                     /locus_tag="PK171_00080"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT04632.1"
                     /translation="MPGLLFVLSGPSGAGKSTVRKAVMEQCEGLKYSISYTTRLRREG
                     EREAIDYYFVDDETFQHMKENHLFIEWAKVHGNFYGTPRLKMEEWLQRGFDVILEIDV
                     QGALQVKRNYPQGIFVFIAPPSLETLGERLRNRKTDREDVIYLRMMNALIEMKSIQDY
                     DYLIINNVLKETVHKFHSVIIAERCRIRYGDDETQELLSE"
     gene            complement(15580..16452)
                     /locus_tag="PK171_00085"
     CDS             complement(15580..16452)
                     /locus_tag="PK171_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT04633.1"
                     /translation="MKSMTGFGHAEGEGTIGFYRISIKSFNHRFSDINLRLPRELCVW
                     EENLISYLKERVSRGKVELKLNFEPSEEAFSVEANTLLAKAYLKALHKLSSELNIPYE
                     ADLRDLMQVPEIIKLKEDDHRWLDEYQRFFPIFKEAVEAFLQYRLREGEKLQHDLIQQ
                     MEKFRELISMVEEKSKNIPEYYREKLNQRLKEIISSVPVNENLLAQEIVFYVDRSDIH
                     EEIIRLKAHICRFDDILSRQDSIGRELEFVLQEMNREVNTIGSKTSNVEISSLIIDLK
                     TVLEKMREQIQNVE"
     gene            16768..18486
                     /locus_tag="PK171_00090"
     CDS             16768..18486
                     /locus_tag="PK171_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOT04634.1"
                     /translation="MRILEGIPLRFQIETVSPLVEGARVQKITLTENRETCLEIRSRN
                     QPGFLVLSTHPEICFFYHSQQKPIMKKIKESAWVQILNKYLVGAKIIGFEQMGWDRVI
                     RLTFQNQKIWVEKNLFYLYCELTGRNANCILTTADDPSYILGCQKIVGPQQNRFRTIV
                     VGKPYQLPPLKINAIDPLLLVQEKAEFPIPKQDDDLPKWLLKNIDGAGPFLATIIAEC
                     LVNSRFNAGNLQLLLSTCLQNIFRPLFNQNLVFSVYISPKDNFPLGIFWLSTQHYAQL
                     PHHDFPNLNEAVKYFAQTYWSLQAFLSQKNKKKSFIEKELLFIKEELLKVGQSLVSED
                     QLVELLTRGNLLKLYPQLNIIKKQPKGFLVANLLTGNSGEVFIEIDPLLSINQNMQLY
                     FRKYRKSKTRNQILKKKKEELEFRKKILEKELINLDQAPLHQEQDDNNSTQSGFFEPG
                     IIKFLTPSKNLILVGKNEKANHLLVTRFSSRDDYWLHVRDFPGSHVLLRISNQPERLT
                     EDLFLAAQIAGYYSSLRNETSVIVMYTPIKNVKTLPHAGLGKMIFRNEKNLTVIPAIP
                     DNLHRI"
     gene            complement(18442..>19382)
                     /locus_tag="PK171_00095"
     CDS             complement(18442..>19382)
                     /locus_tag="PK171_00095"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT04635.1"
                     /translation="KQKIKVYDNEFCRIDIWLHRNFPDFSRSAFSRLIKQGLIKKNQE
                     VIYKPSSEVRPGDILEITWPVDARHTLEPQNLPLDIIYQDDHIVVINKRAGIIVHPVR
                     PFQKDTLINSLLFHGIELSRYGAPLRPGIVHRLDRDTSGVLVIAKTDRAYLELIQMFK
                     NRTVEKFYLALIEGQWKGIKQVDLAIRRNPRVPCLMKISSSGGKPALTEIEPLWIGDS
                     YSLLLVKPRTGRTHQIRVHLSSQGYPIVGDKSYGSNRSGEIMKRQALHAFLIAFSHPV
                     TKTKMFFAAALPSDFQEAINKISNSMKVIGNSRNYG"
BASE COUNT         5840 a         4178 c         3420 g         5944 t
ORIGIN      
        1 ttcaggtatc gttccatgaa aagccgtatc aaatactgct atctgtggag ctttttgcat
       61 aagcgcttga caggcttcaa taccagaaat atttggtggg ttatggaggg gcgctaactc
      121 tgaacaggct cgaatggttt tcagaacttc attatcaata agaattgaag cagtaaactt
      181 ttctccccca tggacaaccc gatgaccaac ggcatcaatt tctgatgggt ctttgataac
      241 cccaatttct ggatcggtca tcacctcgat aatccattcc aatgcaatac ggtgattagg
      301 aacctccctc tctttggaat aggacacttg atggttggtt ttatattcaa ggcgagaacc
      361 aggaatgcca atcctctcta ctaagccttt ggcaataact tgattatcgg tattatcatt
      421 catgctgagc aattcatatt taaccgtcga acttccacaa tttaccacca aaatattcaa
      481 tttccatcat cccttttctt cgaattattt tccttcaaat ttattccaaa gatgaatttt
      541 aattaataac tgtctttccc cttgcataat tgaaaaacct tctttgaaaa aaatcaccta
      601 aaattgtgtt tgtaaaacag ttacggcaat ttgatcaacg atgtcttgag ctttgcatcc
      661 ccgtgacaag tcattaaccg gttttgccaa tccttgcaat attggtccaa tggcagttgc
      721 tcgagccaaa cgctcggtta atttataaca aatatttcca gcattgagat cgggaaaaat
      781 gagaacattc gcttttcctg ccaccttgct tccaggagct ttcttctcgg ccaccgagga
      841 aaccaaagca gcatctcctt gaagctcacc atcgataagc aattctggtt ttaaagaatg
      901 agcaattgct gtcgcttcga taaccttatc aactaattca tgccgggcac ttcctttagt
      961 agaaaaagaa agcatagcaa ctattggttc aacatctaag agctgagttg cagtacgggc
     1021 agtagtgatg gcaatatggg cgagttcttc cgagtcaggg ttgggattga atcccgaatc
     1081 agcgtaaaga aatactccgt ttgatccata aagacaatca ggaacaacca ttaggaaaca
     1141 gcttgacgcc aatttaacac caggagctgt tttaataatc tgcaaagccg ggcggatcac
     1201 atcagggctg gaaagtgctg ctcctgatac aatcccatcg atgcgatcat ggtacagcat
     1261 catacaagca taatacatac tgctatccag ccattgctca gcctgctctg gggtaagacc
     1321 ctttccctta cgcaattcat aaagtgatcg aacatattca gccctttgtt ggtctttttt
     1381 atgatcgatt atttcaactc cagtaagaga aattcccaaa cttttggttt tttgctcgat
     1441 aatggctgga tcgccgatca actgaatagc agcgacttga tctcgggttg caatctcagc
     1501 cgcttgaaga actctttcgt cgtcatattc gggaagcgct atggtttttt taagactttt
     1561 cgcacggtta cggatttctt ccaaaacatt cattccattc ttatctccct tctcaatccc
     1621 ccattgggtt tattattttt aatcactgaa caaaaacatc aactctttat caatagaata
     1681 aagggcaagc cttttagttt atcataaaag ctcagattct ctaaggttct aataaaacaa
     1741 cctgccctaa gcttggaatt tctttcttag ttttttttcc acaccctcgg ggactaatcc
     1801 tcgaatacat cccccaaact gggctatttc tttaacaaca gttgagttta aataggaata
     1861 ctctgtacta gtggggagaa gaaaagtttc tatctctggg catatctttc gattggtaat
     1921 agcaatttga gtttcatatt cataatcaga tattgctctc aaaccgcgca gaatgatacg
     1981 gcaatttttt tgacgagcaa attgcaccag aaggccttga aaaacctcaa ccgttacatt
     2041 gtttaggtct tttatagctt ccaccatcat tgcttctcgt tcttccacag taaacaaagg
     2101 ctttttgtta ggatttttta aaagacccac gatcaaacca tcaaatattt tacttccacg
     2161 aatgataata tcaaggtgtc cattggtcac cggatcaaaa ctaccaggat aaatcgctat
     2221 caatatacct cttccttcct ttaagtatcc taattttcct caacctaatg gcagctcctc
     2281 ctatttattc aaagctacca aacaataacg cactatcgcc gtaagctcgg cgaataatta
     2341 cccgataaaa agggcattcg agcgaagggt tggatttttt tgaaaaacgg ataacaatta
     2401 aactgttttt tactaactca aataaattat acattttttc caatacttca acaggattaa
     2461 ttggaaaggg agggtcaaga aagacgattt ctggcggttc gactctggaa acccagcgtt
     2521 ttaagtcgaa aaaatccgct aatagaactt gggattgctc ttgaaaacca catagcttaa
     2581 cattccttcc aatcacggtg gtcattttta tatccttttc gacaaaaaaa accttgccag
     2641 ctcctcgact taacgcttcc aggcctatcg ctcctgatcc agcaaatata tcccatatga
     2701 tagatttttc caacgataac ggcattaaat caaacaaggt ttttcgaaga aatgcctgca
     2761 tcggacgagt cttttgattc tgaggcccga taatttttcg atttcttatc gaacctccgg
     2821 ttatatacaa gtatcccatg gtagaccttc ctgtttgaat aaatacttga tgaattaaca
     2881 aaaaaaccga taatataaat tgattaaaat ttcaagactg tgtataccaa accctggtaa
     2941 aaaatatttt aaaactgggg gcgcccaact caactacaat aacatctcct cttaggcttc
     3001 gatcattagt tcttccggta aaaattggaa aaaccgtctt tcaatttcct tcttgaggat
     3061 ttgattttcc ggtttctgca gtcggggatc attctggaga atcaaatcag cttcttgatg
     3121 ggctttttcc aaaattttcc aatcccgaaa aggatcggct aaatgaaatt ccaaaactcc
     3181 cgactgcctt accccataaa attctcccgg tcctctcatt ttgaggtcct cttcggcaat
     3241 ttgaaatcca tcatcggtat tgaccatgat ttccattctt ttttggatat cttgattctt
     3301 cccctggtat agcaaaacac accaaccagc ttgatctcct cgaccaattc tcccccgcaa
     3361 ttgatgaagc tgtgccaaac caaagcgctc ggcattttca atgaccatca cattggcatt
     3421 gggcacatcg atccccactt caatcaccga ggttgctacc aagacttgaa tttctccccg
     3481 agaaaatcga cccataatat catccttctc ttttgaagga agttttccat gtatcaactc
     3541 aatctgagag tgggaaaacc atttcttttg gagctgttca taaacttcat atacattgct
     3601 taattcaaga tcactttcct caattgccgg acaaactaca aaagcttgtt gtcccttctc
     3661 caaaaaggaa cctactcgtt gaaaggcttg tttttgttct ttcatagaaa aagcaactgt
     3721 ttcaaccggt tttctcccga ttggttttcc atcaagaacc gaaacatcca ggtccccgta
     3781 aagagttaag gctaaggttc ggggtatggg agttgccgac atcaccaaaa ggtgaggaac
     3841 aacggctttt tcttttaatc gggaacgttg ttttacccca aaacgatgct gctcatcgac
     3901 aatgactaat cccaatcgat taaactgaac ataatcttcc agcagggcat gagtcccaat
     3961 aacgaaatgt accctgcctt ctccgatttt ctctattatt tcactttttt ccttactttg
     4021 accaccggtt attaaactaa cttcaatacc aaactctttt aatatttgac tccaccgctg
     4081 ataatgctga tcggcgagga tttcggttgg ggccatcata gcaacctgct gtccggcttt
     4141 tataatatat agagcattaa tgatggcgac caaggttttc cccgaaccaa catctccctg
     4201 gagcaaccga ttcattattt tcccactggt caggtcattt tctatttgtt ttaatacttt
     4261 tttctgagct aacgtcaatt caaaaggaag ttgttgaata aaacggttga tgaatgtaaa
     4321 gtcgggaaca atgggttgaa cctgtaatgc ctgaatttga tgccttctaa cttttagact
     4381 taaagcaaaa agaagaaatt cttcaaaaat aagcgtaacc tgcgaccgag aacgatgctt
     4441 attcagttca gtaattgagg atcggctcgg atggtgaagt tctaaaattg ccccaggctt
     4501 ccttaggaga ctatattttt cttgaatagg tttaggaata gtatcttgaa aagcggctaa
     4561 ggatgtttta agtgctatat cgataagaga acgtaatttt ttggcgttga gtccatcggt
     4621 taaaccataa accggcagga tggattcata ttgaatatct tcggaatcga ttatttcata
     4681 ctcaaagcga ctggtttctt tctttccata agctgtttga aaaaaaccgg tgatgagcac
     4741 ttttttcccc ggcgtgagaa ttcgagccaa aaaatcacga ttaaaacaaa ctaaagaaat
     4801 ttgcccggtt tggtcttgaa tcactacttt caacaatcgt cctctccggg tagatatccg
     4861 atcgtgtcga accactttcc ccaatactgt ttgtaatcct gaaccttccg ctttagcaat
     4921 ggggataatc tgccctcgat cttggtaatg acgaggaaaa taaagaatga ggtcttctcg
     4981 ggtataaata tttaaacggt tgagctgttt ttcaacaact ggtccaactc cttttatggt
     5041 tcgaaccgaa agctggaaat cgaccggttt tataggcgat gaaggttgag aggcagaagg
     5101 ttgcggttgg tgtccatcta taatgcaggt ttccaagttt ttcagaaaaa tgctcctggc
     5161 tggttttgac atttggggat acctatttac acttaagaat atttcctcag gaactggaat
     5221 tgataggctt tggatcattt tttttacata ttgattaaat cccccgaaca cggtttgatt
     5281 atccagtcgt tgagattctt gttcaatgat tttttttagt cgatctctat ccttctccat
     5341 tttaaaaaca cccgagggta ttgtaacctg atatgagaac caagacaacg gaaagatttt
     5401 tatcccttgg taagaatgag ttgaattttc tatttccttt gtgctatatt actcgtcgaa
     5461 acttgaaaaa tgacgagaaa ggttggattc aaaatggcaa tctgtgaagt ttgtggaaaa
     5521 aaaccggttt ttggaaacaa gatcagccac tcccaccggg ttacaagaaa aatgtggaga
     5581 ccaaatattc agaaagtccg agtacggctt aatggtcatt cggcaaaaat gaacatttgt
     5641 acttcttgtc tgaaggctgg taaaataaaa aaagcgtaag aaaacttacg ctttttttat
     5701 tttcaaaaag tcagaaatct cctctaaacc gctattaaaa cctcttgatt ttgtcagaga
     5761 tctcacgtac tttttgggta attttgttct catcggaaaa caccagtgaa ccagcagcaa
     5821 taaccgatgc cccacaagat gctaagtatt caatattgtt ttcatgaata ccaccatcaa
     5881 tttcaattaa ggtatttaaa cctttcgatt taatcttctt cctcaactga ataattttct
     5941 ggtccatacc tggtatgatt ttttgtcctc caaaacccgg gttaacggtc atcaacaaca
     6001 ccagatataa ttttgggaga atctggtcaa gcatatgcac tggtgtgcca ggagcaacag
     6061 ccaatgaagc cttggcacca gctttctgaa taagattgat taagaaatca aaatcgggga
     6121 caacttccag atgaaagcta atattgtcag caccagcatt aataaaatca aggtaacaat
     6181 cctgggggtt ttggatcatg agatggacat ccagaaaagc tttcggcagt attttccgaa
     6241 ctgcatcgac gattaaggtc ccaaaggtaa tgttgggaac aaaatgccca tccatgacat
     6301 caatatgaat catctctact ccgccctctt gagccatgag gagttgatct tttagtcgag
     6361 aaaaatcaca agataaaatt gaaggggcta atattgccat aaacaccacc tattcaagca
     6421 ttttataatt ctttttccca ataataataa ccgtttaaat aaatcaggac tttccccttc
     6481 cccttggttc gggataaaat ttccaccgtt tctccacctt gatgttgctc ttcgtaaacc
     6541 acccgttctc ccaattcatc attcactaca atcctcacag taattggaac tctggcatcg
     6601 ggtaaagcaa atcgaaggct taaatcttgt aaatcttgaa tagcctgcac gacctgaaaa
     6661 tttattgcct ggccgacaga agcttcctgg ccggattctg gcatttggcc aataataata
     6721 ccgggatttc tttccggcat ggccacttct tcaatttgac caaccgataa atccatttct
     6781 gccaaaagcc gcctggcttc ttcaactttt aaaccaatta aatcaggaac caatattttt
     6841 gctggtggag ttgcagctat cgctgccgta ccgcctttgc tgattaaaag actgacttga
     6901 ctacctaaag gaagagtgct ctcggcaggt gggtcctgtg atataaccat tccttttaat
     6961 acatcttcat gagtcacttc caccactcga cccagttgat atccgttcgt ctctaacaga
     7021 ttttgggctt catttaaact taaatcccgg acatccggca ctctactcat cttgacttct
     7081 tgggctgcag ttggagcgac aactgctccc ccagcttggg cttgtccagt ttgtccgctc
     7141 acgacaactc tgatttttct tcctcgtttg gcatcagctc ccggcagtgg atcttgttcc
     7201 aatacctggt ttgccatcct gtcagaccgg ctttctactc gggctacttc tacttgcaaa
     7261 cccatttttg atgccaaatt cactgcacta accaagtctt ggttacggaa atccggcact
     7321 gataatgatg tcgcattgac agaatactgc aagagcatta atccaaaaaa accgctgaga
     7381 accactccca agccaaaggc aattaccgtt aggagtattc gtaaaaaaag gttcggtgtt
     7441 tttacccttt ttgcagtcac agtttcctcc aagccttccc aaaaaaccca tcggaattaa
     7501 aataatgagg ccatatccta tatgatttat catcggttaa taagatagtt gattgcaatt
     7561 caggttgcaa ctggctatct ccaggcaaat tcacttccca ttcctctggt ttaagttttt
     7621 caaaggctcc aactactccc tcggtttctt ccggagtcat ggtgcaaaca caatataata
     7681 taataccatt ttttttaatt aatgcagagg cttggcataa aagttgaaac tgcttttcag
     7741 cgagagattg attatcagac ttatttctta aaattccaat ttcggggttc cttcgcagcg
     7801 ttccccaacc ggaacaggga gcatcaacaa aaactcgatc ggaactaaat aattcttgag
     7861 gaaggggctg ggttgcgtca gctgcaaggg tttggataat atcaattcct tgacgatgag
     7921 cgagaacttc aagagttttt aatttttccg gatggacgtc tactgcatgt atacaaccct
     7981 gatttttcat aatctgggcc atcattaaag ttttcccgcc cgaaccacaa ccaatatcaa
     8041 caatcttctc acctggcttt ggggcaaaaa gatgtaccat gatctgagaa ctgaaatcat
     8101 ggatagaaaa atatcctttt tggtattcat cggtttcaac taattgagaa taatccaatt
     8161 gagtatttag agtattgggg agcatgggta gtgaatttac gacaatagaa tatttttttg
     8221 ataaggtttc aatcagtttt tgagaattgg ttttcaatga attcactcga agataaagaa
     8281 ctggcggaag aaccaaggat tttttaagtt ggttcatttc acccctggtt aagtaaatcc
     8341 tccaatattc ttccaaccat tggggaagaa ccatactcca gtgttcggct tgtttacacc
     8401 aacggattct ttccgccgca gctttacgtg agatggcatt gaccagttta atccagggat
     8461 gagaatgagt aagacgagct aattcaacag tctccgacac cacggcataa tcgggaatgc
     8521 gagtatctac tgccagttga aaaagtccta aatacaatat aatttgaacc tttttgggaa
     8581 gacttttagg tttttgaaga taagttgagg cgatttcttg aagctgagga taaaatttta
     8641 caattcctcg aactaaatga actaaaaagg aacgatcggg aattgaaaga tcgccagttt
     8701 ttattgatgc tgaatattct ttatctaaaa aaacttgttc cccacgaaaa agtcgattca
     8761 aaatacgaaa agcttctaaa cgaacattca cttttatgaa tataattcct ttcaattctt
     8821 tttttaaatt atttagttta attctcctgc tacaatttca attggtgaag gtagaaaata
     8881 ctgccccctt tcgccttaat ctcacccatc agggacaaaa aaactaaagg cagagagggg
     8941 agttgaaatc gttccagctc ttttgactta ccacacacgg tatcctcacc ggtcaatgct
     9001 tttgcttttt aatcccggct ttgacgaaga agaattaaac gcaataattg taaggcagcc
     9061 atggcggttg cagcaacata ggttaacgct gcagcattga gcattttttt aatatccggc
     9121 tgctcatcca cggcaactat accagaattg gttagggctg cataagctcg acggctggca
     9181 tcgtattcta ctgggagagt aaccacctgg aataaaaccg ccaagctaaa gaagataatt
     9241 ccaatatcca taagaaatcg aaaagaaaag attaacccta taaaaaagag cggaaaggcg
     9301 gcttgcgaac cgatactggc cactggaacc aaaatggaac gcaaggcaaa agccccgtat
     9361 ttctcgcgct tttgtaatgc atgcccaacc tcgtgcgcag caatgctata atctgctatt
     9421 gaaccacccc tagccacacc ggtagaaagc cggagtattt cccgagatgg atcatagtga
     9481 tcggttagct gccctggggt ttcttcaacc cggagggaaa gattattttg ttgaattagc
     9541 tgttgggcaa cttcaaatcc actcaaacca gcccgagaac gtttttgtga aagagttgca
     9601 taagtgcttt tcacttttga ttgggcataa aaagccagga tgagtgccgg tatcaataaa
     9661 ataaatgtgt aatcaaaagg ataaaacatc ttccgtacac ctcctactga aatagatctc
     9721 caggttgaat acggtatcca cagcaaaact cctgtccggt ttgaactttt ctattttctg
     9781 gatgtaatac ttctatcgct aatattccct taccagttcc caccaataat ccaaccttag
     9841 gttcatagcc aatgatttgt ccggctttca ctttttcctt ttgtttatag acgtgagctt
     9901 taatgacttt taggcgtttt tgatgaaaat atccaaaagc tcctggacag ggagcaaagg
     9961 ctttgacttg gcgaacgatt tcttcagctg aacgattcca tttgatttga taaagatctt
    10021 tcggaatttt tcctgcatag ctcgcttgct cattcgattg tggaatcggt ttgatctttc
    10081 cttcaatcca ggcaggcaaa acctccacta acaattctcc accgatttta cctaatttat
    10141 tttctaaagt aatactattt tctgaatctt caatcatcac ttcacgggtt gctaaaactg
    10201 gcccggtatc gattccttcg tccatcaaca tgatgctgac accggtcttt ttttcaccgt
    10261 tcaaaattac ccagcggatt ggatcagcac cacgatagcg aggaagcatc gaagcatgca
    10321 cgtttataca cccgaaagga gcaatttcta atagcgatga acgtaagatt tgtccgtagg
    10381 cagccacgat cataatgtcg ggagcaattt tattaatttc atctaaaaaa tctttccgat
    10441 tcactttatc gggttgtaaa actttgaacc cctgatcttg ggaccaaact ttaaccggtg
    10501 gagcggttaa ttttcttcct cttccacact cacggtcggg ttgggtaata acagctgcaa
    10561 tattatactg ggcatcgaca agagcgctca acactccaat cgataaggga gttgttccta
    10621 aaaaaactac ttttatcatt catgatcacc atttgtattt attcttgata atttattagc
    10681 caataccaaa cgacgagttg ggctcaaacg gtcaataaag agaataccat tgagatgatc
    10741 aatttcatgc tggatgatcc gtgccaataa gccatcagca aaaaaatcca gttgccttcc
    10801 ttgaacatct tgggtagtaa cttgaatttt ctggaaccgg ttgacctttg aaaatattcc
    10861 aggaacactc aaacaccctt cttctccaat ttctttttct ggatcgactt ctttccactc
    10921 gggattaatg aaagccatgt cttggttagt atttggattc gttaaaacaa ccaagcgctg
    10981 cagaattccc acttgattgg cagctaatcc caagccagag tttttatgca gagtttcgaa
    11041 catatcctgt atgatttttt tgactttgcc atcaatattt tgaactggtt cagctttttg
    11101 cctcaaaatt tggcttccat atttctcaat attcagatcc gccattgata tcaccttttt
    11161 ttcttatatt tcaaaccaac aataccataa tttattaatt taattctaaa acactaaacc
    11221 tggattcaaa accaggtttt caccctcatc ccaaccttct cccatcaaga aagaaggagc
    11281 aaattaacct atgaagaaag gagttaacca aactactcaa tctttaccat cggttgtcat
    11341 aaattggcac aaagtcttat cgggggtcat cctttaccca accaggaaga gtattataaa
    11401 ctttaaaatc aacttcaaca ccagtgcttt ttctggcttg aaccacagct gtcactttat
    11461 tataaagggt gctcaaaacc gatggttcaa actttatcac caattgatca ctccatcctg
    11521 atttcttgag gtaactatct ccaggaaaag gaccatcaac tataaactga ccaaccttta
    11581 attcattttt tactttattt agaaccattt cacgatttaa cttattcctt gaacgagcta
    11641 tcacctgaag aagcccctgg ctgggaggat atccatattt ttttcttttt tccaattctt
    11701 gtttataaaa ttctgcaggc ggaaggaaaa atgggtcaat atatttttga tattgtttag
    11761 aagtttcgat aagaactctg ttcttcggtg gttttgctgc tcccaaaaaa tagagagctc
    11821 gctggatatt ttgataaaat ttttcccgta cttgaaattc aggcaaaaat aaccaatctt
    11881 caaaggaatg aatgataaaa agagtcgagt gagctaaaag ttcttcatgt aaagctgctg
    11941 aggttccaac aataatcaac ggttcctcgc cctttacatc cagctcgctt ctttcttgat
    12001 cagaagcgat ttttactatg ggtactcctg agaagatcct ttttaaaaat tgataaactt
    12061 tctcaatacc atcaccccaa gttttcatcc agggcccatg acattccgga caaaagcttt
    12121 cggggataac ttttttccca caccgagggc agcgaagaat cctctctttc cggtaaaatc
    12181 tcaaggctac atcacaatcg gaacaggaat aataaaaacc acaatcggaa caacccaaag
    12241 caaccgaata tcctgttttt tgaacccaga taatgctatt ttgccggttg gttatatttt
    12301 gagcaatggc tttttgtacc aattgtggga gaatagtctt tttctgttca taggttacta
    12361 gctccagctt ctgttctttg gtaaattgtt ttacttgtcc tttttgggtt tcaaaatatc
    12421 cttcctgcaa tccaaaataa agggttagat cgggtatggt tccaataata tgcagatccc
    12481 caccagtaat actaactttc tcaattaacg aatgaaaaga atgataatgc ggttgctgat
    12541 cagagtaatg accaaagtcg tcgggatcaa acaagacctg aacaccaatt tgatgaattg
    12601 gtaaaaattg agccaaacga gtgcccagca aaacgtcaaa atagccttct cttaccattt
    12661 gataggcaag cattcttttt cgggctgtta atcttccatc gaaagaaaga aaacgagcca
    12721 agggataatt agtttttaaa ttttccataa aataatctaa aatctttgga ccgggaaaca
    12781 aaatgaagat ctttaagcct cgatcgaagc tatctttgat tagatctccc aagagattca
    12841 aactctcggc tatagttccc ctttgccacc attttttatt ccatttttgc gggggaaggc
    12901 tatattgatt tttcttatag tcttcttcaa caaccttgat ccgattcttt tttatcaatt
    12961 gattgatttt ttgagtagtg agtcccaata aatctttaaa aataaataaa ttcactcctt
    13021 gggaaatggg atcaacacca tgctttttca aatcaagaaa atattcgtca tcttcattta
    13081 ttaaaaatat tcgctgactg gtccatttct cccacgacaa ttgatacttg ggaacaaaga
    13141 gagccagtgc ctctccaaga ctggaaaagg tttgatttga aattcttttg gcaaattcca
    13201 tctgccagtc cgctaagggt tcaagcggaa gacaggaaag caggcttttt aattcgtatt
    13261 ctttatcatt ttgaactgaa tcaaacgacc aaacgcatcc aaaacggcgt gatccatgaa
    13321 attcaacttc aacaatggtt ccaatctgaa ttttatcgct catctcttga ggaatacgat
    13381 agctaaacac tttgtagact ggaagcggaa gagcaaccga taccgaattc attttcatgt
    13441 aaaataaaga gaaaccaggt agttcacaat cgtttcagcc aattccattt tatccattaa
    13501 aggaatatca atcaatgcac cattcttatt gagaagccaa gctttatttt tattcacttc
    13561 gaaaccgctt tctcctggta aaatacgatt ggcaatcatc acatcacaat tcttttgctg
    13621 taattttcga cgagcttccg agacaatatc tccatcttca gcacagaatc cgactaataa
    13681 ttgagctgct ttcttttggc ctagttcgga aagaatatca ggattttgag ttaaacttag
    13741 agcaatttca cttttaccct gcttttttat ctttattgga gaggtaattg aaggtcgata
    13801 atcagcaacc gcagcggtca tgatacaaat atccatttta tcaaattgtt tgagagtcgc
    13861 agtttctaag tcctgggttg aaacgattgg aaggtaatca actccggatg gaattctcac
    13921 cgacattgga ccaccaatca aagttacctc ggctccatgg gctcgagcag cagccgccag
    13981 cgaacagccc atcaggccac tggaagaatt ggttagataa cggatgggat caatatattc
    14041 ccgagttggt ccggcagtaa tcaatgctct tttcccttgt agagatcgat aagaactaag
    14101 gaagtagtaa aggttttcta taaactcctc ttgatcggga agacgccccg ccccggtttc
    14161 accgcaagct aaagatccgg aagagggatt catgacccgt accccgatcg caatgagttt
    14221 ttttaaattt tcttgaacga ttgggttacc ccacatgtca acattcatag ccggaactaa
    14281 aatgattttc ttcggatcaa ccaaaaaaca gctgcttaac aggtcatcac caattccccc
    14341 ggccattttg ccgatgatat tcgcagttgc tggtgcaact accaaataat catattctct
    14401 cattaaatga atatggagaa ttttttcgcc ggattcaaat agactggaat aaaccggcct
    14461 acgggttaat gcgttgaagg tattttctcc tacaaatttc ttggctgcct cagtcataat
    14521 gacttggata tccgatcctg cttgaaccag atggctgata aaaccagcta ctttataggc
    14581 agaaattcca ccagtaattc caaaaagaat ttttttattc ttccaaaaca acggtggata
    14641 ataaatattc atgccgtttc cttagaccac ttaattttat cctcagcaac ctcttttaaa
    14701 gcaataaaaa gtggtttggc tggttgttga ttttcaatca aaggtttggc accttgatta
    14761 agttgtttgg aacgcttagc caccagggtg gttaaataat aacggttccc agttttcttg
    14821 atcaattcat caatggacag cataatttca ctccaatcta tctattttat cctgaaaaaa
    14881 aacgggcttg ataacaagct tctacaaata atataaaaat gtaagggcgt tgctccagtt
    14941 catgataaac ataagtaaga aaaaatgtca ttctgataaa agttcttgtg tctcatcatc
    15001 tccataacga atacgacaac gctcggcaat aataactgaa tggaatttat gaacggtttc
    15061 tttaagaaca ttattaatta tcaaataatc ataatcctga atcgatttca tttcaatcaa
    15121 agcattcatc atcctcagat aaataacatc ttctcgatca gttttacgat tacgaagacg
    15181 ttctccaaga gtttctaagg atggtggagc aataaaaaca aaaattccct gagggtaatt
    15241 tctttttact tggagagctc cctgtacgtc aatttctaat atgacatcaa atcctctctg
    15301 caaccactcc tccattttta aacgaggtgt tccataaaag ttaccatgaa ccttggccca
    15361 ttcaataaac agatggttct ctttcatgtg ctgaaaggtt tcatcatcga caaaatagta
    15421 atcaattgcc tctcgttctc cttctcgtct taagcgggtc gtgtaagaaa tagaatattt
    15481 taaaccttca cactgttcca ttaccgcctt tcttacggta ctttttccag caccagatgg
    15541 tccagaaaga acaaaaagaa ggccgggcat aactatcttt tactccacat tctgaatttg
    15601 ctctctcatt ttttctaata ccgtttttaa atcaataatt aaagaagata tttcaacatt
    15661 tgaagtctta gaaccaattg tattcacttc acgattcatt tcttgcaata caaattcaag
    15721 ttcacgtcct attgaatctt gacgggaaag aatatcatca aaccgacaaa tatgagcttt
    15781 caaacggata atttcttcgt gaatatcgct tcgatcaaca tagaatacta tttcctgtgc
    15841 taataagttt tcgtttaccg gaactgagga aatgatttct tttaatctct gattaagctt
    15901 ttcccgatag tactccggga tattctttga tttctcttca accatagata tcaattctcg
    15961 aaatttttcc atttgctgta taaggtcatg ctgcaacttt tctccttctc gtaaccgata
    16021 ctgtagaaaa gcttccactg cttctttaaa tattgggaaa aaccgctgat attcatcaag
    16081 ccagcggtga tcgtcttctt taagtttgat aatttccgga acttgcatta aatcccgcag
    16141 gtcggcttca taaggaatgt tcaattcgct gctcaattta tgaagagctt ttaaataggc
    16201 tttagctaaa agcgtattgg cttcaaccga aaatgcttct tcactgggct caaagttaag
    16261 ctttaactca acctttcctc gagatacccg ttctttaaga taagatatta aattttcttc
    16321 ccaaacacat aattcacggg gaagccgtaa attaatatct gaaaagcgat ggttaaagct
    16381 tttgatggat attcgataaa atccaatcgt accctcacct tcagcatgcc caaatcctgt
    16441 catacttttc attttttcac ctttcttaaa tctttttaga tatatcacca agttccccgc
    16501 tattatcacc ttatgggaac gtgatttaat tattggataa aacgattttt aaacactctc
    16561 tccgcagcaa tgtgacatct ttccaaagaa gaaaattatt ttacccatgg tctcctttac
    16621 tttttaatac tgatgactga acataatatt tcaatcttta atttgagccg tattcactgg
    16681 gaatgaaaac aaagcttgca attatacaac tggaataata ttatttaata ttattgatcg
    16741 tgtcaacaaa atcaggactc cgaatcgatg agaatactgg aaggaattcc tttacgcttt
    16801 caaattgaaa ccgtttcccc ccttgtggaa ggagctcggg tacaaaaaat tacccttact
    16861 gaaaaccgag aaacttgttt agagattcgc tctcgaaacc aaccgggttt tttagtccta
    16921 tccactcatc ccgaaatttg tttcttttac cattctcagc aaaaaccgat tatgaaaaaa
    16981 atcaaagaat cagcctgggt gcagattctc aataaatatt tggttggagc taaaataatc
    17041 ggattcgaac aaatgggatg ggaccgggta atccgtttaa cctttcagaa ccaaaaaatt
    17101 tgggtagaaa aaaacctctt ttatctttat tgtgagctaa ccggacgaaa cgctaattgt
    17161 attttaacga ccgccgacga cccttcttat atactcggtt gtcaaaaaat agttggtcct
    17221 caacagaacc gtttccgcac cattgtcgtt ggaaaaccgt atcagcttcc cccgcttaaa
    17281 ataaatgcca tagatccact cctcttggtt caggaaaaag ccgaatttcc aattccaaag
    17341 caagatgatg atttaccaaa atggctttta aaaaatattg atggtgctgg acctttcttg
    17401 gcaacgataa tagctgagtg tttagtcaac agcagattca atgctggtaa tcttcaattg
    17461 ctcttaagca cctgtcttca aaatatcttt cgaccacttt ttaatcaaaa tttggttttt
    17521 tccgtctata tctctccgaa ggataatttt cccttaggaa tattttggtt gtcaacacag
    17581 cattatgctc agcttcctca ccatgatttc cccaacctca atgaagcagt aaaatacttc
    17641 gctcaaacct actggagttt acaggctttt ctcagtcaaa aaaacaaaaa gaaaagtttt
    17701 atcgaaaaag aacttttgtt cattaaagaa gaattattga aagttggtca atctctggtt
    17761 agcgaggatc agctggttga gctattaacc cggggaaacc ttttgaaact ttatccgcaa
    17821 ttaaatatta ttaaaaaaca acccaaagga tttttggtag ccaaccttct cactggaaat
    17881 tcaggagaag tttttatcga gattgaccct cttctttcca ttaatcaaaa catgcagctc
    17941 tattttcgaa aatatcgaaa atcaaaaact cgcaatcaaa tactaaaaaa gaaaaaagaa
    18001 gagctggaat tccgtaaaaa aatccttgaa aaggaactca taaaccttga ccaagccccg
    18061 ctccaccaag agcaagatga caataattca acccaatcag gattttttga accgggtatt
    18121 ataaaattcc ttactccatc gaaaaattta attttggttg gaaaaaatga aaaagccaat
    18181 catttattag ttacccgttt ttcatcccga gacgattatt ggcttcacgt aagagatttt
    18241 cctggttcgc atgttttact gagaatatcc aaccagcccg agaggcttac tgaagacctt
    18301 ttcctggccg ctcaaattgc tgggtattac tcttctcttc ggaatgaaac ttcggtaatt
    18361 gttatgtata cacctattaa aaacgtaaaa acacttcctc atgccggctt agggaaaatg
    18421 atcttccgca acgaaaaaaa tttaaccgta attcctgcta ttcccgataa ccttcataga
    18481 atttgaaatt ttatttatgg cttcttgaaa atcactgggt aaggcagcgg caaaaaacat
    18541 ttttgtcttg gtaactggat gggaaaaggc tatgagaaaa gcatgtaaag cttgacgttt
    18601 cattatctca ccggaacgat tacttccata ggacttatcc cctacaatcg gatacccttg
    18661 agaacttaaa tggactcgaa tctgatgagt tcgtccggtc cgaggtttga ccaacaaaag
    18721 agaatatgaa tcaccaatcc acagaggctc tatctcagtc agtgctggtt ttccaccact
    18781 tgacgaaatc ttcatcaaac agggcacccg tggatttctc cgaatggcta aatcaacctg
    18841 cttaatcccc ttccactgtc cttcgattaa agctaagtaa aatttctcga ccgttcggtt
    18901 tttaaacatt tggatcagtt cgagataggc acgatcggtc ttggcaatca ccaaaacacc
    18961 cgaggtatcc cggtccaagc gatgaacaat tcccggacga agcggagctc cataacgaga
    19021 cagttcaatc ccatgaaaaa gcaaactatt gatcagggta tctttttgga aaggacgaac
    19081 tggatgaacg attattccgg ctcttttatt tatcaccaca atatgatcat cttgataaat
    19141 gatatctaag ggtagatttt gaggctctaa ggtgtgtcgt gcatcgactg gccaggtgat
    19201 ttcaagaata tcgccagggc ggacttcact actcggttta tatataactt cctggttctt
    19261 ttttatcaaa ccttgtttta taagccttga aaatgccgat cgagaaaaat ctggaaaatt
    19321 tctatgcagc cagatatcaa tccggcagaa ctcattatca tacaccttga ttttttgttt
    19381 ct
//
LOCUS       DAOSOL010000002        13344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_4658_length_13344_cov_4.158477, whole genome shotgun sequence.
ACCESSION   DAOSOL010000002 DAOSOL010000000
VERSION     DAOSOL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_13344_cov_4.158477"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..1253
                     /gene="fabK"
                     /locus_tag="PK171_00100"
     CDS             309..1253
                     /gene="fabK"
                     /locus_tag="PK171_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HOT04636.1"
                     /translation="METRVTKLLGIDYPILQGGMAWVSTAPLVAAVSNAGGLGFIGAG
                     GMDADELRENIHQVRIKTEKPFGVNLMLLSPHINEQIEVVKEERVPIVTTGAGNPSRL
                     IDDFSKRGIVTIPVVASVQLAKRLVRHGIQAIIAEGMESGGHIGEITTMCLVPQMVDA
                     LDIPVIAAGGIGDGRGMAAAFALGAEGVQVGTRFVCSNECNIHPYYKEAIVNSQERST
                     IMTGMSTGHPVRCLKNKLTRKFEELETLRASREEIEALGSGSLRRAVCEGDVEEGSVM
                     AGQISGLIQDIKPVSEIIQTMLIEFQNTLTRLALFKKE"
     gene            1261..2199
                     /gene="fabD"
                     /locus_tag="PK171_00105"
     CDS             1261..2199
                     /gene="fabD"
                     /locus_tag="PK171_00105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOT04637.1"
                     /translation="MDEWVFLFPGQGSQRVGMVQDFLDHYPHQTRGFFNIAQEKTGVD
                     LLKLSLDGPEEELNLTYNTQPALLTLSALIYQVMSSTNSFIPVAVAGHSLGEYSALIA
                     AGSINFSDAVYLVRKRGEIMQDAVPAGNGTMVAVIGLPDQQLEPLIQELSHNGKFEIA
                     NYNSSEQVVFSLEKTLVPLIMEKAKSRGAKKIIELRVSAPFHSSFMREAAREFQAILS
                     QISISRPQIKFLSNVTADYADDPEKIRELLNQQMVSPVRWKEIMDRLYQDGYRKFVEI
                     GPGNVLSKLFKREYDQVETRALQSVTALNEWMKEAS"
     gene            2201..2950
                     /gene="fabG"
                     /locus_tag="PK171_00110"
     CDS             2201..2950
                     /gene="fabG"
                     /locus_tag="PK171_00110"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF004198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOT04638.1"
                     /translation="MVDLKNQIAIITGAGRGIGFATARILGLYGATPVICDVLSESEL
                     TQAIEHLQADGIGAFSYRVDVTNKEQIDKMVLDVLERWGQVDILVNNAGLTRDAALLR
                     MKDEDWHLVLNVCLQGTYHCTKSVLKSMVKRRYGRIINISSVVGVVGNAGQVNYSSAK
                     AAIIGFTKSLAREVASRGITVNAIAPGFFDTDMTRNLPEAIKEGWINQIPVGRLGQPE
                     EIAEAVAFLSSQAAAYITGQTLHINGGMVMY"
     gene            2980..3213
                     /gene="acpP"
                     /locus_tag="PK171_00115"
     CDS             2980..3213
                     /gene="acpP"
                     /locus_tag="PK171_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT04639.1"
                     /translation="MDVYSKVKEIIVDQLGIEEEDVAPDASFIDDLGADSLDIVELIM
                     AFEEEFDIEIPDEDAEKITSVQEAIEYIESKLS"
     gene            3228..3944
                     /gene="rnc"
                     /locus_tag="PK171_00120"
     CDS             3228..3944
                     /gene="rnc"
                     /locus_tag="PK171_00120"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT04640.1"
                     /translation="MPFNEAELIELEHRIGYHFENRSLLKTALLHKSALEGKEGHCND
                     KFEWLGDAVVGFYIADYLFSQYEKPRSWLSAMKAKWASEESLAQVARNIHLERFILLG
                     KGEERGRGREKNSIISSALEALVGAVYLDSKSFDTTKKVLDTIFSSEGGLELVFLSVN
                     YKSLLQTWSLKEHETLPAYQVIEESLDRKIYRIAVLINEKEIAIGEGTNKKKAEQEAA
                     RKAWEKIIEEKYGFRDNPCF"
     gene            3935..7102
                     /locus_tag="PK171_00125"
     CDS             3935..7102
                     /locus_tag="PK171_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT04641.1"
                     /translation="MFLTHLNVQGFKSFAHPITIEFHPGLNVIVGPNGSGKSNVIDAL
                     RWVLGDNFREIRVSQGKEVIFHGAAGAKPLGMAFIETQWSDSEEPPYSIGRRIFASGE
                     SEYFLNQNRLRLKDLKEELRKLGFLVDSIGVAVVDNTKLQSLFEFRPSDKFSLFEMAS
                     GTYAVKEKLASVKSSLTRIGEKVSRLKERENELNLQIEKVSEHARQEEKYLSEEKLFI
                     ALRKEYFNLLIQQRLKKIKGLESEKEEVEKELKHLGEEGVGLDLLFNKQQELLQEKEK
                     LKSQILERLESLREELRSLEQQIYFHLTEARQREKNKLLNREALNELEPKLALLRKNV
                     EDLRNNPLYHETLDELEEKEKEFQKKVSEFRIRKDSYNEQINQLKQEWSRLETSIEYL
                     TEELASIDREQSSLDFSSTELVQQIESLQIQLSTLQEESRKFIKKREQLKEKLDRKKA
                     LLVKIRNGLREYEPEEKIDQRIADLSKNLIQKGWSGKVVYAFNWLFKDYTAILRTEGN
                     LNLKDSLPKTGQGFVYLDILPPSNYWKIFTKKQIIDALKNREVPQVNMISSDGNIVYR
                     RDGVLIFPRKLITNQSGVRFYRSWQKRGAALQKRIQEGEKDISGYLVKENQLEKELTK
                     IEGELQVLQLRYDDLQKEKKKKIEKIELINLKKQSFLSEKEILGEKLDRLGWEKEDID
                     NEVNQLEDELREIHNQRFEREKLRSTEDKLQGELTTIEMLIEKSVFSLQNIENSRNQS
                     LDIWKEIIFKIKKLNEEHQFELNRKKVSFAAVDEIRERCNDIELQREKRKDRQDSLSY
                     KREKLYLQKEKWDFEIQEYTTRLDEYQEWQTDDLPLPSFTDIHHLEQMITEKENRLKT
                     WEIRRGSISQLRDLKERQNYLKEKLTFFQDAMARFEKNRIECELLCQELFNEFLLEVN
                     FHFQKNFQRVFNGGRAKIEIEEQNLEIVIQIPGKKKQRLSLLSSGEKALTALCLFFAL
                     FKSGGYRFCFFDEVDATLDHFNSVRLADLMKEFSHECQIIIVTHQEEIMEASDRIIGV
                     TMDKPGISRVVTLSGKNLSLLSSQN"
     gene            7167..8102
                     /gene="ftsY"
                     /locus_tag="PK171_00130"
     CDS             7167..8102
                     /gene="ftsY"
                     /locus_tag="PK171_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT04642.1"
                     /translation="MEEKKGFFHKWFKGVEKIKEGVKQQFSRLWSADSLQDEDWEALE
                     EMLVQADLGPNLAMELVEEFQEIKKQKAENGNWQDWLYGKLGSILDGNDSSLFSSAQS
                     GAMKAILLAGINGVGKTTLAGKLARYYSLQGEEVALVAADTFRAAAVDQLEIWAERAG
                     CFFFKGSPGADPGSIIFDGIEAARKKNRGFVIIDTAGRSHVNKNLLAELEKMGKIVQK
                     NFSPENIENLLVIDAMAGQNAFLQAESIGKAIPLNGVILSKWDSQAKGGIVFRIKKEL
                     ELSIKFLGIGEKIEDLQPFDSDQFVKAIVYDEEVS"
     gene            8175..9512
                     /gene="ffh"
                     /locus_tag="PK171_00135"
     CDS             8175..9512
                     /gene="ffh"
                     /locus_tag="PK171_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT04643.1"
                     /translation="MFEQLINKFSGIFKKLRSKGKLSEQDVDSILKELRMVLLESDVH
                     YRVVKDLIARVKERAVGREVLESVTPGEMVIKILWDEIRKVLGGNVSSLETGGATPVL
                     IMLVGLQGSGKTTTLGKLAVRLKNQGLFPLAISTDTRRPAAKEQLAILCHNSGIDFYD
                     DSKALTPLQMIQNAIRHSREKSYDVIFIDTAGRLHVEEELLAELKEVVDTIPLKEILL
                     VLDATTGQEAVKVAQSFKEWVNPTGLILAKVDTDARGGAVLSIVAQTGWPVKFAGVGE
                     KVDQLEVFHPDRMASRIMGMGDTLTLIEKIERSIDEEEKEKIEKKFGKKELDLNDFME
                     ELKRVKEIGSFDEIVGMLPGNLRGGISQIDGEKNIKRVQAIIQSMTPAERINPSLINS
                     SRRRRVAQGSGTTIQDVNRLLKQFEDFRKLWKQMNRTGKNPFSKRKGWFGWNA"
     gene            9583..9834
                     /gene="rpsP"
                     /locus_tag="PK171_00140"
     CDS             9583..9834
                     /gene="rpsP"
                     /locus_tag="PK171_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT04644.1"
                     /translation="MAVKLRLTKIGRKHAPYYRIVAIDSQKARDGKPIEILGNYSPIV
                     NPPLWSINFDRIQYWLNNGATMSDKVKTLLKQTERPGIE"
     gene            9831..10061
                     /locus_tag="PK171_00145"
     CDS             9831..10061
                     /locus_tag="PK171_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOT04645.1"
                     /translation="MKDLVEYMVKSLVDNPEQVKVSEVQGEQSVIYEVRVAPEDMGKV
                     IGKQGRIARSIRTIVKAACVKTGKKAVVEILE"
     gene            10073..10567
                     /gene="rimM"
                     /locus_tag="PK171_00150"
     CDS             10073..10567
                     /gene="rimM"
                     /locus_tag="PK171_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT04646.1"
                     /translation="MVVGKFIKPHGLAGGLKALPFGETLNSFSPGTILFVEDQDQYRT
                     LVVKDFQPMGRFVLLWLRDLSTKAEVESLSGQLIWREKSQVSVTDTPDSYFVYQLIDS
                     KIMENGQSLGVVSDVIEGPAYDYLQVNRDNREFLIPFIRVYIKHVDLQGGYITVDCPK
                     GFWE"
     gene            10564..11274
                     /gene="trmD"
                     /locus_tag="PK171_00155"
     CDS             10564..11274
                     /gene="trmD"
                     /locus_tag="PK171_00155"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT04647.1"
                     /translation="MKLRIDIITLFPELIRCFAETAIVKRAQDRGLVFIKAYNLRAFS
                     SDRYRTVDDYPYGGGPGMVLKPEPISRAVEFIKGVTHSQPRVLITSPQGRVFNQSLAE
                     ALSLCPHIILVCGRYQGIDQRVIELCQGEEISVGDYVLSGGELPAMIVAEAITRLVPE
                     VLGDEESIHFDSFYDRILGPPQYTRPRIFEGRAVPEVLTSGNHSLIEEWRRKKALQKT
                     RLVRPDLLDKLSDEVIHE"
     gene            11419..11772
                     /gene="rplS"
                     /locus_tag="PK171_00160"
     CDS             11419..11772
                     /gene="rplS"
                     /locus_tag="PK171_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT04648.1"
                     /translation="MDKARLLHSVESAYIKEIPDVEPGQTVRVHLKVVEGDKERIQVF
                     EGLVIALKGSGISRTMTVRKMSFGIGIERVFPIYSPRIETIEIVRRGKVRRAKLYYLR
                     GKSAKESRIEEKRER"
     gene            11786..12292
                     /gene="lepB"
                     /locus_tag="PK171_00165"
     CDS             11786..12292
                     /gene="lepB"
                     /locus_tag="PK171_00165"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT04649.1"
                     /translation="MREIVETVFWALLIAFIVRYFVVEGYYIPSSSMKPTLIPGNRVL
                     VAKFYYRFYRPHRGDIIVFRFPSNRKKNLIKRVIALPGETVRIENGLVHIDGIPLQGD
                     QFNREYFNVGNYGREEQVIPENSYFVLGDNSHNSDDSRFWGYVPFKNILGRAFLVYWP
                     PRSIQILR"
     gene            12295..12888
                     /locus_tag="PK171_00170"
     CDS             12295..12888
                     /locus_tag="PK171_00170"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT04650.1"
                     /translation="MLEKDLWVKGFKYVGGVDEAGRGPLAGPLVSCCVIFPPGCIPSG
                     LNDSKLLSPAQRNHCFQLICRTAFSLGVSIVGEKMIDCLGIQSANLYSFREALMRASV
                     KRTPEFVIFDWLKIPELDIPSISISHAELKSFSVAAASIVAKVVRDSLMEKQYQELYP
                     EYGFIHHKGYGTKLHRDKINQLGIANCHRRSFCGKYL"
     gene            12872..13246
                     /locus_tag="PK171_00175"
     CDS             12872..13246
                     /locus_tag="PK171_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT04651.1"
                     /translation="MVNTSKRIGQMGEEIASRIVIRQGKMKIIERNFRSRIGEIDIIA
                     LDKNTLVFIEVKSRAVLNAGFPEESVTKYKQDKIRKVALYYLISQGYNPNHTNYRFDV
                     ISITFNGDDYAHPSVKYYINAF"
BASE COUNT         4292 a         2237 c         3046 g         3769 t
ORIGIN      
        1 actaccaatt tgcatgagat gagaatgaaa acccggaaat ctttaaactc cttctccctc
       61 acttttttcc tctcccacca ggagagagga aaaaaagagg aacgagggct caggatgaca
      121 cctgcggtgt cagatgagat ccttatgtgg aatagcgccg ttcaggtaaa cgactttatt
      181 attagatata tgagccatga cctcgtagtg ggaggtttta aggaaaatat tctattaata
      241 ccatttcgca tctaaaaaga taagggatac tgattattat agctggactg aaacgaagga
      301 gagatagaat ggagactcgt gtcaccaaac tattaggaat agattatccc attctgcaag
      361 ggggtatggc ttgggtttca acagctcccc tggtagcagc agtttccaat gcaggtgggc
      421 tggggtttat tggagctggt ggaatggatg cggatgagct tagggagaat atccaccagg
      481 ttcgaatcaa aactgaaaaa ccattcggag tgaacctcat gcttctttct ccccatatca
      541 atgaacaaat cgaagttgtt aaagaagaac gagtcccaat cgtaaccacc ggcgccggga
      601 acccatctcg cttaattgat gatttttcca aaagagggat tgtaaccatc ccagttgttg
      661 catcggttca attagcgaaa aggctggtcc gtcatggtat tcaggctatt attgccgaag
      721 gaatggaatc gggaggtcat atcggagaga taaccaccat gtgtctcgta ccgcaaatgg
      781 ttgatgcgct tgatatccca gtcattgcgg caggaggtat tggtgatggc cgtgggatgg
      841 cagcagcatt tgccctggga gcagaaggag tacaggttgg aacccgtttt gtctgttcga
      901 atgaatgtaa tattcatcct tattacaaag aagcaatagt caattcgcaa gaacgatcga
      961 ccataatgac cggaatgagc accggtcatc cagttcgttg tttaaaaaat aaattgacca
     1021 gaaagtttga agaattagaa acacttcgag caagccgaga agaaatagaa gctttaggtt
     1081 caggaagttt gcggcgagcc gtctgtgaag gcgatgttga agagggttcg gtaatggctg
     1141 gtcaaatttc gggattgatt caggatatta aacctgtttc tgaaattatc caaactatgc
     1201 ttatagaatt tcaaaatacc cttactcgac tcgccctttt taaaaaggag taagtattca
     1261 atggatgaat gggtatttct ttttcctgga caaggatcgc aacgagtggg tatggtgcaa
     1321 gattttttag atcactatcc ccatcaaacc agaggttttt ttaatattgc tcaggaaaaa
     1381 accggagttg acctcctcaa gctttcttta gatggtcccg aggaggagtt gaacctgacc
     1441 tataatactc aaccggcgct tttaaccctg agcgcactca tttatcaggt aatgagttca
     1501 acgaattcat ttataccggt tgctgtagcg ggccatagtt taggtgagta ttcggcattg
     1561 attgccgccg gttcgatcaa tttttccgat gcagtttatt tggtaagaaa aagaggagaa
     1621 attatgcagg atgcagttcc ggcagggaat ggaacgatgg tagcggttat tggccttcct
     1681 gaccaacagt tagagccact gatacaggaa ctctcccaca acggcaagtt tgagatagcc
     1741 aattataatt cctcggagca agtagttttt tccttggaaa aaaccttagt tcctttgata
     1801 atggaaaaag ccaaatcgcg aggggctaaa aaaattattg aattacgggt tagtgctccc
     1861 tttcattctt cttttatgag agaagcagct cgtgaatttc aagctatttt gtctcaaatt
     1921 tccatatctc gacctcagat aaagttttta agtaatgtta cagccgatta tgccgatgat
     1981 ccagaaaaaa tacgagaatt actaaatcaa caaatggttt ctccagttag atggaaagaa
     2041 ataatggatc gactctacca agatggttat cggaaatttg ttgaaattgg gccgggaaat
     2101 gtgttatcga agttgttcaa gcgggaatac gatcaggttg agacccgtgc gcttcaatca
     2161 gtaacagctt taaatgaatg gatgaaagag gcgtcataaa atggttgact taaaaaacca
     2221 aattgctata atcaccggag ctggtagagg aattggtttc gcaacggcaa gaatattagg
     2281 tctttatgga gcaactcctg ttatatgtga tgttttatcc gagtcggagc taacgcaagc
     2341 gatagaacat ttacaagccg atggcattgg tgccttttcc tatcgggttg atgtaaccaa
     2401 caaagaacaa attgataaaa tggtactgga tgttttagaa agatggggcc aagttgatat
     2461 cttggtgaat aatgctggat taaccagaga tgcggccctt ttaaggatga aagatgaaga
     2521 ttggcactta gtgctcaatg tttgtcttca aggaacatat cattgcacca aaagtgtttt
     2581 gaagtccatg gtaaaaagga ggtatggtcg gatcattaat atatcttcag tggtgggggt
     2641 agttggaaat gccggacagg taaattactc atctgccaaa gcagctatca ttggttttac
     2701 caaatctctt gcccgagaag ttgcatcccg tggaatcacc gttaatgcaa ttgcacccgg
     2761 tttttttgat actgatatga cccgtaactt gcctgaagca attaaggaag ggtggatcaa
     2821 tcaaataccg gttgggcgat tgggacaacc agaagaaata gctgaggccg ttgcctttct
     2881 ttcatcacaa gccgccgctt atattaccgg tcaaacgctt catattaacg gtggtatggt
     2941 gatgtattaa aactaattga atggttggag gtgcaggaaa tggatgttta ttccaaagtc
     3001 aaagaaataa ttgttgatca gcttggtatt gaagaagaag acgttgcacc agatgcttcc
     3061 tttattgatg acctgggcgc tgattctctt gatatcgttg agctcattat ggcatttgaa
     3121 gaagaatttg atatagaaat tcctgatgaa gatgccgaaa aaattacctc agtacaagaa
     3181 gcaatcgaat atattgaatc aaaattaagc taattaagaa accgacaatg ccttttaatg
     3241 aagccgaact catcgaactt gaacatcgaa ttggatacca ttttgaaaat cgttctcttt
     3301 taaaaaccgc cttattacat aagtcggcgt tggaagggaa agaagggcat tgtaatgata
     3361 agtttgagtg gttaggtgat gcggtagtcg gtttttatat tgccgactac cttttttctc
     3421 aatatgaaaa accacggagc tggctttcag caatgaaggc taaatgggcg agcgaagaaa
     3481 gcttggctca ggtagctcga aatatacatc tggaacggtt tattcttctt gggaaaggag
     3541 aagagagggg tcggggaagg gaaaaaaatt cgattatttc cagcgcattg gaagcattgg
     3601 ttggtgctgt atatttggat tcaaaatcct ttgatacgac aaagaaggta ttagatacta
     3661 ttttttcttc tgaaggcggt cttgaattgg tttttctttc ggttaattat aaaagcctct
     3721 tacaaacctg gtcacttaag gaacatgaga ctctcccagc ttatcaagta atagaagaat
     3781 cattggatcg aaaaatctat cgaattgctg ttttgattaa tgagaaggaa atagccattg
     3841 gtgagggtac caataaaaaa aaggccgaac aggaggccgc ccgtaaagcc tgggaaaaaa
     3901 tcatcgaaga gaaatatggc tttcgtgata atccatgttt ttaactcatt taaatgttca
     3961 gggttttaaa tcatttgctc atccgattac gattgagttt catcccggtt taaacgtcat
     4021 tgttggccca aatggttcag gaaaaagtaa tgtcattgat gctctccgtt gggtgttggg
     4081 agataatttt cgagaaattc gagtttccca ggggaaagag gttatctttc acggagcagc
     4141 cggagcaaaa ccgctaggaa tggcttttat tgaaacccaa tggtcagaca gcgaagagcc
     4201 tccctattct attggaaggc gaatctttgc atcgggagaa tcagagtatt ttttaaatca
     4261 aaatcgcctt cgtttaaaag atttaaaaga agaactgcgg aagttgggtt ttttggttga
     4321 tagcattggt gtagcagtgg ttgataacac caaacttcag agtttgtttg aattccgtcc
     4381 cagtgataaa ttttccctct ttgaaatggc cagcggcact tatgctgtca aagaaaaact
     4441 cgcatcggta aaatcttctt taacgcgaat tggtgagaag gtatcccgct taaaagaacg
     4501 ggagaatgaa ctcaatcttc agattgaaaa agtttccgaa catgctcggc aggaagaaaa
     4561 atatctttct gaggaaaagt tgtttattgc acttcgtaaa gaatatttta atctccttat
     4621 tcagcagcgg ttaaaaaaga taaaaggatt agagagtgaa aaagaagaag ttgaaaaaga
     4681 gttaaaacat ttgggggaag aaggagtagg cctggatctt ttattcaata aacaacagga
     4741 attacttcaa gaaaaggaaa aactaaaatc acagatcctg gagcgattag aaagccttcg
     4801 agaagaacta cgctcccttg aacaacagat ttattttcat ctcactgaag cacgacaaag
     4861 agaaaaaaat aagttattaa atcgagaagc cttaaatgag ttagaaccaa aattagccct
     4921 tctacggaag aatgttgaag atctccgcaa taatccttta tatcacgaga ccttagatga
     4981 gttagaagaa aaagaaaaag aattccagaa aaaggtttca gaatttagaa ttcgtaagga
     5041 ttcttataat gaacaaatta accaactcaa acaggaatgg tctcgacttg aaacttcaat
     5101 tgaatatctt actgaagaat tagcatctat cgatcgggaa caaagttctc tcgatttttc
     5161 atcaaccgag ctagttcagc aaatcgagag tttacaaata cagctgtcaa cccttcagga
     5221 agaaagccga aaatttatca aaaagagaga gcagctcaaa gagaagctgg acaggaagaa
     5281 agccttgttg gttaaaattc gaaatggtct ccgtgaatat gaaccagagg aaaaaatcga
     5341 ccaaagaata gccgacctgt ccaaaaatct cattcaaaag ggttggtcgg ggaaagtcgt
     5401 ttatgcattc aattggcttt ttaaagatta tactgctatt ctaagaactg aagggaatct
     5461 gaatttgaaa gactctttac caaagactgg tcagggtttt gtctatttgg atattttgcc
     5521 accatcgaat tattggaaaa ttttcaccaa gaaacaaatc atcgatgcat tgaaaaaccg
     5581 ggaagttcca caggtaaata tgatcagttc cgatggaaat attgtttatc gtagagatgg
     5641 agtgttgata tttccaagga aacttataac caatcagagt ggagttcgct tttatcgaag
     5701 ttggcaaaaa aggggagccg cccttcaaaa aagaatacaa gaaggagaaa aagatatttc
     5761 aggttatctt gtcaaagaaa atcaactgga aaaagagtta accaaaatag aaggagagtt
     5821 gcaagtttta cagcttcgat atgacgactt gcaaaaggaa aaaaagaaga aaattgaaaa
     5881 aattgaactc attaatttaa aaaagcaatc tttcttgagc gaaaaagaaa tccttggcga
     5941 gaaactggat cggttaggat gggaaaaaga agacatagat aacgaagtca atcaattgga
     6001 agatgaatta agagaaattc acaaccaacg atttgagagg gaaaagctcc gatcaaccga
     6061 agataagtta cagggagaac tcaccaccat cgagatgctc attgagaaat cggttttttc
     6121 tcttcaaaat attgagaaca gccgcaatca atccctggat atctggaagg agattatttt
     6181 taaaataaag aaattgaatg aagaacatca atttgaactg aatagaaaaa aagtatcctt
     6241 tgcggcagtg gatgagattc gagaaaggtg taacgatatt gaactgcaaa gagagaagcg
     6301 gaaggatcgg caagattcgc tttcttacaa aagggaaaag ctgtatttac aaaaagaaaa
     6361 atgggatttt gaaattcaag aatataccac tcgcttagat gaataccagg aatggcagac
     6421 cgacgatctc cctttgccct cttttactga tattcatcac ttagagcaga tgataaccga
     6481 aaaagagaac cgtttgaaaa cttgggaaat acgaagaggt tctatttcac aacttcgtga
     6541 tctaaaagaa cgtcaaaatt acttaaagga aaaattgact ttttttcagg atgccatggc
     6601 tcgatttgaa aagaatcgaa tagagtgcga actcttgtgt caagaattgt ttaatgagtt
     6661 tttattagag gtcaatttcc actttcagaa aaattttcaa cgagttttta acggtggcag
     6721 agcaaagatc gaaattgaag aacaaaattt ggaaatagtt atccaaatac cgggaaaaaa
     6781 gaaacaaagg ttgtcgcttt tgtccagcgg agaaaaagcc cttactgccc tttgtctatt
     6841 tttcgctcta tttaaatccg gaggttatcg tttttgtttt tttgatgaag tagatgcgac
     6901 ccttgaccat ttcaacagtg tccggttagc ggaccttatg aaagagtttt ctcatgagtg
     6961 ccaaatcatt attgtaaccc atcaagaaga aatcatggaa gcgtctgacc ggatcattgg
     7021 agtgactatg gataaaccgg ggatttcaag ggtagttact ctttcaggga aaaatttatc
     7081 acttttatct tctcaaaatt aaccaaccat gatttaaatt taaacctatc accaatcgct
     7141 atctttacta aatggagagg tatcacatgg aagaaaagaa aggttttttt cataagtggt
     7201 tcaaaggtgt tgaaaaaatt aaagaaggcg taaaacagca attttcccgt ttatggtcag
     7261 ccgattccct ccaagacgaa gattgggaag ccctggaaga aatgttggtg caagccgatt
     7321 taggaccaaa tttagccatg gagctggtcg aagaatttca ggaaataaaa aaacaaaagg
     7381 cagaaaatgg gaactggcag gattggcttt atgggaaatt gggaagcatt ttggatggaa
     7441 atgattcaag cctattttca tcagctcaaa gtggggcaat gaaagcaatt ctattagcag
     7501 ggattaacgg cgtaggaaaa acaaccttgg ctggcaaatt ggcgcgatac tattcgctgc
     7561 agggtgaaga agtcgccttg gttgcagctg atacttttcg tgctgcggca gttgatcaat
     7621 tggaaatctg ggcggaaagg gctggttgtt tcttttttaa aggttctcct ggagccgacc
     7681 caggatcgat tatatttgat ggaatagaag cagcacgaaa aaagaaccga ggttttgtga
     7741 taattgatac ggctggtcgg tcgcatgtca acaagaatct tttggcagaa ttagaaaaaa
     7801 tgggaaaaat tgtccaaaag aatttctctc ctgaaaatat tgaaaattta ctagttattg
     7861 atgccatggc cggccaaaat gcctttcttc aagccgaatc gattggtaag gctatccccc
     7921 tgaatggagt tattctttcg aagtgggata gtcaggctaa aggaggtatc gttttccgta
     7981 taaaaaaaga attagaattg tcgattaaat ttttaggaat aggtgaaaaa atcgaagatc
     8041 ttcaaccttt tgattctgat caatttgtaa aagcaatagt ttatgacgaa gaagtttctt
     8101 gacagtcgga gcagtgacga ttattatctt atttgtcttt caaaattaaa taaatccagt
     8161 tttggagtca atgcatgttt gaacaattaa ttaataaatt ttctggaata tttaaaaagt
     8221 taagaagcaa aggaaaactg agcgaacaag atgttgattc aatattaaaa gaattacgaa
     8281 tggttctctt agaatccgat gtccattacc gagtggttaa agacctcatc gctcgagtga
     8341 aagaacgagc cgtgggaaga gaagtattag agagtgttac accaggggaa atggtcatta
     8401 agattttatg ggatgaaatc cgaaaggtat taggtggtaa tgtcagttcc ttagagactg
     8461 gtggtgcgac accggttctc attatgttag ttggactcca aggatcggga aagacaacca
     8521 ctcttgggaa attggcggtt cgtttaaaaa atcaaggact ttttcctttg gcaatttcaa
     8581 ctgatactcg ccgtcccgct gctaaagagc agttagcgat tctgtgtcac aattcgggga
     8641 ttgattttta tgacgattcc aaagccttga ctccattaca aatgattcaa aatgctattc
     8701 gtcattcacg ggaaaaaagt tatgacgtga tctttatcga cactgccgga aggcttcatg
     8761 tcgaagaaga gcttttagcc gaactcaaag aggttgttga tacaataccg ttgaaagaaa
     8821 ttttgctggt tttagatgcg acaaccggtc aggaagcggt taaggttgct caatctttta
     8881 aagaatgggt caatcctacc gggctcattt tagcaaaggt cgataccgat gcccggggtg
     8941 gagccgtatt gtccatagtt gctcaaactg gatggccggt taaatttgct ggagtgggcg
     9001 aaaaagttga tcagctggaa gtttttcatc ccgacagaat ggcaagtcgt atcatgggga
     9061 tgggtgatac tcttaccctc attgaaaaga tagaaaggtc gattgacgaa gaagaaaagg
     9121 agaaaataga aaagaagttc ggaaagaaag aactggattt aaatgatttt atggaagaat
     9181 taaaaagagt aaaagaaatt ggctcctttg atgaaattgt tggaatgtta cctggtaatc
     9241 tgcgaggtgg aatttctcag attgatggag agaaaaatat taagcgagtt caggctatta
     9301 ttcagtcaat gacaccagcc gaaagaatca atccatccct tattaattcc agccggcggc
     9361 gaagagtagc tcaaggaagc ggaacaacca ttcaagacgt taaccgttta ttaaaacagt
     9421 ttgaagattt tcgtaaactt tggaaacaga tgaaccgtac ggggaaaaat cccttttcca
     9481 aaagaaaagg atggttcggg tggaacgcat aaataactta atcatctcat ttcgatttca
     9541 tccagtagaa aatatttgat ataaaaagaa aggagaataa cgatggcagt gaaattaaga
     9601 ctcaccaaaa ttgggcgtaa gcatgctccc tattaccgga tagttgcgat cgattcacaa
     9661 aaagcacgag atggaaaacc aattgaaata ttggggaact attcaccgat agtcaacccg
     9721 ccactttggt caatcaattt cgaccggatt cagtactggc ttaacaatgg agcaacaatg
     9781 tctgacaagg ttaagacttt gttaaaacag acggaaaggc cgggtataga gtgaaggatc
     9841 tcgtagaata catggtcaag tctttagttg acaatccaga acaagttaaa gtctccgagg
     9901 tccaaggtga acaatcggtg atttatgaag tgcgagttgc ccccgaagat atggggaaag
     9961 tcatagggaa acaaggaaga atcgctcgct cgattcggac tatagtgaaa gcagcttgtg
    10021 ttaaaactgg caagaaggcg gttgttgaga tattggaata gaaaagaata agattgtagt
    10081 tggtaaattt attaaacctc atggattagc aggtgggtta aaagcccttc cctttggaga
    10141 aacattaaat agtttttctc ctggaactat tttgtttgtt gaagatcaag accaataccg
    10201 taccctggtc gttaaagact ttcaacccat gggtcggttt gttctattat ggttaaggga
    10261 tttgagtacc aaagctgaag tcgaatcgtt atctggtcaa ttaatctgga gagagaaatc
    10321 ccaggtaagc gtgactgata caccggattc ttattttgtt tatcagctga ttgattcgaa
    10381 aataatggaa aatggccaat cccttggtgt tgtctccgat gttattgaag ggccagccta
    10441 tgactactta caggtgaata gagacaatag ggaattttta atccctttta tccgggttta
    10501 tattaagcat gttgaccttc aggggggata tataaccgtc gactgtccaa agggtttttg
    10561 ggaatgaagt tacggattga tatcattacc ttgtttcctg aactgattcg ttgttttgcc
    10621 gaaactgcga ttgtaaaaag agctcaagat cggggcttag tattcattaa agcttataat
    10681 ttgagagcat tttcttctga ccgatatcgt actgttgacg attatcccta tggtggcgga
    10741 ccaggaatgg tgctcaaacc ggaaccgatc tcccgagcgg ttgaatttat taaaggagtc
    10801 actcattccc aaccgagagt tttgattacc agcccccaag gaagagtttt taaccagtcc
    10861 ttagcagaag ctctttctct ttgccctcat attattctgg tttgtggacg ctatcagggg
    10921 attgatcaaa gggttattga actctgccaa ggagaagaaa tatcggttgg tgattatgtt
    10981 ctatcaggcg gtgaattgcc ggcaatgatc gttgccgaag caataactcg attagttccc
    11041 gaggttcttg gtgatgaaga atcgattcat tttgattcct tttacgatag gatattggga
    11101 ccaccgcaat atactcgacc tcggattttt gaaggaagag cagttccgga ggttttaacc
    11161 agtgggaatc atagtttgat tgaagagtgg aggcgaaaga aagcacttca aaaaacccgg
    11221 ttagttcgtc cggatttact ggataaattg agtgatgaag tgattcatga atagtaacgg
    11281 ttgtcaagcg ttaggcagca tggtatagta ttattcggtt ttttgagaaa aatttattta
    11341 ttagtcgata aatcgattga gaaaatgatt gcaccacttc gatagaatga acagtgggtt
    11401 ttcaggagga ggtccataat ggataaggca aggttgttac attcggtaga atcagcttat
    11461 ataaaagaaa ttcctgatgt tgagccaggg caaacggtaa gagtacattt aaaagtcgta
    11521 gaaggagaca aagagcgtat tcaggttttc gaaggattgg ttattgcact gaaaggttct
    11581 ggaatcagcc gaaccatgac ggttcggaaa atgtcctttg gtataggaat agaaagagta
    11641 ttcccaatct attcgccacg tattgaaacc atcgaaatag tccgtcgagg caaagtgcgg
    11701 agagcaaaac tttattatct ccgtgggaaa agtgccaaag aatcaagaat tgaggaaaaa
    11761 cgcgagaggt aattcgataa acgtcttgag ggaaatagtt gaaaccgttt tttgggcact
    11821 cctgattgca tttattgttc ggtattttgt ggtagaaggg tactatatcc cctctagttc
    11881 aatgaaaccg actctgatac caggtaatag agttctcgta gcgaaatttt attatcgctt
    11941 ttaccgtcct caccgtggtg atattattgt tttccgtttt ccctccaata ggaagaagaa
    12001 tttaataaaa agagtaattg ctcttcctgg agaaacggtc cgaatagaaa acggtttggt
    12061 acatatcgat ggtattcctc ttcagggaga tcaatttaat cgagagtatt ttaacgttgg
    12121 gaattatgga agagaagaac aagtgatacc ggaaaattcc tattttgttt taggagataa
    12181 tagtcataat agtgatgata gtcgattttg ggggtatgtt ccatttaaaa acattttagg
    12241 acgtgctttt ttggtttatt ggcctccacg gtcaattcaa atattgcgtt gaaaatgttg
    12301 gaaaaagacc tttgggtaaa aggctttaaa tatgttggtg gagtcgatga agcagggaga
    12361 ggaccattgg ctggtcctct cgtatcgtgt tgtgttattt ttcctccggg atgtattcct
    12421 tctggtttaa atgattccaa acttcttagt cctgcccaga gaaaccattg ttttcaactt
    12481 atttgcagaa ctgcttttag cctcggggta agtatcgttg gggagaaaat gattgattgt
    12541 ctcggaatac aaagcgccaa tctttattcc tttcgcgaag ccttgatgcg agcctcggta
    12601 aaaagaactc ccgaatttgt tatttttgat tggttgaaga tacctgaatt ggatattccc
    12661 tcgatttcaa tctcgcatgc ggaactaaaa agcttttcgg ttgccgctgc ttcaattgtg
    12721 gcaaaggtag ttcgtgattc tcttatggaa aaacaatatc aagaacttta tcctgagtat
    12781 ggatttatcc atcataaggg atacggtact aaacttcaca gggacaaaat aaatcaactt
    12841 ggcatagcga attgtcatag aaggagtttt tgtggtaaat acctctaaaa gaattggtca
    12901 gatgggtgaa gaaattgcct ctcgaattgt tattcgtcaa ggcaaaatga aaattatcga
    12961 aagaaatttt cgatctcgaa taggagaaat cgatattatt gctctggata aaaatacctt
    13021 agtcttcatc gaagtaaaaa gccgggcggt cttaaacgca ggtttccccg aggagtcggt
    13081 aactaaatac aagcaggaca aaattcgaaa agttgctctt tattatctca tttctcaggg
    13141 atataatccc aaccatacaa attaccggtt cgatgttatt tctattacct ttaatggaga
    13201 cgactatgct catccgagtg ttaaatatta tataaacgct ttctaatcag tacagatttg
    13261 attaattgta actcttttca aaatgttgtg aataggtaga gggcagtgta ttatgctatg
    13321 cccaaccaaa accatttact acca
//
LOCUS       DAOSOL010000003        11656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_5494_length_11656_cov_4.489096, whole genome shotgun sequence.
ACCESSION   DAOSOL010000003 DAOSOL010000000
VERSION     DAOSOL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11656
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5494_length_11656_cov_4.489096"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..541
                     /locus_tag="PK171_00180"
     CDS             281..541
                     /locus_tag="PK171_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04652.1"
                     /translation="MGYWAVVEKRENGFWAFFPDIVEVEVRSKTIEEIKVLLKESLAE
                     RLQFLNQTDKHQPVARFNPTVQTTNPKKISPNVFEFLYIKTE"
     gene            complement(553..2322)
                     /locus_tag="PK171_00185"
     CDS             complement(553..2322)
                     /locus_tag="PK171_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04653.1"
                     /translation="MKSFTLRKKEVIIHTEGEICGNIQELTNSPIFERIVHAFMDHLK
                     KHNSLLSDSLFPPEAELSYRSKILSLLRILTTSSLEEVVKILPDYSYFLDKKNLLHEF
                     VEKLYNFWRRYERYMICHSESGIYSHDQKPYRTFNMTVERLSHLVRAGYRDICENITG
                     THPRVYRQVDAGCQAGIIAVPKIWNPPSEEYRELLSIPFIRQLMIQPPLLLDPPMNKR
                     TGHFQKVTQNLLPNIMIDEKKWLCYPAQVGKLVIFIYFEEGFMGLGCSLANLFELADD
                     DQIKEGPDAIYLYGVDPAMFPTDINSETVFFDDEPNQILIGFVPGNDQYGYFGYLKKM
                     VLTLHNVVMMKKGILPFHGAAFRITNLDKSVFTIAIIGDTATGKSETLEALRIMGNGY
                     IADLKIIADDMGSFEIDETNHQILCYGTEIGAFVRLDDLEKGYAFEQIDRAIIMSPQK
                     TNARVILPVTTVDCILKGFPIDYIFYANNHEEIDEFHPVIERFPDIEKALEVFQEGKA
                     MSKGTTTSVGIVNSYFANNFGPIQYFDLHEKIAQRYFSEFFKANIFIGQIRTRLGIPG
                     QEMNGPQTASTALIELVGLKSKK"
     gene            complement(2650..3018)
                     /locus_tag="PK171_00190"
     CDS             complement(2650..3018)
                     /locus_tag="PK171_00190"
                     /inference="COORDINATES: protein motif:HMM:NF021568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HOT04654.1"
                     /translation="MKKNRSDLEIQISSTLVQFEKEYMGRGPVDIKTYLFDDIVFIRL
                     KGVLTKAETQLASGGSKDNRDLIKRVRIELLEKGRPLLEAIIENILNRKVVSLHTDIS
                     TRTGERLIIFTLNEPLNDLF"
     gene            complement(3202..3276)
                     /locus_tag="PK171_00195"
     tRNA            complement(3202..3276)
                     /locus_tag="PK171_00195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3242..3244),aa:Val,seq:cac)
     gene            complement(3280..3368)
                     /locus_tag="PK171_00200"
     tRNA            complement(3280..3368)
                     /locus_tag="PK171_00200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3331..3333),aa:Leu,seq:cag)
     gene            3593..5122
                     /locus_tag="PK171_00205"
     CDS             3593..5122
                     /locus_tag="PK171_00205"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT04655.1"
                     /translation="MDKTINNGDSILQFENVTKRFPGVLALDSVSFDVLKGEVHALVG
                     ENGAGKSTLIKIVTGVYQKTSGSILFQGKLIDYQNPHEALRNGIAAIYQELNLIPALT
                     VAENIFMGHHIRNERGFIDWKKMREEAQKLIDFLEVDVEIDAKVGTLGVAKKQVVEIA
                     KALSLNAQILIMDEPTAALAKKEIETLFRIIKFLKSKGVTIIYISHHLDEIFAISDQV
                     TVLRDGKHVATNNTATLTIDDLIKMMVGRKLVEQFPHMDHQAKDEVLRVENLKRKGVL
                     HNINFSLKKGQIVGIAGMVGSGRTELLRAIYGLDPLDGGKIFVKGDEVNIQSPYDAIN
                     LGIALLPEERKTQGLVLLLSVTDNIGLPSLTKISNRGFIDDTKLNHITSEMVQMMNIK
                     TPSFHQKTMFLSGGNQQKVVLGKWFARECDIYLFDEPTRGIDVGAKIEIYHLMNKLIE
                     RGAAILMVSSELPEILGMSDQVLVMKEGKVTGTLTRQNANQEEVLKLAIGGGHLNGRS
                     D"
     gene            5106..6086
                     /locus_tag="PK171_00210"
     CDS             5106..6086
                     /locus_tag="PK171_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT04656.1"
                     /translation="MDALTEDKNQGLVERFFIFWDKIGILLVFIVVCVIFGILNPVFF
                     NPLNIINVIRQVSIIGVMAVGMTLVILLGLIDLSVGSIVAFAGIIAAGFQVKWGGSLF
                     LSLVIPLLVGAGIGYFNGYVSTKGGIHPFIVTLGSMSIFRGATLLIAQGKPISGMSPA
                     FRFIGAGMIGPIPFPVILFLGCVIIFGIMLKRTVFGRYIYAIGGNQEAALLSGIMVDR
                     VKILTYTILGTLSGLSALILTSRLNSGELVAGQGYELDVIASVVIGGTSMMGGEGGVY
                     GTLIGALLIGVISNGLNLLGVQPYWQMMVKGTIIILAVLMDRMKRRFRTV"
     gene            6232..7191
                     /locus_tag="PK171_00215"
     CDS             6232..7191
                     /locus_tag="PK171_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT04657.1"
                     /translation="MKKIGLLFIVAILAFSCLFGTLAFAQEYTIGLSFPSLSFAWFAF
                     LEQAVKDKASQLGVNVVSLEANDEVSKQISIIEDMVIKKVNGVLLVPIEVEAVVPGVE
                     ALNKANIPVVTVDRRLKEGVPVQVLAHVGADNYTGGKKAAEFIVAELNKKFGESKGTV
                     IELYGTPGAGPAIDRSSGFQDVMKQYPNITVKTQTANFRRDDGMQVMEDFIMSSPEID
                     AVFGANDEMILGALEALQASAKFNVQEVVTIGFDALTDALKSISDGILDATIEQFPGK
                     QAATGFEILYNFVKDGKTPEDSVILIEPAVITKDNLDQAEKSF"
     gene            7321..8322
                     /locus_tag="PK171_00220"
     CDS             7321..8322
                     /locus_tag="PK171_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT04658.1"
                     /translation="MSSMKDVAKIAGVSVSTVSRVINKSIPVNNETRLRVEKAIRKLH
                     YKPNLLAKGLRMRSGGIIGLVVPEIIHQTFVNFIHYVEEQIVDHGFDLILGNTQEDPE
                     KEERFIDSLIRRNVDGIIFSRVSDQSRVFNILEKTNIPVVVVDRFLEKEDIPSIVLNN
                     YQAGILAGSHLISLNHKKIACITGPLNIALCRERLKGFKTILSENGVLLPPDFIYEGD
                     FKFESGIYAIKKFLNDDIKVTAIWAQNDMMAIGALKELNQNHIRVPEDISLIGMDNIS
                     SSEMVTPSLTTIIQPFQEMCQRAVQIILNHIHKKEITLNRIVLEPSLIIRESTRPFK"
     gene            8462..9664
                     /locus_tag="PK171_00225"
     CDS             8462..9664
                     /locus_tag="PK171_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04659.1"
                     /translation="MTNSSKVVEKALEQGKGIFRLFPNWVPRSFCIPGKRLKLHPDDY
                     YAFGAHRGGIDERWFASTTKADNGPETLPDEGLSYIYFKDGRKEEKVLLKEAIDIMGN
                     DILGTDVMKKYGGWLMFSKFFDNMEPLPHHLHHDDEAAANVGAMGKPESYYFPAQLNN
                     HGGWFPYTFFGLTPGTTKDDIRKCLENWNKGDNGILYLSRAYKLKPGTGWDVPPGVLH
                     APGSLLTYEPQRSSDVFAMFQSLVWDVPTPWELLTKNVPEEHKNDLDYIVSLINWDLN
                     VDPDFYQNRFAEPKPVKPVEEMKSEGYEEYWISYKSSNFSAKELRVFPGKTVTIKDEG
                     AYGLITIEGHGQFGSLSIDAPSLIRFGEMTSDELFVTQKAAREGITITNQSENGMLVI
                     LKHFGPEL"
     gene            complement(9739..11154)
                     /locus_tag="PK171_00230"
     CDS             complement(9739..11154)
                     /locus_tag="PK171_00230"
                     /inference="COORDINATES: protein motif:HMM:NF019496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HOT04660.1"
                     /translation="MSKIGVITFSDGRPHVHQELLAMNLKFQENLVKKLQQEGHEVII
                     ADEPPWTNQLAVSAGKKMQLAGVDCTIFNYAIWAWPHFTVLAAQFAPGPFLCLSNINP
                     GYPGLVGMMASSGALDNVGIPYTRVSGDLNQESTFKKVNCFIKAATCLKQIRGETFGC
                     FGGRPMGMYTATSGTDSWMKVFGIDTEHIDQWEIVRRSEFLLAQNPKKIEQAFQWCEK
                     NIGSIQYDGKQLTPEILKKQIASYYAVKELCQEMHLDFCGIKGQPELTNNFCTMDVAE
                     AFMNDPYDFDGPKEPIVCSTEADMDAALTMEIFKKLAKTPVLFADVRHWHDDYKILDL
                     CNSGEHATYFAGASFDYRDNLPRVSLLPESFYFQAGGAAVHHLAHPGKVTLARLGRKN
                     GNYWMAILTGEFLQFDKKTNQEIMARTQAEWPHAFCKLDTSIERFIEKFPCNHIHGVY
                     GHYVEELKMVCEFLGIQWEII"
BASE COUNT         3664 a         1976 c         2403 g         3613 t
ORIGIN      
        1 cactccagat aaaggtatca acaccaaatt tgaacattgt aaagcaacct cctttagccg
       61 catcaaggac attttttaat tattgtacca ttaaatataa aagaattagg gaataaatgt
      121 aagctattga taccaaaaat taaaggagct tcattaccaa gattaaattt ttgtataata
      181 aatagaacca tgaatttacc aggaaagtga tctatcatta accagtggaa aaatttcatt
      241 ggtaaaagtt acctttttta tttattggag gggatgaaaa atgggatatt gggcggtggt
      301 agaaaaaaga gagaatggat tttgggcttt tttccctgat attgttgaag tcgaggttcg
      361 atcaaaaact atagaagaaa taaaagttct tctcaaagaa tcattagccg aacgcctaca
      421 attcttaaac caaaccgata aacatcagcc agttgcacgt tttaatccca ctgttcaaac
      481 taccaatcct aaaaaaattt cacccaatgt ttttgagttt ttatacatca aaaccgaata
      541 atttctctta atttatttct tactttttaa accaactaat tcaataagag cagtggaagc
      601 ggtttgaggt ccattcattt cttgaccggg aattcctaat cgggttcgaa tttggccgat
      661 gaagatattg gctttaaaaa attcagaaaa ataacgttgg gcaatttttt catggagatc
      721 aaaatattgt ataggcccaa aattatttgc gaaataagaa ttcacaattc ctactgaagt
      781 ggtggttcct tttgacattg ccttcccttc ctggaatact tccaaagctt tttcaatgtc
      841 tggaaagcgt tctataactg gatgaaactc atcaatttct tcgtggttat tcgcatagaa
      901 aatataatcg attgggaatc cctttaaaat acaatctaca gttgttactg gtaaaataac
      961 tcgggcgttg gttttttgag gactcatgat gatagcccga tcaatttgct caaatgcata
     1021 gcctttttct aagtcatcaa gtcgaacaaa ggcaccaatc tcggtcccat aacaaagaat
     1081 ttgatgattt gtttcgtcaa tctcaaatga tcccatatca tcagcaataa tttttagatc
     1141 agcaatatat ccgttaccca tgattcggag agcttccagg gtttctgatt tacccgtagc
     1201 cgtatctcca ataatagcaa ttgtaaagac cgatttatcc aaattggtga tacggaaagc
     1261 cgcaccatgg aaaggcaaga ttcctttttt catcatgaca acattatgga gggtaagaac
     1321 catcttttta agatagccaa aatacccgta ttggtcattt ccaggaacga agccgatcag
     1381 aatctgattc ggttcatcat caaaaaagac tgtttcagaa ttgatatcag tagggaacat
     1441 tgcaggatca accccgtata ggtaaatggc atctggtcct tctttgattt ggtcgtcatc
     1501 ggctagttca aaaagattgg ccaaagagca acccaaaccc ataaaacctt cttcaaaata
     1561 gataaagata accaatttcc caacttgagc cgggtaacaa agccattttt tttcatcgat
     1621 cataatattg ggtaacaggt tttgagtaac tttttggaaa tgcccggttc gcttattcat
     1681 tgggggatcg aggagcaagg gaggttgtat cattagttgc cgaataaaag ggatcgaaag
     1741 gagttccctg tattcttcgg aaggaggatt ccaaattttt gggaccgcaa taattccggc
     1801 ttggcagccg gcgtcaactt gacgataaac tcgtggatga gtaccagtga tgttctcaca
     1861 tatatcacga tatccagcac gaactaagtg ggaaagacgt tcaactgtca tgttaaaagt
     1921 tcgatatggc ttttggtcat gggaatagat tcccgattca gaatgacaaa tcatatatct
     1981 ttcatagcgt cgccagaaat tatagagctt ttccacaaat tcatggagta aatttttctt
     2041 atctaaaaag taactataat caggaagtat tttaaccacc tcttcgagag atgatgtagt
     2101 taaaatacgc agtagggata gtatttttga acgataggaa agctcagctt ctggaggaaa
     2161 caaagaatca ctcaacaagg aattgtgctt ttttagatgg tccatgaagg cgtgaactat
     2221 tctttcgaaa atcgggctat tagttaattc ttggatgttt ccacaaattt ctccttcagt
     2281 atggatgatc acttctttct tcctaagggt gaagcttttc attttactgc tccttttttt
     2341 aaaataaaaa aggactggag ttatagctta accccagccc tttccaaatc gtcattaata
     2401 ctctttggct tccggctgta tcattcttta tgtgaaacac caatactctt aatgttccac
     2461 cgatttaagt ggttatttta ctcattctga taatgactgt caatgtttat gaaaaaaaag
     2521 gaaaataacg aggaggtgca aaaattaata tttttaattt tcttgaaaaa gggaaaagtt
     2581 gaattggtaa aaaattagac tcaggttgtg gggatttaca ggaggagata taaattcacc
     2641 aagttaattt taaaacagat cattcagtgg ttcatttaag gtaaagatta tcaaccgctc
     2701 accagtacgg gtgctaatat cggtatgtaa gctcactact tttcgattca ggatattttc
     2761 aataatagct tccaaaagtg gtctcccttt ttctaaaagc tcaatgcgga cccgttttat
     2821 taaatcgcgg ttgtccttac tcccgcccga agctaattgt gtttcagctt tggtgagcac
     2881 acctttcaaa cgtataaaaa cgatatcatc aaaaagatag gtctttatat caaccggacc
     2941 tcttcccatg tattcttttt caaactggac aagagtcgag cttatttgaa tttccaaatc
     3001 gcttctattt tttttcatcc tgaattccat cttaaaggta tattaggaaa taagactaat
     3061 gataattcaa acgaatgtaa aattatccaa taacttctta atttttagaa attgaactga
     3121 atggccggca acaaataagt taattgaaag aaaagactta ttttaaaata aatcaaaccg
     3181 aggattttac tttaaaaaaa atggtgcgcg atgctgggtt cgaaccagcg acttccaccg
     3241 tgtgaggatg gcactctacc gctgagttaa tcgcgcatct ggtgccgaag gggggatttg
     3301 aacccccacg agcgttaaac tcactggaac ctgaatccag cgcgtctgcc aagttccgcc
     3361 acttcggcga agggcaaaat ataaaatagc gtatttcata agccttgtca agaactgaaa
     3421 aagatgataa aaatttgaga aacaatccaa tttattttat ttgaaagtaa aagtcaaaat
     3481 gaaattgata aaaataagaa gagaatatag aatagtaaaa agaatatttt tttgatttta
     3541 ctttgaaaac gatttcaaaa acttaaagga gtatttatag tggagaaaga atatggataa
     3601 gacaatcaat aatggcgaca gtattttaca gtttgaaaat gtaactaaac gctttccagg
     3661 tgtattggct cttgacagtg ttagtttcga tgtattaaag ggagaagttc atgctttagt
     3721 tggagaaaat ggggcgggga agtcaacttt aataaaaatt gttaccggtg tttaccaaaa
     3781 aacatccgga agtattcttt ttcaggggaa actaattgat taccaaaatc ctcatgaagc
     3841 acttcgaaat ggaattgcag ctatttatca agaattgaat ttaatacctg ccttgacggt
     3901 cgctgagaac atctttatgg gtcaccacat tcgcaacgag cgaggattta ttgattggaa
     3961 aaagatgaga gaagaagccc aaaagctcat tgattttctt gaagtggatg ttgagattga
     4021 tgctaaagtt ggaactttag gtgtggcaaa aaaacaggtg gttgagattg caaaagcact
     4081 ttctctcaac gctcaaatat taataatgga tgagccaacg gcagcattgg ctaaaaaaga
     4141 aattgaaaca ctatttcgca ttatcaagtt cctgaaatcg aagggagtaa ccattattta
     4201 tatttctcac catctcgacg aaatatttgc tatctccgat caagtgacag ttttaagaga
     4261 tggaaagcat gttgcaacca acaatactgc tacgttaacg attgatgatt tgataaaaat
     4321 gatggtgggc cggaagttag ttgaacaatt cccccatatg gatcatcagg ctaaggatga
     4381 ggttttacga gtagagaatt taaaacgaaa aggagtgttg cacaatatta atttttccct
     4441 caaaaaagga caaatcgtag gaattgctgg aatggttggt tcgggccgga ctgaactctt
     4501 gcgtgccata tatggcttag atcctttaga tggtggaaag atttttgtga aaggagatga
     4561 agttaacatt cagtctccct atgatgccat taatttgggt attgcacttc ttcctgaaga
     4621 aagaaaaacc caaggtttgg tattgttatt atcggtcacg gataacattg gattgccttc
     4681 tttaaccaag atatccaatc gcggttttat tgatgatacg aagctcaatc atatcacttc
     4741 tgaaatggta caaatgatga atattaaaac tccttccttt catcaaaaaa ccatgtttct
     4801 ttccgggggg aatcaacaaa aagtggtatt gggtaaatgg tttgctcggg aatgcgatat
     4861 ttacctattt gatgaaccaa cccggggtat tgatgttgga gctaagattg aaatttatca
     4921 cttaatgaac aaacttattg aacgaggggc tgcaatcttg atggtttctt cagaattacc
     4981 agaaatttta gggatgagcg atcaggtatt ggttatgaaa gaagggaagg tcactgggac
     5041 tttaacccgc caaaatgcga accaagaaga agttttaaaa ctggcaatag gaggggggca
     5101 tttaaatgga cgctctgact gaagataaaa atcagggatt agttgaacgt ttttttattt
     5161 tttgggataa gattgggatc ctcttagtgt ttattgtggt ctgtgtaatt tttggaattc
     5221 ttaatccagt gttttttaat cccctcaata tcatcaatgt tattcgacag gtttcaatca
     5281 tcggtgttat ggcagttggc atgacattag ttattttatt gggcctcatt gatctatcgg
     5341 tgggttctat tgttgctttt gccgggatta ttgctgccgg ctttcaagta aaatggggcg
     5401 gaagtttatt tcttagctta gttattcctt tacttgtagg agctggtatt gggtatttta
     5461 atggatatgt ttccactaaa ggtggaatac atccctttat tgttactttg gggtcaatga
     5521 gtattttccg tggggcaaca ctactcatcg ctcaggggaa accaatttct gggatgagtc
     5581 ccgctttccg ttttattgga gctgggatga ttggtcctat tcctttcccg gtcattcttt
     5641 ttttaggatg tgttatcatt tttggcatta tgcttaagag aacggttttc ggccgttata
     5701 tttacgcaat cggagggaat caagaggcag ctttgctttc gggtattatg gttgatcgag
     5761 taaaaattct cacctatacc attcttggca ccctctcagg gttaagtgcg cttatcctca
     5821 cgtcgaggct caattctgga gaactggtag cagggcaggg ttatgaatta gatgttattg
     5881 cctcagtggt tatcggtgga acgagtatga tgggtggcga agggggagtt tatggtaccc
     5941 taattggagc attgctcatt ggagttattt ctaacggttt aaacttatta ggagtccaac
     6001 cctattggca gatgatggtc aagggaacaa ttattattct tgcggtcctt atggatagaa
     6061 tgaaacgccg tttccggact gtttaatcaa taaactttca aaaccgatgc acttttttaa
     6121 caactatgaa aggagataaa ttttcggtac cagtttagta attaagtggg ggtgataaaa
     6181 aaagaaaagt taggtttttt attttttaaa aaaactaaga ggaggataaa aatgaaaaaa
     6241 attggattat tgttcattgt agcaatcttg gcttttagtt gtttgtttgg aactttagct
     6301 tttgctcaag aatataccat cgggctttca ttcccatctc tttcctttgc ctggtttgct
     6361 tttctcgagc aagcagtgaa agataaggcc agccaattgg gagtgaatgt tgtatcattg
     6421 gaagccaacg atgaagtttc aaaacaaata tcaatcattg aagatatggt tattaaaaaa
     6481 gtgaatggag tattattggt tccaatcgaa gtcgaagcag ttgttcctgg tgttgaagca
     6541 ctcaataaag ctaatattcc cgtggtgacc gtggatcgca gattgaaaga gggagttcct
     6601 gtacaggttc ttgcccatgt gggcgcagac aactatactg gtggaaagaa agccgccgaa
     6661 tttattgttg ccgaattgaa taaaaaattt ggcgaatcca aaggaaccgt gattgagctt
     6721 tatggtactc caggtgctgg tccggcaatt gatcgtagtt ctggattcca ggatgttatg
     6781 aagcaatatc caaatataac agtaaagact cagactgcta atttccgtcg agatgatggc
     6841 atgcaagtta tggaagattt tattatgagc agtccggaaa ttgatgcagt atttggagca
     6901 aacgatgaaa tgattctcgg cgctcttgaa gctttacaag ccagtgccaa attcaacgtc
     6961 caagaagtgg ttacgattgg attcgacgca ctaaccgatg cactgaaatc tattagtgat
     7021 ggaattcttg atgcaaccat tgagcaattt cctgggaaac aagcggcaac cggttttgaa
     7081 attctttata atttcgtaaa agatggtaaa accccggaag attcggttat tttaattgaa
     7141 ccagccgtaa tcactaaaga taatcttgat caagcagaaa aaagctttta gatgaagcat
     7201 ttcctcatag gggagctgta aaacttatgg cctcccctat gaggagaggg tttatataat
     7261 taaataattg aaatgtaaaa ccaatttcta ataagaaagt caaaaattag gtcatcttct
     7321 atgtcatcga tgaaagatgt agcaaaaatt gctggagttt cggtttcaac tgtttccagg
     7381 gttattaata aaagcattcc ggtaaacaat gaaactcgct tgagagtgga aaaggcgatt
     7441 cgcaagctcc actacaaacc aaaccttctt gctaagggtc ttcgtatgcg gagtggaggt
     7501 atcattggtt tggtcgttcc tgaaattatc catcaaactt ttgtcaattt tattcattat
     7561 gtagaggaac aaattgttga tcacggattc gatttgattt taggaaatac tcaagaagat
     7621 ccagaaaaag aagagcggtt tattgatagt ttgattcgaa gaaatgtcga tggaattatt
     7681 ttttctcgag tttctgatca gagccgggtt tttaatattc ttgagaaaac caatattccg
     7741 gttgtggttg ttgatcgttt tttagaaaag gaagatattc ccagtattgt tctgaataac
     7801 tatcaagccg ggattttggc tggttctcat ttgatcagtt taaaccataa aaaaattgct
     7861 tgtattactg gtccattaaa tattgctctt tgtcgagagc gattaaaagg ctttaaaact
     7921 attctttctg aaaatggagt tcttttaccc ccggatttta tctatgaagg ggacttcaaa
     7981 tttgaatcgg ggatttacgc tattaagaaa tttttaaatg atgatattaa agttacggca
     8041 atttgggcac aaaatgatat gatggcaatt ggagccctca aagagctcaa ccaaaatcac
     8101 atccgtgtac ctgaagatat ttccttaatt gggatggata atatcagctc ttcggaaatg
     8161 gttactccat cgctaaccac cattatccaa ccatttcagg aaatgtgtca aagggcagtt
     8221 caaattatct tgaatcatat acacaagaaa gaaattactc tcaatcggat tgttttggaa
     8281 ccaagcttaa taatccgtga atcaactcgt cctttcaaat aattataata ggtgggctgc
     8341 gatcagttaa tgaggattaa attggggaaa aatgattatc cccatggaat aaggatatga
     8401 ttttatggta aatattttga tgataatcaa aaaaaggaaa ccatagaaaa gagggaatgt
     8461 tatgacgaat tcatcaaaag ttgtagaaaa agctttggag caagggaagg gaatctttcg
     8521 attatttccc aattgggttc ctcgctcatt ttgtattcca ggtaagcgtt taaaacttca
     8581 ccctgatgat tactatgctt ttggagctca tcgaggcggt attgacgaac gttggtttgc
     8641 ttcgacaacc aaagcagata atggaccaga aacgcttccg gatgagggtt taagctatat
     8701 ttattttaaa gatggacgta aagaagaaaa agttcttctt aaagaagcaa ttgatatcat
     8761 ggggaatgat atcttaggga ccgatgttat gaagaaatat ggtggctggt taatgtttag
     8821 taagtttttt gataatatgg aacccctccc tcatcatctt catcatgatg atgaggcagc
     8881 cgctaatgtt ggagcgatgg ggaaaccaga gtcatactat tttccagccc agctgaataa
     8941 ccacggtggc tggttccctt atactttttt cggtcttact cctggaacca ctaaggatga
     9001 tataaggaaa tgtttagaaa attggaataa aggtgataat ggaattttgt atttgtctcg
     9061 ggcatataaa cttaaacctg gaactgggtg ggatgtcccc cctggtgttc ttcatgcgcc
     9121 tggctctttg ttaacctatg aaccccagag aagttcggat gtttttgcta tgttccaatc
     9181 attggtatgg gacgttccaa caccctggga gttattgaca aaaaatgttc ccgaagagca
     9241 taagaatgat cttgattata tcgttagctt gataaattgg gatttgaatg ttgatccaga
     9301 tttttatcaa aaccgatttg ccgaacccaa gccagtcaaa ccagtggaag aaatgaaatc
     9361 cgaaggttat gaggaatatt ggatatcgta taaatccagc aatttctcag ctaaagagtt
     9421 aagagtgttt cccggaaaaa cggttaccat caaagatgaa ggggcttatg gtttaattac
     9481 cattgagggt catggtcagt ttggatcgtt atctatcgat gctccaagct taattcgttt
     9541 tggagaaatg accagcgacg aattgtttgt aacccaaaag gctgcccggg aaggaataac
     9601 cattaccaat caaagtgaaa atggtatgct ggtaatacta aaacactttg ggccggaatt
     9661 gtaaggttgg tatcaatttt taaaaaagag cgggattttt tattgaaccc gctctttttt
     9721 aaaaaatata gatttgagct aaatgatttc ccattgaata ccaagaaact cacaaaccat
     9781 tttgagctct tcaacataat gaccatatac tccatggatg tggttgcagg gaaacttttc
     9841 aataaaacgc tcaatactcg tatcaagttt acaaaatgca tgaggccatt cagcttgagt
     9901 tcgagccatg atttcttggt tggttttttt atcaaactgg agaaactccc cagttaaaat
     9961 ggccatccag taatttccat tttttcttcc taatcgagcc agggtgactt ttcctggatg
    10021 ggcaagatgg tgaaccgcag caccaccagc ttggaaatag aaactttcag gaagcaatga
    10081 aactcgagga agattatcgc gataatcgaa gctggctcca gcaaagtagg tggcgtgctc
    10141 accagagtta cacaagtcca gaattttata atcatcatgc caatgacgaa catcggcaaa
    10201 tagaaccggg gttttggcca attttttaaa gatctccatg gttaaagctg cgtccatatc
    10261 tgcttcggta gagcatacta taggttcttt gggtccatcg aaatcatagg ggtcattcat
    10321 aaaagcttca gcgacatcca tagtacaaaa attattagtg agctcaggtt gacccttgat
    10381 tccacaaaaa tctaaatgca tttcttgaca taattcttta actgcataat aggaagcgat
    10441 ttgctttttt aaaatttctg gggttaattg tttcccatca tattgaatcg agccgatatt
    10501 tttttcacac cattggaagg cttgttcaat ttttttcggg ttttgagcca gcaagaattc
    10561 tgaacgccga actatttccc attgatcgat atgttcggta tcaattccga atactttcat
    10621 ccaagaatcg gttccggagg tagcagtata cattcccatt ggacgtcctc caaaacaccc
    10681 aaaggtttca ccacgaattt gttttaaaca ggtagcagct ttgataaaac agtttacctt
    10741 tttaaaggtt gattcctggt ttaaatcacc tgaaacccgg gtataaggaa tgccaacatt
    10801 gtccaatgca ccagagctgg ccatcatacc gactaaacct ggatatccag gattaatatt
    10861 tgataggcag agaaagggac caggagcaaa ctgagctgct aaaacggtaa aatgaggcca
    10921 agcccaaatg gcataattaa aaattgtaca atcaactcca gcaagctgca tttttttccc
    10981 ggctgaaact gccagctgat tggtccaagg aggctcatcg gcaataatga cttcatggcc
    11041 ttcttgttga agttttttta ccaagttctc ctggaatttt aaattcattg ctaataattc
    11101 ctggtgaaca tgagggcgac catcggaaaa agtaatgaca ccaattttac tcataactat
    11161 tcctccttta ttcaagatgt aaaaagcaac cgatttcatc aatattatcg cttttacctc
    11221 ctaaatgtca aatttcaaac tcttataata aaaaaaggtt tttaataaaa ttctctatca
    11281 caaggttaaa atgccttttt taaagagtta aatctcaaat atttttaaat gatatatata
    11341 taattgaacc acggtttaga aggcgttttg gacttgttga caattaatgc agggcaatga
    11401 taagatgaaa tcggttacaa tttttagaaa ggtggtgata ggagttttat tcagagattg
    11461 gtctttaaaa ggttaggaaa gatttataat ttgtataagg gagggaaaaa ccttgaagag
    11521 gttacttttt gtggggatgg ttttactttt ggttttgtcg accagtacag tattcggttt
    11581 tgaattagga cgatttgatg ggacgacgat taaggcttgt tttattggtg ggggagacta
    11641 cgaaaaaatt tacgag
//
LOCUS       DAOSOL010000004        11387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_5677_length_11387_cov_4.069802, whole genome shotgun sequence.
ACCESSION   DAOSOL010000004 DAOSOL010000000
VERSION     DAOSOL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5677_length_11387_cov_4.069802"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PK171_00235"
     CDS             complement(<1..718)
                     /locus_tag="PK171_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT04661.1"
                     /translation="MEELRFFQTPAPHLKSPVSVPKIMFMVVVALIPATVWGIGFFGP
                     KLTLPPLIICILSCLFFEWLDCKIFKKPVTLKDGSALVTGLLLGLSLPPGSPFWIAIA
                     GGGIAIILGKQMFGGLGQNIFNPALVGRAVLLISWPKLMTSWYAKTPFAFPSIEQGLV
                     AMPQGVDVVTTPTALTLTKEYGFQVLSQFEPRLLWRLFIGAVPGSIGETSVLLLLIGY
                     FLLLGLGIVKWDIPLFFLIGL"
     gene            complement(720..2048)
                     /gene="rsxC"
                     /locus_tag="PK171_00240"
     CDS             complement(720..2048)
                     /gene="rsxC"
                     /locus_tag="PK171_00240"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOT04662.1"
                     /translation="MIGKLFHGGIHPPTFKDLTAQKPIKKFPSPDKVYLPLLQHTGTP
                     LQPLVKKGDLVKRGQKIADADAFVTAPLHSPIAGKVLGIEECPHPTRGKFGAIVIENS
                     GSQEDEINGILFQEEIERMDPKSIQNWIREGGFVGLGGAAFPTHVKVSPPSGKKIDTL
                     IINGAECEPFITCDHRVMMERTDFILTGAYLLARACGAQKIIFGIEKNKPDCIEKITG
                     IIHKVPLPTQVFPLPVLYPQGSEKHLILTILGKEVPPGGLPLDVQVVVSNVQTAWAIG
                     RKAQEGIPLIERIITLTGNSLNNPENLDVPIGVRLLDLVDFCGGFQVPPEKTIMGGPM
                     TGIAQVTLEVPVVKGTSGFVFFRENIVKEEYSCIRCGRCVDHCPMYLLPTDLFRYAEY
                     GDFEEAKKQRALDCIECGSCSYVCPAGIPITHYIKIAKAEILLRAKKGGK"
     gene            complement(2088..3434)
                     /locus_tag="PK171_00245"
     CDS             complement(2088..3434)
                     /locus_tag="PK171_00245"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOT04663.1"
                     /translation="MFQKVLIANRGEIALRIVRACREMDIKTVGVFSDIDRNLIHLRD
                     VDQKVALGGNSPAESYLNIDKILKACHLTKADAVHPGYGFLSENVLFVQKLQENGIAF
                     IGPSVDVLQKMKNKLLAKKIIHDSGVPVVPGSLEVVNTLLKAKKIASEIGFPFMLKAC
                     LGGGGRGIRVVENEKNLEEAFNSARREAKMAFGNEEIYIEKILDNARHIEVQILADRF
                     GKTIHLGERECSLQRRRQKILEEAPSPFLNDSLREKICNAAIKAAVALGYSTCGTMEF
                     LTTDDQKFYFMELNARIQVEHPITEMVSGVDILREQIRLASGEPLQLNTNQLNQRGHA
                     IEMRINAEDPFRNFMPTPGVIKTYETPHGPGIRIDSHCYSGYEIPSFYDSLIAKLIVW
                     DTDRIHAIRRSREALHSFIVEGVPTTIPFHLKILQNDDFLRGIFHTRFIEDEIEDI"
     gene            complement(3505..4806)
                     /locus_tag="PK171_00250"
     CDS             complement(3505..4806)
                     /locus_tag="PK171_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOT04664.1"
                     /translation="MFKPAQIILLGYITVILLGSLILMLPAMTAPGKDLTFIDAVFTS
                     TSAICVTGLTVVDTATFFSFWGQIVILVLIQCGGLGYMTLTTLIAIGLGRRIEYRDRL
                     ALKETFSLETPGGVVRFTLNVLKYTFTFEALGAFFLLISFIGRYPLPKAIFASIFHSV
                     SAFCNAGFSIFSNNFEDFVQNPLVNITLCFLIILGGLGFMVLKEIITKRRVITLHSKV
                     VIRVTILLLLAGTLLILLFEWDNPNTIGMLSPGYKVLTSFFQAVTPRTAGFNTVPIDS
                     MRSISLFIIIFLMFIGASPGGTGGGIKTTTFALLLGVVKGVAQEKNRVEYFHRRIPNE
                     TIYRAIAQTILPFLLVAIVSIIIMSYQHDNPTRIIFEVFSAFGTVGLSTGITPTLFSL
                     SKFLLVIMMYYGKVGLLGLAIYPVKKDEKECILYPEEGVQL"
     gene            5022..6020
                     /gene="hflK"
                     /locus_tag="PK171_00255"
     CDS             5022..6020
                     /gene="hflK"
                     /locus_tag="PK171_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOT04665.1"
                     /translation="MENSEFDGKIIHIYDSKIIRLLKKLGYLVIIIFLGIYLLTGIYV
                     VGPDEVGMVKLFGEFVRQVPPGIHYHIPYPFETIIKPKITEVRRVEIGFRTLSTDPIP
                     RYQLIPEESLMLTGDENIVDCQYTVQYRISDPYLFLFRVKDVDAAVKKAAESALREIV
                     GKKQIDEVLTSGKSEIQEETRVLIQSILDRYEAGVQIIAAQLQDVQPPEAVVSAFKDV
                     ASAREDKVRFVNEAEGYRSEVIPNARGQAEQIIKEAEAWQEKVVKEAEGDTARFLQVL
                     QEYSLNKEVTRTRLYLETIQKALPLVQKYLIDSSTASQDILKLLPLTGKDGSAVVY"
     gene            6010..6897
                     /gene="hflC"
                     /locus_tag="PK171_00260"
     CDS             6010..6897
                     /gene="hflC"
                     /locus_tag="PK171_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOT04666.1"
                     /translation="MSINKSFWKKMVLIIIILILISITKPWFTLDETEQGVILQFGKP
                     IAVVQKSGFHLKLPYPLQVIYVFDKRILDYDSPPEEVITEDKKTLVIDNYVRWRIIDP
                     LRFLQTVVSETGAMVRIDDIIYSELRAEIGRIPLFDLISIKRRQVMDTVTVNSNRKAN
                     AFGIEIIDVGIKRADLPKQNEEAVFQRMKTERERQAKQYRSEGQEEAIKLRAETDKEK
                     TLIIAEAEKQSKIIRGQGEAKALRIYAEAFKQDPEFYTFQKTLETYEKTFNNKDVLIL
                     TPDTEFLHYLFKINQEIEK"
     gene            complement(6947..7291)
                     /locus_tag="PK171_00265"
     CDS             complement(6947..7291)
                     /locus_tag="PK171_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT04667.1"
                     /translation="MSSSGNDFFQEYRNGLKRLKDVSPKTLEGFNGFYHQAMADGSLN
                     IKTKELIALGIGLAIHCENCIWLHTRAAIKAGASTEEILEAAQVGVVMGGGPAFTYLP
                     LVQKIIDQLTEQ"
     gene            complement(7297..8223)
                     /gene="ilvE"
                     /locus_tag="PK171_00270"
     CDS             complement(7297..8223)
                     /gene="ilvE"
                     /locus_tag="PK171_00270"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HOT04668.1"
                     /translation="MQELSSIWLNGKIVDWKDATTHVLTHALHYGTSVFEGIRCYKTI
                     QGSAVFRLGDHIQRLFDSARIIKMCIPYSQKELYQATLETIRRNQVEECYIRPLVFRG
                     YGEMGVDPTSCEVISAIAVWRWGAYMGEKGLKHGIRAKISSYTRNSMNASSPRAKTSA
                     NYLNSALAKTEAKELGYDEAILLDMHGFVSEGSGENIFMVKKGVLYTPHPYSILLGIT
                     RDTVIRLALDLEYDVRETHCTRDDLYLADEAFFTGTAAEITPIVEVDGRPIGDGKPGP
                     VTRQLQEIFFRVIRGEEAAYIDWLDYVDKKKE"
     gene            8332..9705
                     /locus_tag="PK171_00275"
     CDS             8332..9705
                     /locus_tag="PK171_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT04669.1"
                     /translation="MKNIANSSKTNTIKEDAGVIATYWDEYYELMPRHNLEKIQLNRL
                     KKVLIRAYHNLPYYRQKIDQAGIDINDVQSLYDLQRLPFTTKQDLRNVYPFGAIAEPL
                     KNIVRIHSSSGTTGTATVVGYTRNDIEIWSQLLARTLCGCGITEQDTIQVAFGYGLFT
                     GGLGVHYGAEKLGATVVPMSSGNTLRQIQILKDFQISALCCTPSYALYLSETAKENQI
                     DWSQTAFRLGIFGAEPWSEEMRIEIEKKLPIRAFDIYGLSEVIGPGVAFECEFHQGLH
                     ISEDCFIPEIVDIQTGKPLPPGETGELVFTTLTKEGTPVIRYRTGDISSLKYGECQCG
                     RTMARMERVTSRTDDMLIIRGVNVYPSQIESVILSFDGIEPYYQILISRENFLDKVEV
                     EVEVSSEIFSDEIKVLENLRNKIEEKIKAELNLSCSIKLIKPKSITRSEGKAKRVIDL
                     RKKEGFS"
     gene            9702..10118
                     /locus_tag="PK171_00280"
     CDS             9702..10118
                     /locus_tag="PK171_00280"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT04670.1"
                     /translation="MKQISVFLENKPGSLHAVLAKLKEWNVNLRALSLADTADFGVLR
                     FIVEKPEQLVEQLRKENFTATLTEILVVGVDDRPGGLSQIVEVLTLNHISIEYLYAFV
                     SPKGQKAYVVMRVEDVPRASHILREAKASLLDEITI"
     gene            complement(10199..>11387)
                     /locus_tag="PK171_00285"
     CDS             complement(10199..>11387)
                     /locus_tag="PK171_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04671.1"
                     /translation="WSMSPKTLLLLSNGYGEDSFASILLEKLVQFTKVYKIPSTFLVI
                     PLIGKGAQFYQCQSQYPDQVSIITPPFSLPNGGVYLGSAWQKSKRFIEDCFRGVIPNS
                     GFILRELKKIKNKIDAVIGIGDFIPPLLNQLFLQKELYMVACAHTWLLKRSNQPLERL
                     GRLTRHLFRYGCRQVYTRDRLTEQWFLQLGITAKYLGFLGPDIKKKEENRRKIVFLPG
                     SRKDWKNNLQFFFVAFKEVNHQYLKDYEIHIVFSPGVDTQEIQFLLASLLKNHHFFIS
                     WSQGDYFQHLSQSALVVGFAGTAIEQAAFLGIPSIEPWQENAIQVNRDFIENRQKLLL
                     REALIPGGDTPSQLAQVLKNTLLNLQDYQKAAENFSQKVWEGKSNGGMTIAENIMMLL
                     INQ"
BASE COUNT         3631 a         2378 c         2065 g         3313 t
ORIGIN      
        1 agagaccgat aagaaaaaag agtggaatat cccattttac aattcctaat cccaataaca
       61 aaaaataacc aatgagaaga agtaatactg aagtttcacc gatacttcca ggaacggcac
      121 cgataaaaag ccgccagagt aagcggggct caaattgaga aagaacttga aacccatatt
      181 ccttggttaa ggttaatgct gttggagtgg ttaccacatc aactccttga ggcatagcga
      241 ctaaaccttg ttcaatgctg ggaaaagcaa agggagtttt cgcataccag gaagtcatta
      301 gtttcggcca agaaataagc agaactgccc gaccaaccaa ggccggattg aaaatatttt
      361 gacctaatcc cccaaacatt tgttttccta atattatagc tattcctcct ccagcaatag
      421 caatccaaaa aggcgaaccc gggggaagag ataacccaag taaaagacca gtcaccaagg
      481 cactcccatc ctttaaggtc actggtttct taaaaatttt acaatcaagc cactcaaaaa
      541 aaagacagct taaaatacaa attataagag gtggaagagt taacttagga ccaaaaaaac
      601 cgatacccca aactgttgcg ggtatcaaag ccacaacgac catgaacata attttcggaa
      661 cagaaactgg acttttcaaa tgtggtgccg gggtttggaa aaagcgcagt tcttccattt
      721 tatttccctc ctttctttgc ccgaagaaga atctcagcct ttgcaatttt aatgtaatgg
      781 gttatcggta tcccggcggg acatacgtaa gaacaactcc cacactcaat gcaatcaaga
      841 gctctttgtt tttttgcttc ctcaaaatca ccatactcag catatcgaaa taaatcggtt
      901 ggtaagagat acattggaca gtggtcaacg caccgtccac agcgtataca ggaatactct
      961 tcttttacaa tattttccct aaaaaaaaca aagcccgagg ttccctttac taccggcact
     1021 tccaaggtaa cctgagcaat tccggtcatc ggtcctccca taatagtctt ttcaggaggt
     1081 acttgaaaac ctccacaaaa atcgaccaaa tccaataaac gcactccaat tggaacatca
     1141 agattttccg gattatttaa actatttcca gttaaagtaa tgattctttc aattaatgga
     1201 attccctctt gagcttttcg cccaattgcc caagcagttt gtacgttgct cactaccacc
     1261 tgaacatcaa gaggtaaacc tcccggtggt acttcttttc ctaatatggt taagattaaa
     1321 tgtttttctg aaccctgagg atacaaaacc ggaaggggga aaacctgcgt gggaagcggg
     1381 accttatgaa taatcccggt tattttttct atacagtctg gtttattttt ttctatacca
     1441 aatataattt tttgagctcc acaagcccga gctaaaaggt aagcaccggt caaaatgaaa
     1501 tcagtccgtt ccatcatgac ccggtgatca caagtaataa aaggttcgca ctctgctccg
     1561 ttgataatca gggtatcaat tttcttccca gaaggagggc taacctttac gtgtgttgga
     1621 aaagccgcgc ctcctaaacc aacaaaacct ccttctcgaa tccaattttg aatactttta
     1681 ggatccattc tttctatttc ttcttgaaac aaaatcccat ttatttcatc ttcttgcgaa
     1741 ccactatttt ctataactat tgctccaaac ttacctcggg ttgggtgtgg acattcttca
     1801 attcctaaaa ccttcccggc aataggggaa tgaagaggcg cggtaacaaa ggcatcggca
     1861 tcggcaattt tctgacctct tttgaccaga tcgccttttt tcactaatgg ttgcagtgga
     1921 gttccagtgt gttgaagaag gggaagatac actttatctg gagagggaaa ttttttaatt
     1981 ggcttttgag ccgtcaaatc cttaaatgtc ggaggatgaa tgcccccgtg aaacaatttt
     2041 cctatcatct tttcttcctc catctaaacc atagaaaaat ttatcgatca aatatcttcg
     2101 atttcatctt caataaaccg tgtatgaaaa atacccctta agaaatcatc attttgtaaa
     2161 attttcaaat gaaaaggaat cgtagtcggc accccttcaa caataaagga atgaagtgct
     2221 tctcgtgaac gacggattgc atgaattcga tcagtatccc aaacaatgag cttagcaatt
     2281 aaagaatcat aaaaagatgg aatctcatat cctgaataac aatgcgagtc aatacgaatc
     2341 ccaggtccgt gaggtgtttc ataagttttt atcacacctg gtgttggcat aaaattgcga
     2401 aaaggatcct cggcatttat tctcatctca atagcatgac cccgttgatt caattgattg
     2461 gtgtttaatt ggagtggttc gcctgaggct aaccgaattt gctcccgaag gatatccaca
     2521 cctgatacca tctcggtaat tggatgctca acctggatac gagcatttaa ctccataaaa
     2581 taaaacttct ggtcatcggt ggttaaaaat tccatcgtac cacaagtaga atatcctagg
     2641 gcaactgccg ccttaattgc tgcattacat attttttccc ttaaactatc atttaaaaaa
     2701 ggagatggag cttcttctaa tattttttgc ctacgccgtt gtaaggaaca ctctctttcc
     2761 cccaaatgaa tagtttttcc aaatcgatca gctaaaattt ggacttcgat atgtcgagca
     2821 ttatccagta ttttttcaat ataaatttct tcattcccaa aggccatttt cgcttcccga
     2881 cgggcactat tgaatgcttc ttctaaattc ttttcattct caaccactcg gatacctctc
     2941 ccacctcctc ccaaacaggc tttcaacata aatggaaaac ctatttcact ggcaattttt
     3001 ttggctttta gtaaagtatt aaccacctcc agggaaccag ggactaccgg tacacctgag
     3061 tcatgaatta tttttttggc taagagttta ttcttcattt tttgtaaaac atctacacta
     3121 ggtccgataa aagcgatccc attttcctgt aatttttgaa caaaaagaac attttcggat
     3181 aggaagccat agcctggatg aactgcatcg gctttggtga ggtggcaagc cttcaaaatt
     3241 ttatcaatat taagatagga ttcagctggg gagtttccac ctagcgcgac tttttgatca
     3301 acatctcgga gatggataag atttcgatca atgtcagaaa agaccccaac tgttttaata
     3361 tccatttcac gacaagctcg gacaatcctc agcgcaattt ccccgcggtt cgcaatcaat
     3421 actttctgaa acaaaaaaat cacctcgtat ccaactgtat catatagttt atttagagat
     3481 gataattacc ttataaaaat cgatttaaag ctgtactccc tcttcgggat aaagtatgca
     3541 ttccttttca tcttttttta ccggatatat ggctaaaccc aataatccca cttttccata
     3601 atacatcata atcacaagaa gaaacttgct caaactaaat aatgttggtg ttatcccggt
     3661 cgataatcca accgtcccaa aagccgaaaa aacttcaaaa ataattcgag taggattatc
     3721 atgctgataa ctcattataa taatggatac tatcgcaact aataaaaaag gaagtattgt
     3781 ttgggcaatt gcccggtaaa tggtttcgtt tgggatccgg cgatggaagt attcaactcg
     3841 gtttttttct tgagccacac cttttaccac acctaatagc aaagcaaaag tagtggtctt
     3901 aatcccaccc ccggttcccc ctggagatgc tccaataaac ataagaaaga taataataaa
     3961 taaagaaata gaacgcatcg aatcgattgg aaccgtatta aatcccgccg ttcgtggagt
     4021 taccgcctga aaaaaacttg ttagcacttt ataaccggga ctcagcattc caatagtatt
     4081 cggattatcc cactcaaaaa gcagaatgag taaagttcca gccaacaaca aaagaatagt
     4141 gactcggatc actaccttac tatgaagtgt tatgactcga cgtttggtaa tgatttcttt
     4201 gagaaccata aaacccaatc cgcccaagat gatcaaaaaa cataaagtta tattaaccaa
     4261 agggttttga acaaaatctt caaaattatt tgaaaatatt gaaaatccag cattacaaaa
     4321 agccgaaaca ctatgaaata ttgacgcaaa tattgccttg ggtaaaggat atcgaccgat
     4381 aaaactgatt aataagaaaa aggctcccaa ggcttcaaag gtaaaggtat attttaatac
     4441 attgagcgta aaccgtacga ctccaccagg agtttccagg ctaaaagttt cctttaaagc
     4501 aagacggtca cgatattcaa tccgtcgacc gagaccaatg gcaattagag tcgtgagggt
     4561 catgtatccc aaaccaccac attgaataag aaccaaaata acaatttgac cccaaaagga
     4621 aaaaaatgtt gcggtatcga ccacggtcaa accagtcaca cagatggcag atgttgaggt
     4681 aaaaacagca tcaataaaag tcaggtcttt ccccggggca gtcatagcag gcagcataag
     4741 gatcaatgaa ccaagaagaa taacggttat atatcccaat aaaataattt gagcaggttt
     4801 aaacattata tgccgctcat gattgatcga accaaagact gatgtacttt ttctagtatc
     4861 ccgaataacc aacataattc aagtatgatt tatctttttt taaatattct ttatcttcta
     4921 agaaaccgag tttgaaccaa agatgtattt atttcaatac aatcttgact attttatcac
     4981 ataacttgcg caggattaaa tcgtagtgta gtattataaa catggaaaat agtgagtttg
     5041 atggaaaaat tattcacatt tatgattcca aaataatccg tttattaaaa aagctcggtt
     5101 atttagtgat tatcattttc ctcggaatat acctgcttac tgggatttat gtagttggcc
     5161 ctgatgaagt tgggatggtt aaactttttg gtgaattcgt ccggcaggtt cctccaggta
     5221 ttcattatca tattccctat ccttttgaaa ctatcattaa accgaaaatt actgaggtcc
     5281 gccgtgtgga aattggattt agaaccctca gtaccgatcc tattccccga tatcaactca
     5341 ttcctgaaga atcattgatg ctaaccggag atgaaaatat tgtcgactgc caatatacag
     5401 tacaataccg aattagcgat ccttacctat ttctcttccg agtgaaagat gtcgatgctg
     5461 ccgtaaaaaa agcggccgaa tcagctcttc gggaaattgt cggaaaaaaa caaattgatg
     5521 aggtcctcac ttcaggaaaa agtgaaatcc aagaagaaac cagagttctt attcagtcca
     5581 ttttagatcg atatgaagca ggtgttcaga ttattgccgc tcaacttcag gacgttcaac
     5641 ctccagaagc agtggtttca gcttttaaag atgttgccag cgctcgggaa gacaaagttc
     5701 gttttgttaa tgaggccgaa ggataccgta gtgaagttat tcccaacgct cgtggacaag
     5761 ccgagcaaat tatcaaagaa gccgaagctt ggcaagaaaa agttgtgaaa gaagccgaag
     5821 gagacacggc ccgtttcctc caagttctcc aagaatatag cttgaataaa gaagtcactc
     5881 gaacccgcct ttatctcgaa accatccaga aggcgcttcc tctggttcaa aaatatctga
     5941 tcgatagttc cacggcttcc caagatattc taaagttact tcccttaact ggaaaggatg
     6001 gttcagccgt tgtctattaa taagtcgttc tggaaaaaaa tggttttgat tatcatcatt
     6061 ctgattctta tttcaattac caaaccctgg tttactcttg acgaaaccga acaaggagtc
     6121 attctccagt ttggtaagcc gattgcagtg gtgcaaaaaa gcggatttca tctgaaacta
     6181 ccctatcctt tacaggttat ttacgttttt gataaacgta ttcttgatta tgattcacct
     6241 ccggaagaag tcattactga agacaaaaaa actctggtga tagataatta tgttcgatgg
     6301 cgcattatcg atcctctccg ctttctacaa actgtggtga gtgaaaccgg tgcaatggtt
     6361 cgaatagatg atattatcta ttcggagtta agagctgaaa ttggccgcat tcctctcttc
     6421 gatttaattt caataaaacg tcgtcaggtt atggatacgg ttactgtcaa ttctaaccga
     6481 aaagcgaatg cttttgggat agaaattatc gatgtaggaa ttaaaagagc cgaccttccc
     6541 aaacaaaatg aagaagcagt tttccaaaga atgaaaactg aaagagaaag acaggcgaaa
     6601 caataccgtt ccgaaggcca ggaagaggct atcaaattac gggcggaaac cgataaggag
     6661 aaaactctga taattgccga agcggaaaag caatcgaaaa ttattcgtgg ccaaggggaa
     6721 gctaaagctt tacgaattta tgccgaagcc ttcaaacagg atccagaatt ctataccttt
     6781 caaaaaacct tagaaaccta tgaaaaaact ttcaacaata aagacgtttt gatcctgaca
     6841 ccggatactg aatttctcca ctacttattt aaaatcaatc aagaaattga aaaatgaact
     6901 ttaaaaaaac cggtgggagt aattcccacc ggttaaccaa aaggatttac tgctcggtca
     6961 gttggtcaat gattttctga actaaaggta agtaagtaaa agctgggcct ccacccatga
     7021 ccactccaac ttgagctgcc tctaagattt cttcagtact ggcaccagcc tttatcgctg
     7081 ctcgagtgtg taaccagata caattttcac agtgaatagc caatccaatt cctaaggcaa
     7141 ttagctcttt ggttttaata ttgagacttc catcagccat agcttgatgg taaaaaccat
     7201 taaaaccttc caaggttttt ggtgaaacat cttttaatct tttcaaacca tttcgatatt
     7261 cttgaaaaaa atcatttcct gatgaactca tgcaacctac tccttctttt tgtcaacata
     7321 atcaagccaa tcgatataag ctgcttcttc tcctcgaatc actctaaaaa atatctcttg
     7381 taattgccgg gtcactggtc ctggctttcc atcaccaatt ggtcttccgt caacctcaac
     7441 aatgggagta atttctgctg ccgtcccagt aaaaaatgct tcatcagcca gataaaggtc
     7501 atctcgagta cagtgagttt ctctgacatc atattctaaa tcgagtgcca aacgaataac
     7561 tgtatctcgg gttatcccta gtaatattga ataaggatgg ggcgtatata acactccttt
     7621 tttaaccatg aaaatattct caccgcttcc ttctgaaaca aatccgtgca tatccaaaag
     7681 gatagcttcg tcataaccca attctttagc ttcggttttt gctaatgctg aattgagata
     7741 gttggcgctg gtttttgctc ggggagaact ggcattcatt gaattacgag tataggatga
     7801 aattttagct cgaattccat gttttaaacc tttttctccc atataggcac cccatcgcca
     7861 aaccgctatt gctgaaatga cttcacaact ggttggatca acccccatct caccataacc
     7921 acggaagacc aaaggcctta tatagcattc ttctacttga ttccttcgga tagtttcaag
     7981 cgttgcttgg tacaattctt tttgactata agggatgcac attttaataa ttcttgccga
     8041 atcaaacaac ctttgaatat gatctccaag ccgaaacacc gctgatcctt gaatggtttt
     8101 atagcaacgg attccttcaa acaccgacgt tccgtaatga agggcatggg ttaatacatg
     8161 ggttgtcgcg tccttccaat caacaatttt tccattcaac caaatcgaag ataattcctg
     8221 cattcaaacc accccgtaat tctattaatt atattttatt cagagtataa cactcttttc
     8281 agtaaaatga gctattctca tccgcatcct ataaaaaaat aacccaattt cgtgaaaaac
     8341 attgcaaact cgtcaaaaac gaatacaata aaagaagatg cgggggtgat agcgacgtat
     8401 tgggatgaat attatgaatt aatgccacgt cataatcttg aaaaaattca attaaatcgt
     8461 ttgaaaaaag ttttaattcg tgcttatcat aatcttccct attatcgaca aaaaattgat
     8521 caagccggta ttgatattaa tgatgttcaa tcactttatg accttcaacg ccttcctttc
     8581 acgactaaac aggatttaag gaatgtttac ccgtttggag ctattgctga acctttaaaa
     8641 aatattgttc ggatccattc ttcatcaggt accaccggaa cagcaacggt ggttgggtac
     8701 acccgtaatg atattgaaat ctggtctcaa ctattagctc gtaccctttg tggatgtggc
     8761 ataaccgaac aggacactat tcaagtggct ttcggatatg gtttatttac cggagggtta
     8821 ggagtccatt acggagcaga aaaattaggt gccactgtcg tacccatgtc cagtgggaac
     8881 actcttcgtc aaattcaaat attaaaagat ttccagattt cggctttgtg ttgtactcct
     8941 tcttatgctc tttatctttc agaaacagcc aaagaaaacc aaattgattg gtcgcaaact
     9001 gcctttcgct tgggaatatt tggcgccgaa ccttggtctg aagagatgag aatagaaatt
     9061 gaaaaaaaac ttcccattcg agcctttgac atttatggat taagcgaggt tattggcccg
     9121 ggagttgcat tcgaatgtga atttcatcaa ggtcttcata tttctgaaga ttgttttata
     9181 cctgaaatag ttgatattca aactgggaag cccttacccc ccggcgaaac tggtgaactg
     9241 gttttcacca ctttaaccaa agaaggaaca ccggtaatcc gatatcgaac tggtgatatt
     9301 tcttctctta agtatggtga atgtcaatgc ggccgaacca tggcaagaat ggaacgggtc
     9361 acatcacgaa ccgatgacat gctcattatc cgtggagtaa atgtttaccc ctcacaaatc
     9421 gaaagcgtca tcctgagttt tgatggaatt gaaccctatt atcaaattct gattagtagg
     9481 gaaaatttct tagacaaagt cgaagttgag gttgaggtat cttcagaaat attttctgat
     9541 gaaattaaag ttcttgaaaa cctcagaaat aaaattgaag agaaaattaa agccgagctg
     9601 aatctttcct gttcgataaa acttattaag cccaaaagca ttactcgttc tgagggtaaa
     9661 gcaaagcggg tcatcgacct gcggaaaaaa gagggattct catgaaacaa atatcggttt
     9721 ttttagaaaa taagccagga agtcttcacg cggttttagc caagctgaaa gaatggaatg
     9781 ttaatctccg cgccctatcg ctggctgata ctgctgactt tggagtactc cggtttatcg
     9841 ttgaaaaacc agagcaactg gttgagcagc ttcgcaaaga aaattttact gccactctca
     9901 ccgaaatatt ggtagttggt gtagatgatc gaccgggagg actttctcaa atcgttgaag
     9961 tactcaccct aaatcatatc tccatcgaat atctctatgc ttttgtttct cccaagggcc
    10021 aaaaagccta cgtggttatg agagttgaag atgtcccacg tgcctctcat attttgcgag
    10081 aagcaaaggc atctttactt gatgaaatta ccatataaaa aagcttttta aaaagtttct
    10141 taccagaaac caggggatag tattaaaact atcaaccagt gaccgattca ttactctgtc
    10201 actggttgat taataacatc ataatgtttt cagcaatagt cattccacca ttggattttc
    10261 cttcccaaac tttttggcta aaattttcgg ctgctttttg atagtcttga agattcaaaa
    10321 gagtattttt aagaacctgg gctaactgag atggtgtatc tcctcctgga attagtgctt
    10381 cccgaaggag aagtttttgt cggttttcaa taaaatcccg attgacttga atggcatttt
    10441 cttgccaagg ttcaatagag ggaattccca aaaaagctgc ctgttcaata gctgtaccag
    10501 caaaaccaac gactaaagct gattgagaaa gatgctgaaa ataatcacct tgactccaag
    10561 atataaaaaa atgatgattt ttcaaaagtg atgccaataa aaattgtatt tcttgagtat
    10621 caacacccgg tgaaaaaact atatggatct catagtcttt taggtattga tgattcacct
    10681 ctttaaaagc tacgaaaaaa aactgtagat tattcttcca atcctttcga cttcccggca
    10741 aaaaaacgat ttttcttcta ttctcttcct tttttttaat atctggtccc aaaaatccta
    10801 aatattttgc agtaatgccc agctgaagaa accattgctc cgttagccgg tctcgagtat
    10861 aaacctgtcg gcaaccatat cggaagagat gccgggtcaa tcgacccagt cgctccaaag
    10921 gttgattact tctttttaac aaccaggtat gggcacaagc caccatatat aattcttttt
    10981 gtaaaaataa ctggttcaat aaaggtggaa taaaatcacc gataccaatg accgcgtcaa
    11041 ttttgttttt aatttttttc aactcgcgca aaataaatcc gctattgggt ataactccgc
    11101 gaaagcaatc ttcaatgaat cttttcgatt tttgccaagc agaacccaag tatactcctc
    11161 catttggcaa tgaaaaaggg ggagtaataa ttgacacttg atcgggatat tgcgactggc
    11221 attgataaaa ttgtgcccct ttcccgataa gaggaataac caaaaaagtg cttggaattt
    11281 tatatacttt ggtaaattga accagttttt ctaataaaat tgaggcaaat gaatcttccc
    11341 cgtatccgtt gctgagaagc aataatgttt ttggtgacat tgaccat
//
LOCUS       DAOSOL010000005        11045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_5898_length_11045_cov_4.483258, whole genome shotgun sequence.
ACCESSION   DAOSOL010000005 DAOSOL010000000
VERSION     DAOSOL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11045
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5898_length_11045_cov_4.483258"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="PK171_00290"
     CDS             <1..1850
                     /locus_tag="PK171_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOT04672.1"
                     /translation="GRKRIVVEKIPDQENFFKNELLKITKPARYIGEEWNILVKNPSQ
                     TTCSVVLGFPDIYEIGMSHLGLKILYQILNDLPGVRAERAFVPWPDLGKLMKEKSIPL
                     FSLENKIPIRSFDIVGLSLQTEMNFTNILYFLDLSQIPFRSSEREDDFPIIIGGGAST
                     CNPEPISDFFDAFLIGEGEEAFVEIIQIVSAMKGQRKGDILSELAKVVGVYIPSFYQV
                     GYSKNGIVSKIQTMNKRAFPQIQRRWLKDLDNALYPEAIPVPYISIVHDRIPLEISRG
                     CTRGCRFCQAGMIYRPHRERSPENIVHLAEKLVTSTGYEEISLLSLSSTDHSRIEEII
                     SNLSRRFEAKNVNISLPSIRMDTFSVQIAQNLKRVRKSGLTFAPEAGTDRLRRIINKG
                     LTDQEIFSTLEKVFEGGWDDVKLYFMFGLPDEKESDLIGISQLVNEGLQIGKKIRGKR
                     VSIHLNLSAFVPKPHTPFQWMGQNSLEEFEEKIQRIKAGFSRDRRLIRMNWSDFKEST
                     LEAVLSRGDRRLSRVIEKAYHLGQIMDGWREFFSFERWKLAFEEVGLDYHFYSERWRD
                     PKEILPWDHISSGVKKEYLLKELEKSRRQEVTPRCIHFQECMSCGVCSS"
     gene            1847..2446
                     /locus_tag="PK171_00295"
     CDS             1847..2446
                     /locus_tag="PK171_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOT04673.1"
                     /translation="MNFTDRYRYRCRHLKLVPFHLLSQLDLTRFWDRVLRRAGLPIVF
                     SQGFNPRPLLSFGPATVTGVISWTEYLDMTFYEFIEPEQLKNILNSQVLEEMRVVEVN
                     SIPLDFPSIMKSLISVQYAFIFKDEGNIGNHQVLFSIEDIEELERKKMEKQECMISFL
                     FKKKNILLNPYKSLEFMPKESGFNRENLVEIIKQNYYWF"
     gene            2465..3946
                     /locus_tag="PK171_00300"
     CDS             2465..3946
                     /locus_tag="PK171_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT04674.1"
                     /translation="MKKDIVIDMSEAEVRAALLENGQVVEFFFERAAEKRLAGNIFKG
                     IVENVLPGIQSAFVNTGFDKNAFLFIGDILLDEKGKIKSIDQLLQTGKEIVVQVAKEP
                     MGTKGARVTTKISLPGRYVVLMPGMNNMGISHRISSSEERDKLKKLIEKIRPANMGVI
                     VRTVAEGKSNEEIKEDIESLLRLWNRIMKKAELISAPAVLYEEASLIYTLIRDVVDDN
                     LGEIWINSYFGFQKLKDFIESMIPRFVDRIHFFDSKENIFDHFNLNRELEKALSRKVW
                     LKSGGYLVFDRTEAMTVIDVNTGKYIGKKDLQETILKTNLEAAEEIARQVRLRDIGGI
                     ILIDFIDMGKKEHQKMVMNVFEQFLSHDRTRSSLVQMSELGLVEMTRKRVRKNLDAIL
                     CQPCNCCQGEGRVLSMETIAINFLRRVEEICRHSQSTTIGFTVGQELGDYLKNNGKIF
                     LNNLKKVYSKELVWKIDPNLSLRRYSIVGVGEDGIRESIDKIE"
     gene            4092..4403
                     /gene="rplU"
                     /locus_tag="PK171_00305"
     CDS             4092..4403
                     /gene="rplU"
                     /locus_tag="PK171_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT04675.1"
                     /translation="MFALIEASGKQYPVQEGSVIQLDSYQGNLKDEVVFERVLFIRNQ
                     DQVVVGRPYVEGAKVKGIVLRNGKSKKVVVFKYKPKVKYRRLTGHRQPMTLIKIQSIE
                     S"
     gene            4427..4696
                     /gene="rpmA"
                     /locus_tag="PK171_00310"
     CDS             4427..4696
                     /gene="rpmA"
                     /locus_tag="PK171_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT04676.1"
                     /translation="MAHKKAGGSGKNGRDSNAQRLGVKKFSGQYVLAGNILIRQRGTK
                     IKPGINVGTAKDYTLFALQSGYVVYQEKAGRKYVSILPEMPSLDS"
     gene            4805..6085
                     /gene="obgE"
                     /locus_tag="PK171_00315"
     CDS             4805..6085
                     /gene="obgE"
                     /locus_tag="PK171_00315"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT04677.1"
                     /translation="MFVDQVILKVRAGRGGDGAISFLREKYVPRGGPAGGNGGKGGNV
                     IAIALSQKKTLYDVAIQKVYEASNGEPGKGKNQFGKDGADVVLVVPVGTQIIDQKDNQ
                     IIADLTEDKQEVIIARGGRGGRGNSSFATSTNQAPRIAEKGELGEAREIRLELKLIAD
                     VGISGFPNAGKSTLLSVVSAAKPRIADYPFTTLHPNLGVVNHHDQQFIMVDVPGIIEG
                     ASRGAGLGLEFLRHIERTRLILHCVDLSQFYSENNPYDDFLTLRREFEAYSSSLAAKP
                     FFVVATKLDLIKAAQNFNEFKSTLEQQGIPVFGVSSLTKEGMNQLLDAIIEFLKNAPE
                     IFTKEILSQSKNTPLSPIRSRHYRFSTPFLDRLIAEIDWNSDNALSIFNQKLVHFDFL
                     KYFSAIQPGSIVEIENLNFIWTGKRLEAHKTTSK"
     gene            6102..7247
                     /gene="proB"
                     /locus_tag="PK171_00320"
     CDS             6102..7247
                     /gene="proB"
                     /locus_tag="PK171_00320"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOT04678.1"
                     /translation="MKRIDFIENSRVVVIKVGSSLLVKNFVLEIPRLEELARNIHQLR
                     QSGKKVILVTSGAMACGMSRLGLKRKRNDIPFKQAMAAVGQGILMQNYCQVFSRIGIP
                     VAQILLAPEDVHNRHKYLNARNTFQTILDFNVLPIVNENDTVAIAELKFGDNDRLSAL
                     VASLINAQLLIILSDIEGLFTSDPKKNSEAILIKEVERIDDKIERMAGREGSNISTGG
                     MKSKIMASRMATFSGIGVILALGKDFSIIQRIFAGDEVGTFFYPQTKVLKNRKRWIAF
                     GMMTRGRVIIDEGAYFALMRNKSLLPAGIKGVEGVFDSGDCIEVVNEEGQSVAKGLVN
                     FSSTELKKIRGLHTTELQEVFFDQEINTEVIHVDNLVLFNREEKDNG"
     gene            7240..8493
                     /locus_tag="PK171_00325"
     CDS             7240..8493
                     /locus_tag="PK171_00325"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOT04679.1"
                     /translation="MAEDMKVQVQKAKKAAQLLATVSTQKKNDFLGDLAQRLEGEQSR
                     IEQENQKDVKIAVENNMKSGFIDRLILNKKRIQGMADGLRQVAALADPVGEVMSGWKR
                     PNGLMVTKVRVPLGVLAIIYEARPNVTIDSIGLGIKSGNSLVLRGSSSTLNSNRLLIL
                     LARESLRQYSLPEDSVQLIESTSHDDIAQLLALREYIDVAIPRGGTGLIHNVVTHSQV
                     PVIETGVGNCHVYVDKEADLGMAERIVMNAKIQRPSVCNAVETLLIHQDIAKIFLPQV
                     SMSLKKAGVEIRGCEQARQILPDLIPATEDDWKTEYLDLILAVKVVKTLNEAIEHINT
                     YGTRHSESIITENYSNAKNFMEKVDSAAVYVNASTRFTDGEQFGLGAEIGISTQKIHA
                     RGPMGLQELTTIKYVVYGTGQIRNA"
     gene            8546..9133
                     /gene="nadD"
                     /locus_tag="PK171_00330"
     CDS             8546..9133
                     /gene="nadD"
                     /locus_tag="PK171_00330"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT04680.1"
                     /translation="MKKGIMGGTFDPIHLGHLVTAEEVRDYFHLDEVVFVPSARPPHK
                     VGQVITSPEHRYLMVVLATITNPYFRVSRVELERVGPSYAVDTLRYFREFWGDESEIY
                     FITGADAFAQISSWNNPEELFRLCTFVAATRPGYKMVSMEEKYRQKVKMIEVSALAIA
                     SSEIRRRVKKGESIKYLVPEAVENYIKKNGLYVNE"
     gene            9126..9719
                     /gene="yqeK"
                     /locus_tag="PK171_00335"
     CDS             9126..9719
                     /gene="yqeK"
                     /locus_tag="PK171_00335"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOT04681.1"
                     /translation="MNEYITKQLKNMISPSRFNHSIRVAEEARTLAVDFGEDPEKSYL
                     AGLLHDSARALACKEMVQYIPPFFLPIGYSIPAIFHAFAGPRFIAEQFGITDYSILRA
                     VCWHATACEDMSNFDKILFVADLSESGRRFQESDTIREALQRGLQVAFTKTLEMKVNY
                     LLSTKKIIYPASLEAWNKIIGISIEEANEKKQKKEKL"
     gene            9688..10593
                     /locus_tag="PK171_00340"
     CDS             9688..10593
                     /locus_tag="PK171_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT04682.1"
                     /translation="MKRSRRKKSYKYLYLAIGFIIGGILFFGLNNHNIRNKLVQTVMG
                     YAPPESTTILVVGQDSIKPLRSDTIILLCLNTKSEEILLFSIPRDTRIEVPGNGYDKV
                     NHSYAKGGIQLLKETIEKNLDIHIPYTVEINYQGFEKVIDLLGGVEIDVESDMKYTDR
                     AQDLVINISAGKQRLKGDKALQYVRYRNDKLGDIGRIKRQQNFLQALFHEIDNPLNLP
                     KMPQIIQELRQALITNFSNEELINLGLWFKDLNERVMITDMMPGEAAYIEGVSYWEPD
                     LDTSKKLVTEFFLKEKRVEEQPNES"
     gene            10583..10957
                     /gene="rsfS"
                     /locus_tag="PK171_00345"
     CDS             10583..10957
                     /gene="rsfS"
                     /locus_tag="PK171_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT04683.1"
                     /translation="MNPKEKCLIVLDVADEKKMQDVVILDLRDVFPVADYWVIGSAAT
                     FIQTKAIAEEIKKTLDSYSVQPFHIEGFDVGEWILMDYGDVIVHIFREEERHYYLLEK
                     LWRNAHLVYSKELQINKINEID"
BASE COUNT         3498 a         1842 c         2405 g         3300 t
ORIGIN      
        1 tcgggaggaa acgaatagtg gtagaaaaaa taccagatca agagaatttt tttaaaaacg
       61 aattgctcaa gattaccaaa ccagcacgtt atataggaga agaatggaat atacttgtaa
      121 aaaatccttc ccaaacgacc tgctcagttg tgcttgggtt tcccgatatc tatgaaatag
      181 gaatgtctca cttagggcta aaaattcttt atcagatatt aaacgacctt cccggggttc
      241 gtgctgaacg agcttttgtc ccttggccag atttaggaaa actcatgaaa gaaaagagca
      301 tcccgctttt tagcttagag aacaaaatcc ccatccgatc ttttgacata gtggggctta
      361 gcctgcaaac cgagatgaat tttaccaata tcctttattt tctcgattta tctcaaatcc
      421 cttttcgaag ttcggaaagg gaagatgact ttcctattat cattggtggt ggagcatcaa
      481 cctgtaatcc agagcccatc agtgattttt ttgatgcttt tttgattggt gaaggggaag
      541 aggcttttgt agaaatcatc caaattgttt cggctatgaa aggtcaaaga aaaggtgata
      601 ttttatccga attagcaaaa gttgtgggag tttatatacc aagtttttat caggttggtt
      661 attcaaaaaa tggcattgtt tcaaaaattc aaaccatgaa taaaagggca tttcctcaga
      721 ttcagagaag atggcttaaa gatttagata atgctctcta tccagaggca ataccagttc
      781 catatataag catcgttcat gatcgaattc ccctggaaat ttcacggggt tgcacccgag
      841 gttgtcgttt ttgtcaagcg ggcatgatct atcgacctca ccgagaacga tctccggaaa
      901 acatagttca tctcgcagaa aaattggtga cttcaaccgg ttacgaagaa atatctcttc
      961 tttctttaag tagcacggat cattctcgga tcgaagaaat catctccaat ctctctcgac
     1021 gatttgaagc aaaaaatgtt aatatttcac ttccttccat acggatggat actttctcgg
     1081 tccaaattgc tcagaattta aaacgagttc ggaaaagtgg tctcactttt gctcctgaag
     1141 caggtaccga tcgtcttcgc cgaataatca ataaaggatt aaccgatcag gaaatatttt
     1201 caactttaga aaaagttttt gaaggtggtt gggatgatgt taaactttac tttatgtttg
     1261 gacttcctga tgaaaaagaa tcggacctga tcggaatttc ccagttggtg aacgaaggtc
     1321 ttcaaatcgg aaaaaaaatt cgcggtaaaa gagtgagcat ccatttaaac ctttccgctt
     1381 ttgtgccaaa accacatact ccttttcaat ggatgggtca gaatagtctg gaagaatttg
     1441 aagaaaaaat ccagagaata aaggctggtt tttccaggga tcgtcgcttg atccgtatga
     1501 attggtcgga ctttaaggaa agcaccctcg aagcagtatt atctcgcggt gatcggaggc
     1561 tatcaagagt gatcgaaaaa gcctaccatt taggtcaaat tatggatggt tggcgggaat
     1621 tcttttcttt tgaaagatgg aaactagcct ttgaagaagt aggtttggat tatcactttt
     1681 attcagaaag atggcgagac ccaaaagaaa ttcttccctg ggatcatatc tcctcaggag
     1741 tgaagaagga atatttatta aaagagttgg aaaaatctcg ccgccaagag gttacaccca
     1801 gatgtattca ttttcaggaa tgtatgagtt gtggggtttg ttcatcatga attttaccga
     1861 tcgttatcgt tatcgttgtc gacatctcaa attagtgcct tttcatcttc tttcccagtt
     1921 agatcttact cgattttggg atcgagtctt acgaagggcg gggcttccga tagttttttc
     1981 tcaaggtttt aatcctcgtc ctttgttatc atttgggccg gccactgtta ctggtgttat
     2041 aagctggacc gaatatcttg atatgacctt ttatgaattt atagaacctg aacaactcaa
     2101 aaacattctt aattcacaag tactggaaga aatgagagtt gttgaagtca actcaatccc
     2161 tctcgacttt cccagcatca tgaaatcgct gattagcgtt caatatgcct ttatttttaa
     2221 agatgagggt aatattggca atcaccaggt attattctcc atagaagata ttgaagaatt
     2281 agaaagaaaa aaaatggaaa aacaagaatg tatgatatca ttcttgttta agaaaaaaaa
     2341 cattctattg aatccttata aatcattaga atttatgccg aaagaatcag gttttaatcg
     2401 agaaaattta gtggaaataa tcaagcaaaa ttattattgg ttttaattag tggagggtta
     2461 aacattgaaa aaagatatcg tcatagatat gtctgaagcc gaagttcgtg ctgcgctttt
     2521 ggaaaatgga caagtagtag aatttttctt tgaaagagca gccgaaaaac gattagcagg
     2581 aaatattttt aaagggatcg tagaaaatgt tctacccggt atacaatcgg cttttgttaa
     2641 tactggtttt gataaaaatg cttttttgtt tattggagat atcctgttgg atgagaaagg
     2701 gaaaataaag agcatagatc aactccttca aaccggaaag gaaattgttg ttcaggtagc
     2761 caaggaacca atgggaacta aaggggctcg agtgacgacc aaaatttctt taccagggag
     2821 atatgtggtt ttaatgcccg gaatgaataa tatgggtatt tcccatcgaa tttcttcttc
     2881 agaagaacga gataagctga agaaattaat tgaaaaaatt cgaccagcca acatgggagt
     2941 catagtgaga acagttgctg agggtaaaag taatgaagaa ataaaagaag acatcgaatc
     3001 actgcttcgt ttatggaacc gaattatgaa aaaagccgaa ctcatttcag ctccagcggt
     3061 tctctatgaa gaagcaagcc taatttatac gctaatacga gatgttgttg atgataattt
     3121 aggtgaaatt tggattaatt cctattttgg ttttcagaaa ttaaaagatt ttattgaatc
     3181 aatgattcct cgtttcgtcg accgtattca tttttttgat agtaaagaaa acattttcga
     3241 tcattttaat ttaaatcggg aattagaaaa agccttaagc cggaaagttt ggctcaaaag
     3301 cggagggtat ctggtttttg accgaactga agcaatgaca gtgattgatg tcaataccgg
     3361 aaaatatatt ggaaagaaag atctccaaga aactatttta aaaaccaatt tagaagcggc
     3421 ggaagaaatt gcccggcaag tacgactccg agatatcggg gggataattt taattgattt
     3481 tattgacatg gggaagaaag aacatcaaaa aatggttatg aatgtttttg agcaattttt
     3541 gtctcacgac cgaacccgtt ccagtttggt tcagatgagc gaactgggtt tggtggaaat
     3601 gactcgaaaa agagttagaa aaaacctgga tgccattctt tgccaaccat gtaattgttg
     3661 tcaaggtgaa gggagagttc tttcgatgga gactatcgct attaattttt taaggcgggt
     3721 tgaagaaatt tgtcgccatt ctcaatcaac gaccattggt tttactgtag gacaagagct
     3781 gggggattat ttaaaaaata atggaaaaat atttttaaat aatttaaaaa aggtatacag
     3841 taaagaatta gtatggaaaa tcgatccaaa tttatctcta cgtcgttatt cgattgttgg
     3901 agttggtgaa gacgggattc gagaatctat cgataaaata gaataatcat tgttagtccc
     3961 gtttgacaaa atccttcaat cttgatacag tcttaaccgt taaaaagcag ggctaaaacc
     4021 aagaatataa ttgtaataaa ggaataaata atctgaataa aagtatgact ttggaatgga
     4081 ggagatttgt aatgttcgct cttattgaag ccagtggaaa acaatatcca gttcaagaag
     4141 gttcggtcat tcaactggat agttatcaag gaaatttaaa agatgaagtg gtttttgaac
     4201 gagttttatt tattcgtaat caggaccaag ttgttgtcgg tcgcccctat gtggagggtg
     4261 ccaaagttaa aggtatcgtg ttaagaaatg ggaaatccaa aaaagttgtg gtttttaaat
     4321 ataaaccgaa ggtgaaatac cgacgtttaa ctggtcatcg tcaaccaatg actttaataa
     4381 aaattcaatc aattgaatcc taatccttga ggaggcttta ataactatgg cacataagaa
     4441 agcaggcgga agcgggaaaa atggacgcga tagtaacgct caaaggcttg gtgtaaaaaa
     4501 atttagtggt cagtatgttt tggctggcaa catccttatt cgacagcgag gaacaaagat
     4561 caaaccaggg ataaatgtgg ggacagccaa ggattatact ctttttgccc ttcaaagtgg
     4621 atatgttgtt tatcaagaga aagctgggag gaaatatgtc tcgattctcc ctgagatgcc
     4681 ttctctggat tcataattag tatcagttta ttaaaataag aaactctcct ttttattatc
     4741 caactcattt ttttattcag ccttggagaa tcgaatgatt gattcagtaa tttggggtgt
     4801 ttaattgttt gttgatcagg tcatattaaa agttcgtgct ggtcgtggtg gtgatggtgc
     4861 gatcagtttt ttaagagaaa aatatgtccc tcgaggtggg ccagcaggtg ggaatggcgg
     4921 aaaaggaggg aacgtaattg ctattgcatt atctcagaaa aaaactttat atgatgttgc
     4981 tattcagaaa gtctacgaag catcaaatgg tgaaccaggt aaggggaaaa atcaatttgg
     5041 aaaagatgga gctgacgtgg ttttagtagt accggttggc actcaaatta ttgatcaaaa
     5101 agataatcaa atcatagctg atttaactga agataagcaa gaagtcataa tagcccgagg
     5161 tgggcgagga ggacgaggga atagttcatt tgcaacatcg accaatcaag ctccccgcat
     5221 tgccgaaaaa ggagaattgg gtgaagcgag agagattcgc ttagaactta aattaattgc
     5281 tgatgtaggc attagtggtt ttcctaatgc tggaaaatca acactgctct cagtagtaag
     5341 cgctgcaaaa cctcgaattg ctgattatcc atttactact cttcatccga atttaggcgt
     5401 tgtgaatcat catgaccagc agtttattat ggtcgatgtt ccgggcatta ttgaaggtgc
     5461 cagccgtggt gctggtttgg gtttagaatt cttacgacac atagaaagga cacgtttgat
     5521 cctccattgt gtggatttat cccaattcta ttctgagaac aatccctatg acgatttttt
     5581 gactcttcgt cgggaatttg aagcttatag ctcttctttg gcagccaaac ctttttttgt
     5641 agtggccacc aagcttgacc ttatcaaagc tgctcaaaat tttaatgaat ttaaatcaac
     5701 tcttgagcag cagggtattc cggtttttgg ggtttcttct cttaccaagg aaggaatgaa
     5761 tcaactactc gatgcgatta tcgaattttt aaaaaatgcc cctgaaattt ttaccaaaga
     5821 aattctttct caatccaaaa ataccccatt gagtcctatt cgatcccgtc attatcgatt
     5881 ttcaacgcct tttcttgatc gtttaattgc ggagatcgat tggaattccg ataatgctct
     5941 ttcgattttt aaccaaaagt tggttcattt tgactttcta aaatattttt ctgccattca
     6001 acccggatca atagttgaaa tagaaaattt gaattttatc tggactggaa aacgccttga
     6061 agcccataaa accacttcca aatgatcatt ttaataaacc gatgaagcga attgatttta
     6121 ttgaaaacag ccgagtggtc gttataaaag tcgggagttc attgttggtt aaaaattttg
     6181 ttttagaaat tccccgatta gaggaattag ctcgaaacat tcatcaactc cgtcaaagtg
     6241 gcaaaaaagt cattttagtg acatcaggtg ctatggcttg tggaatgagt cgtttagggt
     6301 tgaaacgaaa aagaaacgat atcccgttca aacaagcaat ggcagcagta ggccaaggta
     6361 ttcttatgca gaattattgc caggtatttt ctcgaattgg tattccagtt gcccagatat
     6421 tgttagcacc ggaagacgtt cataatcgac ataaatatct caatgcccgt aatacctttc
     6481 aaaccattct cgatttcaat gttttgccga ttgttaacga aaacgacacc gttgccatag
     6541 cagaattaaa atttggtgat aatgatcgcc tatcagcctt ggtggcttca ctaatcaatg
     6601 cccagcttct tatcatcctt tctgacattg agggactgtt tacctctgat cccaaaaaaa
     6661 actccgaagc tatcctcata aaagaagttg agaggataga tgataaaatt gaaaggatgg
     6721 ctggccggga ggggagcaat atttcaactg gtgggatgaa aagtaaaatt atggctagtc
     6781 gtatggcgac cttttcgggt attggggtca tcttggcttt ggggaaggat ttttctatta
     6841 ttcaaagaat atttgctggc gatgaagttg gaactttctt ttaccctcag accaaggtcc
     6901 tcaaaaaccg gaagcgttgg attgcctttg gaatgatgac ccgaggtcgg gttatcatcg
     6961 atgagggtgc ttatttcgct ttaatgagaa ataaaagctt attaccagca gggatcaagg
     7021 gagtagaagg cgtttttgat tcaggtgact gcattgaagt tgtcaatgaa gaaggtcaat
     7081 cggttgccaa gggtttggtg aacttttcat cgacagagtt aaagaaaatc cgtggtttac
     7141 atactactga gttacaggaa gttttttttg atcaagagat aaatactgaa gtgattcatg
     7201 ttgataactt agtgttattc aatcgggagg agaaagacaa tggctgaaga tatgaaggtt
     7261 caggttcaga aagcaaaaaa ggctgctcag ttgctggcga cggtttcaac ccagaaaaaa
     7321 aatgattttc ttggggatct ggctcaacgt ttagaaggag aacaatctcg gattgagcag
     7381 gaaaaccaaa aagatgtaaa aattgctgta gaaaacaata tgaaatccgg gttcattgat
     7441 cgtttaatcc tcaataaaaa aagaattcaa ggaatggcag atggtttaag acaagtagcg
     7501 gctcttgctg atccggttgg ggaagttatg tcaggctgga agaggccgaa tggtttaatg
     7561 gtaaccaaag tgagagttcc actgggagtc ttggcgatta tttatgaagc tcgacccaat
     7621 gtgaccatcg atagcattgg attaggaata aaatctggga attccttagt tttaagagga
     7681 agtagttcca ccctcaattc gaaccgattg ctaatccttt tagcccgcga atctctccgt
     7741 caatattctc ttcctgaaga ttcggttcag ctcattgaaa gtaccagcca cgatgacatt
     7801 gctcagctgc tggccctgag agagtatatt gatgtagcga tcccccgagg aggtactggt
     7861 ttgattcata acgttgtgac tcattctcaa gtgccggtta ttgaaaccgg tgttgggaat
     7921 tgtcatgttt atgttgataa agaagccgat ttagggatgg cagaaaggat tgttatgaac
     7981 gcgaaaatcc aacgaccgag tgtttgtaat gctgtagaaa cattattaat tcaccaggat
     8041 attgccaaaa tatttctacc ccaagtctct atgtctctca aaaaagctgg agttgaaatt
     8101 cgaggctgtg agcaagctcg tcaaattctt cctgatttga ttccggcgac cgaggatgat
     8161 tggaaaaccg agtatctcga cttgatttta gcggttaagg tagttaaaac tctaaatgaa
     8221 gccatcgagc acatcaatac ttatggaacc cgccattctg aatcaataat taccgaaaat
     8281 tattcgaatg ccaagaattt tatggagaag gttgactcag cagcggttta tgtcaatgct
     8341 tcaactcgtt ttaccgatgg agagcaattt ggtctgggag cagaaatagg tattagtact
     8401 cagaaaattc atgcccgggg acccatggga ttgcaagaat taaccacaat aaaatacgtg
     8461 gtttatggaa ccgggcagat tcggaacgct taaaaaatta aaaaacaata tttgaaccag
     8521 atattccaag gagagatagg tttagatgaa aaaaggtatt atgggtggta cttttgatcc
     8581 gatccacttg ggacatttag ttactgctga agaagtgaga gattattttc atttagacga
     8641 ggtggttttt gttccttcag cacgaccacc ccataaggtt ggacaagtga taaccagtcc
     8701 cgagcatcgg tatttaatgg tggtattggc gactattacc aatccctatt ttcgagtttc
     8761 ccgagtggag ttggaacggg ttggaccttc ctatgcagtt gatacactgc gatattttcg
     8821 tgaattttgg ggagatgaaa gtgaaatcta ttttatcacc ggagccgatg cttttgcaca
     8881 gatttcaagc tggaacaatc ctgaggagct tttccgatta tgtacctttg tggcagcaac
     8941 tcgacctggc tataaaatgg tgagtatgga agagaaatat cgacaaaagg tgaaaatgat
     9001 cgaagtatct gctctggcca ttgcctcgtc ggaaattcgg cgacgggtca aaaagggaga
     9061 atcaattaaa tatttggttc ccgaagccgt tgaaaactat attaaaaaaa acgggcttta
     9121 tgtcaatgaa tgagtatatc accaagcagc ttaaaaatat gatttctccc tcacgattca
     9181 atcactcaat tcgagttgcc gaggaagcca gaacgctggc agttgatttt ggtgaagatc
     9241 cagaaaagtc ctatttagca ggtcttctcc atgattcagc aagagctctt gcttgtaagg
     9301 agatggttca atatatcccg cccttttttc ttccaattgg ctattctatt ccggctatat
     9361 ttcatgcttt tgcaggtccc cgatttattg ctgagcagtt tggcattaca gattattcaa
     9421 tacttcgtgc tgtttgctgg catgcaactg cctgtgaaga tatgagtaat tttgataaaa
     9481 tactttttgt ggctgattta tcggaaagtg gacgtcgatt ccaagaaagt gatacaattc
     9541 gtgaagcttt gcagagagga cttcaagtag cttttacaaa aactttagaa atgaaagtga
     9601 attatttact ttcaacaaag aaaattattt atccagccag cttggaagct tggaataaaa
     9661 ttattggaat atccatagag gaagcgaatg aaaagaagca gaagaaagaa aagttataaa
     9721 tatttatatt tagccattgg gtttattata ggcggaatcc ttttttttgg tctcaataat
     9781 cataatattc ggaataaatt ggtacaaacg gttatgggtt atgccccacc agaaagtacc
     9841 actattttag tagttggtca ggatagcatt aaacccttac ggagtgatac cattatttta
     9901 ctttgtctca acaccaagag tgaagaaatt cttttgtttt ccatcccccg cgatacccgc
     9961 attgaagtac cgggtaatgg atatgataaa gtcaatcatt cttatgccaa gggcgggatc
    10021 caacttctca aagaaacgat tgagaaaaac ttagacattc atattcctta tacagtagaa
    10081 atcaactatc aaggatttga aaaggttatt gacctgttgg gcggagtgga aattgacgta
    10141 gaaagtgata tgaaatacac tgatcgagct caggacttgg taatcaatat ttcagcaggc
    10201 aaacagcgcc tgaagggcga taaagcctta caatatgttc ggtatcgtaa tgataagttg
    10261 ggagatattg gtcgaatcaa aaggcagcaa aattttcttc aggctttatt tcatgagatt
    10321 gataatccct taaatcttcc taaaatgcca caaattatcc aagaattacg tcaagccttg
    10381 ataaccaatt tttccaatga agaacttatt aatttaggtc tttggtttaa ggacttaaat
    10441 gaaagagtga tgataaccga tatgatgcca ggggaagcag cctatataga gggggtaagt
    10501 tattgggaac cagatttaga cacatcaaaa aagttggtta ccgaattctt tctcaaagag
    10561 aaaagagtag aggaacagcc gaatgaatcc taaagaaaaa tgtttaatcg ttttggatgt
    10621 cgccgatgaa aagaaaatgc aggacgttgt aattcttgac ctgagggatg tttttcccgt
    10681 ggctgattat tgggttattg gcagtgctgc gacatttatc caaaccaaag ctattgccga
    10741 ggaaataaaa aagaccttgg actcctactc agttcaaccc tttcatattg agggattcga
    10801 tgttggtgaa tggattctta tggattacgg tgatgttatc gttcatattt ttcgagagga
    10861 agagagacac tattatctgt tggaaaagtt atggagaaat gctcatttgg tttattctaa
    10921 ggaattacag ataaataaga tcaacgaaat agattgacat cgtagtggag aatatgttat
    10981 tttacagctt cgggttctca tcatactatg agtttaatcc cagataaaaa aggtgaagag
    11041 aaact
//
LOCUS       DAOSOL010000006        10860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6036_length_10860_cov_4.774549, whole genome shotgun sequence.
ACCESSION   DAOSOL010000006 DAOSOL010000000
VERSION     DAOSOL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6036_length_10860_cov_4.774549"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PK171_00350"
     CDS             <1..234
                     /locus_tag="PK171_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOT04684.1"
                     /translation="LISVRSGDDFQAMLAMGIILSILMQVIVNMSAVLKILPVSGVPL
                     PLVSYGNSSILITMIEIGLLFNIARQSKVNHFE"
     gene            227..1309
                     /locus_tag="PK171_00355"
     CDS             227..1309
                     /locus_tag="PK171_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HOT04685.1"
                     /translation="MNEIFIAAGGTGGHIFPALSIAQHLSRDFPVRFIGTKRGMERKL
                     VPEAGFSLSFIRARGWNRTSAIDFLRAFGDNIVGLFQSGYLFIKFRPRAVLVFGSYVS
                     LLIAFWAKLFRVPIFVQEQNIHPGFANRIISRWSKKIFVPVDEAAEGFRDSNQIVIIG
                     NPLREEVLDWQGKDKEAKEKLGLDPQKKTLLIIGGSRGSHLINQAFWQALPFIEELKF
                     QVIHITGDESFQEAMKMAALYPFPYLIYEFYPNPGIFYSAADLAISRSGANVTYELFC
                     FGLPAVLIPFGGATEGHQLYNAQWLGKKQPVVIIDEIRLQPEGLAQAIRSLSELPAKK
                     YKKNEDFRFSAARIAGMIVKEIRRRK"
     gene            1311..2732
                     /gene="murC"
                     /locus_tag="PK171_00360"
     CDS             1311..2732
                     /gene="murC"
                     /locus_tag="PK171_00360"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT04686.1"
                     /translation="MNAHLVSLPNELYFIGIGGTGMSALATIAFEMGYQVSGSDIFSG
                     ENTCRLQEKGIPVFIGHSRERIKNISAVVISSAIPPENVELQEAIQRNIPIIHRGEML
                     AFFLNQKKGIAIAGTHGKTTTTSMISLLLEVAGLDPTVLVGGEVEDIGGNARFGNGEY
                     FISEADESDGSFLKLNPYCAVITNIEDDHLEYYGSQENELNAFISFANGVKEGGFMVA
                     CGDHPNVQVMFNQPLKTNLMTYGMINGVVDVRGSMIEERAEGSLFRVTHRNQDLGSFI
                     LNIPGIHNVINSLACIAVGIKLGIDKKLIASALEMFKGVKRRYERIGYVDGTLVVDDY
                     AHHPTEMGVVLKTALSRTLGKVIVIFQPHRYTRTKRLYREMAEILKKAHQVILFPIYS
                     AGEKPIEGITSNLIYDELKKAGYDGVHMVSTIDEALNITENLIGPGNILITMGAGDIW
                     KVADSMCRKNGNQLRKSTPVLRT"
     gene            2657..3592
                     /gene="murB"
                     /locus_tag="PK171_00365"
     CDS             2657..3592
                     /gene="murB"
                     /locus_tag="PK171_00365"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT04687.1"
                     /translation="MEGSRQHVQEKWKSIEKEYAGSPDLIVAPQAEMKFYTTWKIGGR
                     VIALVDVLRSVIFPNLFFKMQELDCPWKILGKGSNILVSDQGYSGVVIRLAGEYSEMN
                     YLGNHCIESGAGVALSHLVSFALGHSLGGFEFLVGVPGTVGGAVRINAGCFGQEISNL
                     IQEILVMDNNGNIEWLKREKINFFYRGSSLKKDRLTILKVIFQLFSDKSENIRNNVRR
                     YSLLRKQFQPVGWPSAGCVFKNPPGDYAAKIIDQMGFKGLRTGFAQISEKHSNFIINR
                     GSAKARDVIVLIDWIRQEVQRERNIFLENEIEVWQ"
     gene            3699..4304
                     /locus_tag="PK171_00370"
     CDS             3699..4304
                     /locus_tag="PK171_00370"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOT04688.1"
                     /translation="MNQIEVIGNRTLSVKYIANASGIQVGQSIFGLRSWEIENRIKKI
                     NDVKSVKVQKVFPHLIRIEIVERAPLAKVVVGDTWFYVDDQGVRVNTPSDNPDQLISL
                     FVPSLEYDSIPITLEVIRTWMSDFDTPLKLVKMVHNNLFILQLQNDIFIKCESVQNLK
                     EKVPVLKPLLREVQVKALKVVGFDLRMKKDIVLIQDEEEVF"
     gene            4301..5566
                     /gene="ftsA"
                     /locus_tag="PK171_00375"
     CDS             4301..5566
                     /gene="ftsA"
                     /locus_tag="PK171_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOT04689.1"
                     /translation="MKLFKYIETSNPTIAAVDVGTSKVCTLIGKIRSNGIEILGIGLS
                     ASSGIKKGKVVDVEKASHSIKESLLNSLSVAKEEPNFLYTGIAGDYVTSRNAENEILL
                     GKVSREVYEKDIEKVIEGTRAQIASDGQAVIHVIPQEFSLDDQRGIAHPRKLVGTRLK
                     VNAHVVTAEENHLHNLVECFQSVGYEVYRTVFQPYASALAVLTSGEQEAGTVLIDIGG
                     GTTDIAVFYNDSIYYSNILPVGGELITSDLAIGLRTSRDEAERLKLQYGSVYSKYVPD
                     DEMIEVKDISMKSLRSVKRKFACEIIEARVKEMILMIRREIRASGMSTVLRGGIVLTG
                     GSSLLGGLNEFASSLLNMPVRIGFPESKNYLGQVQVISSPIFSTSCGLLQLALTEGQE
                     RKTDHRFSEGSSGKIHGMVNKLKDFFMMG"
     gene            5585..6655
                     /gene="ftsZ"
                     /locus_tag="PK171_00380"
     CDS             5585..6655
                     /gene="ftsZ"
                     /locus_tag="PK171_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT04690.1"
                     /translation="MSKAKGNAGENNNVVIKVFGIGGGGGNAVNRMVKSGLMGVSFVA
                     LNTDAQVLSRSLAGVKLQIGSKLTRGLGAGANPEIGRRAAEEDRNKIYQVLENTDMVF
                     VTAGMGGGTGTGGAPVVANIARELGILTIAVVTKPFAFEGRKRNRQAEEGIEQLRKVV
                     DALIVIPNDRLLQVTEKTTSIVEAFQMVDDILLQGVQSISDLINVPGIINLDFADIRT
                     IMANAGTSLMGIGRASGENKATEAAKVAISSPLLETSFKGAKGILFNITSGPNLGILE
                     INKAAEIICSAASDETNIIFGAVIDKNIKDELKITVIATGFDHGGEKENEETQEIFQR
                     EVADSFTFDDLDVPAFMRKKMK"
     gene            6652..8232
                     /locus_tag="PK171_00385"
     CDS             6652..8232
                     /locus_tag="PK171_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT04691.1"
                     /translation="MILNITHLKNLERGLSLNNKMGKYLFVGCFPGPYEMGMANLGYQ
                     SVLKTVFDSPQWRVERLFTDTGIRTFEKSIPVAEADIVGLTLGFEIEIFSLVQLFMDS
                     GLEVYANKRAENQPLVLVGGPLASLNPEIIAPFADIVFIGESEESLPDLLTAWEEAQD
                     LDLSRQETLLYLSRFPGVYVPRFYFPMVKGSIFKGFEKVGGVPERIQKQRVDVSRFEV
                     FSHIYTSQSFFKNMGLMEINRGCSYRCRFCAGGAIYRPLRQRPIEMVMKMIDNLEKFT
                     SHLGIIGSDVLSHPQWEDIIKYAIKNAFTVNFSSLSAVTLSRRREYLSYLVKCGIKTL
                     TLAPESGDAETRQYFGKGLDDEEWTDLIQNIFQSGIPKVKLYFMIGKAFHSAEKDLDF
                     IHKLSRKINSKHQLSVSYSFLVPKPHTDLENMKSLSFLAWKKERELFETGLKKMKIRF
                     SGESLRVAWIELLLARADRFLAQEIPNLMKQKNGLVFNQWKTVLKKMGREFDEWPRHP
                     WEGDLYPWSIIDNHERRL"
     gene            8260..8964
                     /locus_tag="PK171_00390"
     CDS             8260..8964
                     /locus_tag="PK171_00390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT04692.1"
                     /translation="MMNRYIQKRLEIVQENILRSCQKSGRKSDEIKIIAVTKGIGVDQ
                     MQEAFSCGLNRFGENRIQEAEQKKISFRKEPIEWHFIGQLQRNKIPKVVEYFQYIHSL
                     DRINQFQTLEKRLEMSNEDCYPVMVQVNLTGKEIQAGLEKQDVVPFLESLHFYRHIRV
                     SGLMTIGPQGSLEEIRRVFRELYQIKEEVESKKYSWISPTLALSMGMSDDYPIAVEEG
                     ATYLRLGRCLFGERSY"
     gene            8965..9771
                     /gene="proC"
                     /locus_tag="PK171_00395"
     CDS             8965..9771
                     /gene="proC"
                     /locus_tag="PK171_00395"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOT04693.1"
                     /translation="MKQSILVVGCGNMGSALTRGLCLDKWHNKYDFCLYDKVLPKAIQ
                     IANELGLSYVADLNEVKSEPYLIFLAVKPNQVKLVCQDLTQFKNSILISLAAGVNQEQ
                     LQKYLGEGQPIVRMMPNLGVEVGEGVVALHFNSRVSEENREDLLFLFSSLGWVFEAEE
                     KEFDQITALSGSGPGLVAVFLEAMMDAGVNIGLPWEKSLKMVIQTMLGTATWLKKKNC
                     HPGLLKNMVCSPGGTTIAGIKRLELSGFRGIVMDGIETASKRAKNNMAEE"
     gene            9777..10331
                     /locus_tag="PK171_00400"
     CDS             9777..10331
                     /locus_tag="PK171_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOT04694.1"
                     /translation="MDLKPENILEIEFSRSFRGYNEDEVNEFIEEIAEAVEAVLKEKE
                     KLLQENEEYRRKVNELSQYAKRLEGNLDEWKKQLEIEKELTRRESQMHLKEAQIRGNR
                     IVDEALKKKKEIEVSYSELKEKYRLFQIRFKSLLQTFIDSIDWKEQPTDMNAPEPEKI
                     DQNPIHSPEDATSFSFRDLEDEQK"
     gene            10331..10834
                     /gene="lspA"
                     /locus_tag="PK171_00405"
     CDS             10331..10834
                     /gene="lspA"
                     /locus_tag="PK171_00405"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT04695.1"
                     /translation="MQFKGRNCPGSCFVIFLISCFGFLLLDRLTKWWALNNLTDRTIQ
                     IIPGFFDLTLRRNVGSAFGIRLLNPPAHLIITIAISIAFIYFMLRFLGNWKWWFFLGA
                     GLFLSGAWGNLIDRLRWGFVIDFFEPSIWATFNIADITIIAGLVIIFIQIWIQGSAIE
                     ETKNQDV"
BASE COUNT         3247 a         1820 c         2558 g         3235 t
ORIGIN      
        1 ctaatttcgg ttcgttcagg tgatgacttt caagccatgt tggctatggg aattattcta
       61 agtatcctca tgcaggtcat tgtaaatatg agcgcagtgc tcaaaatcct tccggtatca
      121 ggtgtacctt taccattagt aagttatggg aactcatcaa tactgattac tatgatcgaa
      181 atcggtttgt tgttcaatat cgcacggcaa tcaaaggtga atcattttga atgaaatatt
      241 tattgcagct ggtggaaccg gtggtcatat ctttccagcg ctcagcattg ctcaacattt
      301 atctcgtgac tttccggttc gctttattgg gaccaaaaga ggcatggaaa gaaaattggt
      361 tcccgaagcc ggtttttcac tttcttttat tcgtgctcga ggatggaacc gaacatcagc
      421 tatagatttt cttcgcgcct ttggcgataa tatagtagga ttgtttcaaa gtgggtatct
      481 ttttatcaaa tttcggcctc gggcggtctt ggtttttggc agttatgtct ctttactgat
      541 cgctttttgg gcgaaactat ttcgagttcc aatctttgtg caagaacaga atattcatcc
      601 gggttttgcc aaccggatca tctcccgttg gtcaaaaaaa atctttgttc cggtggacga
      661 agcagccgag ggctttcgag attcaaatca aattgttata atcggtaacc ccttacgtga
      721 agaagtgctt gattggcagg gaaaagataa agaagccaaa gaaaaactgg gtcttgatcc
      781 ccagaaaaaa accctgctta ttattggagg gagccgaggt tctcacctga tcaaccaagc
      841 attttggcaa gcactaccat tcatagaaga attgaagttt caagtcatcc atatcactgg
      901 agatgaatct tttcaagaag ccatgaagat ggcggcgctt tacccatttc cctatttgat
      961 ttacgagttt tatccaaacc cgggaatatt ttattcagca gccgacttag caatcagtcg
     1021 ttccggtgcc aatgtcacct atgaattgtt ttgttttggc cttccagccg tcctgatacc
     1081 ttttggagga gcaactgaag gccaccaatt atataacgcc caatggttgg ggaaaaagca
     1141 gccggtggtc attatcgatg aaataaggct tcaaccagaa ggcttggctc aggcgattcg
     1201 aagcctatca gagcttccag caaaaaaata taaaaaaaat gaagattttc gattttcagc
     1261 agcacgaatt gctggtatga ttgtcaaaga gatccgtagg aggaaatgaa ttgaacgctc
     1321 atctcgtctc attacctaac gaactctatt ttattggtat tggcggcact ggcatgagtg
     1381 ccttggcaac catcgctttt gaaatgggat atcaggttag tggttctgat attttttccg
     1441 gtgaaaacac ttgtcgactt caggaaaaag gcatcccggt atttatcggt cattcaagag
     1501 aaagaattaa aaatatatct gcagtggtta tttcctccgc catcccccca gaaaatgtcg
     1561 aactccaaga agctattcaa agaaacatac ccattattca ccgaggagag atgttagctt
     1621 tttttctcaa tcaaaaaaag ggcatagcta ttgcaggtac ccacggaaaa accaccacga
     1681 cctcgatgat ttcactgtta ttagaagtag ctggcctgga tccgacggta ttggtgggtg
     1741 gggaagttga agatattggg gggaatgctc gctttggtaa tggggagtat tttatatctg
     1801 aagctgacga gagcgacgga tcttttttaa agctcaatcc ctactgcgcg gttattacca
     1861 atatcgaaga tgaccatttg gaatattacg gaagccaaga aaatgaactg aatgctttta
     1921 tttcctttgc caatggcgtt aaagagggag gatttatggt agcctgcgga gatcatccca
     1981 atgtacaagt gatgtttaac caacccctca agaccaattt aatgacttat ggaatgatta
     2041 atggcgtagt tgatgttcgc ggatcgatga ttgaagaaag ggcagaaggt tccttgtttc
     2101 gagtgacgca tcgcaaccag gatttgggat cttttatttt gaatattcct gggattcata
     2161 atgtaattaa ttccttagcc tgcatagcgg ttgggatcaa gcttggtata gataaaaaat
     2221 taattgccag cgccttagaa atgtttaaag gagtaaaaag gcgttatgaa cgaattggtt
     2281 atgttgacgg aaccctggtt gttgacgatt atgctcacca tccaactgaa atgggtgtag
     2341 tgttaaaaac agcgttgagc cggaccttag ggaaagtgat cgttattttt caacctcacc
     2401 ggtatactag aaccaaacgt ctttatagag aaatggccga gatattaaag aaagcccatc
     2461 aagttatcct ctttcccatt tattcggcag gcgaaaagcc gattgaaggt attacctcta
     2521 atttaattta tgatgaattg aagaaagccg gttatgatgg tgttcatatg gtttcaacca
     2581 tagacgaagc tcttaacatt accgagaacc tgataggtcc aggaaatatt ctgataacca
     2641 tgggggccgg agatatatgg aaggtagccg acagcatgtg caggaaaaat ggaaatcaat
     2701 tgagaaagag tacgccggtt ctccggacct gattgttgca ccacaggctg aaatgaagtt
     2761 ttataccacc tggaaaattg gtggccgggt tatagcttta gttgatgttt taaggagtgt
     2821 cattttcccc aatttatttt ttaaaatgca agaactggat tgtccctgga aaattctggg
     2881 aaaaggatca aatattctgg taagcgacca aggttattct ggtgtagtta ttcgtcttgc
     2941 tggtgagtat tccgaaatga attatctcgg aaaccattgc attgaaagtg gtgctggtgt
     3001 tgctctttct catttagtaa gttttgctct tggccacagt ttaggaggat ttgaattttt
     3061 agttggtgtt cctggaacgg tggggggggc ggtacggatt aatgccggtt gttttggaca
     3121 ggaaatcagc aatttaattc aagaaatctt agtcatggat aataatggta atatagagtg
     3181 gttgaagaga gagaagatta atttttttta tcgaggatca tcattgaaga aagataggtt
     3241 gacgattttg aaagtcattt ttcaactttt ttctgataaa tcagaaaaca ttagaaataa
     3301 tgttcgtcga tatagccttc tccgcaaaca atttcagccg gtaggttggc catcggccgg
     3361 ttgtgtattt aaaaatccac ccggtgacta tgcggctaaa atcattgatc aaatgggatt
     3421 taaagggcta agaaccggtt tcgctcaaat ttctgagaaa cattccaatt ttattatcaa
     3481 tcgtggatca gcgaaagcac gtgatgtaat agtactgata gattggattc gccaagaagt
     3541 tcaacgagaa agaaatattt ttttggaaaa tgaaatcgag gtatggcaat gaaatattat
     3601 aagtcacagt tttttttaaa actctttttt tttatcttgt tctaggagtt atcgcctggt
     3661 tggtttttag cctcttcatg agagggaatc tatttgcagt gaaccagatc gaggtgatcg
     3721 gtaatcggac tctatcggta aaatacatcg caaatgcctc gggtattcaa gttggacaga
     3781 gtatttttgg tttaagaagt tgggaaattg aaaaccggat caaaaaaatt aacgacgtaa
     3841 aaagtgtaaa agttcaaaaa gtttttcctc atttaattcg aatcgaaatt gtggaaagag
     3901 ctcccttagc aaaagttgtg gttggagata cctggtttta tgtagatgat caaggggtga
     3961 gagtgaatac tccatcggat aatcccgatc aattgatttc tctttttgta ccatccctcg
     4021 aatatgattc gattccaata actttagagg ttattcgcac ttggatgagc gattttgata
     4081 cacctttgaa gctggtaaaa atggttcata ataatctgtt tattttacaa cttcaaaatg
     4141 atatatttat caagtgtgag agtgttcaaa atttaaaaga aaaagtcccg gttttgaaac
     4201 ccttgttgag agaagttcag gtaaaagctc tcaaagtggt cggttttgat ctgcgcatga
     4261 aaaaggacat tgttcttatc caagacgagg aggaagtttt ttgaaattat ttaaatatat
     4321 tgagacatca aatccaacta ttgcagcggt tgatgttgga accagcaagg tctgtacgtt
     4381 aatcgggaag attcgatcca atggaatcga gatattaggt attggattaa gtgcttcgtc
     4441 tgggataaaa aaaggaaaag ttgttgatgt tgaaaaggcc tcccattcga taaaagagtc
     4501 tcttctcaat tccttgagtg tagccaaaga agagccgaat tttttatata ctgggattgc
     4561 tggtgattat gtcacttcca ggaatgctga aaatgaaata cttcttggaa aagtcagtcg
     4621 ggaggtatat gaaaaagata ttgaaaaagt cattgaggga actcgagccc agatagccag
     4681 cgatggtcag gcggttattc atgttatccc tcaggagttt tcgttagacg atcagcgagg
     4741 gatcgctcat ccacgtaaac tggtggggac tcgtttaaaa gtgaatgccc atgtagtcac
     4801 cgccgaggag aaccatctcc acaatttagt tgaatgcttt caaagtgtgg gatatgaggt
     4861 ttatcgaacg gtatttcaac cctatgcctc ggctttggca gtattgacct ctggtgagca
     4921 ggaagcagga acggttttaa tagatatcgg cggaggaaca accgatattg cggtattcta
     4981 caatgattca atctattatt ccaatatctt accggttggg ggagagctga ttacatctga
     5041 tttggcaatt ggtctccgga catcccgcga cgaggcagaa agattaaaac tccaatatgg
     5101 aagcgtttat agtaaatatg tgcctgatga tgaaatgatt gaagtcaaag atattagcat
     5161 gaagtctctg cggtcggtaa agaggaagtt tgcctgtgaa ataattgaag ctcgagtgaa
     5221 ggaaatgatt ttaatgatta gaagggaaat tcgagcctca ggaatgtcga cggtattacg
     5281 gggagggatt gttctcactg gcggttcttc ccttttggga gggctcaatg aatttgcctc
     5341 atcgctctta aatatgccgg ttcggattgg atttccggaa agtaaaaatt acttgggaca
     5401 ggtgcaggta atatccagtc caattttttc aacttcttgt gggctattgc aattggccct
     5461 tactgaaggc caggaaagaa aaacagatca tcgttttagc gagggatctt ctggaaaaat
     5521 tcatggaatg gttaataaat taaaggattt cttcatgatg ggataattac tgggggttac
     5581 tgagatgtct aaggcgaaag gaaatgccgg agaaaataac aatgttgtga tcaaggtttt
     5641 cggtattgga ggcggtggag ggaatgccgt aaaccgaatg gtcaagtcag gattaatggg
     5701 tgtatcattt gttgcactca ataccgatgc ccaggtgtta tcacgttctc tggctggtgt
     5761 taagctccaa atcggttcga aattaactcg tggtcttgga gctggtgcca acccggaaat
     5821 tggtcggaga gctgcagaag aagatagaaa taaaatctac caggtattag aaaacaccga
     5881 tatggttttt gtaacggctg gtatgggtgg tggaaccgga accggtggag ctcccgtggt
     5941 agcgaatatt gctcgcgagt tgggaatatt aacgatagcc gttgtcacaa aaccttttgc
     6001 ttttgaagga cggaaaagaa accgtcaagc cgaagagggg atagagcagc tcagaaaagt
     6061 ggtggatgct ctgattgtta ttcccaatga tcgcttactt caagttaccg agaaaaccac
     6121 ctcgattgtc gaagcttttc agatggtaga tgatattctt ctccaagggg tacagagcat
     6181 ttctgattta atcaatgttc caggcattat taacttagat tttgctgata tccggacgat
     6241 aatggccaat gccggtactt ctttaatggg tatcggtagg gccagtggag aaaataaggc
     6301 aactgaagca gcaaaagtag caatttccag tccacttttg gaaacatctt ttaagggagc
     6361 caaaggcata ctatttaata taaccagtgg accaaatttg ggaatcttgg aaataaataa
     6421 agcagcggag attatttgca gtgctgcttc ggatgaaacc aacatcattt ttggcgctgt
     6481 tattgataaa aatataaaag atgagttaaa aattacagtt attgccactg gatttgatca
     6541 cggtggagaa aaggaaaacg aggagacgca ggagatattc caaagggagg tagctgattc
     6601 ttttaccttc gatgatctgg atgtacctgc ttttatgaga aaaaaaatga aatgattttg
     6661 aatattaccc atttgaagaa cctggaacgg ggtttgtccc tcaacaataa gatggggaag
     6721 tatctttttg ttgggtgttt tcctggccct tatgaaatgg gaatggcgaa tctcgggtat
     6781 caatcggttt taaagaccgt ctttgatagc cctcaatgga gggtcgaaag attatttacc
     6841 gacactggga ttcgaacttt cgagaaaagt attccggtag ctgaagctga cattgttggg
     6901 ttgactcttg gttttgaaat tgaaattttc tcattagttc agttgtttat ggattcaggg
     6961 ttggaggttt atgctaataa gcgggcagaa aaccaacctt tggtcttagt gggtggtccg
     7021 ctggcttcct tgaatccgga gattattgca ccttttgctg atatcgtttt tattggtgaa
     7081 agtgaagaga gccttcccga tttattgact gcttgggaag aagcacaaga cctcgactta
     7141 agccgccaag aaacccttct ttatttaagc cgttttcccg gtgtctatgt tccgcggttt
     7201 tattttccca tggttaaggg ctcaattttt aaaggctttg aaaaagtcgg cggtgttcca
     7261 gagcggattc aaaaacaaag ggttgatgta agccgctttg aggtcttttc tcacatttat
     7321 acttctcaat cattttttaa aaatatggga ttgatggaaa tcaaccgggg gtgcagttat
     7381 cgctgtcgtt tttgtgctgg cggggctatc tatcgccctc tgcgtcagcg accaattgaa
     7441 atggttatga agatgatcga taatttagaa aaatttactt cacatctggg aattatcggg
     7501 tcggatgtgc tttcccatcc ccaatgggag gatatcataa aatatgcaat aaaaaacgcc
     7561 ttcacagtaa atttttcctc tctctctgcg gttactctct ctcgtcgtag ggaatatctt
     7621 tcttatttgg taaaatgtgg aataaaaacc cttaccctgg cgccagaaag cggagatgcc
     7681 gagactcgcc aatattttgg gaaaggtctg gatgacgagg agtggacgga tctcatccaa
     7741 aatattttcc agtcgggaat accgaaagtg aagctctatt ttatgatcgg gaaggctttt
     7801 cattctgctg aaaaggattt ggattttatt cataaattaa gtcgtaaaat taattcaaaa
     7861 catcaattat cagtttctta cagtttttta gttccaaaac ctcataccga tttggagaat
     7921 atgaagtctc tttcattctt agcttggaag aaagagagag aactttttga gactgggctg
     7981 aaaaaaatga aaattcgctt ttcaggtgaa agtctacggg ttgcttggat agagctcctt
     8041 ttggctcgag ctgatcgttt cttggctcag gagataccaa atctgatgaa gcaaaaaaat
     8101 ggtttggttt ttaatcaatg gaaaacggta ttgaaaaaaa tgggacgaga atttgacgaa
     8161 tggccaagac atccctggga aggcgattta tacccctggt caataattga taaccatgag
     8221 agaaggttgt gagggaaatg aaggggaacc ggatcgaaaa tgatgaatag atacattcaa
     8281 aaacgattag aaattgtaca ggaaaatatt ttacgttcct gccaaaaatc aggtagaaaa
     8341 agcgatgaga ttaaaatcat tgccgtaacc aaagggattg gtgtcgatca aatgcaagaa
     8401 gctttttcct gcggattgaa tcgttttggt gaaaatcgaa ttcaagaagc cgagcaaaag
     8461 aaaatttctt ttcgaaaaga accaatagag tggcatttta ttggacagct ccagaggaac
     8521 aaaattccaa aagtggtaga atattttcag tatattcatt ctttagaccg gataaatcag
     8581 tttcaaacct tagaaaaacg cttggaaatg tcaaatgagg attgttatcc ggttatggta
     8641 caggtgaatt tgactggcaa agaaatccaa gctgggcttg agaaacaaga tgtcgtccct
     8701 tttttagaat ctttgcactt ttatcgacat attcgagtca gtgggttaat gaccattggg
     8761 cctcaaggaa gtcttgaaga aattcgtcga gtttttcgtg agctttatca aatcaaggaa
     8821 gaagtagaga gtaaaaagta tagttggatt tctccaactt tggctttatc aatgggaatg
     8881 agtgatgatt atccaatagc ggttgaagaa ggtgcaacct atcttcggtt aggacgttgt
     8941 ctatttggag aaaggagcta ttaggtgaaa caatccattt tagtggtagg ttgtggtaat
     9001 atggggagtg ctctgacacg aggactttgt cttgacaaat ggcataacaa gtatgatttc
     9061 tgcctttatg ataaagtatt acctaaggcg atacagatag ctaatgagct cggtttatct
     9121 tatgtagccg atttaaatga ggtgaagtcg gaaccttacc tcattttttt agcggtgaag
     9181 cccaatcaag tcaaattggt ttgtcaagac cttactcaat ttaaaaactc aatactcatt
     9241 tccctggcag cgggagttaa tcaggagcaa ttgcagaaat acttaggaga aggtcaacct
     9301 attgtcagaa tgatgccgaa cttgggcgta gaggttggag agggcgttgt tgcccttcat
     9361 tttaactccc gggtgagcga agaaaatcgc gaggaccttt tatttctatt ttcttcgctg
     9421 ggttgggtat ttgaagctga agagaaagaa tttgatcaga ttactgcctt gagtgggagc
     9481 gggccggggt tagtggcggt ttttttagag gcaatgatgg atgctggtgt gaacattggt
     9541 ttaccgtggg aaaaaagttt aaaaatggta attcaaacca tgttaggaac agcaacctgg
     9601 ttgaagaaaa aaaattgtca tcctggttta ttaaaaaata tggtttgttc tcctggggga
     9661 acaaccattg ccggaataaa acgactggag ttgtcaggtt ttagaggtat tgttatggac
     9721 ggaattgaga ctgccagtaa gcgagcaaaa aataacatgg ctgaggagtg acggaaatgg
     9781 acttgaaacc ggaaaatatt ttagaaattg aattcagtcg ttctttccgt gggtataatg
     9841 aagatgaagt aaacgaattt attgaggaaa ttgcagaagc ggttgaagct gtactaaaag
     9901 aaaaggaaaa attattacag gagaatgaag aatataggcg gaaagtgaac gaactgtccc
     9961 aatatgcaaa acgcttagag ggaaacttag acgaatggaa aaaacaatta gaaattgaga
    10021 aggaactgac tcgtcgagaa tcacaaatgc atttaaagga agctcagata cgtgggaatc
    10081 gaattgttga tgaagctttg aaaaaaaaga aagaaatcga ggtcagttac tcggagctca
    10141 aagaaaaata tcggttgttt cagattcgtt ttaaatctct tcttcagact tttattgaca
    10201 gcattgactg gaaagagcaa ccaaccgata tgaacgcccc tgaaccggaa aaaatcgatc
    10261 aaaacccgat tcattctccc gaagatgcca cctcattttc ttttcgggat ttggaagatg
    10321 aacaaaagtg atgcaattta aaggaagaaa ttgtcccggg agttgtttcg ttatttttct
    10381 aatcagttgt ttcggttttt tattgttaga tcggttaacc aaatggtggg cgctgaataa
    10441 tttaaccgac cgaaccatcc aaatcattcc tggttttttt gatctcactc tgcgaaggaa
    10501 tgttggaagc gcttttggaa ttcgcctttt aaatccacct gcccatttaa ttattaccat
    10561 agcgataagc attgctttta tttatttcat gctccgattt cttggcaact ggaaatggtg
    10621 gtttttttta ggagccggtt tgtttttatc cggagcttgg gggaacctca ttgatcgttt
    10681 acgttgggga tttgtaattg acttttttga accatcaata tgggcaactt ttaatattgc
    10741 cgatataacg atcatcgctg gtttagtaat aatttttatt caaatctgga tccagggttc
    10801 agcaattgaa gaaacaaaaa atcaagatgt atgataatga gttctgccgg attgatatct
//
LOCUS       DAOSOL010000007        10068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6685_length_10068_cov_4.044342, whole genome shotgun sequence.
ACCESSION   DAOSOL010000007 DAOSOL010000000
VERSION     DAOSOL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6685_length_10068_cov_4.044342"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PK171_00410"
     CDS             <1..677
                     /locus_tag="PK171_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04696.1"
                     /translation="YILYRDLRSYGLQEKYYREARNRGVVFVRFEDDDPPEVVQQGSL
                     LQVKVNSSVFQESVTLPAGLVVLSAGIVPLETNVAIGKMLKVPLNQDGFFLEAHVKLR
                     PVDFATDGVYVCGLAHGPKSALESMLQAKACVARAMNILSKDQIQSEAQIAYVLKERC
                     TACGDCEKVCAYKAVKINQEKKIAEVNAALCKGCGLCSATCKSTAIKVQGFAPEQLIS
                     EVEYLL"
     gene            674..1111
                     /locus_tag="PK171_00415"
     CDS             674..1111
                     /locus_tag="PK171_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOT04697.1"
                     /translation="MIESSWEPKIVAFLCHWCSYAGADLAGVSRMQYPPNIRVIRVPC
                     SGAINPLYIFKALREGADGVLVSGCHPGDCHYISGNYYARRKFYILKELLVWAGIEAD
                     RIHFSWVSASEGQKFSEVVTEVVNAVKPLGPSRKLVKEFSTDV"
     gene            1104..2048
                     /locus_tag="PK171_00420"
     CDS             1104..2048
                     /locus_tag="PK171_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT04698.1"
                     /translation="MFESILIDLAKKLLREKTVDLIIGYGSTHYPFRTEPIVINQEEQ
                     ADALVWNPFCGNNLARYLKYYKNTQHKIALMVKGCDSRAVQVLLKEDQIKRDRLYLIG
                     LSCPGLVNREKLQKQFPQASPEDFEWKETGWFYRGEQIDPELLLEETCLRCRYPNPPE
                     YNQLIGKPQTTSPYQTYQKDYLDPIDNLSSEERWDRWSKEMRKCIRCYACRNSCPLCY
                     CQECFADSTQPQWVSPAPTPSDNFLFHLVRTMHMAGRCVECGACERACPVSIPIAQLP
                     LKVEAIIREQFQFEAGTDQESAAPLLTYRENDPGDFVK"
     gene            2059..3057
                     /locus_tag="PK171_00425"
     CDS             2059..3057
                     /locus_tag="PK171_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOT04699.1"
                     /translation="MKSGKITKNDLIQWLKIHQKNFLVTERVAHHQPELLCDQSKEIK
                     NIKEALFLPWDKLFNYHWENQKWNVENIQPSASPRFLMALPCETRAIYEVLDRVFLQP
                     SETESGYAARRQGLKMVIVGCVNPEPTCFCHLVGGNPYWSHPDALFILPFHDEYYLET
                     NLPEQFDIMEKGTTLNKAEKEEIEVLRKQMEEKANQEKLPEDVPQGLYDLFEDQEWED
                     LSWKCLNCGACTFLCPTCQCFDMSAEGRLKGFQLKTWDSCMFPKFTLHASGHNPRPTF
                     KERVRQRVLHKFSYFPLREEGLYGCVGCGKCIEICPVNWNIREAVERMVKKIGNRS"
     gene            3041..3883
                     /locus_tag="PK171_00430"
     CDS             3041..3883
                     /locus_tag="PK171_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOT04700.1"
                     /translation="MAIDLENKNIYLPRLYQIMEIIPETSDIKTFRLDLEGENSSHLP
                     GQFAELFVPGVGEAPISITSSPTQKGILEFSIKRTGLVTSAIHRLNPNDRLGIRGPYG
                     NTFPLSDLTGQNLLFIAGGIGLAPLRSLINYVLDTEKRNHFKKVTILYGARSPQDLVF
                     KWELKKWQNREDITFLLTVDRGDDQWKGTVGLVPNVLKENVQVNPLEWKSIICGPPIM
                     IKFTIKALLEMGFQPPDIITTLEMRMKCGLGKCGRCNIGPYYVCKDGPVFTYQQLQNM
                     PEEY"
     gene            3897..5231
                     /locus_tag="PK171_00435"
     CDS             3897..5231
                     /locus_tag="PK171_00435"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT04701.1"
                     /translation="MNFQESIQALYSLINYEKTDFSYTDLKLDRMREFMTLLEHPDTH
                     SPVILVAGTKGKGSTCYYLERILASQQKKCGLYIKPHLLTFRERLRFNGEIIQPEELA
                     QLVTDIFPVINHMEKYSLYGKPTYFEVSVALAFQYFKKRKADYSIMEVGLGGRLDATN
                     VANPILTVITPISYDHTEILGETIPQIAQEKAGILRPYIPLILGLQEKKSAKTTIMQV
                     VENLRVPVFPLEDYYTYRIRSRSANGSVFELLAKSGREGIFSLPLLGDQQVENFLTAL
                     LSAEHLGYLPSNQQLQNLLNEAVWPGRIQVIGSSPLTIFDVAHNQASFATLCHTLRDY
                     LGIKKAVFLLGFLNGKDFPGIAEELFQMESRVILTTPFNPKAALPADIVHFFAPLIPH
                     HEVINDPYQAWEKALQYATEFHLPLVVAGSFYLAKLLSDKLNVTLSKEEVEL"
     gene            5228..6028
                     /locus_tag="PK171_00440"
     CDS             5228..6028
                     /locus_tag="PK171_00440"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT04702.1"
                     /translation="MIIVAERINATRKAIREALEKKDQEFFIQEAQKQEKAGAAFIDV
                     NAGTEATKEVADLKWLVESIQDAVEIPLCLDSANDQALEEALTVYNKKEVMINSFTAE
                     ENRIKALLPIVKKYDALVVGLAMGEGGIPQSVEERMKLVETLVKAVDEYSIPREHLFI
                     DPLVVPIGTDQNQGKLFLETLQAIPQTYPGVRTVCGLSNISFGMPNRRLINRSFLALS
                     IGFGLHAAIIDPLDDKLMGILFASEALMGRDEYCMNYLTAFRAGRLNE"
     gene            6238..6615
                     /locus_tag="PK171_00445"
     CDS             6238..6615
                     /locus_tag="PK171_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04703.1"
                     /translation="MFSIQLTKAKEFRRYIEDHYEFGDFALIRGREETAEIGFVFADE
                     DVNNWPSLYKKAENICDHFDKRLQEEGLKTVAYSRVGKDLDFITVSIVIRLHAFPEDQ
                     IHRIADVIMNILREVNPYHENEN"
     gene            6634..6891
                     /gene="purS"
                     /locus_tag="PK171_00450"
     CDS             6634..6891
                     /gene="purS"
                     /locus_tag="PK171_00450"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOT04704.1"
                     /translation="MKFRGKIVITLKPGVFDPQGVTIKSALHSLGYQETDEVKTGKYF
                     EINLDSSNLDTAQKRLEEMCDQLLANPVIENFHFTVEPSEN"
     gene            6902..7609
                     /gene="purQ"
                     /locus_tag="PK171_00455"
     CDS             6902..7609
                     /gene="purQ"
                     /locus_tag="PK171_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOT04705.1"
                     /translation="MKFGIVVFPGSNCDYDCQHVLSVVLSQETEMLWHESHDLKECDC
                     IVLPGGFSYGDYLRTGAVARFSPVMESIRNFIEEGGLVVGICNGFQILVESQLLPGAL
                     LPNQKGHFICRHVHLRVENTDTPFTRLFYPGEVISIPIAHHQGNYFIPHSSLSNLQKN
                     RQIIFRYCDIDGQINHATNPNGSIDSIAGIISSSQNVLGMMPHPERSSEAVLGSQDGK
                     RIFLSIIQWWEEKHHAK"
     gene            7599..9797
                     /gene="purL"
                     /locus_tag="PK171_00460"
     CDS             7599..9797
                     /gene="purL"
                     /locus_tag="PK171_00460"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOT04706.1"
                     /translation="MQNNEQIQVARELGLRDEEYQRILDILQRPPTPTELAMFSVEWS
                     EHCGYPHSRRWFELFPREGKFPVLVGEDAGGIVYQGQAVVFKMESHNHPSQVEPRQGA
                     ATGIGGIIRDIFGSGARPIACLDSLHFGPLDNARSNFIFKGVVDGIAFYGNCVGVPTV
                     AGELYFHPSYQGNCLVNVMSIGIASQDRLAKSWAKGKGNLIIYAGNRTGRDGIGGCSI
                     LASQEFSEQDEKRPSVQIGDPFTEKCLIEATMEALATGFLVGIKDMGAAGLTCSSSEM
                     AAAGKSGVKIYLDRIPVREEAMEPWEIMMSESQERMLLCVKRGYEDSVLQIFHKWGLE
                     AVVVGEVVDGDNITIEFNGKMVADIPAQELTKPPVYTPPAEKPAYLERINHYDWNDLP
                     LPNNWNEILLKLMASPNLCSRHSVFEQYDHMVQINTSMLPGTGTVILRVKNLPWGIAV
                     TTDCNPVFCYLDPYRGSQIAVAEAARNLAACGAQPAGITDCLNFGNPQKPDRYWQFIE
                     AIKGISDACKFFDLPVVSGNVSFYNESPTGPIHPTPTIGMIGIIEDVQKAVNAAFKDF
                     DDVIVLLGETRNEIGGSEYLRVIHQQEFGPAPQIDLSAEKNLQEFLIDAVRQGFVQSS
                     SDLSEGGLACALGEGCICGQEKKGCLIDIGNLPNIRLDALLFGESCGRALITCKPENF
                     ATLEKMARMKTIPFFVIGKVTRESLNIYNNQQSLISVSIEDLWKAWYHTL"
     gene            9811..>10068
                     /locus_tag="PK171_00465"
     CDS             9811..>10068
                     /locus_tag="PK171_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04707.1"
                     /translation="MIYHTHQWVYNINIHTGNWYQIRMKILIQESFPPLAKEVRGFEI
                     IGKKQILLNLLLSKKKGIKEIEFMRLPRHFVPRNDDSEFLLP"
BASE COUNT         2888 a         2098 c         2179 g         2903 t
ORIGIN      
        1 tgtatatcct ctatcgagac ctgagaagtt acggtcttca ggaaaaatac tatcgggaag
       61 cacgaaaccg aggtgtggtt tttgttcgct ttgaggatga tgaccccccc gaagttgtac
      121 agcaaggttc tcttctccaa gtaaaggtca atagttcggt gtttcaggaa agtgtaacgc
      181 ttccagctgg tttggtagtg cttagcgccg ggattgttcc tcttgaaacc aacgttgcca
      241 tcggtaaaat gttgaaggtg cctcttaatc aggatggatt tttcttggaa gcccatgtta
      301 agctacgacc ggtggatttt gccactgatg gtgtgtatgt ttgtggtttg gctcatggcc
      361 caaaatctgc tctggaaagc atgttgcagg ctaaagcttg tgtagcccga gcgatgaata
      421 ttttaagcaa agatcaaatt caatcggaag cccaaatagc ctatgtattg aaagaacgct
      481 gtacagcctg tggcgattgt gaaaaagtat gtgcctataa ggcagtaaag attaatcaag
      541 aaaagaagat agccgaggtt aatgccgctc tttgtaaggg atgtggtcta tgtagtgcaa
      601 cttgcaagag taccgctatc aaagttcagg gatttgctcc cgaacagctt atatctgagg
      661 tggaatattt attatgatcg aatcgtcttg ggaaccaaaa attgttgctt ttctttgtca
      721 ttggtgtagc tatgctggag ccgatttagc cggggtgagt cgaatgcaat atcctcctaa
      781 tattcgggtg attcgtgttc cctgttctgg agctattaat cctctctata tttttaaagc
      841 tcttcgggaa ggagccgatg gggttctggt ttccggttgt catcctggtg attgtcatta
      901 catcagtggt aattactatg cccgccgtaa gttttacatt cttaaggaac tgctggtctg
      961 ggcgggaatc gaagctgatc gtatccattt ttcctgggtt tcagcttctg aaggacaaaa
     1021 attctccgag gtggtcaccg aggtcgtcaa tgctgtcaag ccactcggtc cctctcgaaa
     1081 attagtaaag gagttttcaa ccgatgtttg aaagcatcct catagattta gccaaaaaat
     1141 tactccggga aaaaacggtt gatttaataa ttggttatgg ctccactcat tatccctttc
     1201 gaaccgaacc tatcgttata aaccaagaag agcaggctga tgccttggtt tggaatcctt
     1261 tttgtggaaa taacttagcc cgttatttga aatattataa aaatacccag cataaaatcg
     1321 ctttgatggt caaaggttgt gattcacggg ctgttcaggt tctcctaaaa gaggatcaaa
     1381 tcaaaaggga tcgactttat ttgattgggt tatcttgccc aggtctggtc aaccgggaaa
     1441 agcttcaaaa gcaatttcct caagcttctc ctgaagattt cgaatggaaa gaaactggtt
     1501 ggttctaccg cggcgagcaa attgacccgg aattacttct tgaggaaacc tgccttcgct
     1561 gtcgatatcc caatcctcct gaatataacc agcttatcgg taaaccccaa acaaccagtc
     1621 cttatcagac ctatcaaaaa gattacttag atccaatcga taacctttct tcagaagaac
     1681 ggtgggatcg gtggtcgaag gaaatgagga aatgtattcg ttgttatgcc tgtcgaaact
     1741 cttgtcctct ctgttactgt caggaatgtt ttgccgatag tactcaaccc caatgggtaa
     1801 gtccggctcc aactccttcg gataatttcc ttttccactt agttcggact atgcatatgg
     1861 ctggacgttg tgttgaatgt ggtgcttgtg aaagagcctg tccggtttcc attcccattg
     1921 cccaacttcc acttaaagtt gaggcgatca ttcgtgaaca atttcaattt gaagccggaa
     1981 ccgatcaaga gagcgctgca cctttgctca cttaccggga aaatgatccc ggagactttg
     2041 taaagtgagg taatggttat gaaatccgga aaaattacca aaaatgatct cattcagtgg
     2101 ttaaaaatcc atcaaaaaaa ctttttagtc accgaaaggg tagctcatca ccaacctgag
     2161 cttttatgcg atcaatcaaa agaaataaaa aatatcaaag aagccctttt tctcccctgg
     2221 gacaagcttt tcaattatca ttgggaaaat caaaaatgga acgttgagaa tatccagcca
     2281 tcagcttcac cccgatttct gatggctctt ccctgtgaaa cccgagccat atatgaagtt
     2341 cttgatcgag tctttctcca accatcggaa actgaaagtg gatacgcggc tcgtcgccaa
     2401 ggactaaaaa tggtcatcgt aggatgtgtg aatcccgagc caacctgttt ttgtcatttg
     2461 gtaggtggaa atccttattg gtcccatcct gatgcgctct ttattcttcc ctttcacgat
     2521 gaatattatc tcgaaaccaa cctaccagag caatttgata tcatggaaaa aggaaccaca
     2581 ctgaataaag ctgaaaagga agaaatcgaa gttttacgaa aacagatgga agagaaagcc
     2641 aatcaggaga aacttcctga agatgtcccc caaggacttt atgacctctt tgaagaccaa
     2701 gaatgggaag acttgagctg gaaatgcttg aattgcggag cttgcacttt tctgtgccca
     2761 acctgtcaat gctttgatat gagtgccgag ggacgtttga aaggttttca actcaaaact
     2821 tgggattcat gcatgtttcc caagttcacc cttcatgcct cgggacataa tccccgacct
     2881 accttcaaag aaagagttcg acagagagta ctccataaat tttcctattt tcctctccgg
     2941 gaagaaggac tttatggatg tgttggatgt ggaaaatgca tcgaaatttg cccggtgaat
     3001 tggaacattc gggaagctgt tgaaaggatg gtaaaaaaaa ttggcaatcg atcttgaaaa
     3061 caaaaacatt tatcttcctc gtctctatca aatcatggaa atcatcccgg agacttccga
     3121 catcaagact tttcgtctcg acttagaagg tgaaaattct tcccatctcc ctggacaatt
     3181 tgctgaactt tttgtaccag gggtaggaga agccccaatt tccatcactt cctctccaac
     3241 ccaaaaaggg atactggaat ttagcattaa aagaactggc ttagtaacct ctgccattca
     3301 tcgtcttaat cccaacgatc gtttgggtat ccgtggtcct tacgggaata cctttcccct
     3361 ttcagacctc accggacaaa acctactctt catagccggt ggtatcggat tggcaccgct
     3421 tcgttcctta ataaactacg ttttagatac cgaaaaacga aatcatttta aaaaagtaac
     3481 cattctctat ggagcacgtt caccacagga tctggttttt aaatgggaac tcaaaaaatg
     3541 gcaaaatcgg gaagacatta cctttttact caccgttgac cgaggagatg accaatggaa
     3601 gggtacagtt ggcttggttc ccaatgtact taaggaaaac gtccaggtga atccattaga
     3661 atggaagtcc atcatctgtg gccctccgat tatgattaaa tttaccatca aggcgttatt
     3721 ggaaatgggt ttccaacctc ctgatattat taccacactg gaaatgagaa tgaaatgcgg
     3781 attgggaaaa tgtggtcgat gcaatatcgg accctattat gtttgtaaag atggaccggt
     3841 ttttacttac cagcagctcc agaatatgcc ggaagagtac tgacggaggc aattacatga
     3901 acttccaaga aagtattcaa gctctctact cattaattaa ttatgaaaaa actgattttt
     3961 cttacaccga cctcaagctc gatcggatga gagaattcat gactctcctt gaacaccccg
     4021 atacccattc accagtgata ttggttgctg gtaccaaagg aaagggaagc acctgctatt
     4081 atttggaacg gatattagct tcccaacaga agaaatgcgg cttgtatatt aagccacacc
     4141 ttctcacttt tcgggaacgc ctgcggttta atggagaaat tattcaacct gaagaacttg
     4201 ctcagttagt aaccgatatt ttcccagtta tcaaccacat ggaaaaatat tctctttatg
     4261 gtaagcctac ctatttcgag gtttcggttg ccttggcttt ccaatatttt aaaaaaagaa
     4321 aagctgatta ttccatcatg gaggtagggc ttggaggccg tttggatgca accaatgttg
     4381 ccaatccaat tctcaccgtt atcactccga taagttatga ccataccgaa atattaggtg
     4441 aaaccattcc tcaaattgct caagaaaaag ccggtattct ccgtccctat attcctctta
     4501 ttttagggct tcaggaaaaa aaatccgcca agaccactat aatgcaagtt gttgaaaatc
     4561 tgcgggttcc agtatttcct cttgaagatt attacaccta ccgaattcgc agccgaagcg
     4621 caaatggatc ggtttttgaa ctgctagcaa aatctggccg agagggaatt ttttccttac
     4681 cgttgttagg tgatcaacag gttgaaaatt ttcttactgc tttgctcagc gcggaacatt
     4741 taggatattt accatccaat cagcagctcc aaaaccttct caatgaagca gtttggccgg
     4801 gacgaattca agttataggt tcatcaccct taaccatttt cgacgtggct cacaatcagg
     4861 catcttttgc cactttgtgt catactctga gagattattt ggggataaaa aaagccgtct
     4921 ttctcttagg ttttttaaat ggaaaggact ttccgggaat tgctgaagaa ctctttcaaa
     4981 tggaatcccg agtgatctta accacaccct ttaatccgaa agccgccctc ccagccgata
     5041 ttgttcattt ctttgcacct cttattcctc atcatgaagt gatcaatgac ccctaccagg
     5101 cttgggagaa agctcttcaa tacgcaactg aattccactt gcccttagtg gtagctggat
     5161 cattttactt agcaaaatta ttaagtgata aactcaacgt aaccctttcg aaagaggagg
     5221 tggagttatg attatagtcg ccgaacgaat caatgccacc cggaaagcca tccgggaagc
     5281 actggagaaa aaagatcagg aattttttat tcaagaagct caaaaacagg aaaaggctgg
     5341 tgcagctttt attgatgtca atgccggcac cgaagccacc aaggaagttg ctgatctgaa
     5401 atggttggtc gagtctattc aagatgcggt tgaaattccg ctatgcttag attctgccaa
     5461 tgatcaagct ttagaggaag ccctcaccgt ctataacaaa aaagaggtga tgattaattc
     5521 atttaccgct gaggaaaatc gaatcaaagc cttgcttcca attgtgaaaa aatacgatgc
     5581 tttggtggtc gggttggcga tgggggaggg aggaatcccg caatcggttg aagagcgaat
     5641 gaaattagta gagactttag tcaaagctgt tgatgaatat tctattcctc gagagcatct
     5701 ttttattgac ccgctggtag tgcccatcgg taccgatcaa aaccaaggga aattgttttt
     5761 agaaaccctc caggcaattc cacaaaccta tcctggtgtg agaaccgtct gtggtctcag
     5821 taatatttcc tttggaatgc ccaatcgtcg actcattaat cggtcctttt tagcattgag
     5881 tataggtttt ggccttcacg ccgccattat tgatcctctg gatgacaagc tcatgggaat
     5941 tctttttgcc agtgaagcct tgatgggtcg ggatgaatac tgcatgaatt atctcactgc
     6001 ttttcgagct ggccgtttaa acgagtaaaa ctttttaaaa acatcctctc tcctgtctct
     6061 ttcggtcagg agagagaaaa aattaatgat attgagctga ttcacgtttg agtggtaaat
     6121 atttaatccc aatagacgag gggggcatta atactcattc aggccataaa tcattcctca
     6181 ttctttataa tcctctggca aggaagggaa aaaagggtat aatacctctg aaaagaggtg
     6241 ttttctattc aattaaccaa agccaaagaa tttcgtcgtt acattgaaga tcattatgaa
     6301 ttcggcgact tcgctttgat tcgaggtcga gaagaaacag ccgagatcgg ttttgtattc
     6361 gccgatgaag atgttaataa ttggccttct ctttataaaa aagccgaaaa tatttgtgat
     6421 cattttgata aaagactaca agaggaggga ctcaaaaccg tagcctactc tcgagtagga
     6481 aaagacctgg attttatcac tgtgtcaatt gtcatccgtc ttcacgcctt ccctgaggat
     6541 caaatccatc gaattgccga tgtcattatg aatatccttc gagaagtaaa tccctatcat
     6601 gaaaatgaaa attaaactat gaaggaggct atcatgaagt tcagagggaa aattgtcatt
     6661 accttgaaac caggggtttt tgatccacaa ggagtaacca ttaaaagcgc actccattcc
     6721 cttggatacc aagaaactga tgaggttaaa actgggaaat atttcgaaat caaccttgat
     6781 tcaagcaacc tggatactgc tcaaaaacgt ttggaagaga tgtgtgacca attgctggct
     6841 aacccggtaa ttgaaaattt tcacttcacc gtcgaaccct ctgaaaacta aggagcctcc
     6901 aatgaaattt ggtatagtcg ttttccctgg ttctaattgc gattatgatt gtcaacacgt
     6961 tctttccgtg gttctcagcc aagaaacaga aatgctatgg catgaaagcc atgaccttaa
     7021 ggagtgcgat tgtattgtcc ttcctggagg gtttagctat ggagattact tgcgtactgg
     7081 tgctgtcgcc cgtttttcac cggtcatgga atcaattcgg aattttatcg aagagggagg
     7141 attagtcgtc gggatctgta atggatttca aatcttagtc gaaagtcaac tccttccagg
     7201 tgccttgctt cccaaccaga agggacattt catatgtcgt catgttcacc tgcgagttga
     7261 aaataccgat acgcctttta cccggctctt ttacccggga gaagtcatct caattcctat
     7321 cgctcatcat cagggaaatt actttattcc tcattcttct ttatccaatc ttcaaaaaaa
     7381 tcgtcaaatc atttttcgtt attgtgatat tgacggtcaa atcaatcatg caaccaaccc
     7441 gaatggatcg attgactcaa ttgccgggat aatttcttct tcccaaaatg ttctgggaat
     7501 gatgccacat cccgaaaggt catcagaagc agtattgggt tctcaggacg gaaaaagaat
     7561 atttctctcg attatccaat ggtgggagga aaaacatcat gcaaaataac gaacaaatcc
     7621 aggtagcccg tgagttaggg ctccgcgacg aagaatacca aaggatactt gacatattgc
     7681 aaagaccacc aactccaacg gaactggcca tgttttccgt cgaatggtca gaacactgtg
     7741 gctacccgca ttctcgccgt tggtttgaac tcttcccacg agaaggaaaa tttccggttc
     7801 tggtcggtga agatgccgga ggtattgttt atcaaggaca ggcagtggta tttaagatgg
     7861 aaagtcataa tcatccctcc caggtcgagc cccgccaagg agcagctact ggtattggag
     7921 gaatcattcg tgatattttt gggagcggtg cacgccccat agcttgcctt gattctctcc
     7981 atttcggtcc tttagataac gctcgttcaa attttatttt taaaggtgta gtagacggaa
     8041 ttgcttttta tgggaattgc gttggtgttc caaccgtagc tggcgaacta tattttcatc
     8101 cttcttatca gggcaactgc ctagttaacg tcatgagtat tggaatagcc tcacaagatc
     8161 gattggcaaa atcctgggcc aaagggaagg gaaatcttat tatctatgct gggaaccgaa
     8221 ccggacggga tggtatagga gggtgtagta ttttagcttc tcaggaattc agcgaacaag
     8281 atgagaaacg ccccagcgta caaattggtg atccctttac tgaaaaatgc ctgattgaag
     8341 caactatgga agctcttgcc actggattct tagttggaat caaggatatg ggtgccgctg
     8401 gtttgacttg ctcttccagt gaaatggctg ctgccggtaa aagtggggtt aaaatctatc
     8461 tggatcgtat tccggttcgt gaagaagcca tggaaccctg ggaaatcatg atgtctgagt
     8521 ctcaagaacg catgcttctc tgtgtaaaaa gaggttacga ggattcagtg cttcaaattt
     8581 ttcataaatg gggtttagaa gcggtggtcg taggagaggt agtcgatgga gacaatatca
     8641 ccattgagtt caatgggaag atggttgctg atattcccgc tcaagaatta actaaaccac
     8701 cggtttatac tccccccgcc gaaaagccag cttatcttga aaggataaat cattatgact
     8761 ggaatgatct tcctctgccc aataattgga acgaaatatt actcaaatta atggcttctc
     8821 ccaacctctg ttctcgtcat tcggtttttg aacaatacga tcacatggtt caaattaata
     8881 cctcaatgct gcctggaact ggaacggtta tcctccgtgt taaaaatctt ccttggggga
     8941 ttgctgttac cactgattgt aatccagtat tttgctacct cgatccttat cggggctctc
     9001 aaattgcggt tgctgaagca gctcgtaact tggctgcctg tggagctcaa ccagccggta
     9061 taaccgattg tttgaatttt gggaatccgc agaagcccga tcgttattgg caatttattg
     9121 aggcaattaa gggaataagc gatgcctgca aattttttga ccttccggtt gtgagcggca
     9181 atgtttcttt ttacaatgaa agtccaactg gtcctatcca tcccactcca accatcggca
     9241 tgattggtat tatcgaagac gttcaaaaag cggtaaatgc agcctttaaa gattttgatg
     9301 acgtgatcgt tttattgggt gaaacccgca atgaaatagg cgggagtgaa tatttacgtg
     9361 tgattcacca gcaagaattt ggaccggctc cacaaattga tttatcggct gaaaaaaatc
     9421 ttcaagaatt tttaatcgat gcagttcgac aaggttttgt tcaatcatct tctgatctaa
     9481 gcgaaggcgg attggcttgt gctctcggcg aagggtgcat ctgtggtcag gagaagaaag
     9541 gatgcctgat tgacatcgga aatttaccca atatccgctt ggatgctctt ttatttggag
     9601 aaagctgtgg gcgagctcta attacctgta aaccggaaaa ttttgctaca ctggaaaaga
     9661 tggctcggat gaaaactatt cccttttttg ttattggcaa agttaccagg gaaagcctaa
     9721 acatttataa taatcagcaa tcactgattt cagtttctat agaggatttg tggaaagcct
     9781 ggtaccatac tttgtagttt ttataggagt ttgatttatc atacccacca atgggtttac
     9841 aatataaaca tccatactgg aaactggtac cagataagga tgaaaatact catccaggaa
     9901 tcgtttcccc ctttagcaaa ggaagttagg gggtttgaaa taattgggaa aaaacaaatc
     9961 cttctcaatc ttcttttatc taaaaagaaa ggaataaaag agattgaatt catgagattg
    10021 ccacgtcact tcgttcctcg caatgacgat tcagagttcc ttctccct
//
LOCUS       DAOSOL010000008         9999 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6739_length_9999_cov_4.273230, whole genome shotgun sequence.
ACCESSION   DAOSOL010000008 DAOSOL010000000
VERSION     DAOSOL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6739_length_9999_cov_4.273230"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="PK171_00470"
     CDS             <1..1063
                     /locus_tag="PK171_00470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOT04708.1"
                     /translation="EYCSKFQSNVTKGFNSMSWSEICGHKLQKQFFQRVFQNQSQSHS
                     YLFSGPEGIGKTTFAFEIARLFNCEHPSNDGSCGSCQNCRLINAGTHPDVILLSPREN
                     KILVEDVRDFIFRLGLQRVYGKFRIGIVESTELFSKEEIQNCLLKTVEEPPENSIIIL
                     LTSQVQILLPTLLSRCQRIHFQLLNQESIKKFIKKKYNLTDQKAGDIAEQSLGRLRNA
                     FDLAEGNQPLIEKSFFLWDWITGENEIFSLGSWFIENKDKIIEIFSQLEWYLRDIMIF
                     QFIGRDGERILSHSGFKKRIVDDSNRLSPRIISRFILEIEQLEKDLKSNLNYDTTLLH
                     FVLMVREELNSAPCCRNQV"
     gene            1035..1832
                     /gene="ricT"
                     /locus_tag="PK171_00475"
     CDS             1035..1832
                     /gene="ricT"
                     /locus_tag="PK171_00475"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOT04709.1"
                     /translation="MPRVVGIKFEHNLKMYFFETRNFDLHYGQKCVVETVLGLEMGEV
                     VKRPFICENIKNSLKPVIRPAREIDILQLKSNREKEKIAFEIANQKIQEHQLSMKLLR
                     AHYTLDRGRLTFYFGSEERIDFRNLVKDLAAIFRTRIELRQMGVRDEAGMIGGCGMCG
                     RELCCSTFLINFEPISIKMAKEQNLALNSAKISGVCGRLMCCLSFEYSQYKKLIYQLP
                     KKGSKILTPQGLAKILEIDIFKDMIRLELEDGKEICINEEEYNRFFL"
     gene            1880..2488
                     /locus_tag="PK171_00480"
     CDS             1880..2488
                     /locus_tag="PK171_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT04710.1"
                     /translation="MGEAQVQKATWYQGLTAEQEKIWFEEQIRQCEKDIIRFACHITG
                     NMEKAKDLIQEAFLRAYKYRYSYNDKYPFRNWLYAILLNIHRHNCKKEKLFKTIIPWK
                     NSDDEEQDAWDFIESEEEGPEAKTLKKQLVVDIEKAIQKLPLKMREVIVLCDMIGYSY
                     EEASEVVHCPLGTIRSRLHRARRQVQKELEIKYGEDLLTSWS"
     gene            2494..2946
                     /locus_tag="PK171_00485"
     CDS             2494..2946
                     /locus_tag="PK171_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT04711.1"
                     /translation="MECERIRKLLSAYLDGELSEEERKEVRKHLFVCSRCELEFMKIR
                     NMKGLIIQFGQSFEPKVDYGIHYEAFQSQINGRRLKEILLFSVLACIIILLFVFFLPI
                     YKNIIPGSNTDASIGEIHRNLTGDSYDVPKNSGRVVVNFLKQVTNDWE"
     gene            2943..3908
                     /locus_tag="PK171_00490"
     CDS             2943..3908
                     /locus_tag="PK171_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04712.1"
                     /translation="MKKTSLFIGFIILLLVSFFTINSSQARILSPEEGRLFLKRIIQN
                     STAHSYWGIWKLTDYTGESESIFYEVVYIPRFGFGWINLDNPHHYIIGDGTYRFIYDE
                     EKQVITAVYPGFDLPFLPLGEDNIDILVSNYLIDVEEDVVMIISRENNQVVKSFLLDE
                     SQALISQTYYSKNGQVVKRGEYVYRIDYPDDERIWKIFQIMDQVIQKKNNFPEVLFNR
                     KRMLIPRLLPPGYSLRRSYLVEGDQGKMYQMVFSDGINFFSLFQTLLSPHSKYTSRLR
                     KIVIKKDGELTTLIGEKMGRQIFLVGNLSHEILSEIFESIADEGG"
     gene            3913..5340
                     /locus_tag="PK171_00495"
     CDS             3913..5340
                     /locus_tag="PK171_00495"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOT04713.1"
                     /translation="MNRKIKFEFLLCFSVLILFLLTGWVQAVNSIIPEETNLVADIVE
                     KVGPSVVYVDTLSYKTYRNPLSPFFNDPFFRDFFDIFPQPEERRIPQKGLGSGFIFRS
                     DGYILTNEHVIQGAEQIKVTLKDGREFDGKVIGSDPLTDIAIIKVDATDLPALPLGDS
                     DRARVGEWMIAIGNPYGLSHTVTVGVLSAKGRPIYSGDSGREYENFLQTDAAINPGNS
                     GGPLLNIKGEVIGINTAILPYAQGVGFAIPINMAKSLLDPLIETGKVVRSWVGIYLQD
                     ITPDMIQQFGLNEPKGALIADVVPNSPASRAGIQRGDIILKVDDEEIVNAAVFHAAIQ
                     DKKPGIQLTLSVWRDNQAKEITVILEELVTEGGVESALSPDLQFGFEVGEITSELTRK
                     YNLRTKSGVVIIRIDSQEIIETGYLREGDVILQLNRQTIRDLQGWNAALTMVEPGDTI
                     ILLVNRRGRTFFVPVEVKSSSLLVP"
     gene            5337..6215
                     /gene="rsmI"
                     /locus_tag="PK171_00500"
     CDS             5337..6215
                     /gene="rsmI"
                     /locus_tag="PK171_00500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT04714.1"
                     /translation="MKKFSEWGNFFICPTPIGNLGDITYRTVSVLNQVDLIACEDTRV
                     SRKLLQRYSIQKPLFSYHAHNYQAAIQKILTHLKSGESVALITDAGMPGIQDPGMEIV
                     NQLVQNQIDFEVLPGASAVLPAVVYSGFSLNGYLFLGFLPKSGGERKKQLEQSLFSPQ
                     AVVLYESPKRILTTLQEIQSLVGEERKAVLCRELTKIHQEIIRGTLKEIIEILNQRKT
                     IKGEMVLVLEGIVVDEKRILPLIQNCIKQLLFTGHSEKDTVELTSVIFGLKKNRLKFE
                     VKHQIDLLGEGERKRI"
     gene            6322..7221
                     /gene="pstC"
                     /locus_tag="PK171_00505"
     CDS             6322..7221
                     /gene="pstC"
                     /locus_tag="PK171_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOT04715.1"
                     /translation="MNSVQEVFRIKAHRESAEIILFLVALSSLLFLIGIIVVLFVEGL
                     PTFREVSLSQFLFGRRWYPTSEPPRLGILPLILGSLWVTIGALIFAIPLGLFSAIFLA
                     ELCPKKLREILKPVIEMLAGIPSVVYGFFGMVILSPLLKDLFSLPTGLTAFTASIILG
                     IMAIPSIVSTAEDAISSVPKSYREASFSIGATHWETIRKVVIPASYSGLGAAVILGAG
                     RIIGETMTVLMIAGGAAIIPTSFFQPVRTMTATIAAEMGEAPVGSLHYHSLFAIAIVL
                     FLITFLLNILVDRLAQKYHTRVR"
     gene            7223..8089
                     /gene="pstA"
                     /locus_tag="PK171_00510"
     CDS             7223..8089
                     /gene="pstA"
                     /locus_tag="PK171_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOT04716.1"
                     /translation="MDIRLKTKYLSQKIWFFIFFLSIATICFALGFIIYFLVVRGYKV
                     INWEFLTALPSRGMTEGGILPAIVGTLYLIIGSIVVSLPLGVFSAVYLEEYAKPSPTV
                     TLLRLAIHCLAGVPSVVFGLFGLGIFVKLFGFGVSLLSGSLTLGIMALPIVITSVEEA
                     LKTVPLSFREASLALGATKWVTIRRVVLPAALPGILTGLILSVGRVAGETAPILFTAA
                     AFYARGLPRSIFDRVLALPYHIYGLMTEGTRPEKQVPIAYGTALVLLIMVLLVNFLAI
                     YLRRRSRSARKW"
     gene            8094..8858
                     /gene="pstB"
                     /locus_tag="PK171_00515"
     CDS             8094..8858
                     /gene="pstB"
                     /locus_tag="PK171_00515"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOT04717.1"
                     /translation="MDKDESKLKVLNFNANFGEKQILYDISLEIPDNRVTAIIGPSGC
                     GKSTLLRSVNRMNDFIENFLIRGEIIFENQNIYGEEVDPVELRQRIGMVFQRPNPFPK
                     SIFENIAYGLRVQGWKDRQAIAEQVEKSLLAAALWDEVKDRLNSLAFDLSGGQQQRLC
                     IARAIAVEPEILLMDEPASALDPIATARIEELIKDLKEKYTIVIVTHNMQEAARTSDY
                     TAFLLLGRLIEYGLTDQIFTNPQKKETEEYLTGRFG"
     gene            8875..>9999
                     /locus_tag="PK171_00520"
     CDS             8875..>9999
                     /locus_tag="PK171_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT04718.1"
                     /translation="MKFRFFSWLLGMCLVFSLGFFSVYAQEAPTIAILPLSMRDEVDG
                     YLGYYLRDLIKDALDDMEQVEVIDNIMIDEILRESKIPRDVVLVQSMAQVLGKRIGGD
                     YLLSGSYRIRQLGDKERLVVSVRLFDLNQEVMIDLKSNVFDINNPDEFRQLIVPEVLK
                     SLNIDFALSVEPFRYETQLIFPLYRAVEKMDEALRTYGSAQFPDKPLWKEAFAEAQET
                     IDAVPEYLESYYYLAYMYQKTGWLAKEVETWMEYVKLLEGSGKGKNSIQRASRAYLRL
                     ANSYLSQKKYDMAEQSINQALQINPDIAEAYFLLGKIAYDQGKSAQAQEYWNKAYFLD
                     PSLKEAQYFAGEAGKAAVYGKDAYESYRTGYTFYANGDLKT"
BASE COUNT         2984 a         1730 c         2117 g         3168 t
ORIGIN      
        1 ggagtattgt tcgaagttcc aatcgaacgt tacgaaaggt tttaattcga tgagttggtc
       61 agagatctgt gggcataaat tacaaaagca gttttttcaa cgagtttttc aaaaccaatc
      121 ccaaagtcat tcttatcttt tttctggacc agaaggaatt ggaaaaacca ctttcgcttt
      181 tgaaattgcc cggttgttta attgtgagca cccatctaat gatggttcct gtggctcttg
      241 ccaaaattgc cgtctgatca acgctggaac ccatcctgat gtcatattgc tttctcctcg
      301 ggaaaataaa attttagtgg aagatgtacg agattttatt tttcgtttag gactacaaag
      361 agtctatggg aaattccgga ttggaattgt tgaatcaact gaactattta gtaaagaaga
      421 aatacaaaat tgtcttttaa aaacagttga agaacctcca gagaatagca taataattct
      481 ccttacttcc caggttcaga ttttattgcc aactctcctt tctcgatgtc aaaggatcca
      541 ttttcaatta ttaaatcaag aaagtataaa aaagttcatc aagaaaaaat ataatcttac
      601 cgaccaaaaa gctggagata ttgccgagca atcactgggt cgtcttcgga atgcatttga
      661 cttagcagaa gggaaccaac ctctcattga gaaatcgttt tttttgtggg actggattac
      721 gggtgaaaat gaaatttttt cacttggttc ctggtttatt gaaaataaag ataaaataat
      781 tgaaatcttt tcacaactgg aatggtactt acgagatatc atgatttttc aattcatagg
      841 aagggatggc gagcgaatcc tcagccattc tggttttaaa aagcggattg ttgacgattc
      901 aaatcggctg tcgccaagaa ttatttctcg ttttatcctt gaaattgaac agttagagaa
      961 ggatctgaag agcaatctca attatgatac cactttgttg cattttgttt taatggtaag
     1021 ggaggaattg aatagtgccc cgtgttgtcg gaatcaagtt tgaacataat ttaaaaatgt
     1081 atttttttga aacaagaaat tttgatttac attatggcca gaagtgtgta gttgagacgg
     1141 ttttaggctt ggaaatgggt gaagtagtaa aaagaccctt tatttgtgaa aatattaaaa
     1201 atagtctaaa gccggttatt cgacctgctc gggaaattga tattttgcag ctgaaaagta
     1261 accgggaaaa ggaaaaaatt gcctttgaaa tagcgaatca aaaaatccaa gaacaccagc
     1321 tttccatgaa attgctaaga gctcattata ccctggatag gggaagatta actttttatt
     1381 ttggatctga ggaaagaatt gatttccgaa atttagttaa agatttagcg gctattttcc
     1441 gaacccggat tgaacttcgc cagatggggg ttcgagatga agcagggatg attggagggt
     1501 gcggaatgtg cggtcgtgag ttgtgttgta gcacttttct tatcaatttt gaaccaatat
     1561 cgattaaaat ggctaaagag caaaacttgg ccctcaattc ggcaaaaatt tctggagtct
     1621 gtggccgatt aatgtgttgc ttgtcttttg agtattctca atataaaaaa ttgatttacc
     1681 aacttcccaa aaaggggagt aaaattttaa cacctcaggg attagctaaa attcttgaaa
     1741 tagatatttt taaggatatg attcgcctgg aactcgaaga cgggaaagag atatgtatta
     1801 atgaagaaga atacaaccgt ttcttccttt agttcttttt ctgataggaa attggaagga
     1861 taaagggtgg tggaaaaccg tgggagaagc gcaggttcaa aaagcaacct ggtatcaagg
     1921 cttaacagcg gaacaagaaa aaatctggtt tgaagaacaa atacgacagt gtgaaaaaga
     1981 cattatccga tttgcctgtc atattactgg taatatggaa aaagccaaag atttaataca
     2041 agaagctttt ctaagagcct ataagtatcg ctatagttat aacgacaaat atccttttcg
     2101 aaattggctt tatgccattc ttttaaatat tcaccgtcat aactgtaaaa aagaaaagct
     2161 ttttaagacc attattcctt ggaaaaattc cgatgatgaa gagcaggatg cttgggattt
     2221 tattgaaagc gaggaagaag gtcccgaagc gaaaacttta aaaaaacagc tggttgttga
     2281 tattgaaaaa gccattcaaa agcttcccct gaaaatgaga gaagtaatcg ttttgtgtga
     2341 catgattggg tattcctatg aagaagctag tgaagttgtt cattgtccat tggggacaat
     2401 tcggtcgaga ctccatcgag cccgtcgtca agttcaaaag gaactggaaa ttaagtatgg
     2461 agaagatttg ctcacttctt ggagttgaga attatggaat gtgaacggat tagaaaatta
     2521 ctttcagctt atcttgatgg tgagctgtct gaagaagaac gtaaagaagt gcgaaagcac
     2581 ctttttgtct gttctcgatg tgaattggaa ttcatgaaaa ttcgaaatat gaagggattg
     2641 attatccaat ttgggcaatc ttttgaacca aaagttgact atggcattca ttatgaagct
     2701 ttccaatctc aaataaacgg acgacgattg aaagagattc tccttttttc agttttggcc
     2761 tgtattatta tccttctttt tgtttttttt ctccctattt ataaaaacat tataccagga
     2821 tcgaatactg atgcatcgat tggggagata catcgcaatc ttaccggcga tagctatgac
     2881 gtgcccaaaa actcagggag agtcgtggtt aattttctaa aacaagttac aaatgactgg
     2941 gaatgaaaaa aacatctcta tttattgggt ttattattct tcttctggtt tcttttttta
     3001 ctatcaattc ctctcaagct cgcattttga gtcctgaaga aggtcgactt tttttaaaac
     3061 gtattatcca gaatagtact gcccatagtt attggggaat ttggaaactt actgattata
     3121 caggtgaaag tgaaagcatt ttctatgagg tggtgtatat tccccgcttt ggttttggct
     3181 ggataaatct tgataatccc catcattata ttattggtga tgggacttat cgttttattt
     3241 acgatgaaga gaagcaagtg attactgctg tatatcccgg ctttgatctt cctttccttc
     3301 ctctgggaga agataatatt gatatattag tttcaaatta tttgattgat gttgaagaag
     3361 atgtcgtgat gatcatttct cgtgaaaata atcaggtggt gaagtctttt cttttggacg
     3421 aaagccaagc gctcatcagc caaacttatt attctaaaaa cggacaggtg gtaaaacggg
     3481 gggaatatgt ttatcgcatc gactaccccg atgatgaacg aatttggaaa attttccaaa
     3541 taatggatca agtcattcag aagaaaaata atttccctga ggttcttttt aatcggaaac
     3601 gaatgttgat tcctcgtttg ttacctcccg gttattcttt gcgtcgatct tatcttgttg
     3661 aaggtgatca gggaaaaatg taccaaatgg tatttagcga tgggataaat tttttctctt
     3721 tgtttcaaac tctcttatct ccccattcaa aatataccag ccgacttcga aaaatcgtta
     3781 ttaaaaaaga tggagagctg actactctta tcggagaaaa aatggggaga caaatttttt
     3841 tggttggaaa cttgagtcat gaaattttga gtgagatatt tgaatcaatt gctgatgaag
     3901 gaggttgatg atatgaatag aaaaataaag tttgagtttt tattatgctt tagcgtttta
     3961 attctctttt tattaaccgg ttgggttcag gctgtcaatt caatcatacc tgaagaaacc
     4021 aatctggtag ctgatattgt tgaaaaagtc ggaccgtcag tggtgtatgt tgatacactt
     4081 tcttataaga cctatcggaa tcccctttca ccttttttta atgacccctt ttttcgagat
     4141 ttttttgata ttttccctca accagaagaa aggagaatcc ctcaaaaagg attaggctcg
     4201 ggatttatct tccgatctga tgggtatatt ctcaccaatg agcatgttat tcaaggtgct
     4261 gaacaaatta aggttactct caaggacggc cgggagtttg atggaaaggt aataggttcc
     4321 gatccattaa ccgatattgc gatcataaaa gtggatgcaa ccgatcttcc tgcccttcct
     4381 ttgggtgatt cagatcgagc ccgagtggga gaatggatga ttgccatcgg aaatccttat
     4441 ggtttgtcgc atacggtaac ggttggcgtt ttgagtgcaa aagggagacc aatatattcc
     4501 ggagactcag gaagagaata tgagaatttt ttacaaaccg atgctgccat taaccctggt
     4561 aatagcggag gaccactatt gaatattaaa ggcgaagtta ttgggattaa tactgctatc
     4621 cttccttatg cccaaggagt tggattcgcc atccccatca atatggcaaa aagtttatta
     4681 gatccattaa ttgaaactgg caaagttgtt cgttcctggg tagggattta tctacaggat
     4741 atcactccgg atatgattca acaatttggg ctcaatgaac caaaaggcgc tttaattgcc
     4801 gatgttgttc caaatagtcc agcttcgaga gccggtattc aacgcgggga tataatatta
     4861 aaagttgatg atgaagaaat tgtcaatgct gcggtatttc atgccgcaat tcaagataaa
     4921 aaacctggaa tacaattgac attgtcggtt tggagagaca accaagccaa agaaattacc
     4981 gtaatcttag aagagttggt taccgaagga ggagttgaat cagccctatc tccagacctt
     5041 caatttggtt ttgaagttgg agaaatcacc tcagaattaa ccagaaaata taatttaaga
     5101 acgaagtctg gagtagtgat cattcgaatc gactcgcaag aaataatcga aaccggttat
     5161 ttacgagagg gagatgtcat tcttcagctc aatcgtcaga ccatcagaga ccttcagggc
     5221 tggaatgccg ctttgacaat ggttgagccc ggcgatacta taattctatt ggtaaatcga
     5281 agagggcgta ctttttttgt accagtggaa gtgaaaagtt ctagcttatt agtcccatga
     5341 aaaaattttc ggagtggggc aattttttta tttgccccac tcctattgga aacttgggag
     5401 acattaccta ccgtacagta tcggtactca atcaggtgga tctgattgct tgtgaggata
     5461 ccagggtatc gagaaagctt ttgcaacgct attcaattca aaaaccttta ttttcctatc
     5521 atgcccataa ttaccaagct gcaattcaaa agatccttac ccaccttaaa agcggggaaa
     5581 gcgtcgcttt aattactgat gctggaatgc caggaattca agatccagga atggaaattg
     5641 taaatcaact ggttcaaaat caaattgact ttgaagtgtt gccaggtgca tcagcagtct
     5701 taccggctgt ggtttactca ggattttctt taaacggtta tcttttttta ggttttcttc
     5761 ccaaatcagg aggagaaagg aaaaaacaac tggagcaatc acttttttct ccccaagccg
     5821 ttgttttata tgagtcaccc aagagaatcc tgaccaccct tcaagaaatt caaagcctgg
     5881 ttggagaaga aagaaaggca gttctctgtc gtgaattaac aaaaattcat caagagataa
     5941 taagaggtac tttaaaagag ataattgaaa tattaaatca gaggaaaacc attaaaggtg
     6001 aaatggtatt ggtgctggag ggtatagttg ttgacgaaaa aagaatttta cctttgatcc
     6061 agaattgtat caaacaactt ttatttactg gtcattctga aaaagataca gtagaattga
     6121 ccagtgttat tttcggactt aaaaaaaata gattaaaatt cgaggtcaaa catcaaatag
     6181 atcttttggg tgagggggaa agaaaaagaa tttagttaga ttaaacctca aaatattgat
     6241 taaaaataca tatcttgaag tgaactttaa tacctctttt taaacattac agaattgatt
     6301 ttattcttaa aaattggttt aatgaattcg gtacaggagg tgtttcgtat taaagcgcac
     6361 cgcgaatctg ccgaaattat tttatttttg gtcgcccttt cttccctttt atttttaatt
     6421 ggaattattg tcgtactctt tgttgaagga ctgcctacct ttcgtgaagt ttcgctctca
     6481 cagtttcttt ttggaagacg atggtaccca acatcagaac cccctcgttt aggaattctc
     6541 cctctaatcc ttggatccct ttgggtgact attggtgcgt taatttttgc aattccttta
     6601 ggcctttttt cagcaatatt tcttgccgag ctttgtccaa aaaagctgag agaaatctta
     6661 aaaccggtta ttgaaatgtt agccggtatt ccttcggtgg tttatggttt ttttggtatg
     6721 gtcattctca gcccactttt aaaagacctt ttctctcttc ccaccggtct gactgctttt
     6781 actgcctcga tcatattggg aataatggcc attccttcaa tagtgagtac agctgaagat
     6841 gcaattagtt cagttcccaa gagttaccgt gaagcttctt tttcgattgg agcgactcat
     6901 tgggaaacca taagaaaagt tgttatacct gcttcttatt caggcttagg ggcagcggtg
     6961 attcttggtg cagggagaat cattggtgaa accatgacag tgttgatgat tgccggtgga
     7021 gcagcgataa ttcccaccag cttttttcag ccggttcgaa ccatgacggc aaccatagca
     7081 gctgaaatgg gagaggcacc ggtaggtagt cttcactatc attcgttatt tgctattgcg
     7141 attgttttat ttctcatcac ttttctttta aatattttag tcgatcgatt agctcaaaaa
     7201 taccataccc gggtgagata aattggatat tcgcttaaaa acgaagtatt tatcacaaaa
     7261 aatctggttt tttatctttt ttctctccat agccactatt tgttttgccc tgggttttat
     7321 catttatttt ttagttgttc gagggtataa agtaatcaat tgggaatttc tcaccgcttt
     7381 accttcccga gggatgaccg aaggaggaat tcttccagca atcgttggga cgctttatct
     7441 cataatcggc agtattgttg tttctcttcc tcttggagtt ttttcagcgg tgtatttaga
     7501 agaatatgcc aaaccatcgc caacggttac gctgctccga ttagcaattc attgtttagc
     7561 tggtgttcct tcggtggttt ttggcttatt tggtttggga atatttgtta aattatttgg
     7621 atttggagtc tctcttctct ctggatcgct taccttgggg attatggctc taccgatagt
     7681 cattacttcg gtagaagaag cgttaaaaac tgtaccactt tcctttcgag aagcttctct
     7741 ggcacttggt gcaaccaaat gggtgacaat tcgacgggtt gtacttccag ctgcgcttcc
     7801 tggtatatta accggattaa ttctgtcggt tggtagggtc gctggtgaaa ccgcccccat
     7861 tttattcaca gccgcagctt tttatgcccg agggcttcct cgctctattt ttgaccgggt
     7921 tttagcgctt ccctatcata tatacggttt aatgaccgaa ggtactcgcc ccgaaaaaca
     7981 agtaccaatt gcctatggta ctgctttggt ccttctaatt atggttctct tagttaactt
     8041 tttagccata tatctcaggc ggcgatcaag atcagctcga aagtggtgaa agtatggata
     8101 aggatgaaag caaattaaaa gtattaaatt ttaatgccaa ttttggagag aagcaaatat
     8161 tatacgatat tagccttgaa ataccagata atcgggttac agcaataatt ggtccatcgg
     8221 ggtgtggtaa gtcaacttta ttacgcagtg ttaatcgcat gaacgacttt attgaaaatt
     8281 ttttaattcg tggtgaaatc atttttgaga atcagaatat atatggagaa gaggttgatc
     8341 cagttgagct ccgacagaga ataggaatgg tttttcaaag accaaatcct tttccaaaaa
     8401 gtatttttga aaatattgct tatggacttc gagttcaagg ttggaaggat cgccaggcaa
     8461 ttgctgaaca ggttgaaaaa agtttactgg cagctgcgct atgggatgaa gtgaaagatc
     8521 gtttaaattc attggcattt gatctttcag gaggtcaaca gcaacggctt tgtattgctc
     8581 gagctattgc ggttgagcca gaaatacttc tcatggacga accagcttca gccttggatc
     8641 caatcgctac agctcgaatt gaagagctca tcaaagatct caaggagaaa tataccattg
     8701 tgatcgttac tcataatatg caggaagctg ctcgtacctc ggattatacc gcttttctct
     8761 tattgggacg attgattgaa tatgggttga ctgatcaaat ttttaccaac ccccaaaaga
     8821 aagaaaccga agaatatctc accggtcgat tcggttgata atgaggtgaa tcatatgaag
     8881 ttccgattct tttcctggtt gttgggaatg tgcctggttt tttccctggg atttttttct
     8941 gtatacgccc aagaggcgcc tactatcgct attcttccac tttcgatgag ggatgaagtc
     9001 gatggatatt taggttatta tcttcgagac ctcatcaaag atgctcttga tgatatggaa
     9061 caggttgaag tcattgataa catcatgatt gatgagatct tacgtgaatc aaaaattcct
     9121 cgagatgtag ttctcgttca gtcgatggct caagttttgg gaaaaagaat cggaggagat
     9181 tatttactga gtgggtccta tcggattcgt caactggggg ataaagagcg tttagttgtg
     9241 agtgtgcgcc tttttgattt gaaccaagaa gtcatgattg atttaaaaag taatgttttt
     9301 gatatcaaca atcccgatga gtttcgacag ctgattgttc ccgaggttct aaaatcactt
     9361 aatattgatt ttgctttatc cgttgaaccg tttcgttatg aaacccaatt gatctttcct
     9421 ctctatcgtg ctgttgagaa aatggatgaa gcgcttcgga cctatggaag cgctcaattt
     9481 cccgataaac cactctggaa agaggctttt gccgaagctc aagaaaccat tgatgctgtc
     9541 ccagaatatt tagagagcta ctattatctc gcctatatgt accaaaaaac tggatggttg
     9601 gcaaaagaag tcgaaacttg gatggagtat gtcaagctcc ttgaaggttc gggaaaggga
     9661 aaaaactcta tacaaagagc gtctcgggcc tatttaagat tagcgaattc ttacctatct
     9721 caaaagaaat atgatatggc tgagcagagc attaatcagg ctttacagat aaacccggat
     9781 atcgcagaag cctattttct tttaggcaaa attgcttatg accaaggaaa gtcagcccaa
     9841 gcacaggaat attggaataa agcctatttc ttggatccat ctctcaagga agcccaatat
     9901 tttgctggtg aagctgggaa agcggcagtc tatgggaagg atgcttatga atcctaccga
     9961 accggttata ccttttatgc caatggtgat ctaaaaaca
//
LOCUS       DAOSOL010000009         9990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6745_length_9990_cov_4.297433, whole genome shotgun sequence.
ACCESSION   DAOSOL010000009 DAOSOL010000000
VERSION     DAOSOL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6745_length_9990_cov_4.297433"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..961
                     /locus_tag="PK171_00525"
     CDS             65..961
                     /locus_tag="PK171_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOT04719.1"
                     /translation="MTEEKKNKRFLTRGYRRDGTWLKDANPMHRLEPFIFKTRNGSCI
                     YFREKLDITHTLEYLQERNLNRSPEKKFTLFHILMTAMVRTAILKPGINRFIAGKRIY
                     QRNNMQISYLVKQEKTEAARMLAVKETFCSSDTLDQVAEKVLLSIERARHENAADSES
                     LVKTFSKLPGWMISFVVAFLRWLDNFGLVPESITSSDPFYASAFVANIGSLGVNAPYH
                     HLYEWGTVSLFVAMGKYAEELSLDQDGKLVKKTLVEITFTVDERIADGFYLARALQTF
                     KQLMEHPEELEKPVEEGMHNDT"
     gene            951..2603
                     /locus_tag="PK171_00530"
     CDS             951..2603
                     /locus_tag="PK171_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HOT04720.1"
                     /translation="MIPNSDQSMAERLFTTAEMYPDMPALDYFGKQITYQHLQEKIQR
                     LAMIWKNLGIEPGHRVIVCLPNIPEVVMTIYSLNMIGAVVCIVHPLSTAPEMSFYVQD
                     TQSQWLITLDILYGNFRKAVEESDIKKTILTTVSGELPPIPRYLYYLKQGRKSPNYHG
                     DRLIDWKTLIRNWLSPLKVFHHFSTDQPAVILFSGGTTGSPKGVLLSNQNMNALADQV
                     LTRVNPQPGKDSTLCILPLFHGFGLGICLHSVLLGGGRCILIPRFSKKEFIDAIAKCK
                     PTYIAGVPTHYEALINSEKLQKAQFSYLKGAFCGGDSVPLSLIKRFNDFMQSHGGIVS
                     LREGYGLTECVTACSIMPEDIYKERSVGLPLPGNRFKIVRPQGIEEVPVGEVGEICIN
                     GPTVMLGYNNRPEETDRVLQKHKDGLIWLSTGDLGTLDQDGFLYFTGRMKRIIKCSGY
                     AVYPSQVESVINSHPLVVESCVIGIPDEYTMSRVKAYVVIRGNVHSREKLAEEIKQSV
                     AEQLIKWSVPTEITFLDSLPVTRMGKADYRALEAMERGEAII"
     gene            2720..3679
                     /locus_tag="PK171_00535"
     CDS             2720..3679
                     /locus_tag="PK171_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT04721.1"
                     /translation="MKCVEKYDFCMDTVVYQKVYGKNAEEAIDEATRKIRYLEGKLSF
                     FQEESDIFQINIHAGKNWVKVSQDTIDVLQKAVEVSKSSNGAFDVTVGVLTDYWRQLK
                     KNQKKPDKNQIQDLISLINYRDILINSEERLVKLIRFNQKIDLGGIGKGYAGEKVIDV
                     YRDYSIQSGFINLGGNVVTIGKTHWKRSWIIGIRNPFSADGGIFGNLKVEEQAVVTSG
                     GYERYYEIDGLKYHHILDPRTGFPARLEWQSVTMVAESSTLADALSTAIFIMNRQELI
                     RFLKNFPSLGVILVAYTGEIMISKNLKEIFQKTNPEMSIIYID"
     gene            3715..4095
                     /locus_tag="PK171_00540"
     CDS             3715..4095
                     /locus_tag="PK171_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT04722.1"
                     /translation="MGTLAIVLIIVAVILVIIFIFASAGLKKVGKLNIGKIDLSKLAD
                     GVYPGEFKGYRWSNQVEVTVKNHQIAGIKIIKDIVFGSGDISKKIFDQIIQKQSLQID
                     TVSSATVTNKAYLKAIENALLKGK"
     gene            4555..5808
                     /locus_tag="PK171_00545"
     CDS             4555..5808
                     /locus_tag="PK171_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT04723.1"
                     /translation="MKHKGIFFITLILLVFSFSVMVSAKVSLSALFMKQAGYQESDMI
                     AMTDEFLKQNPDVEVNLNFVSYEELHDKIAVAAASESGVYDVIISDCVWPAEFAEAGW
                     IIDVTDRLKDEDRADIFPTVLNSVTYKDRIYGMPWLNDWQYFFYNKKMLNEVGASVPT
                     LWPEVIETSQKMKANGIIEYPIIDSWGQGEVVVVQYLTYLNAMGGKAFDENGLPILNQ
                     GKGLDALSYMVDNLKAGIYNPASIESFNEEVRRIYSSGQAAFGLNWIYMYNLSNDPQE
                     SQIAGDSEIALIPGFPDGNKSASCNGGMGLSIVNTCKNPDVAWDYISFLCSRDIQKKY
                     SANALPIWISLYDDPELIALQPKAIPIAKEQIQYVFDRPQLPWYSQFSQLLGEELQSA
                     ITGAKIPQQALDDAVARMKEIQERY"
     gene            5817..6773
                     /locus_tag="PK171_00550"
     CDS             5817..6773
                     /locus_tag="PK171_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT04724.1"
                     /translation="MQPKQQGLILLLFRLQIELTMKSNQYNRREVRFAWLLIIPAFIF
                     IGIIIFYPMVMAFYTSLHQVDLTRKAQGMPFIGFKNYIDILKSSGFWASINRTAYFTV
                     VSIVIEMVIGFLIALLLNQKFWARGILRSLLLVPWALPITVDAIMWKWIFNANYGAFN
                     ALLKQIGLIDSYRGWLTRPWSAMHCVIVADVWKVTPLVALLLLAGLQTIPREMYEAAT
                     VDGASWWRSLFSITLPLLKPTLTVVLVMRTLDAFRVFDIVYIMTSGGPANATKVISFM
                     TYQEAFKFLNFGRGSALAYLITLIVALLAYIYTINIGKHIEY"
     gene            6826..7677
                     /locus_tag="PK171_00555"
     CDS             6826..7677
                     /locus_tag="PK171_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT04725.1"
                     /translation="MIVKKYKIYRTIGITVGSIILVAGLLAPLVWLLISSVADLKDLL
                     KIPLQWIPENISFERYSKILFSQGSESEFRKTMMNSFVVASAVTIICVSIGSLSAYAF
                     SRLRFPGKDKILFVLLFSYMLPPVAIIIPIYQIFSRYNLLDTKTALILVYSAIITPFV
                     IWIMRTYFDSIPRDLEDAAKIDGCTYLGMLFRIMIPLSAPGIVATLLLAFLMSWEEFF
                     MALIMTSSVVSKTIPISIAEFSGRHSIDFGMMATGGILAAIPPVIIALIFQKYIVGGL
                     LSGAVKG"
     gene            complement(8264..8584)
                     /locus_tag="PK171_00560"
     CDS             complement(8264..8584)
                     /locus_tag="PK171_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT04726.1"
                     /translation="MDVIAIFAVVERGKADHLVEKAKKAGAKGATILYGRGTTAENDI
                     KHFLSFHVESSKEVIIILAEKEHVEAIQKALIEAGRLDEPGTGILFSVPVLHLTGLKH
                     RGLP"
     gene            complement(8599..9300)
                     /locus_tag="PK171_00565"
     CDS             complement(8599..9300)
                     /locus_tag="PK171_00565"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HOT04727.1"
                     /translation="MRITYLFHDIFDIVKSIIPVVSVLVFFQVVVLRQPLAHPKTFFS
                     GIVLAIVGLFLFLKGVTMSLIPLGESVGQNIVTLDNKLLISIFAFVVGYGATLVEPAL
                     RTLVLEVEEVSVGAIPEKVLLHTIAIGFGLGVTLGIIKIVSNIPTVKIVIPLLLFALY
                     LAYLAPEKMVGIAFDCASATTGPVNIPINVALALGLSRVVEGSDPLLNGFGIVGLSSL
                     GTIIAVLMLGIITKA"
BASE COUNT         3101 a         1742 c         2063 g         3084 t
ORIGIN      
        1 atccttatgc gattagaagt atcttttggt atagcaaaga atttaaggaa aggttgcgtt
       61 atgagtgact gaagaaaaga aaaataaacg ctttttaacc cggggatacc gtcgtgacgg
      121 cacctggttg aaagacgcta atccgatgca tcgtttggaa ccattcattt ttaaaacccg
      181 aaatggatca tgcatctact ttcgagaaaa gttagacatt actcatactt tggaatacct
      241 ccaagaaaga aatttaaatc ggtcaccaga aaaaaagttt actctcttcc atattctcat
      301 gacggctatg gttcgtaccg ctatcctaaa acccgggatc aaccgtttta tagctgggaa
      361 gcgaatctat cagcgaaata atatgcagat ttcctacctg gttaaacagg agaaaaccga
      421 agcggccaga atgttagcag ttaaagaaac cttttgttcc tcagacaccc ttgaccaggt
      481 tgctgaaaaa gtattgctat ccatagaacg agctcgtcat gaaaatgctg ccgatagtga
      541 aagtttggtt aaaacctttt cgaagctgcc cggttggatg atatcatttg tggtggcttt
      601 tctccgctgg ttggataatt ttggtctggt gccggagtcg ataaccagtt cggatccttt
      661 ttatgccagt gcttttgttg caaacatcgg tagtttagga gtgaatgcac cctatcacca
      721 cctctatgaa tggggaacag tttcactttt tgttgctatg ggaaagtatg ccgaagaatt
      781 gagtttagac caagacggta aactggttaa aaaaaccttg gttgaaatca cctttactgt
      841 agatgaacga attgccgatg gattttattt agctcgtgct ttacaaacct tcaagcagct
      901 catggaacat ccagaagaat tagagaaacc cgtagaggaa ggaatgcata atgataccta
      961 attctgacca gtccatggcc gaacgtttgt tcacgaccgc agaaatgtat cctgatatgc
     1021 cggctttgga ctattttggg aagcagataa cctatcaaca tttacaagaa aaaattcaac
     1081 gattagctat gatatggaag aacttaggga ttgagcccgg tcatcgagtg attgtatgtt
     1141 tgcctaatat ccctgaagtg gttatgacaa tttactcgct caacatgatt ggagcggtag
     1201 tctgtattgt ccatccctta tccactgctc ctgaaatgtc tttttatgtt caagacaccc
     1261 aaagtcagtg gttgatcact ttggatatcc tgtatggtaa ctttcgcaaa gctgtcgaag
     1321 agagtgatat caaaaagacc atattaacta cggtttcggg agaactccct ccaattcctc
     1381 gatatcttta ttatttgaaa caaggacgta aaagtcccaa ttatcatgga gatcgtttaa
     1441 ttgattggaa gaccctgatc cgaaactggc tctcacctct aaaagtattc catcattttt
     1501 ctactgacca accagctgtt attcttttta gtggaggaac cactggttcg ccaaaaggag
     1561 tccttctttc caatcagaac atgaatgccc tggctgacca ggtgttgact cgagtgaatc
     1621 ctcaaccagg taaagattca acactctgta tccttcctct atttcacgga tttggattgg
     1681 ggatttgtct tcattcggtt ttgttaggag gcggtcgttg tattctaatt cctcgtttta
     1741 gcaaaaagga atttattgat gccatcgcca agtgtaaacc aacctatatc gctggcgtac
     1801 ccacccacta tgaggcattg attaacagcg aaaagctgca aaaagctcaa ttttcttacc
     1861 taaaaggagc tttttgtgga ggagatagcg ttcctctttc tctcatcaaa cggttcaatg
     1921 actttatgca aagtcatgga gggatagtta gtttacgaga ggggtatggc ttaaccgaat
     1981 gtgtaacagc ttgttcgatc atgccggagg atatttataa agaaagaagc gttggtttac
     2041 ccttgcctgg aaatcgtttt aagattgttc gccctcaagg catagaagaa gtaccagtgg
     2101 gagaagtggg agaaatctgc atcaatggac ctaccgtgat gttgggatat aataatcggc
     2161 ctgaggagac ggatcgtgtt ctgcaaaagc acaaagacgg tctgatctgg ctttcgaccg
     2221 gagatttagg aactttagat caggacggtt ttttgtactt tactggaagg atgaaaagaa
     2281 tcattaagtg ctcgggctat gcagtctatc cctctcaggt agaaagtgtt attaatagtc
     2341 atccattggt tgtcgaatca tgtgttatcg gtattcctga tgaatacacc atgagtcgag
     2401 tcaaggctta tgtggttatc aggggcaatg ttcattctcg ggaaaaatta gctgaggaaa
     2461 taaaacaaag cgttgctgag cagcttatta agtggtcagt tccaactgag attacttttt
     2521 tggatagtct ccccgttacc cgcatgggaa aagcggacta tcgagcattg gaagccatgg
     2581 agcggggaga agcaattatt tgattttctt ttaaaaggat ttattttttc accccattga
     2641 taaatctatt agatagtggg cataggtcag gatgaggata taattacatt ttttcctatc
     2701 cgacgaggat tcataaaaaa tgaaatgtgt tgaaaagtat gatttctgca tggatacggt
     2761 tgtttatcaa aaagtatatg gaaaaaatgc cgaagaggct attgatgaag caacgaggaa
     2821 aatccgatat ttggaaggga aactcagttt tttccaagaa gaaagtgata tttttcaaat
     2881 taatattcat gcaggcaaaa actgggtgaa agtaagtcaa gataccattg atgttttgca
     2941 aaaagctgtt gaagtatcga aatcaagcaa tggtgctttt gatgtaacgg tcggagtttt
     3001 gactgattat tggcgccaat taaaaaaaaa tcaaaaaaaa ccggataaaa accaaattca
     3061 agacttgatt agtttgatta actaccggga tattttaatt aattcagaag aaagattagt
     3121 taagcttata cggttcaatc aaaaaattga tctgggagga ataggaaaag ggtatgctgg
     3181 ggagaaggtt atcgatgttt atagagatta ttcaatccag tcaggattta ttaatttagg
     3241 aggaaatgtt gtaaccattg gaaaaaccca ttggaagaga agttggatta ttggtattcg
     3301 taaccccttt tcggcggatg gggggatatt cgggaattta aaggtagaag aacaagcagt
     3361 tgtaacctca gggggatatg aaagatatta tgaaattgat ggtttaaagt atcaccatat
     3421 tcttgatcct cgaactggtt tccctgctcg gttagaatgg caaagcgtta ccatggttgc
     3481 agaaagctcc accttggcgg atgccctttc cactgctatt tttatcatga atcggcaaga
     3541 actcattcgt tttttgaaga attttccatc tttaggtgtg attctggttg cctatactgg
     3601 tgaaataatg atcagtaaaa atttaaaaga aatttttcaa aaaaccaacc cagaaatgtc
     3661 tataatatat attgattgag aaaaaatatt ttaaaaaata ggagggaaag tattatgggt
     3721 acgttagcaa ttgttcttat cattgtggct gtcattttgg taatcatctt tatttttgcc
     3781 tcagcaggac taaaaaaagt aggaaagctc aatatcggaa aaatcgatct ttccaaattg
     3841 gctgatggag tctatccagg tgaatttaag ggataccggt ggtcgaatca ggtagaggta
     3901 acagtgaaaa atcaccaaat tgccggaata aaaattatca aagatattgt ctttggatcg
     3961 ggagatattt ccaaaaagat cttcgatcag attattcaaa agcaatcgtt gcagatagat
     4021 acagtatcaa gtgcaacagt gactaataaa gcttacctaa aagcaataga aaacgcattg
     4081 ttgaaaggga aatagtaggg ttgagaaatt ataattctta aattcctatt tgtaaataag
     4141 aatctcattt tttaatatat ttcataaaag acaaaaagat aaaaagatga gatcctcacg
     4201 cagaaaggca ccgctcagga tgacagaatt ttttaaatcc tttgtagggg cttgatttat
     4261 catgcccgtg gttttcagga tagaccctca tgatgactag cagcaccaga tgaggataga
     4321 attatacacg ttccctgcta ttaatcactg gtcacagcat tcaaaaaacg gaataatgaa
     4381 gccttcatga gaaagaaact gcttaaaata atgatatttt aaccccaaca ctaaaaataa
     4441 aagaataatt cctatattta tgggtataat ttatcagtta ggatagaatg ccaattattt
     4501 tcaaccgaat atgttagaat aatttaaata agtatatagg agggctaaat atttatgaaa
     4561 cacaaaggaa tttttttcat cacattaatt ttactggtat tttcgttttc ggttatggtt
     4621 agtgccaaag taagcctgag tgctcttttt atgaagcaag ccggctatca agaaagcgat
     4681 atgatagcca tgacggatga atttttaaaa caaaatccag atgtcgaagt taatttaaac
     4741 tttgtatcct acgaagaact ccacgataaa attgccgtag ctgctgccag tgaatcggga
     4801 gtatacgatg tcattattag tgactgtgtc tggccggcgg aatttgcaga ggcaggatgg
     4861 attattgatg ttactgatcg tttaaaagac gaagaccgag ccgacatctt tccaaccgtt
     4921 ctgaattcag tgacctataa agatagaatt tatgggatgc cctggctcaa tgattggcag
     4981 tatttttttt ataacaagaa aatgttaaat gaagttggtg cttcagttcc tactttatgg
     5041 cccgaggtga ttgaaacttc ccaaaaaatg aaagccaatg gaatcatcga atatccaatt
     5101 attgattcct gggggcaagg agaggtagta gttgttcagt atttgaccta tttaaatgcc
     5161 atgggtggga aagcctttga tgaaaatggt ttgccgattt taaaccaagg aaaaggcctg
     5221 gatgccttga gctatatggt ggataattta aaagctggaa tttataatcc agcttcaatt
     5281 gaaagtttta acgaagaggt acggcgaatc tattcttccg gtcaggcagc ttttggtctg
     5341 aactggattt atatgtataa tttatcaaat gatccacaag agtcacaaat tgccggagat
     5401 tctgaaatag cattaatacc cggttttccc gatgggaaca agagtgcttc ctgtaatggt
     5461 gggatgggtt tgagcatagt gaatacctgc aaaaatccag atgtagcatg ggattatata
     5521 agtttcttat gcagtcgtga tatacagaaa aaatacagtg ccaacgctct tcccatttgg
     5581 atatccctct atgatgatcc agagttaatc gctcttcaac ccaaagctat ccctattgca
     5641 aaggaacaaa ttcagtatgt gtttgatcgg ccacaacttc cttggtattc gcaattttcc
     5701 caattgttag gagaagagtt gcaatcagct attactggag ctaagattcc tcagcaggca
     5761 cttgatgatg ctgttgcacg gatgaaagaa attcaagaaa gatattagtc tattgaatgc
     5821 aacctaaaca gcagggatta atactcctgc tgtttaggtt gcagattgaa ttaactatga
     5881 aatcaaacca gtacaaccga agagaagtca gatttgcctg gctattaatc attcctgctt
     5941 ttatttttat tggaattatt attttttatc caatggtgat ggctttctat accagtcttc
     6001 atcaggttga cttaactcga aaagcccagg gaatgccctt tattggtttt aaaaattata
     6061 ttgatatatt gaaaagcagc ggattttggg cctcaattaa ccgcactgca tattttaccg
     6121 tagtatctat tgtcattgaa atggtaattg gttttttaat tgctcttctt ctaaaccaaa
     6181 aattttgggc acgagggatt ctccgcagtt tattgttggt tccatgggca cttccgatta
     6241 ccgttgatgc tattatgtgg aaatggatat ttaatgctaa ttatggagcg tttaatgctc
     6301 tactcaaaca aattggttta attgattctt accgaggttg gttaactcgt ccgtggagtg
     6361 ctatgcattg cgttattgta gctgatgtat ggaaggttac accattagtt gccctgttgc
     6421 ttttggcagg attgcaaacc atacctcgag aaatgtatga agctgctact gttgatgggg
     6481 cgagttggtg gagaagcctt ttttccatta ccttgcctct attaaaacct acgctaactg
     6541 tagttttggt aatgcgtacc ttagatgcct ttcgagtttt cgatatcgta tatattatga
     6601 cttcgggtgg acctgccaat gcaaccaaag ttatttcctt tatgacctac caagaagctt
     6661 ttaaattttt aaattttggt cgtggttctg ccttggctta tctcattact ttaatagtgg
     6721 ctcttttggc ttatatttat accatcaaca tcgggaaaca tatcgaatat taaaaaaaga
     6781 gtattttata attcttttaa acggatttta ttcaggagtg aataaatgat tgttaaaaag
     6841 tataaaatat accgaacaat tggtatcacc gttgggtcaa ttatcttagt agcaggctta
     6901 ttagcaccct tagtttggtt gttgatatcg agtgttgctg atttaaaaga tttgctaaaa
     6961 attccactgc aatggatacc ggaaaacata tcctttgaaa gatattcaaa gatacttttt
     7021 tcccagggtt ctgaaagtga attccggaaa acaatgatga atagttttgt ggtagcttct
     7081 gcagttacca ttatttgtgt ttcaattggc tcgctctcgg cttatgcttt ttcacgactc
     7141 agatttcctg ggaaagataa aattttattt gtacttctct tttcatacat gcttcctcca
     7201 gtggccatca ttatccctat ttatcaaata tttagtcgat ataatttgct cgatacgaaa
     7261 acagctttaa tactggttta ttctgctatc atcactcctt ttgtgatttg gataatgcga
     7321 acttattttg atagtattcc acgggatttg gaagatgcag ctaaaattga tggatgcact
     7381 tatttaggta tgttatttcg aattatgatt cctctttcag cgccagggat tgtagctact
     7441 ctccttttgg catttttaat gtcttgggaa gagtttttta tggcattaat tatgacttca
     7501 tcggttgtat caaaaaccat tcccatatcg attgctgaat tttccggccg ccattcaatt
     7561 gattttggga tgatggctac cggtggaata ttagcagcaa ttccaccggt tataattgct
     7621 ttgattttcc aaaaatatat cgtcggtggt ttactatccg gagcagttaa ggggtagaaa
     7681 gcataattaa cataattgcc tggtagcgaa aaaccgctct aaaaaaattt tgttttaatg
     7741 aatgtggacc ttcatgctat agtatgacgc cagatgaaga tgaaaaccaa ggattcgaca
     7801 tgccatggca tgtcgctaca ttatatgaag atcgggcaca ataaattgca ccccaacatt
     7861 ttataatctt ttgtaggggc ttgatttatc aagtccattc caaatccgtc attgcgagga
     7921 gtctaaccgt tttttggtta gatgacgtgg caatctcatt tagaaaattt ttataaaata
     7981 attaaaggat gagatactca cgccctcaat aagcgtgggc ttagaaagac gtatcactat
     8041 agttccttct cccttgatgg gagaaggtga ggatgagggt gtaaactgag attcaaatcc
     8101 aggttaattg ctttaaaatt aggtattgag ggatatattg agtatccacc tcaccttaat
     8161 cctcttccac cagggaagaa aaaaatgaaa agagtgatta tggaaatttg gagggatatt
     8221 tttatatttt ttactatatc aacggtagcg gcttgagacc gatctaagga agtccccggt
     8281 gttttaagcc ggtaagatgg agaacaggaa cgctaaaaag gattccagta ccgggttcat
     8341 caagccttcc ggcttcgatc agtgcttttt gaatagcttc aacatgctct ttttcggcta
     8401 atatgataat aacttctttt gaggattcca catggaaact taaaaaatgc tttatatcat
     8461 tttcggcagt ggttcctcga ccataaagta tagtggcgcc tttagcgcca gcttttttag
     8521 ccttttctac caggtggtcg gctttgcctc gctcaaccac agcaaatatg gcgatgacat
     8581 ccattataac acctcatttc atgctttagt aataattcca agcatcaaaa ccgcaataat
     8641 ggttcccagg gaactcaaac cgacaattcc aaaaccattg agtagtgggt cgcttccttc
     8701 aactactcgg gataagccaa gagcaagggc aacgttgatg ggaatattta ccggtccggt
     8761 ggtggcactg gcgcagtcga atgcgatacc aaccattttt tcgggagcta aataagccag
     8821 gtacaaagca aataataaaa gggggattac aattttaaca gtcggtatat tgctaacaat
     8881 tttaataata cctagggtta ctcccaagcc aaatccgata gcaatagtat gcaaaaggac
     8941 tttttctgga atagctccta ctgatacttc ttccacttct aagaccagag tacgtaatgc
     9001 tggttctacc agggttgccc cgtagccaac cacaaatgca aagattgaga ttaataattt
     9061 attgtccaag gtcacaatgt tttgacctac cgattcacca agtggaatta gactcatggt
     9121 aactccctta agaaagagga agagaccgac aatggcaagt acgataccag aaaaaaaagt
     9181 cttagggtga gctaagggtt gtcttaacac tactacttgg aaaaaaacca atacggagac
     9241 cactgggata atacttttaa cgatgtcaaa aatatcatga aacagatagg taattctcaa
     9301 cttcagtcct ccccaggtga cagtgatgaa ataaaaatta taatgcctgg ttagtgatta
     9361 tctcatagta tttaatacaa agacggttag atactttcca tttttataat aacacagtac
     9421 aagagttcta gtaaggttca ttttaatgcc gttgaatttt aaaaattata ttattaattg
     9481 ctcagtgata tctaatatgc ataattacct tacccacgat ctggatatcg gggcttttag
     9541 ttggaacaaa gatcggcggg taagctggat ttgaaggtag aagaagaact aaaatcatct
     9601 taaaaaattg ttttaggaga taagccttca tgccacggta tgacaccaga tgagaatgag
     9661 aataattacc ccctcacttt aatccttttc caccaaggga gaggaaataa taaaatgact
     9721 ttcccgctag aaaagaaaat gggcaaagcc ttaaaatgac agcgtttaaa ccattactaa
     9781 atttaaaaaa tagcctataa attttgcgaa aaattaccac aaaaaaagcc tggcttttta
     9841 ttaaagccag gctcggaaaa gaataaactt ttttattctg cagcagcttc ttttttgatt
     9901 tcgatagatt ttaaaattgc ttgaatcttt tcatcggttt catgttctgg gcccattata
     9961 ccgattgaga ttttctcgcc atttaataga
//
LOCUS       DAOSOL010000010         9936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6780_length_9936_cov_4.150592, whole genome shotgun sequence.
ACCESSION   DAOSOL010000010 DAOSOL010000000
VERSION     DAOSOL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6780_length_9936_cov_4.150592"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PK171_00570"
     CDS             complement(<1..488)
                     /locus_tag="PK171_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOT04728.1"
                     /translation="MGLAVFATIAGLIAVIYAVYLIMKINTFDAGNERMKEISHAIQE
                     GAMAFLKREYFFLVFFVIGMFILLTFTRGIVEAICYLCGSICSVLAGFVGMRVSTRSN
                     ARTTQAATQGIGQALKVAFSSGTVMGMCVAGFGLLGLGILYLIVRDADIINGFALGAS
                     SIA"
     gene            complement(524..1591)
                     /locus_tag="PK171_00575"
     CDS             complement(524..1591)
                     /locus_tag="PK171_00575"
                     /inference="COORDINATES: protein motif:HMM:NF002327.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOT04729.1"
                     /translation="MEIATIKRFWENWEAQTLSPLATLSTKSRGREHPEEECELRNCF
                     QRDRDRIIHSKSFRRLKHKSQVFLSPEGDHYRTRLTHTLEVSQISRTIARGLRLNEDL
                     TEAISLGHDLGHTPFGHAGEEALREIHPGGFKHNEQSLRVVEFIERDGKGLNLTWEVR
                     DGILRHSKTRDLNMEKVSKENLPQTLEGQVVRWSDMIAYVNHDIDDAIRAGVITQDDL
                     PQDCLEVLGKSHRERINRMVKDVILNSVEKSPVTMSIPVRNATQELRKFLFDTVYFGE
                     YQQLEREKAKRLIKELYQFFLENLDIFEKEYRVNFYHQDTDIIVCDFISGMTDRYAMA
                     IYRKYFIPFSWPQEGMGKEIN"
     gene            complement(1656..4328)
                     /gene="ppdK"
                     /locus_tag="PK171_00580"
     CDS             complement(1656..4328)
                     /gene="ppdK"
                     /locus_tag="PK171_00580"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOT04730.1"
                     /translation="MKKYVYFFGEGDASMKMLLGGKGANLAEMTRIGLPVPPGFTIST
                     EACHHYYKNNETWPEGLEEEVKKNLIELEKKSGKKFGDPVNPLLVSVRSGAPASMPGM
                     MDTVLNLGLNESSLQGLVKLTGNERFALDCYRRFIQMFGDVVMGVDHDKYEEILSDVK
                     KQVGVNLDTDLTAEDLKVVISRYKELYKKVTGEDFPQEPFDQLKKSINAVFSSWNNKR
                     AITYRKINKIPENWGTAVNVQMMAFGNMGNDSGTGVGFTRNPSTGIKEFYGEYLLNAQ
                     GEDVVAGIRTPKPIKDMGQDLPEDFEELKKIYSILEEHYRDMQDFEFTIEKGKLYMLQ
                     TRTGKRTTQAAIKIAVDMVSEGLIDKKTAVKRIDPASLNQLLHPQIDRSIPLKVIAKG
                     LPASPGAASGKVYFDADDAEREANEGTKAILVRPETTPDDIHGVVAAQGVLTSRGGMT
                     SHAAVVARGMGKPCVAGCEAVVIDLDKKQFQVNSLVVKEGDYISIDGGKGEVILGQAK
                     TIPPQLSEDFQKLLGWADSIRKLGVRANADTPNDARKAIEFGAEGIGLCRTEHMFMAP
                     DRLPAVQEMIIAQNVEERKKALAKIEPMQKGDFKEIFKVMEGKPVTVRLIDPPLHEFL
                     PKLEDLLVEVTTLRVKGISGSELEEKEKMLKIVRGLHEVNPMLGLRGCRLGIVYPEIV
                     EMQVRAIIEAACELDKEGVKVLPEIMIPLVGHQNEIKIQKEILEKVAKEALDRHQSKV
                     QYKFGTMIEIPRAAVVADQIAEYAEFFSFGTNDLTQMTFGYSRDDAEGKFLFFYEENG
                     ILPEDPFQSIDLDGVGELMKMGVQKGRQVRPDLKCGICGEHGGDPKSVMFCHTIGLNY
                     VSCSPFRVPLARLAAAHAVIEEEGKVKDTSKFDK"
     gene            complement(4325..5185)
                     /locus_tag="PK171_00585"
     CDS             complement(4325..5185)
                     /locus_tag="PK171_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HOT04731.1"
                     /translation="MSSEFCDIKTSDDFSIFVVSDGTGKTAYLAVQAALAQFDLPRVK
                     ILRFDHVKNKEEIFSILDITRPQKDLVIYTLVNDDFVQALVSESLHRRILVLDLLGPI
                     VNSIRKLSTRSPRGEPGLLHGSNRESVAITEAVEYALAESTGVSFSRLNEAQVIILTV
                     WFPHRDDCIMQLAKKGIKCGYMMLNPDLPLPQNMEDILGKDPGKIVIGFIMEPELLQK
                     LRNERIMNLGLNYMEYKANQEVVRQEIKFAQDIYQKLNCPVIDITSLNSKDIINQILL
                     KIQKQREEAE"
     gene            complement(5182..7287)
                     /gene="glyS"
                     /locus_tag="PK171_00590"
     CDS             complement(5182..7287)
                     /gene="glyS"
                     /locus_tag="PK171_00590"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOT04732.1"
                     /translation="MTDKRDFLLEVGVEELPSSLIPSIFEETKSRFIEKLKESRLHYE
                     DLYLVGTPRRVALIIKGLQEHQEPLIMKVKGPAAKVGFSPDGKALQPALAFARAQGID
                     PQDLFIKETERGKYVFGKKVRVGQPTKEVLIQICPIILRSLNFPRSMLWGEGNYRFVR
                     PIRWLVALFGEDEIPFSVAGVSASRFTHGHRFLSKNSQVLHKSTDYIPVLGSLKVIVD
                     PAQRRTMIEEALDREAKKIGGHWLKDEDLINEVTFLVEYPDAALGNFNEKYLKLPACV
                     LTTVMKHHQKYFACVDDEGKLLSRFLVVLNRPAFGCEKIIHGNERVLKARLEDALFFF
                     TEDQKKNLEQRTKFLRGIVFQEGLGTMWEKTLRLKFLVKRLGACFPGNEDSLTHLDRA
                     ALLAKADLTCEMVKELPELQGHMGKVYAQLDHENDEVAIAIKEQYLPSPGQEDYPETF
                     TGSILSLADKMDNIVSSFSLGRVPSGSTDPFGLRRQAQGIFNILLNRRWYSNLREWIG
                     WNLEILKEQGFVNPDPSVIDDVIQFIVGRFRYYLLETGMNYSIINAVLSASCNDIYDI
                     YLRIHVLQKLFDNKRKFFDDIITGFCRANNITKNFQELPLVDTNLFEEEVERQLNNTL
                     QSVEKDFYPAIQRGDYIYACESFSTVIPVLNRFFDDVLVMTESELVRNNRLSLLKKIV
                     ELWQPIADLSQIVIEEESR"
     gene            complement(7301..8242)
                     /gene="glyQ"
                     /locus_tag="PK171_00595"
     CDS             complement(7301..8242)
                     /gene="glyQ"
                     /locus_tag="PK171_00595"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOT04733.1"
                     /translation="MTPIKATDILIPWCVFWVGDDTIYFQDIILTLSQFWKKNGCIIQ
                     QPYDIEVGAGTMNPATFLRVIGPEPWGVAYCEPSRRPADGRYGENPNRLYQHYQYQVI
                     IKPSPSDIQDIYLNSLYELGINPAEHDIRFVEDNWESPTLGAWGLGWEVWLDGMEITQ
                     FTYFQQAGGLDLKPISVEITYGLERITMYLQEKENVFDIQWNEILTYGDVRWQEEREH
                     SIYSFEHNGTDILFKLFESYEKEAQRLLEREMVMPAYDYILKCSHTFNLLDSRGGISV
                     LERSQYMGRIRQLANQCARLYYQQREKMGFPWLKKSN"
     gene            complement(8239..9006)
                     /gene="recO"
                     /locus_tag="PK171_00600"
     CDS             complement(8239..9006)
                     /gene="recO"
                     /locus_tag="PK171_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT04734.1"
                     /translation="MMKTGRYWKDQGIVLKSINFGELDRIVTLFTRKKGKINVIAKGA
                     RKVGNRFGPALDCPAISNFMFYNGRGMPVLSQAEIVDCHREIGKKTNQWVFANYLLFA
                     IDRCYEPEESDERIFETVLFYLDLSTKCENFYILALKFRIDLIRFLGFLPRIRSCAVC
                     GKPFKKIPQGWSVASGGMICEKCFSSIADIHYLPPNMMTLLAKLMTFSCASCLSIQLR
                     ENQFAYLDRFVSEYLTYHVGKKITEWKIFLERFQEAS"
     gene            complement(9003..9899)
                     /gene="era"
                     /locus_tag="PK171_00605"
     CDS             complement(9003..9899)
                     /gene="era"
                     /locus_tag="PK171_00605"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT04735.1"
                     /translation="MEDRASFRSGFVTIWGRPNVGKSTLLNSILKYKITIVSDKPQTT
                     RKNIKGILNHPDCQIVFIDTPGLHVPVDPLGEFLSNQWKSSLFDADCLLYLTNPGVSV
                     DTDEIYLKEIRNFPHPILLVVNKIDLFKKGTIQKTIHEFSQHFSFHDILEVSALTGVG
                     LEQLIQKIRDLLPISPPYFDEEQISDVYERDIVGEMIREKVWHHIHQEVPYGVEVRVE
                     EFKEKENLVSIRAIIYVEKDSHRKIIIGGKGSMIKRIGSDARKDLEEFFGVKVFLDLW
                     VKTQLNWRKKPAVLRRWGYTIR"
BASE COUNT         2914 a         2217 c         1766 g         3039 t
ORIGIN      
        1 gcgattgaac tagcgcccag agcaaaaccg ttgataatat cagcatctcg aacgatcaga
       61 taaagaattc ctaaccccaa aagtccaaaa ccagctacgc acattcccat aacagtacct
      121 gaggaaaaag ccaccttgag agcttgacct attccttggg ttgcagcctg cgtggttcgg
      181 gcattgcttc tggttgaaac cctcattccc acaaaaccgg ctaatactga acaaatcgaa
      241 ccacaaagat aacaaatggc ttcaacaatt cctcgggtaa aagtgaggag gataaacatt
      301 ccgataacaa aaaaaaccaa aaagaagtat tcccttttga gaaaggccat agccccttcc
      361 tgaatagcat gggaaatctc tttcattctt tcattaccgg catcaaacgt attaattttc
      421 ataatcaaat aaactgcata tataaccgct attaaaccag ctatagttgc aaatactgct
      481 aatcccacaa tataatcctc ccttctgaat tactactatt cattcagttg atttcttttc
      541 ccattccttc ctgcggccag gaaaatggaa taaaatattt ccggtagatg gccatggcat
      601 atcgatcagt catccccgaa ataaaatcac atacgattat atcggtatct tggtgataaa
      661 aattaacccg atattccttt tcaaagatat ctaaattttc caaaaagaat tgataaagct
      721 cttttattaa tcgtttcgct ttttctcttt ctaactgttg atattctcca aaatacaccg
      781 tatcaaatag aaactttcgt aattcctggg tggcgttccg aactggaatg ctcatggtca
      841 ctggagattt ttcaacgctg ttaagaataa cgtctttcac cattcgatta attctttctc
      901 gatggctttt ccccaaaact tctaaacaat cctggggaag gtcatcttga gtgataacac
      961 cagctcgtat agcgtcatcg atatcgtggt ttacataggc tatcatatct gaccatctaa
     1021 ctacttggcc ttccaaggtt tggggtaaat tttctttgga aactttctcc atatttaaat
     1081 ccctggtctt ggaatgccga aggataccat ctctcacctc ccaggtcaaa tttagacctt
     1141 ttccatctct ttcgataaac tcaacaactc gtaaactttg ttcattatgc ttaaaacctc
     1201 ccggatgtat ttcccggagg gcttcctcgc cggcatgccc aaaaggggta tgccccagat
     1261 catgaccaag gctgatcgcc tcggttaggt cttcattcaa ccttagccct cgagcaatag
     1321 tccgagaaat ttgcgagact tccagggtgt gggtaagccg ggtacgataa tgatcgcctt
     1381 ccggacttaa aaaaacctgg cttttgtgct tgagtcgtcg aaaagacttg ctgtggatga
     1441 ttcggtcacg atctctttga aagcaattcc gtaattcaca ttcttcttca ggatgctcac
     1501 gacctcggct tttggtactc aaagttgcca gtggcgagag ggtttgagct tcccagtttt
     1561 cccaaaatct tttgatcgta gcaatttcca agcgatcacc tgaaaaaccg gactggtata
     1621 taccagtccg gtttatttcg gttcaataat tatgtttact tatcgaactt agatgtatcc
     1681 ttcacttttc cttcttcttc gattaccgca tgagcggctg ccaaacgagc caaaggaaca
     1741 cggaaaggtg aacagctgac atagtttaat ccaatagtat gacagaacat aaccgacttc
     1801 ggatcaccac catgttcacc acagatcccg catttgaggt ctggtcggac ttgtctccct
     1861 ttttgaacac ccatcttcat taactcacca actccatcaa gatcaatgga ttggaaagga
     1921 tcctcgggta gaataccatt ttcttcatag aagaaaagga acttgccttc agcgtcatct
     1981 cggctgtacc caaaggtcat ctgggttaag tcgttggtcc caaaggagaa aaactcagcg
     2041 tattcagcaa tctgatcggc gactactgct gctcgaggga tttcaatcat ggttccaaat
     2101 ttatactgaa ctttcgactg atgtcgatcc aaagcttctt tggctacttt ttccaaaatt
     2161 tctttttgga ttttgatctc gttctggtga cctaccaatg gaatcataat ttctggaaga
     2221 acttttactc cttctttgtc gagctcacaa gctgcttcga tgattgcccg aacctgcatc
     2281 tcgacaattt ctggatatac tattcccaat cggcaaccgc ggagacccaa catcgggttg
     2341 acttcatgaa gtccacgcac gatcttgagc atcttctctt tttcctcgag ttcagaacca
     2401 ctgattcctt tcacccgtaa agtggttact tcgaccaaga gatcctctaa tttaggcaga
     2461 aattcatgaa gaggaggatc gattaaacga acggttactg gttttccttc cataacctta
     2521 aatatctctt taaaatcacc tttttgcatt ggttcaattt ttgccaaggc tttttttcgt
     2581 tcttcaacgt tctgagctat gatcatttcc tgtactgctg gtaaacgatc gggtgccatg
     2641 aacatatgct cggtccgaca caaaccaatt ccttcagcac cgaattcgat cgctttacga
     2701 gcatcatttg gtgtatcggc attagcacga acacccagtt ttcgaatgct atcagcccaa
     2761 cctaaaagct tttggaaatc ttcggaaagt tgaggtggaa tggttttggc ttgtcctaaa
     2821 atgacttcgc ctttcccacc atcgatactg atatagtctc cttctttgac gaccaaagaa
     2881 ttaacctgaa attgtttttt atcgagatcg ataactaccg cttcacaacc ggcaacacag
     2941 ggttttccca ttcctcgagc aactactgca gcgtgactag tcattccgcc tcggctggtt
     3001 aaaacccctt gtgcagcaac gacaccatga atatcatcag gggtggtttc aggacgaacc
     3061 aaaatagctt tagttccttc attggcttcc cgttcggcgt catcagcatc aaaatatacc
     3121 ttcccactgg ctgcaccagg tgaagctgga agtcctttag cgataacctt caacggaatg
     3181 cttcggtcaa tctggggatg aaggagttga ttaagagagg ccggatcaat tcgttttaca
     3241 gctgttttct tatcaattaa tccttcactc accatgtcta cagctatttt tatggccgcc
     3301 tgggtggttc gtttcccggt tcgggtttga agcatataaa gcttcccttt ttcaattgta
     3361 aactcaaagt cctgcatatc ccgataatgt tcttctaata tagaataaat cttcttcaat
     3421 tcttcaaagt cctcgggaag atcttgcccc atatccttaa ttggttttgg agttcgaata
     3481 ccagccacca cgtcttcacc ttgagcatta agcaagtatt ccccatagaa ttcttttata
     3541 ccggttgatg gattgcgagt aaaaccaacg ccagttcccg aatcatttcc catattacca
     3601 aaagccatca tttgaacgtt gactgcggtt ccccagtttt caggaatttt attgatcttt
     3661 cggtaggtga tagctctttt attattccat gaactaaaaa cagcattgat ggattttttt
     3721 aattgatcaa atggctcttg agggaaatct tcaccagtca cctttttata cagttcttta
     3781 tatcgtgaaa tgactacctt taagtcttca gcagtgagat cggtatcgag attgacacca
     3841 acctgttttt ttacatcact gagaatttct tcgtatttat cgtggtcgac acccataacc
     3901 acatcaccga acatttggat aaaacgtcgg taacaatcaa gggcaaagcg ctcattaccg
     3961 gttagtttaa caagaccttg aagagagctt tcattcaacc ctaaatttaa aactgtatcc
     4021 atcatgcctg gcatggatgc cggagcgcca gaacgaactg aaactaaaag cgggtttact
     4081 ggatctccaa atttttttcc tgacttcttt tccagttcaa ttaagttctt tttaacctct
     4141 tcttcaagtc cttcgggcca tgtttcattg tttttgtaat aatgatgaca ggcttcggta
     4201 ctaatagtaa atcctggagg gacggggaga cctattcggg tcatttcagc aaggttggca
     4261 ccttttccgc ccaaaagcat cttcattgat gcatcgcctt caccaaaaaa ataaacgtac
     4321 ttcttcattc tgcttcctcc ctttgctttt gaattttaag caaaatctga ttgattatgt
     4381 ctttactatt taaactggtg atatcaatca ccggacaatt taacttttga tatatgtctt
     4441 gagcaaattt aatctcttgt cgaacaactt cttgattggc tttatattcc atataattta
     4501 aacccaggtt cataatgcgc tcattacgga gcttttgtaa aagttctggt tccattataa
     4561 agccgatgac aattttccct gggtcttttc ccaaaatatc ttccatgttc tgtggaagtg
     4621 gaaggtcagg gttaagcatc atataaccac atttaattcc cttttttgcc aattgcataa
     4681 tgcaatcgtc acgatgggga aaccatacgg taagaatgat gacttgtgcc tcattaagcc
     4741 gagaaaaact tactccggta ctttccgcca gtgcatattc tacagcttca gtaattgcca
     4801 ctgattccct attggagcca tgaagcaaac ccggttctcc ccggggagat cgagtcgata
     4861 gcttccggat agaattaaca atcgggccca acaaatccaa tactaaaatt cgacgatgca
     4921 gtgattcaga aactagtgct tggacaaaat catcgttgac tagagtataa atcaccaaat
     4981 ccttttgagg gcgtgttata tcgagtatac taaatatttc ttctttattt tttacgtgat
     5041 caaaccgtaa aattttaact cgagggagat caaattgggc aagtgctgct tgtacggcta
     5101 aataagcagt ttttcccgtt ccatcagaaa caacaaatat actgaaatca tcactggtct
     5161 taatatcaca aaattcggag ctcacctgga ttcctcctca ataacgattt gagaaagatc
     5221 ggcaatgggt tgccagagtt caacaatctt ttttaataat gataatcgat tgttgcgcac
     5281 taattctgat tcggtcataa ccaacacatc atcaaaaaaa cggttgagca ccggaataac
     5341 ggttgaaaaa gattcacagg cataaatata atcgcctcgt tggatagctg gataaaaatc
     5401 tttttctacg ctttgcagtg tgttatttag ttggcgttca acttcttctt caaagagatt
     5461 ggtatccact aagggaagtt cttgaaaatt tttggtgatg ttgttggctc gacaaaagcc
     5521 ggtaattata tcgtcaaaaa atttcctttt attatcaaat aatttttgaa gtacatgtat
     5581 tcgcaggtaa atatcataga tatcattaca agaggcactt aatactgcat tgataatgga
     5641 ataattcatt ccagtttcca ataaataata acgaaaccgg ccaacaataa actgaattac
     5701 atcatctatc accgaaggat cgggatttac aaatccctgt tcttttaaaa tttctaagtt
     5761 ccaaccaatc cattctcgca agtttgaata ccaacgacgg tttaacagaa tattaaaaat
     5821 accctgcgct tgtctcctta atccaaaagg atcagttgaa ccgctgggta ctctccccaa
     5881 agaaaaactg gaaactatat tatccatttt atcagcaagg gaaagaatac ttccggtaaa
     5941 agtttccgga tagtcttcct gccccgggga gggaaggtat tgttctttaa tagcaatggc
     6001 aacttcgtca ttttcatggt ctaattgagc ataaaccttt cccatatgac cctgcaattc
     6061 cggtaattct ttaaccattt cacaggtcaa atcggctttg gctagaagag cagcccggtc
     6121 taaatgggtg agagagtctt cattgccagg gaaacaagcc cccaatcgtt taactaagaa
     6181 ttttaagcgc agtgtttttt cccacatggt gcctaatcct tcttgaaaaa ctattcctct
     6241 taagaatttg gttctttgct ctaaattttt tttctggtct tcggtaaaaa agaacaaagc
     6301 atcttcaagt cgagctttca acactctttc attcccatga ataattttct cacacccaaa
     6361 agccggtcga ttcaaaacca ccaaaaaacg agaaagcaat ttcccctcat catcaacaca
     6421 ggcaaaatat ttttggtgat gtttcatcac cgtagtgaga acgcaagcgg gaagttttaa
     6481 atatttttcg ttaaaatttc ccaaagctgc atcgggatat tctactaaaa aagtaacttc
     6541 attaataagg tcctcatctt tcaaccaatg accgccaatc tttttggctt ctctatctaa
     6601 cgcttcttca atcattgttc ttctttgggc cggatccact ataaccttga ggcttcccaa
     6661 aactggaata taatctgttg atttgtgtaa aacctgagag ttttttgata aaaatcgatg
     6721 tccgtgagtg aaacgagaag cagaaacacc agctaccgaa aacggaattt catcttctcc
     6781 aaaaagtgct accaaccatc ggatgggacg aacaaaacga taatttccct ctccccaaag
     6841 catggaacga ggaaagttta aagaacgtaa aatgatagga cagatttgga tcaatacttc
     6901 tttggttggt tgtccaactc tcactttttt cccaaaaaca tatttccctc tttcggtttc
     6961 ttttataaaa aggtcttgag ggtcgatccc ttgagcccgc gcaaatgcca gtgctggttg
     7021 aagagctttt ccatccggag aaaaaccaac cttggccgct ggtcctttta ctttcataat
     7081 caaaggttct tggtgttctt gtaatccctt gatgataagg gcaactcgtc ggggtgtacc
     7141 gactaaatat aaatcctcat aatggagccg tgattccttc aatttttcga taaacctact
     7201 tttggtttct tcaaatatcg aaggtatcag actcgaaggc agttcttcaa ctccaacttc
     7261 taaaaggaaa tctcttttat cagtcatgat ttaccttaac ctaatttgac ttttttaacc
     7321 agggaaaacc cattttttct ctttgttgat aataaaggcg ggcgcactgg ttggctaatt
     7381 gtcgaattct ccccatatac tgacttcttt ccaggacact gattccaccc cgagagtcaa
     7441 gaagattaaa agtatgagaa catttcaata tataatcata agcgggcata accatttctc
     7501 tttctaagag ccgctgagct tccttttcat aagactcaaa aagtttaaaa agaatatcag
     7561 ttccattatg ctcaaagcta taaatcgagt gctctctttc ttcctgccag cgaacatcac
     7621 cataagtgag gatttcgttc cactgaatat caaaaacatt ctctttctct tgtaaataca
     7681 tggtgattcg ttctaaacca taggttattt ccactgatat cggttttagg tccaaacctc
     7741 cagcttgctg aaagtaggta aactgggtga tttccatacc atccaaccac acttcccacc
     7801 ctaagcccca ggctcccaga gtgggagatt cccagttatc ctcaacaaag cggatatcgt
     7861 gttcagcagg attaatgcct aactcatata aactgttgag ataaatatcc tggatatcgg
     7921 atggtgatgg cttgataatt acttgatatt gatagtgctg gtatagacgg ttaggatttt
     7981 ctccataacg tccatcggct ggccgtcgtg aaggctcgca ataggcaaca ccccagggtt
     8041 ctggtccaat caccctgaga aaggttgccg ggttcatggt tccagcacca acttcaatat
     8101 catatggttg ttgtatgatg cagccgtttt ttttccaaaa ttgactgagg gttaaaatta
     8161 tatcttgaaa gtatatggta tcatcaccta cccaaaaaac acaccaaggt attaatatat
     8221 cagttgcctt tatgggtgtc aacttgcttc ttgaaaacgc tcgagaaata ttttccattc
     8281 ggtaattttt tttccaacat gataagtgag atactcagat acgaatcgat caagataggc
     8341 aaactggttt tctctcagtt gaatcgataa acacgaggca caggaaaaag tcattaactt
     8401 cgccaagaga gtcatcatat tcggaggtaa ataatgaata tcggctatcg aagagaaaca
     8461 tttttcacaa atcattccac ctgatgcaac actccatcct tgaggaattt ttttaaaagg
     8521 tttcccacaa accgcacaag atcgtatccg aggcaaaaaa cccaaaaaac gaattaagtc
     8581 gatacgaaat tttaaggcta aaatataaaa gttctcacac ttcgtagata aatcaagata
     8641 aaagagaacg gtttcaaaaa tcctttcatc ggactcttcc ggttcataac aacgatcaat
     8701 tgcaaataaa agataattgg caaaaaccca ctgattggtt ttcttgccga tttctcgatg
     8761 acaatccaca atttccgctt gagaaagaac cggcattcct cgaccattat aaaacataaa
     8821 attcgaaatt gccgggcaat caagagccgg accaaatcgg tttccaacct tcctagctcc
     8881 tttggcaata acgttaatct tccccttttt tcgagtaaag agggtgacaa ttctatctaa
     8941 ttcaccaaaa tttatacttt ttaaaacaat tccctgatct ttccagtaac gaccagtttt
     9001 catcatcgga ttgtataacc ccatcttctt aagactgcgg gtttttttct ccaattcagc
     9061 tgagttttaa cccatagatc taaaaacact tttaccccaa aaaattcctc caagtctttt
     9121 ctggcatctg aaccaattct tttaatcatg cttcccttcc ctccgattat aattttacga
     9181 tgggaatcct tttcaacata aattatagcc cgtatagaaa ccaaattctc cttctcttta
     9241 aattcttcaa cccgtacttc caccccataa ggaacttctt gatgaatatg atgccaaact
     9301 ttttcacgaa tcatttcccc aacaatatct ctttcataga cgtcagatat ttgttcctca
     9361 tcaaaatagg gtggactgat gggaagaagg tcgcggattt tctggattaa ttgttccagt
     9421 ccgacacctg ttaaagctga aacctcaaga atatcgtgaa aagaaaaatg ttgagaaaat
     9481 tcatgaatgg ttttttgaat tgttcccttt ttaaataaat caattttatt cacgactaat
     9541 aggatcggat ggggaaaatt tcggatctct tttagataga tttcgtcagt atcaaccgaa
     9601 acaccagggt tggttaaata gagcaaacaa tcggcatcga aaagcgaact tttccattga
     9661 ttggaaagga attcgccaag ggggtcaacc ggaacatgaa gcccaggggt atcgataaaa
     9721 acgatttgac aatcggggtg atttaaaatt ccttttatat tttttcgtgt ggtttgaggt
     9781 ttatcactga caatggtaat tttatatttt aaaatgctat tgaggagagt agatttccca
     9841 acatttggtc tcccccaaat ggttacaaaa cctgaacgaa agctagcccg atcttccatg
     9901 gcttatctcc ctttttcggg attgaaccga aaggga
//
LOCUS       DAOSOL010000011         9899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6816_length_9899_cov_4.785351, whole genome shotgun sequence.
ACCESSION   DAOSOL010000011 DAOSOL010000000
VERSION     DAOSOL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 9899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9899
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_9899_cov_4.785351"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PK171_00610"
     CDS             <1..956
                     /locus_tag="PK171_00610"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT04736.1"
                     /translation="TIHNLAYQGIGSSELFKIVNLPGHFFTHDYLEFYGNLNLLKAGL
                     VFSDVITTVSPSYAREIATPEGGEGLDGLIRAIGLRKPIIGIVNGIDTDLFNPATDKN
                     LPYPYTNGQLEIKKQNKLAFYQQYFPDLKEKDALFPLISFISRLVEQKGLDLILNDPD
                     KFFNLPLCWFFLGVGEAAYENSLTEYAKKYPNLHVEMAFNNSLARFAYGASDMLAMPS
                     RFEPCGISQMIAMRYGTIPVVRKTGGLIDTVIDYRFNPVLNNGFQFDNYRPDEFIFTL
                     ERALNWYNDTPELWKVMVKNAMDSDFSWKIPVQEYLNIYLG"
     gene            969..1604
                     /gene="coaE"
                     /locus_tag="PK171_00615"
     CDS             969..1604
                     /gene="coaE"
                     /locus_tag="PK171_00615"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT04737.1"
                     /translation="MIKIKRSVPIIGVGGATGSGKSSVAHSFSTHNAMIIDLDRLSHD
                     LSQKGRRLWKAVVLAFGSGFLNQKGELQRKKLGRVIFKSWRLLFLLNRISHPIFKNEI
                     KKIILHQPPSAMIVIDGAILFEAGLIPLVDCLIFVEAPEEQRSVRLSQKGLSPDDIRS
                     RMKSQKFISCLRRRSHIIIENSATPEDLQQKINFVCQLPIEKLIQSDRKTG"
     gene            1549..3669
                     /gene="uvrB"
                     /locus_tag="PK171_00620"
     CDS             1549..3669
                     /gene="uvrB"
                     /locus_tag="PK171_00620"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT04738.1"
                     /translation="MSVSFPLKNLSNPIGKPGDIVPTKGRFQLVSDYQPQGDQPTAIE
                     NLNKGISNQCRFQTLLGVTGSGKTFTMANVIEHYQRPTLIISPNKTLAAQLYSEMKAF
                     FPNNAVEYFVSYYDYYQPEAYVPAQDLYIEKDASINEQIDRLRLSATSALMERNDVVV
                     VASVSCIYGIGSPEEYRKRIFPVRVNDTWNRDEFAHRLVDLLYERNEIEFISGCFRMK
                     GDTIEVYPAYSESALRFEFWGNQVECIKIFEPVSGKTLNKLSEIFVYPAKHYLVDRDQ
                     FDLALRNIELELQDQLKSFLSQGKLVEADRLERRTRYDLEMLRETFYCSGIENYSRHL
                     DGRRPGEPPYTLIDYFPRDFLLFIDESHVAIPQIHGMLAGDRSRKKALVDFGFRLPSC
                     FDNRPLSFEEFEAKLGYVIYVSATPGTYEYQKSDQIVEQLIRPTGLIDPEIIVRPATG
                     QVEDLYQEIKPVIQSGERVLVTTLTKRSAEDLSDYLFNLGIKVRYLHSEIETIERARI
                     IRDLRAGEFDVLVGINLLREGLDLPEVALVAVLDADKEGFLRSEVSLIQTIGRAARNI
                     KGRAILYADRLTGSLERAMQETERRRKTQLEYNRTHHITPESIIKEVRTLIPEMVGLS
                     PEAENTLYQLDQLETDRVAEEDLIEKLTQEMKKAAKNLEFEKAALIRDELIRIKKTVF
                     QTDQKNIRNQQKTASRISGKSHSN"
     gene            3707..6517
                     /gene="uvrA"
                     /locus_tag="PK171_00625"
     CDS             3707..6517
                     /gene="uvrA"
                     /locus_tag="PK171_00625"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT04739.1"
                     /translation="MIVLINSLLIKGAREHNLKNIDIEIPHHKLVVITGLSGSGKSSL
                     AFDTIFAEGQRRYLESLSSYARQFLEKMEKPDVDLIEGLSPAIAINQKASSHNPRSTV
                     GTVTEIYDYMRVLFSRCGKVFCPQCGKPIARQTIQQIVDEILSYPDGTRIVILAPLVR
                     GRKAEYKKLLNDMLKNGFARSLVDGELIELEDWDSLNLDRNKKHNIDLVVDRLTTSSE
                     EQRRLADSIEIALRYGKGLVKIGLVADGMQMEERIFSEHFACPDCGISFPEVTPRMFS
                     FNNPYGACPDCSGLGILNFIDPGLVIPDWSKSLDQGAIIPWNRYDENHYSMNKIKRFL
                     RDKGIAFKVPFQNLSENDRQLILYGDEDGDGIISYLEKRMKAAESWWEMDEVARFMAT
                     RTCPSCQGNRLRPESLSVKIQNQSIGDLSRYTIGDLENFFITLKLSKQDELIAATLLR
                     EIQARLHFLVEVGLSYLTLSRSAMTLSGGESQRIRLATQIGSGLVGVLYVLDEPTIGL
                     HPRDNQKLIQTLKRLRDLGNTVIVVEHDAETMKNSDYLIDMGPGAGEKGGEVIAIGTP
                     VEIMCNQKSLTGQYLSGKKEIPIPLERKNPQEWLVIRGARANNLKNIDVKIPLGVMVG
                     LTGVSGSGKSTLMEDVLYRSLSRIFYHSLAEPGDHDTIEGIESIDKVIVIDQSPIGRT
                     PRSNPATYTGIFTEIRNLFAQLPESKARGYRPGRFSFNVKGGRCEACQGNGVIKFEMH
                     FLPDVFVTCEYCKGKRYARETLDVTFKGKNIAEILDMSVDEAAVFFENIPVVRRKLAV
                     LQRVGLGYIKLGQSATTLSGGEAQRIKLTKELSKIATGRTLYLLDEPTTGLHFADVEK
                     LLDVLLELRRRGNSILIIEHNMDVIKCCDYLVDLGPEGGDQGGQLVAEGTPEEVASNP
                     ASYTGFYLREVLKIERKEFCQV"
     gene            6489..8348
                     /gene="uvrC"
                     /locus_tag="PK171_00630"
     CDS             6489..8348
                     /gene="uvrC"
                     /locus_tag="PK171_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT04740.1"
                     /translation="MSEKNFVKSETTRNSLQELPTSCGVYILCNQNHQIIYIGKAINI
                     QNRVRNHLQGDSASPLKRDMAEEITTIQFILTRDEAEALLLEEELIKTYQPHYNIRMK
                     DDKSYPYIHFDARGDFPAVKIARRKTAKPGYFYGPYTNSKKARDGLKILRQIFLLRGC
                     SIPESRFPLKRSCLDYELKLCCAPCVWKGSPSEYQDKVKKARSFLEGDYLEVTNWLEE
                     EMWKAADRQDFEKAIQFRDRLDCLLKIIGRYRLVLPDSSDVDFIELENESNLASLVVV
                     KVRKGRLIGIENFHAQGEENADRNYILNEFIENFYLDHFNPPTKICVQFDYNRNLQER
                     IKEKGYSSIIELPRNTSELDLLHFAQENAQKNLQLEKIKALRQNLDHQHLLHELKNLL
                     SLPALPRLIEGVDISTFQGDESVGAVVAFFDGLPLKKRYRKYIIREADHPDDFAMIEE
                     TLTRHFNKLIQNQTELPNLLVIDGGIGQLNSALKVLQKFSLTIPVLSLAKENEEIFFP
                     GKSISLRLPPENSSLQFLQRIRNEVHRFVITFHRVRRNKKMFSSLLDDIPGIGPQRKQ
                     VVLSAFDNLTQILNYSPQELSRQLKIPSQSIIKIQKKISQKNMLAGKEGITSS"
     gene            8345..9253
                     /gene="whiA"
                     /locus_tag="PK171_00635"
     CDS             8345..9253
                     /gene="whiA"
                     /locus_tag="PK171_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOT04741.1"
                     /translation="MNFQESLKTELFTIQPQNKDEVMSEFIGLIRTGAVIKKHSHELL
                     LLFSSRHPALIKKCVTHKKSLFPHSSHTISVQERRGIPGGIFYCFDFSVTNIMLDQFG
                     FHDVNILSNYLNQKLAAHFLRGVFESRGYISNPMRGYHLEIQFNSEKLAHYILKYLHN
                     LQFDFRSRTVKGDINLYLKNSQLIADFIRFLGAHQSYLEMEKIMVEKSTINEITRWVN
                     YETANLNKIVASSSRQRKKIQVLDFDDLPFHLQEIARLRLENPCSSLREIGDLCNPPL
                     SKSEVHRRLNKIEKIADQKGFTENLT"
     gene            9457..>9899
                     /locus_tag="PK171_00640"
     CDS             9457..>9899
                     /locus_tag="PK171_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT04742.1"
                     /translation="MANVILKDIVKKFGDVLAVNKVNLEIKDQEFVVLVGPSGCGKTT
                     TLRMIAGLEEIDAGEIYIGDTLVNDVPPKDRDIAMVFQNYALYPHMDVYNNMAFGLKL
                     RKFPKSDIDQRVKDAAEILGIQELLQRKPKQLSGGQRQRVAVGRA"
BASE COUNT         3065 a         1963 c         1986 g         2885 t
ORIGIN      
        1 tcaccatcca taatttagcc tatcaaggca tcggaagtag tgaacttttt aaaattgtca
       61 atttacctgg tcactttttt actcatgatt acttagaatt ctacggtaac ttaaatcttt
      121 taaaagccgg tttggtcttc tccgatgtca tcaccactgt gagccctagt tatgctcgag
      181 aaatagctac gccagagggt ggcgagggat tagatgggct aataagagct attgggctaa
      241 gaaaaccgat catcggtatt gtcaatggaa ttgataccga tctttttaat ccagcaaccg
      301 acaaaaatct tccctatcct tatacaaacg gtcaattaga aataaaaaaa caaaataaac
      361 tggcttttta ccagcagtat ttcccggact tgaaagaaaa ggatgcctta ttccctttga
      421 tttcttttat ttctcgatta gttgaacaaa aggggttaga tctcattctg aacgatccag
      481 acaagttctt taatctcccc ctttgctggt ttttcctggg agttggagaa gccgcttatg
      541 aaaattcctt aaccgagtat gccaaaaaat atcccaacct tcatgtcgaa atggccttca
      601 acaatagttt agcccgattt gcttatgggg catcggatat gctggcaatg ccttctcgct
      661 ttgaaccttg tggaatcagt cagatgattg ctatgagata tggaaccatc ccagtagtaa
      721 gaaaaaccgg tggactcatt gataccgtga ttgattaccg atttaatcca gttttaaaca
      781 atgggtttca atttgataat tatcgtcccg atgaatttat ttttaccctc gagcgggcac
      841 taaactggta taatgatact cctgaacttt ggaaagtcat ggtcaaaaat gctatggatt
      901 ctgatttttc ctggaaaatc ccggttcagg aatatctcaa catttatttg ggttaataaa
      961 atttacgtat gattaaaata aaaagatcgg ttcctataat cggagttggc ggagcaaccg
     1021 gctcaggaaa atcgtcggta gcgcattcat ttagtactca taacgcaatg ataattgacc
     1081 tcgatcgttt atctcatgac ctctctcaaa agggaaggcg gttatggaaa gcagtggttc
     1141 ttgcttttgg ttcaggattt ttaaaccaaa agggagagtt gcagcgaaaa aagctgggaa
     1201 gagtgatttt taaaagctgg aggttgcttt ttctgctcaa tcgtattagt catcccattt
     1261 ttaaaaacga aataaaaaaa attattcttc accaacctcc ctcggctatg attgttatcg
     1321 atggtgctat attatttgag gccggactca ttcccttggt tgattgttta atttttgttg
     1381 aagcacccga ggagcaaaga tcagtccgtc tttcccaaaa aggattgtct cctgatgaca
     1441 tccgctctcg aatgaaaagt cagaaattta tttcctgttt acgacgaaga tctcatatta
     1501 tcatcgagaa tagtgctaca cctgaagacc ttcagcaaaa aataaatttt gtctgtcagc
     1561 ttcccattga aaaacttatc caatccgatc ggaaaaccgg gtgatattgt gccaaccaaa
     1621 ggacgctttc aattagtttc cgattatcag cctcagggag atcaaccaac ggctatagaa
     1681 aatctcaata aaggtatttc aaaccagtgc cggtttcaaa ctttattagg tgttaccgga
     1741 tcgggaaaaa ctttcactat ggccaatgta atcgaacatt accaacggcc aacccttatt
     1801 atctcaccaa acaagacatt agcagcacag ctttatagtg aaatgaaagc ttttttcccc
     1861 aataatgccg tagaatactt tgtgagttat tacgattatt atcaaccaga agcttacgtt
     1921 cctgcacagg atctttacat cgaaaaagat gcatctatta atgagcaaat cgatcggctt
     1981 cgattatcag ccaccagtgc tcttatggaa cggaatgatg tcgtggtagt cgcttcggtg
     2041 tcctgtattt acggcatcgg ttccccggag gaatatcgca agagaatctt ccctgtcagg
     2101 gtgaatgata cctggaatcg agatgaattt gcccatcgct tggttgactt gctttacgaa
     2161 aggaatgaaa tcgagtttat ttccggttgc tttcgaatga aaggggatac tatagaggtt
     2221 tatccagcct atagtgaatc agctctgcgt tttgaatttt gggggaacca agtcgaatgt
     2281 ataaaaattt ttgagccggt gagtggaaaa actttaaata aactgtcaga gattttcgtt
     2341 tatccggcta aacattatct tgtcgaccgg gaccaattcg atttagcctt gagaaatatc
     2401 gaactggaat tacaggatca gctcaagagt tttctttccc aaggaaaact ggttgaagct
     2461 gaccgtttag aaagaagaac ccgttatgac ttggaaatgc tgagagaaac cttctactgt
     2521 tcaggaattg aaaattattc ccgccactta gatggaagaa gacccgggga accaccttat
     2581 acacttatcg attattttcc tcgagatttt ctattattca ttgacgaatc tcatgttgcc
     2641 attcctcaaa tccacggaat gttagccggt gaccggtctc gaaaaaaagc cttggttgac
     2701 tttggctttc gtcttccttc ttgttttgac aatcgtccac tttcttttga agaatttgag
     2761 gcaaaattag gatacgttat ttatgtttcg gcaacgccag gaacttacga ataccaaaaa
     2821 agcgatcaaa ttgttgaaca gttaattcgt cctactggat tgattgatcc tgaaattata
     2881 gttcgtccgg caaccggcca agttgaggac ctttatcaag aaatcaaacc agtcattcaa
     2941 tccggagaga gagttttggt aacgacctta accaaaagga gtgctgaaga cctcagtgac
     3001 taccttttca atttgggaat caaagtgcga tatctccatt ccgaaattga aaccatcgaa
     3061 agagctcgaa tcattcgaga tttacgtgca ggagagttcg atgttttagt gggtatcaat
     3121 cttctacgag agggactgga tctacccgaa gttgctttag tagctgttct cgacgccgat
     3181 aaagaaggat ttcttcgatc ggaggtctca ttgattcaaa ccattgggag agcagctcgg
     3241 aacataaaag gtcgggcaat actctatgct gatcgactga ctggatcctt ggaaagagcg
     3301 atgcaggaaa ccgaaaggag aagaaaaacc caattagaat acaatcggac tcatcacatc
     3361 actccggaat cgataattaa agaagtacgg accttgatcc cggaaatggt tgggctctct
     3421 ccagaagccg aaaataccct ttaccagctc gatcagttag aaactgatcg agtagctgaa
     3481 gaagacctca ttgaaaagct tacccaggaa atgaaaaagg ctgcaaaaaa tcttgaattt
     3541 gaaaaagcag cgttgattcg tgatgagttg atacgaataa aaaaaaccgt tttccaaacc
     3601 gatcaaaaaa atatccgcaa tcaacaaaaa actgcctctc ggataagtgg taaatctcat
     3661 tcaaattgaa cgaaatcaac gaaaaagact aaactgaagc caaaaggtga ttgtattgat
     3721 aaattctttg ctgattaagg gtgcacgtga acataattta aaaaatatcg atattgaaat
     3781 cccccaccat aaactggtgg ttataactgg attgagtgga tcaggaaagt cctcactggc
     3841 gttcgatacc atattcgctg aaggacagcg tcgttattta gaatcccttt catcctatgc
     3901 tcggcaattt ttggaaaaaa tggaaaaacc ggatgtcgat ttaatcgaag gattatcgcc
     3961 agctattgcc attaatcaaa aggcttcctc tcacaatcca cggtcaacgg tcggtaccgt
     4021 aactgaaatc tacgattata tgagagttct tttcagtcgt tgtggaaaag tattttgtcc
     4081 tcagtgtgga aaacccatag cccgtcagac tatccagcaa atcgttgatg aaatcctttc
     4141 ttaccctgat ggaacccgca ttgtaattct tgctccattg gttcgagggc gaaaagcaga
     4201 atataaaaaa ctcctcaacg acatgctgaa aaatggattt gctcggtcct tggtagatgg
     4261 agaattaatt gaattggaag attgggattc cttgaatttg gatcgaaata aaaaacataa
     4321 cattgacctg gtggttgatc gactcaccac cagttccgaa gaacaacgcc gactcgccga
     4381 ttcaatcgaa attgcccttc gttatggaaa aggactggtt aaaattgggt tggtagctga
     4441 tggtatgcaa atggaagagc gaatatttag tgaacatttt gcctgtcccg attgcggaat
     4501 aagttttcct gaggtaaccc ctcgaatgtt ttcctttaac aatccttatg gagcctgtcc
     4561 cgattgtagt ggccttggaa ttttaaattt catcgacccc ggtttggtca tccctgactg
     4621 gtctaaatct ttagaccaag gagccataat cccctggaac agatatgacg aaaatcatta
     4681 ctccatgaac aaaattaagc gtttccttcg ggataaaggc attgctttta aagttccctt
     4741 tcaaaattta tccgaaaatg atcgacaatt aatcctttat ggagacgaag atggagatgg
     4801 aataatcagt tatttagaga agcgcatgaa ggctgccgaa agctggtggg aaatggatga
     4861 ggtagcccgt tttatggcga cacgaacttg cccgtcttgt cagggcaatc gacttcgtcc
     4921 cgaaagtctt tcggtaaaaa ttcaaaatca atcaattggc gatctttctc gatatactat
     4981 aggtgatttg gaaaacttct ttattacact aaaactctca aaacaagatg agcttattgc
     5041 tgccacttta ctaagagaaa ttcaagcccg gcttcacttt ttggtggaag ttggtttaag
     5101 ttatctcacc ctcagccgtt cggccatgac gctctccgga ggagaatctc agcgtattcg
     5161 gctggctacc caaattggtt caggtttggt tggggtactt tatgttctcg atgaaccaac
     5221 cattggtctt catccccgcg acaaccagaa gctgattcaa actttaaaaa gattacggga
     5281 cttgggaaat acagtcattg tcgttgaaca tgacgctgaa accatgaaaa attctgatta
     5341 tttaatcgat atggggccag gagctggaga aaagggggga gaagtaatcg caattggaac
     5401 cccagtggaa ataatgtgta accaaaagag tcttaccgga caatacctta gcgggaaaaa
     5461 ggaaatacca attcccttgg agagaaaaaa tccccaagaa tggttagtta ttcgtggtgc
     5521 tcgagccaat aatttaaaaa atattgatgt gaaaattcct ttaggggtca tggtgggact
     5581 cactggtgta tcgggttcgg gaaaaagcac tctcatggaa gacgtgcttt atcgatctct
     5641 ttctcggatt ttttatcatt cccttgccga accaggtgat cacgatacca ttgaaggtat
     5701 tgaatctatc gacaaggtga tcgttattga ccaatccccg attgggagaa ccccccgttc
     5761 caatcctgct acctataccg gaatttttac tgaaattcgg aatctctttg cccaactccc
     5821 cgaatccaaa gcccgtggtt atcgaccagg acgcttcagt tttaatgtga agggtggtcg
     5881 ttgtgaagct tgtcagggaa atggtgttat taaatttgag atgcattttc ttcccgatgt
     5941 ttttgtaacc tgtgaatatt gtaaaggaaa gcgatatgct cgagaaactt tggatgtaac
     6001 ctttaaggga aaaaatattg ccgaaatact cgatatgagt gttgatgaag cagcggtctt
     6061 ttttgaaaat attccagttg tacgccggaa attagctgtt cttcaacgag tcggacttgg
     6121 gtatatcaaa ctaggacaat cggcaaccac actctcaggt ggagaagctc agcgcattaa
     6181 gctaaccaaa gagctgagta agattgccac cggaagaact ctttatcttc tcgatgaacc
     6241 gactactggt cttcattttg ccgatgtcga aaaactactc gacgttcttt tggaattacg
     6301 ccgacgggga aatagtatct tgatcatcga gcacaatatg gatgtcatta aatgttgcga
     6361 ttatttggtc gatttgggac cagaaggtgg agaccaggga ggtcagctag ttgctgaagg
     6421 aactccagaa gaagtcgcct ccaatcctgc ttcgtatact ggtttttacc ttagggaggt
     6481 attaaagatt gagcgaaaag aattttgtca agtctgaaac cactcgaaat tcccttcagg
     6541 aattaccaac cagctgtggt gtatatattc tctgtaatca aaatcaccaa attatttata
     6601 ttgggaaagc cattaatatc caaaaccggg ttcgaaatca tttacagggt gactctgcat
     6661 ctcctctcaa gcgagacatg gctgaagaaa ttaccactat tcagttcatc ctcaccagag
     6721 atgaagccga agctcttctt cttgaagaag agctgattaa aacctaccaa cctcactata
     6781 acatccggat gaaagacgat aaaagttatc cctatattca tttcgatgct cgaggtgatt
     6841 ttccagcggt taaaatagcc cgacgcaaaa cggcaaaacc aggatatttt tatggaccgt
     6901 ataccaattc aaaaaaagct cgtgatgggc tgaaaattct ccgacaaatc tttcttcttc
     6961 gcggttgttc aattcctgaa tctcgttttc ctttgaaacg ctcttgtctt gactacgaat
     7021 tgaaattatg ttgtgctcct tgcgtttgga aaggcagccc ttcagagtat caagataaag
     7081 taaaaaaagc tcggtcattt ttagaaggag attatttgga ggttaccaac tggttagagg
     7141 aagaaatgtg gaaagccgcc gatcggcagg attttgaaaa ggcgattcaa ttccgagatc
     7201 gtctcgactg tctccttaaa attattgggc gataccggct agtccttcct gacagttctg
     7261 atgttgactt tattgaattg gaaaatgaat ccaatctggc cagtttagtt gtcgttaagg
     7321 tgagaaaagg aaggttgatt ggaatcgaaa atttccatgc tcaaggagag gaaaacgctg
     7381 atcggaacta tatattaaat gaatttattg aaaattttta tcttgatcac ttcaacccac
     7441 caacaaaaat ttgtgtccaa tttgattata atcgtaatct ccaggaaaga attaaagaaa
     7501 agggttactc aagcatcatt gaattacctc gcaatacatc cgagctcgat cttctccatt
     7561 ttgcccaaga aaacgcccaa aaaaatcttc aattagaaaa aattaaagcc cttcgccaaa
     7621 atctggatca tcaacaccta ctccatgaat taaagaacct tctttcactt cctgccttac
     7681 ctcgtctgat tgaaggagta gatatttcaa cctttcaggg tgatgaatcg gtaggggctg
     7741 tcgttgcttt tttcgatggc ttaccactaa agaaaaggta tcggaaatat atcatccgag
     7801 aagccgatca tcccgatgat tttgctatga ttgaagaaac tcttacccga catttcaata
     7861 aattaattca gaaccaaacc gagttaccca accttttggt tattgatggg ggtatcggcc
     7921 aattgaatag cgcactcaaa gttttacaga aattttcctt aactattcct gttctttctt
     7981 tagcaaaaga aaatgaagaa attttctttc ctggaaaaag tatttcgctc cgtcttccac
     8041 ccgaaaattc ttctctccag ttcctccaaa gaattcgcaa tgaagtgcat cgatttgtta
     8101 tcacctttca tcgggtgaga agaaataaaa aaatgttttc ctcccttttg gacgatatac
     8161 cgggaattgg tcctcaacgg aaacaggtcg tcctttcagc ttttgacaat ctgacccaaa
     8221 ttttaaatta ttctccccaa gaattatccc gtcaactaaa aataccctcc caaagtatta
     8281 ttaaaatcca aaaaaaaatc agccaaaaaa atatgcttgc cggaaaagag ggaatcactt
     8341 cttcatgaac tttcaagaaa gcttaaaaac cgagctattt accattcaac cgcagaataa
     8401 agatgaagtc atgagtgaat ttattggatt gattagaact ggtgcagtga taaaaaaaca
     8461 ttcccacgag cttcttttac ttttctccag ccgccatcca gctttgatta aaaaatgtgt
     8521 cacccataaa aaaagccttt tccctcattc ttcccataca attagtgtcc aagagagaag
     8581 gggaattcca ggaggtatat tttactgttt cgattttagc gttaccaata tcatgctcga
     8641 tcaatttggt tttcatgatg ttaacatcct aagcaactat ttaaatcaga agttagcagc
     8701 acatttttta agaggagttt ttgaatcacg gggatatata agcaatccca tgaggggata
     8761 tcacttggaa atacagttca attcggaaaa acttgctcat tacattctaa aatatcttca
     8821 caatctccag tttgattttc gctcccgtac ggttaaagga gatattaatc tctatttaaa
     8881 aaattctcaa ttaatagctg attttattcg tttcctggga gcccatcaaa gttacctcga
     8941 aatggaaaaa attatggtag aaaaatcaac aattaatgaa attacccgct gggttaatta
     9001 cgaaactgct aatttaaaca aaattgtcgc atcttcatcg cggcaaagaa aaaagataca
     9061 agttcttgat tttgatgact tacctttcca tcttcaagaa attgccagat tacgtttaga
     9121 aaatccttgt tcttcattac gtgaaattgg tgacctttgt aatccacctc tttccaaatc
     9181 agaggttcac cgccgattaa ataaaattga aaaaattgcc gaccaaaaag ggtttacaga
     9241 aaaccttact tgacataaaa tatctatagt ggttagatta atattttaac actataaatt
     9301 ttattacgat aaaatatagt agtgataaga ttcctatttt acgagaaatt tggttttttt
     9361 gaaatccaac gtaattcccg ttgggtttca tgtcggcctt tttcgaaatc tggtttaaaa
     9421 aggcctcccc tattaagatt tgaaggagga atatagatgg caaatgttat tttaaaagat
     9481 attgtaaaaa agtttggaga tgttcttgca gttaacaaag ttaatcttga aatcaaagat
     9541 caggaatttg ttgtattggt tggtccttct ggttgcggga aaacaaccac gctccgtatg
     9601 atcgccggtt tggaagaaat cgatgctgga gaaatttaca ttggagatac cttggtgaac
     9661 gatgtccctc ccaaagatcg tgacatcgca atggtattcc aaaactatgc cttatatcct
     9721 cacatggatg tttacaacaa catggctttc ggcttaaagc ttcgcaaatt tccaaaaagt
     9781 gacatcgacc aacgagtcaa agacgcagcc gaaattcttg ggattcaaga gctcctccag
     9841 agaaaaccaa aacaactctc cggtggtcaa agacagcgtg ttgctgttgg tcgagctat
//
LOCUS       DAOSOL010000012         9797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6914_length_9797_cov_4.365839, whole genome shotgun sequence.
ACCESSION   DAOSOL010000012 DAOSOL010000000
VERSION     DAOSOL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6914_length_9797_cov_4.365839"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PK171_00645"
     CDS             complement(<1..484)
                     /locus_tag="PK171_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOT04743.1"
                     /translation="MYQRKWQIRKVNRGCLKHLASSLCSPISIARVLMNRGIITPHEA
                     RAFLDPDISSLDNLRSLPNLRKAVLLIDSVIKGNGKILIFGDYDADGLTACAILFLFL
                     KKLTPYVDTYIPSRFHEGYGLSEKAIENILNISPDLVITVDAGIKDVKGVGILKNKGI
                     R"
     gene            complement(490..1365)
                     /gene="secF"
                     /locus_tag="PK171_00650"
     CDS             complement(490..1365)
                     /gene="secF"
                     /locus_tag="PK171_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOT04744.1"
                     /translation="MEIFLKPNFNFVDHRKIPYAICIIFIILTVIGLVRGINYGVDFQ
                     GGTLLQYNFTQVVAAEEIRNVLDEFDLSASTIQRFSDNEVAIRTPQLTLDQRNQLTDT
                     LRNQIGELEIVRNEEVGPTVGGELRRAAYLALLFSIIGILIYVGIRFELRPAVTSVLA
                     LANTGIMTLGLVALLGIEFDTTILAAMLTILGYSINDSIVVMDRIRERLNYRKREDAK
                     TLINTSLNETLSRTIMTGTTTIIAVIILYFFGGKMLQPFALTMLIGLIFGTFSSIFVA
                     AMLFYEWEVKKPLRR"
     gene            complement(1384..2577)
                     /gene="secD"
                     /locus_tag="PK171_00655"
     CDS             complement(1384..2577)
                     /gene="secD"
                     /locus_tag="PK171_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOT04745.1"
                     /translation="MTKNWRDFPWRLIITIALVVLAIIAIFPIEKKIRLGLDLKGGSH
                     ILLECVDSPEAPLDEDSVKRVVEIVRNRIDQLGVAEPLILRQGEKRVLVQLPGIADPQ
                     RAVEIIGQTALLEFKDESGKTLLTGATLKNAQVQYDRFGRPTVSISFNEEGAKLFGQA
                     TTANVGKPIGIYLDGNIISNPVVQEPILQGDAQITGQFTLDEAQNLAILLRAGALPVK
                     VVVAEERSVGPSLGKDSITSGLRAAIIAGIFVFIFMFLYYGWLGILADIALICYALFT
                     LALLSVLQATLTLPGIAGFILSVAMAVDANVLIFERIKEEYRAGKTWRAAIQAGFDKA
                     FRTILDSNITTIIAAALLFFIGSGSVRGFGITLTLGIICSMFTGIWVTRVLIDHLAPK
                     PTIKS"
     gene            complement(2614..2949)
                     /gene="yajC"
                     /locus_tag="PK171_00660"
     CDS             complement(2614..2949)
                     /gene="yajC"
                     /locus_tag="PK171_00660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT04746.1"
                     /translation="MSFFTSIAYAATGGAATGGAAPATPPAGSMFTQLLPLIFIIVIF
                     YFLLIRPQQQKQKAQRDLISSIQKGDHVVTIGGIHGTVVQVSDDEITLEVSKGVNIRF
                     VKSAVSTKK"
     gene            complement(2983..4101)
                     /gene="tgt"
                     /locus_tag="PK171_00665"
     CDS             complement(2983..4101)
                     /gene="tgt"
                     /locus_tag="PK171_00665"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT04747.1"
                     /translation="MKSFTLVHQDEQSKARVGILHTSHSDIPTPVFMPVGTQGTVKAM
                     APDRLKAVGVKMLLGNSYHLHLRPGEDLIREMGGLHRFMSWNGSILTDSGGFQIFSLS
                     DLVKIDDQGALFRSHLDGSLHHFTPEISMQIQQDIGADVVMILDQCIGYPASDYQVKV
                     GTDRTLVWGKKCREAFQSESQMLFGIVQGGTLPELRRYSAEETVKIGFDGYAIGGLSV
                     GEPKPIMYSMIDEVEPYLPKSQARYLMGVGSPDSIIEGVRRGIDMFDCVLPTRNARNS
                     ALLTWSGRMNIDRLEYSRDSNPIDPQCQCYTCKNFSRSYLRHLFKAGEILAAELATIH
                     NLFFMVELLNRIRKAILNGSFRDLSSDLFARFQGQIVN"
     gene            complement(4180..5208)
                     /gene="queA"
                     /locus_tag="PK171_00670"
     CDS             complement(4180..5208)
                     /gene="queA"
                     /locus_tag="PK171_00670"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT04748.1"
                     /translation="MILTDQFNFHLPDELIAQRPVVPRDQCRLMVINRLSQEIEHRRF
                     FDLGHYLKPKDLIVINDSRVIPARFYTQKNPTGGKVEILFLHRHQELWACMLNHSRRV
                     KEGTILSLPEHPEIFWQVEGREKDWWLLRPSFPPEKEDEIFFNYGTTPTPPYIKTPNV
                     KLEEYQTIYAQRKGSVAAPTAGLHFTQSLIDQLKNLGIQFASLTLHVGLGTFFPVKTS
                     TIEEHHMHSEWYEVDQKTAEMIQETKKNEGRVIAVGTTVVRVLESIYRQRGRIVEQSG
                     ETDIFIYPGFQFQVINAMITNFHIPRSTLFMLVCAFAGTEFMKAAYRGAVEKHYRFFS
                     FGDATFIM"
     gene            complement(5205..6641)
                     /locus_tag="PK171_00675"
     CDS             complement(5205..6641)
                     /locus_tag="PK171_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT04749.1"
                     /translation="MMDKKKIVIPLMFLFFLFFFFGEAISAQNDFIVTVCIVNGKSDV
                     ILSSTAGLKVQIDKKVYSITAQEQLSIIASKTKVFWEERQIVSSSFRVTTQGKGFILV
                     NNRPYRGSLTLKNIQGHLIVINHLYLDDYIRGTIKMEINPHWPEEAVKAQIVAARTYA
                     VKNLSRHHDQGCDFCASSHCQVYGGVNAEDPITNKLIDSIKGLILTYQNQPAGVCFHS
                     ESGGYTDSALNVWGRSVPYLVSVESPWESDSPHAQWELEISSEELARSLQRAGFIQGS
                     IVDVQVQPNSGSHRVREFLIQTTLKRYRIPASKVREALGYDRLPSTYFELTPINKTML
                     PSRPTPKPKATPLPSTPISGNPGSDDKDPGYREMLDKDWNLDDILTFLSLREQERQKS
                     NSRKINQPSPSNPVEEFEEKEALEIPDNPQTTYRTHSVYLFKGKGYGHGVGLSQWGAR
                     GMAMKGYSYQDILFHYFPGCEIKKARFK"
     gene            complement(6645..6872)
                     /locus_tag="PK171_00680"
     CDS             complement(6645..6872)
                     /locus_tag="PK171_00680"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOT04750.1"
                     /translation="MEISLWAKTIIIIGVVLIIIGLVMVFLPKIPILQRLGRLPGDIV
                     VKKGNVTFYFPWVTCIVISIILTLIFSIFRR"
     gene            complement(6839..7408)
                     /locus_tag="PK171_00685"
     CDS             complement(6839..7408)
                     /locus_tag="PK171_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOT04751.1"
                     /translation="MSKPQLLLHTCCGPCAAGIQAELEKEGWEVTFLFYNPNIHPYDE
                     YCRRMESFYRYLEMTGQKGLTPFPYQPADYFQSISSEINRCSGCYRLRLKKVAEWGKR
                     NSFQHFATTLTVSPYQNQEKIFLVGEQIAHNNDMVFLKRNFQPLYRNSRDRSVELNLY
                     QQKYCGCIFSLWEGDKKRLWKYLFGQKPL"
     gene            complement(7405..8409)
                     /gene="ruvB"
                     /locus_tag="PK171_00690"
     CDS             complement(7405..8409)
                     /gene="ruvB"
                     /locus_tag="PK171_00690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOT04752.1"
                     /translation="MNDAPKQKDFIPFLRDTDDDISLRPKRLNEFIGQDKVRANLNVF
                     IQASLARDESLDHVILYGPPGLGKTTLASIIANEMNAAIRYLSGPSLTRSGDVASILT
                     TVTDRDILFIDEVHRLPRVCEEILYSAMEDFALDILIGKGPGARSVRISLPPFTLIGA
                     TTRISLLSAPLRNRFGIIEKLDFYNQGDLSAIVLRSSAVMNIPIDQDASEEVARRSRG
                     TPRIANRILKRVRDFAQYYGKSAIDKELVRDALECLEIDDMGLNRMDRNLLKILLEHY
                     HGGPVGINNLAMMLGEDAATVEEVYEPFLIKVGLLIRTPRGRMITPQGHQYIKGKKLL
                     "
     gene            complement(8406..9002)
                     /locus_tag="PK171_00695"
     CDS             complement(8406..9002)
                     /locus_tag="PK171_00695"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HOT04753.1"
                     /translation="MFDSIRGTVAAIENDEWILETDWYGFRIKVTPFISSGKIEEQKK
                     LFIRFYFREDGEFFYYGFEDPKERETFDLICGVKGIGHKTAFKILSRIHWKEFTDLII
                     AENVDYLSSRINLSSKTVKRLLLELKPRLDKVGFVTTDAPKVTKTWKEVKTALSGLGY
                     TAGEVEGVLNILWQEDQALFDDTEILLKKALGKIGGLK"
     gene            complement(8995..9507)
                     /gene="ruvC"
                     /locus_tag="PK171_00700"
     CDS             complement(8995..9507)
                     /gene="ruvC"
                     /locus_tag="PK171_00700"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT04754.1"
                     /translation="MSSQEKIVLGIDPGTATTGYGIIAKRKGSGVVKAVKYGVIVTKK
                     ELPLSERLFIIYQEISQLINNYHPAEVAVEEIFFNKNSKTAISVGEARGVILLNAAMQ
                     SIPVFEYTPLQVKQSVVGYGRAKKEQVIYMVKTILNIQEVKLTPDDVADALAVAICHA
                     FQSEINYSYV"
     gene            complement(9517..>9797)
                     /locus_tag="PK171_00705"
     CDS             complement(9517..>9797)
                     /locus_tag="PK171_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT04755.1"
                     /translation="IDAGAEDIQTFDTTIDIVTSPGDFDRIKALIDDNKMVYSNAEIT
                     MVPKTTVHLEGKQAQQTLNLIDSLEELDDVQEVFSNFEISDELLESLE"
BASE COUNT         2985 a         2091 c         1810 g         2911 t
ORIGIN      
        1 ctcgtattcc tttatttttt aaaataccga ctcccttgac gtctttaata ccagcgtcca
       61 cagtaataac taaatctgga ctaatattga gtatgttttc aatggccttt tccgataaac
      121 catatccttc atgaaaacga ctggggatat aggtatccac gtaaggggtt aattttttca
      181 aaaaaagaaa gagaattgca caagcagtta atccatcggc atcataatcc ccaaaaataa
      241 ggattttccc attccccttg ataactgaat caattaataa aacagctttt ctgaggttgg
      301 gtagagaacg gagattatcc agagaagata tatctggatc taaaaaagct cgagcttcat
      361 gaggggtaat aataccccga ttcataagca ctcgcgcgat cgagatcggt gaacagagac
      421 tgcttgctaa gtgcttcaaa cagcctctgt tcacttttcg aatttgccat ttcctctgat
      481 acaaatagtt tacctcctca gaggtttctt gacttcccac tcataaaaga gcattgcagc
      541 cacaaatatt gaagaaaaag tcccaaaaat taaacctata agcatggtga gagcaaaagg
      601 ttgcagcatc tttcctccaa agaaatagag gattataact gcaataattg ttgtcgttcc
      661 agtcataatg gttctagaaa gagtttcatt gagactggta ttgataaggg ttttagcatc
      721 ttcgcgctta cggtagttta atcgttcgcg aatcctatcc atgaccacga tagagtcatt
      781 aatcgaatat cctaaaattg taagcatcgc tgctaatata gtagtgtcaa actctatacc
      841 taataacgct actaacccta aagtcattat accagtattg gctaaagcca aaactgaggt
      901 tacggctggt cgtaactcaa aacgtatccc aacataaatc aaaattccta taatagaaaa
      961 taggagagcc aaataggcag cacgtctcaa ctcgcctcct actgttggtc ctacttcttc
     1021 atttcgaaca atttccaact caccaatttg atttcttaag gtatcggtga gctgatttct
     1081 ctggtctagg gtcaactggg gtgtccggat ggcaacttca ttgtccgaaa atctttggat
     1141 agtgctggcg gaaagatcaa attcgtctaa tacatttcga atttcttcgg ctgctacaac
     1201 ttgagtaaaa ttatactgaa gaagcgttcc gccctgaaaa tccactccat aatttatacc
     1261 acggactaac ccaatcacag tgagtatgat aaatataata caaattgcat acggtatttt
     1321 tcgatgatca acaaaattaa aatttggctt caaaaatatt tccatatcgg gtccctccgc
     1381 atcctatgat ttaattgttg gctttggtgc gagatgatca attagcactc gtgtcaccca
     1441 gattccggta aacatactac agatgatacc taaggtgagc gtaataccaa acccacgcac
     1501 cgaacccgat ccaatgaaga acaacaaggc tgcagcaata atagtggtta tgtttgagtc
     1561 aagaatggtt cggaatgctt tatcgaaacc agcttgaatt gctgctctcc aagttttacc
     1621 agcacgatat tcttctttaa ttctttcaaa aatcagaaca ttggcgtcaa cggccatagc
     1681 aacagataga ataaatcctg caatacctgg tagagtaaga gtggcttgca agactgaaag
     1741 taaggctaaa gtaaacagcg catagcatat taaagcaatg tcagcaagaa ttcccaacca
     1801 cccgtaatag aggaacataa agataaatac aaatataccg gcgattatcg ctgcccttaa
     1861 tccactggta attgaatctt ttccaaggga tggaccaacc gaacgctctt cggcaacgac
     1921 cactttcact ggaagggcac cagctcgtaa cagtatagct aaattctgag cttcatccag
     1981 agtaaactga ccagtaattt gagcatctcc ttggaggata ggctcctgaa ctacaggatt
     2041 agatattatg tttccatcaa gataaatgcc gattggtttt ccaacattgg cggtagtagc
     2101 ttggccaaat agttttgctc cttcttcatt aaaagaaatg gagacggttg gtctgccaaa
     2161 acggtcatat tgaacctgag catttttaag tgtcgcaccg gttaataagg tttttcccga
     2221 ctcgtcttta aattccaata aagcagtttg cccaataatc tccactgctc tttggggatc
     2281 ggcaattcct ggaagctgga ctaaaaccct tttttcaccc tgtcggagaa ttaacggctc
     2341 agctactcct aattggtcga tacgattacg gacaatttca accactcgtt tcactgaatc
     2401 ttcatcgaga ggagcttctg gagaatccac acattctaaa agaatatggg aacctccttt
     2461 taagtccaat cctaaacgaa tttttttctc gataggaaat attgcaatta tagccaaaac
     2521 gactaaagcg attgtaatga ttaatctcca gggaaaatct ctccaattct tagtcaaaac
     2581 atcctgcccc ctttacccaa tttcttttcc tctttatttt ttggtcgaaa ccgctgattt
     2641 gacaaaacgg atattgactc ccttggaaac ttcgagggtg atttcatcat cagatacctg
     2701 aacgacagta ccatgaatcc caccaatagt aaccacatga tcacccttct gtatactact
     2761 gatcaaatcc ctctgggctt tttgtttttg ttgttgtggt cgaatgagca aaaagtaaaa
     2821 tatgacaatg atgaaaatga gcgggagcag ttgcgtaaac atgctaccgg ctggcggggt
     2881 tgccggcgct gcaccaccgg ttgctgcacc accagtcgct gcataagcta ttgatgtaaa
     2941 gaaactcata ttcatatcac tcctttaaaa aagtttgttt tattaattta caatctgtcc
     3001 ctggaaccga gcgaagagat cagacgataa atctcgaaag gaaccgttta aaatggcctt
     3061 acgaatacga ttcaataact ctaccataaa aaaaagattg tgtatagtag ccaattctgc
     3121 tgctaaaatt tccccggctt taaacaaatg acgcaaataa gagcgagaaa aatttttaca
     3181 agtataacat tgacactggg gatcaatggg attggaatct cgagaatatt ccagccggtc
     3241 gatattcatt cttcctgacc aggttaataa agcagaattt ctcgcattcc tggtaggaag
     3301 aacacaatcg aacatatcga ttcctctcct gactccttca atgatactat cgggtgatcc
     3361 tactcccata aggtatcgag cttgagattt gggtaagtaa ggttctactt catcaatcat
     3421 tgaatacata atcggtttgg gctcaccgac acttaatccc ccaatggcat agccatcaaa
     3481 gccaattttt accgtttctt cagcgctata ccttcttaac tcgggaagtg tccctccttg
     3541 tacaatccca aacaacattt gcgattccga ttgaaaggct tcccggcatt ttttgcccca
     3601 caccaaagtc cgatcggttc caaccttgac ttgatagtca cttgccgggt atccaataca
     3661 ttgatcaaga atcataacca catcggcacc aatatcttgc tgaatttgca tcgatatttc
     3721 aggagtaaaa tggtgaagag acccatctaa atgagagcga aaaagagctc cttgatcatc
     3781 aatttttact aaatcagaaa gactaaagat ttggaaacca ccgctgtcgg tgagtattga
     3841 accattccaa gacataaaac gatgaagacc gcccatctcg cgtattaaat cttctcctgg
     3901 ccgaagatgg agatgataag aatttcccag taacattttc actcccacgg cttttaaacg
     3961 atcaggagcc atggctttca cagttccctg agtcccaacc ggcataaaaa ctggggttgg
     4021 gatatcacta tgagaagtat gcagtattcc aacccgggct ttggattgct catcttgatg
     4081 cacgagtgta aaagatttca tgagtattga tttctccttt attcaaaaac agtaatcggc
     4141 tgatggattt tataatatta aacttcataa attatacttt tacataatga aagttgcatc
     4201 tccaaaacta aaaaaacgat aatgcttttc aacggctcct cgataagcag ctttcataaa
     4261 ttctgttcca gcaaaagcac ataccaacat aaacaaagtg gaacgaggga tatgaaaatt
     4321 ggttatcatg gcattaatca cctgaaactg aaatcctgga tagataaaaa tatcagtttc
     4381 tccactttgt tcaactattc ttcctcgttg acgataaatg gattcaagaa ctcgaacaac
     4441 tgttgtacca actgcaatca ctcgaccctc attttttttt gtctcttgaa tcatttcagc
     4501 cgttttttga tctacctcgt accattcgct atgcatatga tgttcctcga tggttgaggt
     4561 tttaaccgga aaaaaagtcc ccagacccac atggagagtg agtgaagcaa attgaatgcc
     4621 caggtttttc agttgatcaa ttagcgactg agtaaaatgc agtccagcag tcggagccgc
     4681 taccgagcct ttccgttgtg cgtaaatagt ttggtattct tctaacttta cgtttggcgt
     4741 tttaatataa ggtggtgtcg gtgtggttcc ataattaaaa aatatttcat cttctttttc
     4801 aggtggaaaa gagggacgaa gaagccacca atctttttct cttccttcta cttgccaaaa
     4861 aatttctgga tgttccggaa gggagagaat ggttccttct ttaacccgcc gggaatgatt
     4921 gagcatacaa gcccagagct cttggtgccg gtgcagaaaa agaatttcaa ctttcccacc
     4981 agttggattc ttttgagtat aaaaccgagc tggaatcacc cgagaatcgt tgatgacaat
     5041 aaggtcttta ggtttcaaat aatggcccaa atcgaaaaac ctgcggtgtt caatttcttg
     5101 agagaggcga ttgatgacca ttaatcggca ttgatctcgc ggaacgaccg gtcgctgagc
     5161 gatgagttcg tcaggcaggt gaaaattaaa ttgatcagtt aaaatcattt aaaacgagcc
     5221 ttttttattt cacaaccagg aaaataatga aacaatatat cctggtagga atatcctttc
     5281 atagccattc cccgtgctcc ccattgggaa agaccaaccc catgtccata acctttccct
     5341 ttaaataagt aaacagaatg agtacgataa gtagtttggg gattatcggg aatttcaaga
     5401 gcttcctttt cttcaaattc ctccacgggg ttagaagggg aaggttggtt gatttttctt
     5461 gaatttgatt tctgcctttc ttgttctcga aggcttaaga aagtcaagat atcgtcaagg
     5521 ttccaatctt tatctaacat ttcccgatag cccggatctt tatcgtcgct acctgggtta
     5581 ccagaaattg gagttgaagg taacggagtc gctttgggtt ttggtgtcgg tctggacggt
     5641 aacatggttt tatttatggg ggttagctca aaataagtac taggaagtcg gtcgtaaccc
     5701 aaagcttcgc gaactttgct cgctggaatt cggtatctct tcagtgtagt ctgaattaaa
     5761 aattctctta ctcgatgact tccggagttt ggttgtactt gaacatcaac tatcgaaccc
     5821 tgaataaatc cagctctttg taaagatcga gccaattcct ccgagctgat ttccaactcc
     5881 cattgggcgt gaggagagtc gctttcccag ggagattcaa cgctcaccaa gtaaggaaca
     5941 cttctccccc aaacatttaa agccgaatcg gtatatcccc cgctctcgga atggaaacaa
     6001 actcctgctg gttgattctg gtaagtaagg attaaacctt ttatagaatc tatcagttta
     6061 ttggtaatag gatcttcagc atttactccc ccatatacct ggcagtgtga tgaagcacaa
     6121 aaatcgcaac cttgatcatg gtgacgactt aagtttttta ctgcataggt tcgggccgca
     6181 acaatctgag cttttacggc ttcttcaggc caatgtgggt ttatttccat cttaatcgtt
     6241 cctcgaatat aatcatctaa ataaagatga ttaatgacaa tcaaatgtcc ctggatattt
     6301 tttaaagtca aagaccctcg atagggacgg ttattcacca ggataaaacc ctttccctgg
     6361 gtcgttaccc ggaaagaaga agatactatt tgtctctctt cccaaaacac tttggtctta
     6421 ctggcaataa tagatagctg ctcctgggct gttatcgaat aaactttttt atcgatttga
     6481 acttttaatc cagcggtaga agaaagaata acatcagatt taccattgac aatacaaact
     6541 gtcacaataa aatcattttg ggccgaaata gcttcaccaa aaaagaaaaa taaaaaaaat
     6601 aaaaacatca agggaataac tattttcttt ttatccatca tgccttaccg cctaaatatt
     6661 gagaaaatga gtgttaatat gatactgata actatacagg tcacccaggg gaaataaaag
     6721 gtaacatttc cttttttcac aacaatgtca cctggaagtc ttcctaaacg ttgaagaatt
     6781 ggtatttttg gcagaaacac catcaccaaa ccgattataa tcaatacaac tccaataatt
     6841 ataatggttt ttgcccaaag agatatttcc ataaacgctt tttgtctcct tcccatagac
     6901 tgaaaatgca accacaatat ttttgttgat aaaggttcag ttcaaccgat cgatctcggc
     6961 tgtttcgata taagggttgg aagttccttt ttaaaaaaac catatcgtta ttatgagcta
     7021 tttgttcacc gaccagaaat attttttcct gattctgata agggctgacg gtgagagtgg
     7081 tagcaaaatg ctggaaagag ttccttttcc cccattccgc cacctttttt aatcgtaatc
     7141 ggtagcagcc actgcaacga ttgatttctg aggaaattga ttgaaaataa tctgccggtt
     7201 ggtaaggaaa gggagttagt cctttctgac cggtcatttc caaataacgg taaaaacttt
     7261 ccattctccg acaatattca tcataggggt ggatattggg attataaaaa agaaaggtga
     7321 cctcccatcc ctctttctca agttctgcct ggattcctgc tgcacatggc ccgcaacagg
     7381 tatggagaag aagctgcggt ttactcataa tagctttttc cctttaatat attgatggcc
     7441 ttgaggagta atcatccttc ctcgaggggt acgaatcaat aatccaacct taattaagaa
     7501 aggttcatag acctcttcga ctgtagcggc atcttcacct aacatcattg ctaagttgtt
     7561 aatacccacc ggccctccat gatagtgttc caataaaatc ttcaataaat ttcgatccat
     7621 gcggttaaga cccatatcat caatttcaag acattctaag gcgtctctta ccaactcttt
     7681 atcaattgct gattttccgt agtattgagc aaaatcccta actctcttta aaattcggtt
     7741 ggctattcgt ggtgtcccac gagatcttcg agctacttct tctgaggcat cttgatcaat
     7801 cggaatattc ataactgctg atgaacgaag aacaatagcc gataaatcac cttgattgta
     7861 aaaatcgagt ttttcaatga taccaaaccg atttcgtaag ggcgcactta aaaggctgat
     7921 gcgggtcgtt gctccaatga gtgtgaatgg aggtaaacta attcttacgc tccttgcacc
     7981 tggtcctttt ccgattaaaa tatccagagc aaaatcctcc atggcactgt agagaatctc
     8041 ctcacaaact ctggggagtc gatggacttc atcgataaat aaaatatctc gatctgttac
     8101 tgtagttaga attgaggcaa catctcctga gcgggttaaa ctaggaccgg ataaatagcg
     8161 aatcgcggca ttcatttcgt tggctataat tgatgctaaa gtcgtttttc ctaaacctgg
     8221 aggtccataa agaataacgt ggtctaaaga ctcatctcga gctaatgaag cttgaataaa
     8281 aacgttgaga ttcgccctta ctttatcctg accgataaac tcgttcagtc ttttgggtcg
     8341 taatgatata tcatcatcgg tatcccttaa aaaagggata aaatcttttt gctttggagc
     8401 atcgttcatt tcaaaccacc tattttaccc aatgcctttt ttaaaagaat ctcagtatcg
     8461 tcaaatagag cctggtcctc ttgccaaaga atatttaaaa caccctcaac ttcaccagca
     8521 gtatatccca aaccagaaag agctgtttta acttctttcc aggttttagt taccttcggc
     8581 gcatcagtgg tgacaaagcc aactttatcc aaacgaggct ttaattctaa tagaagcctt
     8641 ttaactgttt tcgaagagag attaattcga gaagaaagat agtcaacgtt ttcagcgatt
     8701 ataaggtcgg taaattcttt ccagtgaatt cgggaaagaa ttttaaaggc agttttatgg
     8761 ccaatacctt tgactccaca aattagatcg aaggtttctc tttctttagg atcttcaaat
     8821 ccatagtaaa aaaactcacc gtcttcgcga aaataaaaac gaataaacag ttttttttgt
     8881 tcttcaattt ttcccgaaga aataaagggg gtcactttaa tccgaaagcc ataccagtcg
     8941 gtttcaagta tccattcatc attttcaatt gctgcaaccg ttcctcgaat tgaatcaaac
     9001 ataggaataa ttgatttcag actggaaagc atgacaaata gctactgcta aagcatcagc
     9061 gacatcatcg ggagtcaatt ttacctcttg tatattaagt atcgttttca ccatatagat
     9121 tacctgctcc ttttttgctc tcccatatcc gaccaccgat tgcttgacct gaaggggagt
     9181 atattcaaaa actggaatag attgcatggc agcattcaac aagatcactc cccgggcctc
     9241 accaacagaa atagcggtct tggaattttt attaaaaaaa atctcctcaa ccgctacctc
     9301 ggcaggatga taattattta ttaattggct aatttcctgg taaattataa ataatctttc
     9361 cgagagtgga agctcttttt tggtaacaat tacaccgtat ttaacagctt taactactcc
     9421 gcttcctttt ctttttgcta taattccata tccggttgtt gctgtacccg gatcaattcc
     9481 taagactatt ttttcctgag atgacatcag gagttactac tccaacgact ctagtaactc
     9541 atcagaaatc tcaaaattgg aaaatacttc ttgtacatca tccaattctt ctaagctgtc
     9601 tataaggtta agagtctgtt gggcttgttt gccttccaaa tgaacagtag tttttggcac
     9661 catggtaatt tcagcattgg aatagaccat cttattatca tcaattaaag ctttaatccg
     9721 atcaaaatct cccggcgaag taacaatatc aatggtcgta tcgaaggttt gtatgtcctc
     9781 cgcaccagca tctatag
//
LOCUS       DAOSOL010000013         9789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_6925_length_9789_cov_4.064722, whole genome shotgun sequence.
ACCESSION   DAOSOL010000013 DAOSOL010000000
VERSION     DAOSOL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9789
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6925_length_9789_cov_4.064722"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /gene="infC"
                     /locus_tag="PK171_00710"
     CDS             <1..230
                     /gene="infC"
                     /locus_tag="PK171_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT04756.1"
                     /translation="DDHDYQVKLKSAIRFLEDGDRVKFTIRFRVRESAYVDKGVLLLQ
                     KIIEDLNDLSKIEQEIKNEGRNLTMTIAPKK"
     gene            243..440
                     /gene="rpmI"
                     /locus_tag="PK171_00715"
     CDS             243..440
                     /gene="rpmI"
                     /locus_tag="PK171_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOT04757.1"
                     /translation="MPKMKTHRGLAKRVKITAKGKIKVAQGGKSHHLHQKEQDRKRRL
                     RKTVIVDKSIEKRIKKLLPYA"
     gene            543..896
                     /gene="rplT"
                     /locus_tag="PK171_00720"
     CDS             543..896
                     /gene="rplT"
                     /locus_tag="PK171_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOT04758.1"
                     /translation="MPRVKNSVASRQRKKKIMKLASGYRGSRSRSYRRANEQVLKSQF
                     YAYRDRKNKKRDFRRLWIVRINAAVREHGLKYNQFIFALKKAGIEIDRKNLSNIAITD
                     PVAFAHLVEVAKSAT"
     gene            1019..2038
                     /gene="pheS"
                     /locus_tag="PK171_00725"
     CDS             1019..2038
                     /gene="pheS"
                     /locus_tag="PK171_00725"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOT04759.1"
                     /translation="MLINLNNVLEKFQNEIEQTKTLDDLNRIKGKYIGRKGQLNELFK
                     EITQLSSDEKKDWGKKINHLKGLIDNQLQEKLKELTQTLKTSIKVDISLPGKKPWVGS
                     FHPIQLTLHQILTIFQSLGFRIESGPEIESEYYNFEALNFPPDHPARDAQDSFFLDKE
                     YLLRTHTSPSQIRIMQKIQPPFKVVVPGKCFRRDAMDASHSPMFHQVEGLVVAETISM
                     GDLKGTIEVFAQRFFGNDRQLRFRPSFFPFTEPSAEVDISCGLCQGKGCRSCGQSGWL
                     EIMGAGLVHPKVFEAAGYNPNQVRGFAFGMGIDRAALLKFGIPDIRWLFENDMSLIEQ
                     FHAIQ"
     gene            2054..4441
                     /gene="pheT"
                     /locus_tag="PK171_00730"
     CDS             2054..4441
                     /gene="pheT"
                     /locus_tag="PK171_00730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT04760.1"
                     /translation="MKVAYSILKRFVPNLDCPAEKIAEILTSQGFVIESITSATDTFS
                     PNLTAATIVKRKSGILYDVCTVQIKDRQYDVKCEKWGIPDIGKKVIVNPIGDLISTQP
                     LRNQRIPEKEEFFIEIPATIEAEEGELISPQIIGEDWVFDVEITANRGDCMSIIGLAR
                     EIAAGLDLEYQIPSQHLDVDDSISNFQVNIDDQDLCPFYSGRLAKNFKIQPSPWWIVS
                     ELYLLGQRPINQVVDVTNLVMMETGQPLHAFNASGIFDQKIVVRRAKNNESLITLDGI
                     ERKLTNNMLVIADANRPVAIAGIMGGYESEVSPDTQEVFLEAAIFNGSQIRKTARELG
                     LRTEASARFERGVDPGSVFYASERALYLLKEIDPSIKILKDWVVSGEVVSSEPEIEFS
                     SSWVEEIMNCKIPLEKMQTVLTKLGFVLLSQPNNSIIKVKVPSWRPDVQQPIDCAEEV
                     GRIFGYDHIRSQIPSFPFDPGNPRRELLWEGVLRNHLRSIGLKECVSLSLTSQANCDL
                     LGIPEADVIRVLNPLSQDHVILRPDLIISALDIIKTNIARGRENFGFFEYGEVFSRTN
                     DLDNPYCEEWRLVVVLSGHSYPAYWQQFSQIDIFSLKGFAESLIELIGYPLQMVNWQP
                     LDENPYFDSSLSFRGILKNGIEIFRGGLICKTISDAFGFWGEHYCLEVAWQKLFDGIK
                     LNDFQLQEINRYPSIRRDISIVVNQDQTWKNIESLVLKVASSHDLPMERVELFDSYQG
                     KHLPPGKKTFSYSIVFRSTHKTLTDNEVDEWVNIIKAAIKKEPELFLREELAGGF"
     gene            4465..5007
                     /locus_tag="PK171_00735"
     CDS             4465..5007
                     /locus_tag="PK171_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOT04761.1"
                     /translation="MFILILNVIRSSIRGFSKEILALTGVVIGLLSALRFHQNLGQFL
                     TNLFHCNSVWINLISFFVIFIPVVLLFSWVGMFFRKVFEGLDIVWFDAILGFVIGILK
                     GFLWISIITLFVLNVSFLEFLNNGIYQSRFYQSFTQPIIVFIDSMVQKIPEFSFLNAY
                     LEKGLNQSDDELIQRYTEEF"
     gene            4973..7345
                     /locus_tag="PK171_00740"
     CDS             4973..7345
                     /locus_tag="PK171_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOT04762.1"
                     /translation="MNLSKDILKNFDFDKILHQLSSACVCDVSREKMMELQPYTNPEI
                     INQRLQTVQQLLDFITFDGSLDLSGLTDLREIFQKIAIPGSALNLNQVIQVFHFLLLC
                     EKTKHLQNETRIKEKYPLIKVFFDAIESYAPLIKEIKLICSPDGLILDTASPRLRDIR
                     RRLNRLQVEIRTTIENCLRNRDIAPYLQDTTYTIRRGRYVIPIKSEFRGRIDGIVADY
                     SSSGSSVFIEPKPVLYLNNELEVLTIQENDEIEAILLKLTNDLRPYLDRLNASFQALV
                     ELDILHAQTQLVRKWQASVPRVGERDLLIKDGRHPLLGEKAVPFSLHLSPDKKIMVIS
                     GPNAGGKTVLLKSLALIVYLAHCGIPVPVNPGSSLPFYHHLFVDIGDHQDIEQNLSTF
                     TYRLSAFQKSLSQISSDSLYLIDEIGSGTDPNEGSALAIGMIDFLSQQGTTCIATTHY
                     PLIKSYVSQHSSMISASMEFNPVLFQPTYRLLIDEIGESYGIKIAEKIGIPQSIIKTA
                     LQQLQKEWIDLNELIISNRKKNQELTSQLIEWQEKVEDATNRQKEVDHLQEQLESQKK
                     LLIKDFQEKLTQYLKKTRDDISQLIGQLRKEKTLDVSVYQELKERIDPKFYLNQELSC
                     SSSEESRKDSPQLAMAFQAGEKVMVDIFHQEGEIVSVDDLKNEAVVLVNGRKCVLSIG
                     HLQKKSEEEEFQSVIQRSYSFQPSLNNVRNQIEIRMMKAEAAREKLEKYFDQVLLAGF
                     KTVYIIHGKGEGILRKVAQEFLQDNPVVESFRNGLPEEGGLGVTVVILKD"
     gene            complement(7361..9292)
                     /locus_tag="PK171_00745"
     CDS             complement(7361..9292)
                     /locus_tag="PK171_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOT04763.1"
                     /translation="MKKRRRARSKFFRFSFFSAFVFGILAGIIFFILVQFFYLDDIRV
                     ISSQIDNFRPSFTTRVYDNQDRLIHELYTENREYVALADIPELLQKAFIASEDQNFYQ
                     HPGIDISGIIRATLQNILNRRIVEGASTITQQLARNRFLSHERIFNRKIKEQVLALFI
                     ERKYTKDQILEAHLNQIYFWHGVYGVKTAALVYFGKNLSQLNLAEIAMLVGIQRSPGN
                     FSPYVNLRAAQIQQRRVLRLMTERGYIDQSEMARAIETPIVLSGLKNIPSGAPYFIDH
                     ILKELLAKYDGETVFNGGLKVYTTLDLDVQKVATGALQESGYQGAILCMDPKSGEILA
                     MVGGKDYQESKFNRATQAYRQPGSTFKPFIYTTAIENGITAVDWYVDEPLQFPDGWKP
                     KNYDKTFRGAIQIHEALEQSINIIAIKLLQQLGTDQVINYAKRMQVKSQLQKNLSLAL
                     GTSEVTLMELVNAYCAFANEGRIPEPYAITKIVDWEGNVIYSSNKTVRQAIPADTAYI
                     MARLLQGVIERGTARRANIGRPVGGKTGSTEDFIDALFIGFTPDMVCGVFLGNDDRKP
                     LGEAKTGGIIAAPIFAKVMKAAHENLPKEDFIRPESVVERSVCLKSGLLPSSSCKKTI
                     VLPFKSGTVPTKVCNQCSE"
     gene            9465..>9789
                     /locus_tag="PK171_00750"
     CDS             9465..>9789
                     /locus_tag="PK171_00750"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOT04764.1"
                     /translation="MKKLPRMALIAALYVAFTILPPFYTFSYGPIQVRVAEAFTVLPF
                     LFPETIYGLTIGCFLANLLGQVGFLDFVFGTLITFLAALVTSKMKKAYLAPLPPVLFN
                     AFGVSL"
BASE COUNT         2942 a         1876 c         1949 g         3022 t
ORIGIN      
        1 tcgatgacca cgattatcag gtaaaattaa aaagcgctat tcggtttctg gaagatggtg
       61 atcgggttaa atttactatt aggtttcgag tacgagagtc cgcttatgtc gataaaggtg
      121 tattattact tcagaagata atcgaagact tgaatgacct cagtaaaatt gaacaagaaa
      181 taaaaaacga gggtcggaat cttaccatga ccattgctcc caagaaatga ggaggatcaa
      241 ttatgccaaa aatgaaaacc catcgcggtc tggccaaacg ggtaaagatt accgcgaaag
      301 gaaaaataaa ggtggctcag ggaggaaaga gccatcattt acatcaaaaa gaacaagatc
      361 gtaaaagacg gctacgtaaa acggtgattg ttgataaaag tatagaaaag cggattaaga
      421 aacttttacc ctatgcttga ttatgggcat cagtactttt caaatagact atagtgcatg
      481 aattttataa aaaagccagg gttgaataag tgaaatttgg ttggaaagat aggtgatttt
      541 cgttgccaag agttaaaaat agtgtggcat cacgacaaag aaaaaagaaa ataatgaaat
      601 tagccagtgg ttatcggggt tctcgttccc gttcctatcg gcgagcaaat gaacaagttt
      661 taaagtctca attctatgct tatcgtgatc ggaaaaacaa aaagagagac ttccgacgct
      721 tatggatcgt tcgtattaat gctgcagttc gggaacatgg gttaaaatac aaccagttta
      781 tatttgctct aaaaaaagcc ggtattgaga ttgaccggaa aaatttatcc aatattgcca
      841 taacggatcc agtggcattt gcgcatctcg ttgaggttgc caagtcagca acctaattct
      901 tttattaagg tgaaagttaa aataaaatgc gataccactc tccaaatggt atcgcatttt
      961 attttaatgg ttttttcgcg agtggacctc tggttgatat tgtcgatggg aggaagaggt
     1021 gttgattaat ttaaacaatg ttcttgaaaa atttcagaat gaaattgaac agacaaaaac
     1081 ccttgatgat ttaaatcgta taaaggggaa atatataggc cggaagggac agctcaatga
     1141 attatttaaa gaaataactc agctttcttc cgatgaaaaa aaggattggg ggaaaaaaat
     1201 caatcattta aaaggattga tagataatca acttcaggag aaattaaaag aacttaccca
     1261 gacattaaaa acgtctatta aggttgatat ctccctaccg ggaaaaaaac cttgggtagg
     1321 gtcattccat cctattcaat taactctgca tcaaatccta actattttcc aaagtttagg
     1381 ttttcgaatt gagtcaggac cggaaattga aagtgagtac tacaactttg aagcactcaa
     1441 ttttccaccc gatcatcccg cccgggatgc tcaggattct ttttttctcg ataaagaata
     1501 tttattgaga actcatacct caccatctca aattcgaatt atgcaaaaaa ttcaaccccc
     1561 ttttaaagta gttgtacccg gaaaatgttt tcgacgggat gccatggacg cctctcatag
     1621 ccccatgttt caccaagttg agggcttggt ggttgcagaa acaatttcca tgggcgattt
     1681 gaaaggaacc atcgaagtgt tcgcccaacg cttttttggg aatgaccggc aacttcgctt
     1741 ccgtcccagt tttttccctt ttaccgaacc aagtgctgaa gtcgacattt cttgtggttt
     1801 gtgtcaaggg aagggatgtc gttcttgtgg ccaatcagga tggttagaaa tcatgggagc
     1861 aggattggtt catcccaaag tttttgaagc tgccggatac aatcccaatc aggtcagagg
     1921 ttttgccttt ggaatgggaa tcgatcgtgc tgctttatta aaattcggta ttcccgatat
     1981 ccgttggcta tttgaaaacg atatgtcact tatcgaacaa tttcatgcta ttcaatagga
     2041 gtatccaaat acaatgaaag ttgcctattc aattctcaaa cgtttcgtac caaatctcga
     2101 ctgtccggct gaaaaaatag ccgagattct aacatctcag ggttttgtga tcgagagcat
     2161 aacgagcgct actgacactt tttctcccaa tttaaccgca gcgaccatcg tcaaacggaa
     2221 atccggaatt ctttacgatg tttgtactgt ccaaatcaaa gatcggcagt atgatgttaa
     2281 atgtgaaaag tggggaatac cggatatcgg aaaaaaggtt atagtcaatc caattggtga
     2341 tttgataagc acccagcctc ttcgtaacca gcgtatacca gaaaaagaag aattttttat
     2401 tgaaatccca gctaccatcg aagctgaaga gggagaactc atttcacctc aaatcatagg
     2461 agaagattgg gtttttgatg tcgaaatcac tgccaatcgc ggagattgta tgtcaattat
     2521 cggcttggcg cgggaaatag cggcaggtct cgatttagaa taccaaatac catctcaaca
     2581 cttggacgtt gatgattcga tcagtaactt tcaagtcaac atagacgatc aagacctctg
     2641 tcctttttat agcggccggt tagctaaaaa ttttaaaatt cagccttccc cttggtggat
     2701 cgttagcgaa ctttatcttt tgggacaacg tcctatcaac caggtagttg atgtgactaa
     2761 tttagtcatg atggaaactg gtcagccact tcatgctttt aacgcctctg ggatattcga
     2821 tcaaaaaatt gttgtgagaa gagccaaaaa taatgaatca ttgataaccc tcgatggaat
     2881 agaaagaaag ctcaccaata atatgttagt gattgctgat gccaatcggc cggttgctat
     2941 cgctggtatt atgggaggat atgaatcaga agtttctcct gacactcagg aagttttctt
     3001 ggaagcggcg atatttaacg ggagtcaaat tcgtaaaaca gctcgggaat taggattacg
     3061 aaccgaagcc tccgcgcgct ttgaaagggg agttgatcca ggatcggtat tctatgccag
     3121 tgagcgtgcc ctatacctcc ttaaagaaat tgatccttct ataaagattt taaaagactg
     3181 ggtagtaagc ggagaagtcg tttcgtcaga accagaaatt gaattttcat cttcctgggt
     3241 tgaagaaatc atgaactgta aaattccgct ggaaaaaatg caaacggttt taactaaatt
     3301 agggtttgtc cttctttctc aacccaacaa ctccataatc aaagtcaaag tcccttcctg
     3361 gcgaccggac gttcaacaac cgatcgactg tgccgaggaa gttggccgta tcttcggtta
     3421 cgatcatatt cgatctcaaa ttcccagttt cccctttgat cccggcaatc ctcgtcggga
     3481 actgttatgg gaaggtgttc tccgaaatca cttgcgctca ataggcttaa aagaatgcgt
     3541 ttcgctttct ttgaccagtc aggccaattg tgatctttta ggtattcccg aggctgatgt
     3601 gatccgggtt ttgaatccac tttcacagga tcatgtcatt cttcgtccgg atttgatcat
     3661 cagtgctttg gatatcataa aaaccaacat cgctcgagga cgagaaaatt ttgggttttt
     3721 tgagtatggg gaagtatttt ctcgaaccaa cgacttggat aatccctatt gcgaagagtg
     3781 gcggttggtt gtcgttttga gtggtcattc ttatcctgct tattggcaac aattttccca
     3841 gatagacatt ttttccttaa aaggttttgc cgaaagcctc attgagctta tcggataccc
     3901 gcttcagatg gtaaattggc aacctttaga tgaaaatcct tattttgatt cgagtctttc
     3961 ttttcgcggt atactcaaaa atggtattga aatttttcga ggaggactga tttgtaaaac
     4021 catcagtgat gcttttggtt tctggggaga gcattactgt ttagaggttg cctggcaaaa
     4081 actttttgat ggtatcaaac tgaatgattt tcaactccag gaaatcaacc gatatccttc
     4141 aatccggcga gatatttcca ttgttgtcaa tcaagaccag acttggaaaa atatcgagtc
     4201 cttggtcctg aaagtagctt cttcacatga tttacctatg gagagagtcg aactttttga
     4261 ctcttaccaa ggaaaacacc ttcctccggg gaagaaaacc ttttcttatt ccattgtttt
     4321 ccgatctaca cataaaactt taaccgataa cgaagtcgat gaatgggtaa acatcattaa
     4381 agcagccatt aaaaaagaac cggaattatt ccttcgggag gaactcgcag gaggattcta
     4441 atcgactggt atagcataat ctgtattttt attctcattc ttaatgttat tcgaagttca
     4501 atccggggtt ttagtaaaga gattctggct ttgacaggag tagtgattgg gcttttgagt
     4561 gccttacggt ttcaccagaa cctaggtcag tttttgacca atctctttca ttgtaattca
     4621 gtctggataa acttgattag tttttttgtg atcttcattc cagtcgttct tttgttttcc
     4681 tgggtgggta tgttttttcg aaaagttttc gaaggtcttg atattgtatg gttcgatgcc
     4741 attctaggtt ttgttattgg tatcttgaaa ggttttctct ggatctccat catcactctt
     4801 tttgtgctta atgtttcttt tctcgaattt ctcaataatg gtatttatca atctcgtttt
     4861 tatcaaagtt ttacccagcc aatcattgtt tttattgatt cgatggttca aaaaattcct
     4921 gaattttcct ttcttaatgc ttatttagaa aagggtttaa accaaagtga cgatgaactt
     4981 atccaaagat atactgaaga attttgattt tgataaaata cttcatcagt taagctctgc
     5041 ctgtgtttgt gatgtttctc gcgaaaaaat gatggaattg caaccctata ccaatccaga
     5101 gataatcaac caaaggttgc aaaccgtcca acaacttctt gattttatca cttttgacgg
     5161 cagcttagat ctttctggat taaccgacct tcgtgaaatt tttcaaaaaa ttgccattcc
     5221 aggatcggct ctcaatttaa atcaagtcat tcaggttttt cattttttac tcttatgtga
     5281 aaaaaccaaa catctgcaaa atgaaaccag aataaaagag aagtatccgc ttatcaaagt
     5341 ttttttcgat gctattgaaa gctatgctcc tcttattaag gaaataaaat tgatctgttc
     5401 acctgatggg ttgattctcg ataccgccag tcctcgtctc cgcgatattc gaagacgtct
     5461 gaaccgtctt caagtagaaa ttcgaaccac cattgaaaat tgcctacgca atcgggatat
     5521 agctccttat cttcaagata caacctacac cattcgacgg gggagatatg tgatccccat
     5581 taaatccgag tttcgaggac gcatcgatgg gattgtagcc gattattctt cgtctggttc
     5641 gtcagttttt attgagccaa aaccggttct ctatttaaat aatgaattag aagttttaac
     5701 catccaagaa aatgatgaaa ttgaagcaat tctgctaaaa ttgaccaacg atttgcgacc
     5761 ttacctcgat cggcttaatg cttcattcca ggctctagtc gaactggata ttctccacgc
     5821 tcagacacaa ctggttcgta aatggcaggc gagtgttcct cgggtaggtg aaagagatct
     5881 cctcatcaag gatggtcgac atcctctttt aggagaaaaa gcggttcctt tctcgcttca
     5941 tttgagtccg gataaaaaaa tcatggttat tagcggaccg aatgctggcg ggaaaaccgt
     6001 tttgttaaaa agcttagctt taatcgttta tcttgctcat tgtgggattc cagttccggt
     6061 taatcctggc tcttccttac ctttttacca tcatcttttt gtcgacattg gagatcatca
     6121 ggatattgaa caaaatctca gtacctttac ctatcgtctt tcagcttttc agaaatcgct
     6181 ttctcaaata tcatctgata gtttatattt aattgatgaa attggatcgg gaaccgatcc
     6241 caacgaaggc tcggcgttag ctattggaat gatagacttt cttagtcaac aaggaacaac
     6301 ctgtattgca accactcatt atcctttgat aaaaagctat gtttctcaac actcctctat
     6361 gatttcggca tcaatggagt tcaatccagt tcttttccag ccaacctatc gactcttaat
     6421 cgatgaaatt ggggaaagtt atggaattaa aattgctgaa aaaattggta tcccgcaatc
     6481 gatcattaaa actgctttac aacagcttca aaaagagtgg atcgatctta atgaattaat
     6541 catctccaat cgaaaaaaaa atcaagaatt aaccagtcaa ttaattgaat ggcaggaaaa
     6601 agtggaagat gctaccaatc gacagaaaga agtagaccac cttcaggaac aactggaatc
     6661 acaaaaaaaa ctacttatta aagatttcca ggaaaaatta acccaatacc tcaaaaaaac
     6721 ccgggatgat atatcacaac tcattggtca attgagaaag gaaaaaactc ttgatgtaag
     6781 tgtttaccag gagctaaaag aacgaatcga tcctaaattt tatttgaacc aggagttatc
     6841 ttgttcttct tcagaagaga gcaggaagga ttctccacaa ctggctatgg ctttccaagc
     6901 tggggagaaa gtgatggttg atattttcca tcaagaagga gagattgtat ctgttgatga
     6961 cttaaaaaat gaagccgtgg ttttggttaa cggccgtaag tgtgttctct ctatcggtca
     7021 tcttcaaaaa aaatctgaag aggaagaatt tcaatccgtt atccagcgga gttattcttt
     7081 tcaaccatct ttaaataatg taagaaatca aattgaaatt cgaatgatga aagctgaagc
     7141 tgcccgggaa aaattggaga agtattttga tcaagtcctc ttagctggat ttaaaactgt
     7201 ctatatcatt cacgggaaag gagagggaat tttaagaaag gttgcccaag aatttctaca
     7261 agataatcct gtggtagaaa gctttcgtaa tggcttgccc gaagaagggg gtctgggggt
     7321 aactgtcgtg attcttaaag actaatttaa aataaatgat tcattcacta cattgattac
     7381 agaccttagt cggcactgtc cctgatttaa aaggcagtac aatagttttc ttacaggaag
     7441 aggagggaag aagccccgac tttaaacaaa ccgacctttc aaccaccgat tctggtcgga
     7501 tgaaatcctc ctttggcagg ttttcatggg cggctttcat cactttagca aaaattggag
     7561 ctgcaataat tccaccagtt tttgcttctc ccaagggttt gcggtcgtca ttgcccagaa
     7621 aaaccccaca aaccatatcg ggggtaaatc caataaacag agcgtcaata aaatcttcgg
     7681 ttgaaccagt ttttccaccg accggacgac caatatttgc ccgtctggct gttccccgtt
     7741 caataactcc ctggagtaag cgagccataa tataagcagt atcagctggt atagcttggc
     7801 gtacggtttt attgctggaa tagattacat ttccttccca atcgacaatt ttagtaatgg
     7861 cgtacggttc agggatacgt ccttcgttcg caaaagcaca ataggcattg actaattcca
     7921 ttaaagttac ctctgaggtg cctaaagcaa gagataggtt tttttggagt tgtgatttta
     7981 cttgcatcct tttggcataa ttgataacct ggtcggttcc caattgttgt aatagtttga
     8041 tagcaataat attgatcgat tgttccaagg cttcatgaat ttgaattgct ccacgaaaag
     8101 ttttgtcata gtttttcggt ttccagccat cggggaattg gagtggttca tcgacatacc
     8161 agtccactgc agtaattcca ttttcaatag cagtcgtata aataaagggc ttaaatgttg
     8221 agcctggctg tcgataagcc tgagtcgctc gattaaattt actttcttga taatctttcc
     8281 ctcctaccat ggctaaaatt tctccacttt tgggatccat gcaaagtatg gcgccttgat
     8341 aaccactttc ctgcaaggca ccggtcgcaa ctttttggac atctaaatca agagtggtat
     8401 aaacttttaa tccaccgtta aaaactgttt ccccgtcgta tttggccaat aactccttta
     8461 aaatgtggtc aatgaaatag ggcgcacccg atggaatatt tttaagtcca gaaagaacga
     8521 taggcgtctc tattgctctt gccatttccg attggtcgat ataccccctt tcggtcatga
     8581 gacgtaaaac ccgtctttgt tgaatttggg cagctcgaag attcacatag ggtgaaaaat
     8641 ttcctggaga acgctgtatc ccaactaaca tagcgatttc cgccaaattc aattgggaaa
     8701 ggtttttccc aaagtaaact aaagcagcag tttttactcc ataaactcca tgccaaaaat
     8761 aaatctggtt aagatgagct tctaaaattt gatctttggt atactttctc tcgataaata
     8821 aagccaaaac ctgttcctta atttttcggt taaaaattct ttcatgggaa agaaaacggt
     8881 tccgagctaa ttgttgagtg atggtactgg ctccttcaac aattcgtcgg tttaaaatat
     8941 tttgaagagt tgctcttata ataccgctga tatctattcc tggatgctgg taaaagtttt
     9001 ggtcctcact ggctataaaa gctttttgga gcagttcagg gatgtcagca agagcaacat
     9061 actcacggtt ttcggtataa agttcgtgga ttagccgatc ttggttatcg taaacccggg
     9121 tcgtaaaaga tggtcgaaaa ttatcgatct gactggaaat aactcgaata tcatctaaat
     9181 aaaaaaattg aaccaatatg aagaaaataa taccagctaa aataccaaaa acaaatgctg
     9241 aaaaaaatga aaaacggaaa aattttgatc gagcacgtcg tcgctttttc atttttaaag
     9301 aggtaccttt taattcttta ttcatctatt ttcctatatt tatgaacaaa agttttcgtt
     9361 tttttggatt ttgatataat ttcactaaaa ctttattata tcattcaata ccttttttta
     9421 ggtattttat ttgaaaaagt caaataattg gataggagaa ttcaatgaag aaacttcctc
     9481 gtatggcatt gatcgctgcg ctctatgtcg cttttaccat tcttccacct ttttatactt
     9541 tttcatacgg accgatacaa gtcagagtag cagaggcttt tactgtcttg ccttttctat
     9601 ttcctgaaac aatttatggt cttaccattg ggtgtttcct agctaatctt ttggggcagg
     9661 ttggcttctt ggatttcgtc tttggtactc tcattacttt tttagctgcc ctggtcacct
     9721 caaaaatgaa aaaagcttac ttagcgccgc ttcctccagt cttattcaat gctttcggtg
     9781 tttcattat
//
LOCUS       DAOSOL010000014         9234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_7432_length_9234_cov_3.936921, whole genome shotgun sequence.
ACCESSION   DAOSOL010000014 DAOSOL010000000
VERSION     DAOSOL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7432_length_9234_cov_3.936921"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /gene="tsaE"
                     /locus_tag="PK171_00755"
     CDS             complement(<1..279)
                     /gene="tsaE"
                     /locus_tag="PK171_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT04765.1"
                     /translation="MTHLDTLLSSNEDQTMKIASHFTQEYFQPGVPILLSGQLGAGKT
                     YFTKGIAEALGISSREVISPSFALINEYHGPVCILNHMDFYRLENWQEV"
     gene            complement(276..1412)
                     /gene="alr"
                     /locus_tag="PK171_00760"
     CDS             complement(276..1412)
                     /gene="alr"
                     /locus_tag="PK171_00760"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT04766.1"
                     /translation="MNIDYPLWMEIDSQSLHNNFTRILQHLSIPNASRIIGVVKADAY
                     GHGIESVLLLQKWGMNRFGVATVQEGEALRQLGIKGTIYLLGGFINQEIPGIFKNHLT
                     PALSSREEWDQLKSAVQLNQQELEFHLKIDSGMYRMGFLPEELDESFIKQMSHHPYLH
                     LKGVMTHFASSENDPTYTHRQFEIFQNFLQKKFLSEQVSIQNLEKHCCNSAGFLFFPH
                     YHLDMVRVGIALFGVSPIQDPSPVRSLGLSPVMRIKSRVKLLKHLPSGCCIGYGGTCI
                     TERPTRLAVIPIGYAQGLLRRLSNRMEVLIKGQRAKCLGVISMDQMMVDVTSIPDVKR
                     GDLVTILGSDGPDEIRAEEMAAWAETIPHEILCSFIRIKNRIVV"
     gene            complement(1378..2967)
                     /locus_tag="PK171_00765"
     CDS             complement(1378..2967)
                     /locus_tag="PK171_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOT04767.1"
                     /translation="MKLITSESMQKLEKRLVESYRIPTQLLMENAGRNIVDKIRQIGI
                     DKSWNQVVVVCGPGNNGGDGLVIARHLNLRYPLLPITIFLVGNPERLKNDTALNYEIC
                     QRIGLPIQRVFPGNPPRFDKGTLIIDSLFGTGLSKKVEGVFQEVIDAINKAKESMIIA
                     VDAPSGVDVTWGKIMGRAVKAHHTITFGMLKRGLVVAPAREYCGTIHLTDIGIPLDRE
                     PADVPQEGFLITPDFIRPFLPEKAFLSNKISAGVVMVVAGSPAMTGAGLLTCQAAYRS
                     GAGMVIWATNHQLAPLVKSSIPEVVSTILSSNKKNTQWKYLPHHADEICEAILQRKCR
                     SIAIGPGLGSSPASRYFIEKVIEKSPIKGVIDADALNAIAVNREFWKNRLQGWILTPH
                     EGEMGRLLNRSASSIAENRIAASCALSQYFNCITVLKGPGTIVVSPQGEIAINPTGNP
                     NLATAGSGDVLTGIIAAFLAQGGSPFISAVTGVFLHGLSADLYQKQSPQLLASELASY
                     LPYAIKVVKNCEYRLPIVDGD"
     gene            complement(2970..3368)
                     /gene="acpS"
                     /locus_tag="PK171_00770"
     CDS             complement(2970..3368)
                     /gene="acpS"
                     /locus_tag="PK171_00770"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT04768.1"
                     /translation="MNPKIYIGIDLINLTRVKKTLSRCGERFVKRIATSAELEYYCDV
                     SERRFIEGVASLFAIKEAVKKIFLQKDLNPNWKQIQVFHHSSGKPWVSLSEESLKQIF
                     ESISLSLSHTAEMVVAVAVGVERRIDNTEV"
     gene            complement(3365..5452)
                     /gene="fusA"
                     /locus_tag="PK171_00775"
     CDS             complement(3365..5452)
                     /gene="fusA"
                     /locus_tag="PK171_00775"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT04769.1"
                     /translation="MKKYDSKDIRNVGLFSHAGAGKTTLAETLLYHSGLISRKGKVEE
                     GNTVSDWEPEEIKRGISIDLSVLPFEWQGKKINLIDTPGYADFVGNVLGALRAVDCGL
                     IPVCGVSGVEVGVERSWKYLEQNQLPVFLFINKMDREGANFDKVLSEIQSELSAKATP
                     LYIPIGKESNFQGVVDILAMKALYFQADGKSFKEDTVPADLQNQAQEIHDFLVENIAE
                     TNDELLNMYLEGQTIEPELLKETLRQAILQRKLFPVLCGSGLTDRGVSLLGDALSHYA
                     PSPLDRPPIQGINPKTKEEVERKCNETEPFSAYVYKIISDPYVGKLALVRVFSGKLTS
                     DSRLFNASRDTEEKVGQLLTLRGKNQESVTEIGPGDMGAIAKINEIYLGDTLSEKEKT
                     IVFANLEYPEANYLSSIKPLGRGDEDKISLGVSRMLEEDPTVKEVRDPDTKEDVLYGY
                     GDIHLDVLIEKMKRKFGVEVGLAIPKVPYKETIKGTTKVEGKYKRQSGGRGQYGHCWL
                     ELEPLPRGKGFEFVDKIVGGAIPRNYIPSVEKGVQEAMLEGVLAGYPVIDIRVIVYDG
                     SFHPVDSSDMAFKIAGSMALKKGCLECNPVLLEPIMNLEITVPEEFMGDVIGDINSRR
                     GRILGMDPQDGYQLIRANVPLAEIFRYSVDLRSITQGRGSYKMTFSNYEEVPAQIAEG
                     IIQKAKKEKEEAG"
     gene            complement(5454..5714)
                     /locus_tag="PK171_00780"
     CDS             complement(5454..5714)
                     /locus_tag="PK171_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04770.1"
                     /translation="MKKFKTWYHHDRETKWYAVGFAVALWFYLLSENYAQVGYSWQKV
                     WNHSTIIPITTTQQTLPGDTIPGAILEEIKPIDNDKTVKGGN"
     gene            complement(5753..6514)
                     /gene="cdaA"
                     /locus_tag="PK171_00785"
     CDS             complement(5753..6514)
                     /gene="cdaA"
                     /locus_tag="PK171_00785"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOT04771.1"
                     /translation="MNLSVHWRWTIELILILYLSFRLFSITKGTSLYSVLKFLLILFV
                     IAGLANFSGLKELNFIWKIILVAYIGGAFIVFQPELRRLYVNRAYHRPDSTSRFFFHS
                     EEDRTKFIDEITITCQTLSRKKVGALVVLERNNDLRDFLQTGIAIDAVFSSELVYSIF
                     LTESPLHDGAVIIKENRIIAAGCILPLAEKTEVKKLVGTRHRAGIGITEQTDALSLVV
                     SETTGKVSIAVQGKMAWDVETSTLKKMLRILYRKI"
     gene            complement(6530..7465)
                     /gene="argF"
                     /locus_tag="PK171_00790"
     CDS             complement(6530..7465)
                     /gene="argF"
                     /locus_tag="PK171_00790"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT04772.1"
                     /translation="MRWVFKGRDFLSLSDYSADEIYYLLETAIVLKRRWMIGEKIEIL
                     SGKTLAMLFEKPSLRTRVSFEMAMHQLGGKAMYIGPQEVGLGKREAIKDVAMVLDRMV
                     DAIMIRTFSHNNLIQMAKYSQIPIINGLSDLHHPCQILGDILTILEKKGTLKGQKISY
                     FGDGNNVCHSWLVAAGVLGLNLTICSPNNYQPQTEIWDWAQEKGSNSGATIIYEADPE
                     MAAQDADVIYTDVWASMGKESEMEERIRHFQKLQVNQNILDKARSNAIVLHCMPAHRN
                     LEITDEVMDGPRSVVIDEAENRLHAQKALLALIMS"
     gene            complement(7481..8614)
                     /locus_tag="PK171_00795"
     CDS             complement(7481..8614)
                     /locus_tag="PK171_00795"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT04773.1"
                     /translation="MFFGFPKFPVKIKKTLVVVGLYPLLEINLSKLKQNIETLQKKCS
                     EWMLKPVAVTKGFCADYKITDLLYGSGIRSFADSNLSNLLKLIDRFGHKAQYQLIRLP
                     MKEEINVIIENQIIPLVSQLEILNLFAQKAKQFHAQSSVILAIETGDGREGFLPEEVL
                     NNIDQIVSLDNIAIIGIGTSLACLSGVLPQIKTLEELSSLRNQLRLFLHNPHLFISVG
                     GTTFFSLWEECSQPPPVDQIRCGEAFLFGSDISRKRNLNWLQQGAFHIEAEIVEIKNK
                     QPDSSDRGFDAFGRPGKMSSEKNYPRKRALVAVGLQDIDDTQLFFEDRNVTIIGATSN
                     YLVLDCEESQHDYRIGDLVRFQAGYGTVLRAFLSPYVQKRYIE"
     gene            complement(8665..9054)
                     /gene="rpsI"
                     /locus_tag="PK171_00800"
     CDS             complement(8665..9054)
                     /gene="rpsI"
                     /locus_tag="PK171_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT04774.1"
                     /translation="MDMKYYATGRRKTSIAKVWLTLGSGKVVINGRELKDYFPQPVLQ
                     ILATRPLALTGSDSRFNVNVIVEGGGLSGQAGAMALGISRSLIKIDENLKPTLKKARL
                     LTRDPRMKERKKYGQRSARARFQFSKR"
BASE COUNT         2664 a         2035 c         1802 g         2733 t
ORIGIN      
        1 aacctcctgc cagttttcaa gacgataaaa atccatatgg ttgagaatgc aaactgggcc
       61 atggtattca ttgattaagg caaaacttgg actaatcact tcccggctgg aaatacccag
      121 cgcttcagcg attcccttgg taaaataggt ttttccagca cctagctggc cggataagag
      181 aataggaact cctggctgaa aatattcctg agtaaaatgg ctggcaattt tcatggtttg
      241 gtcttcattg gatgataata gagtatcaag atgagtcata ccacaatccg atttttaatc
      301 cgaataaaac tacataaaat ttcgtgagga atggtttctg cccaagcagc catctcttcc
      361 gctcgaattt catcaggtcc gtctgatccg agaattgtaa ccaggtctcc ccttttcaca
      421 tcagggatag aggttacatc taccatcatt tgatccatgg aaattactcc taagcatttg
      481 gctcgttgtc ctttgattaa aacctccatt cggtttgaaa ggcgccgcaa aagcccttga
      541 gcatagccga taggaataac tgccaaccga gtcggacgtt cggtaataca ggttccacca
      601 tacccaatac aacatcccga tggaaggtgt tttaaaagtt taacccggct ttttatcctc
      661 atcacgggtg ataaaccaag tgaacggact ggagaaggat cctggatggg tgaaacacca
      721 aataacgcaa ttcctaccct caccatatca aggtgataat gtgggaaaaa taggaaacca
      781 gcgctgttac agcaatgctt ttctaaattt tgaattgaaa cctgttctga aagaaatttt
      841 ttttgtaaaa agttctgaaa aatttcaaac tgccgatggg tataggtagg atcattttct
      901 gaactggcaa aatgggtcat tactcccttt agatgcaaat aggggtgatg actcatttgt
      961 ttgatgaatg attcatccag ttcttccgga agaaagccca ttcgatacat ccccgaatca
     1021 atttttaaat gaaattccag ctcttgttga ttcaattgaa ccgcagattt cagttgatcc
     1081 cattcttctc gggaggaaag ggcgggtgtt aagtgatttt taaaaatccc aggaatttcc
     1141 tggttaatga atcctcctaa taaatagatg gtccctttga ttcctaattg acggagagct
     1201 tcaccctctt gaacggtagc aaccccaaag cgattcatgc cccacttctg aaggagcaaa
     1261 acgctttcta taccatgccc gtatgcgtca gctttgacta ctccaattat ccgagaggca
     1321 ttgggaatac ttaggtgttg aagtattcga gtaaaattgt tatggagcga ttgtgagtca
     1381 atctccatcc acaatgggta atcgatattc acaattttta accaccttta tcgcgtatgg
     1441 tagataagat gctagttcac tggcaagaag ttgaggagac tgtttttgat ataaatcagc
     1501 tgataaccca tggagaaata cgccggtcac ggcactgata aacggagacc caccttgagc
     1561 caaaaaagct gcaataattc cggttaaaac gtctccactt ccagcagtag caagattggg
     1621 attccccgtt gggttgatag caatttctcc ctggggagat acaactatgg ttcctggtcc
     1681 ctttaaaacg gtaatacagt taaagtattg agacaaagca caactcgcgg cgattcgatt
     1741 ttcagcgatt gaagaagccg agcggttaag gagacgaccc atttcgcctt cgtgaggagt
     1801 aagaatccaa ccttgtaaac gatttttcca aaactcccgg ttaacagcaa tagcatttaa
     1861 agcatcggca tcaattacac ccttgatcgg tgatttttca atgacttttt caataaaata
     1921 tctggatgct ggagacgaac ctaatccggg accaattgca attgagcggc attttcgttg
     1981 taagatggct tcacatattt catcggcatg atgaggtaag tacttccatt gagtattttt
     2041 tttattggaa gaaagaatgg tgctcaccac ttcaggtata gaggatttca ctaaaggtgc
     2101 cagttgatga ttggttgccc aaataaccat tcctgctccg cttcgatagg cggcttgaca
     2161 ggttaaaagg ccggctccgg tcatggccgg gcttccagca accaccataa caaccccggc
     2221 acttatctta ttactgagaa aagctttctc aggcaaaaaa ggacgaataa aatcaggggt
     2281 gatcagaaaa ccttcttggg gaacatctgc cggttctcga tccaagggaa ttccgatatc
     2341 agtgagatga attgttccac agtattcacg agcaggagct accaccaagc ctctctttag
     2401 cattccaaaa gtaatggtat gatgagcttt aacagccctt cccatgattt taccccaggt
     2461 gacatcgaca cccgaagggg catcaacggc aataatcatt gattccttgg ctttattgat
     2521 agcatcgatt acttcttgaa aaacaccttc aacctttttc gataatccag taccaaagag
     2581 ggaatcgata attaaagttc ctttatcaaa gcggggggga tttcctggga atactctctg
     2641 aatgggtaaa ccgattcgct gacagatttc ataattaaga gcagtatcat tcttcagccg
     2701 ctcgggattt cctaccaaaa aaatggtgat aggcaataac ggataacgaa gattgagatg
     2761 gcgggcaatg accaatccgt ctcctccatt gttacccgga ccacagacca ccactacctg
     2821 attccaggat ttatctattc caatttgtct aatcttgtcg acaatatttc gcccggcatt
     2881 ctccatgagc agctgagtcg ggatccggta ggattcgact aatctttttt ccagtttctg
     2941 catggattcg gaagtaatca gtttcactgt tagacctcag tgttatctat gcgcctttcc
     3001 acacccaccg ctacagcgac caccatctca gcggtgtggg aaagggatag ggatatactt
     3061 tcaaatattt gttttaatga ttcctcggat aatgaaaccc aaggctttcc agaagagtga
     3121 tggaaaacct gaatctgttt ccagtttgga tttaaatcct tttgaagaaa gattttctta
     3181 acggcttctt ttatagcgaa gagagaagcg actccttcaa taaaacgtcg ctcgctcacg
     3241 tcacagtaat attccagctc agctgaagtg gcaattcgtt ttacaaacct ttctccacat
     3301 cggctaaggg ttttttttac tctcgtgaga ttgatgagat cgataccaat gtatatcttt
     3361 gggttcatcc ggcctcttct ttttctttct tggctttttg aataatacct tcagcaatct
     3421 gagctgggac ttcctcatag ttggaaaagg tcattttata agaccctctc ccttgggtaa
     3481 tggagcgaag gtccactgaa tagcggaaaa tttcggctaa aggaacatta gctcggataa
     3541 gctgatagcc atcctgtgga tccatcccca agattcttcc tctacgactg ttgatatcgc
     3601 cgataacatc tcccataaat tcttcgggaa cggtgatttc aaggttcata attggctcaa
     3661 gcaaaaccgg attgcattcc aggcatccct ttttcaaagc catagatccg gcaattttaa
     3721 atgccatatc cgatgagtca accggatgga aagatccatc gtaaacgata actcggatat
     3781 caatgaccgg ataacccgct aaaacgcctt ccaacattgc ttcctgaacg cccttttcca
     3841 ccgatggaat ataattgcga ggaatggctc caccgacaat tttatcgacg aattcaaaac
     3901 ccttaccacg gggaaggggt tcaagttcca gccaacaatg gccatactgc cctcttccgc
     3961 ctgattgtct tttatatttt ccttccacct tggtggttcc cttgatggtt tccttataag
     4021 gaacttttgg aatggccaaa ccaacttcca ctccaaattt tcgcttcatt ttctcgatta
     4081 atacatcgag atgaatatcc ccataaccat agagaacatc ttctttagta tcagggtcac
     4141 gaacctcttt gacggtaggg tcttcttcca gcattcgact gactcctaag ctgatcttat
     4201 cttcatcacc ccgtccaaga ggcttgatgc tcgataaata attcgcttcg ggatattcca
     4261 gattggcaaa aacgattgtt ttttcttttt ccgaaagtgt atcaccaaga tagatttcat
     4321 tgattttagc gatggcaccc atatctcccg ggccaatttc ggttaccgat tcctggtttt
     4381 tacctcgtag ggttagtaat tgccccactt tttcttcagt atcacgggaa gcgttgaaca
     4441 aacgagaatc agaagttaat tttccagaaa aaacccttac taaagccaat tttcctacat
     4501 aaggatcact gataatttta taaacgtaag ctgaaaaagg ctcggtttcg ttacattttc
     4561 tttcaacttc ttctttggtt ttcggattta ttccttggat gggaggacga tcaagaggcg
     4621 atggggcata gtgggataaa gcatctccga gaagagacac tcctcgatcg gttaaaccgg
     4681 acccacataa cactggaaat agctttctct gaagaatcgc ttggcgaaga gtttctttaa
     4741 ggagctcggg ttcgatggtt tgtccttcca agtacatatt taataactca tcattggttt
     4801 cagcaatatt ctcgaccagg aagtcatgaa tttcttgggc ttgattttgt aggtcggctg
     4861 gtacggtgtc ttccttaaat gattttccat cagcttggaa atacaaggct ttcatggcta
     4921 aaatatcgac gacaccctga aaattgcttt ctttgccgat tggtatgtaa agaggagttg
     4981 ctttagccga gagttcactt tgtatttcac ttaaaacttt atcaaaattg gcaccttctc
     5041 gatccatttt gttgataaaa agaaaaaccg gaagctgatt ttgttctaaa tacttccagc
     5101 ttcgctctac accaacttca acaccagaaa ctccacaaac ggggattaac ccacaatcga
     5161 ctgcacgaag agcacctaaa acatttccaa caaaatctgc gtatccagga gtatcaatca
     5221 aattgatttt ctttccctgc cattcaaaag ggagtaccga taggtcgatt gatattcctc
     5281 tttttatttc ttctggttcc caatcagata ccgtgtttcc ttcctcaact tttccctttc
     5341 gggagataag tcctgaatga taaagcaaag tttcggcaag agttgttttt cctgcaccag
     5401 catgtgaaaa taatcccacg tttcgaatgt ctttactgtc atactttttc aaattaattt
     5461 cctcccttca ccgtcttatc attatcaatt ggttttattt cttcaaggat agcaccgggt
     5521 atggtatctc ctggtaaggt ttgctgggtg gtggtaatag gaataattgt tgaatgattc
     5581 cacacctttt gccagctata tcctacttga gcatagtttt cacttaatag ataaaaccat
     5641 agtgctacag caaaaccgac agcataccat ttggtttccc ggtcatgatg ataccaggtt
     5701 ttgaattttt tcaagctttt tttgatcaaa ttgataatat ctgatatttt aatcatattt
     5761 ttcgatagag aattcttagc atttttttca aagtactcgt ctcaacgtcc caggccattt
     5821 taccctgaac tgcgatcgat accttcccgg tcgtttctga aaccaccaga gatagagcat
     5881 cagtttgctc agtgatgcct attccagctc ggtgccgggt tccaactaat tttttaacct
     5941 cggttttttc tgctaaaggg agaatgcacc cggcagcaat aatccggttt tctttaataa
     6001 tcaccgctcc atcgtgcagt ggtgattcgg ttaaaaaaat tgaataaact aattcagaag
     6061 aaaaaacagc atcaatggcg ataccagttt gaagaaaatc ccgtaaatca ttattcctct
     6121 ccaatacaac taaagctcca actttcttac gagagagagt ttggcaagta atggtaattt
     6181 catcgataaa cttagtccga tcttcctcgc tgtgaaaaaa gaaacgggaa gtactatccg
     6241 ggcgatgata agcccgattg acatagagac ggcgaagctc aggctgaaaa acaataaatg
     6301 ctccacctat ataggcgaca agaataatct tccaaataaa attaagctct tttaacccgg
     6361 aaaaattagc taaaccggca ataacgaata agattaggag gaattttaaa accgaataaa
     6421 gcgaagtgcc cttggtgata gaaaacaatc gaaaagacaa atataaaatc agaataagtt
     6481 cgatagtcca tcgccagtgc acagaaagat tcaaaagata actcctccgc taactcataa
     6541 tcaatgccag caaagccttt tgagcatgga gtcgattttc ggcttcatca ataactactg
     6601 aacgtgggcc gtccataacc tcatcggtaa tttccagatt tcggtgagcc ggcatgcaat
     6661 ggagaacaat cgcattggat cgagctttat caaggatatt ttggttaacc tgaagttttt
     6721 ggaaatgacg aatcctttcc tccatttcac tttcttttcc catgcttgcc cagacatcgg
     6781 tatagataac atcggcgtcc tgagcggcca tctccggatc ggcttcataa attatagttg
     6841 ctcctgagtt gcttcccttc tcttgagccc aatcccaaat ttcggtttgt ggttgatagt
     6901 tattgggaga acagatggtg agattgagcc ccaagactcc agctgctacg agccaagaat
     6961 gacagacatt gtttccatcg ccaaaataag atattttttg acctttaaga gtcccctttt
     7021 tctctaaaat ggtcaatatg tctcctaaaa tttgacaagg atgatgaagg tcagaaagtc
     7081 cattaataat cgggatttga gaatactttg ccatctggat aagattatta tggctgaaag
     7141 tacgaatcat aatcgcatca accatacggt caaggaccat ggctacatcc ttaattgctt
     7201 cccgtttccc caagcccact tcttggggac caatatacat agctttccca cccagttgat
     7261 gcattgccat ttcaaaagaa acccgggttc gtagagatgg tttttcaaaa agcatggcca
     7321 gggtttttcc agaaaggatt tcgattttct ctcctatcat ccagcgtctc tttaaaacga
     7381 ttgccgtttc taaaagataa tagatttcat cggctgaata atcagataga cttaagaaat
     7441 ctcttccttt aaatacccac ctcataatac ccatcctttc tcattcaatg tagcgtttct
     7501 gtacataggg cgataaaaat gcccgcaata cagttccata cccagcttga aaccggacta
     7561 aatcaccaat ccgataatca tgctgacttt cctcacaatc taaaaccaaa taattgctgg
     7621 ttgctccaat tattgtaaca tttctgtcct cgaaaaataa ctgtgtatca tcaatgtctt
     7681 ggagacctac tgcaactaat gctcgtttcc gagggtagtt tttttcactg gacatcttac
     7741 cgggtcgacc aaaagcatca aatcctcgat ctgatgagtc aggttgcttg ttttttattt
     7801 ccacaatttc agcttcgata tggaaagctc cctgttgtag ccaattgaga tttctttttc
     7861 tgctaatatc actaccgaag agaaaggctt ctccacaacg aatctgatcc accggaggtg
     7921 gttgggagca ttcttcccaa agggaaaaga aagtcgttcc gcccactgat ataaaaagat
     7981 gaggattatg caggaatagc cgaagttgat ttctcaaaga acttaattcc tcaagagttt
     8041 ttatttgagg aagaactccg cttaaacaag ccaaagaagt tccaatacca ataatggcaa
     8101 tattatccaa agaaacaatc tggtcaatat tattcaacac ttcttcggga agaaatccct
     8161 ctcttccatc accagtttcg atagcaagga taaccgatga ttgggcatga aattgtttgg
     8221 ctttctgagc aaagaggttg agaatttcca attgggaaac caggggaatt atttggttct
     8281 caataattac attgatttct tctttcatag gaagacggat taattgatat tgggctttat
     8341 gaccaaagcg gtcgatcaat ttcagaagat tggataaatt tgagtcagcg aaagatcgaa
     8401 ttccggagcc atagagtagg tcagttattt tataatcggc acaaaatcct ttggtgacag
     8461 cgactggttt cagcatccac tcggaacatt ttttttgaag ggtttcgata ttttgtttca
     8521 gcttagataa attaatttca agaagtggat acaacccaac caccaccaaa gtttttttta
     8581 tttttaccgg gaattttgga aaaccaaaaa acacctgaaa caaaaagagc ccccagactt
     8641 cgggggctcc gataattaaa aatattaccg tttagagaat tgaaaacgag cccgtgctga
     8701 ccgctgacca tattttttcc gctctttcat gcgaggatcg cgagttaaaa gtcgggcttt
     8761 ttttaaggtt ggttttaaat tttcatcaat tttgatcaat gatctggaaa tacccaatgc
     8821 catggctcct gcctggccgc ttaaaccccc accttctact atgacgttaa cgttaaagcg
     8881 actatcactc ccggtcaggg ctaatggacg ggtagctagt atttggagca ctggttgggg
     8941 aaaataatct ttcaattctc tcccattaat aactactttg ccacttccca aagtcaacca
     9001 taccttagcg atcgatgttt ttcttctccc ggttgcgtaa tacttcatat ccatactcac
     9061 gtcattcact ccttagatca agaggaatcg ggttttgggc tgcgtgggga tgggaatcgc
     9121 cagtgtaaac ttttaatttg ctaataatgc ggtttcccag tttatgatgg ggaatcatcc
     9181 cccatactgc cttacgaatc acttcctcgg gttttctttt cattaaatgc tcca
//
LOCUS       DAOSOL010000015         8998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_7707_length_8998_cov_3.379179, whole genome shotgun sequence.
ACCESSION   DAOSOL010000015 DAOSOL010000000
VERSION     DAOSOL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7707_length_8998_cov_3.379179"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..565)
                     /locus_tag="PK171_00805"
     CDS             complement(29..565)
                     /locus_tag="PK171_00805"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HOT04775.1"
                     /translation="MLADFVLIGIVIGYISRGNFQNLKQANIPYLWLIVLGFLVKFIG
                     PYFSEWSFQWFNIIGMIIVFIGTLFSYKSYGMKIVSVGAFLNILVILFNRGMMPVWLP
                     MVQRLQLHDLANHLENGLYLDYTLLNTSTRLPFLGDTLPYYSLIMRRPFVISIGDYLL
                     GIGLMAFIIFYMRKKYSN"
     gene            complement(555..1805)
                     /locus_tag="PK171_00810"
     CDS             complement(555..1805)
                     /locus_tag="PK171_00810"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOT04776.1"
                     /translation="MKSDKKKHLLDFYIYSVIIFGIALFLYSLLTIDLSHFPLAQIIT
                     FLVLIMVTEALPIHLSPHTSISVSFAIIYAFILLTNPYLVMIATFIGNVLIYMKSGWK
                     KSLFNGAQFAISAFLSGYVFQLLGGYAYAWDQFAFYIAIVISILVFFLSNASLVIIVI
                     SLSTGISIPVLWKKDVNGILLQYFGLFPYSLLLYLIYLRIGYVGLFLFFFPLMIARYS
                     FKLYVETKKVHLELLRALTAALDAKDPYTQGHSARVAKISLAIAEKLNLSDKKQEMIE
                     YAALLHDVGKIGIEDAILRKPGPLTEGEYIIVKQHPVIGFDIVSKVDFLKEIAGLIRS
                     HHERCNGSGYPDGKKQTDLPVESLILAVADVFEALTSDRPYRKAYSVEEALIIMENEG
                     NEYYDFKVIKALKEILQEGFEVAG"
     gene            complement(2139..2825)
                     /locus_tag="PK171_00815"
     CDS             complement(2139..2825)
                     /locus_tag="PK171_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04777.1"
                     /translation="MDVPIDFKAIEDSISCIDGIINVKIVGEGSNITEIHVLSNRSKG
                     PKLLVRDIETLIKARFGVEIDHKKISVVSFDLEEPPHETTVIQERPILWGVGWKKSGD
                     NFQADVEIKLVDKVYRSSLNSKAWDQRERCSLIAQAVIDCMNQIVATPLFSLRGVTVH
                     NYCEFDVAVCLVDYRNFGKAEGTLIGTALLRDEIYETVARSALDAVNRKVNYYHSFHR
                     ENLEKNSFEH"
     gene            complement(2924..3472)
                     /gene="raiA"
                     /locus_tag="PK171_00820"
     CDS             complement(2924..3472)
                     /gene="raiA"
                     /locus_tag="PK171_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT04778.1"
                     /translation="MEVVVRGKNIDVDDLMRDYVTKKLSKLDKFFHKLIDATVVFSLV
                     RGRIKSEVTMTASGIVLRAEGEGPDWRTSFETVDDKLERQVKRYKEKLARRGALKSVE
                     ALSAIEINEILSQTESEEKIPGKLVRTKEFTLRPMTVEDAILQMELLSHSFFVYKDID
                     KNKVQILYKRNDGNYGLIDPIY"
     gene            complement(3478..4431)
                     /locus_tag="PK171_00825"
     CDS             complement(3478..4431)
                     /locus_tag="PK171_00825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HOT04779.1"
                     /translation="MILVVCANPALDRLLVLPELGLDGVNRTSRVEVTVAGKGINVAR
                     ALQTMGLESHLFLFLGGENGNKVARGLSQECLPFDAWPTLGETRLTTIIHEEKIHRHT
                     VINESGPIVPFESSLDLIHSIEKQLKPDDFLILSGSLPRGVRRDLYSVLIHIAHQKKA
                     YSVLDSSGIPFSLALKSIPFLVKPNVKEAEEALGFAILSLGDKIKAVACFQKIKIPLV
                     ILSDGPNGLVVGYHDEVFIVKADNTLRGGGYAIGSGDTLVGGVVAQLSQQVDVKDAII
                     FGTACGLANTFCSGAGVFNLDMAHQFMEHIFIENYYSSKRR"
     gene            complement(4428..5411)
                     /locus_tag="PK171_00830"
     CDS             complement(4428..5411)
                     /locus_tag="PK171_00830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOT04780.1"
                     /translation="MDKKAICLGELLIDFVSTLNGVTLRDAPGFEKAPGGAPANVAVG
                     LSKLGIETYFIGKIGKDAFGDFLRMTLDKYGVKTNYLTTTTKAKTTLAFVSLTEEGER
                     DFVFYRDPGADMLLDQSDIIDLIFSGSGVFHFGSITMTHEPSNSATFKAIQLARKYGY
                     LVSFDPNLRPALWKTLDEAREKMRLGMELSDVVKLNEEEAMFIAETNTVEEAIRYLQR
                     SYNLALVIITLGKEGSILVHQEKSLRVEGFPVHAVDTTGAGDGFVAGLISSLYMFWND
                     LRERKSIPDDTLRYAGRRANAVGALTTLKKGAIPALPTQEEVEIFIEENPL"
     gene            complement(5654..7183)
                     /gene="xylB"
                     /locus_tag="PK171_00835"
     CDS             complement(5654..7183)
                     /gene="xylB"
                     /locus_tag="PK171_00835"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT04781.1"
                     /translation="MNYFLGIDVGTTGCKAILVDENGELIAKGVEEYPLYTPRPNWAE
                     QDPSDWWQGTQQAIKKTLDSVSIDRKAIVGIGLTGQMHGSVFLNNKGEVIRPAILWCD
                     QRTAKECEEITQIVGREKMMRINGNPVLAGFTAPKIYWLKNNEPDHYKRIDKILLPKD
                     YIRFCLTGEFATDVSDASGTSLFDVPRRQWSDEIISDLKLNRNWFPDCFESPEITGYL
                     KREIAQLFGMNDQVIVVAGGGDNAAGAIGTGIVKSGLVSASIGTSGVVFAFSDEVKID
                     MKGRVHTFCHAVPGKWHVMGVMLAAGGSFRWLRDVLGTEEKNLAALLDKDAYEILTEE
                     AAQVEPGGEGLIFLPYLTGERTPHADPYARGVFFGLTLKHRKNEIVRAVMEGVTFGMR
                     DSFEIIREMGIPIGEVMAIGGGAKSPLWRKIQADIYQVPLYQVRVDEGPAFGAALLAA
                     VGKGVFPNVEQACQKAVKVMDKVYPDSQKGIRYNEIYQIYKDLYPALKPFYKRISNIS
                     W"
     gene            complement(7206..8468)
                     /locus_tag="PK171_00840"
     CDS             complement(7206..8468)
                     /locus_tag="PK171_00840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT04782.1"
                     /translation="MAEKIIMPKLGLTMEEGVINKWLVKEGDQVEKGDALFEVATDKV
                     NMEVESTANGVVLKIMAAEGETIPITQTVAYVGKPGEVIEEKEESVPAPQKTEEKPVS
                     VQIEEKKATIDLETAGERIKASPLARRLASEYGLDLSKIQGTGPGGRIVKEDIEKAYQ
                     QKEIIVPQKEEKPKVEPVVMVKETLIPGEGLPKEVIPLTRMRKIIAERMHSSMQNKPH
                     FYLQMEVLADELVKLRGRMLPLIEKTTGLRISYNDILIKIVAQSLEKFPTINAYFLDN
                     GIQMNPAVNIGVAVALEDGLIVPVVKNANLKGLSQIVVETNDLSKRARESKLLPEEIT
                     GGTFTISNLGMYGVDSFNAIINAPESAILACGAIKKRPYFDGENIVVASLMNLTLSGD
                     HQIVDGAVAAQFMQYLKNLIEEPLGLIV"
     gene            complement(8811..>8998)
                     /locus_tag="PK171_00845"
     CDS             complement(8811..>8998)
                     /locus_tag="PK171_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04783.1"
                     /translation="GIGEWGYLQENQSIPINHTDGIIALDGERELPVRSGEKWTVLLD
                     RNGPVKANINQIMEKVR"
BASE COUNT         2793 a         1973 c         1482 g         2750 t
ORIGIN      
        1 tcatagatgg gttttcagga ttggagtgtt aatttgaata tttctttctc atataaaata
       61 tgatgaaggc cattaaaccg atacctaata aataatcacc gatactgatt acaaaggggc
      121 gacgcataat aagcgagtaa taaggaagag tatcacccaa aaaaggaagt cgagtagaag
      181 tattgaggag ggtataatca agataaagtc cattttccaa atgattcgct aaatcatgaa
      241 gttgaagccg ctgaaccatt ggtagccaaa ccggcatcat ccctcggtta aaaagaataa
      301 ctaggatatt gagaaatgcc ccgaccgaga caattttcat tccataggac ttataactaa
      361 aaagggtccc aataaaaaca ataatcatac caataatatt gaaccattga aaagaccatt
      421 cggagaaata cgggccaata aatttcacca aaaaacccaa aacgatgagc cataaataag
      481 gaatgttcgc ttgttttaga ttttggaagt ttccgcgact gatataacct ataacaatac
      541 caattaaaac aaaatcagcc agcaacctca aagccctcct gcaaaatctc tttaagtgct
      601 tttatgacct taaaatcata atattcgttt ccttcatttt ccattataat taaagcttct
      661 tctactgaat acgctttacg ataaggccgg tcggaagtta aagcttcaaa aacatcagcc
      721 actgctaata taagggattc aacaggaagg tcggtctgct tctttccatc cggatagcca
      781 cttccattac atctctcatg gtgagatcga attaaaccgg cgatttcctt taaaaagtcg
      841 actttggaaa ctatatcaaa acctataact ggatgctgtt taacaatgat atactctcct
      901 tcagtaagag gacctggttt tcttaaaatc gcgtcttcaa tcccaatttt tccaacatca
      961 tgaagcaagg ctgcatattc aatcatttct tgctttttat cggaaaggtt gagcttttct
     1021 gcaattgcca aagaaatttt tgctacccga gccgaatggc cttgagtata tgggtcttta
     1081 gcatccaacg cagcagtgag agctcgtaat aattcaagat ggactttttt cgtttcaaca
     1141 taaagcttaa atgaatacct tgctatcatc agaggaaaaa agaataaaaa taatcccaca
     1201 taaccaatgc gcagatatat taggtaaagc aacagtgaat aagggaaaag tccaaaatat
     1261 tgcaggagga ttccattgac atcttttttc cataaaaccg gaattgatat acccgtgctt
     1321 aaagaaatga ctatgataac caaggaagca ttacttaaga aaaaaactaa tatggatatg
     1381 acaatggcta tataaaaagc aaactgatcc caagcgtaag catagccccc gaggagttga
     1441 aaaacatacc ctgaaagaaa ggctgaaatg gcaaattgag caccattaaa caggcttttt
     1501 ttccagcctg atttcatata aattaaaaca tttccaatga aagtagcaat cattaccaaa
     1561 taagggttgg ttagtaaaat aaaagcataa attatagcaa aactaacgga tattgaagta
     1621 tggggagaaa ggtgaattgg taaagcttcg gtaaccatta tcaataccag gaaggtgatg
     1681 atttgagcaa gaggaaaatg acttaaatca atggttaaaa gcgaatataa aaataaagca
     1741 atgccaaaaa taataactga ataaatataa aaatctagta gatgcttctt tttatcggac
     1801 ttcatccaaa attttaaaaa aggcgaccag ggaggcttct attgcaactt tagcttatct
     1861 ccaatggaaa ccagcaccac tggctaaaac gatggccaaa agaccaagaa gaatgctcag
     1921 cagttttttc attcgaaagc cctccctgcg atcattctcc acctttcttc gctaagtaaa
     1981 gatgggaagg ccgcttcgct ctatagaccc ctggccgcct tttcttctcc ttatagttct
     2041 atatccataa tagatggact ggtaaaaact tggcttctcg gtatttcctc ctcttcggat
     2101 tgcatttcaa tctatttttg gcttaaaatc actccttttc aatgttcaaa agagtttttt
     2161 tctaaatttt cccgatggaa ggaatgataa taattcactt tcctatttac tgcatccaat
     2221 gctgatcgtg caacggtttc atagatttca tctcgaagta aggctgtacc aatcaaagtc
     2281 ccttcggctt ttccgaagtt acgataatca accagacaaa ctgccacatc aaattcacaa
     2341 taattatgaa cggtaactcc tcgtaaggaa aacaatggag ttgcaacaat ttgattcata
     2401 caatctataa ctgcttgggc aattaacgaa caccgctctc tctggtccca ggctttgctg
     2461 ttaagagaag aacgataaac cttgtcaacg agtttgattt caacgtccgc ctgaaaatta
     2521 tcaccggatt ttttccatcc aactccccat aaaataggcc gttcctggat taccgttgtt
     2581 tcgtgaggtg gttcttccaa gtcgaaactc acaacgctaa tttttttatg atcaatttca
     2641 acaccaaaac gagccttgat aagagtttca atatccctaa ctaataattt aggccccttg
     2701 ctccgattgg ataaaacatg aatctcagtg atatttgaac cttctccgac aatttttaca
     2761 ttaataatgc cgtcaataca acttattgag tcctcgatag ctttaaaatc aatcggtacg
     2821 tccataattt ccccacctaa aggattcttt tttaaaactc tttatcaata atgttttcca
     2881 atgttcgata aatataactg gtttatatct taacaatgag aagttaatat ataggatcaa
     2941 ttaagccgta gtttccatcg tttcttttat agagaatttg aactttattt ttatctatat
     3001 ctttataaac aaaaaaagaa tggctgagaa gttccatttg taaaatggca tcttcaaccg
     3061 tcatgggacg aagtgtaaat tctttagtac gcaccaactt accgggaatt ttttcttcgc
     3121 tctcggtttg agaaagaatc tcattgattt cgattgccga gagagcttcc actgatttca
     3181 aagcacctcg gcgagccagt ttttctttat aacgtttaac ctgtctttcc aatttatcat
     3241 cgacagtttc gaaagatgtc ctccaatcgg gtccttctcc ttcagctcga agaacaatcc
     3301 cacttgctgt catggtaact tcacttttaa ttcgtcctcg aaccaaactg aaaacaacag
     3361 ttgcatcgat aagcttatgg aaaaacttat caagttttga caactttttg gtcacataat
     3421 ctctcatgag gtcgtcgaca tcgatatttt ttcctcgaac caccacttcc atcgcagcta
     3481 cctcctttta gatgagtaat aattttcaat gaaaatgtgc tccataaact ggtgtgccat
     3541 atccaggtta aacacccccg ctcctgaaca aaaggtattg gcgagcccac aggcggttcc
     3601 aaatataata gcatccttaa catcaacctg ttgtgaaagt tgagctacta ctcctccaac
     3661 taaggtatcc cccgatccaa ttgcatatcc cccacccctt aaggtattat cggctttcac
     3721 aataaaaact tcatcatgat atccaaccac taatccattt ggaccatcgg atagaataac
     3781 taagggtatc tttatctttt gaaaacaagc taccgctttt attttatcac ccaatgatag
     3841 aatagcaaat cccagtgcct cctcggcttc tttaacattt ggtttaacta aaaatggaat
     3901 tgacttcaga gccaaagaaa aaggtatacc agagctatca agcactgaat aagctttttt
     3961 ttgatgcgca atatgaatta aaactgaata caaatctctc ctgactcctc ttggtaatga
     4021 accagacagg ataagaaaat catccggttt aagttgtttc tctatgctat ggattaaatc
     4081 taaagaagat tcaaaaggaa caatagggcc actttcgtta attaccgtat gccgatgtat
     4141 tttctcttca tgaataattg tggtaagacg agtttctcca agagttggcc aagcatcaaa
     4201 aggaagacac tcttgggaca aacctctcgc caccttattc ccattttcgc ctcctaaaaa
     4261 taggaagaga tgactttcaa gccccattgt ctgaagtgct cgagcaacat ttattccctt
     4321 tccagccacc gtgacctcaa cccgagaagt tcgattcact ccatctaaac caagttcggg
     4381 taaaacgagc agtcgatcaa gagccggatt ggcacaaacc accaaaatca caaggggttc
     4441 tcctcaataa aaatttcaac ctcttcttga gttggaagag ctggaatagc tccctttttt
     4501 aaggttgtga gtgctcctac cgcattggca cgacgacctg cataacgaag tgtgtcatca
     4561 gggatggatt ttctttctct caggtcattc caaaacatat aaagtgaact gatcagtcca
     4621 gcaacaaagc catctccagc tccagtagta tcaacagcat gaaccggaaa gccttctact
     4681 cgaagtgatt tttcttggtg aacaaggata gaaccttctt tccccaaggt tataataacc
     4741 aaagcaagat tgtagcttcg ttgtaagtaa cgaattgctt cttcaacggt attggtttct
     4801 gcaataaaca tcgcttcttc ttcgtttaat tttacaacat cggatagttc cattccaagc
     4861 cgcatttttt cccgtgcttc atccaaggtt ttccataagg ctggccgtaa attcggatca
     4921 aaagatacca aataaccata tttacgagct aattgaattg ccttaaatgt cgcgctattg
     4981 gatggttcgt gagtcatagt aattgatcca aaatgaaaaa caccgcttcc agaaaatatc
     5041 aaatctataa tatccgattg gtccaaaagc atgtctgctc ctggatctcg gtaaaaaaca
     5101 aaatcccttt cgccctcttc agttaaagaa acaaaagcta aagtagtttt tgctttagtc
     5161 gttgtagtta aataattagt tttgacacca tacttgtcca aagtcattct taaaaaatca
     5221 ccaaaggcat ctttgccaat ttttccaata aaataagttt caatacctaa ctttgacaag
     5281 cccactgcta cgttggcagg agcaccccct ggtgcttttt caaagcctgg agcatccctt
     5341 aaggttacgc catttaaagt ggatacgaaa tcaattaaga gttcccctaa acatatggct
     5401 ttcttatcca atagtctcac ctcctaaaat aacaatgatc ggtgactggt gaataatggt
     5461 ggataatgtt ttttatactt ctccttcacc ctcgtctttc ctttaaagag agaaggagct
     5521 cctcaatgat taaaaaactt tccccctcgc tccatcataa aagatggtca ggggtgaggg
     5581 ggtaattatt ttcattgtca taagatacta tttagagtca tgaacgtcta tccttcaatt
     5641 ttctttatcc tgattaccaa gaaatattag aaatcctctt ataaaatggt tttaaagccg
     5701 gatacaggtc tttataaatt tgataaattt cgttataccg aatacccttt tgggaatcgg
     5761 gataaacttt atccatcact ttcaccgcct tttgacaggc ttgctccaca ttaggaaaaa
     5821 ctcccttccc aaccgctgct aataaagctg caccaaatgc cggcccttca tctactcgaa
     5881 cctggtacaa gggaacttga tatatatcgg cttgaatttt tctccacaat gggcttttgg
     5941 caccaccacc aatggccatg acttccccaa ttggaattcc catttctcga atgatctcaa
     6001 aggaatccct catgccaaag gtaactcctt ccataactgc acgcactatt tcattcttcc
     6061 gatgcttgag ggttaaacca aaaaacactc ctcgagcata gggatcggca tgaggcgtcc
     6121 tttcgccggt tagatagggc aaaaagatca gaccttcacc acccggttca acctgagccg
     6181 cttcttcggt taaaatctca taggcatctt tatcaagtag tgccgccagg tttttttcct
     6241 ccgttccaag aacatcacga agccaacgga aagatccacc agctgccaac ataacaccca
     6301 taacgtgcca cttacccgga accgcatggc aaaaggtatg aacacggcct ttcatatcaa
     6361 ttttaacttc atcactaaaa gcaaaaacca cgcccgaagt accaatactc gctgatacca
     6421 agccagactt aacaatacca gttcctatgg ctccagctgc gttatcccct cccccggcaa
     6481 caacaataac ttggtcattc attccaaata actgagcaat ttctcttttt aaataaccag
     6541 ttatctccgg tgactcaaag caatctggaa accaatttcg attgagttta agatcagaaa
     6601 tgatttcatc tgaccattgt cgacggggta catcaaataa cgaagtacct gatgcgtcag
     6661 aaacatcggt tgcgaattca ccggtcaggc aaaatcgtat atagtctttg ggaagcaaaa
     6721 ttttatcaat acgtttataa tgatctggtt cattattttt caaccaataa atttttggtg
     6781 ctgtaaaacc ggctaaaacc ggatttccgt tgatccgcat cattttttct cgaccaacga
     6841 tttgtgtgat ttcttcgcat tcctttgcag ttctttgatc gcaccacaat atagccggac
     6901 gaatcacttc tcctttatta ttgagaaaaa ccgacccatg catctgacca gtcaaaccaa
     6961 taccaacgat agcttttcga tcaatagaaa ctgaatcaag ggtttttttg atagcttgtt
     7021 gggttccctg ccaccaatca gaaggatctt gttccgccca attgggacgt ggagtataaa
     7081 ggggatattc ttctacaccc ttcgcaataa gttcaccatt ttcgtcaact aaaattgctt
     7141 tacacccagt agttcctacg tctattccta gaaaataatt cactgattga ccctcccttt
     7201 tttatttata cgattaatcc caaaggctct tcaatgagat tctttaaata ttgcatgaat
     7261 tgggctgcaa ctgccccatc tacaatctgg tgatctcccg ataaggttaa gttcattaaa
     7321 gaagctacaa caatattttc tccatcaaaa tagggacgtt ttttaattgc accacaggct
     7381 aaaatggccg attccggtgc attaataata gcgttaaaag agtcaactcc atacatgcct
     7441 aaatttgata tagtaaaggt gcctccggtt atttcttcgg gaagcaattt tgattcacga
     7501 gctctttttg ataaatcatt ggtttcaact acaatttgtg atagtccttt cagattggca
     7561 tttttaacta ctggaacaat taaaccatct tccaaggcaa cagctactcc aatgttaacg
     7621 gctggattca tctggattcc attatcgaga aagtaagcat tgatggttgg aaatttctct
     7681 aaactctggg caacaatttt tatcaaaata tcattgtagg aaattcgaag tccagtggtt
     7741 ttttcaataa gaggcaacat ccttcctctt aatttaacca attcatcagc taaaacctcc
     7801 atttgtaagt aaaaatgagg tttattttgc atactactgt gcattctttc tgcaataatt
     7861 tttctcatgc gggtaagagg aatcacttct ttgggtaatc cttcaccagg tatcaatgtt
     7921 tcttttacca taactactgg ctctactttt ggtttttctt ctttttgtgg tacaatgatt
     7981 tccttttgtt gatatgcctt ttcaatatct tctttaacga ttctccctcc cggtccggtt
     8041 ccttgaattt ttgataggtc aagaccatat tcactggcta atcgacgagc gagaggtgaa
     8101 gctttgattc gttctcctgc agtttccaaa tctatagtag cctttttttc ttctatttgg
     8161 actgataccg gtttctcttc agttttctga ggagctggaa cagattcttc tttttcttca
     8221 ataacttcac ctggtttccc aacataagcg accgtttggg taattggaat ggtttcccct
     8281 tcggcagcca taatctttaa aacaactccg ttagctgttg attcgacctc catattcact
     8341 ttatcggttg cgacttcaaa tagtgcatcg cccttctcga cctgatctcc ctctttgacc
     8401 aaccatttgt taataactcc ctcttccatg gttaatccca atttgggcat aattattttt
     8461 tctgccattg gcaatctctc ctttttaata aaaaaaataa gttaaggatt taaagaaatg
     8521 aatacaaaaa caactctatt aacgttttaa aaataaaggt accttatctt tataatcaga
     8581 aatcattata aaaactatca tcccgagtaa agatattccc agtgagaatc tgattatttc
     8641 gtctttttta attatcagac tcaaatccaa gcctatctat ctttgccaga aaaaggatat
     8701 tgactttaca ttcatttttt tattgacaat cggtgttatc aaatagagtg atttcttctc
     8761 taacttatct aagtagtttt tcttcgtaaa ccatgatagg atgatctcag ttatcgcacc
     8821 ttctccatta tctgattgat attcgctttt accggaccat ttctatccaa taggaccgtc
     8881 catttttccc ccgaacgaac cggcagttct ctttcgccat cgagagcaat tattccatca
     8941 gtatgattaa ttggtattga ttgattttcc tgaagataac cccactcacc aataccaa
//
LOCUS       DAOSOL010000016         8628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_8142_length_8628_cov_4.198414, whole genome shotgun sequence.
ACCESSION   DAOSOL010000016 DAOSOL010000000
VERSION     DAOSOL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8628
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8142_length_8628_cov_4.198414"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="ispH"
                     /locus_tag="PK171_00850"
     CDS             <1..723
                     /gene="ispH"
                     /locus_tag="PK171_00850"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOT04784.1"
                     /translation="RGARLEDEVEQIPDKVTVVTRSHGLERKTRETLLEKGVTIIDTT
                     CPRVRKVQSLASELEKKGYTMIILGDPNHAEIKALISELNTKPLVLGKDSTDWENKLN
                     TLPENQPLAVIEQTTFPQQQYLDFCRLLEEKNLTDRCLIYNTLCLETEYRQNELRSHL
                     QTGKIDVVIILGGKHSSNTRGLYLIANEQAPRTIWIEDPNEVEKTWFQPNQTILIVSG
                     ASTPDCAVKELMKKLNKGKVYE"
     gene            716..1624
                     /gene="der"
                     /locus_tag="PK171_00855"
     CDS             716..1624
                     /gene="der"
                     /locus_tag="PK171_00855"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT04785.1"
                     /translation="MNKEAPLVVIVGKTNVGKSTLFNRLSGRRITIVDSTPGVTRDLI
                     ENIIEIDGVLVRLVDTAGIELTVSPDDPLQTEAEKRVWEVINKADYILLILDGKEGMT
                     SVDHELISRLRRVKDTIIPIVNKREGMTHLGLLSDFYALGFDDVVQISAVHGDGIPQL
                     KSILFDRLKSDTESEDAQVVEHHDFRIAIVGKPNVGKSTLFNMLLESDRSIVSTIPGT
                     TRDPLESSLVSGGYGRYQLVDTAGLARKSRIKEDIAFYATVRTMEKIAETNLCVLVLD
                     VQEGISQQDRHIANQIYQQKKCCLFS"
     gene            1630..2064
                     /locus_tag="PK171_00860"
     CDS             1630..2064
                     /locus_tag="PK171_00860"
                     /inference="COORDINATES: protein motif:HMM:NF026064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04786.1"
                     /translation="MDIIPERLTRKKIEETKKWIWNELNFLDYATVLIGSATDRKLKP
                     HFLAQFATIASRYYQRLKTSFINREIKEKINDLNISMGKGRLLRVYYGYQELTAPPRF
                     SFLTNFKGNDRELERSIHSIEKIIRQEGDWEGVPLTIELKNK"
     gene            1994..2968
                     /locus_tag="PK171_00865"
     CDS             1994..2968
                     /locus_tag="PK171_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT04787.1"
                     /translation="MKKSFVRKVIGKVYPSPSSLKINNELINEFHEYLEFERHYSEHT
                     IENYLRVVNQFNNYLMKTNKRSLVEVGTSDVAGFMAFLKTISHLMRVSQLNRLSALKT
                     FYRYLRKRGKIQKNPCDGIGGLREEKSLPTFFSQQEINRFLTYLEDRFQKKPNFLQAR
                     NRALFEILYSSGLRVGEVVRLKTIDLNFRQKIVKVRGKGGKERIVPFNDKALQAVEDY
                     LAFRRPKGPVKELFLNYLQQPLTTRGVRVILNKILAEIGMVKKASPHTFRHSFASHFL
                     SGGADMRIVQEALGHSSLSTTQIYTHLNWDNMKKVYENTHPRSHKKED"
     gene            2969..3502
                     /gene="hslV"
                     /locus_tag="PK171_00870"
     CDS             2969..3502
                     /gene="hslV"
                     /locus_tag="PK171_00870"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOT04788.1"
                     /translation="MKIISTTVLGVLRNGKAAMACDGQVTMNESVLKRGAKKIRTLYQ
                     GNVLAGFAGAGADCLTLLDRFEEKLNSYSGNLSRAAIELAKMWRTDKVLRHLEALLLA
                     MDLEHVLIISGKGDVIEPDESIAAIGSGGNYALSAAKALYRNTDMDSEAIARRSLEIA
                     AEICIYTNDHIHLETLG"
     gene            3545..4942
                     /gene="hslU"
                     /locus_tag="PK171_00875"
     CDS             3545..4942
                     /gene="hslU"
                     /locus_tag="PK171_00875"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOT04789.1"
                     /translation="MEQIDDFLTPEEIVRELDKYIIGQEDAKKSVAIALRNRMRRRML
                     PQEIAEEITPKNIIMIGPTGVGKTEIARRIAKLTGAPFMKVEATKFTEVGYVGRDVES
                     IIRDLAELSLRMVKSEKISAQEGKTRWVVEERLLDLLFNKRITPEDFDEPSPSGTSTR
                     VDVVWPTPSSSAERTREKIREKLHQGELEDREIEIEIEESSIPVEVVNVGGLDNMGID
                     FSGLFGGIFPARKKKKRVKVKDAREILFNQEAQKLLDMDDIKSEAIRRVEESGIVFID
                     EIDKIASPGGRDTHGPDVSRGGVQRDLLPIVEGSTVVTKHGPVRTNHILFIAAGAFSH
                     TKPSDLLPELQGRFPIRVELSALSEEDLYRIVVEPYNSLVKQYTAMLSTEEVELQFTD
                     DGLREIAHISSILNQKMENIGARRLHTVVERVLQDISFRAPNLKQKVVVIDRAYIQDR
                     LKNVLQDEDVTRYIL"
     gene            5121..5792
                     /gene="rpsB"
                     /locus_tag="PK171_00880"
                     /pseudo
     CDS             5121..5792
                     /gene="rpsB"
                     /locus_tag="PK171_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236109.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            5996..6592
                     /gene="tsf"
                     /locus_tag="PK171_00885"
     CDS             5996..6592
                     /gene="tsf"
                     /locus_tag="PK171_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOT04790.1"
                     /translation="MSYDAKVVYELRNRSGAGVMECKKALEETKGDIEKACELLRKKG
                     IETAAKKQNRSTEQGLIGSYVHTDGKIGVLVEVNCETDFVARTEEFISFVKELTLQIA
                     AQAPRWVAIEDVPEDIRKEEEKIYQAQLSESGKPEAVQKKIIEGKLNKFYQDNCLLEQ
                     EYIRDNDKKIKELLVETIAKVGENIVIKRFVRLKLGEE"
     gene            6594..7343
                     /gene="pyrH"
                     /locus_tag="PK171_00890"
     CDS             6594..7343
                     /gene="pyrH"
                     /locus_tag="PK171_00890"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOT04791.1"
                     /translation="MVEKVKPLYRRILLKLSGEALMGSLSSGIDNRVLEYFTQEIKQI
                     HRLQVEIAIVVGGGNIFRGRTAGDRGISRVTADNMGMLATVINALALQDFLENADIPT
                     RVQTAIEMRAVAEPYIRRRAIRHLEKGRVVIMAAGTGNPYFTTDTAAALRAAEIKADA
                     VIKATKVDGVYESDPVANRNAVKYETISYLDVLNLGLKVMDSTAVSLCMDNNIPIIVF
                     NFNQTGNLLRVIIGDKIGTIISQEGGKWPNN"
     gene            7328..7894
                     /gene="frr"
                     /locus_tag="PK171_00895"
     CDS             7328..7894
                     /gene="frr"
                     /locus_tag="PK171_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT04792.1"
                     /translation="MAEQLKDLLKDVEKRMKAAITVLREELSRVKTGRATPALIENVI
                     IDYYGSMVELKTIASINTPDAKTIIVQPWDKNALQPIEKGIWKSDLGFNPVVDANIIR
                     INVPPLTEERRKEIAKFTKKTAEEAKVAIRNLRREANEGIKKLEKDGVISEDESKKSV
                     AEAQKMTDEFINEIDGIWEKKEKEIMSI"
     gene            7858..8586
                     /gene="uppS"
                     /locus_tag="PK171_00900"
     CDS             7858..8586
                     /gene="uppS"
                     /locus_tag="PK171_00900"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOT04793.1"
                     /translation="MGEKREGNHEYLDFGIHHVAIIMDGNGRWAQARGKPRLEGHRQG
                     VETVRMVVKETWDEKIPFLTLYSFSTENWRRPINEVQGLMQIFIEAIDRYADELHQNQ
                     VKVRFIGKKEGFPGHLTERMENLEKLTGQNQGLTLNLALNYGGRDEIIRAFQKFLQSS
                     LNQEREIGEDSFRQFLDTENQPDPDLLIRTGGEKRLSNYLLWQLAYSELYFTEILWPD
                     FSYQDYHQALADFISRKRRFGGLG"
BASE COUNT         2783 a         1477 c         2008 g         2360 t
ORIGIN      
        1 aggggagctc gattagaaga tgaggtggaa cagatacctg ataaggtaac tgtggttacc
       61 cgttcccacg gtttggagcg taaaacccgg gaaacgcttc tggaaaaagg agttaccata
      121 attgatacca cctgtcctcg agttcgaaag gtacagtcat tagccagtga attagaaaag
      181 aagggatata ccatgattat tctcggagat cccaaccacg ccgaaattaa agccttaatc
      241 agtgagctaa acaccaaacc tttggtatta ggtaaggatt caaccgattg ggaaaataaa
      301 ctcaatactt taccagaaaa tcaacccttg gcggttatcg agcaaactac ctttcctcag
      361 caacagtacc tggatttttg tcgattactg gaagaaaaaa atttaaccga tcggtgtctg
      421 atttataata ctctctgtct agaaaccgaa tatcggcaaa atgaattaag atctcatctc
      481 caaactggga aaatagatgt ggttataatt ttaggaggga aacatagttc caatactcgc
      541 ggtctttatt tgatagcgaa tgaacaagct ccacgaacca tttggatcga ggatcctaac
      601 gaagtggaaa agacctggtt ccaaccgaac caaactatat taattgtcag tggggcttca
      661 actcccgatt gtgcagtaaa agagttgatg aaaaaattga ataagggaaa agtctatgaa
      721 taaagaagca ccattagttg ttattgtagg aaagaccaac gttggcaagt caaccctttt
      781 caaccgtctc tcgggacgaa ggattaccat tgttgactca actccaggtg taaccagaga
      841 tcttattgaa aatatcattg agatcgatgg agttctggtt cgattggtcg atactgctgg
      901 tattgaatta acggtatcac cagatgatcc tctgcaaacc gaagcagaaa aaagagtgtg
      961 ggaagtcatc aataaagctg actacatttt gttgattctt gatggaaagg aaggaatgac
     1021 cagcgttgac catgagttaa taagtcgttt gcgccgagtg aaggatacta ttattccgat
     1081 cgtgaataaa agggaaggga tgacccattt aggattacta tcggatttct atgcattagg
     1141 gtttgatgat gtcgtgcaga tatcggcagt acatggggac gggattcctc aattaaaatc
     1201 catccttttt gaccgattga aaagtgatac tgagtcggaa gatgctcaag ttgtcgagca
     1261 tcatgatttt cgtattgcca ttgttggtaa acccaatgtc ggaaaatcaa ccttgtttaa
     1321 tatgttgttg gaaagcgacc gatctatagt gagtaccatt ccaggaacaa ctcgagatcc
     1381 actggaatca tcgctggtta gcggagggta cggacggtat caattggtgg atactgccgg
     1441 attggctcgc aaatcccgta tcaaggaaga tatcgccttt tatgccacag tacggactat
     1501 ggaaaaaatt gctgaaacca atctttgtgt gttggtcctt gatgttcaag aagggattag
     1561 ccagcaggat aggcacattg ccaatcaaat ttatcagcaa aaaaagtgct gtctattttc
     1621 ctgaataaga tggatataat tccagagcga ttgactcgaa aaaaaattga ggaaaccaaa
     1681 aagtggattt ggaatgaatt aaattttctc gattatgcta cggttttgat tgggtcggca
     1741 accgaccgaa aactgaagcc tcatttttta gctcaatttg caaccattgc gagccgctat
     1801 tatcaacggc ttaaaaccag ctttatcaat cgagagatta aggaaaaaat taacgattta
     1861 aatatcagta tgggaaaagg aaggcttctc cgtgtctatt atggatatca agagctcact
     1921 gctcctcctc gtttttcgtt tttaactaat tttaaaggaa acgatcgaga gctggagaga
     1981 agtattcata gtattgaaaa aatcattcgt caggaaggtg attgggaagg tgtacccctc
     2041 accatcgagc ttaaaaataa ataatgaatt aattaacgag tttcatgaat atctggagtt
     2101 cgagcgccat tactcagaac atactattga aaattatctt cgagtggtga atcaatttaa
     2161 taattattta atgaaaacca ataaaaggag tctagtcgag gttggtactt cagatgtagc
     2221 cggatttatg gctttcctaa aaactatttc ccatttgatg agagtttctc agctgaaccg
     2281 attgtcggct ttgaaaacct tttatcgata tttacgaaaa agaggaaaaa ttcaaaaaaa
     2341 tccttgcgat ggtatcggag ggctcaggga agaaaaaagt cttcccacct ttttttctca
     2401 acaggaaatc aatcgatttt tgacttattt ggaagatcgt tttcaaaaaa aaccaaattt
     2461 tcttcaggct cgcaatcgag cactttttga aattctgtat tcatctggtt tgcgagttgg
     2521 agaagtagtt cggcttaaaa ccatcgattt gaattttcgg cagaaaatcg ttaaggtgcg
     2581 ggggaaaggt ggaaaagaaa gaatcgtccc ctttaacgat aaagctcttc aggcagtcga
     2641 agattatctg gcttttcgtc gaccgaaagg accagttaag gaactttttt taaattatct
     2701 ccaacaacca ttaacgactc gtggtgtacg ggttatttta aataaaatct tagcagagat
     2761 tgggatggta aaaaaagcct ctcctcatac ttttcgtcat agttttgcca gtcattttct
     2821 ttcaggaggg gctgatatga ggattgttca ggaggcattg ggacactcca gtctttctac
     2881 cacccaaatt tacacccatt taaattggga caatatgaaa aaagtttatg aaaatactca
     2941 cccccgatcc cataaaaagg aggattaaat gaagatcata tcaacaacag ttcttggcgt
     3001 tttacgaaat ggaaaagcag caatggcttg tgacggacag gtaaccatga atgaatcagt
     3061 attaaaaaga ggagctaaaa aaatacggac cttatatcaa gggaatgttt tagccggttt
     3121 tgcaggagct ggtgcagatt gtctcacctt gctggatcgt tttgaagaga aattgaattc
     3181 ttatagtgga aatctgtctc gggcagccat tgagttggct aaaatgtgga ggactgataa
     3241 agttctcagg catcttgaag ctttattgct ggctatggat ttagaacatg tattgattat
     3301 ttcagggaaa ggagacgtaa tcgaacctga cgaatcaata gcagctattg gctcgggagg
     3361 caattatgct ttgtctgctg ccaaagcatt atatcgaaat actgatatgg attcggaggc
     3421 gatcgctcgt cgatccttgg aaattgctgc agaaatctgt atttatacca acgatcacat
     3481 tcatttggaa actttggggt aaaccagggg gtaagatgct tctaagagaa aggaagagat
     3541 tgacatggaa cagattgatg attttttaac cccggaagaa attgtccgag aattagataa
     3601 atatattatt ggtcaggaag acgctaaaaa atcggttgcg attgcccttc gtaatcggat
     3661 gcgacgaagg atgcttcctc aagaaatagc tgaggagatt actccaaaaa atatcatcat
     3721 gatcggtcca accggagttg gtaaaactga gattgcccgt cggattgcga aactgaccgg
     3781 tgcgcctttt atgaaggtag aggcaactaa attcactgaa gttggttatg tgggaagaga
     3841 tgttgaatca attattcgtg atctggctga gttatcactg cgaatggtaa aaagtgaaaa
     3901 aataagcgct caagaaggaa aaaccagatg ggttgtggaa gaacgcttgt tggatcttct
     3961 ttttaataaa agaattacac cagaagattt tgacgaacct tctccttctg gaaccagtac
     4021 tcgggttgat gtggtatggc cgacgccatc ttcttcagca gaacggacac gagaaaaaat
     4081 tcgagaaaaa ttacaccagg gtgaattaga agaccgggaa atcgaaattg agattgaaga
     4141 gagctcaata ccggtagaag tggttaatgt tggtgggcta gataatatgg gtattgactt
     4201 ttctggtctt tttggaggca tttttcctgc ccgtaagaag aagaaacggg ttaaagtcaa
     4261 agatgctcgg gaaattcttt ttaatcaaga agctcagaag ttattggata tggatgatat
     4321 taaaagtgaa gccatccgac gagtagagga aagtggaatt gtatttatcg atgagattga
     4381 caaaatcgcc tcaccagggg ggagggacac ccatggtcct gatgtttccc gagggggagt
     4441 tcaacgtgac ctattaccga tcgtagaagg ctcaacagtg gttaccaaac atgggccggt
     4501 tcgtaccaat catattcttt ttattgctgc cggagccttt tcccatacta aaccatcaga
     4561 tttacttcct gaattacaag gtcggtttcc catacgagtt gaactttcag cgcttagcga
     4621 ggaagacctt tatcgaatcg ttgttgagcc ttacaattct ttggtgaagc aatatacagc
     4681 aatgttatca accgaagagg ttgaattaca gtttaccgat gacgggctaa gagaaattgc
     4741 gcatatatcc tcaattctaa atcaaaaaat ggagaatatc ggtgcgcgtc gcttgcacac
     4801 ggtggtagag agagttctcc aggatatctc ttttagagca ccgaacttaa agcaaaaagt
     4861 ggtggttatt gatcgagctt atattcagga ccgattgaaa aatgttttac aagatgaaga
     4921 tgttacccgt tatattttgt aatttccttt atagataaat tggatttatg gttgaaatat
     4981 ttttcccttt catgatatac tgtgcccgat ttatgcatag cataagaata aattctcatg
     5041 ccggacaaga attccggtgc tctccaaaaa ggagataaac catcggttcg gcagaggaaa
     5101 aaccggaagg agaattattt atgatcgtta ctatgaagca gttgttggaa gctggggttc
     5161 attttggtca tcaaacccgc cgttggaatc caaaaatgaa accctatatt tttaccgaaa
     5221 gaaataatat ctatattatt gatttacaaa aaaccgttgg cttagctgag aaggcttact
     5281 atttcgttcg tgatttagcg aaggagagtg gaacggttct ttttgtcggg accaagaaac
     5341 aggcgcagga agccatagag caagaagcga accgttgtgg gatgtattat gtgaatcagc
     5401 gatggctggg gggtatgctt actaattttg taaccattcg gcagagcata gataaattgg
     5461 aacacattga gaaattggaa gaaaacggta tcttaaaccg acttccaaaa aaagaaatta
     5521 tgcaaatctt aaaaaccaag actcgattag aaaaatacct tactggaatc cgaaagatgg
     5581 ccagggttcc cgattgtgtg tttgttgtcg accccaggag agagcgaaac gccgttttag
     5641 aagctcgtcg tattgggatt ccaatcgttg gtattgtaga taccaattgt gatcctgatg
     5701 aagttgattt tgtgatacca ggtaatgatg atgccatacg agctattcgt ctattctcat
     5761 ccatcatagc cgatgccgtg atggagggta aacagtatgc aaccgagggt cgtgaagaag
     5821 ttactactga aggagaagct gtccctgaaa ccgaggtggc ggtttctgat gaagtatcca
     5881 ccgacaatga agaagaactt cctctttcga ttttagaaga gctcaaagat cctgaactgg
     5941 aagaaattga aagtgacgat gaaaatctca ccgaagaagc aaaggaggaa caaccatgag
     6001 ctatgatgca aaagtagttt atgagcttag aaaccgaagt ggtgcaggag ttatggaatg
     6061 taaaaaagcc ttagaggaaa ccaagggaga tatagaaaaa gcctgtgagc ttcttcgtaa
     6121 gaagggaatt gaaacagcgg ccaagaagca aaaccggtca accgaacagg gcctcattgg
     6181 ttcctacgtt catactgacg gaaagatagg agtattggta gaagtcaatt gtgaaactga
     6241 tttcgtagcc cgtaccgaag aatttatcag ctttgtcaaa gaattaactc ttcaaattgc
     6301 agctcaagct cctcgctggg ttgcgattga agatgtgcct gaagatattc gtaaggaaga
     6361 agaaaagatt tatcaagctc agctcagtga gagcggtaaa cctgaagcgg tccaaaagaa
     6421 aattattgaa gggaagttga ataagtttta tcaagacaat tgtctgctcg aacaggaata
     6481 tattcgagat aatgataaaa aaattaaaga attattagtg gaaactattg ccaaagtggg
     6541 cgagaatatt gttattaaac gtttcgtgcg tttaaaactt ggtgaagaat agtatggttg
     6601 aaaaggtaaa gccgctttat cgacggattt tattaaaatt atccggagaa gcgctgatgg
     6661 ggtcactctc ttcgggcatt gataaccgag ttttagagta ttttacccaa gagattaagc
     6721 aaatccaccg attacaggta gaaatagcta tagtcgttgg tggtggaaat atatttcgag
     6781 gacgtactgc cggagatcgg ggaataagcc gggtgaccgc tgataatatg gggatgttag
     6841 cgacggttat taatgcgtta gctcttcaag attttcttga aaatgctgat atccccactc
     6901 gagtccaaac agctattgaa atgagagcgg tggccgaacc ttatattcga agacgtgcca
     6961 tccgccactt ggaaaaagga cgggtggtca ttatggcagc tggaacggga aatccatatt
     7021 ttacaaccga tactgcagct gctctccgag ccgccgagat caaagccgat gctgttatta
     7081 aagcaactaa agttgatggt gtatacgaat cagatccggt agcgaaccgg aatgcggtca
     7141 aatatgagac tatcagttac cttgatgtac tgaatctagg actgaaggtt atggattcga
     7201 ccgcagtttc tttgtgcatg gataataata ttccgatcat tgtttttaac tttaaccaaa
     7261 ctggcaatct tttacgagta attataggtg ataaaattgg aactattatt tctcaggagg
     7321 gaggaaaatg gccgaacaat taaaggattt attgaaagat gttgagaaaa gaatgaaagc
     7381 ggctattact gttttacgag aagaattgtc ccgtgtgaaa actggacgag ccactccggc
     7441 tctcattgag aatgtgatta ttgattacta tggaagtatg gttgaattaa aaaccatagc
     7501 atcgatcaat acacccgatg caaagaccat tattgttcaa ccctgggata aaaatgccct
     7561 ccaaccgatt gaaaagggta tttggaaatc cgatttgggt tttaatccag tggttgatgc
     7621 taacattatt cgaatcaatg ttcctccatt aaccgaagaa aggcgaaagg agattgccaa
     7681 atttaccaag aaaacagccg aagaagccaa ggttgcaatc cgtaatttaa gacgagaagc
     7741 taatgaagga ataaaaaagt tggaaaaaga tggagtaata tctgaggatg aaagtaaaaa
     7801 atcagttgcg gaagctcaga aaatgactga tgaatttatc aatgaaatag atggaatatg
     7861 ggagaaaaaa gagaaggaaa tcatgagtat ttagattttg gaatccatca tgttgccatt
     7921 attatggatg ggaatggtcg ttgggcacaa gctcgaggga aaccgagatt agaaggtcat
     7981 cggcagggag ttgagaccgt tcggatggtg gttaaagaaa cctgggatga gaaaatccct
     8041 tttctcaccc tctattcttt ttctaccgag aattggcgac gaccaataaa tgaagtacaa
     8101 ggactgatgc aaatctttat cgaggcaata gatcgttatg ctgatgaatt gcatcagaat
     8161 caggtaaaag ttcgatttat agggaaaaaa gaaggttttc ctggtcattt aactgaacga
     8221 atggagaacc tggagaaatt gaccggacaa aatcagggtt taactttgaa tttagctcta
     8281 aattatggag ggagggacga gattataaga gcgtttcaga aatttctcca aagttcattg
     8341 aatcaggaaa gagaaattgg tgaagattcc tttcgtcaat ttctcgatac cgagaatcaa
     8401 cctgatcccg atctcttaat ccgaactggt ggagaaaagc gcttaagtaa ttacttactc
     8461 tggcaattgg cttatagcga gctctatttt accgagattc tatggcctga tttttcgtat
     8521 caagactatc atcaagcact tgctgatttt atcagccgaa aaagaagatt tggggggtta
     8581 ggctaagagg tgaccaaatt ttcccagccg acggaattta aactgagg
//
LOCUS       DAOSOL010000017         8627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_8144_length_8627_cov_4.226785, whole genome shotgun sequence.
ACCESSION   DAOSOL010000017 DAOSOL010000000
VERSION     DAOSOL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8144_length_8627_cov_4.226785"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK171_00905"
     CDS             complement(<1..367)
                     /locus_tag="PK171_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT04794.1"
                     /translation="MSEEKILGIGNLLKSQREKLGLSLEDIEQQTFINKRYLKAIEEE
                     QWESLPGFSHAFGYVKNYGRVLNLDQETLKSSFHQSYPNLSKNEPSPRSKYREYTPDK
                     ADLFKKILLFLIIAAAILTS"
     gene            complement(364..2550)
                     /locus_tag="PK171_00910"
     CDS             complement(364..2550)
                     /locus_tag="PK171_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOT04795.1"
                     /translation="MLSKKNKILIDTFLIIFGAYALLALLSYDVGILGEYIKNFLLYV
                     FGIGAYIIPFYLFYLSIEIYTFEHPGRFRTWSRIAGITLWFMIFLTVSQWQADQNNFA
                     LQSGEFGGWFGKALFSSLIYYLGSSGLFLVLLCLSFFSTFIIGEGRWIRALLSTIKKG
                     LSLSHRHSNHYPEEAQPLSPKKETKTRLFPKKTQSKAAPTLESKIITKESLISEGKNL
                     EDDVFRLEELDIEPPAEPLPQIVNTPPAPEKTGGAVSKAKKKRGKEWMLPSMNILNEY
                     PNRGDRVSREEITLGIKKLEQTFLEFNVPGKVVNVRIGPTITRYEFQPAPGIKVNKIV
                     ALSNDIALAFAAAAVRIEAPIPGRSAVGIEIPNESKEIVSMKELLETTNFIKDTSPLS
                     IVVGKDVIGRPLICDLRDMPHLLIAGATGSGKSVCINVILASLLYKADPNQVRIAMVD
                     PKRVELSIYADLPHLCVPVISDVRWVVKFLKWLCREMDTRYKMLSDLSTRNIEEYNKT
                     VEEYERLPYVVVIIDELADLMMTAPSDVESYICRLAQMARAVGIHLVVATQRPSVDVI
                     TGLIKANFPSRISFAVSSQADSKTILDGSGAEKLLGNGDMLFSPVGVNKSIRGQGAYI
                     STAETKKIVEHWLNQGGEIYYAMDFVLKEEVADTSDDEDELYREAVSVVIENGKASTS
                     LLQRRLRIGFNRAARLIERMESEGLVGPYEGSKPRKVIANRGDDIL"
     gene            complement(2570..4723)
                     /locus_tag="PK171_00915"
     CDS             complement(2570..4723)
                     /locus_tag="PK171_00915"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOT04796.1"
                     /translation="MKINRTEIEFGENNCIIETGKVAKQAGGAVMVRYGDTEVLVTAT
                     ASEEPREDIDFTPLLVDYEERFYAAGKIPGGFIKREGRPRSDAILNGRLIDRSIRPLF
                     PEFYRNDIQVVTTVLSVDEKNTPEVLGLLGASLALGISEIPFEGPIGACRIGLLDKDN
                     FIINPSLEQLVSSRLDMVIAGSEEGITMIEAGAKEVSEADITQALRIGYEFILKTIQT
                     QKAIISQWGKTKKEFEIPAYFSEIEDWIRNNYHLKIADKIAVFDKSERQKTINLLYKQ
                     AVEEATPTFENLQGFALLWEKIVKENIQDLLFKSGLRQDGRRPNEIRPIECEVGILPR
                     THGSALFTRGQTQAMAITTLGATSEGQKVDGLQDEEARRFMLHYNFPPFSTGEVRPMR
                     GPGRREIGHGALAERALEYFIPPESEFPYAIRIVSEILESNGSSSMATVCGGSLSLMD
                     AGVPIRSACAGLSIGLMRNQETNILLRDILGSEDHYGEMDFKVAGSRQGITAIQLDVK
                     NRGLSWELLSQALEEAKSGRNHILEIMDQAIHAPREILSPYAPKIGIIEINPDRIGSV
                     IGPGGKIIKKIVDETGAIIDIQDDGKIIIFSRNEEGKVKAVEMIKNITQEVEIGKTYL
                     GRVTKTTDFGAFVEIFPGREGLVHISQLARERVRKTEDVVKTGDDILVKVIGIDSFGK
                     VSLSHKDTLSDSPVPSKDIQRSREKPRFHSKRDKD"
     gene            complement(4734..5003)
                     /gene="rpsO"
                     /locus_tag="PK171_00920"
     CDS             complement(4734..5003)
                     /gene="rpsO"
                     /locus_tag="PK171_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOT04797.1"
                     /translation="MALTKEKKNELIEQFKNHDGDTGSPEVQIALLTERIRELTEHLK
                     IHTKDFHSRRGLFMMVGQRRRLLNYLKKYHVDRYYSILEKLSLRK"
     gene            complement(5040..5978)
                     /gene="ribF"
                     /locus_tag="PK171_00925"
     CDS             complement(5040..5978)
                     /gene="ribF"
                     /locus_tag="PK171_00925"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HOT04798.1"
                     /translation="MLSKGFPKNYPESVIALGFFDGVHLGHQAVLNQAATIATKKKKP
                     LRIITFYPHPRNSIGHSHTIKYITTYIEKYQRLQSFYPGCEVYFLRFNRQLRHTPPER
                     FLDLINHWFKPIAVITGENFRFGYRTQGDQLFLQNFFKKRGVETAIIPSISLDGAVLS
                     STMIRQLIEKGDIKKVNMVLGYPFSIRGRVVKGKQIGSQIGFPTANLFPVSSKIMPPH
                     GVYFGYMSWKRVEYPALIYIGTKPTVSQNQRRVVEVHIAHHIRQSLYQERITVQLQQF
                     WRLEKHFPTIEGLREQITVDQAAFNAYLQHTQGMIN"
     gene            complement(5968..6873)
                     /gene="truB"
                     /locus_tag="PK171_00930"
     CDS             complement(5968..6873)
                     /gene="truB"
                     /locus_tag="PK171_00930"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT04799.1"
                     /translation="MIGGIIPVYKPVGIVSHRVVETIRKVLGVKVGHAGTLDPFARGL
                     LLICWGKATRFTSFFQDLPKTYRAWIRFGISTDSFDVFGQINNFSLQTFTTDNIEKAI
                     MPFQGTRHQVIPLFSATKYQGHRLYKYARQGKSVPELKKEITIHQLQIINIQSGSFPQ
                     AEILLSCSSGTYIRSIAHEIGEQLGGGGYVYSLRRENIGNFSWIDSMVVSDVSHQPEI
                     LIQKSIPIDQALYWIPSVLVSSEEGKKLLQGNPINWPESLPLQMNPWVKIYTQNLFIG
                     LAHINQEKRLLHPKIVVEERSRYVI"
     gene            complement(6878..7882)
                     /locus_tag="PK171_00935"
     CDS             complement(6878..7882)
                     /locus_tag="PK171_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOT04800.1"
                     /translation="MEIQHLLNSLPQKTPIVIASHLNIDGDGLGSMISLYLLLIQKKF
                     YPIMVYDDTIPYFYHFLPNLKDILPLTHPRLKQISENTVFFAVDCSNTQRLGRLNDIR
                     EKCSLVVNIDHHPDNSSYGTINYVDPHCPSTTILIYQLAKKSEVELDSSLSMSILTGL
                     ITDTGGFQYVELNQQLLSILEDLINQGASVATIMRYAFKCRRYSALKLLGIALNHFVY
                     DYQYHFGITYLLQSDFELCGASEEDAEGIVDYGLYIPGSLISVFIREVDNHSFKVSLR
                     SQDETNILPIAHAFGGGGHLKAAGFKIQGDFKTVYHNLKEHIQGYLSSQSGTPHSLSV
                     "
     gene            complement(7885..8238)
                     /gene="rbfA"
                     /locus_tag="PK171_00940"
     CDS             complement(7885..8238)
                     /gene="rbfA"
                     /locus_tag="PK171_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT04801.1"
                     /translation="MKSYRSQRLAELIKREIATMVLSNAADPRIKRLTITHVNITPDL
                     KIARVYVSFPGNKNEVEESFHALLKASRFIRSHISQNIRIRYMPEIEFIYDTSLEKAY
                     QVIDLINQIGRKSGS"
     gene            complement(8276..8557)
                     /locus_tag="PK171_00945"
     CDS             complement(8276..8557)
                     /locus_tag="PK171_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOT04802.1"
                     /translation="MRIGSGQVLLSLPESFSLKDKRRIMNSIKQRLRNRFNLSVIELN
                     PDVAWNQGFIGFACVGEDEMTVRHLIDQIGRFVEDDGRYEIIQFTIQIY"
BASE COUNT         2524 a         1757 c         1680 g         2666 t
ORIGIN      
        1 gagaggttaa aattgccgca gcgataatca ggaaaaggag aattttttta aaaagatccg
       61 ctttgtctgg agtatattcg cgatacttgc ttcgaggaga aggttcattt ttagagagat
      121 tggggtaact ttgatgaaaa ctactcttta aggtttcttg gtctaagttg agcacccttc
      181 catagttttt aacatatcca aaagcgtgag aaaatccagg aagactttcc cactgttctt
      241 cttctattgc tttcagatat cgtttattga taaaggtttg ttgttcaatg tcttctaaag
      301 acaaaccaag tttttcccgc tggcttttta aaagattgcc aattcccagt attttttctt
      361 cgctcataaa atatcgtccc ctctgttggc gattactttt ctgggtttac ttccttcata
      421 aggaccaacc aaaccttcgc tttccattcg ttcaattaac cgggcagcac ggttaaaacc
      481 aatccggagt cgtctttgta aaagagaggt tgaagctttt ccattttcta tgacaactga
      541 aaccgcttct cgatagagtt catcctcatc gtcagatgta tcagcaacct cttctttaag
      601 aacaaaatcc atcgcatagt atatttcccc accttgattt aaccaatgct caacaatttt
      661 tttggtttca gcagtactga tataagctcc ctggcctcgt atagatttat tcactcccac
      721 tggggaaaac aacatgtctc catttcctaa gagcttttca gcacctgaac catcaagaat
      781 agtttttgag tcggcttgag aagacactgc aaatgaaatt cttgaaggaa aatttgcttt
      841 aatgagaccg gtaatgacat ctactgaagg tcgctgtgta gcaaccacca aatggatgcc
      901 aaccgctcga gccatttgtg ctaaacgaca gatataggat tcgacatcag aaggagcggt
      961 catcattaaa tcggccaatt catcgataat gactacaaca tagggtagtc gttcgtattc
     1021 ttccacagtt ttattatact cttcaatgtt tcgagtggag agatcggaaa gcattttata
     1081 tcgggtatcc atttcacgac agagccactt taagaacttc accacccatc ttacatcaga
     1141 tataaccggt acacaaagat ggggaagatc ggcgtaaatc gacaattcaa cccttttggg
     1201 atcgaccata gcaattcgaa cttggttggg atcggcttta tagaggaggc tggcaagtat
     1261 tacattgata cacacacttt tccccgaacc ggttgctcca gcgattaaaa ggtgaggcat
     1321 gtcacgaaga tcacaaatta atggtcttcc aatgacatct ttcccgacca caatggatag
     1381 tggcgatgta tcttttatga aattggtggt ttctaaaagt tctttcatcg atacaatttc
     1441 tttgctctca ttaggaattt caataccaac cgctgatcga ccgggtatgg gggcttcaat
     1501 tcgaactgca gcagcagcaa aagccagggc tatatcgttc gaaagagcaa cgatcttatt
     1561 cacttttatt cctggagccg gctggaattc ataccgagtt atggtgggtc caatccttac
     1621 gttaaccacc tttcccggta cgttaaactc aagaaaagtt tgttctaatt ttttaattcc
     1681 aagagtgatt tcttcccggg aaaccctatc tccccggttg ggatattcat ttaaaatatt
     1741 cattgagggg agcatccatt ccttccctct cttttttttc gctttagaaa ctgctcctcc
     1801 agttttctct ggtgctggag gggtgttaac gatttgaggg agtggttcag ctggaggctc
     1861 aatatccaat tcttctaatc taaagacgtc atcctcaagg ttttttccct cagatataag
     1921 ggattcttta gttattattt tcgattccaa agttggagcg gcttttgatt gggttttctt
     1981 tgggaagaga cgagttttgg tctctttttt tggggaaaga ggttgagcct cttctgggta
     2041 atgattacta tgccgatggg aaagacttaa tcctttcttg atggttgaaa gaagagctct
     2101 tatccagcgt ccttcaccaa tgatgaaggt gctaaaaaaa gaaagacaaa gaagaacgag
     2161 aaaaagacca gacgatccca agtaataaat taacgaagaa aaaagagctt ttccaaacca
     2221 acctccgaat tcacccgatt gcagggcgaa attattttga tcggcctgcc actggctaac
     2281 agtaagaaaa atcataaacc agagagtaat cccggcgatt cgactccagg ttcgaaatcg
     2341 accgggatgt tcaaaggtat agatttctat actcaggtaa aaaagataaa acgggatgat
     2401 ataggcacca ataccgaaca catataataa aaaatttttg atgtattcac caaggatgcc
     2461 tacatcatag gacagtagag caagtaaggc atatgctcca aaaatgatta aaaaagtgtc
     2521 aatgagtatc ttgttttttt ttgaaagcat aactatttac tcaatctttc taatctttat
     2581 ctcttttcga gtgaaagcga ggtttttctc tggagcgctg gatgtctttt gagggaacgg
     2641 gagagtcaga tagcgtatcc ttatggctga gagacacctt cccaaaagag tcgataccga
     2701 taactttgac taagatatca tcaccagttt taaccacgtc ttctgttttc cgaacccgtt
     2761 cccgagctaa ttgagaaata tgaacgagcc cttcccttcc tggaaaaatt tcaacgaaag
     2821 ctccaaaatc agtggttttg gtcactcgtc ccaaataggt ttttccaatt tcgacttctt
     2881 gagtaatgtt tttaatcatt tcaactgctt taactttccc ctcttcgtta cgggagaaaa
     2941 tgattatctt tccatcatct tgaatgtcta taatcgctcc agtttcgtcg acaatttttt
     3001 taataatttt tccacctggt ccgataaccg aaccgattcg gtctggatta atttcgatga
     3061 taccgatttt cggggcataa ggagaaagga tttcacgcgg tgcatgaata gcttgatcca
     3121 tgatctccag aatatgattc cgtccagatt tggcttcttc aagtgcttgg cttaaaagct
     3181 cccagctcag tcctcgattt tttacatcga gttggatagc agtgattcct tgtcggctac
     3241 cagccacctt aaaatccatc tcaccataat gatcttcaga accaagaata tctctcaata
     3301 aaatattggt ttcttgattt ctcattaagc ctatcgacag accagcgcaa gctgatcgaa
     3361 tcggtacacc agcatccatc aatgaaagac ttcctccaca aacggtcgcc atcgacgatg
     3421 agccattaga ttcaagaatt tcagaaacaa tccgtattgc ataaggaaac tccgattcag
     3481 gtgggataaa atattccagt gctctttcag ccagagctcc atgtccgatt tcccggcgtc
     3541 ctggacctct catcggtctc acttcaccag tactaaaggg tggaaaattg tagtggagca
     3601 taaaccgccg agcttcttca tcctgtagac catcaacttt ctggccttca ctggtcgctc
     3661 ctaaagtagt aatagccata gcttgggttt gccctcgagt aaaaagagct gatccgtgtg
     3721 ttcggggtaa aatccctacc tcacactcaa tcggtcgaat ttcatttgga cgcctgccat
     3781 cctgtcttaa cccactctta aagagaaggt cctggatgtt ttctttgaca attttttccc
     3841 aaagcaatgc aaacccttgg agattctcaa aggttggagt ggcttcttca actgcttgtt
     3901 tataaagcag attgatggtt ttttgccgtt cagatttatc aaaaacggca attttgtcgg
     3961 caatttttaa atgataatta tttcgaatcc aatcttcgat ttcggaaaaa taggcgggga
     4021 tttcgaattc ttttttcgtt tttccccatt gagatatgat tgctttctgg gtctgaatgg
     4081 ttttgagaat aaattcataa ccaattctca aggcttgagt gatgtctgct tccgaaactt
     4141 ctttcgctcc agcttcgatc atggtaatac cttcttctga accagcaatg accatatcca
     4201 aacggctgga aacaagttgt tccaagctgg gattgattat aaaattatct ttatccagca
     4261 agccgattcg acaagcaccg attgggcctt caaagggtat ttctgaaatt cccaaagcca
     4321 aggaagcacc taacaaacca agcacttccg gagtattttt ttcatctacc gaaaggacag
     4381 ttgtaacaac ttgaatatca tttcgataaa attcaggaaa aagtggtcgg atcgatcgat
     4441 cgatcaaacg cccgttcaga atggcatcgc ttcttggcct tccttcccgt ttaataaatc
     4501 cacctggaat ttttccagca gcatagaagc gttcttcgta atcgactaaa aggggggtaa
     4561 agtcaatatc ttcccgtggt tcttcggatg cagttgcggt tactaaaact tccgtatctc
     4621 cgtaacgaac cataaccgct ccaccagcct gtttggcgac ctttccggtt tcaataatac
     4681 aattattttc accaaactca atttcggttc tgtttatttt caacaccttc ctcttacttt
     4741 cgcaatgata acttttctaa aattgaataa taacggtcaa cgtggtactt ttttaaatag
     4801 ttcaatagtc tacggcgttg accgaccatc ataaaaagac ctcgacggga atgaaagtct
     4861 ttggtgtgga tttttaagtg ctcggttagt tcacgaatcc tttctgtcag caaagcaatc
     4921 tgaacttctg gagaaccagt atctccatcg tgatttttaa attgttctat gagctcattt
     4981 ttcttttctt tagtaagagc caattgatat tacacctccg atttctatac gttcgatgtt
     5041 tagtttatca taccctgagt atgctgtaaa tacgcgttaa aagcggcttg atctacggtt
     5101 atttgttctc tcagaccctc aatagtagga aaatgttttt ctaagcgcca aaattgctga
     5161 agttgaaccg taattctttc ttgataaaga gattgacgaa tatggtgggc aatgtgaact
     5221 tcaacaaccc tcctttgatt ttgactgacg gttggtttgg ttccaatata aatcaaagct
     5281 ggatattcta cccttttcca gctcatgtaa ccgaagtata caccatgagg aggcataatt
     5341 tttgaagaaa ccggaaaaag atttgccgtt gggaaaccaa tttgacttcc aatttgtttt
     5401 ccttttacaa ctcggccccg gatggaaaaa ggatatccca aaaccatatt tacttttttt
     5461 atatcaccct tttcaataag ttggcgaatc atggtactgg atagaactgc tccgtcaaga
     5521 gatattgatg gaatgatagc agtttcaacc cctctttttt taaaaaaatt ttgaagaaac
     5581 aattgatctc cttgggttct atacccgaaa cgaaaatttt caccagttat caccgctatt
     5641 ggtttaaacc aatgattgat cagatcgaga aaacgttcag gtggtgtatg acgaagctgt
     5701 cggttaaatc ggagaaaata aacctcacat cccgggtaga agctttgaag acgctggtat
     5761 ttctcgatat aggtggttat atattttatg gtatggctat gcccaataga atttcgtgga
     5821 tggggataaa atgtaataat tcgtaatggt ttcttttttt tggtggcaat ggttgctgcc
     5881 tggtttaaaa cggcttgatg gcctaaatga actccgtcaa aaaagccaag tgcaatgaca
     5941 gattcgggat aattttttgg aaaaccttta gataacatat cgactcctct cttcgacgac
     6001 gattttggga tgaagaagtc gtttttcttg attaatatga gccaaaccta taaaaagatt
     6061 ttgtgtataa atttttaccc aaggattcat ttgcagggga agggattcag gccaattgat
     6121 cggattcccc tgaagtaatt ttttcccttc ctcacttgaa acgaggaccg agggaatcca
     6181 gtataaggcc tgatcaatag gaatggactt ttggatcaga atttccggtt gatgagagac
     6241 atcagagact accatactat caatccagga gaaattacca atattttctc gacgtaaact
     6301 atatacatat ccacctccgc ccaactgctc tccaatttca tgagcaatac ttcgaatata
     6361 ggtaccagac gaacaagata atagaatttc tgcttgagga aatgaaccag attggatatt
     6421 gatgatttga agttggtgaa tagtaatctc ctttttcaat tcggggactg atttgccttg
     6481 gcgagcatac ttatataacc gatgcccttg gtatttagtt gctgaaaaaa gaggaattac
     6541 ttgatgtctt gttccttgaa aaggcataat agctttttca atattgtcgg ttgtaaatgt
     6601 ttgaagagag aaattattaa tttgtccgaa gacatcaaag gaatcggtag agataccaaa
     6661 acgtatccaa gcccgatagg ttttgggaag atcttgaaaa aaagaagtga aacgggttgc
     6721 tttcccccaa caaatgagaa ggagacctcg agcaaatggg tctaaagttc cggcatggcc
     6781 aacttttacc ccaagcactt tacggatggt ttctaccacc cggtgggaaa cgatccccac
     6841 tggcttatac acaggaataa ttccaccaat catgttttta tacagataaa gaatggggtg
     6901 tacccgattg ggaagaaagg tacccctgta tatgttcttt cagattatgg taaacggttt
     6961 taaaatctcc ttggatttta aaaccggctg cttttaaatg ccctcctcca ccaaaagcat
     7021 gggcaatagg aagaatgttg gtttcatctt gggaacggag gcttacttta aaactgtgat
     7081 tatcaacttc tctaataaac accgatatga gagatccagg aatatagagt ccgtaatcga
     7141 cgattccttc agcatcttct tccgaggctc cgcagagttc aaaatcgctt tggagaagat
     7201 aggttattcc aaagtggtat tgataatcgt atacaaaatg attcagggct atccctaata
     7261 attttaacgc tgaataccgt cgacatttaa aggcataacg cataatcgta gcgaccgatg
     7321 ctccttgatt gatgagatct tctaaaatac taagaagctg ctgatttaat tccacatact
     7381 gaaacccgcc agtatcggta ataagtccag tgagtattga catggagagt gatgaatcca
     7441 actccacttc acttttttta gccaattggt agataagaat tgttgttgat gggcagtgag
     7501 ggtctacata gttaatggtt ccataagagg agttatcagg atgatgatca atattgacta
     7561 ccaaggagca cttttcccga atatcgttca gtcggcccag tcgctgagta ttggaacagt
     7621 caacagcgaa aaaaactgta ttttcagaga tttgtttcag tcttggatga gttagaggga
     7681 ggatatcctt taaatttggt aaaaaatgat agaaataagg aattgtatca tcataaacca
     7741 ttattggata aaattttttt tgaataagaa gcaaataaag tgatatcatc gatcccaaac
     7801 catcaccatc gatattcaga tgagaagcga tgacgatggg agttttctgg ggtagcgaat
     7861 taagtaaatg ttgtatttcc atctttaact cccgcttttt cttcctattt gattaatgag
     7921 gtctatgacc tgataagctt tttccaggct ggtatcgtag ataaattcaa tttcgggcat
     7981 gtaccgtatt cgaatatttt gactgatatg actccgaata aatcgacttg ccttcagtaa
     8041 ggcatgaaaa ctttcttcaa cttcgttttt gttgccaggg aaagagacat aaactctggc
     8101 tatttttaaa tctggagtaa tattgacatg ggtaatagtc aatctcttta tacgtggatc
     8161 tgccgcattg gataaaacca tagtggctat ctctctttta atgagttcag ccaaacgttg
     8221 tgagcgatag ctcttcattt tcagacccct ttcaacagtt acctattttg attggtcaat
     8281 agatttgtat agtaaattga ataatttcat accgaccatc gtcttcaaca aaacgaccaa
     8341 tctgatcgat caaatgcctt accgtcattt catcttcacc aacacaggca aagccgataa
     8401 agccttggtt ccaggcaacg tcaggattta attcaataac cgagagatta aaacggtttc
     8461 tcagtctttg tttgatggaa ttcataattc ttcttttatc ttttaacgaa aaactttcag
     8521 gaagagaaag caatacttga ccggatccga ttctcattat tcttctcgta tgacataagc
     8581 ttctaaaata tctccctctt caaaatcttc aaagcgatca agtccaa
//
LOCUS       DAOSOL010000018         8387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_8432_length_8387_cov_4.842895, whole genome shotgun sequence.
ACCESSION   DAOSOL010000018 DAOSOL010000000
VERSION     DAOSOL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8432_length_8387_cov_4.842895"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PK171_00950"
     CDS             complement(<1..1378)
                     /locus_tag="PK171_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT04803.1"
                     /translation="MEENKNLEMLHEDIENIRPIRPGELIKGKVIKKGEEGYFVNINY
                     KAEGILPFKEKLTDGEGEILNDTEEGEEIWVVVNRFDDQGYVWLSREKARYRGAWIDI
                     EESMNSGKPLIAKVLKKVKGGLTVNIGVNAFLPASCVDKVPQNLDEYLDKTLTVKVIE
                     ADRRTRNVVVSHKVILEEEDLKKKQETIATLEIGQIRKGIVKNITSFGAFIDLGGIDG
                     LLHISEISWGRIGKLEDIFNKGDEIDIRVIGWDPVKEEISLSMKRLKPDPWENIEEKI
                     KVGDVVQGKVISIKDFGVFVETEEGVEGLVHISDISWGYVKHPQEAVKIGDMVDARIL
                     DIDKEKKRLSLGLKQIHPDPWLSVGENYPVDSVARVRVERINPKGAIVEIEDGVEGKI
                     PIEELSWKRVNRVSEILRRNQLIDARIVDVDPENRQITLSLKQMKENPWEQAQNLWKV
                     GDTVDGNVQ"
     gene            complement(1643..3409)
                     /gene="aspS"
                     /locus_tag="PK171_00955"
     CDS             complement(1643..3409)
                     /gene="aspS"
                     /locus_tag="PK171_00955"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT04804.1"
                     /translation="MLRTHNCGALRKEDIGEEVLLAGWVNRRRDHGGVIFIDLRDRWG
                     LTQLVINSNQPEIYQSANAVKSEFVLQVKGIVRARPEGLANLKLPTGEIEVAVSEVKI
                     LSESKPLPFEIEEKNETDESLRLHYRYLDLRRTRMQKNLILRHQVCQSVRRYLSERQF
                     IEVETPFLTKSTPEGARDFLVPSRLSEGNFYALPQSPQLFKQILMIAGYDRYFQIAKC
                     FRDEDLRADRQPEFSQVDIEMSFVEREDIFELIETMMKKLFYEVLGIQLETPFPRMSY
                     DEAMNCYGCDKPDLRISCTIDDLSLLLEDKIEVGSKSDSWNGLFLQEWKGFSRKKADL
                     LSQMAKNAGVSLSYIKFAKEEGTSPLKNKISEQAWSKIIEKYPFQEDSLLLMAWGERP
                     KVLTFLGNLRINLGEELNLIQNQFKFCWILDFPLFEWNAEENRWDSMHHPFTAPRLDQ
                     LDQLNIDPSQVKAQAYDIVLNGFEIGGGSIRIHRSDLQEKIFQLLNLNEGEIQQKFGY
                     FIEALQYGCPPHGGIALGLDRIMMLMSGESSIRETMAFPKTQKGTCLMSGAPSPVSND
                     QLMVLGIQLREKKESKAIKEDS"
     gene            complement(3393..4679)
                     /gene="hisS"
                     /locus_tag="PK171_00960"
     CDS             complement(3393..4679)
                     /gene="hisS"
                     /locus_tag="PK171_00960"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT04805.1"
                     /translation="MEKITLPKGTKDIGDPEIFIWYHVENIVRQEATHYHYIELRTPL
                     FEHTELFARGVGEETDIVQKEMYTFLDKGGRSVTLRPEGTAPVMRSYLEQSFHIHNPR
                     MKYFYMGPMFRYERPQAGRMRQFHQFGFEAIGFQSPAADAEIILLADRIYKKIGLKNI
                     RLEINSLGCRKCKPQYLETLKNYLRNQAAQFCKNCSNRIETNPLRVLDCKNESCQNIL
                     HNESFPIIQNFLCDECCTHQNHLIGILESAQIHPVINPYLVRGLDYYIKTTFEMKTEE
                     LGAQNAIGGGGRYDNLSDALGVKGIPGVGFAAGMERIILLLQKQSESKAFFQKPFIYL
                     SPLDQQGENVLLKLSIVLDEKSIPFYLDFSDKSLKYHFKNAQRMDASFIIIAGENEHQ
                     TKTYTLKNLLSGEQKVVSQKTLIGDLLEAYQNVKNT"
     gene            complement(4736..5398)
                     /locus_tag="PK171_00965"
     CDS             complement(4736..5398)
                     /locus_tag="PK171_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT04806.1"
                     /translation="MFNLTFDENIQIFIRLILAFLAGGLIGYQREKAEKPAGLRTHIL
                     VCIGSALITLVSIYGFGKMGSDPSRIVAQIVSGIGFLGAGTIFRFGTSVKGLTTAASL
                     WAICGVGIAIGVGLYFAALLSVLFIMAVLALLELFEQRLRTRGVHLIQVLISDIPGTI
                     GKIGEKLGEMEVNIKNIKMLREGKSLMRCYLWLDLPAHLSLTMVLSMLQELDTVQEVE
                     VD"
     gene            complement(5391..6347)
                     /gene="rbsK"
                     /locus_tag="PK171_00970"
     CDS             complement(5391..6347)
                     /gene="rbsK"
                     /locus_tag="PK171_00970"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOT04807.1"
                     /translation="MMSSRILIVGSLNIDMILKIDRMPETGENLFARQFTMLPGGKGN
                     NQAIACARLGLMVYMVGKVGDDDFGARLRLNLEQENVDFNFVTKQALTHTGLAFIFID
                     TKGENRILVAPGANMKLSVDDLENASQLFKTCDYLILQLEVPHSVVKSAIQKAHENNV
                     KVILNPAPAQPFPIEILTENDVITPNQYEAEVLSGVKIRSIEDAVQALKALDQRCPSR
                     KVITLGEKGALASDSGNRCIHLVPRKIDVQDTTAAGDAFNAGLAYGLSKGMDWITTLQ
                     LANNAGAIACTRIGAQSALPYLRDIDSFYSTQGTGEIRYFDV"
     gene            6966..>8387
                     /gene="purH"
                     /locus_tag="PK171_00975"
     CDS             6966..>8387
                     /gene="purH"
                     /locus_tag="PK171_00975"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT04808.1"
                     /translation="MIPIKTALVSVSDKTGLADLATYFSKHNISMIATGGTAKFIREL
                     GYPVKEVSEATGFPEILGGRVKTLHPIIEGGILARRDLEGDIIDLNTHNINPIDCVVC
                     NLYPFEKMIDSGETSLEKVVEEIDIGGITLLRAAAKNFKHVVILSSPSQYPSLIEELE
                     KNHGHFPSHLSGQYAIQAFLKSSEYDSHIANYLSQLMDNQTDEFPSCLSIIMKKRNSL
                     RYGENPHQKAALYEDLSLDRQGFMNAYQQFGGKELSFNNLYDTQAAYSLVREFNQPTT
                     VIVKHNNPCGVANDPILAQSAAKALECDPASAFGGIIAFNRTVDPETAMVFKTLFIEV
                     VIAPDFHPDALKIFEAKKNLRILKAPLSVPAEYDIKKLDGGYLIQNPDRLSYDPNQLK
                     TVTNLSPNDQERESLLFGWIVAKHVKSNAIILTKGLQTVGIGAGQMSRIDSLKIACIK
                     AEGKEVGSVLASDAFFPFPDVVEA"
BASE COUNT         2477 a         1870 c         1471 g         2569 t
ORIGIN      
        1 tttgaacatt cccatcaact gtatctccaa ccttccaaag gttttgggcc tgctcccaag
       61 gattttcttt catctgcttt aggcttaaag taatttgacg gttctcagga tcaacgtcga
      121 ctatacgggc atcaattaac tgattccggc gtaaaatttc gcttactcga ttcacacgtt
      181 tccaggatag ttcttcgatg ggtatctttc cttcaacacc atcttcaatt tcaacaatgg
      241 cacccttggg gttgatccgt tctacccgca ctcgagctac actatcaacc ggataatttt
      301 caccgactga aagccacggg tcgggatgga tttgttttaa accaagtgat aaacgttttt
      361 tttctttatc aatatcgaga attcgagcat caaccatatc ccctatttta actgcttctt
      421 gcggatgttt aacatatccc caggatatat cagaaatatg aaccagaccc tcaactccct
      481 cttcagtttc gacgaatact ccaaaatctt ttatggatat gactttgcct tggacgacat
      541 caccgacttt tattttttct tcaatgtttt cccatggatc aggtttaagg cgcttcatgc
      601 ttaaggatat ttcttcctta accgggtccc agcctatcac tcgaatatct atttcatcac
      661 ctttgttgaa tatatcttct aacttaccga ttcttcccca ggatatctct gaaatatgca
      721 acaatccatc gatgccacct aaatcaataa aagctccaaa actggtaata tttttaacaa
      781 tccctttcct gatttgacca atttcaaggg tagcaatggt ttcttgtttc ttttttaaat
      841 cctcttcttc aaggatgacc ttatgcgata ctactacatt ccgagttctt cgatcggcct
      901 caatcacttt tactgttaaa gttttatcaa gatattcatc taaattttga ggaaccttat
      961 caacacagga cgcaggaagg aaggcattca caccaatatt aacggttaac ccccctttta
     1021 cctttttcag tacttttgct attaggggtt tcccgctatt catgctctct tcaatgtcaa
     1081 tccaagcccc tcgatatcgg gctttttcac gagacaacca aacatagcct tgatcatcaa
     1141 accggttgac gactacccag atttcttctc cttcttcggt gtcgttaagg atttcccctt
     1201 caccatcagt gagcttttct ttaaaaggaa gaatcccttc tgccttataa ttaatattga
     1261 caaaataacc ctcttctccc tttttgataa cctttccctt aatcagctca ccgggtctga
     1321 ttggccggat gttttcgatg tcctcgtgca gcatctcaag gtttttgttt tcttccacag
     1381 tgaaacactc cttcctccgg gtttccttct atcacgaatt atagccccac attgtggtgt
     1441 gccatttgct ttgatccttg ccctcaaaaa tagtgggtgc cctctccagc aaaccacaat
     1501 ttgctggaag attgactccc agggaaagga tgttgggtca aagccaacta tggcatcacc
     1561 tgcatagcag ggctacaaac ccaaatcacc tttttgctaa ataataacat aacctgtata
     1621 gcatttcaaa ttgaattccg tattatgaat cttcctttat tgccttagac tctttctttt
     1681 ccctcaattg tattcctaat accataagct ggtcattgga aaccggcgat ggagcacccg
     1741 acataagaca ggtccccttc tgggttttgg gaaacgccat agtttcacga atggaacttt
     1801 caccactcat gagcatcatt atacgatcca atcccaaagc tatgcctcca tgagggggac
     1861 aaccatattg gagggcttca ataaaataac cgaatttctg ctgaatttct ccctcattta
     1921 agttgagcaa ttgaaatatt ttctcctgca aatcggaccg gtggattctg atacttcctc
     1981 cacctatttc aaaaccattg agaacaatat cataggcttg agcttttacc tggctgggat
     2041 cgatatttaa ttgatctaac tggtccaaac gaggagctgt aaaaggatga tgcatcgaat
     2101 cccatcggtt ttcttcagca ttccattcaa aaagcggaaa atcaaggatc caacaaaact
     2161 taaattgatt ttgtatgagg ttgagttctt cacccaaatt tattcgcagg ttgcccaaaa
     2221 aagtaagaac ctttggtcgt tcaccccagg ccatgagcaa aagcgagtct tcttgaaatg
     2281 gatatttctc aataattttg ctccaggctt gctcagaaat tttatttttt aaaggtgagg
     2341 tcccttcttc tttagcgaac tttatatagg agagagaaac tccggcgttt ttagccattt
     2401 gagagagaag gtcagctttt tttctagaga aacctttcca ttcctgaaga aacaatccat
     2461 tccaggaatc actttttgat ccaacttcaa ttttatcttc caacagcaac gaaagatcat
     2521 caatggtaca ggaaatccta aggtctggtt tatcgcaacc ataacaattc atcgcttcat
     2581 catagctcat tcggggaaag ggggtttcta attgtattcc taacacttca taaaatagct
     2641 ttttcatcat ggtttcaatt aactcaaaaa tatcttcccg ctcaacgaag gacatttcta
     2701 tgtccacctg agaaaactct ggctgccgat cagcacgaag gtcttcatcg cggaagcatt
     2761 tagcaatttg aaaataccga tcataaccag ctatcattaa aatttgttta aaaagttgcg
     2821 gtgattgggg aagcgcataa aagtttcctt cactcaaacg actgggtacc aaaaaatcac
     2881 gagctccctc aggagtgctc ttggttaaga atggggtctc gacttcaata aactgccgtt
     2941 cagaaagata gcggcggact gattgacaaa cctgatggcg aaggatcaga tttttttgca
     3001 ttcttgtcct acgaagatct aaatatcgat agtgcagcct taaactttca tcggtttcat
     3061 ttttctcttc aatttcaaaa ggaagaggtt tcgattcgga aagaatttta acttcagata
     3121 cggctacttc aatttctccg gtgggtaact tcagatttgc caaaccttca ggccgagctc
     3181 gaactattcc ttttacttgg agaacaaact cactttttac agcattggca ctttggtaaa
     3241 tctctggttg attggaatta ataaccagtt gagtcaaacc ccaccggtcc cgaaggtcta
     3301 taaagataac cccaccgtgg tcccgacggc ggttaaccca tccagccaga agaacttctt
     3361 ccccaatatc ttccttacgt aatgccccac aattatgtgt tcttaacatt ttggtatgcc
     3421 tccagcaaat caccaattag agtcttttga gaaactactt tttgttcccc agatagcaaa
     3481 ttttttaagg tataagtctt cgtctggtgc tcattctctc cagcaataat aataaaagaa
     3541 gcatccattc gctgagcatt tttaaaatgg tatttaagag atttatctga aaaatctaaa
     3601 taaaagggaa ttgatttctc gtcaagaacg atcgaaagct ttaataaaac attttctccc
     3661 tgttgatcaa gaggagataa ataaataaat ggcttctgga aaaatgcttt tgactcgctc
     3721 tgtttttgca aaaggagaat aattcgttcc atcccagcag caaaaccgac cccagggatg
     3781 ccttttactc ctaaagcatc ggataagtta tcgtatcggc ctccgcctcc aatagcattt
     3841 tgggctccca attcttcggt cttcatttca aaggtggttt ttatataata atcaagacct
     3901 cttaccaaat aaggattaat aaccgggtgg atttgagcgc tctcgagaat tccgatcaag
     3961 tggttttgat gagtgcaaca ctcatcacag agaaaatttt gtataatcgg gaaactttca
     4021 ttatgaagta tattctgaca agattcattt ttacaatcca gcactcggag gggattggtc
     4081 tcaatccgat tggaacaatt tttgcagaat tgagctgctt gatttcgcaa ataatttttt
     4141 aaagtttcga gatattgcgg cttacatttt ctacatccca aactattgat ttctaatcgg
     4201 atatttttta atccaatttt tttatatatc cgatcggcca aaagaataat ctccgcatcg
     4261 gctgctggac tttgaaatcc gatcgcctca aagccaaatt ggtggaactg ccgcattcga
     4321 ccggcttggg gtcgttcata acggaacata ggtcccatat aaaaatattt catccgagga
     4381 ttgtgaatat ggaagctctg ttcgagatag gacctcatta ccggtgccgt tccttccggt
     4441 cggagggtta cactgcggcc tcctttatca agaaaggtgt acatctcttt ttgaacaatg
     4501 tcggtttcct ctccaactcc tcgagcaaaa agctcagtat gttcaaaaag aggtgttctt
     4561 aactcgatat aatggtaatg ggtggcctct tgcctgacaa tattttcgac atgataccaa
     4621 ataaaaattt cgggatctcc gatatctttt gttcctttag gtaaagtaat tttttccatt
     4681 ttttctttaa ttttccttct tgttcctcag tttatcttta tttcaataaa aaactttaat
     4741 caacttcaac ttcttgaacg gtatctaatt cctgaagcat gcttaaaacc atcgttaagg
     4801 aaagatgggc ggggaggtct aaccagaggt agcatcgcat caaacttttt ccttctcgaa
     4861 gcatttttat attttttata ttcacttcca tttctcctaa tttttcccca atttttccaa
     4921 ttgtccccgg aatatctgat atcagaactt ggatcaaatg gactcccctg gtccttaatc
     4981 gctgttcaaa gagttctaat aaagccaata ctgccataat aaataaaact gataatagag
     5041 cagcaaaata aaggccaact ccaatagcaa taccaactcc acagatcgcc cataagctgg
     5101 cagcagttgt taaccctttt accgaagttc caaaacggaa aatcgtccct gctccaagaa
     5161 atccaattcc actgacaatt tgtgccacga tgcgggaagg atctgaaccc attttcccaa
     5221 agccataaat cgatactaaa gttattaacg ctgaaccgat acaaaccaat atatgagtac
     5281 gtaaaccggc tggtttttca gctttttcac gctgataacc aataaggcca ccagccaaaa
     5341 aagccagaat caatcgtata aaaatttgaa tattttcatc aaaggttaaa ttaaacatcg
     5401 aagtatcgaa tttcaccagt gccctgagtc gaatagaaac tatctatatc ccgtaaataa
     5461 gggagagcgc tctgagcacc aattcgggta caggcaatag caccggcatt atttgccagt
     5521 tgaagagtcg ttatccaatc cattcctttg gataatccat aagccaaacc agcattaaaa
     5581 gcatcaccgg cggcggtggt atcctgaacg tcaatttttc tgggaaccag atggatgcat
     5641 cggtttccag aatcactggc caatgctcct ttttctccta aagtaattac cttacgagac
     5701 ggacatcgtt gatccaaagc cttcaaggct tggacggcat cttctatgct tcgtatcttt
     5761 actccgctca atacttcagc ttcatattga ttgggggtta taacatcatt ttcggtcagg
     5821 atttcaatcg gaaaaggctg agccggtgct ggattgagaa taacttttac attgttttcg
     5881 tgagcctttt gaatcgcact ctttaccacg ctatggggaa cttccagttg aagtatgaga
     5941 taatcacaag ttttaaaaag ttgggaggca ttttctaaat catcaaccga aagcttcatg
     6001 tttgctccgg gcgcaactaa aattcggttt tctcccttgg tatcgataaa aataaaagcc
     6061 aaccctgtat gagttaaagc ttgtttggtg acaaaattaa aatcaacatt ttcctgctcc
     6121 aaatttaagc gaaggcgtgc tccaaaatca tcatctccga ctttaccaac catatacacc
     6181 atgagcccca agcgtgcaca agcgatagcc tggttatttc cttttcctcc gggtagcatg
     6241 gtaaattgac gggcaaagag attttcccca gtctcaggca ttcgatcaat tttcagaatc
     6301 atatcgatat ttaagctacc aacaattaaa atacgagaac tcatcatttt tccctcacac
     6361 ctcattccaa aaccggttgt aaaatatata taatattact caagataacc ctgaacgtcc
     6421 agtttttcaa agaaattcat ggttttaatt tgaattaagg attaccatgg aaagtcatca
     6481 taaaaaaata cctatccaat tcttcatggc aaaaagtccc aaccttcttt agttgaacga
     6541 cgtggcaatc tcatttagca attctttcat tctgaagaat ccatggtttt ttacctgaca
     6601 ttataagaat tccattcatt ccaattccac aattccataa tttctcaatc ctacaaataa
     6661 taaaagataa ctcatgagat tgccacgacc tcaaatagcg aggtctcgca atgacgacta
     6721 aaagttcctt ctcacttgat gggagaaggt gaggatgagg gtgaatactt ttccattcac
     6781 taatgacaac tcacctctca ccgcatttac aatataaatg gaattgaaaa ttaaactcct
     6841 tttaagaata atttctcttt ttaaatgaaa acattataat aaccaataaa ctgattaaaa
     6901 tgatgaattg aaaaacccaa ataaacggtt ttttcgatat actttctaaa aatggaggta
     6961 acaacatgat accgattaag acggcgttag taagtgtttc tgacaagacc ggtttggcag
     7021 atttagccac ttacttttcg aaacacaata tttccatgat tgctaccggt ggaactgcaa
     7081 aattcattcg tgaattaggt tatccagtca aagaggtttc ggaagcaacc ggtttcccag
     7141 aaatcttggg gggtcgggta aaaactctcc atcctattat cgaaggtgga attttagcac
     7201 gccgcgatct ggagggagac ataattgacc tcaataccca caatattaac ccgattgatt
     7261 gtgtcgtttg caacctatat ccttttgaaa aaatgataga ttcaggagaa acttctttgg
     7321 aaaaggtagt tgaggaaatc gatattggcg gaattaccct cctccgggct gcggcaaaaa
     7381 atttcaaaca tgttgttatt ttatcctctc cttcgcaata tccttcattg atagaagaat
     7441 tggaaaaaaa ccatgggcac ttcccttccc acttgtctgg acaatatgcc attcaagctt
     7501 tcttaaaaag ctcggaatat gactctcata tcgccaatta cctatctcaa ctaatggata
     7561 atcaaaccga tgaatttcct tcctgcttat cgattataat gaagaaaaga aactcccttc
     7621 gttatggaga gaatcctcat caaaaagccg cattgtatga agacctttct cttgatcgtc
     7681 aaggcttcat gaatgcctat caacaatttg gcggaaaaga attgtcgttt aataatctct
     7741 atgataccca ggcagcctat tctttggttc gggaattcaa ccaaccgact acggttattg
     7801 tgaaacataa taatccctgt ggagtagcaa acgatccgat actcgctcag tcggctgcga
     7861 aagccttgga gtgtgaccca gcatcagctt ttggtggaat cattgccttt aaccgaaccg
     7921 tcgatcccga gacggcaatg gtttttaaga ccttatttat cgaggtggtt attgctccag
     7981 actttcatcc tgatgcacta aaaatttttg aagcaaagaa aaacttgaga attttaaaag
     8041 ctcctttaag cgttccagct gaatatgata tcaaaaaatt ggatggcgga tatctcattc
     8101 agaatcctga ccggcttagc tatgatccta atcaattaaa aaccgtaacc aatctttccc
     8161 ccaacgatca ggaacgagaa agccttttgt ttggatggat agtagccaaa catgtcaagt
     8221 ccaatgctat cattcttact aaaggattac aaaccgtagg aatcggtgct ggccaaatga
     8281 gtcggattga ttctctaaaa attgcctgca taaaagcgga aggaaaagaa gttggatcgg
     8341 ttttggcctc tgatgccttt ttccccttcc cagacgtagt cgaagct
//
LOCUS       DAOSOL010000019         8362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_8456_length_8362_cov_3.964608, whole genome shotgun sequence.
ACCESSION   DAOSOL010000019 DAOSOL010000000
VERSION     DAOSOL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8456_length_8362_cov_3.964608"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /gene="cbiD"
                     /locus_tag="PK171_00980"
     CDS             complement(<1..565)
                     /gene="cbiD"
                     /locus_tag="PK171_00980"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HOT04809.1"
                     /translation="MKLGITTGTCASAAALGALFKALGKDPSYVKTQLPNGEYIDVEI
                     IQAGVRSNGHYFSMVKKYAGDDPDVTDGILIGASLLLRPGKQNISIIGGEGVGKVTKP
                     GLYLEPGEWAINPVPKKQIIDNLLRFLPKYWDLQVEIFVPYGSKIATQTFNSELGIEG
                     GISILGTTGRVFPMSQGSFMETIRLQIR"
     gene            complement(562..1251)
                     /locus_tag="PK171_00985"
     CDS             complement(562..1251)
                     /locus_tag="PK171_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOT04810.1"
                     /translation="MKYFFDIDPGHQAFLILKEVDSMDFIESQSLQLIKTVLDHYSLP
                     DYGRFLVERAVHATADFSIASLFVIQPGFIETSTNKIETGSEIYCDTEMVSSGISPHL
                     LQQCGIKLTVFVHQQECYARAQRDNITRSEAAVDLSCEKNIRKFIFGNAPTGLLRLVQ
                     HINQGYRADFVVGMPVGFISAAISKKQLLQTGVPAVVLSGPRGGSSIAASVFNALLKK
                     KLEENGMEIIR"
     regulatory      complement(1275..1444)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1538..2920
                     /locus_tag="PK171_00990"
     CDS             1538..2920
                     /locus_tag="PK171_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOT04811.1"
                     /translation="MTRGIVIAAPYSGSGKTTVTLGLIRALTRQFRVIPFKIGPDYID
                     PSYHQAAAKNEPHNLDLFLLGEENLKKIFFKSIKKGEMAIVEGVMGLFDGLGTESWAS
                     TAHVAKILKLPVILVVDVRSMSTSAAALVHGFQTYDPELQLVGVICNLTSSKTHYNHV
                     KKSIERDCNLPVVGHLPFKKFLDMPERQLGLIPAWHIIDIDKWLDHCADFLLKNVNLN
                     QIIDLMKPLEKVDVSSTFQVKLPPSETIRIGIAYDGAFHFYYSDGLETLRSAGAELVS
                     FSPMNDKFLPEGLDGLILGGGFPETNALGLSYNQSMRNSIRSAIENGLPTYAECGGMM
                     YLTQEIEDYTHRFYPMVGIFPVKAIMGKRLQHFGYVEVKVTRNNVLAPTDTILHGHEF
                     HYSELIGSLPISSYKVKKPNQKNTWDSGFVYKNCLASYVHLHLSAYPELVSNFLNHCR
                     LWKKNHSYLI"
     gene            3387..5051
                     /gene="argS"
                     /locus_tag="PK171_00995"
     CDS             3387..5051
                     /gene="argS"
                     /locus_tag="PK171_00995"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOT04812.1"
                     /translation="MSRLITDVIKESLQKVVKEQYGNLFEELLFTVEPTRDKAHGDFA
                     SNIAFLLSKKARKSPREIANQIVSILNQSIGHIATIEIAGGGFINFFLKDHIIYSFMQ
                     DIYQEKDEYGSVHVGNNEKMQVEFVSVNPTGPLHVGHGKCAAFGDALARVLKKAGFIV
                     EKEYYINDAGRQIDLLGLTLECRYKQLFGEEIEIPEGGYKGDYMIDIAQDLKKEKGDT
                     LLSLTKDEKNEIFNSYAVSKILQSIQKDLSDFQVNFDVWFSEKTLYQNHEVEKALNQL
                     VKQGFTYEKDGALWLRTQQWGDDKDRVLVRENGSPTYFASDIAYHFNKWQRGFERVID
                     IWGADHHGYIPRMKAAVNALGLKKEFLEIFIVQFVTLLRNGQPERMSTRQGEFIPLRN
                     LINEVGVDVARYYFLMRDPATHLEFDIEEAKKTSMDNPVYYVQYAYARISSVFREMEK
                     KDLSNIKIDQKHESFHNEQEKLLAKNLIYFPEVVKRSALARQPYMICNYVTDLAGSFH
                     TFYNNNRILDEENLELTAFRLNLCRATRQVLKNALDLLGISAPETM"
     gene            5067..6710
                     /locus_tag="PK171_01000"
     CDS             5067..6710
                     /locus_tag="PK171_01000"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT04813.1"
                     /translation="MNGKEGKFIFITGGVTSSLGKGITASSIGRILKSRQLKITMQKF
                     DPYINVDAGTMNPYQHGEVFVTQDGGETDLDLGHYERFIDEELSKSSNVTTGKIYSSV
                     IARERHGDYLGATVQVIPHITNQIKEEIFRLREECQADVSIVEIGGTVGDIESLPFLE
                     AIRQIKNDIGKSNCLYIHVTLVPFLDAAGELKSKPTQHSVKELRSIGIQPDMIICRSS
                     HVMTREVISKIALFCDIEKEAIIPVLDAESIYDVPITLEEKGVGNLIINKLQLNAAEV
                     DLHDWSEIISRMKNPNQKINIGIIGKYIESKDAYLSINESLKHGGGALGVKVCIKPIE
                     AEKIQEQNVEKYLGELDGVLIPGGFGKRGIEGKIIAARYARENNIPFFGICLGMHTAV
                     IEFARNAAGMVGAHSTEFNPETPFPIIHLLPDQRTKKDLGGTMRLGNYRCQLKTNTKS
                     YSLYQQEEIMERHRHRYELNSEYIHQLEKSGMIMVGFNPEFQVVEMVELFNHPWFVGV
                     QFHPEFKSRPNRPHPLFYGFVQAAQKYHQNQRIGEKTFG"
     gene            6703..7569
                     /gene="murI"
                     /locus_tag="PK171_01005"
     CDS             6703..7569
                     /gene="murI"
                     /locus_tag="PK171_01005"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT04814.1"
                     /translation="MGSNSNPIGVIDSGVGGLSMVIALDQLLSSEEIVYVADPLHFPY
                     GEKAKNELIHIVSSLVSYLNENLKAKLIITACGTISSNCLEELSALFQVPIIGIIDPA
                     SREAVRKTKTGKVVVLATSATVKSSTFQKHLQRIHSSITVKEEAWPEFVEAVEKGLHQ
                     TDDWRKWIHTQLIGFQQENFDTIIMGCTHFALISSFFQDIAETTFEIINPAIATAQEV
                     KEYLAKSNLLNPSELSKKRIIVRGNSTNIKKTIQKYSDLQKINIEAFPEKIIENFINK
                     VQRYCTPASTPM"
     gene            7591..>8362
                     /gene="ispE"
                     /locus_tag="PK171_01010"
     CDS             7591..>8362
                     /gene="ispE"
                     /locus_tag="PK171_01010"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT04815.1"
                     /translation="MKNDTIYRFRSYAKINWFLKIGQQRTDGYHDILSVMQLINFYDI
                     IDFYFNNQNCDEIQCNYEIPTGKKSLLGRLFHLLRELNPHIKERYFGVKIIKNIPPSS
                     GLGGASSNVANLLKKMNEILQLSFENEKLIELSAKLGSDIPFFTTGYPFAIISGKGEK
                     VIPLHQPPEKYIVLVFPKVGIDTSWAYREWDIEQEQQNCQNLPSVKNFLIDFNPSQIE
                     KYIWNDFEKIVYKRMPEFAEYKKTLEQMGCSSVFMTGSG"
BASE COUNT         2690 a         1511 c         1668 g         2493 t
ORIGIN      
        1 gacggatttg taaacgaatc gtttccataa aagaaccttg cgacatgggg aagactcttc
       61 ccgtagttcc taagatagaa ataccgcctt ctataccaag ctcggaattg aaagtttggg
      121 ttgctatctt cgatccatac ggaacgaata tttcaacctg aaggtcccaa tacttaggaa
      181 gaaatcgcaa taagttatcg atgatttgtt ttttgggaac tggattaata gcccattctc
      241 ctggttcgag atataaacca ggtttggtta cttttccaac tccttctccc ccaataatcg
      301 agatattctg ttttcctggt cttagtagga gagaagcacc gatcaatata ccatcggtga
      361 catccgggtc atcgcccgca tactttttca ccattgagaa atagtgtcca ttggatctta
      421 cgccagcttg aataatttca acgtcgatat attctccatt gggaagctga gtcttgacat
      481 aagaagggtc ttttcctaag gctttaaaaa gtgctccaag agcggcagcc gatgcacaag
      541 ttccagtagt gattcccaat ttcatcttat aatctccatt ccattttctt ctaacttttt
      601 tttgagaagg gcattgaaaa ctgaagcagc tatagaactt cctccccggg gaccggagag
      661 aacgacagct ggaacaccgg tttgtaacaa ttgtttttta gaaatagctg ccgaaataaa
      721 accaaccggc atgccaacga caaagtcagc ccgatatccc tggttgatat gttgaactaa
      781 ccgaaggaga ccagttggag catttccaaa aataaattta cgaatgtttt tttcacaact
      841 caaatctaca gctgcttctg agcgggttat attatcacgt tgagctcgtg cataacattc
      901 ttgttgatga acgaaaacag taagtttgat tccacattgc tgaaggagat gaggagatat
      961 tccactggaa accatttcgg tatcacagta aatttcggaa ccggtttcga ttttattcgt
     1021 tgaggtctca atgaaacctg gttgaataac aaaaagtgaa gcgatcgaaa agtcagcagt
     1081 tgcgtgaacg gctcgctcga cgaggaaacg accataatca ggaagggaat aatgatctaa
     1141 aacggtctta atcagttgaa gactctgtga ttctataaaa tccatgctat cgacctcttt
     1201 caatattaaa aaagcctgat gtcccgggtc gatatcaaaa aaatatttca actttcgtct
     1261 cgcgcaaaag ctgaaaactg atatattccc cgggccggtc tcctggcttg aaggcttttc
     1321 ctactcggtt ttccttcccg gcgacatcgc cagtggcata acaaaccttt cgttccttct
     1381 cacagtggcg ggaccacacc ggattttcac cggtttcccg aacacccgtt gaatgtttct
     1441 attcagttgt ttttatcttc atcacatggt agattaaatc attattttca atttgacaag
     1501 aggtttctta taaaatttat taaaggatgt tacaaagatg acgcgaggaa ttgtcattgc
     1561 agctccctac agtggttcgg ggaagacaac agtaactctt ggcttgatac gggcattgac
     1621 ccgccaattt cgggttattc cttttaagat tgggccggat tacatcgatc cttcctatca
     1681 ccaagctgct gctaaaaatg aaccacacaa cctggatcta tttttactgg gagaagagaa
     1741 cttaaaaaaa atatttttca agtcgattaa aaaaggagaa atggccatag ttgaaggagt
     1801 aatgggttta ttcgatgggt taggaaccga gtcttgggca agcaccgctc acgtggccaa
     1861 aattcttaaa ctaccagtaa tcttggtagt cgatgtacga tcgatgtcaa ccagtgcggc
     1921 agctctggtt cacggctttc agacctacga tcccgagctt caactggtgg gagttatctg
     1981 caatctaacc tcaagtaaaa ctcattataa tcatgttaag aaatccattg agagagattg
     2041 taacctccca gttgttggtc atcttccttt taaaaaattt cttgatatgc cagaacgcca
     2101 gctgggtctg atccctgctt ggcatataat tgatatcgat aaatggcttg atcattgtgc
     2161 cgattttctt cttaaaaacg tgaatctcaa ccagatcatt gacctaatga aacccttaga
     2221 aaaagtcgat gtcagctcaa cttttcaggt aaaattacct ccatcagaaa caattcgcat
     2281 cggaattgcc tatgacggag cattccattt ttactactct gatggtttag aaactcttcg
     2341 atctgccggt gctgaattgg tttcctttag cccaatgaat gacaaatttc ttcctgaagg
     2401 attggatggg cttattttag ggggaggctt cccagaaaca aacgctttag ggctctctta
     2461 taaccagtcg atgcggaatt ccattcgatc ggcgatcgaa aatggtcttc cgacctacgc
     2521 tgaatgtgga ggaatgatgt atttaactca ggaaattgaa gattataccc atcgttttta
     2581 tccaatggtg gggatttttc ccgttaaggc gattatgggt aaacggctcc agcactttgg
     2641 gtatgttgaa gtcaaagtaa cccgaaataa cgtcctcgca ccgactgata cgattcttca
     2701 tggtcatgag tttcattatt ctgaactaat cggttcgtta ccaatatcca gctataaggt
     2761 aaaaaaaccc aatcagaaaa atacctggga ttcaggcttt gtttataaaa attgtttagc
     2821 ttcctatgtg cacctccatt tatcggctta tccagaatta gtttctaatt ttctcaacca
     2881 ttgccgtcta tggaagaaaa accactctta tctcatttag taaatttttt taaaataatt
     2941 aaaggatgag atcctcacgc cctcactttg tgagggctta ggatgaccga ttaaagaatt
     3001 cattagatgg tattttctgg atagaccttc atgccacttg tgtagcacca gatgaagatg
     3061 aatataatta tcccactcac cctaacactt tcccactcgg ggagacaaaa aaaagagagc
     3121 aatggaaaaa gtagaaaata agagttttag attttattct tgaaggaata gaaaaaaaaa
     3181 tatataatgg cgcaggagat atcatacttt gattttcaac ttgcatagtt catctgcgtt
     3241 tattagcatt tgaaaaatgt tttttaaaaa atccattata aaccggctat tcattaaaat
     3301 tgaatttaaa aaagttattt aatattgata aatggaccaa tgagggtcca ttttttgtgt
     3361 tttaaacttg gtgtggagtg agtcagatga gtcgtttaat aaccgatgtt atcaaagaaa
     3421 gtcttcaaaa agtagtcaaa gaacaatatg gaaacttatt cgaagaatta ctttttacag
     3481 ttgagccaac tcgtgataaa gctcacggag attttgccag caacatagcc tttttgcttt
     3541 caaaaaaagc caggaaatcc cctcgagaaa ttgccaacca aattgtatcg attcttaacc
     3601 aatctatcgg tcacattgca actattgaga ttgccggcgg aggtttcata aactttttcc
     3661 tgaaggatca tattatttat tcatttatgc aggatatata tcaggaaaaa gatgagtatg
     3721 gatcggttca tgttggcaac aatgaaaaaa tgcaagtaga atttgtcagt gttaatccaa
     3781 ccgggccact ccatgtaggc cacgggaaat gtgcagcctt tggtgatgct ttggcaagag
     3841 ttttaaaaaa agctggattt atagttgaaa aggaatatta tataaatgat gccggtcggc
     3901 agatagattt attaggtctt accttggaat gccgttataa acagcttttt ggtgaagaga
     3961 ttgaaatacc agagggagga tataagggtg attatatgat tgacatcgcc caggatttga
     4021 aaaaagaaaa aggcgatacc cttttatctc taacgaagga tgaaaaaaac gaaatattta
     4081 attcttatgc agtctcaaaa atattgcaaa gcatacaaaa agatttgagt gactttcagg
     4141 ttaacttcga tgtttggttt agtgaaaaaa ctctctacca gaaccacgaa gttgaaaaag
     4201 ccttgaatca attagttaag caaggtttta cctatgaaaa ggatggagcc ctttggttaa
     4261 gaacccagca atggggagat gataaagatc gggtgctggt tagagaaaat ggatcaccta
     4321 cctattttgc ctcggatatt gcctaccatt ttaataaatg gcaaaggggt tttgaaagag
     4381 taattgatat ctggggagct gatcatcatg gatatattcc ccggatgaaa gctgcagtta
     4441 atgcattggg cttaaagaaa gaatttttgg aaatttttat tgttcaattt gtaactctgt
     4501 taagaaatgg acaaccggaa agaatgtcaa cccgacaggg tgaatttatc ccccttcgta
     4561 atttgattaa tgaggttggt gttgatgtag ctcgttacta ttttctcatg agagatcctg
     4621 caacccacct tgagtttgat attgaagaag ccaaaaagac ttcgatggat aacccggtat
     4681 attatgttca atatgcttat gctcgaatat catcggtttt tcgagaaatg gaaaagaaag
     4741 atttatccaa tataaaaata gaccaaaaac atgaatcatt tcataatgag caggaaaaat
     4801 tattagctaa aaatttgata tactttccag aagtggtaaa acgatcagct ttagcacggc
     4861 aaccctatat gatctgcaac tatgtgacgg atttagcagg ttcatttcat actttttata
     4921 ataacaaccg aattttagac gaagaaaatt tagagttaac ggcttttcga cttaatttat
     4981 gccgtgcaac tcgacaggtg ctcaaaaatg cactggattt attgggtatt tcggcaccag
     5041 aaacgatgta gggaaggtcg attagtatga atggtaagga aggaaaattt atttttatta
     5101 ccggaggagt cacttcctca ttaggaaagg ggattacagc atcatcaata ggtcgaattt
     5161 taaaaagtcg acaacttaaa ataaccatgc agaagttcga tccgtatatt aatgttgatg
     5221 ccgggactat gaatccctat caacacggtg aagtttttgt tacacaggat ggaggagaaa
     5281 ccgaccttga tcttgggcat tatgaacgct tcatcgatga agagttatcg aaatcaagta
     5341 atgtaacaac cggaaaaata tatagttcgg taattgctcg cgaacgtcat ggggattact
     5401 taggagctac cgttcaagtt attcctcata ttaccaacca gataaaagaa gaaatatttc
     5461 gtctcaggga agaatgtcaa gctgatgttt caattgttga gattggcggt acagttggtg
     5521 atattgaatc actcccattt ttggaagcta ttcgccaaat caagaatgat attggaaaat
     5581 cgaattgtct atatattcat gtcactctcg ttccctttct cgatgctgcc ggagagctta
     5641 aatccaagcc cacacaacac agcgttaaag aattacgaag cattggaatc caacccgaca
     5701 tgataatttg tcgttcttct catgttatga cccgagaagt tatatctaaa attgcacttt
     5761 tttgtgatat tgaaaaggaa gccatcattc ccgttcttga tgccgaatca atttatgatg
     5821 tcccgattac tttggaggaa aaaggagttg gcaatctcat cattaataag cttcaactaa
     5881 atgctgcaga agttgatctc catgattggt cagaaattat atctcgaatg aaaaacccca
     5941 accagaaaat caatattggg attatcggga agtacataga atcgaaggat gcctatttaa
     6001 gtatcaacga gtcgctaaaa catggtggag gagccttggg agtaaaggtt tgcattaaac
     6061 caattgaagc tgaaaaaatt caagaacaga atgtagagaa gtaccttgga gagctcgatg
     6121 gtgtattaat tccgggaggt tttggaaaaa gaggaataga aggaaaaatt attgctgcac
     6181 ggtacgcacg ggaaaataac attccctttt ttggaatctg tttaggaatg catacggcag
     6241 tgatcgaatt tgctcgtaat gctgctggaa tggttggtgc tcattcaact gagtttaatc
     6301 ccgaaacacc ttttccgatt attcatcttt tacccgatca gcggactaaa aaagatttag
     6361 gtggtaccat gaggttggga aattaccgat gccagctgaa aaccaataca aaaagctact
     6421 cactatatca acaggaagag attatggagc gacatcgcca tcgttatgaa ttaaactctg
     6481 aatatataca ccaattagaa aaatcaggaa tgattatggt agggtttaat ccagagtttc
     6541 aagttgttga aatggttgag ctcttcaatc atccctggtt tgtcggagtc caatttcatc
     6601 ctgaatttaa atcccgtcct aatcgacctc accctctttt ttatggcttt gttcaagcgg
     6661 cacaaaagta tcatcaaaac caaagaattg gagagaagac ttttgggtag taattcaaat
     6721 cctattggtg tgattgattc cggtgtcgga ggactcagca tggtcattgc tttagatcag
     6781 ttgctttcat cggaagaaat agtttacgtc gctgaccctc ttcattttcc ttatggagaa
     6841 aaggctaaaa atgaactgat tcatattgtt tcttcattgg tttcctactt gaatgagaat
     6901 ttaaaagcaa agcttataat aaccgcttgc ggaacaatca gctcaaactg cctggaggaa
     6961 ctgtcagctt tgtttcaagt tccaattatt ggtattatag atcctgcttc cagagaagct
     7021 gtaaggaaaa ccaaaaccgg caaggtcgtt gtgttagcga cttcagcaac tgtaaaaagt
     7081 agtactttcc aaaagcattt acaaagaatt cattcttcca ttaccgttaa agaggaagct
     7141 tggccggaat ttgttgaagc ggttgaaaaa ggactgcatc aaaccgatga ttggagaaaa
     7201 tggattcata ctcagctgat tggttttcaa caagaaaatt ttgataccat cattatgggt
     7261 tgtacccatt ttgctttaat atcatctttt tttcaagata ttgctgaaac tacgtttgaa
     7321 attattaatc cagcaatagc aactgctcaa gaagtaaaag aatatttagc aaaatcaaat
     7381 ttgttaaatc catcggagct aagtaaaaag agaattattg tgcggggaaa cagcacaaac
     7441 ataaaaaaaa cgattcaaaa atactccgat ttacaaaaaa ttaatattga ggcatttcct
     7501 gagaagatta ttgaaaactt catcaacaaa gttcagagat attgtactcc ggcctcaact
     7561 ccaatgtaaa aatcctggaa agatccccaa atgaaaaacg acactattta tcgattccgt
     7621 tcttatgcaa aaataaattg gtttttaaaa attggtcaac aacgtacgga tggatatcat
     7681 gatatattat cggtaatgca actgatcaat ttttatgata ttattgattt ttattttaac
     7741 aaccaaaatt gtgatgaaat tcaatgtaat tatgaaatac cgactgggaa gaaaagtttg
     7801 cttgggcgtt tatttcatct cctgcgtgaa ttaaatcccc acattaaaga gcggtatttc
     7861 ggtgttaaaa taattaagaa tattcctcct tctagtggtt tgggaggtgc tagcagcaac
     7921 gtagcaaact tattaaaaaa aatgaatgaa atactacaat tatctttcga aaatgagaaa
     7981 ctaattgaat tatcagcaaa actcggttct gatattcctt tctttacaac tggttacccc
     8041 tttgcaataa taagtgggaa aggcgaaaaa gttattcctc ttcatcagcc accggaaaaa
     8101 tatattgttc tggtttttcc aaaagtgggt attgatacca gttgggcata ccgagaatgg
     8161 gatattgaac aagagcaaca aaattgccag aatttgcctt cggtgaagaa ttttttaatt
     8221 gactttaatc caagccagat agaaaaatat atctggaatg actttgaaaa aattgtttat
     8281 aaacgcatgc cagaatttgc tgaatataaa aaaactttag aacaaatggg atgttcttcg
     8341 gtttttatga ccggaagcgg tt
//
LOCUS       DAOSOL010000020         8332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_8505_length_8332_cov_3.499456, whole genome shotgun sequence.
ACCESSION   DAOSOL010000020 DAOSOL010000000
VERSION     DAOSOL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8505_length_8332_cov_3.499456"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1047
                     /locus_tag="PK171_01015"
     CDS             70..1047
                     /locus_tag="PK171_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT04816.1"
                     /translation="MMRTRRLGKTNLELTVIGFGSWAIGGGGWRYAWGPQDDSESIEA
                     IKKALDLGINWIDTAAIYGLGHSEEIVGKAIKGFSKKPIIATKCGLVWDDKKNISNRL
                     KRESIKAEVEASLRRLDVDTIDLYQIHWPIPDEDIEEGWEAMAQLVKEGKVRYIGVSN
                     FNVSQMKRAQSIYPIASLQPPYSMLKRDIEEDILSFCNQNSIGVVVYSPMQKGLLTGK
                     MTRERIASFPADDHRRNDPEFNEPLISYNLEFVNQLIPIAKKHNKTLAQLAIAWALRR
                     PEVTSAIAGARHPDQIAETVLAGDWTLPQEDIDNIEKLFRERKQKIMNK"
     gene            complement(1533..2573)
                     /locus_tag="PK171_01020"
     CDS             complement(1533..2573)
                     /locus_tag="PK171_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT04817.1"
                     /translation="MTKKERVNHAIHHQKSDRLPHQIDFTWRMKKEFLNHFQIQEKEL
                     PELLGNHFLYIDTHKKVKLDEEKGVDYDVFGVGWDLVKSEGYLPCYHPLTDWKNFNSF
                     QFPDPQNSILYQDFIPLQDWQKKEYFILSTQGWVLMERAWLLRGFENFMIDLIDNRTE
                     LERLLDLITDYQIEVLRQLIQLGVDGIYTGDDYGTQRGLLIARKTWQDLLKPRLGRIW
                     NVAKKEGIPVFHHSCGNVLEILDDMIEIGLNVLIPVQPQAMDIQLLQNRFGDHLAFMG
                     GISTQKTLPFGTPTDVQSEVRNCIEILGSKNNYIISASHEIGSDCPINNLLAFFNEMK
                     KVNGSVIPLLDK"
     gene            complement(2570..3487)
                     /locus_tag="PK171_01025"
     CDS             complement(2570..3487)
                     /locus_tag="PK171_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT04818.1"
                     /translation="MAYSIGIDLGGTKIAGVLVNEQLDILAYEKIPVETERRIEKVLN
                     DIASLAQLLMKKLKKQNELVGMGIACPGLIDINRGVMVYSENLNWRNVPVVQILSEKL
                     NFPFYLEHDVRSGAIAEVFFGAGKKYRNLVYVSVGTGISGTLIWERQFIRGARGISAE
                     LGHIIVEPGGPLCRCGNAGCLEALSSGSAMEREAYHLTKKPVSGAVIMQKALENVSPY
                     TNIVHQAAYYFGIGLANIAQIFDPEAIVLGGGVSESGDFWLKLVESYYVKHQFWSFSL
                     PDLKLGTFKGKASVMGAAGLPFLKKEGLL"
     gene            3993..5147
                     /gene="nagA"
                     /locus_tag="PK171_01030"
     CDS             3993..5147
                     /gene="nagA"
                     /locus_tag="PK171_01030"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOT04819.1"
                     /translation="MSRIGIFGNIVTPQLVVENSMLLIKDKRIAGIIQSNEPELKIDI
                     RYDYSGKYLYPGLIDLHLHGVLGQDTTDGDKNGLQKMAKYLTTCGVTGFLATTVSESY
                     LRLRKAIETVSKLDKIHNSCSKILGIHLEGPYLSEGRKGAHNAKNLRVPDLSEMKEFL
                     EIDPGLIKRITLAPELPGSLELIEYLSKQGVCVSLGHSQADQETCYLAYRKGAHLVTH
                     LFNGMDPLNHRKPNLLSFALGFDEITVELIADMIHIHPDIIKIALKCKDEDHFIAVSD
                     AVRPTGFKDGEYEFGGELMVVEKGIARMKSTMSLAGSTSPLNRCLANLRAHFQISHPR
                     LAKIGSLIPAQLIKMEHQIGSLEINKNADIAIFDDQFNCHATFINGEQVF"
     gene            complement(5173..6636)
                     /locus_tag="PK171_01035"
     CDS             complement(5173..6636)
                     /locus_tag="PK171_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT04820.1"
                     /translation="MSKDNALVMGIDCGTQGIRCLVSDLEGNIVADGQENILSSNKFG
                     DRFEQDPNEWWDKTQICIQKTLRDLKNNRYSSYDIHCFSIDSTSGTIIPIGENGQPLL
                     PAIMYNDSRAKEESLYINRVAKDFTQKIGYQFDPSFALCKVVWLKNHLPEIYDKTKIF
                     LHAGDFLLMKLTGGLFQSDISNSLKMGFDLLEMKWPNFIRDQLEIDIQKLPPVVKTGE
                     ESGIIKASIAKQFQFKPSLKIIAGATDGTASFFASGARIPGDISSTVGTTLVIRGISE
                     KLIKDIKGRIYCHLHPAGYWLPGGASNTGGECLQKFFTNENLKEWDSRVVEMSIPTSL
                     ILYPLTRKGERLPFASPEAEFFQSRKESSRLEFYTACLEGVGYIEKYSYYILEKLGSS
                     PIQRVFSSGSGSNSPIWCQIRSNILSKPIFLPKTTESAMGACIIAASYLHGSLSLASQ
                     KMVKINEVYEPQKTISLQYQEKYHEFLNECQKRGYVH"
     gene            complement(7060..8289)
                     /locus_tag="PK171_01040"
                     /pseudo
     CDS             complement(7060..8289)
                     /locus_tag="PK171_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
BASE COUNT         2650 a         1571 c         1569 g         2542 t
ORIGIN      
        1 aacatttcgt gaaaatcaat taactggtca agaaataacc ttaaatcata tgtcatcagg
       61 agggatgtca tgatgagaac tcgtcgtttg gggaaaacca atctcgagtt aacagtaatt
      121 ggatttgggt cttgggcaat cggtggtgga ggatggagat atgcctgggg tccccaagat
      181 gattcagagt ctatagaagc cataaaaaaa gctttggatc ttgggataaa ttggattgat
      241 actgctgcaa tttatggcct tggacattca gaagaaatag ttggaaaagc gattaagggt
      301 ttttccaaaa aaccaatcat agcaacaaaa tgtggtttgg tatgggatga taagaaaaat
      361 atttccaatc ggctgaaaag agagagcatt aaagctgagg ttgaagcaag tttgaggagg
      421 ttagatgtcg acaccattga tctttatcaa attcactggc caattcccga tgaagatatc
      481 gaggaaggat gggaagcaat ggctcaattg gtaaaagaag ggaaagttag atatattggg
      541 gtatcgaatt ttaatgtatc ccaaatgaaa cgagcccaat caatttatcc aattgcatca
      601 ctacaacctc cttacagcat gttgaaaaga gatattgaag aagacatact atccttctgt
      661 aaccaaaact caattggtgt tgttgtttat agcccaatgc aaaaaggatt actgaccggc
      721 aaaatgactc gggaacggat tgcaagcttt ccggctgatg atcaccgaag aaacgatccc
      781 gagtttaatg agccattaat tagctataat cttgaattcg tgaatcaact gattccaatt
      841 gccaaaaaac ataataaaac tcttgcacaa ctagcaattg cttgggcttt gagacgtcct
      901 gaagtgacgt cagctattgc aggagcccga catccggatc aaatagctga aacggtttta
      961 gcgggcgatt ggacacttcc tcaagaggat attgacaata ttgagaaatt gttccgggag
     1021 cgtaaacaaa aaattatgaa taaataatta caaaaatcat agagggtgaa agcgttacaa
     1081 ttgatccgat aattctgctt cgccctctac cctgaaaaca ccaatattgg tgaaaaaaga
     1141 ataaacaaaa aaagaaaaag gcacaccccc cttagtcccc cccctcaatg ggggaatgaa
     1201 ttcaacaatt cccctccttg gggggtgccg ctttacggcg gggagggtgt tttttcggtt
     1261 tttcctcgat aacctgagaa aattataaag aaatttgatt aaagttgaga tcctcacgcc
     1321 ctcaaaaagc gagggctcag gatgacatcg gtagtgttag atgagattct cacgtcgcat
     1381 aatacgctcc tcagaatgac ggagaaaaat ctccccctct ccccttcaac ctttattttg
     1441 cccgattagt agcgacatgc catggcatgt cgaattttgg gttttcatct tcatctggtg
     1501 ccacaacctt agcatgaggg cttatcatgt ttttatttat ccaatagggg gataacactc
     1561 ccatttactt ttttcatttc gttaaagaaa gccagtaagt tatttattgg gcaatctgat
     1621 ccaatttcgt gtgatgctga aattatataa ttgtttttag aacctaaaat ttcaatacaa
     1681 tttcttactt ccgattgaac gtcggtagga gtgccaaaag gtaaagtttt ttgagtactt
     1741 attcctccca taaaagcgag gtgatcgcca aaacggttct gaagaagttg aatgtccatt
     1801 gcctgtggtt ggacgggtat aagaacattg agtccaattt caatcatatc atccaatatc
     1861 tctaagacgt ttccacagga atgatggaaa accggtattc cctctttttt ggcgacattc
     1921 catattcttc ccaatcttgg tttaagaagg tcttgccagg tttttcgagc aatcaacaaa
     1981 cccctttgag tcccataatc atccccagta tagattccat ctacgccaag ttggatcagt
     2041 tgacgaagaa cctcgatttg gtaatcagta atgagatcca aaagcctttc taattcggtt
     2101 cgattatcaa taagatcaat catgaaattc tcaaaacctc tcaataacca ggctcgttcc
     2161 ataagcaccc aaccctgggt tgagagaata aaatattctt ttttttgcca atcctgaagt
     2221 ggaataaaat cctgatatag aattgaattc tggggatcgg ggaattgaaa ggaattaaag
     2281 tttttccaat ccgttaacgg atgataacaa gggagatacc cttccgattt aacaagatcc
     2341 catccaactc caaaaacatc gtagtcaacc cccttttctt catccaattt tactttttta
     2401 tgggtatcga tatagaggaa atggtttcct aaaagttcag ggagctcctt ttcctggatt
     2461 tgaaaatggt tgagaaattc ttttttcatt ctccaggtaa aatcgatttg atgaggaagc
     2521 cgatcgcttt tttggtgatg gatagcgtga ttgactcttt cttttttggt caaagcaaac
     2581 cctccttttt cagaaatggg agtccagctg cacccataac actcgctttt cccttgaaag
     2641 tcccgagttt caaatcagga agggagaacg accagaattg atgtttaaca taataagatt
     2701 caacgagctt gagccagaaa tcaccggact cggaaactcc acctcctaaa acgatggctt
     2761 ctggatcaaa gatttgagca atgttagcca gaccaattcc aaaataataa gcagcttgat
     2821 ggacaatatt ggtataaggg gaaacattct ccagggcttt ttgcattatt acagcaccgc
     2881 tcaccggttt tttagttaaa tggtaagctt ctctttccat ggcacttccc gaagagagag
     2941 cctcgaggca cccggcgttg ccacatcgac aaagggggcc accaggttca acaataatgt
     3001 ggcctaattc ggcagaaatg cctcttgctc cacgaatgaa ttgtctttcc caaattaaag
     3061 ttcctgaaat tccagtaccg acacttacat ataccaggtt ccgatatttt ttcccagctc
     3121 cgaaaaatac ctcagcgatg gcaccgctcc gtacatcatg ttccaagtag aatggaaaat
     3181 ttaatttttc agaaagaatc tgaacaaccg gaacgtttcg ccagttgaga ttttctgaat
     3241 aaaccataac tccacggttt atatcaatta atcctgggca agcaattccc attccaacta
     3301 actcattctg tttttttagt tttttcataa gtaattgcgc taatgaggca atatcattta
     3361 agactttctc tatccgtctt tctgtttcta ctggtatttt ttcgtaggca agaatatcca
     3421 gttgttcatt tactaatact ccagctattt tggttcctcc taaatctatt cctattgagt
     3481 acgccaaaac ttatcttcct ttctaatatt tttttatgag cttattttta gtgaagtaat
     3541 gaattgtatt gcacttcatc atacataacc gataggattt tattgatgta tcagattgaa
     3601 tatgatgagt tattcctaaa attaatataa caataagagc aataggtata attataaagt
     3661 tacaatttaa atgattacga attcaaaata cccttatggc tgtttttcct ttatttagtt
     3721 ggaaatacct tgataaaatt tggcaaaaac tcagtaaaaa aagaagataa gaaccctata
     3781 aacgaggact aaactgaact attgctaacc aagatccaaa aaaatgaaaa ttagaaaata
     3841 tttagtgata attagcacta atatatcata ttatataaga aagttttgat atccaaacca
     3901 gactttaaaa taaggttttt tcaattttga caatattttc tataaaaact ttgaaattga
     3961 atttttaaca aagaaaaaag gatgatttga aaatgagtcg aattggtatt tttggaaata
     4021 ttgttacccc tcagctagtg gttgaaaatt caatgctctt aattaaagat aaacgaatcg
     4081 cgggaataat acaatcgaat gaaccagaac taaaaattga tataaggtat gattattcag
     4141 gaaaatatct ttatccaggt ttgattgacc ttcatctcca tggtgtttta ggtcaggata
     4201 caactgatgg tgataaaaat ggtttacaaa aaatggcaaa atacctaaca acctgtggag
     4261 taacaggatt tttagctaca actgtttctg aaagttattt acgacttaga aaagccattg
     4321 aaacagtatc taaattggac aaaattcata attcttgttc aaaaatttta ggcatccatt
     4381 tagagggacc ttatttatct gaaggacgaa aaggtgcaca taatgcaaaa aacctaagag
     4441 tgccagatct ttcagagatg aaagaatttt tggaaataga tcccggcttg attaaaagaa
     4501 ttactttagc acccgaactt ccggggagtt tagagcttat agaatattta agtaaacaag
     4561 gtgtttgcgt atctttaggc cacagtcaag ccgatcagga gacctgttat ttggcttacc
     4621 gcaaaggagc acatttagta actcacttat ttaacggtat ggatccactg aatcaccgga
     4681 agccaaattt actttctttt gcccttggtt ttgatgagat aactgttgaa ctcatagcag
     4741 atatgattca tattcatccg gatattatta aaattgcctt aaagtgtaaa gatgaagatc
     4801 actttattgc agtaagtgat gctgttaggc caactggttt taaggatggt gaatatgagt
     4861 ttggagggga attgatggtt gttgagaaag gaattgcccg aatgaaatca acgatgagtt
     4921 tggcgggaag cacctctccc ctcaaccggt gccttgccaa tttacgagct cattttcaaa
     4981 tatcacatcc acgattagca aaaattggaa gccttatccc cgctcagctt ataaagatgg
     5041 aacatcaaat tggtagtctt gaaatcaata aaaatgccga tattgctata ttcgatgacc
     5101 aatttaactg ccatgctacc tttattaatg gtgagcaggt tttttaatta aataagtgcc
     5161 cggcgcataa tattaatgga catatcctct tttttggcat tcattaagaa attcatgata
     5221 tttttcttga tattgaaggg atattgtttt ttggggctcg tagacctcgt taattttcac
     5281 cattttttga ctggcaagcg ataggcttcc atgcaaataa gaagcagcga taatacaagc
     5341 acccatagcg gattcagttg ttttcgggag aaatattggt tttgatagga tgttggaccg
     5401 tatttgacac caaatagggc tatttgaacc gcttcctgaa gaaaaaactc tttggattgg
     5461 ggaagaccct aatttttcca atatataata actatatttt tcaatatatc ctactccttc
     5521 aagacaagca gtgtaaaact cgagtcgact tgattctttc cgagattgga aaaactcagc
     5581 ttcaggtgaa gcaaaaggaa gacgttcccc ctttctggtt aaaggataca aaattagtga
     5641 tgtaggaatg gacatttcca ctaccctact atcccattct tttaaatttt catttgtaaa
     5701 aaatttttgt aaacattcac caccagtatt gctggcacca ccgggtaacc aatagccggc
     5761 aggatgaaga tgacaataaa ttcttccttt gatatctttg atcaatttct cagaaattcc
     5821 acgaataact aaagtagttc caacagtact cgagatatca cctggtatcc ttgcaccaga
     5881 cgcaaaaaaa gaagctgttc catctgtagc tccagctatt attttcaaag aaggtttaaa
     5941 ttgaaattgc ttggcgattg aagcctttat gatacctgat tcttctccgg tttttacaac
     6001 cggaggaagc ttttgaatat cgatttctaa ttgatcccga ataaagtttg gccatttcat
     6061 ttccaaaagg tcaaaaccca ttttaagtga attggatata tcagactgaa aaagaccacc
     6121 ggttagtttc attagtaaaa aatcaccggc atgtaaaaat attttagttt tatcatatat
     6181 ttcaggaaga tgatttttaa gccaaactac cttacataga gcaaaagaag gatcaaattg
     6241 ataaccaatt ttttgggtaa aatccttagc taccctgttg atgtataagg attcctcttt
     6301 tgcccgactg tcattataca taatagcagg aagaagaggc tgaccatttt caccaatagg
     6361 tatgatagtt ccagatgtag aatctatgga aaaacaatga atatcatatg aagaatatcg
     6421 gttatttttt aaatcacgca aagttttttg aatgcaaatt tgggttttat cccaccattc
     6481 attgggatct tgttcaaatc gatctccaaa tttatttgaa gaaagaatat tttcttgacc
     6541 atcagcaaca atattaccct caaggtctga aaccaaacat cgtatgccct gagttccaca
     6601 gtcgataccc atgactaaag cattatcttt actcattttt aaggccacct tttataaaga
     6661 gttattcatc aacatcaaga cccgtaaact ttttcactat ccaagcaaga aaccaatact
     6721 acattttatc ttttctcaaa ttttattaag gtagaatacc ttctttgaaa ctaggtcact
     6781 ttgatagata atcatattct atcctgaaaa tctatatgct ggcatgataa atcaagccct
     6841 acaaaagaat taaaatatgg aggggcacaa taaattgtac ccgactaatg tagcgacatg
     6901 ccatggcatg tcgaatctta ggttttatcc ctcatctggt gctgcaaagc atcatgaagg
     6961 tctatcctta ttatcgcatg tttctataga aagaataatt gaaataaata tttttagttc
     7021 gtgaagtact tagttatttt tattgaaaaa ttaaaatcct tacgtcgagg atttttcttt
     7081 atgcaggatt tttactgtat ctgaatgaag aaatatctcg actacccgag gatcaaacat
     7141 ttttccactt tgtttacgaa tgtaatcaag agctttttta actggccagg catcacgata
     7201 agggcggttg ctggttaagg catcaaagtt gtcggcaacg gcaaaaattc gagccgaaag
     7261 gggaatagct tcgcctttta agcctcgagg gtacccgctc ccatcccacc tttcatggtg
     7321 acaataaggg atgtcgatag ctgggtgtaa atattgaatg ggtgaaagca tctcataggc
     7381 ataaatcgga tgcttcctca ttatttccta ttcttcattg gtaagctttc ctggtttaaa
     7441 aagaattgag tcatgaatac ccattttacc gatgtcgtga agaagcgctc ctcgacgaat
     7501 gtggactaaa tccttttctt ctatccccat ttctcgagct attttgaggg ttaattctgt
     7561 tactcttttg ctgtgattct ccgtttccct atctttgaga tctaaagcat aagcccatcc
     7621 ttcaatggta gcatcatagg tttcgatcag caaagcattg gttttttcca agttgtaaac
     7681 taattcttct ttttcgacgg caattaccac ctgttcagcc aagcttctca aaaaatcgta
     7741 ccattcctca ttcccaatat ggagggagcg attaaaaatt tctaagaccc caaacaatcg
     7801 ccctttggct aaaagaggca cagcataata tgaaacaaag ctttcatccc ggaaaagttt
     7861 ggtttgatag taagtactat gttcgctcac atttggaacg gagatgattt gacgttccaa
     7921 agcagcttgg ccagcaaaag cgtcgcctaa tttcaaggag gttttttcaa tatcagaagt
     7981 gcgaaaacca attcccgcag catagactaa agtttgttgc tgggggtcat agcgcaataa
     8041 agaagcagca tcaacttcta accgttgaac aatctgctcc aaggcaactt tttgagccag
     8101 tattggttca ctgcttcctg aaattgccat atcgatggta cgcaaagctt ggatatgttt
     8161 gaggcgttgt tcggtttctt caaataaacg ggcattttct aaggatgaag cggcatgatg
     8221 agctatggtt tggaaaagtt ctaagcgctc tggatgaaag aaacccggag ttttactgta
     8281 tagattcaac gttccaaaag tccgaccacg actgatgagg gggaaagcag ca
//
LOCUS       DAOSOL010000021         8125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_8820_length_8125_cov_4.961338, whole genome shotgun sequence.
ACCESSION   DAOSOL010000021 DAOSOL010000000
VERSION     DAOSOL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8820_length_8125_cov_4.961338"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PK171_01045"
     CDS             complement(<1..557)
                     /locus_tag="PK171_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04821.1"
                     /translation="MINSREFWNEAKSSFRLLISRPSFILTTAAPALIFSLILYMTPG
                     RRLVWISSYLQSFTLAGLGIAGMFTVFLGMSFLIALVWDYQYRNLIDFRRVYRIIQSR
                     FSDVLLACLGLGLLIGFFSVWFVFAGFFLAFLLIFCLPAIVISGDDPFSAIKTSFRMV
                     YENLAEVFAFFILALALLVIGYFLV"
     gene            698..1759
                     /locus_tag="PK171_01050"
     CDS             698..1759
                     /locus_tag="PK171_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT04822.1"
                     /translation="MRNFFMIWFLILILLLLFCPTSFALDAFDQAQIYLESGDIDRAI
                     QILKPLTNSSNEDELSQVVEVLYTLFSEKGQNQDAIHVLQIFIEKFPRTQSAYLYRYW
                     IAKTEEDNKKYQESLKLLQKIVAEYPTDIGDPFNIRQQAMEDIAHHQENYFGNYTEAI
                     NSYLTILSQYPDFEEKSRILLQVASCYEKMNQFDKAMEFYQKIRFQETDPYYLDLSEL
                     RIEYLQSDPTWTRKNSAILIKELGDAFAKKDLQAIESLAKKGDFWTGQMFSEFEIVRF
                     SQIIPYFSTYLTQSTLQIHPAEKKENEYYILKITSWGDPDFPILYLYIEKGIYGWEWS
                     KIVLSNPEIECQVNNFDDT"
     gene            2029..2613
                     /gene="hisB"
                     /locus_tag="PK171_01055"
     CDS             2029..2613
                     /gene="hisB"
                     /locus_tag="PK171_01055"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOT04823.1"
                     /translation="MQKREALKKRTTFETDIEIYILLDGKRQIELDLPIPFFPHLLKS
                     MAFYADWDLNIKARGDVEVDFHHLVEDIGIVLGEAFRTALGETKNIQRYSTQFIAMDE
                     ALSMVSIDVGGRSFLNYDVSIGSQKVGDFEADLIEEFLRAFTNHAHITLHGKSWWGKN
                     GHHILESLFKALGKAFKEALILNGNTIPSTKGII"
     gene            2627..3235
                     /gene="hisH"
                     /locus_tag="PK171_01060"
     CDS             2627..3235
                     /gene="hisH"
                     /locus_tag="PK171_01060"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOT04824.1"
                     /translation="MIVIIDYGIGNLHSVSKALEKLGFENQISKDPDLVEKAPALILP
                     GVGSFGEAVEQMEKNNLIDVICNSVQKGKPILGICLGLQLLFEKSQESTKKKGLSFIE
                     GEVRKLPPTNKVPHMGWNKILFTENNPLSGLIPDGRFFYFAHSYYVVPESKESIVGIS
                     NYNVVIPVIINQDNIWGVQFHPEKSSKWGIHFLETWAKRVIP"
     gene            3232..3981
                     /gene="hisA"
                     /locus_tag="PK171_01065"
     CDS             3232..3981
                     /gene="hisA"
                     /locus_tag="PK171_01065"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOT04825.1"
                     /translation="MILPIPAIDIINGQCVRLEKGDYSQMSIYSDSPLDMALYWEGKN
                     APMLHLIDLDGAKIGKPVNLSIFSKIIQSVHIPVEVGGGIRSLSTFLSYLDAGAQRLI
                     FGSVAVQNPKLVEDCIKRSQESVVVSIDSRNGLVALQGWTEKTTISAVDLTNKLKNLG
                     VINFIYTDIEQDGTLQGVNLNRIQKYLQETEVPTFIAGGVATFEDVLQLKKLKPWIKG
                     IILGKALYSGALQFEEVQKILQEEEKCSLKE"
     gene            3963..4745
                     /gene="hisF"
                     /locus_tag="PK171_01070"
     CDS             3963..4745
                     /gene="hisF"
                     /locus_tag="PK171_01070"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOT04826.1"
                     /translation="MLVKRIIPCLDVKEGKVVKGIHFENLKDVGDPVELAKRYEEEGA
                     DELVFLDITASYEKRETMVKIAEKVAQNIYMPFTVGGGISTHEHVSNLLKAGADKVSI
                     NTAAVLNPDLISQLAEKFGSQCVVVAIDVKKTWDRITRRSYWEVYINGGRTPTGKDAI
                     AWASQVESLGAGEILLTSMDTDGTQNGYDLFLTRKIVDGVKIPVIASGGAGKLEHLLT
                     VFREGGADAALVASIFHYKNFTIQEAKAFLKKRGIPVRIEEP"
     gene            4742..5401
                     /gene="hisIE"
                     /locus_tag="PK171_01075"
     CDS             4742..5401
                     /gene="hisIE"
                     /locus_tag="PK171_01075"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOT04827.1"
                     /translation="MMKSLIENLHFDENGLIPAIIQDEKSGKVLMMAYMNAEALEKTI
                     TTGKTWFYSRKRKTLWNKGETSGHYQLVKDIFIDCDQDTLLITVEQKGAACHTGFNSC
                     FHRQVSEKGISSTQSELPPYALASFPQELFDVIQERAKNPSAQSYTSKLLQSGKEKIL
                     RKVSEEATEVLLASLENDEQKKEHLQWEIADLLYHLLVLIQHEKLNWYDIMNELKKRR
                     K"
     gene            5768..5842
                     /locus_tag="PK171_01080"
     tRNA            5768..5842
                     /locus_tag="PK171_01080"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5800..5802,aa:Ile,seq:cat)
     gene            5868..7001
                     /locus_tag="PK171_01085"
     CDS             5868..7001
                     /locus_tag="PK171_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT04828.1"
                     /translation="MATLQQLLDFFEYLYPSHFALTDDRTGLQIKGDRSQISRVLVAL
                     ELNQEVFLKALKHRIDFLYLHHPPIWNPLFTLTIDDPWCRMITRLFHEGISVLAHHTN
                     LDIYPGGIADQWVSILQLQGKVKSLSPKSSASFKIITYVPPNYLETVAQSLFQSGAGV
                     IGQYRSCSFQIEGIGTFLPIEGAQPFLGKVGNREKVKEIRIEVTFSDPRLINQLVDAI
                     KSTHPYQNPVIDIYPLCSNSSVFEGLGRLVVLNQPLKWKSFIKYLKPLLKNESSLFNR
                     KDFGNSTLQKIALCPGSGGSLLDKVILEKAEVYISGDLSHHEIEELKLYQIEYIPVPH
                     GDGERLGLKMIFANLKNSALEHLPEVVFLFEDELDGEDSKISG"
     gene            7109..7807
                     /locus_tag="PK171_01090"
     CDS             7109..7807
                     /locus_tag="PK171_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOT04829.1"
                     /translation="MLNIFECLTHLQNLDGIILNQKKKITQKNEEIRKTQQNIENKKI
                     EWEDYRKVYDKERIELSRLELELKDVEEKLNKSQNRLYSGDVQSGKELSQWKSTMENY
                     EKTKSNLEDYVINQIDKIEEMRIKEKEKEHQLFIQRFEKDIVSLKKDIEKIKSDLANN
                     IQLRTEAIQNIPEEVLSLYEELRKKFSDPVVEMDGDTCRGCHLGLPSSVAKRVRKKEE
                     IIQCPNCQRLIFYK"
BASE COUNT         2699 a         1539 c         1560 g         2327 t
ORIGIN      
        1 caaaccagga aataaccaat cactaataat gccaaggcaa ggatgaaaaa tgcaaatact
       61 tcagccagat tttcataaac catacggaaa ctggttttta tggcactaaa agggtcgtct
      121 ccgctaatca ctattgccgg taaacaaaaa attaataaga aagccagaaa aaaaccagcg
      181 aaaacaaacc aaaccgaaaa gaatccaatt aataatccca gacccagaca agctaaaaga
      241 acatccgaaa atcggctctg gataatgcgg taaacccgtc gaaaatcgat cagattacgg
      301 tattgataat cccagaccaa agcaataaga aaactcatcc cgagaaacac ggtaaacatt
      361 ccagcaattc ctaaaccagc cagagtaaat gattgtaggt aggaggatat ccataccaac
      421 cgtctccccg gggtcatgta tagtataaga gaaaaaatca atgccggcgc tgctgtggtg
      481 agaataaacg atgggcgact gattaaaaga cggaaactac tcttggcttc attccagaac
      541 tctcggctat taatcatgtt cttctcctat gggaaaatat tcgactcagt tcatattact
      601 atcattcatg ggtcatgaca agttttttac cgagaaaatt tgagttgatg gtataatacc
      661 aaaaagaagc atgccaacca aaaaaggggg atgtgttttg agaaattttt tcatgatttg
      721 gtttttgatc ttaatccttc ttctgctctt ttgtccaacc agcttcgctt tagatgcttt
      781 tgatcaagca caaatttatt tagaaagtgg ggacattgac cgagccattc aaattttgaa
      841 acctttaacc aattcttcaa atgaagatga gctctctcag gtggttgagg ttttgtatac
      901 tcttttcagt gaaaaaggcc aaaaccagga cgcgattcat gttctccaaa tttttatcga
      961 aaaattcccc cgaactcaat cagcctattt gtatcgatat tggatagcca aaaccgaaga
     1021 agataacaaa aaatatcagg aatctttgaa acttctacaa aaaattgtcg ctgaatatcc
     1081 caccgatatt ggtgatcctt ttaatatccg tcagcaagcg atggaagata tagctcacca
     1141 tcaagaaaat tattttggaa actacactga agctatcaac agttatttaa ctattttatc
     1201 ccaatatcct gattttgaag aaaaatcgag aattctatta caagttgctt cctgctatga
     1261 aaaaatgaat caattcgata aagccatgga attctatcag aaaattcgct ttcaagaaac
     1321 cgatccttat tatcttgatc tgtctgaatt gagaattgaa tatcttcaat cggatccaac
     1381 ttggacaaga aaaaattcag caattttaat caaagagctt ggagatgctt ttgctaaaaa
     1441 agaccttcag gctatcgaaa gtttagctaa aaaaggcgat ttttggacgg gtcaaatgtt
     1501 cagtgaattt gaaattgtga gattttccca aattattccc tatttttcaa cctatttaac
     1561 tcaatctacc ctccaaatcc acccggctga aaagaaggaa aatgagtatt atattctaaa
     1621 aatcacctct tggggcgatc ctgactttcc aattctttat ctctatatcg agaagggaat
     1681 ttatggatgg gaatggagca aaattgtcct gtccaatcct gaaatcgagt gtcaggtaaa
     1741 caactttgat gatacctaac ctgaaataaa tttgatatta ttcatttttt ttgtaaaata
     1801 gaaggagtaa cttaaagtca gctaataatt atagaataca tattgattag ataatgatat
     1861 atcagccctc gaaacaccaa gctgaatcct cctcatgtta gaggagttga aggtaacaat
     1921 tcctctgggg gaggtttcat gccaccttct gattaaaaaa ggttattaac tttaatatat
     1981 tgttctattc cacaatatac atatatttat taccaatgag gtgattttgt gcaaaaacga
     2041 gaagccttaa aaaaaaggac aacctttgaa accgatattg aaatatatat tcttcttgat
     2101 ggtaagcggc aaatcgaact tgaccttccg attccttttt ttcctcattt attgaaaagt
     2161 atggcatttt atgctgattg ggatctcaat ataaaagctc gtggtgatgt tgaagttgat
     2221 tttcaccatc tggttgaaga tattgggatt gtgttgggcg aagcctttcg aactgcgctt
     2281 ggggaaacaa aaaatattca acgttacagt actcaattca ttgccatgga tgaggctttg
     2341 agtatggtct ctattgatgt tggtggtcga tccttcctta attacgatgt ctcaattggt
     2401 tctcaaaaag ttggtgattt tgaggctgat ctgatagaag aatttctaag ggcttttacc
     2461 aaccacgcac acattactct tcatggcaaa tcatggtggg gtaaaaatgg acaccatatc
     2521 ttagagtctc ttttcaaggc tttaggaaaa gcttttaaag aagcactgat attaaatggt
     2581 aatacaattc cctccaccaa aggaatcata taggagaata ttaaaaatga ttgttattat
     2641 tgattatgga ataggtaatt tacacagcgt atcaaaagct ttagaaaaat tagggtttga
     2701 aaaccaaatc agcaaagatc cggatttggt tgaaaaagct cctgctctta tccttcctgg
     2761 ggtcggatct tttggtgaag cggttgaaca aatggaaaaa aataacctca ttgatgttat
     2821 ctgcaattca gttcaaaaag gaaagccaat tttaggcatt tgtttagggc tgcaattgct
     2881 ttttgaaaaa agtcaagaat cgacaaagaa aaaagggtta tcattcatcg agggagaggt
     2941 acggaaactt cctccaacca ataaagttcc tcatatgggg tggaataaaa ttctttttac
     3001 cgaaaacaac ccgctctcgg gattgatacc cgatggccgt ttcttttatt ttgcccattc
     3061 ttattacgtc gtaccggaat cgaaagaaag tattgtggga atcagcaatt ataacgtggt
     3121 tattccggtc attatcaacc aagataatat ctggggcgta caatttcatc cagaaaaaag
     3181 ctcaaagtgg ggaatccatt ttcttgaaac ctgggcaaag agagttatcc catgatttta
     3241 cccattccag ctattgatat tatcaacggg cagtgtgttc gcctggaaaa aggcgactac
     3301 tcacaaatga gtatttacag cgattcccct cttgacatgg cgctgtactg ggaaggaaaa
     3361 aacgctccga tgcttcacct gatcgattta gacggtgcaa aaattggcaa accagttaat
     3421 ttatcaatat tttcgaagat cattcaatcg gttcatattc cagtagaggt tggtggtggt
     3481 atccgtagtc tttccacttt cctctcctat ctcgatgctg gtgctcaacg gttaatcttt
     3541 ggaagtgtcg ctgtccaaaa tcccaaactt gtcgaggatt gcatcaaaag aagccaagaa
     3601 agcgttgtag tgagcattga ctcccgaaac ggtttggttg ccctgcaagg ttggaccgaa
     3661 aaaaccacca tatccgccgt cgatcttacc aacaagttaa aaaatttggg tgtgatcaat
     3721 tttatttata ctgatattga gcaggatggt actcttcagg gagtgaacct caaccgtatt
     3781 caaaaatacc ttcaagaaac tgaagtccca actttcattg ccggaggagt tgccactttc
     3841 gaggatgtgt tacaacttaa aaaattaaaa ccttggataa aaggtattat cttaggaaaa
     3901 gcactctatt cgggtgcatt acaatttgaa gaagttcaga aaattcttca ggaggaagaa
     3961 aagtgctcgt taaaagaata atcccctgtt tagatgtcaa agaaggaaag gttgtcaaag
     4021 gaatccactt tgaaaatctc aaggatgtcg gcgatccggt cgagctggcc aaacgctacg
     4081 aagaagaagg tgctgatgaa ttagtatttt tagatatcac cgcatcctat gaaaaaagag
     4141 agaccatggt gaagattgct gaaaaagttg cccagaacat ctacatgccc tttaccgtag
     4201 gtggtggaat cagtacccac gaacatgtca gcaatcttct caaagcagga gccgacaaag
     4261 tatctatcaa tacggcagca gtgctcaatc ccgatttgat atctcaactg gctgaaaaat
     4321 tcggaagtca gtgtgtcgta gttgccattg atgtcaaaaa aacctgggac cgcattaccc
     4381 gccgcagcta ttgggaagtc tacattaatg gagggagaac accaaccggg aaagatgcca
     4441 tagcctgggc aagtcaagtc gaaagcctgg gcgccggtga aattcttctc accagcatgg
     4501 ataccgacgg aacacaaaat ggttatgatt tatttctcac caggaaaatc gttgatggag
     4561 taaaaattcc agtaatcgct tctggtggag ctggaaaatt agaacatctt ctgacggttt
     4621 ttcgtgaagg aggagccgac gctgcactgg tcgcttcaat tttccattat aagaacttta
     4681 ctattcagga agctaaagcc tttcttaaaa aacgtggaat cccagtaagg attgaagagc
     4741 catgatgaaa tccctgattg agaatcttca ttttgatgaa aatggcttga ttccagcaat
     4801 catccaagat gaaaaaagtg gcaaggtttt aatgatggcg tatatgaatg ccgaagcttt
     4861 ggaaaaaacc atcaccacag gtaaaacctg gttttatagt agaaaaagaa aaaccttatg
     4921 gaataaaggg gaaaccagcg gccattacca actggtaaaa gatattttta tcgattgcga
     4981 ccaggatact ctcctgataa cggttgagca aaaaggagct gcttgtcata ccggattcaa
     5041 ctcttgtttc catcgccagg tttcagaaaa aggaatatct tcaactcaat cggaattgcc
     5101 tccttatgca ttagcatcat tcccacaaga actttttgat gtcattcaag aacgagctaa
     5161 gaacccatca gcacagtctt atactagtaa gcttttgcaa tcgggaaaag aaaaaattct
     5221 acggaaagtt tctgaagaag cgactgaagt cctgctggct tcattagaaa atgatgaaca
     5281 aaaaaaagaa catttacaat gggaaatagc tgatcttctc tatcaccttt tggtcctcat
     5341 ccagcatgaa aaattaaatt ggtatgatat tatgaatgag cttaaaaaaa gacgaaagta
     5401 atcaagaaag aattcaaatt tccctcctcc cactcgctct cgatcttttt ctcctggtgg
     5461 gagaatactt tcttcttttt tcctctcccc tggtgggaga ggattaaggt aagggggaac
     5521 ttggataatc tccttgagct atatttcaaa acactaaacc tggatctcaa tcacaggtct
     5581 caccctcatc ctgaccttct cccatcaagg gagaaggaat agacaaaaca gagtctgaat
     5641 gaattataat attaaaaaat ggaatatata attcattctt taatctattg aataggtatc
     5701 tcttctttaa agttcgaaaa tctcttgaat tgaaaaaaac gatatgatat tatgtatgcc
     5761 gtcgcgtggg tccatagctc agcggtagag cagtcggctc ataaccgatc ggtccctggt
     5821 tcgaatccgg gtgggcccac caatttaaaa gaattaacaa taaccctatg gcaaccctac
     5881 aacaattact tgattttttt gaatatctat atccgtccca ctttgccctg actgatgacc
     5941 gaaccggcct tcaaatcaag ggcgaccgga gtcaaatttc ccgagtttta gttgcactgg
     6001 aactcaatca ggaagttttt ctcaaagccc tgaaacatcg aattgatttt ttatatctcc
     6061 atcaccctcc aatctggaat ccattgttta ctttaaccat tgatgatccc tggtgccgga
     6121 tgataactcg actttttcac gaaggaataa gtgttcttgc ccaccacacc aaccttgaca
     6181 tctaccctgg aggaattgct gatcagtggg tatcgatcct tcagctccaa gggaaggtga
     6241 agtcgctttc cccaaaatcg agcgccagct ttaaaatcat aacctatgtc ccacctaatt
     6301 atttagaaac tgtcgctcaa tctctttttc aaagtggtgc tggggttatc ggtcaatatc
     6361 gatcgtgtag ttttcagatc gaaggcatag gtacttttct tcccatagaa ggagctcaac
     6421 cttttttagg aaaagttgga aatagggaaa aagtcaaaga aatccggatt gaggttacct
     6481 tctcagatcc gagactcatc aatcagttgg ttgatgccat taaatcaact catccctacc
     6541 aaaatccggt aatcgatatt tacccactat gctctaacag ttctgttttt gaaggcttag
     6601 gtcgtttagt tgttttaaat caacctttaa aatggaaatc ttttattaag tacttaaaac
     6661 cgttgttaaa aaatgagtcc tctcttttca atcggaaaga ttttggaaac tctacccttc
     6721 aaaaaattgc cttatgccct ggtagtggtg gaagccttct tgataaagtg attttggaaa
     6781 aagcggaggt ttatataagt ggtgatttaa gtcaccatga aattgaagag ttaaaactct
     6841 atcaaattga atatattcca gttcctcacg gagacggaga aagactcggg ttaaagatga
     6901 tctttgcaaa tcttaaaaac tctgctcttg agcacttgcc tgaggtagtg tttctttttg
     6961 aggatgaact agacggagaa gactccaaaa tctcgggtta aatacgaaaa cttaatagta
     7021 gtaccagcag ttttttgtag attttattga caaaaccata gattaaccat cacaataaag
     7081 gaagattttt taccttagga ggttgccttt gctcaatatc ttcgaatgcc taactcatct
     7141 ccaaaatctc gatggaatta tcctcaatca aaaaaagaaa attacccaaa agaatgaaga
     7201 aattcgaaaa acacaacaaa atatcgaaaa caaaaaaata gagtgggaag actaccgaaa
     7261 ggtatatgac aaagaaagaa ttgaattatc tcggttagag ctggagctta aagatgttga
     7321 ggaaaaatta aataaatcgc aaaatcggct atatagtggc gatgttcagt ctgggaagga
     7381 actgagccaa tggaagtcga ccatggaaaa ctacgaaaaa accaaatcta atcttgaaga
     7441 ttatgtcatt aatcaaattg ataaaatcga ggaaatgaga attaaagaaa aggaaaaaga
     7501 acatcagctt ttcattcaac gatttgaaaa agatattgta agtctaaaaa aagatattga
     7561 gaaaattaaa tctgaccttg ccaacaatat tcagctgaga accgaggcca tccagaatat
     7621 ccctgaagag gtcctttctc tatatgagga gttacggaag aaattttctg atccagttgt
     7681 ggaaatggat ggtgatactt gccgaggatg tcatttggga cttccctcct cggttgccaa
     7741 gagagtaaga aaaaaagaag agattattca atgtccaaac tgccaacgtt taattttcta
     7801 taagtagttt ttgaagaatc cattttcttt tccgcttaat ctcaagaaaa ctggtttgtc
     7861 tatattgatc ataaatttct tcttcgatat agtcttgatt ttcaagagaa accctcctac
     7921 caattggtgg tatcagataa aaaataaaaa catatactca ataaccaaat cccgaaccat
     7981 ctccctttat ccaaagggag agatctttat gcggatttct ctttttcgta atttcgtaat
     8041 tcataaattt tattcaactc acgactcaca tctcacgact taatggattt atggatagtg
     8101 tataataata aagggaaggt caggc
//
LOCUS       DAOSOL010000022         8070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_8904_length_8070_cov_3.718278, whole genome shotgun sequence.
ACCESSION   DAOSOL010000022 DAOSOL010000000
VERSION     DAOSOL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8904_length_8070_cov_3.718278"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PK171_01095"
     CDS             <1..345
                     /locus_tag="PK171_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04830.1"
                     /translation="ISSSSFYKEKNKKMRLPRHFVPRNDGAGEIATSHNTLLAMTEQV
                     DGIPTSLCSSECHPLASSPPPQSILHPYLVPPSGLVVYLNIFFYSPMLHYCFFRYPSF
                     FQLFSFDLVNKE"
     gene            455..1951
                     /locus_tag="PK171_01100"
     CDS             455..1951
                     /locus_tag="PK171_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT04831.1"
                     /translation="MITTITERIREMSQEYGKKIAVSMGDWFINYNELVNEIDKEALK
                     LKKAGVKEGMRVVIVLPNHWHTLRLILGCFQIGAIPLLLSPKYPPKSIEKIFQNAKAS
                     LLVAQSPHQELADRLPLAIIGENGKIGFPDKINTTINDLPSLDSPIAAFFSTSGTTGI
                     PKLVMLTHKNILSDIDSCFDLVDIYPEDRMLGVLPMFHVFGFSIAYMLPLAKGMTLTI
                     VPSLYPANNLIESLKKYQSTVFLGVPAVFSILAGARAKVPFDLSPLRLPICGGDALPQ
                     RVREAFEKGFGLNIMEGYGITEASPVVSINPSPLTRVPGSAGPVIEAINLRIVDDNGN
                     ELPTGEIGEIILSGDPISPGYYNNPEENQTSFRNQWFYTGDLGKIDEKGLLYIEGRKK
                     ELIIVSGFNVFPQEIEEVIMSFPGVVRTAVVGVQRASRGEMIKAYVVPNENITIDPTE
                     IVRFCRKELASHKVPRIVEIVAELPQTVTGKVMKYMISSSGNEIEVIE"
     gene            2431..3132
                     /locus_tag="PK171_01105"
     CDS             2431..3132
                     /locus_tag="PK171_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOT04832.1"
                     /translation="MNKTKKFLLLSLILTVLFSLGPIARANDEIIDQSIEALHDLSKI
                     PERDVFFYLLEKAEAIAIFPKVVRLGLGLGAQYGDGLVYRKEFNKNNWYGPAFYKIYG
                     VSFGPQVGIQSTSLILLIMNQDGMEIFYNDGLTLGGNVSVAAGPIGRSFSAEVDLNLD
                     SSIYSYSQSRGLYIGLSVQGAQVKQNRQANRQLYKESIDPEEILMSKVSSNPASLRLL
                     QMIKDLITKNSVDDY"
     gene            3147..3356
                     /locus_tag="PK171_01110"
     CDS             3147..3356
                     /locus_tag="PK171_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04833.1"
                     /translation="MRTILSILGVIFLIILAFRFFPVIIAGVLVVIGAILLILLLTGV
                     AIIAAPILILLLLIGVVVWVLRLIF"
     gene            3426..4397
                     /locus_tag="PK171_01115"
     CDS             3426..4397
                     /locus_tag="PK171_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOT04834.1"
                     /translation="MNVGYFCSYIPEEIIQSCNATPVFLSSDSLATTQAIPYLPTPFC
                     PFSKILLNSLLAEKNLELNFIIFGGGCDAGRKLYDIFIALQPKTPCHYLHIPLVSNQE
                     SLKFYRHSLNDLANKLLSFQGISQYNFIENLRIILDHSFTVKQTRSQTFLTGELPGDS
                     LLWDKIQLQSSLDSTISSKISILLLASHLFVENIIQLLEEKEFRVFDGSATGMRRYIF
                     PDIEYTPEHHALDTLARWYLVNKVPCPGYNPQKRLGILQKFINRVGIQGIVYFYPKFC
                     DQSLYDLSFLKKQIKIPLLPVEHDMSYSSIGQWETRIDAFREVLLSQ"
     gene            4394..5629
                     /locus_tag="PK171_01120"
     CDS             4394..5629
                     /locus_tag="PK171_01120"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HOT04835.1"
                     /translation="MNLKASTALSLARFFANRKSLFHLLAIYEWWLKRKFPEPTLPQT
                     SYQLTIKTIRELYSHSRPVIWSSLFFPCEFIHAVGAIPFYPEITIGLVAALGFSNLPL
                     EFAEKNWYSQDLCSYHRAGVGMSLFKLFPRPDFLLATTSICRGTAGFFQSLANIWKIP
                     CYLIDVPYQMTPEAINYVQNQLSSIATLISSQINCQLDWEETFSLSNATVKIIHQIET
                     LRKQKSFAIQPPTKNLDYLPYYFQFLGSPASKFFFEKFLEHLQKLKNPSTNSHRLIWL
                     HLKPFYPNQLTALLNEYHFDVVYDEFASVFWEPLEPEKPFESLAKKIISSQNLTVPEK
                     RILRILNWCEQFKADGVIQFNQWGCRQSQGMNFLLKNTLQQKGYPVLLLDGDHIDKKF
                     YSEEQLRTRIEAFHEILEG"
     gene            5635..6414
                     /locus_tag="PK171_01125"
     CDS             5635..6414
                     /locus_tag="PK171_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT04836.1"
                     /translation="MVFAGLDIGSQSTCATIINEKEIMASVLMPSSVNPKKIAEEALN
                     LALGKANLSRSDVQYFIATGYGRYQAEANEKISEITCQARGVQWFIPEARMVLDIGGQ
                     DSKIILLGKNGRVIDFMMNDKCAAGTGRFLELMTQVLGIQIEDYGTLVNQSREFIELS
                     NTCAIFAESELVSLIAQGKKKEDLARAVCRSVVKRILILAGKMNITRPLVFTGGVAKN
                     QGIITILKEELAFSPLIPPDPLITAALGAACLAREKALPEP"
     gene            complement(6436..7701)
                     /locus_tag="PK171_01130"
     CDS             complement(6436..7701)
                     /locus_tag="PK171_01130"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT04837.1"
                     /translation="MHWLWDEAWIRPGVAIFIVYLFIYWAYQIFIAVRGAWSPPPLPP
                     AKSFHRFAVLIPAHNEEKVVGNLLESLIKQNYPQNYFDIYVSCDNCQDKTAEIAKSYN
                     VNVLEKHDTLHIGKTGNVQWALREIPLENYDAVAMFDADNLVEKNFLAKMNDYMESHP
                     KAQAIQGYLDTKNPDDTWISRSYALSYWYTNRFWQLARSNWGLSAALGGTGLVVTVSC
                     LKTIGWDLKSLTEDLEFSTRIILSGSRVHWNNEAIIFDEKPLTFWASRRQRTRWMQGH
                     YWVLMKYGPQLLLQWLKTWKIQYLDWLFYLLSAVTTVVGFTILAVRIAIGSSWNFGIS
                     IWPLWLASGLIQCAFNVVMGPSFYWGKFTLRYIPDILSYFLYGLTWPPIMFYAAFLSR
                     NQSQWVKTDHTRDIGVSDVRAKEKALNIM"
BASE COUNT         2508 a         1628 c         1433 g         2501 t
ORIGIN      
        1 atctcatctt caagttttta taaagaaaaa aacaaaaaaa tgagattgcc acgtcacttc
       61 gttcctcgca atgacggggc gggtgagatt gccacgtcgc ataatacgct cctcgcaatg
      121 acggagcagg tggatgggat tcccacatcg ctctgctcct cagaatgtca ccccctcgcg
      181 tcctctcctc ccccgcagtc cattttacat ccttatctgg tgccaccaag cggtttggtt
      241 gtctatctta atattttttt ctattcacca atgttacatt actgcttttt caggtatcct
      301 agttttttcc aactgttttc ttttgattta gtcaataaag aataacgaaa tggtagcaga
      361 aataagaaat atagaataaa atacataaag aaatttattt tatacggtat ttaaattaag
      421 atggttactc tatataagct ttgaggagga aagtatgata actacaataa ccgaaagaat
      481 tcgagaaatg agtcaggaat atgggaaaaa aatcgccgtt tcaatgggag actggtttat
      541 aaattataat gaactggtta atgagattga caaagaagct ttaaaattga aaaaagctgg
      601 cgtaaaagaa ggaatgcggg ttgtcatcgt acttcccaat cattggcata ctttaagact
      661 catcctcggt tgttttcaaa ttggagcaat tcctctgctt ctctcaccaa aatacccacc
      721 taaatcaatt gaaaaaattt tccaaaacgc caaagcaagt ttgttggtag cccaatcacc
      781 acatcaagag cttgccgatc gactccctct tgctattatt ggagaaaatg gaaaaatcgg
      841 attccctgat aaaataaata caacgattaa cgatttaccc tctttagact cacctattgc
      901 cgctttcttt tcaacttcag ggacgactgg aattcccaaa ttggtcatgc tcactcataa
      961 aaacatcctt tcggacatcg atagttgttt cgatttagtc gatatctatc ctgaagatcg
     1021 catgttgggt gtacttccca tgttccatgt ttttggtttt tcaattgcct acatgcttcc
     1081 tttagcaaaa gggatgacac ttacgattgt tccttcactt tatccagcca acaatctcat
     1141 tgaatccctc aaaaaatatc aatctacggt ttttttaggt gtaccagctg tcttttcaat
     1201 attagccggt gcccgtgcta aagttccctt tgacttaagt cctctccgcc tcccaatttg
     1261 tggtggtgat gctcttcctc aaagagttcg agaagctttc gaaaaaggtt tcgggttaaa
     1321 tatcatggag ggatatggaa taaccgaggc atctccggtg gtttctatca acccctctcc
     1381 ccttactcga gtaccagggt cagcgggacc agtaatcgaa gctattaacc ttcggattgt
     1441 ggacgacaac ggcaatgaat tacccaccgg tgaaattgga gaaattattc tttctggaga
     1501 tcctatttca cccggttatt ataataatcc tgaagaaaat caaacctcct tccgtaatca
     1561 atggttttat accggagact taggaaaaat tgatgaaaaa ggtcttcttt atattgaagg
     1621 cagaaaaaaa gaattgatta ttgtttctgg ttttaatgtt tttccccaag agattgaaga
     1681 agtaatcatg tcattcccag gagttgttcg taccgctgtt gtaggggtcc aaagagcatc
     1741 tcgtggtgaa atgatcaaag cttatgtcgt tccaaatgaa aatattacca ttgacccaac
     1801 tgaaattgta aggttttgcc gaaaagagtt agcatctcac aaagttcctc gtattgttga
     1861 aatcgttgcc gaacttcctc aaaccgtaac aggaaaggtc atgaaataca tgatttcttc
     1921 ttccggtaat gaaattgaag taattgagta aagtctttca ctctaaatcg gaactaattc
     1981 gaattaaaag aaaagcttat tctgaaaata tagtaatgaa taaaaagtta acaaaaaaaa
     2041 atgaaaaatg tatacccctc cttttgaatt ccctttcaat agggtaatga attgattgaa
     2101 caattcccct cctcgaaggg gtggcgcttg cagaggggag ggtgcttttc gtttttttct
     2161 ggttatcctg agaaaataat aaaggaataa aaaatatttt ctaaatgaga ttgccacgtc
     2221 gttcaaccaa agcacgattg gcctcctcgc aatgacggat ttggtggatg agactctcac
     2281 atcgtctggt aaaagacacc ggactcctca gaatgacaga gtgagtgagt gggattctca
     2341 tgacgccaaa tacgctcctc agaataacag cgaggttgga aaatatggta atattaatcc
     2401 ctcttaaatt gggaaaaagg tggtaaggca atgaataaaa ccaaaaagtt tctcttacta
     2461 agtttaattt taacagtttt attctctttg ggtcctatcg ccagagcaaa cgatgaaatt
     2521 atcgatcagt ccattgaagc tcttcatgat ctttccaaaa tacctgagcg ggatgtattt
     2581 ttctaccttt tggaaaaagc tgaagctatt gctattttcc ccaaggtagt tcgattgggg
     2641 ctgggattag gagctcaata tggtgatggc ttagtatatc gaaaagagtt caataaaaat
     2701 aactggtatg gtccagcttt ttataaaata tatggagtaa gctttgggcc tcaagtcgga
     2761 attcaatcta ccagcttaat tcttttaatt atgaaccagg atgggatgga aattttttat
     2821 aatgatggtt taaccctggg aggaaacgta agtgttgcag caggaccaat tggaagaagt
     2881 ttttcggccg aagtcgattt gaatttagat tcttcaattt attcttattc acaatctcga
     2941 ggtttatata tcggtctatc ggttcagggg gcacaagtca agcaaaatcg tcaagccaat
     3001 cgtcaacttt ataaagaaag catcgatcca gaggaaattc ttatgtcaaa ggtttcatca
     3061 aaccccgcat ctttgcgtct tctacaaatg ataaaagatt tgattacaaa aaactcggtt
     3121 gatgattatt aaaggagggt aaagcaatga gaacgatttt atccattctt ggtgtgattt
     3181 ttctcattat tttggctttt cgttttttcc cagttattat tgcgggtgtt ttggtggtaa
     3241 tcggtgctat tttgctgatt ctccttttaa ctggtgtggc tataatagcg gcacccattc
     3301 tcatcttact acttctcatt ggggtagtgg tctgggtgct tcgattgatt ttttagaact
     3361 atccttaggg cttgatttat ccatcaagcc ctcccctttt ttatattatt ccaggtggag
     3421 aagcgatgaa tgtaggatat ttttgtagct atatacctga agaaataatt caatcctgta
     3481 acgcaacacc ggtatttcta tcgtccgatt cacttgctac aacccaagct ataccttatc
     3541 ttcccactcc tttttgtcca ttttccaaaa ttcttctcaa ttctctcctc gccgagaaaa
     3601 acctggagtt gaattttatt atttttgggg gaggttgtga tgccggaaga aaactttatg
     3661 atatttttat tgctcttcaa ccaaaaaccc cctgtcatta tcttcatatc ccactggtca
     3721 gtaatcaaga atcattaaaa ttttaccgtc actctctcaa tgatttagcc aataaacttc
     3781 tttccttcca agggatatct caatataatt ttatcgaaaa tcttcggatt atccttgacc
     3841 actctttcac tgtaaaacaa acccgaagcc aaactttctt gactggagaa cttcctggtg
     3901 attctttgct ttgggataaa atccaactcc aaagtagctt ggattctacg atttcttcaa
     3961 aaatttcgat tcttcttctt gcttctcatc tttttgttga aaatatcatc caactattag
     4021 aagaaaagga attccgagtt tttgatggat cagccacggg gatgagacgt tatatcttcc
     4081 ctgatatcga atatactcct gaacatcatg ctttagatac cttagctcga tggtacttgg
     4141 taaacaaagt tccttgtccc gggtataatc ctcaaaaacg cttgggaatc ttacaaaaat
     4201 tcatcaatcg agtcggtata caaggtatag tttattttta ccctaaattt tgcgatcaat
     4261 cactttacga cctatctttt ttaaaaaaac agataaaaat acccctcctt cccgttgaac
     4321 acgatatgtc ctattcttcc ataggacagt gggaaactcg catcgatgct tttcgggaag
     4381 ttttattaag tcaatgaacc taaaagcatc aacagctctt tcacttgccc ggttttttgc
     4441 caataggaag agtcttttcc acttattagc tatatatgaa tggtggttaa aaagaaaatt
     4501 tccagaaccg accttgcctc aaacatcata tcaattaacc attaaaacta ttcgtgagct
     4561 ttatagtcat tcccgaccag tgatttggtc gagcctcttt ttcccctgtg aatttatcca
     4621 tgctgtcggt gccattcctt tttatcccga aatcacaatt ggattagttg ccgctcttgg
     4681 tttctcgaat cttcccttag aatttgccga aaaaaattgg tattctcaag acctctgtag
     4741 ttatcatcga gcaggagtgg gaatgagcct tttcaaacta tttcctcgtc ctgatttttt
     4801 attagcaaca accagtatct gtcggggtac tgctggattt tttcaatcct tagctaacat
     4861 ctggaaaata ccttgctatt taattgatgt tccctaccag atgaccccag aagctattaa
     4921 ttatgttcaa aatcaactct cctcaattgc tacactcatt tcttcccaaa taaattgtca
     4981 acttgattgg gaagaaacct tttcattatc caatgcaact gtcaaaatca ttcatcaaat
     5041 cgaaacatta agaaagcaaa agagttttgc cattcaaccg ccaaccaaaa atttagacta
     5101 tctcccctat tactttcaat ttcttggatc gccagcctca aaattctttt ttgaaaagtt
     5161 tttggaacac ctgcaaaaac tgaaaaatcc gtctaccaat tctcaccggt taatatggct
     5221 tcatctcaaa cccttctatc ccaatcaatt aaccgcactt ctcaatgaat atcactttga
     5281 tgtggtatat gacgagttcg catcagtctt ttgggaacca ttagaaccag aaaaaccttt
     5341 tgaatctctg gctaaaaaaa tcatctccag tcaaaaccta actgttccag aaaaaagaat
     5401 acttagaatt cttaattggt gtgaacaatt taaagctgat ggggttattc aatttaatca
     5461 atggggttgt cgtcaaagcc aaggaatgaa ttttctgctt aaaaataccc ttcaacaaaa
     5521 aggctatccc gtgttattat tagatggaga tcatatcgat aagaagttct attctgaaga
     5581 gcaacttcgt acccggatag aagcatttca tgaaatactg gaaggataat aaccatggta
     5641 tttgctgggc tggatatcgg ttctcaatct acttgcgcaa ccattatcaa tgaaaaggaa
     5701 attatggcaa gtgttcttat gccgagtagt gtgaatccaa aaaaaattgc agaagaagcc
     5761 cttaaccttg ctttaggaaa agccaatctt tcccgatctg atgtccaata tttcattgct
     5821 actggttatg gtcgttatca ggcagaagct aatgagaaga tcagtgaaat cacttgtcaa
     5881 gctcggggtg ttcaatggtt tattcctgaa gcgaggatgg ttcttgatat tggtggacag
     5941 gatagtaaaa taatcctcct cggtaaaaac ggccgtgtta ttgattttat gatgaacgat
     6001 aaatgcgcag cagggactgg ccgtttttta gaattaatga cccaagtttt aggtattcaa
     6061 atagaagatt atggaacctt agtcaaccaa tcaagagaat tcattgaact atctaatacc
     6121 tgtgcaattt tcgccgaatc agaattggta agtttaattg ctcaaggaaa aaagaaagaa
     6181 gatctggctc gtgccgtctg tcgttcagtg gttaaaagga ttttaatcct tgccggaaaa
     6241 atgaacatca ctcggcccct ggtttttact gggggcgtgg ctaaaaacca gggaatcatc
     6301 actatattaa aggaagagct ggctttctcc ccgctcatcc ctcccgatcc tcttattacc
     6361 gctgcccttg gggctgcttg cttagctcga gaaaaagccc ttcccgaacc atagaaaatt
     6421 gttttccaat atttattaca taatatttaa ggctttctct ttggcacgaa catcagaaac
     6481 accgatatcc ctagtatgat cggttttcac ccattggctt tggttccgac ttagaaaagc
     6541 cgcataaaac ataattggtg gccaggttaa tccataaaga aaataactta aaatatctgg
     6601 aatgtagcgc aaagtaaatt taccccaata aaaggatggc cccataacca cattaaaagc
     6661 acattggatt aaacccgaag ccagccagag aggccaaata gatattccaa agttccaaga
     6721 agatccaatt gcaattctaa cggcaaggat agtgaatccc actacagtcg ttacggccga
     6781 aaggaggtaa aataaccaat caagatactg aattttccag gtttttaacc attgcaataa
     6841 taattgggga ccgtatttca tgagcaccca gtaatgacct tgcatccagc gggttcgttg
     6901 ccggcgtgat gcccaaaaag tgaggggttt ttcatcaaag attatagctt cattattcca
     6961 atgaactcgg ctcccactca aaataattcg ggtagaaaac tcaaggtctt cggtgagact
     7021 ttttaaatcc catccaatgg tttttaaaca actgacggta acgaccaatc cagtaccgcc
     7081 taaagcagct gaaagccccc aattggagcg agccaactgc caaaatcgat tggtatacca
     7141 ataacttaaa gcataagatc gactgatcca ggtatcatcg ggatttttag tatcgagata
     7201 cccctggata gcttgggctt taggatgact ttccatataa tcgttcattt ttgctaagaa
     7261 atttttttca acaaggttat cggcatcaaa cattgccact gcatcataat tttccaaagg
     7321 aatttctctt aaagcccatt gaacatttcc ggtcttgcca atatgtaacg tatcatgttt
     7381 ttctaacaca tttacattat agcttttggc aatttcggca gtcttgtctt gacaattatc
     7441 acaagaaaca tagatatcaa agtaattttg gggataattt tgtttaatta aactttccaa
     7501 taaatttcca accacttttt cttcattatg ggcaggaatt aaaacggcaa atcgatgaaa
     7561 acttttagca ggagggagtg gtggtggact ccatgcacca cgtactgcaa taaatatttg
     7621 ataagcccag taaataaaaa gatatacaat aaatatcgca acacccggtc ttatccaagc
     7681 ctcatcccac aaccagtgca ctataacacc tccatgctta cacaccaata gaatagaaaa
     7741 caaaaaaaat ttaataaaat tcctaaaata caattaaatc attatcatct tcccacgatt
     7801 ttgctcctac aatatctgag aaaaataatc caaaaaaaaa gaagaccctt aaggccttct
     7861 ccctatcgta ttatcaacga gctcaatcaa aaaatcatta cctattaagg attttactat
     7921 tttatcaaaa aagtatataa aaaaaatgct ctttttaaac ttttttaaaa tattttactt
     7981 ttgagcccat ttttgttttt ctaaatacat acttgaaaga actgaaaata caataaccaa
     8041 accaaatacg gtgtaggtcc aaaaataggg
//
LOCUS       DAOSOL010000023         7917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_9136_length_7917_cov_4.387688, whole genome shotgun sequence.
ACCESSION   DAOSOL010000023 DAOSOL010000000
VERSION     DAOSOL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7917
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9136_length_7917_cov_4.387688"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PK171_01135"
     CDS             complement(<1..319)
                     /locus_tag="PK171_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT04838.1"
                     /translation="MKYLRFQKPDTNQWSWGIIENGTMIEEFSNSYFNSLGKNGLSHQ
                     LTQVKLGSPTLPTKIVAVGLNYHDHIQEFGHEVPDHPILFIKPSTAVLEPGGTILLPP
                     QSNQ"
     gene            complement(353..1435)
                     /locus_tag="PK171_01140"
     CDS             complement(353..1435)
                     /locus_tag="PK171_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HOT04839.1"
                     /translation="MKHTITLIPGDGTGPEITETVVRVLEATGLKIEWDVQEAGSDVL
                     EKYGTPLPNQVLESIKRNKVALKGPITTPVGTGFRSVNVALRKELDLFACLRPCKTYP
                     GVRSRYENIDLVIIRENTEDLYAGIEFEVNQEATTRLIQNIKELSGHSIRTDTGLSIK
                     PISIFGSKRIVTFAFEYARQNNRRKVTAVHKANIMKYSDGLFLQVARQVASNYPDIEF
                     EDRIVDNMCMQLVQKPELYDVLVLPNLYGDILSDLGAGLVGGLGVAPGANIGTDYAVF
                     EPTHGSAPKYKNQNKVNPMAMILSGMMMLQYIGEKEKAQKLEKAVSKVIQENRFVTYD
                     MKPISNQDQAAKTNEVGETIIRALTK"
     gene            1757..3286
                     /locus_tag="PK171_01145"
     CDS             1757..3286
                     /locus_tag="PK171_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT04840.1"
                     /translation="MNGVEMKGITKTFGSVIANQKINFDLKYGEIHALLGENGAGKST
                     LMNILYGLYHPDSGNIYINGQKCDFRSPFDAIRAGIGMVHQHFMLIPEQTVWENMILG
                     LNNIPQVLPKSEIKRQILELSGKYCLQIDPMSFVWQLSIGEQQRVAILQMLYRKAKLL
                     ILDEPTAVLTPQESQNLFKMLTYMASEGYGIIFISHKLDEVMSLSHRVTILRKGEKVG
                     SVLTGETSKEKLAEMMMGQKFVFSIQKQPKSAGPLVFDVENLRVRGDRGFHVIQDLSL
                     QIHQGEVWGLAGVAGNGQQELCEALSGLRRIESGKIKINQQELTNRPPRHFIKKGLRY
                     IPADRRGVGLIPNMMVDENAILKKYWKNPIAKRSLINWREVARYAHHLIRKYYIAVPH
                     VRYPVKNLSGGNLQKLMLGRELSDSPQVIIAMHPTWGLDVAATKFVREQILKERDRGA
                     SVLLISEDIEELIALSDRLAVICKGEIMGVIDKPQEVTPEVLGLMMAGTPLKNIQKVS
                     A"
     gene            3283..4356
                     /locus_tag="PK171_01150"
     CDS             3283..4356
                     /locus_tag="PK171_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT04841.1"
                     /translation="MSIYFRINKRTSSSWLLSVFLPFISVLLALIGGSIFLSFLKVSP
                     VQAYRGMFQTAFGDWYGISETIVKAIPLILSGIAVTLAFKATVWNIGAAGQIFLGALA
                     TTAAVRYFYVDNRGIMLVIMFLFAGIAGGGWAAIAGYLKARWNVNETITTLMLNYIAI
                     HLADYFIYGPWKDPDSLGFPMTAPFPEAARLPQFAGTRIHYGLFIAIIFAILFWFIFR
                     YTRWGYEIRVIGENPKAAQYAGIYYLRYIVLIMFISGAIAGIAGMGEVSGLQGRLQHG
                     FTAEFGFTAIIVAWLARLNPISVMLVSFLIGALQVGGESLQIVMRLPIASTLVLQGLI
                     LFFVLGGEFFRNYRIRFIRGDER"
     gene            4357..5286
                     /locus_tag="PK171_01155"
     CDS             4357..5286
                     /locus_tag="PK171_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT04842.1"
                     /translation="MEMVISILAAAVRSGTPILFATLGEIIIEKSGILNLGLEGIMLI
                     GALTGFSVSLASGNPWLGIIAAFILGAFITLIHGFISITLKGNQVVSGLAMTIFGTGV
                     SSLIGKAFIGKTIPGLLKTPFPVLNRIPFIGPVFFNHDLMVYFSFFLVVFLSWFLIST
                     RIGLNLRAVGDSPRAADALGLNVNRIRYLAVLIGGGIVALGGAYLSVAYNKMWTESMT
                     AGRGWIAVALVIFAIWNPTRAALGAYLFGGVEAFQLRLQATGTSIPTPLLLMLPYLLT
                     VSVLIFISIGKGKGILFGAPASLGQPYFREESD"
     gene            5357..6445
                     /locus_tag="PK171_01160"
     CDS             5357..6445
                     /locus_tag="PK171_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT04843.1"
                     /translation="MNKWAWLLVLVVFFTLGSAALAFDPIPADHLKIGFVFVGPVGDG
                     GWSYMHDQGRQAIEKAFPGVTTMYAESVPEGPDCARVMEQFVRNGAKLVFATSFGYMD
                     QVIEVAKKYPDVIFMHCSGYKMADNVGLYFGRMYQARYLSGLVAGSMTKSNIIGFPAA
                     HPIPEVIRMINAFTLGARKANPDAKVKVVWLYSWFDPGKEKEATKALIDAGADVIGMH
                     ADSGSTPQTAEESGVYVIGYNNDMSSYAPTKCLTSSVWDWSTTYIDIVKKVVEGTWKP
                     ENSWWGMETGIVKLTSFSQDVPGEVRALVQEEQEKIINGTWDVFWGPIKDQNGNEKVA
                     EGQKMSDQDILSFSWFVEGVEGEIPVSQ"
     gene            6692..7840
                     /locus_tag="PK171_01165"
     CDS             6692..7840
                     /locus_tag="PK171_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOT04844.1"
                     /translation="MEKNNLSTSMFGLSYDQIDCLIEQHDTPLFIIIREKLIDNYQAF
                     RSFFPGVEVYYALKANPHPYIIQVLGEIGSSFDVASLQEIQLVLSHGIPPQRMILANT
                     IKRKKGIRFAKENGVNRLTYDNFDEVGKIARVFQEASVILRVKTPSVGSRINLSYKFG
                     AEPDEALDLLLNAQDAGLNPIGISFHVGSPCTNVENYIASLKLVVKIFEQAAAKGIHL
                     TTIDIGGGFPLQVYQTQNNEVSVESMGKILTPLLLEYFGRECTFVAEPGRSLVGSAGL
                     LISKVIGRAKRSGKNWYYLDDGYYGTFTAIPFDKSVFEFYTLKDDEKKYPCILAGPTC
                     DSVDVIADGVMMPEMQLDDLVIVPNIGAYSWASATTFNGFEKPKVVLA"
BASE COUNT         2258 a         1375 c         1737 g         2547 t
ORIGIN      
        1 cttggtttga ttgagggggg agtagaatgg ttcctcccgg ttcaagaact gcggttgatg
       61 gcttgataaa cagaatagga tgatcgggaa cttcatgacc gaattcttga atgtgatcat
      121 gataatttaa accgacggcc acaattttgg ttgggagagt aggtgaccct aattttactt
      181 gagtgagttg atgagaaagt ccattttttc ctaatgaatt aaaataagaa ttggaaaatt
      241 cttcaatcat tgttccattt tcgataatac cccagctcca ctgattggta tcgggtttct
      301 gaaaccgcaa atatttcaag aaaggtcact ccttttaatc aattttattg aattatttgg
      361 taagagctcg gataatggtt tctccaactt cattggtttt agctgcttgg tcctggtttg
      421 aaattggctt catatcatag gtaacaaagc ggttttcttg gatgaccttg gatacagctt
      481 tttccaattt ctgggctttt tctttttcac caatatactg aagcatcatc attcccgaaa
      541 gaatcatggc cataggattt actttgtttt ggtttttata tttgggagca cttccatgag
      601 ttggttcgaa tacggcataa tcagtaccga tatttgcccc gggagcaact cctaatcccc
      661 caaccaaacc ggcgcctaag tcggatagaa tatcgccata aagattgggg aggactaata
      721 catcataaag ctctggtttt tgaaccaatt gcatacacat attatcaaca atacgatctt
      781 caaattctat atcgggatag ttcgatgcca cttggcgggc aacttgaagg aaaaggccat
      841 cagaatattt cataatgttt gctttgtgga cagcggtaac cttgcgtcga ttattctgac
      901 gggcatattc aaatgcgaaa gtaacaatcc gctttgaacc aaatatcgat attggtttaa
      961 tgcttaaacc ggtatcggtg cgtattgaat gaccacttaa ttcttttatg ttttggatta
     1021 aacgagtcgt tgcttcttgg tttacttcaa actcaatacc agcatagagg tcttcggtat
     1081 tttcccggat gatcactaag tcgatatttt catagcgtga acgaacccct ggataggttt
     1141 tacaaggacg taaacaagca aaaagatcca gttcctttcg aagagcgaca ttaacactac
     1201 gaaagccggt tcccaccggg gttgtaattg gccccttgag ggctactttg tttcttttta
     1261 ttgactcaag tacctgatta ggaagcgggg taccatattt ttcaagaaca tctgatccag
     1321 cttcctggac gtcccattca attttaagac cagttgcttc cagaacacga actaccgttt
     1381 cggtgatctc cggaccagtg ccatctcccg gtatgagagt tatggtgtgc ttcatgatct
     1441 atcgctctcc ttcttaataa ttatttcgat aaaaaaattt tgctcatttt aaaaatattt
     1501 cgaaaggcag ctattaaaga ataacacttg tgcaatatta ataaatataa agcttttttt
     1561 caaccggtaa tatttgatat aattcgctaa tgagtatgtt ttgtttcctc aagaggatta
     1621 gatttagcct gctcagttta aaaaaccagt tttctttgtt tgctaaatat tgtgattttt
     1681 cttttgcttt aattcaattc agagagtaga ataattattt ctaaagtgcc tgttcgttta
     1741 tttggaggat taattgatga atggcgttga aatgaaagga attaccaaga cctttggtag
     1801 tgtaatagcc aatcaaaaaa ttaattttga tttaaaatat ggagaaatac atgctctatt
     1861 aggagaaaat ggcgctggta aatcaacatt aatgaatatc ctatatggtc tttaccaccc
     1921 cgactccggt aacatttata ttaatggtca aaaatgtgat tttcgttccc cctttgatgc
     1981 aatccgtgcc ggtattggga tggttcatca gcactttatg cttattcctg aacaaaccgt
     2041 ctgggagaac atgattttag gtttaaataa tattccacaa gttcttccca aatcagaaat
     2101 aaaaaggcaa atactggaat tatcgggaaa atattgtctt caaatagatc cgatgtcgtt
     2161 tgtttggcaa ttgtcgattg gggagcaaca aagggtagct attttacaga tgctctatcg
     2221 gaaggcaaaa ctacttattc ttgatgagcc aacagcagta ttaacccctc aagaaagcca
     2281 gaatttattt aaaatgctca cttatatggc atcggaagga tatggaataa tttttatttc
     2341 gcacaaatta gatgaagtga tgagtttatc ccatcgggta accattcttc gaaaaggaga
     2401 aaaagtggga tcggttctaa ccggtgaaac ctccaaggaa aaattggcgg agatgatgat
     2461 gggtcagaaa tttgttttct cgattcaaaa gcagccaaag tcagctggac ctttagtttt
     2521 cgatgttgaa aatcttcgag ttcgtggaga tcgtggattt cacgtcattc aagacctttc
     2581 cttacagatc catcaaggag aagtgtgggg gttggctggt gtagcaggaa atggtcaaca
     2641 ggaattatgt gaagcattat caggattacg aaggattgag tcaggaaaaa taaaaataaa
     2701 tcagcaagaa ctcaccaatc gaccgccccg acattttata aaaaaaggac ttcgctatat
     2761 tccagctgac cgacgtggtg ttggtctaat tccgaatatg atggttgatg aaaatgcaat
     2821 attaaagaaa tattggaaaa accctatagc caagagatca ttgataaatt ggcgtgaagt
     2881 tgctcgctat gcccatcatt taattcgtaa atactacatc gctgttcctc atgttcgcta
     2941 tccggtaaaa aatctttccg ggggtaatct gcaaaaattg atgttggggc gagaactcag
     3001 tgattcacct caagttatca tcgctatgca ccctacctgg ggcttagatg ttgcagcgac
     3061 caaatttgtt cgggaacaaa ttctaaaaga aagagaccga ggagcttcag ttcttttaat
     3121 ttcagaagat atagaagaat taatcgcttt aagtgaccga ttagctgtta tatgtaaggg
     3181 agaaatcatg ggggtaattg acaaaccaca ggaagtaacc cctgaggttt taggattaat
     3241 gatggcagga acaccactaa aaaatatcca gaaggtttct gcatgagtat ttattttcga
     3301 attaataaac gaacttcttc ttcctggtta ttaagtgttt ttctaccttt catatcagtg
     3361 ttgttggcct tgattggtgg gtcaattttc ttatcatttc tcaaggtatc cccagttcaa
     3421 gcttatcgtg gaatgtttca gactgcattc ggtgattggt atggaataag tgaaaccatt
     3481 gtaaaagcca ttcccttaat tttatcaggt attgcagtca ctctggcttt taaggctacc
     3541 gtttggaata tcggtgctgc cggccaaatt tttttagggg ctttagcaac gactgcagca
     3601 gttcgatatt tttatgtaga taatcgcggg ataatgttgg ttattatgtt tctatttgct
     3661 ggcattgctg gtgggggttg ggctgctata gcaggatatt taaaagcccg ttggaatgta
     3721 aacgaaacca taactacctt aatgttaaac tatatcgcca tccacctggc tgattatttt
     3781 atttatggac cgtggaaaga tccggatagc ttaggttttc ccatgactgc tcctttccca
     3841 gaagcagctc ggcttccaca atttgccgga accagaatcc attacgggtt atttattgca
     3901 atcatttttg caattttatt ttggtttatt ttccgttata ctcgttgggg ttatgaaata
     3961 cgagttattg gtgaaaatcc aaaagccgct caatatgccg gtatttatta tttaagatat
     4021 attgtcttga tcatgtttat atctggagct atagctggta ttgccggtat gggggaagtt
     4081 tcaggtttac agggacgctt acaacatgga tttactgcag aattcggatt tactgcgatt
     4141 atagtcgctt ggttagctcg cttgaatccc atttcggtta tgttggtttc ttttcttatt
     4201 ggtgccctcc aggtaggagg tgaaagcctg caaattgtaa tgcgattacc cattgctagc
     4261 accttggtgt tacagggatt gatactattt tttgttttag ggggagaatt ttttagaaat
     4321 tatcgaatcc ggtttatcag aggagatgaa cgctaaatgg agatggttat ttccattctg
     4381 gctgcagcag ttcgaagtgg cactcctatt ttatttgcaa ctttgggaga aattatcatt
     4441 gaaaaatcgg ggattttaaa cttaggatta gaaggaataa tgctgattgg ggcactcact
     4501 ggtttttcgg taagtttagc cagtggtaat ccttggctgg gaataatagc agcctttatt
     4561 ttaggagctt ttataaccct catccatggt tttatttcta ttaccttaaa aggcaatcaa
     4621 gtagtgagtg gcttggcaat gacaattttt gggaccggag tcagttccct cataggaaaa
     4681 gcattcattg gtaagacaat tccaggttta ttaaaaaccc cttttccagt cttaaaccgc
     4741 attcctttta ttggtccggt tttttttaat catgacctga tggtgtattt ttcctttttc
     4801 ctggtagttt tcctttcctg gtttttaatc tcgactcgga taggtttaaa tttacgagca
     4861 gtaggtgata gtcccagagc tgctgatgcg ttgggattaa atgtcaatcg gattcgttat
     4921 ttagcagtgt taattggcgg cggtatcgtt gctttgggtg gagcttatct ttcggtagct
     4981 tataataaaa tgtggaccga atcaatgact gcgggaagag gttggattgc agtagcgctg
     5041 gtaatatttg ccatttggaa tccaacccgt gctgctttag gagcttatct ttttggaggg
     5101 gtagaagctt ttcaattaag gcttcaagca acaggtacga gtattccaac gcccttactt
     5161 ctgatgcttc cctatcttct tacggttagt gttctaattt ttatttcaat tgggaaaggc
     5221 aaggggatcc tatttggagc tccagcttct ttgggtcaac cctattttcg tgaagaaagt
     5281 gattaagcga acgaatatca agtataattt ctttaccatt aatttaataa ataaggaaag
     5341 ggagtgtcat aaggaaatga acaaatgggc atggttattg gttttggtgg tttttttcac
     5401 cttgggatcg gctgctctgg cttttgatcc gattcctgcg gatcacctca agattggctt
     5461 tgtctttgtg ggtccggttg gtgatggtgg atggagctat atgcacgatc aaggacgtca
     5521 agctattgaa aaagctttcc ctggggtaac gacgatgtat gctgaatcgg ttcctgaggg
     5581 gcccgattgt gcgcgagtta tggagcaatt tgttcgaaat ggagcgaagt tggtttttgc
     5641 tacttcattt gggtatatgg accaagtcat tgaagtggcc aaaaaatatc cggatgttat
     5701 attcatgcat tgttcgggat ataaaatggc cgacaatgtc ggtctctatt ttggaagaat
     5761 gtatcaagcg agatatctat cggggttggt ggcgggaagt atgacaaaaa gcaatattat
     5821 cggttttccg gcagctcatc cgattcctga agtaatccgt atgattaatg cttttacctt
     5881 aggagctcgt aaagcgaatc ccgatgcgaa ggttaaggtt gtttggcttt attcttggtt
     5941 tgatccagga aaagaaaaag aagctacgaa agcacttata gatgcgggag ctgacgtgat
     6001 tggtatgcat gccgattctg gatcaactcc tcagactgct gaagaatcag gggtatatgt
     6061 tattggttat aataatgata tgtccagcta tgctccgacg aaatgtctta cctcctcagt
     6121 ttgggactgg tcaacaacct atattgatat tgtaaaaaag gttgtagaag gaacctggaa
     6181 acccgagaat agttggtggg ggatggaaac tggtatagta aaactcacgt cctttagcca
     6241 agatgtacca ggtgaagtga gagccttggt tcaagaagaa caggaaaaaa taatcaatgg
     6301 aacctgggat gttttttggg gtccaataaa agaccaaaat ggaaatgaga aagtagctga
     6361 aggacagaaa atgtccgatc aagatatctt atccttctcg tggtttgtcg aaggagtaga
     6421 aggagaaatt ccggtaagcc agtaataacg ctgaaaatta aagggcactt taatcaacca
     6481 tcgggccagc atcttaaacc aaaaaatttt gttggctcga tggctttaaa ctattgaaaa
     6541 tccgataatg aaaggttata cttcacaatt gtcagaataa ggacttctta agcattgatt
     6601 caaatttttt cctcctaagg atgatcgttc ttttttgctc caattttgat atttatgatt
     6661 tccatatttt aaaagcggga gttgattatt tttggagaaa aataaccttt caaccagtat
     6721 gttcggttta agctatgatc aaattgattg tttaattgaa cagcatgata ctccactttt
     6781 tatcataatt cgagagaagt taatagataa ttatcaggct tttcggtctt ttttcccagg
     6841 ggttgaggtt tattatgcct taaaagccaa tcctcatcct tatattatcc aagttttagg
     6901 tgagatcggc tcgtcatttg acgtagcatc ccttcaagaa attcaactgg ttttaagcca
     6961 cggtattcct ccacagcgca tgatattagc aaatacgatt aaaagaaaaa aaggtattcg
     7021 ttttgccaaa gaaaacggtg ttaaccgctt aacttacgac aattttgatg aagtgggtaa
     7081 aatagcccgg gtttttcaag aggcatcggt tatcttaagg gttaagacgc cgtcagttgg
     7141 cagccggatc aatctttcct ataagtttgg cgctgaaccc gatgaagcct tggatttgtt
     7201 gctcaatgcg caagatgctg ggttaaatcc aattggtatc agctttcatg tcggttcacc
     7261 ctgcacgaat gtggaaaatt atattgcttc tcttaaattg gtggtaaaaa ttttcgaaca
     7321 agcagcagcc aaggggatac accttactac tattgatatc ggaggtggtt tcccactaca
     7381 ggtttaccag actcaaaata atgaggtgag tgttgaatcc atgggtaaaa tactcactcc
     7441 cttattgttg gaatattttg gcagagaatg tacttttgtt gctgaaccag gaaggagttt
     7501 agtgggaagc gctggattat tgatttcaaa agtaattgga agagctaaac gctcgggtaa
     7561 aaattggtat tatttagacg atggatatta tgggacattt acagctattc cttttgataa
     7621 atcggttttt gaattttata cgttgaaaga tgatgagaaa aaatatccct gtatcttagc
     7681 aggaccaact tgcgattctg tcgatgttat tgccgatgga gtcatgatgc ccgagatgca
     7741 gctggatgat ttggtgatcg ttcccaacat tggtgcctat tcatgggcct cagcgacaac
     7801 atttaatggt tttgaaaagc ctaaagtcgt tttagcctga aaaatccacg gacttgataa
     7861 atcaagctcc tacaaaaaat ataaatttgt aagggcgtaa tttattgcgc ccgatta
//
LOCUS       DAOSOL010000024         7891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_9176_length_7891_cov_4.289689, whole genome shotgun sequence.
ACCESSION   DAOSOL010000024 DAOSOL010000000
VERSION     DAOSOL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9176_length_7891_cov_4.289689"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK171_01170"
     CDS             complement(<1..237)
                     /locus_tag="PK171_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT04845.1"
                     /translation="MLRELVIKDFVIVDSQHLELEGGLVAITGETGTGKSLIIDAISL
                     LKGGRAVEGMIRRDRKSALIEGLFDLSFSPDLVHW"
     gene            complement(240..1094)
                     /locus_tag="PK171_01175"
     CDS             complement(240..1094)
                     /locus_tag="PK171_01175"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOT04846.1"
                     /translation="MNRNPIQRIHIFSRKVNDMISQKAQELREFFNQYQVSCTIIDTN
                     QIPKEKPDMVFVLGGDGTFLSANHSYAAEGIPILGIDMGGLGFLTEVSVEALFNAAKA
                     VLEGNYTVENRMMVDCSIHHTNKEVEQDIALNDVVIYRGPFAQMIRLSTFIDDEYLAT
                     FPADGLIIGTPTGSTAYSLSAGGPVIHPTLDLFIITPICAHTLYARSIIVQPNSSIRL
                     ILESTKEGTMVTLDGQRGFTLDKGDYIEVRKSKLTNHMVKLIPEKPFYSLLRDKLSWG
                     MDIRKRID"
     gene            complement(1129..1956)
                     /locus_tag="PK171_01180"
     CDS             complement(1129..1956)
                     /locus_tag="PK171_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOT04847.1"
                     /translation="MMEEKKQKIRIDELLVKRGLVESREKAQRMILAGDVKVENVSGI
                     LKPSSRVWDDTKVALERLPQYVSRGGEKLAKALSYFQIDPAGKVFLDIGSSTGGFTDC
                     LLQKQAQKVFALDVGYGLLHEKLRKNPQVIPLERINIRYYVPQDLPEPIQGITIDVSF
                     ISLRLVFPVISSLLPKGGICIALIKPQFEAGRDKVERGGLVRKKETHIQVLKDVLESA
                     RDNHLQLQGITFSPIQGGGGNIEYLAYWMKCANFFDKTEFGDIIRKEVQEAHHFFTK"
     gene            complement(1988..2878)
                     /locus_tag="PK171_01185"
     CDS             complement(1988..2878)
                     /locus_tag="PK171_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT04848.1"
                     /translation="MDIIQYINENAILLDNHLEQRLFFPEAPTRLMEALRYGVIGGGK
                     KLRASLCLATGEAYGIKREDLLSLAGGIEMIHSFSLVHDDLPEMDNDDYRRGKYSLHK
                     AFGGAMGILAGDALLVEGFRFILSDSHFCRLVGNTKLLKILNVLLDALSVEGMVGGQV
                     LDIDLEGTEADEEQVMDIYRMKTARFIQAPILCGAIVGGVNKKELNDLERFGLLAGEC
                     FQIKDDLLDVTQPSEILGKTAGKDVQQEKATLVKIYGIHGTQEIMENLFREAEAILNQ
                     TNRSFSLLREIAHFIITRHH"
     gene            complement(2878..3312)
                     /gene="nusB"
                     /locus_tag="PK171_01190"
     CDS             complement(2878..3312)
                     /gene="nusB"
                     /locus_tag="PK171_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT04849.1"
                     /translation="MRRKVRETALQVLFQMDLRKKPLSEILSVFKIPLNWKDDDRSFF
                     LSLVRGVEQNLPEIERIISAYSLEWPLYRMPTIDRNLLRMAVFEMFYLSDISVGVSIN
                     EAVELAKKYSTNDSGKFVNGILGKLARSSETEFKSLGVNKKE"
     gene            complement(3302..3688)
                     /locus_tag="PK171_01195"
     CDS             complement(3302..3688)
                     /locus_tag="PK171_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOT04850.1"
                     /translation="MKYVDIHLSEGKIEYSHRVLLKLIEDIVLQVSGIDSLEKQPDES
                     IKIETKKESLNISLYLNFTLEKRVPEVAWDVQKSLKDNFEKKTGLKVDRIDIFVQKFS
                     SVGLNENFENLFSESSAPELKVNYAP"
     gene            complement(3700..4266)
                     /gene="efp"
                     /locus_tag="PK171_01200"
     CDS             complement(3700..4266)
                     /gene="efp"
                     /locus_tag="PK171_01200"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOT04851.1"
                     /translation="MADIISNTDLRVGTCIDVDGTLYIVTAFQPAKFGKWSWVTKTKL
                     RDINSGFIVEKVFKPGDKIVRAILEGRQAQYMYKDDGGFHFLDQETYEEVLLPDDLVG
                     EASDFLVENMMVEILMYGDKHVTINLPSYVELKVVDAPPGIKGDTASGGSKPATLETG
                     IVVQVPLFVNIGEVIRVDTRTREYLERA"
     gene            complement(4334..5395)
                     /locus_tag="PK171_01205"
     CDS             complement(4334..5395)
                     /locus_tag="PK171_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOT04852.1"
                     /translation="METNRIKKLQENMKNNRDIPFLLTDLSNIFYLTGFTGSSGFLIV
                     FPDRPPVFLCDGRYTTQAKKEFITSTEIVEFNTDVYKRIADILTSSGFQTVYFETSLS
                     YQSYLNLKEKNLELVPVPNWLEEMRMIKSPRELELIERALHLSEKAWEAVSPMIRPGI
                     KEKDFALELDYQMIKAGGDSIAFSTIVASGERSALPHAQPTDERMEAGSWLVVDWGVR
                     YQKYCGDITRTVPVGCVEDLWFEETIQLLKEAQQMAQEFIRDGVRAADVERTVRNFLN
                     QHKIEQYFTHSLGHGIGIQVHESPRLSNNSEVILRENMVVTVEPGVYIPGKGGIRLEN
                     IVVVEKESCRILNRLPVIL"
     gene            complement(5415..5873)
                     /gene="aroQ"
                     /locus_tag="PK171_01210"
     CDS             complement(5415..5873)
                     /gene="aroQ"
                     /locus_tag="PK171_01210"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOT04853.1"
                     /translation="MNQRLLFIFGPNLNLLGERETSLYGSMSYDTLIMKIAEEAKKRG
                     VQTEFFQSNHEGEIVDKIHQKRKEIDGIIINPGALTHYSIAVRDALSAVKIRSIEVHQ
                     SNIYAREEFRHHSVTAPVCWGQISGLGWIGYILAMDALIMKGKEEAIRLA"
     gene            complement(5870..6967)
                     /gene="aroB"
                     /locus_tag="PK171_01215"
     CDS             complement(5870..6967)
                     /gene="aroB"
                     /locus_tag="PK171_01215"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOT04854.1"
                     /translation="MRVLSIQTKTKHYPVILLPSLFATSDWLNQVQFPSRKVTIVTNS
                     TVGPLYAQKIIKILKEKDFIPSMVTIPDGEEYKQLNTVEKIYHELLNQGLDRKGFLIA
                     LGGGVITDITGFVASTYLRGIPYFQIPTSLLAQVDSSVGGKTGVNVEEGKNLIGTFYQ
                     PDGVIIGLDFLQSLPDREFREGLSEVIKASLLKGGEFFEFFRKNISMILGQDQKTLEE
                     VIYQSIQFKGEIVQEDEKESGVRSILNYGHTLGHAFEKTLGYGALRHGEAVAVGMIGA
                     AMVAEKMGMISTDLVNIHQKLLVRCGLPTSFPYSVKADSFIQSLLKDKKNVSGSLRMV
                     LLKAIGKPVYSTPVEMGLVLDILPQLVEVEP"
     gene            complement(6936..7454)
                     /locus_tag="PK171_01220"
     CDS             complement(6936..7454)
                     /locus_tag="PK171_01220"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT04855.1"
                     /translation="MNIALIGFMGSGKTTIGKKLARQLGWTFLDSDGLIQKKENCSIP
                     EIFAQKGEVYFRECEKSVLTEMAELKKVVLATGGGLPIGEDNWKILRQSFFTVYLKAH
                     FDELFLRITGDPIRPLLQKYPTKNQLKELYLSRVDWYEKAHVIIDTSQKKLERIIGEI
                     IERARPFYTNQN"
     gene            complement(7458..>7891)
                     /locus_tag="PK171_01225"
     CDS             complement(7458..>7891)
                     /locus_tag="PK171_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04856.1"
                     /translation="PINNVGAEIQDCEVVYTDNNGIAINNLKKSLSVYLYIPPFSLNV
                     ENNEETDEGTMTGLSRTLKLEIFDQSTQDYYINHNIPFGQAIITFNGLDGAGHPIALK
                     KAISINADMIDDSLQDEIDQQIQEIIQNQGQNPGSSCSSCS"
BASE COUNT         2347 a         1755 c         1326 g         2463 t
ORIGIN      
        1 ccagtgaact aaatctggac taaaggatag atcaaaaagt ccttcgatga gggctgattt
       61 tcggtcacga cgaatcattc cttcaaccgc tcgtcctcct ttaaggagac ttatagcatc
      121 aattatcaaa gatttaccgg ttcccgtttc accggtaatc gctaccagac caccttctaa
      181 ttcaaggtgt tgagaatcaa caatgacgaa gtctttaatg accagttctc ggagcattcc
      241 taatcaatcc gttttctaat atccatcccc caggacaatt tgtctctcaa caatgaataa
      301 aagggtttct cgggaataag ctttaccata tgattggtaa gtttcgattt tcgaacttca
      361 atataatctc ctttatccag ggtaaaaccc ctctgtccgt ccaaagtaac catagttcct
      421 tccttggttg attctaatat cagccgtatc gaggagttcg gttgaacgat aattgatcga
      481 gcgtaaaggg tatgggcaca gataggagtt atgataaaaa gatctaaagt tggatgaata
      541 accggaccac ctgctgataa agaataagca gttgaaccgg taggagtgcc aatgatcaac
      601 ccatcggcag gaaaagttgc cagatattcg tcatcgatga aggtgctgag gcgaatcatc
      661 tgagcaaagg gacctcggta tatcaccaca tcatttaaag caatatcttg ctccacttcc
      721 ttattggtat gatgaataga acagtccacc atcattcggt tttcaacggt gtaatttcct
      781 tctaaaaccg cttttgcagc attgaacaag gcctctacac tcacctcggt caaaaagccc
      841 aatccgccca tatcgatacc caatatcgga attccttcag ccgcataact gtgatttgcc
      901 gataaaaaag taccatcgcc acctaacaca aaaaccatgt ctggtttttc tttaggaatt
      961 tgattggtgt cgattatagt acatgatacc tggtattgat taaaaaattc acgcaattct
     1021 tgcgcttttt gggaaatcat atcattcact tttctggaaa aaatatgaat tcgctgaatt
     1081 ggatttctat tcatcctatc tcacttttta taattatttt cggctgattc attttgtaaa
     1141 aaaatgatgc gcttcctgta cttcttttct tattatatcc ccaaattcag ttttatcaaa
     1201 aaaattagcg catttcatcc aataggctaa atattcaata ttccctcccc ccccttgaat
     1261 tggggagaaa gtaattcctt gaagttggag atggttgtct cgggctgatt caagcacatc
     1321 cttaaggact tggatatggg tttctttttt cctcaccaga ccaccacgct ccactttatc
     1381 tctcccagct tcaaactggg gttttattaa agcaatacat attccccctt ttggaaggag
     1441 ggaactgata accggaaaaa ctaggcgaag tgagataaag gacacatcaa tagtaatacc
     1501 ttggataggc tcagggaggt cttgtggaac ataatagcgt atgttaattc tctctaaagg
     1561 aattacttgg ggatttttac gaagcttttc gtggagaagt ccatagccga catcaagggc
     1621 aaataccttt tgagcttgtt tttgcaacaa acaatcggta aatcctccgg tcgaagaacc
     1681 aatatctaaa aaaactttac cagccggatc gatttggaaa taagagaggg ccttggctaa
     1741 cttctcaccg cctcggctaa catattgagg caacctttca agagcaactt tagtatcatc
     1801 ccatactcga gatgaaggct ttaaaattcc tgaaacattt tcaactttaa catcacccgc
     1861 caaaatcatc cgctgggctt tttctcggct ttcaactaat cctcttttaa ctaataattc
     1921 atcaattcgg attttttgtt ttttttcttc catcatttta gctatggata gataattcca
     1981 attggtttta gtggtgacgg gttatgataa agtgggctat ctctcttaac aaggaaaagg
     2041 aacgattggt ttggtttaag attgcttcag cttctcgaaa taagttttcc atgatttcct
     2101 gagtcccatg aattccataa attttcacca gagttgcttt ctcttgctgg acatctttac
     2161 cagctgtttt acccaatatc tcgctcggtt gggtgacatc taaaagatca tctttgattt
     2221 gaaagcattc tcccgccaac agtccaaatc gctccaaatc attgagttct tttttattca
     2281 cacccccaac tattgctccg caaagaattg gtgcttgaat aaatcgggca gttttcatcc
     2341 gataaatatc cattacttgt tcctcgtcag cttcagtccc ttctagatca atatctagca
     2401 cttgaccacc aaccatacct tctacgctga gggcatccaa taaaacattg agaattttta
     2461 acaatttagt attaccaact aatcggcaaa aatgactatc ggaaagaata aaacgaaatc
     2521 cttccaccaa caaagcatcg ccagcaagga ttcccatagc tcccccaaaa gctttatgca
     2581 aactatattt cccacgacga taatcatcat tgtccatttc tggtaaatca tcatgaacca
     2641 aggaaaaaga atgaatcatt tcgattccac ccgccaaaga aaggaggtct tcacgcttaa
     2701 taccataagc ctcaccggtt gccaggcaca aagatgctcg gagctttttt cctcctccaa
     2761 taactccata ccgaagagct tccattaatc gggttggagc ctccgggaaa aaaagtcttt
     2821 gttccaaatg attgtctaaa agaatggcgt tttcgttgat atattgtatg atatccatta
     2881 ctctttttta ttaaccccca aagatttaaa ctcggtttca gaacttcgag ccaatttccc
     2941 taaaatacca ttgacgaatt tgcccgaatc gttggtacta tattttttag ccaattctac
     3001 tgcttcgttg atcgatactc caaccgatat atcagataaa taaaacattt caaaaactgc
     3061 catccttaat aggtttcgat caatagttgg catcctatat aaaggccatt ccaaactata
     3121 agcagaaatg atacgttcaa tttcaggcaa gttttgttct actccccgaa ccagtgaaag
     3181 gaaaaagctc cgatcatcat ccttccaatt caagggtatt ttaaataccg ataggatttc
     3241 agaaagtggc tttttcctga gatccatttg aaataaaacc tgcagagcgg tttctctcac
     3301 tttacggcgc ataattgacc ttcagctccg gtgccgatga ttcagaaaat aaattctcaa
     3361 aattttcatt cagtccaacg gatgaaaatt tttggacaaa aatatcaatt cgatctacct
     3421 ttagtccggt ttttttctca aagttatctt tgaggctttt ttggacatcc caagctactt
     3481 ctggtaccct cttttctaaa gtaaaattga gataaagaga aatatttaaa gattcttttt
     3541 tggtttcgat cttgatgctt tcatcgggtt gtttttctaa agaatcaata cctgatactt
     3601 gaagaacgat gtcctcgatc aatttcaata aaacccgatg agaatattca atttttcctt
     3661 ctgacaaatg gatatcaaca tacttcaata tcggccctcc taagctctct ctaagtattc
     3721 ccgagtacga gtatctactc gaatgacctc cccaatgttc acaaacaaag ggacttgaac
     3781 aacgatgccg gtttcaagtg tagctggttt tgaaccaccc gaggcagtat cgcctttgat
     3841 tcctggagga gcatcaacta cttttaattc gacataggag ggaagattta tagttacatg
     3901 tttatctcca tacatcaaga tttcaaccat catattttct accagaaaat cactagcttc
     3961 gccaaccaaa tcgtcaggca gcagaacttc ttcgtaggtt tcttgatcga gaaaatggaa
     4021 acctccatca tctttataca tatactgagc ttgtctccct tcgaggatgg ctctaacgat
     4081 tttgtctccc ggtttaaaaa ctttctcgac gataaaaccg ctatttatat ctcgaagttt
     4141 ggttttggtc acccaactcc attttccaaa ttttgctggt tgaaaagcgg taacaatata
     4201 taatgtacca tcaacatcga tacaggtccc aactcgaaga tcagtattgg atataatatc
     4261 agccactata aaacctccca tgaatcaaaa aaagtcttaa atagcccttt tcatcaattt
     4321 cttaaattta cctttacaaa attaccggaa gcctatttaa aattcgacag gattcttttt
     4381 caaccacaac tatattttcc agtcttatcc cacctttccc agggatataa actcccggtt
     4441 ccaccgttac aaccatgttt tctcgaagaa taacttcact attattactt aatcgaggtg
     4501 attcatgaac ttgaattcca attccatgac ccaaactgtg agtaaaatat tgctcaattt
     4561 tatgttgatt gagaaaatta cgaactgtcc gttcaacgtc agcagcacgc acaccatccc
     4621 gaataaattc ctgagccatt tgctgtgctt ccttcaataa ttggatggtt tcctcaaacc
     4681 agaggtcctc aacacagccg acaggaaccg tgcgggtaat gtcaccacaa tacttttgat
     4741 aacgaactcc ccaatcgact accaaccagc ttcctgcttc cattctctcg tcggttggtt
     4801 gagcatgagg aagagctgac ctttctccgc tcgccactat agttgaaaaa gcaattgaat
     4861 ctccgccagc tttgatcatt tgataatcca attctaaagc aaaatctttt tctttgattc
     4921 cagggcgaat cattgggctc actgcttccc aggctttttc agaaaggtgc agcgcccttt
     4981 caatcaattc aagttctctg ggtgatttta tcatccgcat ttcttctaac caattgggaa
     5041 ccggaaccag ttcaaggttt ttttctttta aattgagata actttgatag ctaagactcg
     5101 tctcaaaata aaccgtttga aaaccacttg atgtgaggat atcggcaatt cttttataga
     5161 catcggtatt aaattcgacg atttctgtgg aagttataaa ctccttcttt gcctgagttg
     5221 tatagcggcc atcacagaga aaaaccggag gtcggtcggg aaatactatc aaaaatccac
     5281 ttgatccggt aaaaccggtc aggtaaaaaa tgtttgatag atcagtcaac aaaaaaggaa
     5341 tatcacgatt atttttcata ttctcttgga gtttttttat tcgattggtt tccatatttt
     5401 tccgatcctt atcattaagc cagacgtatc gcttcttcct ttcctttcat gatgagagca
     5461 tccatagcta agatataacc aatccaaccc aaaccactaa tttgacccca acataccggt
     5521 gccgttactg aatggtgacg aaattcttcc ctggcataga tgtttgactg gtgtacctca
     5581 attgatcgaa tttttacagc actaagagca tctcgaacag ctatactata atgtgttaac
     5641 gctcccggat tgataattat accatcaatt tcttttcttt tctggtgtat tttatcaacg
     5701 atctcgcctt cgtggttgga ctgaaaaaat tctgtttgta cccctctttt tttagcttct
     5761 tcggcaattt tcatgattaa agtatcgtaa ctcattgaac cgtataagct ggtttctcgc
     5821 tcacctaaaa gattcagatt tggaccaaaa ataaagagca atctttgatt catggttcca
     5881 cctcaactaa ctgtggcaaa atatcaagaa caagtcccat ttccaccgga gtcgagtaca
     5941 ctggtttgcc aatggctttt aataggacca tcctcaagct tccactcaca ttttttttat
     6001 cttttaacaa ggattgaata aagctatcag ctttgactga ataaggaaat gatgtaggca
     6061 acccacatct caccagcaac ttttgatgaa tatttaccaa atcagtagat atcatgccca
     6121 ttttctcagc gaccatagct gctcctatca tcccaactgc tacagcttca ccatgacgaa
     6181 gagctccata cccaagggtt ttttcaaaag catgccctaa agtatggcca taatttaaga
     6241 tactccttac tccactttct ttttcgtcct cttgaacgat ttctccctta aattgaatcg
     6301 attgataaat aacctcttcc agagtttttt gatcttgtcc aagaatcatc gatatattct
     6361 ttcgaaaaaa ttcaaaaaat tctcctccct taagcaaaga tgctttaatc acttcactca
     6421 agccttctcg gaattcgcgg tcgggtaaag attgtaaaaa atcaagccct ataataactc
     6481 cgtcgggttg ataaaaagta ccgattaaat tttttccctc ttcgacgttt actccggttt
     6541 tcccaccaac cgagctatca acctgggcta aaagactagt gggaatttgg aaataaggta
     6601 taccacgtaa ataggttgag gcgacaaaac cggtgatatc ggtaatcact ccgccgccca
     6661 aagcaattaa aaatcccttt ctatctaaac cctggtttaa taactcgtgg tagatcttct
     6721 caacagtatt cagctgttta tactcctcac cgtccggtat ggtcaccatt gaaggaataa
     6781 aatccttttc tttcagtatc tttataattt tttgtgcata gagcggcccc acggtagaat
     6841 tggttactat ggtcaccttt cgggatggaa attggacctg attcaaccag tcagatgtgg
     6901 caaaaagtga cggaaggaga ataaccgggt aatgcttagt tttggtttgt atagaaagga
     6961 cgcgcacgtt caataatttc tccaataatt ctttccagct ttttttgaga cgtatcaatt
     7021 ataacatgag ctttttcata ccaatccact cgactcaaat aaagctcttt gagttgattt
     7081 ttggtagggt atttttgtaa caatgggcgg attggatccc cagttatcct caaaaaaagt
     7141 tcatcaaaat gagcttttaa ataaacggta aaaaaacttt ggcgaaggat cttccaatta
     7201 tcttcaccaa tgggaagccc cccaccagtt gctaacacaa ccttctttaa ctcggccatt
     7261 tcggttaaaa ccgacttctc gcattctcga aaatacactt cgcccttttg tgcaaaaatc
     7321 tctggtatag agcaattttc ctttttctga attaacccat cgctatcaag aaaagtccac
     7381 cctaactggc gagctaattt tttaccaatg gtcgtcttcc ctgatcccat aaaaccaata
     7441 agtgcaatat tcatttatta agaacaagaa gaacaggatg agcccggatt ttgtccttga
     7501 ttttgaatga tttcctggat ctgttgatca atttcatcct gcaagctgtc atcaatcata
     7561 tcggcgttga tagaaatagc tttttttaaa gcgatagggt gaccagcacc atctaatccg
     7621 ttgaaggtaa tgattgcctg accaaaggga atattgtggt ttatataata gtcttgagtc
     7681 gattggtcaa aaatctcgag cttcagggtt cggcttaatc cagtcatagt tccttcatca
     7741 gtctcttcat tattttcaac atttaagcta aaaggaggaa tataaagata aacggataaa
     7801 ctctttttta aattattaat agcaattcca ttgttatcag tataaacgac ttcacaatcc
     7861 tgaatttctg ctcccacatt attaatcgga g
//
LOCUS       DAOSOL010000025         7846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_9236_length_7846_cov_3.306636, whole genome shotgun sequence.
ACCESSION   DAOSOL010000025 DAOSOL010000000
VERSION     DAOSOL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9236_length_7846_cov_3.306636"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /gene="pckA"
                     /locus_tag="PK171_01230"
     CDS             <1..1546
                     /gene="pckA"
                     /locus_tag="PK171_01230"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOT04857.1"
                     /translation="LKAHYSFEKSGVFNTKQTFYHLSIPELFEHALQKKEGILSSTGA
                     FSVLTGAYTGRSPEDRFFVDDETTHDLIDWGKANKPISQEIYEHLNAKLVSYLQQKDV
                     YIDDAYVGTDPENRLAVRFINEYAYSSIFVNNMFLEPTAQELQSFIPDFTVLCFPRFK
                     AIPEIDEVRSEAFVIINFKEKKVIIGGTSYGGEIKKAIFTVMNYLLPQKGILPMHCSA
                     NIGKEGDVAIFFGLSGTGKTSLSADIERRLIGDDEHGWSDQGIFNFEGGCYAKCINLS
                     KDKEPQIWDAIRYGAIMENIYLEPDSRQPDYTDDRYTENTRVSYPIEFIPNALIPGIA
                     KHPQVVIFLTADAFGVMPPVAKLTTEKAVYNFILGYTSKLAGTERGIVEPQTTFSVCF
                     GAPFMPLNPKTYAQMLGKKISQHGSRVYLINTGWIGGPYGIGKRIDIDYTRKMVRAAI
                     DGTIDQNGYREDPVFRLLIPKELPDIPNDILNPGNLWNNHSNYTNEAQKLRNRFDEVM
                     KKYLPL"
     gene            complement(1593..2021)
                     /locus_tag="PK171_01235"
     CDS             complement(1593..2021)
                     /locus_tag="PK171_01235"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT04858.1"
                     /translation="MAHQVSIFAENKPGRIEKLTRLFTEASINIRAITISSANGFGVI
                     KVLVDQPHKAFEILRSQGIPSYLQEVIAVIMEDIPGGLHRVAQVLSENSINIEDAYGF
                     VVASGKKAILIIQVESQPRAQSVLERNNFQLLTDEEIYRY"
     gene            complement(2050..3321)
                     /locus_tag="PK171_01240"
     CDS             complement(2050..3321)
                     /locus_tag="PK171_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOT04859.1"
                     /translation="MDRDQLVQLQSKRLQETMQRAAKTSYYKKLFTQNHIQPEKILTT
                     KDLQNIPPTSKEDLRSGFPYGFLAKPLEEVVRLHSSSGTTGVPTVIYHTAADIDSWSN
                     LVARSLYGVGVRRNDIFQNMMSYGLFTGGLGMHYGAEKIGTLVIPIAAGNSKRQIWFL
                     KNFQTTVTHIIPSYALLLSNLIKSEGLDSSRDLNLKIMLIGAEPHSEETRHRIEELYQ
                     AKAFNSYGLSEMNGPGVAFECAYQTGLHVWEDAYFLEIVNPKTLEPCPAGELGEMVLT
                     TLCRQATPILRYRTKDLAYWIDEPCPCGRTHYRISRIQGRTDDMFIYHGVNIFPIQID
                     KVLMNNPEVGSNYLITLERKDLRDTMKIEIEIKPELFIGDLPKLERLRERIQAEIRSE
                     TLVTPEIVLVEPGTLPTTEGKAVRVIDKRQL"
     gene            3577..3792
                     /locus_tag="PK171_01245"
     CDS             3577..3792
                     /locus_tag="PK171_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOT04860.1"
                     /translation="MDIQINGLVSFLAGLLSFLSPCVLGIAPAYVSYISGLNHLKKNE
                     RKFFSTPFFLWLDFLWFLYSWASELLF"
     gene            3738..4298
                     /locus_tag="PK171_01250"
     CDS             3738..4298
                     /locus_tag="PK171_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOT04861.1"
                     /translation="MVGFSMVFIFMGVGASFLGTFLMKYKVWFNRIAGFIIIIFGLQI
                     LGIIKIKMFYAEKRIQPKKTWAKMRSLILGVTFGLGWTPCVGPILGSILLYVSTLGNV
                     LQGGIYLSFYALGLAIPFILLGLGWGYMMSVLRTLQKRGHIIEIISGILLIFLGIILM
                     FDQMNAFIAFLGLENIVPGESWFLNQ"
     gene            4320..5126
                     /locus_tag="PK171_01255"
     CDS             4320..5126
                     /locus_tag="PK171_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HOT04862.1"
                     /translation="MARKSQPVKGFGVVSKKLSLSNRAYRGIHAIKVKNIVGSVGRVQ
                     DLLSGFRLKNPDVRYYRLRKIMEKGDVVPPIIVYKVGNEYYVLDGNHRVAIAKELGIE
                     FIDAEVIEFFPSERKESRTLRKKRIEFENLTGLKGIDLTEHRHYDTFFNYINDYAHQM
                     ELFLKRKVDIKEAALNWFLSVYTPAVQCIVEKGLEDVYQGKTLGDIFCFILDYKWYKS
                     QKEGYDIGFDNAMQGFIEEILGKEEPEEKKTLIEKFEGLLEEVLPWIKKE"
     gene            5108..7741
                     /locus_tag="PK171_01260"
     CDS             5108..7741
                     /locus_tag="PK171_01260"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HOT04863.1"
                     /translation="MDQKGIIWHALSSQEVIQQLETDLEKGIKGEEAKQRLADKGRNV
                     LPTGKRKTILGMFIDQFKDFLILILVAAAAISGLLGETTDATVIIAILIINAVLGVSQ
                     ERKASKALDALKKMSVPECEVIRDGLLQKISSEELVPGDVILLHEGDYVPADLRLIEV
                     YNLRIDEASLTGESVPVEKITDSMAENLPISDRLNMAYSGTIVAYGRAKGVVTETGIN
                     REIGKIAQLLEKEEEVVTPLQKRLAGLGKLLGYLTLIVCGVVFVVGIIRGEQIFDMFM
                     TAVSLAVAAIPEGLPAIVTIVLALGVTRMSQHNAIVRKLPAVETLGSATVICTDKTGT
                     LTQNQMTVQEVFQMNEEGEEVQSRIFMPSEALIQIGVLCNDSSIANNNGKVQRFGDPT
                     ELALIELAEKNGYSVKKIRQDFPRLDEVPFDSKRKMMSTLHELNGKRKILVKGAPDIL
                     ITRCNSYQSGGQILPLGEKDRLFILNTIEEMAGKALRVLAFADKEIPDQKKISQEDEN
                     DLNFQGLVGMIDPPRPEVKQALEEANSAGIETIMITGDNLLTARTIAQDLGLVQSGDE
                     AITGQEMEKLSQETLMEKIKHLRVFARVWPEQKLNIVEALQKNQEVVAMTGDGVNDAP
                     ALKKADIGVAMGITGTDVAKEVADVVLMDDNFATIVKAIEEGRVIFDNIRKFVMYLLA
                     CNIGEIFAIFIPILIGLHRPLVPVQILLINLVTDGLPAMALGVDSPEPDIMMRRPRNP
                     KEGILNPQSMKIILFNSVFIAISVIIAFVAGTRIGGIETGRTMAFVTLAFSELLRAYS
                     FRSEKRNFWQINLRSNLYLIEAALLSAAIVLITVIVPSAAQVFSNVPLNGLEWMYTIV
                     FSFISFFAYEIWKLWDRNRHQ"
BASE COUNT         2392 a         1416 c         1655 g         2383 t
ORIGIN      
        1 gctgaaggcc cactattcat tcgagaaatc aggcgtattt aacacgaaac aaacatttta
       61 tcatttaagc atacctgaat tatttgaaca cgctttgcag aaaaaagagg gcatattatc
      121 ttctacaggt gcatttagtg ttctcactgg ggcttatacc ggtcgatcac cagaagatcg
      181 tttttttgtc gatgatgaaa ccactcatga tttgattgac tggggaaagg ccaataaacc
      241 gatatcccag gaaatctatg agcatttaaa tgccaaactg gtcagttatc tccaacaaaa
      301 agatgtatac atcgatgacg cctatgtagg aactgaccca gaaaatcgct tggcagttcg
      361 ttttataaac gaatatgcat attcttcaat ctttgttaat aatatgtttt tagaacccac
      421 tgcccaagaa ttacaatctt ttattccgga ttttaccgta ttgtgttttc cacgttttaa
      481 agcaattcct gaaatcgatg aagtgcgatc ggaagccttt gttattatca atttcaaaga
      541 gaaaaaagtg atcatcggtg gcacctccta tggtggcgag ataaaaaaag ctatttttac
      601 tgtgatgaac tatcttcttc cacaaaaagg aattcttcct atgcattgct cggctaatat
      661 cggaaaagaa ggagatgtgg ctatattttt tggcttatca ggaactggta aaacttcgct
      721 ttcagccgat atcgaaagac gtttgattgg cgatgatgaa catggttggt cagatcaggg
      781 aatctttaac tttgaaggag gctgctacgc aaagtgtatc aatctttcca aagataaaga
      841 accacaaatc tgggatgcta tccgctacgg agcaataatg gaaaatattt atcttgaacc
      901 agattcacgt caaccagact atactgacga ccgatatacc gagaatactc gggtgagcta
      961 tccaattgaa tttattccaa atgctttgat accgggaatt gcaaaacacc cacaagtggt
     1021 catattttta actgccgatg ccttcggagt tatgccgcca gtagcgaaat taacgactga
     1081 aaaagcagtt tataacttca ttttagggta taccagtaaa ttggctggaa ctgagagagg
     1141 gatagtagag ccacaaacta ccttttcggt ctgctttgga gcgcccttta tgcctctcaa
     1201 tccaaaaact tacgcccaga tgttgggaaa gaagatatcc cagcatggtt ccagagttta
     1261 tttgattaat accggttgga ttggaggtcc ttacgggata gggaagagaa tcgatattga
     1321 ttatactcgg aagatggtca gggcagcgat tgatggaacg attgatcaga atggctaccg
     1381 tgaagaccca gttttccggc ttttaatacc gaaggaatta cctgatattc ccaatgatat
     1441 cttgaatccg ggaaatcttt ggaacaatca ctctaattat accaacgagg ctcaaaaatt
     1501 gagaaatcgt tttgatgagg tcatgaagaa atacttgcct ctataagaaa tggtgaagaa
     1561 ataaaaagcg gccgccttaa acggccactt ttttaatatc tgtaaatctc ttcgtcagtg
     1621 aggagctgaa agttgtttcg ctctaaaaca ctttgagctc ttggctggct ttcaacctga
     1681 ataatgagta ttgccttctt gccactggcc acaacaaatc cgtaggcatc ctcgatgttg
     1741 atgctgtttt ccgataggac ttgggcaacc cggtggagac cacctggaat atcttccata
     1801 atgactgcaa taacctcttg cagataagaa gggattcctt ggctgcgaag aatttcaaaa
     1861 gctttatggg gttgatcaac taaaacttta ataaccccaa atccattcgc gctggatatg
     1921 gttatcgccc gaatattaat cgaagcttct gtgaacaaac gagttaactt ttctattctc
     1981 cccggtttat tttcagcaaa aattgaaacc tgatgagcca tattgtgtgc gcactccttt
     2041 caagataagt tataattgtc gtttatcaat cacccgcacg gcttttccct cagttgtggg
     2101 aagggtccct ggttcaacta agacgatttc tggagtgact aatgtttctg aacgaatttc
     2161 tgcctgaatg cgttctctca acctttccag cttgggaagg tcaccaataa agagttcggg
     2221 tttaatttct atttcgattt tcatcgtatc gcgaaggtct tttctctcta aggtgatgag
     2281 atagtttgaa ccaacttcag gattgttcat caatacttta tcgatttgta tcgggaaaat
     2341 gtttactccg tggtaaataa acatgtcatc agtccgtcct tgaattcgag atatccggta
     2401 atgggttctc ccacaaggac atggttcatc gatccaataa gccaaatctt tagtacggta
     2461 acggagtata ggagttgctt gtcggcaaag agtagtgaga accatttcac ccagttcgcc
     2521 agctgggcat ggctcgagtg tttttggatt aacaatttct aaaaaataag cgtcttccca
     2581 aacgtgtaat ccagtctgat aggcgcattc aaaggcgact ccaggaccat tcatttcgga
     2641 taaaccatag gaattgaatg cttttgcttg ataaagctcc tcgattcggt gacgggtttc
     2701 ttcgctgtgg ggctcagctc caatcagcat gatcttcaag tttaaatctc gagacgaatc
     2761 taagccttca cttttaatca ggttggaaag caggagggca tagctcggaa taatatgtgt
     2821 cactgtcgtt tgaaagtttt taagaaacca gatttgacgt ttactgtttc ctgctgcaat
     2881 cggtatcacc aaggtaccga ttttttcagc tccataatgc atgccaagac ctccagtaaa
     2941 aaggccatag ctcatcatat tttgaaatat atcattcctc cggaccccca ccccatagag
     3001 tgaacgtgcc accaaatttg accaagagtc aatatcagca gcagtatgat aaataacagt
     3061 tggaacaccg gtcgttccag atgacgagtg aagacgaacc acttcttcca gtggtttggc
     3121 taaaaaacca taaggaaacc cactccgcaa gtcttctttg gacgtaggag ggatgttttg
     3181 cagatccttg gtggttaata ttttttccgg ttgaatatga ttttgtgtga acaatttctt
     3241 ataataggat gtttttgctg cccgctgcat ggtttcttgc aaccgttttg attgcagttg
     3301 gaccaattga tctcgatcca atgtttcgat ctccttttcc caatacaatt gatatcacct
     3361 tcttttttct ttcttcaaat gctcataatg atcaattgga agaaatcatt tcctacaata
     3421 actaaaaaac taaaaaatta aaactcatag tatttatatc acactcaggt ttaaatttac
     3481 catggaacat tcatattttt acgaaaaagc tttgttataa tagtaataaa catcatcaac
     3541 cttattcgga aagctattgg aagtgaaggt gcatatatgg atattcaaat aaatggatta
     3601 gtaagttttt tggctggttt actatccttt ttgtctcctt gtgttttagg tatagctcca
     3661 gcttatgtga gttatatcag tgggttgaat cacttgaaaa agaacgaaag aaagtttttc
     3721 tccacaccat tctttttgtg gttggatttt ctatggtttt tatattcatg ggcgtcggag
     3781 cttctttttt aggaacattt ttgatgaaat ataaagtatg gtttaaccgg atagctgggt
     3841 ttatcattat aatttttggt ttacaaattt tgggaattat aaaaattaag atgttttacg
     3901 ccgaaaagag aatccaacct aaaaaaacct gggcaaagat gagaagcttg atattggggg
     3961 ttactttcgg attaggatgg acaccttgtg ttggtcccat cttaggctcg attcttctct
     4021 atgtatcaac tttagggaac gtattacagg gaggtattta tttatctttt tatgcccttg
     4081 gattagccat accttttatt ttattgggtt taggttgggg atatatgatg agtgttcttc
     4141 ggacgcttca aaaaagaggc catatcattg aaataattag cggaatttta ctgatttttt
     4201 tgggaattat attaatgttt gatcaaatga atgctttcat cgctttcctg ggattggaga
     4261 atatagttcc cggtgaaagc tggtttttga atcaataaca gaaagaattg agggagttta
     4321 tggcaagaaa gtctcaacca gttaaagggt ttggagttgt ctcaaaaaaa ttaagtttat
     4381 ctaaccgagc ctatcgagga attcacgcta ttaaggtaaa gaatattgta ggaagtgtag
     4441 gtcgagtgca agaccttttg agtggtttta gactaaaaaa tcctgatgtt cgctactatc
     4501 ggttaaggaa aattatggaa aaaggtgatg ttgttcctcc aattattgtt tataaagttg
     4561 gcaacgagta ttatgtttta gatggaaacc atcgagtggc aattgctaaa gaactgggaa
     4621 tagaattcat cgatgctgag gtaatcgagt tttttcccag cgaaagaaaa gaaagtcgga
     4681 cccttcgaaa aaagagaatt gaatttgaaa acttaaccgg attaaaggga atcgatttaa
     4741 ctgaacaccg tcattatgat acttttttca attatatcaa tgactatgct caccaaatgg
     4801 aattatttct taaaagaaaa gttgatataa aggaagctgc tttaaactgg tttctttcag
     4861 tctatactcc cgcggttcaa tgtattgtag aaaagggttt agaggatgta tatcaaggga
     4921 aaactttagg agatattttt tgctttattc ttgattataa atggtacaaa agtcaaaaag
     4981 agggttatga tatcggcttt gacaatgcta tgcaaggatt tattgaagaa attcttggaa
     5041 aagaggaacc tgaggagaag aaaaccttga tagaaaagtt cgagggttta ttggaggagg
     5101 ttttaccgtg gatcaaaaag gaataatctg gcatgccctt tcatctcaag aagtcataca
     5161 gcaactggaa accgacttag aaaaaggcat aaaaggagaa gaagcaaaac aaagacttgc
     5221 tgataaagga cggaatgttc ttcctactgg aaaaagaaaa accattttag gtatgtttat
     5281 cgaccaattt aaagatttct tgattctcat cttagttgct gccgctgcca tttcaggttt
     5341 gttgggagaa accaccgatg ccacagttat catcgctatt ttaatcatta atgcagtttt
     5401 aggagttagc caagaaagaa aagctagcaa ggcgctggat gccttaaaaa agatgtcggt
     5461 acccgagtgt gaagttattc gcgatggatt attacagaaa atttcttcag aagaattggt
     5521 tcctggtgat gttattctcc tccacgaagg agactacgtg ccggcagatt tacgtctcat
     5581 tgaagtctat aatctgcgta tcgatgaagc gagcctaact ggcgagtcag tgccagtcga
     5641 aaaaataact gattcaatgg ctgaaaactt acccatctcc gatcgcctta atatggccta
     5701 ttcaggaact attgttgctt atggtcgagc caagggggta gttactgaaa ccggtataaa
     5761 tcgggaaata ggaaaaattg cccaattatt ggagaaagaa gaagaagttg tcacccccct
     5821 ccagaagcga ttggccggtt tgggcaagct gttaggatat ttaactttaa ttgtttgtgg
     5881 agtagttttt gtggttggaa tcatccgagg cgagcaaatt tttgacatgt ttatgacagc
     5941 agttagttta gcagtggccg ctatcccaga aggacttcca gccatagtaa ccattgttct
     6001 tgccttagga gtcacccgaa tgagccaaca caatgctatc gttcgcaaac tacctgctgt
     6061 tgaaaccctg ggaagtgcta cggtgatttg tactgataaa accggaactt tgacacagaa
     6121 ccagatgact gttcaggaag tttttcaaat gaatgaagaa ggggaagagg tccaatcacg
     6181 gatttttatg cccagtgagg ctctcatcca aattggagtt ctttgtaatg atagttcaat
     6241 tgctaataat aatggcaaag tgcaacgctt tggtgatccc actgaattgg cattaatcga
     6301 attagcagaa aaaaatggtt attcagtgaa gaagattcga caagattttc cccgtttaga
     6361 tgaagtccct tttgattcga aacggaaaat gatgtcaact ctgcatgaac tcaatgggaa
     6421 aaggaaaatt ttagtgaagg gcgcccctga cattttaatt acccgttgca actcttatca
     6481 atcaggaggt caaatacttc cccttggcga aaaggatcga ctattcatac ttaacaccat
     6541 tgaagaaatg gccgggaagg ccttacgcgt attggctttt gctgataaag agataccgga
     6601 tcaaaagaaa attagccagg aagatgaaaa tgatctgaat ttccaaggat tagttggcat
     6661 gatagatcct cctcgaccag aagtaaaaca agccttagaa gaagccaata gtgcgggaat
     6721 tgaaactatt atgatcaccg gcgataatct acttacagca agaaccattg ctcaagattt
     6781 gggactggtg caatctgggg acgaagccat caccggacaa gaaatggaaa agctttctca
     6841 agagacctta atggaaaaga tcaaacatct tcgagtattt gctcgggttt ggccagaaca
     6901 aaaattaaac atcgttgagg ctcttcaaaa aaaccaggaa gtagtggcaa tgaccggtga
     6961 tggagtaaat gatgcaccgg ctttgaaaaa agctgatatt ggtgtagcca tgggaatcac
     7021 cggaaccgat gttgccaagg aagtagccga tgtcgtttta atggatgata attttgctac
     7081 tatcgttaaa gcgattgaag aagggcgagt catttttgat aatatcagga aattcgtcat
     7141 gtatctcttg gcttgtaata tcggtgaaat ctttgcaatt tttataccga tattaatcgg
     7201 tcttcaccgt cctctggtcc cagttcaaat attacttatt aatttagtaa ccgatggatt
     7261 gccagctatg gctttaggag ttgatagccc tgaaccagat attatgatga gaaggcctcg
     7321 taatcccaaa gaaggaattc tcaatcccca atccatgaaa ataatcctct ttaattcggt
     7381 gtttatagct atctcagtta tcattgcttt tgttgctggt acaaggatcg gtgggattga
     7441 aaccggccga accatggctt ttgttactct ggcgttttca gagctgcttc gagcttatag
     7501 ttttcgatca gaaaaaagaa atttttggca aattaatctc cgatcaaatc tttatcttat
     7561 tgaagctgca ttattatcgg cagcaattgt gttgattaca gtaatagttc cttctgctgc
     7621 tcaagttttc tctaatgttc cactgaatgg attagaatgg atgtatacta tagtcttttc
     7681 ttttatttca ttttttgctt acgaaatttg gaagctctgg gatagaaatc gtcatcaata
     7741 aaaagaagat cggaatgaac cttagggatt aactttttaa aacgtcattt agactatatc
     7801 ataattccca tcttacacca agaatagctt gaaagtggaa gacaca
//
LOCUS       DAOSOL010000026         7568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_9706_length_7568_cov_3.949953, whole genome shotgun sequence.
ACCESSION   DAOSOL010000026 DAOSOL010000000
VERSION     DAOSOL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_7568_cov_3.949953"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PK171_01265"
     CDS             <1..536
                     /locus_tag="PK171_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HOT04864.1"
                     /translation="PFIEGIQQEKLDYRGVVYLGLMITHHSEVHVLEFNVRLGDPETQ
                     VVLPRLKNDWLDLNMALYRSSLSEVKLEVDTQNLLGVVLTSRGYPGNYEIGKKINGLE
                     DFPNEPQGKVLVFHAGTQRRDNQILTAGGRVLNVCAFGDTLKNAAYNAYLAVGKIGFE
                     NIYYRKDIGYRAFRREE"
     gene            539..1024
                     /gene="purE"
                     /locus_tag="PK171_01270"
     CDS             539..1024
                     /gene="purE"
                     /locus_tag="PK171_01270"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT04865.1"
                     /translation="MSVYVVIGSKHDINYASKCIELLDKFEIEHRLFIASAHRTPEKI
                     DEILQLVEENHIQVLIAMAGYAAHLPGVLAARAVCPVIGVPLDSSSLLGLDALFSIVQ
                     MPAGIPCACVGIGVSGAKNAAILATEILALQNSQLKDRIKAYREEMKEAVIQANRELN
                     L"
     gene            complement(1081..2529)
                     /locus_tag="PK171_01275"
     CDS             complement(1081..2529)
                     /locus_tag="PK171_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT04866.1"
                     /translation="MGYFDSIKAFTAEQVVKAMVSSLRNSSDESIIRLTYLAEKLTPI
                     EYYRDQIRGLRQMFEEKRPQIFLARRVLQEIHPNVRRKLIENLICKSILLGVPKRQKI
                     EKELGCQVPMFFVVSPSMRCNLNCYGCYAGEYRKESDMPIEVLDRIFTEANELGMNFL
                     TISGGEPFLRKEQIDLMEKHSELSFQVYTNGTLIDRDMAKRLAQMGNVYPAISVEGYE
                     AETDARRGKGTYKKIMNAMEALRDEGTLFGFSITATRNNTELVCSDEFMDGLINKGAS
                     FGWYFTYVPIGKKPDMNLMPTPEQRLHLRNQVHHLRYDKPVFIGDFWNDGPYVGGCLA
                     GGRRYFHINNAGDVEPCVFCHFTVDNIKNKSLKEVFSSPFFRAIQENQPYDNNLMRPC
                     MLIDVPEVLRDVVEKYGARPSHEGADSLVKDLKEDIDKYSQAFKDLSDPYWEENYKGT
                     IYYKDYKTERKEYLEKLKETPVPKSHKEKAKV"
     gene            complement(2806..3417)
                     /locus_tag="PK171_01280"
     CDS             complement(2806..3417)
                     /locus_tag="PK171_01280"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT04867.1"
                     /translation="MSYQKSKKEQLLEEIRQEVNSCHLCPLANNRTHTVFGDGNPDAV
                     LMFIGEGPGEEEDRTGSPFVGRAGKLLTQILKSVNITREEVFIANMVKCRPPGNRNPE
                     SQEIDVCFPFLEAQIAIINPTFIITLGSVPTSYFLNTKEPISKIRGIWQEWRGGKKIF
                     PMFHPSFLLRYNDRSRGSPRYLTWEDIKEVKRNYDLIRHSTQS"
     gene            complement(3526..4401)
                     /locus_tag="PK171_01285"
     CDS             complement(3526..4401)
                     /locus_tag="PK171_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04868.1"
                     /translation="MSEIFRSAESNREFDHLYRKGFFKKILGTLKNHKNRLLPLNQIK
                     EIIGTGEEHHLGVKTVEVDKIVGSENRFEDFDRAFLPLNKFNRRKWTSIRSYYEQHES
                     LPVISLYKIGDYYFVRDGHNRVSVAKNSGQLYIDAEVIEINIPKVRFNSSSPEYYFLK
                     LEEQMFREKTGLQNIQITLPGGYREILKLIQCFQCSECPNNQSTREQCQKKIPWKVAV
                     QQWYQNCYFPASEKIERSGIMEKFKDRTLADLYLWFLYNAEALRKAACFIPSRSHHKA
                     IRRKPQNFFFTKANL"
     gene            complement(4358..5014)
                     /locus_tag="PK171_01290"
     CDS             complement(4358..5014)
                     /locus_tag="PK171_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT04869.1"
                     /translation="MKILAVSDHIDPRVYSNLCRERFQNVSCVISCGDLPEHYLDFIV
                     SCLNVPLFFVHGNHDPSAGKKSLAGGYNLDGKVINFQGILLAGLEGSLWYNGNIHQYS
                     QRAMYQKYLALLPQLYWAKMRYHRYLDVLVTHSPPGGIHESDDPVHKGFKVFNHMIKK
                     FQPRFHLHGHTHLYDRNQSYQDLLYQTIVINCYNYRIIDLGRGKDVRDFSISGVKSRV
                     "
     gene            5278..6837
                     /locus_tag="PK171_01295"
     CDS             5278..6837
                     /locus_tag="PK171_01295"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOT04870.1"
                     /translation="MDEKHHYGVVDNLDKLFLVLPEHILKALEEKENSDELIEIVMDL
                     GRSPEARFTNGFCVLIESPVERSDIEYTLGRVGMFTRDNRAGIERTLHRISCIRNRVG
                     NVVGLTLRVGRAVYGTIDIVRDIIVSGKNILFLGPPGVGKTTKLREVARVLSDEFQKR
                     VIVVDTSNEIAGDGDIPHPAIGRARRMQVPSPERQHDVMIEAVENHMPEVIIVDEIGR
                     EEEARACRTIAERGVQLIATAHGNSLRNLLLNPTLTDLVGGIQSVILSDEESRRRGTQ
                     KAVLERKAIPTFDVVVELRDRDILGVYRNTAEAVDVLLRNYDPRPEVRKKTKDGAIEV
                     IKEIEEFETVEGVYNLSDWNHPQYSEGKMLRVFLFAVSRNYMERAITQLKATLEVVED
                     INEADIVLTLKSRYRKKTNRLREAENRGMPVHVIRSNTYSQVLRFVKDLFGLSDSFEK
                     SGETGLIEAEKAARQVLRENEPIELTPRNAFVRRLQHKIAERYNLFSQSIGEEPFRRV
                     TIYPRNLDDNR"
     gene            6834..7502
                     /gene="tmk"
                     /locus_tag="PK171_01300"
     CDS             6834..7502
                     /gene="tmk"
                     /locus_tag="PK171_01300"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOT04871.1"
                     /translation="MRHFPGLFITFEGIEGTGKSTHAKHLFAFLKEKRYPVLFTVEPG
                     GTEIGNQIRSILLHPQTGEVDAVTELFLFEASRREHVLQVIQPALQIGKIVLCVRFCD
                     STIAYQGYGRGLSVEMIQYLNSLATNGLVPDLTLLLDIDPEEGLRRSFHHTAPQELRF
                     EEEFMKKKDVLDSIRRGFFKIAEGEPNRFQVISTVESKDVVFERIKNYVLAAIQDKKR
                     RNVP"
BASE COUNT         2301 a         1396 c         1542 g         2329 t
ORIGIN      
        1 atccatttat tgaaggaatt cagcaagaaa aactggatta tcggggagtt gtctatctgg
       61 gtttgatgat tacccatcat tctgaggtcc atgttttgga atttaatgtt cgtttgggag
      121 atcctgaaac ccaagtggtc ttaccccggc ttaagaatga ttggctagac ttaaatatgg
      181 ctctttaccg gagctcgctg tcagaggtta aacttgaagt ggatactcaa aaccttttgg
      241 gggtggtttt gaccagtcgt ggttatccgg gaaactatga aattgggaag aagattaatg
      301 gtttagagga ttttcccaat gaaccacagg gaaaggtact ggtttttcat gccggtaccc
      361 agagaagaga taatcaaatt ttaacagctg gtgggagagt attaaatgtg tgcgcctttg
      421 gtgatactct taaaaatgca gcctacaatg cctatttggc ggttgggaaa atcggttttg
      481 aaaatatata ttatcgtaaa gatattggat atcgtgcttt tcgtagggag gaatagctat
      541 gtcggtttat gtggttattg gaagtaaaca tgatatcaat tatgcctcga agtgtattga
      601 acttcttgat aagtttgaaa tagagcatcg actttttatc gcttctgccc atcgaactcc
      661 ggaaaaaatt gatgaaatat tacaattagt agaagagaat catatccagg ttcttatagc
      721 tatggctgga tatgcggctc atctccccgg agttttggct gcccgtgcag tgtgtccagt
      781 tattggtgtc cctctcgatt cttcctcact tttaggattg gacgcattat tttccattgt
      841 tcaaatgccg gctggcatac cctgtgcctg cgtggggata ggagtaagcg gtgcaaaaaa
      901 tgcagctatc ttagcaactg agattttagc ccttcaaaat tctcagttaa aagatagaat
      961 aaaagcttat cgagaagaaa tgaaagaagc ggttatccag gctaatcggg aattaaacct
     1021 ttgataattt aatattatca ataaaaaaag cctccctaaa aaaggaggct ttttttatat
     1081 ttagactttt gctttctcct tatgggattt agggactgga gtctctttta gtttctcgag
     1141 gtactctttt ctttcagttt tataatcttt gtaatagatg gttcctttgt agttttcctc
     1201 ccagtatgga tcggataaat ctttaaaagc ttgagaatat ttatcgatat cctctttcag
     1261 atctttgaca agactgtcag caccttcatg gctgggtctt gccccatatt tttctactac
     1321 atcccgaagt acttcaggaa catcgatcaa catacagggg cgcattaaat tattatcata
     1381 tggttggttt tcttgaatag cccggaagaa tggagaagaa aaaacctctt tcaaactttt
     1441 attcttgatg ttgtcaacag tgaaatgaca aaatacacaa ggttcaacat caccagcatt
     1501 gttgatgtgg aaatatcgtc gaccaccggc gagacaacca ccaacgtatg gtccatcatt
     1561 ccaaaaatca ccgatgaaaa ctggtttgtc atatcgtaga tggtgaacct gattacggag
     1621 atgaagacgt tgttcaggtg ttggcatgag attcatgtct ggttttttcc caattgggac
     1681 ataggtaaag taccaaccaa aactcgctcc tttgttgatt aaaccatcca taaattcgtc
     1741 actacaaacc aattcagtgt tatttcgggt ggcagtgatg gaaaatccaa agagtgttcc
     1801 ttcatcacga agagcttcca tagcattcat aattttcttg taagtacctt tgccgcgccg
     1861 agcatcagtt tcagcttcgt aaccctcaac actgatggca ggataaacat tacccatttg
     1921 agctaaacgc tttgccatat ctcgatctat aagagttcca ttagtataaa cttggaagga
     1981 gagttcgctg tgtttttcca ttagatcaat ttgttctttg cggagaaaag gttctccacc
     2041 agaaatggtg aggaaattca tgcccagttc attagcctca gtaaaaattc tgtcaaggac
     2101 ttctattggc atatcgcttt ctttgcgata ttcgccggca taacatccat aacagttgag
     2161 gttacaacgc atacttggac tcactacaaa aaacataggt acttgacagc ctaattcctt
     2221 ttcaattttt tgccttttag gaactcctaa aagaatgctt ttacagatga gattttcaat
     2281 aagttttcgt ctcacgttgg gatgaatttc ttgaagtact cgccgtgcca aaaatatttg
     2341 aggtcttttt tcctcaaaca tctggcgaag tccacgaatc tggtcacgat aatactcaat
     2401 tggtgttagt ttttcagcta aataagttaa acggataatt gattcgtcgg aggagtttcg
     2461 taaagaacta accattgctt taacgacctg ttcagcggta aaggctttta tagaatcaaa
     2521 gtaacccata ttatgaacct ccctcatacg acgtgtttat tctgtataac atataaaacc
     2581 accccttatg gagtatgata agtaaccaat aaaacaaact attttatcaa aagctatctt
     2641 tttcgaaaac actcttacaa ttttatgtat tcccgtttat tattttttac gtaattaaag
     2701 ataagaaagt tttaggatac ttaaaaatac aattatagaa taatttaaag aaaaactaaa
     2761 aactaagtac tttactctta tatattatag caaaaaaatt gaatttcaac tttgggtact
     2821 atggcgaatt aaatcatagt tccttttcac ttctttgata tcttcccagg taagataccg
     2881 gggtgaaccc cgggaacgat cattataacg gaggagaaaa ctgggatgaa acatgggaaa
     2941 tatttttttg ccacccctcc attcctgcca gatacctcga attttgctaa tcggttcttt
     3001 ggtattgagg aaataactag tcggaacact gcctaaggtg atgatgaaag ttggattgat
     3061 aatggcaatt tgtgcttcta gaaagggaaa gcagacatct atttcctggg attccggatt
     3121 tcgattccct ggcggtcggc atttcaccat gttagcgata aacacctctt ctcgagtgat
     3181 attaaccgat tttaaaattt gggtcaggag cttcccggct cttccaacaa aggggcttcc
     3241 ggttcgatcc tcttcctctc cgggtccttc cccaataaac atgagaactg catcaggatt
     3301 tccgtcacca aatacagtat gggttcggtt attggctaac gggcacaaat gacaactatt
     3361 aacttcctgg cgaatttctt ctaaaagttg ttcttttttc gacttttgat atgacatctt
     3421 tcgctatcct actgtaaaaa attaaaaacc tttcaaatac cagctgagat tatagaaaaa
     3481 tattgaacgc tttactttag acatagtata ttgatatgga actttttaca aatttgcttt
     3541 ggtaaaaaag aaattttgtg gttttcttcg aattgcttta tgatgagatc gacttggtat
     3601 aaaacaagct gccttgcgta gggcttcagc attatagaga aaccataaat agaggtcagc
     3661 taaagtacga tctttaaatt tctccataat tccgcttctt tctatttttt cagaagccgg
     3721 gaaataacag ttttgatacc attgctgaac ggcaactttc caaggtattt ttttttggca
     3781 ttgttctcga gtactttgat tatttggaca ctcagaacat tggaaacatt gaattaactt
     3841 gagtatttct cgatacccac cgggaagagt aatttggata ttttgtaagc cggttttctc
     3901 ccgaaacatc tgttcttcta actttaaaaa atagtattca ggagaagatg aattgaagcg
     3961 tactttcggg atgttgattt ctattacctc agcatcaata tagagttgcc cggaattttt
     4021 tgctactgaa actcgattat gaccatctcg gacaaaataa taatcaccga ttttatatag
     4081 agagataacc ggtagagatt catgttgttc ataataggag cgaatactcg tccattttct
     4141 ccgattgaac ttatttaatg gcaaaaaagc tcgatcaaaa tcttcaaaac gattttcact
     4201 cccgactatt ttatcaacct caacggtttt cacgcccaaa tgatgttctt ctccagttcc
     4261 aatgatttct ttaatttgat ttaaagggag caaacgattt ttgtggtttt tcagagtacc
     4321 aagaattttt ttaaagaaac cttttcgata gagatgatca aactctcgat ttgactccgc
     4381 tgatcgaaaa atctctgaca tctttacccc ttcctaaatc tataatacga taattataac
     4441 aatttattac tatagtttga taaagaaggt cttgatagct ttgatttcgg tcgtataaat
     4501 gagtatgtcc atgaaggtgg aatcttggtt gaaatttttt aatcatatga ttaaatactt
     4561 taaaaccttt atgaactgga tcatcacttt catgaatgcc accgggagga gagtgagtga
     4621 ctaaaacgtc aagataacga tgataacgca ttttagccca atatagctga ggcaagagag
     4681 caaggtattt ttgatacatc gctctttggg aatattggtg aatattgcca ttgtaccata
     4741 gagatccttc caagccggca agcaggatcc cttgaaaatt gataaccttt ccgtctaaat
     4801 tataaccacc tgccaagctt ttttttccgg cagaaggatc atgatttccg tgaacaaaga
     4861 ataaagggac gttcagacag ctgactataa aatccagata atgctcggga aggtctccac
     4921 aggagataac acaactcaca ttctgaaaac gctcccgaca taggttacta taaactcgtg
     4981 gatcaatatg atcgctcacg gctaagattt tcattccgtt tattatacct aaaggattaa
     5041 tatttgataa gtaatacagg tttttaaaac aacttagaat gaattttatt cctaaatgat
     5101 aatcttttac caagaggaaa ggagaataga aaaaaagtac catttcttct tgatattttt
     5161 tggatcgata agttttttaa ttttaatgta aaaatgaaaa aacagcataa gagggaatga
     5221 catccttaat gatataatgc tgaatgaaat cttgtaggaa ggaactgtga agttcgaatg
     5281 gacgaaaagc atcattacgg tgtagttgac aatcttgata aactgttttt agttttacca
     5341 gaacacattc tgaaggctct tgaagaaaaa gaaaactctg atgaactgat agaaattgtt
     5401 atggacttgg gccgttctcc agaagcgcgg tttaccaatg gcttctgtgt tctaattgaa
     5461 agtcctgttg aaagaagtga tattgaatat actttaggta gggttggtat gtttacccgt
     5521 gataaccgag ccgggatcga aagaactctc caccgaattt catgcatccg gaatcgggtt
     5581 gggaatgttg taggtcttac tttgcgggta ggacgcgctg tgtatggtac cattgatatc
     5641 gtacgagata tcattgtgtc agggaaaaac attttatttt taggcccacc aggagttggc
     5701 aaaaccacca aacttcgaga ggtagctcgg gtgttaagcg atgaatttca aaaaagggtc
     5761 atcgttgtcg atacctcaaa tgaaatagct ggtgacggtg atattcccca tccagctatt
     5821 gggcgagccc gtcgaatgca agtaccatca ccagaaaggc aacatgatgt gatgattgaa
     5881 gccgtggaaa accacatgcc ggaagtcatt attgttgatg aaatcggacg tgaagaagaa
     5941 gcccgggcct gtcgaaccat tgctgaaaga ggagttcaac tgattgcaac cgctcatggt
     6001 aattccttac gaaacctttt attgaacccc actcttactg acttagtggg tggcattcaa
     6061 tcggttattc tcagcgatga agaatcaaga cggcgtggaa cccaaaaagc tgttttagaa
     6121 cggaaggcaa tcccgacttt tgatgtagta gtagaactca gggatcgaga tattttagga
     6181 gtctatcgta atactgccga agcggtagat gtccttttaa ggaactatga tcctcgtccg
     6241 gaagtaagga aaaaaaccaa agatggtgcc atcgaagtta ttaaagaaat cgaggaattc
     6301 gagaccgtcg agggtgtata caacctatcc gactggaacc atccgcaata ctcagaagga
     6361 aaaatgttac gggtatttct ttttgctgtg agtcgaaatt atatggaaag agctataacc
     6421 cagcttaagg caacccttga agttgttgaa gatatcaatg aagccgatat cgttttaact
     6481 ctgaagagtc gttatcgtaa aaaaaccaat cggcttcgag aggctgaaaa tcgagggatg
     6541 ccggttcacg ttatccgtag caacacctat agccaagtgc tccgttttgt caaagatctt
     6601 tttggcttgt ccgacagctt tgaaaaatca ggagaaaccg gtttgattga agctgaaaag
     6661 gcggcaaggc aggtcctgag agaaaacgaa ccaatcgagc tcactcctcg gaatgctttt
     6721 gtccgaagac ttcaacataa aatcgcagaa agatataatt tattctccca gagtattggt
     6781 gaggagcctt ttcgcagggt aaccatttat ccacgaaatc tggatgataa tcgatgagac
     6841 attttccggg attgtttata acctttgaag gaatagaagg aacaggaaaa tcgacccatg
     6901 ccaagcacct ttttgctttt ttaaaggaaa agagatatcc agtccttttt actgttgaac
     6961 caggtggaac ggaaatcggc aatcaaattc gatcaattct tcttcatcct caaaccgggg
     7021 aagttgatgc tgtaaccgag ctttttcttt ttgaagcctc gcgtcgggaa catgtcttac
     7081 aagttattca gccagcctta caaatcggaa agattgttct ctgtgtgcgt ttttgtgact
     7141 caaccattgc ctaccaggga tatggtagag gtttgtcggt tgagatgatt caatacctca
     7201 atagtttggc caccaatgga ctggtgcctg atttaaccct tcttcttgat atcgatcccg
     7261 aagaagggtt aagacgatcc tttcatcata ccgctccgca agagctgcgt tttgaagagg
     7321 aattcatgaa gaaaaaggac gttttagatt ccatacggag aggttttttt aaaatagctg
     7381 aaggagaacc aaatcgattt caggtgatat ctactgttga atcaaaagac gttgtttttg
     7441 agagaataaa aaattatgta ttagcggcta ttcaagacaa aaaaaggagg aatgtgccgt
     7501 gaagccaacc tctttattaa tatgtgtcgt tcgagatcaa gacgctttga agctggttga
     7561 tatcctca
//
LOCUS       DAOSOL010000027         7402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10044_length_7402_cov_3.753369, whole genome shotgun sequence.
ACCESSION   DAOSOL010000027 DAOSOL010000000
VERSION     DAOSOL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10044_length_7402_cov_3.753369"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..354)
                     /locus_tag="PK171_01305"
     CDS             complement(37..354)
                     /locus_tag="PK171_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HOT04872.1"
                     /translation="MNNRVVRFFWGKPTLLGEILEEYLEKEGLVETIKIHQLIELFGE
                     FFPNLNQLCQCEGYRNGVLFLSITDPIFNLEVQKLIPDICRIYQEKGLMVKKVKIRGI
                     GKK"
     gene            complement(347..1441)
                     /gene="recF"
                     /locus_tag="PK171_01310"
     CDS             complement(347..1441)
                     /gene="recF"
                     /locus_tag="PK171_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOT04873.1"
                     /translation="MLIQELKCINWRNLEKLNLSFSNHLIILQGKNAQGKTNLLEAIY
                     FLSRGRSFRRAKDQEMVTWGESYSYLGAKVGEDGTYFWKEVSIQPGQKKEWKINGHPG
                     SGRRGIWLVGYFPSDHEIVAGPPQNRREYIDEAIGFIYFAHAALVKQYEGLLSRRNYL
                     LREHASDDLIETYTEKLIHVGKKIIQGRLEYLRLYTPLIKEFYYRLGMNNQSLTLRYQ
                     PFGYHLDNEGGIHMEKGFEMVKEEEREREITIIGPHRDEIVFEKNGREFRTFGSQGEK
                     KSLALSIKLAEMSVIQLIKKSKVIMIFDDVFSELDQSHQDLLLEEILNQGQVFISTTQ
                     ENDDWARKRKNRVTVLNVEKGKIIMGKCNE"
     gene            complement(1445..2557)
                     /gene="dnaN"
                     /locus_tag="PK171_01315"
     CDS             complement(1445..2557)
                     /gene="dnaN"
                     /locus_tag="PK171_01315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT04874.1"
                     /translation="MKATVSLKDLKKAVDHVSVCVPSRSSIPVLTGLLIEAENKETIK
                     ILSNDMEMGIIAKCQAQVEESTSIVLPGKLLIGIARGMVGETVTINSLSDSMVTELRC
                     GKSIFKLTGYPPENFPVFPPFQKDKYFTVKSEQLRTGYNRTAFAASNDETRGPLTGVL
                     IEGKESFLYLAATDGHRLSMHKIEKINDLVGEKIRFIVPSKVAAAIMRAMVGERVDVI
                     PGQGEIMFDMDTVTIYSRLIEGEFPEYESVLPTEFTTTVTMNRKTFLEGIERAALVTT
                     PEEETIYLQFNNRKLMMTCESQGMGIAKEEMEITIEGQAVNIAFNSRFLIDCIRVAPW
                     ENISIGINGEVAPVLIFGDNDEFRHLIMPLKVREEE"
     gene            complement(2754..4112)
                     /gene="dnaA"
                     /locus_tag="PK171_01320"
     CDS             complement(2754..4112)
                     /gene="dnaA"
                     /locus_tag="PK171_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT04875.1"
                     /translation="MNDVSSTLDGLWIKTISYLEKQLSVPEYAAWIETIHPISFRDNE
                     LILSVPSGLAREKVEQKYLDLLRFSFSKTVGKDISDLKVILSVDNYPPISQNPTPILV
                     SQSSDWEKPNLNPRYSFSSFVVGNSNKFAHAAAVAVAESPAKSYNPLFIYGGVGLGKT
                     HLMHAIGHQVLKKNQSARIVYSSSEKFTNELINSIRDDKTEDFRQKYRNVDVLLIDDI
                     QFLAGKERTQEEFFHTFNTLHDALKQIVLTSDRPPKEIPTLEDRLRSRFEWGLLADIQ
                     PPDLETRIAILRKKAETEHIDIPDEVILFIADRIPSNIRELEGALVRIKAYCTLNQIS
                     PNPEIAEEILKDILPKTVAKKISVSTIQRVVSEHYSIKPGLMRAKKRTREVAFPRQIA
                     MYITRSLTSYSLPAIGEEFGGRDHTTVLHAYEKIKTDMENNPQLSQEISGIIEKIQRE
                     GS"
     gene            complement(4637..4900)
                     /locus_tag="PK171_01325"
     CDS             complement(4637..4900)
                     /locus_tag="PK171_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04876.1"
                     /translation="MIRPGVGATAAYILFLTWQEFMFALTFITSQDKRTLPVGIFDFV
                     GQYETNWGNLMAASVLICIPVFILFLFVQKQLVGGLTQGAVKG"
     gene            complement(4988..5965)
                     /locus_tag="PK171_01330"
     CDS             complement(4988..5965)
                     /locus_tag="PK171_01330"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOT04877.1"
                     /translation="MRQITYREAIHEVLDYELETNPDVFLLGEDIGPLGGAFSVTKGL
                     WEKFGNERVRNTPLSEIAIVGAATGAASVGKRPIAEIMFCDFLFVAGDQLVNQTPKMR
                     FMFGDQCQLPLVIRTTIGAGQSAAAQHSQSLEGMLAHIPGFITVVPSTPYDVKGLLKT
                     AIEDNNPVLFYEHKGLYNLKGDVPKEMYSIPFGVADVKRTGSDVTIIATAETIHKSLR
                     VAERLQNEGVSIEVVDPRTLVPLDIETLVHSVKKTHRAVIVHEAHLSYGPGAEIAARL
                     ADEAFDYLDAPIKRVGAKDIPIPMAPHLESYVIPQEDDIEKAVRSVINF"
     gene            complement(5981..6940)
                     /locus_tag="PK171_01335"
     CDS             complement(5981..6940)
                     /locus_tag="PK171_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOT04878.1"
                     /translation="MIEKDLARILLITMKKIRIFEEEAVKLFAAHELPGWLHSYIGEE
                     AVAAGVCLALNPSDYIASTHRGHGHCIAKGVSLRKMMAELYGKETGYCKGRGGSMHIA
                     DFSLGMLGANGIVGGGIPIATGAALGSQMKKDGKVTVCFFGDGASNQGAFHESLNLAS
                     AWKLPIVYVCENNLYAETTPQAEHQAIHNIADRAVGYGMPGVTTDGMDVISVYKNAQT
                     AIERARSGKGPTLLEAKTYRYRGHWEGDPQPYRTKADIEEWKKKDPIVNFERFLLDQK
                     FITSNEIEALTEEIVNEINDAIEFGRTSPYADPQACLSTLYIH"
BASE COUNT         2147 a         1570 c         1336 g         2349 t
ORIGIN      
        1 cgtcttttcg ataaagggaa aacaaattta atttttctat tttttcccta tcccacggat
       61 ttttactttt tttaccatta atcctttttc ctgatagatt ctgcaaatgt ccggtattaa
      121 tttttgcact tcaagattga atattggatc ggttatactt aaaaataaaa caccattacg
      181 atagccctca cactggcaga gctggtttaa attcggaaaa aattcaccaa ataattctat
      241 aagttggtga attttaattg tctcgactaa gccctctttc tctaagtatt cctctaaaat
      301 ctcgccgagc aaagttggtt ttccccagaa aaaacgaacc actcggttat tcattacatt
      361 tccccattat aatttttcct ttttcaacat ttaacactgt tactcgattt tttcgttttc
      421 ttgcccaatc atcattttct tgggtcgtgg atatgaacac ttgcccttgg tttaagattt
      481 cttctaacaa tagatcttgg tgagattgat caagctcaga aaaaacatcg tcaaaaatca
      541 taattacttt gcttttcttg attaattgta tcacagacat ctcagctaat ttaattgata
      601 aagcaagaga ttttttctct ccttgggatc caaaggttcg aaactcccgt ccattcttct
      661 caaaaactat ttcatcacga tgcggtccaa taatagtaat ctctctttct ctttcttctt
      721 ccttaaccat ttcgaagcct ttttccatat ggattccccc ctcgttatcc aagtgatatc
      781 caaacggttg gtatctgagt gtaagcgatt ggttgttcat tcctaaacga taataaaact
      841 ccttaattaa aggggtgtag agtctcaaat attcgagtct tccctgaata atcttctttc
      901 caacatgaat gagtttttct gtataggttt caattaaatc atcacttgca tgctcacgga
      961 gtaaataatt tctacgtgat agtaatccct catactgttt taccaaggca gcatgagcga
     1021 aatatataaa ccctattgcc tcgtctatat attctctcct gttttgtggt ggacccgcta
     1081 cgatttcatg gtcactgggg aaataaccga ctaaccaaat acctcgcctt cctgatccgg
     1141 gatgaccgtt tattttccat tctttttttt gacctggttg tattgacact tccttccaga
     1201 agtaggttcc gtcctcccca accttagctc ctagataact ataactttct ccccaggtga
     1261 ccatctcctg gtcttttgct cttctaaatg agcgacctct tgataaaaaa tatatcgctt
     1321 ccaagagatt ggtttttcct tgagcatttt ttccctgtaa aataattaag tggttggaga
     1381 aacttaagtt taatttttcc agatttctcc aattaataca ttttaattct tgaatcaaca
     1441 tgccctattc ttcttctcta acttttagtg gcatgattaa atgtcggaat tcgtcattat
     1501 cgccaaatat caaaactggt gccacttcac cattaatacc aatggaaata ttttcccatg
     1561 gtgcaactcg aatacaatca attaaaaaac gtgaattaaa tgctatgtta acagcttgtc
     1621 cttctatggt aatttccatt tcttcttttg ctattcccat tccctggctt tcacaggtca
     1681 tcatcaattt tctattgtta aattgtaaat aaatcgtttc ttcttctggt gttgtaacta
     1741 atgcggctct ttctattccc tcgaggaaag tttttcgatt catagttaca gttgttgtaa
     1801 actcggttgg caaaactgac tcatactcag gaaattctcc ctcaataagt cgactatata
     1861 ttgttaccgt atccatatcg aacataattt ccccttgacc aggaataaca tcaactcttt
     1921 cccccaccat cgctctcatg attgccgctg ctactttcga aggcacaata aatcgtatct
     1981 tttctcccac taaatcgttt attttttcta ttttatgcat actcaaccta tgaccatcgg
     2041 ttgctgctag atacaaaaat gattcttttc cttctataag aacaccagtg agtggaccac
     2101 gagtttcatc gtttgatgcc gcaaaagctg ttcggttata accagttcga agctgttcac
     2161 tcttgacagt aaaatattta tctttttgaa aaggaggaaa taccggaaaa ttttccggtg
     2221 ggtaaccggt taacttaaaa atactcttgc cacaccttaa ctcagtgacc atactatctg
     2281 acagtgaatt tattgttacc gtttctccca ccattcccct cgctattcca attaacaatt
     2341 taccagggag tacaatactg gtactttctt ccacttgagc ctgacacttt gcaataatac
     2401 ccatttccat gtcatttgat aatatcttaa tcgtttcttt attttcagcc tcaattaata
     2461 acccagttaa aacaggaata gaactgcgag agggaacaca aaccgaaaca tgatcaaccg
     2521 ccttctttaa gtcttttaaa ctaactgtag ctttcatgga tttatccccc tgttcgatag
     2581 ttgtccacaa taatcttatt actattacta ctctttttat ttatttaaaa aaatagaagg
     2641 agtagtagta gagaaaaaat ctgtggatat ctctcctttt tcctataact ttgacaaaaa
     2701 caatgataaa agattaggtt ttattttgtg aagtgattgt ggaaggaatg tgattaactt
     2761 ccttctcgct gtattttttc tattattcca ctgatttctt gtgaaagctg tggattattt
     2821 tccatgtcag ttttaatttt ttcataagca tgaagaacag ttgtatgatc tctcccacca
     2881 aactcttctc caatagccgg gagggaataa ctggtcaggg agcgggtgat gtacatggct
     2941 atctgcctcg gaaaggctac ttccctggtt ctcttcttag ctcgcattag tcctggtttt
     3001 attgagtaat gttctgacac gaccctctgg atagtgctca cactgatttt cttagcaaca
     3061 gtttttggga ggatatcttt taatatttcc tcagctatct caggattagg actgatttga
     3121 ttcaaagtgc aataagcctt aatacgaacc agagcacctt ctaattctct aatgtttgag
     3181 ggaatgcgat cagcaataaa aagaataact tcatcaggga tatcaatatg ttcagtttcc
     3241 gcctttttcc tgagtatagc aatacgcgtc tctaagtctg ggggttggat gtcggccaac
     3301 aatccccatt caaaacgaga acgtaatcga tcttcaagag taggtatttc ctttggtgga
     3361 cgatcgctgg taagaacaat ttgctttaaa gcatcatgga gagtattaaa agtatgaaaa
     3421 aattcttctt gagttctctc tttgccggct aaaaattgaa tatcatcaat aagaagaacg
     3481 tcaacattac ggtatttttg acgaaaatcc tcagtcttat cgtcccttat ggaattgatt
     3541 aactcattgg tgaatttttc agaagaggaa tagacaatac gagctgattg attttttttt
     3601 aatacctggt gcccaattgc gtgcattaag tgtgtttttc ctaaaccgac tcccccatag
     3661 atgaaaaggg gattgtacga tttagcaggg ctttctgcca cggcaaccgc agcagcgtgg
     3721 gcaaacttat tactattacc gacaacaaaa gaagaaaaac tataacgcgg gttaaggttg
     3781 ggcttttccc aatcgctaga ttgagaaaca agaataggag taggattttg agaaattgga
     3841 ggataattat ccacagaaag tataactttc aagtctgaaa tatctttccc aacggttttt
     3901 gaaaaagaaa agcgaagtaa atccaaatat ttctgttcga ccttctcccg tgccaagccg
     3961 gaaggaacac taagaattaa ttcattgtca cgaaaggaaa taggatgaat tgtttcaatc
     4021 caagctgcat attctggaac cgataattgc ttttccaaat aagagatggt ttttatccac
     4081 aacccatcta atgtgcttga tacatcattc ataaaaagcc accccaactt atttaattgt
     4141 aattcacaag gatatccaca aggaattcta accccctgga tgagggcaat agaaaattgc
     4201 aaaagcgcat gacaaggggt tatatgagta agaagagaac aataaaatgt tttttttaaa
     4261 attttactct cgtcctatta aaccataaag gaaggcttct tggcaagtta tccacaagtt
     4321 gttaacaggt gtggataact ttattgttat aacttttccc ttggtgttga atagtatatc
     4381 atgaggaagc tatttatcga aaaattgaat agatgataaa tgaggataaa aacttgagga
     4441 aatcgacttt tccacagata aaaccaagat attcacagat tcttgtggat atatactctt
     4501 caactaaaaa agggaccagt ggtgactttt aatgttaccc tatttttctc ccatcaagca
     4561 ttgatgctcc ttagaaggga aaaaaattaa atattgaaag cgatttggaa aaatcccact
     4621 catagaaaaa acaaccttat ccttttactg cgccttgagt gagcccacca accaattgct
     4681 tttgtacaaa tagaaagaga ataaaaaccg gaatacagat gagaaccgag gcggccataa
     4741 ggtttcccca attggtttca tattgaccaa caaagtcgaa tataccaacc ggaagagttc
     4801 ttttgtcttg gctggttatg aaagttagag caaacataaa ttcctgccac gtaaggaaaa
     4861 gaatataggc agcagttgcg ccaacaccag gtcgtatcaa tggaacgact attttctaaa
     4921 cttctccttt ttttcctctc ctactaaggg aaaaggacga tgttgccgat atcccgaatt
     4981 gaagacttta aaaattaatc actgaccgaa cggctttttc aatatcgtct tcttgaggaa
     5041 tgacataact ttccaaatgc ggagccattg ggatcgggat gtctttagca ccaactcgct
     5101 taatcggagc atcaagatag tcgaaagctt catcagcaag gcgtgctgca atttcagcac
     5161 ctggcccata acttaaatgg gcttcatgaa caatgaccgc ccgatgagtt ttctttactg
     5221 aatgaaccaa ggtttcaata tccaatggaa ccagagtgcg gggatcgaca acttcaatac
     5281 taactccttc gttttgtaaa cgttcggcta cccgcaatga cttgtgtatt gtttctgcgg
     5341 tggcaataat cgttacatca ctcccggttc ttttaacgtc tgcaactcca aaaggaatgg
     5401 aatacatttc ttttgggaca tcacctttga gattgtatag tcctttatgt tcgtaaaaga
     5461 gcaccgggtt attgtcttca atagcagttt taagtagacc ctttacatca tagggggtcg
     5521 aaggaacgac ggttataaaa cctgggatgt gggctaacat cccctctaag gattgagaat
     5581 gttgggctgc tgcggactga ccggcaccaa tagtagttcg aatcaccaaa ggaagttgac
     5641 attggtcgcc aaacatgaat cgcatctttg gtgtttggtt aaccaactga tcacctgcaa
     5701 caaaaaggaa gtcgcagaac ataatttcgg ctatcggtcg ctttcctacc gaggcagcgc
     5761 cagtggcagc acccacaatt gctatttcag acaaaggagt gttacggact cgctcatttc
     5821 caaatttttc ccataatcct ttggtaacgc taaatgcccc gcccagaggt ccaatgtctt
     5881 ctcccaataa aaaaacatcg ggattggttt caagttcata atctaaaact tcatgaatgg
     5941 cttcacgata agtaatttgt cgcatgattc attctccttc tcagtgtata taaagtgtag
     6001 agaggcaagc ttgtggatcg gcataaggcg aggtacgacc gaattcaatg gcatcattaa
     6061 tttcattgac aatctcctcg gtaagagctt caatttcatt ggaagtgatg aatttttggt
     6121 cgaggagaaa cctttcaaag tttacgatgg ggtctttctt tttccattcc tcgatatcag
     6181 ccttggtccg gtatggctga ggatcacctt cccaatgtcc gcggtatcga taggttttag
     6241 cttccaatag ggtcggtcct ttacctgacc gagccctttc aatggccgtt tgagcgtttt
     6301 tgtaaactga aatgacatcc atgccatcgg tggttactcc cggcatcccg taacccaccg
     6361 cccggtcggc aatattatgg attgcctgat gctcggcttg tggtgtggtt tcagcataaa
     6421 gattgttttc acatacatat acaatgggga gtttccaggc tgaagctaaa ttgagggatt
     6481 catgaaaagc accttgattc gaggctccat cgccaaagaa gcacaccgta accttgccgt
     6541 cttttttcat ctgacttccc aaggccgcac cagtagcaat gggaataccc cctcctacaa
     6601 ttccattggc acccagcatt cccagggaaa agtcagctat gtgcatggat ccccctcttc
     6661 ccttgcagta gccggtttct ttgccatata actcagccat cattttccga agagacactc
     6721 ctttggcaat acagtgacca tggcctcgat gggtgctggc gatataatca ctagggttta
     6781 gcgccaaaca aacgcctgca gcgacagcct cttccccgat ataactatga agccagcccg
     6841 gcaattcatg agcagcaaat aactttactg cctcctcttc aaagatccgg atctttttca
     6901 tggttatgag aaggattcga gccaaatctt tttcaatcat aaacgctatt ctcctttatt
     6961 tgatattagt ttgtttcctt tatgatactt aattttttca aaaccccttt ttagaggaga
     7021 tttgaaaaaa aattattgct gctcaccctg accctctccc gctgaggggc gagggaaaaa
     7081 ataaataaaa gaatgaaata gttccttctc ccttgatggg ataaggtgag ggtgaaagct
     7141 caggattcga catgccatgg caggtcgcta tattaattaa agaatgggca caatacacaa
     7201 tatattgtgc cccgacattt tatattcttt ggtaggggct tgatttatca tgcccgtgga
     7261 ttttcaggat agaccctcat gctgcctaga agcaccagat gagcatgaaa atacctatct
     7321 aaatccgtca ttgcgaggag cagagcgaag tggcaatctc atttagcaaa attttgttta
     7381 tgtaactttc tcattatttc ag
//
LOCUS       DAOSOL010000028         7394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10062_length_7394_cov_3.410683, whole genome shotgun sequence.
ACCESSION   DAOSOL010000028 DAOSOL010000000
VERSION     DAOSOL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10062_length_7394_cov_3.410683"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PK171_01340"
     CDS             <1..276
                     /locus_tag="PK171_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HOT04879.1"
                     /translation="RDFVGHGLGLSLHEEPQIPNFGSRNQGPIIKKGMVLAIEPMVNE
                     KGFQVQILDDGWTVVTRDQGLSAHYEHTVLITEDGPEILTMDRRGDE"
     gene            282..500
                     /gene="infA"
                     /locus_tag="PK171_01345"
     CDS             282..500
                     /gene="infA"
                     /locus_tag="PK171_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT04880.1"
                     /translation="MAKEEFIEVIGTVVEPLPNAMFRVELESGKVILAHISGKMRMHY
                     IRILPGDKVTIQISKYDSTKGRITYRHK"
     gene            687..800
                     /gene="rpmJ"
                     /locus_tag="PK171_01350"
     CDS             687..800
                     /gene="rpmJ"
                     /locus_tag="PK171_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOT04881.1"
                     /translation="MKVRASVKPRCEKCKAIRRNGRVFIICTNPRHKQRQG"
     gene            812..1180
                     /gene="rpsM"
                     /locus_tag="PK171_01355"
     CDS             812..1180
                     /gene="rpsM"
                     /locus_tag="PK171_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOT04882.1"
                     /translation="MARIAGVDLPNSKRIETALTYIYGIGSVLAKKIIEETQIDPSIR
                     VKDLTDEQLNKLQKAVEKYPVEGELRSQVSQNIKRLISIGAYRGIRHRRGMPVRGQRT
                     RTNARSRKGSRRTVGISRKK"
     gene            1210..1596
                     /gene="rpsK"
                     /locus_tag="PK171_01360"
     CDS             1210..1596
                     /gene="rpsK"
                     /locus_tag="PK171_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOT04883.1"
                     /translation="MVKRAKGKRKEKKVVREGIAHIKSTFNNTIVSISDRQGNVISWA
                     TSGGSGFKGTKKGTPFAAQLAAETAAKKAMDQGLREIEVLVKGPGSGRETAIRSLQAT
                     GLVIKSIKDVTPIPHNGCRPPKQRRV"
     gene            1614..2240
                     /gene="rpsD"
                     /locus_tag="PK171_01365"
     CDS             1614..2240
                     /gene="rpsD"
                     /locus_tag="PK171_01365"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOT04884.1"
                     /translation="MSRYTDAKCRLCRRQGVKLFLKGPRCMTPKCAFEKRPFAPGMSR
                     GSRRRLSDYGVQLREKQKVRFIYGISERQFNNYFEKANRIPGVTGENFLRLLEKRLDN
                     VVFRGGMADSRSDARQMVLHRHFKVNGKIVNIPSFMVSVGDKIELTDVGKQHKKIKEI
                     IDEKEQLNPPSWLEFQPEQFTFAVVREPNRDDIDYQVEESLIVEFYSK"
     gene            2256..3263
                     /locus_tag="PK171_01370"
     CDS             2256..3263
                     /locus_tag="PK171_01370"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOT04885.1"
                     /translation="MPDIKEMMKFETIELREDRITRAHYGKFSIEPLESGWGVTLGNA
                     IRRVLLSSMEGAAVTSVEIDNVIHEFSSLPGMREDITDLLLNIKGIVLSSSSPEEEVL
                     RIDVRGNEDSDLPRRVTARDIQPNPNVEIINPDHYLAELNHEGQLAMTLYVNHGKGYQ
                     ETSEKIARDYRNIQIDAIFTPVRKVNYSIVDTRVGQFTNFDKLVLEIWTNGSILPQEA
                     LKKSLDLLVGEITYIYGLVKESMSDDLATPVDVALEENEMGEDKLIEELELSVRAYNC
                     LKRARINTLRELLEIINQRPEDLKKIKNLGQKTYEEIVEKVEKLGFELNKVDVNEVNN
                     Q"
     gene            3272..3616
                     /gene="rplQ"
                     /locus_tag="PK171_01375"
     CDS             3272..3616
                     /gene="rplQ"
                     /locus_tag="PK171_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOT04886.1"
                     /translation="MKRGKLGRTTSHKEAMLSNLLVSLIKSGAVETTESKAKNLKPLF
                     ERIISQAIEGTNATTIQVMHWVDDKDAFKTLQRSLVPRLKDRKGGYCRIYKTRFRQGD
                     GAPMARVEILTE"
     gene            3688..4524
                     /locus_tag="PK171_01380"
     CDS             3688..4524
                     /locus_tag="PK171_01380"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT04887.1"
                     /translation="MIEFNGVSFWFSEEETLIEKKDEVLHNVELVIPKGEFVVLLGRN
                     GSGKSTLAKHCNGLLLPKKGTVIVDGKNTNQSEYLWDIRQKVGLVFQNPDNQIIATTV
                     EEDVAFGPENLGVPQDEIRKRIDQALNIVGMNDFRKKEPHLLSGGQKQRVAIAGVLAM
                     QPEYLVLDEATSMLDPEGRSEIMSLISQLRGSITIFHITHYIEEAVDADWVIIMDQGQ
                     VVAQGKPVDIFNRRGQIYEWGLELPLVSETAWILQRRFPELFPSLPLKAEELVAALCS
                     FV"
     gene            4509..5372
                     /locus_tag="PK171_01385"
     CDS             4509..5372
                     /locus_tag="PK171_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT04888.1"
                     /translation="MFIRLENVYYSYFQHTPFEVQALKNVTLTIDRGESVGIIGRTGC
                     GKSTLIQHFNALLFPTQGFVMIDGDKIQKKGSQLRGIRKKIGLVFQYPEHQIFEETVF
                     DEVAFGPRNNGVGGEELEETVKWALESVGLDYQELHKKNPFELSGGQMRRVAIASILS
                     MRPEALILDEPTAGLDPEGKRLILRKLKDLREENQTTLVFVSHSMDDLAMIAERIIVM
                     DQGTIGLDGSVRHIFSQGKILDQYGIKPPTTARIAELLRKKGFDIEEGILTAQELSEQ
                     IEEKILKGGSH"
     gene            5374..6180
                     /gene="cbiQ"
                     /locus_tag="PK171_01390"
     CDS             5374..6180
                     /gene="cbiQ"
                     /locus_tag="PK171_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOT04889.1"
                     /translation="MLGKSIAIGQFIPIESPVHRLDPRVKILVSAILIVALFIVKEWL
                     SFLFIGCFIILLTITARIPISYIGKSLKPILFLLAFTLVLQVFFTPGDYIWEYGFLHV
                     SREGLIRGLFIIVRLCLLILCTALLTLTTSPVELTDGLESLMSPFKKWGFPAHELAMM
                     MTIAIRFIPTLMEEADRIIKAQQARGADFESGSLIQRAKNLIPLLVPLFINSFKRADD
                     LAIAMESRCYRGGIGRSRLRVLKLRMADILFFSITIIFITVVILLSWFIW"
     gene            6199..7062
                     /gene="truA"
                     /locus_tag="PK171_01395"
     CDS             6199..7062
                     /gene="truA"
                     /locus_tag="PK171_01395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT04890.1"
                     /translation="MQKLKNNTSKCTKSNNLVLIIEYDGTNYFGWQSQPNRRSVQSVL
                     TQALSSILNQPIVLHASGRTDAGVHALGQVANCRVELTLEIPTLFRALNSYLPPDIRV
                     THLQEVSSSFNARKDATGKEYHYWMYCGYQFPVFLRHHMFYPGNFQIDWPLLKECINL
                     FVGEYDFTAFSASGSSVINMVREIHQFDVYDFKNGFLSFRIVGNGFLYKMVRILLGEI
                     WMVQLGKKKIDDLRLALSQPHRGKHRLCLPPHGLYLARVFYPDYDFWESDHLSPSFPW
                     FLSKKSSIFKL"
BASE COUNT         2298 a         1278 c         1658 g         2160 t
ORIGIN      
        1 cgggattttg ttggccatgg attaggtctc tcgcttcatg aagaacctca aattccaaac
       61 ttcggatcgc gaaatcaagg tccaatcata aaaaaaggaa tggttttagc tattgaaccc
      121 atggtcaacg aaaaaggttt tcaagtacaa atattagacg acggttggac agttgtcact
      181 cgcgatcaag ggctttcagc tcattatgaa catacggttc taataaccga agatggaccg
      241 gagatattga ccatggatag gagaggggat gaatagaata catggccaaa gaagaattta
      301 ttgaagtgat tggtacagta gtcgaaccct tacccaacgc tatgttcagg gtggagttgg
      361 aaagtggaaa agttatatta gcccatattt caggaaaaat gagaatgcac tatattcgaa
      421 tcttaccggg agataaggtc acaattcaaa tttccaagta cgattcaacc aagggacgaa
      481 ttacctatcg ccataaataa ccattaatat attttaaatg atgttaaatc atgaatcaga
      541 aataacaaga gacgaggtct tcgtaccccg aaaagactag ggttcaagat gacaaagcag
      601 gtttaaaatg ccatagttcg tcactgatcc ccaagaaatg ggagaattgt gtttctgtct
      661 tgaattttcg atagggaggt attaaaatga aagtccgcgc atcggtcaaa ccgaggtgtg
      721 aaaagtgtaa agctattcgt cgtaatggga gagtatttat tatttgtacc aacccgagac
      781 ataaacaaag gcaaggttag gaggttattc catggctcga attgctggag tcgatttacc
      841 gaacagcaaa aggatagaaa cagcgttaac gtatatttat ggcattgggt cagtgcttgc
      901 gaaaaaaatt attgaagaaa cccagattga tccgagtatt cgggtgaaag acttaaccga
      961 tgaacaattg aataaactgc aaaaagcagt tgagaaatac ccggttgagg gtgaattgcg
     1021 ttctcaggta tcccaaaata taaaacggtt aatttctatt ggtgcttatc gagggataag
     1081 acatcggcgg ggtatgccag ttcgtggtca aagaacgaga acgaatgctc gttctcgtaa
     1141 gggatcaaga cgaacagttg ggataagccg aaagaagtaa cttataatca ataaggaggc
     1201 atcgacactt tggttaaaag agcaaaaggg aaacgtaaag aaaaaaaggt ggttcgtgaa
     1261 ggaatcgccc atatcaaatc gacatttaat aatacaatag tttcaattag cgatagacag
     1321 ggaaatgtca tctcttgggc aacatcagga ggaagtggtt ttaaggggac caaaaaggga
     1381 actccctttg cagctcaatt agctgctgaa acagctgcaa aaaaagccat ggatcaaggc
     1441 ttaagagaaa tagaggtttt ggtgaaggga cctggatcag ggcgtgaaac agctattcgt
     1501 tcattgcaag cgactggttt ggtcataaaa tcaattaaag atgtgactcc aattcctcac
     1561 aatggttgcc gaccacccaa gcaaagaagg gtttaagagg aggattaatt catatgtcca
     1621 gatataccga tgcaaaatgc agattatgcc ggcgacaggg agtgaagtta tttttaaagg
     1681 ggccccgttg catgactcct aagtgtgctt ttgaaaaaag gccctttgca ccaggaatga
     1741 gtcgaggctc acgaagacgt ctctctgatt atggtgtaca attaagagag aagcaaaaag
     1801 ttcgttttat ttatggaatt tcagaacgac aatttaataa ttattttgaa aaggcgaatc
     1861 gtattccagg agttaccggt gaaaacttct tgcgtctatt agagaagcgt ttggataacg
     1921 ttgttttccg aggaggaatg gctgactcaa gaagtgatgc tcggcaaatg gtgctccatc
     1981 gccattttaa agtgaatggg aaaattgtca atatcccgtc ttttatggtt tcagtgggag
     2041 ataagattga attaactgat gttggtaaac agcataaaaa gatcaaagag attattgatg
     2101 aaaaggaaca actcaatcca cctagctggt tagaatttca accagaacaa ttcactttcg
     2161 cagtagtccg agaaccgaat cgggatgata ttgattatca ggtagaggaa tctttgatcg
     2221 ttgagtttta ctccaagtaa atttgatgtg taggtatgcc cgatattaag gaaatgatga
     2281 aatttgaaac aattgagctt cgtgaggatc ggattacccg agctcattat ggaaagtttt
     2341 ccatagaacc attagaatca ggatggggtg tgaccctggg gaatgccatt cggcgtgtcc
     2401 ttctctcttc tatggaaggg gcagcggtta cctcggtaga gatcgataat gttattcacg
     2461 agttttcttc tcttccggga atgagggagg atattaccga tcttctcctc aacattaaag
     2521 gcatagtgct ttcttcatca tcaccagaag aagaagtgct aagaattgat gtaaggggaa
     2581 atgaggattc cgaccttcct cggcgagtga cagctcggga cattcaaccc aatccgaatg
     2641 tcgagattat taatccagat cattatttag ccgagttgaa tcacgaagga caactggcga
     2701 tgactcttta tgtcaatcac ggtaaggggt atcaggagac ttcggaaaag attgcacgtg
     2761 attatcggaa tattcaaatt gatgcaattt ttaccccggt tagaaaagta aattatagta
     2821 tagttgatac acgggttggt cagttcacca actttgataa gcttgttctg gaaatttgga
     2881 ccaacggatc tatccttcct caagaagctt taaaaaaatc tcttgatttg ttggttggtg
     2941 aaattactta tatttatggc ttggttaagg aaagtatgtc agatgattta gctactccag
     3001 ttgatgtcgc tttagaggag aatgaaatgg gagaagacaa gctaattgaa gagttagaat
     3061 tatcggttcg agcttataat tgtttaaaac gtgctcgaat taatactctt cgtgaacttt
     3121 tggaaatcat taaccaacgc ccagaagatt taaagaaaat caaaaactta ggtcaaaaaa
     3181 cctatgaaga gattgttgag aaggttgaaa aacttggttt cgaactcaat aaagtagatg
     3241 taaatgaggt gaacaaccaa tgaggcatag aatgaaaaga gggaagttgg gtcgtaccac
     3301 ctcccataaa gaggctatgt tatcgaatct tttagtttcc ttgattaaat caggagcagt
     3361 ggaaacaact gagtcgaaag caaaaaacct caaaccgctt tttgaaagaa ttatatctca
     3421 agcaattgaa ggaaccaatg caacaactat tcaagttatg cactgggttg atgataagga
     3481 tgcttttaag acccttcaaa gaagtttagt accacgtttg aaagatagaa aaggaggata
     3541 ctgtcgaatt tataagacca gattccgcca aggagacggg gctccaatgg cacgggttga
     3601 aatacttact gaataagacg gtgagctgga tagtaataac cctgccagcc aaaaggctgg
     3661 ctttttttat ggaaaggacc tctgaaggtg attgaattta atggtgtatc cttctggttt
     3721 tctgaagagg aaaccttaat agaaaaaaag gatgaagttc ttcacaatgt cgagctcgtt
     3781 attcccaagg gtgagtttgt tgtattattg ggaaggaatg gttcgggaaa atccaccttg
     3841 gccaagcact gtaatggatt attgcttcct aaaaaaggaa cggttattgt agatgggaaa
     3901 aacaccaatc aatcagagta tttatgggat attcgtcaaa aggttggatt agttttccaa
     3961 aatcctgata accagattat cgccactact gttgaagaag atgttgcctt tggaccagag
     4021 aatcttggag tacctcaaga tgaaatacga aagcggattg atcaagcatt aaatatagtt
     4081 ggaatgaacg attttcggaa aaaagaaccc catctccttt ctggtggaca aaaacagcga
     4141 gtagcaattg ccggtgtttt ggctatgcaa ccggagtatt tggtgcttga tgaggcaaca
     4201 tcaatgcttg acccggaagg cagaagcgaa attatgtcgc ttatttctca attacgagga
     4261 tcaatcacta ttttccatat tacccattat attgaagaag ctgttgatgc tgattgggtt
     4321 attataatgg atcaaggtca agttgttgct cagggaaaac cagtcgatat ttttaatcga
     4381 agaggacaaa tttatgaatg gggtttggag ctcccgttgg tatcggaaac tgcctggatc
     4441 cttcaaaggc ggtttcccga gcttttccct tcgcttcccc ttaaagcaga agaactggtg
     4501 gctgccttgt gttcattcgt ttagaaaacg tctattacag ttatttccag catacccctt
     4561 ttgaagttca agcccttaag aatgtcactc ttactattga taggggagag tcagtaggta
     4621 ttattggaag gactggctgt ggaaagtcca ctctcattca gcattttaac gctcttcttt
     4681 ttcccactca aggattcgtt atgattgacg gtgataaaat ccaaaaaaaa ggttcccagc
     4741 ttagggggat tagaaaaaaa atcggcttag tatttcaata tcctgaacat caaatttttg
     4801 aagaaacggt ttttgacgag gttgctttcg gacctcgaaa taacggtgtg ggtggtgaag
     4861 agttagaaga aacagtaaaa tgggctttgg aaagtgttgg tttggattac caggaactgc
     4921 ataaaaagaa tccttttgaa ttaagtggag gccagatgag acgagttgcc attgccagca
     4981 ttttgtcaat gcgacccgaa gccttaattt tagatgagcc gacggctgga cttgatccgg
     5041 aagggaaaag attaattctg aggaaactga aagatcttcg agaggaaaac caaacaaccc
     5101 tggtattcgt ttcacatagc atggatgact tggccatgat agcggaaaga ataattgtga
     5161 tggaccaggg aactattgga ttagatggct cagtacgtca tatattttct caaggaaaaa
     5221 ttctggatca atatggaata aaaccaccta ctactgctcg aatagctgaa ttgctgcgga
     5281 aaaaaggatt tgatattgaa gaggggattt taactgctca ggaattatct gagcaaattg
     5341 aagaaaagat attaaaagga ggaagccatt aacatgctgg ggaagagcat agcgattgga
     5401 caatttatcc caattgaatc gcccgtccac cggttggatc ctcgagtaaa aatcctggtt
     5461 tctgctattc ttattgttgc tttattcatt gtcaaagaat ggctatcctt tctttttatt
     5521 ggatgtttta tcatcttgct aaccattact gcccggatac caatttctta tatcggaaaa
     5581 agtttaaaac caattctttt tctcttagct tttactctgg ttttgcaggt tttttttacc
     5641 cctggagatt atatttggga gtatggtttt cttcatgttt ccagagaggg gctcatccgc
     5701 gggttgttta tcattgttcg gctttgtctt ttaattttat gtaccgctct tttaaccctt
     5761 accacttcgc cagttgaatt gaccgatggg ttggaatcac tcatgagtcc tttcaaaaaa
     5821 tggggtttcc cggcccatga attagcgatg atgatgacca ttgctatccg ttttataccg
     5881 accttgatgg aagaagctga ccgaatcatc aaagcccaac aagccagagg agctgatttt
     5941 gaatcgggaa gcttgattca acgagctaaa aatcttattc cactgttagt tcctttattt
     6001 attaattctt ttaagcgagc cgatgacttg gcgattgcta tggagtctcg atgttatcgg
     6061 ggtggtatag gtcggagtcg gcttcgagtc cttaagttaa ggatggctga tattcttttt
     6121 tttagcatta caataatttt tataaccgtg gttattttat tgtcttggtt tatttggtaa
     6181 aggatactta agatatttat gcaaaaattg aaaaataata catcgaaatg cactaaaagt
     6241 aataatttag tactgataat cgaatatgat ggaaccaatt attttggttg gcaaagtcag
     6301 ccaaatcgtc gtagcgtcca atcggttctc actcaagctc tctcatcaat tttaaatcag
     6361 ccgatagttc ttcatgcctc tggtcgaact gatgccggag ttcatgcctt aggacaggta
     6421 gcgaattgcc gtgtcgaatt aaccctggaa ataccaactc tttttcgagc gttaaattcc
     6481 taccttcctc ctgatatcag agtgacacac cttcaggagg tatcttcatc ctttaatgct
     6541 cgcaaagacg ctaccggtaa agaatatcat tattggatgt attgtggata ccaatttcca
     6601 gtgtttttgc gtcaccatat gttttatcca gggaatttcc agattgattg gccactttta
     6661 aaagaatgca tcaatctatt tgtaggagaa tatgatttta ctgcctttag tgcttctggg
     6721 agctcagtaa ttaatatggt tcgagaaatt catcaatttg atgtttatga ttttaagaat
     6781 ggttttttgt ctttccggat cgttggaaat ggatttttgt ataaaatggt tcgaattctt
     6841 ttgggagaaa tctggatggt tcagttagga aaaaaaaaga ttgatgacct tcggttggct
     6901 ttatcccaac ctcatcgagg gaagcatcga ttatgccttc ctcctcatgg tttgtatctc
     6961 gcccgggttt tttatccaga ctatgatttt tgggaaagcg atcacctttc gccatccttt
     7021 ccatggtttc tttcaaaaaa gtcttcaata tttaaattat gaagtaaaaa ccccttccat
     7081 tttttatcct gaaaacccac gagcaggata aatcaagacc ctaccaaaga atatataatg
     7141 taggggcgac aatgtattgt aaccattctt taattcatgt agcgacatgc catggcatgt
     7201 cgaatccttg gattttcgct tttatctggt gcgaccatgg gataagactc tattaaaaat
     7261 atttcaaaaa aaaccctgag gtatggttga caattccata taggctgtgt taaattaact
     7321 cttgccctcg ggttaaggta aaagatccga aggctagagt accttgaaaa gtaaatattc
     7381 cgcctacttc caaa
//
LOCUS       DAOSOL010000029         7331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10163_length_7331_cov_3.985981, whole genome shotgun sequence.
ACCESSION   DAOSOL010000029 DAOSOL010000000
VERSION     DAOSOL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10163_length_7331_cov_3.985981"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PK171_01400"
     CDS             complement(<1..465)
                     /locus_tag="PK171_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT04891.1"
                     /translation="MENLRSKIEESAHFIQERIDFSPEIGIILGTGLGALADDIEIDR
                     VISYDEIPWFPVSTVESHHGNLIIGELSGKKIVAMQGRFHYYEGYSMKQVTFPVRVMK
                     ALGIKVLIVSNAAGGMNRNMQRSDLMIICDHINLLGDNPLRGENDPELGPRFL"
     gene            complement(553..1455)
                     /gene="xerD"
                     /locus_tag="PK171_01405"
     CDS             complement(553..1455)
                     /gene="xerD"
                     /locus_tag="PK171_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOT04892.1"
                     /translation="MLSNDLRNILEAYLQFLYWEKRLSDNTIQSYEQDLEQFVVFLEK
                     KGMVSFSRLDQDIVEKFLRYEAKREIAPASLSRKISALRTFFQYLIREKMSPENCAKL
                     IDSPRFQRKIPDVLDEVEIESLLNAPDLTKPIGIRDRAILELLYASGLRVSELTSLEF
                     SHLDLENSMLRLWGKGFKERMVPFGEEASEALKRYFQQAREQILKEKRSRSIFVGASG
                     QPLTRQNIWVMIKKYSRKAGIEKTVTPHTLRHTFATHLLENGADLRVVQEFLGHSDIV
                     TTQIYTHISKKILREAYARSFPRK"
     gene            complement(1424..2191)
                     /gene="lgt"
                     /locus_tag="PK171_01410"
     CDS             complement(1424..2191)
                     /gene="lgt"
                     /locus_tag="PK171_01410"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOT04893.1"
                     /translation="MHSVIFRIGQFPVYAYGFFLGVAFLVGIYYASRRAPGYGVAPDA
                     VVEVSVLCIFGAIIGSRLVFVLLNWDIYRDNLIHIFLIREGGLTFYGGIFGAIFLAIP
                     YIIYKKYSLPALFDICTPAIALGYSIARIGCFLNGCCYGRVCSYTHFPLGVHFPALEG
                     WRYPTQIYAVGYSLLILFFLLKLEKKKVFRGELFFDYLWMYGIARFFIEYFREEPFSV
                     WGIMTAAQFACILIIIIALILREILRRHAIQRSQEHS"
     gene            complement(2191..3651)
                     /locus_tag="PK171_01415"
     CDS             complement(2191..3651)
                     /locus_tag="PK171_01415"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOT04894.1"
                     /translation="MNNFLINEKKVWTFSLTLSVIINLVILAAISIYWLSIKYEPPLQ
                     DEPMVIQVLEIPSTRKPVTVAPVEEANIAELNPLKDIQNQDVNNEPQIQSEIATEVQQ
                     KKEQVTIPKPPIDLPDSEMISESGSPSGAKLVNPGENIDVPEDLKWQGVEAENTLKAQ
                     FERSGQSTRLARLAEEGAERIESTVGETLATGELAIPTGQVEKKSPFSKRPISVIIEN
                     APAARPQAGLSKADIVYEIMAEGGITRFLAIYNEGVADNVGPVRSARPYFVMKAAEHN
                     AIFAHAGGSVEAYVYMKEMNIDTIDEFKFFQAFWRSKDRKAPHNLYTSIANLRNQAQR
                     LGYNKPVKGGGGFQIRTPQEALGTEDASQVEILYAGDYRVKYTWDPAQRIYRRYINGN
                     PHVDSLNGQQIKTPNLIIQITEQKVKDEEGRIEITFVGKGTGWILLDGKAAAIRWEKN
                     SIGEKTNFYYADGRDLKINPGALWIEVVSTQNKVAM"
     gene            complement(3654..4070)
                     /locus_tag="PK171_01420"
     CDS             complement(3654..4070)
                     /locus_tag="PK171_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOT04895.1"
                     /translation="MFRFRQKKPRRQPEINLTPMIDVVLQLIIFFIVTTTFISIESGA
                     KVNLPSADFSKIEEVKTITVTITENNMLYVNGALVDANELPSAVVVALRNEPEATVIV
                     EADKQVLHGKVVSVMDILKKAGAEKIAIATQPTKEE"
     gene            complement(4030..4746)
                     /locus_tag="PK171_01425"
     CDS             complement(4030..4746)
                     /locus_tag="PK171_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOT04896.1"
                     /translation="MQIFTVIGKGGLIMYPIVASSIIFLAVFIERWYVLRHSKERVKR
                     YLRALRKAINQRDLRNIVDISQKNPGPASRIIMAGIKKYLNGVPREAKEAMEAVAMQE
                     FPYFEKRLGVLSTIASIAPLLGLLGTVTGMIRTFNVIASIGVGKPTEMAGGISEALIT
                     TAAGLSIAIPTVIGHYYLSQIAESIMNDIEKASSEVLDIIEELRINNGNEGVIFEETK
                     SPEGVEENVPISTKETEAPA"
     gene            complement(4800..>7331)
                     /locus_tag="PK171_01430"
     CDS             complement(4800..>7331)
                     /locus_tag="PK171_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT04897.1"
                     /translation="TYYANGDLKTAEGYFRNATQLNPDMKEAQYWLGRTLYDLGKLAD
                     AEVTWRKVIEIDPFDSQAKRFLERTIQEKQYGRNALSQFRQGFDLYQEAKYAEAIPYF
                     ERAVQESPRFPDAHEYLARSYYLLGQKDKYLKEREKSLNLIEQPADKAWQYYLVGYEL
                     FSWNEKEKAIGYLQKAVAVDDRLSEAHLLLGEIYGSMNQWKQAAYHYTQASSVPENNT
                     EEDQSSVLWGASVSYVQLGEWDKALEFLNELVKKYPYADFIEEAESLRIEAMVKMGQY
                     RDARVSVQQFQLRFPSGRFRERVQFYYAFSFYQEKQWKEAVQALESFHKSYPQSQYRK
                     ETLEALGYSYRNLGQEEKARSYFSQVEGQENAFLVADTFYREKKWQQALSSFLEYLQT
                     TPQGQYVQEARLKLASCYLETNQVELAEKLVNEIAGSLDAKFQLDFLRLTIKLAYKKG
                     NWQKVVEGVGQLEKQSGTLDLEYLYLLAWSQVKLGKESEARELLEKADLNPDEILSDP
                     EIEKISDIMDTLQSGDYPSAILQLKELITEGVNSENSAIVHFLYGKALYFNGNYDEAI
                     QYLKEAVAAGNKDYLEEAYFYLGDIAYKEENWSEAAQWYQRLTTQDNLDLLWRLATSL
                     EKLGKKDQALDIYKKLKGDNAYSERAGIIVLETLYDQKKYQDFLKEATEYLENHPDSV
                     QNEEILYMTAWSAYYLGNAPEALERISLYQDKYPQGQYFDELESLAVDLMIVQKNYQD
                     VLPKLFTLENKLKGEKLEYTWYRIGSVYLKLEGYDRAAFYFEKLLGNVNGKYYTRGGY
                     LMGVCLEYLEQPETALKFYHQIVESGLKDEWVENSQKRISLLTED"
BASE COUNT         2128 a         1629 c         1297 g         2277 t
ORIGIN      
        1 caaaaatcgt ggtcccaact ccgggtcatt ttcaccacga agtggattgt cacctaaaag
       61 gttgatgtga tcacaaataa tcatcaagtc actgcgctgc atattccgat tcattccacc
      121 ggcggcatta ctgacaatta ataccttaat ccccagtgct ttcataaccc gaaccgggaa
      181 ggtaacctgt ttcatcgagt atccttcata atagtgaaat cttccctgca tagcaacgat
      241 cttttttcct gataattcac cgataattaa atttccgtga tggctttcaa ctgtggaaac
      301 tggaaaccag ggaatttcat cataggaaat aactcgatca atctcgatat catcggcaag
      361 agctcctaaa ccagttccca agataatgcc tatttcagga gaaaaatcaa ttctttcttg
      421 aatgaaatga gcactttctt caatttttga acgtaaattc tccataaaaa caccccttca
      481 aaattataat tgtcacactt ggtaaaaagc aaatattatt ggtaatcctt gggttttaaa
      541 aaaacttgat tattattttc tgggaaatga acgggcatat gcttcacgaa ggattttttt
      601 actaatatga gtatagattt gagtagtcac gatatcggaa tgtcccaaaa actcctgcac
      661 aactctcaaa tcagctccgt tttccaacaa atgggtggca aaggtatgtc gaagagtgtg
      721 cggtgttaca gtcttctcaa ttccagcttt tcgggagtat ttcttaatca ttacccaaat
      781 gttttgccgg gttaaaggtt gaccggaggc tccgacaaaa atcgaccgac ttcgtttttc
      841 ctttaatatt tgttctcttg cctgctgaaa atatcgcttc aacgcttcgc tggcttcctc
      901 gccaaaaggt accatcctct ccttaaagcc tttgccccaa agccgtaaca tagaattttc
      961 caaatcaagg tgtgaaaatt ccagagaggt taattcgctt accctcaatc cactggcgta
     1021 taataattct aaaatagctc ggtctcgaat tccaatcggt ttagtcaaat ccggagcgtt
     1081 caataaggat tcgatttcaa cttcatctaa aacatcgggt atttttctct gaaaacgagg
     1141 agaatcgata agcttggcac aattttcagg actcattttc tcacggatca aatattggaa
     1201 aaaggtacgg agggctgaaa ttttcctgct aagtgaagca ggagctattt cgcgcttggc
     1261 ttcatatcgt aggaattttt caactatatc ctgatcgagt cgggaaaaag ataccatacc
     1321 ctttttttcc aaaaaaacaa cgaattgctc caaatcctgc tcataggatt gaatcgtgtt
     1381 atcagaaagt cttttttccc aatacaaaaa ctggagatac gcttcaagaa tgttcctgag
     1441 atcgttggat agcatgtctt ctcaatatct ctcgtagaat aagagcaatt ataattatta
     1501 atatacaagc aaattgagct gcagtcatga ttccccaaac cgaaaagggc tcttcacgaa
     1561 aatactcaat gaaaaaacga gcgatcccgt acatccacag ataatcaaaa aagagctctc
     1621 cacgaaaaac cttttttttc tccagtttca gtaaaaaaaa gaggatcaga agagaatacc
     1681 ccaccgcata gatttgagtt ggatatctcc acccctctaa agcagggaaa tgaaccccaa
     1741 gaggaaaatg agtataggaa caaactcgac cgtaacaaca accgttgaga aaacaaccaa
     1801 ttcgagcaat ggaatagccc agagctatag ccggagtgca aatatcaaat aaagccggta
     1861 aagaatattt tttataaata atataaggaa ttgctaaaaa aattgctcca aaaataccac
     1921 catagaaagt cagaccacct tcacggatta aaaagatatg aatgaggtta tcacggtaaa
     1981 tatcccagtt gaggagaaca aaaactaagc gagacccaat gatggccccg aaaatacaga
     2041 gcacactgac ttcaaccact gcatcgggag caactccata accaggagct cgccttgagg
     2101 cataataaat acccaccaaa aaagccacgc ctaaaaaaaa gccataggca taaacaggaa
     2161 attgaccaat tctaaatata acactgtgca ttacatcgcc accttattct gggtactgac
     2221 cacttctatc catagcgccc cggggttaat tttcaagtcc cttccatcag cataataaaa
     2281 gttggttttt tctcctatag aattcttttc ccagcgtatt gcagctgctt tcccatctaa
     2341 taaaatccat ccagtacctt tcccaacaaa agttatttct attcgacctt cttcatcttt
     2401 cactttctgc tcggtaattt gaatgattag attgggagtt ttgatttgtt gaccgttcaa
     2461 agaatcgaca tgggggtttc cattgatata tcttcgatag attctttggg caggatccca
     2521 ggtatatttt actcgataat caccagcata caagatttcc acctgagaag catcttcagt
     2581 tcctaaggct tcttggggtg ttcgtatttg gaatccccct cctcctttca ccggtttatt
     2641 ataacctaac cgctgggctt gatttctgag gttagcaatc gaggtgtaga gattgtgagg
     2701 ggcttttcga tctttacttc tccaaaaagc ttgaaagaat ttaaattcat cgatagtatc
     2761 aatgttcatt tccttcatat agacatacgc ttcaacactg ccgccggcat gtgcaaatat
     2821 ggcattatgt tcagcagctt tcatcacaaa atagggacga gcgcttctaa ccggaccaac
     2881 attatccgct acaccttcgt tatatattgc caagaaccga gtgattccac cttcagccat
     2941 aatttcatag acgatatctg ccttactgag cccggcttga ggtcgtgccg cgggggcatt
     3001 ttcaattatg actgaaatcg gccgtttaga aaagggagat tttttttcaa cctgaccagt
     3061 tggtatagcc agctcaccgg tagccaatgt ttcaccaacc gtactttcta tcctttctgc
     3121 tccttcctca gccaaacggg ctaaacgggt tgattggcca gatctttcaa actgggcttt
     3181 cagggtgttt tcggcttcaa ctccctgcca ttttaaatct tctggaacat cgatattttc
     3241 tcctggattc actaattttg ctcccgaagg tgatcccgat tcagaaatca tttccgagtc
     3301 gggaaggtca attggtggtt ttgggatggt aacctgttct tttttttgct gaacttcagt
     3361 cgctatctcg ctttgtatct gaggctcatt attaacatct tgattttgaa tatcctttaa
     3421 aggattcagt tcggcaatat tggcttcttc aactggagca actgttactg gcttacgtgt
     3481 ggatggtatt tctaaaacct gaatcaccat aggttcatct tgaagaggag gttcgtattt
     3541 tatcgataac caatagatag atattgcagc caaaataacc agattaataa taactgataa
     3601 ggttagggaa aaagtccaaa cctttttttc attaattaaa aaattattca taattattct
     3661 tctttggtcg gttgagtggc gatggctatt ttctcagctc cggccttttt aagaatatcc
     3721 atgaccgaaa caacttttcc gtgcaaaacc tgcttatctg cctctacaat gactgtcgcc
     3781 tctggttcat tacgcaatgc caccacaacc gccgagggaa gctcattggc atcaacgaga
     3841 gcaccattga catagagcat gttgttttcg gttatggtta cagtgatagt cttgacttcc
     3901 tcaattttgg aaaaatctgc cgagggaaga ttcaccttgg ccccactttc aatgctgatg
     3961 aacgtggtgg ttacaataaa aaatattatt aattggagca ccacatcgat catcggagtg
     4021 aggttaattt caggctggcg cctcggtttc ttttgtcgaa atcggaacat tctcctcaac
     4081 tccttctggg ctcttggttt cctcgaagat tactccttca tttccattat taattctaag
     4141 ctcttcaata atatctaaaa cctctgaact ggctttttca atatcgttca taatgctttc
     4201 ggcaatctgg gaaaggtagt agtgaccaat aaccgtagga atagcgattg ataatccagc
     4261 agcagttgtt atcaatgctt cagagattcc tcctgccatt tcggttggtt ttcccactcc
     4321 aatcgaggca ataacattaa aagttcggat cattccagta accgttccaa gcaatcccaa
     4381 taatggggca atactcgcaa tagtcgatag cactcccaag cgcttttcaa aataaggaaa
     4441 ctcctgcatg gctacagctt ccattgcttc ttttgcttcc cgaggaacac cattcagata
     4501 tttttttata cctgccatga taatccgcga tgctggacca ggatttttct ggctaatgtc
     4561 aacaatgttc cgcaagtctc tttgattaat agcttttctc aacgccctta aatatctctt
     4621 tactctttct tttgaatgcc gtaaaacata ccacctctcg ataaaaactg ctaagaaaat
     4681 aatcgatgag gccacgatcg gatacatgat taatccgccc ttgccaatta cagtaaatat
     4741 ctgcatcaat gaaacctcct gcattgtgga gaaagaatca gcataagtgt atcctctgtt
     4801 taatcctcag tcaatagact gattcgtttc tgtgaatttt caacccattc gtcttttaag
     4861 ccgctttcaa caatctgatg ataaaacttc aaggcggtct ccggttgttc taagtattca
     4921 agacaaacac ccattaaata tccaccccga gtatagtatt tcccattcac atttcctaat
     4981 aatttttcaa aatagaaagc cgctcgatca tatccttcta attttaaata gacacttcca
     5041 atacgatacc aagtatactc caatttttct cctttgagtt tattttctaa agtaaataat
     5101 ttgggaagga catcttgata attcttttgt acaatcataa gatcgactgc taatgactcc
     5161 aattcatcga aatattgccc ttgagggtat ttatcctgat aaagagatat ccgttccaat
     5221 gcttcaggag catttcctaa ataataagcc gaccaagcgg tcatatataa tatttcttcg
     5281 ttttgaaccg aatcgggatg gttctctaaa tattctgtgg cttcctttaa aaagtcctga
     5341 tattttttct gatcgtataa agtttccaaa acaataatgc cagctcgttc agaataggca
     5401 ttgtcgcctt ttaatttttt ataaatatca agagcttgat cttttttccc caatttttct
     5461 aacgaagtcg ccagtcgcca gagaaggtca agattatcct gggtggtcaa cctttggtac
     5521 cattgagctg cttctgacca attctcttcc ttataggcaa tatcacctaa ataaaagtaa
     5581 gcctcttcca gatagtcttt gtttccggca gcaactgctt cttttaagta ttggatagcc
     5641 tcatcataat ttccattaaa atataaagct ttcccatata aaaaatgaac tatagcgcta
     5701 ttctcagaat ttactccttc ggtgatgagt tcttttagtt gtaatatcgc cgaaggataa
     5761 tcacccgatt gaagagtatc cataatatcg cttatttttt caatttccgg atcagaaagg
     5821 atctcatcgg gattcaaatc cgctttttcc aacaactctc tcgcttctga ttcttttcct
     5881 aattttacct gggaccaagc taaaaggtat aaatattcca ggtcgagcgt cccggactgc
     5941 ttttctaatt gaccaacacc ctcaaccact ttctgccaat tccctttttt ataagccagc
     6001 tttatagtga ggcgtaggaa atccagttga aacttagcat ctaacgaacc agctatctcg
     6061 ttaaccaatt tttcagccaa ttcaacctgg ttggtctcta aatagcatga ggccagtttt
     6121 aatctggctt cctggacata ttgaccttgg ggagtagttt gcaggtattc taaaaatgat
     6181 gacagagctt gctgccattt tttttctcga tagaaagtat cagctaccaa aaaggcattt
     6241 tcctgacctt cgacttgaga aaaataactc cgagcttttt cttcttgtcc caagtttcga
     6301 taggaataac ccaacgcttc taaggtttct ttccgatatt gactttgagg ataactttta
     6361 tggaatgatt ctaaggcttg aacagcttct ttccactgct tttcttgata aaagctaaat
     6421 gcataataaa attgcactct ttctcgaaag cgtcccgatg gaaaacgtaa ctgaaattgt
     6481 tgcacactga cccgggcgtc gcgatattgg cccattttta ccatcgcttc aatccgtaat
     6541 gactctgctt cttcaataaa atcggcataa ggatatttct ttaccagctc attgaggaat
     6601 tcaagtgctt tgtcccattc tcccaactga acgtatgaaa cactcgctcc ccacaatacc
     6661 gaggattggt cttcttcggt attattttcg gggacagaac tggcttgggt ataatgataa
     6721 gcagcttgtt tccattgatt catgcttccg tatatttcac ccaatagaag atgagcttcc
     6781 gacaaacgat catccacggc taccgctttt tgtaagtaac caatggcctt ttctttttca
     6841 ttccaagaaa aaagttcata cccgactaaa taatattgcc aggccttatc agccggttgt
     6901 tcgatgagat tcagactttt ttctctttct tttaagtatt tatccttttg tcctaacaaa
     6961 taataggaac gggctaaata ttcatgagca tcgggaaaac gagggctttc ctgaacggct
     7021 ctttcaaaat agggaattgc ttcagcatat tttgcttctt gatagagatc aaaaccctga
     7081 cggaattggc tcagagcatt tctcccatat tgtttttctt gaatagttct ttctaagaag
     7141 cgttttgcct gactgtcaaa gggatctatt tcaataacct tcctccaggt cacttcggcg
     7201 tcggccaatt tacccagatc gtaaagggtt ctccccaacc aatattgggc ttctttcatg
     7261 tccggattta attgggtggc gttgcgaaag tatccttcag ctgttttgag atcgccattg
     7321 gcataataag t
//
LOCUS       DAOSOL010000030         7276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10271_length_7276_cov_3.592577, whole genome shotgun sequence.
ACCESSION   DAOSOL010000030 DAOSOL010000000
VERSION     DAOSOL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10271_length_7276_cov_3.592577"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1513
                     /locus_tag="PK171_01435"
     CDS             44..1513
                     /locus_tag="PK171_01435"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOT04898.1"
                     /translation="MCHKKYKGELMQLPDLKVLSESEILAIHENSLVILESVGVRVES
                     KKILDVLEKKGCLVDFEIERVKFPPSVVKECLSSLPKQIPIFQRDGDLAFILGDGGQY
                     CVSGHNAVFVLINEKGERRNSTVKDVENFAIISDKLSEVDMVGVPVMPQDVTPQTTLL
                     YAIQAILENSKKPIFFSSESEIINQAVIDMGKVITGKEKLKGCSPMISQLSTTSPLYW
                     ERGAVEALYIVAQEGIPLDLLPQPIAGVTAPYTLAGLLTVHNTEVLSGIIISQLIHPG
                     TPVIYGAAWTTYEMKQANVLIGRPESSLLRIAGAQMAHYYNIPSHTTAPDNDANSHDE
                     QAAWEKMLSTLAGIIGANDMIVNLGMFGTGMTISLEQLVMDNEMCRIAKRFRQGIEVN
                     KDTLALDSILQVGPRGEYLTSEHTLHHLRSGEHVPLDVSNGTNFEVWKQKGSKNTTEK
                     SSDLVSSILLGGCQNPLSQDKQKAIQSIINQYEKKLGLR"
     gene            1561..2862
                     /locus_tag="PK171_01440"
     CDS             1561..2862
                     /locus_tag="PK171_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04899.1"
                     /translation="MTPRIGLIGIAGPLEVGFSETPYILQRMKNQLENIAPVITNLDI
                     IYDLQTVQKAVNFFANHDFDLLCVGVGTWSEDHHLLDFLEYYNRPVVLWALPAVETGS
                     LCGVQQICCVLKELERPYFFVYGEPEDVKVHQKIREIAMAVTLGNHLRHSRIGVIGGR
                     VKGMTEIAYDELEIKARTGVRIVNLDEDELIESVKNTDQLAASKLWTAIGEKVGKITV
                     NNQTGIEALSYYFGLKKLIEEYDLDGICIKCYPRYMGKICLAYSLLAEEEIVGGCEGD
                     VNNTVTMKILFNLTGKPVHNTDFLYPDPISQTVLFSHCGSGGFSIANDPAQIELSPVR
                     LLNQGVCALFPARIGVVTLVNLVGRRGTFRMSALTGEAIECGMEFPGNPLKVRFQRSL
                     EDLNEEIASQGIGHHWMAGYGDVRKELEFFCSLNKIQYYSL"
     gene            2941..4008
                     /locus_tag="PK171_01445"
     CDS             2941..4008
                     /locus_tag="PK171_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT04900.1"
                     /translation="MFKQKFFSLFLAIVFVAVSLLFSLAYAQETLNILCFQGYTEDVW
                     VKEFERLTGVKVNATYSGMVEEMFTKAAAGGGQYDLVTIDCGSVQRYYENNLLQPIDL
                     SKISNWEKVSQKFKDIDFTVFDGKPYHVSFVWGSNNVVYNKDAVGELPTTWSVLWDPK
                     YKGQVSITDEANNNVVVAAIALGFPDPYNLTEDQFKQVKEKLIEIKRNLRTLTVGFDS
                     EKSVLGSGETNLSVSGYDSGLILYLRDELKMNVGRLTPKEGIYVWADGWVILKDSKNP
                     DLAHKWIDFMLSDFAQKELAKYMGFGAVTSAAKEVIDPDIVKMCNYDDIDNVPVPVFL
                     MKSPEDFEARVNLWNEVKATQ"
     gene            4219..5355
                     /locus_tag="PK171_01450"
     CDS             4219..5355
                     /locus_tag="PK171_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT04901.1"
                     /translation="MNNHETEIQLIQITKKFGNFTAVQNVNLEIHHGEFFSLLGPSGC
                     GKTTTLRMIAGLEEPTEGQICLVGEDVTYKPAYLRNVNTVFQDYALFPHMTVEENIHF
                     PLKMKKVKYSSARTMIEEALSLVNMTGFGKRYPQQLSGGQRQRIALARSLVNRPRALL
                     LDEPLGALDFKLRIAMQKVLKDIQKNVDITFIYVTHDQTEAITMSDRICVMKDGLIHQ
                     IGTPDEIYNSPASTFVASFIGDMNFLEGQVVQKGENSITIDVDGKPILSTRFKGSFSK
                     GDSVVACIRPEKICLDKVCDSEFQNKFENHFQAAIHRIVFRGTDYEITLKFGQSEIRL
                     IESASDFNDKKIGDQLFIGFQKDDVITYLKECKKVCDYETNLDN"
     gene            5333..6226
                     /locus_tag="PK171_01455"
     CDS             5333..6226
                     /locus_tag="PK171_01455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT04902.1"
                     /translation="MKPTLTTKLNARPRGQSSFFSKIWIGVPITTWLFLLIIIPICIM
                     LVTSFYIKEGSVVVKKISLVNYLEFFINPIYLPIFFRTLLLALLVSLTSILLGYPLAY
                     LVSRKVKRFRNQLYMLVIVPLWVSYLVRIVAWRTILGRVGVLNTILLSLKIIDEPLSI
                     FIYSPFAVYVALIYIALPYVFISIFNSLEKIPKNLLDASADLGANSFNTFLHVILPLS
                     MPGVISGFTLAFVIALGDYIIPQQLGGLNGMMFGNLIVTQFGYAYNWPLGAALGFIMF
                     TVAVVILLVSQKFGSSEGFLE"
     gene            6226..7041
                     /locus_tag="PK171_01460"
     CDS             6226..7041
                     /locus_tag="PK171_01460"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT04903.1"
                     /translation="MKKTSNLKSKNKSFPFLSIYGTLVYFYLYFPLLVVFVYSLNSSR
                     STMKFEGITFDWYMKLFQNKELLNSLFHSLQVSGLAVLFAVVMGTSGALFLNRIEFKG
                     KDFFRTLAMLPIILPGIIVGLSLLIFFLNLKLQLSMWTVILGHMSFTTPVVMFQVLAR
                     LARLSRNLENAAMDLGANPFQAFIYVTFPMIKRAVIGGALLAFTMSFDEIIITYFLTS
                     TYNTLPIYLYGMLRFGLSPEVYAISTVVLGLSILLIILMAKYTGSGEESIVVR"
     gene            complement(7047..>7276)
                     /locus_tag="PK171_01465"
     CDS             complement(7047..>7276)
                     /locus_tag="PK171_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04904.1"
                     /translation="GTYLIVKALTPQVMLIGAVTIIAILISIIILKKADQLIQPEYSI
                     TGYLQDDDCSQCPFKSKKTKEEEEEVDTQDK"
BASE COUNT         2168 a         1343 c         1431 g         2334 t
ORIGIN      
        1 aataattaaa gtaaagcata aaaaccgaag tataagaagg tcaatatgtc ataaaaaata
       61 taaaggagaa cttatgcaac tacctgattt gaaagttctt tctgaaagtg aaattctagc
      121 aattcacgaa aattccttgg ttattcttga atcggttggt gttcgtgtcg aaagtaaaaa
      181 aattcttgac gtattagaaa aaaaaggttg cctagttgac tttgaaattg aacgagtaaa
      241 attcccacct tcggttgtca aggaatgttt gtcatctcta ccaaaacaaa ttcccatttt
      301 ccaacgagat ggcgaccttg cttttatttt aggggatgga ggacagtatt gcgttagcgg
      361 gcataacgca gttttcgtcc tgataaacga aaaaggagaa cgtcggaatt caactgttaa
      421 agacgttgaa aattttgcca tcatttctga taaactttcc gaagttgata tggtgggggt
      481 tccggttatg cctcaagatg taacccccca aaccaccctc ctttatgcaa ttcaagcaat
      541 tcttgaaaat tccaaaaaac ccatcttttt ctcctctgaa agtgaaataa tcaatcaagc
      601 agttatagat atgggtaagg tcattaccgg aaaagagaaa ttgaagggtt gttctccaat
      661 gattagccaa ctctcaacca ccagtccact ctattgggaa aggggagcag tggaagctct
      721 ttacatcgtt gctcaggaag ggattcccct tgatctcctt cctcaaccaa ttgcgggagt
      781 tacagcaccg tatactttag ccggtttatt gacagttcat aataccgaag tcctttctgg
      841 aataataata tctcaactga ttcaccctgg aactccagtt atttatggtg ctgcttggac
      901 aacttatgaa atgaaacaag ccaatgtcct gattggacgg cctgaatctt ctttacttag
      961 aattgctggt gcccaaatgg cacactatta taacatcccc agtcatacaa ccgcaccaga
     1021 caacgatgcc aattcacatg atgaacaggc tgcatgggaa aaaatgcttt caacccttgc
     1081 cggaatcatt ggcgctaatg acatgattgt caatttagga atgtttggaa caggaatgac
     1141 tatcagcctg gagcaactgg ttatggacaa tgaaatgtgt cgaatagcca aacgattccg
     1201 tcaaggaatc gaggtaaata aagatacact agctcttgac tctattcttc aagtcggacc
     1261 acgaggtgaa tatctcactt cggaacacac cctccatcat ttgcgtagtg gtgagcatgt
     1321 ccctcttgat gtgtcaaatg gaacaaattt tgaagtttgg aagcaaaaag gatcaaaaaa
     1381 cactacggaa aaatcctctg atttggtatc atccatcctt ctcggtggct gtcagaatcc
     1441 actttctcaa gacaaacaaa aagcaattca atctattatc aaccaatatg aaaaaaaact
     1501 aggcctacga tagatagcat tgtttaagtt aacaataaat ttaaaaaagg agagaaaatt
     1561 atgacacccc ggattggatt aattggaata gcaggaccct tagaagttgg tttttcagaa
     1621 actccgtaca ttctccaaag aatgaaaaac caactggaaa atattgcccc tgttataacc
     1681 aatttggata ttatatatga cctccaaacc gttcaaaaag ctgttaattt tttcgccaat
     1741 catgatttcg atctcctctg cgtaggagtt ggaacctgga gtgaagatca ccatttgctt
     1801 gattttttag aatattataa tcgaccggtg gttctttggg ctttaccagc tgtcgaaact
     1861 ggttcattat gtggtgttca gcaaatttgt tgtgttttaa aagaattaga aagaccctac
     1921 tttttcgttt acggagagcc agaggatgtc aaagttcatc aaaaaattcg ggaaattgca
     1981 atggcggtaa ccctgggaaa tcatcttcgc cattctcgta ttggtgtcat tggcggacga
     2041 gtgaagggaa tgaccgaaat tgcttacgat gagttagaaa ttaaggctcg aactggagtt
     2101 cgtatcgtga acttagacga agatgagctt attgaatcag ttaaaaatac cgatcaactt
     2161 gccgcttcta aactttggac tgcaattggg gaaaaagtcg ggaagataac agttaataat
     2221 caaactggca tcgaggctct cagctattat ttcggcttaa aaaaactaat tgaagaatac
     2281 gatttagatg gaatatgtat aaaatgttac ccacgatata tgggaaaaat ttgcctggcc
     2341 tattcgctcc tagccgaaga ggagattgtt ggtggctgtg aaggagatgt caataatact
     2401 gtaaccatga aaatcctttt taatttaacc ggaaaaccgg ttcacaatac tgatttccta
     2461 tatccagatc caatttcaca aacagtttta ttttcccatt gcggttcagg aggttttagt
     2521 attgcaaatg atcctgctca aatagagctt tcaccagttc gtttgctcaa tcagggagtg
     2581 tgtgctttat ttccagcgcg gataggtgtt gtcactctgg ttaacttagt aggaaggagg
     2641 ggaacttttc gaatgtcagc actaaccgga gaggcaatag agtgtgggat ggaatttcct
     2701 ggaaatcctc taaaagtgcg cttccaacgt tctttggaag atttgaatga agaaattgca
     2761 tctcaaggga ttggacatca ttggatggct ggttatggtg atgttcgtaa ggagcttgaa
     2821 tttttctgtt cattgaataa gatccaatat tattcactgt aaggtgaagg agtgggtatt
     2881 ttttaaaatt tgttaggtgt taaatatatt taaatatttt ttaaacagga gggataatca
     2941 atgtttaagc agaagttttt ttctcttttt ttagctattg tctttgttgc agtcagttta
     3001 ttatttagct tagcttatgc ccaggaaact ttgaacattc tttgtttcca aggttacacc
     3061 gaagatgttt gggttaagga atttgaaaga ctaactggcg taaaagtaaa tgctacttat
     3121 tctggcatgg ttgaagaaat gttcaccaaa gctgcggctg gtggtgggca gtatgactta
     3181 gtcaccattg attgcggttc agttcaaaga tattacgaga ataatcttct ccagccaatc
     3241 gatttgagca aaatttctaa ttgggaaaag gtaagccaaa aattcaaaga tattgacttc
     3301 acagtatttg atggaaagcc gtatcatgtt tcattcgttt ggggttcgaa taatgttgtt
     3361 tacaataaag atgccgtggg tgaattaccc actacctggt cagtcttatg ggaccccaaa
     3421 tacaaaggtc aagtttcaat caccgatgaa gcgaataaca atgttgttgt tgcagctata
     3481 gctctcggtt tccccgatcc ctacaatctc actgaagatc aatttaagca agtgaaggaa
     3541 aaattgatag aaattaaaag aaacttgcgt actctgaccg ttggatttga ttcagagaaa
     3601 agtgttcttg gttctggtga aaccaatcta agcgtatctg gttatgattc tggtctgatc
     3661 ctctatctcc gagacgaact gaaaatgaac gttggtcgcc ttaccccaaa agaaggcatt
     3721 tatgtatggg ctgatggctg ggttatttta aaagattcaa aaaatcctga tctagcccat
     3781 aagtggattg acttcatgtt atctgatttt gcccaaaaag aactggctaa gtatatggga
     3841 tttggagcag ttacttctgc agccaaagaa gttattgacc ctgatattgt taaaatgtgc
     3901 aactatgacg atatagataa tgttccagtt ccggtctttt taatgaaaag cccagaggat
     3961 tttgaagctc gggtgaacct ctggaacgaa gtcaaggcta ctcagtaaac atttaaaggg
     4021 taggggaaca gtgaaaatta tctgattcat tttcactgtt cccgtcttta gttttttagc
     4081 aaatatttca ttgcttttta aattggtcaa ttaaaaattg atcaatttaa tgaattgaga
     4141 tctattggaa aattgtggga ttgtgtaaaa ataaattctt aaattaataa accaaacatt
     4201 tcccaatcga ggtttagaat gaacaaccat gagacagaaa tacaactcat tcagattact
     4261 aagaaatttg gtaattttac tgcggttcaa aatgtcaatt tagagatcca tcatggagag
     4321 tttttctctt tacttggacc ttctggctgt ggtaaaacca ccacattacg aatgatcgct
     4381 ggccttgaag agcctactga gggccaaatt tgcttagtcg gagaagatgt tacttataaa
     4441 cctgcgtatt tgcgcaacgt gaacacggtc ttccaggatt atgctctttt tcctcatatg
     4501 accgttgaag aaaatatcca tttcccattg aaaatgaaaa aggtgaaata ttccagcgct
     4561 cggacaatga ttgaagaagc tctttctttg gtaaatatga ccgggtttgg caaacgttat
     4621 cctcaacaac tttccggtgg acaacgtcag cgaatcgcct tggcacggtc gctggtcaat
     4681 cgtcctcgcg cacttttact cgacgaaccc ttgggggctt tagattttaa acttcgaatt
     4741 gctatgcaaa aagttctaaa agatattcaa aaaaatgtgg atatcacctt tatttatgta
     4801 actcacgatc aaactgaagc aatcaccatg tcggatcgaa tctgtgtcat gaaagacgga
     4861 ttaatccacc aaattggtac tcctgatgaa atttacaatt ctcctgcatc cacttttgtt
     4921 gccagtttta ttggtgatat gaactttctt gaaggtcaag tagtccaaaa aggagaaaat
     4981 tcaatcacta tcgatgtaga tggcaaaccg attctcagca cgcgttttaa aggaagtttt
     5041 tcaaaaggag attcagttgt tgcctgcatt cggccggaaa aaatatgttt agataaagtg
     5101 tgcgatagtg agtttcagaa taaatttgaa aatcatttcc aagctgcaat ccacagaatt
     5161 gttttccggg gaactgatta tgaaatcact ctcaaatttg gacaatcaga aattcgcttg
     5221 atagaaagcg cttcggattt taatgataaa aaaattggtg atcaactttt tattggtttc
     5281 cagaaagatg atgtgattac ttatttaaag gaatgtaaaa aggtatgtga ttatgaaacc
     5341 aaccttgaca actaaattga atgccagacc aagagggcaa tcttcctttt tttccaaaat
     5401 ttggattggt gtccctataa ccacctggct gtttttatta atcatcattc caatctgcat
     5461 tatgctggtt accagtttct atatcaaaga aggctctgtg gtggttaaaa aaattagcct
     5521 ggtgaactat ttagaatttt ttataaatcc aatctacctt cctatcttct ttcgaaccct
     5581 cctcttagca cttttagtat ctcttacttc aatattatta ggatatcctt tggcttattt
     5641 agtatcaaga aaagtaaaac gctttcgaaa tcaactctat atgctggtca ttgttccttt
     5701 atgggtaagt tatctggttc ggatcgtggc ctggcgaact atattaggac gagttggggt
     5761 cctcaatacc attctccttt cactaaaaat tatcgatgaa ccactctcca tctttatcta
     5821 tagtcctttt gctgtttatg tcgctctcat ctatattgcc ttaccctatg tgtttatttc
     5881 tatatttaat tccttggaaa aaatccccaa aaatctttta gacgcctccg ctgacctggg
     5941 tgctaatagt tttaatacct ttctccatgt cattcttcct ctttctatgc caggagtaat
     6001 tagcggtttt actcttgctt ttgttatcgc tcttggtgat tatattattc cccagcaatt
     6061 gggaggtttg aatggaatga tgttcggtaa tctcattgtc actcagtttg gttatgctta
     6121 taactggccg ttaggagcag cattaggctt cataatgttt acagttgcag tcgttatcct
     6181 actagtttca caaaaatttg gttcatcaga agggttttta gaataatgaa aaagacatcg
     6241 aatctaaaaa gtaagaataa atctttccct tttttgagta tttatggaac tttagtgtat
     6301 ttttatctct atttcccttt gttggtcgtt tttgtttatt cactcaactc ctctcgctca
     6361 accatgaagt ttgaagggat aacgtttgat tggtatatga aactctttca aaataaagaa
     6421 ctattaaact cactttttca ctcccttcag gtttcaggat tggcagttct ttttgcagta
     6481 gtcatgggaa catcgggggc gcttttcctt aaccgaattg aattcaaggg aaaagacttt
     6541 tttcgcacgc tggctatgct tcccatcatc ctccccggaa taatagttgg tctttcttta
     6601 ttgatatttt ttctcaattt gaagctacag ttatcaatgt ggaccgtcat ccttgggcat
     6661 atgagcttta ctacaccagt cgttatgttt caggtcttag ctcgcttagc acgtctttct
     6721 agaaatttag aaaatgctgc catggaccta ggcgccaatc cattccaagc ttttatttat
     6781 gtaacctttc ccatgatcaa aagagcagtc attgggggag cactgctggc ttttaccatg
     6841 tcttttgatg aaatcatcat aacttatttt cttaccagta cctacaatac tcttcccatc
     6901 tatctttatg gcatgcttcg ctttggtctt tctcctgagg tttatgcgat ctcgacggtt
     6961 gtacttggtt tatccatcct attaataatt ctcatggcaa aatataccgg gagcggagag
     7021 gaatcaattg ttgtccgtta atggaattat ttatcttgcg tgtctacctc ctcctcttct
     7081 tctttggttt ttttgctttt aaatgggcat tgggaacaat catcatcctg aagatacccg
     7141 gttattgaat actctggctg aatgagttga tcggcctttt tcaatattat gattgagata
     7201 agtatggcta ttatggtaac agccccgatc aacataactt gaggggttaa agctttaact
     7261 ataagatacg taccta
//
LOCUS       DAOSOL010000031         7218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10371_length_7218_cov_4.298478, whole genome shotgun sequence.
ACCESSION   DAOSOL010000031 DAOSOL010000000
VERSION     DAOSOL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_7218_cov_4.298478"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="PK171_01470"
     CDS             <1..956
                     /locus_tag="PK171_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT04905.1"
                     /translation="GYPGLQLTKTTIKQNQFNHIVQFQSLSRLYDRFQPDSMFFMMDL
                     SVEASALGLAVRFPLEETPSVEDHPVKTHEDLRGFRKIDILGDGRVSVFIETLHHMRS
                     AFPCPVGAYVIGPLTLAGLLIGANELAIKSLIEPELFRETVEFSYQVVMRYTQALRDA
                     GADMIMVLEPTAVIFGPDQFRQNLTSLYREMVGIFDDIEIILHVCGDSTHLLKVMADT
                     GVAGLSLDSMVDLKKAAEIVGPQVMLIGNLSPIEMLTANPNEVYIQTMEILEKTKSHP
                     PFVLSTGCDLPQDVPLENIDAFIKAGKDWRQESVFKKVEAN"
     gene            complement(953..1369)
                     /locus_tag="PK171_01475"
     CDS             complement(953..1369)
                     /locus_tag="PK171_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04906.1"
                     /translation="MLIIAHSLWGLTLATQTQNIYLLLLGGVGGHILLDAIPHRDLTT
                     FGQVIIDIIIAATFSLFCLKIFSLPFVFAWSVLFSTLPDVEVAFRYYSKWKGVYFPSH
                     LPSWHGTLNSHLGILMNLFIGLVLLMVFLINIKKVL"
     gene            complement(1572..2438)
                     /locus_tag="PK171_01480"
     CDS             complement(1572..2438)
                     /locus_tag="PK171_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT04907.1"
                     /translation="MPLVSTKYLIELAEKKEFALPAFNSPHLEFMTWILEEAEKLHSP
                     VIIQFAPVEYYFLDIAALSDALHALSRKFSVPFSLHLDHSHEIKEVMDAILNHFSSVM
                     IDGSRHSIEKNIEITKKVVEISHLAEVIVEGEIGRVGGLEGDEQWEVDPESDDFFTLP
                     EEAAFFANKTGVDLLAVAVGTRHGLYNGMVPRLQFDRIREIKKQTHLPLVIHGGSGTP
                     VDQLQQAVRAGVRKINFSTTLRVAFLKSIREYTDHHPDELFLPKIFRDISPSLKAAAR
                     ECMESSLSVGKA"
     gene            complement(2576..3811)
                     /locus_tag="PK171_01485"
     CDS             complement(2576..3811)
                     /locus_tag="PK171_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04908.1"
                     /translation="MPKIFLLDVTNRDGVQTAHLGLAKIEKTIVNMYLNEMGVTQSEF
                     GFPTTHHETNYLNANLELADMGVLCPTRLSGWLRAVKKDVEVAYQLVPKLKYLNLSIS
                     TSEQMLSGKFQGRMTRDDVIHSMTEATQAALQLGAVGVGVNAEDASRADVEFLIDFAL
                     AGKEAGAERFRYCDTLGYDSPFSIYDRIIKIAEASKMPIELHCHNDLGMAVANSISGA
                     KAAIDAGCDVYINTTINGIGERSGNADLITVILAIKYANYFDNENYSFLENVNLKAAW
                     KTCKYASYAFKVPIPITQPGVGANAFAHASGIHADGVIKDRKNYELYDYEELGRGEPE
                     LIVTGRQIVVGEYSGIRGFRNVYDNLEVEFKNEEEARKILELTRFANVEKQKPLVEEE
                     LLFIASYPEQARKILTLTP"
     gene            complement(3828..5018)
                     /locus_tag="PK171_01490"
     CDS             complement(3828..5018)
                     /locus_tag="PK171_01490"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT04909.1"
                     /translation="MKISQKVNQIEPSATLAISARAKTMKKNGLNVLSFSAGEPDFDT
                     PQCIKDAAKLAIDQGFTKYTPTSGIIELKEAICYKLNKENGLAYHPDEVIVSCGAKHS
                     LFNAILTLCDTEDEVLLPVPFWVTYVEQIRLAGGKPVFIHCDPKTLRISWEDILSKIT
                     NKTKLFILNNPSNPSGIVWDIEILKKIADLAVEKDIIIISDEIYENLIYDGAQVKSIA
                     SFSPEAQTQTIIINGVSKTFSMTGWRIGYTAGPKAAIQAMGRLQDHSTSNPTSISQKA
                     ALAALQCDQQIVQNMVKTFNQRRLLMIQLLNEIPDITFPVPQGAFYVFADFSSYLGRY
                     FENQRIDTSQQLTEILLEKALIAAVPGSAFGMEGYLRFSYATSEESIEKGMTQLKNFL
                     QNIT"
     gene            5622..5915
                     /locus_tag="PK171_01495"
     CDS             5622..5915
                     /locus_tag="PK171_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04910.1"
                     /translation="MDYKVMLDTIWVIIASMLVFFMNLGFAMVEPGFNRSKKHCQYPF
                     KEFYVFFAVSTLGFLVLGWVLMFGSGGGAIHCAPTILYSLVGGLIYHAREFSG"
     gene            complement(5925..6527)
                     /gene="rdgB"
                     /locus_tag="PK171_01500"
     CDS             complement(5925..6527)
                     /gene="rdgB"
                     /locus_tag="PK171_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOT04911.1"
                     /translation="MKPASRLIYILSKNSGKIKEINAILSSYSISVRSLFQDFSFEEV
                     AETGSSYAENALLKAHHGFKISENICIGEDSGLEVDALDGAPGLFSARFGGETLDSKE
                     KNKQILELLKKVPQDQRSACFVCVVALVWEKSEKIFEGRCPGLISQESRGSSGFGYDP
                     IFILPPYQKTFAELGENIKNRLSHRAIAFRKLAEFLLSNM"
     gene            complement(6524..>7218)
                     /gene="rph"
                     /locus_tag="PK171_01505"
     CDS             complement(6524..>7218)
                     /gene="rph"
                     /locus_tag="PK171_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOT04912.1"
                     /translation="LMITRNYLRAAEGSVLIEEGNNRIVCSATIEDKVPLFLRGSNQG
                     WITAEYAMIPRATSTRNVRESIKGRVGGRTHEIQRLIGRALRSVMNMDLIGERTILID
                     CDVIQADGGTRTLAINGSFIALVDALWNYVDRGLLQSGNLLKDYLAAVSVGVVNGKDL
                     LDLNFEEDSMAQVDMNVVMTGKGQFVEIQGTAEGDPFSQETLNRLLALAQKGIKDIIA
                     IQKKVLLERGLP"
BASE COUNT         2181 a         1397 c         1413 g         2227 t
ORIGIN      
        1 tgggttatcc aggcctccaa ttaactaaaa cgactataaa acaaaatcag tttaatcata
       61 tcgttcaatt tcagtcactc agtcggcttt atgatcgttt tcaacccgat tccatgtttt
      121 ttatgatgga tctttctgtt gaagccagcg cattaggatt ggcggtccgt tttcccttag
      181 aagaaactcc atcggttgaa gatcatccgg taaaaaccca tgaagattta agaggatttc
      241 gtaaaataga tattttaggc gatggtcgcg tttcagtatt tattgaaaca ctccatcata
      301 tgcggagtgc attcccctgc ccggtaggag cttatgtcat tggaccgctt accctggcgg
      361 gtttacttat tggagctaat gaattagcga ttaaaagcct gattgaacca gaactgtttc
      421 gtgaaactgt cgagttttct tatcaagttg taatgagata tacacaggct ctcagggacg
      481 ctggtgctga tatgataatg gtattagaac caacggcagt catttttggg ccggatcaat
      541 tccgtcaaaa cttgaccagt ctatatcggg aaatggtagg aatcttcgat gatattgaga
      601 ttattctcca tgtttgtggt gatagtaccc atttgttaaa agtaatggct gatacaggag
      661 tagcaggttt aagtttagat agtatggttg atctcaaaaa agcagcagaa atcgttggac
      721 cacaagtaat gttgattggt aatttgagcc cgatagaaat gcttactgcc aatccgaatg
      781 aggtttatat tcaaaccatg gaaatattag aaaaaacaaa aagccacccc ccttttgttc
      841 ttagcaccgg gtgtgatctt cctcaggatg ttcctcttga aaatattgat gcgtttatca
      901 aagcaggaaa agattggaga caagaatccg tttttaaaaa agtggaagcc aattagagta
      961 cttttttgat attgatgaga aaaaccatta ggagaaccaa tccaataaaa agattcatta
     1021 aaattcctag atgagaatta agagttccat gccaggacgg taaatgagag ggaaaataaa
     1081 ctcctttcca tttactataa tatcgaaatg caacttcgac gtcggggaga gttgaaaaaa
     1141 gaactgacca ggcaaaaaca aaaggaagag aaaatatctt caggcaaaaa agagaaaaag
     1201 tcgctgcaat tattatatca attatcactt gaccgaaggt ggtcaagtct cgatgaggga
     1261 ttgcatcgag gagaatgtgc ccccctaccc ctcctaaaag taaaaggtaa atattttgag
     1321 tttgagttgc taatgttaaa ccccaaagac tatgagcgat gatcagcata attcatccta
     1381 taaaattgta atttgtgata aatgaacagt aaaataaatg taatatttaa aagtgcaacc
     1441 aaccccgctt ctccgtgatg cccctccgtt ggaggggcat ttctagtcgt cattctaagg
     1501 aacgtattgt gcgacgtgag aatctcatct tttgtctttt ttgtcacata aagttagaat
     1561 ctattctgaa gttatgcctt cccgactgac aaggaagatt ccatacattc tcgagcagcg
     1621 gcttttaaac taggagaaat gtcccggaat attttgggta aaaagagttc atcaggatga
     1681 tgatcggtat actcgcgaat gctttttaag aaagccacgc gcaacgtagt gctgaagttg
     1741 atttttctca ctccggcacg gactgcttgt tgaagctgat caactggagt cccacttcca
     1801 ccatggataa ctaagggtaa atgcgtctgt ttttttattt cacgaatacg atcaaattgt
     1861 agtcggggta ccatgccatt ataaagtcca tgacgggttc caaccgcaac cgctaacaaa
     1921 tcaacgccag ttttattggc aaaaaaggca gcctcttcag gaagagtgaa gaaatcgtca
     1981 gattcgggat cgacttccca ttgttcatct ccttccagtc cccctactcg gccaatttct
     2041 ccttcaacaa tcacctcagc aagatgagaa atttcaacca cttttttggt aatctcgata
     2101 tttttctcaa ttgagtgtct tgacccatcg atcattaccg aagaaaagtg atttaatatg
     2161 gcgtccatga cttctttaat ttcatgacta tggtcgagat gaagagaaaa tgggactgaa
     2221 aactttcgag aaagagcatg aagggcatca gaaagagcgg caatatctaa aaaataatat
     2281 tctactggag cgaactgaat gatgaccgga gaatgtaatt tttccgcctc ttctaatatc
     2341 caagtcatga attctaaatg gggagagtta aaagcaggaa gtgcaaattc ctttttttcc
     2401 gctaattcaa taagatattt cgttgaaacc agtggcaatt gtgattactc cttccttaaa
     2461 agagaaaaaa tttggtaatt atgcaaacgg atatataata cgagtataga gctgaaaata
     2521 caagtggatg gatttaaaaa aaagagcggg gatttaaccc cgctcaaatt agtttttagg
     2581 gagtcagggt gagaattttt cttgcttgtt ctggatagga agctataaac aacagttctt
     2641 cttcaaccag gggtttttgt ttttcaacat tagcaaatcg agtaagctcg agaatttttc
     2701 tagcctcttc ctcgtttttg aattcaactt ccaaattatc ataaacatta cgaaaaccac
     2761 gtataccact atattcaccc acaacgattt ggcgaccagt tacaatcagc tccggctccc
     2821 cacgccctaa ttcttcataa tcataaagct cataattttt ccgatctttg atgactccat
     2881 cagcatgaat accagaagca tgagcaaaag cattggctcc tactcctggt tgagttattg
     2941 gaatgggaac cttaaaagca taagaagcgt atttacaggt tttccaagcg gctttgagat
     3001 ttacattttc aagaaacgag taattctcat tgtcaaaata gttggcatat ttaatcgcca
     3061 aaatgacagt aatcagatca gcattgccag atcgctctcc aattccatta attgttgtgt
     3121 taatatagac atcacatcca gcatcaattg ctgctttggc gccactgatc gaattggcga
     3181 ccgccattcc caaatcgtta tgacaatgaa gttcaatagg catttttgag gcttcggcaa
     3241 tcttaataat tctatcataa atactaaaag ggctgtcata gccaagagtg tcacaataac
     3301 gaaatctctc agctccggct tccttccctg ctaaggcaaa atcaataagg aattcgacgt
     3361 cagcccgtga agcatcttca gcattcaccc ccaccccaac cgcacccaac tgtaatgcag
     3421 cttgggtcgc ttctgtcata gaatgaataa catcatcacg agtcattcgt ccttggaatt
     3481 ttcctgaaag catttgttcc gatgtggaaa tggataagtt gagatatttt aactttggaa
     3541 ccagttgata tgccacctct acatcttttt tgaccgcccg aagccatcca cttaaacggg
     3601 tcgggcataa aactcccata tcagccaatt caagattggc attcaaataa ttggtttcat
     3661 gatgggtggt tgggaaacca aattcacttt gggtaacacc catctcatta agatacatgt
     3721 tgacaatagt tttttctatt ttggctaaac ctaaatgcgc ggtttgcacc ccatcacgat
     3781 tggttacatc aagtaaaaag atttttggca tgtcgtcgat acccccttta agtaatgttt
     3841 tgtaaaaagt ttttcagttg agtcattcct ttttctatgc tttcttctga agtggcataa
     3901 gaaaaacgca gatatccttc cattccaaaa gcactaccag gcacagcagc aatgagggct
     3961 ttttctaaaa ggatctcggt aagttgttgt gaagtgtcga ttcgttgatt ctcaaagtat
     4021 cttccaagat aagaggaaaa gtcggcaaaa acatagaaag caccttgagg aactggaaaa
     4081 gttatatcag gaatttcatt taaaagttga atcatcaaca atcttctttg attaaatgtt
     4141 tttaccatat tctgtactat ttgctgatca cattgcaaag cagctaaggc agccttttga
     4201 ctaatagagg tcggattgga tgtggagtga tcttgcaaac gtcccatggc ttgtattgct
     4261 gcttttgggc cggctgtgta accaattctc catccggtca tggagaaagt cttagaaaca
     4321 ccgttgataa tgatggtttg cgtttgagct tctggagaaa aggaagctat acttttaacc
     4381 tgagctccat cgtatatcaa attttcatat atctcatcgg aaattattat gatatctttt
     4441 tcaacagcaa gatcggcaat tttcttcaaa atttcaatat cccaaactat tccgctcggg
     4501 ttagaaggat tatttaagat aaataacttg gttttattag taattttgct aaggatatct
     4561 tcccaactta tccgaagtgt tttgggatcg caatgaataa aaacgggttt ccctcctgcc
     4621 aaacggattt gttcgacgta agtcacccaa aagggaacag gaagaagaac ttcatcttcg
     4681 gtatcacaaa gggtcaggat agcattaaaa agtgaatgtt tggctccaca agaaacaatc
     4741 acttcatcag gatggtaggc aagaccattc tctttattta atttatagca aatcgcttct
     4801 ttcaattcga tgattcccga tgtcggagta tatttggtaa atccctgatc aatagctaac
     4861 tttgcggcat ccttaataca ttgaggggta tcaaaatcag gttctccagc actaaaggat
     4921 agaacgttca atccgttttt cttcatggtt tttgctcgag ctgatatagc aagagttgcc
     4981 gacggttcaa tttgattgac tttttgggat attttcactg tgtgccccct attttggttt
     5041 taattttata tataggtggt gggtaacgct ggtattggag ctaaaaaatt ttatttcggt
     5101 tccacggatt cggtttggag tgattcattt cgacgattat gggctgaata taatattagt
     5161 atttcaaaag aaaagagtga aaaatctgcc ctagcccgtt ttcactataa aaagatttta
     5221 ttcactctta tatagtagta aggagtaatt gtcaagaatc tgagcaattt tttatcgatg
     5281 atttaatggg tattggtttt ttaagattaa aaaaaatttt tggtattgat ttgtttctat
     5341 tattcaactt cttgacatga attcattgaa tatggtagaa attgtatcat agcaatgaag
     5401 ctcttcccac gatgttgtgg gaagagcttt ttttatttta tcggttttac caaggtaata
     5461 caatggagtg ttacccgatt aacgctttct ttccctcaga gttttgaaga aagaccttct
     5521 cacactacag tgattgaatt tattccttta ttgtctcagc gaagagattc ttaatcaacc
     5581 gaatatccat tattttaaac tttgtcaagg gggatttaag aatggactat aaggtaatgc
     5641 tagataccat ttgggtaatt attgcttcaa tgttggtatt ttttatgaat cttggttttg
     5701 ccatggtgga accgggtttt aatcggtcaa aaaaacactg tcaatatcct ttcaaagaat
     5761 tttatgtttt ttttgctgta tcaacattag gatttctcgt tcttggttgg gttttaatgt
     5821 ttggaagtgg tggcggtgca atacattgtg cccctacaat tttatattct ttggtagggg
     5881 gcttgattta tcatgcccgt gagttttcag gatagaaggc ttttttacat attagataga
     5941 agaaattctg ccaatttgcg aaaggcaatt gctctatggc tcaagcggtt ttttatattt
     6001 tcgcctaact cggcaaaagt tttttgataa ggaggaagta taaagatggg gtcataacca
     6061 aagcccgaac ttccacgact ttcctgagaa ataagtcccg gacaacgacc ttcaaaaatt
     6121 ttttcacttt tttcccatac caaggctact acacagacaa aacaagctga tctttgatct
     6181 tgggggactt ttttaagaag ctcaagtatt tgcttatttt tttcttttga atccaaggtt
     6241 tctccaccaa agcgggccga aaaaagccct ggagctccgt caagagcatc aacttctaaa
     6301 ccggaatctt ccccaataca tatattttca ctgattttaa aaccatgatg ggcttttaga
     6361 agggcatttt cagcataact cgatccagtt tcggcaactt cctcaaaaga aaaatcttga
     6421 aataagctcc taaccgatat tgaataagag gataaaatcg cattgatttc tttaatcttt
     6481 cccgaatttt tagaaaggat gtaaatcaat cgggaagcgg gtttcaagga aggccccttt
     6541 ctagtaaaac cttcttttgg atagctataa tatctttaat tcccttttgg gctaaggcaa
     6601 gaagccgatt cagggtttct tggctaaagg gatccccctc agcagtaccc tgaatttcaa
     6661 caaattgtcc cttacccgtc attacaacat tcatatcaac ctgagccatc gaatcttctt
     6721 caaaattgag gtcaaggaga tctttaccgt tgactacccc cacgctcaca gcagctaaat
     6781 aatctttcag aagattacct gattggagta aacctcgatc aacgtaattc cagagggcat
     6841 cgactaaagc aatgaaagat ccgttgattg ccagggtacg ggttcctccg tcagcttgaa
     6901 tcacatcaca atctataaga atagttcgtt cgccaataag gtccatattc atcactgaac
     6961 gaagggcacg accaataagc cgttggattt catgggtacg acccccaact cgacctttga
     7021 tagattcacg aacattcctt gtcgatgtgg ctcgagggat catggcgtac tcagcagtaa
     7081 tccacccttg gttggatcct cgcagaaaga ggggcacttt atcttctatg gttgccgaac
     7141 agacaatacg attgttccct tcttcaatta aaactgaacc ttcagctgct ctaagataat
     7201 ttctggtgat catgagag
//
LOCUS       DAOSOL010000032         7213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10381_length_7213_cov_4.594579, whole genome shotgun sequence.
ACCESSION   DAOSOL010000032 DAOSOL010000000
VERSION     DAOSOL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_7213_cov_4.594579"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PK171_01510"
     CDS             <1..874
                     /locus_tag="PK171_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT04913.1"
                     /translation="KKGRTFSKLNIINVLYPHSLRRFLGFPLYQEILEGIAFAAQQLN
                     YQIIIATSGQDFHPTTIYDEMLNTDLGDGYLILELLKRDSRLKRFTQGIKPFVTIGKP
                     EIQDTRGVSWVSIDVTAMVEKAVRKLINDGHQHIAFLGLEDKRIITLQAQRGFERALD
                     KEKIAKRDMTILHLEPDQYRTESVVKNFLKKSSDITAFFTESQILAEGVIRAAKKLGR
                     SIPGDLSIMSMLENQASNFVEPSLSGIDWKAKDLGEQAVMLLVKIIEGRENGNSGRTI
                     TSELIIRESCGIKI"
     gene            complement(939..1433)
                     /locus_tag="PK171_01515"
     CDS             complement(939..1433)
                     /locus_tag="PK171_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOT04914.1"
                     /translation="MIIKGKVIQYGDNVDTDVIIPARYLTATDPLELAKHCMEDLDPD
                     FQKKLDNNSIIVAGKNFGCGSSREHAPLAIKGSGIKAIIASSFARIFYRNAINIGLPI
                     LELEEAKIRLLSEDEIDIDIQRGTITNLTQNKIYQAQPFPPFIQEIIARGGLMNLVKE
                     RMST"
     gene            complement(1541..2806)
                     /gene="leuC"
                     /locus_tag="PK171_01520"
     CDS             complement(1541..2806)
                     /gene="leuC"
                     /locus_tag="PK171_01520"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOT04915.1"
                     /translation="MGMTITEKIIAAHSGKEKVSAGELVQVSIDLALANDITAPLSIK
                     ELYKYRIDSVFNPDKVVLVADHNTPAKDIASAQNLKLMREFAQKNQIKNFFEGGSVGI
                     EHVLLPEKGLVLPGDLVIGADSHTCTYGALGAFSTGMGSTDIAAAWALGETWLKVPES
                     IKFIYSGQPGQFVTGKDFILFIIGEIGVEGARYCSMEFTGSGISTLSMDGRFTMANMA
                     VEAGAKNGIMEADAITLSMLSNISLSRKPRIYHSDSDAEWKDVIEVNVASIEPQVALP
                     HLPENTKSVFSLPHIAINQSVIGSCTNGRIEDLRQAAEVLKNRQVHPGVRLYIFPGTP
                     SILLQAEREGLVKIFFESGAVLCPPSCGPCLGGHLGVLAEGERCISTTNRNFVGRMGH
                     TKSEVYLANPYIAASSAIAGYITNPDQLS"
     gene            complement(2910..4442)
                     /locus_tag="PK171_01525"
     CDS             complement(2910..4442)
                     /locus_tag="PK171_01525"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOT04916.1"
                     /translation="MKKVRILDTTLRDGEQSPGVSLNVQEKLEIARQLARLNVDVIEA
                     GFAVASSGDALAVKSIAQEVKGPIISSLARSKEKDIDIAWESVRFSERPAIHTFIATS
                     DIHLKYKLRMSREQALEQAVWAVKRARNYVGEVEFSAEDATRSDWDYLCQVYSAVIKA
                     GAMVLNVPDTVGYTTPEEMKALIQHLYKHTDGIEKVIVSVHCHNDLGLAVSNSLSAML
                     GGAGQIECTINGIGERAGNASLEEIVMGLHVRKDFYQTETGINYSEIYRTSRLISQLT
                     GMLVQPNKAVVGQNAFAHESGIHQDGVLKEKSTYEIMDAKLIGREEKVLVLGKHSGRH
                     AFNNKLEELGYHLSEEELNQAFERFKALADQKKQIAEADLQFIAADEATRTEEVYRLD
                     FVHVCCGNNILPTATVRLVHENGSTLQGVATGVGPVDAAYKAIGQMVGLSSNLVNFTL
                     QSISGGTEALGEVVVRIFTDDTVYVGRGSHQDVVVASVIAYLSAINKMTQQKKLNPQT
                     HL"
     gene            complement(4459..5451)
                     /gene="ilvC"
                     /locus_tag="PK171_01530"
     CDS             complement(4459..5451)
                     /gene="ilvC"
                     /locus_tag="PK171_01530"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOT04917.1"
                     /translation="MARIFYDQDAQLELLQGKKVSIIGFGSQGSAQGQNLRDSGIEVI
                     IAELPGTPGYEKAKKVGFSPVSAEEAAQKGDIIQMLVPDQFQPMVYQQSIAKNLSSGK
                     ALMFSHGFNIHFHQIVPPPSVDVIMVAPKGPGDLVRRMYQEGKGVPSLIAVYQNASGK
                     AKELALAYARGIGATRAGVLETTFEEETETDLFGEQAVLCGGLTALIKAGFETLLEAG
                     YQPEVAYFECLHEMKLIVDLVYEGGLSLMRQVVSDTAEFGDLTRGPRVINQETKIEMK
                     KILTEIQNGSFAREWILENQANRPTYHALREKDFQHTIEKVGKQLRDMMPWLKK"
     gene            complement(5507..5992)
                     /gene="ilvN"
                     /locus_tag="PK171_01535"
     CDS             complement(5507..5992)
                     /gene="ilvN"
                     /locus_tag="PK171_01535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOT04918.1"
                     /translation="MQHIISVLVENKPRVLARVASLFARRGYNIESLAVGHTQDPDVS
                     RITLVVRGDEDILEQITKQLYKLIEVIKVGDFTEISYVDRELSLIKVNAPSNLRGEIV
                     QTAEIFRARIVDVSEKSLLIEVTGTSDKIDALKQLMKKYGIIEMTRTGKIALARGGQS
                     L"
     gene            complement(6013..>7213)
                     /locus_tag="PK171_01540"
     CDS             complement(6013..>7213)
                     /locus_tag="PK171_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT04919.1"
                     /translation="LIDIPKDVQIAEIDFNYPEKLEIPGYKPTYRGHFNQINMTAQAI
                     KEATRPLFYVGGGVIASGASDILLEVVEKTDIPVTYTLMAKGAIPEDHRLSLGWLGMH
                     GSYHANKAIMNCDLLIAVGARFDDRVTGNTTTFANRAKIVHIDIDPAEIGKNVKINIP
                     IVGDARNVLNELNKRTEPKKHDEWLETIEKWKKEYTPKPPANNELVAQTIIESVYQAT
                     QGDAILVTDVGQHQMWAAKHFLCHRPRRWASSGGLGTMGFGLPAAIGAQIGNPDQTVI
                     LFSGDGSIMMKIQELVTAVNNCLPLKIFVLNNNYLGMVRQWQECFFDCRYSSTHIINP
                     DLANLAQAFGAEGIPVQNQEELDRAVPRVLEVKDHPVLVDCRISKETNVYPFVPPGKS
                     LDEMILE"
BASE COUNT         2112 a         1518 c         1401 g         2182 t
ORIGIN      
        1 aaagaaggga cgtacttttt caaaattgaa tattatcaat gttctttacc cccattcctt
       61 gcgccgattt ttaggttttc ccttatacca agaaatactt gaaggaattg cttttgctgc
      121 tcaacaatta aattatcaga taataattgc tacttcggga caggattttc atccaaccac
      181 gatatatgat gaaatgctca acaccgattt gggagatgga tatcttattt tagaactact
      241 taaaagagat agtcgtttaa agcgttttac ccaaggcatt aaaccatttg taacgatagg
      301 aaagccagaa attcaagata ctcgaggagt aagttgggtt tctatagatg tgactgccat
      361 ggtggaaaaa gcagttcgta aacttataaa tgatggccat cagcatatag cttttttagg
      421 attagaggat aaaaggatta taacccttca agctcaaaga ggttttgaaa gagccttaga
      481 taaagagaag atcgctaaaa gagatatgac tatattacat ctggaaccag atcaatatcg
      541 aaccgagtca gtagtaaaaa acttcttaaa aaaaagctcg gatattacag ctttttttac
      601 agaaagccaa attttagccg aaggggtaat tagggctgca aaaaaacttg gcagaagtat
      661 cccaggtgat ttatcaataa tgagtatgct ggaaaatcag gcttcgaatt ttgttgagcc
      721 atctctttct ggaatagact ggaaagccaa agacttagga gaacaagcag ttatgctttt
      781 ggttaaaata attgaaggac gagaaaatgg caattccggt cgaacaatta cttctgaatt
      841 aattattcgg gaatcttgtg gaattaaaat ataaaagatt tgattataaa aacatttttt
      901 attagacagg gagaattatt aatctccctg tctaattatc aagtggacat tctttctttg
      961 acaagattca tcaagcctcc acgagcaata atctcttgaa taaaaggagg gaagggttgg
     1021 gcttggtaga ttttattttg agttaagttg gtaatagtcc ccctttgaat atcaatatcg
     1081 atctcatcct ctgagagtaa gcgtatttta gcttcttcca gttctaatat gggaagccca
     1141 atgttgattg cgtttcgata aaaaattctt gcaaaagaag aagcaataat tgcctttatt
     1201 cccgatccct taatggctag aggagcatgc tcgcgggaag aaccacaacc aaagtttttc
     1261 cctgcgacga ttattgagtt gttatccagt ttcttttgaa aatccggatc taagtcttcc
     1321 atacaatgct ttgccaactc taaaggatca gttgcagtta ggtaccgagc tgggataata
     1381 acatcagtgt caacattatc accgtattgg ataacctttc cttttataat cataaaatca
     1441 actccaacat agaaatgtaa gttttaaaga actgcctgat ttcaggtgat taaatactct
     1501 ttttagttat ttgaaaaatt ctttttatct aattcaataa tcatgaaagc tggtcgggat
     1561 tggtgatata gccagcgatg gctgagctgg cagcaatata gggatttgct aagtatactt
     1621 cgctcttggt gtgtcccatt ctcccaacga aattacgatt ggtggttgag atgcaacgtt
     1681 ctccctcagc taatacacct aaatgtccac caaggcaggg accacaggaa ggaggacaaa
     1741 gaaccgcacc cgattcaaaa aagattttta ccaaaccctc cctttctgct tgtaaaagaa
     1801 tggaaggagt tccagggaaa atatagagcc taactccagg atggacttgg cgatttttca
     1861 aaacttcagc agcctgcctt aagtcttcaa ttcggccatt ggtacatgaa ccaataactg
     1921 attgattaat agcaatgtgt ggcaatgaaa aaaccgattt ggtattctcg ggaagatgag
     1981 gaagggctac ttgaggttca attgaagcaa cgtttacctc gataacatct ttccattccg
     2041 cgtctgaatc tgaatgatat atccttggtt tcctcgatag agaaatatta gacagcatgg
     2101 ataaagtgat ggcatcagct tccatgatgc cattttttgc tccagcttcc actgccatat
     2161 tagccatagt gaaccgtcca tccatcgaca gagtggagat tcctgatcca gtaaattcca
     2221 tgctgcagta acgagctcct tcgactccta tttcaccaat gataaaaagg ataaaatcct
     2281 ttccggtaac aaactgtccg ggttggccac tatagataaa tttaattgat tccgggactt
     2341 taagccaggt ttctcctaaa gcccaagcag ctgcaatatc agtactcccc atcccagtgg
     2401 aaaaagctcc caatgctcca taggtacaag tatgcgagtc ggctccaata accaggtctc
     2461 ctggcaagac taatcctttt tctgggagga gaacatgttc aatgcctacc gaacctcctt
     2521 cgaagaagtt tttaatctga tttttttgag caaactctct cattaatttt aaattttggg
     2581 cggaagcaat atctttggca ggagtattat gatcggcaac caatacaacc ttgtccggat
     2641 taaatactga atcaatacga tatttatata attcctttat gctcagtgga gcggtgatat
     2701 cgttagcaag agcaagatct atagaaacct gtactaactc acccgccgat accttttctt
     2761 tgccgctatg agcagcaatt atcttttcgg taattgtcat tcccatgggt aacgctcctg
     2821 ttataaaaat tattatcaag gacggtgaat ctttttaaaa gattcaccgt cctaagaaat
     2881 tcaaaaaata tttatggttc ctgttggatt tataagtggg tctgaggatt cagttttttc
     2941 tgttgagtca ttttgtttat agccgacaga taagcaatga cactggcaac tacaacatcc
     3001 tgatgagagc ctctcccaac atagacggta tcgtcagtga aaattcgaac cacaacttct
     3061 cctaatgctt cggttcctcc cgatatcgac tggagggtaa agttcactaa atttgaagaa
     3121 agtccaacca tctgaccaat tgctttatag gcagcatcaa ctggaccaac accagtagct
     3181 actccctgta aggttgagcc attttcgtgg acgagcctta ccgttgcggt tggtaagatg
     3241 ttattaccac agcaaacatg aacaaaatcc aagcggtaaa cttcttcggt tcgagtggcc
     3301 tcatcagcag caataaattg aagatctgct tcggcaatct gctttttctg atcagctaaa
     3361 gccttaaagc gctcgaaagc ttgattgagt tcttcttcgg aaagatgata tccgagttcc
     3421 tctagcttat tgttaaaagc gtgccggcca gaatgttttc ccagcaccaa gactttttcc
     3481 tcgcgcccga ttaatttagc atccatgatt tcataagtac ttttttcttt caatactcca
     3541 tcttgatgga tacctgattc atgggcaaag gcattttgac caactactgc tttattgggt
     3601 tggactagca tgccagtaag ttggctgatc aagcgactgg ttcgataaat ttcagaataa
     3661 ttaattccgg tctcagtttg atagaaatcc ttacgaacat gtaaacccat aacaatttct
     3721 tccaaagaag catttcctgc tcgttctcca attccgttga tggtacattc aatttgaccg
     3781 gcaccaccca acatagccga aagagaattt gaaaccgcta aacccagatc attatggcag
     3841 tgaacgctca ctattacctt ttctatccca tcagtgtgtt tataaagatg ctgaattaaa
     3901 gctttcatct cttcaggagt agtataccca accgtatcgg gtacgtttaa aaccattgct
     3961 ccggctttta ttactgccga gtatacttgg caaagataat cccagtcaga acgagttgca
     4021 tcttcagctg aaaactcaac ttcgcccaca taatttcgag cacgtttaac tgcccaaact
     4081 gcttgctcca gagcttgctc gcgactcatt cgaagcttat atttgaggtg gatatcggaa
     4141 gttgcaataa aagtatgaat ggcaggtcgc tcagaaaaac gcactgattc ccaagcaata
     4201 tcgatgtcct tttctttgga acgagctaaa gaactaatga ttggtccttt tacttcttgg
     4261 gctatgcttt taactgccag tgcgtcacca gaagaagcaa cggcaaaacc cgcctcaata
     4321 acatcaacat tgagacgggc taattgtctg gcaatttcta atttttcttg aacgtttaat
     4381 gatactcctg gggattgttc cccatctctt agcgtggtgt caagtattcg aacttttttc
     4441 acgctgtttc cctccctgtt atttttttaa ccagggcatc atatcacgca gctgttttcc
     4501 cactttttca atagtgtgct gaaagtcttt ttctcttaaa gcgtgatagg ttggtcggtt
     4561 agcctggttt tctaagatcc attcccgagc aaaactcccg ttttgtattt cagttaaaat
     4621 ctttttcatt tctattttgg tttcttgatt aatgactctt gggccacggg tcaaatcacc
     4681 aaattcggcg gtgtcactca cgacctgacg cattaaactt aggcctccct catacactaa
     4741 atcaacaatt aacttcatct catgaagaca ctcaaagtaa gcgacttcgg gttgatatcc
     4801 agcttccaaa agggtttcaa agccagcttt gattaatgcg gttaaacctc cgcataaaac
     4861 cgcttgttca ccaaagagat cggtttcagt ttcttcttcg aaggtggttt ctaaaacccc
     4921 tgctcgggta gctccgattc cacgagcata agccaaggcg agttctttgg ctttacctga
     4981 agcattttgg taaacggcaa tcaaagaagg aaccccttta ccttcttgat acattcttct
     5041 taccaaatcg cctggaccct tgggcgctac catgataaca tcgaccgaag gcggtggaac
     5101 gatttggtgg aaatgaatgt taaaaccatg tgagaacatc aaggctttcc cggatgagag
     5161 attttttgct atcgactgtt gatataccat cggttgaaat tgatctggta ccaacatctg
     5221 tataatatct cctttttgag ctgcttcttc ggcagaaact ggagaaaatc caaccttttt
     5281 cgctttttca taaccgggag tacccggtaa ctcagcaatg atgacctcga ttccactatc
     5341 tctaagattt tgaccttgtg cgcttccttg gcttccaaat ccgataattg atactttttt
     5401 tccttgtaac aattccaatt gagcgtcttg atcataaaaa attcgagcca taatgtgaac
     5461 ctccctaaag tataaattga ttaaatttta taagattttt agagaattat aaggattgac
     5521 ccccccgagc taaggcaatc ttaccggttc gagtcatttc gatgattccg tattttttca
     5581 tgagttgttt aagggcgtct attttatctg atgttccagt gacttcaatg agaaggcttt
     5641 tttcgctaac atcaacgatt ctcgcacgaa aaatttcagc ggtttgcaca atttctccgc
     5701 gaagattgga aggagcattc actttaatca gcgaaagttc acgatcaaca taactaatct
     5761 cagtaaaatc accaaccttg attacttcta ttaatttgta gagctgtttg gtgatttgtt
     5821 caagaatgtc ttcatcacca cgtaccacta aggtgattcg tgaaacatca gggtcctggg
     5881 tatgaccgac ggcaagactt tcgatattgt atccccgcct ggcgaaaagg gacgccactc
     5941 gtgccagaac ccgtggttta ttttccacca gaaccgatat tatatgttgc atggcacatc
     6001 tccttatcaa aattattcta aaatcatttc atctaatgat tttcccggag gcacaaaagg
     6061 atacacattc gtttctttac tgatacgaca atcaacaaga accggatgat ctttaacttc
     6121 cagaactctt ggtacggctc tgtcgagttc ctcttgattt tgaaccggga tgccttcagc
     6181 accaaaagct tgagccaggt tggcaaggtc aggattgata atatgagttg atgaatatcg
     6241 acaatcaaaa aagcattcct gccactgtct taccatgcca agataattat tgttcaaaac
     6301 gaaaatttta agaggaagac agttattgac cgcggtaacc agttcttgaa ttttcatcat
     6361 gatacttccg tcaccactaa atagaataac cgtctgatca ggattgccga tttgagcacc
     6421 aattgctgct ggaagaccaa atcccatagt tccaagacca cccgagctgg cccatctgcg
     6481 aggtcgatga cagagaaaat gttttgctgc ccacatttga tgttggccaa catcggtaac
     6541 caaaatggca tcaccctggg tagcttgata aaccgattcg atgatggtct gggctaccag
     6601 ttcattgttg gcgggtggtt ttggtgtata ctcttttttc catttttcaa tggtttctaa
     6661 ccactcatca tgttttttgg gttcggttct tttattgagc tcatttaaaa catttcgggc
     6721 atctccaaca atgggtatat taatcttgac atttttacca atttcagcag gatcaatatc
     6781 aatatgaacg atcttagccc gattggcaaa ggtggtggta tttccggtaa ctcgatcatc
     6841 aaagcgggca ccaacggcaa ttaaaagatc gcaattcata atagccttat tggcatggta
     6901 agaaccatgc attccaagcc atcctaatga aagtcgatga tcctcgggaa tggctccttt
     6961 tgccataagg gtataggtaa caggaatgtc ggttttttca acaacctcaa gaagaatatc
     7021 tgatgcccca gaagcaataa caccaccacc gacataaaag agaggccttg ttgcctcctt
     7081 tatggcctga gcggtcatgt tgatctggtt aaagtgacca cgataggtag gtttataacc
     7141 ggggatttct aatttttctg gatagttaaa atcaatctcg gcaatctgaa cgtcttttgg
     7201 aatatcgatt agg
//
LOCUS       DAOSOL010000033         7145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10515_length_7145_cov_4.044288, whole genome shotgun sequence.
ACCESSION   DAOSOL010000033 DAOSOL010000000
VERSION     DAOSOL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7145
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10515_length_7145_cov_4.044288"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PK171_01545"
     CDS             <1..542
                     /locus_tag="PK171_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOT04920.1"
                     /translation="GNCLLSYSCFDSLSSWLNTLKNWVIVYLGNFVGALLLVGLYHTS
                     GLFASGQGVIAERAYTIASAKVSLAFFPALSRGILCNWLVCFAVLLCIYSENNLTKLL
                     VIPAPILTFVALGYEHSVANMYFLPAGIIAQGYFSAEKLLHWGDIFRNLLPVTLGNII
                     GGVVLVSLFYWIIQGKLAK"
     gene            complement(669..887)
                     /locus_tag="PK171_01550"
     CDS             complement(669..887)
                     /locus_tag="PK171_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOT04921.1"
                     /translation="MREKVEKALEKVRGYLRMEGGDVELVDIVDGVVKVRLKGMCSSC
                     PMSMMTLKDGIERVVKEEVPEVVSVIQA"
     gene            complement(965..2221)
                     /gene="glyA"
                     /locus_tag="PK171_01555"
     CDS             complement(965..2221)
                     /gene="glyA"
                     /locus_tag="PK171_01555"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT04922.1"
                     /translation="MWENLKKSDPEIFHWVKEELNRQRNTLEMIASENFTSLAVLDAQ
                     GSVLTNKYAEGYPAKRYYGGCIFVDEVENIARQRALDIFGADHVNVQPHSGSQANMAV
                     YMAILKPGDTVLGMNLAHGGHLTHGSLVNFSGILYRFISYGVHPDTERINYDELEKLA
                     VEYKPKLIVAGASAYPRFIDFERLGKISGQIGAYLMVDMAHIAGLVAASVHPSPIPYA
                     DFVTTTTHKTLRGPRGGMIFCRKDFAGTIDKAVFPGTQGGPLLHVIAGKAVALKEAQS
                     EDFKVYQRQTVKNAAALAVSLKEKGFRLVSDGTDNHLILIDVRNQGITGKEAEKYLDQ
                     VGITVNKNAIPFDPEKPNTTSGIRIGTPSCTTRGMKEKEMSVIAKLICRTFESKNDIL
                     ALEKITHDVNQLCQQFPLYPDLIIPE"
     gene            complement(2233..2748)
                     /locus_tag="PK171_01560"
     CDS             complement(2233..2748)
                     /locus_tag="PK171_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOT04923.1"
                     /translation="MILKGKAHIFGDDINTDYIISGKYKFKTLDMNELGKHVMEDIDP
                     QFSQKVEIGDFIVAGKNFGCGSSREQAPRALLASGIRGVIAPSIARIFFRNAINCGLV
                     ALECDTSRIQSGDELEINLTTGKINDLTQNFTIEAAPLPEVMIQILNEGGLVSYFKKH
                     GSFPEPGKSQS"
     gene            complement(2754..4010)
                     /locus_tag="PK171_01565"
     CDS             complement(2754..4010)
                     /locus_tag="PK171_01565"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOT04924.1"
                     /translation="MGKTIAEKVFERKTGFPVKAGDLVIARIDMAMGQDGTTPLAIQS
                     FEEMGGKKVFDPSRIVFIIDHNAPSQLEAISQLHDNMRKFAENYGLTIYEVGCGVCHE
                     IMVAKGLVVPGDLVIGADSHTCTYGAIGAFSTGVGSTELAAAMISGQLWFKVPETILI
                     RINGTLPPGVYSKDVILYLASQIGADGATYQAIEFTGPIIDRLSVEERLTISNMAVEL
                     GAKAGIIAPDEKTLTWVKQRTIKSFEPIYPDPDAVYLKELTYDCSTLTPQVALPHRVD
                     TVTSIDSLGEVIIQEAYLGTCTNGRSVDIEVAAKILQDKKVHPKVRLIVAPASKEILL
                     EAIEKGWIETIVKAGGVLVTPGCGPCVGTHQGVPGDGWNVVSTANRNFKGRMGNNKAF
                     IYLASPATVAASALKGKITDPRKYLR"
     gene            4229..4960
                     /gene="nadE"
                     /locus_tag="PK171_01570"
     CDS             4229..4960
                     /gene="nadE"
                     /locus_tag="PK171_01570"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOT04925.1"
                     /translation="MKDWNQERDRIVDWLREQVQKAKAKGVVLGLSGGIDSSVAGALA
                     KIAFPENTLGLMLPCHSLPLDQQDAELIAGKFAISYQIFSLDFIYDNYLALLGESYSK
                     PLPLPLANIIPRIRMTVLYYFAQKENYLVCGATNKSELTVGYYTKYGDGGVDLMPLAH
                     LTKTEVWQFAKFLEIPASIIEKAPSAGLWEDQTDEKEMGICYAVLDAFIQNGLGNQQD
                     IAKIQKMIKRTEHKRQLPPRLERHS"
     gene            complement(5048..6787)
                     /gene="polX"
                     /locus_tag="PK171_01575"
     CDS             complement(5048..6787)
                     /gene="polX"
                     /locus_tag="PK171_01575"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HOT04926.1"
                     /translation="MRNLEVAQILREIGVLLEIKGENRFRVLAYQEAARKIENWPEAI
                     ELLAKEGKLRDISGIGEGLAAKIDEYLRTGKIEYYEELTKETPRELIQLTEIPGVGPK
                     IARQLYSDLGIVNIEQLEQAIQEHRLQNLPGFGIKSEEKIRKGIDIIKKSTGRMLLGY
                     ALPLAEEVIAFLRENNQIDNISTAGSLRRMKETIGDIDILASTADAERMMETFTKLPM
                     VKQVIAHGTTKSSILTYEGVQIDLRVVDNQCFGAALQYFTGSKEHNVKLREYAQKKGY
                     KINEYGIYRIDDEEKKGGEKEEEIYQILGMEWIPPEMREDQGEIEAALQKRLPVLVDI
                     KDIQGDLHVHSNWSDGLVSIEEMALAATKKGYQYLAICDHTQGLAVASGLTPEQIQER
                     RKEINQWNQNHSEIFVLEGVEANILGDGSIDLPDEVLAQLPIVVAGIHTGLSQTTEKI
                     NQRLEKAFKNPYVQIISHPTGRLINKRDATQGDLALLFKYAQQTTTALEINAQPERLD
                     LKDIDARQANEKYQIKLVISTDSHDPSSLNLMRLGVAQARRAWLTNKDILNTYNKGQL
                     LEILQKKRERFTI"
BASE COUNT         2050 a         1440 c         1343 g         2312 t
ORIGIN      
        1 caggaaattg ccttctttct tattcctgct ttgattcact ttcttcttgg ttgaatacat
       61 taaaaaactg ggtgattgtt tatttaggta attttgttgg agctcttctt ttggttggtc
      121 tttaccatac ttccggatta ttcgcttccg gacaaggggt catagctgaa agagcctata
      181 ctattgccag cgctaaggtg agtcttgctt tttttcccgc cttgagtcgt ggaattttat
      241 gcaactggtt agtttgcttt gctgtcttgc tttgtattta ttcagaaaat aatctgacca
      301 agctgctagt tattccggcc ccaattctaa cctttgtagc actgggttat gaacatagtg
      361 tagccaatat gtatttttta ccagccggaa tcatcgccca aggatatttt tctgcagaaa
      421 aactcttgca ctggggggat attttccgaa acctcttacc ggttacttta ggaaatatca
      481 ttggtggagt cgtcttggta agtctttttt attggattat tcagggaaag ttggcgaagt
      541 aagtagaaaa ttgtattgaa tggcaagcat tatttaatca aaaataaatt ttggaatgag
      601 tagtttaaaa ataaaaaaag cgtctgattt tcatgtcaga cgcttttttt atgagataat
      661 ctgattgatt aagcttgaat aaccgataca acctcgggaa cttcttcttt taccactcgc
      721 tcgataccgt ctttaagagt catcatcgac atcggacaag aactgcacat tccttttaat
      781 cggaccttca caaccccatc aacaatatct actaattcaa catctccacc ttccatacga
      841 agatatcctc tcaccttttc taaggccttt tctacttttt ctctcatcag tataaacctc
      901 ctcactggcg aatcctggaa ttaatttcct tgaccattac ttttaaaacc aaaatttaaa
      961 ttatctattc cggaatgatt agatcaggat agagtggaaa ttgttgacaa agctgattta
     1021 catcatgagt aattttttca agtgccagta tatcattttt tgattcgaat gtcctacaaa
     1081 tcagcttagc aatgaccgac atttcttttt ctttcatgcc acgagtcgta caggatggag
     1141 ttccaatccg aataccactg gtggtatttg gtttttcagg atcaaaggga atcgcatttt
     1201 tatttactgt tatacctact tggtccagat atttttcggc ttccttccct gtaatacctt
     1261 ggtttcgaac atcaatgaga atgagatgat tgtcagttcc atcggaaacc aagcgaaaac
     1321 ctttttcttt taaagagaca gcgagagctg ctgcattttt aaccgtttgt ctttgataga
     1381 ctttaaaatc ctcactttgt gcttctttaa gggctacggc tttaccggca atgacgtgca
     1441 agagaggacc tccttgagtt ccaggaaaaa cagctttatc aatagttcca gcaaaatctt
     1501 ttcggcaaaa aatcattcct ccacgaggtc cacgaagggt tttatgggtc gttgtggtta
     1561 caaaatcagc gtagggaata ggactgggat gaacactggc tgccaccaga cctgctatgt
     1621 gagccatatc caccatcaga tatgcaccta tttgtccgct gattttccct aacctttcaa
     1681 aatcgataaa gcgaggataa gcactggctc cagctactat caatttaggc ttatactcaa
     1741 cagccaattt ttctagttcg tcataattaa ttctttcggt atcaggatga accccatagg
     1801 aaatgaatcg atacaggatc ccagaaaaat tcaccaaact tccatgggtg agatgtcctc
     1861 catgagcaag attcattcca agtacagtat ccccgggttt gagaatggcc atataaacag
     1921 ccatattcgc ttgagaaccc gagtgaggtt gaacgttgac atgatcagct ccgaaaatat
     1981 ccaaagctct ttggcgagcg atattttcca cttcatcgac aaaaatgcaa ccgccgtaat
     2041 agcgtttagc cggatatcct tcagcgtatt tattagtgag caccgaaccc tgggcatcaa
     2101 gaacggctaa actggtaaaa ttttctgaag cgatcatttc caaggtattt ctttgacgat
     2161 taagttcttc ttttacccaa tggaatattt cgggatcgct cttttttaag ttttcccaca
     2221 taatattctc ctttaagatt ggcttttacc tggttcagga aatgatccat gttttttaaa
     2281 ataagaaacc aagccacctt catttaggat ttgaatcatt acttcaggaa gtggagccgc
     2341 ctcaatggta aagttttgag tcaagtcatt aatttttcct gttgttaagt taatttccag
     2401 ttcatcacca ctctgaatgc gactggtatc acactcgagt gccactaaac cacaattaat
     2461 agcattacga aagaatatac gggctattga tggagcaata actcctctta taccactggc
     2521 aagcaaagct ctgggagcct gttcacggga agagccacag ccaaaattct tcccggctac
     2581 aataaaatcg ccaatttcta ctttttgaga aaactgagga tcgatatctt ccatgacgtg
     2641 ttttcccagt tcgttcatat ccagagtttt aaatttgtat ttcccggaaa tgatatagtc
     2701 ggtgttaata tcatcgccga aaatgtgggc ttttcctttg agtatcatgg caatcacctc
     2761 aagtattttc tcggatcggt tatttttcct tttaaagccg aggcggcaac ggtagcggga
     2821 gaagctaagt atatgaaagc tttattgttt cccatgcgtc ctttaaaatt tcgatttgct
     2881 gtggaaacaa cattccatcc atcgcctggg actccttgat gagttccaac acaaggtcca
     2941 catccgggag ttaccagaac tcctccagct ttgacaatgg tttctatcca acctttctcg
     3001 attgcttcca aaagaatttc ttttgaggcc ggagcaacga taagacgaac ctttgggtgg
     3061 acttttttat cttgaagtat cttagcagca acctcaatgt ccacgcttcg accattggtg
     3121 caagttccta aataagcttc ttggattatc acctctccca aggaatcgat tgaggtaacc
     3181 gtatcaaccc gatgaggaag agcaacctgg ggagtaagcg ttgagcagtc ataggttaat
     3241 tcttttaaat aaacagcatc tgggtctgga taaattggtt caaaagactt aattgttctt
     3301 tgtttaaccc aggttaaggt tttttcatca ggagctataa tgcctgcttt ggctcctaat
     3361 tcaactgcca tgtttgagat agttaatcgt tcttcaaccg aaagacgatc aatgattgga
     3421 ccagtaaatt caatagcttg ataagttgct ccatcagcac caatttgaga ggcaagatac
     3481 agaatgacat ctttggaata taccccgggt gggagagttc cattgattcg aatcaggatg
     3541 gtttcaggaa ccttgaacca aagttgacct gatatcattg ctgcggctaa ttcagtactc
     3601 cctactccag ttgagaaggc tccaatggct ccataggtac aggtatgtga atcagctcca
     3661 atcactaaat caccagggac gactaaacct ttggcgacca tgatttcatg gcatactcca
     3721 catccaactt cataaatagt caatccatag ttttcagcaa acttccgcat gttatcatgg
     3781 agctgcgata tcgcttctag ttggcttggg gcattatggt caatgataaa tactatacga
     3841 gatgggtcga atactttttt accacccatt tcttcaaaag actggattgc cagtggtgtg
     3901 gtcccatctt gtcccatcgc catatcaatt cgggcaataa ctaaatctcc agccttaact
     3961 ggaaacccgg tttttcgttc aaaaaccttt tctgcaatgg tttttcccat atccgtcgct
     4021 cctttgagta tttgtccttc atctttttaa caataatcca aatttaatgg ataaaaactc
     4081 aactactatt ttgttctagt cggttcaaag ttaaaataat tgtatattga tttataatta
     4141 attatagtgc gacatgctaa ttttattcga tatatgtcta agaatgatac agtatcagag
     4201 aaataaatgt caatgaagga gaaatgatat gaaagattgg aatcaggaac gagatcgaat
     4261 agttgattgg ttaagggagc aggttcaaaa agcaaaagca aaaggagtgg ttcttggatt
     4321 aagtggagga attgattctt cggtagcggg agcattggct aaaattgcat ttccagaaaa
     4381 taccttaggt cttatgcttc cttgtcacag ccttcccctg gatcaacaag atgctgaact
     4441 aatagccgga aaatttgcta tttcatatca aattttttct cttgatttta tatatgataa
     4501 ctatctcgct ctccttggtg aaagctactc aaaacctctt cctcttccct tagctaatat
     4561 tattcctcgc atccgaatga cggtccttta ttattttgct caaaaggaaa attatctggt
     4621 ttgtggagca accaacaaaa gtgaattaac agttggatat tataccaaat atggggatgg
     4681 gggggttgac ctgatgcctc ttgcccattt gactaaaacg gaagtttggc aattcgcaaa
     4741 gtttttagaa ataccagcat ccataatcga aaaggcacca tcggcagggc tatgggaaga
     4801 tcaaactgat gaaaaggaaa tgggtatttg ttatgcagtt ttagatgcat ttattcaaaa
     4861 tgggttagga aatcaacagg atattgccaa aattcaaaaa atgataaagc ggactgaaca
     4921 caaacgacaa ctcccaccac gtttggaaag gcatagttga gtagtatgaa acgaataggc
     4981 ctttgcaata atggaaaaag attaatattt tcattgtttc tgaggttatg gaggagtagg
     5041 aatagatcta aattgtaaag cgttctctct ttttttggag gatttcaagg agttggcctt
     5101 tattgtaagt attgagaatg tccttattag tcaaccaagc tcgacgggct tgggcaacac
     5161 caagacgcat aagattcaat gaggaaggat cgtgagaatc agtagaaata accaatttaa
     5221 tctggtactt ttcgttggct tgtcgggcat caatatcttt aagatctaac cgttctggtt
     5281 gggcattgat ttcaagagcg gttgttgtct gttgagcgta tttaaaaaga agcgcgaggt
     5341 caccttgagt tgcatcccgt ttattgatca atcgtccggt ggggtgacta ataatctgaa
     5401 catagggatt tttaaaagcc ttttcaaggc gttggtttat tttttcagtg gtttgactta
     5461 atccggtgtg aattccagca actactatgg gaagttgagc aagcacttca tcaggaagat
     5521 caattgatcc atcacctaaa atattagctt ctaccccctc tagaacaaaa atctcactat
     5581 gattttggtt ccattggtta atctctttcc ttctttcttg tatttgctcg ggggttaaac
     5641 cagaagccac tgccaatccc tgggtatgat cacaaatagc gaggtattga tatccttttt
     5701 tagtagcagc taatgccatc tcttcaatgg aaaccaatcc atcactccaa tttgaatgga
     5761 cgtgaagatc tccttgaata tcttttatgt cgaccaatac cggtaagcgt ttttgaagag
     5821 cagcttcaat ttcaccttga tcttctctca tctccggtgg aatccactcc attcctaaaa
     5881 tttgataaat ttcttcttcc ttttctcccc ccttcttttc ctcatcatct atccgataaa
     5941 ttccatactc gttaatctta tacccttttt tttgtgcata ttcccgaagt ttaacgttat
     6001 gttctttaga accagtaaaa tactggagtg cggcgccaaa acattgatta tcaaccactc
     6061 gaagatcaat ttgtactccc tcataggtca agatactcga tttagttgtt ccatgggcaa
     6121 tgacttgctt caccataggt agtttggtaa aggtttccat catacgttct gcatcagccg
     6181 ttgatgccaa aatatcaata tcaccaatgg tttctttcat acgacgaagg cttccagctg
     6241 tgcttatgtt atcaatttga ttattttcac gaaggaaggc aataacttct tcagccaagg
     6301 gaagtgcgta acctaataac atcctaccgg tactcttctt gataatatcg atcccttttc
     6361 ttattttttc ttcacttttg ataccaaatc cagggagatt ttgaagacga tgttcctgaa
     6421 tggcttgttc taattgctca atgtttacaa tccctaagtc ggaatagagt tgtcgggcaa
     6481 tttttgggcc aaccccaggg atttcagtga gctgaatgag ttcccgaggg gtttctttag
     6541 taagttcttc ataatattcg attttcccgg tgcgaagata ttcatcgatc ttggctgcta
     6601 agccttcacc aatgcctgat atgtctcgta attttccttc tttggcgagt aattcaatcg
     6661 cttccggcca gttttcgatt tttctggcag cttcttgata ggccaatact cgaaaccggt
     6721 tttctccttt tatttctaag agaactccaa tttcacgaag aatttgagca acttccaaat
     6781 ttctcatagt cttatccctt aaggtaaaaa atatcttatt ttaattttat ttattcagtc
     6841 atgggttata aattcaaata ttgaatggaa tgattgcaat tcatatcaat tttttaaaaa
     6901 aagcttgaat aaacacttat aacattatgt catattctat gaatataata accaattttg
     6961 aattaattta atttcttatt ttttcaaaga cgagttagaa agaactgaaa aaaaatcgat
     7021 tccctcttat tctcccttta gtcaaagaga taaaaaaatt ttaaaaaaga tgagatcctc
     7081 acgccctcga aaaacgaggg ctcaggatga ccgattaaag aattcatttg atggtatatt
     7141 cagga
//
LOCUS       DAOSOL010000034         7116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10571_length_7116_cov_4.367795, whole genome shotgun sequence.
ACCESSION   DAOSOL010000034 DAOSOL010000000
VERSION     DAOSOL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10571_length_7116_cov_4.367795"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PK171_01580"
     CDS             complement(<1..266)
                     /locus_tag="PK171_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04927.1"
                     /translation="MKRAWCPGSMGELVQGTWEGKEVLISLTIDRFSEIEVDLVERVD
                     PKSREHMQELWKSKRAMELILQEWGLSRIAEKIVFRRCHSIPEG"
     gene            complement(273..1034)
                     /gene="cobS"
                     /locus_tag="PK171_01585"
     CDS             complement(273..1034)
                     /gene="cobS"
                     /locus_tag="PK171_01585"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOT04928.1"
                     /translation="MESSRSAIKPKIWHYWFVAMSFLTCIPVPKFNYQNGDLSRAAVF
                     FPIVGGLVGAFSYGLYYLLRFVNAGNWLIALVLVATSLVLTRGLHLDGWADVCDALFS
                     TQDLKRRREILKDSHLGTFGVTGIFLLLSFKVIVLGGNFLPIYLIIAPMAGRTAALII
                     GGLFHPFRQEKKSLGEEFIGQIPRWVMGTWLGVIMLFPWFLGWHYYPFKAALIFFVGW
                     LFSKMMERGFRGLSGDAVGAIIELVETLFLYLGRI"
     gene            complement(1034..1579)
                     /gene="cobU"
                     /locus_tag="PK171_01590"
     CDS             complement(1034..1579)
                     /gene="cobU"
                     /locus_tag="PK171_01590"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOT04929.1"
                     /translation="MPEPYKKTVLILGGARSGKSSLAHQMARDSGLPVIYLATGVVTD
                     MEMKERVQKHRNSRPQSWLTRETPYGFSHIDENWTGKAILLDCVTFLVNNLLLREDKE
                     EAAYHQLLNEFIYLFEKQQREGFYFLMVSNETGMGIVPEYPLARTFRDLQGRVNQWLA
                     TKVNQVYLVVAGIPVKIKGEI"
     gene            complement(1563..2570)
                     /locus_tag="PK171_01595"
     CDS             complement(1563..2570)
                     /locus_tag="PK171_01595"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT04930.1"
                     /translation="MDFSVNLNPWGPPQILKEHWNDWFRYVSYYPPLDWEFYQKRIAL
                     LYNLDQNLILPFNGATQGIYFIARRHQAKKIFVIEPCFTEYARAFNLEGKTVIHSNLL
                     FREGFHSIMSRIERESPDLIVLGNPGNPLGQCLSKKERLILYHWCREKKVALLVDEAF
                     QEFIQEKTSFLPQLNGEDQTLIIIRSLTKYYSLAGLRGGFIVANPSLVEEWKMALEPW
                     SINTLLAVTLDLLASIDLGEFHSLTLKELRNEKRYIEESLGKLFNLFELQPSVVNFYT
                     IRIKAQTSCFYSFLQNQKLIVRFLGDFWGMSNDFFRFSVRTHEENIKLIQVINEYART
                     L"
     gene            complement(2630..5050)
                     /gene="gyrA"
                     /locus_tag="PK171_01600"
     CDS             complement(2630..5050)
                     /gene="gyrA"
                     /locus_tag="PK171_01600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT04931.1"
                     /translation="MPEDKGNIIPVDVKDEMKDAYLNYAMSVIVGRALPDARDGLKPV
                     QRRILFAMNEMGIRHNLPYKKSARIVGDVLGKYHPHGDMAIYDTLVRMAQPFTYRYSL
                     VDGHGNFGSIDGDPPAAMRYTEVRMNLIAEEMLLDIEKNTVDFIPNFDESMQEPIVLP
                     ANFPQLLANGATGIAVGMATNIPPHNLSEIIDATIYLIDHPQSTWRELMEYIPGPDFP
                     TYGLIVGTEGIHDYFREGRGKLILRGDARIEDLDRHRKAIIIREIPYQVNKASLVEEI
                     ARLIMDKKITDVSEVRDESDRDGIRVVLELKKNAMNPNFVLNYLYRRTALQTTFGVIL
                     LALINGRPEVMGVPDVLRHFIDYRKEVVTRRSKYELKNAEDRIHILEGFLRALDRIDE
                     VIHTIRSSQNVSEAKDRLIQQFQFSDPQAQAILEMRLQRLVALEREKIQEEYKELEKR
                     IASLKDILTHEKSLLNLIKRELLDVKRRFGDARRTRIVDEAEPLDLTDLIAEEDIVIT
                     LTRDGYVKRVLLSSYRRQGRGGRGVTGITTKEEDLVSQIAVTTTLHKILFFTSRGRVF
                     QLPAYQLVEMQRQAKGIHLVNLLQLEDGEQVINMIPLKDFGEGKYLFFVTSRGIVKKS
                     DLLEYVSITRRGIRAINLDDGDELSTVFVTNGKEKVLIATAKGYGISFSEEEVRSMGR
                     VSRGVKGITLRAKDRVVGASSLKHKLDLLIISSKGMGKRVKMQNFPVHHRGGKGIILM
                     KFGQPENEVIGVQLVSGQEEILISSQNGTLIRINCNEVSVQGRATRGVTLVRLYKGDL
                     ISSFALVE"
     gene            complement(5079..6995)
                     /gene="gyrB"
                     /locus_tag="PK171_01605"
     CDS             complement(5079..6995)
                     /gene="gyrB"
                     /locus_tag="PK171_01605"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOT04932.1"
                     /translation="MEEPRKNDIKNYGAEQIQVLEGLEAVRKRPSMYIGNTSVEGLHH
                     LVFEVVDNSVDEALMGYCDEIIVTIQKEGSVMVQDNGRGIPVGVHQKVGRSAVEVVLT
                     KLHAGGKFDRYSYKISGGLHGVGVSVVNALSEWLEVEIHQNQKIWRQRYERGKPINDL
                     VDAGLTNHTGTVIRFLPDPEIFPDRNFHFDILAQRLKELAFLNEGLKICLKDERNEKE
                     KEYCYHGGIKSLVSYLNRGKNVVHNDPIFLKGEKDDVKVEVAFQYNDSYLENLISFTN
                     NINTIEGGSHVSGFKLAINRSFSEYIERYQLIKEKEEMPTGNDIREGLCAVISVLVPE
                     PQFEGQTKTKLGNIEAKGIVEEVTYSYLSSFLEENPDVAKAICEKIIQAARAREAARK
                     ARELARKKNSIDSSSLPGKLADCSSRDPKESEIFIVEGDSAGGSAKQGRDRRFQAILP
                     LRGKILNVEKANLHKILSNAEIRNIVAALGCGIGFDFVIDKLRYYKVIIMTDADVDGA
                     HIRTLLLTFFYRYMKPLIENGKIYIALPPLYRIRDNKKDAYAYSDKELEKILKDFKNK
                     KYTIQRYKGLGEMNPEQLWETAMDPKTRTLKQVSIEDGIEAEEIFSILMGDAVDPRRE
                     FIREHAHEVVNIDI"
BASE COUNT         2054 a         1618 c         1206 g         2238 t
ORIGIN      
        1 tttccttctg gaattgagtg gcaccttcga aacacaattt tttccgctat tctactcaat
       61 ccccattctt gaagaattag ctccatggca cgtttgctct tccaaagctc ttgcatgtgt
      121 tccctgcttt ttggatcaac cctttcaacc aaatcaactt ctatttctga gaaacggtca
      181 atggtcaaag atatgagtac ttccttgcct tcccaggttc cttgaactaa ttctcccata
      241 ctacccggac accatgcgcg tttcattcct gtttatatcc tccctaaata taaaaaaagg
      301 gtttcaacaa gctctataat tgctcctaca gcatcaccac taagtccccg aaaacctctt
      361 tccatcattt tactgaataa ccagccaaca aaaaaaatca aggctgcttt gaaaggatag
      421 taatgccacc ccaaaaacca gggaaacagc attataaccc caagccaagt ccccattacc
      481 caccttggga tctgaccaat aaattcttct ccaagactct ttttctcttg acgaaaggga
      541 tggaaaagcc ctccaattat taaagcagcg gttcttcctg ccattggggc aatgattaaa
      601 taaattggta gaaaatttcc ccccagtact attaccttaa agcttaataa tagaaatatt
      661 ccagttacac caaaagttcc aagatgacta tctttgagaa tttctcgcct tcttttcagg
      721 tcctgagttg aaaaaagcgc atcacaaaca tcagcccatc catcaagatg caatcctcga
      781 gttaaaacta atgaggtagc gactaacacc aaagcgatta accaattccc agcgttgaca
      841 aaccgaagga gataatataa tccatagctg aatgctccta ctaaccctcc aacaataggg
      901 aaaaaaacag ctgctcggga aagatctccg ttttgataat taaattttgg gaccggaatg
      961 caagttaaaa aactcatggc aacaaaccaa taatgccaaa tctttggttt aatcgcggaa
     1021 cggctagatt ccattatatc tctcccttta tcttcactgg aatgccggca actactaagt
     1081 agacctggtt aactttagtt gccaaccatt ggttaacgcg accttggaga tcacgaaagg
     1141 ttctcgccag agggtattca ggcacaatcc ccattcccgt ctcattgctc accattaaaa
     1201 agtaaaaacc ctctcgttgt tgtttttcaa aaagataaat gaattcatta agaagttggt
     1261 gataggctgc ttcttctttg tcttctcgta ataacagatt gttaaccaga aatgtaacgc
     1321 aatccagcaa aattgccttt ccagtccagt tttcatcgat atgactgaaa ccatagggag
     1381 tctctctggt taaccacgat tgaggacgag aattccgatg cttttgaact ctttccttca
     1441 tttccatatc ggtaaccact ccggttgcta aataaattac tggtaagcca ctatctcgag
     1501 ccatttgatg cgcaagagaa ctttttccgc tccgggctcc tcccaagatc agaactgttt
     1561 ttttataagg ttcgggcata ttcatttatc acctgaatta attttatatt ctcttcgtga
     1621 gtccgaactg aaaaccggaa aaaatcatta gacattcccc aaaaatcacc caaaaagcgc
     1681 acaatgagct tttggttttg aaggaaagaa tagaagcaac ttgtctgggc tttgattctt
     1741 atcgtataaa aatttactac tgaaggttga agttcaaaaa ggttaaagag tttccctagg
     1801 ctctcttcaa tatatcgttt ttcattacgt agttccttca acgtaaggga atgaaattca
     1861 ccaagatcga tactggcaag caaatctaaa gttacagcca aaagcgtatt tatactccag
     1921 ggttcaagag ccattttcca ctcctcaacc aatgatgggt tagccactat aaagcctccc
     1981 ctcaatccag ctaaggaata atattttgta agagagcgga tgatgattaa ggtttggtcc
     2041 tcaccgttca attgtggtaa aaaagaagtt ttttcctgaa taaactcttg aaatgcttca
     2101 tcaaccaaca atgccacctt tttctctcga caccaatggt ataaaatgag cctttccttt
     2161 tttgaaagac attgacctaa gggattacca ggattcccta atacgattag atccgggctt
     2221 tccctttcta tacgagacat tatgctatgg aatccttctc ggaacaaaag attactatgg
     2281 attactgttt ttccttctaa attaaaagca cgagcatatt cagtaaaaca tggctctata
     2341 acaaagattt ttttagcctg atgacgacgg gcgataaaat aaataccctg ggttgcgcca
     2401 ttgaagggaa gaatcaggtt ttgatccaaa ttataaagca gggctatcct tttttgataa
     2461 aattcccaat caagaggggg ataataggaa acataccgaa accaatcatt ccagtgttcc
     2521 tttaatattt gaggcggacc ccacggattt aagttaacac tgaaatccac cggggatttg
     2581 cctctcaatt ccatctctat tagtcgacca ccatggatgg gtacgttcat cattcaacca
     2641 gtgcaaatga ggagattaaa tcacctttgt acaatcttac cagggttacg ccccgggttg
     2701 cacgtccttg aactgaaact tcattgcaat tgattcgaat tagggtgcca ttttgcgatg
     2761 atatcaatat ctcttcctgt ccagaaacta gctgaactcc aataacttcg ttttctggtt
     2821 ggccaaactt cattaagata atgcccttcc cgcctcggtg gtggacagga aagttctgca
     2881 tttttactcg cttacccatg cccttagagg aaataatcaa tagatctaat ttatgtttga
     2941 gagatgaagc accgacaacc cggtccttcg ctcgaagcgt tattcctttt acccctcgac
     3001 taactctccc cattgagcga acctcttcct cagaaaagga aattccataa cccttcgctg
     3061 tagcaatcag aactttttcc tttccattgg ttacaaatac tgttgagagc tcatcgccat
     3121 catcgagatt aattgctctt attcctcttc tggtgataga aacatactca aggagatcgc
     3181 tctttttaac gataccccgc gatgtaacaa aaaataagta ctttccttcg ccaaaatcct
     3241 tgaggggtat catattaatc acttgttctc cgtcttcgag ttggagtaaa tttactaaat
     3301 ggattccctt ggcttggcgc tgcatttcaa ccagttgata agcaggaagc tggaataccc
     3361 gtccccgact ggtaaaaaat agaattttgt gaagagtcgt tgtaaccgca atttgactaa
     3421 ccaaatcttc ctctttagtt gtaataccag ttacccctcg accgcctcgt ccttgacgcc
     3481 gataggaaga aaggagaact cttttgacat aaccatcacg agtaagggta ataacaatat
     3541 cctcttcagc aataagatcg gttaaatcaa gaggctccgc ttcatcaaca atacgggttc
     3601 gacgagcgtc gccaaacctt ctttttacat ctaaaagctc tctttttatt aagttcaaaa
     3661 ggctcttttc atgggttaat atatctttca gagaagcaat tcttttctct aattccttat
     3721 attcctcttg gatcttttcc ctttccaagg ccaccaaccg ttgaagtctc atttctaaaa
     3781 ttgcttgtgc ctgaggatcg gaaaactgaa attgctgaat taaacgatct tttgcttcgg
     3841 atacattttg agaagatcga atagtatgaa ttacctcgtc tatcctatct aatgcccgaa
     3901 ggaatccctc taaaatatga attctatctt cggcattttt caattcatac ttactacgcc
     3961 gagtcaccac ttcttttcga taatcgataa aatgacgaag aacatcaggc acgcccatta
     4021 cttccggtcg tccattaatc agcgctaaaa gaataactcc aaaggtagtt tgaagagcgg
     4081 ttcgacgata aaggtaattc aaaacaaagt ttggattcat ggcatttttt ttcagttcca
     4141 gaaccaccct aataccgtct cggtctgact catcacgaac ttcactaaca tcagtgattt
     4201 ttttgtccat aatgagtcga gcaatttcct caaccaacga agctttgttg acttgataag
     4261 gaatttcacg aattattatt gcttttctat gtcggtctaa atcttcaatt cgggcgtctc
     4321 cccgcaatat taattttcct cgtccttctc taaagtaatc atgaatacct tcagttccaa
     4381 ctattaatcc ataagtagga aaatccggtc cgggaatata ttccattaac tccctccaag
     4441 ttgactgagg gtgatcaata agatatatag tggcatcgat tatctccgaa aggttatgag
     4501 gaggaatgtt ggttgccatt ccaactgcta tgccggttgc accattggct aaaagttgag
     4561 gaaagtttgc tggtaaaact atcggttcct gcattgactc atcaaaattt ggtataaagt
     4621 cgacagtgtt tttttcgata tccaacaaca tttcttcggc aatcaggttc attcttactt
     4681 cagtatatct catggctgct ggtggatcgc catcgatcga tccaaaattt ccgtgcccat
     4741 caaccaaaga gtaacgatag gtaaaaggtt gggccattcg aactaaggta tcatatatag
     4801 ccatatcacc atgaggatga tactttccca gaacatcgcc aacaattcga gcgctttttt
     4861 tgtacggtaa attgtgccta atacccattt cattcatcgc aaacagaatt cggcgttgta
     4921 cgggtttcag cccatctcga gcatcaggca atgcccttcc gacaataacg ctcatggcat
     4981 aatttaaata agcatctttc atttcatctt ttacgtctac aggaataata ttccctttat
     5041 cttccggcat ttaattcctc caagattatc tctttgaatt atatgtctat gttaactact
     5101 tcatgagcat gctcacggat aaactcacga cgtggatcaa cagcatctcc cattaaaatg
     5161 ctaaatattt cctcagcttc aataccatcc tcaatagaaa cctgcttgag agttctagtt
     5221 ttgggatcca tcgctgtttc ccagagctgt tctggattca tttctcctaa gcctttatag
     5281 cgttgaatag tgtatttttt gtttttaaag tcttttaata ttttttctaa ttctttatca
     5341 ctataagcat aagcatcttt tttattatcc cttattcgat agagcggtgg aagggcaata
     5401 taaatttttc cattttctat gagtggcttc atgtatcggt aaaaaaaggt taacaataaa
     5461 gtacggatat gggcaccatc tacatcagca tcggtcataa ttatcacttt gtagtagcgt
     5521 aatttatcaa tgacaaagtc aaatccaatt ccacatccta aggcagcaac aatgttgcgg
     5581 atttcagcgt tggaaagaat tttatgaagg tttgccttct caacgttgag aattttcccc
     5641 cttaggggta gaattgcctg gaaacggcga tctcttcctt gttttgctga gcctcctgcc
     5701 gaatctccct caactataaa tatttccgat tccttgggat cccgacttga acaatccgct
     5761 aatttcccag ggagactgga tgagtcgatg ctgttctttt ttcgagctaa ttcgcgggct
     5821 ttacgagcag cttcacgggc ccttgccgct tgaattattt tttcgcatat agctttagcc
     5881 acatcaggat tttcttctaa gaaggaagaa agatagctat atgtaacttc ctccacaata
     5941 cctttagcct ctatgtttcc aagcttggtt ttagtctgcc cttcaaactg tggttcaggg
     6001 acaagaacac taattactgc acatagcccc tctcgaatgt cgtttccggt cggcatttcc
     6061 tctttttctt ttataagctg ataacgttct atatattctg agaatgatct atttatcgcc
     6121 aacttgaaac cgcttacgtg actaccacct tctatggtat taatattatt ggtaaaagaa
     6181 ataaggtttt ccaagtagct gtcgttatat tgaaaagcaa cctcaacttt tacatcatcc
     6241 ttttcacctt taagaaaaat tggatcgttg tgtacaacgt ttttacctcg gttcaaatag
     6301 ctaacaagag actttattcc accgtgataa caatactctt tttctttttc gttacgttca
     6361 tcttttaaac aaatttttag accttcattg agaaaggcta gttcttttaa cctttgagct
     6421 aaaatatcaa agtgaaaatt gcgatctggg aatatttcgg ggtcgggaag aaagcgaatt
     6481 accgttcctg tgtggttagt aagaccggca tcgaccaaat catttatcgg tttaccccgc
     6541 tcataccttt gtcgccaaat tttttggttt tggtggattt ctacctctaa ccattctgat
     6601 aaagcattta caacggatac tcccaccccg tggagaccgc cggatatttt ataggagtat
     6661 cgatcaaact tccctccagc atggagtttg gtgaggacta cctctactgc tgaacggcca
     6721 accttttgat gaactccaac tggaattcct ctcccattat cctgcaccat cacactgcct
     6781 tctttttgaa ttgttacaat tatctcatca caatatccca ttaatgcttc atccacacta
     6841 ttgtcaacaa cttcaaacac aagatggtgg aggccttcta ctgatgtatt cccaatatac
     6901 atggatggtc gttttctaac tgcctctaaa ccttctaaaa cctgtatctg ctctgctcca
     6961 tagtttttta tatcattttt tcttggttct tccaaaatct ttcacctcat aaatcattgg
     7021 tattatagat cagtcgtctt ttcgataaag ggaaaataaa tttaattttt ctattttttc
     7081 cctataccac ggatttttac tttttttacc attaat
//
LOCUS       DAOSOL010000035         7022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10771_length_7022_cov_4.254055, whole genome shotgun sequence.
ACCESSION   DAOSOL010000035 DAOSOL010000000
VERSION     DAOSOL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10771_length_7022_cov_4.254055"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..996)
                     /gene="aroE"
                     /locus_tag="PK171_01610"
     CDS             complement(130..996)
                     /gene="aroE"
                     /locus_tag="PK171_01610"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT04933.1"
                     /translation="MVQQNRITARTNLLGLIGHPVDHSLSPVFQNAALNALNLDYVYL
                     AFDISTGDLMDAVRTLRTWRLKGINVTVPHKEKIIQWLDRLDDTALLLGAVNVVVVKD
                     EELVGYNTDGIGFAKALDFNQINLNGKQVALLGAGGAARAVLSVLIERGVRKVIVFNR
                     SFERSLQFQQWAKNSFTTVIEIDNWESFINGQSSFLSQVDVLINATSLGLSQEIIEVP
                     WNGIDNCELVIDVVYNREETPLVREARKKGKKAFDGKMMLLFQGAESFRLFTGFEAPI
                     GVMEKALNEALR"
     gene            complement(975..1493)
                     /locus_tag="PK171_01615"
     CDS             complement(975..1493)
                     /locus_tag="PK171_01615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HOT04934.1"
                     /translation="MKQLLRKFYPSLYVDEISEIPLDLLKKRGIQGLIIDLDNTIAPW
                     DQPTVTQSAERWLKKAKNDGFKIFLVSNSTTSRVNYFMESLGIPGISMAQKPRRGSFR
                     KALESMDLNYNQVTVIGDQIFTDVLGGNRLNLHTILVNPINRKEFFFTRLVRLVEKFV
                     MKRFLQWFNKTE"
     gene            complement(1490..2473)
                     /gene="mltG"
                     /locus_tag="PK171_01620"
     CDS             complement(1490..2473)
                     /gene="mltG"
                     /locus_tag="PK171_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT04935.1"
                     /translation="MKKYHFGLIVVTYLFCFILYISFYYSPGLSGVSQTMVIQSGMTN
                     RVIAQDFKKNDLISSPALFLAFTFLSDQSRLIAGNYQFRSGETISQIVSRIAQGRSYQ
                     VKVTFPEGSTVKQMAEWLDQAGICRQEDYLSYTQQPTIFQKPWLSEAKNLEGYLFPDT
                     YYLSPGTNPKQVIEIQLKRFEEVYPGSLLEESFTEMSQKVILASIVEREAQLINEKPI
                     VASVFLNRLKSNMKLESCATVIYAWNQEKGVQLSSLSLEDLTIPSPYNTYLHKGLPPT
                     PICNPSLDSLEAVVNPPQTDYYFFVLGENGSHNFSKTFDEHLKNKQKSTIQ"
     gene            complement(2470..2883)
                     /gene="ruvX"
                     /locus_tag="PK171_01625"
     CDS             complement(2470..2883)
                     /gene="ruvX"
                     /locus_tag="PK171_01625"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOT04936.1"
                     /translation="MKKILAVDLGEKRIGFSYNRGTSFASPLKVISSENLKKNVENII
                     EVAQQVQAEVIVLGLPLRLNNSVKSEAQKIIEFRELLIKRFPGEVVLFDERLTTKEAE
                     KRLLEADVSRKKRKMVIDQLAATIILQTYIDSTQI"
     gene            complement(2977..5589)
                     /gene="alaS"
                     /locus_tag="PK171_01630"
     CDS             complement(2977..5589)
                     /gene="alaS"
                     /locus_tag="PK171_01630"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT04937.1"
                     /translation="MTSSELRQKFLDFFEKRGHKILPSASLIPNDPTLLLTIAGMVPF
                     KPIFLNKIQPNFKRVVSVQKCVRVNDLGNVGHTARHHTFFEMLGNFSFGDYFKKEACL
                     WGWEFLTQTIGFSPDRMWVSVHRKDQEAFEVWKNCVGVPPERIVFLGDEDNFWASGPI
                     GPCGYCSEIYYDRGEVKGCGQSTCAPGCNCDRYLEVWNLVFMEFDRKADGTLEELPKK
                     NIDTGMGLERLASVVQETDTDYETDLFFPIIQGLEKISGNDYLIPALKPPFRIIADHI
                     RAIVFLITDGIYPSNEGRGYILRRLIRRAYRYGGKIGIHQPFLHELLFTVIDGMSNAY
                     PELFMRKDIISKIVFQEESRFQGTLQSGEAFLSQLIEQTKKNKKQQLSGKDIFMLYDT
                     YGFPSDVAEDILQEEGLQFDKTEFENQMEQQKNRAREARGEKDRLIRSQKEVEELFTG
                     VYSRFVGYNQSMVNTDMVALSDGEDRISQAETGQKVIVALKESPFYPEKGGQEFDTGW
                     IESEQGRVQVEKVYSEFLIEGTVVSGQIKENDRVWAKIDTQRRRLIEIHHTTTHLLHR
                     ALREILGSQVKQAGSWVGESGLRFDFTHFSALSEDEIDAVEEIVNQKIFEDLPVVISY
                     SSLDEAQRAGVIALFEEKYQSLVRIVRVGDYTAELCGGTHLRRTSEAGLFKIITESSI
                     GSGLRRVEAVAGSVAYKYLQDYFHLGRDIKKIFGSDHYQILPAINNLIENNKHYKEEL
                     QRTFQSHARQAILDALQVAGSNNPPIYLTHLFVENPPEIDYLKEMVDELRTRLSQGIV
                     VLGIKKDEAISGVLAEINLPQPIDLNRLVREITKKIGGGGGGRPSLVQFGGIPFGSWE
                     KFVEEIKKMTNQIS"
     gene            complement(5644..6624)
                     /locus_tag="PK171_01635"
     CDS             complement(5644..6624)
                     /locus_tag="PK171_01635"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04938.1"
                     /translation="MIEVVDVVFEKRIQNVINEIKDIAEGFERVVVAFSGGVDSTVAL
                     FLSIYALGREKVQACTVDWEEYFPYHARENIDFLISCFHVPHLYLPGRNIIEEVVSRG
                     PACNLCTRKAKLDNLQKNFGEKTLIIGGANQSDSWGIRGLKLIRNTYSPLFDFSKQEI
                     FQICQIMNIPVRRIGENSVREGCVLKHLYKPLAAKVHGQAVVKANQVLLQTLHQEGFK
                     SEIANVKIVGPLQKNQALINLRPFPNPKLKSIISQRMTDFEEIDTITWVDRPLTLVIR
                     ANPGLYRNFDSRYWIENGKIQPDFVQPVSFQWMPSTNRRLRTFQVVDFWI"
     gene            complement(6621..>7022)
                     /locus_tag="PK171_01640"
     CDS             complement(6621..>7022)
                     /locus_tag="PK171_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT04939.1"
                     /translation="PWFEIKNKYPVGSIHTGKVVRIVDFGAFIELEKGWDGLVHISEI
                     SDKRISSPSEVIEENQEVNVKIIKLDDQERKIGLSIKQTLPRDEEKERTKEREKPKKE
                     QPITQTNGRTTLGDVFGDTFNNLLNNMKNNS"
BASE COUNT         2088 a         1528 c         1206 g         2200 t
ORIGIN      
        1 gatgagattc tcacgtcgtc cagaaaaaaa caccagactc ctcagaatga cggagaagcc
       61 ggatgagatt gctacgtcat ccgatagaaa caccggactc ttcagaatga cagaaagtca
      121 gcaagatact tacctcaatg cctcattcaa ggctttttcc attactccaa tgggggcttc
      181 aaagccagta aacaaacgaa aactctccgc tccctgaaaa agtaacatca tctttccatc
      241 aaaagctttt ttcccctttt ttcgtgcttc tcttaccaga ggagtttctt cacgattata
      301 gactacatca attaccaatt cacagttatc aataccgttc catggaactt caatgatttc
      361 ctggctaagt cccaaactag tagcattaat caaaacatca acctggctca aaaaagaaga
      421 ttgaccattt ataaagctct cccagttatc aatttctatt accgtggtga atgaattttt
      481 tgcccactgc tgaaattgta aagacctctc gaacgaacga ttaaacacta taaccttacg
      541 aactcctctt tcaatgagca ccgataaaac agctcgagca gctcctccag ctcccaaaag
      601 agcaacctgt ttaccattaa ggttaatctg attaaaatcc agagctttag cgaaaccaat
      661 cccatcagta ttataaccaa ctaactcttc atcttttaca accacaacat tcacagctcc
      721 caatagtaag gcagtatcat ccaatctatc caaccattga ataatttttt ccttgtgtgg
      781 aactgtaacg tttattccct tcaatcgcca agttcgaagc gtccgaacag cgtccataag
      841 gtccccagtg gaaatatcga aagctaaata gacgtagtcg aggtttaaag cgttgagcgc
      901 tgcattttga aatactggtg acagtgaatg atccactgga tgtccaatga gtcctaacaa
      961 gttggttcga gcggttattc ggttttgttg aaccattgaa gaaacctttt cataacaaat
     1021 ttttccacca atcgtaccag gcgggtaaaa aagaattctt ttcggttaat gggattgact
     1081 aaaatagtat ggagatttaa tcgattccct cctaaaacat cggtaaatat ttgatcacca
     1141 atcacagtaa cctgattgta attcaagtcc atcgattcca gagccttacg aaaggaaccg
     1201 cgtcgcggct tttgggccat agatatccca gggataccca aggattccat gaagtaattg
     1261 actcttgaag ttgtggaatt ggacaccaga aatattttaa atccatcatt tttagctttt
     1321 ttcagccacc tttctgccga ctgagtaaca gttggctggt cccatggtgc gatagtgtta
     1381 tcgagatcga taatcaaacc ctgtatccct cttttcttta acaaatccag tgggatctca
     1441 gatatttcat caacataaag tgatggataa aatttcctta acaactgctt cattggattg
     1501 tactcttctg tttgtttttt aggtgttcat caaaggtttt ggaaaaattg tgcgatccat
     1561 tttctcccag tacaaaaaaa taataatcgg tttgaggagg atttactact gcttctaaag
     1621 aatcaagact aggattacaa atcggagttg gaggaagccc tttgtgaagg taggtattgt
     1681 aaggagatgg tatggttaaa tcttccaagc tcaatgatga gagctgcact cccttttcct
     1741 gattccaggc ataaatgacc gttgcacacg actctaattt catattcgat ttcaagcgat
     1801 ttaaaaaaac tgaagcgact ataggttttt catttattaa ctgtgcttct ctctcgacta
     1861 ttgaagctaa tattactttt tggctcatct cggtaaaact ttcctccaag agacttccgg
     1921 gataaacttc ctcaaatcgt tttaattgga tttcaatgac ctgcttggga ttggtgcctg
     1981 gtgataaata ataagtatcg ggaaacaaat aaccttctaa attttttgct tcggataacc
     2041 atggtttttg aaaaattgtc ggttgttgag tataacttaa ataatcctcc tgtcgacaaa
     2101 ttccagcttg atcaagccac tcagccattt gtttaactgt tgaaccctca ggaaaggtta
     2161 ctttgacttg ataagaacga ccttgagcaa ttctgctcac aatttgggaa atggtttctc
     2221 ctgatcgaaa ctgataattc ccagcaatca atcgactttg atcgcttaaa aatgtaaaag
     2281 ccaaaaaaag agctggagaa gatatgagat cgttcttctt aaaatcttgg gcaatcactc
     2341 gattggtcat tccggattgg ataaccatgg tttgagaaac accacttaat cctggagaat
     2401 aataaaatga tatataaaga ataaaacaaa ataaataagt aacaacgatt aatccgaaat
     2461 gatatttttt catatttgag tagaatcaat ataggtttgg agaattatgg ttgccgctaa
     2521 ctgatcgata accattttcc tttttttccg acttacatca gcttccagca atcttttctc
     2581 tgcttctttg gtcgttaacc tctcgtcaaa aagtaccact tcaccgggaa aacgcttaat
     2641 taataactca cgaaactcga taattttttg cgcttcgctt tttactgaat tatttagtcg
     2701 caaaggcaaa cccaaaacaa tgacttcagc ttgaacttgt tgggccactt cgattatatt
     2761 ctcaacattt ttttttaggt tctcactgga aatcaccttc aaaggtgaag caaagcttgt
     2821 cccacgattg taagaaaacc cgattctctt ttctcctaaa tcgaccgcta atattttttt
     2881 caccaacaaa ttcctttgag tttttgatat tgataaatca agttttactg aatttataat
     2941 ataaaaagct tatcttttcc ctcttcttct tcaatatcaa gagatttgat tggtcatttt
     3001 cttgatttct tctacaaatt tttcccaaga gccaaagggg ataccaccaa attgaaccaa
     3061 agacggcctc cctcctccac caccaccaat tttcttggtt atttcacgaa ccaaacgatt
     3121 caaatcgatt ggctggggga gatttatttc agctaaaaca ccagaaattg cttcatcttt
     3181 tttaatcccc aagactacta ttccctggct caggcgagta cgtaattcat caaccatctc
     3241 ttttaaatag tctatctccg gtggattttc aacaaaaaga tgagtcaggt aaattggcgg
     3301 gttgttgctt ccagcaactt ggagagcatc aagaattgcc tgacgtgcat gagattgaaa
     3361 ggtccgttgg agttcttctt tataatgttt attattctcg attaaattat taatagctgg
     3421 taatatctga taatgatccg atccaaaaat ttttttaata tcacgtccca ggtggaaata
     3481 gtcctgaaga tatttataag caactgatcc agctactgct tctactcttc ttaaacccga
     3541 ccctatgctg gattcggtaa taattttaaa aagaccagcc tcactggttc gccgtaaatg
     3601 ggttcctcca caaagctcgg cagtgtaatc acccactcgg acaatccgaa ctagtgattg
     3661 atatttctct tcaaacaatg caataacacc tgctctctga gcttcatcca ggcttgaata
     3721 acttatcacc accggtaagt cctcaaaaat cttttgattt acaatctctt ctacagcgtc
     3781 gatttcatcc tcagacaaag cagaaaaatg ggtaaaatca aagcgaagtc cactttctcc
     3841 tacccaggat ccagcttgtt ttacttgtga acccaagatt tcccgcagtg ctcgatgaag
     3901 caagtgagtg gtcgtgtgat gaatttcaat gagtcggcga cgttgggtat caatttttgc
     3961 ccaaactcga tcattttctt tgatttgtcc actgactact gtaccctcga ttaaaaattc
     4021 agagtagacc ttctcgacct gaacccttcc ttgttcggat tctatccagc cggtatcaaa
     4081 ctcttgccct cctttttccg gataaaaggg tgattctttc aaggcgacga ttaccttctg
     4141 gccagtttcc gcttggctga tacggtcctc tccatcggat agagctacca tatcggtatt
     4201 gaccatagat tgattgtatc ctacaaagcg actgtaaaca ccggtaaata gctcctcgac
     4261 ttccttttgg ctccttataa gccgatcttt ttctccgcgt gcttcacggg ctctattttt
     4321 ttgctgttcc atttgatttt caaattctgt tttatcaaat tgcaaaccct cttcttgaag
     4381 aatatcttca gctacatctg acggaaatcc ataagtatca tagagcatga aaatatcctt
     4441 tccagaaagc tgttgttttt tatttttttt ggtttgttca attaattgac ttaaaaaggc
     4501 ttctccactt tgcagggttc cctgaaagcg ggattcttct tgaaatacaa tttttgaaat
     4561 aatatctttt cgcatgaata gttctggata agcgttactc attccatcaa tgacagtaaa
     4621 tagtaactca tggagaaaag gctgatggat tcctattttt cctccatatc gataggcgcg
     4681 acggatcaag cgccttaaaa tatagcctct tccctcattt gaaggataga ttccatcggt
     4741 aataagaaaa acaatggcac gaatatgatc ggcaataatc cggaaagggg gttttaaggc
     4801 tggtatcaag taatcatttc ctgatatttt ctccaaacct tgaattatgg ggaaaaagag
     4861 atcggtttca tagtcagtat cagtttcctg aacaaccgag gctaaacgct ccaatcccat
     4921 accggtatca atattttttt taggaagttc ctcaagagtt ccatcggctt ttcgatcaaa
     4981 ttccataaaa accagattcc atacttcaag atatctatca caattacacc ccggggcaca
     5041 ggtggactga ccacaaccct ttacttcccc acgatcataa taaatttctg agcaataccc
     5101 acagggacca atcggtcctg aagcccagaa gttatcttca tcacctaaga aaacaattct
     5161 ttccggtggg acaccaacac aattcttcca aacttcgaaa gcttcttggt ctttacgatg
     5221 aactgaaacc cacattctat caggagaaaa accgatcgtc tgagtgagaa attcccatcc
     5281 ccatagacag gcttcttttt taaaataatc accaaaagaa aaatttccta acatttcaaa
     5341 aaaagtatga tggcgggctg tatgaccaac atttcccagg tcattaaccc gaacacattt
     5401 ttggacactg acaacccgct taaaattagg ctggattttg tttaagaaaa ttggtttaaa
     5461 aggcaccata ccagcaatgg ttaaaagcag agttggatcg tttggtatca acgaagcact
     5521 gggaagtatc ttgtgtcctc ttttttcaaa aaaatccagg aatttttgcc tcaactcaga
     5581 actggtcatt gtacgtcact tctctccttt tcaatcacca tcatgctttt catttgctca
     5641 taattatatc caaaaatcga caacctgaaa ggtccgaagt cttcgattgg ttgatggcat
     5701 ccattgaaat gaaactggct gaacaaagtc tggttgaatt ttaccatttt caatccaata
     5761 ccgggaatcg aaatttcggt aaaggccagg attggcacgt attactaagg taagaggacg
     5821 atcaacccag gttatggtat cgatttcttc gaaatcggtc attctttgag aaataatcga
     5881 cttcagcttt ggattgggaa acggtcgaag attaatcagt gcctgattct tctgtaaagg
     5941 tccgacaatt ttaacattag ctatctcgct tttaaatccc tcttgatgga gggtttgcaa
     6001 taaaacctgg ttggctttta ccaccgcctg tccatgaact tttgctgcca gcggtttata
     6061 gagatgtttt aatacgcaac cttctcggac actattttcg cctattcgtc ggacgggaat
     6121 attcataatc tggcaaatct gaaaaatttc ttgcttggag aagtcaaaaa gaggcgaata
     6181 ggtatttcgt atcagcttaa gaccgcggat tccccaacta tcactctgat tagcaccacc
     6241 gattatcagt gttttttccc caaaattctt ttgaagatta tctaatttgg cttttctggt
     6301 gcagagatta caagcaggtc cacggcttac aacctcttca atgatatttc ttccaggaag
     6361 atacaaatgc gggacatgaa aacaggatat aagaaagtca atattctctc gggcatgata
     6421 gggaaaatac tcttcccagt caacggtaca tgcttgcact ttttctctcc ccaaagcata
     6481 tatcgaaaga aaaagtgcaa ccgtactgtc gactcctcca gaaaaggcca ctacgactct
     6541 ttcaaaacct tctgcaatat cttttatttc atttatgaca ttctggatcc gcttctcaaa
     6601 aacaacatca acaacttcta tcatgaatta tttttcatat tattgagaag attattaaaa
     6661 gtatctccaa aaacatcgcc caaagtagtt ctaccattgg tttgagttat tggttgttct
     6721 tttttgggtt tttccctttc ttttgttcgt tctttttctt catcacgagg gagagtttgc
     6781 ttaatgctca gaccaatttt tctctcctgg tcatccagtt taataatctt tacattgact
     6841 tcttgattct cttcgattac ttctgaggga gaggaaattc gtttatcaga tatctctgaa
     6901 atgtgaacta aaccatccca tcccttttcc aattcaataa aagctccaaa atccacaatt
     6961 ctgacaactt tgccggtatg aatacttcct actgggtatt tattttttat ttcgaaccaa
     7021 gg
//
LOCUS       DAOSOL010000036         7000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10811_length_7000_cov_4.012527, whole genome shotgun sequence.
ACCESSION   DAOSOL010000036 DAOSOL010000000
VERSION     DAOSOL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10811_length_7000_cov_4.012527"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..2096
                     /gene="dnaK"
                     /locus_tag="PK171_01645"
     CDS             171..2096
                     /gene="dnaK"
                     /locus_tag="PK171_01645"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOT04940.1"
                     /translation="MAKVIGIDLGTTNSVVAYMEGGQPTIIPNKEGSRLTPSIVAFTK
                     NGEILVGQLAKRQAITNPKNTIYSIKRLMGRRYDDPEVEKARKVLPYEIVPGKHGEAA
                     VKIGDRVYTPPEISAMILKKMKEDAEEFLGEKITEAVITVPAYFNDNQRQATKDAGKI
                     AGLEVKRIINEPTAASLAYGLGKGKEEVIAVYDLGGGTFDISILEIGEGVFEVKSTSG
                     DTFLGGDDFDRRVMDYIIEEFRKDQGIDLRGDQMALQRLKEAAEKAKIELSGSLQTEV
                     NLPFITADASGPRHLVMTITRAKLEQLTADLIERTFEPCERAMKDAGLKPENIDTVIL
                     VGGMTRMPKVEEVVKRIFQKEPKRGVNPDEVVSDGAAIQAGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTRIIERNTTVPVSKSQVFTTAADNQTTVEIQVLQGERPLAKDNNSLG
                     KFQLSGIPPAPRGVPQVEVKFDIDADGILHVSAKDLATNKEQAITIKASSGLSKEDID
                     RMLRESELHAEEDKKKQEEIELKNQAENLTYSVEKTLRESGGKLSAETKSKAEAALND
                     LKEAVKGDDVADIRRKMDELQSTSHALAQELYRQTSGQQGPGEAAGAKAETSSDTNKE
                     NDNVVDADYKVVDDDQK"
     gene            2277..3431
                     /gene="dnaJ"
                     /locus_tag="PK171_01650"
     CDS             2277..3431
                     /gene="dnaJ"
                     /locus_tag="PK171_01650"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOT04941.1"
                     /translation="MKKDYYEVLGVNRNASLEEIKKSYRRLARQYHPDANPGNKDTVE
                     KFKEINEAYQVLSDTEKRTTYDRFGHAAFDSRAQAGYGGGGFGQDFDPFGDFGSFGDI
                     FDMFFGSSTGSRRRDSASRPTKGANINVDVTLEFEEAAFGVEKEIEYSRIEFCKECQG
                     VGGKKKVSCPHCRGTGEIKHTQTSIFGSIVTSRICNFCHGKGYVAEDTCQKCHGSGKV
                     KTTRKLKLKFPAGVDSGYRLRVSGEGEAGENDGPAGDLYVSITVKPHKILERKGKNLF
                     FDAEVTYTQLVLGDEIEVPTIQGMEKIKISPGTDTHSVLKLRGKGLVDPSNGSRGDQF
                     VQLKLRIPRRLTGEYKKLMEQLAELEGQEKNKKSGGIFERIKEAFTSTEG"
     gene            3442..4182
                     /locus_tag="PK171_01655"
     CDS             3442..4182
                     /locus_tag="PK171_01655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT04942.1"
                     /translation="MSRFYYHPEINQSTILALSHEESLHLKTNRIQPETEILLSDGLG
                     SLYSGIFKGFVSQLAQVEVVGKVKKEDNAYQLWIWQPLLKNWSRLEWLIEKLVEVGVS
                     GIGIFYCERCVRERISSTKEERWRKIIIEAAKQSGRIRFPILKVITNWSDFLTHCRSN
                     NLNTIIGDFSSSESISDYLKEQSSFSLQIIVGPEGDFSEGEKEQLHSLPKVCFLRFSN
                     QILRSETAALYASIVSIASLESKHENCY"
     gene            4166..5446
                     /gene="mtaB"
                     /locus_tag="PK171_01660"
     CDS             4166..5446
                     /gene="mtaB"
                     /locus_tag="PK171_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOT04943.1"
                     /translation="MKIAIKTLGCRVNQSESDILAALLKSEDISLVPFEDQADWYIIN
                     SCAVTKMSEKKTRQWISRALRNNETSQVILIGCYSPLYTRQFPLPQPRLQVIDSPEKI
                     KKVMEKLNLPWNDSFSEKVIPETKRARVWLKIEDGCDHFCNYCIVPHLRGAVRSEEPE
                     KIIHQAIHLERKGVHEIVLCGINLGRYDFGSNHTTLIELLEELIRATNIVRYRLSSIE
                     PFLIDKKFLDRYFSLIPRVCPHFHIPLQSGSDEVLLKMGRGYTTQFYRELVELIRSYD
                     RNVAISTDIIVGFPGESEKLFTETIEFCQEIGFSRAHVFVYSPRPFTPASQWDKIEGV
                     PFQAKKDREAQLLKIIYNSQIKYYRSFIGKTLSVLVEATTENHVITGYSDNYIPVRGE
                     GILASDYGRILPIFIQGEQDRILLGKAIQNQYTV"
     gene            5510..>7000
                     /locus_tag="PK171_01665"
     CDS             5510..>7000
                     /locus_tag="PK171_01665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT04944.1"
                     /translation="MAAKKKIKTVKITKSKLPVVESPERTLPLLEKFLQYGLLLIVLL
                     SPYYRGLYFDYERYPFFLAVFLLAIIYFFYQIAYQRKVITIKTPVEYFFLLTVLLYGL
                     SIFWAADQGMAFREFFSSVAYFIFFLLVTNLFLTKHSKKILLITFNINAVLLVLLGFF
                     YRFGWINPLSRPLGMSMKDLFLAASGRLHSTMQYPNTFAAYLAMAIITLILIHFLEEK
                     PVYLSFWGFILFFLQTGLYFTYSRGALLVFIITSVVLFLLLKRQEKIKHLLILMVAFI
                     WTILFTPSLENFLFNNLPGQFFGFLLLGSLLQAFSIYLLSFLWKKLINLSKPSIRTSY
                     LTVGIGVALVLVILIILGLKPAFLGDRFRQITLNTIISQERWIFYGDGLKIFQTRPTV
                     GWGGGGWEACYFAHQSYPYFSENTHNYFLQVMIEIGIIGLLATIGLIIGLFYQIYRFK
                     KVNSDNWETLFVLGLGVAVFLGFFHGFIDVDFALGSFYFGVWVFIAF"
BASE COUNT         2199 a         1160 c         1488 g         2153 t
ORIGIN      
        1 aaatatcatg aggcaatgat gacaatatca tcccctgatt atgaggatta tgcaattgtc
       61 gatgagttaa gaaaaggata tatgttgaat gaagaagttt tacgtgcagc ccaggtatcg
      121 gttaatcgtt gggataaaaa tgagaaagaa aggtaaagga ggaaaacact atggcaaaag
      181 taatcggtat tgatttggga acaaccaatt ctgttgtggc ttatatggag ggtggtcaac
      241 caaccattat acccaataaa gaaggttctc gtttaacacc ctcaattgtt gcatttacta
      301 aaaatggtga aatattggtt ggtcagttag ccaaaagaca agcaataacc aatccaaaga
      361 ataccattta ttctataaag cgtttaatgg gtcgacgtta tgatgatcca gaggttgaaa
      421 aggcgcgtaa agtacttcct tatgaaattg tacccggaaa acatggagaa gctgctgtaa
      481 aaattggaga tcgagtatac actcctcccg aaatatcagc aatgatatta aagaaaatga
      541 aagaggatgc cgaggaattc ttaggagaaa aaataaccga agcagttatc actgtcccgg
      601 cgtactttaa tgataatcag cgtcaggcaa ccaaggatgc tgggaaaatt gccggacttg
      661 aagtaaaaag aatcataaac gaaccaaccg cagcttcatt ggcttatggt ttgggaaagg
      721 gaaaagagga agtcatagcc gtgtatgatt taggtggcgg gacttttgat atttcaattc
      781 ttgaaattgg tgaaggtgtg tttgaggtaa aatccacctc gggagataca ttccttggtg
      841 gtgacgattt cgaccgaaga gtaatggatt acattattga agagtttcgt aaagatcaag
      901 gcatcgatct tcggggagat caaatggctc tgcagcgttt aaaagaagcc gctgaaaaag
      961 ccaaaattga attatccggt tcgctccaaa ccgaagtgaa tttacccttt attactgccg
     1021 atgcatctgg gcctcgacat ttagtgatga ctatcactag agcaaaatta gagcagttaa
     1081 ccgcagatct tattgaacgt acctttgaac cctgtgaacg ggcaatgaaa gatgctggcc
     1141 ttaagccaga aaacattgat acagtcattt tggttggtgg tatgacccga atgccaaagg
     1201 tagaagaagt cgtcaaaagg atattccaga aagaaccaaa acgtggagtc aatcccgacg
     1261 aagtagtttc tgatggagca gctattcaag ctggagtatt aaaaggagaa gtaaaagacg
     1321 ttcttctgct agacgtaact ccactttctt tgggaattga aactttggga ggagttttta
     1381 ctcgtatcat tgagcggaat acgacagtgc cggtttcgaa gagtcaagtt tttaccacag
     1441 ctgctgataa tcaaacaaca gttgagattc aggtgctcca gggagagcgg cctttagcaa
     1501 aagataataa ttctcttgga aaatttcagc tgtctggaat tccaccggca ccacggggag
     1561 ttcctcaggt tgaagtcaag tttgatattg atgctgatgg aattctccat gtatcggcca
     1621 aagacttggc gactaataaa gaacaagcca taacaataaa agcttccagt ggtttatcga
     1681 aagaagacat tgatcgtatg ttgcgagagt cagaacttca tgccgaagag gacaagaaaa
     1741 agcaagaaga aattgagttg aaaaaccaag cagaaaacct gacctattca gtagaaaaaa
     1801 ccttgagaga gtcaggaggc aagttgtcag ctgaaacgaa gagtaaagca gaagctgctt
     1861 tgaatgacct caaggaagct gtgaaaggtg atgacgttgc tgatattcga cgtaaaatgg
     1921 atgaactcca atcaacttct catgctttag ctcaggagct ataccgacaa acctctggcc
     1981 agcagggtcc tggtgaggca gcgggagcaa aagctgaaac aagttcggat acgaataaag
     2041 aaaatgataa tgtggttgat gcagattata aagtcgttga tgatgatcag aagtaagtga
     2101 tggaaaatcc ttgggtcaaa taatagtttg gcctaaggat tttcctttta ttaaatctcc
     2161 ccattgatct tcaatactat taattatttg aacttctttg atttttttac aaaattgata
     2221 taaagtaatc gtattcttct ccgaataata tggaaataat taaaggtgat ttgtgcatga
     2281 aaaaggatta ttacgaagtt ttgggagtga atcgtaacgc ttccttagaa gagataaaga
     2341 aatcctatcg aaggctggca agacaatacc atcctgatgc caatcctgga aataaagata
     2401 cggttgaaaa atttaaagaa atcaatgaag cctaccaagt tctttctgat acggaaaagc
     2461 ggactactta tgatcgtttt ggccatgccg cttttgactc ccgtgctcaa gccggttatg
     2521 gtggaggagg ttttgggcaa gactttgatc cgtttggaga ttttggaagt tttggtgaca
     2581 tctttgatat gttttttgga agttctaccg gatcacgacg aagagactca gcatcccgtc
     2641 caacgaaagg ggcaaatatc aatgttgatg ttacgcttga atttgaagaa gccgcttttg
     2701 gagtggagaa agaaattgaa tattcaagaa tagaattctg taaggaatgc cagggagttg
     2761 gtgggaaaaa gaaagtctcc tgtcctcatt gtcgaggtac cggagaaatt aaacatactc
     2821 aaacttcaat ttttggttca atagtgactt cacgtatttg taatttttgt cacggaaaag
     2881 gttatgtcgc tgaagatact tgtcagaaat gtcatggttc agggaaggtg aaaacgaccc
     2941 ggaaactaaa attaaaattt ccagctggag ttgatagtgg ctatcgactt cgagtatccg
     3001 gggaaggaga agccggggaa aacgatggtc cagctgggga tttgtatgtt tcaataacag
     3061 taaaaccaca taaaatctta gagaggaaag gcaaaaatct cttctttgat gccgaggtga
     3121 cttataccca gctagtttta ggtgatgaaa tcgaagttcc taccatacaa ggcatggaga
     3181 aaattaaaat ttcacccggg acggacactc attcagtttt aaagttaaga ggtaagggtt
     3241 tagttgatcc aagtaacggt tcacggggtg accagtttgt tcaactcaag cttcgcatac
     3301 caagacgatt aaccggtgaa tataaaaaac tgatggagca attggccgaa ttagaaggtc
     3361 aggaaaaaaa taaaaaatca ggtggaattt ttgaacgaat aaaagaagct ttcaccagca
     3421 ctgaaggtta gaaaaattag aatgagtcga ttttattacc atccagaaat aaaccaatca
     3481 actattcttg ctcttagcca tgaagaaagt cttcacctca aaaccaatcg aattcagcct
     3541 gaaaccgaga ttctgttatc agatggatta ggtagtttat attcaggcat atttaaagga
     3601 tttgtttcgc aattagccca agtcgaagtt gttggaaagg taaaaaaaga agacaatgct
     3661 tatcagctgt ggatatggca acctcttcta aaaaattggt cccgtttaga atggctcata
     3721 gaaaaattgg tcgaagttgg tgtttctggt attggaattt tttattgtga gcgatgtgtt
     3781 cgtgaacgta tttcttcaac gaaagaagaa cgttggagaa agattattat cgaagcagcg
     3841 aagcaatcag gaagaatacg ttttcctatt cttaaagtga ttacaaattg gtctgatttt
     3901 ctcactcact gccgaagcaa taacctgaat actattattg gtgattttag ttcctcggaa
     3961 tcaatatcgg attatttaaa agaacaaagt tcattttctt tgcaaattat tgttggcccg
     4021 gaaggtgatt ttagtgaagg tgaaaaagaa cagcttcatt ctcttcccaa agtttgtttt
     4081 ttaagatttt ccaaccaaat tctacgaagt gaaactgctg ctttatatgc cagtattgtg
     4141 agtatagcca gcctggaatc gaaacatgaa aattgctatt aaaactctcg gatgtagagt
     4201 aaatcagtca gaaagtgata ttcttgctgc actattaaaa agtgaagata tttctttggt
     4261 tcctttcgag gatcaagctg attggtacat tattaatagc tgcgcagtga ccaagatgtc
     4321 ggaaaaaaaa actcgtcaat ggatcagccg agctttaagg aataatgaaa catcacaagt
     4381 tattcttatt gggtgttata gccctttgta tacccgtcaa tttccacttc ctcagcctcg
     4441 tctccaggtt attgattctc cagagaagat caaaaaggta atggaaaaac tcaatttacc
     4501 ttggaatgat tctttctcag aaaaagtcat accagaaacc aaacgggctc gggtctggtt
     4561 aaaaatcgag gatggatgtg atcatttctg caattattgt attgtacccc acctccgtgg
     4621 tgctgttcgg agtgaagaac cagaaaaaat aattcaccaa gcaattcatt tagaaagaaa
     4681 aggtgttcat gaaattgttt tgtgtggtat taacttagga aggtatgatt ttgggagtaa
     4741 tcatactacc ctgattgaat tactggaaga attgataaga gcaaccaata tcgttcgcta
     4801 tcgtttaagt tctattgagc cgtttctgat tgataaaaag tttttggatc gatatttttc
     4861 tttgatccct cgagtatgcc cgcattttca tatccctctc caaagtggga gtgatgaagt
     4921 tttattaaag atgggaaggg gttataccac tcaattttat agagaactag tagaattaat
     4981 tcggagttat gatagaaatg tagcaattag tactgacatt attgtaggat tccctgggga
     5041 atcggaaaaa ctgtttactg aaacaattga attttgtcag gaaatcggtt tttcccgtgc
     5101 tcatgttttt gtttattctc cacgtccttt tactcctgct tcccagtggg ataagattga
     5161 aggggttcct tttcaagcca aaaaagaccg agaagcacag ttattaaaaa ttatttacaa
     5221 cagccaaatc aaatattatc ggtcttttat tggaaaaaca ttatcagtat tggttgaggc
     5281 aaccactgaa aaccatgtga taactggtta ttctgataat tacataccag ttcgagggga
     5341 gggaattttg gcttctgatt atggaaggat tcttcctata tttatccagg gagaacagga
     5401 tagaatttta ttagggaaag ctattcaaaa ccaatatact gtataatccc attgaaaatt
     5461 cattttatat tcaataaaat agagagaaaa ctggagaagg aaagataaaa tggcagctaa
     5521 gaaaaagata aagacggtaa aaataaccaa gtcaaagctt cctgtagtcg aatctcccga
     5581 aagaactctt ccacttttag aaaaatttct tcaatatggt ttattgctga ttgtgctttt
     5641 atccccttat taccggggtt tatattttga ttacgaaaga tatccctttt ttttggcggt
     5701 tttcctcctt gccattattt attttttcta tcaaatagct taccaaagaa aagtaattac
     5761 tataaaaact cccgtcgaat attttttcct ccttaccgtt ttattatatg gtctcagtat
     5821 tttttgggct gcggaccaag ggatggcttt tcgtgaattt tttagtagtg tagcttactt
     5881 tatctttttt cttttggtca caaatttatt cctgaccaag cattcaaaaa aaattctttt
     5941 aataacattc aatatcaatg ctgttttact ggttttgctg gggtttttct accgatttgg
     6001 gtggattaat cctctgtctc gaccattggg gatgagcatg aaagatcttt ttttggcagc
     6061 atcaggtcgt ttgcattcaa ctatgcaata cccaaatacc tttgctgctt atctcgcaat
     6121 ggcgataata accttgattc ttattcattt tttggaagaa aaaccggttt atctcagttt
     6181 ttgggggttt atactttttt ttcttcaaac tggattatat tttacctatt cccggggagc
     6241 tttgttggta tttatcataa cctcggtggt tttattcctt ttattgaaaa ggcaagagaa
     6301 gataaagcat cttcttattc tcatggtcgc atttatttgg acaatacttt ttactccgag
     6361 cttggaaaat tttttattca acaatttacc cggtcagttt tttggtttcc tcttattagg
     6421 gagtctttta caggctttta gcatctattt gctttccttc ctatggaaaa agttgatcaa
     6481 cctatcaaaa ccatcaattc gtacctccta tttaacagtt ggaattggtg ttgctcttgt
     6541 tctcgttatt ttaattattt tgggactaaa accagccttc ttgggtgatc gttttcggca
     6601 aatcactctc aacaccatca tttctcaaga aagatggatt ttttatggag atggtttaaa
     6661 aatattccaa acccgaccaa ccgttggttg gggaggagga ggatgggaag cttgttattt
     6721 tgcccatcaa tcctatccct attttagcga aaacactcac aattattttc ttcaagtgat
     6781 gattgaaatc ggcattattg gtttgttagc aacaattggg ttaataattg gcctttttta
     6841 tcaaatttat cgtttcaaaa aggtgaatag tgataattgg gaaactcttt tcgttttagg
     6901 attgggagtt gcagtgtttt taggattttt tcatggattc attgatgttg attttgccct
     6961 tggttctttt tattttggcg tttgggtgtt tatcgctttc
//
LOCUS       DAOSOL010000037         6960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10914_length_6960_cov_3.946850, whole genome shotgun sequence.
ACCESSION   DAOSOL010000037 DAOSOL010000000
VERSION     DAOSOL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10914_length_6960_cov_3.946850"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(234..1046)
                     /locus_tag="PK171_01670"
     CDS             complement(234..1046)
                     /locus_tag="PK171_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOT04945.1"
                     /translation="MHFIAFLTDWGMTSYYVGIAKSVMKQINPGVEIIDITHDIEPFN
                     IREAMYILQRTFPDFPPGTIFCSVVDYGVGTERFPIAVELANGSFLVGPDNGTFTLLI
                     EQYPTKKTVILQNPSYFFRLNPSSTFHGRDIFAPVSARLSMGVPLEHFGPVVNDLHTL
                     TVNQPNLHNHTITGEVAFCDRFGNIETNIPGIMLNNFELQPGDPLNIVINGRSFDAVF
                     FEAYGLSKKGQILVHTDSSGFVEIAVNQGNAREALLDNKDISQISILNKKIK"
     gene            complement(1269..2459)
                     /locus_tag="PK171_01675"
     CDS             complement(1269..2459)
                     /locus_tag="PK171_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT04946.1"
                     /translation="MDKKNKRKNILITGLTSLLTDISSEMIYPIIALYLKALGGSPAI
                     LGIIEGVAESTASILKVFSGAFADRIGKRKPLAIAGYSFSVIGKVLFYFASNWSFIFA
                     GRFSDRFGKGVRTAPRDAMISESVEGNHQGKAFGLHRTMDSMGAVIGTLITFFFLFRI
                     QEAARQSGNPAFYIPTFRSIILLSLIPAALGVVVLFLTIETGSGEKSTHIPLSWSSLL
                     KLDHKLKVFLLATFLFTLGNSSDQFILLRATEPDLGYSAAQVILLYLLFNIVYMVVSY
                     PAGWLSDKIGQKWILAGGFLLYSITYYVIGFTPSFIIPAMILYGFYIGMTEGVAKALV
                     SELSPASRRATIIGLHATLLGIGLFPASLIGGLLWSTFGPSATFLFGGSMTFISALIL
                     ILFL"
     gene            2646..3197
                     /locus_tag="PK171_01680"
     CDS             2646..3197
                     /locus_tag="PK171_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOT04947.1"
                     /translation="MDNNRLIIKSSKINRLIYIAILVAMGSVLHGLEGLIPLPNLFIP
                     GAKLGFANIVTLIAMVLLGQNEALLVTLLRVFLGGLISGNFLNIGFFLALSGGIFAWL
                     GMKLMSKATESVLIISMFGALFHNIGQILVAYLYIQSIHIFWYLLLLFPFAILAGFFT
                     GYLSSLIIHYVRNSSVSLNRNYK"
     gene            3477..3992
                     /gene="greA"
                     /locus_tag="PK171_01685"
     CDS             3477..3992
                     /gene="greA"
                     /locus_tag="PK171_01685"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOT04948.1"
                     /translation="MEKKKSDEKIILTKEGYETLEKELKHLKTVKRKEVAEKINQALA
                     FGDLSENAEYEEAKNEQAFIEGRILVLEEKLMKATIVEEQSGGNSEKIHLGVRVMLEN
                     LNNGKQIEYSIVDSVGANPSLQKISFESPLAQALIGKTKGDEIELKVPAGMVKYRVID
                     IKKKEVNSISS"
     gene            3989..5473
                     /gene="lysS"
                     /locus_tag="PK171_01690"
     CDS             3989..5473
                     /gene="lysS"
                     /locus_tag="PK171_01690"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOT04949.1"
                     /translation="MNEEAREQLDQINVEEQTKKLQELRNLGIDPYGVPFPEKHSIQD
                     IRSHPEKFLDQDVLLRIAGRIMAIRRHGKAIFADVQDRLGRIQIYVKKDTIGDDSFGI
                     FKSLDVGDVIGLEGKLFKTHSGELTIVVENFSLLSKCLHPLPEKWHGLREVEVRYRKR
                     YLDLIMNPEVRKVFQTRSRVLSELRKYLDERDYLEVETPMMQLIPGGALARPFVTFHN
                     ALGIDLYLRIAPELYLKRLIVGGMEKVYEINRNFRNEGISVRHNPEFTMLELYQAYGN
                     YETMMNLCEKMLSSIVFSILGTYEVNYHDMAIDFTPPWRRLNLREVIQKEGGIDIFED
                     SLETLYQRGKKAGLNCDQTWDRGKYVNEFLDIFVQPHLVNPTFVLEYPVEISPLAKAK
                     KNDPLVAERFELFIGKEELGNAYSELNDPIEQKKRFLDQVKKRDRGDVEAHLIDQDYV
                     EALEYGMPPTGGMGIGIDRLVMLLTNSSSIREVIFFPILRPKID"
     gene            5470..6096
                     /locus_tag="PK171_01695"
     CDS             5470..6096
                     /locus_tag="PK171_01695"
                     /inference="COORDINATES: protein motif:HMM:NF017648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOT04950.1"
                     /translation="MTFEKFIPIIGNVNNSQNTRWPFLTAEFELEVTAKKTENGRSVE
                     MYSLCDSIEKYLIRLIEKSPNNTVTIQRGRVAEEFDCAPSQINYVLMTRFNVFKGFIV
                     ESRRGGSGYVKIQQIQMDRLEPVVNFLEVSEKDIKESEVESLLLWLEREGFITSREGS
                     LMKAAIIDTFNRLNFDSLLSVRDVNILRSNILREMLLQVLKVGYDYEV"
     gene            6086..6586
                     /locus_tag="PK171_01700"
     CDS             6086..6586
                     /locus_tag="PK171_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04951.1"
                     /translation="MKCELCNDNIASVVFFVLDKEEKIEKKLYLCEFCANKVPVYSFV
                     VNQNQQSLHLKKFFLPKETDENTSVLSCSYCLTSAKEFLESGLLGCVHCYDTFQELIR
                     DLITEKQPEIIHRGKVPVRLFRRKKIKKDIDHLRQIYQRCLEEENYEQASGVVRRLKQ
                     LESYLR"
     gene            6628..>6960
                     /locus_tag="PK171_01705"
     CDS             6628..>6960
                     /locus_tag="PK171_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04952.1"
                     /translation="MSKVISRISLSRNIEDIPFPSRATSIWIEDLKQRIKNLYQKIFA
                     AKKYQWVQLDTIPKHALEKFYYQNLIPREVLENPANRYLIYSSHKGILVNYLDHLQIF
                     SITSSLDLS"
BASE COUNT         2298 a         1151 c         1442 g         2069 t
ORIGIN      
        1 aaattttatt aattactttt catttactct ggtattttca ggatagaccc tcatgctgct
       61 ttcgcagcac cagatgagga tgaaacctaa ggttcgacat gccatggcag gtcgctacat
      121 taattaaaga atgggcacaa tacattgtgc ccctacaatt ttatattctt tggtaggggc
      181 ttgatttatc aagcccgtgg tttttcaggt tagattttta tatatggata ttgttatttt
      241 atttttttgt tgaggattga tatttggcta atatctttat tatcaagtag agcttccctg
      301 gcatttcctt ggttcacggc tatttcaaca aaacccgagc tatcggtatg cactaaaatc
      361 tgtccttttt tagacaatcc atatgcctca aaaaaaaccg catcaaaaga acgtccatta
      421 atgacaatgt tcaaaggatc tccgggttgt agttcaaaat tattcagcat tattccggga
      481 atattcgttt ctatgttgcc aaaacggtca caaaaggcga cttcgccggt tatagtgtgg
      541 ttatgaaggt tgggttgatt gactgtaagc gtatggaggt cattaacgac cggtccaaaa
      601 tgttccaagg ggacacccat tgagaggcga gcgctcacag gagcaaaaat atcccgacca
      661 tgaaaggtgc tactgggatt taagcgaaaa aagtaagaag ggttctggag aatgactgtt
      721 tttttcgtgg ggtactgttc gatgaggagt gtaaaagtcc cattatcagg accaaccaaa
      781 aatgaaccat tggcaagctc gacagcaatt gggaagcgtt ccgttcctac tccataatcg
      841 actactgaac aaaaaattgt tccgggagga aaatcaggga aagtacgttg aagaatatac
      901 atggcttcac ggatattgaa gggttcaata tcgtgagtga tgtcgatgat ttcaacaccc
      961 gggttaatct gtttcataac tgatttagca attcctacgt aataactggt cattccccaa
     1021 tcggttaaaa aggcgatgaa atgcatcttt agcctccata attccaatca ttcttataag
     1081 agttggtatg attattatat attatctgat aaatttagtg agatatgggt taagagtaga
     1141 ataataattt ggaatttgaa gctttgaaag tgtgaaaaac catgaaaata cttctttctt
     1201 ttatcaaggg agaaggagca aatagatcag agggagaagg gacctaactg caaaatagct
     1261 gctgatgttt atagaaataa aataagaatt aaagccgaga taaatgtcat tgatcctcca
     1321 aaaagaaaag ttgccgaagg gccaaaagta ctccagagta atcccccgat taaagaagcc
     1381 gggaagagac caattcccag taaggttgca tgaagaccga taatagtagc tctccttgaa
     1441 gcaggtgaaa gctcagaaac cagtgctttg gcaacccctt cagtcatacc gatatagaaa
     1501 ccatagagaa tcatggccgg gataataaag gaaggagtaa aaccaatcac ataataagta
     1561 atactataaa ggagaaaccc tccagctaat atccactttt gaccaatttt gtcggaaagc
     1621 catcctgccg gataagaaac caccatatat acaatattaa agagcagata gagaagtatc
     1681 acttgagcag cgctataccc taaatcaggt tcagttgctc ttaataagat gaattggtcg
     1741 gaggaatttc ccaaagtaaa caaaaaggtg gccaacagga aaacttttaa cttatgatcc
     1801 agtttaagaa gtgaactcca agaaagtgga atatgagtac ttttttcccc acttccggtt
     1861 tctatggtca agaaaagaac aaccacccct aaagctgccg gtatcaagct gagaagaata
     1921 atggagcgga aggtgggaat ataaaaagca ggatttcccg attgccgggc agcttcttga
     1981 atacgaaaga gaaagaaaaa ggtgattaag gtaccgatta ctgcacccat ggaatccatg
     2041 gtgcgatgga gtccaaaagc ttttccttga tgattccctt caactgattc agaaatcatg
     2101 gcatcacggg gagcagtacg aactcctttc ccaaaacgat ctgaaaatct tccagcaaat
     2161 ataaaactcc aattactggc aaaataaaat agtacttttc ctataacaga aaaggaatac
     2221 ccagcaattg ctaaaggctt tcttttgcct attcggtcag caaaagcccc agaaaacact
     2281 ttgagaatcg atgccgttga ctcggcaaca ccttcgatga tgccgagaat agccggactg
     2341 cctcccaagg cttttaaata aagagcaatg atcgggtaga tcatttctga actgatatcg
     2401 gtcaagagtg aagttaaacc ggtaataaga atgttttttc ttttattttt tttatccatt
     2461 tcagtctcac cattggttta taatatcttt agattaaagg cctatattta atattgttga
     2521 agcaaatgaa ataataatta tattcagtat gtctattctt attataaacc aaatatgaca
     2581 gctgtcattc ccaaagcaat cgggaacttt ttaaaatcat agttttcgaa gatttgagat
     2641 gaaacatgga caataaccgt ttaattatta aatcaagtaa aattaatcga ttaatatata
     2701 ttgcgatttt agtagctatg ggaagtgttc tgcatggatt ggaaggcttg atcccgctgc
     2761 ctaacttatt tattcctgga gcgaagttag gttttgctaa tattgttacc ttgattgcta
     2821 tggtattatt aggtcaaaac gaggccttgc tggtcacctt gttgagagtt tttttagggg
     2881 gattaatttc tggaaacttt ctcaatattg ggttttttct agctttaagt ggtggaatat
     2941 ttgcttggtt aggaatgaag ttaatgagca aagccaccga atcagttctc atcatcagta
     3001 tgttcggagc ccttttccat aatatcggcc aaatattagt cgcttatcta tatattcaaa
     3061 gcatccatat tttttggtat ttgctattat tgtttccctt tgctatttta gccggttttt
     3121 tcaccggata tctttcgtct ctcattattc attatgtaag aaattcctcg gtttcactga
     3181 acagaaatta taaataggag gaaaaattac cgacctaaga agtgataatt caaaatattc
     3241 attctttcaa tgttgtcagt ggaaaaacaa aatatgacaa aaggaaaaaa atgacttgaa
     3301 tcatagttaa gaaaaaagta taattcatat ttcaataata aagatatcct tccgcgagaa
     3361 aacagcagca atgaaccttc tagagcaaga ttatctcctt ggggataact tgacaacccg
     3421 gtaacctttt tatatggtat ttcgatagcg gaaaaccttt tcaggaggtt taattgatgg
     3481 agaagaaaaa gtcagatgaa aagattattc ttaccaaaga gggatatgag accttagaaa
     3541 aagagttaaa gcatctcaaa acagtgaagc ggaaagaggt agcagaaaag atcaaccaag
     3601 ctttagcatt cggtgattta agtgaaaatg ctgagtatga agaagctaag aatgagcaag
     3661 ctttcattga aggacgtatt ttggttttgg aagaaaagct gatgaaagcg acaatcgttg
     3721 aagaacaaag tggaggaaac tcagagaaaa ttcatttagg ggttcgagtg atgctcgaaa
     3781 atctcaacaa tggaaaacag attgagtatt cgatagtcga ctcagttgga gccaatcctt
     3841 ctctccaaaa gatttctttt gagtcacctc tggctcaagc tcttattggg aaaaccaagg
     3901 gagatgaaat tgaattaaaa gtacctgctg gaatggtaaa atatcgagtg atagatataa
     3961 agaaaaagga ggttaattcc atttcatcat gaatgaagaa gcgcgggagc agttggacca
     4021 aatcaatgtt gaagaacaaa ccaaaaaact acaggaatta agaaatttag gtattgatcc
     4081 ttatggagtt ccctttcccg agaaacattc cattcaagac attcgatctc atccggaaaa
     4141 atttctggat caagatgttc tcttacggat tgccggtaga ataatggcta ttcgtcgcca
     4201 tggaaaagcc attttcgctg atgttcagga tcgactggga agaattcaaa tctacgttaa
     4261 gaaagatacc attggtgatg attcttttgg gatttttaaa agtctagatg taggggacgt
     4321 tattggttta gaagggaaat tgttcaaaac ccatagtggt gagctgacta ttgtggttga
     4381 aaattttagt cttctcagta aatgcttgca tccacttcca gagaaatggc atggattaag
     4441 agaagttgaa gtgagatatc gaaaacgtta tcttgattta attatgaatc ctgaagtcag
     4501 aaaagtgttt caaactcgtt ctcgagtgtt gagtgagctc agaaaatatt tagatgaaag
     4561 agattattta gaagttgaaa ctccgatgat gcaactcatt ccaggaggag cgttagctcg
     4621 tccctttgta acttttcaca atgcgcttgg aattgactta taccttcgga ttgctccaga
     4681 actttatctc aaacgcctta ttgttggcgg tatggaaaag gtatacgaaa taaaccgaaa
     4741 ttttcgcaac gaggggatat cagttcgcca taatccggaa tttaccatgt tagagcttta
     4801 ccaggcttat ggtaactatg agacgatgat gaatttatgc gaaaaaatgt tgtcatcgat
     4861 tgtattttcc atcttgggga cttatgaggt aaattatcat gatatggcta ttgattttac
     4921 tcctccgtgg cgaagactaa accttcgaga agtaattcaa aaagaaggtg gaatagatat
     4981 ctttgaagat agccttgaaa cactgtacca gcgagggaaa aaagcaggtt taaactgcga
     5041 tcagacctgg gatcggggaa aatacgttaa cgaatttctg gatatttttg ttcaaccaca
     5101 cctggtgaat ccgacttttg ttttagaata tccggttgaa atatcaccgt tggccaaagc
     5161 caaaaaaaat gatcctttag ttgcggaacg atttgagctt tttattggta aggaagaatt
     5221 aggaaatgcc tatagcgagc tcaatgatcc tatcgagcaa aagaagagat ttttagatca
     5281 agttaaaaaa agagacaggg gtgacgttga agcccatctt atcgatcaag attatgtaga
     5341 agcacttgaa tatgggatgc cgcctaccgg tggaatggga attggtattg atcgactggt
     5401 tatgctttta accaattcat cctcgattcg agaagttatt ttcttcccta ttctccgacc
     5461 gaaaattgat tgacattcga gaagtttatt cctataatag gaaatgtcaa taatagtcaa
     5521 aatactcgat ggccgttttt gacggcagaa ttcgagctgg aagtgactgc caaaaagacc
     5581 gagaatggga ggtcggtaga gatgtactcc ctttgcgact ctattgaaaa atatttaata
     5641 cgacttattg aaaaatcacc caacaacact gtaactatcc aaagaggtag ggtggctgag
     5701 gagtttgatt gcgcaccttc tcagatcaat tatgtattaa tgacccgctt taatgttttt
     5761 aaaggcttta tcgtcgaaag ccgaagggga ggaagcgggt atgtcaaaat acaacagata
     5821 caaatggatc gcttggaacc ggtagttaat tttctcgaag tatccgaaaa agatatcaaa
     5881 gaaagtgaag tagaaagttt gttgttgtgg ttggagcgag aaggattcat tacctcaaga
     5941 gagggttcgt taatgaaagc agcgataatt gatactttta atagacttaa ctttgattca
     6001 cttctttcgg ttcgggatgt aaatatattg cgatccaata tccttcggga aatgcttctt
     6061 caagttttaa aagttgggta tgattatgaa gtgtgaacta tgtaatgata atattgccag
     6121 tgtagttttt tttgtcttgg ataaggaaga aaagattgag aaaaagctat atttgtgcga
     6181 attttgcgct aataaggtac cagtttatag ctttgttgta aatcagaatc aacaatcatt
     6241 gcatttaaag aaatttttcc tgccaaaaga aactgatgag aatacgtctg ttttatcttg
     6301 tagctattgt ttaacttctg caaaagaatt tttagaaagt ggtttattag gatgtgttca
     6361 ttgttatgat acttttcagg agctgatacg agatttaatt actgaaaaac aaccggagat
     6421 catacatcgt ggaaaagtac ctgttcggtt gtttcgaaga aaaaaaatca aaaaagatat
     6481 cgaccatctg aggcagatat atcaaagatg ccttgaggaa gaaaattatg aacaagccag
     6541 tggtgttgtc cgaagactca aacagcttga gtcatatcta agatgagaat atttttttca
     6601 aatttttttt ctcaaaaacc agataatttg tcaaaggtta tttctcggat atctttatct
     6661 cgtaatattg aagatatacc gtttcccagc cgagcaacgt caatttggat tgaagatctc
     6721 aaacaaagaa ttaagaatct ctatcaaaaa atttttgctg ccaaaaaata ccagtgggtt
     6781 cagttggata caattccaaa gcatgctctt gagaagtttt attatcagaa tttaattccc
     6841 agagaagttc ttgaaaatcc ggcaaaccgg tatctgattt attcgagtca taaggggatt
     6901 ttagtcaatt atcttgacca tctccaaata ttttctatta catcgagttt ggacttaagt
//
LOCUS       DAOSOL010000038         6922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_10998_length_6922_cov_3.470511, whole genome shotgun sequence.
ACCESSION   DAOSOL010000038 DAOSOL010000000
VERSION     DAOSOL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10998_length_6922_cov_3.470511"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..920)
                     /gene="deoC"
                     /locus_tag="PK171_01710"
     CDS             complement(243..920)
                     /gene="deoC"
                     /locus_tag="PK171_01710"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT04953.1"
                     /translation="MKINPKELVKMIDVSLLQSVNTEADIQYLIEKTKKYQFICAFAL
                     PCYSQTLVEAFKDEDIMVGAPIGFPTGAELTEVKIFQAKTLYDIGCDEFDMVMNIGWL
                     KSKQFGKVAKDIQAVYQTIAEKPLKVIVEAMYLTDDELTEACKIVMNSGAEFIKTGTG
                     WATKPTEIRHVEIMAKTIQGRIKMKAAGGIRDYETLCKMHDMGVSRFGVSLSSGIKIV
                     EEARSKP"
     gene            complement(974..2512)
                     /locus_tag="PK171_01715"
     CDS             complement(974..2512)
                     /locus_tag="PK171_01715"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT04954.1"
                     /translation="MSKYIMVWDLGTGGNKASLYDEEGNCLAGVFVPYDTIYPAHGWH
                     EQKPSDWWKAIVHSTRQLLNRTNIKKEEVYCCSISGHSLGAVPLDKQGNLLRKQTPIW
                     SDGRAVEQAQKFFDGFDEIRWYTITGNGFPPPLYSLFKILWYRDVEPEVFKKIDKVIG
                     TKDYINFKLTGRVTTDFSYASGSGVYDLKNWDYSEELIEASGLSASIFPEIVQSTEII
                     GQIRPDSAEELGLHSNVYVVSGGVDNSCMALGSKAFKEGRVYNSLGSSSWIAVSSRNP
                     LLDPITRPYVFAHVVPGFFASALAIISAGSSFRWLRDHITIDLKTQANNQGIDVYELM
                     TAEAAQSQVGSHKLLFNPSLGGGMPIDKNIHIRGAFIGIDLVHTRADMIRAAMEGIAL
                     GLKCCLDKLKEMTRLSEEMLLVGGGSKSELWRQIYADVYNMKVIKTNIDEQAAALGAA
                     ACAAVGTGLWKSFDRIDDLHNLVECITPIPENIEKYQKIYEVYKRVSNCLSDIGNDME
                     KLDV"
     gene            3197..4165
                     /locus_tag="PK171_01720"
     CDS             3197..4165
                     /locus_tag="PK171_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT04955.1"
                     /translation="MSSKNTSFNNNIKAMLGRPEISVLFALLAICVWLSIASPYFLTA
                     RNIVNVFRQFSLIAILAVGQAFIIITGGIDLSVGSLVGFTACFGAMVAKSGASPLLTL
                     IAILGMGAIVGLSNGLLVSKVGLPAFVATLGMMSITRGFSLLITMGVPIHYNPTWISV
                     FGGGYIRNVPVSVLVMLIIVAIGYIFANNTVPGRNVYAVGNSEKAAKLSGIQVEKVKI
                     MVFTITGFLAGICGLILMGQLEGADAFYGNGYELDVIAAAVIGGISMSGGEGNPLGVI
                     VGAALMGVLKNAFVLLAVPGYWQTVVIGVVIIGAVSIDSLRRKGAA"
     gene            4237..5253
                     /locus_tag="PK171_01725"
     CDS             4237..5253
                     /locus_tag="PK171_01725"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT04956.1"
                     /translation="MSKTRLLLVALLGIFCASLFIGIAFAADFVPAAELKAPAGASLY
                     VLPVDQVSEKPLRIATIMVQNNPFGMAVMKGTLFAKEVLKDRNCKVDWISVPDFDPKK
                     FEDAMQNVITAQYDAVTLFGLSEALQPVVDKAMDAGVKVYSFNTEPGLKSKRIAFWGQ
                     DGFTGGQKCGQLLMDAMDGEGKYAIITGSFNVLGHELRRTGARDVIDKNEKMVLVGEF
                     ENQDKAEEAYNVTQNLITSNPDLKGIYVTAGGPFGAAKAIKDAGLTGKLKLVCHDWME
                     ETVAYIRSGEVTACLDQDPFNQGYAPVVSAFNQIVAGIVPEAINWFEGDVATPENVNE
                     KIPQ"
     gene            5330..>6429
                     /locus_tag="PK171_01730"
     CDS             5330..>6429
                     /locus_tag="PK171_01730"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT04957.1"
                     /translation="MSEDIICRIVNVTKVFPGVIALNNVSFDIKKGEIHAIIGENGAG
                     KSTLMNILSGVYYPDEGHIEFEGNVIKFRDPRHAQHTGIAMIHQELSLSRYMSVAENI
                     YQGRMLTKSFGFIDRKKMISECRKYLTTLGVDNIDPRVLVKYLSVSQMQLIEIAKAVS
                     LNAKLLIMDEPTSSLTTGEITLLLKIMKSLKNEGVSILFITHKLEEVLEIADRITVLR
                     DGSYIDTLQKDDATLEKMVSLMVGRNFEKKAHREFIQDYSDREVILEVQNLSSSDRVK
                     NVSFKLYKGEVLGLTGLVGSGRTELLQSIFGMEKMASGEVLINGKEVKINHPADAIKL
                     GLGMIAEDRKEQGMLLKLSVQDNMTIVHLKKLS"
     assembly_gap    6430..6439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6440..6868
                     /locus_tag="PK171_01735"
     CDS             <6440..6868
                     /locus_tag="PK171_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT04958.1"
                     /translation="LSSALQFINKKRSKEISNKYVNLLSIKTPSLKQISKNLSGGNQQ
                     KTIIARWLMNQPKILFMDEPTHGIDVGAKAEIYKLIDELSKMGVSVILLSSELPEVLA
                     LCDRIMVMHHGQLKGILHHQEADQVKIMSFILENKKEIAS"
BASE COUNT         2105 a         1317 c         1292 g         2198 t
ORIGIN      
        1 ttttccaagg ataagcaata aaaaaattaa aaaataaagg caccctcccc gccgcaaagc
       61 gccacccctc caaggaaggg aatttttaaa tatattcccc cattaagtgg ggattcaagg
      121 gagggttgtg catttctcat ttttgtttga tcacttttca ctgtttccgg tattttcagg
      181 atagaaattt ttgccaaaaa aataataaag ggaatcgttt tggtttttaa tataaaaaga
      241 aattacggct tagagcgtgc ttcttcaaca attttaattc ccgaactcaa gctaacccca
      301 aatcgtgaaa cacccatatc atgcatttta caaagtgttt cataatctct aattccacca
      361 gcggctttca tttttattct tccttgaata gttttagcca taatttcaac gtgtcgaatt
      421 tctgttggtt tcgttgccca accagtaccg gtttttataa attcagctcc actgttcata
      481 actattttac aggcttcggt cagctcatca tcagtaagat acatagcttc aacaatgact
      541 ttcaaaggtt tttccgctat tgtttgataa acggcttgaa tatcctttgc tacttttccg
      601 aactgttttg atttaagcca cccaatattc ataaccatgt caaactcgtc acaaccaata
      661 tcataaagag tttttgcttg gaatatcttc acttcggtta actcagctcc agttgggaag
      721 cctattggtg ctccaaccat aatatcttca tccttaaagg cttcgaccaa agtttgagag
      781 taacagggta aagcaaaagc acaaataaat tggtatttct tggtcttttc aattaaatat
      841 tgaatatcgg cttcagtatt cactgactgt aacaaactaa cgtcaatcat ttttaccaat
      901 tcttttgggt taattttcat cgaagaaatt cctcttttct caatattata tttatataat
      961 gaatttattt ttattaaaca tctaactttt ccatatcatt accaatgtca gacaagcaat
     1021 ttgataccct cttatatacc tcataaatct tttgatattt ctcaatattc tcgggaatag
     1081 gagtaataca ttcaacaagg ttatgtagat catcgatccg gtcgaaactc ttccataagc
     1141 cagttccaac tgcagcacag gcagctgccc ctaacgctgc tgcctgttca tcgatattag
     1201 ttttaatgac tttcatattg taaacatcag catagatttg acgccagagc tcgctcttgc
     1261 tccctccacc aaccaataac atttcttcac ttaatctagt catctccttg agtttatcca
     1321 ggcaacattt tagcccaaga gcaatacctt ccattgctgc tcgaatcata tcagctcggg
     1381 tatggactaa atcaattcca ataaaagctc cacgaatatg aatgttttta tcaataggca
     1441 ttccaccacc cagactgggg ttaaataata gcttgtgaga accaacttgc gattgagcag
     1501 cttctgcagt catcaattca tagacatcta tcccctgatt attggcctgg gtttttagat
     1561 caatggtaat gtgatctctt aaccatcgga aacttgaacc tgctgaaatg atcgccaaag
     1621 ctgaagcaaa aaaaccggga acaacatgag caaaaacata gggacgggta attggatcga
     1681 gtaagggatt acgagaagag acggcaatcc aggaagaaga acctaaggaa ttatacactc
     1741 tgccttcttt aaaggctttt gaaccaagag ccatacaaga attatccact cctcccgata
     1801 ccacataaac attggagtgc aaaccaagtt cttctgctga atctggccta atttgaccta
     1861 taatttctgt tgattgaact atttcaggaa aaattgaagc agaaagcccg ctggcttcta
     1921 tcaattcttc ggaataatcc caatttttta ggtcatatac tcccgatccg gaagcatagg
     1981 aaaaatcagt tgtaactcta ccagttaatt taaaattaat ataatctttc gttccaataa
     2041 ctttatcgat ttttttaaag acttccggct ctacatccct ataccataat attttaaaca
     2101 aggaatataa tgggggtgga aatccatttc cggttatcgt ataccatctt atttcatcaa
     2161 aaccatcaaa aaatttttga gcttgttcta ctgctcggcc atcggaccag atcggtgtct
     2221 gtttcctcag taaattcccc tgcttatcta atggaactgc acccaaacta tggcctgaaa
     2281 tgctgcaaca atagacttct tcctttttaa tattggttct atttaataat tgcctggtgc
     2341 tatgaacaat cgctttccac caatcacttg gtttctgttc gtgccaccca tgggcagggt
     2401 aaatcgtatc ataaggaaca aatactcctg ccaggcagtt accttcttca tcgtataaag
     2461 aagccttgtt ccctccggtt cctaaatccc aaaccatgat atattttgac atttttccat
     2521 cccctaagat gttttcaaat aataaaatac ttttagatat tctatcaaga ataatgtgaa
     2581 ttagaagctt tagcaagaca gtcagaatga ggatcacctt ttttgattga ctttttatct
     2641 ccgggaatta taataaggaa agttttctaa cttcattagt aatttaaaat ataaagtttt
     2701 tttaaaagat gtttgattag aagtcttctg aggggggcaa aagtctcttg tgaattactt
     2761 gaagggaagc aaacccattc aaagaacaac gatgagttgt caattcccta atactctttc
     2821 taaaaattgg tcggcttcat ttttttttaa tcctattttt ttatttaact tcgtttttcc
     2881 ttataaattc ctgtcacctc tattcatagt ttcttcagtt ttatcccgaa ataaaggtgt
     2941 gttctgtttt cgtccccttc ttcaacctta caatcatctc actataaaac atctaaatta
     3001 cagcctgttt tcaatcaaaa ctgaattgac atactcaatc ttgttttgaa tttaaaattt
     3061 tataccatat tgattcaggg ttctttggtt acttcgtaaa agaatgaacc aaagtttaaa
     3121 aagcatgtcg ttttttagtt gtttcatagt ttgaattatc taatgaaaaa aaatctaaac
     3181 tacgtagggg agtactatga gctctaaaaa tacttcattc aataataata tcaaggccat
     3241 gttaggaaga ccggaaatta gtgttttgtt cgctctattg gcgatttgtg tctggctatc
     3301 cattgctagc ccgtactttt taaccgcgag aaatattgta aatgttttcc gacaattttc
     3361 actcatagcc atattggctg ttggacaagc ttttatcatt ataaccggtg gaattgacct
     3421 ttcggttgga tctttagtag gttttacagc atgttttggt gctatggttg caaaatctgg
     3481 agcatctcct ttattaacac tcattgcaat tctgggaatg ggagctattg ttggcttatc
     3541 caatggttta ctcgtaagca aagtaggtct tccggctttt gttgcaactt taggtatgat
     3601 gagtatcacc agaggttttt cgcttctcat tactatgggg gttcctattc actataatcc
     3661 cacctggata tcggtatttg gcggtggtta tattcgaaat gttccggttt cagttttagt
     3721 catgctgatt atcgtagcaa ttggttatat attcgctaat aacactgttc caggaagaaa
     3781 tgtttatgca gtaggtaaca gtgaaaaagc ggccaagctt tccggtattc aagtcgaaaa
     3841 agtaaaaatc atggttttta ctataactgg ttttttagcc ggaatatgtg gccttatact
     3901 catgggtcaa cttgagggag cagacgcttt ttacggcaat ggatatgagt tagatgttat
     3961 tgcggcagcg gttattggtg gtattagtat gtccggagga gaaggaaatc ctttgggggt
     4021 tattgtgggt gcagcattaa tgggagttct aaaaaacgcc tttgttttat tggctgttcc
     4081 cggttactgg caaactgtag taattggagt agtaattatt ggagctgttt cgatagatag
     4141 tttgaggaga aaaggagcag cttaaaggta atttcattgg ttttaatagc ttagcttatg
     4201 ctattaaaat aaaaaaaccc ttgaggaggt aagattatgt caaaaaccag gttattatta
     4261 gtagcactac ttggaatttt ctgtgcatca ttgtttatcg gaatcgcttt tgctgcagac
     4321 ttcgttccgg cagctgaact caaagcccca gccggtgcgt ctctctatgt gttacctgtt
     4381 gatcaggtat ctgaaaaacc tttgcgcatt gccaccatca tggttcagaa caatcccttt
     4441 ggaatggcgg ttatgaaggg gactctcttt gccaaagaag tcctcaaaga ccggaactgt
     4501 aaagtcgact ggatctcggt tccagacttt gatcccaaga agtttgaaga cgccatgcaa
     4561 aacgtcatta ccgctcaata tgatgctgtc accctcttcg gtctttccga agctcttcaa
     4621 ccagtagtag ataaagccat ggatgccgga gtaaaagttt actcttttaa tactgaaccc
     4681 ggtttaaagt caaaaagaat tgccttttgg ggtcaagacg gttttaccgg tggtcaaaag
     4741 tgtggacaac tcttaatgga tgccatggat ggagaaggaa agtatgccat cattaccggc
     4801 tcctttaatg tgttaggcca tgaattgaga agaaccggag ctcgagatgt aatcgacaaa
     4861 aacgagaaga tggttctggt tggagaattt gaaaaccagg ataaagctga agaagcctac
     4921 aacgttaccc aaaatttgat tacctctaac ccagatttaa aagggatcta tgtcactgcc
     4981 ggtggtccct ttggtgcagc caaagccatc aaagacgctg ggctgactgg gaaattgaaa
     5041 ttggtctgcc atgactggat ggaagaaact gttgcttata ttcgttcagg tgaagtcact
     5101 gcttgtttgg atcaggatcc tttcaaccaa ggatatgctc cagtggtttc tgctttcaat
     5161 cagatcgtag ctggtatcgt cccagaagca atcaactggt tcgaaggtga tgttgctact
     5221 ccagaaaatg tcaacgagaa gatcccgcaa taaattaatc agattgatta aaaataacaa
     5281 ggcggttcta tgccgccttg ttatttaaca accatggagg aagcgagtaa tgagtgaaga
     5341 tatcatttgt agaattgtaa atgttaccaa ggtattcccg ggtgttattg ctctcaataa
     5401 tgttagtttt gatattaaaa agggtgagat tcatgccatt attggtgaaa acggagcagg
     5461 aaaatcaacc ttgatgaata tcctttcggg tgtctattat cccgatgaag gtcatatcga
     5521 atttgaagga aatgttatca aatttaggga cccaagacat gcccagcata ccggaatagc
     5581 tatgatccat caagaactct ctttgtctcg ttacatgagt gtcgcagaaa atatttatca
     5641 aggcagaatg ttaaccaaat cttttggttt tattgatcgc aaaaaaatga tttcagaatg
     5701 tcggaaatac ttaacaaccc ttggagtaga taatatagat ccgagagttc tggtgaaata
     5761 tcttagtgtg tcgcaaatgc agttaattga aattgccaaa gcagtttcac ttaatgccaa
     5821 actcctcatt atggatgaac cgacttcgtc actcactact ggtgaaataa cgcttcttct
     5881 aaagattatg aaatccttaa aaaatgaagg tgtttcgatt ctttttatca cgcataaact
     5941 cgaagaagtt ttagaaattg ccgatcgaat aaccgtttta cgagatggta gttatataga
     6001 tacacttcaa aaagatgatg ctactttaga aaaaatggtt tcccttatgg tcggtcgtaa
     6061 ttttgaaaaa aaagctcatc gggagtttat acaagactac tccgatcgag aggttatttt
     6121 agaggttcaa aacttgagtt cttcagatcg agtaaaaaat gtcagtttca aactttataa
     6181 aggtgaagtt ttaggtttaa ccggtttagt tggttcaggt cgaaccgaat tactccagag
     6241 cattttcggt atggaaaaga tggcttccgg tgaggtattg attaacggga aagaggttaa
     6301 aatcaaccat ccagccgatg ccataaaact cggtttgggc atgatagccg aagaccgtaa
     6361 agaacaaggc atgttattaa aattatccgt tcaggataac atgaccatcg ttcaccttaa
     6421 aaagctatcn nnnnnnnnnc tatccagtgc attacagttt ataaataaaa agaggtcaaa
     6481 ggaaatctcc aataaatatg ttaatttatt gtcaattaaa acccccagct tgaaacaaat
     6541 ttcgaaaaat ctcagcggtg gaaaccaaca aaaaaccatt attgctcgct ggcttatgaa
     6601 tcaacctaaa atattgttta tggatgaacc tacccacggg attgatgttg gagccaaagc
     6661 agaaatttat aagcttattg atgaactttc aaaaatgggc gtttcggtta ttctcctgtc
     6721 ctcagaactt cctgaagttc tcgccctttg tgaccggatt atggtcatgc accacggtca
     6781 attaaaagga attcttcatc atcaggaagc cgatcaagtc aaaattatga gttttattct
     6841 ggaaaataaa aaagaaatag cttcttgacc atatttctca accaggtagc tttaaatcta
     6901 ttaagaataa ttaaagaggt tt
//
LOCUS       DAOSOL010000039         6907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11029_length_6907_cov_4.894921, whole genome shotgun sequence.
ACCESSION   DAOSOL010000039 DAOSOL010000000
VERSION     DAOSOL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11029_length_6907_cov_4.894921"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            435..1463
                     /locus_tag="PK171_01740"
     CDS             435..1463
                     /locus_tag="PK171_01740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT04959.1"
                     /translation="MVNRKFAIFLFIFIIILQVSLPTFSSEKFIKFSAVGDILLDRGI
                     RMVIGKKGQDYPLKEIDFYLLNRDLVLGNLEGPLSERGEALKKKYIFRGDPSDVAVLK
                     KAGINLISLANNHIMDYGNLALIATIENLKNARLNPFGAGENQEEALKPVIIHKNGLT
                     LSFFASLGYPLQIEKIDPKASGPCQVGLDGFISALQDIRDQVDFIIVSLHWGLEYEAL
                     PHPHQIETAHRLIDNGVDLIIGHHPHVIQGIEKYRGKYILYSLGNFLFDQHGEEEKES
                     IIFSCDFGEGGLLFPNIIPIEIINYQTKSASKEKAEKIITKIHLDSDQGKIFFQKNDD
                     KYYIKEIE"
     gene            complement(1487..1939)
                     /locus_tag="PK171_01745"
     CDS             complement(1487..1939)
                     /locus_tag="PK171_01745"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT04960.1"
                     /translation="MAKKWLTREDSIQYLNEAFYGHLATTSNNQPYLVPVNYVYHQNH
                     LYIHSALQGQKLENIRKNSRICFEVSHPIQFLPGKKPCQFGVHYWSVLVFGQANILDD
                     LNLKNVALNLFIKKYSRDDYLNPMDPDDLQNVAIIDIHIETISGKANN"
     gene            complement(2189..2611)
                     /locus_tag="PK171_01750"
     CDS             complement(2189..2611)
                     /locus_tag="PK171_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOT04961.1"
                     /translation="MSICEHIEFQCPSCNSSQTIEIWRSINTQENPELKKELFEGRIN
                     VFHCLECDFEGSLPVDLLYHDVENQFCVQFFPFEWILDDKFIQRFRFQDGNVVFVPQK
                     DILSLPAYLRNFQITFNMNEMIRYIIFLEKVLPIGKNW"
     gene            complement(2642..4504)
                     /locus_tag="PK171_01755"
     CDS             complement(2642..4504)
                     /locus_tag="PK171_01755"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HOT04962.1"
                     /translation="MFQDFKFTQNPKKRYNINRSKLNQKEERINLALIHTDYRLIKTI
                     DDIQPLLSSLNENIIFAIDTETTGLDPFQNRVRLIQIASPNQPVILVDLWKASHEIIS
                     SLNKILTSPALKIFHNAKFDLKFLLKSGFIVHGPYFDTMLAAQVLSAGQKSQGFKLSD
                     LAREELNIDLPKELQSSDWGNDLSDEQLNYATRDAQVLLEIYPSLLSKLENSNLSATA
                     KLEFECLPAVVDMELSGMLLHTEKWGKLTQELEKNQSDLTATLRQRLSEGFKRIPQLP
                     FMDNQITEPPLLNLDSPKQMLEALQQLGIPLKTTSHEELSQLSRQFPVIQALIDYRKV
                     TKVLQSFASALPKYIHPVTSRIHPDYWQIGTATGRFSCRNPNLQQIPRDQIFRECFIP
                     AAGNILIIGDYSQVELRVAAEISHDQRMIDAFQQNQDIHCLTASLLIGKDIDKVTKEE
                     RQAAKAVNFGLLYAMGARGLKLYAQNSYGVEMTDQQAVNFRKRFFEIYQGIASWQKEL
                     SNSRLKEIRTLNGRLRLWEERPRITELFNTPIQGTAADIVKQAMAMSFPYLNSKDIHL
                     IGTVHDEILLESPQEKGQEISHFLKETMIKAGKKFIQHVPIEVDVVIAKNWSEK"
     gene            4665..4913
                     /locus_tag="PK171_01760"
     CDS             4665..4913
                     /locus_tag="PK171_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04963.1"
                     /translation="MAPDEILTSLNTEGERAREGEGEKKKKTIGLRMTDERHTPLTPL
                     NGGMNSTIPLLGGVPLAAGRVPFSFSKLSTSSKIRILF"
     gene            5081..6190
                     /gene="ddlA"
                     /locus_tag="PK171_01765"
     CDS             5081..6190
                     /gene="ddlA"
                     /locus_tag="PK171_01765"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002525.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOT04964.1"
                     /translation="MESKIKVGILFGGQSVEHEISILSARSIYEAIDKEKYDVVLIGI
                     DKQGGWHFSDEQIFLEYNATHQLRSFTNKSDQQLALIPGRKVDFLYHLSTREKPKPVD
                     VVFPVLHGTNGEDGSIQGFLKLSGIPFVGAGILGSAIGMDKEVTKRLLHDAGISIPHF
                     QVVRQIDSNKIDLLQIQAEFGLPLFIKPANLGSSVGISKVKTIDEIKPAIDKAFLYDQ
                     KILIEECILGREIECSVLGNEHPEASLPGEIKPNHEFYSYQAKYLDENGANFEVPAKL
                     PDNLIKEIQETALKVFQVLEIEGMARVDFFLQEDSKLLVNEVNTIPGFTKISMYPKLW
                     EASGLSYKDLITRLIELAIERAERDQKLKTTYEEY"
     gene            complement(6201..>6907)
                     /locus_tag="PK171_01770"
     CDS             complement(6201..>6907)
                     /locus_tag="PK171_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT04965.1"
                     /translation="GRLAKSIDVLRNRLIRQNEIQQNSLQNISHALKTPVMVIRSYAQ
                     SILDGIFPKGNLEASVLVIDQEAERLQKRNRDLLYLTKLDVLNTINQSQDQYRLDELI
                     IDVMNRFQIRRKDIKWITDLEDISYSGDYEQWTVALENLMDNQVRYAHNTITISLKTQ
                     PNSQILIQVGNDGPSIEEKTMKTLFEKFHPGMKGEFGLGLSITARIIKLHGGTISVAN
                     QEKGVTFSILIPAQKS"
BASE COUNT         2148 a         1220 c         1389 g         2150 t
ORIGIN      
        1 taaatgaaga acgggcataa tacattgtgc ccctaggttt ttaattcttt ggtagcggct
       61 tgatttatca tgcccaattt aaatccgtca ttgcgaggaa ttcaaccact ctttggttgg
      121 atgacatgac aatctctgcc acctcatatg tcattctgag gagtccggtg tcttctgccg
      181 gacgacgtga gaatcttatc ctttaaagta tttttaaaga ataaaaaacc taaaaagatg
      241 agatcctcac gccctcgaaa agcgagggct caggatgacg ccttcggtgt cagatgagat
      301 cctcacgcgg gaaagcaccg ctcaggatga cgccttcgtt gtcagatgag atcctcgagg
      361 cttcgaaaaa cgaagcctcg ggatgacagc gttaagatgt ttatggtaaa tttgtattta
      421 caggattgaa acaaatggta aacaggaaat ttgcaatttt tctattcatt ttcataataa
      481 tcttacaagt atctttaccg actttttcca gtgaaaaatt cattaaattt tccgcagttg
      541 gagatattct tctggatcgc gggataagaa tggttattgg aaaaaagggg caagattatc
      601 ctttaaaaga gatagacttt tatttattaa accgggattt ggtactaggc aacctggaag
      661 gacctctttc tgagcggggt gaagcattaa agaaaaagta tatatttcga ggtgaccctt
      721 cagatgttgc tgtgcttaaa aaagcaggca taaacctaat ttccttagcc aacaaccata
      781 taatggatta tggtaatctt gctttaattg ctaccattga aaaccttaaa aatgccagat
      841 taaatccatt tggtgctgga gagaatcaag aagaagccct taaaccggtt ataatccata
      901 aaaatggatt aactttatct ttctttgcat cccttggtta tccgcttcaa atcgagaaaa
      961 ttgatccgaa ggcctcaggg ccctgccaag ttggattgga tggatttatt tctgcccttc
     1021 aagacatccg cgatcaggtg gactttatca tcgtctctct ccactgggga ttggagtatg
     1081 aagcccttcc ccacccccat caaatcgaaa ctgctcatcg attgattgat aatggtgttg
     1141 atttgattat tggacatcat ccccatgtta ttcaaggaat tgaaaaatat cgggggaagt
     1201 atattttata tagcttggga aattttcttt ttgatcagca tggagaagaa gaaaaagaaa
     1261 gcattatttt tagttgtgat ttcggagagg gaggtttgct atttcccaat attattccga
     1321 ttgaaattat caattatcag acaaaatcag cttctaagga gaaagctgaa aaaattatta
     1381 ctaaaatcca tctggattca gaccagggaa aaatattttt tcagaaaaat gacgataaat
     1441 attatattaa agaaatagaa taacccccat attaacactt ccttttttaa ttatttgcct
     1501 tacccgagat agtttcaata tgtatatcaa ttatagcaac gttctgaaga tcatctggat
     1561 ccatagggtt taaataatca tcgcggctat attttttgat gaagaggttt agggctacgt
     1621 ttttcaaatt caagtcgtca aggatatttg cctgaccaaa aaccaaaaca ctccagtaat
     1681 gtactccaaa ttggcagggt ttttttccgg gtagaaattg gatgggatga ctgacctcga
     1741 aacaaatccg agaattcttt cggatatttt cgagtttttg tccttgaagt gcggagtgga
     1801 tatagagatg attttgatga taaacataat taactggaac cagatagggt tgattattgc
     1861 tcgtggttgc taaatgacca taaaaagctt cattgaggta ttggattgaa tcttctcggg
     1921 tgagccattt cttagccata aaatgcgcac tcctttaaat attttttgct atttttttaa
     1981 tctcaatatt actacggtga atgccaattg atcttgactt tttatgaaat ttgatgttta
     2041 atatgattac cacaggttcc gagaaattgg aaaactttaa gaactgaggg cgcaagtctg
     2101 atgttcctac ggggagttga agagctcgaa ttccccgaag aaaaaattaa agttagaagg
     2161 tttgttggaa tctgtggttt ttttattttc accaattttt accaatcggc aggacttttt
     2221 ccaaaaaaat gatgtatcga atcatttcat tcatattaaa agtaatttga aaattccgaa
     2281 ggtaagctgg aaggcttaaa atatcctttt gagggacaaa aaccacattt ccatcttgga
     2341 agcgaaaacg ttgaataaat ttatcatcca atatccattc aaaagggaaa aactgaacgc
     2401 aaaattgatt ttcaacatca tggtaaagta aatccaccgg aaggcttcct tcgaagtcac
     2461 actcaagaca gtgaaaaacg ttaattcttc cctcaaaaag ctcttttttc aattcagggt
     2521 tttcctgagt attaattgat ctccaaattt caatggtttg tgatgaattg cagctggggc
     2581 attgaaactc aatatgttca caaatcgaca tttccttctc ctttttaaaa ctttaaaaat
     2641 tttacttttc agaccaattt tttgcgataa caacatctac ttctatgggt acatgttgga
     2701 taaatttctt ccctgcttta atcattgttt cttttaaaaa atgagaaatt tcttggcctt
     2761 tttcttgtgg agattcaagt agaatttcat cgtggacggt tccaatgaga tgaatatcct
     2821 tgctattcaa ataagggaaa gacatagcca tagcctgttt cactatatcg gcagccgttc
     2881 cttggattgg agtattaaag agttcagtga ttcggggtcg ttcctcccat agtcgtaatc
     2941 gtccattaag ggttcgaatt tctttaagac gactattact gagttctttt tgccaggaag
     3001 ctatgccttg ataaatttca aagaaacgtt tacggaaatt aactgcctgt tgatcggtca
     3061 tctcgacacc ataagaattt tgagcataga gctttaagcc tcgagctccc atggcatata
     3121 agagaccaaa gttcactgct tttgcggctt gtctttcctc tttggtaact ttatcgatat
     3181 cttttccaat taaaagggaa gcagtcaaac aatggatatc ttggttttgt tgaaaagcat
     3241 cgatcattct ttgatcgtgg cttatttcag cagcaacacg aagttcgacc tgagagtaat
     3301 caccaattat taagatattt cctgcagctg gaataaaaca ttctcgaaaa atctgatctc
     3361 gagggatttg ttggagattg ggatttcgac aagaaaagcg tccggtagca gttccaattt
     3421 gccagtaatc agggtgaatt cgactggtta ctggatggat atacttgggg agagcgctgg
     3481 caaatgattg gagtactttg gttactttac gataatcaat tagagcttga atgactggaa
     3541 attgacggct taattgagat aattcttcat gcgaggtggt ttttaaagga atacccaact
     3601 gttgcaaggc ttccaacatt tgtttagggc tatctaagtt aagaagcggt ggttcagtga
     3661 tttgattatc cataaatgga agctggggga ttctcttaaa tccttcacta agtctttggc
     3721 ggagagttgc tgttaaatcg ctttggtttt tttccagttc ttgggtcaat tttccccatt
     3781 tttcggtatg aagtagcatt ccactgagtt ccatatctac aacggctggg agacactcaa
     3841 attctaattt ggctgtagca gataagtttg agttttccag tttcgaaaga aggcttggat
     3901 aaatttccag caacacctga gcatcacgag tcgcgtaatt taattgttca tcacttaagt
     3961 cgttacccca atcgctgctt tgtaattctt taggaaggtc aatatttaat tcctcacgag
     4021 ccaagtcaga cagcttaaaa ccctggcttt tttgaccagc tgataaaact tgtgcggcta
     4081 acatggtatc aaaataagga ccatgcacaa taaacccgga ttttaacaaa aacttcagat
     4141 caaacttagc gttgtgaaaa attttaagag ctggtgaagt aaggattttg tttaaagagg
     4201 aaataatttc atgagatgct ttccaaagat caaccaaaat tactggttgg tttggagatg
     4261 caatttggat caaccgaact cggttttgaa aaggatcaag tccagtagtt tcggtatcaa
     4321 ttgcaaagat tatgttttca tttaaagatg ataaaagtgg ttgaatatca tcaattgttt
     4381 ttattaagcg ataatcggtg tgtatcaagg ctaaatttat cctttcttct ttttggttta
     4441 atttggatct attgatattg tatctttttt ttgggttttg tgtaaattta aaatcttgaa
     4501 acacttattc tatagtagca cgaggtagca atgaaaatac taatatactt ttttaaataa
     4561 tcgaagacat ttctatctaa ccttatccaa aggagaggaa aaatgaaaaa atcaaaagat
     4621 gagatcctca cggcttcata aagcgaagcc tcaggatgac gccggtggcg ccagatgaga
     4681 tcctcacgtc cttaaatact gagggggaga gggcgcgaga gggtgagggg gagaaaaaga
     4741 aaaagacaat aggacttagg atgaccgatg aaaggcacac ccccctaacc cccctcaatg
     4801 ggggaatgaa ttcaacaatt cccctccttg gaggggtgcc gcttgcggcg gggagggtgc
     4861 ctttcagttt ctcaaagctc agcacatcga gcaaaatccg gatacttttc taagggcaaa
     4921 atgagaagtc tcagttagaa aacaattaaa ggcacacccc cctaaacccc ctcaatgggg
     4981 gaattcattt gataatatta acaggataga taatacagtt ccataatatg atatataatt
     5041 cattgaatac tttttgataa taaaaggagt taatgcccaa ttggaatcta aaattaaagt
     5101 aggaatatta tttggtggtc agtcggtgga gcatgaaata tcaattttgt cagcgcggag
     5161 catatatgaa gccattgaca aggaaaaata tgatgttgta ctgattggta ttgacaagca
     5221 aggagggtgg cactttagtg atgaacaaat ttttcttgaa tataatgcta cccatcaatt
     5281 aagatcattt accaataaga gcgatcaaca attagcgtta atacctggca gaaaggtcga
     5341 ttttttatac catctatcaa cccgggaaaa accaaaaccc gttgatgtcg tatttccagt
     5401 tttacatggt accaacggtg aggatggatc aatccaaggt tttttaaagt tatccgggat
     5461 tccctttgtt ggtgccggaa tacttggatc agctattggt atggataaag aagtaacaaa
     5521 acgcctgctc catgatgccg gcatctctat cccccatttt caagttgtgc gtcagattga
     5581 tagcaataaa attgacctgc ttcaaatcca ggctgagttc ggtcttcctc tttttataaa
     5641 accagctaat ctgggatctt cagtgggaat tagtaaggta aaaaccattg atgaaatcaa
     5701 accagccatc gataaagcct ttctttatga tcaaaaaata ttaatagaag aatgcatact
     5761 gggcagagaa atcgaatgtt cagtactggg aaacgaacat ccggaagcgt ctcttccagg
     5821 tgaaatcaaa cccaaccacg aattttattc ctaccaagca aaatatctag atgagaatgg
     5881 tgcaaatttt gaggttccag ccaagttgcc tgataattta ataaaagaaa ttcaagaaac
     5941 ggcgttaaag gtcttccaag tattagaaat cgaaggaatg gcacgagttg actttttctt
     6001 gcaagaagat agtaaactct tggtaaatga ggtgaacacc attcctgggt ttaccaagat
     6061 cagtatgtat cccaagcttt gggaagcaag tggtttatct tataaagact taataactcg
     6121 tctcatagag ctagctattg agcgggcaga acgggatcaa aaattaaaaa ccacctatga
     6181 agagtattaa aattcactcg tcatgatttt tgagctggaa ttaaaataga gaaggtaacg
     6241 cccttttcct gattagcaac ggatatggta ccgccatgga gtttaatgat tcttgcagtt
     6301 atagatagac cgagcccaaa ctcgcctttc atgccaggat ggaatttctc aaaaagtgtt
     6361 ttcatagtct tttcttcaat agaaggtcca tcatttccaa cctggatgag aatttgggag
     6421 ttgggctgag tcttgagtga aattgttatg gtattatgag catatcggac ttgattatcc
     6481 attaaatttt ccagggctac agtccactgt tcataatcac cggaataact tatatcctca
     6541 agatctgtaa tccacttgat atcctttctt cttatttgga aacggttcat aacatctatg
     6601 atgagttcgt caagtcgata ctgatcttga gattgattga tggtgttaag gacgtcaagt
     6661 ttagttaaat ataaaagatc ccgatttctt ttttgcaaac gttcagcttc ttgatcgatt
     6721 actaaaaccg aagcctccag atttccttta ggaaagatgc catcaagaat tgactgagcg
     6781 tagctcctta tgaccattac tggagttttg agggcgtggg atatgttttg caaagaattt
     6841 tgttgaatct cattttgtct aattaaacga tttcttaaaa catcaattga cttagccaaa
     6901 cgaccaa
//
LOCUS       DAOSOL010000040         6813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11228_length_6813_cov_4.279225, whole genome shotgun sequence.
ACCESSION   DAOSOL010000040 DAOSOL010000000
VERSION     DAOSOL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11228_length_6813_cov_4.279225"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2753)
                     /locus_tag="PK171_01775"
     CDS             complement(<1..2753)
                     /locus_tag="PK171_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT04966.1"
                     /translation="MPFEVFHYNRLSDVKEYLGQLTTQVINPIIIVPSLSDKSEFREM
                     DPFEESKIFQWNDLYNEIHTLLKNQVSIPPKRKEVDQSVNWLLIHNLWKELNEKTETI
                     DIPEGLRRIECVTHILESIRELLREEVSWSDFNRFMSCNDCFDDSLCNQIQDPTSWLC
                     WIYRRYLKVFERYQLSDNLQIPGLTADLIRKSWYNEELKNWINKNTFIFIGFFQFTPG
                     QRQLLHSLNSNYGQVYIIKPWCGIPQFDENFPLQLDHSKLPLPINNPVSIGEIAAGDA
                     RHQYETIARELVLWSAEKGQLSQWGQFPGWDDIATIIPIQRRKTFQEVLQKYRIPFQF
                     LQGFSAKESPLWGILKRLRSAGQSYWPEEETLHLLTDPLLGETDISVKEAYHLSPRGI
                     EGWRDFLKDSPQSLLKFDQLVKSWRIIESCRTPQEVYHVLHRIFTKTFNISNNASKLS
                     GLDPTRDELVALLTAFLVEIEQKILYFSEDETLASFVQQINFASTNEVYGFLETWINH
                     ATLRPPLVEKGSLRVYLSSPPALTEAPVMIITDIISAFWPGSFLNTPFLEDELKKKWN
                     QNPGLNQATLPTIHHKRDEKRALFRRLIATGLQYTIVTYPLQDEAGRPTSLSPYYDEI
                     FTGDDPWAEKVVFFKRPLSAILPQTVEPYLKPVEIPEDVPLIARSLPWLPNQISKIPP
                     IPISELDNWVECPFLFYLKNFYKLTEPIPPGFDPRRGGIVLHDLWRKVWDKYLEGKST
                     SLRELVLSLWPEVVQRIYPELLTIFISDELRLRLDALRMADYQEHMESFINHYRIEEK
                     NEFVLPPYEINQLSFTGRCDRLIGLKEGYWLIVDYKSGKSNYARTSLQLAGYSQVLTD
                     NGYPVIGYVYLCHGDGKAIGAFENNIFVPYGDKLRNAILKSNRSGKILSVEMELARQK
                     MEEL"
     gene            3013..4929
                     /locus_tag="PK171_01780"
     CDS             3013..4929
                     /locus_tag="PK171_01780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT04967.1"
                     /translation="MNRNTLNCLLLVFIFISLISFGNLAIAYSEEIPLIPLRDFFRNP
                     EKAGYSLSPDGEYFAFLAPWENRLNIFIQKFSETEAKRITSVTDRDLVGYFWASNERI
                     VYARDQAGEENFHLFAVNIDGSNPVDLTPFEGVRAGVIDDLRDNDQDMIISLNKRDPR
                     FYDAYRINIETGELKLIGENPGNIIGWLTDHEGLLRLAITSDGVNTSILYRDNESQSF
                     QTILTTDFKETLTPVSFTYDNQCLYAFSNLGRDKAALVVFDPKTQKELELIYENSYVD
                     VSGLLISDKRKKLIAVTYLTDKLRYHFFDEEVESIFQDLTKKIPGYELALSSVNQDEN
                     RFIIRTYSDKSLGSYFLYDVETSNLQKIADISPWIDEKYMADMEPISYNSQDGLTIHG
                     YLTLPKGVEPKNLPVVINPHGGPWYRDSWGYNAEVQFLANRGYAVLQMNFRGSTGYGR
                     TFWEAGFKQWGRKMQDDITDGVQWLIDQGIADLKRIGIYGGSYGGYATLAGITLTPDL
                     YAAAVDYVGISNIFTFLEAIPPYWEPLRQQFYEMIGDPSKDKELLESVSPLFLVNQIK
                     TPLFIAQGANDPRVKKSESDQIVAALEKRGIKVQYMVKENEGHGFANEENRFDFYRAM
                     EEFLSEHLGGKVEP"
     gene            4997..5167
                     /locus_tag="PK171_01785"
     CDS             4997..5167
                     /locus_tag="PK171_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04968.1"
                     /translation="MADENHDWSLSGIAFAGCLFIGMGLGILFKHTWAGIMMGLGVGF
                     IVMAILRWKNKV"
     gene            5817..>6813
                     /locus_tag="PK171_01790"
     CDS             5817..>6813
                     /locus_tag="PK171_01790"
                     /inference="COORDINATES: protein motif:HMM:NF027591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4919 domain-containing protein"
                     /protein_id="HOT04969.1"
                     /translation="MSYRFRFLHFCSFLLIFCIVFLIFVDQGNAQDNPTNYYQTLLDR
                     LKNGDTSINFAELRVLYAQLPTYNPYKIMQDLKEKEDQMWQEYKSGNYDKALEIGTSI
                     LEMNYLRIMTHYIFSEVYGKLGDTQKQKFHEDVFFGLVDSIIQSGDGKSPETAMTVIE
                     IREEYDVLDVLGFEQESQTLVEKDGKRFDFLVAKNSETGETRDFYFNIDLFYDKAIEI
                     DEESSLTPKTTSQVEPTSVIESGKFLDNLFIATLVTGTKQEIESFIQDQKVDFKLRDK
                     DGNTALMIAAMGNSNHDVINYLLSLGIDINAKNNKGDTALIKAASLTTEPKIIEAL"
BASE COUNT         2087 a         1273 c         1273 g         2180 t
ORIGIN      
        1 agttcttcca ttttttgtcg ggcaagttcc atttcgacgc ttaaaatttt accggaacga
       61 ttcgacttta agattgcatt cctcagctta tcgccatagg ggacaaaaat gttattttca
      121 aaagcaccaa ttgctttccc gtcaccatgg cagaggtaaa catagccaat caccggatat
      181 ccattgtcgg ttaaaacttg gctgtaacca gctaactgca atgaggtcct cgcataattt
      241 gattttccac ttttataatc tacgatcaac cagtatcctt cttttaaacc gatcaggcgg
      301 tcgcaacggc cagtaaaaga aagttgattt atttcatacg gggggagaac aaattcattt
      361 ttttcttcaa ttcggtaatg atttataaaa ctttccatat gttcctgata gtcggccatt
      421 cttaaagcat caagccttaa acgaagttca tcgctaataa aaatcgttaa gagctctggg
      481 tagatccgtt ggacaacttc tggccaaagg gacaacacca gttcccgaag gctggttgat
      541 tttccttcta aatatttatc ccacactttt ctccacaagt catgcaagac tattccaccc
      601 cgacggggat caaaaccggg aggaatcggt tcagttaatt tatagaaatt tttgaggtaa
      661 aacaaaaaag ggcattctac ccagttgtcc aactcactga tcggtattgg aggtattttt
      721 gaaatttgat tgggtaacca aggaagacta cgagcaatca aaggtacatc ctctggtatt
      781 tccaccggtt ttaaataagg ttcaacggtt tgaggaagaa tagcactcaa tggtcgttta
      841 aagaaaacca ctttttctgc ccaaggatca tcacccgtaa atatttcatc ataataagga
      901 gaaagcgatg ttggtcgtcc tgcttcatcc tgcaatgggt aggtcacaat ggtatattgt
      961 aatccagtgg caatgagtct tcgaaataaa gctcgttttt catctctctt atgatgaatg
     1021 gtaggaagag tagcctgatt caatcccgga ttttgattcc actttttttt cagttcgtct
     1081 tccaaaaaag gggtattcaa aaagcttccg ggccagaaag ctgaaattat atcagtaata
     1141 atcatcactg gtgcttcggt caacgctggg ggagaactga gatacactct caaggatccc
     1201 ttttcgacaa gtggtggacg gagggtggcg tgattaatcc atgtctctaa aaaaccataa
     1261 acttcattag ttgaagcaaa atttatctgt tgaacaaagg atgccaatgt ctcatcttca
     1321 gaaaaataaa gaattttttg ctcaatttca accaaaaaag ctgttagtaa agcaaccaac
     1381 tcatctcgag taggatctaa cccagaaagt tttgaggcat tattagaaat attgaaagtt
     1441 ttggtaaaaa ttcggtgaag gacatgataa acctcttgtg gtgttcggca ggattcaatt
     1501 attcgccaag atttgaccag ctgatcaaat ttcaataaag attgagggga atcttttaag
     1561 aaatctctcc atccttcaat cccacggggt gataagtgat aagcctcttt gacagaaata
     1621 tccgtttctc ctaataatgg gtcggttaga agatgaagag tttcttcttc tggccaataa
     1681 ctttgaccag cacttcgtaa tcgtttaaga attccccaca agggggattc cttggccgaa
     1741 aatccttgca ggaattgaaa gggaatccgg tatttctgta aaacttcttg aaaagttttt
     1801 cttctttgga ttgggatgat cgtcgcgata tcatcccaac cggggaattg gccccattga
     1861 gaaagttgac ctttctctgc cgaccataaa accaactcac gagcaatggt ttcatactga
     1921 tggcgggcat ctccagctgc aatttcacca attgaaactg gattgtttat cggtagggga
     1981 agtttagaat gatctagttg caaaggaaaa ttttcatcaa attgagggat tccacaccaa
     2041 ggcttgataa tataaacctg accgtaattc gaattcaaac tatgcaataa ttgcctttgc
     2101 cccggagtga attgaaaaaa accaataaaa ataaatgtat ttttattaat ccaatttttt
     2161 aactcttcat tgtaccagga ttttcgtatc agatcggcag ttaatcccgg tatttgaagg
     2221 ttatcgctga gctgataccg ttcaaatacc ttgaggtatc tccggtaaat ccaacataac
     2281 caactggttg gatcctgaat ttgattacaa agggaatcat caaaacaatc gttacaactc
     2341 ataaagcgat tgaaatcgct ccaactcact tcttcgcgaa ggagttcacg gatgctttct
     2401 aaaatatgag tgacacattc tattcttctt aatccctctg gaatatcgat ggtttcagtt
     2461 ttttcattca attctttcca gagattatga atcaacaacc aatttacact ctgatcgact
     2521 tctttccttt tcggcggtat actcacctgg tttttcaaaa gagtatgaat ttcattatat
     2581 aagtcattcc attgaaaaat tttggattct tcaaaaggat ccatttcacg aaattcactt
     2641 ttatctgata aagaaggaac tatgataatc gggttgatta cttgagtagt taattgacca
     2701 agatattctt tgacatcact taaacgatta taatgaaata cttcaaacgg caatttagaa
     2761 aaccttcctc atgctatttt taaactttat actcctttgt aatcactctt ttcccttctc
     2821 aaagatttat tcactttcca tctataatct attctattat aatggaaatt agatttcttt
     2881 attattatcg acttggttag atggtcattt ttatcaacca ttcaaacaag aaaattaaac
     2941 agaaaaagta tcaacatttt tcttaaagtt taattactaa ccaattaaat actatttgag
     3001 gtgtttttaa aaatgaatag aaatacttta aattgtttgc tgctggtttt tattttcatt
     3061 tctctaataa gcttcggaaa tttagcaata gcttattcag aagaaatacc actcattccc
     3121 ctgcgagatt tctttcggaa tcccgagaaa gctggttatt ctctttctcc tgatggtgaa
     3181 tatttcgctt ttctcgctcc ctgggaaaat cggttaaata tttttatcca aaagtttagc
     3241 gaaactgaag ccaagcggat cacgtcagtt accgatcgag atttagtggg atatttttgg
     3301 gcaagcaatg aaagaatcgt ctatgctcgg gatcaagctg gagaagaaaa ttttcattta
     3361 tttgccgtga atattgatgg ttcaaatcct gttgatttaa ctccctttga gggagttcgt
     3421 gccggtgtaa ttgacgattt aagggacaac gatcaagaca tgattatttc tctcaacaag
     3481 agagatccaa ggttttacga cgcctatcga attaatattg aaactggtga attgaaatta
     3541 atcggcgaaa acccgggtaa tatcatcggt tggttaaccg atcatgaagg cctactgcga
     3601 ctcgccataa ccagtgatgg agttaacacc agtattcttt accgagataa cgagtcacaa
     3661 tccttccaaa caattttgac tactgatttt aaagaaaccc tcactccagt atcctttacc
     3721 tatgataatc aatgtttgta tgctttttcc aatttaggcc gcgataaagc agctctggta
     3781 gtttttgatc ctaaaactca aaaagaactc gaactcattt atgaaaattc ctatgttgat
     3841 gtaagcggat tattaatatc agataagaga aaaaagctaa ttgctgttac ctatttaact
     3901 gataaactcc gttatcattt ttttgatgaa gaagttgaat ccatttttca agaccttacc
     3961 aagaaaatcc ctggatatga attggctctc tcaagtgtga atcaagacga aaatcgcttt
     4021 ataataagga cctatagcga taaatcattg gggtcctact ttctctatga tgtcgaaacc
     4081 agtaatcttc aaaagattgc cgatatcagc ccctggattg atgaaaagta tatggctgat
     4141 atggaaccga tttcttataa ttcacaagat ggcttaacta ttcatggata tctcactctc
     4201 cccaagggcg ttgaaccaaa aaatttacca gtagtgataa atccccacgg tggtccatgg
     4261 tatcgagaca gttggggata taatgccgaa gttcaatttt tagccaaccg aggttatgct
     4321 gtactacaaa tgaattttcg cggctccact gggtatgggc gtacattttg ggaagccggt
     4381 tttaaacagt ggggtagaaa aatgcaggat gatattaccg atggagttca gtggcttatc
     4441 gatcagggta ttgccgatct aaagcgtatc ggtatttatg ggggttccta tggtggttac
     4501 gctaccttag ctggaattac tctcactcct gatctttacg ctgctgcggt tgattatgtt
     4561 ggaatttcaa atatatttac ctttctcgaa gctattcctc cctactggga gccgttacgc
     4621 cagcaatttt atgaaatgat cggcgatccc agtaaagata aggagctttt ggagtcggtt
     4681 tctccattat ttttagtaaa ccaaatcaaa actccacttt ttattgccca aggtgctaat
     4741 gatccccggg taaaaaaatc cgaatccgat caaattgttg ccgcgttgga aaaaagagga
     4801 attaaagttc aatatatggt gaaagaaaat gaaggtcatg gatttgccaa cgaagagaat
     4861 cgatttgatt tctatcgagc catggaagaa ttcttatcag aacatttagg tggaaaagtt
     4921 gaaccctaat gttggccaaa aagttttttg atttccatga ataattgagt ctaaaaaaat
     4981 tgaaggaggg attttcatgg ccgatgaaaa ccatgattgg tcattgagtg gcattgcttt
     5041 tgccggatgt ctttttattg gaatgggttt gggaattctt tttaaacata cctgggcagg
     5101 aattatgatg ggacttggag ttggttttat cgttatggct attttaaggt ggaaaaataa
     5161 agtttgagtt tcttgaggtg atgtttttcc accaatgaaa ttctctggtt tctaaatgta
     5221 taggaattta tgcaataaat ttaaagtggg gatttacccc cacttgtcac cttcttccat
     5281 ttctttttcc tctcccctgc taggagagtc actcttttct ttcctcgccc ctcagtacgc
     5341 taggtcaggg tgaggaggta actattttca tcctcatctg ttatcaccaa agttgcatga
     5401 aagtctattc tgataactta cgggcatgat aaatcaagcc ccaacaaaag atttttaaaa
     5461 agcgtcatcc tgaggggtgc ttttccgcgt gagaatctca tcttttaatt ttatctttta
     5521 tcctttaatt ctttttttat tcaccccctc ccccattctt cccatcgctc cctcagcctt
     5581 tatcgtgtct gattaatgta gcggacctgc catggcatgt cgtatcttgg attttctcta
     5641 tcatttagtg ctgctaagca acacaatggt cgctcctgtt aaaaattatt aaaaattttt
     5701 ttgaaagaag gttccttcat tctggttttg atcagattga atgatacaat aaatgttgta
     5761 tttatagatt tttaaacatg aaaagttata gctattgact ataaggaggg attacaatgt
     5821 cctatcgatt cagatttttg cacttttgtt cttttttatt gatcttttgt atagtttttc
     5881 ttatttttgt tgaccaggga aatgctcaag ataatccaac caattactat caaactcttc
     5941 ttgatcgtct gaaaaatggc gataccagta tcaattttgc agaattacga gtactttacg
     6001 ctcagctccc cacctataat ccctataaaa taatgcagga tcttaaggaa aaagaggatc
     6061 aaatgtggca agagtataaa agcggcaact atgataaagc cttagaaata ggaacctcga
     6121 ttctggaaat gaactattta agaattatga cccattatat tttttctgaa gtctatggaa
     6181 aattaggtga tacccaaaaa caaaaattcc acgaagatgt cttttttggt ttagttgatt
     6241 ctattattca atccggagat ggaaaaagtc ctgaaacagc tatgacagtc atcgagatca
     6301 gagaagaata cgatgttctt gatgttttgg gttttgaaca agaatctcaa actttagtag
     6361 aaaaagatgg gaaaaggttt gatttcctgg ttgctaaaaa ttcagaaact ggagaaactc
     6421 gtgattttta ttttaatata gacctctttt acgataaagc cattgaaatt gatgaagaaa
     6481 gcagccttac tccaaaaact acttctcaag tagaaccaac ttctgttata gaatcgggaa
     6541 aatttctaga taatttattt atcgccacct tagtcaccgg aaccaagcag gaaatcgagt
     6601 cattcatcca agatcaaaaa gttgacttta aacttcgtga taaagatggg aacacagctc
     6661 ttatgattgc tgctatgggg aatagcaacc atgatgtgat aaattatttg cttagcttag
     6721 ggattgatat taatgccaaa aacaataaag gcgataccgc tctcatcaag gctgccagct
     6781 taaccaccga accaaaaatt attgaggctt tat
//
LOCUS       DAOSOL010000041         6786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11285_length_6786_cov_3.914723, whole genome shotgun sequence.
ACCESSION   DAOSOL010000041 DAOSOL010000000
VERSION     DAOSOL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11285_length_6786_cov_3.914723"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="PK171_01795"
     CDS             complement(<1..171)
                     /locus_tag="PK171_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT04970.1"
                     /translation="MSFGKEVLRLENISKSFPAVKALDQVNLSVKAGEVRGLVGENGA
                     GKSTLIKIVTGAY"
     gene            complement(447..1427)
                     /locus_tag="PK171_01800"
     CDS             complement(447..1427)
                     /locus_tag="PK171_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT04971.1"
                     /translation="MRKIMLLLMVIFVLLGTAFCYAADVEPYDVAFIVKATDSDFWQY
                     TIVGAKNAEFDLKGLVKITVYGPPSEADIDEQVAILENVVNTKPDAIVIASTSSEATS
                     LVLDNAYKQGIKIVLIDNFVYDTGIHSFLATNNKVGGALAADNLVESLKAAGKPLEGK
                     ISLISAMAGVQVLVDRDDGFAARLKEIAPGLEVLPTRYVDNDIAVAANAAEDLLTANP
                     DLLGFFADNNHTGDGVARVIEERSLQDKIVAVAYDSDPQEIDALRSGALKALIVQDPH
                     GMGYKGVMFAFMAINGEPLPQYYDTGVYVITKENLDDSMWILDPFSRKKY"
     gene            2057..3361
                     /locus_tag="PK171_01805"
     CDS             2057..3361
                     /locus_tag="PK171_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT04972.1"
                     /translation="MKKFFWFTLLIVIVTLSFGTSLLFAAEPDFSKVPTAPKPEKLTV
                     VLFEPVEQKAAIEVSKLFEEKFGIKVEINAIPWSNLHEKIIADLTAKTGTYDIIFIPG
                     LWSLEFIYADYIEPLNQFWDNPNLPRFDIEDYPASVVNLVSQDGNIYWIPHHGTTQIL
                     FYRKDLFEAEGLKPPETYDELFEIAAKFTNNPKYPDVWGFATTPAQGEFASSTWSTWL
                     WSWGGDYFDENWNPIFNNEIGVASLEAYAEAVKKFSPPDSLNWGNDDSGAAFQQGRLA
                     MLQMWPYLGAAMEDPSQSKVVGKVGYAPLPKKELLFPRLGSWGGTISKFSKNKEWAYM
                     WLAFYNSKENTNNILVPSGVAVDRLSTVEELKDKILWQEAVNLSFEHTKERPGIPEIT
                     PIIDVWGLAVSKVVTGQADAKSALDEAAVKVRKILEDAGYYD"
     gene            3468..4403
                     /locus_tag="PK171_01810"
     CDS             3468..4403
                     /locus_tag="PK171_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT04973.1"
                     /translation="MRFKAHNPAPSQLKRSSFQYSEKSTPFYMLAPVLASLAVLVYFP
                     LCNTIGLSFLRYFWNKPQQPIRWVGLKNYIDVFTDSLFWQSLNNTIIFMVVSTCLTLL
                     IGLGIALLISRDFPGKNIFISLILLPMMISPVAAALSWKFLYNGEWGAVNYFVGLLGI
                     PKQAWLADPKLALPAIIVTDIWLNAPFVFLVIYTSLQALPQEPIEAARIDGGTGWQIL
                     SKVILPLLAPNFLLILVIRLMDTFRVFDFIYVMTSGGPGGKTETIGFLSYERTFRHFN
                     MGQGSVIAIFLLIAVLSLSWYFIKTLRLQLQEQAK"
     gene            4457..5296
                     /locus_tag="PK171_01815"
     CDS             4457..5296
                     /locus_tag="PK171_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT04974.1"
                     /translation="MRKPVTYKKILFWILFYFVLICVILFFIFPIFWIATTSLKQRVD
                     VYTEKPVLIFRATWENYQRVFELQDFSRYLLNSLIVAGSVTLIIMYFGALAAYALTRW
                     RFAGYNWISLAIISARMFPPIALLPSFFLFASRLGLVDTRQVLVFAHATYGLPFIVFM
                     LAGFFRQIPRDLDDAALIDGCSRWQLFNRIMLPSVAPGLAAAAILSFVWSWNEFLFAL
                     VLTRASTKTLPVVISEFIGFVQVDWQGLTAMATILMIPSIIITFATQRYLVRGLTAGA
                     VKY"
     gene            5315..6463
                     /locus_tag="PK171_01820"
     CDS             5315..6463
                     /locus_tag="PK171_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04975.1"
                     /translation="MKQELWETVKKYYLHWWNNDFIGRIPFWVSAPKDDPRSQKALFG
                     KHLWILEKEKFGTEKIIKNAREILRATFYGGLAFPCYFPNFGTDVFSAYLGAEMEFSD
                     LFPPVATGPSFIKEDVISVSWAKWGNPVLTDYSNLSRLQINEDNPYWQKTREFIRYAG
                     QLSQGEFFIGATDIHPSMDSLAVLRGSPQNLSLDLVENSQGVKKAMELLWKVWVRVYE
                     DTYYNLIKNQQEGTTAWINLWAPGKFYPVENDLSLMISTDMYREFFLEELVKEINYLD
                     YSIYHLDGKDALHHLDMILDIPKLNAIQWVAGASESVAGVAKWIPLYKKIQANGKAII
                     VYCNPDEVTLVIDSLKPEGLLISVNCETEKEARELLNNYGWEGFYWWE"
BASE COUNT         1904 a         1505 c         1317 g         2060 t
ORIGIN      
        1 ataagctcca gtaacaatct taatgagggt tgattttcct gctccatttt ctccaaccag
       61 ccctcgtacc tcacctgctt tcactgatag atttacctga tccaaagctt tcactgcagg
      121 aaaagattta ctaatattct caagtcttaa gacctctttt ccgaaagaca taagacctct
      181 cctttggttt gaaagtgttt actacagctt tttctgaaca gttcattttg aatggctaaa
      241 taatataccc ttccttttta taccagtcaa aaagtaaaat taattgtcat tagaatccca
      301 acaccaacaa tataaagcca aattccaaaa agctatgagt ttacgaaagc tatagaaacc
      361 tctcttaaaa cgccactcta tttttggcat taagatagaa atgaaatagc gggtggcaat
      421 aaccacccgc tatttcattt aacctattaa tatttttttc tggagaaagg atcaagaatc
      481 cacatagaat catcaaggtt ttccttggtg attacgtaaa ctccagtatc ataatactga
      541 ggcagaggtt caccatttat tgccataaat gcgaacatga ctcccttata ccccatgccg
      601 tgtggatcct gaacaatgag ggctttcaaa gctccgctcc gcaaggcatc aatttcctgc
      661 ggatcagagt cataggcaac agcaacaatc ttatcttgca agctccgttc ctcaattacc
      721 cttgcaacac catcaccagt atggttgtta tcggcaaaga agccaagtaa atcaggattt
      781 gcggttaaaa ggtcctcggc agcattggca gcgacagcaa tatcgttatc tacgtatcgg
      841 gtaggaagaa cttcaagccc tggggctatc tctttaagtc tggcagcaaa cccatcatcc
      901 ctatcaacca gaacctgtac tccagccatg gcactgatga ggctaatttt cccttctaga
      961 ggctttccag cagctttgag agattcaacc aggttatcag cagccagggc tcctcctact
     1021 ttgttgttgg ttgctaagaa agagtgaatt cctgtgtcgt aaacaaaatt gtcaatcaag
     1081 acgattttta taccctgttt gtatgcatta tcaagaacca aagatgtagc ttctgaacta
     1141 gttgatgcaa taacaatggc gtccggcttg gtgttgacaa cattctccaa gatagctact
     1201 tgctcatcaa tatcagcttc cgatggcgga ccatagacag ttattttaac caaaccttta
     1261 aggtcaaatt ccgcattttt tgcaccaaca atggtatatt gccagaaatc agagtcagtt
     1321 gccttgacaa taaaagctac gtcatagggt tcgacatcgg cagcatagca aaaggcagtt
     1381 cccaagagga caaatatcac cattagtaat aacataattt tacgcatttt aggaccctcc
     1441 tcatattgtg tttagtagta aatgttttta aatacaactt ccctccttcc cttaccaatc
     1501 gatagctatc acctccttct aattgtttga taaatatttc taaccaatac tacctttaaa
     1561 attattatac aattttacat ttatagagtc caataattaa tatcaaatta tagtgattag
     1621 ggcaaacccc tcttttaagg ttttttaatt tttctaattt ttcaaatctc cctaaccctc
     1681 tttgctaaag ggaaagattg atacctgaat aggtattttt cattttcgtt tggttaaaaa
     1741 taagtggctt agggtctatc ttgaaaatac cggaataatt tttataggta actcatattg
     1801 agcaaccaac cagaatctca ttccgggttt tcaccctcat cctcaccttc tcccatcaag
     1861 ggagaaggaa ttataaaaat gcgtcatctt gaaccctctt ctcttctcct ttttctcccc
     1921 ctcgccccct caccccatca gctctttcat cctcatctgg tgctatgaaa gtagcatgaa
     1981 catctattca tgaaatcatt gaaaatgaga aaggataaat atatactatc tttattaaag
     2041 caaaaggagg gaaaaaatga aaaagttttt ttggtttact ttacttattg ttatagttac
     2101 tctttcattc ggaacaagcc tactcttcgc tgccgaaccc gattttagca aagtcccgac
     2161 tgcaccaaaa ccagagaaat taaccgtagt tttatttgaa cccgtcgaac aaaaagctgc
     2221 cattgaggtc agcaagcttt ttgaggagaa attcggaata aaagttgaaa tcaatgcgat
     2281 accctggtcg aatcttcatg aaaaaatcat tgccgatctc actgccaaga caggaaccta
     2341 tgacatcatc tttatcccgg gcttatggtc actcgagttt atttatgctg actacattga
     2401 accacttaac caattttggg ataaccccaa cttaccgagg tttgatattg aagattatcc
     2461 tgccagtgtt gtgaatttgg tttcccagga tggaaatatt tactggatcc ctcatcatgg
     2521 aaccacccaa atccttttct atcggaaaga cctttttgaa gccgaaggat tgaaaccacc
     2581 ggaaacttat gatgagttgt ttgaaatcgc cgcaaagttt accaataatc ccaaatatcc
     2641 tgatgtctgg ggttttgcaa ctactcctgc gcagggtgaa ttcgcttcca gtacctggag
     2701 tacttggctc tggtcatggg gtggagacta cttcgatgaa aattggaatc caatttttaa
     2761 taacgaaatt ggtgtggcgt cgttagaagc ctatgccgaa gcagtcaaga aattttcacc
     2821 tcccgactca ctcaactggg gtaatgacga tagtggtgct gccttccaac aaggcagatt
     2881 agctatgctt caaatgtggc cgtatttagg agcagccatg gaagatccca gccaatcgaa
     2941 agtggtgggg aaagttggat atgccccact tccgaaaaag gaacttcttt tcccaaggtt
     3001 aggatcatgg ggtggaacaa tatctaaatt ctccaagaac aaagaatggg cttatatgtg
     3061 gttagctttt tataatagca aagaaaatac taataatatt ttagtaccct ctggagttgc
     3121 tgttgaccga ttatcaacgg ttgaagaatt aaaagacaaa atactctggc aagaagctgt
     3181 caacttaagc tttgaacaca ccaaggaaag gccgggtatc cctgaaatta ctccaatcat
     3241 cgatgtctgg ggcctagcag tttcgaaagt ggttaccgga caagccgatg ctaaatcggc
     3301 tctggatgag gctgcggtaa aagttcgtaa aatcttagaa gatgctgggt actacgatta
     3361 aaaacaataa ttgcgagatg gaggactccc tgtctccatc tcgcttctta atgtcctaaa
     3421 accttactca tttaaacctt attaatgcag aaagggcgaa tcaagcgatg cggtttaaag
     3481 cacataatcc agctccttcc cagctaaaaa gaagcagttt ccaatatagc gaaaaatcta
     3541 ctccttttta tatgctcgca ccggttttag caagcttggc ggttttggtt tattttcctt
     3601 tgtgtaatac aattggtctt tctttccttc gatatttctg gaacaaaccc cagcaaccga
     3661 tacgttgggt tggcctaaaa aactacatcg atgtttttac cgactcctta ttctggcaat
     3721 cgctgaacaa tacgattatt ttcatggtgg tttcaacctg tctaacccta ttaattggtt
     3781 tgggaatcgc tctcctgatc agccgggatt tccctgggaa aaacattttt atctccctca
     3841 ttctccttcc gatgatgatt tctccggtcg ccgctgctct ctcctggaag ttcttatata
     3901 atggagaatg gggagcggtc aattactttg ttggcctact cggcataccc aagcaagctt
     3961 ggttagctga tcccaaatta gcccttccgg ctatcatcgt aaccgatatc tggttaaacg
     4021 cacctttcgt ttttttggtg atttatacca gcttgcaggc tctgcctcaa gaaccaatcg
     4081 aagcggctcg aatcgatgga ggaacgggct ggcaaatact ttccaaagtt attcttccgt
     4141 tactagcacc aaattttctg ctcattctgg ttatccgcct tatggatacc tttcgggtat
     4201 tcgatttcat ctatgttatg accagcggag gaccaggagg caagacagaa accattggct
     4261 ttttgagtta cgagagaact tttcgtcatt tcaacatggg tcagggctca gtcattgcta
     4321 tttttcttct cattgccgtc ctttcccttt cgtggtattt catcaaaacc cttcggctcc
     4381 agttgcaaga acaggctaaa taaaagggaa atgtgtattg ggaattaaac tcatagattg
     4441 aaaggtagct ttgattatga gaaaaccggt aacctataaa aaaattctct tctggatcct
     4501 tttttatttt gtcctcattt gcgttatcct ctttttcatc tttccgatct tttggatcgc
     4561 taccacctct cttaagcaaa gagttgatgt ctatactgaa aaaccggtcc ttatctttcg
     4621 agcaacctgg gaaaattatc agcgtgtttt tgaactccaa gactttagcc gttatttgct
     4681 gaatagtctt attgtcgccg gtagtgttac tcttataata atgtattttg gagctctggc
     4741 tgcttatgct ctcacccgct ggcggtttgc cggttataat tggatatcgt tagccatcat
     4801 ctcagctcgg atgtttccgc ctattgctct cctaccttca ttctttttat tcgcctcccg
     4861 tttgggtttg gtcgacactc gtcaggtttt ggtatttgcc cacgccacct atggattacc
     4921 ctttattgtt tttatgctgg ccggcttttt ccgccaaatt cctagagatt tagatgacgc
     4981 cgctttaatt gatggttgca gccgctggca gctctttaac cgaattatgc ttcccagcgt
     5041 agcacccggt ctggcagcag ctgcgattct ctcttttgta tggtcctgga atgaatttct
     5101 tttcgcctta gttctcaccc gagcttccac caaaacctta ccggtggtga tttcagaatt
     5161 cattggattt gttcaagtcg actggcaagg tttaactgca atggctacca ttttaatgat
     5221 tccatctatt atcatcactt ttgccacaca aagatacctg gttcgaggat taaccgccgg
     5281 agcggtaaaa tattaatcat ggaggaaaag ggaaatgaaa caagaactgt gggaaaccgt
     5341 caaaaaatat tatctccact ggtggaacaa cgatttcatt ggacgaatcc ccttttgggt
     5401 atcagccccc aaggacgatc ctcgatcaca aaaagctctc tttggaaaac atttatggat
     5461 ccttgaaaaa gaaaaatttg gtaccgagaa gatcattaaa aatgccaggg agatactccg
     5521 agctactttt tacggaggtt tagccttccc ctgttatttc cccaactttg gaaccgatgt
     5581 tttttccgcc tatttgggag cagaaatgga attttccgat ctttttccac cggttgccac
     5641 aggaccttcc tttattaaag aggatgttat ttcagtttcc tgggcaaaat ggggtaaccc
     5701 ggttttaacc gattactcta acctctcccg attacagatc aatgaagaca atccttattg
     5761 gcaaaaaacc cgtgaattta tccgctatgc tggccagctt tctcaaggag aatttttcat
     5821 aggagcaacc gatattcacc ccagcatgga ttctctggca gttctccgcg gcagccccca
     5881 gaatctaagt cttgatttgg tggaaaattc ccaaggagtc aaaaaagcca tggaattgct
     5941 ttggaaagtt tgggttcggg tttatgagga tacttattac aacctgatta aaaaccaaca
     6001 agaaggaacg actgcttgga taaatctctg ggcaccaggg aagttttatc cggtggaaaa
     6061 tgacctctct ctgatgattt cgaccgatat gtatcgggag ttcttcctgg aagaattggt
     6121 caaagaaatc aattatttag actactccat ctatcattta gatgggaaag acgctcttca
     6181 ccatctggat atgattttag acatccccaa actaaatgct attcaatggg tcgctggtgc
     6241 cagcgaaagt gttgcaggag tagccaagtg gattcctctc tataaaaaaa ttcaagccaa
     6301 tggaaaagcc atcatcgtct attgtaatcc cgatgaggta accctggtta tcgactcgtt
     6361 aaaacccgaa ggcctgctta tttcggttaa ttgcgaaact gaaaaagaag cccgcgaact
     6421 cctcaataat tatggttggg aagggtttta ctggtgggag taaatagtat tgcgtgcctc
     6481 tcttacaatt ttaaaatctt tggtaggagc ttgatttatc aggcccgaag cttttcagaa
     6541 tagaccctcg tgctactttc gtggtaccag atgaggataa aaatgagtgg ccctctcacc
     6601 ctgaccctct cccatcgagg agcgagaaaa aaaagttccc cctttagaaa agggggttag
     6661 ggggatttga attttttata ctgctcagtc attacgagga gcctaaccgt tctttggttg
     6721 gactatgtgg caatctcatc cacccactcc gtcattctga ggagcgaatt gtgcgacgtg
     6781 agaatc
//
LOCUS       DAOSOL010000042         6771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11324_length_6771_cov_3.524717, whole genome shotgun sequence.
ACCESSION   DAOSOL010000042 DAOSOL010000000
VERSION     DAOSOL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11324_length_6771_cov_3.524717"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PK171_01825"
     CDS             <1..506
                     /locus_tag="PK171_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT04976.1"
                     /translation="FVYVGAIPDNCGLIAKQLRDNGVTIPMYGEDGFDTPLLVETGGE
                     AAEGVIFATHVSLEDPSPIMQQFKADYQAMYGNPPENAFAALGYDAVKLMAKAIELVG
                     SDAPEKIPEGLAQIKDFQGVSGTISYEGGSQIPNKSVSVIEVKDGKFVTVKQIAPEYL
                     SDPTIAK"
     gene            648..1427
                     /locus_tag="PK171_01830"
     CDS             648..1427
                     /locus_tag="PK171_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT04977.1"
                     /translation="MPDPILKVTDLWKNFGGLSAVSDYRLELPLGMVYGLIGPNGAGK
                     TTIFNLLSGVLKPNRGRIVFNQIDITDFRPDQVTRVGLTRTFQNLRLFPSLNVEMNLK
                     IANHIHLHYSFVASLSNLPGFFHSEREVQKKVDTMLDIFGLSQYRDELATNLPYGLQR
                     KLDIARTLMTDPKVVLLDEPSAGMNTGESEDLARLILQIKERFHLTLVIVEHRMPFVM
                     GLAGIIQVLDYGSVIAQGTPEEIQNNPQVIEAYLGAGDTIA"
     gene            1420..2124
                     /locus_tag="PK171_01835"
     CDS             1420..2124
                     /locus_tag="PK171_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT04978.1"
                     /translation="MLKVKNLSVKYGVIPALQDISIEVKDGQIVSLIGANGAGKTTTL
                     KTIMGILKPANGSIVYRDEDITRMSTPQRVRKEICLVPEGRGIFNRLTVRENLLLGAY
                     HRNDEQEINLDLEKSYSLFPRLKERENQIAGTLSGGEQQMLAIGRGLMSRPKLMLLDE
                     PSLGLAPLVVRELYELIQEILKQGVTILLVEQNATMAIRVSDYVYLLETGNVKLEGIP
                     KEVESLEDVRKVYLGG"
     gene            2127..3026
                     /locus_tag="PK171_01840"
     CDS             2127..3026
                     /locus_tag="PK171_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT04979.1"
                     /translation="MLSLGYVLQQTMNGVILGCIYSLLALGMTVVYGILRLINFAHGA
                     LITLGAFFVYFVSVRYGLSFIAGIFLVIGFGGLMGLVLDLIAYRKLRGGPEVALLITS
                     IGFYTFIENFLKLIVSPQPYAFKTPDYLNILYRMRFLTFRSIDLFIILSSIAIMIAFS
                     IFIKKSKMGIAMRATAENLEVANMMGIEINKVISVAFILGSAIAAFTGFLWGAKYGQI
                     SYDMGFLTGVKAFVAVVVGGVGSIPGAMLGGFVLGVAEILSVALLPTRFAEYRDGIVF
                     AILILVLLIKPSGIMGVKEEIRV"
     gene            3023..4012
                     /locus_tag="PK171_01845"
     CDS             3023..4012
                     /locus_tag="PK171_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT04980.1"
                     /translation="MNQKRRIIPEFWFIIGGCLLFLIIFILSRTLNRYLIHIIVLMGI
                     YSIATVSLNLTNGYTGLFSLGHAAFMAIGAYTSTLLAFPLHLRESYELPLLPAFLTGA
                     DGAMPFIVALIAGGVFAALSAILIGAPVLRLRGHYLSVASLGFMVIVTTFAKTLKGIT
                     RGSMGINAIPSYTNIYWTYLWLLIAIYVVWRIVHSRFGRAMMAIRDDETAAQVLGIFP
                     MKYKLLSFVVGAFFAGVAGGLFAHFTKAIRPFEFSFAMTFQIVIMLIVGGMGTMSGPL
                     VGAISLIAFRYALKPVEEHLKVYGFIELVYAALLIIIMLWKPEGIMGKRQLKR"
     gene            4029..4790
                     /locus_tag="PK171_01850"
     CDS             4029..4790
                     /locus_tag="PK171_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT04981.1"
                     /translation="MSKKVYHPEMTYTQFKEGKFNKAIFGVGSAENHGFHLPFGNDTL
                     VSHAIAMAVAQKVDGMLVLPPVNVGVSYHYDDFPFTLTLRPEVMVEVLKDMLNSTIRQ
                     GINRIIIINGHDGNIAPIEIAARSVKVANPEAFIASLDAWWVKAGELLPQDTFEVWNG
                     LGHAGEGETSMSLYLFPELCQPEEAQGVVPDLPEGLDIKWKFNEITPYGATGDPTKGT
                     AEKGEKMFNALVDYIVKSIQDLEKNQWKYGVMMKM"
     gene            4873..6177
                     /gene="asnS"
                     /locus_tag="PK171_01855"
     CDS             4873..6177
                     /gene="asnS"
                     /locus_tag="PK171_01855"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT04982.1"
                     /translation="MDIPWVSIGRISDFEGKVVELRGWLSNKRSSGKVAFLIIRDGSG
                     FIQGTAFVGDFFTKDQLDEIKRIPIESSILIQGKVRREERAPGGYELEISSFQLVFPS
                     EEYPIQKMEHSVDYLMERRHLWLRSKKQNALLRIRNVVMMSIHEFLQSEGFILLDSPI
                     LTPAACEGTSTLFELQYFDIGKAYLSQSGQLYMEAGAAAFGKVYCLAPTFRAEKSKTR
                     RHLTEFWMLEPEVAYYDWQDNMVLQEKLVSFIVNQALKKCQNELEILERDTTPLQKIQ
                     PPFPRISYTEAIEVLKKKGSPAQWGDDFGGDEETMISEDFERPVFIHHYPAHIKAFYM
                     QPDPENPTMVLNDDLIAPEGYGEIIGGSERIHDLALLEKRLEEHHLPREAFEWYLDLR
                     RYGTVPHSGFGLGVERTVAWIAGVKHIREAIPFPRQIYRIYP"
     gene            complement(6294..6665)
                     /locus_tag="PK171_01860"
     CDS             complement(6294..6665)
                     /locus_tag="PK171_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOT04983.1"
                     /translation="MKIYIPIGILVLLLLVGCTNNTPVPSPIPLAITELSSPCGVTGE
                     TIIITGTSFGDTQGSSMVTFNGVPATVNSWSNTQIEVLVPSGAITGDVVVRVNGINSN
                     GIRFIIPCYGPMQGMIVDQIK"
BASE COUNT         1900 a         1260 c         1504 g         2107 t
ORIGIN      
        1 attttgtcta tgtcggtgct atacctgata actgtggttt aattgccaag caattgcgag
       61 ataatggagt taccatccca atgtatggtg aagatggttt tgacacgcca ctgttagttg
      121 aaaccggtgg agaagctgcc gaaggtgtca tttttgctac tcacgtttca ttagaagatc
      181 ccagcccaat tatgcagcaa ttcaaggctg attatcaggc catgtacgga aacccacccg
      241 agaatgcttt tgcggcatta ggatatgacg cagtgaaatt aatggcaaaa gcaattgaat
      301 tggttggaag tgatgctccg gaaaagattc cagaaggttt agcccaaata aaagattttc
      361 agggagtatc tggaactatc agctatgaag gtggcagtca gattccaaat aagtcagttt
      421 cggtcattga agtgaaagat ggaaagtttg ttacagttaa acaaattgct ccagagtatt
      481 tgtccgatcc aaccattgca aaatagcatt tttactaaaa gcggactcat tgatatgagt
      541 ccgcttttgt tttgctctat ctttccttcg tcgtttcctt ttttaatgaa atttagattg
      601 tcttaacccg gcagtctaaa aagagagatc tatgaacgga gaaagaaatg cctgatccaa
      661 tcttgaaagt gactgatttg tggaaaaatt tcgggggcct ttccgcagtt agtgattacc
      721 gccttgaact tcccctggga atggtttacg gacttatcgg ccccaacggt gctggaaaaa
      781 caactatttt taatctttta agcggggttt taaaacccaa tcgaggtcgc attgttttta
      841 atcaaattga tataactgat tttcgtcccg accaggtaac ccgggttggc ttaactcgaa
      901 ccttccaaaa tcttcggctt tttccatcac ttaatgttga gatgaattta aaaattgcta
      961 accacatcca tttacattat tcttttgttg cttcgctttc caatttaccg ggtttttttc
     1021 actccgagag ggaagtacaa aaaaaagttg ataccatgtt ggatattttt ggtttatctc
     1081 aatataggga tgaactggca accaatttgc cttatggatt gcaaagaaaa ttagatattg
     1141 ctcgaacctt gatgacggat ccaaaggtgg tacttttaga cgaaccatcg gcaggaatga
     1201 ataccgggga gtcagaagat ttggcccggc ttattctcca aattaaggag agattccatc
     1261 tcactctggt tatcgttgag catcgaatgc cttttgtgat ggggttagcc ggtattattc
     1321 aggtattaga ttatggttcg gtgattgccc agggtactcc tgaagaaata caaaataacc
     1381 ctcaggttat tgaagcttat ttaggagcgg gtgataccat tgcttaaagt taaaaatctt
     1441 agtgtgaagt acggagtgat accagctctt caggatattt caatagaagt gaaagacggc
     1501 caaatagtat ctctcattgg ggccaatggt gctgggaaaa cgaccacatt aaaaacaatc
     1561 atgggaattc tgaaaccagc caatggaagc attgtttatc gtgatgaaga tatcacccgg
     1621 atgtcgacac cacaacgggt tcgcaaggaa atttgtttgg ttcctgaggg aaggggcatt
     1681 tttaatcgtt taaccgtaag agaaaatctt cttttaggag cttatcatcg aaatgatgaa
     1741 caagaaatta atttagattt ggaaaaatct tactccctat tcccacggct caaagaaagg
     1801 gaaaaccaga tagctggtac gctaagcggg ggagaacagc aaatgttggc aatcggtaga
     1861 ggtttaatgt cacgaccaaa actgatgctt ttagatgagc catccttagg tttggctcct
     1921 ctggtggtcc gagaactcta tgaattgatt caagagatat taaagcaagg tgtaaccatt
     1981 ctcctagtcg agcaaaatgc aacaatggcg atacgagttt ccgattatgt atatctatta
     2041 gaaacgggca atgtgaaatt agaaggaatt cccaaagaag ttgaatctct tgaagatgtg
     2101 agaaaggtct atctgggagg ttgattatgc tttcactggg ttatgtatta caacaaacga
     2161 tgaatggtgt cattttgggt tgtatttatt ccttgttagc attgggaatg acggttgtct
     2221 atggaatatt acgacttatc aactttgctc atggggcgct catcacttta ggggcttttt
     2281 ttgtttattt tgtgagtgtt cgttatggat tatcctttat tgcaggtata tttttggtaa
     2341 tcggatttgg cggtttaatg ggcttagttt tagaccttat tgcctatcga aagttgcgtg
     2401 gggggccaga agtagcttta ttgattacct cgattggttt ttatacattt attgaaaact
     2461 ttttaaaatt aatagtatct cctcaaccct atgcatttaa aacccctgac tatttaaata
     2521 ttctttatcg aatgcgattt ttaacttttc gatcaattga tctatttatt attttatctt
     2581 caatagcaat tatgattgcc ttttcgattt ttattaaaaa aagcaaaatg ggaattgcca
     2641 tgagggctac tgcagaaaat ttggaagtgg ccaacatgat gggtatcgaa attaataagg
     2701 tcatatcggt tgcctttatt ttaggctcag caatcgctgc ctttaccggc tttttatggg
     2761 gtgctaaata tggacaaatt tcttatgata tggggttttt aaccggtgtg aaagcttttg
     2821 tagctgtcgt cgttggaggg gttggaagta tacctggagc tatgttggga ggttttgtat
     2881 taggtgtagc cgagattctc tcggtagcat tgctccctac tagattcgcc gagtatcgag
     2941 atggaatagt ttttgccatt cttatattag ttttactcat caagccttca ggaattatgg
     3001 gagtgaagga ggagattcga gtatgaacca gaagagaaga atcattccag aattttggtt
     3061 cataattgga ggctgtctcc tcttccttat catcttcatt ctttcccgga ctctcaaccg
     3121 atatctgatt catattattg tccttatggg catatattca atcgctaccg tgagtcttaa
     3181 cttgaccaat ggatataccg gtcttttttc tcttgggcat gctgcattta tggcgatcgg
     3241 tgcctatact tctaccttgc tggcttttcc gctccatctg agggagtcat acgaacttcc
     3301 acttttaccc gctttcttga ctggtgccga tggagccatg ccctttatcg ttgctttaat
     3361 cgccggagga gtgtttgctg cactatcagc aattctaatt ggagccccgg ttctccgatt
     3421 aagagggcac tatctctcag ttgcttcatt gggtttcatg gtcatagtga ccacctttgc
     3481 caaaactctt aaggggatca cccggggatc aatgggaatc aatgccatac cttcttatac
     3541 caatatctat tggacctatt tatggctcct catcgcaata tatgtggttt ggagaatagt
     3601 tcattcccgt tttgggcgtg ctatgatggc aatccgagat gatgaaaccg ccgctcaggt
     3661 tttggggatt tttccgatga agtataaact gttgtctttt gttgttggtg ctttttttgc
     3721 cggcgtggcc ggtggtttat ttgctcattt taccaaagcc atacgtcctt ttgagttttc
     3781 atttgctatg accttccaaa tagtgatcat gcttattgtg ggagggatgg gtactatgag
     3841 tgggccttta gttggggcaa tctctttaat tgcttttcga tatgcgctaa aacctgttga
     3901 ggaacatctg aaggtctatg gttttatcga attggtttat gctgctctcc tcattatcat
     3961 tatgctctgg aaacccgagg gtattatggg gaaaagacaa ttgaaaagat aagaaagaag
     4021 ggatggatat gtcaaaaaaa gtatatcatc cagaaatgac ctatactcaa tttaaagaag
     4081 gaaagtttaa caaagcaatt tttggggtgg gatcggcaga aaatcacgga ttccaccttc
     4141 cttttggcaa tgataccctg gtatcccatg cgatagctat ggcagttgcc caaaaagttg
     4201 atggcatgtt agttcttccc ccagtgaatg ttggggtcag ctatcattat gatgattttc
     4261 cctttacatt aaccctcaga cctgaggtta tggttgaggt tttgaaagac atgctcaatt
     4321 ctactattcg ccagggaatc aatcggatta ttatcattaa tggtcatgat ggaaatattg
     4381 ctccgattga aattgctgca cgttcagtga aagtagctaa tcccgaagct tttatcgctt
     4441 ctttagatgc ctggtgggtt aaggctggag aattattacc tcaagatact tttgaggtct
     4501 ggaatggact gggtcacgcc ggggaaggag agacatcaat gtctctgtat cttttcccag
     4561 aattatgcca accggaagaa gcacagggag tggtccctga tcttccagag ggattagaca
     4621 taaaatggaa atttaatgaa ataacacctt atggagcaac tggtgatccg accaaaggaa
     4681 ccgcagaaaa aggggaaaag atgtttaatg ctttggtgga ttatattgtg aaatcgattc
     4741 aagatttaga aaagaatcag tggaaatatg gcgtcatgat gaaaatgtag agcaataccc
     4801 tatcgaacct tttcataaaa ggatttgctt gagaatcact ttgagttctt aattacattc
     4861 tggaggaaaa agatggatat accctgggtg tcaataggac ggatttctga tttcgaaggg
     4921 aaagtcgttg aactgcgtgg atggctttcg aataaacgat caagcgggaa ggtcgccttt
     4981 ctgattatcc gcgatggatc gggctttatt caaggaacgg catttgttgg agattttttt
     5041 accaaggatc aattagatga gattaaaaga ataccgattg aatcatccat cctaatacaa
     5101 ggaaaagtgc gtcgagaaga acgtgctcct ggggggtatg agttggaaat cagctctttt
     5161 caattggttt ttccttccga agaatatcct attcaaaaaa tggaacactc ggtagactat
     5221 ctaatggaac gtcgacacct ctggctccgc tccaaaaaac aaaatgcctt actgcgaatt
     5281 cggaacgtgg ttatgatgag tatccatgag tttttacaaa gtgaaggatt tatcttgctc
     5341 gattccccaa ttttaactcc agcagcctgt gaaggtactt cgactttatt tgaactccag
     5401 tactttgata tcgggaaggc ttatttatct caaagtggtc aactctacat ggaggctgga
     5461 gcagctgctt ttgggaaagt atactgttta gccccaacct ttcgagctga aaagtcaaaa
     5521 acccgacgcc atcttaccga attctggatg ttggagccgg aagttgctta ctatgattgg
     5581 caggataaca tggttcttca agaaaaatta gtgagtttta tcgtcaatca agcgttaaaa
     5641 aaatgccaaa atgaattaga gattcttgaa cgagatacca ctcctctcca gaaaattcaa
     5701 cctccttttc cccggatttc ttataccgaa gccatcgagg ttcttaaaaa gaaggggtct
     5761 ccagctcaat ggggtgatga cttcggagga gatgaagaaa ctatgatatc cgaagacttc
     5821 gaacgcccgg tttttattca tcattatcca gcacatatca aagcttttta tatgcaaccg
     5881 gatccggaaa atccgaccat ggttctaaac gatgatctta ttgccccgga aggttatggg
     5941 gagattattg gggggagtga acgtattcat gacctcgctt tgttagagaa aaggttagaa
     6001 gagcatcatc ttcccaggga ggcttttgag tggtatttgg atctccgtcg atatgggacc
     6061 gtacctcatt ccggctttgg cttaggtgtt gagcggacag tagcttggat agccggtgtc
     6121 aagcacatcc gggaagccat cccctttcct cgccagattt atcgaattta tccctaagat
     6181 aataaaacaa cccctggtta ttcattacca ggggttgttt tattatcaaa tattgatagt
     6241 tgaatgaagt aacattaaaa ggtaattgat tcaattagtg atttagattc tcttcattta
     6301 atctgatcaa ctatcatccc ttgcattggt ccataacagg gtataataaa ccgaatccca
     6361 ttgctgttaa tcccgtttac tcttaccacc acatccccag taatggctcc actcggaacc
     6421 agcacttcaa tttgggtatt ggaccaagaa tttaccgttg ccggaactcc gttaaaagta
     6481 accatacttg acccttgagt atctccaaaa ctggtcccgg taataatgat tgtttctccc
     6541 gttactccac atggagaact aagctcagta atggctaagg gtattggaga agggacagga
     6601 gtattattag tgcaaccaac caataaaaga agaaccagta ttccaatcgg tatatatatt
     6661 ttcataagca attccccttt cactgaacta ttgagttata ttatatgagc aacgtggtca
     6721 taacaaggaa tggtaaaccg aatcccgttg ctgtttattc catccactgt t
//
LOCUS       DAOSOL010000043         6732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11410_length_6732_cov_3.898158, whole genome shotgun sequence.
ACCESSION   DAOSOL010000043 DAOSOL010000000
VERSION     DAOSOL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11410_length_6732_cov_3.898158"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..817)
                     /locus_tag="PK171_01865"
     CDS             complement(11..817)
                     /locus_tag="PK171_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOT04984.1"
                     /translation="MTWERYKNFIVDMDGVIYRGKYPLPGSDDFFRFLRERKSKIAFF
                     SNNSTITKDQYVDKLKKMDIYAIKDEIISSSSITAYYVAKENPQSKVFCIGEEGIRDE
                     LKKNGIKIIDDSSREKVDLVIVGMDRHFNFDKLTKAMRHVLNGAQLYGTNPDLTYPME
                     DGLIPGCGAILASIEACTDTKAKVFGKPRPESIQFLLEMTGFSIGDTILIGDRLDTDI
                     VLAKQQNIFSILVLTGVHQGEDVKKTGIIPDMIVENLIELKKIMLMKGEA"
     gene            956..1792
                     /locus_tag="PK171_01870"
     CDS             956..1792
                     /locus_tag="PK171_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT04985.1"
                     /translation="MLEVNQGDVFGFLGNNGSGKTTTIRILFGLVRPSRGTFQVFNYL
                     CPAHLNQAKQVMGGVIDIPSFYDNLSGRENLHLLASLSNFRVSNSEIESVSSMVGIET
                     IINKPVGFYSNGQKRRLSIAQALLPRPKIIILDEPTSGLDPQGVKSIRNLILQLNQEH
                     GLTVFFSSHLLSEVQKICNRISIINKGKIIKTIDVKDFLFDSLFSIQATPVEALLSFL
                     KEKNIPYRTQEKYILVHFEGNQIPEIIQELTKRNILIYEIFRHQLSLEEAFLATIEGD
                     DQ"
     gene            1789..2604
                     /locus_tag="PK171_01875"
     CDS             1789..2604
                     /locus_tag="PK171_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT04986.1"
                     /translation="MNILGSEIQKIRANYRTYGGLIVLWGLGILIGINVWYNPSLLPL
                     KQLGFRLSGLYIPGLTLYPLAVVGPVVAVLIAGESIAGERIKGTLRMILTRPVPRVRI
                     YLGKLSLIILYSLFIVFSTLLITSLLGFFLFGGGSVVLPVELNNMSAGFFTLSAQEAI
                     LRLILAGLTVSFYILTYASIALCLSSFMSHSLASPIFTLIFTAASTVVQNLEVFKPIS
                     PFLITRHLLSWQNLMNQTIEWNSLIFSYIIVGFYFLGSLLIGIIIFNRKDETT"
     gene            complement(2627..4672)
                     /locus_tag="PK171_01880"
     CDS             complement(2627..4672)
                     /locus_tag="PK171_01880"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOT04987.1"
                     /translation="MVKDSFSQVKKRDGSIEPFTIKKIIRAIEQALLATGFYQPGKAE
                     GLAHEVIENLSINRERPIPLVEEIQDEVERVLMSHSLLQVAKAYILYREKRREIREAK
                     WKLFGIHDDLKLSFNAIQVLKERYLVKNEEGEVQETPKAMMKRVARAVAEADRLYGQD
                     VSESYEKFYHLLTSLEFLPNSPTLMNAGTPLGQLSACFVLPVEDSIEGIFNTLRDMAI
                     IHKSGGGTGFNFSQLRPKGDRVSSTGGRASGPVSFMRIFDTATDVVKQGGRRRGANMG
                     ILRFNHPDVREFIRAKNQAGFLENFNLSVAISDEEMERVHQGEKIDLIDPHLRKAVST
                     INARELFDLIVESAWKSGEPGIVFLDEINRNNPTPSLGSLEATNPCGEVPLLPYESCN
                     LGSINLSKMSADGKIDYQKLQRVVFDAVHFLDNIIDINRYPLPMVEKIVKANRKIGLG
                     VMGFADLLCKLGISYHSEQALQIAEQLITFITSEAKMASKKLAEKRGVFPNYPKSIPA
                     TKNLPLRNATLISIAPTGTISLIAGCSSGIEPYFALIYRRYVLENTELIEINPLLEEK
                     LKALKIEEKIRKSIWEKGSLKDIDGIPEHLHSLFLTALEIPPTFQVQIQAVFQKQVDN
                     AVSKTINLPTDATLEDVGQAYFLAHQLGCKGITVYRYQSRESQVLYLGSGAKECENC"
     gene            complement(4675..4803)
                     /locus_tag="PK171_01885"
     CDS             complement(4675..4803)
                     /locus_tag="PK171_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04988.1"
                     /translation="MVVWVCESCGFEKEGRCKPQKCSNCGGKGSFIKKEENQKKEE"
     gene            complement(4903..5562)
                     /locus_tag="PK171_01890"
     CDS             complement(4903..5562)
                     /locus_tag="PK171_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT04989.1"
                     /translation="MTFKLMIWDLDNTLIGSSKLLWGAFNMVFEKYAGTKLTPNEIVQ
                     LYGPPEGDVIERIVGRQRKNQALEDFYTFYRENHDLLINTFSRVFDLIGKLRQGGIKQ
                     SLFTSKGRRSADITLERLGIDKLFDFVVCGDEILRPKPNPDGVIKILSYFSLSPEEVI
                     YFGDSPLDIQSAHQAGVKGALVLWDSIHQDALGGEKPDFIFLDIPSLEKWVEQYYAWI
                     E"
     gene            complement(5552..6610)
                     /gene="hisC"
                     /locus_tag="PK171_01895"
     CDS             complement(5552..6610)
                     /gene="hisC"
                     /locus_tag="PK171_01895"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOT04990.1"
                     /translation="MSYFRKHLELIAGYQPGEQPQDGQYIKLNTNENPYPPSPLVVET
                     LMENLTKHRLSYYPSPLCDELREQASAVYGYPSDWILVGNGSDELLSIVFRAFLGKGD
                     WVLYPYPTYTLYRVLALLQEANFGEIPFEKDYSIPKKVLTGQAQLKVVCNPNSPTGTF
                     LSTMIIENLIKNSSCPVIVDEAYVDFAYDNALSLLKRFPNLIILRTLSKSFSLAGIRV
                     GFLIAQPEMIQGLLKVKDSYNVNWFSQKAAMAALRDLHYMRNNVEKIKQTRKEFSQAM
                     SKLGYEVLPSEANFVMVRKPGENLESEYLYLKKNHILVRYFTEWPDSMRISIGTDSNM
                     QLIIQILKNMTKDNKNDL"
BASE COUNT         1998 a         1415 c         1138 g         2181 t
ORIGIN      
        1 aaacaacacc ttaagcttcg cctttcatca acataatctt ttttaactca atgaggtttt
       61 caacaatcat atcaggaatg atgccggttt tctttacatc ttctccctgg tgaactccgg
      121 ttaaaaccaa aattgaaaaa atattctgtt gcttagctaa aacgatatcg gtatccaaac
      181 gatcaccaat taaaatcgta tctccaatag aaaaaccggt catttccaac agaaattgga
      241 ttgattctgg tcgtggtttt ccaaatactt tggctttggt atctgtacag gcttcaatac
      301 tggctaaaat tgctccgcaa ccaggtatta atccatcttc cattggatag gtcaaatcag
      361 gattggtacc atataactga gcaccattta aaacatgcct catcgctttg gtcagtttat
      421 caaaattaaa atgccgatcc atccccacta taaccagatc aaccttctca cgggaggaat
      481 catcaataat ttttatacca tttttcttta attcatcgcg gattccttcc tctccaatac
      541 aaaacacttt ggattgagga ttttcttttg caacgtagta ggcagttatc gagctggagc
      601 taataatctc atccttgatt gcataaatat ccattttttt tagtttatcg acatattgat
      661 ctttggttat ggtggagtta ttcgaaaaaa aggcaatttt tgattttcgt tcccgaagaa
      721 agcgaaaaaa atcatcactc ccgggaagag gatatttccc ccgataaata actccatcca
      781 tgtcaacaat aaaattttta tatctctccc acgtcaaata cacccttctt ctcaacccct
      841 tttttgaata taatactctg tgataagaaa ggagtgaact ccattcggat atccagctct
      901 taagtttaat catattacca aaagctatgg tgaaaataca gtcctcaagg atattattct
      961 cgaggtcaat caaggtgatg tctttggttt cctgggaaat aacggctctg ggaaaaccac
     1021 cactatacgt attttattcg gtttggtacg cccttctcgg ggtacttttc aagtttttaa
     1081 ctatctctgt ccagcccatt taaatcaggc taaacaagtg atgggtggag ttatcgatat
     1141 cccatcattt tatgataatt taagtggaag ggaaaattta cacttacttg cttcgctttc
     1201 caactttcgg gtaagcaatt cagaaattga atcggtttca tccatggttg ggatagaaac
     1261 cattatcaac aaaccagtag gattctattc caatggtcag aagagaagat tatcaattgc
     1321 tcaagcatta cttccccgac caaagatcat cattttagac gaaccgactt ctggactcga
     1381 tcctcagggt gtgaaaagta ttcgaaattt gatactccaa ctcaaccaag aacatggact
     1441 caccgtattc ttttcttccc atctcctcag tgaagttcag aaaatttgta atcgtatttc
     1501 cataattaat aaggggaaaa taataaaaac cattgatgta aaagactttt tatttgactc
     1561 tttattcagc attcaagcaa ctccggtcga ggctttgctc agttttttaa aagaaaagaa
     1621 tattccctac cgaactcagg aaaaatatat tctggtccat tttgaaggaa atcaaatacc
     1681 tgaaataata caagaactca caaaaagaaa cattttaatt tatgaaatat tccgtcacca
     1741 actcagtttg gaagaagcct ttctggcaac tatcgaagga gatgatcaat gaatattctt
     1801 ggttctgaaa tacaaaaaat cagggcaaat tatcgaactt atgggggtct aatcgtctta
     1861 tggggattgg gaatactgat tggaattaac gtttggtaca atccttctct tctccctcta
     1921 aagcaacttg gttttcgatt aagtggtctc tacatccccg gtttaactct ctatccttta
     1981 gcagtcgtag gaccagtagt tgcggtttta attgctggtg aaagtattgc cggtgaacgg
     2041 attaaaggga ctctccgtat gattctaacc cgaccagtcc cccgcgttcg catttattta
     2101 ggaaaattaa gtttaattat cctctactca ctttttattg ttttttccac tttattgatt
     2161 accagtctct tgggcttctt tctttttgga ggtggttcag tggttctacc ggttgaacta
     2221 aataacatga gcgctggctt ttttacctta agtgcccaag aagcaatatt aaggcttatc
     2281 ttagctggcc tgaccgtttc attctatatt ctcacctatg cttccattgc tctatgtctt
     2341 tcttccttta tgagccattc tttggccagc ccaattttca ccttaatttt taccgcagcc
     2401 tcaacagttg ttcaaaacct tgaagtcttt aaacctattt ctcctttcct gataactcgt
     2461 catttacttt cctggcaaaa cttaatgaat cagactatcg aatggaattc tttaattttt
     2521 tcctatatta ttgttggatt ttactttctg ggaagccttt tgattggaat tatcattttt
     2581 aaccgaaagg atgaaactac ttaattaaat ttacatccat tcataattaa cagttttcac
     2641 attcttttgc tccggagcct aaatataaaa cttgactttc tcgactttga taacgataga
     2701 ctgttattcc tttgcaaccg agttgatgag ccaaaaaata ggcctgacca acatcttcga
     2761 gggtggcatc ggttggaagg ttgatggttt tagacactgc attatcaact tgtttttgaa
     2821 atactgcctg gatttggact tgaaaagttg gagggatttc aagagcagta agaaaaagcg
     2881 aatgaagatg ttcaggtatt ccatcaatat cttttagaga acctttttcc cagattgatt
     2941 ttcgaatttt ctcttcaatt tttaaggcct ttagcttttc ttccagtaag ggattaattt
     3001 caatcaactc ggtattttct aaaacataac gtcgataaat aagggcaaaa tacggttcta
     3061 taccactgga acatccggca atcagactaa ttgttccggt aggagcaatc gaaatcaaag
     3121 ttgcatttcg aagaggtaaa ttcttggtag cagggatgct ctttgggtaa ttgggaaaaa
     3181 cacctctttt ttctgctaat tttttcgaag ccatcttcgc ttcagaagta ataaaagtta
     3241 tgagctgctc agcgatttga agagcttgtt ctgaatggta agaaatgcca agcttgcata
     3301 gcaaatcggc aaatcccata acccccaaac cgatttttcg attggctttt actatttttt
     3361 caaccattgg taagggataa cggttgatat caatgatatt atctaaaaaa tggacggcat
     3421 caaaaacaac tctttgcagt ttttgataat caatctttcc atcagcactc attttggaaa
     3481 gatttatgga acctaaatta caagactcat aggggagtaa tggaacttct ccgcaaggat
     3541 tggttgcttc gagtgacccc aaagaaggag tggggttatt tcgattgatt tcatctaaaa
     3601 aaactatacc tggttctccc gatttccaag cactttcaac aatcagatca aatagctctc
     3661 gagcgttaat cgttgatacc gcttttctta aatgcgggtc aatcaagtct attttttcac
     3721 cctggtgtac cctttccatt tcttcatcag aaatggctac cgatagatta aagttttcaa
     3781 gaaaacctgc ttgattcttg gctctaataa actcgcggac atccggatgg ttgaagcgaa
     3841 gaattcccat attggctcca cgtctccgac ccccttgctt cacaacatcg gtagctgtat
     3901 caaatatccg cataaacgaa accggtcccg aagctctgcc gccagttgat gaaacccgat
     3961 ctcccttggg gcgaagctga gaaaaattaa aaccagtacc tcctcctgat ttatgaatga
     4021 tagccatatc tctcagggta ttaaaaatgc cttcaattga atcttcaacc ggaagaacaa
     4081 aacaagctga aagctgtccc agcggggttc ctgcattcat caaggttggt gaattcggta
     4141 aaaactctag ggaggtgagc agatggtaaa atttttcgta actttcacta acatcctgtc
     4201 catataaacg atcggcttca gccaccgctc tcgctactcg cttcatcata gccttgggag
     4261 tttcctgaac ttcgccttct tcgtttttaa ccagataccg ttcttttaga acttggatag
     4321 cattgaagct cagctttaaa tcatcgtgaa tcccaaataa tttccacttg gcttctctaa
     4381 tttctcttcg tttttcacga taaaggatat aggcttttgc aacctgtagt aaagagtggc
     4441 tcattaaaac tctttccact tcgtcctgaa tttcttcaac cagtggtatt ggtctttccc
     4501 gattgatact caagttttct attacttcat gagctaaccc ttcagccttc cccggttgat
     4561 aaaaaccagt agcaagaagc gcttgctcta tggctcggat tatttttttt atggtgaaag
     4621 gttctattga tccatctctt ttttttacct gtgaaaaaga atctttaacc aactttattc
     4681 ctccttcttt tggttttcct ccttttttat aaaagaacct ttcccaccac agttggaaca
     4741 tttttgcggc ttacaacgac cttctttttc aaatccacaa gactcacaaa cccaaactac
     4801 cactttcatc tccacctttc tgattgtcaa tcatttttaa atatattttt ctatcaataa
     4861 cacttcgatt tttaccacca acctatcgat gatgcctttc actcattcaa tccaagcata
     4921 atattgctca acccacttct ccaatgaagg tatatctaaa aaaataaaat ccggcttttc
     4981 accacccaaa gcatcttggt gtatcgaatc ccataaaacc agggctccct ttaccccagc
     5041 ttgatgagcc gattgaatat ctaaaggtga atctccaaaa tagatcactt cttctggaga
     5101 aagggaaaaa tatgaaagta tctttatcac tccatcagga ttgggttttg gacgaagaat
     5161 ctcatcacca cagacgacga agtcaaacaa tttatcgata cctaaccttt ctaaagtgat
     5221 atccgcactt cttctcccct tcgaagtaaa tagtgattgt ttgattcctc cttgccgaag
     5281 cttgcctatc aaatcaaaaa cccgagaaaa agtattgatt aatagatcat gattctctcg
     5341 atagaaagta taaaaatctt ctaaagcttg attcttcctt tgccttccaa cgattctttc
     5401 tattacgtca ccttctggag gcccataaag ttgaacaatt tcattcgggg ttagctttgt
     5461 accagcatat ttttcaaaaa ccatattaaa agcgccccaa aggagctttg aactgccaat
     5521 aagagtattg tccaaatccc aaatcattaa tttaaaggtc atttttatta tccttggtca
     5581 tatttttcag aatctggatt ataagctgca tattcgaatc agtcccaatg gatattcgca
     5641 tagaatctgg ccattcagta aaatatctga cgagtatatg gtttttttta agataaagat
     5701 attctgattc taagttttct cctggtttgc gaaccataac aaaattcgct tccgaaggga
     5761 gtacttcgta tccgagtttg ctcatagctt gggaaaattc cttccgggtt tgtttaattt
     5821 tctccacatt attcctcata taatgtaggt ctcgtagagc tgccatagca gctttttgag
     5881 aaaaccagtt tacattataa gaatctttta ctttcagtaa accttgaatc atttcaggtt
     5941 gggcaatcaa aaatcctacc cgtataccag ctaatgaaaa agatttagac aaagttcgta
     6001 aaataatcaa attagggaat ctttttaaca gtgaaagagc attatcatag gcaaaatcaa
     6061 cataggcttc atcaactata actggacagg atgagttttt gattaaattt tcgataatca
     6121 tcgttgataa aaaagttccg gttggagagt tgggattaca gacaaccttt aattgagctt
     6181 gaccggttaa aactttctta ggaattgagt aatctttttc aaaaggaatt tctccaaaat
     6241 tggcttcttg cagcaaggca agaactcgat aaagggtata ggttggatat ggatataaaa
     6301 cccaatctcc ttttcccaga aatgcccgaa aaactatgct taaaagttca tctgacccgt
     6361 ttcccaccaa gatccaatcg gaaggatacc catatactgc tgaagcttgc tccctcagtt
     6421 catcacaaag tggtgatgga tagtaggata agcgatgctt ggttagattt tccatcaaag
     6481 tctcaacaac caacggagag ggtggatagg gattctcgtt ggtatttaat ttaatatatt
     6541 ggccatcttg tggttgctca ccaggctgat agccggctat taattccaaa tgttttcgaa
     6601 aatagctcat cgaggaactc ctttccccat ctgctttaat atttgtgtgc tccgaccact
     6661 gggatcatat atagtatata tctccatcaa atttcgaaaa tctccacgaa aatctagccc
     6721 atacccgacg ac
//
LOCUS       DAOSOL010000044         6730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11418_length_6730_cov_3.614682, whole genome shotgun sequence.
ACCESSION   DAOSOL010000044 DAOSOL010000000
VERSION     DAOSOL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11418_length_6730_cov_3.614682"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PK171_01900"
     CDS             complement(<1..1088)
                     /locus_tag="PK171_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOT04991.1"
                     /translation="MPVLKTLLDNEFDLFEPIHLAQNLVRISSVSRTRGESDLARYIA
                     NYLIDHQIRVEWQEVEEGRANIIAEIDGGRGEGPCLLLNGHLDTVPVGTGWTMPPLGG
                     QVIDNYLYGRGSCDMKGSLAAMMYTAKIVSLFTPNLFGKLKLVFVVDEEQDNLGIKKW
                     IEIWQKNWEPIDFAVVGEPTGLNISLGHRGVAAFRVTIKGKSCHAGIAHRGINAIYIA
                     SEILQFIKEKQNEFDQYEDEDIGKPALSVGKIQGGTSPNVVPDLCYFEVDVRTVPGLS
                     LHDIESLICDSVKKVFEIHDLPPTFEIEQSIPHLPPVKIPRDVPGIDILSRSISDIPG
                     EMPIFAPFPASCEAAFLDQVDIPTVIFGP"
     gene            complement(1128..1712)
                     /gene="pyrE"
                     /locus_tag="PK171_01905"
     CDS             complement(1128..1712)
                     /gene="pyrE"
                     /locus_tag="PK171_01905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT04992.1"
                     /translation="MREQEILQLFREAGAFMEGHFLLTSGLHSPFYIEKFKLLQRPKY
                     VELLAGELIERFSGASPDVVVGPAIGGIILAYEVARQLGIRMAFTERENGKMKFRRDF
                     VLEKNNSVLVVEDVVTTGSSAQEVIDVVEETGAKIIGVGILVDRSGGKIQFKYPFHPL
                     LQMEVMTYSPDECPLCQEHIELQKRGSRNLSANP"
     gene            complement(1709..2626)
                     /locus_tag="PK171_01910"
     CDS             complement(1709..2626)
                     /locus_tag="PK171_01910"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOT04993.1"
                     /translation="MNLQVHLGNLTLSNPVILASGTAGYGREIAPYLDLDRIGALTLK
                     GISSTPWVGNPPPRIHETYAGIMNSIGLENKGFDRFLREDLPFLENFQTRIIANIWGK
                     TIEEYSDIAKKMNGLERIDAIEVNVSCPNIEQGGESFSCNSQILCQLIQNLRQKIQKP
                     LIFKLGPNTDDLDNVLLFMEKEGVDILSITNTYPALAVDVENQSFIFSRKTAGLSGPA
                     IKPLALKLVYDVIQKTRLPIIGMGGIMKAEDALEYLLIGAQAVALGSVNLVDPSAAVQ
                     IIDGIEAYLIQKNIFDINDLIGKVREVWR"
     gene            complement(2623..3396)
                     /locus_tag="PK171_01915"
     CDS             complement(2623..3396)
                     /locus_tag="PK171_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOT04994.1"
                     /translation="MKKAKIVKKKFLGTSFVELWLEENDIARLACPGQFVMISAGSTY
                     DPFLKRPLGILSCQENYFGLLFDIVGRGTKILSQLEIGDQVDLLGPLGNGFSLNPKEA
                     LLIGGGRGLVPLYFLAQIFTSKKIPFQFFFGIRNQEEKVLIDYLSELSIPMVLSCQEQ
                     LSNYYCGTVLDAFEDWLHHHSQSKPAQKIYACGPQGMFQALSKMEDIIPNQIEVSLES
                     RMGCGYGVCLSCAVKKKGLPGYFHVCKDGPVFRLGEIEL"
     gene            complement(3393..4316)
                     /gene="pyrF"
                     /locus_tag="PK171_01920"
     CDS             complement(3393..4316)
                     /gene="pyrF"
                     /locus_tag="PK171_01920"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT04995.1"
                     /translation="MNFADRISRCIQEFGPLVVGLDPHIEYLPRFILEQEFKEKGKTW
                     EALARAVTLTNQKILDALHDAVGMIKIQMAFYEMLGIPGMLALKSTIEYAQNLGYIVI
                     LDGKRNDISSTALAYAQGYLSSVSFPGGQLESFWKVDALTINPYLGEDGLIPFIGEAD
                     KADRGIFVLCRTSNKSAPTLQDVDGPDKVYIKVAKMVETLGEKMIGESGFSSIGIVVG
                     ATYPDDLKYLRTNFPSLLFLIPGVGTQKGDIKSLANAFRDDGLGAIINVSRNVIFAYR
                     EDLNNVSGEGFEKKAREKAVYYQERFWEFIR"
     gene            complement(4352..5647)
                     /locus_tag="PK171_01925"
     CDS             complement(4352..5647)
                     /locus_tag="PK171_01925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOT04996.1"
                     /translation="MNKVLIKNGTLILPQSNRTIEADVLIDDGVIVQIEPGINDGKAE
                     RINASGYLVGPGFINLHVHFREPGEEKKEDLFSGSRAAVKGGFTSVLCMPNTRPPIDS
                     PLLISYLLSRAREVGLVNVFSAATISLGLEGKAMTDLGLLMNAGAKALSDDGKCVKDS
                     RLLYLLMTYSRYFHLPIILHEEDIDIAGEGQVNEGSMAVKMGYRSLPKVAEDSMIARD
                     LVLAHSTGATVHFTHLSTALSVKLIEWFQNQGAKVTADVTPHHLLLDDQSIIQYGSRA
                     KVKPPLRDQTDIVALKEGIQRGVIGILASDHAPHTNEDKEGDFNEAAFGISNLEITVP
                     LYVKALIDDQSISWLDFWKLLSFNPAQFLGLTKKGSLEIGMDADIAIIDPETHHEVKV
                     NEFVSKGKNCPFDGWQLKGWPVTTIVNGKILMRHSKLEA"
     gene            complement(5644..6573)
                     /locus_tag="PK171_01930"
     CDS             complement(5644..6573)
                     /locus_tag="PK171_01930"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOT04997.1"
                     /translation="MKWKHKHLLGIDQLSGEEIRFLLEKGNQYREWLEQSPKKLTRLS
                     GYYLINLFFEASTRTRVSFEMAGKLLGMEVVNFTNELSSLNKGESFRDTVRTLARMKV
                     DALVIRHRSSGIPLYISQFLPFHIINAGDGMREHPTQALLDLLTMKRDGIDFTRNTVA
                     IIGDILHSRVARSLSIGLKKLGSQVIFSGPPTLIPKGIEVLEGQVVYPVEEAIRQADV
                     VYLLRIQKERQEAGFFPSAKEYASFFGLKEKPKGPDGRWKKIMHPGPVNRGLEIDSSL
                     VEKENSLIEEQVSCGLAIRMAVLETLILGGANG"
BASE COUNT         1980 a         1542 c         1217 g         1991 t
ORIGIN      
        1 ggtccaaaaa tgacggttgg tatatcaacc tgatccaaaa aagctgcttc acaggaagct
       61 ggaaagggag caaaaattgg catttcccca ggaatatctg aaattgaacg gctgagtatg
      121 tcaatccctg gaacatcacg aggaatctta actggcggaa gatgggggat cgactgttca
      181 atttcaaaag ttggtgggag gtcatggatt tcaaacactt ttttaactga atcacaaata
      241 agagattcta tatcatgaag ggacaaaccg ggaacggttc tgacatcgac ttcaaaataa
      301 cacagatccg gaacgacgtt tggtgatgtc ccaccttgga tttttccaac actgagagcc
      361 ggcttgccaa tatcctcatc ttcatattgg tcgaactcat tttgcttttc tttaataaat
      421 tgcagaatct ctgaggctat ataaatagcg tttattcctc gatgagcgat tccagcatgg
      481 caagattttc ctttgatggt tactcgaaaa gcggcaactc cccgatgtcc taagctaata
      541 ttcaagccgg ttggttcacc aaccactgca aaatcaattg gttcccaatt tttttgccag
      601 atctctatcc atttctttat tccaagatta tcttgttctt catcaacaac aaaaaccaat
      661 tttaattttc caaacagatt gggagtaaaa agggaaacaa tttttgctgt atacatcatg
      721 gcagcaagcg atcccttcat atcacaggac cctcggccat aaaggtaatt atcaatcact
      781 tggccaccca agggtggcat agtccaacca gttccaaccg gaaccgtatc taaatgtccg
      841 tttagcaaaa ggcaaggacc ttctcccctc cctccatcaa tttcagcaat tatattggct
      901 cgaccttctt cgacttcttg ccattccact cgaatttggt gatcgataag ataattggcg
      961 atataacgag ctagatccga ttcccctcgg gttcgagaaa cactggaaat acgaactaaa
     1021 ttttgggcta agtgaatggg ttcaaaaaga tcaaactcgt tatccaatag tgtttttaaa
     1081 acgggcatta ctggtttttc tccttcaaaa caagtcttta ccttaaatca gggatttgct
     1141 gataaatttc ggcttcctcg tttttggagt tcaatatgtt cttgacagag aggacactca
     1201 tcgggcgaat aggtcataac ttccatttgc aatagtgggt gaaaggggta tttgaattga
     1261 attttacctc cgcttcgatc aaccaatatc cccactccaa taatctttgc tccggtttct
     1321 tctactacat caatcacttc ttgagcagaa gaaccggtgg taaccacatc ttctacaacg
     1381 agaactgaat tatttttttc aagaacaaaa tcccgccgaa acttcatctt tccattttct
     1441 ctttcagtaa aagccattcg tatccctaat tgtcgtgcga cttcataagc aagaataatg
     1501 cctccaatag ctggacccac aacaacatct ggtgatgctc ctgaaaagcg ttcgatgagt
     1561 tccccagcca ataattcaac gtattttggc cgttgcagta atttgaattt ttctatataa
     1621 aaggggctat gtaaaccgga ggtgagaaga aaatgacctt ccataaaagc tccagcctcg
     1681 cgaaataact gaaggatttc ttgctctctc atcgccaaac ctccctcact ttcccaatta
     1741 aatcatttat gtcaaagata tttttttgaa tcaggtaagc ttcgatacca tctatgattt
     1801 gaacggcagc cgaaggatcg acgagattta ccgatcccaa ggctactgct tgagccccaa
     1861 taaggagata ttcaagggca tcttccgcct tcattatacc acccattccg atgatcggaa
     1921 gtcgggtttt ttgtataaca tcatagacca atttaagcgc caacggttta atcgccggac
     1981 cggataatcc tgctgttttt ctagaaaaaa taaagctctg attttcaaca tcaacagcta
     2041 atgctggata ggtattggta atactcaata tatctacacc ttctttttcc ataaaaagaa
     2101 ggacattatc caggtcatcg gtattgggtc ctaatttaaa aatgaggggt ttttgaattt
     2161 tctgccgcaa gttctgaatg agttggcata agatttgact attacaagaa aagctttctc
     2221 caccctgctc gatattggga caagaaacat tcacctcaat agcatcaatt cgttctaatc
     2281 cattcatctt tttggcaata tcagaatatt cttcaatggt ttttccccaa atattagcaa
     2341 taatcctggt ttgaaaattt tctaagaaag gaaggtcttc ccgaagaaag cggtcgaatc
     2401 ccttgttttc taaaccaatt gagttcatga ttcctgcata agtttcatga attcgaggtg
     2461 gtggattccc cacccaagga gttgagctta ttcctttgag agttaaagct ccaatacgat
     2521 ccaggtctaa ataaggagca atttctcgcc catacccagc tgttcctgaa gccaaaatta
     2581 ctggattgga tagggttaaa tttcctaaat ggacttggag attcatagtt caatttctcc
     2641 taatcgaaat accggaccat ctttacaaac atgaaaatac ccaggaagcc ctttcttttt
     2701 cactgcacaa cttagacaaa caccgtatcc gcatcccata cgtgattcga gagaaacttc
     2761 aatttgatta gggataatgt cttccatttt gcttaaagcc tgaaacatcc cctgtggtcc
     2821 acaagcataa attttttgag caggttttga ttgagaatga tgatggagcc aatcctcaaa
     2881 agcatccaaa actgttccac aataataatt agataattgc tcttgacaac ttaataccat
     2941 agggatagag agctcggaga gataatcaat aaggactttt tcttcctgat tacgaatgcc
     3001 gaagaaaaac tgaaagggaa tttttttgga agtaaaaatt tgggctaaaa aataaagagg
     3061 aactaatccc cgacctccac cgattaaaag agcttctttg gggtttagcg aaaaaccatt
     3121 tcccagaggc ccaagcagat cgacctggtc tccaatctca agttgagata aaattttggt
     3181 ccctcggcca acaatatcaa aaagcaaacc aaagtagttt tcttgacaag ataaaattcc
     3241 gagcggtctt tttaaaaaag gatcataggt tgaaccagca gaaatcatta cgaattgtcc
     3301 cggacaagca aggcgagcaa tatcgttttc ttctaaccac aattctacaa atgatgttcc
     3361 aaggaatttt ttcttgacaa ttttggcttt tttcatcgaa taaattccca aaatctttct
     3421 tgataatata ctgctttttc tcgagctttt ttctcaaatc cctccccgga tacattgttc
     3481 aaatcttccc gataagcaaa aatcacattg cgagacacgt taataattgc acctaaacca
     3541 tcgtcacgaa aagcattggc caacgatttt atatcacctt tttgagttcc tactccagga
     3601 attaaaaaca gcaacgaagg aaaattggtt cgaagatatt tcaggtcatc aggataagtt
     3661 gctccaacga cgatcccaat agaactgaat cctgattcac cgatcatttt ttctcccaag
     3721 gtttcaacca tcttagccac cttaatatag actttatccg gaccatcaac gtcctgtagg
     3781 gtgggtgcgc ttttattcga ggttcgacac aacacgaaaa tacccctgtc tgctttatct
     3841 gcctctccta taaaaggaat caagccatct tctcccaaat agggatttat cgttaaagca
     3901 tctaccttcc aaaaagattc taattggcca ccgggaaagg aaaccgatga gagatatccc
     3961 tgtgcatagg cgagtgcagt actactgata tcattgcgct ttccatctaa aatgacgata
     4021 tatcccaaat tttgggcata ttcgatggtt gacttgagcg ccaacatccc tggaattccc
     4081 aacatctcat aaaaagccat ctgaattttt atcataccca ctgcgtcatg aagtgcgtcc
     4141 aatatttttt gattagttag agtgactgca cgagccaagg cttcccaggt ttttcctttt
     4201 tctttgaatt cttgctcgag gataaaccgg ggaagatatt cgatatgggg atctaatcca
     4261 accaccaagg gaccaaactc ttgaatacag cggctaattc gatcagcaaa attcataccc
     4321 ttcggttctc ctttactatc tttcttagac tctaagcttc caatttcgaa tgtcgcatca
     4381 atattttacc atttactatc gttgtcaccg gccatccttt taattgccat ccatcaaaag
     4441 gacaattttt tccttttgaa acaaattcat tcacctttac ctcatggtgg gtttctgggt
     4501 caatgatggc tatatccgca tccataccaa tttctaaact tccttttttg gttaatccta
     4561 aaaattgagc cggattgaag ctgagtagtt tccaaaaatc aagccagctt attgattgat
     4621 catcaatcaa cgccttcaca taaagtggga ctgtaatttc caagttggaa ataccaaatg
     4681 cagcttcatt aaaatcgccc tctttatcct cattggtatg tggagcatga tcggatgcta
     4741 aaatcccaat aactcctcgc tgaattcctt ctttcagggc aacgatatca gtttgatccc
     4801 ggagaggtgg cttcactttg gctcgacttc catattggat aatactttga tcatcaagta
     4861 gcagatgatg gggagtaaca tcggcggtca ctttggctcc ttggttttga aaccattcaa
     4921 ttagtttgac actcaatgct gttgacaaat gggtaaagtg aaccgtggct ccagtagaat
     4981 gggctaggac taaatctctg gctatcattg aatcttccgc aacttttggt aaagaccggt
     5041 atcccatttt cactgccata cttccctcgt tgacttgacc ttctccagct atgtcaatat
     5101 cttcttcatg gagaataata ggtaaatgaa aatatcgtga gtaagtcata agcagataaa
     5161 gcaaccggct gtctttaacg cattttccat catcgcttaa tgcttttgcc ccggcattca
     5221 tcagcagccc cagatcggtc atggcctttc cttctaatcc caaactgatt gtggcagccg
     5281 aaaaaacatt gaccaaacca acctcacgag ctcgactgag aaggtaggaa atcaataagg
     5341 gtgaatcaat gggaggacga gtattgggca tacataaaac ggaggtaaac ccacctttta
     5401 ccgctgctcg agaaccagaa aaaagatctt cttttttttc ttccccaggt tcgcgaaaat
     5461 gaacgtgaag attaataaaa cccggaccta ccaaatatcc gctagcattg atcctttcag
     5521 cttttccatc attgatcccc ggttcaatct gaacgatcac tccatcatcg attaatacat
     5581 cggcttctat ggttcgattg ctttggggaa gaattaaagt gccatttttt atcaatacct
     5641 tattcatccg tttgcccccc ccaagataag agtttctaaa accgccatac ggatggctaa
     5701 tccacaactt acctgctctt caatcaggga attctccttt tcaaccaaag atgaatcgat
     5761 ttccaaccct cgattgaccg gcccaggatg cataattttt ttccaacggc catcaggacc
     5821 cttcggtttt tctttcagac caaagaaact ggcatactcc ttggcactgg ggaaaaatcc
     5881 cgcttcctga cgttcttttt ggatgcgaag taaataaacc acgtcggctt gtcggatcgc
     5941 ttcttctacc ggataaacaa cttggccttc caatacttca ataccctttg ggatgagggt
     6001 tggcggaccg gaaaaaatga cctgacttcc taattttttg agtcctatac tcaacgaacg
     6061 ggcaactcgg ctatggagaa tgtctccaat aatggctacg gtatttcggg taaaatctat
     6121 tccatctctt ttcatggtga gcaaatcaag aagggcttga gtaggatgtt ccctcatgcc
     6181 atcaccagca ttgattatat gaaaaggaag aaactgggat atataaaggg gaatcccact
     6241 ggatcgatgg cgaataacca aagcatccac tttcatccgc gctagagttc gcactgtatc
     6301 acggaagctc tcacccttat ttaaactgct caattcattg gtaaagttta ccacctccat
     6361 ccccaaaagt tttccagcca tttcgaaaga tacccgagtt cgagtgctcg cttcaaaaaa
     6421 taggttaata agataatagc ctgataatcg cgttaacttt tttggagatt gttctaacca
     6481 ctcgcggtat tgatttcctt tctcaagaag aaatctaatt tcttcaccgc tcaattgatc
     6541 aatacccaat aagtgtttat gtttccattt cactccccat tgaccccctt ctttcctttt
     6601 aatatcaaga tgagtgacga gcgagcaatg aggagtgaaa aagcgtcgtc atccggatcg
     6661 gtatctttcc gtataaggac ttcatctttt cgtctttttg ctcctctcac tctaaccact
     6721 gcttccctcc
//
LOCUS       DAOSOL010000045         6692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11497_length_6692_cov_3.391743, whole genome shotgun sequence.
ACCESSION   DAOSOL010000045 DAOSOL010000000
VERSION     DAOSOL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11497_length_6692_cov_3.391743"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PK171_01935"
     CDS             complement(<1..187)
                     /locus_tag="PK171_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT04998.1"
                     /translation="MESNTIRQEIKKLREIIRQHDYRYYIINQPTVTDDEYDALMRKL
                     QELEKIHRIVAKQIPGAP"
     gene            complement(206..1495)
                     /locus_tag="PK171_01940"
     CDS             complement(206..1495)
                     /locus_tag="PK171_01940"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOT04999.1"
                     /translation="MPLAIISGTHWGDEGKGKMVDFISQKANMVIRAQGGSNAGHTVV
                     VEAKKYVFHLIPSGILHPGIKCILGDGMVIDPADLLQEIQFLRDNGWEESLSRLFISE
                     KAHLVMPYHRLFDQLQESFRQSSRIGTTGRGIGPAYEDKVARWGIRAVDLLNLDTFKK
                     KLQLVLNYKNAILEKVFQKPPLSFKEVFEQFEKYSDQLKPYIVNTLPIIYDALASHQN
                     LVVEGAQGTMLDLDHGTYPYVTSSHPVAAGSLLGASLGPLSNIQSVGICKAYTSRVGE
                     GPFPTECLDQTGETLREQGGEYGSTTGRPRRCGWLDGVVLKYATRINALTSLVITKLD
                     VLGGFSKIFFCTHYRIGNQVTSELPTDPFDWEIAQPVYQELEGWESDISKIQFYNDLP
                     RATHDYIELIEDFIEIPVAILSVGQDRSSTIVRKEIF"
     gene            complement(1547..3082)
                     /gene="guaA"
                     /locus_tag="PK171_01945"
     CDS             complement(1547..3082)
                     /gene="guaA"
                     /locus_tag="PK171_01945"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT05000.1"
                     /translation="MDKIVILDFGSQYTQLIARRIREYQVYCEIWPYHLKECDLNSSD
                     IKGIILSGSPYSVADPRAPQLEEMILKSNKPLLGICYGLHLLVKTLGGKVVQSQTREY
                     GKALLTILEKRGIFEGFGNEMVCWMSHGDSVKEIPPQFQCIARTETCDYAAIVDDQRR
                     IWGIQFHPEVSHTPLGKEILSNFIFNVCGCSSDWTAESIIKQQIQWLRNTAQKEKVIC
                     ALSGGVDSVTAAVLTYRAIGDQLSCIFVNNGLMRKGEPEHVLRNMEALLGKGRVIAVD
                     AKDRFLNELQGVVDPEQKRKIIGRVFIEVFEEEARNLGDATCLLQGTTYPDVIESISV
                     CGPSVKIKSHHNVGGLPELMHLRVIEPLRFLFKDEVRQLALELGIPDEIVWRQPFPGP
                     GLAVRIIGEVTENRLEILRDMDYIIYQELKNSPIFKKLWQSFGVLVPVRSVGIMGDER
                     TYKYVGVLRAVMSEDGMTADWAKISYETLDIIARRVVNKVAGIGRMVFDITSKPPATI
                     EWE"
     gene            complement(3082..4248)
                     /locus_tag="PK171_01950"
     CDS             complement(3082..4248)
                     /locus_tag="PK171_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HOT05001.1"
                     /translation="MHEVSLGKYRMARMTYGFDEISLVPGLRTIDPLDIDLSISIGNR
                     HLNLPILGAAMDGVIDPIMAVTMGKLGAMGVLNLEGIFCRYQDPYPLISTFIQKSREE
                     ITSYLQKTYQEPIQKSLIKERIEQIKSQGVLTAASVTPSRAQTLGIAAIEAGLDILVI
                     QSTVTTREFLSSRSAAFDLKSFCQDLPIPVIVGNCATYEVALELMKAGASGILVGIGP
                     GAACTTRAVLGIGIPQITATIDAAAARYDYWKETGRYVTVITDGGMRVGGDVAKAIAS
                     GADAVMLGSPLASAQEAPGQGHHWGMATPDPNLPRGTLVNVGIKGTLKEILLGPSHVT
                     DGTMNFIGALRGSMGSLGAQNIKEMQQVPIDISPSIWTEGKFLQKIQGVGMGRS"
     gene            complement(4623..6206)
                     /gene="groL"
                     /locus_tag="PK171_01955"
     CDS             complement(4623..6206)
                     /gene="groL"
                     /locus_tag="PK171_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT05002.1"
                     /translation="MPKKLIFDEEARRTLEKGANIITDTVKLTLGPKGRNVILEKKFG
                     APTITNDGVTIAREIEVKDPFENIGVTLVREVATKTNDIAGDGTTTAMVLAQKMIHNG
                     LKNVTAGYNPVFLKNGMDKILVQIVNKINQNSIPIDDQKSIAQVASISAKDQSIGQII
                     AESIEKVGRDGVITVEESKSTDTTLEVVEGMQFDRGYLSPYMVTDQERMVCILENPYI
                     LITDSKISSIQTLLPILQQVVQTGKPLLIIAEDLEGEALATLVVNRLRGALNVAAVKA
                     PAFGDRRKEILHDLAILTGGQVISQEMGMKLEKVTIDLLGKAGSVKINKDNTIIVDGQ
                     GSPDDIKAREKEIRVQIEKTDSSYDREKLQERLAKLIGGVAIIKVGALSETELKEKKH
                     RVEDALSATRSAIEEGIIPGGGSLLLHIRQEVSVDNLSNDEQVGALIVLNALEEPLKR
                     IAENAGYQGNIIASKVRGLDKKIGFNAMTGEFVDMVKSGIVDPAKVTRAALQNAVSIA
                     SLALTTQTIIAEHKEETPE"
BASE COUNT         1874 a         1467 c         1342 g         2009 t
ORIGIN      
        1 gcggtgcgcc gggaatctgc ttggcgacga tgcgatggat cttttccaat tcctgtagtt
       61 ttctcattaa ggcatcatat tcatcatcag taaccgttgg ttgattaata atataatacc
      121 gatagtcgtg ttggcgtatt atttctctta gttttttaat ttcttgacgg atggtgttgc
      181 tttccatact tggaaaacca cctttttaaa agatttcttt ccggacaatg gtggaacttc
      241 gatcttgacc aaccgagaga attgccactg ggatttctat aaaatcttct ataagttcaa
      301 tgtaatcatg ggtagcccgg ggaaggtcat tataaaactg gatttttgag atgtcgctct
      361 cccacccttc caattcttga taaaccggtt gagcgatttc ccagtcgaaa ggatcagttg
      421 gaagttccga agttacttgg ttaccaatac ggtaatgagt acaaaagaaa attttcgaaa
      481 agccacctaa gacgtcaagt ttggttatta ccagagaggt cagggcatta atccgagttg
      541 catacttgag caccactcca tccaaccaac cacagcgcct tggccgacca gtggtggatc
      601 cgtactctcc tccttgttca cgcagagttt caccggtttg atcgagacat tcggtgggga
      661 aaggtccttc tcctactcga gaagtatagg ctttgcagat tccaaccgac tggatattag
      721 aaaggggacc taagctggct cctagcaatg agccggcagc tactgggtgg gaggaggtaa
      781 cgtaaggata ggttccatga tctaaatcca acattgtgcc ctgggctcct tcaacaacca
      841 gattctgatg ggaagcaaga gcatcgtaaa taataggtaa agtattgacg atatacggtt
      901 ttaattgatc agaatatttt tcaaattgtt caaaaacttc tttgaaagaa agtggaggtt
      961 tctgaaaaac cttttccaga atggcatttt tataatttaa aaccagttgg agcttttttt
     1021 taaaggtatc caggtttaaa aggtcgacag ctcgaattcc ccatcgggca actttatcct
     1081 cataggcagg accgattcct cgaccggtag tcccgattcg ggaactttga cgaaaagatt
     1141 cctggagctg atcgaataaa cgatgataag gcatgaccag gtgggctttt tcactaatga
     1201 acagtcttga cagtgattct tcccatccat tatcacgcag gaattgaatt tcttgcagaa
     1261 gatcggcggg gtcaatgacc ataccatctc ctaaaatgca ttttatccca ggatgaagaa
     1321 tgcctgatgg tataagatga aagacgtatt ttttagcttc aacgacgaca gtgtgtccgg
     1381 cattgctccc tccttgagct cgaatgacca tattggcttt ttggctgata aaatctacca
     1441 tttttccttt tccctcgtca ccccaatggg tgccggagat gattgcgaga ggcatgaata
     1501 ttcctcctct ttatttttcc tatattattt atatgttatt gaatttctat tcccattcaa
     1561 tagttgctgg tggcttagaa gtaatatcaa aaaccatccg cccaatacca gcaactttat
     1621 tcactactcg gcgggcgata atgtctaagg tttcatagga gatttttgcc caatcggcag
     1681 tcattccatc ttcgctcatg acggcacgaa gaacaccaac atacttataa gttctttcat
     1741 cccccatgat tcctacactt cgaactggaa ccaacactcc gaaagattgc catagttttt
     1801 tgaaaattgg ggaatttttc aactcttgat agatgatgta atccatatcc cgtaaaattt
     1861 ccagtcggtt ttcggtcact tcaccaataa ttctgactgc aagacccgga cccggaaacg
     1921 gctgtcgcca aacaatctca tcaggaatgc ctaattctaa tgctaattgt cgaacttcat
     1981 ccttaaaaag gaatcgcaaa ggttcgatta cccgaaggtg catcagttca ggaagcccgc
     2041 caacattatg gtggcttttg attttcaccg atggcccgca aaccgagata ctttctataa
     2101 catcaggata agtagtgccc tggagtaagc aagtggcatc acctaaattt cgggcttctt
     2161 cttcaaaaac ttcgataaac acccgcccga tgatttttct tttttgttct gggtcgacga
     2221 caccctggag ttcatttaaa aaacggtctt tggcatcgac ggcaataacc cgtcccttcc
     2281 ctaataaggc ttccatattt cggagaacgt gttctggctc accctttctc atcaaaccgt
     2341 tattcacaaa gatacagctc agctggtcac cgatcgctcg ataagtcaaa actgctgcag
     2401 tcactgaatc aactccacca cttaaagcac agattacttt ttccttttga gcagtattac
     2461 ggagccattg tatttgttgt ttaatgattg attctgcagt ccaatccgag gaacaaccac
     2521 agacgttaaa aatgaagttg gataaaatct cttttcctaa gggtgtatga gacacttcag
     2581 ggtgaaactg aattccccaa attcttctct gatcgtcaac aatggcagca taatcacagg
     2641 tttcggttcg agcaatacat tgaaattgtg gtgggatttc tttaacgcta tcaccgtggc
     2701 tcatccagca aaccatttca tttccaaaac cttcaaaaat acctcttttt tccaaaattg
     2761 tcaggagagc ttttccatat tcccgagttt gagattggac caccttgcca cctaaggttt
     2821 tcactaaaag atgaagaccg taacatatcc ccaatagtgg tttattggat ttgagaatca
     2881 tctcttccag ctgaggagca cgaggatcag caacgctata gggacttcct gagagaatga
     2941 tccctttgat atcggaacta ttcaaatcgc attctttcaa gtgataaggc cagatctcac
     3001 aataaacctg atactctctg attcttcggg caataagctg ggtatattgg gaaccaaaat
     3061 ctaaaataac gattttgtcc attatgacct ccccatgccg actccctgaa ttttctgaag
     3121 aaattttcct tcagtccaaa tgctcggaga tatatcgata gggacttgct gcatttcttt
     3181 gatgttttga gcacctagag aacccattga gcctctcaag gcaccaataa aattcatcgt
     3241 tccgtcggtg acatgactag gtccaagtaa aatttctttt aaggttcctt taatgccaac
     3301 attaaccaac gtgccgcggg gaagattggg gtcaggggtt gccattcccc agtgatgacc
     3361 ttggccagga gcttcctgcg ccgaggctaa aggagaacca agcatgacgg cgtcagcacc
     3421 gctggcaatt gctttggcaa cgtcaccacc aactctcatt cctccatcgg taatgactgt
     3481 aacataccga ccggtttctt tccaatagtc atagcgggca gcagcagcat caatcgtggc
     3541 cgtaatttga ggaataccta ttcccaatac agcacgggta gtacaggcag cacctggccc
     3601 gatacccacc agaattcctg aagcaccagc tttcattaat tcaagagcta cttcataggt
     3661 tgcacaattc ccgacgatca ccggtatggg aaggtcttgg caaaagcttt ttaaatcgaa
     3721 agcagcactg cgagaagata aaaattcccg agtggtgact gtggactgga tgaccaagat
     3781 atcaagtcct gcttcgatgg cagcaatacc taaggtttga gctcgagaag gagtaactga
     3841 tgcggcggta agaacaccct gggatttaat ttgctcgatt cgttctttta tcagtgattt
     3901 ttgaattggt tcttgataag ttttttgcaa gtaactagtg atttcttctc gtgatttttg
     3961 aataaacgtg gaaatcaagg ggtagggatc ttgataacga cagaaaatac cctccagatt
     4021 gagaactccc atggcaccca attttcccat agttacagcc ataatcggat caattactcc
     4081 gtccatggct gctccaagta tggggaggtt gagatgccta ttcccaattg agatgctcag
     4141 gtcaatatct agcgggtcaa tggttcgaag tccaggaacc aaggaaattt catcaaaccc
     4201 gtaagtcatt cgagccattc ggtattttcc taatgacact tcatgcatgg gtttcacctt
     4261 cctccgttca tctcagcagt attaaaaacc aagtatagat tcacaaatct attacaattg
     4321 aataaatacg tttaacgctt ttctttaccc ctctttgaga taagatatca gctactgagt
     4381 aggtataaat aagtaggata caactcctct ccaatttgaa aggggggaaa caccaggggg
     4441 caccccaact caaaagaaca ttttgaagtt gtttgataat ggaaagagta ttcaaatatc
     4501 cccctccttg aaggggtatc ctttcgttat tatcaaaata acacacttga attaattata
     4561 aaagaactat ccgagttttc cagtgaccct tgcagttgga ttacccggat agtttacagt
     4621 ttttactctg gtgtttcttc tttgtgttcg gcaatgatgg tttgagtggt caaggctaaa
     4681 gaggcaatac tcactgcatt ttggagagct gctcgggtaa ccttggcggg atcaactatt
     4741 cccgatttga ccatgtcaac aaattcaccg gtcatagcat tgaaacctat ttttttatcc
     4801 agacctcgaa ccttactggc aataatattc ccttgatatc cagcattttc tgcgattctt
     4861 tttagaggtt cttccaaggc gttcaaaacg attagagcgc caacttgctc atcattagac
     4921 aggttatcga cggagacttc ttgtctaata tgcagaagca atgatccccc tccagggatg
     4981 attccttcct cgatagccga acgagttgct gagagagcgt cttcaactcg atgtttcttt
     5041 tctttgagtt cggtttcgga tagagcccct accttgatga tcgcaactcc tccaattaac
     5101 tttgccaatc gttcttgaag tttttcacgg tcataactgg aatcggtctt ttcaatttga
     5161 actcggattt ctttttctcg agcttttata tcatcgggac ttccttggcc gtcaacaata
     5221 atagtgttgt ctttgttgat ttttaccgat ccggcctttc ctaaaagatc tatggtgact
     5281 ttctccaatt tcattcccat ttcctgggaa ataacctgtc caccagtaag aatagccagg
     5341 tcatgaagga tttcctttcg tcgatctcca aaagctggcg cttttactgc tgcaacgttg
     5401 agtgcaccac gaagccgatt gaccactaaa gtcgctaagg cttcgccttc taaatcttcg
     5461 gcaatgatca atagaggttt tccggtttgg acgacctgct ggagtatggg gagcagcgtt
     5521 tgaatggagc taattttaga atcagtaatc aaaatatagg gattttccag aatacaaacc
     5581 attctctctt gatcggttac catataagga gataggtagc ctcgatcaaa ctgcattcct
     5641 tcaaccacct caagggtagt atcggttgac ttggattctt ctacagtaat aactccatct
     5701 ctacctactt tttctatgga ttcagcaatg atttgaccaa ttgactggtc ttttgcggaa
     5761 atggaggcaa cctgagcgat agacttttga tcatcaattg ggatgctgtt ttggttaatc
     5821 ttattgacga tttggacgag aattttatcc ataccatttt taagaaaaac tggattataa
     5881 ccagcagtga catttttcaa tccattgtgg atcatttttt gagcaaggac catggcggtg
     5941 gtagttccgt caccagctat atcattggtt ttagtagcaa cttctcggac caaagttacc
     6001 ccgatatttt caaaagggtc tttgacctca atctctcgag caatagtaac cccatcattg
     6061 gtaatcgttg gtgccccgaa ttttttttct aagatgacgt ttcgaccttt agggcctaag
     6121 gtcagcttta ccgtgtcggt aataatatta gccccttttt ctaaagtacg gcgggcttct
     6181 tcatcgaaaa tgagtttttt tggcattaat gacaccttcc cttctatctt atcgtataga
     6241 ttatttccat tttaaggaaa aaatcagact attctcgggg gaaaagtctg gcaatgcatc
     6301 gaaatctaaa gcggagtgaa ttagactggc tttagtttct tagaaatcaa caacaaaaat
     6361 atattataac gttaaggaag aaaaaaacct ataggttcat ttaaaaattt attataatat
     6421 attaatcaaa cacacctgaa aattcaatca attcctactc gctacagaat aaagcagtgt
     6481 cagtgatcaa aaaataacta ttaattgaga aatattaact attaagcttt atacttctcc
     6541 caatcgcaac tggggatcag gttctaaatc gagggggctg gttttcccta aggaaaaatg
     6601 aaaagagcca ctacttccta tcatggctgc gttatcagta catagagttg gcgatgggag
     6661 ataaactttt atattatgct ggaatccttt tt
//
LOCUS       DAOSOL010000046         6674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11545_length_6674_cov_3.671401, whole genome shotgun sequence.
ACCESSION   DAOSOL010000046 DAOSOL010000000
VERSION     DAOSOL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11545_length_6674_cov_3.671401"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PK171_01960"
     CDS             <1..638
                     /locus_tag="PK171_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05003.1"
                     /translation="AYPKIPPFIVTLASLYVYKSLAMIISVDPRFGPNPMPVSNFPES
                     FYFIGQHRIGGLPFQVLFIFIPVVLILMFLMNRTVLGRHLYGVGSNETAAIFSGINVK
                     KIRVIVYTLSGLLSALAGWVITSRIASARPDIGTGYELQAITIAVLGGVSIKGGEGTA
                     GGVVLSIAIITMLYNGMQLAGIHQIWQLGSLGLVLVGSVLLNQILYNRRKF"
     gene            641..1510
                     /locus_tag="PK171_01965"
     CDS             641..1510
                     /locus_tag="PK171_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05004.1"
                     /translation="MKRGFLKGIILLVILLFFSGPSFSTEEGPAIWLFAAQPERYFSA
                     FLEIHLKRVFSTSQLQFINLYSIQPGELEKKINPKLCLARIIILMDFSKELTDCFFRE
                     CENIEFREKPFLITWNCDLTNLPDYFHSIPITINWELVLEELQTILSGYQSPAFIVHN
                     NESYIQDFLLYLHNHSQYHLIVDDNKPCQSPPPQQVYLTSCEDIMKEYLSFSHHIICF
                     ESSTLALAEIQAGHTLASIDFKPSQIATQISDLFSNKANKFNLTLTPLLIFPESLSEA
                     DAYEVLGRCFMCK"
     gene            complement(1766..1842)
                     /locus_tag="PK171_01970"
     tRNA            complement(1766..1842)
                     /locus_tag="PK171_01970"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1806..1808),aa:Met,seq:cat)
     gene            complement(1856..1944)
                     /locus_tag="PK171_01975"
     tRNA            complement(1856..1944)
                     /locus_tag="PK171_01975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:Leu,seq:taa)
     gene            2151..2226
                     /locus_tag="PK171_01980"
     tRNA            2151..2226
                     /locus_tag="PK171_01980"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2184..2186,aa:Lys,seq:ctt)
     gene            2948..3379
                     /locus_tag="PK171_01985"
     CDS             2948..3379
                     /locus_tag="PK171_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT05005.1"
                     /translation="MKELKGIVSLSEASLLALHSMVLLAQHDSGPLTIKQMAEKTASS
                     EAHLSKVMQRLAKINLVDSIRGPNGGFYLKNPIELVTLFDIYQAIEGPLEEGSCFNKN
                     GVCPFKNCIFGGLIDKMRHEFRQYLSSKTLKDLVEKSQSDR"
     gene            3496..5142
                     /gene="hcp"
                     /locus_tag="PK171_01990"
                     /gene_synonym="priS"
     CDS             3496..5142
                     /gene="hcp"
                     /locus_tag="PK171_01990"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOT05006.1"
                     /translation="MFCYQCEQTAKGTGCTAFGVCGKDPETAALQDLLVYAILGIGQY
                     AHRVRQYGVKNHDADVFVAEALFSTITNVDFDPERLKSLLHKAYNIKEKVKQQYLDAC
                     RQSGKTPEDLSGPAIWKPADSLDQLIRQGESVTLENNIKEYGEDIAGLMHLILYGLKG
                     MAAYVDHAQILGQEDDNVYAFFHETLNFLTKGHFTLDELLKTALKCGEVNIRIMELLD
                     KANTGTYGHPVPTAVPITAKKGKAIVVSGHDLKDLAELLKQTEGKGINIYTHGEMLPT
                     HAYPELKKYPHFVGNYGGAWQDQQKEFDAFPGAILMTTNCIQKPKESYKSRIFTSGLV
                     AWPGVTHITDRNFAPVIEAALAAPGFPEDEPEKTIIVGFGHNAVMGVADQVINAVKSG
                     AIRHFFLIGGCDGAKTGRNYYTQLAQMVPQDSVILTLACGKYRFNKLEFGDIGGIPRL
                     LDVGQCNDAYSAIQIAVALANAFNTDVNGLPLSFILSWYEQKAVCILLSLLHLGIKNM
                     RLGPSLPAFVTPAALKVLQENFNLMTITTPEADLKAILGE"
     gene            5229..5543
                     /locus_tag="PK171_01995"
     CDS             5229..5543
                     /locus_tag="PK171_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT05007.1"
                     /translation="MKILEVDKTQPFVNNHNIDARRIYDYNHGQIIHMTLHPGESLKP
                     HTTPVDVAFYILEGSGLVEIGDESAPVNPGACIESPKDIPHRLINNTDKPFKFLVIKM
                     PK"
     gene            complement(5697..>6674)
                     /locus_tag="PK171_02000"
     CDS             complement(5697..>6674)
                     /locus_tag="PK171_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05008.1"
                     /translation="DFYDINDFFPMTINASMVAGKLYAIPYQVEAYTLYYRSDIFDKY
                     GVAEPNNLDELYEAAKKVKAGFDADGITDILPFTCRGIKGAGTANSAYISLVASYGAK
                     DFDKDGNPSMYTPEIVAMSDLWTKLIRDFGPKDWSALDWYDVKDAFIAGKAAMIIDAD
                     HWGAELDDPAIAQNIIGKWKALHVPEGPAGIKSNIWAWSIAMNAASENKKPAWLFMEW
                     ASSKPVMLEASLKYNNQTPVRNSVWNNEEIIKRTSEWGQGTCRPLVTKNLEEYAALQW
                     TPNPNTFALSELWQEQLQRIWTQEVSADDAIKEVQNRAMRLMPPKDWLQ"
BASE COUNT         1945 a         1411 c         1274 g         2044 t
ORIGIN      
        1 ttgcttatcc caagatccct ccttttatag tcactcttgc cagcctttac gtctataaat
       61 cattggctat gattatttcg gtcgatcctc gttttggccc caatccaatg ccagtttcca
      121 attttcccga gtctttctat tttatcggcc aacatcgtat cgggggtctt ccgtttcagg
      181 tgttgtttat ttttatccca gtggttttga tcctcatgtt tctcatgaat cgaaccgtac
      241 ttggaagaca tctctatggt gtagggagca atgaaacggc agcgatattt tctgggatta
      301 atgttaaaaa aattcgagta atcgtttata ctctctccgg cctcctaagc gctctagctg
      361 gttgggtaat aacctcgcgg attgccagcg cccgccctga tattggcact ggctatgaac
      421 tacaagccat caccatagct gtattaggag gtgtcagcat caagggtgga gaagggacag
      481 ccggcggggt tgtattaagt atcgctatca ttaccatgct ctataatgga atgcagttag
      541 cgggaattca ccagatctgg cagttgggtt ctcttggttt agttctggtg ggaagtgtcc
      601 tgctgaatca aattttatac aatcgtcgca aattttagct atgaagagag gttttttaaa
      661 gggtattata cttttagtta tcctcctttt tttctccggt ccttcttttt ctactgaaga
      721 gggaccggct atctggcttt ttgctgctca acccgaacgt tacttctcgg cttttttaga
      781 aattcattta aaaagagttt tttcgacttc acagttacaa tttattaatc tttattccat
      841 acaacccggc gaattggaaa aaaaaataaa cccaaaattg tgccttgccc gtattattat
      901 cctaatggat ttttccaaag aactaaccga ttgttttttt cgagagtgtg aaaatatcga
      961 atttcgagaa aaaccttttc ttataacttg gaattgcgat ttaaccaacc tccctgatta
     1021 ttttcattct ataccgatta ccatcaattg ggagctggtg ttggaagaac tccaaaccat
     1081 tctgtctggt tatcaatctc cggcttttat tgtccataac aatgaatcct atatccaaga
     1141 ctttctgctt tacttgcata atcattccca atatcacttg attgtcgatg acaataagcc
     1201 ctgtcaatca ccacctcccc agcaggttta tctcaccagc tgtgaagata ttatgaaaga
     1261 atacctgtcc ttttctcatc atattatttg ctttgaatcc tcaactttag ccttggctga
     1321 aatacaagcc ggtcatacct tagcctcaat tgacttcaaa ccttcacaga ttgctactca
     1381 aatttcggat cttttctcca ataaagccaa taaattcaat ctaactctta cccctcttct
     1441 tatcttcccc gaatctctct cagaagctga tgcttatgaa gtccttggtc gttgttttat
     1501 gtgtaaataa gtaggctcga tgaaataatt gagataaatg aatgctttaa ataagaggat
     1561 atcacggcga gaaaggtatt ccaattcaaa attattccca ttgcatatta ttttcatggc
     1621 ttgtagcttt ttaatttcgc gaagaatcaa tgattaataa ggaaaaacga gtctcctcta
     1681 taaagactca gaaaacaaaa aacccctctt cagaaaacca gactaagctg aaattctctg
     1741 attagagggg aaaatatagc aaaactggta gcgggggccg gattcgaacc gacgacctcc
     1801 gggttatgag cccgacgagc ttcctgactg ctccaccccg cgatatttat ttaactggtg
     1861 ccgaaggccg gaatcgaacc ggcacgggac ttgcgcccca acggatttta agtccgttgc
     1921 gtcttcctgt tccgccactc cggcacggcg aaaggaaacc tttctgacca cgccttactt
     1981 attttagcag gaaaattcgt agagtcaagc attttactgt attaatgcag ggtagaataa
     2041 aaaaccaaga gtagaggtgt cctttataaa ggcatggatt ccttaccaca tagaaattca
     2101 taccttgaca attattagtg tttcattgta tattttcact gcaataaggt gggccgttag
     2161 ctcaattggc agagcaactg actcttaatc agtaggttgc gggttcgatt ccctcacggc
     2221 ctaccagtta tttccctcat tttaaattcc tcttcattta ctttcctttt atatctctat
     2281 tttacctaac ttatcttttc ccaatgccat aaatcaataa ttctaaagtt ttactctgat
     2341 tgcctttcgc ctttcacaac tcactcctca ctgctcattg tatttgcagg atagaccctc
     2401 atgctgcttt tacaacacca gataacaatg aaaatatgta tatacatgtt aatcttcccc
     2461 tttagcaata gggggtaagg tggatttgaa ttatttcccg cttcgtcatt gcgaggagcg
     2521 tcctatgcga cgtggcaatc tcatttagca aaatattttt tatagaaaaa gataaaatga
     2581 tgagatcctc acggcttcat aaagcgaagc ctcaggatga cgccaatggc gtcagatgag
     2641 atcatcacac cctcaaaaag cgagggctca ggaagtccga ttaaagactt catttgatga
     2701 tattttcaga atagaaaaaa gaataaagac tattcttaat agattgccac atcgcttcgc
     2761 tttttgctaa gacaccaaaa accaccacca acagaagaaa agtcgcccgg tgacggagtt
     2821 ggtctcaatt aatataaagt tgtatttata gaaaaaactt gctgacttct tcaaatataa
     2881 aaaattatag aattttcctc ttgacatatt tttggtattt tagtatcttt atacctgaat
     2941 aaaagaggtg aaagaattga aaggaatagt tagtctctct gaagctagct tattggcttt
     3001 acacagtatg gtccttttgg ctcaacatga cagcgggcca ttaactataa aacaaatggc
     3061 agaaaaaact gcatcctcag aagcccatct atcaaaagta atgcaaaggc tagctaaaat
     3121 taatttggtt gactccattc gaggtcccaa tggtggtttt tacttaaaaa atccaattga
     3181 gttagttacg ctttttgaca tttaccaagc catcgagggt cccctggaag aaggcagttg
     3241 tttcaacaaa aatggggttt gcccttttaa aaattgtatt tttggtggac ttatcgacaa
     3301 gatgcgacac gagtttagac aatatttgtc ttcgaaaacc ttgaaagatt tagttgaaaa
     3361 atcgcaatca gatcgataga ccaagcaaag aactgatatt tcattgaata aacaactttt
     3421 aaactattca agttcttttt gtaagaacta agcgttagtt tataacaata atcattaaaa
     3481 aaggaggttc attatatgtt ctgttatcaa tgtgaacaaa ctgctaaagg aactggttgt
     3541 actgcctttg gagtatgtgg taaagatccg gaaactgctg ctctgcaaga tctattagtt
     3601 tatgctatcc tgggaatcgg tcagtatgcc caccgagtac gacaatacgg tgtaaaaaac
     3661 catgatgctg atgtctttgt tgctgaagct cttttttcca caattaccaa tgttgatttt
     3721 gatcctgaac gcttaaaatc tttacttcat aaagcctaca acatcaaaga aaaggttaaa
     3781 cagcaatacc tcgatgcttg ccgtcaatcg ggaaaaactc cagaagattt gagtggtcca
     3841 gcaatctgga aaccagctga cagtttagac caactgattc gtcaaggaga atcagtaacc
     3901 cttgaaaaca acattaaaga atatggtgaa gatatagctg gattgatgca ccttatcctt
     3961 tatggtctta aaggaatggc tgcctatgta gaccatgccc aaatccttgg ccaagaagat
     4021 gataatgttt acgccttttt ccatgaaact cttaattttc ttaccaaagg acattttacc
     4081 cttgatgaac tgttaaaaac tgccctaaaa tgcggtgagg ttaacattcg gattatggag
     4141 ctccttgata aagccaatac cggtacttat ggtcatccgg ttccaactgc agttccaata
     4201 actgccaaaa aagggaaggc aattgttgtt tctggtcatg acctaaaaga tttagctgaa
     4261 cttttaaaac aaaccgaggg caaaggaata aatatttaca ctcatggaga aatgcttcct
     4321 acccatgcct atccagaact gaaaaaatat cctcacttcg ttgggaatta tggtggagcc
     4381 tggcaggatc aacaaaaaga attcgacgca tttcctggtg ccatccttat gaccacgaat
     4441 tgcattcaaa aaccgaaaga aagctataaa agtagaattt tcacttccgg gttagtggcc
     4501 tggccgggag tgactcatat tactgatcgt aattttgcac cggttattga agctgcctta
     4561 gctgctccag gattcccaga agacgaacca gaaaaaacta tcatagtagg ttttggtcat
     4621 aatgctgtaa tgggagtcgc agatcaggta atcaatgcag tcaagtcggg tgccatacgc
     4681 catttcttcc tcattggtgg ctgtgatggc gcaaagacag gaagaaatta ctacacccaa
     4741 ttagcccaaa tggtaccaca agactcggtt attctcacct tagcttgtgg gaaatatcgt
     4801 ttcaacaaac ttgaattcgg tgatattgga ggtattcctc gtttgcttga tgttggacaa
     4861 tgtaacgatg cctattcggc gattcagata gcagttgctc ttgcaaacgc ttttaacacc
     4921 gatgtcaacg gtctacccct ctcctttatc ctctcctggt atgagcaaaa agcggtttgc
     4981 attctccttt cgcttctaca cctgggaatc aaaaacatga gacttggccc cagcttacca
     5041 gcttttgtta ccccagcagc tttaaaagtc cttcaggaga atttcaacct tatgaccata
     5101 actactcctg aagctgacct caaagccatt ctaggagagt aagctttcta taaatggggg
     5161 gagggttccc aactctccct ctaaatgctt accatcccct gttctataaa attattggga
     5221 ggattaccat gaaaatttta gaagttgata aaacccagcc ttttgttaat aaccacaata
     5281 tcgatgctcg taggatttac gattacaacc atggccagat tatccatatg actttacacc
     5341 caggtgagtc tttaaaacca cacaccacac cagttgatgt tgctttctat attcttgaag
     5401 gaagtggatt ggtggaaatt ggcgatgaaa gtgctccggt gaatccaggt gcttgcattg
     5461 aaagtccaaa agatatccct caccgtctca tcaataatac tgacaaacct tttaagtttt
     5521 tagttattaa aatgcccaaa taactcattt atccaaatat tatatcccct ataaattata
     5581 aagcagtaat tgttcatcta attgagataa ataaaattac agtttcccac aaaaaagagg
     5641 agctctgcca aggcagagct cctctttttt aatcttgttt atagttatgg cttttgctat
     5701 tgcaaccaat ccttgggagg catcaatctc attgcacgat tttgcacttc tttgatagca
     5761 tcatcggcag agacttcctg ggtccaaatt ctctggagtt gttcctgcca caattcgctc
     5821 agagcaaaag tgttaggatt aggagtccat tgtaacgctg cgtattcttc taaatttttg
     5881 gtaaccagag gacgacaagt tccctgaccc cattctgagg ttcgtttaat aatttcttca
     5941 ttgttccata ctgaattccg gactggagtc tggttgttat atttcaagga agcttctaac
     6001 ataactggtt tgctggaggc ccattccatg aataaccagg ccggcttctt gttttcagaa
     6061 gcagcgttca tagcaatcga ccacgcccaa atattggatt taattccggc tggtccttct
     6121 ggaacatgaa gagctttcca ttttccaatg atattttggg ctatagctgg gtcatcaagc
     6181 tcagctcccc agtggtcggc atcgatgatc atggctgctt ttccggcaat gaaagcgtct
     6241 tttacgtcat accagtctaa tgctgaccag tctttggggc caaaatcacg aatgagtttg
     6301 gtccagagat cggacatggc aactatttct ggagtataca tggatggatt gccgtctttg
     6361 tcaaaatctt tggcaccata ggaagcaacc aagctgatgt aggctgagtt ggcagttcca
     6421 gctcccttga taccacggca ggtgaaagga aggatatcgg tgataccatc ggcatcaaat
     6481 ccagctttaa cttttttggc agcttcatat aactcatcta aattatttgg ttctgctaca
     6541 ccatatttgt caaatatatc agatcgatag taaagagtgt aggcttcaac ctgatagggg
     6601 atagcataga gctttcctgc taccatactg gcattaatgg tcatggggaa gaagtcgtta
     6661 atatcataaa aatc
//
LOCUS       DAOSOL010000047         6576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11797_length_6576_cov_3.957675, whole genome shotgun sequence.
ACCESSION   DAOSOL010000047 DAOSOL010000000
VERSION     DAOSOL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11797_length_6576_cov_3.957675"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            554..1012
                     /locus_tag="PK171_02005"
     CDS             554..1012
                     /locus_tag="PK171_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05009.1"
                     /translation="MREDQLYRIPSGCALVGILNQDGSRMSGDTIIDDYPVIMIGGSS
                     RDFLGEYMAGGLIVVLGLGYDSNCSPVGKFIASGMNGGKLFIRGRFQPEQAGPGIGIE
                     KPIPEDFDEINLYLQEFSADLSLEIPELKCDDFVKVFPTTTRPYGRVYAY"
     gene            complement(1009..2187)
                     /locus_tag="PK171_02010"
     CDS             complement(1009..2187)
                     /locus_tag="PK171_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT05010.1"
                     /translation="MSKNYLLVPFFLALIVIGLGLSGIGSVVPEMTKQFGVTSAVMGR
                     VFLFHGLGYFISIMIAGFLGDIMQKSFLLRLGLLISALGFAGIASFQGFSPVILSFMA
                     MGIGLGFLDCMVNPILTEIFTKNPGTILNIVHAFYGLGSLSAPRLYAFLSQRQYNWQN
                     FYTLVTVITVVVFILFLLPFLPKSQGKMGFRNILKIFRYRAFWFMGAITMFYSGGVTI
                     LNGWLVSYFKEKGLSVPIGAVYLSFFWLGLMVGRFVLSWMVDRIGHLRMIQLNTIGGI
                     IFAALTISLPISAILTPTLLFLTGFMLSTIMPTTLTYAVVNYPETASTASGWVLTNNG
                     IATLLFPWFGGILASLTSFQTTLLMVPILLIIMLSFQWLLAGEIKKVQKSPVKTVPSG
                     "
     gene            complement(2214..2630)
                     /locus_tag="PK171_02015"
     CDS             complement(2214..2630)
                     /locus_tag="PK171_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT05011.1"
                     /translation="MKISTRARYGLRLLVDLAEHSGKDPVKLKDISQRQGISLNYLRQ
                     LIMPLESNKIVRSIRGNRGGYLLGKKPEEINLLDIMNLLEGPIDLVDCVHNKNLCQIS
                     DTCPTRKIWVEISEKMEQSLVQKSLKDLMENNEIHN"
     gene            2805..3878
                     /gene="thrC"
                     /locus_tag="PK171_02020"
     CDS             2805..3878
                     /gene="thrC"
                     /locus_tag="PK171_02020"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOT05012.1"
                     /translation="MYWPGVISHFADYLPVAPRTPELTLQEGNTPLIRSQHIVKQLKL
                     KSLFFKFEGMNPTGSFKDRGMVMAVAKAVEEGKKVIMCASTGNTSASAAAYARRAGVA
                     CIVIIPQGKIALGKLSQALMHGARVIAIKGNFDEALTIVRSITSHYPVALVNSINPFR
                     IEGQKTAAFEIVDWMQGVPDYLALPVGNAGNITAYWKGFKELFSRKEINVLPKMIGFQ
                     AQGSCPLVTGVPVEKPHTVATAIRIGNPASWKGAINARDESGGSIEAVTDEEILSAQR
                     MLAGEEGLFVEPASAASLAGVLKKNQAGFFQGNESVVCVLTGHGLKDPDIAIEQCRDR
                     IEELEASEEAIVDRLRLSGEKHD"
     gene            3871..4779
                     /gene="thrB"
                     /locus_tag="PK171_02025"
     CDS             3871..4779
                     /gene="thrB"
                     /locus_tag="PK171_02025"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOT05013.1"
                     /translation="MIEVSIPATTANLGCGFDVLGMALDLFYTVRVQPIKENKYIIRN
                     RGEGESCLPEDDRNLFFKTVKKITKMKNFPEQGLEVEAFNQIPVCRGLGSSAACISAA
                     VVTVNELNGRVLSIQDMVHFATQIEGHPDNVIPAMVGGLTIAMKNGEQIHYQKFPFFD
                     TIYLVACIPGYTLSTEEMRKILPANYSREDVIFNLSRIAYLMGSICRQDREGFFSALD
                     DAIHQPFRGQKIPGFFEVRDYLHQKGKGEAVISGSGSTILLMLREPITSSDEKKISEL
                     FAQKGNNNLVIKKVNWQKQGIRVKQT"
     gene            4776..6011
                     /locus_tag="PK171_02030"
     CDS             4776..6011
                     /locus_tag="PK171_02030"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT05014.1"
                     /translation="MSLIVQKYGGSSVADLDKVKSVACRIKSYVDQGHRLVVVVSAMG
                     KTTDRLMEMAREIHPSPPDRELDMLLSTGEQVSIALLSMALNALGQEAIALTGGQAGI
                     HTSNFHTKAKIITIDPQRVFRHLDENKVVIVAGFQGMSSDYDITTLGRGGSDTTACAL
                     TAALSAEYCEIFTDVNGVYTADPRIVPQARKINRISYDEMSEMANLGAKVLQIRSVDF
                     SQVYGVRVQVRSTFSQEEGTWVEKMRSMEDVKVRAITHNGNVIKVVFLEVPDRPGIAA
                     LIFKELADCSVSVDMIIQSEGREDKKDVAFVISREDVNRLKKVVDNLNRKIQFKGVKL
                     DEAVAKISIVGAGIASDPNIAYRMFHVLSEQEINIDMISTSNLRISCLIPNSSINEAV
                     QALHKEFIEDEEVSIHEEL"
     gene            5998..>6576
                     /locus_tag="PK171_02035"
     CDS             5998..>6576
                     /locus_tag="PK171_02035"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT05015.1"
                     /translation="MKSYNVAVVGVTGAVGQEMLRILEERNFPVKRLKQLASQRSAGK
                     IVTFCGEKIPVEVLSAESFQGIDIALFSAGASISKEFAPLAGQEGCIVIDNSSAFRQD
                     PNIPLIVPEVNPDAVIDYNKKNIIANPNCTTIVTVVPLKPLHDYGRIKRVVASSYQAA
                     SGAGAKAMQELEDQVKAYAFGQEIVATVFPYQI"
BASE COUNT         1915 a         1250 c         1550 g         1861 t
ORIGIN      
        1 tttcctggtt tggttgattc gatttatccc tctagtcggg tttttaatta atattgtttt
       61 tattacctac cttttagcct atatcggcat tttgttgact cgtttttatt taagcctgac
      121 tcgttattaa aaaaattgag ttattccaat tgacaggact actcactttt attagaattt
      181 ttttacaatt ttattaagac gttttagttt atcaagaaat ctaaattatt tatattttat
      241 aaagaaaaat tgtccagtgt tggacattaa gattgagaca agggcgtttt gctccgttgt
      301 tggagcaaag cgcccttttt ttatcgacaa aggaggtaat ttctggggtc aggtcttgat
      361 tcttgaattt taaccagcgg tgatattgaa gaaccgagga aatgggcagc agatcctcac
      421 ggcttccgat aacgaaacct ctggatgatg caacaccata attctttctc ccttgatggg
      481 aaaaaataaa gaaagggcga aatttttctc atcactgttc actgatttta aaaaggatcg
      541 aggtgaagaa atcatgagag aggaccaact ttaccgcata ccttccggat gtgccttggt
      601 tggcattctc aaccaagacg gatcgagaat gtcgggtgac accatcattg acgattatcc
      661 agttatcatg attggtggct cgagccgaga ttttcttggt gagtatatgg ccggtggttt
      721 aatagtggta ttaggtttgg gttacgattc aaattgttcc ccagtaggga aattcattgc
      781 ttcgggaatg aatggaggga agttgtttat tcgtgggcga ttccaaccgg aacaggctgg
      841 tccgggaatc ggcattgaaa agccgattcc cgaagatttt gatgaaatca atttgtacct
      901 tcaggaattt tctgctgacc ttagcttaga aattcctgag ttaaaatgcg atgacttcgt
      961 taaggttttt ccaaccacca ctcgccctta cggtagagtc tatgcctatt agccactggg
     1021 aacggttttg actggggatt tctggacttt tttgatttct cctgctaaga gccattgaaa
     1081 agaaagcata atgatgagca aaatcggcac catcaacaga gtggtttgga aactggtgag
     1141 tgaggctaat attcctccaa accacggaaa taagagcgta gcaatcccat tattggttaa
     1201 gacccatccg gaagcggttg aggcagtttc tgggtaatta accactgcat aagtgagagt
     1261 agtcggcata atcgttgaga gcatgaaacc ggttaagaag agaagagtag gggtgagaat
     1321 ggctgatatg ggaagactga tggttaaagc cgcaaaaatg attcctccaa tggtattaag
     1381 ctggatcata cggagatgac cgattcgatc aaccatccag gaaaggacaa aacgaccaac
     1441 cattaatccc agccaaaaaa aggataggta aaccgctcca attgggactg ataatccttt
     1501 ttctttgaaa taggacacta accatccatt caggatggtt actcctccgg agtagaacat
     1561 ggttatggct cccatgaacc aaaaggcccg atagcggaaa atttttaaaa tatttcgaaa
     1621 tcccattttc ccctgacttt tggggagaaa ggggaggaga aaaagaataa aaaccaccac
     1681 ggtgataact gttaccaacg tataaaaatt ctgccagtta tattgtcgtt gacttaaaaa
     1741 agcatagagt cgaggagcag atagagagcc aaggccgtaa aaagcatgga caatattgag
     1801 aatggtgccg ggatttttgg taaatatttc ggttaaaatt ggattcacca tgcagtccag
     1861 aaaaccaagc ccaataccca ttgccatgaa gctgaggatt actggggaaa aaccttgaaa
     1921 agaagcgatt ccggcaaatc ctaaagcgct aattaacaat cccaaccgaa gcaggaaaga
     1981 tttttgcata atgtcaccta aaaaaccggc gatcatgatg gaaatgaagt accccaatcc
     2041 atgaaacaga aatacccgtc ccatcacagc tgaggtgaca ccaaactgtt tggtcatttc
     2101 gggaacgacc gacccaattc cggagagtcc taatcctata acaatcaaag ctaaaaagaa
     2161 tgggaccagc aggtaatttt tggacaatcg atttcgaccc cctgttttaa tatttagttg
     2221 tgtatttcgt tattttccat cagatctttc aaactttttt ggacgagtga ctgctccatt
     2281 ttttccgata tttctaccca tattttacga gtaggacagg tatccgatat ttggcaaagg
     2341 tttttgttat gaacacaatc gactaaatca attgggcctt ctaacaggtt catgatgtcc
     2401 agcaggttga tttcttcagg ttttttccct aatagatatc cacctcggtt tccacggatg
     2461 cttcgaacga tcttattcga ttccaggggc atgataagct gccgaaggta attgagggat
     2521 atgccctgac gttgagagat atcttttaat ttaaccgggt ctttccctga atgttcggct
     2581 aaatcgacta ataatcttaa tccgtaacga gctctggttg aaattttcat gagatagcac
     2641 actacctttc taaggatatc aaaatagtat gatttttagt cggttctgtc aatgaaaccg
     2701 attggtaagg aaatcttggc gaaaagaaat caagatgtta caaatcaagg attttatgat
     2761 attctttatc acaattattt taatatgatc aaggaggcat gctcttgtac tggcctggtg
     2821 ttatttcgca ttttgctgac tatcttcctg tagctcctag gacaccggaa ttaactttac
     2881 aggaagggaa tactcctctt atccggtctc aacatattgt taagcagtta aaattaaaat
     2941 ctctcttttt taaatttgaa ggaatgaatc caactggttc ctttaaagac cgtggaatgg
     3001 tcatggcagt tgctaaggca gttgaagaag ggaagaaagt gatcatgtgt gcttcaactg
     3061 ggaatacttc tgcttcagcg gctgcttatg cccggcgggc gggagttgcc tgtatcgtca
     3121 taattcctca gggtaaaatt gccttaggga agctctcaca agcattgatg catggagcac
     3181 gggttattgc tatcaaggga aattttgatg aggctttgac gatcgtccgt tccataacct
     3241 cgcattatcc ggtggcattg gtgaattcca tcaatccctt tcgaattgag ggccaaaaaa
     3301 ccgccgcttt cgagatcgtt gattggatgc aaggggtgcc tgattatctg gctttaccgg
     3361 ttggcaatgc tgggaatata accgcctatt ggaagggttt taaagaactt tttagccgaa
     3421 aagaaatcaa tgtcctaccc aagatgattg ggtttcaagc tcaaggatct tgcccattgg
     3481 taactggggt accggttgaa aaacctcata ccgttgccac ggcaatacgg attgggaatc
     3541 ccgccagctg gaaaggggca atcaacgccc gcgatgagtc gggggggagc attgaagcgg
     3601 taaccgatga agaaatcctt tcagcgcaga gaatgttagc cggggaagaa ggattatttg
     3661 ttgagcctgc ttcagcggct tctttggctg gtgtattgaa aaaaaatcaa gccggatttt
     3721 tccagggaaa tgaatcggtt gtttgtgtac tcaccggtca cgggctgaaa gatcctgata
     3781 tcgccataga acaatgccgg gatcgaatcg aagaattgga agcatcggaa gaggcaatcg
     3841 ttgatcgcct tagattatct ggagaaaagc atgattgaag tcagcatacc ggcaaccacc
     3901 gccaacttag gatgtggatt tgatgtcttg ggaatggctc tggatctctt ttatactgtt
     3961 cgagttcaac ccattaaaga gaacaaatat attatacgaa atcgaggcga aggagaaagt
     4021 tgtctccccg aagacgatcg gaatctattt tttaaaaccg ttaaaaagat tacaaaaatg
     4081 aagaattttc ccgaacaagg cttggaagtt gaagcattca accagattcc agtctgccgg
     4141 gggttgggaa gtagcgcggc ttgtatttcc gcggcggtag tcactgttaa cgagttgaat
     4201 ggaagggtac tttcaattca agatatggtt catttcgcca ctcagattga agggcatcct
     4261 gataatgtca tccctgccat ggttggtggt ttaacgatag ccatgaaaaa tggtgaacaa
     4321 atacactatc aaaaattccc cttttttgat actatttatc tcgttgcttg tattccggga
     4381 tataccctct ctactgagga aatgagaaag attcttcccg ctaactattc acgagaagat
     4441 gtcatcttta atttatctcg aattgcctat ttgatgggga gcatttgtcg tcaagatcgg
     4501 gaaggctttt tttctgccct ggatgacgct attcatcagc cctttcgggg gcaaaaaata
     4561 ccgggttttt ttgaagtccg tgattattta catcagaagg ggaagggaga ggcggttatt
     4621 agtggttcgg gctcgacgat actcttgatg cttcgggaac cgatcacctc ctccgatgaa
     4681 aaaaagatca gtgaactgtt tgctcaaaaa ggtaacaata atctggttat aaaaaaagta
     4741 aattggcaga agcaaggaat tcgagtgaaa caaacatgag tctgattgta caaaaatatg
     4801 gtggatcttc agtagccgat ctggataagg taaaatcggt agcttgccgg ataaaatcct
     4861 atgtggatca aggacatcgc ttggtggtgg tggtttcagc catgggaaaa accaccgacc
     4921 ggctgatgga aatggcacga gaaatacacc cttcgccacc cgatcgagaa ttagacatgc
     4981 ttctatcaac cggggaacag gtatctattg ccctgctttc catggcgctc aatgctttag
     5041 gtcaggaagc gatagctctg accggcgggc aagccggcat tcatacctcg aatttccata
     5101 ccaaggcgaa gattataacc atcgatcccc aaagggtttt tcgtcacctt gatgaaaaca
     5161 aggtggtcat tgtcgctggc tttcagggga tgagttcgga ttatgatatc acgactttag
     5221 gtcgtggagg gagcgatact actgcttgtg ccttgacggc agctttatct gctgaatatt
     5281 gcgagatttt taccgatgta aatggggtat atactgctga cccaaggata gtgccccaag
     5341 ccaggaaaat caatcggata tcttatgatg agatgtccga gatggcgaat ttaggtgcca
     5401 aggtattaca aattcgttca gttgactttt ctcaggtcta tggtgtaaga gtccaggtgc
     5461 ggagtacttt ttctcaggaa gaagggacgt gggtggaaaa gatgcgatcg atggaagatg
     5521 ttaaagtgag ggcgattacc cataatggaa atgttatcaa ggtggttttt ctcgaagttc
     5581 ccgatcgacc gggtatcgca gcccttattt ttaaagagtt ggctgattgt tcagtttcgg
     5641 ttgatatgat tattcaaagc gaaggtcgag aggataaaaa agacgtagcc tttgtcatat
     5701 cccgagaaga tgtaaatcgc ttaaaaaaag tggtggacaa tttaaaccga aagattcaat
     5761 tcaaaggagt caaactggat gaagcggtag cgaagatttc catcgttgga gcgggtattg
     5821 ccagcgatcc caatattgcc tatcgaatgt tccacgttct ttctgagcag gagattaata
     5881 ttgatatgat cagtacctcg aatttgagaa tatcatgttt aattcccaat tcgagtatca
     5941 atgaggccgt acaggcgctt cataaagaat ttatcgaaga tgaggaggtc tctatccatg
     6001 aagagttata atgtcgcggt agtcggagta accggagcag ttggtcagga aatgttaaga
     6061 attcttgaag aaagaaattt tccggttaaa cgattaaaac agctggcatc acagcgatcg
     6121 gctggaaaga tcgttacttt ttgtggtgaa aagatcccag tcgaagttct gtcggcggaa
     6181 agctttcaag gaatagacat cgcattgttt agtgccggag catcgataag taaggagttt
     6241 gctcctctcg ctggccaaga ggggtgcatt gttattgata attcatcggc atttcgtcag
     6301 gaccccaata ttccattgat tgttccagaa gtcaatcccg atgccgttat tgattataat
     6361 aagaaaaata ttattgccaa cccaaattgt acgacaattg tcacagttgt tcctttgaaa
     6421 ccgcttcatg attatggaag gatcaaaaga gtcgtggcgt caagctatca agctgcttcc
     6481 ggtgctggtg ccaaggcaat gcaggaatta gaagaccaag tgaaagcgta tgcttttggc
     6541 caagagatag tagctacggt tttcccctac caaatt
//
LOCUS       DAOSOL010000048         6557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_11836_length_6557_cov_3.762996, whole genome shotgun sequence.
ACCESSION   DAOSOL010000048 DAOSOL010000000
VERSION     DAOSOL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11836_length_6557_cov_3.762996"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1439
                     /locus_tag="PK171_02040"
     CDS             351..1439
                     /locus_tag="PK171_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOT05016.1"
                     /translation="MSDGQTYNPQKLIKQILISVFISLGTIIAIFLILSFRGVSIDFS
                     QFQPIWLVGGFVSIIAAWLIDALRLFFTTRAWGKPISYRNSLTGVLSCYFMSAITPSA
                     TGGSPAEMLVLNRSGMSWGEAGCLTAICGILYQVTLLILLLILVLVLGVHFTLRGILL
                     HLLYSFLIFYSTLIFLLFFFLNRLDLVYRLVHWGMRFANRHFPKLKFSEDSVLKWVND
                     FFADFKNGFRILFINKPQYLILNIVGYSFYFVCFFSVAFFVLQSLGVYVPLKQVLGNQ
                     IPLYLVFGFLPTPGASGGVELSIGSVFLSSTGPEKISLFILFWRFITFFATMIVGAIT
                     FFRVLFTIGKKITQVAPSAKAAMNGEKQ"
     gene            complement(1449..2954)
                     /locus_tag="PK171_02045"
     CDS             complement(1449..2954)
                     /locus_tag="PK171_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT05017.1"
                     /translation="MRKLLLISMMIVISLLFVLSTGWVCAQNQSSAESNQQDLVELGL
                     SYFRLGMFSDAIQTLKKGLEGNTSDKTGWQSLAAAYRAQGLYNEAEETYQLVLRLDDK
                     DPWVWFEIGNLYYDMRDYQLAIDAYENSITLNNSQPEVQYNLGNVYYDLQSFEKARDA
                     YLKAIEIVPRYFDALNNLGNVYYYLNQTGEAEKAYLEAAAAQPGSPLPHYNLGNIYYR
                     ERKYDLAIEQYKEALDLDPEYVDALNNLGNALSELGNTEAAIEAYQKVIGIDPYYANA
                     YFNAGLAYRDLGDSQNAVLQYQKALEVVPDDAEIYYELGNVYYDEQDYQKAVENHSKA
                     VEVEPDYTAAYNNLGNAYEALGNYEKALEAYSKAVEIDPNYTTSLYNIANIYYNQGNY
                     YKSIDPFLKVVELDPQDYEAWNNLGNSYLEIGRNLNALDSYRKALRIKPDYDISHYNV
                     GLVSLRLDDKGTAMREYDYLLPINKKLADALMDEITGRSKELEAEPQESNN"
     gene            complement(3308..3550)
                     /locus_tag="PK171_02050"
     CDS             complement(3308..3550)
                     /locus_tag="PK171_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05018.1"
                     /translation="MRGVVWGGNWRVLSDVPKTMVYQIAECINQKSEVIPQDIIQKAP
                     SAELKSNQFDQDTLPPYPILIKSLSYGLKMASLSRR"
     gene            complement(3593..4840)
                     /locus_tag="PK171_02055"
     CDS             complement(3593..4840)
                     /locus_tag="PK171_02055"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOT05019.1"
                     /translation="MSTTNPFAIAQAQLDKCAEIIHLDPAIHAILRMPKREIHVSIPV
                     RMDDGSIRVFQGFRVQYNDAKGPTKGGIRFHSLETIDTVRALSAWMTWKCAVIDIPLG
                     GGKGGVICDPKSMSTGELERLSRGYIQAIWQFIGPDKDIPAPDVYTNPQIMAWMMDEY
                     SKCSGKNQFGVITGKPLAVGGTVGRGDATARGGMYALREAAEVCGVDLSQATVAVQGY
                     GNAGFYAAKLVREMFGSKVVALSDSQGGIYNPDGLVPDAVLEYKEKTCSVNGFPQCDS
                     ITNGEILSLDVDILIPAALENVITDQNAGSIKARIVAELANGPTTPEADDVLFENGVH
                     VIPDFLCNAGGVTVSYLEMVQNFYMYYWEEGRVYDCLNKKMTRAYHAVHLTSKKYSIN
                     MRQAAYIVAVERVVEAMKLRGWV"
     gene            complement(5109..6179)
                     /locus_tag="PK171_02060"
     CDS             complement(5109..6179)
                     /locus_tag="PK171_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT05020.1"
                     /translation="MNKTVLIPTFYALTAALLFGLSTPLNKLLLNTIEPLVLSGLIYL
                     GSGLGILIYRLIKEKSLYTQTEAKLQREEIPVLVAAVLAGGVLAPIILLYSIRITPAS
                     TASLLLSFEGIATTLLAGIWFGEYIGKRVWLAITIVTLATMLLSVQWSQGWGFTIGAF
                     GVIAACFMWGLDNNLTRNLSTRDPLLLVFVKGCGAGGFSLLLARFFNLSFPSFQMILI
                     AMAIGVVSYGLSIVFFILSLRSLGSSRTTALYSIAPFAGSLLSFLILGEQPQRVFYLS
                     FPLILVATLILLTETHSHQHLHNAYSHDHRHSHWDGHHNHIHPSFFQEKDIVHSHLHE
                     HEEMVHDHQHLPDIHHRHQHKK"
BASE COUNT         1825 a         1448 c         1257 g         2027 t
ORIGIN      
        1 gggagggtgc ctttaattcg tcatcctgag gcttcgtatt cagaaaccgt gaggaggatc
       61 tcatcttttt cttttttttc tccccatctc ttcctcgccc cctcggcgct tttcgccaat
      121 accccccttg gcgctttttg ccagccatac tctctaaatg tacaatcatc agaggttcaa
      181 ctataagaaa aactcctact cattcaaact ccatcatcac ttacaaaatt cttcaaaggg
      241 gcttttcgtt agaaacttgt tagggtacaa tcttttagaa ttctgatttc caccaccgat
      301 gaataaataa ccatcaaggt ttctgaggca gaatacagga gaatgaataa atgtcagacg
      361 gacaaactta caatcctcaa aagcttatca aacaaatcct catctcggtt ttcatcagtt
      421 taggaaccat tattgcaatt tttttaattt tatcctttcg gggagtatca attgacttct
      481 ctcaattcca accaatctgg ctggttgggg gttttgtttc aatcatcgct gcctggttaa
      541 tcgatgccct tcgtttattc ttcaccactc gtgcctgggg aaaaccgatt tcttatcgga
      601 atagcctgac cggggtatta tcctgctatt tcatgtctgc tataactccc tccgctaccg
      661 gtggaagtcc ggctgaaatg ctggtattaa atcgatccgg aatgtcctgg ggtgaagccg
      721 gctgcctgac cgctatttgc ggtatcctct atcaagtcac tcttttaatt cttctgctaa
      781 ttttggtttt ggttctcggt gtccatttca ccttaagagg catcctcctc catcttctct
      841 attcttttct tattttttat agtactttga tttttctcct cttcttcttt ttaaatcgat
      901 tggacttggt ttaccgatta gtacactggg gaatgagatt cgccaatcgg cacttcccaa
      961 aactcaaatt ttcagaagat agtgttttaa aatgggtgaa tgatttcttt gctgacttta
     1021 aaaatggatt tcgaattctc tttatcaata aacctcaata ccttatctta aacattgttg
     1081 gttatagttt ttactttgta tgtttttttt cggttgcctt tttcgttctt caatcactcg
     1141 gagtttatgt tcctttaaaa caagttttgg gcaatcaaat ccccctatat ttggttttcg
     1201 gcttcttacc aactcccgga gcatcgggag gagtcgagct ttccatcggt tcggtttttc
     1261 tttcttcaac cggtccggaa aaaattagct tattcatttt attctggcgt ttcataacct
     1321 tttttgcaac tatgatagtg ggagccatta cttttttccg agtactcttc accatcggga
     1381 aaaagataac tcaggttgct ccttcggcta aagctgccat gaatggagaa aaacagtaaa
     1441 ctcaaccttt aattattcga ttcttgaggt tctgcttcta gttcttttga ccttccggta
     1501 atttcgtcca tcagagcatc ggctaacttt ttgttgatcg gtaataagta atcgtattct
     1561 ctcatcgctg ttcctttgtc atcgagtctc aacgaaacca atcccacgtt gtaatgggaa
     1621 atatcataat ctggtttgat tcgtaaggcc ttccgataag aatcgagagc gtttaaattt
     1681 cttccaattt caagatagga attccctaaa ttattccaag cttcgtagtc ttggggatct
     1741 aactcaacca ccttcaggaa tggatcgatc gatttataat aatttccctg gttataataa
     1801 atgttggcaa tgttataaag actggtcgtg taattgggat caatttcaac tgccttgctg
     1861 tacgcttcta atgccttttc atagtttccc aatgcttcat aagcattacc caggttgtta
     1921 taagctgcag tataatctgg ctcaacttca actgccttgc tgtggttttc aacagctttt
     1981 tgataatcct gctcatcata atagacgttt cctaattcgt aatagatttc agcatcatca
     2041 ggaaccacct ccaaggcttt ttgatattga aggacagcat tctgagaatc acctaaatct
     2101 cgataggcta aacctgcgtt aaaataagca ttggcgtaat aaggatctat tccaatcact
     2161 ttttggtagg cttctatggc agcttcagta tttcccaatt ccgaaagagc atttcccagg
     2221 ttgtttaaag catcaacata ttccggatct aaatctaaag cctctttata ttgctcaatg
     2281 gctaggtcgt attttcgttc ccgataatag atattgccta aattataatg gggcaaggga
     2341 cttcctggtt gagcggcagc agcttctaaa taggcttttt cagcttctcc ggtttgattc
     2401 aggtaataat aaacattgcc taaattgttt aatgcatcga aataacgagg aactatctca
     2461 atagctttga gataagcatc ccgggctttt tcaaaactct gcagatcgta atatacattt
     2521 ccaaggttgt attgtacttc gggttggctg ttattaagag taattgaatt ttcataagca
     2581 tcaattgcca actggtaatc tctcatatca taataaagat taccgatttc aaaccaaacc
     2641 cacgggtctt tatcatccaa tcgcaaaacc aattgatagg tttcttcggc ttcattatat
     2701 aaaccctggg ctcgataggc agcagccagg ctctgccatc cggttttgtc acttgtattt
     2761 ccctctaatc ctttttttag tgtttgaata gcatccgaaa acattcccaa gcgaaaatag
     2821 gacaatccca attctacaag gtcctgttga ttgctttcag ctgaggattg gttttgggca
     2881 caaacccacc cggtcgagag aacgaataac aaagaaatga ctatcatcat tgaaatcaat
     2941 aaaagttttc tcatggttga ccctcctctt aatcatactt tgaacggttg gtattctgaa
     3001 aaatctaagc ttacctggaa ctctggttat ttcaattttc gggcatatac cacagaagca
     3061 tttcattatt ttttttcatt atatcttatt caaacccaca aatttgtaat aaaattcaag
     3121 gagggtgaaa ttatttgctg agattgattt cagtaggaat aatcagcggc aataggcatt
     3181 cgtctgccgt ttccgaaggc cttggaggta acctttaatc ccggcggagc ttgctttctt
     3241 ttatattcat tctggtcaat tctctgcacc gcccacaata ccatcctgcg atcaaatcct
     3301 caagcggtca tctcctcgat agagaagcca tcttcaatcc atagctcaac gatttgatca
     3361 aaatcggata aggtggaagt gtatcttgat cgaattgatt ggacttcagt tccgctgatg
     3421 gtgctttttg aataatatcc tggggtataa cttcgctctt ttggttgatg cattcagcaa
     3481 tctgataaac catggttttt ggaacatcag aaaggacccg ccaattcccc ccccaaacta
     3541 cccctctcat gcccaaggaa gagcacgaga gggagatatt tcaatcaaag aatcaaaccc
     3601 aacctcttag tttcatcgct tcaacaactc tttcgacagc tacgatataa gctgcctgac
     3661 gcatgttgat tgaatatttt tttgaagtca ggtgaaccgc atggtaagca cgagtcatct
     3721 tcttgtttaa gcaatcataa acccggcctt cctcccagta atacatatag aaattttgaa
     3781 ccatttccaa gtaggaaacc gtcactcctc cagcattgca gaggaaatct ggaataacat
     3841 gaactccgtt ttcaaacaaa acatcgtcag cttcaggagt tgtcggtcca ttagctagtt
     3901 cggctacaat acgagctttg atagatccgg cattctggtc ggtgattaca ttttccagtg
     3961 ctgcaggaat aagaatatct acatctaagg agaggatttc tccattggtg atggaatcac
     4021 attgaggaaa accattaacc gaacaggttt tttccttata ttccaaaact gcatcaggaa
     4081 ccaagccgtc aggattatag attccaccct gactatcgct gagggcaacg acttttgaac
     4141 cgaacatctc ccgtactagt ttagcagcgt aaaaaccagc atttccatat ccctggacgg
     4201 caactgtcgc ctggctgagg tcaactccgc atacctcggc ggcttctcgt aatgcataca
     4261 taccaccccg agccgtggca tcacctcgac ctaccgttcc tcccacagcc agcggttttc
     4321 cagtaatgac tccgaactgg ttctttccag agcatttgga atattcatcc atcatccagg
     4381 ccataatctg tggattggta tatacatctg gtgccgggat gtctttatct ggtccaataa
     4441 actgccatat cgcctgaata tagcctcggc tcaacctttc taactcgccg gttgacatac
     4501 ttttcggatc acaaatgact ccgcccttcc caccacctaa gggaatatca attaccgcac
     4561 atttccaagt catccaggca gacagagccc gaacggtatc aatggtttcc aaggagtgga
     4621 atcgaattcc acctttagtt ggtcccttag catcgttata ttgaacacga aatccctgaa
     4681 atactcttat cgaaccgtcg tccatgcgca ctggaataga aacatgaatt tcacgcttgg
     4741 gcatccggag aatagcatga atagcaggat caaggtggat aatttcggca cacttatcaa
     4801 gctgggcttg cgctattgca aaggggttag tggtagacac gttacattcc tcctcatcga
     4861 gacaagaaat tgtcctgtcg agaaattgca tctctctcca tcgagaacaa acgtccctcg
     4921 tcatcgaggt cgttgtcatt ttctcctcgt ctttgaggaa ggaggtgaac actcaccctt
     4981 aagaaatgac ttatgtaatt ttgagtaatt taccacatga tgaaaaaatt aaaaagaggg
     5041 aagagaatat tttttccatt tttttttttt ttgagataat gaaaagaatc agccggaaaa
     5101 gcggttattt actttttatg ttgatggcga tgatgaatat ccgggagatg ctgatggtcg
     5161 tgtaccattt cttcgtgttc atgaaggtgg gaatgaacga tatctttttc ttgaaaaaaa
     5221 cttggatgaa tatggttatg gtgaccgtcc caatgggaat gacgatggtc atgagaatag
     5281 gcattgtgaa ggtgctggtg agaatgagtt tcagtgagga gaattaaggt ggctaccaga
     5341 atgagcggaa aagaaaggta aaatacccgc tgaggctgct ctcccaatat gagaaaagaa
     5401 agcaatgaac cggcaaaggg ggcaatgcta taaagagccg tggtgcggga tgagcctaga
     5461 ctgcggaggg agagaataaa aaaaacaata ctcaacccat aagaaacaac accaatcgcc
     5521 attgcgatta aaatcatttg aaaactggga aaagagaggt tgaagaagcg ggctaaaaga
     5581 agagaaaatc ctccagcgcc acaacccttg acgaaaacta acaaaagagg atcccgggtg
     5641 cttaaatttc tagtgaggtt attgtccaat ccccacataa aacaggcagc tatcactcca
     5701 aaagccccaa ttgtaaatcc ccacccctga ctccattgca ccgataaaag catggttgcc
     5761 agagtcacaa tggtaattgc taaccatacc ctctttccta tatattctcc gaaccaaatt
     5821 cccgctaata aagttgttgc aataccttca aaactgagca aaagcgatgc tgtcgatgcc
     5881 ggggtaatcc gaatgctata gagaagaatg attggggcta aaactccacc cgccaaaaca
     5941 gctgcgacta acacgggaat ttcttctctt tgcagcttag cttcagtttg agtatagagg
     6001 cttttctctt ttatcaatcg ataaatgaga atgcctaatc cgcttcctaa atatattaag
     6061 ccggaaagaa ccagtggttc gatggtgttc aggagtagtt tattcaaagg agtactaagt
     6121 ccaaaaagca gtgccgccgt caaagcataa aaggtgggaa ttaaaactgt tttattcata
     6181 atgaagtctt ttctttcctt cacccttttt tatatatttt actccttagt tggttaacgg
     6241 ggaatgtcgg ggtaattatt ctgcgttttt aatcttcaac aattccttca gttcaaggtg
     6301 catagtagca tgaaaagtgc ctttgcgatt ctttgttatt ctgagacttc atattcagaa
     6361 accttggtaa tctcatccac ctaatcaatc attctaagga gtgtattatg cgatgtgaga
     6421 atctcatctg acgctatcat cgtcatcctg aggcttcgta ttcagaagcc gtgaggatct
     6481 cgttttttat tcgtattctt tatccttttc ttaggagaat caggaaaaac tgaaaacttc
     6541 ctccccgccg cgaaagc
//
LOCUS       DAOSOL010000049         6453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_12091_length_6453_cov_3.969834, whole genome shotgun sequence.
ACCESSION   DAOSOL010000049 DAOSOL010000000
VERSION     DAOSOL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12091_length_6453_cov_3.969834"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..465)
                     /locus_tag="PK171_02065"
     CDS             complement(142..465)
                     /locus_tag="PK171_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOT05021.1"
                     /translation="MIESKLGECVFTRSEIEKRVQEIAAEINQDYLGKRPLIISILRG
                     AIFFTVDLVRSLTIPFDLDFMALSNYKTGFKKVEIEKDVDEEVADRDVIIVEDIIDTG
                     LTLNY"
     gene            complement(462..1379)
                     /locus_tag="PK171_02070"
     CDS             complement(462..1379)
                     /locus_tag="PK171_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOT05022.1"
                     /translation="MEKVGIITISRETESLGDEIATQLSKQLNYECIDRERLAVLLHE
                     AHQEDLDENIDRQKFLIWAKNVKEVLLKQAKRGPIIFLGRGGQTIFQNDSRAFHLLIV
                     SSPELRLQRIMEKYRQDEVTASHILAELDRKREKFLLEALGADWKNPLIYHLVLNTAY
                     YTVNQSCQIIQEAIKNLGVHRTPKEQQLLFPSLDFGGNRVEFVHPSEEEFARILDFYR
                     IKWLYEPRTFPLEWDSEGNVVEAFSPDFYLPDFDLFIELTTQKQKLVRKKNRKVKRLK
                     DLYPKVKIKVIYSRDYTHLLRKFGVEEDI"
     gene            1738..2238
                     /locus_tag="PK171_02075"
     CDS             1738..2238
                     /locus_tag="PK171_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05023.1"
                     /translation="MNSSQSQAAAWRDVPPFNLYDLTGQEIPFSVDDQMVVVLFLSPE
                     CFSCDRELFAAQYLQKNLQFQLIPICVGCDWREVKRILESLNLDLQVYMGLNNLKATW
                     GFWEFPTAFLVDQNMKIIEKWQGKIAVESLEKEIIQNALAQAKPKRKKADGSTSPSAC
                     SDGVCY"
     gene            complement(2235..2873)
                     /locus_tag="PK171_02080"
     CDS             complement(2235..2873)
                     /locus_tag="PK171_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05024.1"
                     /translation="MKTKFIFLLTIILVFSIHSIVCSQDMFNNTLNLDSLKHQKYLIR
                     QEDKDGITEGSLTVDVVPQDEAKYQVSIDFLLGEDHFTNSFIASKDDNQNLFNNLFFS
                     NPMAMAIVMPVFAVQFMLLPVTLMGGELKEGFLWSSKEDDEELTIQVPSSEERFGLKA
                     YWVEILKDKQIAVRMLYSKEAPLPFVVEITDSDTLDEGLESLYLELQEWETN"
     gene            2996..3766
                     /gene="larB"
                     /locus_tag="PK171_02085"
     CDS             2996..3766
                     /gene="larB"
                     /locus_tag="PK171_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOT05025.1"
                     /translation="MKRVMVISVFFHTLEDLWNQKITPQEAEKQLRQLLYEDLDFVKI
                     DHHRALRKGFPEVVFCQGKTQDQILTILKKMSEQYPNLLATRAQPDHFLAVQKEIPDI
                     LYHPTAHLLVLERKPVEKKGCVTIISAGTADLPIAEEAAVTAEIAGSYINRIYDIGIA
                     GIHRLFSHLDQLWQSNVIVAVAGMEGALPGVVSGMVGRPVIAVPTSVGYGAHFQGLSA
                     LLTMLNSCSPGIATVNIDNGFGAGYLAHLINQLAEGEK"
     gene            3770..4981
                     /gene="larC"
                     /locus_tag="PK171_02090"
     CDS             3770..4981
                     /gene="larC"
                     /locus_tag="PK171_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HOT05026.1"
                     /translation="MILYCDCFSGISGDMFLGGLVDLGLPIDLLQEELTKLKISFLIQ
                     AEKVTKRAIQATQVIITDPEEHHHHTHHPDHHHHHRPAKELVRILQESDLSSSIKEKS
                     ISLLWKIAQAEGSIHGQNPEEVFLHEVGGLDTIIDITGAIIGLNYFHIKKVFASTIPT
                     GFGFIETAHGKLPIPAPATVELLKGVPIKSGHIPHELTTPTGAAIIAGITNEFGTLPH
                     MVIEKIGYGAGSHDFEIPNTLRLFLGRLSENERTEENILIETNLDDMSPQIIEYVSDK
                     LFEIGALDVFTTPIFMKKQRPAIKLSVIIQPDLFERVKNILFQETTTLGFREFQFKKV
                     FLAREDRFVETVWGKVRVKIAKHQKGFRITPEYEECKTIAQKFQIPLLDVIRSVHQTA
                     TERIELNKLAF"
     gene            complement(4996..5199)
                     /locus_tag="PK171_02095"
     CDS             complement(4996..5199)
                     /locus_tag="PK171_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05027.1"
                     /translation="MASRDYFDKRKLVLGEISLDERIDQWVWILDKRIIKRFVTLKER
                     ETLVQGLICQKCGAKCKGYCDVS"
     gene            complement(5287..6012)
                     /locus_tag="PK171_02100"
     CDS             complement(5287..6012)
                     /locus_tag="PK171_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05028.1"
                     /translation="MNAEQPLLMIDSLHVYYGGIHALKGISLEVYKNKIVSLIGANGA
                     GKSTTLRTISGLVRSKTGTIKLKDSLIGELPTHRIVEMGIAMVPEGRKIFPNLTVMEN
                     LILGCYTRKDMVQIKNDMDWIFSIFPRLKERTWQKGGTLSGGEQQMLAVGRALMSRPQ
                     LLMMDEPSLGLAPLLVKEIFHIIKKINQEGTTILLIEQNARAALEISDFAYVMETGKI
                     TISGEGKGLLYDEKVRKAYLGED"
     gene            complement(6012..>6453)
                     /locus_tag="PK171_02105"
     CDS             complement(6012..>6453)
                     /locus_tag="PK171_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05029.1"
                     /translation="KTFFSAMWETPGYIRQEKVMRAEGEQLLEAVGLLGMAGEEAKNL
                     PYGQQRRLEIARALATHPKLLLLDEPAAGMNPHETMDLMSFIQRIRKEFNLTILLIEH
                     DMKVVMGICERVAVMDFGIKIAEGTPNEIRNNPKVIEAYLGVEG"
BASE COUNT         1948 a         1345 c         1122 g         2038 t
ORIGIN      
        1 ctagcccata cccgacgaca taaatatccg ggatttcaaa gcatgaataa tgtatgggaa
       61 cctcaatgaa ccgtcgatca ggacaatcta aaaaagtaca agttacaaca ctggatggaa
      121 aacgcttttc cataacccgg gttaataatt taaggttagg ccggtatcga ttatatcttc
      181 tacgatgatg acatctcgat cggcaacttc ttcatcgaca tctttttcaa tttcaacttt
      241 cttaaatcca gttttataat tcgatagagc cataaagtcg agatcgaaag gaatggttaa
      301 cgaacgaacc aagtcaacag taaaaaaaat ggcaccgcga aggatagaaa taatcaacgg
      361 ccttttcccc aaataatcct gattaatttc agctgcgatt tcttgcactc ttttttcaat
      421 ttccgatcga gtgaaaacac attcgcccag ttttgattca atcatatatc ttcctccact
      481 ccaaacttac gtaaaagatg agtatagtct cgtgaataaa taactttaat ttttaccttt
      541 ggatataagt cttttagcct tttcactttg cggttttttt tcctgactaa tttttgtttt
      601 tgagtagtga gttcaatgaa aagatcgaaa tcgggtaggt aaaaatcagg tgaaaaagct
      661 tcgacaacat tcccttcact atcccactcc aaaggaaaag tacggggctc atagagccat
      721 ttgattcgat aaaaatcgag tatccgtgca aactcttctt cagatggatg aacaaactca
      781 actcgattcc cgccaaaatc aaggctggga aacaacaatt gctgttcttt gggggttcga
      841 tgaacaccta aattcttgat tgcttcctga ataatttgac aggattgatt taccgtataa
      901 taagctgtgt ttaaaactaa gtgatagata agaggatttt tccaatcggc gcctaaagct
      961 tcaagaagaa atttttctct tttccgatcc aattcagcca aaatatggga agcggttact
     1021 tcatcctgtc gatatttttc cataattctt tgcaaacgaa gttcgggtga agaaacgatc
     1081 aacaaatgaa aggccctgga atcgttttga aaaatggttt gtcctcctcg gccaagaaaa
     1141 attattggac ctcttttagc ctgtttaaga aggacttcct tcacgttttt tgcccaaatg
     1201 aggaatttct gtcgatcaat attctcatcc aaatcttctt gatgggcttc atgcagtagc
     1261 acggctaatc gttcacgatc aatgcattcg taattcaatt gttttgataa ttgagtagcg
     1321 atctcatcac caaggctttc ggtttctctt gagattgtaa taatacctac tttttccact
     1381 tcatatttca ccactatttt tttataccct attatatacg aattcgcctt cgatgaatgg
     1441 ttttttatac tttttcatgg tttttataaa aaattggtga ttttaaaaat ctttcaaacc
     1501 aataatttta taaaacactt ttttataatt ctctttttat cgaataccca cctggaccaa
     1561 tttacttttt tattctatcg atggtgttat gcttgtcttt tttaatcaat accagcattt
     1621 tctctcttgg agttgctaat cttcccatga aaaagtaaaa tagaaaccat gaaaaaaatg
     1681 ccatcctccg taaatctaat tttttggttt ttattagtct tgttgggaac atattttatg
     1741 aattcttccc agtcacaagc ggcggcgtgg cgcgatgttc ctcctttcaa tctttacgat
     1801 ttgaccggtc aagaaatacc tttttctgtt gatgatcaaa tggttgttgt tcttttttta
     1861 tccccggaat gtttttcttg tgatcgagaa ctatttgcag ctcaatatct tcaaaaaaac
     1921 cttcaattcc agctgattcc catctgtgtt ggatgcgatt ggcgagaagt aaaacgcatt
     1981 cttgaatctc taaacttgga tttacaggta tacatggggc ttaataattt aaaggcgacc
     2041 tggggttttt gggaattccc aaccgccttc ttagttgacc aaaacatgaa aatcattgaa
     2101 aaatggcaag gtaaaattgc cgttgaatct ttagaaaaag aaattattca aaacgctctt
     2161 gctcaagcaa aaccaaaaag aaaaaaagcg gatggctcca cttctccatc cgcttgtagt
     2221 gacggtgttt gctactaatt agtttcccat tcttgaagct ctaaatacaa tgattccagc
     2281 ccttcatcaa gagtatcgga atcagtaatt tcgacgacga aaggaagtgg ggcttccttt
     2341 gaatagagca ttcgaaccgc tatttgttta tccttcagta tctccaccca ataagctttt
     2401 aaaccaaaac gttcttcaga agaaggaact tgtatggtta attcctcatc atcttctttt
     2461 gaactccata gaaaaccttc ttttaactcg ccgcccatta aagtaactgg tagcaacata
     2521 aactgaactg caaaaactgg cataacgatt gccatagcca taggattgga gaaaaaaaga
     2581 ttattaaata aattttgatt atcatctttt gaagcgataa aagagttcgt aaaatgatcc
     2641 tcacctaaaa gaaaatcaat gctcacctgg tactttgctt catcttgcgg tacaacatca
     2701 acagtaagag atccttcggt tataccatcc ttatcttctt gtcgtattaa atatttctgg
     2761 tgttttaaac tatctaagtt caaagtatta ttaaacatgt cttgagaaca aacaatagaa
     2821 tgaatagaaa aaacaagaat aatggttaat aaaaaaatga atttagtctt cataaacatc
     2881 tcctccgttt ttcttgtatt ctaacatgca ttacctcatt tatcctactg tttttccaat
     2941 tttcttttat taagtaaggt gtccaggaat gatataatat aaaacaattt gatgcgtgaa
     3001 gagggtgatg gttatctcgg ttttttttca taccttagaa gatctctgga accaaaaaat
     3061 taccccacaa gaagccgaaa aacaattacg tcagcttctc tatgaagacc tcgactttgt
     3121 aaaaattgat catcatcgag cattaaggaa aggattccct gaggtggttt tttgtcaggg
     3181 aaaaacccaa gaccaaattt taactatcct gaagaaaatg agtgaacaat atccaaattt
     3241 attagccacc agagctcaac ccgatcattt tttggccgta cagaaagaaa taccggatat
     3301 tctatatcat ccaaccgctc accttttggt cttagaacga aagccggtcg aaaaaaaagg
     3361 gtgcgttaca ataatcagcg ccggaaccgc cgatcttcca atagccgagg aagctgccgt
     3421 aaccgccgaa attgcgggga gttatataaa ccggatttat gacattggga tcgctgggat
     3481 tcaccgtctt ttttctcatc tcgatcaact ttggcaatcc aacgttatcg tagcagtagc
     3541 cggcatggaa ggtgctttac cgggggtcgt ttccggtatg gtcggacgac cagtgattgc
     3601 agtgcccacc agtgttggct atggagctca ctttcaaggt ctctcggctt tattaaccat
     3661 gttgaactcg tgctcacctg gtattgccac ggttaatatt gataatggat tcggtgccgg
     3721 gtacctggct catcttatta atcaacttgc tgaaggagaa aaataaacca tgattcttta
     3781 ttgtgactgt ttttctggca ttagtggtga tatgttttta ggtggtttgg tagatttggg
     3841 tttacccatt gacttgcttc aagaagaact caccaaatta aaaatttctt ttcttattca
     3901 agctgaaaaa gtcaccaaga gagctattca agcaacccaa gtaataatta ccgatcctga
     3961 agaacaccat catcatactc atcacccaga ccaccatcat catcatcgac cagctaaaga
     4021 gttggttcgt atcctacaag aatccgatct ctcctcttct attaaagaaa aaagtatatc
     4081 cctgctatgg aagattgctc aggcagaagg gagtattcat gggcaaaatc ctgaagaggt
     4141 tttccttcac gaagtcggag gactagacac tataatcgat attaccggcg caataattgg
     4201 actcaattat tttcacataa aaaaggtttt tgcctcaacc attcctaccg gctttggttt
     4261 tatcgaaact gcccatggta agttacctat tccagctcct gctacagttg agttgttaaa
     4321 aggtgtaccc ataaaatcag gacatattcc acacgaactt accacaccaa ctggggcggc
     4381 tataatcgca ggcataacca acgaatttgg aactcttcct catatggtca tagaaaaaat
     4441 tggttatgga gcaggatccc atgattttga aatcccgaat acccttcggc tttttttagg
     4501 acgtttgtct gaaaatgaac gaaccgaaga gaatattcta atcgaaacca atctcgacga
     4561 tatgtcacct caaatcattg aatatgtgag tgataaactt tttgaaatcg gtgcgctgga
     4621 tgtctttact actccaattt tcatgaaaaa acaacgtccg gctattaaat tgtcggttat
     4681 catccaaccc gacttatttg agagagttaa aaatattctt tttcaagaaa cgactacttt
     4741 gggatttcga gagtttcaat ttaaaaaggt attccttgct cgcgaagacc ggttcgtaga
     4801 aacggtttgg gggaaagttc gggtaaaaat agccaaacat caaaagggtt ttaggataac
     4861 cccggaatac gaagaatgta aaaccattgc tcaaaaattc caaatcccac ttcttgacgt
     4921 gattcgttcc gttcaccaga cggcaacaga aagaattgaa ctaaacaagc tggcctttta
     4981 aaaaatattg atcaatcagg atacatcgca ataaccttta cattttgccc cacatttctg
     5041 acaaatcagc ccctgaacca gggtttcccg ttcctttaaa gtaacaaaac gcttaataat
     5101 tcttttatct agtatccata cccattgatc aatgcgttca tctaatgaaa tctcgccaag
     5161 tactaacttt cttttgtcaa aataatcacg actagccaag ttgattctct cctctatcca
     5221 gaaaattgat atctttagaa ttaagtatta aggtagtaaa gtgatatatg atataagatt
     5281 ccatccctaa tcttcgccta aatacgcctt tctgaccttt tcatcataca gtaagccttt
     5341 tccttctcca ctgatggtaa ttttaccagt ttccataaca taggcaaaat cagatatttc
     5401 caaagcagcc cgagcattct gttcaatgag caagatggtg gttccttcct gattaatttt
     5461 ttttatgata tgaaatattt ctttcactaa gagtggtgca agccctaatg acggttcatc
     5521 catcatcagt aattgaggtc gagacatgag agcccgacca actgctaaca tctgctgttc
     5581 cccaccactc agggttccac ccttttgcca ggttctttct ttcaatcgag gaaagatgga
     5641 aaaaatccaa tccatatcat ttttaatttg aaccatatct tttctggtat aacacccaag
     5701 aatgaggttc tccataacgg taaggttcgg gaaaattttt cttccttcgg ggaccattgc
     5761 aattcccatt tcgacaattc ggtgggtggg taactcgcca attaagctat cttttaattt
     5821 gatagttcct gtcttcgagc gaactaatcc agaaatggtt ctcagtgttg tgctctttcc
     5881 ggctccattc gcaccaatta aagaaacaat cttgttttta taaacctcta aggatattcc
     5941 ttttaaggca tgaatcccgc cgtaataaac atggaggcta tctatcatta ataatggttg
     6001 ctcagcgttc attatccttc cactcccaag taggcttcaa ttacttttgg attattacga
     6061 atttcatttg gtgttccctc ggcaattttg attccaaaat ccattaccgc tactctttca
     6121 cagattccca taaccacttt catatcgtgt tcgataagaa gaatggttaa attaaactcc
     6181 ttgcggattc tctggataaa actcatgagg tccatggttt catgaggatt cattcctgct
     6241 gctggttcat ctaataaaag aagtttcgga tgagtggcca aggctcgggc tatttccaac
     6301 cttctttgct gtccataggg caaatttttg gcttcctctc ccgccattcc aagcaaacca
     6361 actgcttcta acagttgttc tccttctgct ctcatgactt tttcctgacg aatatatcca
     6421 ggggtttccc acatggcact gaaaaaggtt ttc
//
LOCUS       DAOSOL010000050         6385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_12273_length_6385_cov_3.812796, whole genome shotgun sequence.
ACCESSION   DAOSOL010000050 DAOSOL010000000
VERSION     DAOSOL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12273_length_6385_cov_3.812796"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1464)
                     /locus_tag="PK171_02110"
     CDS             complement(148..1464)
                     /locus_tag="PK171_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05030.1"
                     /translation="MMHNSKILHYMLVALVVIFSVSFGAIAQDKIELIIIGHQVHLNA
                     ASSQEPSAKGDNLIEEFEALHPNVKVVYQTYPNPKTQEKLNLLGPLNRTEEDIIHVLD
                     PWFVPRVANFLEPLDKYLAEKPLEDYPNDYPEGLLSVTKIKDVPYAIPVRVVYNGIQW
                     FNQKILDEIGVAYQPTVTGPELFDIAKKGTFTRSNGEKVFGYAWRGVIGSIFEIFDRW
                     ARYFGSRLITDDLKPAVNTPGTIEGLSYMQKFYQEGIVPPDVHTYEYAEEVKLFMENR
                     STVYDGNTSYGVTFNDPTKSNIAGAAMLTKSELPPDLQAQGPHHGSAALWSMAILKGS
                     QKKDLAWEYIRFLASKKGALSMGLSGNTPVRVSTLNSDEYVQNNPAAQMEALVAPYAE
                     VYWPPFDRSNEAIDLIGNYCHDVIFRGQPVQESMDQLAKELEALLP"
     gene            complement(1655..3043)
                     /locus_tag="PK171_02115"
     CDS             complement(1655..3043)
                     /locus_tag="PK171_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT05031.1"
                     /translation="MEKVTYTQQAVVEETDVLVVGGGTAGLASALASARKGVKTTLVE
                     NSGNLGGMATIGLVGPFMTCFSSDGKDQIVKGIFDEVVRRMEKKGGAIHPANIPASSP
                     YSSFHQIGHDHVTPFDPNVFKLVAFELCQEAGVRLKLYSRFLGTQVSSAGEASSIREA
                     FIANKSGIQAIRAQRYIDCTGDGDVAAQSGVPMEKGRDLDKKMQPSSLFFRIGNVDSQ
                     KLITFVRKSYPVGKDEDGFLEIIKKGRIEMNYSVPHDRIGLYQTTRWDEWLINHTRML
                     GVDGTNVEDLTRAHMEGQRQVFEALEFMRRFIPGCDNAEVVEVADQVGVRESRRIQGE
                     YVLKVEDVYNCVPFDDCVVAAGFPVDIHDPAGSFGLFEPPRGHHYEIPYRCLLPLKVD
                     NLLVAGRCVSATHEALGAIRVMPFCFGMGEAAGTAAAISIQDGVIPKKVDTKKLQKVL
                     AEQGAFIPRISQ"
     gene            complement(3083..3931)
                     /locus_tag="PK171_02120"
     CDS             complement(3083..3931)
                     /locus_tag="PK171_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05032.1"
                     /translation="MITHKRRNLLIEKILPGILLAIVVMWAFFPIVNAIIGSLKMPLH
                     IWDFPPRFFGPFYSLTNYQELVKDWPQYFGNLVNSLIVTSGTALVVILCSIFGAYAFS
                     RFSHRLVRMSTLFTIIIRMIPPIIVVIPLFPIFNTFGLVDKHITLIMLYAAFMVSITT
                     LLMKTFVDDVPVALEEAALIDGCSRFQAFLKITIPLSAPGIVAAVIFTAIFAWNEYLF
                     AFIFTSRRAATAPLILSEFMGSFMGVEWGMLLAAAVIHLIPMLILTWMVQSYLIKGMS
                     FGAVKQ"
     gene            complement(3968..4846)
                     /locus_tag="PK171_02125"
     CDS             complement(3968..4846)
                     /locus_tag="PK171_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05033.1"
                     /translation="MRIKSRLNISMLMLVPIIALLVVVIVIPGIWAIILSFTQYTPGN
                     VPIFNDIQNYIQIIRDRFFKNALANNIIFVVVVVALEFIIGLGSALLLNRKFPLQKLW
                     VSLLIAPYCISEVVACVMWRYMLDPSYGFVNYALSFLGLGPIAWFGTVTSSFIGIIIV
                     TVWKNFPFIMIIAYSALTAMPQEVIEASKIDGANRIQTFRFVVFQLIKPALLVGLTFR
                     IIFALREFGIIWILTEGGPAGGTEILPIYLYRQTFRYYEFGRGAATTTIMLIVTILIS
                     IPLVRRIYKGMYEKIY"
     gene            complement(5017..6054)
                     /locus_tag="PK171_02130"
     CDS             complement(5017..6054)
                     /locus_tag="PK171_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT05034.1"
                     /translation="MRITIEEVAKRAGVSKATVSRVLNNKSWVAPETRQKVLKAIEQL
                     NFSPSEFAKRLGSHSERLGIVGALISESYYGSLFDNFLLFEILKGIGEIVDEHRESLL
                     LKTIKYADHQKIREKKSFSFYGNQKIVDGIIIAGIPVREQLIKDLILKGHLLVTIGRY
                     QSAPYRVLVDNYGGLYKLTDYLISLGHTKIALISGPLEFYSFHDKLLGFQDSLKNHGI
                     EVDPKLIIEEKGDPTQGGYWGMEKILTFKEQPTAVLVTDMNMMAGVFRYLQERKIRVP
                     DDISIAGYRGEDFNSLFTKSVTRIKIGERNIGRVAARLLYDILNNLVGSRMDITLSTE
                     LVIGETCKKLE"
BASE COUNT         1930 a         1429 c         1186 g         1840 t
ORIGIN      
        1 cttcatgata aagtcttttc tctaatcttt tttctaacac ctcataccta taatcattca
       61 ccaatacagt ctttaacgtt caataaaagt tatccccgcc ttttaaaaag acggggatca
      121 aagcaacctc acaaccttat tgaagtatta cggtaacagt gcctctaatt ccttggcaag
      181 ctggtccatg ctttcctgta ctggctgacc cctaaagata acatcgtggc agtaatttcc
      241 gatcaagtca atggcttcat ttgatcgatc aaaaggtggc caataaactt cagcataagg
      301 tgcgactaaa gcttccattt gagctgctgg gttattctga acgtattcgt ccgaattcaa
      361 ggtcgaaact cgaactgggg tgtttccaga aagacccatg ctcaaagcac cttttttaga
      421 agccaggaat cgaatatatt cccaggccag gtcttttttc tgagagcctt ttaaaattgc
      481 catcgaccac agagcagctg aaccgtggtg aggaccctgg gcttgcaggt caggaggcaa
      541 ctcagactta gtcaacattg ccgccccggc aatgttggat ttggttggat cattaaaagt
      601 gactccatag ctggtgttcc catcatagac cgttgaacga ttctccataa acaattttac
      661 ttcttcggca tattcatagg tatgaacatc cggtggaacg attccttcct ggtaaaattt
      721 ttgcatatag gacaggcctt cgatggttcc tggagtattc acggctggtt ttaagtcatc
      781 agttatcaac cgagaaccaa aatatcgtgc ccagcgatcg aatatttcaa aaatacttcc
      841 aataacacct ctccaggcat aaccaaaaac tttttctcca ttggagcgag taaatgtgcc
      901 cttcttggca atatcaaaaa gttctggtcc agttactgtt ggttgataag ccactccgat
      961 ttcatccaat attttctggt tgaaccactg tatcccgtta taaacaactc gaactggaat
     1021 agcataagga acgtctttta tcttggtaac cgaaagaaga ccttcaggat aatcattggg
     1081 ataatcttcc aagggttttt ctgctaaata tttatccaac ggttcgagga aattggctac
     1141 ccggggcaca aaccagggat caagaacatg gatgatatct tcttcagttc ggttgagagg
     1201 accaagaaga ttcagctttt cctgggtttt tgggttaggg taggtttggt aaacgacttt
     1261 cacattagga tgaagagctt caaattcttc aatcaaatta tcccctttag cgcttggttc
     1321 ttgagatgag gcagcattta aatggacttg atgtccaata ataatcagct cgattttatc
     1381 ctgagcgatt gctccaaacg aaacactgaa tatcacaacc aaagcaacca acatataatg
     1441 taaaatttta ctgttatgca tcattaatcc ttcctttcct tgatctgaat accgtgaaac
     1501 aaaacggtat tatcacaata actcttaaaa tgctcttctc atcaactatt tacctaaaca
     1561 cccccttccc agttattttc gtattactta ccctccttga aaaaccaacc ctcattccga
     1621 atagcaaaaa aggattaaat ataaaatttt ctttctattg actgattcga gggataaatg
     1681 caccctgctc tgctaagact ttttgcaatt ttttcgtatc aactttttta gggataacac
     1741 catcttgaat agatatggca gcagctgttc ccgctgcttc gcccatacca aagcaaaaag
     1801 gcattacccg aatggcaccg agagcctcat gagttgccga tacgcatcgt cctgctacca
     1861 aaagattatc aactttgaga ggaagcaaac atcgataggg aatttcgtaa tggtgtcctc
     1921 ggggtggttc aaataaacca aaagaaccag ctggatcatg aatatcaacc gggaacccag
     1981 cggcaactac acagtcatcg aaaggaacac aattatagac atcttccact tttaatacat
     2041 attcaccctg gatacgtcga ctttctctga caccaacttg atcggctact tcgacgactt
     2101 cagcgttatc acaccctgga ataaaacggc gcatgaattc caaggcttca aaaacttgcc
     2161 tttgtccttc catatgagcc cgggtcaaat cctcaacgtt ggtaccgtca acccccagca
     2221 tcctggtgtg attgatgagc cattcgtccc atcgggtggt ttgataaaga ccgatccggt
     2281 cgtgtgggac tgagtaattc atttctattc tgcctttttt gatgatctct aagaaaccat
     2341 cttcatcttt tcccactggg taactttttc gaacaaaagt gatcaacttt tgagaatcga
     2401 cattcccaat tcgaaagaaa agcgacgacg gctgcatttt tttatccagg tctcttcctt
     2461 tttccatggg aactccactt tgggcggcaa catcaccatc accggtacaa tctatatatc
     2521 tttgagctcg aattgcctgt attcctgatt tgttggcaat aaaagcctct cgaatactgg
     2581 acgcttcgcc ggcagatgaa acctgggttc ctaaaaatcg agaatagagt tttaatctaa
     2641 ctccagcttc ctggcataac tcaaaagcaa ccaatttgaa gacattggga tcaaaagggg
     2701 tcacgtggtc atgtccgatt tgatgaaaac tagaataggg actggaagct ggtatgttgg
     2761 caggatggat agctcctccc tttttctcca ttctgcgaac cacttcatca aaaatcccct
     2821 ttacaatttg gtccttgcca tcgctactga agcaagtcat aaatggccca accagcccaa
     2881 ttgttgccat gcctcctaag ttgccgctat tttcaaccag agttgttttc actccctttc
     2941 tggctgaggc cagtgcactg gcaagaccag ccgtgccacc tccaaccact aatacatcgg
     3001 tttcttctac aacggcttgc tgagtatatg taaccttttc catgaatact ccctccagat
     3061 atatgaaata ttttctcttt gtctattgct tgactgctcc aaaactcatc cctttgatca
     3121 aataactttg taccatccag gtaagaatca acatgggaat aaggtgaatc accgccgctg
     3181 ccaataacat tccccactca actcccatga aagatcccat aaattcagat aaaatgagcg
     3241 gtgcggtggc cgctcttcta ctggtaaaaa taaaggcaaa taagtactca ttccaagcga
     3301 agattgcggt gaaaataacc gctgccacaa tacctggtgc cgatagcgga atcgtgatct
     3361 ttaaaaaggc ctgaaacctt gaacatccat cgatgagcgc tgcctcttct aaggcgaccg
     3421 gtacatcatc aacaaaggtc ttcatcagca acgtggtaat gctcaccatg aaggcagcat
     3481 agagcatgat gagagtgata tgtttatcaa ctaaaccaaa agtattaaaa attggaaata
     3541 atggtatgac cacaattatg ggaggaatca ttctgatgat aatcgtaaat agggttgaca
     3601 tcctcaccag gcggtgagaa aaacgagaaa aagcgtaagc tccgaatatc gaacaaagaa
     3661 tcaccaccaa agctgttcca gaagtcacga taaggctatt caccaaattg ccgaaatatt
     3721 gtggccaatc tttgaccagt tcctgataat tggtgagaga gtaaaaagga ccaaaaaatc
     3781 ttggcgggaa atcccagata tggaggggca tttttaatga gcctatgatc gcattgacaa
     3841 tgggaaagaa agcccacatt accacaatgg ctaacaaaat gccgggaaga attttttcta
     3901 tgagcaagtt tcttctcttg tgggttatca ttttttttca ctccttgcca tcgaaatcgg
     3961 gtgggtttta atatatcttt tcatacatcc ctttatagat ccttctcact aaaggaatcg
     4021 aaatcaatat ggttactatt agcatgatgg tagttgtggc tgctcccctt ccgaattcat
     4081 aataccgaaa ggtttggcga taaagataaa tgggcaaaat ctccgttcct cctgctggac
     4141 caccctcagt gagaatccag attatcccaa attctcgaag agcaaaaatt atcctaaagg
     4201 ttaaaccaac caacagagcg ggtttgatca gttgaaacac tacgaatcga aaggtttgta
     4261 tgcgatttgc cccatcgatt tttgaagcct cgatcacttc ctggggcatg gcggtcaggg
     4321 ctgaataggc aataatcatt ataaaaggga aatttttcca aactgtaaca atgatgatgc
     4381 caataaaaga ggaagttacc gtcccaaacc aggcaattgg tcccaaacca aggaaagaaa
     4441 gagcataatt tacaaatccg tagctcggat caagcatata tctccacata acacacgcta
     4501 caacttcgct gatgcaataa ggggcgataa gcaatgaaac ccaaagtttt tggaggggaa
     4561 actttcggtt caataacaaa gcgcttccca atccaatgat aaattctaaa gcgacgacca
     4621 ctaccacaaa aattatatta tttgctaaag cattcttgaa aaaccgatct cgaataattt
     4681 gaatataatt ttggatatcg ttaaaaatcg gaacattacc tggcgtatat tgggtaaaac
     4741 ttaaaataat cgcccatatc ccagggataa cgataaccac caccaacaac gctatgatag
     4801 gaaccagcat taacatcgaa atattcaatc ttgactttat tctcatcaat cattctcacc
     4861 caccctgttc ttttctttcc acaatcaatc gaagcatgct ctgtgacatc attggccaag
     4921 aatcctttaa atagattcta aagatggtac tttcaacttg gttttcctat ctcctgctaa
     4981 tggaaaaacc attttaattc aactctcttc tcctgactat tctaattttt tacaggtttc
     5041 acctataacc agctcggttg aaagagtgat atccatgcga gatccaacta aattgttcaa
     5101 aatatcataa agtaaccgtg ctgctacccg accgatattt ctttcgccaa tttttattcg
     5161 ggttacactt ttagtaaata acgaattaaa atcttcccct cgatatccgg ctatggaaat
     5221 atcatcagga actcgaattt ttctctcttg aaggtagcga aaaacgccag ccatcatatt
     5281 catatcggtg actaaaactg ccgtaggctg ttccttaaaa gttagtattt tctccattcc
     5341 ccagtaccca ccttgagtcg gatcgccttt ttcttcaata atcagcttgg gatcgacttc
     5401 tattccatgg ttttttaatg aatcttggaa tcctaataat ttatcgtgaa aagaataaaa
     5461 ctcaaggggg ccactgataa gagcgatttt ggtatgaccc agactgatta gatagtcggt
     5521 tagtttatac aaaccaccat aattatcaac caaaacccga taaggtgccg attggtatct
     5581 cccaatcgta accagcagat gaccttttaa aatcagatct tttatcaatt gttcacgaac
     5641 cggtatccca gcaataataa ttccatcaac tatcttctga ttgccataaa aagaaaaact
     5701 cttcttttct ctgatttttt gatgatcagc atatttgatg gttttgagta gtaagctttc
     5761 acgatgttcg tcaacaattt ctccaatgcc tttgagtatt tcaaataaaa ggaaattgtc
     5821 gaacaaggat ccgtagtagg attcagaaat aagagctcca acaatgccca agcgctcgct
     5881 gtgactcccg agccgcttgg caaattcact gggtgaaaag ttcagctgct cgatagcttt
     5941 taaaaccttt tgacgggttt cgggagcaac ccaggatttg ttgttgagaa ccctcgagac
     6001 agttgctttg gaaactccag ctcttttagc tacctcttca atagttattc tcataatatt
     6061 gcctgtaata ttctgttata ttctctgaaa ccgcttttag tcattttttt attgtaagct
     6121 ttctgaaaaa aaaatcaagg ttaggttgaa gtcggttttt ataaagaaaa ttttaggaag
     6181 gtagacattc aagccatttg gtagcactag atgaagatta aagcaaagat tcgacatgcc
     6241 atggcatgtc gctacattaa atgaagatct ggctaggtta atcctgcctg tggatttaca
     6301 gggtaaatta ctaaagatct ttggttgcaa ctggtgcgag agaggggagt tgaaccccta
     6361 cggatttctc cactggatcc taagt
//
LOCUS       DAOSOL010000051         6282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_12519_length_6282_cov_4.349125, whole genome shotgun sequence.
ACCESSION   DAOSOL010000051 DAOSOL010000000
VERSION     DAOSOL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12519_length_6282_cov_4.349125"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK171_02135"
     CDS             complement(<1..566)
                     /locus_tag="PK171_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05035.1"
                     /translation="MSRHSNSLYIWLLLLLFCLVFSQPIYSKINIEYQGQVFSLDLPT
                     IKVNGDNYISVENLFKQIGGVEYYSPIMKKVNLFFGGKNWVLSLDKGIAVAESGEEIP
                     LGRSVVIQDNSVYVSPQFLNQLFGIAIDTSSTTSTQTPSPTKTPSTTSPVTLSTGRSS
                     LLNVDRIPTVMKIVQELLTGPHLGVSVW"
     gene            complement(550..1038)
                     /locus_tag="PK171_02140"
     CDS             complement(550..1038)
                     /locus_tag="PK171_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT05036.1"
                     /translation="MAGDINYFFGIGNLTRDPDLRYTPQGTPVCRFDIAMNRFYQNKN
                     GQQTKDTTFLRITAWSKLGENCARFLHKGSRVAVVGELLANNWEDRNGTKRTSYEVRA
                     MNVQFLSPPQKANEEIRGIGEGFSSEAEIEPFSFDSQNMENALGELEDISPEGNNDVA
                     PF"
     gene            complement(1266..2135)
                     /locus_tag="PK171_02145"
     CDS             complement(1266..2135)
                     /locus_tag="PK171_02145"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT05037.1"
                     /translation="MDSLKVAIIQIQTGPNKKSNLLKTAELFASIKNHQANLILLPEM
                     FQCPYSLESFPTFAEDILTGETSLFLSNLAHEHRVFLVGGSIPEKKGNHYYNTSIVYN
                     PSGEIVGVYRKIHLFDVDFKNLHFQESAVLDPGNTPLLIDTPWGKIGILICYDLRFPE
                     LIRLYALRGAFMVAVPAAFNPVTGPLHWEVLFRARAIDNQVFLLGASPAPHPGSSYQA
                     YGHSLVVDPLGRVLEQLGNEEAIIIKDIDLSLVDQARQQLPLLRHRRQDLYALNPDEK
                     LVSLLKDQKGGAI"
     gene            2284..3345
                     /locus_tag="PK171_02150"
     CDS             2284..3345
                     /locus_tag="PK171_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05038.1"
                     /translation="MKCFLFIPENDLEDQISKNICWNLRKVSLAKVYFFHPHYIQELK
                     KQDYPEIKSDITLPSIFQLFPKVIEWYRMIRTLRRRRRFRETIILIGHKTCLLLWIFF
                     LIQKKPIFIWITRQFNQTFSWFDYYFTKYLTQSFLTNDQYLNHYLRQKNQASYFIGNV
                     LSDLVQPIEFPFIHGKKPIYSFFPSIDRFEFDFELILKLFPDFADMMNGYYLMVIPPG
                     QKTMQAITAIASRLGWNLSKSLEGEILEGYLWKNPYYINLTLFQSETLVQSDLILSMD
                     DLYTIQAVGINKKVIPLDRNHPQEMFVYLKNTNNFYEYNNFLKSRFGTTGTIERIAAY
                     LLWGVVEDQRFLRNKTTPN"
     gene            3440..4300
                     /gene="nadC"
                     /locus_tag="PK171_02155"
     CDS             3440..4300
                     /gene="nadC"
                     /locus_tag="PK171_02155"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOT05039.1"
                     /translation="MRGLVPWDVDRRLQIFLEEDLGFDDITTQGLGDLSLKPVRARII
                     AKEKGIMSGGPFAIRLFNLIDPQTNEKTLIEEGEEFKSGDCVMEIIGQTRAILMGERV
                     ALNLLQRLCGIATKTREFVEAVKGLPVKIADTRKTSPGLRIFEKYAVRCGGGVNHRLG
                     LYDCALIKDNHISICGSVKDAIQRVRPAIPFTARIVVECENILQVSEAIEEKADVIML
                     DNMNIGQIKEAVKMINKRSIVEASGGVSLETVREIAKQGVDIISTGYITHHAIWLDLN
                     LEVEEVKDVL"
     gene            4290..5855
                     /locus_tag="PK171_02160"
     CDS             4290..5855
                     /locus_tag="PK171_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT05040.1"
                     /translation="MYYDALIIGNGIAGSSAALFLAEKSQRVLLVSKGQSLEETNTEK
                     AQGGIVYRGKNDSWKILEQDILNASDGSALEKSAKVLAKIGPSLVKRLFIQKLRVPFE
                     ENEQGEWDLFQEAAHSQRRVLHVKDCTGRAIQKALHEGILEEHLIEVKKNTYAIGLIL
                     SNRYQFSSRSRYETPECLGVYLLDPRKRTIIPFFARATIFATGGLNSLYQYSTGGPWN
                     TGDGYAIANRAGAILQNMEYVQFHPTLLFSPEYSQTFLISETVRGEGAELVNHEGKAF
                     MHKYHHLGSLAPRDVVSRAIFEEMLTQNSHCVFLDLCHVISPEKIKATFPTIYEECLY
                     RGIDITREPIPVAPGAHFCCGGILTDSFGRTSIKRLYAIGEVACTGVHGANRLASTSL
                     LEGLTFAYRASHDIIKNPATKEQPRIIPFEYSCGNPPPEAILESMRKIIQDTMWKFAG
                     LIRDQSGLKYALEVLRGLKKQAVQLKNQLGVSGSLLEFENSVDSGLLVVEAALRNPIS
                     LGTHFRSDSSMVQ"
     gene            complement(5880..>6282)
                     /locus_tag="PK171_02165"
     CDS             complement(5880..>6282)
                     /locus_tag="PK171_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05041.1"
                     /translation="GETYFFFDPKDTNIFGLELVYGNLDQTYLRLRGEYDLNIGGLGD
                     IIAGIGLGSKEFSVGMETIYSFPDQNWEERRYYIRKQLEECIDIEASYWESDQTFFVS
                     VNLAGLDQKKHIESLFDEQEEYDLFNLNREN"
BASE COUNT         1990 a         1138 c         1239 g         1915 t
ORIGIN      
        1 ctccagacag atacccccag gtgaggacct gtgagtaact cttgaacgat tttcatcacc
       61 gtaggaatgc gatcaacatt aaggagcgac gatcttccag ttgaaagagt aaccggtgag
      121 gttgtagatg gagtttttgt tggtgaaggt gtctgggttg aggttgttga gcttgtatca
      181 atggcaattc caaagagctg attaagaaat tggggtgaaa cataaacaga attgtcttga
      241 atgactaccg aacgtcctaa gggtatttct tcaccacttt cagcaacggc tattcctttg
      301 tcaagtgata aaacccaatt ttttccccca aaaaatagat ttactttttt cataatggga
      361 gaataatatt ctaccccccc tatttgtttg aaaagatttt caaccgagat gtaattatca
      421 ccattgactt ttattgttgg taaatcaagc gaaaagacct gtccttgata ctcaatattg
      481 atttttgaat agattggttg agaaaaaacc aagcaaaata gaagaagcaa aagccatata
      541 tataaggaat tagaatggcg cgacatcgtt gttcccttct ggagaaatat cttctaattc
      601 tcctaaggca ttctccatat tttgggaatc aaaactaaaa ggttcaattt cggcttcaga
      661 tgaaaaaccc tcaccaattc cccgaatttc ttcgttggct ttttgaggcg gggaaagaaa
      721 ttgtacattc attgcccgga cttcatagga agttcgttta gtcccgttcc gatcttccca
      781 attattagca agaagctctc caactaccgc tactctgctc cctttatgaa gaaaacgtgc
      841 acaattctca ccaagcttac tccaagcggt tattcttagg aaagtggtat ctttcgtttg
      901 ttggccattt ttattttgat aaaaacggtt catagctata tcaaatcgac atacgggtgt
      961 cccctgggga gtatagcgaa gatcagggtc acgcgtgaga tttccaatac caaaaaaata
     1021 gttaatatct ccagccattt ctgttccccc atataaatta agaaattcta aataaataat
     1081 ttctgttagt ttagcatatt ctaaaccata aatacaatga cgaatgatga tgaaaagatt
     1141 ctatcaccct gaggatatct cttttaaatc cgagaagatc tcgtcttttc tttttttcac
     1201 tcttcttgct gaggaatccc ttgctccctt tattaagggg agtaagggat tttagggacg
     1261 atgatttaaa ttgccccccc tttttgatct tttaataaac taactaattt ttcatcagga
     1321 tttagtgcat agagatcttg tcggcgatgc cttaacaaag gaagttgttg gcgggcttgg
     1381 tcaactaaag agagatcaat gtcttttatt attattgctt cttcattacc aagctgctct
     1441 aaaaccctac ccaaaggatc aacaacaagg ctatggccat aagcttgata ggatgagcct
     1501 gggtgtggtg ctggtgatgc gcccaaaaga aaaacctgat tatcaattgc ccgcgctcgg
     1561 aagagaacct cccagtggag aggcccggta accgggttaa aagcagcggg gactgcaacc
     1621 ataaatgccc cccgcaaagc ataaaggcga ataagttcag gaaaacgaag atcgtaacag
     1681 attaaaatac caattttccc ccaaggagta tcaattaaaa gtggagtatt tccaggatcg
     1741 agaactgctg attcttggaa atggaggttt ttaaagtcaa catcgaaaag atgtattttc
     1801 ctataaacac caacaatctc accagagggg ttataaacta tagaggtatt ataatagtga
     1861 tttccttttt tttccggtat tgaaccgcca actaagaaaa ctctatgttc atgagcgaga
     1921 ttcgataaaa agagactggt ttcgccagta aggatatctt cagcgaaagt tgggaatgat
     1981 tccagactat aagggcactg aaacatttcc ggtaggagta tcaagtttgc ttggtgattc
     2041 ttaattgaag caaaaagctc ggcagttttt aacaaattag attttttatt tggaccggtt
     2101 tgaatttgaa taatcgcaac ttttaaacta tccatagccc tatctcaaag aattatttta
     2161 aaaaaagcaa ggtttactta gaaaagctgt tctcagcctt ttcaataata tagtacaata
     2221 attgaagcat tggcaagagt atatttttat agcttataaa atctttataa gcgaggggaa
     2281 aatatgaagt gttttctatt cattccagaa aatgatttag aagatcaaat cagtaaaaat
     2341 atctgttgga atttaagaaa agtatccctt gccaaagtct attttttcca cccccattat
     2401 atacaagaat taaagaaaca agattatcct gagataaaat ccgacataac tctaccatcg
     2461 atatttcaat tgtttccaaa agttattgaa tggtatcgta tgatacgaac cttgcgtcga
     2521 aggagaagat ttcgagagac tataatattg ataggacata agacttgtct tcttctatgg
     2581 atattttttc tcattcaaaa aaaaccaatt ttcatatgga taactcgtca atttaaccag
     2641 acattctcat ggtttgatta ctattttact aaatacctta cccaatcatt tttaaccaat
     2701 gatcaatatc tcaatcatta tctccgtcaa aaaaaccaag cttcttattt cattggaaat
     2761 gttctttctg atttggttca acccattgaa tttccattta tacacgggaa aaagccaatt
     2821 tattcttttt tccccagtat tgatcgtttt gaatttgatt ttgagcttat cctcaaactc
     2881 tttcctgatt ttgccgatat gatgaacggt tattatctga tggtaattcc ccctggtcaa
     2941 aaaaccatgc aggcaattac tgccatagca agtcgattgg gatggaattt atcaaaaagt
     3001 ttagaaggag aaatattgga aggatatctt tggaaaaacc catactatat taatttaact
     3061 ctttttcaga gcgaaacctt ggttcaatcc gatcttattt tatcaatgga tgatctttac
     3121 accatacaag cggttggtat aaataaaaaa gtcatacctc tggatcggaa tcatcctcaa
     3181 gaaatgttcg tttatttaaa aaataccaac aatttttatg aatacaacaa ttttttaaaa
     3241 agccgatttg gaacaacagg aaccatagaa aggattgctg cctatcttct ttggggagtt
     3301 gttgaagacc aaagatttct aagaaataaa actacaccaa actgaaaacc atatatatat
     3361 caataaaaaa aagaaagaag gaacaatcca tgataaaata aatagaattt atttttagaa
     3421 tgggaaggat gatgaacgag tgagaggttt ggttccatgg gacgtcgacc gaaggttgca
     3481 aatattttta gaagaagatt taggttttga tgatattact acccaaggtt tgggagacct
     3541 tagcttaaag ccggtgcggg caagaattat tgctaaagaa aaaggaataa tgtcaggagg
     3601 tccatttgcg attcgccttt ttaacctcat agaccctcag acgaatgaaa agactcttat
     3661 tgaagaaggt gaagaattta aaagtggtga ttgcgttatg gaaattatag ggcaaacccg
     3721 ggcaattttg atgggagaaa gggttgccct taatttatta caaagactgt gtggaattgc
     3781 tacaaaaact cgagagtttg tcgaagcggt taagggttta ccggtaaaaa tagctgacac
     3841 aagaaagact agtcctggtt tgaggatctt tgaaaagtac gctgtccgtt gtgggggtgg
     3901 agtaaatcac cgtctcggct tatacgattg tgctcttatc aaggataacc atatttcgat
     3961 ttgtggatcg gtaaaggatg ccattcaaag agttagacct gctattccct ttacggcacg
     4021 aatcgtggtt gaatgtgaaa atatactaca agtgagtgag gcaatcgaag aaaaggctga
     4081 tgttattatg ctggacaata tgaatatagg acaaattaaa gaagcagtaa aaatgattaa
     4141 taaaagatcg atcgtagagg cttcgggagg tgtcagttta gaaacggtaa gggaaattgc
     4201 aaaacaaggt gttgatatca tctctactgg atatattacc catcacgcca tttggttgga
     4261 cttgaatttg gaggtagaag aagtcaaaga tgtattatga tgctttgatt attgggaacg
     4321 gaattgcagg aagtagtgct gccctttttc tggcagaaaa atctcaacga gttttattgg
     4381 tcagtaaggg acaatcgctt gaggaaacta acaccgaaaa agcccaggga gggattgtat
     4441 atcgaggcaa aaatgattct tggaagattc tcgagcaaga tattcttaat gcttctgatg
     4501 gaagtgcctt agaaaaaagt gctaaagtgt tagctaagat tggtccttcg ctggttaaaa
     4561 gattatttat tcaaaaacta cgggttccat ttgaggaaaa tgaacaggga gaatgggatc
     4621 tttttcagga agcagcgcat tctcaaaggc gtgttcttca tgttaaggat tgtactggaa
     4681 gagctattca aaaagcactt catgaaggaa tcctcgagga acatcttatc gaggttaaaa
     4741 aaaacaccta tgcaattggc ttgattcttt ccaatcgcta ccaattttca tctcgatctc
     4801 gctatgaaac tccggagtgt ctgggtgttt atttgctgga tcctcgcaaa aggactataa
     4861 tacccttttt tgctcgggca accatttttg ctactggagg attaaattct ctttatcaat
     4921 attcaactgg aggaccttgg aataccggtg atggttatgc tatagcgaat cgagctggag
     4981 ccatcctgca aaatatggaa tatgttcaat tccaccctac ccttcttttt agtcctgaat
     5041 attcccagac ttttcttatt tctgaaacgg ttcgaggcga gggagctgaa ctcgttaatc
     5101 atgaagggaa agcttttatg cataaatatc atcatttggg aagcttggcg cctcgtgatg
     5161 tggtttcccg ggcaatcttt gaagaaatgc tcacccagaa ttcccattgt gtttttcttg
     5221 atctttgtca tgtgatttca ccggaaaaaa taaaggcaac cttcccaacc atctatgaag
     5281 aatgtttata ccgagggatt gatattaccc gagaaccgat accagttgcc ccaggagctc
     5341 atttttgctg tgggggaata cttaccgata gttttggccg tacctcgatt aaacgtctct
     5401 atgctattgg cgaagttgcc tgcactggtg tgcatggtgc caatcgcttg gcttctacca
     5461 gtcttttaga aggattaacc tttgcttacc gagcttctca tgatattata aaaaatccag
     5521 ctaccaagga acaacctcga ataataccgt tcgaatattc ttgcggaaat cctccacctg
     5581 aggcaatttt ggaaagtatg cggaaaatta ttcaagatac catgtggaaa ttcgccggac
     5641 ttattcggga tcaatcgggg ttaaagtacg ccttagaagt gcttcgtggt ttaaaaaaac
     5701 aagcagttca actgaaaaac caattaggtg tatctggctc tctccttgaa tttgaaaata
     5761 gtgttgattc cggtttattg gtcgttgaag cagccttaag aaatccaatc tctttgggaa
     5821 ctcattttcg ttccgattct tcaatggttc aatagttatt aatcttgttt tatcatttat
     5881 tagttttcac gattcagatt aaaaaggtcg tattcttctt gctcatcaaa taagctctcg
     5941 atgtgttttt tctgatcaag tccagccaga ttaacactta caaaaaaggt ttggtcagat
     6001 tcccaataag aagcttcaat atcgatacat tcttctaact gttttcgaat ataataacga
     6061 cgctcttccc agttttgatc gggaaaggag taaatagtct ccatgcctac agaaaactct
     6121 ttacttccta atccgatacc ggcaataata tcaccaaggc ctccaatatt aagatcatac
     6181 tcgcctcgaa gtcgaagata agtttggtca agattaccat aaaccaactc caaaccgaaa
     6241 atattggtat ctttgggatc aaagaaaaaa taggtttctc ca
//
LOCUS       DAOSOL010000052         6261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_12585_length_6261_cov_3.799388, whole genome shotgun sequence.
ACCESSION   DAOSOL010000052 DAOSOL010000000
VERSION     DAOSOL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12585_length_6261_cov_3.799388"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..841)
                     /locus_tag="PK171_02170"
     CDS             complement(113..841)
                     /locus_tag="PK171_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOT05042.1"
                     /translation="MVTWYSGISLLYILKLRYNNYMCGRFALIITPAELEKIFGLRLD
                     EEFNPRYNIAPSQTVPVITYLEKDNKKKLSSMKWGLVPSWAKDPSIGDRMINARSETI
                     QEKPSFRSAFQRRRALIPASGFFEWKREGTTKNPYFIGMKEMKTFAFAGLWERWVHDN
                     NFLETFTILTTEANELVRLIHDRMPVIMPESVYDIWLYPSTDISQIIPLLAPYPENKM
                     QAYPISKLVNNPKNDGPEILERVG"
     gene            948..1934
                     /locus_tag="PK171_02175"
     CDS             948..1934
                     /locus_tag="PK171_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05043.1"
                     /translation="MFKKLSVFSLLLVLVLVMGTVVFAQEKEAGELYFRFVTHGGDDP
                     FWAVVVKGAQDAAKLLNCKVDFDLVGGDLALQQKRMQEAIAMNVDGIALVINDDTVWD
                     KLVVDALAKGIPVVAIDNDDTEGDRGNSRLTYIGQDEFKAGYDLAMRLFEEGKKKGLD
                     LSQAHVAMSVEVPGAMYGVVRSDGVKKAMEEFGITSSEIIDAGGLEMTTVEQRLTSYL
                     ISHPEATFIIGLGGIVTDRLTDALKAVGRQAGEVIAGGFDMTPGTLVGLKEGYMTAAI
                     DSQQYLAGFYGVLVLYHYKLYGFLPTVKTGGFLIDSPEKIEQIEVLSKDYIR"
     gene            complement(2008..2700)
                     /locus_tag="PK171_02180"
     CDS             complement(2008..2700)
                     /locus_tag="PK171_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOT05044.1"
                     /translation="MVYPRTKLLSFVWIISLMTLLVMFMSFLANGNELVTLPQIEGND
                     NDLISAIINRKAERQFAAETIALKDLALILWAGSGIKDPQVDSVSHATRTIPSAMGIY
                     PIGVYVFALQVENLPSSIYLYLPEKHALQEIPSVNVTEALTKITNQRAVQNASLVFLI
                     AFHRDKSSRMNDKFAYFEAGEVVQNISLMAVDRGLGSYVIGMYNQEKIIEVLNEENIE
                     PVVLMAVGKPSQ"
     gene            complement(2725..3621)
                     /locus_tag="PK171_02185"
     CDS             complement(2725..3621)
                     /locus_tag="PK171_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT05045.1"
                     /translation="MIVLSQEAAYWVAIAHLTNWSREKINDFFDRLLRNHFAFHDFFT
                     LKPEEWESLFHLSREEITSLQSIQEKLPYYLTITQKISEQGFDIIPVHSRFYSAHLRE
                     KLGKSHAPTLFYTKGNVDLFNTPTACILGSNRGSGNGLIFIQNLIRRFVQEEITMITS
                     IEPGYHRYILNLCLQFGGRSLVILDQGVLSLKRPLDDFDQYLINDQVLFLSTIFPKNN
                     NGKKWALQRDLLQYGLAKDIYIADALEKRQIWRWILRGLRQNKTFFVRYPEENEKSAN
                     RFFIANGAIPVDLNGRIINKSD"
     gene            complement(3671..4618)
                     /locus_tag="PK171_02190"
     CDS             complement(3671..4618)
                     /locus_tag="PK171_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05046.1"
                     /translation="MRDQVVRAIRRDHPDYVPLIIWNQDFDQSDIVYGEIQKHFLGEN
                     RDYSEWGFYWSRKDETMGQPREPLIKDWNEIDKLKPPSLDLPGRFDELLKKKETYPEK
                     FLMASLALSGFTAMTFLAHFEEVLIHLSTKSQQLERLIDVVFNFEESLIRETSNFDVD
                     AIAFLDDWGSQNGLMVSPDHWRKIFKPRYRKQFDLVHQQGMYVYFHSCGYIYPIISDF
                     IEIGVDILNISQPNLYEIPKLGRDFGGKVCFCCPVSYQTTGISGNPQEIEQYIEHLFE
                     HLGRFNGGLIGYAEEYHSIGMSEENYRASIRGFKKLHYF"
     gene            complement(4650..4997)
                     /locus_tag="PK171_02195"
     CDS             complement(4650..4997)
                     /locus_tag="PK171_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT05047.1"
                     /translation="MIRTKLSKVKEIRTPHGKKVRWLISKEMGAPRFEMRHFTITDES
                     QPSEEAHPWEHQVYILSGEGIIKSGDTEIKVEPGDAIYIAPNEPHLVQNLPQQKFTFL
                     CIIPAGCEDRVKG"
     gene            complement(5021..6013)
                     /locus_tag="PK171_02200"
     CDS             complement(5021..6013)
                     /locus_tag="PK171_02200"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOT05048.1"
                     /translation="MIYIINDSQDPYFNLASEEYIMNTFHVDEKYLMLWQNQPSIIIG
                     KHQNTIEEINNEFVQENNIAVVRRLSGGGAVYHDLGNLNFTFIVKASDNSLDFDFKKF
                     TEPVITALNRMGIPAEFNSRNDLSIEGKKFSGNAQYIRKGKCLHHGTLLFNTNLDHLE
                     MALRVSSDKIESKGIKSIRSRVTNIYPYIKKTCSIEEFKNLLYDQFCLINEEIQSIYF
                     SENDIKKIEELANSKYRTWEWNYGESPFFTIKKSHRFEFGKVEAWLDVNQGIIRQCKF
                     YGDFFGREDKADIEKLLVGSRYERNDIAFRLRNVQIGSYFHGLNTEQLIKLLIS"
BASE COUNT         1834 a         1258 c         1178 g         1991 t
ORIGIN      
        1 atttcctcgg tccaggtatt tcggttagtc atcgactttc ctccttcatt attgaataaa
       61 attaaaatat taatctcatt tttttatagg ctattttgta tataaatgtg cactatccca
      121 ctctttcaag aatttctgga ccatcgtttt tagggttgtt aactaatttt gatatgggat
      181 aggcttgcat tttattttcg ggatagggcg ctaaaagcgg aataatttga gatatatcag
      241 tagatggata aagccatata tcgtaaacac tctcaggcat aataactggc atacggtcat
      301 ggattaggcg gaccagttca tttgcttcag tggttaatat ggtaaatgtt tccaagaaat
      361 tattatcgtg tacccatcgc tcccataatc ctgcaaaagc aaaggttttc atttctttca
      421 ttccaataaa atagggattt ttggtagttc cttcccgttt ccattcaaaa aaaccactgg
      481 cgggtatcag cgctcttctc ctctggaagg cgctgcgaaa cgagggtttt tcttgaatgg
      541 tctcggaacg ggcgttaatc attcggtctc cgatagaagg atctttggcc caggaaggta
      601 ctaatcccca tttcatgctg gatagctttt tcttattatc cttttccaaa taagtgatta
      661 ccggcacggt ttgactagga gcaatattat accgggggtt gaactcttca tccaatctca
      721 acccaaaaat tttttctaat tctgccggtg taataatcaa ggcaaatcgt ccacacatat
      781 aattattata ccgaagcttt aaaatatata aaagggatat gccgctatac caagtaacca
      841 aacctcttta tcttgacctc ctcccctggc tttccatcat ttccaagttt gcaaaaaaat
      901 gaaaatatgg cacaataata aatatcaata taataggagg gacataaatg tttaaaaagc
      961 tctctgtttt ttctcttctt ttagtgttgg ttttagttat gggaacggtg gtttttgctc
     1021 aagaaaaaga agctggagaa ttgtattttc gctttgtaac ccacggagga gacgatccct
     1081 tttgggcggt agtcgtcaag ggagcccagg atgcagctaa actactcaac tgcaaggtgg
     1141 atttcgattt ggttggtgga gaccttgccc tgcaacaaaa aagaatgcaa gaagcaatcg
     1201 ctatgaatgt cgatggaatt gccctagtta ttaatgatga tacggtttgg gataaattag
     1261 tggtagatgc tttagccaag ggtattccag tagtagccat cgataatgat gataccgagg
     1321 gtgatcgagg aaactcgcgc ttaacttata ttgggcagga tgaattcaaa gctggttatg
     1381 atttggcaat gcggctcttt gaggaaggga aaaagaaagg attagacctc agccaagctc
     1441 atgtagcgat gtcagttgaa gttccaggag cgatgtatgg tgttgttcgt tccgatggtg
     1501 ttaaaaaagc tatggaagaa ttcggtatca cttcatcgga gatcatcgat gccggtggtt
     1561 tggaaatgac taccgttgag caaagactga cctcttatct catttcccat cccgaagcaa
     1621 cctttataat cggcttgggt ggaattgtaa ccgatcgatt aaccgatgcc ctgaaagcgg
     1681 ttggaagaca ggctggtgaa gttatcgctg gaggtttcga tatgactccg ggaacgctgg
     1741 ttggattaaa agaaggttac atgaccgctg cgattgattc acagcaatat ctagctggtt
     1801 tctacggggt gttggtcctc tatcattaca aactttacgg tttccttcca accgtgaaaa
     1861 ccggtggatt cctgatcgat agcccggaaa aaattgaaca aattgaggtt ctttctaaag
     1921 attatatccg gtaaatactt aatcattgca aaaaaccgcg gtttaagaaa ggtttccccc
     1981 ttaaccgcgg ttttttttat ttattgatta ttgggaaggt tttcccaccg ccatcaatac
     2041 aactggttca atgttttctt cattcagaac ctcgattatt ttttcttggt tgtacatacc
     2101 aataacatag cttcccaatc cacgatctac ggccatcaaa ctgatatttt gaaccacctc
     2161 accggcttca aagtaagcga atttatcatt cattcgggaa gacttatccc gatgaaaagc
     2221 gattaagaaa accagagagg cattctgtac tgctctttga ttggttattt tggttaatgc
     2281 ctcagtcaca ttgacgctcg gaatttcttg gagggcatgt ttttccggca aatacaggta
     2341 aatactggat gggaggtttt caacctgtaa ggcaaataca tatacaccga tgggatagat
     2401 tcccatggct gaaggaatgg ttcgggtagc atgagaaacg ctatcgacct gagggtcttt
     2461 tatcccactt cccgcccaga gaatcagagc cagatccttc agcgctatcg tttcagctgc
     2521 aaactgtcgt tctgcctttc gattgattat agctgagatt aaatcattat cattgccctc
     2581 aatctgggga agagtgacga gctcatttcc atttgctaaa aagctcatga acatcaccaa
     2641 caaggtcatc aaagaaatta tccagacaaa agaaagaagt ttggttctcg gatataccat
     2701 agttaaacct cctgtgattt taaatcaatc agatttattg ataattcttc cattaagatc
     2761 aaccggaatt gcgccattag cgataaaaaa acgattggcg cttttttcat tttcttcggg
     2821 atatctgaca aaaaaggttt tattttgcct caaccctctt aggatccacc tccatatttg
     2881 ccttttctca agagcgtcag caatgtagat gtccttagcc aatccatatt gtaacaaatc
     2941 ccgctgcaga gcccatttct ttccgttgtt gttttttgga aagatagtac tgaggaatag
     3001 cacttggtca tttatgagat attgatcgaa atcatccagc ggtcttttta aagaaagcac
     3061 tccctggtcc aaaatgacta aagatcgccc tccaaattgg agacataaat tgagaatata
     3121 acgatgataa ccaggttcaa tgctggttat catcgttatc tcttcttgaa cgaaacggcg
     3181 tatgaggttc tggataaata tgagcccgtt tccagaaccg cggttcgaac ctaaaataca
     3241 cgctgttggc gtattaaaaa gatcaacatt ccctttggta taaaagagag ttggagcatg
     3301 agattttccc agcttttctc gtaaatgcgc agaataaaac cgagaatgaa ctggtatgat
     3361 atcaaagcct tgttcagaga ttttttgggt aatagtcaaa taataaggga gtttttcttg
     3421 tatcgattga aggcttgtta tttcttcccg ggaaagatga aaaagactct cccattcttc
     3481 aggtttcaaa gtaaaaaagt catgaaaagc aaaatggttc ctcaataatc ggtcaaaaaa
     3541 atcattgatt ttttctctgc tccaatttgt aaggtgagca attgccaccc aataggcagc
     3601 ttcttgagaa agaacaatca tatcaatgac ctcgtttttt tattataata attgtctgcc
     3661 tcttttttct ttagaaatag tgtaatttct taaatcctcg aatactggct cgataattct
     3721 cttcgctcat tccaatcgaa tgatattctt cagcgtaacc tatcaaccct ccattaaatc
     3781 tccccaggtg ttcaaataag tgttcaatat attgttctat ttcttgaggg tttccagata
     3841 taccagtagt ttgataactc actggacaac aaaaacaaac cttccctcca aaatctcttc
     3901 ctaacttcgg aatctcatag agattcggtt ggctgatatt gagaatatcg actccaatct
     3961 cgataaaatc tgaaatgatt gggtaaatat atccacaaga atggaaatag acatacattc
     4021 cttgctgatg aactaaatca aactgttttc ggtatcgtgg tttgaatatt ttcctccaat
     4081 gatcaggaga aaccatcaaa ccattttgtg aaccccaatc atccaaaaaa gcgatagcat
     4141 ccacatcaaa attcgaggtt tctcttatca gactttcttc aaagttaaat accacatcga
     4201 tcaatcgttc caactgttgc gatttagtcg ataaatgaat caacacctct tcaaagtgag
     4261 ctaaaaaagt catcgcggta aaccctgaca aagccaaact ggccatgaga aatttctccg
     4321 gataagtctc tttctttttt aagagttcat caaatcgacc gggtaagtct aaggatggtg
     4381 gcttaagttt atcaatttcg ttccaatctt ttatcaaggg ctctcgcggt tggcccatgg
     4441 tctcatcttt tctcgaccaa taaaatcccc attctgaata gtctcgattt tctcctaaaa
     4501 aatgtttctg aatttcaccg tagacaatat ccgattgatc aaagtcttgg ttccagatga
     4561 ttaaaggaac atagtcggga tgatctcttc gtatagctcg aacaacttga tccctcatta
     4621 aaattcaact ccttcggaat aaaataacct taccctttaa ctcgatcttc gcatccagcc
     4681 gggataatgc aaagaaaagt aaatttttgt tgagggaggt tttggactaa gtgcggttca
     4741 ttaggagcaa tataaatagc atcccccggt tcgaccttga tttcagtatc tccactttta
     4801 atgattcctt ctcctgaaag aatgtaaacc tgatgttccc agggatgagc ctcttctgag
     4861 ggttgactct catcggtgat ggtgaaatgt ctcatttcaa aacgaggagc gcccatttct
     4921 tttgaaatca accagcggac cttttttcca tgcggggtcc tgatttcttt aaccttgctc
     4981 aatttggttc gaatcatatt catgctcctc tctttattaa ctaagaaatc aatagtttga
     5041 taagctgctc ggtattcaat ccgtgaaaat agcttccaat ctgaacattc cgcaacctaa
     5101 aagcgatgtc atttctctcg tagcggctgc caaccaatag cttttcaata tcagctttat
     5161 cttctcttcc aaaaaaatca ccataaaatt tgcattgtcg gattattcct tgatttacat
     5221 ccagccaggc ttcgactttg ccaaattcaa atcgatgaga ttttttaatc gtaaaaaagg
     5281 gtgattcacc ataattccat tcccaggttc ggtatttgct attggctaat tcctctattt
     5341 tcttgatgtc gttttcactg aaatatatcg actgaatttc ttcatttatt aagcaaaatt
     5401 gatcataaag tagattttta aattcctcta ttgagcaggt tttttttata taggggtaga
     5461 tattggtaac tcgactccga atggatttaa tcccttttga ttcaatctta tcgcttgata
     5521 ccctcagtgc catttccaag tgatctaaat tggtattgaa tagcaaggtc ccgtgatgaa
     5581 gacattttcc cttccggata tattgtgcat ttccagaaaa cttctttcct tcaatagata
     5641 aatcatttcg actattgaat tcagccggga ttcccattcg attcaaagca gtaatcactg
     5701 gttcagtaaa ttttttaaaa tcgaaatcca atgaattgtc tgatgctttc actataaacg
     5761 taaaattcag attgcctaaa tcgtgatata cggctccccc accggataat cttctcacca
     5821 cggcgatatt gttttcctgg acaaattcgt tattgatttc ttcaatggtg ttttgatgtt
     5881 ttccaatgat tattgatggt tggttttgcc aaagcatcaa gtatttttcg tcaacatgaa
     5941 atgtattcat gatatactct tcgcttgcta aattgaaata gggatcttga gaatcgttga
     6001 taatataaat catacaatct ccttccaggt attaatatta actttcataa ttttactgaa
     6061 atattcaatc ctgtaaaccc atttatgaac tttataaatc aaacctctat cctgaaaata
     6121 ccggaataag tgaaaagtga tcaaaccaaa aagagaaaga atggattcaa caattcccct
     6181 ccttggaggg gtgccgtttt ccggcgggga gggtgccttt aattcgtcat cctgaggctt
     6241 cgtattcaga aaccgtgagg a
//
LOCUS       DAOSOL010000053         6218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_12698_length_6218_cov_3.967061, whole genome shotgun sequence.
ACCESSION   DAOSOL010000053 DAOSOL010000000
VERSION     DAOSOL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12698_length_6218_cov_3.967061"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..275
                     /locus_tag="PK171_02205"
     CDS             99..275
                     /locus_tag="PK171_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05049.1"
                     /translation="MKKYIFGYFPLVILAYLIPYTLLQKVHKFYGSFLFWIGYTLIVI
                     YLMKKMMDRWKNEN"
     gene            265..1725
                     /locus_tag="PK171_02210"
     CDS             265..1725
                     /locus_tag="PK171_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOT05050.1"
                     /translation="MKIELIWISIIIYMAWGTLIAVLSRRGGASGVVDYFLADRKLKG
                     YISSLSYSATTYSAFMLVGLAGLTYKGGVGALGFELIYLSGLFWAVLFGPFYWRAGIH
                     YNCVSPAELLSNRYQNKKVGALSAWISLIFLIPYSSIQLMGIGYLLETLSNGKIPFLW
                     GALIATLITLVWTEIAGLRSVATTDSLQAGIMLISSIIFIFILIGKFLGGFDNFLYRI
                     ETNYPLWLSVPGNGYFSFQTFLGLSLPWFFFSISNPQVSQRLFVPSSLSKMRSMINGF
                     LGYGFVYTLITILIGFCALLLFPGLSNPDQATPTLLAQLNIPPIIVLLMIVGIFSAAI
                     STIDSVMLTISSMFVRDIVRARREIPEKQQLVIARWFILILGVGIFLFAIQRFNLIAV
                     LSVASSAGLLATVPSTIGGFLWKKGSASGALISMIGGACISLILQITSWKPWGWWPGV
                     WTIIIATLLYIIMSYAKPAAINQEFFKVIQSQPKKT"
     assembly_gap    2120..2129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2220..2867)
                     /locus_tag="PK171_02215"
     CDS             complement(2220..2867)
                     /locus_tag="PK171_02215"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT05051.1"
                     /translation="MEIDNVRTIQEREEMVRKQIISRNIKDERVTQALLKIPRHVFVP
                     EFYRKYAYNDTPLVIGEGQTISQPYIVALMSELLEIQPTDRILEIGTGSGYQTAVCAE
                     LGKEVYTIERIEELAQKAEEVLESLEYRNIHFFVGDGSRGIPEYAPFDKIIVTAYANE
                     IYPVWVEELKEGGIIVLPLGGDFRQTLVRGRKQKGSLKIEKHGSVVFVPLIEDIL"
     gene            complement(2867..3145)
                     /locus_tag="PK171_02220"
     CDS             complement(2867..3145)
                     /locus_tag="PK171_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOT05052.1"
                     /translation="MKFIQGRLVFKGRVQGVGFRYFVLKRASQFEVTGFVKNLFPNGV
                     EVVAEGEKSEVEHFFQEIREGPVSASIRDIFEEWLPYSGNYESFQVEY"
     gene            complement(3147..3920)
                     /gene="surE"
                     /locus_tag="PK171_02225"
     CDS             complement(3147..3920)
                     /gene="surE"
                     /locus_tag="PK171_02225"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOT05053.1"
                     /translation="MKRNILITNDDGYQSDGIMALVRVFKPIANIILVAPLEERSCSS
                     HSITARQTLRVKDVQVDGVSGLAVDGTPADTVILGLNLFSQTPVHFVISGINRGPNIG
                     FDVFYSGTVGAAMEAAMSGIPAMAVSLSIQEKLNYKVAAETALDIFLHFEETLSRQKN
                     LVLNVNIPDVTHSDELKGWKITELADRFYFTKVQKTIQNEEFQEYVFIEEKRQIEFQS
                     SSDYWSVVNSEVSITPLRPNLTDWLVKNELVEVLSEKNN"
     gene            complement(3917..5311)
                     /locus_tag="PK171_02230"
     CDS             complement(3917..5311)
                     /locus_tag="PK171_02230"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT05054.1"
                     /translation="MKNTYDVIIIGGGPAGIFAALELADKTDLDILIIDRGGDIEERR
                     CPAREKKNICQKCQPCSLLCGWGGAGAFSDGKLTFSHDVGGQLTSYLKPNDLEQLLQY
                     VDNIYLRYGATPRVYGDDGEKVEELRKKAEMAELILLPSRVRHLGTDSCRGILTRIKN
                     HLKEKVTILTRTEAAEILTKNHQAVGVQLKNGDVIKSHYLIVAPGRVGADWLRTEALK
                     LKLDLVNNPVDLGVRMEVPASIMEEYTKVLYELKLIYYSHTFDDRVRTFCMCPHGEVV
                     SEYNDGIITVNGHSYENRKTNNTNFAILVSTHFTEPFKEPIEYGRYVARLANMLSGGV
                     VIQRLKDLQLGRRSTPERIKKSVLKPTLNGASPGDLSFVLPYRFLQDVLEIISAMDKF
                     IPGMSSPHNLLYGLEVKFYSSRVKLSEYLESDIENLFMAGDGAGVTRGLIQASTSGVI
                     VAREILHRVGGHIE"
     gene            complement(5295..>6218)
                     /locus_tag="PK171_02235"
     CDS             complement(5295..>6218)
                     /locus_tag="PK171_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT05055.1"
                     /translation="VGYLGQHHARIYSEIPDTTLVGVVDINQERAREIAKRYSTTPYY
                     DYRELFGKIDAVSIVVPTVLHRLIAGHFIEEGINILIEKPVTTTLEEARELMEMANKK
                     KVILQVGHIERFNTAVMELSKIIDRPVFIDCYRMGPYTNRNTDVGVVLDLMIHDIDIV
                     MSIVKSKVTKISSFGYPVFSRQEDIANAQILFENGCIANLTASRITRKKIRRMEITEV
                     DAFISIDYLEQELAVYKKTITAVPPILMEKPMMQKGEPLRFELEHFTRCVKNGERPLV
                     GLEEGKNALEVALKILEEIKKNQGIVNEKYL"
BASE COUNT         1697 a         1376 c         1135 g         2000 t
ORIGIN      
        1 cccaccttca tcataagttt tctctatgat tttaaacctt tttatgctta aatagtagga
       61 acaaattttt gattcaatca agcaattgga ggaaggcaat gaaaaagtac atttttgggt
      121 attttccctt agttattctt gcctatctta tcccttatac cttactgcaa aaggttcaca
      181 aattctatgg aagtttttta ttctggattg gatacacatt aatagtaata tacctcatga
      241 aaaaaatgat ggatcgctgg aaaaatgaaa attgaactta tttggatatc aattattatc
      301 tatatggctt ggggaacgct aatcgctgtt ctttctcgcc ggggaggagc aagtggagtt
      361 gtcgactact ttcttgccga ccgaaaacta aaaggataca tctcatctct ctcctatagt
      421 gctacgacat acagcgcctt tatgctggtt ggtttagccg ggctaaccta taaaggtggc
      481 gttggggctc tcggctttga actcatatac ctatccggtc ttttttgggc agttcttttt
      541 ggcccctttt attggcgagc tggaatacat tataattgtg taagtcctgc tgaattgctg
      601 agcaaccgct atcaaaataa aaaagttggc gccttatcag catggatttc tctaattttt
      661 ctgattccct attcttcgat tcaattgatg ggaatcggat acctattaga aactttgtca
      721 aatggaaaaa tccccttctt atggggtgct ctcatcgcaa ccttgattac tttggtttgg
      781 actgaaattg ctggtcttcg ttcagtagct acgaccgata gcttacaagc tgggattatg
      841 ctaatcagtt caataatctt tatttttata ttaataggga aatttttagg aggtttcgac
      901 aactttcttt atcgaattga aactaactat cctctatggt tatcagttcc ggggaatggt
      961 tattttagtt ttcaaacttt tttaggatta tctcttccct ggtttttttt cagcatatct
     1021 aatcctcaag tgtcacagcg attattcgtt ccctcttcgc tttcgaaaat gcgatctatg
     1081 ataaatggtt ttctcggtta tggttttgtt tatactctca ttaccattct aattggattt
     1141 tgcgccctcc tgctatttcc cggcctttcc aatcccgacc aggcgacacc aaccctactt
     1201 gctcaactca atattccgcc aattattgtc ctattgatga ttgtcggaat attttcggcg
     1261 gccatctcaa ccattgactc ggtgatgtta accatctcct cgatgtttgt tcgagatatt
     1321 gttcgtgctc ggagggaaat tcctgaaaag caacagttgg ttattgcccg ttggtttatt
     1381 ttaattttgg gtgtcggaat ctttctcttt gctattcagc gcttcaatct tattgctgtc
     1441 ctttccgttg cttcttcggc cgggctactg gctactgttc catcaacaat aggcggtttt
     1501 ttatggaaga aaggaagcgc atctggggct ttaattagta tgatcggagg agcctgtatt
     1561 tccctgatac ttcaaataac atcctggaaa ccttggggat ggtggccggg tgtttggacg
     1621 attattattg cgactctcct ctacatcatc atgagctatg ctaaacctgc agcaataaac
     1681 caggagtttt ttaaggtaat tcaaagccag cctaaaaaaa cctaattctc ccatcaaggg
     1741 agaagaaact ctttactccg tcaacttgag ttatgccttc ccgcgtgagg atctcatcca
     1801 ctcaatccgt cattgcgagg agtctaaccg tcttttggtt agacgacgtg gtaatctctt
     1861 aaggtcaatc attaatgttt gtactcctct ttgactcaat aggattattg taatttcccc
     1921 tcattggagg aatgaagtta acaattcccc tccaaggagg ggtgccgctt gcggcgggga
     1981 gggtgccttt aattcgtcat cctgagcagt atttttccgc gtgaggatct catttttaag
     2041 ttattttgta tttcatagat acaaaggatg ggattctcgc gtcgcaaatg acgctcctta
     2101 gaatgacaga gaagaggagn nnnnnnnnna aaaaacgagg ggaagaaaaa gattgagctt
     2161 aagagattgc cacgtcgtac cagacgctcc tcgcaattac ggattgtgtt gagggctttt
     2221 tagaggatat cctctatcaa tggaacaaaa acgactgatc catgtttttc aattttcaac
     2281 gatccttttt gttttcttcc ccgaaccaga gtttgacgaa aatcaccccc caaagggaga
     2341 acaatgattc cgccttcttt taattcctca acccaaactg ggtatatctc attagcataa
     2401 gcagttacta taattttgtc gaaaggagca tattctggaa ttccccgact tccatcacca
     2461 acaaaaaaat ggatattcct atattcaaga ctttccaaaa cctcttcggc tttttgagcc
     2521 aattcttcga tgcgttctat cgtataaacc tctttgccta attctgcgca aaccgcggtt
     2581 tgataacccg aaccggttcc aatttccaaa attcgatcgg ttggctgaat ttctaacaat
     2641 tctgacatca acgctactat ataaggttgt gaaatagttt gaccctcacc aatgactaag
     2701 ggggtatcat tgtaagcata tttccgataa aattctggaa caaaaacgtg tcgaggaatt
     2761 tttagcaggg cttgagttac tctttcatct ttaatattcc gggagatgat ttgctttcga
     2821 accatctctt cccgttcttg aattgttctg acattatcga tttccatcag tattcaacct
     2881 gaaatgattc ataatttcct gaataaggca accattcttc aaaaatatca cggattgatg
     2941 cgctcactgg tccctcacga atttcctgaa agaaatgttc aacttcggat ttctctcctt
     3001 cagctaccac ttccactcca tttggaaata aatttttaac aaatccagta acctcaaatt
     3061 gggaggctct ctttaaaaca aaataacgaa aaccaacccc ctgaactcgg cccttaaata
     3121 ccaaccttcc ctgaatgaac ttcatttcaa ttattcttct ccgacaatac ttctaccagt
     3181 tcgtttttta ctaaccaatc ggtcaggtta ggtcgaaggg gggtaatcga cacttccgaa
     3241 ttcactaccg accaataatc cgagctactt tggaattcaa tctggcgttt ttcttcaata
     3301 aaaacatatt cctggaattc ttcattctga atggtttttt ggactttagt aaaataaaac
     3361 cgatcggcaa gctcggtaat cttccaaccc ttcagttcat ccgaatgagt cacatcaggt
     3421 atattcacat tgagaactaa atttttttgt cgagaaagtg tttcttcaaa atggagaaat
     3481 atatcaagag cagtttcagc agcaacctta taattcaatt tttcttgtat tgatagtgaa
     3541 accgccatgg ctggtattcc cgacatggca gcttccatgg ctgccccaac cgttcccgaa
     3601 taaaatacat caaaacctat gttgggccct cggttgatac cggatatgac aaaatggacc
     3661 ggagtttggg aaaatagatt gagacctaag ataaccgtat cggccggtgt cccatcaact
     3721 gccaagccac ttactccatc tacttgaacg tccttcactc ttaatgtctg tcgtgctgta
     3781 atactatgac ttgaacaact tcgctcttca agaggagcaa ctaaaataat attggctatt
     3841 ggcttaaaaa ctcgaactaa agccataatc ccatcacttt gataaccatc atcattggta
     3901 ataaggatgt ttcttttcat tcaatatgac ctccaacccg gtgtagaatt tctctggcta
     3961 cgatcacacc cgatgtggac gcctgaatca aacctcgggt aacaccggca ccatctccag
     4021 ccatgaataa attctcgata tcgctttcta aatattcgct gagtttcacc cgagatgaat
     4081 agaactttac ttctaatcca tagagaagat tatgcggaga actcattcct ggaataaact
     4141 tatccatagc cgaaataatc tctaatacat cttgcaaaaa ccgataaggg agaacaaaac
     4201 tcaaatcccc cggactagca ccgttgagtg taggctttaa aactgatttt tttattcgtt
     4261 ctggagtaga ccttcttccc aattgtaaat ctttaagcct ttgtataaca acaccgccac
     4321 tgagcatatt tgctaagcga gcaacatagc ggccatattc gattggttct ttgaagggtt
     4381 cggtaaaatg agtacttacc aggatggcaa aattggtatt gttggttttt cgattttcat
     4441 aactatggcc attaacggtt ataatcccat cgttatattc actgaccacc tcaccatgag
     4501 ggcacataca aaaagttctc actcgatcgt caaaggtatg ggaataatag atgagtttta
     4561 attcgtacaa aactttggtg tattcttcca taattgaagc cggtacttcc atcctaacgc
     4621 ccaaatctac gggattattg actaaatcaa gctttaattt cagtgcttct gtccttagcc
     4681 aatcagctcc aacccgacca ggtgcaacga tcaagtagtg gcttttaatc acatcgccat
     4741 ttttcagctg aaccccaact gcttgatggt ttttggttaa tatttcggca gcttcagtac
     4801 gagtgaggat ggtcaccttc tctttcaaat gatttttgat acgagttaat attcctcgac
     4861 agctgtcagt tcccaaatgt ctcactcgag atgggagcaa tatcagttca gccatttccg
     4921 cttttttccg gagttcttcc accttttctc catcatctcc ataaacccgt ggggttgctc
     4981 catagcgaag gtagatatta tcaacgtact gaagaagctg ttccaaatca ttcggtttta
     5041 aataagaagt caactgacct ccaacatcgt gggaaaaagt cagcttccca tcactgaatg
     5101 ctcctgctcc tccccagcca cagagcaatg aacatggctg acatttttgg cagatatttt
     5161 tcttttcccg ggctgggcat cttcgttctt caatatctcc gcctcgatca attattagaa
     5221 tatcaagatc ggttttatcg gcaagttcta atgcggcaaa aattcccgct ggtcctcctc
     5281 cgattataat aacatcatag gtatttttca ttcacaatcc cctgattctt ttttatttct
     5341 tcaagtattt ttagtgctac ttcgagcgca tttttccctt cctctaaacc aaccagtggt
     5401 ctttctccgt tttttacaca acgggtaaag tgttccagtt caaatcgaag cggctcccct
     5461 ttttgcatca tcggtttctc catgagaata gggggaactg ccgtgattgt ctttttgtaa
     5521 accgctaact cttgctccaa ataatcgatg gaaataaaag catcgacttc ggtgatttcc
     5581 atacggcgaa tttttttcct ggtgattcgg ctagccgtaa gattggcaat gcaaccattt
     5641 tcaaaaagta tctgtgcatt agcaatatct tcttgacgag aaaatactgg atatccaaaa
     5701 gaactgattt tagttacttt gcttttgaca atgctcatta caatatcaat atcatgtatc
     5761 atcaaatcaa gaacaactcc cacatcggta ttgcggttgg tatagggtcc catacgataa
     5821 caatcgataa atactgggcg atcaattatt tttgaaagtt ccatgactgc tgtgttaaac
     5881 cgctcaatgt gacctacctg gagaattact ttctttttat ttgccatctc catcaattct
     5941 cgagcttctt cgagagttgt tgtaaccggt ttttctatga gtatattaat cccttcttcg
     6001 ataaaatgtc cggcaatcaa acgatgaagc acggttggga ctacaatgct cacggcatca
     6061 atttttccaa acaactcacg ataatcgtaa tagggggtcg tcgagtatct cttggcaatt
     6121 tctcttgctc gttcctgatt tatatcgact acacccacca gagtagtatc tggtatttca
     6181 gaataaattc gggcatgatg ctgtccaaga taaccaac
//
LOCUS       DAOSOL010000054         6180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_12791_length_6180_cov_4.137959, whole genome shotgun sequence.
ACCESSION   DAOSOL010000054 DAOSOL010000000
VERSION     DAOSOL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12791_length_6180_cov_4.137959"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1369)
                     /locus_tag="PK171_02240"
     CDS             complement(137..1369)
                     /locus_tag="PK171_02240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT05056.1"
                     /translation="MSFYEDLFNQLLIWKKIDTHEHFLPQNTYQDHPDILLAILRESY
                     LPWIVYGARDHNTITVTREGLLQGMKKIPASAFLRYIIEAFQYMYGFSDDEINQDNWE
                     QLSQLIRESYSKKNMLDYWIFDKFNVEKVVHDRYWKLGEFDIDQSHYFAVFRIDPLFC
                     GYSKEKLNHDRMNPHIEAAKQGIHIETFDEYLAYIDHLFKKAVAEGIGTMKCAVAYDR
                     TLRFEKVKKEQAEKAFYKKDGTELSSEVRDFQDYVFYYCLQKAADHHLPVQIHTGPGK
                     AFETAASHLGNIFEEFHDLKISLFHGSFPWVGEPGAMSLFYPNLYLDLVWLPVMSPSY
                     AVMALSEWIETAGGARIMMGGDSWNAEGAVGSLLYNLKTIARVLAEKVEKKYLSRSSA
                     EQIGKMILYDNPKEFLSR"
     gene            complement(1382..2062)
                     /gene="rpe"
                     /locus_tag="PK171_02245"
     CDS             complement(1382..2062)
                     /gene="rpe"
                     /locus_tag="PK171_02245"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT05057.1"
                     /translation="MNEQMIKISASLDCANYLDLLSDIRKLEKGGVNMLHLDIMDGHF
                     VPNYALGTNLLRKLRPQTKLLFDVHFMTSNPEVSIPIFADLGADIITFHVETTSRLHQ
                     MVSSIKNLGKKVGLALNPSTPPDILEYILPYIDMVLVMTVDPGFVGQKFVPEVVKKVQ
                     TIKNLIESHQLNIDIAVDGGIGETTVPLLKKAGANVFIAGTTSIFSGKEEIEAASRKF
                     RELCEKTY"
     gene            complement(2124..3173)
                     /locus_tag="PK171_02250"
     CDS             complement(2124..3173)
                     /locus_tag="PK171_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HOT05058.1"
                     /translation="MDTMKALVLLKKGDIQFLSVPKPKPARGEALIRVKACGICGSDI
                     PRIYGGIAYYYPSIPGHEFAGEVEEVGDPSLDKNWVGKRVTVFPLIPCYNCRLCQIGL
                     YEMCENYGYIGSRRDGAFAEYVTVPVANCMDLPGKVSYESGAFSEPAAVTLHALRRSS
                     IPFGKTVAVFGLGPIGVLFGIWAKISGARRLIGFDIDQKKVDFARENGFNEAMIPEPE
                     KLEKAVENYTEAGGFDLTCEASGSNRALVQSIKITHKFGEVMLLGNQERDVTLTLKEM
                     SLILRKQLTLHGTWNSRFTHIGSDWKAVLEFENSGQANFKPFISHRLTLEEAPEFIQL
                     MRDKKVFYNKVLIIP"
     gene            complement(3176..4213)
                     /locus_tag="PK171_02255"
     CDS             complement(3176..4213)
                     /locus_tag="PK171_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT05059.1"
                     /translation="MKAAVLEGIDQLVVKEVPDYKVGLGEVLVRVKTCAVCGSDLRIM
                     HSGNPRVKYPQIVGHEIAGEVVEVGAGVERLGVGDRVAVGADVPCGKCYWCQNGMGTN
                     CAINYAVGYQFPGGFAEYILLNELTVNQQVVNLIPDTLSFDEASLAEPLACAINGLEM
                     SQLGVGDTLCIIGAGPIGCMMIELGRHMGATNIIVIQRSQKRLDMAKKYGADHYFLSN
                     DPDIEAKVKGVTKGEGPDVIMITCASPDAQEQSLLLARHRARINFFGGLASTARKLNI
                     SSNLIHYKELNVLGSHGSLPRHHKKAIQIIDKRMVHTEQYITHRFPLDQIHEAFHAAE
                     SREGLKVVVNP"
     gene            complement(4666..5103)
                     /locus_tag="PK171_02260"
     CDS             complement(4666..5103)
                     /locus_tag="PK171_02260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT05060.1"
                     /translation="MVTTFKATGHWDGCLRIKSIVRDFSLAYDEPPSLGGEDTAPNPV
                     EAILSSLVGCLGIVACVVANEKKIPFEGIDISVEGDLDPRGFMGQDKNVRPGMQSIRY
                     NIKVKGNLSDTQLKEFLEEIEKRCPVSDTLKNGTSVTGRIGKV"
     gene            complement(5171..5782)
                     /locus_tag="PK171_02265"
     CDS             complement(5171..5782)
                     /locus_tag="PK171_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HOT05061.1"
                     /translation="MNFINKKEMMNRLHEKTIYYFSQPGKENTQKTLELAVERALLLQ
                     LEAILVATKSGETPLRLAEVAQAFRFAGRIIAVTYHQGFFDNETLLISKENQKLLAEK
                     NIPIVMSSHALSGVSRSFREKFGGISIPEIIAESFRRISQGFKVAVEISIMAADAGMI
                     SSKQDVVAVGGQSRGADTALVLRPAHQNSFLDLKIKEVICFPS"
     gene            complement(5766..>6180)
                     /gene="phoU"
                     /locus_tag="PK171_02270"
     CDS             complement(5766..>6180)
                     /gene="phoU"
                     /locus_tag="PK171_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOT05062.1"
                     /translation="AAVMKIITDVERIGDYSIDIAKFSIRLADKPLFKPLIDVPKMAD
                     IVVKMLEETSATFIQKDLNVVQKVVEDDDKVDTLYRAVHEEVVNYIEKDPSVTRQAIW
                     ILMIARYLERIGDHITNITERIYYMETGEMIELHQ"
BASE COUNT         1857 a         1353 c         1058 g         1912 t
ORIGIN      
        1 aatctcatcc tttaaagtat ttatgaagaa agaaactaaa agatgagatc ctcacgcggg
       61 aaaacaccaa tccggatgag gaattaaaga attcatttga tggtattttc aggatagaaa
      121 aaaatacttt gaccgcttat ctgcttaaaa actccttggg attatcataa agaatcattt
      181 tcccaatttg ttcggctgac gaccggctca gatacttctt ttcaactttt tccgccaata
      241 ctcgagcaat ggttttgaga ttgtaaagaa gagatccaac tgccccctcg gcattccatg
      301 aatctcctcc catcatgatt cgagccccac cggcagtttc aatccattcc gacaaagcca
      361 taacagcata ggatggcgac atcaccggta accaaaccag gtctaaatac aggttcggat
      421 aaaagagaga catggctcct ggctcgccaa cccaggggaa actcccatgg aaaagcgata
      481 tttttaagtc gtggaattcc tcaaaaatat tccctaaatg tgaggcagct gtctcgaaag
      541 cctttcccgg accagtgtga atttggactg gtagatgatg atcagccgct ttctggaggc
      601 aataatagaa aacatagtcc tgaaaatctc ttacttcaga agagagctcg gttccatcct
      661 ttttataaaa agctttttca gcttgctctt ttttaacctt ttcaaagcga agtgtccgat
      721 cgtaggcaac cgcacatttc atggtaccga ttccctccgc aactgctttt ttaaaaagat
      781 gatcaatgta agcaagatat tcatcaaatg tctcaatgtg aattccctgc ttggcagctt
      841 cgatatgtgg gttcattcgg tcatgattta atttttcttt tgaatagcca cagaacaacg
      901 gatcaattct aaaaacggca aaataatggc tttggtcaat atcgaattca cctaatttcc
      961 aatagcgatc atgaacaacc ttttcgacat tgaacttatc aaaaatccaa tagtccagca
     1021 tatttttctt tgaatagctt tctcgaataa gttgggataa ttgttcccaa ttatcttgat
     1081 tgatttcatc atcggaaaaa ccatacatat attgaaaagc ttcaatgata taccgtaaaa
     1141 aggcgctggc cggtattttt ttcatacctt gtaaaagacc ttctcgggtt acggttatag
     1201 tattgtgatc tcgtgctcca taaacaatcc agggaagata actttcccga agaatggcca
     1261 acaaaatatc gggatgatct tgataagtat tttgagggag aaaatgctca tgggtatcga
     1321 tttttttcca gatcaggagc tgattaaaaa gatcttcata aaaagacatt tttttcacct
     1381 cttaataagt tttttcacac aattctctaa attttcggga agcagcttca atttcttctt
     1441 ttccggaaaa gatactggta gtcccggcta taaatacatt ggcaccggct ttctttagaa
     1501 gtggaactgt cgtctcacca attcctccat ctacagcaat atcaatattt aattgatggg
     1561 actcaatcag gtttttgatg gtttgaactt tttttaccac ttctggtaca aatttttgcc
     1621 caacaaatcc tggatctacc gtcataacta aaaccatatc gatataagga agaatatact
     1681 ctaaaatatc tggtggtgtt gaaggattta gggctaatcc gacctttttc cctaaatttt
     1741 ttatagaact gaccatctga tgaagacgag atgtggtttc aacatgaaaa gtaatgatat
     1801 ccgcacccaa gtcggcaaaa atgggaattg acacttcagg attactggtc atgaaatgaa
     1861 catcaaacag aagtttggtt tggggacgaa gttttcggag taaattggta cccagagcat
     1921 aatttggtac aaaatgtcca tccatgatat ccaagtggag catgttgact ccaccttttt
     1981 ctaactttcg aatatccgat aacaaatcta aataattagc acaatccaag gatgcactaa
     2041 tttttatcat ttgctcattc atgactgact ttcctccttc agtattttta gttgagtcat
     2101 taatattttt gtgaattggt ttatcaggga atgatgagaa ctttgttgta gaatactttt
     2161 ttatctctca tcagctggat aaactcagga gcttcttcca aggtaagacg atgagaaatg
     2221 aaaggtttaa aattagcttg gccagaattc tcaaattcca gaactgcctt ccaatctgat
     2281 ccaatatgag taaatcgaga attccaggtc ccatgtaacg tcagctgttt tctcaaaata
     2341 aggctcatct ctttaagggt taaggtaaca tcccgttcct ggtttcccaa gagcatgacc
     2401 tcgccaaact tgtgagttat ttttatggat tgaactaagg ctcggttgct tcccgaagct
     2461 tcacaagtga gatcgaaacc accggcttcg gtataatttt ccacggcttt ttctaatttt
     2521 tctggttcgg gaatcattgc ctcattaaaa ccattttccc gggcaaaatc aacttttttt
     2581 tgatcgatat cgaaaccgat caaacgcctg gctcctgaaa tttttgccca gattccgaaa
     2641 agtactccaa tgggacccaa cccaaaaaca gcaacagttt ttccgaatgg tatcgatgac
     2701 ctccgtagag catgcagggt tactgccgcc ggttcgctga atgcccccga ttcataagaa
     2761 acctttccgg ggagatccat acaattggct accggaacag taacatattc agcaaaagct
     2821 ccgtcacgtc gcgatccaat atacccataa ttttcacaca tttcatataa cccgatctgg
     2881 cataaacgac aattatagca gggaatcaaa ggaaaaaccg tcactctttt tccaacccaa
     2941 ttcttatcta aactgggatc gccaacttcc tcaacttcac cagcaaactc atgtccgggt
     3001 atcgagggat aataataagc aatacctcca taaattcgag gaatatcaga tccacagata
     3061 ccacaggcct ttactcgtat tagggcttct cctctcgctg gttttggttt gggaacgctt
     3121 aggaattgaa tatcaccctt tttcaacaga actaatgctt tcatggtatc catctttaag
     3181 ggttcaccac cactttcaat ccttctcttg attcagcagc atggaaagct tcatgaattt
     3241 gatccaaagg gaaccgatga gtaatgtatt gttcggtatg aaccattctt ttatcaatga
     3301 tttggatagc ttttttatga tgacgaggta ggctaccatg acttcctaat acgttaagtt
     3361 ctttataatg aattagatta ctggatatgt tgagttttct ggcggttgaa gccaaaccac
     3421 caaaaaaatt tattcgtgct cgatgtcgtg ctaaaaggag ggattgttct tgggcgtcag
     3481 gagatgcaca ggtaatcata atgacatctg gtccttcacc cttagtaact ccttttacct
     3541 tggcttctat atcagggtca ttgctgagga agtaatgatc ggcgccatat tttttagcca
     3601 tatccaaacg cttttgcgac ctttggataa caatgatatt ggttgctccc atatgtcgtc
     3661 ctaactcgat catcatgcaa ccaatcggac cagcccctat tatacaaagg gtatctccaa
     3721 ctcccaactg actcatctcc aaaccattta tagcacaggc taatggttct gccagactgg
     3781 cttcatcaaa agataaagta tcgggtatta ggttgacgac ttgttggttt acagtgagtt
     3841 cgttcaaaag gatatattct gcaaacccac caggaaattg ataaccgact gcataattga
     3901 tagcacagtt ggttcccatg ccattctggc accaatagca tttcccacaa ggaacatcag
     3961 caccgacggc tactcgatct ccaaccccta accgttcaac accagctccc acttcaacaa
     4021 cttctccagc gatttcatgt ccgactattt gaggatactt aacccgagga tttcccgaat
     4081 gcataattcg taggtcagat ccacaaactg cacaggtttt gactcgaacc agaacttctc
     4141 ctagtccaac tttataatct ggcacctctt taaccaccaa ttgatcaatt ccttctaaga
     4201 cagcagcttt catgtgattc ctccattatt tatttttaaa ttcattaacg ataataaaaa
     4261 agggggtgac agataggagc aaatggaatc tcaagtattg gtcaacaaaa ggtaaataaa
     4321 ctccctaaaa gcacaggaaa aactctcctg taatctccat caccgaatgt tacttttcat
     4381 ccttttcaga tttcacaacc ggttttaaac tcttattaac ctaatataac atataaatct
     4441 ggcgaacttt tttagtttct acgtaatatc ccaagattaa aattgtttta atcaatttgg
     4501 tttgaaaata aaaagccaga gggtacgatt ccctctggct aaaaaaacca attacaattc
     4561 gcacctccgg ctatgacata ccaattgaac tacaatttaa tttaaatcag tttttagaaa
     4621 attaaagatt ttcaatattt caaacactgt tatttcaaag gcatactata cttttccaat
     4681 tcttccagtc acacttgttc catttttcaa agtatcggat actggacatc ttttttcaat
     4741 ttcctctaaa aattctttca gctgtgtatc gcttaaattt cctttgactt tgatattata
     4801 tcgaatagac tgcattccag gtctgacatt tttatcttgt cccataaatc ctcttgggtc
     4861 aagatcgcct tcaacactaa tatcaatccc ttcaaaaggt atctttttct cattagccac
     4921 tacacaagca acaataccta aacatccaac taaagacgag agtattgctt caactggatt
     4981 gggagcggta tcttcgccac caagagaagg cggctcatca taggcaagtg aaaaatcacg
     5041 cactattgat tttattctca aacatccatc ccaatgtcca gtcgctttaa aagtcgttac
     5101 catgtgttat tcctcctttt gcataagaat tccctaattc cccaacctta ttcgttaacc
     5161 aatgaagttt ttaagaagga aaacagataa cttcctttat ttttaaatct agaaaagaat
     5221 tttgatgtgc cggtcttaat accaaagcag tatctgctcc ccggctttgc ccaccaactg
     5281 ctactacatc ttgttttgat gaaatcatac cagcatcagc agccatgatg gagatttcaa
     5341 ctgcgacttt gaaaccttgg gatattctcc ggaagctttc ggcaataatt tctggaatgc
     5401 taatcccacc gaatttctct cgaaaagacc gactcactcc actcagggcg tgagatgaca
     5461 tcactatggg aatatttttc tcggcaagca gcttctgatt ctcttttgaa atcaataagg
     5521 tctcattatc aaaaaatccc tgatgatagg tcacggcaat tattcttcct gcaaagcgga
     5581 aagcctgggc tacctctgcc aaacgcaatg gcgtttcacc gctttttgtc gcaaccagta
     5641 ttgcttctaa ttgaagcaat aaagcccttt caacggctaa ttctaacgtt ttttgagtat
     5701 tttctttacc aggctgggaa aaataatata tagtcttttc gtgtaacctg ttcatcatct
     5761 cttttttatt gatgaagttc aatcatctct ccggtttcca tataataaat ccgctctgta
     5821 atattagtga tatgatcccc aattctttct aaataacggg cgatcataag aatccaaata
     5881 gcctgcctgg taactgaagg atctttttca atataattga caacttcctc atggactgct
     5941 cgatacagag tatcaacttt atcgtcatcc tcgacaactt tttgtacaac attcaaatct
     6001 ttttgaataa aagttgccga ggtttcttct aacattttta ctacgatatc agccattttt
     6061 ggaacatcaa tgagtggttt aaaaagtggt ttatcggcaa gacgaatgct aaatttggcg
     6121 atatcaattg aataatcacc tattctttct acgtcggtaa tgatcttcat tacagcggct
//
LOCUS       DAOSOL010000055         6094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_13049_length_6094_cov_4.370426, whole genome shotgun sequence.
ACCESSION   DAOSOL010000055 DAOSOL010000000
VERSION     DAOSOL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13049_length_6094_cov_4.370426"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..2836
                     /gene="secA"
                     /locus_tag="PK171_02275"
     CDS             164..2836
                     /gene="secA"
                     /locus_tag="PK171_02275"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT05063.1"
                     /translation="MFGLIKEYFGKISQDRFLKDVTERFVDPVNSYEKEISLLNDKEL
                     ADKTLYFKNRLENGESLDDILVEAFAVVREAARRVTGMRPFDVQIIGGVVLHEGRIAE
                     MQTGEGKTLVATMPAYLNALTGKGVHIVTVNDYLARRDRYWMGPIFEFLGLTVGLIQH
                     ASTFEERKIAYRSDITYGTNTEYGFDYLRDNMAHRREDIVQGELNYAIIDEVDSILID
                     EARTPLIISGPAEDSTEIYYKIDRVARKLSHSVDFDFEEKTRSIWLTEEGISKVEKLL
                     HVDNLYDTAGKDSIDQRVRQSLRAHHLYKKDVDYVVRNGEVIIVDEFTGRLMEGRRYS
                     DGLHQAIEAKENVQVANENQTLASVTYQNYFRMYNKICGMTGTAKTEEDEFIYTYGMS
                     VVVIPTNKPLKRTNYPDVIYRTEKEKFEAVVEEIEKWHQEGRPILVGTVSIEKSEKLS
                     RMLEKKKIPHQVLNAKNHEREAAIIAQAGRKGAVTISTNMAGRGTDIILGGNPEYLAR
                     EELTNQGHNLEFDLNPDQQNHLQQLVQKSQKIAEEEHALVVEKGGLHVVGTERHESRR
                     IDNQLRGRAGRQGDPGSSRFFLSMEDDLLRLFGSERISGIMDKFGVEEGVPIEHALVT
                     RAIEGAQKKVEGYHFNIRKTLLQYDDVMNKQREVIYNQRRTLLAENNLRPLIFGMLND
                     VVHECVSAYAAEKTYPEEWDWDGFDRRLFDLFGFGHGVPMKQRPQAKPEDLERLIITR
                     YKEIYEQKVSEVNDQVFREVERFVGLRVVDGYWKEQLHNMDHLREGIGLRAVGQKDPF
                     VEYQIEAFDMFHEMIAQIREDIVRYLLRIRLVPEGSKPPAPSRRPVQSSPVRTDQVQP
                     LENQKPDVAKKGKIGRNDPCPCGSGKKYKYCCGK"
     gene            3117..4133
                     /gene="prfB"
                     /locus_tag="PK171_02280"
     CDS             3117..4133
                     /gene="prfB"
                     /locus_tag="PK171_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT05064.1"
                     /translation="MQLKVQQIEKKMNSPDFWNNTQNERELQDYKKYKSLIEEYEELK
                     KEYQLIVELQEIITPDDDEYSDLEKDIHNLSGKVEDLDIRLLFRNQEDQSNAIITIHS
                     GAGGTESQDWVQMLFRMYSRFCERKKFDVKVTDVLEGEEAGLKHITFLVQGQYAYGYL
                     KSESGVHRLVRISPFDANRRRHTTFALVDVIPEVSEEINIDINPQDLRIETFRAGGRG
                     GQHVNTTDSAVRIVHLSSGLIVQCQNERSQHQNKAVAMRILRARLYELMKKEQEEKIL
                     EIKGEQKEIAWGNQIRSYVFQPYSMIKDHRTDYEVGSVQRVMDGEIDDFIDAFLRMDW
                     QGQV"
     gene            4145..5038
                     /locus_tag="PK171_02285"
     CDS             4145..5038
                     /locus_tag="PK171_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05065.1"
                     /translation="MKVYALVGPTGTGKSYRAPHIAQRYGIDCIIDDGLLISQGSIIA
                     GKSSKAEVSKIKAAKIAVFYFTDHRQQIIEALKKIQPPKIMILGISLAMVEKICERLQ
                     LPYPSEIVMIEDIASPEEIENALQARIGEGKHTIPVPLVELKRNLWGNIVDTIPVKVW
                     GWFYSSREKTVVRPPFSYLGKLVVSESALRSIILVLLTNLPWIKKINDIFLHKVESHV
                     NVRVGCVFKNYYQLVQICAFIQKYLKEKVEHLSGVELIEVNIDIDQIAIIKNGEIVEK
                     RTTKVKKSSRSERTRISSSRK"
     gene            5548..>6094
                     /locus_tag="PK171_02290"
     CDS             5548..>6094
                     /locus_tag="PK171_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT05066.1"
                     /translation="MSRKKMEELINSLVNKKEKILLGGGVAAIDKQHQSGKLTARERI
                     FLLLDENSFEETDLFIEHRSTRFGMDEKKPPADGVVTGYGTIDGRPVFVYSQDFTVMG
                     GSLGEMHAQKICKIMDMALKVGAPIIAINDSGGARIQEGIDSLSGYGQIFYRNTLNSG
                     IVPQIAIIAGPCAGGAVYSPAL"
BASE COUNT         2051 a          990 c         1341 g         1712 t
ORIGIN      
        1 accaaaaagt cacagggcaa actatcctta aagaatgaac tccagaaggt ctaaaggggt
       61 atgatataat gatttcgcct ttgtatgatt atcataggat ctgaaagagg gttttagtct
      121 tggccaattt acgaccttta taaaattggc ggggaggcat tcaatgttcg gattaatcaa
      181 agaatatttt ggaaaaattt ctcaagatcg attcctgaaa gatgttacag aacgttttgt
      241 tgatccagtt aattcttatg aaaaggaaat atcgcttctc aacgataaag agctcgccga
      301 caaaaccctt tattttaaaa atcgtttaga aaacggagaa agtttagatg atatcttggt
      361 tgaagctttt gcggtggtga gagaagcagc ccgtcgagta accggcatga ggccatttga
      421 tgttcaaatt atcgggggag ttgtattgca cgaaggaaga atcgctgaga tgcaaactgg
      481 tgagggcaag acattagttg caactatgcc agcctatttg aatgccctaa ctggtaaggg
      541 agttcatatc gtaacggtaa atgattattt agctcgtcgc gatcgatact ggatggggcc
      601 aattttcgaa tttcttggtt tgacggttgg cttgattcaa catgcttcaa cttttgagga
      661 gagaaaaata gcttatcgaa gtgacattac ctatggaacc aatactgaat atggattcga
      721 ttatctccgg gataacatgg cacatcgccg ggaagatata gtgcaagggg aactcaatta
      781 tgcaatcatc gatgaagtag atagcatttt aattgatgaa gcccgaacgc ctctcatcat
      841 ttcagggcca gcagaagatt cgactgaaat atattataaa attgatcggg tggcgcgcaa
      901 gctctctcat tcggttgact ttgactttga agaaaaaact cgatccattt ggctgaccga
      961 agaaggaatt agcaaagtag aaaaactcct tcatgttgac aacctctatg atactgcggg
     1021 taaagattca attgatcagc gagttcgcca gtctttaagg gctcatcatc tatataaaaa
     1081 agatgtagat tatgttgtga gaaacggtga agtgattatc gttgatgaat ttaccggacg
     1141 gcttatggaa ggtcgacgtt atagcgatgg cctccatcag gccatagaag caaaagaaaa
     1201 tgttcaagtg gcaaatgaaa accaaactct cgcctcggtg acctaccaaa attattttcg
     1261 tatgtataac aaaatctgtg ggatgactgg aacggcaaaa accgaggaag atgagtttat
     1321 ctatacttac ggtatgtcag ttgttgttat acctaccaat aagcccctta aacggaccaa
     1381 ttaccctgat gtaatatatc gtactgaaaa agaaaagttt gaagcagtgg ttgaagagat
     1441 agaaaagtgg catcaagaag ggcgtccgat attggtcgga accgtatcaa tagaaaaatc
     1501 tgaaaaatta agtcgaatgt tggaaaaaaa gaaaattcct catcaggtat taaatgcgaa
     1561 aaatcatgaa agagaagcag ctattattgc tcaagctgga agaaaaggag cagtaaccat
     1621 ttcaaccaac atggcaggaa gaggaactga tattatttta ggtggtaatc ctgaatacct
     1681 tgctcgagaa gagttaacaa accagggaca taatctggaa tttgacctca atcctgacca
     1741 acagaatcat ttacaacaac ttgttcaaaa atcccagaaa attgccgagg aagaacatgc
     1801 tttagtggtt gaaaaaggag ggttacatgt tgttggaacc gaacgtcatg agagtcggcg
     1861 gattgacaat caattacgag gacgagcagg tcgtcaagga gacccaggtt cgtcacgttt
     1921 ttttctttcc atggaagacg atctattgag actttttggt tcagaaagaa tatccggaat
     1981 tatggataaa tttggtgttg aagaaggagt tcccattgaa catgctttag tcactcgggc
     2041 gattgagggg gctcaaaaga aagttgaggg gtatcatttt aatattcgaa aaactcttct
     2101 tcaatatgat gatgtcatga ataaacaaag agaggtcatc tataatcaaa ggcgaactct
     2161 tttggctgaa aacaatttac gtccattaat ttttggtatg ttgaatgatg tggttcatga
     2221 atgtgtgagt gcctatgctg ctgaaaaaac ctatcccgag gaatgggatt gggatggttt
     2281 tgatcgccga ctatttgacc tttttggttt tggtcatgga gttcctatga aacaacgacc
     2341 ccaagctaaa ccagaagatt tagaaagatt gataattact cgctataaag aaatttatga
     2401 gcaaaaagta agcgaggtaa acgatcaagt atttcgtgaa gtggaaagat ttgttggttt
     2461 acgggtagtt gatggatact ggaaagaaca attacataat atggaccatc ttcgagaggg
     2521 tattgggctt cgggcggttg gtcaaaaaga cccatttgtt gaatatcaga tcgaagcttt
     2581 tgatatgttt catgaaatga ttgcacaaat ccgtgaggat atcgttagat atcttttacg
     2641 gattcggctc gtccccgagg ggagtaaacc accggcacct tcccgccgtc cagttcagtc
     2701 ttcaccggtt cgaaccgatc aagttcaacc gttggaaaac caaaaaccag acgttgcaaa
     2761 aaaaggaaaa attggtcgta atgatccctg tccgtgtggg agtgggaaaa agtataagta
     2821 ttgttgtgga aaataaactt ggttttttaa aataattttg atacaattct tgcttattct
     2881 gtttaataaa tagaatgatg ggtgaatagc gaacaggaat cagaagacca cagtcttatt
     2941 aggtatatat ttaattaatg ggaattaatg aatgaatctc attatatctc aaaaagaaaa
     3001 ttaggattcg atagttttta ggaggttata agtaatggcg attgatattt ctcttgaatt
     3061 cagtaccatt cgacacaagc ttagtgaaat aggagaatat ctttgaagaa gaacaaatac
     3121 aattaaaagt tcaacaaatt gagaaaaaaa tgaattcccc cgatttttgg aacaacaccc
     3181 aaaatgagag ggaattgcag gattataaaa aatataaaag tttaattgaa gaatacgaag
     3241 aactgaaaaa ggaatatcaa ctgatagttg agctccaaga aataattact cctgatgatg
     3301 atgagtattc tgatcttgaa aaagatatcc ataacctttc ggggaaagtt gaagacctgg
     3361 atatccgttt gttattccgt aaccaggaag accaaagcaa tgcaatcata actattcatt
     3421 cgggagccgg tggaaccgag tctcaagatt gggtccaaat gctttttcga atgtatagcc
     3481 gattctgtga aagaaaaaaa tttgatgtta aagtaaccga tgttttagag ggagaagaag
     3541 ccggcttaaa acacattacc tttttagtcc aaggtcaata tgcttatggt tacctgaaat
     3601 cagaaagcgg agttcatcga ctggtgagaa tttctccctt tgatgccaat cgaaggcgtc
     3661 atacgacttt tgccttggtg gatgttattc ccgaggtgag tgaagaaatc aacattgaca
     3721 ttaatccaca agatttacga attgaaactt tccgagctgg tggtcgtggc gggcagcatg
     3781 tgaatacaac cgattcggcc gtgaggatag tccatctttc cagcggtttg attgtgcaat
     3841 gtcaaaatga acgttcacag catcagaaca aagcagttgc catgagaata ttaagagcca
     3901 ggctttatga actaatgaaa aaagagcaag aagaaaaaat cttagagatt aaaggcgagc
     3961 aaaaggaaat tgcctggggg aatcaaatcc ggagttatgt ctttcaaccc tatagtatga
     4021 ttaaagacca tcggactgac tacgaagttg gcagtgtaca aagagttatg gatggagaaa
     4081 ttgatgattt tatcgatgct tttcttcgaa tggattggca aggacaggtt taggtataaa
     4141 tatcatgaaa gtttacgctt tggtaggtcc aaccggaaca ggcaaaagtt atcgagctcc
     4201 tcatatagct cagagatatg ggattgactg cattattgac gatggactat taatttccca
     4261 ggggagcatt atcgccggaa aatcttctaa agcagaagta agcaaaatta aagcagcaaa
     4321 aatcgcggtt ttttatttta ctgatcatcg tcaacaaatt attgaagcat taaaaaaaat
     4381 tcaaccccct aaaataatga ttttaggtat ttctctagca atggttgaaa aaatatgtga
     4441 gcgtttgcag cttccttatc catctgaaat tgtgatgatc gaagacattg ctagtccaga
     4501 agaaattgaa aatgctcttc aagctcggat tggagaaggg aaacatacta ttccagttcc
     4561 tttggttgaa ctaaaaagaa atttatgggg aaatattgtt gatactattc cagtgaaagt
     4621 ttggggttgg ttttattctt ccagagaaaa gactgtggta cggccacctt tcagttattt
     4681 aggcaagttg gttgtttcag aaagcgcttt acgatctatc attttagttc ttttaaccaa
     4741 ccttccttgg attaaaaaaa tcaatgatat ttttttacat aaagttgagt ctcatgttaa
     4801 tgttcgagta ggttgtgttt ttaaaaatta ttatcaatta gtccaaatct gtgcttttat
     4861 tcagaagtat ttaaaagaaa aagtagaaca cctcagcgga gttgagctga tcgaagtcaa
     4921 tatcgacatt gatcaaatcg ccataattaa aaatggagaa attgttgaga aacggacaac
     4981 taaggttaaa aaatcatcac gatccgagag gacaagaatt tcttcttctc gaaaatgaaa
     5041 ccgattcatt ttaaaaaata atgataaatt ttcatgccac tttgatagca ccaaatgagg
     5101 atgaaaaata cctacctaaa acgtctttac gaggagtcca aacgttcttt ggttggccaa
     5161 cgtggcaatc tcatcagccc actccgtcat tctgaggagc gtattttgcg acgtgagaat
     5221 ctcatcctta agaatatttt aatataaaaa gaaataaaaa atgagatcct cacggcttct
     5281 aaaagcgaag cctcaggacg atgtatcaaa atagttcctt ctcccttgaa gggagaagga
     5341 caggatgagg gtgaagccca agattcaagt ctaggtttgg tgttttaaaa ttaaacttga
     5401 gggaaaaatt caaacatccc cctcacctta atcctctccc accaggagag aggaaaaaag
     5461 aaaaatgaaa gaaggtgagg atgagagtta agatagaatt ggttttaact ttaatggatt
     5521 aaataaaaaa ctggtagggt taaatttatg tccagaaaaa aaatggaaga gctcattaac
     5581 tctttagtaa ataaaaaaga aaagattctt ttgggtggag gagtggctgc aattgataaa
     5641 cagcaccaaa gtggaaaact aactgcccga gaaagaattt ttctcttgct tgacgaaaat
     5701 agctttgaag aaaccgatct ttttattgag catcgctcaa cccgttttgg aatggatgaa
     5761 aaaaaaccac cggctgatgg agtcgtcact ggttatggaa ctatcgatgg aaggccggta
     5821 tttgtatact cccaagattt taccgttatg ggtgggtctt taggggaaat gcatgcacaa
     5881 aaaatatgta aaattatgga catggcatta aaagtaggtg cccccattat agctattaat
     5941 gattcaggtg gtgctcgaat tcaagagggc atagattccc tatctggtta tggtcaaata
     6001 ttctatagaa atactttaaa ttcaggtatc gttccccaga tagcaataat agctggtcct
     6061 tgtgccggag gagcagtata ttctccagct ctta
//
LOCUS       DAOSOL010000056         6092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_13059_length_6092_cov_3.765281, whole genome shotgun sequence.
ACCESSION   DAOSOL010000056 DAOSOL010000000
VERSION     DAOSOL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13059_length_6092_cov_3.765281"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1553)
                     /locus_tag="PK171_02295"
     CDS             complement(231..1553)
                     /locus_tag="PK171_02295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT05067.1"
                     /translation="MKRKALKRGFNPIINQKNYRNELFNSFNHNNFNSDSIHPEDQIT
                     EENQKGFNNKIEKNEISLKENEDNGIQPMDNQVKFYFYKDEEVFQDFTIKSQKYFSNN
                     IVLRDPLTGLLNRVLFEEETKRLDTERQLPISIIMGDVNGLKLVNDIYGHDYGDRFLV
                     DVAAILRDSCRKEDIIARWGGDEFIILLPKTPQETAQAVVNRISENCQKRKTETIPIT
                     ISLGIGTKNLPSDQIKEALKQAENDMYHKKTIENQLIRDRLFTSLKLKYWEVKKLFQK
                     EGNTENLLYHHQLGERLLLDQQAINELLLLAALHDVGKITIPLHLLLKKEPLTLSEKL
                     TLKRIPEISHRILQYFPNTFSIADAALSYCEWWDGSGYPRGLAKENIPMISRISSVVN
                     SYDLMIQPRPYKNPLAPKEALTEIHNQSGKQFDPEVVKSFLKMMKEKA"
     gene            complement(1740..1814)
                     /locus_tag="PK171_02300"
     tRNA            complement(1740..1814)
                     /locus_tag="PK171_02300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:Glu,seq:ctc)
     gene            complement(1819..1892)
                     /locus_tag="PK171_02305"
     tRNA            complement(1819..1892)
                     /locus_tag="PK171_02305"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1858..1860),aa:Gln,seq:ctg)
     gene            complement(2014..3501)
                     /gene="gltX"
                     /locus_tag="PK171_02310"
     CDS             complement(2014..3501)
                     /gene="gltX"
                     /locus_tag="PK171_02310"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT05068.1"
                     /translation="MEKTVRVRFAPSPTGFLHLGGARTSLFNWAYARKTGGVFVLRIE
                     DTDVARSTDESVEVILDSLKWLGFNWDEGPGVDGPYKPYYQSMRLFFYQEQIQKLLQT
                     GKAYRCFCSAEELAERREKTMAEGKNWRYDRKCLQLSPAEVETRLSQGASSVIRFFIP
                     EGETSFIDMLRGDVIFNNVELDDFVLLKSDGMPTYNFACVVDDAYMKITHVIRGDDHI
                     SNTPRQVLLYKALGFPVPQYAHIPMILGKDKTRLSKRHGSPSVTYYRDKGYLPDAMVN
                     YLARLSWASGEEEKEIFTRQEIIERFSLDQVSKHSAVFDLDKLNWMNSVYIREADQST
                     LVQILVEILIREQVIKPNAVTLDFTHYCEKIVAVMRERMKYVGQITEDARYFFTEDFE
                     YDWIAFDKVLMNEGAEDRLVLCREEFKELDELTVEATEDVIRNLSEKQNIKAAQFIHP
                     LRMAISGVKGGPGLFELLEILGKEKVLFRIDRTLNQIKIRKQSGN"
     gene            complement(3559..3948)
                     /locus_tag="PK171_02315"
     CDS             complement(3559..3948)
                     /locus_tag="PK171_02315"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOT05069.1"
                     /translation="MDFPDTHHCFLCGNQNPMGFKLKFKEDNGDTVSEVSIPSQYQGF
                     DGVVHGGIVATFLDEIMAQAVKKSGQKAMTGTLTVKYRKPCRTDEKIQIRGRIVEING
                     RIIKAQGEIIQDQEIVAEGEGIFVIPR"
     gene            complement(4050..5183)
                     /locus_tag="PK171_02320"
     CDS             complement(4050..5183)
                     /locus_tag="PK171_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOT05070.1"
                     /translation="MKKKKMSPSKTVFWVLITSILLCSAVLIYELQQDGVLNNKYIAL
                     AKVIPSQQDRWLSLFLFHEMNNLFPDLAVQIIENPETGKSWKVMMKDVPEYHREEISN
                     RLQAIFNVLFGFGFYGEKRTLNEKDVYFLFRDLMPWFTLEVYFQPLYQVAIVIDDLGY
                     NRTNAELFLKLPQKITYAVFPHLPLSRSLGEKFSEYGKEILIHLPMEALDNEQNNNEP
                     LILKTGDDEARVNDIIKKAIVNLPTARGLNNHKGSKATQDPKLMRRLMLVLKNSNLLF
                     LDSVTSDQSKAFDIAQEVGLLSFRRDIFIDGNTSVEYIQQKLWDAVSIAKKRGYSIAI
                     GHVKSETYQALESFFHSFNDPEVEFVFLSELVQPSKRKSQNTP"
     gene            complement(5155..>6092)
                     /locus_tag="PK171_02325"
     CDS             complement(5155..>6092)
                     /locus_tag="PK171_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT05071.1"
                     /translation="PYARYLNEEDLKIETSDRIEGEFSGLGIVIAIKDDKLTVITPYQ
                     GSPASQAGVKAGDVISQIDGESTTGMPLDEAVRRLRGDRGTKVVIQLQREGIEEPITV
                     TVVRDIIKIKSVEFELLEEGIGLIRIIEYHGRTNLEVEDAIKQLKLLEVKGLVIDLRN
                     NPGGLLSSAIICSGLFVPRDTNILFIEDRNGNRETLENPVDKIVDLPMVALINKGTAS
                     GAEIMAGIFQVILKTPLIGETTFGKGVVQQIFPLSHQGGVIFTISKYYLPDGTDINGE
                     GIQPNVVVENEEEQVDIAIQELKRIINEKEENVSE"
BASE COUNT         1789 a         1296 c         1035 g         1972 t
ORIGIN      
        1 cacgtcgata tactcgcaat caggctgtgt cacgcaattg ctctgtaaat tatttacccc
       61 tatttataat aatattggga atagatcaac aaaatatgaa aaaatactat gtcctccact
      121 ttcacattat tcttgataca agaaatgaat tgaaacacaa ctagaatgag gtttttttat
      181 agggttttca ccctcgtctg gtactccaaa agtggcacga gggttaacta ctatgctttc
      241 tccttcatca ttttaaggaa gcttttgacg acctctggat caaactgttt ccctgattgg
      301 ttatgtatct cagttaaagc ttctttaggt gccaagggat ttttataggg acgaggctga
      361 atcattaaat catagctatt caccaccgaa gaaatcctcg aaatcattgg aatattttct
      421 ttagcaagtc cccgtggata tcctgatcca tcccaccact cacaatagct taaggcagca
      481 tcggcaattg aaaaagtatt aggaaaatat tgaagaatac ggtgactaat ttcagggatt
      541 ctcttgaggg ttagtttctc tgacaaggtt aagggttctt ttttcaagag caggtgaagt
      601 ggaatggtga tttttccaac atcatggaga gcagctaaca ataaaagctc atttatggct
      661 tgttgatcaa gaagaagcct ttcacccaat tggtgatgat aaagaagatt ttccgtgttt
      721 ccttctttct gaaaaagctt ttttacttcc caatatttta attttaatga agtaaaaagt
      781 cggtccctga tcaactggtt ttcaatagtc ttcttatgat acatatcatt ctcagcttgt
      841 ttcagggctt ctttgatttg atcactagga agatttttag taccaatacc aagtgatata
      901 gtgatgggta tcgtttcggt ttttcttttt tgacaatttt ccgaaatccg gttgaccaca
      961 gcctgagcgg tttcttgtgg ggtttttggt aaaagaatta taaattcatc ccctccccaa
     1021 cgggcaatga tatcttcttt tcggcacgaa tctctgagta ttgctgctac atcaaccaaa
     1081 aacctatcac cataatcatg accgtagata tcattcacca actttaaccc atttacatca
     1141 cccattatta tgcttatcgg cagctgacgc tcagtatcga gcctttttgt ttcttcttca
     1201 aaaagtactc gattcaataa tccagtgagg ggatcgcgaa gtacaatatt attggaaaaa
     1261 tacttttggc tttttatcgt aaaatcttga aaaacttctt catccttata aaaataaaat
     1321 ttaacctggt tatccatcgg ctgaatccca ttatcttcat tttcttttaa agatatttcg
     1381 ttcttttcaa ttttgttatt aaaacctttc tggttctctt cggtaatttg atcttcagga
     1441 tgaatagaat ccgagttaaa attgttgtgg ttgaatgagt tgaatagttc gttccggtag
     1501 tttttttggt ttattatggg attaaaaccc cttttaaggg cttttctttt caattcaatc
     1561 tttccccttg taccagttaa acttctgttt caaaaaacaa tgattttttt ccgtttttct
     1621 tctctttaaa aatgatagaa aatagatgat tatataatac tattttattt gggaggtttt
     1681 ggcaattaag ttttttaaac aacgagaaaa aagtcggatc taaaatctat tagggatatt
     1741 ggtggcgcaa ccgggaattg aacccgggct tgcggattga gagcccgccg aactaaccac
     1801 tattctattg cgccacattg gctgagaggg gtggattcga accaccattc catgatccag
     1861 agtcacgtgt cctaccctta gacgacctct cagcgatcaa gcaaaaaata taataccatc
     1921 actattcagt attttcaata ataaactgac ttctccaaac cttgatgaat tcaatattta
     1981 caaaatgagg tctatcctga aaatggttcc ttctcaatta ccactttgct ttcggatttt
     2041 tatttggttt aaggtgcgat caatcctaaa cagcactttc tctttcccta aaatttccaa
     2101 gagttcaaaa agtccaggcc ctcccttcac tccagaaatt gccatacgca gagggtgaat
     2161 aaattgagcg gcttttatat tttgtttttc agataaattt ctgatcacat cttcagtggc
     2221 ttcaacagtt aattcatcaa gttctttaaa ctcttctcga cagagaacca atcgatcttc
     2281 ggcgccttca ttcatcaata ccttatcaaa agctatccaa tcatactcaa agtcttcagt
     2341 aaaaaaatac ctggcatctt cggttatttg ccccacatac ttcatacgct cccgcataac
     2401 agcaacaatt ttttcacaat aatgagtaaa atctagggtt actgcattcg gtttaatgac
     2461 ttgttcccga attaaaatct ccactaatat ttgaaccaat gttgattgat cggcttcccg
     2521 aatataaacg ctgttcatcc aattcagttt atccagatca aaaaccgccg aatgtttgct
     2581 cacttgatct aaagagaatc tttcgatgat ttcctgacgg gtaaatattt ctttctcttc
     2641 ttcccctgaa gcccagctta atcgggctaa gtaattgacc atagcatcag ggagataccc
     2701 tttatcccga taataagtaa ccgatgggga gccatgccgc ttgcttaatc gggttttatc
     2761 ttttccaagt atcatcggta tatgagcata ttgaggtacc ggaaatccta acgctttata
     2821 aagaagaacc tgacgagggg tgttggagat atggtcatct cctcttatca catgagtaat
     2881 cttcatatat gcatcgtcaa ctacgcaagc aaaattataa gtcggcatcc catccgattt
     2941 taaaagaaca aaatcatcca gttcgacatt gttgaagatc acatcacctc gaagcatgtc
     3001 aataaaagac gtttctcctt cggggatgaa aaaacgaatc accgaactcg ctccctgact
     3061 caaccgggtt tcaacttctg ctggtgatag ctgaagacat ttgcgatcgt aacgccaatt
     3121 ttttccttct gccattgttt tttctcgacg ttcagccaac tcttcagcag agcaaaaaca
     3181 ccgataggct ttcccggttt gtaataattt ttgtatctgc tcttgataaa aaaacaacct
     3241 catactttga taataaggtt tataaggacc atcaactcct gggccttcat cccaattaaa
     3301 gccaagccat tttaaactat ccagtataac ctccactgat tcatctgtgg agcgggcaac
     3361 atcggtatct tcaatacgga ggacaaacac tccaccggtt ttcctcgcat atgcccaatt
     3421 aaatagtgaa gttcgggcac cccctaaatg aagaaaccca gtagggctgg gggcaaaccg
     3481 tactctaacc gttttttcca taaaaaagaa tcctcctcat agcaactatt tcactggtaa
     3541 ctgtcaatat aattatattt atctcggaat aacaaaaatt ccttcccctt cagcaacgat
     3601 ttcttggtct tggattatct cgccctgggc tttaataatt ctaccattga tctctactat
     3661 acgacctcgt atctggattt tctcatcagt acgacagggt tttcgatact ttacagttag
     3721 ggtgccggtc atggcttttt ggccggattt ttttactgcc tgggccatta tttcatcgag
     3781 aaaggttgcc acaatacctc catgaaccac tccatcaaaa ccttgatact gggagggaat
     3841 tgacacttcg gaaaccgtat ccccattatc ttctttaaat ttcagtttaa aacccattgg
     3901 gttttgatta ccacagagaa aacaatggtg agtatcggga aaatccatta ttcggtatca
     3961 cctcgaatca gattattaaa aagaaacgac ccgctctaat aattttgcac ggggttctaa
     4021 aaaaaaatca tttcttagtg cagctttacc taaggagtgt tctggctctt cctcttgctt
     4081 ggttgaacta attctgaaag aaaaacaaac tctacttctg ggtcattgaa agaatggaag
     4141 aaagattcca aagcctgata agtttccgac ttcacatgac caatagcaat tgaatatcct
     4201 ctttttttag ctattgatac tgcatcccaa agtttttgtt gaatatattc aaccgaggta
     4261 ttcccatcaa taaaaatatc ccttcgaaaa gaaaggagtc caacctcttg agcaatatca
     4321 aaagcctttg attgatcact ggttacgcta tctaagaata aaagattgga gttttttaga
     4381 accagcataa gccttcgcat cagttttggg tcctgggtag cctttgaacc tttatgatta
     4441 ttaagtcctc gtgctgttgg tagatttaca atggcctttt ttataatatc attaacccgg
     4501 gcctcatcgt ctccggtctt caatatcaaa ggttcattat tattttgttc attatccaag
     4561 gcttccatgg gaagatgaat caatatttct ttcccatatt cactaaactt ctcccccaac
     4621 gaacggctga gtggaagatg ggggaaaact gcataggtta tcttctgggg aagttttaaa
     4681 aagagctcgg cattggttcg attgtaacct aaatcatcaa tcacaatcgc cacctgatag
     4741 agaggctgaa agtaaacctc caaggtaaac caaggcatca gatctcgaaa aagaaaatag
     4801 acatcttttt cattgagagt tctcttttca ccataaaatc caaaaccaaa aagtacatta
     4861 aaaattgcct gaagtcgatt ggatatctct tcacggtgat attctggaac atccttcatc
     4921 ataactttcc aagattttcc cgtttcagga ttttctataa tttggacagc aaggtcggga
     4981 aaaaggttat tcatttcatg gaaaaggaat aacgaaagcc aacggtcttg ctgggaagga
     5041 ataacttttg ccagagcaat atatttattg ttaagcaccc catcttgctg gagttcataa
     5101 attaatactg ctgaacaaag caagatcgat gttattaaca cccaaaatac agttttactc
     5161 ggagacattt tcttcttttt cattgataat cctttttaac tcctggatgg cgatatctac
     5221 ttgctcttct tcattttcaa ctactacatt aggttgaatt ccctcaccat taatatcggt
     5281 tccatctggt aagtaatatt ttgaaatggt aaatataact cctccctggt gagaaagggg
     5341 gaaaatctgc tggactacac cttttccaaa ggttgtttct ccaataagag gcgtttttaa
     5401 aatgacttga aaaattcctg ccattatctc tgctccgctg gcagttccct tattaattaa
     5461 agctaccata ggaagatcaa caattttgtc gaccggattt tccaacgtct cacggtttcc
     5521 gtttcgatct tcaataaaaa ggatattggt atcccgggga acaaataaac ccgaacaaat
     5581 aattgccgag gaaagaagtc cacctggatt atttcttaaa tcgattacca acccttttac
     5641 ttcgagcagt tttaattgct taatggcgtc ttcaacctca agattggttc gtccatggta
     5701 ttctatgata cgaattaaac caataccttc ttctaataat tcaaattcga cactttttat
     5761 cttaataata tctctgacca ctgtcacggt tataggttcc tctatcccct ctcgctgtaa
     5821 ttgtatgaca actttggtcc ctctatcacc ccgaagcctc cgtactgctt catctaatgg
     5881 cattcccgtg gtagattcac catcaatttg gctaatcaca tcaccagctt ttaccccagc
     5941 ttgagaagct ggtgatccct gataaggtgt tatgacggtc aatttatcgt ctttaatcgc
     6001 aatcactatt cctaagccag aaaattcacc ttcaatgcga tcgctagttt caatttttag
     6061 atcttcctca tttaagtatc gcgcataggg at
//
LOCUS       DAOSOL010000057         5968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_13443_length_5968_cov_3.748689, whole genome shotgun sequence.
ACCESSION   DAOSOL010000057 DAOSOL010000000
VERSION     DAOSOL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13443_length_5968_cov_3.748689"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..390)
                     /locus_tag="PK171_02330"
     CDS             complement(31..390)
                     /locus_tag="PK171_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOT05072.1"
                     /translation="MDSTKIQHYKRVLLNLQSRINQLLTIEQNYLREFNPLTESRDEG
                     DFSQATVDREMGLLSRDALLQISKLIESSLKKIEDGSYGICERCHQEIPEGRLKALPY
                     TPYCVSCQSIIEKKYEK"
     gene            936..1460
                     /locus_tag="PK171_02335"
     CDS             936..1460
                     /locus_tag="PK171_02335"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT05073.1"
                     /translation="MKRSICLLCLSALGIALTFIASLIRIPIPSIRWYFHLGDTVIFV
                     VSLLFGPIAGAVSGAIGSSLADLHAGLTVWIPFSLVIKGLEGFIVGWISQGREGKMDL
                     IALFVGSLLMIGGYAIATMVLFGWPVLIYEVPVDVAQCVVAIILSLFIVRNIRKRFPI
                     ISKLKGCIEWKNQI"
     gene            1442..2485
                     /gene="rlmN"
                     /locus_tag="PK171_02340"
     CDS             1442..2485
                     /gene="rlmN"
                     /locus_tag="PK171_02340"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT05074.1"
                     /translation="MEKSNIIGLLPNDLLTFLEMMSEPSYRSKQITHWLYQQKATTWD
                     EMKNLPLGFRQVLSQRFDIGQIKIETKMVSSDQSMKYLLQLADGNGIEIVVIPHRKRI
                     TICLSTQVGCPVGCSFCATGQSGFSRNLTYQEIVESAWKTQIESNSVIKNIVFMGMGE
                     PLLNYEQLSKAIQVFNHSDGFNIGSRNMTVSTIGIPDKIIKLAQEWPQVNLAVSLHAP
                     KNELRKQLIPINDVYPISQLMKTIEQYIALTHRRVTIEYSLWTDINDQREYAFQLASL
                     LRGLLVHVNLIPGNPIAECHFVPSSPARVMTFARILEDNKINVTIREPRGQDIGAACG
                     ELYGLHHKRGVVE"
     gene            2482..3498
                     /gene="aroF"
                     /locus_tag="PK171_02345"
     CDS             2482..3498
                     /gene="aroF"
                     /locus_tag="PK171_02345"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOT05075.1"
                     /translation="MIIVLRHGTSQQEVDEIVERLKKLGFGAHISKGTERTIIGAIGD
                     ERQVNLEEKIGVLPFVEKVIPILAPYKLTSREFRSEDTVIEIGKVPIGPGKFVVMAGP
                     CAVESEKQLMETAYRVKEAGATILRAGAFKPRTSPYSFQGLGEKGLKILVKAREETGM
                     PIVTEALGIEELPLVNEYADMIQIGARNMQNFRLLEAAGRLRKPILLKRGMMSTVDEL
                     LMSAEYILSQGNYNVVLCERGIRTFESATRNTLDLSCVPLVKKQSHLPIVVDPSHGTG
                     RSELVPAMSYAALAAGADGLLIEVHPNPIEALSDGPQSQDPIQFSTMMAELKKIAGLK
                     GRTI"
     gene            3500..4015
                     /locus_tag="PK171_02350"
     CDS             3500..4015
                     /locus_tag="PK171_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOT05076.1"
                     /translation="MTYSIAIIGLGLIGSSLACDLSQWSKKISITGYDIDEETKQWCL
                     QKGMVDRIAKSPQLAAKGSDLIFIATPVRVIPKIYSDIHSYLLPQAMVFDLGSTRQWV
                     FDHLIPAPNKNLYCGFHPMGGGVEGGARFAQQKLFKGRPLLVTPYRSFKDGEKNIIEE
                     ITNCLESRVFF"
     gene            4012..4356
                     /locus_tag="PK171_02355"
     CDS             4012..4356
                     /locus_tag="PK171_02355"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOT05077.1"
                     /translation="MTAPEHDRVCALVSHLPHVIANVYASQVYEKDYSFSQFAGSSFR
                     DLTRIAGSSPEVWLDIFLTNQTQILSVIDELEGGLRIIKEFIKTEDEDGLKAFLIKVK
                     KIKEQVDDYGSL"
     gene            4337..5527
                     /gene="aroC"
                     /locus_tag="PK171_02360"
     CDS             4337..5527
                     /gene="aroC"
                     /locus_tag="PK171_02360"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT05078.1"
                     /translation="MTMVHYETAGESHGTGITTIISGFPSQLPVDVDFIQNELVRRQK
                     GYGSGPRMRMEKDTVEFLGGVRWKETLGSPISVLVRNRDSVNWKIQMDPLGFPPPDYH
                     EVTIPRPGHTDLAGMVKYQFTDIRNVIERASARETVGRCVAGSFAKMFLNQLGIGVGG
                     YVESIGGIVADTEMTLEEKIKAALNSTLATFDQQSEKKMMKVIDQAREKGDSLGGTFV
                     VVAFGMIPGLGDYTSAAKRLDAQLAAGLMSVPSVKGVEIGDGFLSAQLFGSEAHDAIY
                     YDQDREPFSFFRSTNRAGGVEGGVSTGEPIVVRCAMKPIPTLQKGLPSVDIIEKSNVV
                     ARYERSDICAVPRGLVVGEAMVAWVLASVVREKFGGDSLNEVKENFRNYLKYLRDFST
                     KKIK"
BASE COUNT         1721 a         1072 c         1367 g         1808 t
ORIGIN      
        1 cgagggttgt ggtgatcgat cctcggtctt ttatttttcg tacttttttt cgatgattga
       61 ttgacagctg acgcagtagg gagtataggg caaggctttt aaacggcctt caggaatttc
      121 ctgatggcac ctctcgcaaa ttccataact tccgtcttca atttttttta aagatgattc
      181 aatcagtttg gaaatttgta aaagggcatc acgcgagaga agccccatct ctcggtctac
      241 agttgcttgg ctgaaatcac cttcatcgcg agattcggtt aaaggattga attcacgaag
      301 atagttctgc tctatggtta acaattggtt aattcttgat tgtaggttca gcaataccct
      361 tttataatgc tgaattttag tcgaatccac ctttttccct cctttcgcat gaagaaacct
      421 gcgatgaaat gttatattca attctaccat tttatcgagg aaagtacagt gaagggataa
      481 agaccgaggg ggcgaggggg cgagggggag atttctgcca acaggcagaa gaccgcgcag
      541 acaggaaaat tccaaccatc ttatccgtca ttgcgaggaa tgttctatgc gacgtggcaa
      601 tctcatttag ccaatccttc attctgatga atttaggtgt ttttgacctc aattccgtga
      661 gattcccatc tttttatctg ggcttaattt attatattcg ccaataggtt gaaataagag
      721 gaattacaaa agatcaacct caagagattg ccacgacctc aaaaaacgag gtctcgcaat
      781 gacgaataaa caatttccct ccaacggagt gatgccacac cctaacaggg tggttacagt
      841 tttacaaaat agaagaaaca ataaagatta acagttattt aaattgaaaa ccctttattg
      901 tacaatatga aataaaaaga cctggtaaga ttcttatgaa aagaagtatt tgtttactct
      961 gtttgagtgc tctggggata gctctgactt tcatagcttc actgatccgg attcccatac
     1021 cttcgattcg atggtatttt catctaggtg atacggttat ttttgttgtt tcactccttt
     1081 tcggtccgat tgctggagct gtttctggag caattggttc atctctagct gatttgcatg
     1141 ccgggctcac ggtgtggatt ccgttttcat tagtgatcaa aggattagaa ggttttattg
     1201 ttggttggat tagccaaggg cgggagggga aaatggatct cattgctctt ttcgtgggat
     1261 ctttgttgat gattggcgga tatgcgattg ctacaatggt cctttttgga tggccagtac
     1321 ttatctatga agtgccggtt gatgttgccc aatgtgttgt tgcaattatt ctctctcttt
     1381 ttatagtgag gaacattcga aagagattcc caataatatc gaaactaaaa ggatgtattg
     1441 aatggaaaaa tcaaatataa tcggtttgct tccaaacgac ttattaacct ttcttgaaat
     1501 gatgagtgaa cccagttacc gatcaaaaca aatcactcat tggctgtatc aacaaaaagc
     1561 aaccacctgg gatgaaatga aaaatctgcc tcttggtttt cgccaggtat tatcccaaag
     1621 atttgatatt ggtcagataa agattgaaac gaaaatggtt tcatccgatc aaagcatgaa
     1681 gtatttattg caactcgctg atggaaatgg aattgaaatt gtggttatcc ctcatcgcaa
     1741 acgtataacc atctgtcttt ctacccaagt gggatgtccg gttggctgtt ccttttgtgc
     1801 taccggacaa agtggcttta gccgaaacct gacctatcaa gaaattgttg agagtgcttg
     1861 gaaaacccag attgaaagta atagtgtaat taaaaacatt gtgtttatgg gaatgggaga
     1921 gcctttgttg aattacgaac aactatcgaa agcgattcag gtatttaacc attccgatgg
     1981 ttttaatatt ggttctcgaa atatgactgt gtctactatt gggataccgg ataaaattat
     2041 caaattagcc caggagtggc ctcaagttaa tttggcggta tctcttcatg ctcctaaaaa
     2101 cgaacttagg aaacagctga tacctattaa tgacgtctat cctatttctc aattaatgaa
     2161 aaccatcgag caatatattg ccttgacaca tcgtcgagta actattgaat attctttatg
     2221 gaccgatatc aatgatcaac gggagtatgc ctttcaattg gcttctcttt tacggggatt
     2281 gctagtacat gtcaatctta ttccaggtaa tcctatagct gaatgccatt tcgttccttc
     2341 ttctccggca agggtcatga cttttgcccg aatcttggaa gataataaaa taaatgtcac
     2401 cattcgagaa ccccgtggtc aggacattgg ggcagcatgt ggtgaactgt atggtttgca
     2461 tcataaaaga ggtgtggttg aatgataatc gttttacggc atggaacctc acaacaagaa
     2521 gtagatgaaa ttgttgaacg gttgaagaag ttaggttttg gagcacatat atccaaagga
     2581 accgaaagaa ccattattgg tgcaattggt gatgaacgtc aggtcaattt agaagaaaaa
     2641 attggtgttc ttccctttgt tgaaaaggta attccaattc ttgctcctta taaattgact
     2701 agtcgtgaat ttcgatcaga ggatacggta atagaaatag gaaaggttcc tatcggtcct
     2761 ggtaagtttg tagttatggc aggaccttgt gcggttgaat cggaaaaaca gcttatggaa
     2821 acggcctatc gagtaaagga agccggagca acgattttac gagccggagc ttttaagccg
     2881 cggacatctc cttatagttt tcagggattg ggagaaaaag gtttaaaaat tttagttaaa
     2941 gcccgggaag aaactggtat gcccatagta actgaagctt tgggcataga ggaacttccc
     3001 ttagttaatg aatatgccga catgattcaa attggagcaa gaaatatgca aaactttcga
     3061 ttgttggagg cggccggaag gttgcggaaa ccaatccttc taaagcgagg gatgatgagt
     3121 acggtggatg aactccttat gtcagctgaa tatattctct ctcagggaaa ttataacgtg
     3181 gttttatgcg aaagagggat taggaccttc gaatcggcta ccaggaatac tctggattta
     3241 agctgcgttc ccttggtcaa gaaacaaagt catcttccta ttgtggttga tcccagccat
     3301 ggtactggtc gttccgagtt ggttccggca atgagttatg ccgctcttgc tgccggagct
     3361 gatgggttac ttatcgaggt tcatcccaat cctatcgagg cgctttccga tggtcctcaa
     3421 tcacaggatc ctattcaatt ttcaactatg atggcagagc ttaagaaaat tgctggatta
     3481 aagggaagga ccatataata tgacctattc aattgcgatc attggtttag gcctaatagg
     3541 ttcttcatta gcatgtgacc tttcgcaatg gtcaaaaaaa atatctatca caggttatga
     3601 tatcgatgaa gaaactaaac aatggtgttt gcaaaaaggt atggttgatc gcatagctaa
     3661 atcaccccaa ctggctgcaa aaggatcaga tttgattttt attgctacgc cagttcgggt
     3721 cattccaaaa atttattcag atatccattc ttatttgtta cctcaggcga tggttttcga
     3781 tctggggagc actcggcaat gggtttttga tcacttaatt ccagctccaa ataaaaatct
     3841 atattgcggt tttcacccta tgggtggtgg ggttgaaggt ggagcacgtt ttgcccaaca
     3901 aaagctattc aaggggaggc cgcttctggt tactccctat cggtctttca aggatggaga
     3961 aaaaaatatt attgaggaga ttacaaattg cctggagagc cgggtttttt tttgaccgcc
     4021 ccagaacatg atcgtgtttg tgctttggtt agtcacctac cgcacgtaat tgccaatgtt
     4081 tatgcctcac aggtttatga aaaggattat agttttagcc aatttgccgg atcgtcattt
     4141 cgagatttaa cccgaatagc aggttcctct cccgaggttt ggttagatat tttcctcacc
     4201 aatcaaaccc agattctatc tgttattgat gaattggaag gaggtcttcg aatcatcaag
     4261 gaatttatta aaacagaaga tgaggatggt ttgaaggctt ttctaatcaa agtaaaaaaa
     4321 attaaagaac aggttgatga ctatggttca ttatgaaact gctggagagt ctcatggtac
     4381 gggaattact accataataa gtggttttcc ttctcagctt ccggttgatg ttgactttat
     4441 ccaaaatgaa ttagtccgtc gccaaaaagg gtacggatcg ggtccaagaa tgagaatgga
     4501 aaaagacact gtagaatttt taggaggagt aagatggaaa gaaaccttag ggtctcctat
     4561 ttcagtcttg gtaagaaacc gggattcagt gaattggaag atacaaatgg atcccctcgg
     4621 ttttcctcct cccgattatc atgaggtgac aataccgcga ccgggtcata ctgacctggc
     4681 gggaatggtt aaatatcaat ttaccgatat aagaaacgtt attgaacggg ctagtgctcg
     4741 agaaacggtt ggtcgttgtg ttgcaggaag ttttgccaag atgtttttaa atcaactggg
     4801 tataggggta gggggttatg tggaaagcat cggaggaatt gtcgccgata ccgaaatgac
     4861 tcttgaagaa aaaatcaaag cggctctcaa ctccacactt gcgacctttg accaacaatc
     4921 tgaaaagaaa atgatgaagg ttattgatca agctcgggaa aaaggcgatt cattgggtgg
     4981 aacttttgtc gtggtggctt ttggaatgat tcccggatta ggtgattata cctcagctgc
     5041 taaaagattg gatgcccaat tagcagcagg attgatgagc gttccttcgg tgaaaggcgt
     5101 tgaaattggg gatggctttt tatcagccca actttttggg agtgaagctc atgacgcgat
     5161 ttattatgat caagaccgag aaccattttc cttttttcgt tctaccaatc gggcaggagg
     5221 agttgagggt ggagtttcca ccggcgaacc aattgtagta agatgtgcta tgaaacccat
     5281 accaacccta caaaagggat tgccatcggt tgatattatt gaaaaatcca atgtggtggc
     5341 taggtatgaa cgatcagata tttgtgctgt tccacgtgga ttggtagtgg gtgaagcaat
     5401 ggtggcctgg gttcttgcct cagtggtccg ggaaaaattt ggtggcgata gcctgaacga
     5461 agtgaaagaa aattttagga attatttgaa atatttaagg gatttttcta caaaaaaaat
     5521 caaatagaat atctgatctt actcaattgt ttttctttgg ggaacatctt ttaaaaattg
     5581 gagttttttt ggtatagtat tttggatata atcaagccct taccaaagaa ttaaaatatg
     5641 tcattatcct gaagctttgt attatgaagc cacgaggatt ttatcttttt aagattatga
     5701 aaaaaagtaa aataaaaaag gcacaccccc cttgaatccc ccctcaatgg gggaataaat
     5761 ttaacaattc ccctcctcgg aggggtgtca caacgtgacg gggagggtgc ctttaattcg
     5821 ttatcctgag cggtgttttt ccgcgcgagg atctcatctt ttatcatttg tggagttgtg
     5881 gaaatagaaa gaatgggatt cttacatcgt ccagaaagta tatcggactt ctcagtatga
     5941 ccggttggtt caatgagatt accacgtc
//
LOCUS       DAOSOL010000058         5850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_13803_length_5850_cov_3.628473, whole genome shotgun sequence.
ACCESSION   DAOSOL010000058 DAOSOL010000000
VERSION     DAOSOL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13803_length_5850_cov_3.628473"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PK171_02365"
     CDS             <1..779
                     /locus_tag="PK171_02365"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOT05079.1"
                     /translation="ALERGAADHLAQNQQEVFKALGLEDPIVVTPSPSWSETLARILT
                     HPTIIPILLLIAFGGIFLEIMTPGFGFPGIAGIIALLLFFGGRYFAGLAGWEPLMLFV
                     AGILLLALELLVIPGFGVTGITGISALVISLYMVLRTTSILFWEVAFSQLLFYMAIMG
                     GIFLILLFFLPNNPIWKKLGLHKKDQEKEIPEEKILNYQSLVGKKGITKSMLRPAGIV
                     EIEGRRYDVVTRGEFIEPGKMITVDEVQGNRIIVQIEREV"
     gene            784..1761
                     /gene="floA"
                     /locus_tag="PK171_02370"
     CDS             784..1761
                     /gene="floA"
                     /locus_tag="PK171_02370"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOT05080.1"
                     /translation="MDITGYLIPAIIILILVIVFFSLVPLGLWISALAAGVKISILNL
                     IGMRLRRVPPASIILPFIKATKADLELDAESLEAHYLAGGNVDRVVISLIAAERAGIP
                     LSFQRTCAIDLAGRNVLEAVQMSVLPKVIETPEVAAVAKDGIELIVKARVTVRTNIDR
                     LIGGATEETIIARVGEGIVTTIGSSHSHKAVLENPDSISKTVLEKGLDAGTAFEILSI
                     DIADIDVGENIGARLQTDQAEADKRVAQAKAEERRAMAIAKEQEMLAYTQEMRAKVVE
                     AEAEVPKALAHALHEGKLGFMDYYSLQNLQADTKMRDKLGKKEDKGKHE"
     gene            1754..1954
                     /locus_tag="PK171_02375"
     CDS             1754..1954
                     /locus_tag="PK171_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05081.1"
                     /translation="MNEIEDKSGFIPYDESNASSFEDIIQENFTTFYLFHEVFSKPAC
                     YRMNPLLAFYPWKPQTLRMFHK"
     gene            complement(2050..2865)
                     /locus_tag="PK171_02380"
     CDS             complement(2050..2865)
                     /locus_tag="PK171_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05082.1"
                     /translation="MARLNGKNIFYKIIIIIVLIIALAPILWMFLSSFKSRVDIISYP
                     PKFIFTPVMDNYKRVLQMPTLMHGLRNSLIIVPISLLLGFIFGVPVGYIFARIKFKRS
                     ADLRFFILSLRFMPPVAVALPFFLIWMRLRLLDSMPAVIFTYLTIAISTMIWLTIECF
                     KQVPVECEEAAHLEGCTQFQVFSRIALPLAIPSILGMCIFIFILLWNEFFLAFVITSQ
                     RAVTMPVASAAFAVVGMEVPWGQICASVILLSIPPLILSYFFVKFLPYFFKVS"
     gene            complement(2970..3728)
                     /locus_tag="PK171_02385"
     CDS             complement(2970..3728)
                     /locus_tag="PK171_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05083.1"
                     /translation="MTRKQFFLVFMIPTFIILFFLAFYPLLNAFYYSLQNWDLRSSRP
                     IRFVGLKNYIQLFTDARFLNSLRISLLWSVITVGFSVFFGAGLLAPLINDNAKGSFRT
                     LCLFIFIIPALLPRVGAAYMWRLMYSSSIGIINYFLSFLRIGPIGFLENPSYALYSVA
                     AIDIWQWSFLIAALTIILLQDIPKEIVEAGSLDGAKRWQLYRFIIFPVIIPPFLSIFF
                     IKMMESLRSFDFIFVLTAGGPGIATETLDMYAYW"
     gene            complement(3846..5126)
                     /locus_tag="PK171_02390"
     CDS             complement(3846..5126)
                     /locus_tag="PK171_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05084.1"
                     /translation="MKKSLFILLLITITMGLLLIPGIVSAQNKVIINVLTQPRREWEL
                     LEQYLPEFEEKENIDVVFHYFAELERRSKSRLDASTKAGQYQVYYIDEANVAEFAVNN
                     WLIPIKDYYPEEYDFADFSEPLVAVLTFNDITYGAPMTFEGNVMFYRKDLFERDGIAV
                     PQTLDEYLEVVKHFHNPPELYGTAVRGLRGSGMNVWRFSPYLKMFGGQYLDENGVPVF
                     NSPEAVKAVEYYIELIKHSPSPTLSWSDAMDNFNAGKVAIVEYANLKMDYTMDPKGSV
                     VYDKVGYSKPAAGPMGAISNTAVHGLGISVAGCPTEELKVAAGKFIGWFTSKENEIRK
                     VVAGSGLTNARKSTFASPEFAAAYPAEFVEAQVEMMKIQQMCILQIPEWPEIGDYLGV
                     KLEELFTKAYAGQSYDIQAALDDAVAYAKEVLGK"
     gene            complement(5377..>5850)
                     /locus_tag="PK171_02395"
     CDS             complement(5377..>5850)
                     /locus_tag="PK171_02395"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOT05085.1"
                     /translation="IGFEYEKNYRGCAQCLISTIFKFTGKNNNSLFQSASGLSGGMAL
                     CGDGACGGYSGGIMIMGSFIGRRFEMLEVNGDKEAQSQAYQMAQRLHDKFIETYGSVI
                     CADIHKQIFGKSFCLRSKEVRKEFEEAGAHLDKCTTVVAMAASWVADILSDEGFL"
BASE COUNT         1888 a         1101 c         1194 g         1667 t
ORIGIN      
        1 ctgctttaga aagaggagct gccgatcatc tggctcaaaa ccagcaagaa gtgtttaagg
       61 cattgggatt agaagatccg attgttgtta cgccatctcc ttcctggtcg gaaactttag
      121 ctaggatttt aacccatcca actattattc caattttact ccttattgct tttgggggta
      181 ttttccttga gataatgaca cctgggtttg gctttccagg aatagccgga attattgcct
      241 tgctcctctt ctttggaggg aggtattttg ctggtctcgc cggatgggaa cctttgatgc
      301 tttttgtagc tggaattctt ttgttggctt tggaattgtt agttattccc ggttttggtg
      361 tcactggtat taccggtatt agtgctttgg tgatttccct ctatatggtt ttaaggacta
      421 ccagtattct tttctgggaa gttgctttta gccaactgtt gttctatatg gctataatgg
      481 gagggatttt cttaatattg cttttctttt tacccaataa tcccatttgg aaaaaactgg
      541 gattacataa aaaggatcag gaaaaagaaa ttcccgaaga gaaaatatta aattatcaaa
      601 gtttagtggg gaaaaaaggg ataaccaaat caatgctccg accggcgggt attgttgaaa
      661 tcgaaggaag acgatatgac gtggtgactc gtggtgaatt tattgaaccg ggcaaaatga
      721 taaccgttga tgaagtgcaa gggaaccgga tcatagtcca aatcgaaaga gaggtgtaaa
      781 tctatggata ttaccggata cttgattccg gcaatcatta tattaattct ggtgattgta
      841 tttttttcac tggttccatt gggtctttgg atatcagcct tagcggctgg agtaaagatt
      901 agcattctta acttaatcgg catgagactg aggcgtgttc ctccggctag tattatccta
      961 ccatttataa aagctactaa agctgattta gaacttgatg ccgagtcatt ggaagctcat
     1021 tatttagctg gagggaatgt tgaccgggtg gttatttctt taattgccgc cgaaagagct
     1081 ggcataccac tttcatttca aagaacctgt gctattgact tagctggcag aaatgtattg
     1141 gaagcagttc agatgagtgt tcttcctaaa gttattgaaa cacccgaggt tgccgctgtt
     1201 gctaaagacg ggatagaact cattgtaaaa gcccgggtaa ccgtgcggac caatattgat
     1261 cgtctgatcg gtggagctac tgaagagact ataatcgccc gggttggaga gggtatcgta
     1321 acaactattg gatcatctca ttctcacaaa gcagtgttag aaaatccaga ttccatctct
     1381 aaaactgttt tggaaaaagg attggatgca ggcacagctt ttgaaattct ctctatagat
     1441 attgctgata ttgatgtggg agaaaatata ggagctcgtt tgcaaaccga ccaggctgaa
     1501 gccgataaaa gagtcgctca agctaaagca gaggaaagaa gagccatggc tattgctaaa
     1561 gaacaggaga tgttagctta tacccaggaa atgagagcga aagtggttga agctgaagct
     1621 gaagtcccca aagcattggc acatgctctg catgaaggaa agttggggtt tatggattat
     1681 tattcccttc aaaatttgca agccgatact aagatgagag ataaattagg aaagaaggag
     1741 gataagggaa aacatgaatg aaattgaaga taaaagtggc tttattcctt acgatgaatc
     1801 caatgcttcc tcatttgagg atataattca ggagaatttt accacttttt atctttttca
     1861 tgaggtattt tcaaaaccag cttgctaccg gatgaatcca ctgctggctt tttatccctg
     1921 gaaaccccaa accttgagga tgtttcataa atagtcaaat ggaatcgtta tgggtgattt
     1981 ggaattgaaa taaggacttt aaaaagaccc tcatatcact ttaaatccat gaaggtcttt
     2041 ttttattgat taactaacct taaagaagta aggaaggaat ttaacaaaga aataggacaa
     2101 aatcaaaggt ggaatggaaa gaaggataac cgatgcgcaa atttgtcccc agggaacttc
     2161 cattccaacc accgcaaaag cagctgaagc aaccggcata gtcaccgcac gttgagaagt
     2221 aatgacaaag gctaggaaga attcattcca caaaagaata aagataaaaa tacacatacc
     2281 caagatgctg ggaattgcta aaggtaaagc aatacgagaa aagacttgaa attgggtaca
     2341 tccttctaag tgagcagctt cctcgcattc aacaggtacc tgcttgaaac attcaattgt
     2401 taaccagatc atggtgctaa ttgcaatggt cagatatgta aatatgactg ctggcatgct
     2461 gtccagaagc cgtaacctca tccaaatcaa gaaaaaggga agggctaccg cgactggagg
     2521 catgaatctt aaagacagga taaaaaagcg aagatcggca cttcttttaa actttatccg
     2581 agcaaatata tacccaaccg gaacaccaaa aataaatccc aaaagaagag atatggggac
     2641 tataataagg ctgttgcgga gaccgtgcat aagagttggc atttggagaa ctcttttata
     2701 gttgtccatt actggggtga agataaactt aggaggataa ctaatgatat caacccttga
     2761 cttaaacgat gacaaaaaca tccagagaat cggggctaaa gcaataatta gaacgataat
     2821 tatgataatt ttatagaaaa tattctttcc atttaatcgt gccatctgat taatcccctc
     2881 catacgatag tgatcaaaac aatggtgatc accagcataa caattgacat tgaagaggcg
     2941 taagagaccc gacctgctga accaattccc taccaataag catacatatc caaagtttcg
     3001 gtagcaatac caggaccacc tgcagtcagc acaaatatga aatcaaatga acggagggat
     3061 tccatcattt taataaaaaa gattgagaga aagggtggaa taatcactgg gaagataatg
     3121 aaacgataga gctgccatct tttggctcca tcgagtgagc cagcttcgac aatctccttg
     3181 ggaatatcct gtaaaagaat aatggttaaa gccgcaatca aaaaggacca ctgccagatg
     3241 tcgatagccg ctactgagta aagagcatag gaaggatttt ctaagaagcc aatagggcca
     3301 attctcaaaa agcttaaaaa gtaatttata attccaatcg atgatgaata catcaaccgc
     3361 cacatataag ctgctcccac ccggggaagg agtgcgggta taataaaaat aaataaacaa
     3421 agggttctaa aactcccttt tgcattatca ttgatcaagg gggctaacaa tccagcacca
     3481 aaaaaaaccg aaaatccaac agtaataacc gaccaaagca aggaaatacg gagagaattg
     3541 agaaaacgcg catcagtgaa gagttgaata taatttttca atccgacaaa tcgaatcggg
     3601 cgggaacttc ttaggtccca attttgcagt gaataataaa aagcgttgag tagtggatag
     3661 aaggctaaaa aaaataaaat aataaaagtt ggaatcataa atactaaaaa aaattgtttt
     3721 ctggtcacta ttgagccccc ttcttgttac actgtttaaa tggttgggaa agaagtccaa
     3781 ttattttgat ataatgaatt tagaaaaggg gctccaaaga gcccctttta tttcctaatc
     3841 gtttattatt tccccaagac ttcttttgca taagcaactg catcatccag agcagcctga
     3901 atatcatagg attgaccagc ataagctttg gtgaagagtt cttcaagctt tacacccaga
     3961 taatcaccaa tttctggcca ttctggtatt tgcaaaatac acatttgttg aattttcatc
     4021 atctctactt gggcttcaac aaattctgct ggataagcag cggcaaattc ggggctggca
     4081 aaagttgatt ttcgggcgtt agtgagacca gagccagcta caacctttct gatttcattt
     4141 tctttgctgg taaaccaacc gatgaattta ccagcagcaa ctttcagctc ttcagttgga
     4201 caacctgcaa ctgaaatccc caaaccatga accgcagtat tggagatggc acccatggga
     4261 ccggcggctg gttttgagta gcctactttg tcgtatacga ctgatccttt gggatccata
     4321 gtgtaatcca ttttcaggtt ggcgtattca acaatagcaa ccttaccggc attaaagtta
     4381 tccatggcgt ctgaccatga aagggtagga ctggggctgt gtttaatgag ctcaatataa
     4441 tactcaaccg ctttaacggc ttcgggtgaa ttaaaaactg gaacaccatt ttcatccaga
     4501 tactgaccac caaacatctt gagatagggg ctgaatctcc aaacattcat tcctgatccg
     4561 cgaagtcctc ggacggcggt tccgtataac tcaggaggat tgtgaaaatg tttgacaact
     4621 tctaaatatt cgtcgagggt ttgtggaaca gcaattccat ctctttcaaa gaggtctttc
     4681 cgataaaaca tcacatttcc ttcaaatgtc attggagcgc cgtaggtaat gtcattaaaa
     4741 gttagaacag ccactaatgg ctcagaaaaa tcagcgaaat catattcctc agggtaataa
     4801 tctttaatgg ggattaacca gttgttgact gcaaactcag cgacgttagc ttcgtcgata
     4861 taatacactt gatattgacc ggctttggtt gacgcatcaa ggcgagattt tgatcttctt
     4921 tctaattcag caaaatagtg aaaaacaaca tcgatatttt ctttttcttc aaattctgga
     4981 aggtattgtt caagaagctc ccattcacgt cggggttggg tgagaacatt tatgataact
     5041 ttattctgtg cggagactat accgggtatt aacaataaac ccatggttat tgtgattaat
     5101 aagagtataa ataatgattt tttcattctc tctgcctcct ttaataatta aagaaaatta
     5161 taaaaatgct gctttagtca atttttccac tatcacctcc taatttaaaa aattcatcat
     5221 tataaccaat atacatgaca aacttaaaaa aaacatcgaa atctcaaaat atatcaattt
     5281 ttcaatttga ggtattaaaa gaatttttat ttattgtaat aatattaaat atcttaatta
     5341 ataaaaggta cgtaaatgga ggttggttaa aaatagttat aaaaatcctt cgtcactcag
     5401 aatgtcggct acccaggagg cagccatagc tactaccgtg gtacatttat caagatgagc
     5461 tccagcttct tcgaactctt ttcgaacttc ttttgatctt aaacaaaagg attttccgaa
     5521 gatttgtttg tggatgtcag cacaaatcac acttccatag gtttcaataa atttatcatg
     5581 aagtctttgc gccatttggt aggcttggga ttgtgcctct ttatctccat taacttctaa
     5641 catctcaaag cgtcttccaa taaaacttcc cataatcatt atacctcccg aatagccacc
     5701 acaggctcca tctccacaaa gcgccattcc acccgaaagc ccacttgcag attgaaaaag
     5761 ggagttattg tttttcccgg taaatttaaa gatggttgag atgaggcatt gagcgcaccc
     5821 acgataattt ttttcgtatt caaagccaat
//
LOCUS       DAOSOL010000059         5841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_13828_length_5841_cov_3.904252, whole genome shotgun sequence.
ACCESSION   DAOSOL010000059 DAOSOL010000000
VERSION     DAOSOL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13828_length_5841_cov_3.904252"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3488)
                     /locus_tag="PK171_02400"
     CDS             complement(<1..3488)
                     /locus_tag="PK171_02400"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOT05086.1"
                     /translation="MKRFIIALTILLVFLVGIFFIGFQRLASITIDRLLNQFNTGPIQ
                     LSYQQVSFSFPLNIKIVEVSLQNNDFFFHAPQVNLSFDWGLFFGKKESIVAAIPEAVV
                     SSHSKDFIQNIKDFQFHIVPDFEIDIKSLSVPNDTHTLDAFPLFLQKKDKIISFRINH
                     PELSMSGNFISEKEIQAEYKILSKNYPLQGQLIFSLNDDTLKGTVESEMDFSFSALLV
                     PKEDGIELQDFSIQNISDEKNSIKFFGNSRLWLKDQPKIQINGQVRSGMVENAYDFDL
                     ELQRKNISEIVGSLHLKAGEPNIDLISSVVFLPESSSFSLQLLPGSIIEENPILGEIK
                     GKYSDQQINVEFVSHPMEIQFAELTGKGIINGNATFSSGSFKAEGQLNSEWLQYQNYR
                     IEKIQMQFQYDLERILFQGVGYLFDGKLTLDGSYFNKQFNARGTIENSKLDYFIGGDN
                     LPFTGNVSGVFEVQKVDGDPLLISLSLSDGTLFWKDIFIGDQLTAQLNYWGDKLTITD
                     LMLRNQDGTIEGEIEKEFKNIKGKLRFKNFFISYPIAGKALQMIGEGDGEFQYSEGDW
                     EISANLLAPTWSYDEWSGTDFQFQGEISPETILIEKCETQLLGGNLSLMGEITPYRAI
                     NITGTISNLEFPENDYDFQGKIQEILLSAQGAWDKVLFGINAQGEGLTYQGQPLGTTF
                     QITIEGESALPKQNEEIGFERYLNPEILKKGNIQIQGVHFEQEIEDGYKVAGQGDIEA
                     QLDVSNKIWILKSQNLNLQVEEFLNFNGSVQGSFKNDQIEIQQLHLKEITRNIDILGS
                     GSYNVKDKSIQGQFIGQYDTNLPFPDYGLDVYLAGNTELSLTGTMDKPEYQGWIQVNQ
                     GKVVQQEKDLIQLEDVFATINGKELTISEGHGQWLGFDFNTEGTIGETGIKLAFDISG
                     ENGLLGQFDYFQGSWQGKITLQGEFSDLQAEGMIQVRNGFIDTTHLDKNQSTTTIFQN
                     LNESFLNIPIQLKLILKTDDNFRVKTRFLDLKLKGGLTLENGQGTMEIKGRLDVTEGT
                     YDLVMRTIPVQGYVVFGDYFGFIPQIHLEGRIEVRRYDIILKADGPLDDYEMKLQSEP
                     PLIQEEILSLLFVGDKDAYQSLDTLDLEPHLLSALRFFLGMKGTGLKNVLFFDSVELV
                     TPNQENEGFFGIELKK"
     gene            complement(3493..5106)
                     /locus_tag="PK171_02405"
     CDS             complement(3493..5106)
                     /locus_tag="PK171_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HOT05087.1"
                     /translation="MKAKQLGWIFLIFFFTILIVFFPYSSYAEKVPFFPIQSVRRNME
                     GIGKSVFYGTRIENFAVEVLDVVIGKDINQSYIVVKVTDEKIKKMGGISAGMSGSPIF
                     FSGKLAGALAYSWETKDNLIGVVTPIEAMLKLWENMPESSTVLQVAPSSVIFTIGLSE
                     RAGKRLQEKASFFSRKIISLPATIYSQRSNLPSVEIQPGSAIGVQLIHGDVDVVSLGT
                     LTWRDENKILAFGHPFLHQGRVNYFLSSMYVNFSLEGKDFPFKVGTPIQPIGIVDEDR
                     SAGIAGRLGVLPKVIKADIEIGSEKSVISQNYFEIIQDENVVVEFFPEIILNSIDQAI
                     DSQRPGSVKVTLSIEGKEFNFQNEFFWVSKIDISSYTSNNLGKILEDVFKNPFQSIKA
                     DKISIKIIFIPDIREATFRNLFLPVDVKRGNDLKGRIDLNLYRQGVKSLDFGLFVPRD
                     FVPGEAEVVVRGMSWGDENTQSPSPQADLDNYIDEKVKQLKSNGLIIEMRSKSETFSS
                     DNQKNFFSEVVSLPFVLEGSFTGSVVVLE"
     gene            complement(5110..>5841)
                     /locus_tag="PK171_02410"
     CDS             complement(5110..>5841)
                     /locus_tag="PK171_02410"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT05088.1"
                     /translation="DIVICVPSGGTEVEKRAVLDAAYHAGARKAFLIEEPMAAALGAG
                     LEIAEASGNMVIDVGGGTTDIAVLSLGGVVISQSVRVGGDKLDEAIIRHLRKKFNLMI
                     GERTAEILKIEIGWAVPPREEKAADVKGRDLVSGLPKTITITASEVYKCLSEPLSSIV
                     DAARIILEHTPPELAADIGEKGICLTGGGALLQGMDEMIGKALATPTYVAEEPVSCVA
                     LGAGKVLDNLKSLREGFLYTKRRNS"
BASE COUNT         1856 a         1255 c          880 g         1850 t
ORIGIN      
        1 tcttttttta attctatacc aaaaaatcct tcattctctt gatttggagt aaccaattca
       61 acactatcaa aaaaaagaac attttttaaa ccagtacctt tcattcctaa gaaaaaacgc
      121 aaagcgctta atagatgtgg ttccaagtct aaagtatcta aagattgata agcatcttta
      181 tcaccaacga ataagagaga tagtatttct tcttgaataa gaggcggttc agactgaagc
      241 ttcatttcat aatcatctaa aggaccatca gctttcagaa taatatcata tcgccttact
      301 tcaatacgcc cttccaaatg aatttgggga ataaaaccaa aataatctcc aaacaccaca
      361 tatccttgaa cgggaatagt tctcataact aagtcatagg ttccttcagt cacatccaaa
      421 cgacctttaa tttccatagt accttgacca ttttcaaggg ttaatccacc tttaagtttt
      481 aaatctaaaa accgggtctt tactcgaaaa ttatcatctg tttttaaaat aagtttcaat
      541 tgaattggaa tatttaggaa tgactcattc aaattttgaa aaattgtcgt ggtactctgg
      601 tttttatcta aatgagttgt atcgataaaa ccatttctca cttgaatcat cccttcggct
      661 tggaggtcag aaaattcacc ttgaagagta attttaccct gccaagaacc ttgaaaataa
      721 tcaaattgtc ctaacaagcc attttctccg ctaatatcaa aagctaattt gattcccgtt
      781 tcccctatcg ttccctcggt attaaaatca aaacctaacc attgaccatg accctcggat
      841 atagttaatt ctttaccatt tatcgtagca aaaacatctt ctaactgaat taaatctttt
      901 tcctgttgta caaccttccc ttggtttact tgaatccaac cttgatattc tggtttatcc
      961 atggtacctg ttaggctcaa ctcagtattg ccggccaaat acacatcgag accataatca
     1021 gggaaaggaa gattggtatc atattgacca ataaattggc cttgaatgct tttatccttg
     1081 acgttgtaag atccagaacc aagaatatcg atatttcgag taatttcttt taaatggagt
     1141 tgttgaattt caatttgatc atttttaaaa cttccctgaa cagaaccatt aaaattcaaa
     1201 aattcttcga cctgaagatt aagattttgg ctcttaagga tccaaatttt gttagaaaca
     1261 tcgagctgtg cttcaatatc tccctgccct gctactttat aaccatcttc gatttcctgt
     1321 tcaaaatgaa caccttgaat ctgaatattc ccttttttaa gaatttcagg atttaaatac
     1381 ctttcgaaac caatttcctc attttgttta ggaagagcac tttcaccttc aatcgtaatc
     1441 tgaaaagtag ttcccagtgg ctgtccttga taggttaatc cctctccctg agcattgatc
     1501 ccaaaaagca ccttatccca tgctccctga gcactcagta gtatttcttg aattttaccc
     1561 tgaaaatcat agtcattttc aggaaattca agattgctaa ttgtcccggt tatatttatt
     1621 gctctatagg gcgttatttc gcccatcaga gacaaattac cacccaatag ttgagtctcg
     1681 catttttcta tgagtattgt ttccggcgat atttcacctt gaaattgaaa atcagttcca
     1741 gaccactcgt cataactcca ggttggtgca aggagattgg ctgaaatttc ccaatctcct
     1801 tctgagtatt gaaattctcc atccccttca ccaatcattt ggagagcttt cccagcaata
     1861 ggataagaaa taaaaaagtt tttaaaacga agttttccct taatattctt aaactctttt
     1921 tcgatctccc cttcaattgt cccatcttgg tttcgaagca ttaaatcagt tatggtcaac
     1981 ttatcacccc agtaatttaa ctgagcagtt aattgatctc caataaaaat atctttccaa
     2041 aaaagcgttc catcggaaag ggataaggaa atcaaaagtg gatccccatc cactttttgg
     2101 acttcaaata caccagaaac attccctgtg aatggaagat tgtctccacc gataaaataa
     2161 tctaatttac tattttcaat agtacccctc gcattaaatt gcttattaaa ataggaacca
     2221 tccagggtta attttccatc gaataaataa ccaactcctt gaaatagtat tctctcaaga
     2281 tcatactgaa actgcatttg aattttttcg attcgataat tctggtattg aagccattcg
     2341 ctattcagtt gcccttctgc cttgaaagat cctgaagaaa aagtggcatt cccattgatt
     2401 atgccttttc cagttaactc ggcaaattgg atctccattg gatgggaaac aaattctaca
     2461 tttatttgtt gatcggaata tttcccttta atttctccaa gaatcggatt ttcttcaatg
     2521 attgatccag gaagaagctg aagagaaaaa cttgagctct caggcaaaaa aaccacactt
     2581 gaaataagat caatgttggg ctctcctgct tttaaatgga ggctcccaac aatttcagaa
     2641 atgtttttac gttgaagttc aagatcgaaa tcgtaagcat tttcaaccat tccactcctt
     2701 acctgtccgt ttatttggat tttaggctga tctttaagcc acaatcggct atttccaaaa
     2761 aatttgatac tatttttttc atcagaaata ttctgaatgc taaaatcttg gagttcaatt
     2821 ccatcttctt tcggaactaa taaagcagaa aaagagaaat ccatttcgct ttcaacagtc
     2881 ccttttaaag tatcatcatt taaagaaaaa atcagttgac cctggagagg gtaattctta
     2941 ctgagaattt tatattcagc ttgaatttct ttttctgata taaaattacc actcatagat
     3001 aattcaggat gatttatgcg aaaggatatt attttatctt tcttttgaag aaacaagggg
     3061 aaagcatcta aagtgtgggt gtcgttgggg acactcaggc ttttaatatc aatttcgaaa
     3121 tcaggaacaa tatgaaactg aaaatcctta atattctgta taaaatcttt tgaatgagat
     3181 gagactacgg cttcaggaat agctgcaaca atgctctctt ttttaccaaa aaagagaccc
     3241 caatcaaaag aaaggttaac ttggggagcg tgaaagaaaa aatcattatt ttgtaaagat
     3301 acctcaacaa ttttaatgtt cagaggaaat gaaaaagaca cctgttggta actcagttga
     3361 atcggtccgg tattgaattg atttaacaag cgatcaatgg taatgcttgc taaacgttga
     3421 aaaccaataa agaaaattcc aactaaaaaa actaacaata tcgttagagc aattataaac
     3481 cttttcaaga ttttactcca aaaccactac acttcccgta aaagaacctt ctaaaacaaa
     3541 ggggagtgaa acgacttcag aaaaaaagtt tttctgatta tcgctggaaa aggtttcact
     3601 tttgcttctc atttcaatta taagaccatt cgatttcaat tgctttactt tttcatcgat
     3661 ataattatca agatcagctt gtggagaggg tgattgagta ttttcatcac cccaactcat
     3721 acctcgaact actacctcag cttcacccgg aacaaaatct ctcggaacaa acaaaccaaa
     3781 atcgagactt ttaacgcctt gacgatagag gtttaaatca attcttcctt ttaaatcatt
     3841 tcctcttttt acatcaaccg gtaaaaagag attcctaaag gtagcttctc gtatatccgg
     3901 aataaatata attttgatac ttattttatc ggcttttatc gattgaaaag gatttttaaa
     3961 aacatcttca agaatttttc ccagattatt cgaggtataa ctggaaatat ctatcttact
     4021 cacccaaaaa aattcattct gaaagttaaa ttcctttccc tcaatcgata atgtcacttt
     4081 aacgctcccc ggtctttggc tgtcaatcgc ctggtcgata gaatttaaaa taatttctgg
     4141 aaaaaattct accacaacat tttcgtcttg aataatctca aagtaatttt gagatataac
     4201 gcttttttca ctaccgattt caatatcagc ttttattact ttgggcaaca ctcccaatcg
     4261 accagctatt cccgcacttc ggtcctcatc aacaatccct atcggttgaa tgggcgtgcc
     4321 cactttaaag ggaaaatctt tgccttctaa gctaaaatta acatacattg aagataaaaa
     4381 ataattcacc cttccttgat gtaagaaggg atgaccaaag gctaaaattt tattttcatc
     4441 tcgccaggtt aacgtcccca aactgactac atccacatca ccatgtatca attgaacacc
     4501 aatagcgctc cctggttgaa tttctactga tggtaagttc gaacgttggc tatatatagt
     4561 agcaggtaaa gaaataattt tccgagaaaa aaaactcgcc ttttcttgaa gtctttttcc
     4621 ggccctttca ctcaatccaa ttgtaaaaat gaccgagcta ggggcaacct gtaaaaccgt
     4681 tgaagactcc ggcatatttt cccagagctt aagcatggct tcaattggag tcactactcc
     4741 gattaagtta tctttggttt cccaactata ggctaaggct ccagctaatt tcccactaaa
     4801 aaaaatagga cttccgctca tgccagctga aatcccaccc attttcttta ttttttcatc
     4861 agtaacttta accacaatat agctttgatt gatatctttc ccaataacaa catccaatac
     4921 ttcaacagca aaattctcaa tcctggtccc ataaaaaacg cttttaccta tcccttccat
     4981 attacgccga accgactgga tcgggaaaaa ggggaccttc tcagcataac tagaataggg
     5041 aaaaaatact attaaaatgg taaagaaaaa tatgaggaat atccatccta attgtttcgc
     5101 tttcaagaac taactgttcc tcctttttgt atacaagaaa ccttcccgga gggactttaa
     5161 attatccaac actttccctg ctcctaaggc cacacaggaa accggctctt cggccacata
     5221 agttggagtt gccagtgcct ttccaatcat ctcgtccatt ccttgaagaa gtgctcctcc
     5281 gccagttaaa catattcctt tttctccaat atcagcagct aattctggag gagtgtgctc
     5341 taaaataata cgggcggcat ctacaattga tgagagtggt tctgaaagac atttataaac
     5401 ctcggaagca gtaatggtga tcgttttagg aaggcccgac accaaatctc gtcctttcac
     5461 gtcagcagct ttttcttccc gcggtggtac tgcccatcca atttcaatct taagaatctc
     5521 agcagtacgt tcgccaatca taaggttaaa ttttttccga aggtgtcgaa taattgcttc
     5581 gtctaatttg tctcctccca ccctaaccga ttgactgata actacaccac ccaaagaaag
     5641 aactgcaata tcagtcgttc ctccaccgac atcaataacc atatttcccg aggcttctgc
     5701 gatttctaaa ccagctccta aagcagctgc cattggttct tcaatgagaa acgcctttct
     5761 tgcaccagcg tgataagcag catctaaaac cgctcttttc tcaacttctg ttccacctga
     5821 aggcacacat atgacaatat c
//
LOCUS       DAOSOL010000060         5793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_13987_length_5793_cov_2.638376, whole genome shotgun sequence.
ACCESSION   DAOSOL010000060 DAOSOL010000000
VERSION     DAOSOL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13987_length_5793_cov_2.638376"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1243)
                     /locus_tag="PK171_02415"
     CDS             complement(74..1243)
                     /locus_tag="PK171_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOT05089.1"
                     /translation="MLLPEYEMKSRLKKVQDLLVTKDLPGTLVYYDELNIANGWYLSG
                     WCPQFESGCLFIPRQGEPMILGGPESEPFAKTDSAIKKTKNIPVFMVPEEEYPNATIS
                     SFAEVFDEIGIKGKSQKLGVVGLDKMPFGVYQLLKKDLLGIDLVDITTEYEQFRKIKS
                     PWEQEQIRKAFAIADQSFQMMKPLVREGVSEIEIAGAGEGKARSLGANWFAFKAIVAS
                     GARTSGVVPTASDKKLQVGETVMMGLSPRYNGYAGVFGYTMVVDGKFNEAQRRCINDM
                     VEAYRITKSNLKPGMIGKDINAVTHEFVTKQGYGKNIVCPFAHTIGLMEAEAPFFGPN
                     SNDILQAGMTVCVDVSVFSVPEANGVRFESAFLITDKGPEPLSPYMDQLILNTKV"
     gene            complement(1274..2305)
                     /locus_tag="PK171_02420"
     CDS             complement(1274..2305)
                     /locus_tag="PK171_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT05090.1"
                     /translation="MPPTIRDVAREAGVSVATVSRVINGSEAVSDEIKDRVLKAINLL
                     NYQPNLSARFLSQKKHPFSDDTKYVGVLFGEYVHSDHYFFSSVISGIEKTMFEKRLNV
                     VISSVSQRDNYTPLDLPLFIAEKSLKYLIVIGETDPKFLFYLKENGFIVVMVDDIGPI
                     GFDCVLCDYKRGALEAVEYLINLGHKNIGLIAGAEHHYFSRALENSYLKVLQSHGIPI
                     RREYIVYNEDFNVDGGARSMEKLFLLSNPPTAILTNDEMGIGVLQKANEMNISIPDRL
                     SVMGFDDIEMARFFHPPLTTMKIPGSEMGRLAAKLVIDKMQENEPLPAQRIELSPILV
                     ERKSCISLK"
     gene            complement(2341..2751)
                     /locus_tag="PK171_02425"
     CDS             complement(2341..2751)
                     /locus_tag="PK171_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05091.1"
                     /translation="MDTRRNLSILEIPNFADLTIESHDQYGRDRDQWPLFRTEGAESL
                     MKHVNSMIAYCADRNLPAVFCLYIHPWEFIEMPSRLSYGEATVEPLQFIIKNCGPRAL
                     TELSKVIDAFKDKGAHFYSAAGLAHQWRESVKDS"
     gene            complement(2691..3239)
                     /locus_tag="PK171_02430"
     CDS             complement(2691..3239)
                     /locus_tag="PK171_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT05092.1"
                     /translation="MDISVVIGVDTETDIGSFTPFYNGVEKGTPLLMDLFEKKGIPAT
                     FFMVAEAAQKFPELTREIKQRGFEVGCHTIHHETIGDPLFEIPLVKPVLPEEVPHRLE
                     VATAMIAEVIGEAPQSFRAPRLWGSTIMVNTLEKLGYLADATYPLYYYQERVTPYYPS
                     AKDWTQEGTFPFLKYQILLILL"
     gene            3561..5366
                     /locus_tag="PK171_02435"
     CDS             3561..5366
                     /locus_tag="PK171_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOT05093.1"
                     /translation="MLKKGPLFYILFILFTILAPATLTHALPEFRGVWIDVRSIPNTR
                     DGIEAMVKELASAHFNAILVESFYLGKTIYPSDFLAQQGLTSQMEVYEQTNLDPLAAF
                     IESAHSYNMQVHAWFDMFYVGLNQPGELLEKFPAWQSIQRDGTVGYTQGKNHFYWACP
                     LHQGVKEFYIGLLIEVLQNYDLDGIHLDYLRFPDTTIADTCYAQEHREHFLSQYGVDP
                     IAIDPFTQPDVYRLWNSYRAQSVTGLLAEINTRVHQIKPDIILSVAVQPRGMPIELNP
                     GFLQNWPFWTENHYLDVLIPMTYSSRVNEMKGLTYWVNTFLLGNLPAFAGIQAFNLPD
                     IKNLSQMVEFSRLAQFQGSVIFAYPYLNSEILDSLKQGPFQEKAEAPSRKYLNSLVPV
                     EKVQVVQYSHPNPRTIKAQLIHEVIQVDGLLHEKSWEKADFQNEFSLITGEGLASSQT
                     TVACLFSPTYLYIMFQVDDSPAQNRKITIYQKDGPVFYDDSVEVFIDPWFSSSFYYHL
                     SLNSIGTQYDSFSRSGPSWNANWEVAIQEKTNGWTVEIAIPFSKLEIQTPQPGDQWKI
                     NFNRTDIILGEFSGWSPTPGTFHAPSFFGVLEFVK"
BASE COUNT         1636 a         1328 c         1064 g         1765 t
ORIGIN      
        1 ttttaaaaat attgatttta tcaggttcaa tatcaccccc taatcaagaa aaagcctttc
       61 cacggcagaa gtattaaact ttggtattca atatcaattg atccatatag ggcgaaagag
      121 gctcaggacc tttatcggta atgagaaagg cactttcaaa tcgaacccca tttgcttccg
      181 ggaccgaaaa gacactcaca tccacacaga ctgtcatacc ggcctgaaga atatcattgc
      241 tattaggccc aaaaaaggga gcttcagctt ccattagccc tatagtatga gcaaagggac
      301 agacgatatt ttttccatac ccttgtttgg ttacaaattc atgggttaca gcattaatat
      361 cttttccaat catccctggc tttaaattgg atttagtaat ccggtaggct tcaaccatat
      421 cgttgatgca gcgtctttgt gcttcattga actttccatc aaccaccatg gtatagccaa
      481 atactcctgc ataaccattg tatcgagggc tgagtcccat catcaccgtt tcaccaactt
      541 gcaatttctt gtccgaagct gtaggaacta ctccgctggt tcgcgctccg ctggcaacaa
      601 tagctttaaa ggcaaaccaa ttcgccccta aggatcgggc tttcccctct ccagctccag
      661 caatttcgat ctctgaaaca ccttcacgaa ccaggggctt catcatctga aaagattggt
      721 cggcaatagc aaaggctttt ctgatctgtt cttgttccca gggtgactta atcttccgaa
      781 attgttcata ttcagtagtg atatcaacca ggtcaattcc caataaatct tttttaagta
      841 actggtaaac tccgaagggc atcttatcca gcccaaccac acccagtttt tggcttttcc
      901 ctttaatgcc aatttcatca aagacttcgg caaaagaaga tatggtagca ttcggatatt
      961 cctcctctgg aaccataaaa accggtatat ttttggtctt cttaattgct gaatcggttt
     1021 tggcaaaagg ttccgattct ggtcctccca aaatcattgg ttcaccttga cggggaataa
     1081 acaggcagcc actctcgaat tgtggacacc aaccggataa ataccaccca ttggctatat
     1141 tgagttcatc gtaatatacc aaagtccctg gaagatcttt tgtgacaaga aggtcttgta
     1201 ctttttttaa gcgagatttc atttcatatt ctggtaataa catattcctt tctctccttt
     1261 tactttatgt tttttatttc aatgatatac atgattttct ctcaacaaga ataggagaca
     1321 actcaatccg ttgagcggga aggggttcat tttcctgcat tttatcaatg accaattttg
     1381 ccgctaaacg ccccatttct gatcccggaa ttttcatggt tgtaagcggt ggatggaaaa
     1441 atctcgccat ctcaatatca tcaaatccca tcaccgataa tcggtctgga atagaaatat
     1501 tcatttcatt ggctttttga agaactccta ttcccatttc atcattggtt aaaatggcag
     1561 taggtggatt ggatagcaag aaaagtttct ccatactccg tgctccccca tcaacgttaa
     1621 aatcctcatt gtaaacgata tattcacggc ggattgggat accatgagat tgcaggactt
     1681 ttaagtagct gttttctaac gctcgagaaa aataatgatg ttccgctccg gctattaagc
     1741 caatattttt atgacctaaa tttatgagat attcgactgc ctccagtgcc cctcttttat
     1801 aatcacagag aacacaatcg aagccaattg gaccgatatc atccaccatt accacaataa
     1861 aaccgttttc cttaaggtaa aaaagaaatt ttggatcggt ttctccaatg acgattaagt
     1921 atttcagact tttttctgca ataaaaaggg gaagatcgag aggagtgtaa ttatcccgtt
     1981 gggatactga tgatatcact acgttaagcc gcttctcgaa catggttttt tcaatcccag
     2041 agataacact tgaaaaaaaa taatgatcag aatgaacgta ttcaccaaat agcacgccaa
     2101 catatttagt atcatcggaa aaaggatgtt ttttttggct taaaaaccga gcggacagat
     2161 tgggttgata gtttaaaagg ttgattgctt tcaatacccg gtctttgatt tcatcactga
     2221 cggcttctga gccattgatt acccttgaaa ccgttgcaac acttacacca gcttctcggg
     2281 ctacatctcg aatggttggt ggcatagtct ctcctttagg ttctttttgt gtaaccgttt
     2341 tcagctatct ttcacacttt ctctccattg atgagccagt cctgctgccg aataaaaatg
     2401 tgcaccttta tctttaaaag catcaatcac tttagatagt tcagttaagg ctcggggccc
     2461 acagtttttg ataataaatt gaagaggctc aacggtggcc tctccataag aaaggcgaga
     2521 aggcatttca atgaattccc agggatggat atataaacaa aataccgccg gcaggtttct
     2581 atcggcacag taagctatca tactattgac atgcttcatc aatgattcag cgccttcggt
     2641 tcggaaaaga ggccattgat ctcgatcccg accatactga tcatgacttt ctatagtaag
     2701 atcagcaaaa tttggtattt caagaatgga aaggttcctt cttgtgtcca atcttttgct
     2761 gatggatagt aaggagtaac tctttcttga tagtaataaa gcggataagt agcatcggcg
     2821 agatacccca atttctctaa ggtattcacc ataatagtac ttccccaaag tctgggagct
     2881 cgaaaagact ggggagcttc tccaataact tcagcaatca ttgccgtagc tacttcaagg
     2941 cgatgaggta cttcttcggg taaaactggc ttgaccaacg gaatctcaaa aagtggatcg
     3001 ccaatcgtct cgtgatgaat ggtgtgacag cccacttcaa aacctctttg tttaatctca
     3061 cgagtgagtt ccgggaattt ttgggctgcc tcagcaacca taaaaaaagt agcagggatt
     3121 ccttttttct caaacaaatc catcaaaagc ggtgttcctt tttcaactcc attataaaaa
     3181 ggcgtaaaac tccctatatc agtttcggta tctactccaa taacaactga aatatccatc
     3241 ttttaccctc ttttctttta aatataaaca attgtaaacg aatacgtaac cgttatcatt
     3301 ttaactaaaa aatacaaaat atgtcaatat ctaacctgaa aaccccatca aacaaattcc
     3361 cccattgagg ggggttagtg gggtgtgcct ttaatcggtc atcctgagcc ccttttcccc
     3421 tctccttttt ctcctcctcg ctccctctcc ccctcagttt tttcttgtcc ccttactcta
     3481 ttttctcaag ctattgcatt tcagatctgg ctttggaata atgaataaaa ttccaactca
     3541 acctgaagga gaacactccc atgctaaaaa aaggccctct attttatatt ttattcatcc
     3601 tttttactat tctggctcct gcaaccttga ctcatgccct ccctgagttc cgtggagtgt
     3661 ggatcgatgt gagaagcatt cccaataccc gagatggcat tgaagccatg gtcaaagaat
     3721 tggcatcagc tcatttcaat gctattctgg ttgaatcttt ttatctgggt aaaaccatat
     3781 atccttcaga ttttttagct caacaagggc ttaccagcca aatggaggta tatgaacaaa
     3841 ccaatcttga ccctcttgca gcctttatcg aatcagctca tagttacaat atgcaggtgc
     3901 atgcctggtt tgacatgttt tatgtcggtc ttaaccaacc tggagagctg ctggaaaagt
     3961 ttcctgcttg gcaatccatt cagcgtgatg gaactgtcgg ttatacccag gggaaaaatc
     4021 atttttattg ggcctgtcct ctccatcagg gagtaaaaga attttatatc ggacttctta
     4081 ttgaggttct tcaaaattat gacctggatg gaattcacct tgactatctg cgattcccgg
     4141 atacaaccat tgccgacacc tgttacgctc aagagcatcg ggaacacttt ttatcacaat
     4201 acggcgttga tccaattgca atcgatccct tcacccaacc ggatgtctat cgtctctgga
     4261 attcttatcg agcccaatcg gtcactggcc tcctggcaga aataaataca agagttcacc
     4321 aaattaaacc ggacatcatc ctttcagtag ctgttcaacc ccgagggatg ccaatcgagc
     4381 tcaatcccgg attcctgcaa aactggcctt tttggacgga aaatcattac ttggatgtat
     4441 tgatccctat gacttacagc tcaagagtaa atgagatgaa aggattaact tattgggtca
     4501 atactttttt attagggaat ctgccggctt ttgccgggat ccaagccttt aatcttcccg
     4561 atattaaaaa cctctcccaa atggtcgaat tctcacgctt ggctcaattt caaggttcag
     4621 tcatctttgc atacccctat ctaaacagcg aaatactcga ttccttaaaa caaggaccct
     4681 tccaagaaaa agccgaagct ccatctcgaa aatacctcaa ttctctggtt ccagttgaga
     4741 aagtccaagt cgttcagtat tctcacccca atccccgaac catcaaagct caactcattc
     4801 atgaagtaat ccaggtcgat ggattacttc atgaaaaatc ctgggaaaaa gccgactttc
     4861 aaaatgaatt ttcccttatc actggagaag ggttggccag cagccaaacc accgtagctt
     4921 gtcttttttc acccacttac ctctatatca tgtttcaggt agatgattct cctgcccaaa
     4981 atcgcaaaat caccatttac caaaaggacg gaccagtttt ttacgatgat tcggttgagg
     5041 tttttattga tccctggttt agcagcagct tttactatca tttatccttg aattctattg
     5101 gaactcaata tgatagtttt tctcgatctg gaccttcctg gaatgccaac tgggaagtcg
     5161 ctatccagga aaaaaccaac ggctggacgg tagaaattgc tattcctttt tccaaattgg
     5221 aaatccaaac tccgcaaccg ggagatcagt ggaaaatcaa ttttaatcga accgatatca
     5281 ttctggggga atttagtggg tggtcaccaa cacccgggac ctttcacgca ccatcattct
     5341 ttggcgttct cgagtttgta aaatgagagt ctaaacgaca aaccttttgt aagggcgcaa
     5401 tttattgcgc ccgttcctta tttaatttag cgatatgcca tggcatatcg aatctttatt
     5461 tacatcctca tctggtactg caaatcagta tgactgtcta tcctgaaaat atcatcaaat
     5521 gaattccctc attgaggagg gttagggggg tgtgaattta accttgccat cctgagccct
     5581 cgcttggtga gggcgtgagg atctcattaa cccaatccgt cattgcgagg aatgtattgt
     5641 atgacgtggc aatctcttga gaataatctt tttttgtttc ttctcccttt tttgaaagga
     5701 tatttgagag ggattcgatt cgtctccaaa aagtcaaacc cccctttttt tcctttgcta
     5761 aaataggaga ttggttccta aataattggc ttc
//
LOCUS       DAOSOL010000061         5704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14296_length_5704_cov_3.186051, whole genome shotgun sequence.
ACCESSION   DAOSOL010000061 DAOSOL010000000
VERSION     DAOSOL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14296_length_5704_cov_3.186051"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..714)
                     /locus_tag="PK171_02440"
     CDS             complement(1..714)
                     /locus_tag="PK171_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05094.1"
                     /translation="MAGSYLPPESVEKLRDELLKLYGLEGSMFQQYFTYWRELLKGNL
                     GPSFASFPTPVITLIYRSLPWTAGLLVLTAMIAWVIGTILGGIATYRSKSLWVKIFEM
                     LSNAVRPIPYYIFALALLIVFAYILRWFPAGGAFQIGIEPSYSWVFLKSVAKHGFLPA
                     LSLIIIGMGAWFLNMRNLTSNIIHEDFVVFAKQMKLKDQMILRNYVMKTAMPAQLTGL
                     ALQLGTVFSGTIITEIVFN"
     gene            complement(970..2892)
                     /locus_tag="PK171_02445"
     CDS             complement(970..2892)
                     /locus_tag="PK171_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT05095.1"
                     /translation="MLRWRFVCLITIVFLVLGTVAFAQEILPGIPRNEVIVIEDPSGR
                     SLDPGRFNLWGGGLLSYSTGLQQLCLGALWYIDPDEGIEGDPWINLLAAEPPIYNEDY
                     TEMTVKVREGIHWGDGVPFTIDDVIYTVELLKAHPEMIWGAYMQVNVAESQRIDDFTV
                     KFKLTKPNGRFHTVFTVRWSACYILPKHIFEKAEDPLAFAFNPPIGTGPYVLKSFDPN
                     GYWFLWELREDWDKSDLGVAYGIKPGPKYVLYKGIASPEKKVMDQMAHELDIVHDLPP
                     EGAITLYQNNPQCVTWFDGWPWAHPDPTLPSLIMNHEHFPYNLKEVRWALTLSLDIVQ
                     VALQAYNGCATVSPIHVPPTGLYPGWYFDKIEPWLKDFTLKVGGEDYKPYDPEISTKI
                     AEAVRYAFGHKIPDDPQEIRKLVGYGWWKYDLEAAGKLLEGAGFKKDGNGKWLLPDGT
                     PWKISILCEAATRSVMTRAAAAVAEQWRRFGIDAQIDAVEPTMLWNRLNYGEDPNTIY
                     WAWCIETWGGHPDLFWFLESWHSDYYRPTGELTVAKNPMRYKSEQIDQVVDDLRRTDF
                     FKETDKIIDLGLQYIKTCVEDMPIIPIFSYNVFTVCDETYWTGYPTSSNPYTNPVPNW
                     GNTRFMFIKIQPISKE"
     gene            complement(3010..3981)
                     /locus_tag="PK171_02450"
     CDS             complement(3010..3981)
                     /locus_tag="PK171_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05096.1"
                     /translation="MSDSFLTLKNLTKEFQVGTFFSKVKILAVQDITLELERKPAVFT
                     LVGESGSGKTTLANFLLRVLKPTEGSITFLGKNIESYRKFDFMKLVQPVFQNPYETFN
                     PLKKVETYFHSVIKKLRITNENGHSFEKVIEKALSSVALTWEEVKDKFPNEFSGGQLQ
                     RLSIARALMVNPMLLVADEPVSMLDASLRVFVLNLFRKLKEEKQLYIIYITHDLATAY
                     YISDYIGVMLRGCLVEVGPAEKVLLNPLHPYTILLKESVPNPDEVEIKPETKTKEERI
                     LEIEEFLKQGCKFAFRCSNAKDICKKEIPPDVIIDSVAVKCWLYKNV"
     gene            complement(4598..5602)
                     /locus_tag="PK171_02455"
     CDS             complement(4598..5602)
                     /locus_tag="PK171_02455"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT05097.1"
                     /translation="MVVKKTNIEKPKIPLIDGHNHLEILGSYWMEHSVEELLTMMDEM
                     NVGVIVDLDGMENKEMLITHIERFKAKAPDRFYHMGGIDWSKWEEKGSKFGDWAAKQV
                     EEQVKLGASGIKIWKNLGLHIKDPQGNLVKVDDPRLDPIFETAASLKIPVYMHISDPV
                     AFFDPIDQFNERIDDLGEHPEWSFFGPQFPSFLEVIEQFANRIRRHKKTQFIGCHVAS
                     YAENLTWVSSLLDECPNLCVDISERIGELGRQPYTTRKFFIQYADRIIFGIDRLPDKV
                     WYQIYARFLETDDEYFNYDPTYPPRQGRWFIYGIYLPDEVLGKVYYKNLSRILRINKQ
                     "
BASE COUNT         1765 a         1184 c          986 g         1769 t
ORIGIN      
        1 ttaattaaaa acaatttcag taataatggt accgctaaag actgttccga gttgcagggc
       61 aagtccggtt aattgagcag gcatagcagt tttcatgaca tagtttctta gaatcatttg
      121 gtcttttaac ttcatttgtt tggcaaatac gacaaaatct tcatgtataa tgtttgaagt
      181 gagatttctc atattgagaa accaggcacc catcccaatg attatcaacg ataaagcagg
      241 taagaaacca tgtttggcaa cacttttcaa aaaaacccaa ctgtaagatg gttcaattcc
      301 aatttggaaa gctcctcctg caggaaacca tcgcaaaata taagcaaaca caattaaaag
      361 agcaagggca aaaatgtaat aggggattgg tcgtacagca ttagaaagca tttcaaaaat
      421 tttaacccaa agacttttac ttcggtaagt ggctattcct cctaagatag tccctattac
      481 ccaagctatc atggcagtta aaacgagaag ccctgccgtc caggggagtg atctataaat
      541 caaagtgatg acgggtgttg gaaaagatgc aaaagaaggt cctaaatttc ctttgagcag
      601 ctctcgccag taggtaaaat attgctgaaa catcgatcct tctaaaccat agagttttaa
      661 aagttcatcc ctcaattttt ccacgctctc aggcggtaaa taagatccag ccatttgtac
      721 tctcctaaca gcgacttctg cgggacttgc tccggggatt aatcttggaa ttaaaaaagt
      781 gatggtaatg cctacaaaaa tcaccaacag gcaaacgata aatcttccaa aaaaatattt
      841 tatgaaaaat ttaaaaaaat tactcacaaa attttcctcc tgattgggcc ttcatcctat
      901 aaggaagccc ccgctttccc cattcaaagc ggaggcttca ctcattccaa ttctttccga
      961 agttagcaac tattcttttg aaattggctg tattttaata aacataaatc tggtatttcc
     1021 ccaatttggc actggattgg tatagggatt actcgaggtg ggataaccag tccaataggt
     1081 ttcatcacaa actgtaaata cgttgtaaga gaatattgga attataggca tgtcttctac
     1141 acaggttttt atatactgaa gtccgagatc gataatttta tcagtttctt taaagaaatc
     1201 ggtacgcctt aaatcatcaa caacctggtc aatttgttca cttttatacc gcattggatt
     1261 cttcgccaca gtgagttcgc cagtaggtct ataataatca gaatgccaag attcgaggaa
     1321 ccagaagagg tctgggtgtc ctccccaggt ctcgatacac catgcccaat aaatcgtatt
     1381 gggatcttct ccgtagttta atcggttcca taacatagtt ggttctactg catctatttg
     1441 agcatcgata ccaaacctac gccattgttc agcaaccgct gccgcagctc tagtcattac
     1501 cgatcgggta gccgcttcac acaaaatgga tattttccaa ggggtcccat ctggcaataa
     1561 ccactttcca ttaccatctt ttttgaatcc cgctccctcc aacaatttac cggcggcttc
     1621 gagatcatat ttccaccaac catatccaac cagttttctt atttcctggg gatcatcggg
     1681 tattttatga ccaaatgcat atcttactgc ttcggcaatt ttcgtagaaa tttctggatc
     1741 ataaggttta taatcctctc cacctacttt gagagtaaaa tctttgagcc agggctctat
     1801 cttgtcaaaa taccatcctg ggtataaacc ggtgggagga acgtgaatgg gggatacagt
     1861 agcacaaccg ttataagctt gaagggcaac ctgaactata tccaaggata aggtgagtgc
     1921 ccatctgact tccttaaggt tgtaaggaaa gtgctcatga ttcattatca aggaaggcaa
     1981 ggttggatcg ggatgtgccc aaggccagcc atcaaaccag gtaacacact gtggattatt
     2041 ttgatacaga gttatggcac cttccggagg caggtcgtga acaatatcaa gttcatgcgc
     2101 catctgatcc atgacttttt tctcaggaga cgctattcct ttgtagagaa catatttagg
     2161 tcctggtttt atgccataag ctacaccaag atcggatttg tcccaatctt ctctcaattc
     2221 ccataagaac caatacccat taggatcaaa agattttaaa acgtagggtc cggttccaat
     2281 tggtggatta aaggcaaaag caagaggatc ttcggctttt tcgaagatat gtttgggcag
     2341 aatatagcaa gccgaccatc taactgtaaa aacggtatgg aaccttccat taggcttggt
     2401 cagtttaaat ttaaccgtaa agtcatctat tctttgactt tccgctacat taacctgcat
     2461 gtaagcaccc caaatcattt cggggtgtgc ttttaaaagt tctaccgtat aaataacatc
     2521 atctatagta aatggaacac catcacccca atgtattccc tccctcactt ttactgtcat
     2581 ttctgtgtag tcttcattat aaataggggg ttcagctgct aacaggttaa tccagggatc
     2641 tccttcaata ccttcatcgg ggtcgatata ccacaaggct cccagacata attgttggag
     2701 accggttgaa taacttaata aacctcctcc ccaaaggtta aatctccctg ggtcgagaga
     2761 acgacccgac ggatcttcaa tgacgataac ttcatttctg gggattcctg ggaggatttc
     2821 ctgggcaaaa gcaacggtac ccaacaccaa gaaaactatg gttatcaaac atacaaaacg
     2881 ccaccgtaac acaagatttc cctcctttta aattcttttt ttaaaccaag gtaataaaca
     2941 tttttatcac aaagaaatct caaccactaa ccattcaaaa attctctctt cacacatcaa
     3001 atccctcctt catacatttt tataaagcca acactttacg gcaacactat cgatgattac
     3061 atcaggaggt atttcttttt tacaaatatc tttagcattg gagcacctaa atgcgaactt
     3121 acaaccttgt ttaagaaatt cttcgatttc caaaatccgt tcttccttgg ttttagtctc
     3181 aggctttatt tccacctcat caggatttgg aacagattct ttcagtaaga tagtataagg
     3241 atgaagagga ttgagtaaaa ctttttcggc aggcccaact tctacaagac aacctcgcaa
     3301 cataacgcca atataatcgc taatataata tgccgtagcc aggtcatgag tgatatagat
     3361 gatatagagt tgtttttctt ctttgagttt tctaaataaa ttaagaacaa atactcgaag
     3421 agaagcgtcc aacatggaaa ccggttcatc ggctacaagc agcataggat tgaccatcaa
     3481 tgccctggcg attgataatc tttgcaattg cccaccagaa aattcatttg ggaatttatc
     3541 ctttacctct tcccaggtga gagcaacaga gcttagtgct ttttcaatca ctttttcgaa
     3601 agagtggcca ttttcattag tgatgcggag ctttttaatt actgaatgaa aataggtttc
     3661 cacttttttc aagggattga acgtttcata aggattctga aaaaccggtt gaactagttt
     3721 cataaaatca aattttctat agctctctat gttttttcct aaaaaagtaa ttgaaccttc
     3781 agtaggcttg agaactctga ggaggaaatt agcaagggtg gtttttccac ttccgctttc
     3841 accaactaaa gtgaaaactg ctggttttct ttcaagttcc aaggtaatgt cctgaacagc
     3901 aagtattttt accttagaaa aaaaagtgcc aacttgaaac tctttggtaa gatttttgag
     3961 agtaagaaat gaatcactca cttttcctaa ccctgaatat agactatcga ttcaaaagat
     4021 gttatacctc ttgaataagg aaaacagggt agtagattaa atatctgggg tttttctctt
     4081 attttttaac aatccttgtt gaagaaacgg taagtgtgaa tattttgagg atttttttaa
     4141 ataactttgt tgcaacacct aatgagtcca agggttctat ttatttaacc acactcacta
     4201 agcgatatta cttattctaa taaataaaaa actatcgaga tgtttttttg ctcttattct
     4261 atttatataa atatgactta gtaaattaat taaaacataa tcactctata aaatcaagtt
     4321 gacaatttga agtcaaaata ccttataata aggctcttcc aaaagctaga aaaccatgat
     4381 gaaggttgtc ctaatccaat ctttccttgc gaatacaata cttatggctc gaatcagtca
     4441 tgctcaataa ccaagaagag gtcccttgaa ttcatccacc caaagcactc attctttcaa
     4501 aatttgagcg tgatagaata gagcccagtt gagcattctt attttctact tcataacaaa
     4561 tcagaaaagg aaactttttt tcttggttat tgaaagatta ttgcttatta atccttaata
     4621 ttcgacttag atttttataa taaacttttc ctaatacctc atcgggtaaa taaatcccat
     4681 aaataaacca gcgaccttgt cgtggtgggt aagtgggatc ataattaaaa tattcatcat
     4741 cggtttctaa aaaccgcgca tagatttgat accatacttt atcggggagt cgatcaatcc
     4801 caaaaatgat gcggtcagca tattgtatga aaaactttct tgttgtgtaa ggttgtcttc
     4861 ctaattcacc aattctttct gatatatcca cacataaatt aggacactcg tctaatagag
     4921 aagataccca agtaagattt tcagcataac tggctacatg acaaccaata aactgggttt
     4981 tcttatggcg acgaatccga ttggcaaatt gctctataac ttctaaaaaa gatgggaatt
     5041 ggggaccaaa aaaactccat tcgggatgct ccccaaggtc atcgattctt tcattaaatt
     5101 ggtcaatggg atcaaaaaaa gcaactggat cagagatatg catatacact gggattttca
     5161 acgaggcagc tgtttcgaaa attggatcta atcttggatc gtccactttg actaaatttc
     5221 cttgcgggtc tttaatatgg agccccagat ttttccatat tttaataccc gaagctccca
     5281 attttacttg ttcttcaacc tgcttggccg cccagtctcc aaatttgctt cctttttctt
     5341 cccatttgga ccaatcaatt cctcccatat gataaaaacg gtctggagct ttagctttaa
     5401 atctttctat atgagttatt agcatctcct tgttttccat gccatcaagg tcaacaatca
     5461 cacccacatt catttcatcc atcatcgtca ataattcttc tactgaatgt tccatccagt
     5521 agcttcccaa tatctcgaga tggttgtgac cgtctatcag gggaattttc ggtttttcaa
     5581 tattagtttt cttgacaacc aactttggtg taggatgata ttcagcaagt gtaattttct
     5641 tactaatctt gcatctcctc ctttgttaaa cagttattac aattaaagcc ttttcttata
     5701 gatt
//
LOCUS       DAOSOL010000062         5660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14435_length_5660_cov_4.096521, whole genome shotgun sequence.
ACCESSION   DAOSOL010000062 DAOSOL010000000
VERSION     DAOSOL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14435_length_5660_cov_4.096521"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..2604)
                     /locus_tag="PK171_02460"
     CDS             complement(208..2604)
                     /locus_tag="PK171_02460"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05098.1"
                     /translation="MLYGYFDDQNKEYVIERPDTPQPWINYLGEESYFGIISNTAGGY
                     SFYRDARLRRILRYRYNNIPQDKGGRYLYIRFSDGDFFSPTWQPVRKKLDFYRCRHGL
                     GYTIISSLYRDIESEITYFVPIGEELEIWSVKLFNRGKETLELDVFSFVEFCLWNALD
                     DATNFQRNLNIGEVEVEEGVIYHITEYRERRDHYAFFGAVGNIVGFETDRESFLGPYE
                     SLSSPYSVREGKTSNSIACGWAPVGVHQIKVTLPSQHEERIQFLLGYKENLTQHKFTA
                     ERRVNKEGIPSLLQRFTNHNEVDRALGKLKSYWKNILERFQVQTPNTHFNRMINVWNQ
                     YQCMITFNLSRSASYFESGISRGIGFRDSNQDLLGFVHLIPDRARERLLDLSSTQFQS
                     GGAYHQYQPLTRQGNDEIGAGFNDDPLWLILAAASYLKETGDFTILNEVVSFADGGDP
                     APFYQHLLRSLEYTLHNLGPHGLPLIGQADWNDCLNLNLVTENEGKTAESILIGEMFI
                     AAGEELACIAEIIGKSEDSIRFREETRKMIDKINQWGWDGEWYLRAFDDRGLPVGSKQ
                     NQEGRIFLETQPWAIMSGVANLERGKVTMDSVEKYLATPHGIILQQPAFTQFSLHLGE
                     CTSYPPGLKENAGIFCHPNPWAVIAECKLKRAEKAWNYWLTICPSWREKVSEVHRQEP
                     YVYAQMIAGPDHPHFGEAKNSWLTGTASWALISATQWILGIRPDYQGLIVDPCIPADW
                     KGFQVQRWFRGVQFTIVVERKPNEKYLLKVDGKTISGKTIPIFSPGSEHLVEVILGED
                     "
     gene            complement(2670..3923)
                     /locus_tag="PK171_02465"
     CDS             complement(2670..3923)
                     /locus_tag="PK171_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05099.1"
                     /translation="MFKNIFLWSLIMVFCVSIAVQAAESINFWLMPNAPDDIHVPWLN
                     EAVAEFKEQTGISVTYEIVGWGDAWTRITSAIATGEGVDVFQVGTTWNPQFAATGGLT
                     EIDINEFGGAGAFMKANYISTTYQEKTFGVPWFAETRALFYNKDMFQQAGMEPPQTYQ
                     ELYEAGERINEKFGKGRAISIAGTNAWDLLHNWAIVLWAHGGVLVDEQTKTARFNEAP
                     GVEAMKWYVGLVANGYADEACAEYNQPQADSAFINGNVAMCFMGPWNIANIEVENPDL
                     NYDVVEPPAGPAGRASFSGGSNLVILKASSNQEAAKKWIKFLLEKQKLVDYTQNLSQM
                     LPALLEAYDDPYYETGVWKVFQTTLSYATAYPPLARWGDVENAVQEEFRNILTAYIAK
                     TYSDEKVQEYLDTAADRVNRALARD"
     gene            complement(4064..4936)
                     /locus_tag="PK171_02470"
     CDS             complement(4064..4936)
                     /locus_tag="PK171_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05100.1"
                     /translation="MNSGRKVLRMIGIYVGAIIVVVVVLFPYLWMVSGSFKSTLEIQS
                     ADFRDPELAPRWIPRHFTWENYIRINQTVRMLDYFRNSMIISGGTMLASILLSLFAAY
                     SLSRFRFRLKEPYVGSLLLTQMFPGILFLIPYYVMFVVFNRFTGVQLRNSYLGMIFTY
                     TSFALPFSILMLRSFLAGIPREIDEQAQIDGCSTLGIIFRIILPLARPGIAAVAIYGF
                     IMAWNEMLFASVLTGTETKTVAIGLLEFITTQESRWAGMMAASVIVTIPVLILFTFVQ
                     KQIIEGLVGGATKG"
     gene            complement(4943..>5660)
                     /locus_tag="PK171_02475"
     CDS             complement(4943..>5660)
                     /locus_tag="PK171_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05101.1"
                     /translation="FDQQEGQNFLRSLYNIVLYGGITISLGYVIALAVAILLNQKFFG
                     RTTVRGLVLLPYIMPDSVAYSVWRFIFQSRIGIVNQYLISLGLLKEPVVWLVGPRAMT
                     AVIIASIWKGWPLGCLLLLAALQSVPREIKDAALSDGATPWQVFRYVTFPFLKPVSKI
                     YILLSIIWNFHAFNQFKAMLGSSPGYYAEVPSTLILREAFQNFHFGLGAAMSMGLVVL
                     TVVIAVVYARLFQPGKTIEE"
BASE COUNT         1746 a         1271 c         1078 g         1565 t
ORIGIN      
        1 ggtagttatt atgttatatg aggtgtcttg atttgtcaaa tttcgagagg agagtgaaag
       61 ggtagtggtt gagctttttt aaaatcaggt agtcttcaat tttattgaaa tgataaggaa
      121 aatttaaaaa aagaaaaatt cttaaaaaat ttaaagaact tttctgcttt cgccctctcg
      181 cattctctcc ctcactctat ctgtctttta atcttctcct aaaataactt ctaccagatg
      241 ttcgctccct ggagaaaaaa ttggaatagt tttacccgaa attgtttttc catctacttt
      301 taataaatac ttctcgttcg gttttctctc aaccacaatg gtaaattgaa caccacgaaa
      361 ccaacgctgt acctgaaaac ctttccaatc agcaggaata caagggtcaa cgattaaacc
      421 ttgataatcc ggacgaatcc ctaatatcca ctgagtagct gatatcaaag cccaggaagc
      481 ggttccggtc aaccaagaat tttttgcttc accaaagtgt ggatggtcag gtccagctat
      541 catctgggca taaacatagg gttcctgtcg gtgtacttca gaaactttct ctctccaaga
      601 aggacatata gttaaccaat aattccaggc tttttcagct cttttcaatt tacactcagc
      661 tattactgcc caaggatttg gatggcaaaa aattccggca ttttccttta aaccaggcgg
      721 ataggatgta cattctccta aatgtaaaga aaactgagtg aaagccggtt gctggagaat
      781 aattccgtga ggggtagcca gatatttttc gaccgaatcc atagtaacct tccctcgttc
      841 cagattagca acaccactca taattgccca aggttgagtc tctaaaaaaa ttctcccttc
      901 ctggttttgc tttgagccca caggcaaccc tctatcatca aaagctctca agtaccattc
      961 accatcccaa ccccattggt ttattttatc aatcattttc ctggtttctt ctcgaaatcg
     1021 gatagaatct tctgatttac cgattatctc agcaatacaa gccagttctt ctccagctgc
     1081 aataaacatc tctccaatga gaattgattc agcagttttt ccttcatttt cggtaactaa
     1141 atttaagtta agacaatcat tccagtcggc ttgtccgatc aaaggcaaac catgaggtcc
     1201 aagattgtga agagtatatt ccagagatcg gagcaaatgt tgataaaaag gtgcaggatc
     1261 cccaccatca gcaaaggaaa ccacctcatt taaaattgta aaatctccgg tttctttgag
     1321 ataactagcg gcagccaaaa ttaaccaaag aggatcatcg ttaaaaccag ctccaatttc
     1381 atcattcccc tgtcgggtaa gtggttggta ttggtgatag gcacctcccg attgaaattg
     1441 agtggatgac aaatccagca aacgttctct ggctctatcg gggataagat gtacaaatcc
     1501 caacaggtct tgattgctat ctcgaaaccc tattccccgg ctaattcccg attcaaaata
     1561 agaagccgag cgggatagat tgaaggtaat catgcattgg tattggttcc aaacattgat
     1621 cattcgatta aaatgggtat tgggtgtttg aacctggaaa cgttcaagaa tatttttcca
     1681 atacgatttg agctttccca aagcccggtc tacctcgtta tgattggtga aacgttgtag
     1741 aagagacggg atcccctcct tattcacccg tctctcggca gtgaatttat gctgtgttag
     1801 gttttcctta taccccaaca gaaattgaat acgctcctcg tgttgagagg gaagggttac
     1861 ctttatctga tgaactccaa caggcgccca accacaggca attgagttgg aggtttttcc
     1921 ttcccttact gaataagggg agctcaaaga ttcgtaagga ccaagaaaac tctcccgatc
     1981 cgtctcgaaa cctacaatat ttcccaccgc accaaaaaaa gcataatggt ctcgtctttc
     2041 tcgatattcg gtgatatggt aaattactcc ttcttcaacc tcaacttctc ctatattaag
     2101 gttgcgttga aagtttgtag catcgtcaag agcattccat aaacagaact cgacaaagga
     2161 aaaaacatca agttccagtg tttcctttcc ccggttaaag agtttaaccg accaaatctc
     2221 aagttcttct ccaattggaa cgaaataggt tatctcgctt tcgatatctc gataaagaga
     2281 agagatgata gtataaccca acccgtgccg acaacggtaa aaatccaact tttttcttac
     2341 cggttgccag gtaggagaga agaaatcccc atcagaaaaa cggatatata aataacgacc
     2401 tcctttgtct tgaggaatgt tgttatagcg gtagcgtaga atccggcgga ggcgagcatc
     2461 tcgataaaaa ctgtatccac cggcagtatt agaaataata ccgaaatatg actcctcccc
     2521 taaataatta atccaaggtt ggggggtgtc cggtcgttcg ataacatact ctttattttg
     2581 atcgtcaaaa tatccataaa gcaaggattt cttcctcctt tttcattacc tggataacct
     2641 taccttccgt taacaaagtg tcatgataat caatcccggg ctaatgctcg attcacccta
     2701 tcagcagcag tatcaagata ttcttgaact ttttcatcag aataagtttt tgcaatataa
     2761 gcggttagaa tatttcgaaa ttcttcttga acggcatttt caacatcgcc ccatctggct
     2821 aaaggtggat aagcagtagc ataggatagg gttgtttgaa aaactttcca gactcctgtt
     2881 tcataatagg gatcatcata tgcttccaaa agggcaggta acatttgaga aagattttga
     2941 gtgtaatcaa ccagcttttg tttttccaaa aggaatttga tccatttctt tgctgcttct
     3001 tgattggaac tggctttgag aataactaaa ttgcttcccc ctgagaaaga agcccgacct
     3061 gcaggtccag caggaggttc tacaacatca tagttgaggt cagggttttc tacttcaata
     3121 ttggcaatat tccagggacc catgaaacac attgctacgt tcccattgat gaaagctgaa
     3181 tcagcttggg gttggttgta ctcagcacac gcttcatctg cataaccatt tgccaccagg
     3241 ccaacatacc atttcatagc ttccacacca ggagcttcat taaaacgggc ggtcttggtt
     3301 tgttcatcaa ccaacactcc tccatgagcc caaagaacga tcgcccagtt atgaagaaga
     3361 tcccaagcat tggttccagc aattgaaatt gcccggcctt tcccaaattt ttcgttaatg
     3421 cgttcaccag cttcgtagag ttcctgatag gtctgaggtg gttccatccc agcttgctga
     3481 aacatatctt tgttataaaa taatgctcgg gtttcagcaa accaagggac accaaaggtt
     3541 ttttcttgat aggtagtgga tatatagttg gctttcataa aagcaccggc tccaccaaat
     3601 tcattgatgt caatttcagt taatcctccc gttgctgcaa attgaggatt ccaagttgtt
     3661 ccaacttgga aaacatccac tccctcacca gtagcgatag cagaggtgat tcgcgtccag
     3721 gcatctcccc aaccaacgat ttcataggta accgagatac cggtttgttc tttgaattca
     3781 gcgacagctt cgttcaacca agggacatgg atatcgtccg gtgcattggg catgagccaa
     3841 aagttgatgg attcagctgc ctgtaccgct atcgacacac aaaagaccat tattaaagac
     3901 cataagaaaa tatttttaaa catcttcctt cctcctttaa attataagct tataaactat
     3961 ttaaacctta tcaactccaa cgattatccg cttttttttc caatctctaa aaaccacctc
     4021 ctttataagt aaaagagact tgtctttccc cctacatcca aaattatcct ttggttgctc
     4081 ctcccactaa accttcaata atctgttttt gtacgaaagt aaatagaatg agaaccggta
     4141 tggtcacaat gactgaagca gccatcatcc cagcccagcg agattcctgg gtggttatga
     4201 attctaagag accgatagca actgttttgg tctcagtccc agtgagaacc gaggcaaaga
     4261 gcatctcatt ccaggccata atgaaaccat atatcgctac agcagcaatc cctggtctag
     4321 ctaatggaag tataatccga aagataatgc ccagggtgct acaaccatcg atttgagcct
     4381 gctcatcaat ttctcttggt ataccggcta aaaagctgcg aagcatgaga atactgaaag
     4441 gtaaagcaaa ggaagtgtat gtgaaaatca ttcctagata ggaatttcgc agttgaacac
     4501 cggtaaatcg attgaaaacg acaaacatga cataataagg aatgagaaaa aggataccgg
     4561 gaaacatttg ggtcaacagc aaagatccaa cgtatggttc tttcaatcgg aagcgaaatc
     4621 gagataaact ataagcagca aatagactca aaagtatcga agccagcatc gtacctccac
     4681 taatgatcat gctgtttcta aaataatcaa gcatcctgac cgtctgattg attcggatgt
     4741 aattctccca agtaaaatga cgaggaatcc agcgaggtgc caactctgga tcacgaaagt
     4801 cagccgactg gatctctaaa gtgcttttga atgaacctga gaccatccac aaataaggaa
     4861 aaagaacaac cactactaca atgattgctc caacataaat tcctatcatc ctaagtactt
     4921 ttcgtccaga attcatggct ttttactcct caatcgtctt accgggttgg aagaggcggg
     4981 cataaacaac ggcaatcact accgtcaaaa caaccagtcc cattgacatg gctgctccca
     5041 aaccaaaatg aaaattttga aaagcttcgc gtaagatgag ggtagaaggt acttcggcat
     5101 aatatcctgg gcttgatccc agcatagctt taaattgatt gaaagcatga aagttccaaa
     5161 taatactgag tagaatataa attttactaa ccggtttcaa aaacggaaag gtgacatatc
     5221 ggaagacctg ccagggagtc gctccgtcag agagagcagc gtctttaatt tcacggggga
     5281 cgctttgcag tgcggcaaga agtaataaac accctaaggg ccaacccttc caaatactcg
     5341 ctataatcac agcagtcata gctcgaggac ccactaacca cactactggt tcttttaata
     5401 aaccaagcga aataaggtat tgattgacga tgccaatccg actttggaaa atgaaccgcc
     5461 aaacagaata ggcaaccgaa tcaggcataa tatagggaag gagaaccaga cctcttactg
     5521 ttgttcgacc gaagaatttt tgattcagca aaatcgcaac cgccagggcg ataacataac
     5581 ccaggctgat agtaataccc ccgtataaaa caatattata aagggaacgg agaaaattct
     5641 gcccttcctg ttgatcgaag
//
LOCUS       DAOSOL010000063         5654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14458_length_5654_cov_3.469905, whole genome shotgun sequence.
ACCESSION   DAOSOL010000063 DAOSOL010000000
VERSION     DAOSOL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14458_length_5654_cov_3.469905"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(627..806)
                     /locus_tag="PK171_02480"
     CDS             complement(627..806)
                     /locus_tag="PK171_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05102.1"
                     /translation="MKLENKVFKTKGEALSNAIAMNLIDFEVITDRDNTGKIVYRIDL
                     IQNDCSINSYNFEYL"
     gene            complement(1144..2268)
                     /locus_tag="PK171_02485"
     CDS             complement(1144..2268)
                     /locus_tag="PK171_02485"
                     /inference="COORDINATES: protein motif:HMM:NF014428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT05103.1"
                     /translation="MSRRKILLTGFLVVASVINLFMIPTGFAQPKFTLNSDIYLAIPQ
                     SHSVNISIDRGQDSTYFIDDPIIIRYGATKTGYINIFDYNPGGSVLLLVRNQEIIAGS
                     NLILNSKVKGPTGIDRLIILFTPKPVGENELKIFIESPHQSNRYFPLSAVNRIHFKVA
                     DRNPRAPNITLEPSQFIIEPGSCYSITAQLNYHNGQPVQNSPVDWSTDYGTLNTKSNL
                     TDNNGKATVTYQAPESNQIKTAVITAFSPGHAGIPPISVKAVVNIVCRTHSTEIIVCP
                     SSFSTQSGDKIQFTATLQDISGNPICDRTLYWSSNVGDLNESSAITNLSGQVTFSFYT
                     PEVNEGTSVNLTVEFRGAEGLCPSKITLLGNIKPVSSTNP"
     gene            complement(2986..3330)
                     /locus_tag="PK171_02490"
     CDS             complement(2986..3330)
                     /locus_tag="PK171_02490"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOT05104.1"
                     /translation="MRNSILLFLLIFSFFGFIPQEVFANEISSPGWGWSHMMPWGWMN
                     WGWGGMIGIGLIGLIVVLALIYLVFQNTRFSSDSGRRTSDDRPLEILQKRYAKGELSR
                     DEYQKMKEDLKE"
     gene            complement(3626..>5654)
                     /locus_tag="PK171_02495"
     CDS             complement(3626..>5654)
                     /locus_tag="PK171_02495"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOT05105.1"
                     /translation="IESSNNNKLSQSSPSHHTGPNLHTGHNVEMFRNKFWVCLIISIP
                     VLLWDPMIQQWFKYQLPSFPGSNFIPAVFGTIVFIYGGWIFLQGAYQELVHKTPGMMT
                     LISLAINVAFFYSIAVMLGLKGHTLWWELATLISIMLLGHWIEMRSISQAQGALQELA
                     KLLPDTAILLTDDESTQEVLVEQLQEGDLILIRPGASIPADGMVQSGKSSVNEAMITG
                     ESKPVEKKQGERVLAGTVNGEGSLRVKIAGIGDKTALAGIMRLVEQAQSSRSRAQALA
                     DRAAFYLTLVAIGSGLITLIGWLLSGSTLDFAITRVVTVLVIACPHALGLAIPLVIAI
                     STTLGARNGLLIRDRRGLEEARNLDVVVFDKTGTLTMGEHRVIQTVTDKSIDNEEALR
                     LAAAVERDSEHPIARALQKSAEAVGIEIPPSQNFRAIPGQGVQAQVEGREFQVGGPSL
                     LKSEKFPIPEEFNDAAHRFGESGQAAIYLVEGEKVLAVFAIADAIRPESYEAIRNLHQ
                     LGIQSAMLTGDSEAVAETVAKELDINLVFAQVLPEDKAKKIQDLQKQGKRVAMVGDGV
                     NDAPALATADVSIAIGAGTDVAVEAGDVVLVRSDPRDIPRIIKLSKATYQKMIQNLWW
                     AAGYNIIALPLAAGALAWAGIILAPAVGAVLMSASTVIVSINAQLLRRVQL"
BASE COUNT         1745 a         1088 c         1112 g         1709 t
ORIGIN      
        1 ccctccccgc cgcaaagcgg cacccctcca aggaggggaa ttgttgaatt cattctttct
       61 catttttctg tttgattact ttcaaccatt catgtattta tattatggaa gctcatacta
      121 ctaagtagca tcagatgaga ttaaaattgt tgccccctca cttacgcctc tcccacatgg
      181 tgagaaaaaa aagaaaaatg gaagatggtt gaaataaggg tgaaaatact ttgtagggct
      241 tgatttatca tggccacagt cgaattatca gaataggata aagggtatgg atgttttaat
      301 tgtggtaaaa ataccttgag gtgccccaaa ataaatttct tttaggaaat tattggggta
      361 aattattaag aaaataagga ataagaaagt aatagtgata aaaatattgg ttagaatagc
      421 tctttttgat aaatttattt aatgtaattt caagaaaaat actcttagat atcagactta
      481 ttatcagcaa tgaaacctta agtaagtttg ttggccttaa gtctcagttt ggctaataag
      541 ttgaagagat agcaaaactc agttcttaag gacttaatga atttaccgaa gaatgaaaaa
      601 tctcttaaat tgattagaaa attgatttat aaatattcaa agttatagct attaatagag
      661 caatcatttt gaataagatc aatacgataa actatctttc cggtattgtc tctatctgta
      721 atcacttcaa aatcaatgag attcattgca atagcatttg acaaggcttc ccctttagtt
      781 ttaaaaacct tattttctaa tttcattgcc cttcccccct ccttattata tagattaact
      841 aaatgagata atatttctat aggaaagaat tcccattttt tgttcctaag agactatttt
      901 ttgaaatcat atatgaggaa taaggcctaa aagaaatgga aaaaatgtag gttaagaatt
      961 tatttggtta ttcaaacacg aaaatagaat gagaagcgag tggtgagaag atataatcac
     1021 ccttattttc accttctccc gtcaacagag aaagaaccta aaaccgtctt gacgaggaac
     1081 ctagcgacct ggcaatctct tcagttcaac cattaatatt tatctctctt tggttaaaaa
     1141 agcttaagga ttggttgagg aaaccggttt tatatttcct aaaagggtaa tttttgatgg
     1201 gcataggcct tctgcccccc gaaattctac cgttaaatta acagaagttc cttcattaac
     1261 ttcaggagtg taaaaagaaa acgtaacttg tccagagaga ttggttattg ctgatgattc
     1321 gttcaaatca ccgacatttg aggaccaata aagagtcctg tcacagattg gattaccact
     1381 gatgtcttgt aaggttgcgg taaattgaat tttatcacca gattgagttg aaaaactcga
     1441 aggacaaacg atgatttcag ttgaatgagt ccgacataca atattcacca cagctttgac
     1501 acttatgggg ggtattccag catgtccagg tgaaaatgca gttatgacag cggtttttat
     1561 ttggtttgat tcgggagctt ggtaagtgac tgtagcttta ccgttattat ctgttagatt
     1621 agatttggta ttcaacgttc catagtcggt tgaccaatct acgggactat tttgaactgg
     1681 ttggccgttg tgatagttga gttgggcggt tatggaataa caagaacctg gctcaatgat
     1741 aaattgagaa ggttcaagag ttatatttgg agctcgtgga ttccgatcgg caaccttgaa
     1801 atggatacgg tttacggctg aaagcggaaa ataccggtta ctctggtgag gtgattcgat
     1861 gaatattttt aattcatttt ccccaactgg ttttggagtg aaaaggatta tcaatcgatc
     1921 aatacccgtt ggccctttta ctttgctatt taatattaaa tttgaaccag caatgatttc
     1981 ctggtttctc actaataata aaacacttcc accgggatta taatcaaaaa tattaatata
     2041 acctgttttt gttgctccat agcgaattat gattggatca tcaatgaaat aagtagaatc
     2101 ctgcccacga tcaatcgata tatttaccga gtgtgattga ggaatagcaa ggtatatatc
     2161 agaatttaag gtaaattttg gttgagcaaa gcctgtagga atcataaata aattaattac
     2221 cgatgccaca accaaaaaac cagtgagtaa aatttttctt ctactcattg gattcctcct
     2281 tactcttcaa aattgactat agtaaacagt ggtacgaaag tgccttaatt attctctatg
     2341 gaaaaattgt ctttatcgaa aaaacaccat aaaaaaacta tactgaaaat tgttttcttg
     2401 tttagggatc ttccctttca ttattttaac tcaatagctt aacaatagaa aattcctttt
     2461 ctcagatcct ggtttatggt ataggggaag aaagggaagt attggcgaaa gacgacgagg
     2521 gggtgagggg cagaaggggc gacttcttgg gtttcttctc gcttgatggg agaaggtcag
     2581 gatgagggtg aaaacctttt gtaggggttt gatttatcat gcccatagat aggtttttca
     2641 agatagtcaa aaagacacct ctctcttttt ggtgattgtt taattgctaa agtaaatttt
     2701 accagaaaga tagggcatat tcattaaaaa aattttcata ttaatgtatt tcagtattta
     2761 ttttaatatg aaaattggag gaaaaagcaa aaatggataa tgaaaaaaaa caataaaaaa
     2821 aaccaaccac gtaaaaaata ataaaatatc atcattgtct ggcactcagc tattaaatta
     2881 ttctttgcag ctgacaaact tataatctaa ttggttttga ctagaaacaa taaagtttat
     2941 cttgtaaata aaaacctgac tcccagtaag gagtcaggtt tttatttact ctttcaagtc
     3001 ctctttcatc ttctggtatt catcacgcga caattctccc ttggcatagc gtttttgaag
     3061 aatctccaag ggtctatcat cacttgttct ccgaccagaa tcagacgaaa aacgagtatt
     3121 ctgaaatacc agataaataa gggctaggac cacgatcaaa cctattagtc ctattccaat
     3181 cataccaccc cacccccaat tcatccaacc ccagggcatc atgtgcgacc agccccagcc
     3241 aggactacta atctcatttg caaaaacttc ttgaggaata aacccaaaaa agctaaatat
     3301 gagtaaaaac aacagaattg aattccgcat tgttttcacc ctctttcttt tcaatgtaaa
     3361 taaattttaa ttattatgat taaagagcca cttagtttaa tttttcctaa tatttcagat
     3421 tttcagtctt ttatgcttaa tgttcctttg ctgagaggac tgcgaacgtg aattatttct
     3481 cctcattagt gcgtgactga atttaaacat tagtaattat attttaataa tataaaacat
     3541 aaattcaatt ggtattattc cattatggat aaacaccgag attctttttt ctagattctg
     3601 tgtggaatga atgagggtaa atatattaaa gctgaacgcg tcgtaaaagt tgggcgttaa
     3661 tagaaacaat gaccgtactc gctgacataa ggactgcacc aaccgctgga gccaggataa
     3721 tgcctgccca ggctaaggct cctgctgcaa gagggagggc gataatatta taaccggctg
     3781 cccaccagag attttgaatc atcttctggt aggtcgcttt actaagtttt ataattcggg
     3841 gaatatcacg tggatcagaa cggaccagca ccacatcacc ggcttcgaca gcgacgtcag
     3901 ttccagcacc gatagcaatg ctgacatcag ctgtagccag agcaggagca tcgtttactc
     3961 catctcccac catggcaact cgtttacctt gtttttgcaa gtcttggatt ttcttggctt
     4021 tgtcttcagg gaggacttgg gcaaaaacta agttaatatc taattccttg gccacggttt
     4081 cggcaaccgc ctcggaatct ccggtcagca tcgctgattg aatacccaat tggtgtagat
     4141 ttcgaatagc ctcgtacgat tcgggacgaa tagcatcggc tatggcaaat accgcaagaa
     4201 ctttttcacc ttcgaccaag taaattgccg cttgaccaga ttcaccgaaa cgatgagctg
     4261 catcattgaa ttcttcggga ataggaaatt tttcactctt taaaagagac ggtcctccaa
     4321 cctggaattc acgaccttct acttgagctt gtaccccctg tcctgggatg gctcggaagt
     4381 tttgggaagg tggtatttca attccaactg cttcggcact tttttgaaga gctctggcta
     4441 tgggatgttc ggaatctctc tcgaccgctg ccgctaggcg cagagcttct tcgttgtcta
     4501 tacttttgtc ggtcacggtt tgaattaccc gatgttcacc catggttaaa gtaccggttt
     4561 tgtcgaatac tacaacatca agatttcttg cttcttctaa tcctcggcga tcccggatca
     4621 acaggccatt acgagctccc agagttgtgg aaatagcgat aaccaacggg atagctaatc
     4681 ccaaggcgtg gggacaagca ataaccaata cagttaccac gcgagttata gcgaaatcaa
     4741 gagtggatcc agataatagc cagccgatta aggtgatcaa acctgaacca atagccacca
     4801 aggtaagata gaaagctgct cgatcagcta atgcctgagc gcgggaacgt gaagattgcg
     4861 cctgttcaac tagccgcata attccggcga gggctgtttt gtcaccaatt ccggcaatct
     4921 tcacccggag agaaccttca ccgtttaccg taccagcgag gaccctctcg ccttgctttt
     4981 tttcgaccgg tttcgattcg ccggtaatca tggcctcgtt gaccgaactc ttgccacttt
     5041 gcaccattcc gtcggcggga atgctcgctc cagggcggat taaaattaag tctccttctt
     5101 gcaattgctc aactagtacc tcttgggtcg attcatcatc agtgagaagg attgctgtat
     5161 ccggtaatag cttggctaat tcctggagtg ctccctgagc ttgagatatt gacctcatct
     5221 caatccaatg cccgaggagc atgattgaga tcagtgttgc caattcccac cagagagtat
     5281 ggcctttcaa tcccaacatc actgctatgc tgtaaaaaaa cgcgacatta attgccagcg
     5341 agataagggt catcataccc ggagttttat gaacaagctc ttgataggct ccctgtagga
     5401 aaatccatcc accataaatg aaaacgatag ttccaaaaac tgcagggatg aaattggaac
     5461 cagggaagct aggtaactga tatttaaacc attgctgaat cattggatcc caaagaagca
     5521 ccggtatgga aatgattaga catacccaga atttgttcct gaacatttca acattatgac
     5581 cagtatgcag gttaggaccg gtgtgatgag aaggggatga ttggcttaat ttattattat
     5641 ttgacgattc tatt
//
LOCUS       DAOSOL010000064         5639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14500_length_5639_cov_4.259850, whole genome shotgun sequence.
ACCESSION   DAOSOL010000064 DAOSOL010000000
VERSION     DAOSOL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14500_length_5639_cov_4.259850"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PK171_02500"
     CDS             complement(<1..734)
                     /locus_tag="PK171_02500"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT05106.1"
                     /translation="MKAAHFLGPGQMEVREVPKPEIGDGEVLLKVAACAICGTDVRIF
                     QFGHPKVKPPHITGHEISAEIVEVGKGVEGYKIGDKVSVITVISCGRCHYCRRGLQNL
                     CPDQEYIGYHFPGGFAEYLKIPKAGVDKGNLLVLPPDMDLLEASLVEPLSCAVNGQSY
                     LNIGFGETVLVIGSGPIGCMHVELAKNRGAGKIIIADISGERLNLAKRVQADYYINSA
                     EKNLKDEVLTITDGLGADVIIVAASSG"
     gene            complement(1012..2523)
                     /locus_tag="PK171_02505"
     CDS             complement(1012..2523)
                     /locus_tag="PK171_02505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT05107.1"
                     /translation="MDLSYLGIDVGTTGCKVIAFDEEGRTVAQAYREYPLYQLNQGWI
                     ELDADEVFRSVEDCLQEVSNFYRGDYPVSLAISAQGEAVIPVDEKGGCLARSMVTFDE
                     RGDEFVPFWKEKLGEEKFFKITGMPLSGIGTINKILWWKKYTPDIFSKARYFLCYEDF
                     IVFRLGLKPAMNYSLAGRTMMFDVKKAQWSSEILDIAGIDENQLAQPFPSGQAIGLVS
                     EAFRKKMGWPHPVLVACGAHDQPSGALGAGVIRPYLAMDATGTVECIAPAISQLVLTP
                     LMRENNLCCYHHSVSDLFITLVYNFTGGQLLRWYRDNFAGLEKEQSRLLQKNVYDLIL
                     DGLPEDPTNLYVLPHFTTTGTPYFDTNSCGMIVGLKFQTTNKEVVKALLEGISLEMKF
                     NLELLSNAGVEVRALRAIGGGARNEHWLQIKADVYNLPVETLNVSEAACLGAALLGRK
                     ARENIEDFSSLVNSIVQVKQVYEPNPTNANRYLEKYEVYKHLYPALKQCIYKN"
     gene            complement(2565..3536)
                     /locus_tag="PK171_02510"
     CDS             complement(2565..3536)
                     /locus_tag="PK171_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT05108.1"
                     /translation="MKSKGFSTGIWVFGATPDRFCVTGYKSGGNIEESLDRIAAVPNL
                     TGVMMHYPQPLNENNVDFVKKALQDRGLKLACCDVDLFSDPIFARGSLMSEDSELRKK
                     AIDYSKKAMDIAEYLEAGVMNLWPGQDGFDYPFQINYVAQWNLLVEALIEIAQHNPRV
                     RLSLEYKLREPRTHSTIYSSGTALYLSHATGCSNVGVTVDLGHSFNCKESPANVVALL
                     DKFEKLFVLHLNDNYRDWDDDMAVGSVHFWETLEFIYYLNLSSYEGWLGLDIFPYRED
                     SAEACRFSIENIQYMLEVVNKIDLKTLHQIQKEANSLEAMRYIGSLL"
     gene            complement(3933..5465)
                     /locus_tag="PK171_02515"
     CDS             complement(3933..5465)
                     /locus_tag="PK171_02515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT05109.1"
                     /translation="MVLAGLDVGTSGCKVVLFREDGTMVAQSYREYPFITPQPGWLEI
                     NPEHVWESVLDALNEATKKSKEPIEVVAVTSHGETLIPIDRSGKALYNALANFDTRAH
                     TYIDYWLERENPYNIFRITGMPLHGMYTVNKILWLKDHCPDVFKKTWKFCCVEDYIIY
                     RLTEDQPVIDYSLAARTMLFDVVKKEWSGKICKLAGVDPQNLSRAIPSGSIAGTISSK
                     VSGISGLPKNLLIATGGHDQPCGVLGCGVKNPGEAMYGIGTSECVALNLGGSAFFSRE
                     MLENSFCCYPHVVKDSYITLAYIASGAVVLRWFRDEFGFEELEKAAQIGGSAYDLLLK
                     EIPDKPASVFVLPHFSGTGTPYLDEKSRGAIIGLTLATSKEEVVKAILESLTYEMKIN
                     LDLYERFGLPINSLRVIGGGSRSRAWLQIKADILQKALIIPKTGEAVALGTAMLAGVA
                     KGVFKSFEEAIDAMVSFEDSIQPNNSYLQNYNNRYQIYQLIYKDLLKVNRMISDLTRE
                     EQ"
BASE COUNT         1676 a         1286 c          970 g         1707 t
ORIGIN      
        1 ccagatgatg ctgctacgat gataacatcc gctcccaaac catcggtaat agtcaacact
       61 tcatctttta ggttcttttc ggctgagttt atatagtaat cggcttgtac tcgtttagct
      121 aagttcaaac gttccccgga tatatcagca atgatgatct taccggctcc tcggtttttg
      181 gctaattcaa catgcataca tccgatagga cccgatccga tcactaaaac agtttctcca
      241 aatccaatgt ttaaatagga ttgtccattt acagcacagg aaagcggttc taccaagctg
      301 gcttcaagca aatccatatc tggcggcaaa acgagtaaat tgcctttatc aacgcctgct
      361 tttggtattt ttaaatactc ggcaaacccc cctggaaaat ggtacccgat gtattcttgg
      421 tcgggacata gattttggag accacgtcgg caataatgac agcgtccaca ggaaataact
      481 gttatcaccg aaactttatc tccaatctta taaccctcta ctccctttcc tacctcaact
      541 atttcagccg atatttcatg accagtaata tgaggcggct tcacttttgg gtgaccaaat
      601 tggaaaatac ggacatcggt tccacagata gcacaggcag ctacctttaa caacacttca
      661 ccgtcaccaa tttctggctt cgggacttca cgaacttcca tttgacctgg tccgagaaaa
      721 tgcgcagctt tcattttatt ttctctcctt tctatttcta aaggcagtga tgtgtgacac
      781 ataatcaatt ccaattttta cccccatact tatcttctct cttcaagaga gaaggaattg
      841 ttgtattacc aatgggcagg gtcgtttatc ctgctatcgt gaattggaac ctgtgacaag
      901 attaaaccat cccaaaataa gtttattctt gtgaacttac tccatctacc aaaccggaaa
      961 actatatttt tttacttttc catctccatt atcaatacca atcacttttc tttaattttt
     1021 ataaatacat tgtttaagag ctggatataa gtgtttataa acttcatatt tttcaaggta
     1081 ccgattggcg ttggttggat ttggttcata tacttgtttt acctggacaa tcgagttaac
     1141 taatgaagaa aaatcctcaa tattttctct ggcttttctt cctaataaag ccgctcctaa
     1201 gcaagcagct tcggaaacat tcagggtttc aactggcagg ttatatacat cagctttaat
     1261 ctgtaaccaa tgttcatttc gtgctcctcc accaatcgca cgtaaagctc tcacctcaac
     1321 tccagcattt gaaagtaact caagattaaa tttcatctcc aagctaattc cttctaaaag
     1381 agctttcacc acttctttat tggtagtttg gaattttaat ccaacaatca tcccgcaaga
     1441 attcgtatca aaatatgggg taccagtggt ggtaaaatga gggaggacat aaaggttggt
     1501 tggatcttct ggtagtccat caagaatgag gtcataaaca tttttttgaa ggagtcgtga
     1561 ttgttctttt tccaagccag cgaaattatc ccgataccag cggagaagct gcccgccggt
     1621 aaaattataa actaaagtga taaaaagatc ggaaaccgaa tgatgataac aacaaagatt
     1681 gttttctctc atcaaaggtg ttaacactaa ttgagagata gccggtgcta tacactccac
     1741 ggttccggtg gcatccattg ctaaataagg tcgaatgacg cctgctccca aagctcctga
     1801 tggttggtcg tgagcgccac aagccactaa gactggatgt ggccatccca tcttttttct
     1861 gaaggcctca gaaacaagac ctattgcttg ccctgatgga aagggttggg ctaattgatt
     1921 ctcgtcaatc cctgctatat ctaaaatttc tgacgaccat tgagcttttt ttacatcaaa
     1981 catcatagtt cgacccgcta aagaataatt catggctggt tttaatccca atcggaatac
     2041 aataaaatct tcgtagcaaa gaaaatagcg ggcttttgaa aatatatcgg gagtatactt
     2101 tttccaccaa agaattttat taatcgttcc aatccccgag agaggcattc cggtaatttt
     2161 aaaaaatttc tcttctccca gcttttcctt ccagaatgga acgaactcat cacctcgctc
     2221 gtcaaaggtt accatgcttc ttgctaggca accacctttt tcatcaaccg gaatgaccgc
     2281 ttcgccttgt gcagatatag ctaaacttac tgggtaatca cctcgataga aattggatac
     2341 ctcctgtaaa caatcttcaa cggaacgaaa aacttcgtca gcatccaatt cgatccatcc
     2401 ctgattcagt tgatataagg ggtattctct ataggcttgg gctactgtac gaccctcttc
     2461 atcaaaagcg atcactttgc agccagttgt tccaacatcg atacccaaat aactcaaatc
     2521 cactcacctc tcctatttag aaaattcaat tcaccaattc ttatttataa taagcttcct
     2581 atgtaacgca ttgcttcaag ggaatttgct tctttttgaa tttggtgaag cgtcttgaga
     2641 tcaatcttat taactacctc taacatatat tgaatatttt caatactaaa gcggcaggct
     2701 tcagcagaat cctctcgata agggaaaatg tctaaaccca accatccttc ataactggat
     2761 aaattcaggt aataaatgaa ctcgagggtt tcccagaaat gaactgatcc aaccgccata
     2821 tcatcatccc aatcccgata attatcatta agatgaagaa caaataattt ttcaaattta
     2881 tccaaaagag caaccacatt agccggtgat tctttacagt tgaaagaatg acctaaatca
     2941 accgttaccc ctacattgga acatccagtg gcatgagaaa gatagagggc tgttcccgaa
     3001 gaataaattg ttgaatgggt ccgaggttca cgaagcttat attcaaggct aagacgtact
     3061 cgaggattat gttgggcaat ttcaatcaat gcttcaacta ataaattcca ttgtgcaaca
     3121 taattaatct gaaagggata atcaaaacca tcctgacctg gccagagatt cattacaccc
     3181 gcttccagat actcagcaat atccattgct tttttggaat aatcgattgc cttttttctt
     3241 aattcggaat cttctgacat cagacttccc cgggcaaaaa ttggatcact aaacaaatcc
     3301 acatcacaac aggctaattt taagccccga tcttggagtg cttttttaac aaaatcgacg
     3361 ttattttcat taagtggttg aggataatgc atcattacgc cagtgagatt aggaaccgca
     3421 gcgattcgat ctaatgactc ttcgatgttc ccacctgatt tgtatccagt tacacaaaac
     3481 cgatcggggg tcgccccaaa aacccatata ccggtggaaa atcctttact tttcattgac
     3541 tttatcctcc ttttgtttct atcctgataa tataaaataa ttttatgtta ccatcatcct
     3601 aagcggtgct ttcccgcgtg aggatcccat ctggcgccac cggcgtcatc ctgaggcttc
     3661 gctttatgaa gccgtgagga tctcatcatt ttatcttttt ctttaaaaaa tttttactaa
     3721 atgagattgc cacgtcgccg agctcctcgc aatgacggag cagtaaaaaa ttcaaatctc
     3781 ccttaccccc tttgctaaag ggggaaattg atcggcgtat atatagtttc atcctcatct
     3841 ggtgtcatca aagtggcatg agggtctaat aactatttta aaaaacctgg taatattaat
     3901 ctcctttttt attaaaaatg gggataaaat aactactgtt cttctcgggt cagatcactg
     3961 atcattctgt tgacttttaa caaatcttta tatattaact gatatatctg ataacgatta
     4021 ttatagtttt gtaaatagct attgtttggc tggatggaat cctcaaagga aaccatggca
     4081 tcaattgcct cttcaaaact tttgaatact cccttggcaa ctcctgccaa catagcagtt
     4141 ccaagagcaa ctgcttcacc agtttttgga atgatcaagg ctttctgaag aatgtcagct
     4201 ttaatctgaa gccacgctct cgaacgagaa ccaccaccaa tcactcgcaa gctgttgata
     4261 ggtaaaccga acctttcata taaatctaaa tttattttca tctcataagt gaggctttcc
     4321 aaaatcgctt ttactacttc ttccttgctg gttgctaaag taaggccaat tattgcccct
     4381 cgtgactttt catcaaggta tggtgttccc gtgccggaaa aatgcggaag aacaaatact
     4441 gaggcaggct tatccggtat ttcctttaat aatagatcgt atgcgctccc accaatttgg
     4501 gctgcttttt caagttcctc aaatccaaac tcatctcgaa accaacgaag cacaaccgca
     4561 cctgacgcta tatacgccag agtaatgtag gaatctttca caacatgagg ataacagcaa
     4621 aaactgtttt ccaacatttc tctgctaaaa aaggcgcttc ccccaagatt gagcgcaacg
     4681 cattcggagg ttcctattcc atacatcgct tcacctgggt ttttcactcc acatcccaaa
     4741 actccacaag gctggtcatg gcctccagtg gcaattaaaa gattttttgg aaggccgctt
     4801 atgccagata cttttgagct aatggttcca gctatgcttc cagagggaat ggctcgagag
     4861 agattttgtg ggtcaacacc tgccaattta caaatttttc ccgaccattc ttttttgact
     4921 acatcaaaaa gcatagttcg agccgccaaa gaatagtcga ttactggttg atcttccgtc
     4981 aatcgataaa taatataatc ctcaacacaa caaaacttcc aggttttttt aaaaacgtct
     5041 ggacaatggt ctttgagcca taatatttta ttaaccgtat acataccatg cagaggcatg
     5101 ccggttattc gaaaaatatt ataagggttt tctctttcta accagtaatc aatataagta
     5161 tgggctcgag tatcgaaatt tgccagagca ttataaagtg ccttaccaga tctatcaatt
     5221 ggaataagcg tttccccatg agaggtaacg gctacgactt ctattggttc ttttgacttt
     5281 ttcgtagcct cgttaagtgc atcaagaacg ctttcccaaa catgttcggg attaatctcc
     5341 aaccaacccg gctgaggtgt tataaaagga tattcacggt atgattgagc aaccatagtt
     5401 ccatcttcac gaaaaaggac aactttacac ccactcgttc ctacatccaa accagccaaa
     5461 accaatgata ccctctccct ccatcaattc gatgtatttt tatatttttt atggcaaaag
     5521 ccttgaatct aaggtaaaat ttaaatttcc cgttttctat cctgaaaacc aacgggcatg
     5581 ataacaagcc cctaccaaag aatataaaat tgtaggggca caatgtattg tgcccattc
//
LOCUS       DAOSOL010000065         5620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14562_length_5620_cov_3.861456, whole genome shotgun sequence.
ACCESSION   DAOSOL010000065 DAOSOL010000000
VERSION     DAOSOL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14562_length_5620_cov_3.861456"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /gene="malQ"
                     /locus_tag="PK171_02520"
     CDS             complement(<1..1705)
                     /gene="malQ"
                     /locus_tag="PK171_02520"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOT05110.1"
                     /translation="MNELRKLAELYGIETNYYDIHGSHIQIEDEVLVNLLGSFGEYPQ
                     NRSGWVENIHRKKEEIAFPIPPVIVTSTREIAFVPQLNWLSGSLFLARENVILEIPFR
                     VENHKWSFPLPLDLEWGYYPLNFEIRTTLNTLYLKSYFIFTPKYCYLPEEKNFWGLNL
                     AVYSLNTRRKQGIGDLQDLRLVSDLLQKYGGNFLGILPIHLLENRMPYGISPYYPMDR
                     LEWNPIYLPLEWIAQILEINLEHNSTEFCSLLNDQSSQNRSLLDYESAWKGKDQILRN
                     FFQIWQKKAIKPSSLSKKSFSDFLTDGGKRIIHSAFFQAYALKQGWDFRSWPQDFQRG
                     DEEAVAQFINKNHEEVNYFLFLQWLMEIYLKTYFQLSNMLAFDLPVGTSPTGIESWLN
                     QDLFVFSQRVGAPPDDFSPAGQNWGFPPVNPWKDREKHYSHFISLIQANMKYCSFLRI
                     DHIMGLYRLFWIPEGCDPKHGTYVRSFFQDLLGIVALESHRRQVIVIGEDLGTVPPQV
                     REAMKAYKILSTRVLYFESDHKGFIPAPECYPRETMVTIGTHDMPPLKAFLEGKDIDL
                     RE"
     gene            1896..3017
                     /locus_tag="PK171_02525"
     CDS             1896..3017
                     /locus_tag="PK171_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05111.1"
                     /translation="MDFAIALLHGTVDLFVEFLSPISPYLIKTYSIQARTIAMLIASI
                     MLMTALSQILFAMVLPRIKKQWLLLYGIVAFVVLPTSLFSLKMNIVGLYLVFFTVFLA
                     NAAYHPFGTALANRNQSSKTVTSFVSGGIIGGALGPIFITWWVGSVGLNKIIFFNLLI
                     FIILQPILLKVKRNFTPITKHPNFSWKSFPILIPIWIMVGLRTFYMSSLHTYSPMYSN
                     LRGYSLIIGGSLLSLGLVSGLLFNILGSRFRIRFNNWLVNVVSFAGMGLFLNIFVQIE
                     SLALFILFYVLSDSFMFFSMSSNVIEAQKMLPGHPAFAASVSMGVAWATGYLLHLGYS
                     SFFGNQVGFVLNSIGIFSLITAGCLLIFSRYFGRAPKGS"
     gene            3014..4201
                     /locus_tag="PK171_02530"
     CDS             3014..4201
                     /locus_tag="PK171_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT05112.1"
                     /translation="MKKRFFLFRYFSTQFLILMLVAFVANFTYISTSILFALFAKKIG
                     LNVTETGIVISALTLGALIGGPIWGKFVDRTGKRKITILISMIGQGVFCLITPFISGF
                     FPLLMVRFGFGWFWVAQTPIVNEIIIKKRNIEAKNRELSWINIIRGVSYSIAMLISGY
                     LSDFDPTWNFYLAGIVSLAIIYPILLLKKVDGLRSQEEGLKIDGKWILRWKNFLFYLP
                     IFLRSTAVNGLSFFIPLFWKEKGLSAAFIGMVLGVSNLIQLMFFPIVGKTCSRKMKNI
                     PSIILFGYALTIIPFIVAPFLYRGISVFVPQSVLSISWVFFYIATTLLLGEIAPYSQR
                     TEALGWMETAFNLGGTIGPITFSFILAGSSNNYPLSFLSFSLLVFSALIIFSSYERKY
                     HHR"
     gene            complement(4222..4743)
                     /locus_tag="PK171_02535"
     CDS             complement(4222..4743)
                     /locus_tag="PK171_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05113.1"
                     /translation="MKSTSQSMIRWVSRTAVLFALTLVIQLLGLPQFITGPLVNAFLL
                     LATMVVGVSSGMVIGLFTPWVAFSRGILPAPMGPMIPFIMLGNVTIVIVFFLITTRRE
                     NNIGFLGLGIVIGSIAKYLVLSQSVAFLVSVPPPVAKMMQFPQLITALVGGVIAIVIK
                     QALGRTNFFKNQS"
     gene            complement(4749..>5620)
                     /locus_tag="PK171_02540"
     CDS             complement(4749..>5620)
                     /locus_tag="PK171_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT05114.1"
                     /translation="GIILDMIKKDIAGFTGLPENLQVVTGGHDHLCAAVAVGVFKQGQ
                     VLISTGTTENLTMALDAIPHIDFSQLKRSFSWGHHAIPERYYGMSGIYSGGYSLEWFL
                     KLINEKYQYLKEMPQDIPKEISLFFPYLLGADYDGARGAFINLEGKMEKADLTKSLII
                     SLGFEYKDLWDIMAQNLNIRVERVTNVGGGTQNDFWMKVKAAVLNEKILVPQDKEGSC
                     KGAAILAGIASGLYKEPEDAYRKTFHLAKIYEPDPDLRESLLPWYRLYLELRDDIRHL
                     NEKIKIIQTGRRK"
BASE COUNT         1614 a         1137 c         1005 g         1864 t
ORIGIN      
        1 tttctcttaa atcgatatct ttcccttcta aaaatgcttt caagggaggc atatcgtggg
       61 ttccaatagt caccatggtt tcccttggat aacattctgg cgctgggata aaacccttat
      121 ggtcactctc aaaatagagg actcgagtag aaagaatttt ataagctttc atggcttctc
      181 ttacctgagg aggaactgtc cccaagtctt cgcctataac aattacttgt cgacgatggc
      241 tttcgagagc aacaattcct aataagtcct ggaaaaaaga tcgaacatag gttccatgtt
      301 ttggatcaca tccttcggga atccaaaata aacgatacaa tcccataata tgatcgatgc
      361 gaagaaaaga acagtatttc atattcgctt gaataagtga tataaaatga gagtaatgtt
      421 tctccctatc cttccatgga ttcaccggtg gaaatcccca gttctgacca gccggagaaa
      481 aatcatcggg gggagctccg actcgctgag aaaatacaaa taggtcctga ttaagccatg
      541 actcaatacc ggttggagaa gttcccactg gcaggtcaaa agccagcata ttcgacaact
      601 gaaaataggt tttcagatag atctccatga gccactgtaa aaacaaaaaa taattaactt
      661 cctcatgatt tttattaata aattgagcga cagcctcttc atcgcctctc tgaaaatcct
      721 gaggccaaga tcgaaaatcc caaccctgct ttaaagcata agcctggaaa aaggccgaat
      781 gaataattcg cttgccccca tcggttaaaa aatcagaaaa acttttctta ctcagagagg
      841 acggtttaat tgcttttttt tgccaaattt gaaaaaaatt gcgaagaatt tgatctttcc
      901 ccttccatgc ggattcgtaa tccagaagtg acctattctg agaagattga tcgtttaaaa
      961 gtgaacaaaa ttctgttgaa ttatgctcaa ggtttatttc taatatttgg gcaatccatt
     1021 ccaggggaag atagattgga ttccactcta accgatccat aggataatac ggactgattc
     1081 cataaggcat tcgattttct aagaggtgga tgggaagaat ccctaaaaaa ttgccgccgt
     1141 atttctgaag gagatcgctc actaatctca gatcttgcaa gtctcctatt ccctgctttc
     1201 ttctggtatt gagtgaataa acggctaggt tcaagcccca aaaatttttt tcttcgggta
     1261 gataacaata ttttggagta aagatgaaat agcttttcag atataaagta ttcaatgtgg
     1321 ttcgaatttc aaaattgagt ggataatatc cccactccaa atccaatgga agtggaaagc
     1381 tccatttatg gttttcaact cggaagggaa tttccaagat aacattttcc cgagcgagaa
     1441 agagtgatcc tgaaagccaa ttcaattgag gaacaaaagc aatctctcga gttgaggtaa
     1501 caataactgg tggaatagga aaggctattt cttctttctt tcgatggatg ttttccaccc
     1561 aacctgaacg attttgggga tattcaccga aagaaccaag taggtttact aaaacttcat
     1621 cttcaatttg aatatgactt ccatgaatat cataataatt agtttcaatt ccatataatt
     1681 cagccaattt tctcaattcg ttcatgaaat cccacctttc actctttcac ctttcatgat
     1741 acactacctc gaaaatggaa tgaataccta ccaaaagttt ttctgttttt tagttcatta
     1801 atggtggcta agtctccaaa ctaactaaat aaatcttatt ataataacaa actgttttta
     1861 aacttagatt tgctggtagg aaaagaggat gttttgtgga tttcgctatt gcactactac
     1921 atggaacagt tgaccttttc gttgaattcc tttcaccgat tagtccctat ttgattaaaa
     1981 cctattcaat tcaagctcga accattgcta tgctcattgc atcaataatg ttgatgacgg
     2041 ccttaagcca aattcttttt gctatggttt taccaaggat aaaaaaacaa tggctcttgc
     2101 tttatggaat tgttgctttt gtagtacttc ctacttcact attttctcta aaaatgaaca
     2161 ttgttggatt gtacctggta ttttttacgg tttttttggc caatgctgcc taccatcctt
     2221 ttggtacagc tttggccaat cgcaatcaaa gctccaaaac ggttacctct tttgtgtcgg
     2281 gaggcattat cggaggggct ttaggtccaa tttttattac ctggtgggtt ggaagcgttg
     2341 ggctcaataa aattatcttt tttaatcttt taatttttat tattcttcaa ccgattcttt
     2401 tgaaggtaaa aaggaatttc acacctatca ccaaacatcc aaatttttct tggaagtcat
     2461 ttccaattct catcccaatt tggatcatgg ttggtttaag aactttctat atgtcttctc
     2521 tccacaccta ttcaccaatg tatagcaacc ttcgggggta tagtcttatt attggtggat
     2581 cccttctctc tcttggcttg gtttcaggcc tgcttttcaa tatccttggt tctcgatttc
     2641 ggattcgatt caacaattgg ctagtcaatg tggtatcttt tgctggaatg ggactctttt
     2701 taaacatttt tgttcagatc gaatctctcg ccttatttat actcttctat gttctttctg
     2761 attcattcat gtttttctcc atgagttcaa atgtcattga agcccaaaaa atgctccctg
     2821 gacatccggc tttcgctgca tcggtatcaa tgggagtagc ttgggcaact ggatatttac
     2881 ttcatttggg ctattcgtct ttctttggaa atcaggttgg ttttgtcctc aatagtatcg
     2941 gaattttctc tcttatcaca gcaggatgtt tactgatatt ctctcgttac tttgggagag
     3001 caccaaaagg atcatgaaaa aaaggttttt tctatttcgg tatttttcca ctcaattttt
     3061 aattttaatg ttggttgcct ttgttgccaa ttttacctac atatcaactt caattctttt
     3121 tgctcttttt gccaaaaaaa ttggtcttaa tgttaccgaa actggaattg tcatttcagc
     3181 tttaacctta ggtgccctaa ttggtggacc aatctggggt aagtttgttg atcgaaccgg
     3241 aaaaaggaaa attaccattc tcatcagtat gattggtcaa ggtgtttttt gtcttatcac
     3301 tccttttatt tctggatttt ttcctttatt gatggtgagg tttggttttg gttggttttg
     3361 ggtggctcaa acaccaattg tgaatgaaat cataatcaaa aagcgaaata tcgaagccaa
     3421 aaatcgtgaa ctgagttgga ttaatatcat acgtggagtc agctattcca ttgctatgct
     3481 catttctggt tatctcagcg atttcgatcc tacctggaat ttttacctag caggtatcgt
     3541 ttctttagct attatttatc caattcttct tttaaagaaa gttgatggat taaggagcca
     3601 agaagaaggt ttaaaaatcg atgggaagtg gatattaaga tggaaaaact ttctttttta
     3661 tctcccaatt ttccttcgat ctacagctgt aaatgggctt tcttttttta ttccactgtt
     3721 ttggaaggaa aaaggtcttt cggcagcttt tataggtatg gttttagggg tatctaactt
     3781 aattcaactg atgttttttc cgatagtggg gaaaacctgt tctcggaaaa tgaaaaatat
     3841 cccatcaata attctgtttg gatatgcatt aactattatt ccttttatcg ttgctccctt
     3901 tctctatcgc gggatatcgg tcttcgtccc tcaatcggtt ctaagtatca gttgggtatt
     3961 tttctatatt gccaccactc tcctgctggg ggaaatagct ccctattccc agagaaccga
     4021 agccttaggg tggatggaaa ccgctttcaa cttaggagga accatcggac ccattacctt
     4081 ttcttttatt cttgctggaa gcagcaacaa ctacccgctt tcttttttaa gtttttctct
     4141 gctggttttt tcggctttga ttattttttc ttcctatgag aggaaatatc atcatagata
     4201 aagtatcgtt cagattaaaa tttacgactg attcttaaaa aaattggttc gacctaaagc
     4261 ttgtttaata acaatagcaa ttactcctcc tactaaagca gtaatcagtt gaggaaattg
     4321 catcattttt gcaactggag gagggacgct tactaaaaaa gccactgatt gagaaagcac
     4381 taaatactta gcaatagacc caataacaat gcccaatccc aggaaaccaa tattattttc
     4441 tcttcttgtg gtaatcaaga aaaagacaat cactattgtt acattcccta acataatgaa
     4501 agggatcatc ggtcccatgg gagcgggtaa aataccccga gaaaaagcta cccaaggtgt
     4561 aaataaacca ataaccattc cacttgaaac tccaaccacc atagttgcta gcagtaaaaa
     4621 ggcattgacc agtggcccag ttataaattg cggtaaacca agcaattgga taactaacgt
     4681 gagagcaaaa aggactgccg ttcggctcac ccatcgaatc attgattgag aggtactttt
     4741 catatttttt attttctcct cccggtttgg attattttaa ttttctcatt caggtgtcga
     4801 atgtcatccc gtaactctaa atataaccga taccagggaa gaagactctc ccttaagtcg
     4861 ggatccggtt catagatttt tgctaaatga aaggttttcc gataggcatc ttccggttct
     4921 ttatacaatc ccgatgcaat tccagccaaa attgctgccc ctttacagct tccttcttta
     4981 tcctggggaa ctaaaatttt ttcatttaac actgctgcct tgaccttcat ccaaaaatcg
     5041 ttttgagtac ctcctccaac attggtcact cgttcaacac gaatatttaa attttgcgcc
     5101 ataatatccc ataagtcttt atattcaaag cccagactga taatcaagga tttcgtcagg
     5161 tcagcttttt ccatcttccc ttccaaattt ataaaagcgc cgcgtgcacc gtcataatca
     5221 gcccccaata ggtatgggaa aaaaaggcta atctctttcg gaatatcttg aggcatttct
     5281 tttaaatatt ggtacttttc gttaatcagc tttaaaaacc actccaaaga atatcctccg
     5341 ctgtaaattc cactcattcc ataatagcgt tccggtatgg catgatgtcc ccagctaaaa
     5401 gagcgtttaa gctgagaaaa atctatatga gggatggcat ctaatgccat agtgagattt
     5461 tcagtggtac cggttgaaat aagaacctgg ccctgtttaa atactcccac tgcgactgct
     5521 gcacacaagt ggtcatgccc accggtaact acttgaaggt tctccggcaa tccggtaaaa
     5581 ccagcgatat ccttttttat catatctaat ataattcccg
//
LOCUS       DAOSOL010000066         5617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14567_length_5617_cov_3.258360, whole genome shotgun sequence.
ACCESSION   DAOSOL010000066 DAOSOL010000000
VERSION     DAOSOL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14567_length_5617_cov_3.258360"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PK171_02545"
     CDS             complement(<1..485)
                     /locus_tag="PK171_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT05115.1"
                     /translation="MNQFKPINDQDIQYFKTIIGEQFVSGDPEVLKNYSKDETPDETL
                     RLLPEVVIKPTTNKEIQLITRYCFEKNIAITPRGAGSGLSGGSIPAYGGVVLSLERMN
                     RILEIDIENLIVVVEPGVITNDINRQLKSYGLFYAGYPMSLETCQIGGNVAENAGGGK
                     AV"
     gene            complement(482..1468)
                     /locus_tag="PK171_02550"
     CDS             complement(482..1468)
                     /locus_tag="PK171_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT05116.1"
                     /translation="MNHHLLIIGEIRRGKVDPTTFEILGAAQQLRSKGVEKVGVFFGS
                     SEFNIEELQSPLQFGANYVYGIKNPIFDELAFHQSTQFIINIIQKFKPTIILAAATTQ
                     GTTLMPYLAAKLHTGLTADCTGFDIDSKDGALLQIRPAIGGNIMATIKTRTFPQMATV
                     RPNTFKPVQSEFPDSALVIEEDNSTYSSGFLKNEFIEGNHNLDIAKAKILCSGGRGLK
                     NAKNFFLLEELAQYLNGCVGGSRVAVEQGWTSFPHQVGLSGKTVSPEFYIALGISGAI
                     QHLAGMQTSRYIIAINRDPEAQIFHLADFGIVGDVLEILPQVIRILKQRKNK"
     gene            complement(1469..2257)
                     /locus_tag="PK171_02555"
     CDS             complement(1469..2257)
                     /locus_tag="PK171_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT05117.1"
                     /translation="MEMIVLIKQVPEIGEAKIDPQTGTLNRKGIPLTINPIDLNAIEL
                     ALQIKEEHGGKVRVITMGPESAEKALREALALGCDEAFLLTGQEFAASDTYATAYALT
                     QLVTFLKPWEIIITGEKAIDGDTGQVGPAVASFLNIPILTYAIQCQLIKSSLFLIERT
                     IESGVQRITLPPPFLITVTKGMNVPRFPTLRGKICARQQEIQKLNAHSLGISVDSVGL
                     SGSPTRVVKVENFKIERRGKILKDMDPTIMADQLVQFLIDRGAW"
     gene            complement(2577..3785)
                     /locus_tag="PK171_02560"
     CDS             complement(2577..3785)
                     /locus_tag="PK171_02560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT05118.1"
                     /translation="MKRLDDYQEIVGDEVIFRIHNKMTKLYGKHIVHVNSTYQGGGVA
                     EMLSSLVPLMNDIGLDAGWRILHGNPDFFGITKKFHNALQGDPLHFTHIKRDLYVKNN
                     EDFSQFTHLDHDACIIHDPQPLPLIRFYKKRQPWIWRCHIDLTDPNQRLWNFLKSFIL
                     KYDLVIISNEKYLRKDLPVRQIIIPPAIDPLSPKNKPLTEYTIQKTMKKFRVPLNKPI
                     LTQISRFDKWKDPEGVIDIYREVRKAIDCRLVLCGSMASDDPEGYKIYSKILKKHTDL
                     IKKRDLILLTTENSILVNALQRQANVVIQKSTREGFGLTVTEALWKGKPVIASNIGGI
                     PLQIQDGESGFLIDPHDKKGTVEKIISLLEDPEMGVEMGEKGKEHVRNNFLIIRLLEN
                     YLDLLSELIK"
     gene            complement(3782..>5617)
                     /locus_tag="PK171_02565"
     CDS             complement(3782..>5617)
                     /locus_tag="PK171_02565"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HOT05119.1"
                     /translation="KVNKIFLDTVAEILKPDDLVWIHDYQLMLLPNMICEMFPETVIG
                     FFLHIPFPSYEIFRLLPWRNEILMGFLGSNLIGFHTYDYARHFQSSVRQLLGLDSILG
                     QINVNGRIVKIDAFPMGIDYHFYSNAGKNVEIVEESEKLKEKLGNRKIIISVDRLDYT
                     KGIINRLKAIDLFIEKNPEYEKKFTLVLVVVPSRIGTKNYFLLKKQVDEMVGYINGKY
                     GSIEWIPIWYLYRQLPLINLTALYNIADVALVTPLRDGMNLVAKEYLATKTEKKGVLI
                     LSEMAGAARELGEAIIVNPNDIEAIVEAIRRAFSLSDEEQIEMNRIMNRRLSRYTIEK
                     WAKEFLDNLIKARDTQLEYSTKKLTKTIRNRIQNDYVTAHHRLLLLDYDGTLVSFVDA
                     PRKAKPDEKLLTLLEKLNNDERKQVVIISGRKKDTLESWFDLSRLILVAEHGAWIKKV
                     SADWQTIEPLSNQWKEQIRPLIEHYVDWTPGSFLEEKDFSLVWHYRKADPNLADVRTK
                     ELENALLDLIANLNLGVLRGNKIIEVKNVSINKGRIASQLLNDYPTDFILAVGDDYTD
                     EDIFSVLPETAHTIKVGIGSTHAHYSIQSPGDVRQLLLELTEVTQ"
BASE COUNT         1669 a         1141 c          983 g         1824 t
ORIGIN      
        1 actgccttgc cccctccagc attttcagca acatttcctc ctatctgaca ggtttccaga
       61 ctcatcggat agccggcgta aaaaagacca tagcttttta attgacggtt aatgtcgttg
      121 gttatgactc ctggttcgac aaccacgatt agattttcaa tatcgatttc taaaatccgg
      181 ttcattctct ccaaagacaa cacaacacca ccataggctg gaattgaccc acccgaaaga
      241 ccactgcccg ctccccgcgg ggttatcgct atattttttt caaaacaata tcgggttata
      301 agctggattt ccttattggt ggttggtttt ataaccactt cgggtaataa tcgcagagtt
      361 tcatcgggag tctcatcctt tgagtaattc ttaaggactt ctggatctcc agatacgaat
      421 tgctctccaa taattgtttt aaaatattga atatcctgat cattgattgg tttgaattga
      481 ttcatttatt tttcctctgt tttaaaattc gaatgacctg agggaggatt tctaatacat
      541 ctccaacaat accaaagtcg gcgagatgaa agatttgcgc ttctgggtcc cgattgatag
      601 ctatgatata acgagacgtt tgcattccag ctaaatgctg aatcgctcct gatattccaa
      661 gagcaatata aaattcaggt gatacagttt ttccgctcaa acctacttga tgaggaaaag
      721 aggtccatcc ttgctctacc gccactcgac ttccacccac acaaccattt aaatattgtg
      781 ctaattcttc aaggagaaaa aagtttttgg cattttttaa tccccgaccg ccactgcata
      841 ggatttttgc tttggctata tcaagattat gatttccttc gataaattca ttttttaaga
      901 aaccactgga gtaggttgaa ttatcctcct cgatcacaag agctgaatca gggaattcac
      961 tctgaactgg cttaaaagta ttgggacgaa ctgttgccat ttgggggaag gttctggttt
     1021 ttatagttgc cataatgttt ccaccaatag ccggtcgaat ttgcaacaga gccccgtctt
     1081 tagagtcgat atcaaaacca gtacaatcag cggttaagcc agtatggagt ttagctgcca
     1141 gatagggcat gagagtggtt ccttgggtgg tagctgcagc taaaatgatg gtaggtttga
     1201 atttttgtat gatatttatg atgaattgtg ttgactggtg aaaggctaac tcatcaaata
     1261 tgggattttt aatcccgtaa acataatttg ctccaaactg aagtggactt tgcaattctt
     1321 ctatattgaa ttcgcttgat ccaaaaaaaa cgccaacctt ttccactcct ttactacgaa
     1381 gctgttgagc tgctcctaat atctcaaaag tagtcggatc gactttaccc cgacgaattt
     1441 cacctatgat cagtaaatgg tgattcattt accaagctcc tcgatcaatt aaaaactgaa
     1501 ccaattgatc tgccatgata gtcggatcca tatcttttaa aatctttcct cttcgttcaa
     1561 ttttaaagtt ttcgactttt acgactcggg taggcgaacc agataatcct accgaatcca
     1621 ctgaaatacc cagagaatga gcatttaatt tttgtatttc ttgttgccgg gcgcatattt
     1681 tccccctaag ggtaggaaat cgggggacat tcatcccttt ggtaacggta atcaaaaaag
     1741 gaggaggtag cgtgattctt tgaactccgg attcaatggt tctttcgatt aaaaaaagag
     1801 agcttttaat caattgacat tgaatggcat aggtgagaat aggaatattt aaaaacgatg
     1861 ctacagccgg gccaacctga ccagtatcac catcaatagc tttttctccg gtaatgataa
     1921 tttcccaagg ttttaaaaaa gtaaccaatt gggttaaggc ataagcagtt gcataggtat
     1981 cggaggcggc aaattcctga ccggtgagaa gaaaagcctc atcgcatccc agggctaatg
     2041 cttctcgtaa ggctttctcg gccgattctg gtcccatagt tattactcgt accttaccgc
     2101 catgctcctc ttttatctga agagccaatt caatggcatt caaatcgatt ggattaatgg
     2161 tgagtggtat tccttttcga tttaaggttc cggtctgggg atcgatctta gcctctccaa
     2221 tttctggtac ctgcttaatc aatacaatca tttccatgga taaaaacctc ttcgattttc
     2281 aatttcatct aatgactcat tgaacaaatc ccttccaatt tttcaatgtt aactttttgc
     2341 tctaataaaa tcaatgatcc caattgaaaa taatgcccct tttatcattc cattaaaagt
     2401 aggtcaaaaa gaaagaatga acttgaaatg aagaatctca aagctggaca taaaaaagta
     2461 actatctttt tgattttttt aataaacctc tctttgagcc atttccaaag ctccgaaaag
     2521 tttttgatac accaactaat tcaaataaat gattaaagag aatttattta ctttctttat
     2581 ttgatcaatt cggataggag atcgagataa ttttctaata atcgaattat caggaagtta
     2641 tttcggacgt gctctttccc tttttctccc atttctactc ccatttcagg atcttcaaga
     2701 agagaaatga tcttctcaac agtgcctttt ttatcgtgag gatcgatcaa aaagccgctt
     2761 tctccatctt gaatttgtaa ggggatccct ccaatgttgg aagcaatcac cggtttaccc
     2821 ttccaaagtg cttcggtcac cgtcaagcca aaaccttctc gggttgattt ctgaataaca
     2881 acatttgctt gacgctgcaa ggcgttaact agaatactat tttcggtggt aagcagtatt
     2941 aaatctcgct ttttaataag atcggtatgc ttttttaaaa tttttgaata aattttatat
     3001 ccttcgggat catcactggc catactccca cacaaaacca atcgacaatc tattgctttt
     3061 cgaacttctc gataaatatc aatgactcct tcgggatctt tccatttatc aaaacgagaa
     3121 atctgagtaa ggatcggttt attgagagga actctgaatt ttttcatggt tttttgaatc
     3181 gtatattcgg taagtggttt attttttggt gacagaggat caatggctgg tggaatgata
     3241 atttgcctaa ctggaagatc ttttctgaga tatttttcat tcgaaataat taccaggtca
     3301 tattttaata taaaactctt taagaaattc cagagccgtt gattgggatc ggtcagatca
     3361 atatggcacc gccaaatcca tggttgtctt tttttataaa aacgaattaa gggcaatggt
     3421 tggggatcgt gaataatgca agcatcatgg tcaagatggg taaattgcga aaagtcttcg
     3481 ttgtttttaa catataaatc cctcttaata tgagtaaaat ggagagggtc tccttgcagt
     3541 gcattgtgaa atttcttagt gattccaaaa aaatccggat tcccgtgcaa gattctccaa
     3601 ccagcgtcga gaccaatatc attcattaag ggcaccagcg aagagagcat ttcggctacc
     3661 cctccaccct ggtaggtcga gttaacgtga acaatatgtt tcccatataa tttcgtcatt
     3721 ttattatgaa tccgaaatat aacctcatct ccgactattt cttggtagtc gtctaatctt
     3781 ttcattgagt cacctccgtt aattccaata ggagctgtcg aacatctccc ggactttgta
     3841 tactatagtg ggcatgagtt gaaccaatcc ccacttttat tgtatgagca gtttctggta
     3901 acactgagaa gatatcttca tcggtataat catcaccaac ggctaaaata aaatcagttg
     3961 gataatcgtt taataattga ctcgctatcc gacctttgtt gatggataca tttttaacct
     4021 caatgatttt atttcctctc aaaactccca aattaagatt ggcaattaaa tcaagcagag
     4081 cattttccag ttctttggtc cggacatcag ctaagtttgg gtcggctttt cgataatgcc
     4141 ataccaatga aaaatctttt tcttctaaaa acgaaccggg tgtccaatcg acataatgtt
     4201 ctatgagcgg acgtatctgc tctttccatt ggttcgataa aggttcaata gtttgccaat
     4261 cagctgaaac tttcttaatc caggcaccat gttcagcgac caatatcaat cgggataaat
     4321 caaaccagga ttctaaagta tctttctttc taccgctaat tatcactacc tgtttcctct
     4381 cgtcgttatt caacttttcc aacaaagtaa ggagcttttc gtcgggtttg gcttttcgag
     4441 gagcatcaac aaaagaaacc agtgttccat cataatccaa caacaacaga cggtggtgag
     4501 cagtaacata atcattctga atacgatttc gaatggtttt agtcagtttt tttgtcgaat
     4561 attctaattg tgtatctctc gccttgatta aattatccaa aaattctttc gcccattttt
     4621 caatcgtata ccgtgataaa cgacgattca taattcgatt catttcaatc tgctcttcat
     4681 cagaaagaga aaatgccctt ctaattgctt caacaatagc ttcaatatca ttcggattaa
     4741 caataatggc ttctcccaat tctcgagctg ctccagccat ttcgcttaga attaatacac
     4801 cttttttctc agtttttgtt gctaaatatt ccttagcaac taagttcata ccatctcgta
     4861 atggtgttac caatgcaaca tcagcgatat tataaagagc tgtgagattt atgagtggca
     4921 attgacgata gagataccaa ataggtatcc attcaataga accatatttt ccgtttatat
     4981 aacccaccat ttcatcgact tgctttttca aaagaaagta attcttggtt ccgatccgtg
     5041 atggaacaac caccaaaacc agggtgaact tcttttcata ttctggattt ttttctataa
     5101 atagatcaat ggcttttaat cgatttataa tccccttggt ataatccaat cgatcgaccg
     5161 aaatgataat tttccgatta ccaagttttt ctttcaattt ttcactttct tcaacaattt
     5221 ccacattttt acctgcattc gagtaaaaat gataatcaat tcccatggga aaggcatcga
     5281 tttttacaat tcttccattc acattaattt gacctaatat agaatccaat cctaataatt
     5341 gccgaacact cgattgaaaa tgccgggcat agtcataagt atggaagcca attaggtttg
     5401 atccgagaaa acccattaag atttcatttc gccaaggaag taggcgaaat atttcatatg
     5461 aagggaatgg aatatgaaga aaaaatccta taactgtttc aggaaacatt tcacaaatca
     5521 tattaggaag aagcatcaat tgataatcat gaatccaaac caagtcgtcg ggtttaagaa
     5581 tttcagcaac ggtatctaaa aatattttat tgacttt
//
LOCUS       DAOSOL010000067         5608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14594_length_5608_cov_3.328111, whole genome shotgun sequence.
ACCESSION   DAOSOL010000067 DAOSOL010000000
VERSION     DAOSOL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14594_length_5608_cov_3.328111"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /gene="gcvPA"
                     /locus_tag="PK171_02570"
     CDS             complement(<1..786)
                     /gene="gcvPA"
                     /locus_tag="PK171_02570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOT05120.1"
                     /translation="MRYIPTTEQDRKDMLKSIGLQSMEGLFDDVPDRVRLQGELNLPS
                     AQTEYELFKSLKSLAEKNNNLEQYPSFLGAGAYGHLIPSAVQHILSRSEFYTAYTPYQ
                     PEISQGVLQAIYEYQSLICSLTGMDVANASLYDGATALAEAAVLAGEATKRKKIFLPM
                     TIHPEYRMVTQLYLENRGMEVIQIPHDKGQTDLNYLKDNINEEIAGVLIQQPNFFGIL
                     EEVKEVGSLTQKFGALLVVSVNPISLGILEAPGHYGADIAVGEG"
     gene            complement(812..1201)
                     /gene="gcvH"
                     /locus_tag="PK171_02575"
     CDS             complement(812..1201)
                     /gene="gcvH"
                     /locus_tag="PK171_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT05121.1"
                     /translation="MMYPEDRYYTKSHEWIKVEGNHATVGVTYYAQEQLVEVVFVELP
                     EPGMEVKAGDGIAVLESVKAVSDIYAPVSGTILEVNKKLEDQPELINKDPYGEGWIFS
                     LEIIQKEEIDKLIRATEYQSLIENEPK"
     gene            complement(1220..2344)
                     /gene="gcvT"
                     /locus_tag="PK171_02580"
     CDS             complement(1220..2344)
                     /gene="gcvT"
                     /locus_tag="PK171_02580"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT05122.1"
                     /translation="MVLNTENDMGQTKITPLKDIHGKLGAKMVPFAGWEMPLFYKTIT
                     YEHEAVRTKAGIFDLTHMGELRISGKNCELELAQLTTNDPTRLLPGRIQYSLLCQPNG
                     GIIDDILVYRLEDGFFLVVNAANTTKDYEWIKSHLKGTAQVENLTDSKALIAIQGPES
                     ENIVTELEPAVFGLKYYHFLETKVFGSKAIISRTGYTGEDGFELYIPWEQAIKVWDSL
                     EAIARKKRIDLIPIGLGARDTLRLEMRYPLYGNDIDDETTPLEANLEWVVKFDHQFIG
                     KESLLEQKRTGIKKCLVGFIAIDRGIPRQGYPIVYHKERVGMVTSGTFSPSLKKNVGM
                     GYVSPSLAKPGTIVDLLIREKIVRAEIIEGPFVSPKIKKR"
     gene            complement(2389..2739)
                     /locus_tag="PK171_02585"
     CDS             complement(2389..2739)
                     /locus_tag="PK171_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05123.1"
                     /translation="MIGLYTIAKPNHRIFHSDGTLQWIWKTICKEVDVLFSFDPSLDI
                     DKIRQENKDIFLIGNIDPVKIMLEGKSEEIVKISWEKIRAGGKNFALGLGGELVSGTP
                     EENIKVISYLQDEF"
     gene            complement(3036..3932)
                     /locus_tag="PK171_02590"
     CDS             complement(3036..3932)
                     /locus_tag="PK171_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOT05124.1"
                     /translation="MMKALNFLVILFLIGFLIIILGGSQSVQASIETIQVVASISPLA
                     DLMTQVGGERVKVHLLVPNGVSPHTFDPKPSDIKKLEQADLFFIVGLGLEMFAYDMAQ
                     AVGNSLELIEVGKVVETIDDHEENDHEHQFGNPHVWVSLQNLSILAESVAEALIKIDP
                     DNKEIYLKNAQSYIEQCQQLDRWFSEKIKNSKTRHFIAAHSAWDYLARDYNLVQIGVI
                     EEKPGEEPSPKKIKTLIDDLKSSESPVIFAEPQLSQKAAQILAQETGVKVIILDPLGL
                     YPDVPFLKTMEDNLEKIIEAMK"
     gene            complement(4109..5344)
                     /locus_tag="PK171_02595"
     CDS             complement(4109..5344)
                     /locus_tag="PK171_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOT05125.1"
                     /translation="MFIDEFELFLFDADGVIYIGDQLCLGAAEVISWLQKRGKGVRIL
                     TNDSRFSREEIQEKLSRMNIFLDKEDIITASWCAAYYLHKMKASKVLVVGTDALKREI
                     TEMGVTLVDDSPEAIVIGFNEDLKLKEIQQAIRYVERGVAFIATNGDISYPTPQGRQP
                     ATGSIVRLIENVTGRKAFIVGKPSALIFQIAQNGYSPETRTIMIGDSPEIDILGAHQV
                     NIPAVLVSAQLIQYPTLRDYRIPDGQVNNLFQLLEESFQLYPWKKPDFPWPEKIEVAV
                     AAFILNKNKQILLIKRKDNSYWALPTGRMERGETLEEAVIREIKEELNLKIRVKNLVG
                     LYSHPSDQVLSYNVGEIIQFVTACFFCELEEGDLRNNQTEILESGFYSISKFPQPMVY
                     SHQRWIEDGMRNQRSAVFR"
BASE COUNT         1666 a         1178 c          893 g         1871 t
ORIGIN      
        1 accttctcca acggcaatat ccgcaccata atgacctggt gcttccaaga tgcccaaaga
       61 gattggattc acactgacaa ccaatagagc tccaaatttc tgggttaaag aacccacctc
      121 tttcacttct tctaaaatac caaaaaaatt tggttgttgt attaatacac cggcaatttc
      181 ttcgttgata ttatccttta aataatttaa atctgtttgt cccttatcgt gtgggatttg
      241 gatgacttcc ataccacggt tctcaagata gagctgagta accatccgat actcgggatg
      301 aattgtcata ggcaggaata ttttcttccg cttggtagct tcaccagcca aaacagcagc
      361 ttcagccaga gcggtggctc catcatacag cgaagcattc gctacatcca tcccagtcaa
      421 actgcaaatc aggctttgat attcatagat ggcttgcaaa actccctggc tgatctctgg
      481 ttgataggga gtataggcag tataaaattc tgaacggctt agaatgtgct ggactgcact
      541 ggggattaga tggccataag caccagcacc taaaaaagaa ggatactgtt caagattatt
      601 attcttttca gctaaacttt taagagattt gaacagctca tattcggttt gagcggatgg
      661 taagtttaat tctccttgaa ggcggactct gtctggaaca tcatcaaaaa gtccctccat
      721 ggattgtaac cctatacttt ttaacatatc tttccgatct tgttcggttg ttgggatata
      781 tcgcaatctc tgccacctgc ttaaaataag attattttgg ttcattttcg atgagggatt
      841 ggtattcggt tgcccttatc aacttatcaa tctcctcttt ttggatgatc tcgagggaaa
      901 aaatccatcc ttctccatag gggtctttat tgatcaattc tggttgatct tcaagttttt
      961 tattcacttc taatattgtt cctgatactg gtgcatagat atctgatacg gctttcactg
     1021 actctaaaac tgcaattcca tcaccagctt taacttccat tcctggttca gggagttcaa
     1081 caaaaacgac ttcgaccagc tgttcctgag cataatatgt cacgcctacc gtagcatgat
     1141 ttccttccac cttaatccat tcatgacttt ttgtataata gcggtcttca ggatacatca
     1201 tttttcctcc ataagtgtcc tatctttttt taatttttgg agaaacaaaa ggtccttcga
     1261 taatttcagc tctcactatt ttttcacgaa tcaacaaatc aacaatagtc cctggttttg
     1321 ctaaggatgg acttacataa cccattccaa cattcttctt taaactcgga gaaaaggtac
     1381 cactggtcac cataccaact ctttctttat ggtaaacaat tggatacccc tggcgaggaa
     1441 ttccccgatc gatggcaata aaaccaacta gacatttctt tatcccggtt cttttttgtt
     1501 caagtagcga ttctttccca atgaattggt gatcaaattt tactacccat tctaaatttg
     1561 cctccaaagg ggttgtctcg tcatctatat catttccgta cagcggatac ctcatttcca
     1621 atcttagggt atctcttgct cctagtccga ttggaataag atctattcgc ttttttctag
     1681 caattgcttc caaagaatcc cagactttta tcgcctgctc ccatggaata tatagttcaa
     1741 aaccatcctc tccggtatat ccagtacgtg aaataatggc ctttgaacca aaaactttgg
     1801 tctctaaaaa atgataatat tttaatccga aaactgccgg ttctaattct gttacaatat
     1861 tttctgattc tggaccttgg atggctatca aagcttttga atcagttaaa ttttcaactt
     1921 gagcagttcc ctttaggtgg gattttatcc attcataatc tttcgttgta ttggctgcgt
     1981 ttactaccaa gaaaaatcca tcttctaatc tatacaccaa tatatcgtct attattcccc
     2041 cattaggttg gcaaagcagt gaatattgaa ttcgacctgg caataatctg gttgggtcat
     2101 tggttgttaa ttgagctaat tctaactcac aattttttcc agaaatcctt aactccccca
     2161 tatgggttaa gtcaaaaata ccagctttag tgcgaactgc ctcgtgttca taggtaattg
     2221 ttttgtaaaa taaaggcatc tcccatccag caaaaggaac catcttggct cctaattttc
     2281 catgaatatc ttttaatgga gttatttttg tctgtcccat atcattttca gtgtttaaaa
     2341 ccactgtttt cctgcctcct gagttcttta aaataatact aatttaatct aaaattcatc
     2401 ttgtaggtaa gaaataacct tgatgttttc ttcgggtgtt ccagaaacca gctctccccc
     2461 tagcccaaga gcaaaatttt ttcctccagc ccttatcttt tcccaactta ttttaacgat
     2521 ttcttcggat tttccctcaa gcataatttt tactggatct atattgccaa tcaaaaaaat
     2581 atccttattt tcttgacgaa ttttatcaat atccaaacta ggatcaaagg agaaaagcac
     2641 atcaacttct ttacaaatag ttttccaaat ccattgcaat gttccatcgc tgtgaaatat
     2701 acgatgatta ggtttggcaa ttgtatataa tccaatcaaa gacggaagaa cctcaataca
     2761 taggatttct catagacgct atttcaaata gtttaaaaat atttctaatt taatccttac
     2821 ttaaaaaaat aatagagaaa taataatatg atttaatttt ttaatgaaaa cttttctatc
     2881 ttgacaacat gaaactgaaa aagtggacgt attcatctga aatattgaaa gaggttgttc
     2941 aatatctatc ctatcagaaa atgacgaaat tgaaaggttc taattaccaa tgttttctcc
     3001 tatgttaagg aatccaatcg agtggtttat acaacctatt tcattgcttc tataattttt
     3061 tccaaattat cctccatagt cttaaggaaa ggtacatcag gatataaacc aaggggatca
     3121 aggataataa ccttgactcc tgtttcttga gctaaaatct gtgccgcttt ttgacttaat
     3181 tggggttcgg caaaaatcac tgggctctct gaacttttta ggtcatcaat gagagttttg
     3241 attttcttag gagacggttc ttctccgggt ttttcctcaa taacacctat ttggacgagg
     3301 ttataatccc gggctaaata atcccaagca gagtgggctg caataaaatg acgggttttt
     3361 gaatttttta ttttttcact aaaccaccga tccaattgct ggcattgttc tatatatgat
     3421 tgagcatttt ttaaataaat ttctttatta tcgggatcaa tctttattag tgcttcggct
     3481 accgactctg ctaaaatcga aagattttgc aatgataccc aaacatgggg attcccaaac
     3541 tgatgttcat gatcattttc ctcatggtcg tctatggttt caaccacttt ccccacttcg
     3601 atgagttcaa gactatttcc taccgcttga gccatatcat aggcaaacat ttcaagaccc
     3661 aaaccaacaa taaaaaataa atcagcttgc tccagttttt ttatatctga tggttttgga
     3721 tcaaaggtgt gtgggctcac accattcggc accaggaggt gaactttaac cctttcaccc
     3781 cccacctgag tcatcaaatc agctaaagga gaaattgatg caacaacctg aatggtttca
     3841 atggaagctt gaacactttg gcttcctcct aaaattatta tgagaaaacc gattaaaaac
     3901 aggataacca aaaaattcaa tgctttcatc attaaacctc ctttggcctt aggctttctc
     3961 aaaatgtatt ttaatgtttt aatagcgcac ctttacccat gtcttctatc ataagagaaa
     4021 tcctatatat ctttcttatt atggacatta atcaagaatg aggtgcatgt tcctgctcaa
     4081 atatgatgtc atttagcagt ataaatactt agcgaaatac cgccgatctt tggttcctca
     4141 ttccatcttc gatccatcgc tggtgagaat aaaccatcgg ttgaggaaat tttgaaatcg
     4201 aatagaatcc ggattctaaa atttcggttt ggttatttct caaatctcct tcttctaact
     4261 cgcaaaaaaa acaagcagta acaaactgaa tgatttctcc aacgttataa cttaatacct
     4321 gatccgaagg gtgagaatag agacccacca aattctttac tctaatttta aggtttaatt
     4381 cttcctttat ttcacgaata accgcttctt caagagtttc acctcgttcc attctaccag
     4441 tcggtagtgc ccagtaagaa ttatcctttc ttttaatcaa taaaatctgt ttattcttat
     4501 taagaatgaa tgctgcaacg gctacttcga tcttctctgg ccagggaaaa tccggcttct
     4561 tccagggata cagctggaac gattcttcaa gcagctgaaa aagattattg acttgtccat
     4621 caggaatacg gtaatctcgc aaggtaggat actgaatcaa ctgagctgaa accaaaaccg
     4681 ctggaatatt cacctggtga gctcctaaaa tatcaatttc cggactatca ccaatcataa
     4741 tcgttcttgt ttcaggggaa taaccatttt gggcaatctg aaaaattaaa gctgacggtt
     4801 tcccaacaat aaaagctttt cttccagtaa cattttctat caatctcact attgaaccgg
     4861 ttgccggttg tcttccctgt ggagttggat atgagatatc accattggtt gcaataaaag
     4921 caactccccg ttcaacatag cgaatagctt gttggatctc ttttaatttt aaatcttcat
     4981 taaatcctat gacaattgct tcgggggaat catcaacaag ggtaactccc atttcagtga
     5041 tttctctttt taaagcatcg gttcccacca ccaatacttt agatgctttc attttatgta
     5101 aatagtaggc tgcgcaccaa gaagcggtaa tgatatcttc cttatctaaa aaaatattca
     5161 ttcttgatag tttttcttgg atctcttccc gagaaaaacg tgaatcgttg gtaagaattc
     5221 ttactccttt cccccgtttt tgtaaccaac taatgacttc ggctgcacct aagcaaagct
     5281 ggtctccaat atagattact ccgtccgcat caaataaaaa aagttcaaac tcatcaataa
     5341 acatacagcc tccagaaaag tggaataaag tttttcctca tttttaatca attccttatt
     5401 gatctaaaaa aggacttttc tgtaattatt tagataataa cccaacaatt tttaggtttt
     5461 tccttattca aaggaataac tcctaagagt ttttaagtgt ttttcaatat tttcctcaac
     5521 cctttgacaa tggtcttctc gataatcaaa aaggaatcga taaagttctt ctcgatatac
     5581 tttttgaatc gaaccatagg aaatatga
//
LOCUS       DAOSOL010000068         5597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_14624_length_5597_cov_3.586611, whole genome shotgun sequence.
ACCESSION   DAOSOL010000068 DAOSOL010000000
VERSION     DAOSOL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14624_length_5597_cov_3.586611"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PK171_02600"
     CDS             <1..1068
                     /locus_tag="PK171_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT05126.1"
                     /translation="ADEGEPGTFKDRLILEGDPHSILEAMAIAGFAVGAHQGYIYVRG
                     EYPESIRHLEIAISQALDYGLLGDNLLNSGFSFRIDIRKGAGAYICGEETALLESMEG
                     GRGEPRIKPPYPPQKGLWGKPTVINNVETFANIPPIFLNGPEWFKNIGTPTCPGTKVF
                     TLTGNVINLGLIEVPMGTTLRELVYQAGGGIKNGRELKLIQTGGPSGGTMTPDLLDVP
                     MAFDTLPRYQSALGSGALTVIDDTHCIVDVVKNFCEFFLHESCGKCTPCRIGNKRIVE
                     ILERISSGKGIEEDLEKLTFLGEHIKRTSFCGLGQAAPNPLLRCLDYFREEFESHVIE
                     KRCPFNVCPMESPKKIKSLKV"
     gene            1091..2857
                     /locus_tag="PK171_02605"
     CDS             1091..2857
                     /locus_tag="PK171_02605"
                     /inference="COORDINATES: protein motif:HMM:NF040763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT05127.1"
                     /translation="MAKVIQNRSEKESPEKLVTIFIDGRECQVPENLSLLEACRMEGY
                     HIPTLCYMDGLTPWGGCRICVVEVEGQPNLVASCVTPIREGMKVLTASKKVREARRAN
                     LELILSNHPLDCQLCDRNQSCDLQSLCYEFGIREIRFQGERKTHPIDNSSPSVKRDLD
                     RCILCGRCIRTCAEVQSVYAIDFADRGFNSYVSPSLGLPLGESVCINCGQCVLACPTG
                     ALSEVSHVEMVWEALDDPEKYVILQTAPAVRVSIGEPFGIPIGSISTGKMVAGFRRLG
                     FDAVFDTNFAADLTILEEGTEFINRVKKGGKLPLLTSCSPGWIKFMEHFYPELMENVS
                     TCKSPQQMFGALAKTYYAQKMGIDPMNVVVVSIMPCTAKKYECQRDEMKASGYQDVDY
                     SLTTREAARMLKEKGIDLKEMPEEDFDPALGISTGAAAVFGATGGVMEAALRTVYEVL
                     TGETLARLDFTDVRGIEGVKEATINVGGLEVNVAVAHGLGNARILMDQIRQGGSKYHF
                     IEIMACPGGCIGGGGQPIPTDLEIRMKRIEAIYEVDRHFPIRKSHDNPAIKKLYEEFL
                     GEPNSEKSHHLLHTHYTCREKM"
     gene            3010..3438
                     /locus_tag="PK171_02610"
     CDS             3010..3438
                     /locus_tag="PK171_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05128.1"
                     /translation="MEKVILSIDPGTEKCGIALLDRKGGVIHQAILPVDELEPSINKI
                     SKAYWPIKVIIGNSTGSKRVVQLMKKMKIDYVIIEEKHSTLEARKLYFKMNPPRGLKK
                     LLPKGFLVPSVAIDDWSAVIIGHRYLSHFKSDGDNHDVTQ"
     gene            3422..3982
                     /gene="amrA"
                     /locus_tag="PK171_02615"
     CDS             3422..3982
                     /gene="amrA"
                     /locus_tag="PK171_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOT05129.1"
                     /translation="MMLPNELGSILTNLARRAIEYFLVHRTYLPVPRDLFRELYEQKA
                     GVFICLKIHGQLRGCIGTFHPQHDHLIAEIIYNAVSAAVEDPRFPPVTLKDLPDIHIT
                     VDILTPPESIDSPDQLDPKRYGVIVQRGWRRGLLLPDIEGVDSIQEQIRIARAKAGIK
                     PNEPVELFRFRVERYSELENYEENHR"
     gene            3979..4974
                     /gene="amrS"
                     /locus_tag="PK171_02620"
     CDS             3979..4974
                     /gene="amrS"
                     /locus_tag="PK171_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOT05130.1"
                     /translation="MIEAQYWTAETDQRVRCFLCPHQCRINPGRRGLCQIRENRAGVL
                     YALTYNRISVVHMDPIEKKPLYHFFPGKEILSVGSVGCNLKCQFCQNWEISQTGFVDN
                     LKQTDSQKIVSIAQSQGSIGIAYTYNEPFIWWEFVRETSEKAKKVQLKNVLVTNGYVN
                     SQPLLELLPYIDAMNIDLKAMDDDFYRSYCGGSLTPVLETIELSYQKKVHIEITNLLV
                     TGLNHTPEHLHRLVDFVASISPDIPLHFSRYFPAHRMETPATDPEVLLEAYKIARGKL
                     HFVYLGNYYGTEGQTSYCSQCGKPLITRTGYRTSLGYLENAHCQVCGTPFPLVDE"
     gene            4971..>5597
                     /locus_tag="PK171_02625"
     CDS             4971..>5597
                     /locus_tag="PK171_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT05131.1"
                     /translation="MKRSQLLLIFLFGLAGVFIIWAAIRPNPLQHFIYEGFDTWIEID
                     LPSRHQSLSQNIAAIIQELDEKWDRFDQRSEVWKINQNQEPIEIGNSTCDLILRAQEL
                     QNLTEGFFNIFLGSLMDEWSFNNNPRLPSPDRIQVLTEQVASSTIFINKETKSAQRIG
                     QGKIDLGGIAKGYLVDIIIIELEKKQVSPALVNAGGTVFALGKKFRVGI"
BASE COUNT         1643 a         1061 c         1256 g         1637 t
ORIGIN      
        1 gccgatgaag gtgaaccggg gacctttaag gatcgtttga ttcttgaggg agatccacat
       61 tctatcttag aagcgatggc tatcgccggg tttgcagtag gggctcacca agggtatatt
      121 tatgtccgcg gcgaataccc ggaaagcatc cgtcatttgg aaatagccat ttcccaggcc
      181 ttggattatg gcctcttggg tgacaactta ctcaattcgg gattttcttt ccgaattgac
      241 attcgaaagg gagcaggagc ctatatatgt ggtgaagaaa ctgcgctttt ggaatcgatg
      301 gaaggtggtc gaggagaacc tcgaatcaaa ccaccctatc ctcctcaaaa gggtttatgg
      361 ggaaagccaa cggttatcaa taatgttgag acttttgcca atattccccc aatttttctt
      421 aatggaccag aatggtttaa aaatattggg acaccaacct gtccaggaac caaggtgttt
      481 actttaaccg ggaatgtgat taatctgggg cttattgagg ttcccatggg aaccaccctt
      541 cgagaattag tctatcaagc aggtggggga atcaaaaacg gccgtgagct taaattaatt
      601 caaaccggag gtccttctgg tgggacgatg actcccgact tgctcgatgt tcctatggct
      661 tttgatactc ttccccgtta tcaatcggcg cttggctctg gtgcattgac tgttattgat
      721 gatacccatt gtatagtcga tgtcgttaaa aatttctgtg agttttttct tcatgaatcc
      781 tgtgggaaat gcaccccctg ccgaattggt aataaaagaa tcgttgagat tttagaacgg
      841 atttcatcag ggaagggtat tgaagaagat ttggaaaaac taacattttt aggtgagcac
      901 atcaaacgaa catccttctg tggtttgggc caagcggcac caaacccact ccttcggtgt
      961 ttggattatt tccgtgagga atttgagtct catgtcattg aaaaacggtg tccgtttaat
     1021 gtttgcccaa tggaaagtcc aaaaaaaata aaatcattga aggtctgatt aagagaaagg
     1081 agagaagaga atggcaaaag tcatacaaaa tagaagtgaa aaagaatctc ccgaaaagct
     1141 ggtaacgata ttcattgatg gaagagaatg tcaggtcccg gaaaatttat ctcttttaga
     1201 agcctgccga atggaagggt atcatattcc aaccctctgt tacatggatg gtcttacccc
     1261 ctggggtgga tgccgaatct gtgtggttga ggtagaaggc caacctaatc tggttgcttc
     1321 ttgtgtcaca ccaattcggg aaggaatgaa agttcttaca gcttcgaaga aagttcgaga
     1381 agcccgcaga gccaatttag agctcatttt gtcgaaccat cctttagatt gccagctttg
     1441 tgatcgaaac caatcctgtg atctacagag cctctgttat gaatttggta tccgagagat
     1501 tcgttttcag ggagaaagga aaactcatcc tattgataac agtagtcctt cggtaaagag
     1561 agaccttgat cgatgtatac tttgtgggcg atgcattcgg acctgtgctg aggttcaaag
     1621 cgtttatgct attgattttg ccgatcgtgg gttcaattcc tatgtcagtc cttcactagg
     1681 acttcctctg ggtgaaagtg tctgtatcaa ctgcggtcaa tgtgttttag cctgtccaac
     1741 cggtgctctt tcagaagtga gtcatgttga aatggtatgg gaagccttag atgatccaga
     1801 aaaatatgtt attcttcaga ctgccccagc ggttcgagtg agtattggtg aaccatttgg
     1861 gatacccata gggagtatct ccaccgggaa aatggtagcc ggctttcgtc gcttaggttt
     1921 tgatgcagtt tttgatacca attttgctgc agatttgacc attcttgaag aaggaactga
     1981 atttattaac cgggttaaaa aaggtgggaa attgcctctt ctaacttctt gcagtccggg
     2041 ctggataaaa tttatggaac atttttatcc tgaactcatg gagaatgtgt caacttgcaa
     2101 atcacctcag caaatgtttg gagctctggc gaagacctat tatgcccaaa aaatgggtat
     2161 cgatcctatg aacgttgtgg tagtctcgat aatgccctgt acggcgaaga aatatgagtg
     2221 tcaacgtgat gaaatgaaag ctagtgggta tcaggatgtt gattattctc ttactacccg
     2281 agaagcagcc cgaatgctca aagaaaaggg cattgatctc aaagaaatgc ctgaggaaga
     2341 ctttgatcca gccctgggta tttcaactgg tgcagccgca gtctttggag ctaccggggg
     2401 ggttatggaa gctgctcttc gaaccgtgta cgaagtttta accggagaaa ctttagcacg
     2461 acttgatttc accgatgtac gaggaattga aggagttaag gaagcaacca tcaacgttgg
     2521 tggattggaa gtgaatgttg cggttgccca tggattaggg aatgccagaa tcttgatgga
     2581 ccagatccga caaggaggct ccaagtatca ttttattgag ataatggctt gtcctggtgg
     2641 ctgtattggt ggaggcggtc aacctatccc aactgatctg gagattcgta tgaagagaat
     2701 agaagctatt tatgaggtcg accggcactt tcccattcgg aaatctcatg ataatccagc
     2761 tatcaagaaa ctatatgaag aatttcttgg cgagccgaat agtgagaagt cgcatcatct
     2821 cttacatacc cattacactt gtcgggaaaa aatgtaatga gttggatttt tccaacgatt
     2881 aaaaattctt aattaattca ttccgttccc cttaactgtg taaaagggga acggaatgtg
     2941 tactgcttta ccttaatcct tcccaatctc ccttgtatcg aaattaaatc tcgagtacaa
     3001 tgattttcta tggaaaaggt tattttatca attgatccag gaaccgaaaa atgcggaatt
     3061 gcgcttttag atcgaaaagg aggggtgatt catcaggcaa tccttccggt tgatgagtta
     3121 gagccctcaa taaataaaat atccaaagca tattggccta taaaagttat catcgggaac
     3181 agtactggca gcaagagagt agtacaatta atgaaaaaaa tgaaaataga ttatgtaatt
     3241 attgaagaaa aacacagtac tttagaagca cgaaaacttt attttaaaat gaatccacct
     3301 cgggggttaa aaaagctttt gccaaaaggt tttttggttc cttcagtagc cattgatgat
     3361 tggtcggcag ttattatcgg ccatcgctat ctttctcatt ttaaatcgga cggtgacaac
     3421 catgatgtta cccaatgaac ttggttctat tttaaccaat ttagcaagaa gagctattga
     3481 atatttttta gtccaccgaa cgtatcttcc ggtacccagg gatctcttcc gggaattata
     3541 tgaacaaaaa gcaggagttt ttatttgtct caaaattcat gggcaacttc gagggtgtat
     3601 cggaaccttt catccccagc atgatcattt aatcgctgaa attatttata atgccgttag
     3661 tgcggctgtt gaggatcccc gctttccccc ggttactctt aaagacctcc ccgacattca
     3721 tattaccgtt gatattctga ctcctcctga atcaatcgac tctccggatc agcttgatcc
     3781 aaagagatat ggagttatcg ttcaaagggg ctggcgaaga ggtcttttac tccctgacat
     3841 tgaaggagtc gattctattc aggaacaaat ccgtattgct cgcgccaaag ctgggattaa
     3901 accaaatgag ccagttgagc tttttcggtt ccgagttgaa cgatacagtg aattagaaaa
     3961 ttatgaggaa aatcaccgat gattgaagct caatattgga cagccgagac ggaccagaga
     4021 gtaagatgtt ttctttgtcc tcatcaatgt cggattaacc caggaaggcg tggactttgt
     4081 caaatacgag aaaatagagc cggagttttg tacgctttaa cctataaccg gatttcagtt
     4141 gtccatatgg atccaattga aaagaaacct ttgtatcatt tttttcctgg gaaggaaatt
     4201 ctttcagttg gaagtgtggg ttgtaattta aaatgtcaat tttgtcaaaa ttgggagatc
     4261 tcgcaaactg gttttgtaga caatctcaaa cagaccgatt cccaaaaaat agtttcaatc
     4321 gctcaatctc aaggatcgat aggaattgcc tatacttaca atgaaccttt tatctggtgg
     4381 gaatttgtaa gagaaaccag tgaaaaagca aaaaaggttc aattgaagaa tgtcttggtg
     4441 accaatggat acgtcaattc gcaaccactc ctggaactcc ttccttatat cgatgcgatg
     4501 aacattgatt taaaggcgat ggacgatgat ttttatcgaa gttactgtgg aggttcatta
     4561 acgccggttt tagaaacaat tgaactgagt tatcaaaaaa aagtacatat tgaaatcacc
     4621 aatcttttag taaccggtct taaccacacc ccagaacatc ttcaccgttt ggttgatttt
     4681 gttgcctcaa tcagtcctga tattcctctt catttctctc gatattttcc agcccatcga
     4741 atggagaccc cggcaactga tcctgaagta ctacttgaag cctataaaat tgctcgagga
     4801 aaattacact ttgtttattt agggaattat tacggaactg aaggtcaaac ctcttattgt
     4861 tcacaatgtg gaaaaccctt aatcacccgt acaggttacc ggacttctct tggttattta
     4921 gagaatgctc attgtcaggt ttgtggaact ccctttcctt tggtagatga atgaaacgtt
     4981 ctcaattatt attgatattc ctttttggat tggcaggagt ttttataata tgggcagcaa
     5041 tcaggcccaa tccattacaa cattttattt atgaaggctt tgatacttgg attgaaatcg
     5101 accttccatc ccgacatcag agcttatcac aaaatattgc agctattatc caagagttgg
     5161 atgaaaaatg ggatcgattt gatcaaagga gcgaagtttg gaaaataaac caaaaccaag
     5221 agccaataga gatcggtaac tcaacctgcg acttgatatt acgtgctcaa gagctgcaaa
     5281 atttaactga aggttttttt aatatttttc ttggatcgtt gatggatgag tggagtttta
     5341 acaataatcc tcgacttcct tctcccgatc ggattcaagt tctaactgag caagttgcta
     5401 gttctactat tttcatcaat aaagaaacaa aaagtgctca acgaattggg caaggaaaga
     5461 tagatttagg agggatagcc aagggatacc tggtggatat aattataata gaattggaaa
     5521 aaaagcaagt atccccagcg ctggtaaatg cgggaggtac agtatttgct ttgggaaaaa
     5581 aattccgagt aggtatt
//
LOCUS       DAOSOL010000069         5454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_15082_length_5454_cov_3.810520, whole genome shotgun sequence.
ACCESSION   DAOSOL010000069 DAOSOL010000000
VERSION     DAOSOL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15082_length_5454_cov_3.810520"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>708
                     /locus_tag="PK171_02630"
     CDS             <1..>708
                     /locus_tag="PK171_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOT05132.1"
                     /translation="VIETPPEGRKPVHTVVAPRNQKMIKEAIQHELERKGQVFYVCPR
                     IKSLKKIYDELVLLFPNYQIGIAHGRLSGPDLEEVMEKFYNAEIDILLCTTIIEIGLD
                     VPNANTLIVDPATLFGLSQLYQLRGRIGRFDREAYAYLFYPKRLTHEAQERLDALLEF
                     EGLGTGYKLALRDMEIRGAGNILGQAQHGFIQEIGFSLYTRMLQEEISRLKGENTETN
                     LSTSIILQENAFFPNWYM"
     assembly_gap    709..718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <719..1090
                     /locus_tag="PK171_02635"
     CDS             <719..1090
                     /locus_tag="PK171_02635"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05133.1"
                     /translation="KQENERFSYYQRLLQAQKLNDIEDLRAEIEDRYGRLPREVETLL
                     KITTLRYYGKIAQVDTIEEKNGEIYIGAPIEVLVSLNEKFRNHGVKGLLVNYHGKQTL
                     RIPFQKLNSLLRLFDPMVGKG"
     gene            1145..1957
                     /gene="mazG"
                     /locus_tag="PK171_02640"
     CDS             1145..1957
                     /gene="mazG"
                     /locus_tag="PK171_02640"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOT05134.1"
                     /translation="MDKMSDSEAKKYFGELIELVAILRSENGCPWDREQTRESLKPLM
                     LEEMYEAFDAIDRKNEADLREELGDMLLHIVFQAQIGIEQGSFNIVDVLKQLIFKMKK
                     RHPHVFGDIQVQNVDEVLLNWETIKEGEKERESMLSSIPPSLPALLRAQVIQSRVARV
                     GFDWPNANEVWRKVIEEQNEFEEAWKSHDQESIEEEWGDLVFALVNLSRHLQLQPEDA
                     LQKASQRFAQRFHYMENEIKKQGKKIENLSLEEMDELWNKAKQEIKLIQNNQ"
     gene            1909..2358
                     /locus_tag="PK171_02645"
     CDS             1909..2358
                     /locus_tag="PK171_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05135.1"
                     /translation="MEQSETRNKTYPKQPIDFGDLFNRIKVLLPLVKGWNPDIYLAVN
                     SAGSIVCGILNNFFPREIRTLSIRYQNDHLIVNWDNCGELRDKRVLVVADHFPNEQVL
                     FQLENFLKTKQVLSLVKLVVLGKGVEYSCFPELPEESILPWETDETG"
     gene            2345..2602
                     /locus_tag="PK171_02650"
     CDS             2345..2602
                     /locus_tag="PK171_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOT05136.1"
                     /translation="MRLDKYLKVSKLIKRRTQAQVACKNNRVYLNNHLAKSSSMVKVG
                     DEIKIVSPVMMLRVKVLQIPVNNQYSDDLFSVIEQKKLETE"
     gene            2718..4004
                     /gene="eno"
                     /locus_tag="PK171_02655"
     CDS             2718..4004
                     /gene="eno"
                     /locus_tag="PK171_02655"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT05137.1"
                     /translation="MSTIFDVHAREILDSRGNPTVEAEVTLASGAFGRAAVPSGASTG
                     TFEAVELRDGDKNRYLGKGVTKAVENVNEIIAPEIIGLDALDQAKIDKVLIDLDGTHN
                     KGKLGANAILGVSLAIAKAAAEYAGLPLYRYIGGANACELPVPLMNILNGGKHADSGV
                     DIQEFMIAPCGLKAFKDALRAGTEVFHHLAKVLKRRGYSVGVGDEGGFAPNLHSSDEA
                     LEVIIEAIQLAGYEPGKDVFLALDSAASELYKDGIYVFEREGARRNIDEMIEFYQELV
                     EKYPIISIEDGLAEDDWEGWKKMTFAMGDKIQLVGDDLFVTNKERLIKGIKEKCCNSI
                     LIKLNQIGTLSETLETIEVAKKAGYTAVVSHRSGETEDTTISDLVVACNTGQIKTGSL
                     CRSERIAKYNQLLRIEEELEEAAVFNGRDVFKVKGY"
     gene            4102..4443
                     /locus_tag="PK171_02660"
     CDS             4102..4443
                     /locus_tag="PK171_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT05138.1"
                     /translation="MVSSYYYDGKTEPDVSHKIAFNWRKILFSIIFALILLSWCFGFT
                     GKLIDYYNLLTEEAYLTHEEKRLYESIEHLNSEKQNLNQDWYIEKLAREKLNLAKPGE
                     VLIKVVPSENP"
     gene            4838..5203
                     /locus_tag="PK171_02665"
     CDS             4838..5203
                     /locus_tag="PK171_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOT05139.1"
                     /translation="MIEVNDIVEGSVVGITKFGAFIELEDGKKGLIHISEISNQFVKD
                     VNDFLKVNDKVKAKVIHISDDGKIDLSIKRLNEDEEYLEKLEKSKAMFEQKLNKFLKQ
                     SQDKLTDLKKHQENKRKFH"
BASE COUNT         1767 a          851 c         1204 g         1622 t
ORIGIN      
        1 gttatagaaa ctccacctga aggtcgtaaa ccggttcata ccgtggttgc gcctcggaat
       61 caaaaaatga tcaaagaagc gattcagcat gaactcgaac gaaaaggcca ggttttttat
      121 gtgtgtccaa gaattaaaag cttaaaaaaa atttacgatg agttggtatt gttatttccc
      181 aattaccaaa taggaattgc ccatggacga ctatccggac ctgatttaga agaagtaatg
      241 gagaagtttt ataatgcaga gattgatatt ttactttgca caacaatcat tgagatcggg
      301 ttagatgttc ctaatgcgaa tacgctgatc gttgatccag cgactctttt tggtttgtct
      361 cagctttatc agctacgagg tcggattggg cgatttgatc gagaagccta cgcctattta
      421 ttctatccaa aaagattaac ccatgaagct caggagcgct tggatgctct tttggaattc
      481 gaagggttgg ggaccggcta taaattagcc cttcgtgata tggaaattcg aggagcaggt
      541 aatattttgg gtcaagctca gcatggattt attcaagaga ttggtttttc cctttatact
      601 cgaatgttac aggaagaaat atcccggctt aagggagaaa atactgaaac gaatttatct
      661 acatctatta ttttgcaaga aaatgccttt ttccctaatt ggtatatgnn nnnnnnnnaa
      721 gcaagaaaat gagcgtttta gttattatca acgtttactg caggctcaga aactgaatga
      781 tattgaagat ctgagggcag agattgaaga tcggtatgga cgcttaccaa gggaagttga
      841 aactctttta aaaattacaa ccctgcgtta ttatggtaaa atagcacaag ttgatacaat
      901 tgaagaaaaa aacggggaga tttatatcgg tgctccaatt gaggtactgg tcagcctcaa
      961 tgaaaaattc cggaaccatg gtgtaaaagg tttgttggta aattaccatg gtaagcaaac
     1021 tttaaggata ccatttcaaa aattaaattc attgttacgt ctgtttgatc caatggtagg
     1081 gaaaggataa aaaaaatttc atatagaact acagtttatt gaggatagtg gagatattcc
     1141 ttcaatggat aaaatgagtg attcagaagc caaaaagtat tttggagagt taattgaatt
     1201 ggtagcaatc cttcgaagcg aaaatggttg cccttgggat cgagaacaaa ctcgagaaag
     1261 tttaaagcca cttatgcttg aagagatgta cgaagccttt gatgcaattg atcgaaagaa
     1321 cgaagctgat ttaagagaag agttgggaga tatgttgctt catattgttt ttcaagcaca
     1381 aattggaata gaacagggtt cttttaatat tgttgacgtt ttaaaacagc tcatttttaa
     1441 aatgaagaag agacatcctc atgtatttgg agatattcag gttcaaaacg tggatgaagt
     1501 tttgttaaat tgggaaacca ttaaagaagg cgagaaggaa agagagagca tgctttcatc
     1561 catacctcca agtttaccag ccttactccg ggctcaagtc attcaaagcc gggttgctcg
     1621 ggtagggttc gattggccaa atgccaatga ggtttggaga aaggtaattg aggaacagaa
     1681 tgaatttgaa gaagcctgga aaagtcatga tcaagaatca atcgaagagg aatgggggga
     1741 tttggtattt gcattagtta atctctcccg gcatcttcaa cttcaaccgg aggatgctct
     1801 tcaaaaagct tcccaacgct ttgcccaacg ctttcattat atggagaacg aaataaaaaa
     1861 acaagggaaa aaaattgaaa acctctcttt agaggagatg gatgagttat ggaacaaagc
     1921 gaaacaagaa ataaaactta tccaaaacaa ccaatagatt ttggagatct atttaatcga
     1981 atcaaagtat tattgccatt ggtaaaaggg tggaatcctg atatttattt ggcggtaaat
     2041 tcagccggtt cgattgtgtg cggcatattg aataattttt ttccaagaga aatcaggacc
     2101 ctttccatcc gatatcagaa tgaccattta atagtcaatt gggacaattg tggtgagtta
     2161 agggataaaa gagtattggt ggttgccgat cattttccca atgaacaagt acttttccaa
     2221 ctggagaatt ttttaaagac caaacaagta ttatcattgg ttaagctagt ggttttgggt
     2281 aaaggcgtgg aatactcgtg ttttccagaa ctaccagaag aatcgattct cccttgggaa
     2341 actgatgaga ctggataaat atttaaaagt ttccaagttg attaaaagac gaactcaagc
     2401 tcaggttgct tgcaaaaata accgggttta tttaaataat cacttagcca agtcatcatc
     2461 aatggttaaa gttggagatg aaattaagat tgtttctccg gttatgatgc ttcgggtaaa
     2521 ggttttacaa ataccggtta acaatcaata ttctgatgac cttttttcag taattgaaca
     2581 aaaaaaactg gaaacggagt aagacaacta taggtgtggg aggcattcct tcaaccatac
     2641 tgtagttttt agggaaacta ttgcagtggt taagttcatt gatcttaagt tgacttttta
     2701 aaggagatga taaactcatg agtacaattt ttgatgttca tgctcgagaa attcttgatt
     2761 cccgcggaaa tcctacagta gaggcagaag tgactttagc atcgggtgct tttggtcggg
     2821 ctgctgttcc ttcgggagca tcaactggga cttttgaagc agttgaactc cgtgatggtg
     2881 ataaaaaccg atatcttggc aaaggagtaa ccaaagcagt agaaaatgtg aacgaaatta
     2941 ttgctccgga aattattggt ctcgatgctc ttgatcaagc caagattgat aaagtcttaa
     3001 tagatctgga tggtactcac aataaaggaa aattaggagc caatgcgatc cttggtgttt
     3061 ccttagcgat tgcgaaagct gcagctgaat atgctggtct tccactttat cggtacattg
     3121 gcggtgctaa tgcttgcgag ttaccagttc ctttgatgaa tatcttaaat ggtgggaagc
     3181 atgccgacag tggcgttgat atccaggagt ttatgattgc tccttgtggg ttaaaagcat
     3241 ttaaggatgc tttgcgagct ggaactgaag tatttcatca cttagcaaaa gttttaaaaa
     3301 gaagagggta ttccgtcggc gttggagatg aaggaggttt tgctccaaac cttcattctt
     3361 ccgacgaggc tttggaagtt atcatcgaag ctatccaatt agcggggtat gaaccaggca
     3421 aagatgtctt tttggcttta gattcggcag catctgaatt atacaaagac ggtatttatg
     3481 tttttgaaag agaaggtgct cgtcggaata tcgatgagat gatagaattt tatcaagagt
     3541 tggtggaaaa atacccaatc atatctattg aagatggatt agccgaagat gattgggaag
     3601 gttggaaaaa aatgaccttt gcaatgggtg ataagatcca gttagttggt gatgatcttt
     3661 ttgtaaccaa taaagaacgc ttgataaaag gcattaaaga aaaatgctgt aactccatac
     3721 tcatcaagct caatcaaatt ggtaccctca gcgaaacctt agaaactatc gaagtggcaa
     3781 aaaaagctgg ttatactgca gtcgtatccc atcgatctgg cgaaaccgag gacacgacca
     3841 tatcggattt agttgtggct tgtaacaccg gtcaaattaa aactggctca ttgtgtcgtt
     3901 cggaaagaat tgccaagtat aaccaattgc ttagaatcga agaagagtta gaagaagctg
     3961 cggtgtttaa tgggcgggat gtttttaaag ttaaagggta ttaacttata aaattgacct
     4021 tccctaagga ttttgcctca gaataattga ggtaaaatcc ttagggtaaa gaactcatta
     4081 aggttttggt ggactaagaa gatggtatct tcctattatt atgatggaaa aaccgaacct
     4141 gatgtttccc ataaaattgc atttaattgg aggaagatac ttttttcaat tatttttgct
     4201 ttgattcttt tgtcttggtg ttttggtttt actgggaagt tgattgatta ttataatctg
     4261 cttaccgaag aggcttatct tacccacgaa gaaaagcggc tttatgaaag tattgaacat
     4321 ttgaacagtg aaaagcaaaa tttaaatcaa gattggtata ttgaaaaatt agccagggaa
     4381 aaattaaacc ttgccaagcc gggtgaagta ttgattaagg ttgttccctc agaaaatcct
     4441 tgacaccctc cttcactcat ttgtataata ttttcgcttt tcctttgaaa gattttaaac
     4501 attctttaaa atttactttt ataccgtatg tagagaagtg tgaggtactg ttttttgggc
     4561 agatatccat actatttgtg tggcatcaga tgaggatgaa aataggtatc ctcctcaccc
     4621 tgaccctctc ccacgaaggg gcgagggaaa aaaataaata aaagaatgaa ttagttcctt
     4681 ctcccttgat gggagaaggt gaggatgagg gtgaaagccc aggacaggat gagattctgg
     4741 ttaattattc aatatgagtt acctatgaaa aatcattcct gtgttttcag gatagaaaaa
     4801 aggaaaaatg atacaaagga ggataatgag tcaccccatg attgaggtga atgatattgt
     4861 tgaaggttcg gtagttggca tcactaaatt cggggcgttt attgaattag aggatgggaa
     4921 aaaaggactc atccatattt cggagatatc aaatcaattt gttaaggatg tgaatgattt
     4981 tcttaaggtg aatgataaag taaaagccaa ggttattcac atttctgatg atggtaaaat
     5041 tgacctctcg ataaaacggc tgaatgagga cgaagagtac ttagagaaat tagagaaaag
     5101 taaagctatg tttgaacaaa agttgaacaa atttctcaag caaagtcaag ataagcttac
     5161 tgatcttaaa aaacaccaag aaaataaacg caaattccac tgattttttt aatttataat
     5221 attcattcaa aagagggaag ccaggttttt ttgagcttcc ctctttcgca ttatgctgat
     5281 taaactatag gtagtttttt aggcgatctt tttcttcgat aatttctcca cttttgctaa
     5341 ctactcttaa aaccccttta acaattcgag ttcgaacttg accacaagcc tcagggtttc
     5401 ctaatagatg gggtgcatcc aaaatacctt tttggatggc acgagcaaga gtag
//
LOCUS       DAOSOL010000070         5333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_15519_length_5333_cov_4.604775, whole genome shotgun sequence.
ACCESSION   DAOSOL010000070 DAOSOL010000000
VERSION     DAOSOL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15519_length_5333_cov_4.604775"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1129)
                     /locus_tag="PK171_02670"
     CDS             complement(86..1129)
                     /locus_tag="PK171_02670"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOT05140.1"
                     /translation="MSQQMKAAVMKGIRKVEIENLPVPVPKEDEVLVRIKSVGVCGSD
                     IHYFIEGRIGDYIVEPPFILGHECSGEVVEIGAGVKDLKPGDRVTMEPGIPCGKCENC
                     RVGRYNLCPDVIFWATPPIDGSFCEYVAHPACFTYPIADQVSFEEAALVEPLSVGMYA
                     TRRARVEPGQTALVLGSGTIGLVTIEALLARGLTKVIAVDVVDMRLQKAKELGAFYTI
                     NPQKEDVVKTVKDLTGNKGVHVVFETAGSVATTQMTVEVAKRGGKVVLIGLPSQAEFN
                     FNIIKTIDKELDVLGIFRYANAYPGGVDLLNSGRVNLKSLITHRFPLEKAQEALLFAH
                     EHKAESIKVVVNL"
     gene            complement(1163..2116)
                     /locus_tag="PK171_02675"
     CDS             complement(1163..2116)
                     /locus_tag="PK171_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HOT05141.1"
                     /translation="MIFIENLYQELELWYPLLRLKEEGVETKLVGTGSSDLYSGRNGF
                     PAKPEVTASSITSRDFDGIIIPGGFAPDYLRRYPAIINLVKETFQQGKLIGALCHGPS
                     VLISADIIRGKRITGNRAIRDDIVNAGGEYVDFHTVVDGNILTAQGPNDLPVFMKEVI
                     GFFSQTKPRLKSPFPEGFLYDAQLEIINLSSVVKGTAAVLLFFDSIASPIAQKALVDY
                     NRLSESIHQLGGIFLAVSIDSIYVQKEFSNRLELAYPLYSDVSGDTIRAFGVKGKNDL
                     AEWAVFMIDKNGILRYSENCPGGDIESLPGFKRHLETLFNY"
     gene            complement(2169..2648)
                     /locus_tag="PK171_02680"
     CDS             complement(2169..2648)
                     /locus_tag="PK171_02680"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT05142.1"
                     /translation="MRFSADEVLEMAVELERRGIAFYENLSKKTSDQKSREIFIFLGE
                     EEKKHLEYFQKTKDDLNTQIDFVPDTMDETSNYLGSLIENGVLGKILQGLDLTQGDSS
                     IEKALEVGMEVEKESVKFYESMEIMVPESKKEWLKKVIQEEKKHYAILLGIKKELGK"
     gene            3023..3568
                     /locus_tag="PK171_02685"
     CDS             3023..3568
                     /locus_tag="PK171_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOT05143.1"
                     /translation="MGTIKKPNPVKFFIAVLFENISVLERIIPQLEKEFGLIQWKSPI
                     IDFTHTNYYQEIGEKLKRIFLVFQELFDPEDLANRKILTNQLESQTGDTQITRRINLD
                     PGYFDGGKIVLATTKNFSHRVYIGKGIYAEATIRWEKGDFRPFEYTYPDYASKEYRPS
                     LIQIRSLYREDLSRAQTNEVV"
     gene            complement(3565..>4187)
                     /locus_tag="PK171_02690"
     CDS             complement(3565..>4187)
                     /locus_tag="PK171_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05144.1"
                     /translation="VGPSGAGKTTLINLLLRFYDPDQGSIEVDGIDIRKIKIASLRQQ
                     IGVVLQDSLVLGGTVRENILYGNLNASMDEVIEAAQRAHAHEFIIHLENSYDTDLGDM
                     GKRLSGGQKQRIAIARALLKNPPILVLDEATSSLDPESEDYILDTIHNHIGKDKIVIL
                     IAHSLRMVKDLDRIVLIKDGEIRDIGTHQELLKNSPYYCQIFGRDL"
     assembly_gap    4188..4197
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4198..5290)
                     /locus_tag="PK171_02695"
     CDS             complement(<4198..5290)
                     /locus_tag="PK171_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOT05145.1"
                     /translation="MVKGKSLFRLLNYLKPYGGLVAGALIMGILGAGISLIFPWLVKI
                     IIDQVLIQKNLLLLLFITFGIVFIFFIKGLTSYIQNLWVSKAGFRVITKLRSELYQHI
                     YSLSASFFQENPTGDIISRMTNDVTNIQNLFSHTFMNIFMDLLILIGSIGVCFYINWK
                     LTTLSIVVLPLVGLSIDYLGKKIRGASHLLQSKTAVLTQLIERTVSGIKIIQSYVSGH
                     YEVEKFEKENELNFYLAMKQAKAKALLTPLVELVSSVGLTIMIWFGGREVVFGKLTPG
                     GLIAFLGYIATAAAPLSHISNGIQALQQSLASAERIFEILDTAPRIQDLSESVSIHSV
                     TRGITLKDIYFSYGGEIILKNFSLHIAQGE"
BASE COUNT         1694 a         1091 c          879 g         1659 t
ORIGIN      
        1 ttttggtgac attgaccatt taaccttaat taatatattt ttataaattc ttaaaaaaga
       61 tcatctaaac ttaaaaagca gctgtttata aattgaccac tactttaatt gattcagcct
      121 tatgttcatg ggcaaagagc aatgcttcct gagctttttc caatggaaaa cgatgggtaa
      181 ttaatgattt taaattcact cgtcccgaat taagcaaatc aacacctccg ggataggcat
      241 ttgcataccg gaaaattccg agaacatcca gttctttatc aatggtttta atgatattaa
      301 aattaaattc tgcttgggaa ggaagaccta ttaaaacgac tttccctcct cttttagcaa
      361 cttcaacggt catttgagtt gtagcaacac ttcctgcggt ttcaaacacc acatggactc
      421 ctttattgcc ggtgaggtct tttactgttt tcacaacatc ttctttttgg ggattaatag
      481 tatagaatgc tcccaattct tttgcttttt gtagtctcat atcaacaaca tcaacagcta
      541 ttacttttgt tagccccctg gctaaaagag cttcaatagt caccaagccg atagtgccac
      601 ttcctaaaac taatgcggtt tgtcccggtt caactcttgc tcttcgggta gcgtacatac
      661 cgaccgagag gggttcaacc agagctgctt cttcaaaaga aacctggtca gcaattgggt
      721 aagtaaaaca agcagggtga gcaacatatt cgcaaaatga accatcaatg ggaggtgttg
      781 cccaaaaaat tacatcggga caaagattat aacggccgac acgacaattt tcacatttac
      841 cgcagggaat acctggttcc atagtcactc gatccccagg ctttaaatct ttgactccgg
      901 caccaatttc aacaacttca ccagaacact catgacctaa tataaaggga ggctcaacaa
      961 tatagtcccc aatccgacct tcaataaaat aatggatgtc tgaaccacaa actccaacgc
     1021 ttttaatccg aactaaaact tcatcctctt ttggtacagg aacgggaaga ttttcaattt
     1081 ctacttttct tattcctttc atcactgcag ctttcatttg ttgagacata attagtacct
     1141 ccttttttgt ttattatttt gattaataat taaataaggt ttctaaatgt cttttaaaac
     1201 ccggtaggct ctctatatca ccacccggac aattttcaga ataacgaaga attccattct
     1261 tatcgatcat gaaaactgcc cattccgcta agtcattctt cccttttacc ccaaaggcac
     1321 gaatggtatc accgctaaca tcgctatata gcgggtaagc cagttccaat ctattggaaa
     1381 attctttctg tacataaata ctatcaatac ttacggcaag aaatattcca ccaagctgat
     1441 gaattgattc cgataggcga ttataatcaa ctaaagcctt ctgagcaata ggacttgcaa
     1501 ttgaatcaaa aaaaagaaga actgcagctg ttccttttac aaccgaagaa agatttatta
     1561 tctctaattg agcatcataa agaaaacctt ctggaaaggg actctttaaa cgaggtttgg
     1621 tttgtgagaa aaaaccaata acttccttca taaaaactgg taaatcattg ggtccttgag
     1681 cggtaagaat gttcccatca accacagtat gaaaatcgac atattcaccg ccagcattca
     1741 caatatcatc acgaattgct cgattcccgg taatcctttt tcctcgaata atatctgcgg
     1801 aaatcaaaac acttggccca tgacagagcg ctccaataag ttttccctgt tgaaaagttt
     1861 cttttactaa attgataatt gccggataac gtcgaagata gtcgggggca aagcctcctg
     1921 gaataattat tccatcaaaa tctcgagatg ttattgagga agcggttact tcaggtttag
     1981 ctggaaaacc atttcttccg gaatacaaat cggaagatcc tgtccctacc aacttggtct
     2041 ctaccccctc ttctttgagc cgaagcaaag ggtaccagag ttccaattct tggtataaat
     2101 tctcaataaa aatcattatc ctccgattat tcaactggct taatctcctt catggttcaa
     2161 tattaatatt atttcccaag ttcttttttt attcccagca aaatagcgta atgttttttc
     2221 tcctcttgga taactttctt aagccattct tttttagatt ctgggaccat aatttccata
     2281 ctttcataaa atttcacact ttccttttct acttccatgc caacttcaag tgccttttct
     2341 atgctgctat caccttgagt caggtcaaga ccttggagta tttttccaag aactccattc
     2401 tcaatcaatg acccaaggta attactggtt tcatccatag tatcgggaac aaaatcaatt
     2461 tgcgtgttta agtcatcttt ggttttttga aaatattcta aatgcttttt ttcttcttca
     2521 cctaaaaaaa taaatatttc tcttgatttt tgatcagaag tttttttaga gaggttttca
     2581 taaaaggcta ttcctcttct ttctaactca acagccattt ctaatacctc atccgcagaa
     2641 aatcgcatgg ccataaatat caccctttca taatctttct ttttaattgt aacatgaaaa
     2701 cggttttatt cagtacttag gaaaagcatt gttataaaat cttatatttc tttcaactaa
     2761 agccatatac tatacaaccg cacatggtgg tcatgaaaat attatctttc tcattttgtt
     2821 tatctcctac caagggaaaa taaccaagtg gggagggaga gttaaaattc gatgaaaaga
     2881 tgaaacacat taaactcctt attactctga tccaattgct ccttttccct tggtaggaga
     2941 aggtttggat gatggtgaat caacaataaa tttttatttc gatttatgtt atgattaagc
     3001 aaaaatcgaa aggaaatacc atatgggaac tatcaaaaaa ccaaatccag tcaaattttt
     3061 tattgctgtc ctttttgaaa acatctcagt tcttgaaagg attattcctc agttagaaaa
     3121 agaatttggt ctcatccagt ggaaaagccc cattattgat tttactcaca ccaattacta
     3181 tcaagaaatt ggtgagaagc taaaaagaat ttttctggtc tttcaggaac tttttgatcc
     3241 agaggattta gccaatcgta aaatattaac caatcagctt gagtctcaaa ccggagatac
     3301 ccaaataacc agaagaatca atcttgatcc tggttatttt gatggaggta aaatcgtctt
     3361 agctaccacc aaaaacttct cacatcgagt atatatagga aagggaatct atgctgaagc
     3421 aaccatccga tgggaaaaag gtgattttcg acctttcgaa tacacctatc ctgactatgc
     3481 cagcaaagaa taccgtccct ccctcatcca gatccgcagt ctctataggg aagatttgtc
     3541 tcgagctcaa accaacgagg tagtttaaag atctcttcca aatatttggc aataataagg
     3601 actgttttta agaagttcct gatgagttcc aatatcccgt atctctccgt ccttgatcaa
     3661 aacaattcga tctaaatctt taaccattct taaagaatga gcaatcagaa tgacgatttt
     3721 gtcctttcca atgtggttgt gaatagtgtc taaaatataa tcttcagatt ccggatcaag
     3781 gctggaagtt gcttcatcta aaaccaggat aggtggattt tttaagagag cccgagcaat
     3841 agctattcgt tgtttttgtc cgcccgaaag tctctttccc atatcaccca ggtcagtgtc
     3901 gtaactgttt tctaaatgga taataaactc gtgggcgtgt gctcgttgag ctgcctcaat
     3961 aacttcatcc atagaagcat tcaagtttcc atagagaata ttctctcgaa ctgttccacc
     4021 taaaaccaat gaatcttgaa gaaccacgcc tatttgttga cgaagagatg ctattttaat
     4081 ttttcgaata tcaattccat caacttcaat acttccttga tcggggtcat aaaaccgaag
     4141 aagaagattt atgagggtgg ttttccctgc accacttgga ccaacaannn nnnnnnnttt
     4201 ctccttgggc aatatggagg gaaaaatttt ttaatattat ttctcctcca taagaaaaat
     4261 agatatcttt taatgtgatt ccccgggtaa cactatgaat agataccgat tcgctgaggt
     4321 cttgaattcg gggagcagta tcaagaattt caaaaattct ttcagccgat gctaagcttt
     4381 gttgtaaggc ttgaatacca ttggaaatgt gagaaagagg agcagctgct gtggcaatat
     4441 accccaaaaa agcaatcaaa cctccggggg ttagttttcc aaatacgact tctcgtcctc
     4501 caaaccaaat cattatggtt aaaccgaccg atgataccaa ctcaactaaa ggggttaaaa
     4561 gggcttttgc tttggcttgt ttcatagcca aataaaaatt cagttcattc tctttttcga
     4621 atttttccac ctcataatgg ccactgacgt aggattgaat tattttaata cccgaaaccg
     4681 ttctttcaat gagttgggtt aagactgcag ttttcgactg taaaagatgt gaggcacctc
     4741 gaattttttt acccaaataa tctatactca atcctaccaa gggtaaaacc acaattgaaa
     4801 gagtggtgag tttccagtta atgtaaaaac atactcctat ggaaccaatc agaatgagaa
     4861 ggtccataaa tatattcata aaagtatgag aaaaaaggtt ttggatatta gttacatcat
     4921 tagtcatacg ggaaataata tccccggttg ggttttcttg aaaaaacgat gccgatagag
     4981 aataaatgtg ttgatataat tccgaacgga gtttggtaat cacccgaaaa ccagctttgg
     5041 aaacccaaag attctgaata taagaagtga gtcctttaat aaaaaatata aatacaattc
     5101 caaaagttat gaataataac aataacaggt tcttttgaat aagaacttgg tcaataataa
     5161 tctttaccag ccaggggaaa ataaggctaa taccagcccc taaaattccc ataataagcg
     5221 ctccagctac caatccaccg tagggcttga gataattgag tagcctaaag agacttttcc
     5281 cttttaccat aaaaattctc ctggattaat tgagttaaaa ccgaatcggc tgt
//
LOCUS       DAOSOL010000071         5269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_15772_length_5269_cov_3.867089, whole genome shotgun sequence.
ACCESSION   DAOSOL010000071 DAOSOL010000000
VERSION     DAOSOL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15772_length_5269_cov_3.867089"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PK171_02700"
     CDS             <1..1193
                     /locus_tag="PK171_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOT05146.1"
                     /translation="PDERPDADALVSTWDWNGQKIILPLSGAGVALALAHEYAIYRGM
                     AFSPIEEFIALACVGTVGDVVPLLDENRVIVKYGLKKINQNPGCGLQALLEVVGYSNK
                     DIQSEQIGYIIAPRLNAAGRVEDPRAAFELLISDNYHNALQQAKLLNEFNQKRQKEET
                     KVFSSLFEDKKNQESMEDDIVVVSGKNWSTGVLGIIASRLSERLLRPAIVLSEDETIA
                     VGSARSILGFNITDALDKVSSLLIRHGGHEMAAGLKIPLSNLEIFRQQLNELFGERIR
                     MLKEKNGIVVDAQVTMADVDSQLMLWLKKLEPFGEKNPNPLFVSSDVTVNERWFSGKN
                     RQRLELVLMQNNKMRQHRIDAMIRSENVSDLASSSLIDLIFEIRKGYWNQPYLKIMDW
                     RIKK"
     gene            1208..1723
                     /locus_tag="PK171_02705"
     CDS             1208..1723
                     /locus_tag="PK171_02705"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOT05147.1"
                     /translation="MDLASTIRNIPDFPKAGIQFKDITTLLKNKDAFRQAVDSLFHLF
                     DSKEVDIVVGIEARGFILGSVVAYLLNCGFVPVRKKGKLPATIRSKTYDLEYGNATLE
                     MHVDAIEKNQKVVIIDDLLATGGTVQAVCEMVEEAGGEIVGIAFLIELESLQGRKAIT
                     KYPVYSLVKLD"
     gene            1967..4174
                     /locus_tag="PK171_02710"
     CDS             1967..4174
                     /locus_tag="PK171_02710"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOT05148.1"
                     /translation="MLRNYEVNSIFSKTITLESLQEKIDSYNPNSDRELIKKAYDFSV
                     DAHSGQSRLSGEPYIIHPLSVAHIVADLRMDDSTIAAALLHDVLEDTKNTEAQLKETF
                     GKEITLLVKGVSKLNVGFHFSSKEEEQIENYRRLFVAMAEDIRVIIIKLADRLHNMRT
                     LKYQYRDKKELIARETLEIFAPIAHRLGIGMIQTELENLSLYFLDPESYCSIQQGIEK
                     IRDHREKQIQEATEKLSQRIQQANLVVEIQSRLKNIYSIYLKINRQNVSLEDLHDLIA
                     LRAITNTVEECYAVLGLVHSLWVPVAGRFKDYIALPKSNMYQSLHTTVIGPGGVPMEI
                     QIRTREMHHVAEYGIAAHWRYKEGKSADSADAFNERMAWLRQILEWQQDLRTTHEFVE
                     SLKIDLFDDEVYVFTPKGDLKKLPKGSSPIDFAYLIHTEVGNNCIGARVNKQIVPLDY
                     ELKSGDIVEIISSRSSSGPSLDWLRIAKTPAARNKIRAFLRKKNRDANRERGKLFMER
                     ELEKFTLTSEEQNLVNDKITEFMEANNYKTIDDLHVIIGEGRLTIRQFIGKVVPFEIR
                     KRFQTQRKAETKKTAQTSGDGVIIQGATGLAVKLARCCTPIPGDKIIGYVTLGKGITV
                     HRLDCHNLTRLTQDEERLVEAEWDERYGRFYDAGLVIDALDRPKLLVDITNTVANYGV
                     SIRSVHAHSMMNDARIYLVISVKNKVELDNLFREIKRVSNVRTVRRGGIFGLR"
     gene            4171..4626
                     /gene="dtd"
                     /locus_tag="PK171_02715"
     CDS             4171..4626
                     /gene="dtd"
                     /locus_tag="PK171_02715"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT05149.1"
                     /translation="MRALIQRVNQARVLIDGKIVGEIGKGMVVFLGVGKNDSSSDIDF
                     MVRKIPQLRIFDDAQGKMNISLNEIQGEILLISQFTLYGNCRRGLRPSYDEAATPREA
                     QNFYNQIAQKLRDQDLTIAEGVFGAMMQVELCNDGPVTILLDSKERRQA"
     gene            4623..>5269
                     /locus_tag="PK171_02720"
     CDS             4623..>5269
                     /locus_tag="PK171_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT05150.1"
                     /translation="MSNKVKVAVYTLGDLMTNCYCLCDCNEALLIDPGSPSQELVEFL
                     TSRKLNLRYIINTHGHIDHIRGNDFFKDYFSSVKIIIYETDMAYLNHPNLNLSTDFSQ
                     PYRSPEPDILIRGKKEPFELFDEEVYFYHSPGHTPGSMCLSFPNQKWLFCGDTLFAGS
                     IGRTDLPGGSFQEILESLTMIMQSFDDETRVFPGHGPETTIGNEKKMNPYILEYL"
BASE COUNT         1670 a          917 c         1151 g         1531 t
ORIGIN      
        1 atcctgatga aagaccggat gccgatgccc tggtttctac ctgggattgg aatggtcaaa
       61 aaatcatcct ccctttatca ggtgctggag ttgcattagc cttagctcat gaatacgcca
      121 tctatcgagg gatggctttt tctccaattg aagaatttat tgctttggct tgtgtaggaa
      181 cagttggaga cgtagtccca ttattggatg agaaccgagt aatcgtaaaa tatggtttaa
      241 agaaaattaa tcaaaatcct ggctgcggat tgcaggcgct tttagaagtg gttggttata
      301 gcaataaaga tattcaatca gaacagattg gttatattat tgctcctcga ttaaatgctg
      361 caggaagagt tgaagacccc agagcagcct ttgaactttt gattagtgat aattatcata
      421 acgctcttca gcaagccaag cttcttaatg aatttaatca aaagcggcaa aaagaagaaa
      481 ccaaagtgtt ttccagttta tttgaagata aaaagaatca agagtccatg gaagatgata
      541 ttgtagtggt ttctggaaaa aattggagca caggtgtttt aggaatcatt gcttcccgat
      601 taagcgaacg tctactgcga ccagctattg tgttgagtga agatgaaacc attgcagtag
      661 gctcagcacg aagcatttta ggatttaata ttactgatgc tttagataaa gtctcatcgt
      721 tgctcatccg gcatggtggt catgaaatgg ctgctgggct taagattcct ttaagcaatc
      781 tcgagatatt ccgacagcaa ctcaatgaat tattcggtga gcgaattaga atgttaaaag
      841 aaaaaaatgg tattgttgtc gatgctcagg taaccatggc cgatgtggat tcacagttaa
      901 tgctttggtt aaaaaagctg gaacccttcg gagaaaaaaa tcctaatccg ttgtttgttt
      961 cttcggatgt taccgtcaat gaaagatggt tttctgggaa aaaccgacag cgtttggaat
     1021 tagttctcat gcaaaataat aaaatgagac aacatcgaat tgatgctatg attcggagtg
     1081 agaatgtatc cgatttagct tcaagttcat tgatcgattt aatatttgaa atacgtaaag
     1141 gatattggaa tcaaccctat ttaaaaatta tggattggcg aataaaaaaa tgaaggggat
     1201 gaaacaaatg gatttagcct ctactattcg taatattcct gatttcccaa aagcaggaat
     1261 tcaatttaaa gatataacaa ctttattaaa aaataaagat gcttttcgcc aagctgtcga
     1321 ttcactgttt catctttttg atagtaaaga agtggatatc gtggttggaa tcgaagcccg
     1381 aggttttatt ttaggttcgg ttgttgccta tcttttgaat tgtggattcg taccggttcg
     1441 aaaaaaggga aagcttcctg caacgattag gtcaaaaacc tatgatttgg aatacgggaa
     1501 tgctaccctg gaaatgcatg tagatgccat agaaaaaaat caaaaagtgg tcattatcga
     1561 tgatttattg gcaaccgggg gaacagttca agctgtatgt gagatggttg aagaagccgg
     1621 tggagaaatc gttggtattg catttttaat cgagttagag tctttacagg ggagaaaagc
     1681 tatcacaaag tacccagttt attctttggt taaattagac tgaacaattt tctccttctc
     1741 ccttgatggg agaagattgg gatgagagtg aaaatcctta tctaaatccg tcattgcgag
     1801 aagtccaacc gtacttagat tgtactctta gattaacagt tctcctccac tggtggggta
     1861 tctatcgccc agtgataggg taagtctgga tttattaaat atttgttttc aagataatta
     1921 tctaagaagc tcataccttt ctttctgaag aaaaagggag ggaaatatgc ttcgcaatta
     1981 cgaagtaaat agtatatttt caaaaactat aacccttgaa tcgcttcaag aaaaaatcga
     2041 ttcctataac cccaatagcg atcgagaact gataaaaaaa gcttatgatt tctcggtaga
     2101 cgctcattca ggacaaagtc ggctgtcggg tgaaccctat attatccatc cattatcagt
     2161 agcccatatt gtcgctgacc ttcgcatgga tgatagtacg attgctgctg ctttacttca
     2221 cgatgtatta gaagacacta aaaacaccga agctcaactt aaagaaactt ttggaaaaga
     2281 aataactctg ctggtaaaag gagttagcaa gctgaacgtc ggctttcact ttagttctaa
     2341 agaagaagag cagattgaaa attatcgacg cctctttgta gctatggccg aagatatacg
     2401 ggttattata ataaaattag ccgatcgcct tcataatatg cgtaccctga aataccaata
     2461 tcgagataaa aaagagttga tagccagaga aactttggaa atattcgctc caatcgctca
     2521 tcgcctgggg attggtatga ttcaaaccga attggaaaat ttaagtcttt attttcttga
     2581 cccggaatct tattgcagca tccaacaagg gatagagaaa attagagatc accgagaaaa
     2641 acaaatccaa gaggcgacgg aaaagctctc tcaaagaatt caacaagcca atttggtggt
     2701 tgaaatccaa agccgtctga aaaatattta tagtatttat ttaaaaatca atcgtcaaaa
     2761 cgtaagtctt gaagatcttc atgatttgat tgcccttcga gctataacca ataccgtcga
     2821 agagtgctat gcagtattag gactggtgca ttcgctttgg gtgccggtag cagggagatt
     2881 taaagattat attgctcttc ctaaatcgaa catgtatcaa tctcttcata ccacagtaat
     2941 cggtccaggc ggagttccaa tggaaattca aatccgaacc agagagatgc atcatgtggc
     3001 tgagtatggt attgctgccc attggagata taaagaaggg aaatccgctg attcagctga
     3061 tgcctttaac gaacgtatgg cctggttaag gcaaatattg gaatggcagc aagatctaag
     3121 gactacccat gaatttgtcg aaagtcttaa aatagacctt tttgatgatg aagtatatgt
     3181 gtttacgccc aaaggagatt taaaaaaact cccgaaagga tcaagtccta tcgattttgc
     3241 ttatttaatc cacactgaag tcggaaataa ttgcattggt gcgcgggtga ataagcaaat
     3301 tgttcctctg gactacgaac tcaaaagtgg agatattgta gaaattattt cttcccgttc
     3361 ttcttccgga cccagtttag actggttgag aatcgccaaa acccctgctg ctcgaaataa
     3421 aatccgggca tttctgcgca aaaagaatcg agatgccaat cgagagcggg gaaaattatt
     3481 tatggagagg gaattggaga aatttaccct tacctcagaa gaacagaatt tggtgaatga
     3541 taaaattacc gaatttatgg aagccaataa ttataaaaca atcgacgatc tccatgttat
     3601 aatcggagaa ggacggctta ccatccgtca atttattgga aaagttgttc cttttgaaat
     3661 tcgaaaacgt tttcaaaccc aacgaaaagc agaaactaaa aaaaccgctc aaactagtgg
     3721 agacggagtg atcatccagg gcgcaaccgg tttagcggtg aagttagctc gttgttgtac
     3781 gccaattcct ggcgataaaa ttatcgggta tgtaaccttg gggaagggta tcaccgtcca
     3841 tcgcttggat tgtcataatt taaccaggtt gacccaagat gaagaacgat tggttgaggc
     3901 ggaatgggat gaaaggtacg gccgttttta cgatgccgga ttggttattg acgccctgga
     3961 tcgtccaaaa ctcttggtgg acattaccaa tactgttgcc aattatggtg taagtattcg
     4021 atcggttcat gctcattcca tgatgaatga tgcccggata tatttagtta tttcggtaaa
     4081 aaataaagtt gagcttgata acctgtttcg tgaaataaaa agggtctcca atgtcagaac
     4141 tgtgcgaaga ggaggaatat ttggtttgcg gtgagagcgc ttattcaaag ggtgaaccag
     4201 gcacgggttt taattgatgg caagatagtt ggtgagatcg ggaaaggaat ggtggtattt
     4261 ttaggagttg ggaaaaacga tagctcgtca gacattgatt ttatggtgag gaaaatccct
     4321 cagcttcgaa tattcgatga tgctcaaggt aaaatgaata tcagtttgaa tgaaatacaa
     4381 ggtgagattc ttctgatatc ccaattcact ctttacggga attgtcgaag aggacttcgc
     4441 ccaagttatg atgaggcggc aacgccacga gaagcccaaa atttttataa tcaaatagct
     4501 caaaagctac gtgatcagga tctaaccata gctgaaggag tcttcggagc gatgatgcaa
     4561 gttgaattgt gtaatgatgg tccggtgacg attttgcttg acagcaagga aaggagacag
     4621 gcatgagtaa caaggtgaaa gtcgcagtct atacattggg tgatttaatg accaattgtt
     4681 actgtctttg tgattgtaat gaagctctcc ttattgaccc tggaagccct tcccaagaac
     4741 tagttgagtt tttaacttct cgtaaattga atttaaggta tattatcaat actcacggac
     4801 atatcgatca tattcgggga aatgattttt ttaaggatta tttttcctct gtcaaaatta
     4861 taatttatga aactgatatg gcatatctca accaccccaa tctcaaccta tcgacagatt
     4921 tttctcaacc ttatcgatca ccagagccag atattcttat tcgagggaaa aaagaacctt
     4981 ttgaattatt tgatgaagag gtttactttt atcatagccc aggccacaca cctggaagca
     5041 tgtgcttatc cttcccaaat cagaaatggt tattttgcgg tgatacactt tttgctggat
     5101 cgataggaag aaccgatctt cccggtggtt cgtttcagga aattcttgag tctctgacca
     5161 tgattatgca atcttttgac gatgaaacta gagtctttcc cggtcacggt ccagaaacaa
     5221 ctattggaaa tgagaaaaaa atgaatccgt atatccttga atacctgaa
//
LOCUS       DAOSOL010000072         5240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_15882_length_5240_cov_3.882932, whole genome shotgun sequence.
ACCESSION   DAOSOL010000072 DAOSOL010000000
VERSION     DAOSOL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15882_length_5240_cov_3.882932"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..2116)
                     /gene="fusA"
                     /locus_tag="PK171_02725"
     CDS             complement(59..2116)
                     /gene="fusA"
                     /locus_tag="PK171_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT05151.1"
                     /translation="MNIERVRNIGIMAHIDAGKTTVSERILFFTRKIHRVGEVHEGMA
                     TMDFLPQEQERGITISSAVTTCFWNNARINIIDTPGHVDFTVEVERSLRVLDGAVAVF
                     CGVGGVEPQSETVWHQADHYRIPRIAFINKLDRVGADFFAVVNAIRKKLNANAHPIQI
                     PIGKESDFIGVIDLIEMKAFTYRKDSDGMEFDWVDVPDSYRKEALIYRDKLLEAISEI
                     DENIMQKYLDGDEINPQEIRQAIRKGTTTGKFIPVLGGSALRNKCIQPLLDAVVWYLP
                     SPLDLPPVEGKNPKTNEIEKRLPMKKEPFSGLVFKISMDPHAGKISFFRVYSGQLQSG
                     TYVYNSNKGAKERVSRILIIHANRREDVQAVKAGDLCGIVGLRNAATGDTLCEERYPI
                     VLETLVFPEPVIEVAIEPKTRADQEKLGLALSKLAEEDPTFKVKTDEETGQTLISGMG
                     ELHLEIIIDRLLREFKVEANVGRPQVAYRETIRKSGRGEGRYVRQTGGRGQYGHVVLE
                     IEPCDDNFVFEDKIVGGAVPREYIPSVQAGVKESLDNGVIAGYPVMNIRVRLIDGSYH
                     PVDSSDIAFKIAGSLALRDAIKKSQPILLEPVMKVQIITPKDYLGDVIGDMSARRGKI
                     EGIESKGELQVINAQAPLASLFGYATALRSVTQGRATYTMQFSHYDEVPENMMKEIID
                     KLS"
     gene            complement(2173..2646)
                     /gene="rpsG"
                     /locus_tag="PK171_02730"
     CDS             complement(2173..2646)
                     /gene="rpsG"
                     /locus_tag="PK171_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOT05152.1"
                     /translation="MPRKGPVSKREIQPDPVYNSVDVARLMNKVMIKGKKSVSEQIVY
                     GAFEIVREKSKRDPLEVFSQALNNVMPLVEVRARRVGGANYQVPMEVRPERSKSLGIR
                     WLVNYSRQRAGRTMTEKLANEIIDAANGAGASSKKREDTHRMAEANKAFAHYRWF"
     gene            complement(2662..3036)
                     /gene="rpsL"
                     /locus_tag="PK171_02735"
     CDS             complement(2662..3036)
                     /gene="rpsL"
                     /locus_tag="PK171_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOT05153.1"
                     /translation="MPTINQLVRKGRKQPKKKSSAPALKCSPQKRGVCTRVWTITPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSLVLIRGGRVKDLPGVRYHIIRGTL
                     DTAGVENRKQSRSKYGAKRPKS"
     gene            complement(3512..3709)
                     /locus_tag="PK171_02740"
                     /pseudo
     CDS             complement(3512..3709)
                     /locus_tag="PK171_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4814..5209)
                     /locus_tag="PK171_02745"
                     /pseudo
     CDS             complement(4814..5209)
                     /locus_tag="PK171_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
BASE COUNT         1364 a         1261 c          907 g         1708 t
ORIGIN      
        1 ttgacgtggg gtttagatcg ttcaaacttt tgtttcgcca taggtatcct cctctttctt
       61 aactcaattt atcaataatt tctttcatca tattctcagg aacttcatca taatgagaaa
      121 actgcatagt ataagtagcc ctaccctggg taaccgaacg aagggctgtc gcataaccaa
      181 aaagcgaagc taaaggagcc tgggcattga taacctgtaa ttctcctttg ctttcgatac
      241 cttcaatttt accccttcgt gcactcatat ccccaataac atcacccaga tagtctttgg
      301 gagtaatgat ttgcaccttc ataaccggtt ccaaaagtat cggttgagat ttttttatgg
      361 catctcgcaa ggccagagat ccagcaatct taaaggcaat atcggaagag tctaccgggt
      421 gataggaacc atcaattaaa cgaacccgaa tgttcataac cgggtaacca gcaataactc
      481 cattatccag tgattctttt actccggctt gaaccgaagg aatatattct cttggtactg
      541 ccccaccaac aattttatct tcaaaaacaa aattatcatc acaaggttcg atctctaaaa
      601 caacgtgtcc gtactgtcct cttccaccgg tttgacgaac ataacgtccc tctcctcgcc
      661 cacttttccg gattgtttct cgataagcaa cttgcggtcg acccacattg gcttcgactt
      721 taaactctcg taaaagacga tcgatgatga tttcaagatg taactccccc attcccgaaa
      781 tcaacgtttg accggtttct tcatcagttt taactttaaa agttggatct tcttcggcta
      841 attttgataa ggctaaacca agtttttctt gatcggcacg agttttgggc tctattgcta
      901 cttcgattac tggttcagga aacaccaacg tttctaaaac aataggataa cgttcctcac
      961 agagggtatc accggtggct gcatttcgca atcctacaat accacacaag tctccagctt
     1021 ttaccgcttg aacatcttcc cgtcgattcg catgaatgat caaaattcga cttactcgtt
     1081 cctttgctcc tttattactg ttgtatacat aagtaccgct ttgcagttga ccagaataga
     1141 cacggaaaaa agaaattttc cccgcgtggg gatccatgct tattttaaaa accaaacccg
     1201 aaaaaggctc tttcttcatg ggaagccgct tttcgatttc attggttttc ggattttttc
     1261 cttcaactgg cggcagatcg agtggggatg gaagatacca aaccactgca tctaaaagag
     1321 gctgaataca tttatttcga agagccgagc cgcctaatac tggtatgaat ttcccggtgg
     1381 ttgttccttt acgaatagct tgacgtattt cttggggatt tatctcatct ccatccagat
     1441 atttttgcat gatgttctca tcgatctccg atattgcttc caggagctta tctcgataga
     1501 tcaaagcttc tttccgataa gaatctggaa catctaccca gtcaaattcc atcccatcac
     1561 tgtcttttct atatgtaaag gctttcattt cgatcagatc aataacacca ataaaatcac
     1621 tttcttttcc gatagggatt tgaatcggat gagcgttggc attcaatttt ttacggatag
     1681 cattgacaac cgcaaaaaaa tcagcaccaa cccgatctaa tttattaata aatgctatcc
     1741 gaggaatccg gtaatggtct gcttggtgcc aaactgtttc tgattgtggc tctacaccgc
     1801 caactccaca aaacaccgca actgccccat ctaacacccg gaggcttctc tctacttcaa
     1861 cagtaaagtc cacgtggcct ggcgtatcga taatatttat ccgagcatta ttccaaaaac
     1921 aagtggtcac cgctgagctg atggtgattc ctctttcttg ctcctgggga agaaaatcca
     1981 tagttgccat accctcatgg acttctccta cccggtgaat ttttcgggta aaaaacaata
     2041 tacgctcact gaccgttgtt tttccggcat caatatgagc cataatccct atgtttctaa
     2101 ctctttctat attcaattgt tcgaattctt gtccctgatt cagattgcta ttcaaaaaat
     2161 gaagcccctt tcttaaaacc accgataatg agcaaatgct ttattggctt ctgccatgcg
     2221 atgagtatct tctcttttct tagacgatgc ccccgctccg ttagcagcat caataatttc
     2281 attggctaac ttctcagtca tagttcttcc tgctcgttgt cggctataat taaccagcca
     2341 tcgaatacca aggcttttgc tccgttcggg acgaacttcc atcggcacct ggtagttggc
     2401 tccaccaacc cgtcgtgctc tcacttcaac caatggcatt acattattca gtgcttgaga
     2461 aaatacttct aatggatcac gttttgattt ctcacgaact atctcaaaag caccataaac
     2521 aatttgttct gaaacactct tttttccttt tatcatgacc ttgttcatca accgtgcaac
     2581 atcaacactg ttatatactg gatctggttg gatttctctt tttgaaaccg gaccttttct
     2641 cggcaaaacc cttcacgctc cttatgattt cggtcttttc gctccgtatt ttgaacggct
     2701 ttgtttgcga ttctcaacac cagccgtatc aagcgttcca cgaataatat ggtatctcac
     2761 gccgggaaga tctttcacac gaccaccacg gataaggacc aaggaatgtt cttgaagatt
     2821 atgtcctatt ccaggaatat aggcggttac ttcaattcca ttggtcaaac gcacacgagc
     2881 aaccttccgc aatgccgagt tgggtttctt aggtgttatt gtccaaactc gggtgcacac
     2941 tcctcgtttt tgaggagaac atttcaaagc aggagcactg cttttcttct taggctgttt
     3001 tctgcctttt cgaaccaact ggtttatggt tggcatatcg ttcctccttc ttaatatctt
     3061 gctctcatga gttgaatata tttatttcct tatatttttt cctatttctt tatactatcg
     3121 gactttacaa tagtacccct aagtgcaacg gagcaatttt atcagcatta aaattgaatg
     3181 tcaactggga gtttcagtat ttgattaaaa aaagtgggag tttatcgctc ccactttttt
     3241 ctcctttttt ttaatcgtca aaattactct cttcttcatc tttatcgcta tcgatttgac
     3301 tttcaaaatg gccaaactct tcaggatcct cgtctatatc ctcatcttca gttttgtttt
     3361 gatgaggatg aagaatttct tcgatatcga tttcttcttc ataattcatc agttcacgaa
     3421 gatcaacact ttggtctccg tgaaaagcca attcgcttga atcttccatg tgttcttcgg
     3481 tctctaactt caccttccga tattctggaa aaccagttcc agctgggata atcttaccga
     3541 ttattacatt ttcttttaat ccaaccagtt catcaacttt tccttttatt gctgaatccg
     3601 ccaaaacgtg agtcgtctgt tggaaagaag atgccgataa aaagctctca gtggctaaag
     3661 aggctttggt gattcccaga acgactggtc tcccggttgc tacctgtttg gtacctgcta
     3721 aatagactaa attttcaacc aaacttctgg tttcagtttc ggcgtccgct tctcgtatca
     3781 ataaatactc gacattatcg tcagcaattt tgattactcc atcacgatcg atagtttgtc
     3841 ctgctttgat cagtatagag ccattctctt ctatggaaat atcttgtgct gcgactttcc
     3901 ctacgacttc tcgttctaaa agttcactta atcgtccttc tattgaaaag aaacgtccat
     3961 cgcccagttt gacttcttta acatcatcca tgaccaaacg ccgtgccact gcccggctta
     4021 tttgctgacc agaggctatg atcaaatcac cgcttttcgg actaatgatg tccttttcca
     4081 cttctttccc aagaatctcc tcacgtaatt ctcgaattaa actttcttta atttggaagg
     4141 tctcaatatt tttccagatt acaatccgat cgatattatg accttcaatc aggtcttgat
     4201 gatgggcttc aacttttgta ttggcggtga gaattacttc tccggttttc aaatcggtca
     4261 catcttcagc taaaattgca cctttcagtt tatcccaggt tgacttctct cgggttcgca
     4321 gaataaccgg acctaagtca gctatatgtt caaccttctg ctcgctaata acatcaccgg
     4381 ctttgaccaa aaccgttcca ttttgatcaa gaatgtcatc gacgctcact tggtctttca
     4441 attcagcttt taacgaccgg tagcctcgaa tcattttgta aggtgtctga tctttattaa
     4501 ctatataaaa aggcttgcaa tcggtagata aaataaccct tagcttctct tcgtcaattg
     4561 cttctccttt ttgtacaatc gcttcacccg aaatatcaaa agccggttct cgtaaggtga
     4621 acccttcaaa aagcgagagt gctttttcta aaaccaatcg gttttcttca ttaattcgac
     4681 tattttcttg ctcaaaatct tcaatatgaa ccaactcacc ggagaggagt tcggtatcac
     4741 cggaattctc aaccataacc ttattcagac gagcaatttg tcgaacaatg gtttcgatgt
     4801 gtttatcgtt gatgtttaca ccttgtgata ggtaaaccga ttgaatttct tctaccagat
     4861 gactttgaac ctctcttatt cccttgatac gaaggatatc acgaggatta atcgggccta
     4921 aggtcaaacg atcacccgct ttaacctgag cattttcatt aaccttaatc cgaatatcag
     4981 gtggaactaa ataagcccgg gccgattgat cggcttcatc gtcatcagga tgaacaataa
     5041 tttttcttcg gtttccaatg gtttcaatct tttctacttc tccatcgaca tcggatagaa
     5101 tagcggcttt ttttggtttc cgaacttcaa atagctgctc tactcgtggg agaccttggg
     5161 taatgtcttc ccccgcaatt cgaatccctc cggtatgaaa ggttctcata gttagctggg
     5221 tgcctggttc gccgattgat
//
LOCUS       DAOSOL010000073         5199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_16071_length_5199_cov_4.026633, whole genome shotgun sequence.
ACCESSION   DAOSOL010000073 DAOSOL010000000
VERSION     DAOSOL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16071_length_5199_cov_4.026633"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..418
                     /gene="rpsR"
                     /locus_tag="PK171_02750"
     CDS             173..418
                     /gene="rpsR"
                     /locus_tag="PK171_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOT05154.1"
                     /translation="MRSERDSGKRFFRRSRKKNCIFCVEKGPIDYKDINRLGRFISER
                     GKIVPKRVTSVCAKHQRELSIAIKRAREIGLLPYSLD"
     gene            440..1348
                     /locus_tag="PK171_02755"
     CDS             440..1348
                     /locus_tag="PK171_02755"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HOT05155.1"
                     /translation="MRKLRPTVEGAFLAALTVVLYLSSVYLPLFGFFLSFFCPLPVLF
                     LVVRWDLRTGALAAGVATLLVAFTGLIPALICISYTLIGIFLGYAIKKKYSFFEVIGF
                     GSLVSLLSKIALIGFALLITGINPIVENLQIMEEAFQRTSQVFGGIGEENFQQIVTLI
                     NLALPAILVVASILDTTINFFLGSWVGKKIGIPFPEYPAFRNWKFPVSVFWMFILSWM
                     FVLFGGETIYGRIGLNLQIVTQSLFIVQGVAIVYYFLSRYIQSRGVKIIILLFVVFQP
                     IFSTILSWLGVLDTWFDFRKMSSPKG"
     gene            1428..1916
                     /gene="rplI"
                     /locus_tag="PK171_02760"
     CDS             1428..1916
                     /gene="rplI"
                     /locus_tag="PK171_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT05156.1"
                     /translation="MQLILLQKVENLGDEGDVIEVKEGYARNYLLPKKLAIKATKGSL
                     TQIDILKRKKKQREEKELDAIRQLQKKVDGLRLEFNRKAGEKGKLYGSVTSKEIADKI
                     GEILELELDRKFIDLPEPIKDLGETEVKLHFGKGIYGSVQVQIIPEESVQPDVSKEVK
                     ES"
     gene            1919..3262
                     /gene="dnaB"
                     /locus_tag="PK171_02765"
     CDS             1919..3262
                     /gene="dnaB"
                     /locus_tag="PK171_02765"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT05157.1"
                     /translation="MERVPPQSLEAEQATLGSMLLERNALLKALEILRPEDYYYDNHR
                     IIFTAIGELFEEDKACDIVTLAEKLRAKNQLETVGGLEYLALLTNLVPTAANVEYYAH
                     IVEEKAIIRFIIQICTQIIKESYENQIDATELLDRSQHMILQLSQSRIKSDFVALKTV
                     INEAFDRIEDLYHRDEHITGIPTGFVDLDNITAGFHPSELIVIAARPGMGKTSFCLNI
                     AQHVGINKKIPVAFFSLEMSRDHIAQRMLCGEAGIDASRVRRGLINEKDWPILASAAG
                     RLASASIYIDDTPGITVLELRAKARRLKIEKNLGLVIVDYLQLMSGHIRTENRQQEIS
                     EISRSLKAMARELDVPVVAVSQLSRAVEQRNPKRPQLSDLRESGAIEQDADLVMFIYR
                     EEYYNPNTQSKKNIAEVIVAKQRNGPVGAVELLFRNAFTKFENLSRRSYNPGVEE"
     gene            3340..4113
                     /locus_tag="PK171_02770"
     CDS             3340..4113
                     /locus_tag="PK171_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT05158.1"
                     /translation="MKKHLVIFLVIMVGMIGLSSLAMASTLDDIKARGTIIIGTDATY
                     PPMEYHDEAGNMIGFDIDLGNAIAELLGVKAEFIDTAWDGIFPALDSKKFDIIMSSTS
                     ITEERLKSKDMSDPYYQTSQAIAIRKDDDSIKGPEDLVGKVVAVQIGTTGDFAVSEIE
                     GIKEIKRFDTTDKAYMEVMNKRADAVVNDYSEVSFRMTLLPDMKVAATFKEGEDIYGV
                     TMRKGETELLAAINEAIAKLKESGKYQEIHDKWFTVPGN"
     gene            4410..4571
                     /locus_tag="PK171_02775"
     CDS             4410..4571
                     /locus_tag="PK171_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05159.1"
                     /translation="MQFDLSALIKAIPALLYGASVTLRITVLSIAMGLVIVSTYCRDI
                     IVFPSVISF"
     gene            complement(4623..>5199)
                     /locus_tag="PK171_02780"
     CDS             complement(4623..>5199)
                     /locus_tag="PK171_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0236 family protein"
                     /protein_id="HOT05160.1"
                     /translation="IFVLDRFHLEKYLQDGLRKDPTTLFQIREALGRGDQEEVKILLK
                     NARKATQEEKEIEKLQVLRRYLLNNWEGIITYREYPKLPLGGSAEGHVSHILSARLSS
                     RPMGWSKKGADHMSYLRAMKANGHSAKAFYLKTHRQNLIPFTLDQDTITKEREKGREV
                     MREIYDNIPILKGPVSNFSQVLRSLTKDSLP"
BASE COUNT         1615 a          848 c         1128 g         1608 t
ORIGIN      
        1 gcagccagtc aatcgaaaaa gggagttggg cttattgtga gggatcgtta caaatacgca
       61 cctttggtga agtgggtgct cagaaaaaaa cgacagttga agtgattctt aaagatattt
      121 ttcgacttca gcaataaaaa gaatttaaaa tattaagaga ggttagatac tgatgagatc
      181 tgagcgagat tcaggaaaaa gattttttag acgttcaaga aagaaaaatt gtattttctg
      241 tgttgaaaaa ggtccaatcg attataagga tatcaatcgt ttgggacgtt ttattagtga
      301 aagagggaag attgttccga aaagagttac cagtgtttgt gctaaacatc aaagggaact
      361 ttcaatagcc atcaaaagag ctcgggaaat tggtttactt ccctattcgc ttgattaaat
      421 tcgactcgat tgaaaaaaaa tgaggaaact cagacctact gttgagggag ctttcttggc
      481 tgccttaaca gtagttttat atttatcaag tgtttaccta ccattattcg gtttcttttt
      541 aagttttttt tgtccattac cggtattgtt tttagtagtt cgctgggatt taagaactgg
      601 tgctttagct gccggtgtag ccactctatt agtcgctttt accggtttaa ttccagccct
      661 aatctgtatt agttatactc tgattggaat ttttttggga tatgcaatca aaaagaaata
      721 ttcttttttt gaagtaatcg gtttcggcag tttggtatcg cttttatcta agattgcatt
      781 aatcggtttt gcactcctca taaccggaat caacccgatt gttgaaaacc ttcaaatcat
      841 ggaggaagcc tttcaaagga cttctcaagt gtttggagga attggggagg aaaactttca
      901 gcaaatagta accttgataa accttgctct tccggcaata ttagtggtag cttccatact
      961 tgataccacc attaattttt ttttgggatc atgggtaggg aagaaaatcg gaataccctt
     1021 tccagaatat ccagcctttc ggaactggaa attcccggtt tcggtttttt ggatgtttat
     1081 cctgagttgg atgtttgtct tgtttggcgg ggaaaccatt tacggaagaa tcggtctcaa
     1141 cttacagatt gtaacgcaat ctctttttat cgttcaggga gttgccattg tatattactt
     1201 tctgagtcgc tatattcaat ctcgtggtgt aaaaataata attcttttat ttgtggtatt
     1261 ccagccaata tttagtacga ttttaagctg gttaggagtt ctcgatacct ggtttgattt
     1321 tagaaaaatg agctcaccaa agggataatg cgaaggatca gttctcatgc tgttttgggc
     1381 taataagttt gaggatagag gtttctttgc agaaaaggag tgatattatg caacttatac
     1441 ttttacaaaa agtggaaaac ctcggtgatg aaggtgatgt tatcgaggta aaagaaggat
     1501 acgctagaaa ttatctcctt ccaaagaaat tggcgattaa agcgaccaag gggagtttga
     1561 ctcaaattga tattttgaag cgcaagaaaa agcaaagaga agaaaaagag ttagatgcta
     1621 ttcgtcagct tcaaaaaaag gtagacggat tacgtcttga atttaataga aaagctggag
     1681 aaaaaggaaa actttacggt tcagtaactt ccaaagaaat tgctgataaa attggagaaa
     1741 tattagaatt agaattggat cgtaaattta ttgatcttcc agaaccaatc aaagacctag
     1801 gtgaaaccga agttaaactc cattttggca aaggtattta tgggtcagta caggttcaga
     1861 ttatccccga agaaagtgtt caaccggatg tttcaaaaga ggtgaaagaa tcctgagtat
     1921 agaaagagta cctccccaaa gtctcgaagc ggaacaagcg accttgggtt caatgttatt
     1981 ggagaggaac gctcttctta aagcattgga aattcttcga ccggaagatt attattatga
     2041 taaccatcgg atcattttta ctgcaattgg cgagcttttt gaagaagata aagcctgtga
     2101 cattgttact ctggctgaaa agctcagagc gaaaaatcaa ctggagacgg tgggaggtct
     2161 tgaatattta gcacttttaa ccaatttagt tccaactgca gcgaatgttg aatattatgc
     2221 tcatatcgtt gaagagaaag ctattattcg gtttataatt caaatttgta cccagataat
     2281 caaagaaagt tatgaaaatc aaatcgatgc tactgaatta cttgatcgat cacagcatat
     2341 gattttgcag ttatcgcaaa gccggataaa aagtgatttt gttgctctaa aaacagtcat
     2401 caatgaagct tttgatcgta ttgaggacct ctatcatcgt gatgagcata taactggcat
     2461 tccaaccggt tttgttgatt tggacaatat tacggctggg tttcatcctt ccgagctgat
     2521 tgtaattgca gccagacctg gaatgggaaa aaccagtttt tgcctgaaca tagctcagca
     2581 tgtgggcatt aataaaaaga taccagtggc atttttcagt cttgaaatgt cacgagatca
     2641 cattgcccaa agaatgcttt gtggagaggc cggaatcgat gcatctcggg tgagaagagg
     2701 gcttattaat gaaaaagatt ggccgatatt agcttcagca gctggacggt tagcaagcgc
     2761 ctcaatatat attgacgata caccaggaat taccgttctt gaattgcgtg cgaaagccag
     2821 gagactaaag atagaaaaaa atttgggctt agtcatcgtt gattatttac aattaatgag
     2881 tggccatata cgaaccgaga atcgacaaca agaaatttca gaaatttcgc gatctttaaa
     2941 agccatggct cgggaacttg atgtaccagt tgttgctgtt tctcaattaa gcagagcagt
     3001 ggagcagaga aaccccaagc ggccccagct ttcggacctg cgtgaaagtg gagcgattga
     3061 gcaggatgcc gacttggtaa tgtttatata tcgggaagag tattataatc ccaataccca
     3121 gagtaaaaaa aatattgctg aagttatcgt agccaaacaa agaaatggtc cggttggtgc
     3181 tgtcgagctt ctttttagga atgcttttac gaaatttgaa aacttatcac gtcgtagcta
     3241 taatccaggg gtggaagaat aaagtatttg gcagttatct tttcattgac ataaaattct
     3301 tatcaatgat attattttca aaaattggga gggattcatt tgaaaaaaca cttagtaatt
     3361 tttttagtca ttatggttgg aatgataggt ttgagttcgt tggctatggc gtcgaccctg
     3421 gatgatataa aagcacgggg gacaatcatt attgggacgg atgcgactta tccaccgatg
     3481 gaatatcatg atgaagcagg aaatatgatt ggttttgata ttgaccttgg caacgccatt
     3541 gccgagttgt tgggagtcaa agcggagttt attgatacag cttgggatgg gatatttcca
     3601 gctttggatt cgaaaaagtt tgatatcatc atgtcttcga cttcaataac tgaagagcgg
     3661 ttaaagtcta aagacatgag tgacccttat tatcaaacat ctcaagcaat tgcgattcga
     3721 aaagatgatg actcgataaa agggccagaa gatttagttg ggaaagtagt tgcggttcaa
     3781 attggaacta ctggagattt tgctgtaagc gaaattgaag gtataaaaga aattaaacgt
     3841 tttgatacca ccgataaagc ctatatggaa gttatgaata aaagagctga tgctgtcgtg
     3901 aatgattatt ctgaagtatc attccggatg acgcttcttc ctgatatgaa agttgctgct
     3961 acattcaaag aaggagaaga tatttacggg gtaacgatgc gtaaaggtga aaccgaatta
     4021 ttagcagcca tcaatgaagc aattgctaaa ttaaaagaat cgggtaaata tcaggaaatt
     4081 catgacaaat ggtttactgt tccgggaaat tagaagagca gaaacagttt tcataaaaaa
     4141 tttttaatgg gcgctatgaa atagcgccca ttttttcggt atttgaaaaa atattgatta
     4201 aaataagtgt tttctggtgg tcacataact cttcgattgt aaaaaacaga aaagatagca
     4261 ttttttcttt tttactgtag gcaggagttg gatgcagttt aggcttagat ttaaaaaaat
     4321 gagttttacc aataaatagt aaaaaaacat tacccaagtg ggaatcaaac cacgatcaaa
     4381 aatgttcgaa gcgaaaggag gtaccatggt tgcaatttga cctatcagca ctcattaaag
     4441 ctattcctgc tttactttat ggagcatcgg ttaccttaag aattacagtt ttatcaatcg
     4501 ctatgggttt agtgatagtg tcaacctatt gtagggatat tattgttttt ccctctgtta
     4561 ttagcttttg agatcctctc cattcagtat gagaaagtat cattttaaat taaaacaatt
     4621 aatcaaggta aagagtcttt cgttaaactt ctaagcacct gagagaagtt ggaaactggt
     4681 cctttcagga tagggatatt atcatagatc tctcgcataa cttctctccc tttttctctt
     4741 tctttggtaa tggtgtcttg atccagagta aagggaataa ggttttggcg atgcgttttc
     4801 aagtagaaag ctttagccga atggccgttt gctttcatcg ctcggagata actcatatga
     4861 tcagcacctt tcttgctcca tcccatcggt cggctggaaa gccgagctga gaggatatga
     4921 ctcacatgac cttcggcgct tcctcctagg gggagcttag gatattctcg ataagtgata
     4981 atcccttccc agttattcaa gaggtacctt ctcaaaactt ggagcttctc gatctctttt
     5041 tcttcctggg ttgcctttct ggcattcttc aagagaattt tgacttcttc ttgatctcct
     5101 cttcctagag cttcccttat ctggaataaa gtagtcgggt cttttctcaa cccgtcctga
     5161 agatattttt ctaaatggaa acgatctaaa acaaaaata
//
LOCUS       DAOSOL010000074         5125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_16416_length_5125_cov_4.099014, whole genome shotgun sequence.
ACCESSION   DAOSOL010000074 DAOSOL010000000
VERSION     DAOSOL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16416_length_5125_cov_4.099014"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..642)
                     /locus_tag="PK171_02785"
     CDS             complement(22..642)
                     /locus_tag="PK171_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT05161.1"
                     /translation="MKYTEYIWDFDGTLFDTYPVMVKAFQRALRELGIEEKPDTLLYF
                     MKKSIAETIDYYQDRYLLGELFFKRYLEIRQSYSLDEQPPISGAVKVCCQIVQQGGRN
                     FIVSHRESPSLYAFLDHYGMRQLFTEIITGNDGYERKPNPEAFNYLVKKYQLKKGSTL
                     AIGDRILDIQAGREAGIKTVFINLNDGLECDFADFKINSLEEALSL"
     gene            complement(647..1342)
                     /locus_tag="PK171_02790"
     CDS             complement(647..1342)
                     /locus_tag="PK171_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOT05162.1"
                     /translation="MIELHKVTYEFSQGWPRKQHQIVLSNISVKLYMGKTFGLMGHSG
                     TGKTTLGKMVAGLIKPFSGTIFFHGKDISRMKPPDWLFFRRNVQMLFQDPQGSLNPKK
                     RIDASLRDVLNITKVPFNQQKEAIEDVLKTVGLSTDFLLRYPAQLSGGQNQRISLARI
                     LLLKPEFIVLDEPTSALDISVQAQILFLLRELQKNHGLGYLFISHDPEVIQYMSHTIG
                     ILKNGNLDIVPEG"
     gene            complement(1335..2273)
                     /locus_tag="PK171_02795"
     CDS             complement(1335..2273)
                     /locus_tag="PK171_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05163.1"
                     /translation="MSLLTVERLNVVFTTHQGRVKAVKNVSITIPHQETLALIGETGC
                     GKSVIAQSILRLLPGNAQINGRILFQGRDLLLVDEKTMASIRGKEIAIIFQNPSLALN
                     PVYSIGWQVGEPFRIHQGVDRNQSIQESVRLLSYMKFDKPESAVKMYPFEFSGGMNQR
                     VLIASALALHPNLIIADEPTQGLDRTLIKQIIDQLDRARKIHSSSMLLITHDLTVARS
                     ISDWIMVMYAGEIVEQVPTLDFFQSPFHPYSQGLLRSLPENGFHPIPGQSPSMIEDLQ
                     GCQFHPRCQWAREQCHREKPGLMSVNERLVRCFLYD"
     gene            complement(2254..3105)
                     /locus_tag="PK171_02800"
     CDS             complement(2254..3105)
                     /locus_tag="PK171_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05164.1"
                     /translation="MKDLIFQRKKYFIRIGGFLFILFLLVAIFANFIAPFQLKDRFDP
                     FLPLGLKHLLGTNDIGNDLFTEFIYGSRITLLVGLLTGVFSVSIGVLIGLFAGYHGGF
                     IDELLMGITDVILMIPRIPLIIVLSAFLRPSVGLMILVMSILWWTSTARVIRSRTMQI
                     RSMGFVESARCLGFSGWHILFYEILPNIVMIIIPEFLITVASAMISEASLSFLGLGDP
                     SLKSWGMMIHHAFQRGGFLNGMWWWYIPPGMSITLFVLSMVFLSFAFEETEIGMGEEV
                     AIVPFNR"
     gene            complement(3102..4049)
                     /locus_tag="PK171_02805"
     CDS             complement(3102..4049)
                     /locus_tag="PK171_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05165.1"
                     /translation="MVFPAKKILRYVFALFFIITLNFFLPRAMPGDPVINLLGEDFIA
                     GKEAVEKIREELGLNRPILVQYANYWKNLFQFDLGYSYHFRARVSSLIISRLKWTFIL
                     VAPSLILGTIIGALAGSLAGWKRKSKGNTFSFFFFLFIYCSPPFFISLLFLYFFGFRL
                     GLFPLKGFYTTGNWIDILKHLFLPVLIMTLFSASRNYMVMRGSVLQEKEKLYVWYARA
                     KGHLEDSILFQHVFKNASLPVITLFALDFGFLFAGALFIEIVFSLNGMGTLIYQSILS
                     RDYPVLQGTFLIISFMVIIANYLTDWIYNWIDPRVRANR"
     gene            complement(4474..>5125)
                     /locus_tag="PK171_02810"
     CDS             complement(4474..>5125)
                     /locus_tag="PK171_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT05166.1"
                     /translation="GNIPTRGFVPNSMDYFIDTVLLTYDLEKANSLLEEAGYIDSDKN
                     GIREDLAGKDIDLTMLVEPDYVRRSELIKYYLEEVGIKVSMKNVDESTWIALKDQYEY
                     DITVTRSSPWGMMVHGSWGTAYFDSRRTGEGVLHIVDDPIFLGLVDGLLSTTDPEKIK
                     ELAHLVQSYYAETLPAIALYWNMIITPFNREFTGWQPDPLFGIYNIDNFVNLKKVL"
BASE COUNT         1724 a         1070 c          895 g         1436 t
ORIGIN      
        1 cctgtgacat gaaagtctat cctacaagct caatgcttct tcgagagaat taatcttaaa
       61 atcagcaaaa tcgcattcta aaccatcatt caaattaatg aaaaccgttt taattccggc
      121 ctctcgaccg gcctgtatgt cgaggattcg atctccaatc gccaaggtag atcccttttt
      181 taactgatat ttttttacca gataattaaa ggcttctgga tttggttttc gttcatatcc
      241 atcattaccg gttataattt cagtaaaaag ttgcctcatt ccataatgat caagaaaagc
      301 ataaagagaa ggtgattccc gatggctgac gataaagttc ctgcctccct gttgaacaat
      361 ctggcaacaa accttcacgg ctcctgagat aggtggttgc tcgtctaaag aataggattg
      421 gcgaatttct aaatatcttt taaagaacaa ctctcccagt aggtagcgat cttgataata
      481 atcaatcgtt tcagctatcg attttttcat aaaatacagc aaagtatcag gtttttcttc
      541 tattcctaat tctctcaaag ctctttggaa ggcttttacc atcaccggat aggtatcaaa
      601 cagggttcca tcaaaatccc aaatatattc agtatatttc attgatttat ccctctggaa
      661 cgatatccag gtttccattt ttaagtattc ctattgtgtg ggacatatat tgaattactt
      721 caggatcatg agaaataaaa agatatccca aaccatggtt tttttgaagt tcacgaagaa
      781 gaaaaagaat ttgtgcttga actgatatat ccaatgctga agtaggttca tctaaaacga
      841 taaactcagg tttcaacaac aaaatccgag ctaaagaaat ccgttgattc tgacctccag
      901 aaagttgagc aggataacgc aataaaaagt cggttgaaag gccaacggtt tttaaaacat
      961 cttctatagc ctctttctgc tgattgaaag gaactttagt aatattgaga acatcgcgca
     1021 aagaagcgtc tatccgcttt ttggggttta atgatccttg agggtcttga aaaagcatct
     1081 gaacattccg tcggaaaaaa agccaatcag gaggtttcat ccgtgaaata tcctttccat
     1141 gaaagaaaat cgtccctgaa aagggtttta ttaaacctgc taccatttta cctaaagttg
     1201 ttttaccggt tcctgaatgt cccataagac caaaggtttt ccccatatag agtttaaccg
     1261 aaatattact cagaacgatt tggtgttgtt tccttggcca accctgggaa aattcataag
     1321 taaccttgtg cagttcaatc atacaaaaaa caccttacca accgttcatt tactgacatt
     1381 agtcccggtt tttctctatg gcattgttct cttgcccatt gacaacgggg atggaactgg
     1441 catccttgaa gatcttcaat catcgaagga gactgcccag gtatgggatg aaacccattt
     1501 tctggaagac ttctaagaag accttgtgaa tagggatgga atggtgattg aaaaaagtct
     1561 aaagtaggga cttgctcaac tatttctcca gcatacataa ccataatcca atctgatatt
     1621 gaccgagcta ccgttaaatc atgggtaatg agcaacatcg atgatgaatg aatttttcga
     1681 gctcgatcaa gttggtcaat tatttgttta atcaatgttc tatccagccc ttgagttggc
     1741 tcatcagcaa tgatgagatt gggatgaaga gccaaggcag aagcaattag tactctttgg
     1801 ttcatgcctc ctgaaaactc aaaaggatac atttttaccg ccgactctgg cttatcaaac
     1861 ttcatgtagg agagaagacg aaccgactcc tgaattgact ggtttcggtc gacaccttga
     1921 tggattcgaa agggttctcc aacctgccat ccaatagaat aaactggatt taaagccaaa
     1981 gatggattct gaaaaataat cgctatctct ttcccgcgaa tcgaagccat agttttttcg
     2041 tcaactagaa gcaaatcacg accttggaaa agtattcttc cattgatttg agcattgcca
     2101 ggaagaagtc ttaaaatcga ttgggctatc accgactttc cgcaaccggt ttcaccgatc
     2161 aaagccagtg tttcctggtg gggaatcgta atcgatacat tttttactgc tttaactctc
     2221 ccttggtgag tggtaaaaac aacattgagt ctttcaacgg ttaaaaggga caatggcgac
     2281 ttcctctccc attccaattt ctgtttcttc aaatgcaaaa cttaaaaata ccattgataa
     2341 aacaaataat gtaatactca tacctggcgg aatgtaccac caccacattc cattcaggaa
     2401 tccacctctt tgaaaagcat gatgaatcat cattccccag ctttttagag agggatctcc
     2461 caagcctaaa aaagacaacg aagcttccga tatcatggca gaagctacgg tgataagaaa
     2521 ttctggaatg atgatcataa caatgttcgg taagatttca taaaaaagaa tatgccaacc
     2581 tgaaaatccc aaacaacgag cactttcaac aaaacccatt gaacgaatct gcatcgtccg
     2641 agagcggata acccgagcgg ttgaggtcca ccacaaaata ctcataacca gaatcatcaa
     2701 tcctacgctg ggacgtaaaa atgctgaaag aacaattatt aagggtattc ttggaatcat
     2761 tagaataaca tcagtaatac ccattaacaa ttcgtcaatg aatcctccat gataaccggc
     2821 aaaaaggcct atcaataccc caattgatac cgaaaacacc ccggttaaca aaccaaccag
     2881 aagtgtaatc cttgatccgt agataaattc agtgaataga tcattcccaa tatcatttgt
     2941 ccctaaaaga tgttttaatc ctagaggcaa aaaaggatca aaacgatcct tgagctgaaa
     3001 aggggcaata aaattggcaa aaatagcaac caataaaaaa aggataaaga ggaatccacc
     3061 tattcgaatg aaatattttt ttcgttgaaa aatcaagtct ttcatcggtt tgctctcaca
     3121 cgaggatcaa tccaattata aatccaatca gtaagataat tggctataat aaccataaaa
     3181 gaaatgatca aaaaggttcc ctgaagaact gggtagtctc gtgatagtat cgattggtaa
     3241 atgagagtcc ccattccatt aagcgaaaaa acgatctcaa taaagagcgc tccggcaaat
     3301 aaaaatccaa aatcaagagc aaaaagggtt attaccggca gtgaagcatt tttaaaaaca
     3361 tgctgaaaca atatactatc ctccaaatga cctttggctc gagcatacca tacatataac
     3421 ttctctttct cttggagaac actccccctc atcaccatat agtttcgcga agcagaaaat
     3481 aaggtcataa ttaaaaccgg caaaaaaagg tgtttcagaa tatcaatcca atttcctgtc
     3541 gtataaaaac cttttagagg gaaaagaccg agtcgaaatc caaaaaaata gagaaataag
     3601 agagaaataa aaaaaggagg agagcagtaa ataaataaaa agaaaaagaa ggaaaaggta
     3661 ttccctttgc ttttcctttt ccatccagca agagaaccag ccaacgctcc tataatagtt
     3721 ccaagaatca acgaaggagc aaccaagata aaagtccatt ttaagcgtga aatgattaga
     3781 gaagaaactc gagctcggaa atgataagaa taccctaaat caaattgaaa taaattcttc
     3841 caataattag catattgaac taatattggc ctgttcagtc ccaattcttc tcgaattttc
     3901 tcaactgctt cttttccagc aataaaatcc tccccaagaa gattaattac tggatcacca
     3961 ggcatagcac ggggtagaaa aaagtttaaa gtaataataa aaaacagggc gaaaacgtaa
     4021 cgtaatattt tttttgcagg gaaaaccata taatctccat tatcatatcg gttaaaaaca
     4081 agttttaaac aaccaattta gcgcaaccaa ccaggatgcc atcctgggtt ttcaccctca
     4141 cccttacctt ctcttgtttt tctttttttc ctctcccctg gtgggagagg attaaggtga
     4201 ggggggagct tggatattca cctcaaacct taattttaaa aacaccaaac ctgggtttca
     4261 atcagaggtt ccaccctcat cctcaccttc tcccatcaag ggagaaggaa ctttgattcg
     4321 tcattgcgag gaacggagtg acgtgacaat ctcttgagct caatcttttt tattcttttc
     4381 cctttggata aagggagata gtttgggatt cgattggttt ccaaaaaatt caaacccctc
     4441 cttccccctt ttttaaaaga tgagattaaa tgactataat actttcttca gattaacaaa
     4501 attatcgatg ttataaatcc caaagagagg atcaggttgc caaccagtaa attcccggtt
     4561 aaagggtgta ataatcatat tccaatagag tgctattgcc ggtaatgttt cagcataata
     4621 actttgaact aagtgggcaa gctcttttat tttttccgga tcagttgtag aaagcaatcc
     4681 atcaaccaac ccaagaaaga ttggatcatc aacaatgtgc aaaacaccct caccagttct
     4741 tcgggaatcg aaataagccg ttccccagct cccgtggacc atcattcccc atggagaact
     4801 acgagttacg gtaatgtcat attcatactg atctttcagg gcaatccagg ttgattcatc
     4861 aacatttttc atggaaactt tgataccaac ctcttccaag taatacttga tcaactctga
     4921 acggcgaaca taatcaggtt caacaagcat agtaaggtca atatctttcc ctgctaagtc
     4981 ttcccgtata ccattcttat cactatcaat ataaccagcc tcctctaaca aactatttgc
     5041 tttttccaaa tcataggtta ataatacggt atcaataaaa taatccatac tgttgggaac
     5101 aaaacctcga gttggaatgt tgcca
//
LOCUS       DAOSOL010000075         5113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_16458_length_5113_cov_3.588573, whole genome shotgun sequence.
ACCESSION   DAOSOL010000075 DAOSOL010000000
VERSION     DAOSOL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16458_length_5113_cov_3.588573"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PK171_02815"
     CDS             <1..615
                     /locus_tag="PK171_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05167.1"
                     /translation="PLFIHKLAESKSDEEAKIIKLLGEVGLDETCANKYPHELSGGQR
                     QRVAIARALAVSPEFIVCDECVSALDVSVQAQIINLLEDLKNKKNLSYLFISHDLSVT
                     KHISDRVGIMYLGKIVEMADSENICSSPKHPYTKALISSVPIPDPNLKREYISLTEEI
                     PSPINIPTGCGFHTRCPFVTEKCKTLEPELKEVEKGWLVACHFV"
     gene            814..3567
                     /locus_tag="PK171_02820"
     CDS             814..3567
                     /locus_tag="PK171_02820"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOT05168.1"
                     /translation="MENKNIRVAQLSCEYTSNPLGINNLKPLFSWSIHHSERNQKQSA
                     YQIIVASSLENAKKNIGDMWDSKEVNTDQSTVKYQGKLLESKKTYYWIVRVWDKDRNM
                     SPYSEISSFEVGLLYPEDWQGLWIGKPGKGSPLFRKEFKIDKEIKRARVYVSGLGYCE
                     IRLNGEKVGDRVLDPGWTDYDEVVLYSTYDVTSLLRKGSNAVGVMLGNGRFCPPDEVV
                     NKNPYKLKKYHNYPIFILEIYIEFDDDSLVSIISDSNWKTKDGPIIFNDIYDGEIYDA
                     RLEEEGWDVALYDDSAWEFAQEVVSPQGKMKSQATFPFIKVDKILPPKELTSPTPGTY
                     IYDFGQNFSGWVRLKVSGPRGTQVKLRYSELLNEDRSLNTNPNQGAEATDIYILKGQG
                     EEIYEPRFTYHGFRYVEVTGFPGIPSLETLEGCFVHLEVKSTGSLFTSCSLVNKIHEN
                     IRWGQLSNLMSIPTDCPQRDERMGWLGDAQLVSEETIYNFQMIGFFKKWLVDIKKSQK
                     EDGSVPDLVPPYWDFYPADPAWGTACISIPWDLYLYYGDREILEENYSMMERWVEFLH
                     SQSKDGILELGKYGDWCPPWHIISVETPISLVSTWFYYRDTLLQSKIAKILGNVEDYD
                     RFLKRAEEIKISFNKKFLGDKNFYGWTEDWYRTYLPSNASIGDKEITSKRLHRYLRSQ
                     TANILALYSEIVPDDKRKTIWRELIDDIVVVHGNHLNTGIIGTKYILDVLTENGEVDL
                     AFKLVTQATYPSWGYMIREGATTLWERWESLAEEGMNSQNHIMLGTVDAWFYKYLVGI
                     QVDPHFPGWRKIIIKPYPVKDLNFASASLETFRGLISSAWRKDINSFFLQVSIPANSQ
                     AEVQFPKMGWKNIVIEEGGENIWQKGTLVSQKEGIDYAKENEDRIIIQIGSGDYQFKL
                     KEE"
     gene            3616..3750
                     /locus_tag="PK171_02825"
     CDS             3616..3750
                     /locus_tag="PK171_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05169.1"
                     /translation="MGKTTFDLLSGYFIRQLTDRFREERRAGSVRIHLYFIRRQHREI
                     "
     gene            complement(3800..4750)
                     /locus_tag="PK171_02830"
     CDS             complement(3800..4750)
                     /locus_tag="PK171_02830"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOT05170.1"
                     /translation="MNQNQQKISLVGVGRVGSMLALTILMKGLAKEMVLVGENNHQAQ
                     GEAYDLIHASSFAHPMKIYAGPIEATSHSDIIVVSASVPMTNMKNRLDLGTGNATLFR
                     NLIPQLSQLSPEAIFIIITNPVDIMTYYTIRLSNFPPSRVMGTGTLIDSGRFRSLLGL
                     YSGIHPEDIHAYILGEHGESEFPVLSLADFGGMGMENSLPVCRKTCPLKNLAEVFEEA
                     KDGGMLVYRHKGYTNHAIALATCTLIEAIFKDSGRILPVSTLIDGYLGVTDMCLSMPV
                     VLGQEGILKIIELNMNEQEQTAFRSSAAVLKKAAKEMGIE"
BASE COUNT         1610 a          867 c         1068 g         1568 t
ORIGIN      
        1 cctcttttta ttcataaatt agccgaaagc aagagcgatg aagaagcaaa gattattaaa
       61 ttactgggag aagtgggttt ggatgaaacc tgtgcaaata aatatcctca tgaactgagc
      121 ggagggcaaa gacagagggt agccattgct cgggctttgg ctgtttctcc ggagtttatt
      181 gtatgcgatg aatgcgtatc agctttggat gtttcagttc aagcccagat tattaattta
      241 ttagaggatt tgaaaaataa aaaaaattta tcttaccttt ttatatctca tgatttaagt
      301 gtaacaaagc atataagcga tagggttggc ataatgtatt taggaaaaat tgtggagatg
      361 gctgattcag aaaatatttg ttcttcacca aaacaccctt ataccaaagc tcttatttct
      421 tcagtgccta ttcccgatcc taatcttaaa cgagagtata tttcgctaac cgaagaaatc
      481 cctagcccaa ttaacattcc taccggctgt ggatttcaca ctcgttgtcc atttgttaca
      541 gaaaagtgta agactcttga accagagctg aaggaagttg aaaaaggttg gttggtagct
      601 tgccactttg tatgagaaaa gaagaattat ttcacattga agataaaata gaagagtcaa
      661 gtttgtaaca aatttctggg aagagtcttc gtttaaaaat tatcacgttt catagaaatg
      721 agaaatataa ttctgtttta gccctcttat tacaaacgat taattatttg atataagaat
      781 tttccttctt ttaaaaaagg agtaagaaat tatatggaga ataagaatat aagagttgct
      841 caactatctt gtgagtacac ttctaatcca ttgggcatta ataaccttaa acctcttttt
      901 tcttggtcaa tccatcattc agaaagaaac caaaaacaat cagcctatca aataattgtt
      961 gcatccagtc tggaaaatgc taagaagaat attggagata tgtgggatag caaagaagta
     1021 aatactgacc aatcaaccgt taaatatcaa ggtaaattat tggagagtaa aaaaacttac
     1081 tattggatag tacgggtttg ggataaggat agaaatatga gcccttatag cgaaatttcc
     1141 tcttttgaag tgggattatt ataccctgaa gattggcaag gactttggat aggaaaaccg
     1201 ggtaaaggta gtcctctttt tcggaaagaa tttaaaatag ataaagaaat taaaagagca
     1261 agagtgtatg tctccggatt gggttactgt gagatacgct taaatggtga gaaagtggga
     1321 gatagggttc tggatcctgg ctggactgat tatgatgagg tagttcttta ttctacttat
     1381 gatgttactt ctttattaag aaaaggaagt aatgcagtag gggtgatgtt ggggaacgga
     1441 cgtttttgcc ctcctgatga agtggttaat aaaaaccctt ataagctgaa aaagtatcat
     1501 aactatccta ttttcatttt agaaatttat atagaatttg atgatgattc tttggttagt
     1561 attatttctg attctaattg gaaaaccaaa gatggcccga ttatatttaa cgacatttat
     1621 gatggagaaa tatatgatgc ccgtttggaa gaagaggggt gggatgtggc cttatatgac
     1681 gactcagcct gggagtttgc tcaggaagtt gtttctcccc aaggcaaaat gaaatctcag
     1741 gctacttttc ccttcattaa agtggataaa atacttccac ctaaggaact tacctctcca
     1801 actcccggga cttatattta cgattttggt caaaacttca gcggttgggt gcgattaaag
     1861 gtttctgggc ctcgaggtac tcaggttaaa ttaagatact cagagcttct aaacgaagac
     1921 aggagtttaa atacaaatcc gaaccaaggc gctgaagcca ccgacattta tattttaaaa
     1981 ggacagggag aagaaattta tgaacctcgc tttacttacc atggttttcg ttatgtagaa
     2041 gttacgggat ttcctggtat cccttcttta gaaacattag aagggtgttt tgtccattta
     2101 gaggttaaat ctacaggaag tctttttacc tcttgttcat tggtgaataa aattcatgaa
     2161 aatattcgct ggggtcaact tagtaattta atgagcattc ccactgattg tcctcaaagg
     2221 gatgagcgaa tgggatggtt gggagacgcc cagctggttt cggaagaaac tatatataat
     2281 tttcaaatga tcggattttt taaaaaatgg ttggttgata ttaagaaatc ccaaaaagaa
     2341 gatggaagcg ttccggattt ggtgcctcct tattgggatt tttatccagc agatccagct
     2401 tggggtactg cttgtataag cataccttgg gatttgtacc tctattatgg tgatcgagaa
     2461 attttggaag aaaactattc tatgatggag agatgggtag aatttctcca ctctcagtca
     2521 aaagatggta tcttagaatt gggaaagtat ggtgattggt gtcctccttg gcatattata
     2581 tcggtagaaa ctccgatttc tcttgtttcc acttggttct actatcgcga cactctcctt
     2641 caatctaaaa tagcaaaaat tttaggaaat gttgaagatt atgacagatt cctcaaaaga
     2701 gccgaagaaa taaaaatatc cttcaataaa aaattcttag gagataagaa tttctatggt
     2761 tggaccgagg attggtatcg tacttattta ccttcaaatg cttctatagg ggacaaagaa
     2821 atcacatcga aaagactaca taggtatctt agaagccaaa cagctaatat tcttgctctt
     2881 tattcagaaa tagtacctga tgataaaagg aaaactatat ggagagaatt aatagatgac
     2941 attgtagtcg ttcatggtaa ccaccttaac accgggatta ttggaacaaa atacatctta
     3001 gatgttttaa ctgaaaatgg agaagttgat ttagctttca agttagttac tcaggccact
     3061 tatcccagtt ggggatatat gataagagaa ggagcaacca ctctttggga gcgatgggag
     3121 agtcttgccg aagaaggtat gaactctcaa aatcatatta tgctaggaac ggttgatgcc
     3181 tggttctata aatatttagt aggtattcag gtagatcctc attttccagg ctggagaaaa
     3241 attattatta aaccttatcc agtcaaagat ctaaattttg ccagtgcttc cctggaaact
     3301 tttcgaggtc ttatttcctc cgcttggagg aaagatatta attctttctt cctacaggta
     3361 tcaataccag caaatagcca agccgaggtt cagtttccta agatgggttg gaaaaatata
     3421 gttattgaag aaggaggaga gaatatttgg caaaagggaa ccctggtttc tcaaaaagag
     3481 ggaattgatt atgctaagga aaatgaagat agaataatta tccagattgg atcaggggat
     3541 tatcaattta aattaaaaga agagtaaagc aataagttgt taactcgctg aagggttttt
     3601 aataaatatc attttatggg aaaaactact ttcgatttac tatcaggtta ttttatccga
     3661 cagctcaccg atcggtttcg agaagaacga cgagctggtt cagtccgtat tcatctctat
     3721 ttcatacgcc gacagcatcg agagatttaa agagtgatgt tcctattgtt cttttagtat
     3781 tcttcccatt gctatacttt tattcaattc ccatttcttt tgcggctttt ttaagtacgg
     3841 ctgccgaaga gcgaaaggca gtttgttctt gttcgttcat atttaattcg atgattttta
     3901 aaattccttc ttgacccaaa acaaccggca ttgataaaca catgtcagtt actcctaaat
     3961 agccatcaat caaggtgcta accgggagga ttcgacctga atctttaaat atggcttcga
     4021 tcaacgtaca ggttgccagg gcgatagcat gattggtgta tcctttatgt cgataaacta
     4081 acattcctcc atctttagct tcttcaaaaa cttcggcaag gtttttcagt ggacaggttt
     4141 tccgacagac aggcaaagaa ttctccattc ccattccacc aaaatcagcc aggctcagaa
     4201 ccgggaattc actttcaccg tgctcaccta aaatgtaagc atgaatgtcc tcggggtgga
     4261 tgccgctata tagcccaagc aaggaacgga agcgcccact atcgatcaaa gtgccggttc
     4321 ccatgactcg ggaaggagga aaattcgata aacgtatggt gtaataggtc atgatgtcca
     4381 ctggattagt tataattatg aaaattgctt cagggctgag ttgggaaagt tgaggaatga
     4441 gattccgaaa aagagtagca ttaccagtac ccaaatctaa acggtttttc atgttggtca
     4501 taggaactga tgctgaaacg acgatgatat ccgagtggct ggttgcttca atcggtccgg
     4561 catagatttt catgggatgg gcaaaagaac tggcgtgaat aaggtcataa gcttcacctt
     4621 gagcttgatg gttgttttca ccaactaaaa ccatttcttt ggctaaaccc ttcatgagaa
     4681 tggttaatgc cagcattgat ccgactcttc caactcctac cagtgatatt ttttgttggt
     4741 tttgattcat gagacattct cctatcctta ggaatggtga tgaatagtca ttttatcttc
     4801 atcctgacct tttcccatca atggaagatc aagaaattat attgattcat catcctgagt
     4861 ccttactttt cgagggtgcg aggacctcat tttttaatta tattataaaa gatatttttc
     4921 tataagagat tgccacgtcg ttcgaccaaa aggaaggttg aactcttcac aatggcggat
     4981 tgagcagttg agatcctcac gcggaaaaac accaatctgg atgacagatt aaagacaccc
     5041 tccccgccgc aaagcgacac ccctccaagg aggggaattt ttaaatatat tcccccatta
     5101 aggggggatt aag
//
LOCUS       DAOSOL010000076         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_17147_length_4951_cov_3.522672, whole genome shotgun sequence.
ACCESSION   DAOSOL010000076 DAOSOL010000000
VERSION     DAOSOL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17147_length_4951_cov_3.522672"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PK171_02835"
     CDS             complement(<1..483)
                     /locus_tag="PK171_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT05171.1"
                     /translation="MKLHLMTGHDFTHAIQKRWPLFIPAGTIEYHGEHLPLGVDTLAV
                     IKALDEVEKKINCVVAPPVWFGPSSFAVAGPEMGTINVDTNQFEKQANDILHGFLENG
                     FRQIFVVIHHQYEMGQMMPEALAFKKASVSLIFRLLEKEHGKGWWGSEKMSNYYNNLQ
                     T"
     gene            complement(480..1655)
                     /locus_tag="PK171_02840"
     CDS             complement(480..1655)
                     /locus_tag="PK171_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05172.1"
                     /translation="MGQSNKENFIQIFRGEPADRILWIADLTYWRDSQIIKGILPDEY
                     RGIDGFLKLHKDLGVVPYYIYALDEQEPDGMSVHQIGAPGRPFNGVFQYSFEGVDIET
                     IVNGTILETKFHYQEKTFIQKKAYLPQSFCYAFIQYPIQKAEDLKCLRKIYEQCIFRS
                     NYSDYLEIGEKWGDEGIPIAPLPRSPLSALIVDWMGIEAFTYAQVDEPIEIQKTIDCI
                     DESNDQAFQIIIKSPAEIFHFCDNLSGTSYSSFFPRYAANYYARRFIQLHQAGKKAAV
                     HIDGTLKGIINQIAATGADALEALTPDPVGDVTVEDLRKESQSENIILWGGLPAVMFT
                     PFFAREDLIKQVNRIQKQWKENPRFIAGSADQIPPDADLDYLRLASELFNRGRRIDS"
     gene            complement(1639..2463)
                     /locus_tag="PK171_02845"
     CDS             complement(1639..2463)
                     /locus_tag="PK171_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05173.1"
                     /translation="MKGSKLKARDRWIDVLVFLVVILVLFPIVWTFLTSIKGRFDISA
                     MPPKLLFTPTIENYRKVFIEDGFYRYFKDSIIIALIATVIAIFLGTMTAYGLVRYRVG
                     GKFLPFWILSTRMLPPIAIILPIYILMQRIKLIDTYASIIIMHVLLNLPFAVWLMSGF
                     FREVPSEIDEAAMLDGCSVFGAFIRVLFPVVIPGVLATTVFCLITSWNEFLFALILSG
                     RNVKTLPVAAAFYVTDRDILWGPMAAVAVIASIPIIIFTFLIQKHLVRGLSYGAIK"
     gene            complement(2460..3332)
                     /locus_tag="PK171_02850"
     CDS             complement(2460..3332)
                     /locus_tag="PK171_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05174.1"
                     /translation="MSSIKNRRFNIPQLMVLPTIIFILAVSVYPLIYSFYMTLHDWSL
                     TRAEPPTFVGIGNYISLFQDSRFWYSFRTTVIFTGWAVVMEMVIGMTLALSITRNTRF
                     QQICRSILLIPMMITPVVLSLMWKYMYNPEYGIFNYFLYFLKIEGPIWLGEPSPALPA
                     VIMVDIWQWTPFVFLLLFSGIASLPPDVFEAAQVDGATGFQTFRNITLPLVTPFLLVA
                     LLIRFMDSFRIFDTIYVLTKGGPANATETLSIYTYKVGFNYFNMGYAATLSYIILVII
                     TIASQQFVKLEGKS"
     gene            complement(3416..4747)
                     /locus_tag="PK171_02855"
     CDS             complement(3416..4747)
                     /locus_tag="PK171_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05175.1"
                     /translation="MKRSTKKCIIFGLVLSLIFIFALVAQGADKPVLRTLVFQGWART
                     PSLQKQVLEYEKLTGVKVEIEEVPFSQFHEKMILDFASKAGTYDFIGTLTDWFSELVR
                     GGYIIPIDDYIKNDPPEDWPNTYPQALLNLQTKDGKLYGFPTHDGPIMLYYRKDLFED
                     PKEKEAFKEKYGYELQSPKTWAQFLDMAIFFNRPDQNLNGTVIAAKQGGQQLAYDFYL
                     MLWSFGGDIFDENWKPIFNSQAGIDGLQYYADLRNKWGVTPKASTTYDETESGPIYLE
                     GRAALMWHWSHIASWAEMEDRSKIIGKNGYTVFPVADPNIPHTTLDIHWFFAISSASK
                     NQDETYKLVKWLCNKENDKEAAFMGTVACRLSTFHDPDVLEKFPFYSGIEAALAGEAK
                     TTPQIAEYAQVDDLIGIACSKVIAGEKTAKEALDEAAKQVEDLMAKAGYYK"
BASE COUNT         1595 a         1020 c          871 g         1465 t
ORIGIN      
        1 cgtttgcaga ttattatagt aatttgacat tttctctgat ccccaccatc cttttccatg
       61 ttccttttct aatagtcgaa aaataagtga aactgatgcc tttttaaaag ctaaagcctc
      121 gggcatcatt tgccccattt catattgatg gtggatgact acaaaaattt ggcgaaagcc
      181 attctctaaa aaaccatgga ggatatcatt tgcttgtttt tcaaactgat tcgtatctac
      241 attaatagtt cccatttctg gaccagctac tgcaaaactg gaaggtccaa accaaactgg
      301 tggggctaca acacaattaa tctttttttc aacctcatca agagctttga tcactgctaa
      361 ggtatccaca cccagcggta aatgttcacc atggtattca attgtccctg ctgggataaa
      421 aagaggccaa cgtttttgaa tagcatgagt aaagtcatga ccagtcatca gatgtaactt
      481 catgagtcaa ttctcctccc acgattaaat aattctgatg ccaaacgaag gtaatccaaa
      541 tcagcatcgg gtggaatttg atctgccgat ccagcaataa accttggatt ttccttccat
      601 tgtttttgaa ttctattgac ttgttttatt aaatcttccc ttgcaaaaaa aggagtaaac
      661 ataacagctg gaagacctcc ccataaaata atattttcac tttgactctc ttttcttaaa
      721 tcctcaactg taacatctcc taccggatcc ggagtgagag cttctaaagc atcagccccg
      781 gttgctgcaa tttgattaat gatcccttta agggttccat cgatatgaac tgctgctttt
      841 ttccctgctt gatgaagttg aataaagcgt cgagcataat agttggctgc ataacgtggg
      901 aaaaaagaag aataacttgt tccactcaga ttatcacaaa aatgaaaaat ttctgcggga
      961 gattttatta taatttgaaa ggcttgatca ttcgattcat caatacaatc aatggttttt
     1021 tgaatttcta ttggttcatc cacttgagca taagtaaaag cttcgatacc catccagtct
     1081 acaatcaatg ctgagagtgg acttcttggc aggggagcaa tcggtattcc ttcatctccc
     1141 catttttctc caatttcgag ataatctgaa taattagatc tgaaaataca ctgctcataa
     1201 attttccgaa ggcatttcag gtcttctgcc ttttgaatgg ggtattgaat gaatgcataa
     1261 cagaaggact gaggaagata agcctttttc tgaataaatg ttttttcttg ataatgaaac
     1321 tttgtttcaa gaatagttcc attaacgata gtttcgatgt caactccttc aaaagaatat
     1381 tgaaagactc cattaaaagg tctccctggt gcaccaatct gatgtactga cattccatcc
     1441 ggttcctgtt catctaaagc ataaatataa taaggaacta ctcctaaatc tttatgtagt
     1501 tttaaaaaac catcaattcc tcgatattca tcgggtaaaa ttcccttgat tatctggctg
     1561 tcccgccagt aggttagatc agctatccac aaaattcgat ccgccggctc tcctcgaaat
     1621 atttggataa aattctcttt atttgattgc cccataggag agccccctta ccaaatgctt
     1681 ttgaattaaa aaagtgaaaa taataattgg gatagaagca ataactgcta ccgctgccat
     1741 tggtccccag aggatgtccc ggtcggtcac ataaaatgcg gcagcaactg gaagggtttt
     1801 gacattcctt ccagaaagaa ttaaagcaaa taagaattcg ttccaggacg tgattaaaca
     1861 aaaaaccgtg gttgccaata ccccaggaat cactaccgga aaaagaacac gaatgaatgc
     1921 cccaaaaacc gagcatccgt cgagcattgc tgcttcatcg atctcagaag gtacttcccg
     1981 gaaaaaacca ctcataagcc acactgcaaa agggaggttc aataaaacat gcataataat
     2041 aattgaagca taagtatcaa tcagctttat cctttgcata aggatataaa tcggtaaaat
     2101 gatagcaatt ggtggaagca tacgagtcga taaaatccag aaaggaagaa acttacctcc
     2161 tactcgatat cgaaccaatc cgtaagcagt catggttcct aaaaaaattg caatgacagt
     2221 tgcaattaaa gcaattatta tagagtcttt aaagtatcga taaaaaccgt cctcaatgaa
     2281 gactttccga taattttcaa tggtgggtgt aaacaacaat ttaggtggca tagctgaaat
     2341 atcaaagcgt cctttaattg aagttaaaaa ggtccataca ataggaaata aaaccaagat
     2401 aaccactaaa aaaactaaaa catcgatcca tcggtcccga gctttaagtt tggaaccttt
     2461 catgattttc cctccaattt tacaaactgc tggctcgcaa tggttatgat tactaaaata
     2521 atataagaga gagttgccgc ataccccata ttgaagtaat tgaatcctac cttataagta
     2581 taaatactca aggtttcagt ggcatttgca ggacctcctt tggtcaagac ataaatagtg
     2641 tcgaaaatcc gaaatgaatc cataaagcga attaaaagtg cgaccaataa aaagggggtg
     2701 accagaggaa gagtaatatt acggaaagtt tggaaaccag tggctccatc tacctgggct
     2761 gcttcaaaaa cgtccggagg gagactggca attccagaaa acaaaagaag aaaaacaaaa
     2821 ggcgtccatt gccagatgtc aaccataatg actgccggaa gcgccggaga cggctctcca
     2881 agccaaatgg gtccttcaat tttcaaaaaa taaagaaaat aattaaaaat cccgtattct
     2941 gggttataca tatatttcca cataagagat aaaacaactg gagttatcat cattggaatc
     3001 agtaaaattg accggcatat ttgttgaaaa cgggtattgc gagtaataga aagggctaaa
     3061 gtcattccga ttaccatttc catcactaca gcccatccag taaaaataac tgtggttcga
     3121 aaagagtacc aaaaacggct atcttgaaat aatgatatat agttccctat acctacaaaa
     3181 gttggtggtt cagcccgggt caggctccaa tcatgaagtg tcatatagaa agaataaatt
     3241 aatggataaa cactcacagc aagaataaaa atgatagtag gaagaaccat taattgaggt
     3301 atattaaacc ttctattttt aatggatgac ataatatccc tccgtggatg ctaccggaac
     3361 aaaatttccg gtagcatcca accccatatt caaatcaaat cttaaataaa catatttatt
     3421 tataatatcc agcttttgcc attaagtctt ctacctgttt agccgcttcg tctaatgctt
     3481 ccttagcagt tttttctccg gcaattactt ttgagcaagc aatgccgatc aagtcatcaa
     3541 cctgagcata ttcagcaatt tggggggttg ttttcgcttc accagctaaa gctgcttcta
     3601 taccgctata gaagggaaat ttttctaata catctggatc gtggaaagtg gataatcggc
     3661 aagcaacggt acccataaaa gcagcttcct tatcgttttc tttgttacaa agccatttta
     3721 cgagcttata agtttcatct tggtttttac tagcgcttga tatagcaaaa aaccagtgaa
     3781 tgtctaaagt tgtatgtggt atgttgggat cggctactgg gaatactgta tagccatttt
     3841 ttccaataat ttttgatcga tcctccattt ccgcccaaga agcaatgtgg ctccaatgcc
     3901 acatcaaagc tgctcttcct tcaagataaa tcggaccact ttcagtttcg tcataagtag
     3961 tcgatgcctt gggcgttact ccccatttgt ttctgaggtc agcatagtac tgtaaaccgt
     4021 caatccctgc ctgagaatta aagattggtt tccaattttc atcaaaaata tctccaccaa
     4081 aagaccaaag catcagataa aaatcatagg ccaattgctg acctccctgc tttgcggcaa
     4141 tcacggttcc gttcaaattt tgatcgggtc ggttaaaaaa tattgccata tccagaaact
     4201 gagcccaggt ttttggggat tgtaactcat aaccatattt ctctttaaac gcttcttttt
     4261 ccttgggatc ttcaaacaaa tccttccgat aataaagcat aataggccca tcatgagtag
     4321 gaaacccata gagtttgcca tctttagttt gcaggttgag aagtgcttga ggatatgtgt
     4381 taggccaatc ttcgggagga tcatttttga tgtaatcatc aatgggaatt atataaccac
     4441 cgcggactaa ctcagaaaac caatctgtca gggttcctat aaaatcatag gttccggctt
     4501 tgctggcaaa atcgagaatc attttttcat gaaattgaga aaaaggtacc tcctcgattt
     4561 ctacttttac tccggttagt ttttcatact ctaaaacttg cttttgcaag gatggagttc
     4621 ttgcccatcc ctggaaaacc aaggttcgta aaactggctt atctgcaccc tgagctacca
     4681 gtgcaaatat gaatattaaa cttaaaacca aaccaaaaat gatacacttt tttgttgatc
     4741 ttttcattct atgcccctcc ctttaagggt tttaataaaa tacttaaatt gaattactca
     4801 tcaggattct ttcattcctg attcctattt tattcctctt ataccacctc catttttatc
     4861 ctgaaaatct atatgcgggc atgataaatc aagcccctac caaagaatta aaaacctagg
     4921 ggcacaatgt attatgcccg ttcttcattt a
//
LOCUS       DAOSOL010000077         4947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_17166_length_4947_cov_4.177228, whole genome shotgun sequence.
ACCESSION   DAOSOL010000077 DAOSOL010000000
VERSION     DAOSOL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17166_length_4947_cov_4.177228"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..333)
                     /gene="rpmC"
                     /locus_tag="PK171_02860"
     CDS             complement(112..333)
                     /gene="rpmC"
                     /locus_tag="PK171_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOT05176.1"
                     /translation="MKASEIRNMTGDELNKKLSDLRTELFNLRFQAITGQLGNPRRIH
                     MVKKDIARIKTIVNEREMFGKTQEVKSNG"
     gene            complement(296..755)
                     /gene="rplP"
                     /locus_tag="PK171_02865"
                     /pseudo
     CDS             complement(296..755)
                     /gene="rplP"
                     /locus_tag="PK171_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
     gene            complement(733..1449)
                     /gene="rpsC"
                     /locus_tag="PK171_02870"
     CDS             complement(733..1449)
                     /gene="rpsC"
                     /locus_tag="PK171_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT05177.1"
                     /translation="MGQKVNPIGLRLGIIRGWQSRWYADKKYREYLAEDLRIRQFVRE
                     KLQRAGVSAVEVERLANQVKVIIKTSRPGIVIGRKGSEVESLRQSIQKLVDNPNVQIT
                     VDEIKVPEIDAYLVAENVAFQIERRVSYRRAMKQAITRALRSGAIGIKISCSGRLSGA
                     EIAREEWYREGRLPLQTLRADIDFGFTEANTTYGKIGVKVWIFKGEVIKTGEVLEGVS
                     STKVDNRKEETEAYVDSEES"
     gene            complement(1450..1794)
                     /gene="rplV"
                     /locus_tag="PK171_02875"
     CDS             complement(1450..1794)
                     /gene="rplV"
                     /locus_tag="PK171_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT05178.1"
                     /translation="MEARATAKYIKISPRKARQVVDIIRGKKADEALSLLQFVPKKAS
                     RLVYKVLKSAVANAENNLNLNTDSLVVSKAFVNQGPTMKRIRARAMGRANVIRKRTSH
                     ITIAVSDMEEGS"
     gene            complement(1812..2096)
                     /gene="rpsS"
                     /locus_tag="PK171_02880"
     CDS             complement(1812..2096)
                     /gene="rpsS"
                     /locus_tag="PK171_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT05179.1"
                     /translation="MVRSSKKGPYVDLKLRSRIEEMNKKGEKKVLKTWSRASMIIPDM
                     IGHTIAVHNGKKHIPVYITEQMVGHKLGEFAPTRTFKGHGNPTERSTALR"
     gene            complement(2115..2939)
                     /gene="rplB"
                     /locus_tag="PK171_02885"
     CDS             complement(2115..2939)
                     /gene="rplB"
                     /locus_tag="PK171_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT05180.1"
                     /translation="MSNIKAYKPTTNPRRHMTGHTFEEVTPGGPEKSLLVSHRRKAGR
                     DSSGRITVRHQGGGHKRKYRIIDFKRDKFDIPAKVSRIEYDPNRSARIALLVYADGEK
                     RYIIAPLGLKVGDTVLSSPKAEIQPGNALPLRSIPVGTIIHNIEMKQGKGAQLARAAG
                     SYGQLMAKEGTYTQIRLSSGEVRLIHIDCLATVGQVGNLDHENITVGKAGRKRWMGIR
                     PTVRGNAMNPIDHPHGGGENKAHCGRQPVTPWGQYTKGYKTRKNKNTDKWIVKRRK"
     gene            complement(2960..3247)
                     /gene="rplW"
                     /locus_tag="PK171_02890"
     CDS             complement(2960..3247)
                     /gene="rplW"
                     /locus_tag="PK171_02890"
                     /inference="COORDINATES: protein motif:HMM:NF004366.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT05181.1"
                     /translation="MTQDREILIHPIVTEKAVDQQKTNKYVFRVDPRATKNDIKNAVH
                     NLFGVTVLQVNTMNVKGKVKRMGRFEGYTRSWKKAIVYVKPGEKIKAFDIG"
     gene            complement(3244..3867)
                     /gene="rplD"
                     /locus_tag="PK171_02895"
     CDS             complement(3244..3867)
                     /gene="rplD"
                     /locus_tag="PK171_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOT05182.1"
                     /translation="MELEVKNREGNKVGTLNIEDTLFQREINTHLIFLSVKGYLDNQR
                     QGNAKTKTRSEVRGGGKKPWRQKGTGRARHGSNRSPIWRHGGIVFGPQPRSFSHPLPK
                     NEKRLALINAITDKLNNNAVMVIDQLNIDRPKTKDAVELLNRLNVQGNVLIVVSEKNP
                     NVLRAFANLPLVKLLSRNELNTYSIVKSDMVVFEKDVLVGIQEVYGA"
     gene            complement(3878..4510)
                     /gene="rplC"
                     /locus_tag="PK171_02900"
     CDS             complement(3878..4510)
                     /gene="rplC"
                     /locus_tag="PK171_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT05183.1"
                     /translation="MPLIEYGMIGEKVGMSRIFSDKGESIPVTVIKCGPCYIVQKKEK
                     DTDNYEAVQIGYGETKEKKVNKPIRGHLKKAGIEPLRKLVEFRVKDLSAFQVGQVLKV
                     SGFKVGDTIDIVGKSKGKGFAGVMKRHGYRGGKASHGSMHRRAPGSIGGTDAGRVWKG
                     KGLPGRMGGERVTMKNLTVVQINDERDLLVVKGAIPGAPGQTVLVRKNAV"
     gene            complement(4541..4846)
                     /gene="rpsJ"
                     /locus_tag="PK171_02905"
     CDS             complement(4541..4846)
                     /gene="rpsJ"
                     /locus_tag="PK171_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT05184.1"
                     /translation="MAQKIRIKLKAYDHKILDQSALRIVQTAEKTGAFVSGPVPLPTD
                     IVKYTVLRSSHVDKKSREQFEQRTHKRLIDILEPNPKTVDALMHLDLPAGVDIEIKL"
BASE COUNT         1235 a         1221 c          846 g         1645 t
ORIGIN      
        1 gattttttca tttttttctt ataaaaagga tgttcgctaa tccgtattac tcgacccagt
       61 acagttttat ccattttatt actgagaatt tcacctgtga atctttttcg gtcagccatt
      121 tgatttgacc tcctgcgtct ttccaaacat ttcacgttca ttgacaatgg ttttgatccg
      181 ggcaatatct tttttaacca tgtgaatcct tcgaggattc cccaattgtc ctgttatcgc
      241 ttgaaatcgt aaattaaaaa gctcggttcg caggtcactc aactttttat taagctcatc
      301 cccagtcatg ttgcggattt ccgaagcttt cattaacaac acctctattc tgcttttctc
      361 tcaactattc ttgttttaat gggcaacttc tgagcagcca ggtgaagtgc ctcataggca
      421 atttcccggt caacacctga tacttcaaaa agaacgcgtc cgggtttgac caccgctacc
      481 catccctcaa ctggtccctt accttttcct tgacgagttt cagtcggttt ttttgtaaaa
      541 ggtttatctg gaaaaatccg aatccagatc tttccctttt taagatgccg agaaatggcg
      601 attcgagctg cttcgatttg aggattcgtt atccattgtg gttctaatgc ttgtaaaccg
      661 aattcgccaa aatctacggt tgcaccgcgg gtttcaacac cactcatcct tccacggttt
      721 tgttttctat atttaactct cttcggaatc aacatatgct tcagtctcct ccttacgatt
      781 atcaaccttg gtactggata ctccttccag aacctcaccg gttttaatca cttcaccctt
      841 aaaaatccac actttcactc cgattttgcc ataggtggtg ttcgcttcgg taaatccaaa
      901 atctatatca gcccgaagag tttgcaaggg taatcgaccc tcacgatacc attcttctcg
      961 tgcaatttct gctccggaga gtcttcctga acaggaaatt tttattccaa tagcacctga
     1021 tcgaagagca cgagtaatgg cttgtttcat tgctcgtcga taggaaaccc ttctttcaat
     1081 ttgaaaagca acattttcag caaccaagta agcatcaatt tctggaacct taatctcatc
     1141 aacagtaatt tgaacatttg gattatcaac caatttttga atcgattgac gaagactttc
     1201 aacttcagat ccttttcgtc caattacaat tcctggccgt gaagttttta taattacctt
     1261 aacctggttg gcaagtcttt ctacttcaac tgctgaaaca ccggctcttt gcaacttctc
     1321 tctaacaaac tgtcgaattc gaagatcttc tgctaaatat tctcgatatt ttttatctgc
     1381 ataccagcga gattgccatc ctcgaataat tcctaaccta agtcctatcg gattaacttt
     1441 ctgtcccaat tagcttccct cctccatatc tgatactgca atggtaatat gagaagtcct
     1501 ttttcgaata acatttgctc ttcccattgc tcgagctcga attcttttca tggttggacc
     1561 ttgatttaca aaggcctttg aaaccaccaa actatcggta tttaagttaa ggttgttttc
     1621 tgcattggca actgccgact taagcacttt ataaaccagt cgggaagctt ttttgggaac
     1681 aaattgaagc aaagataaag cttcatcggc ttttttccca cggatgatat caacaacttg
     1741 tcgagctttc cgtggtgata ttttgatata ttttgcggtc gctctcgctt ccatctttgt
     1801 tatcctccct cttatctgag cgccgttgat cgctcggtag gatttccgtg gcctttaaag
     1861 gttcgggttg gcgcaaactc gcccaattta tgccctacca tttgctcagt gatataaacc
     1921 ggaatatgct tttttccatt gtgaactgca atagtatgcc cgatcatgtc cggtataatc
     1981 atactggcac gcgaccaagt tttcagtact ttcttttcac cttttttatt catctcttca
     2041 atacgcgaac gtaattttaa atcaacatat ggaccctttt tcgatgatcg taccaaaagg
     2101 aattcctcct cccattattt tcttctcttc acaatccatt tatcggtatt tttattcttg
     2161 cgggttttat accctttcgt atactgtccc caaggcgtta ctggttgccg cccacaatga
     2221 gctttatttt ctccacctcc gtggggatgg tcaatcgggt tcatagcatt accacgaact
     2281 gtgggtcgaa tacccatcca acgttttcga ccagctttcc caaccgtgat gttttcgtga
     2341 tcaagattcc ccacttggcc tacggtggcg agacaatcaa tatgaataag ccgaacttca
     2401 ccagaagata accgaatctg agtataggtt ccttcttttg ccataagttg cccataggag
     2461 ccagcagctc tggctaattg agctcccttt ccctgtttca tttcaatgtt atgaataata
     2521 gttccaaccg gtatactcct taacgggaga gcattgccag gttgaatttc cgcttttggg
     2581 ctggataaca cggtatcacc aactttcaaa cccaaaggag caattatgta tcgtttctct
     2641 ccatccgcat agaccaagag agctatccgt gctgatcggt tgggatcata ttcaattctg
     2701 gaaactttag ctgggatatc gaatttatcc cgtttaaaat cgatgatacg gtatttccgt
     2761 ttgtgaccac caccctgatg acgcaccgta attcttcccg aactatctct tccggcttta
     2821 cgccgatgag aaacaagaag cgatttttct ggtccccctg gagtaacttc ctcgaaagta
     2881 tgcccggtca tgtggcgtcg ggggttcgtt gtcggcttgt atgccttaat atttgacata
     2941 gtaataatct cctttagaat tatccgatat caaaggcttt tattttctcg ccaggtttga
     3001 cataaacaat cgcttttttc catgaacgag tatatccctc aaaacgtccc atacgtttca
     3061 cttttccctt tacattcatg gtgttgacct gtaatactgt aactccaaac aaattatgaa
     3121 cggcattctt gatgtcattt ttggttgccc ggggatcaac ccgaaacaca tacttattgg
     3181 ttttttgctg gtcaacagct ttttcagtaa caatcggatg aatcaatatc tctcgatctt
     3241 gagtcatgcc ccgtacacct cctgaattcc aaccaaaaca tccttttcaa aaaccaccat
     3301 atcactttta acaatactat aagtattgag ctcattgcga cttaacaatt tcaccaaagg
     3361 caaattcgca aaagcccgga ggacattagg gtttttctct gaaacgacta ttaaaacatt
     3421 accttgaaca ttcaaccgat taagaagttc aacagcatct ttggttttcg gtctatcaat
     3481 attgagctga tcaataacca tgacagcatt attattcaat ttatctgtta tggcattaat
     3541 taaagccaac ctcttttcat tttttggtag aggatgacta aacgatctgg gttggggtcc
     3601 aaacacaatt cctccatgac gccaaatcgg agaccggtta cttccgtgcc gggctcgtcc
     3661 ggtacctttt tgccgccagg gttttttccc accaccacga acctcgctac gggttttggt
     3721 tttagcgttt ccctgccgtt ggttatcaag atatccctta accgagagaa aaatgagatg
     3781 ggtgtttatt tctctttgaa agagagtatc ttctatatta agggtaccaa ccttgttccc
     3841 ttctctattc tttacctcaa gttccatttc tttcctccta aacggcattc ttccgcacaa
     3901 gtacggtttg accgggtgca ccaggtattg cacctttcac cactaataaa tctcgttcat
     3961 cattgatttg caccacggtt aaatttttca tcgtcactct ctctccaccc attcttccgg
     4021 ggagaccttt ccctttccaa actctcccgg catcggttcc tccaattgaa cctggagctc
     4081 ggcgatgcat agagccatga gaggctttcc ctccccgata accatggcgt ttcattactc
     4141 cagcaaatcc ttttcccttc gatttcccaa cgatatcgat ggtgtctcca actttaaatc
     4201 ctgatacttt aagaacctgg ccaacttgaa acgccgacaa atcttttacc cgaaattcaa
     4261 ccaattttct tagaggttca attccagcct tctttaggtg acctctgatt ggtttgttta
     4321 ctttcttttc cttggtttct ccatatccga tttgtactgc ttcgtaatta tcagtatcct
     4381 tttccttttt ttggacgata taacaggggc cacatttaat taccgtaacc ggtatagatt
     4441 cgcccttatc ggaaaagatc cggctcatac ctactttttc tccaatcatg ccatactcta
     4501 ttagtggcat tctatttctc tccttccgtt tccaccgttt ctacagtttg atttcaatgt
     4561 caaccccagc cggtaaatca agatgcatca atgcatcaac ggtctttgga ttcggttcta
     4621 atatatcaat cagcctttta tgggttcttt gttcaaattg ttctcgagat tttttatcta
     4681 catgagaaga gcgcaaaacc gtatatttaa caatatcagt cggcaacgga accgggccag
     4741 aaacgaatgc tccagtcttt tccgctgtct gaactatccg taaagctgat tgatctaata
     4801 ttttatggtc ataggctttc aattttattc ttattttttg tgccactttc atgcctccca
     4861 gtaaaggaat ttcccaaata caggcaaatg aattacccga taatatcggt tactactccg
     4921 gctccgacgg tacgaccacc ttcacgg
//
LOCUS       DAOSOL010000078         4861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_17524_length_4861_cov_3.715772, whole genome shotgun sequence.
ACCESSION   DAOSOL010000078 DAOSOL010000000
VERSION     DAOSOL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17524_length_4861_cov_3.715772"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1032)
                     /locus_tag="PK171_02910"
     CDS             complement(49..1032)
                     /locus_tag="PK171_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05185.1"
                     /translation="MKPGSEQFRSILILTLINVGIIVILSFLSPTFLTYENLLSVLKR
                     MSELGMLAIAETIVFISGGFDLSIGTIMAISGLIAGQMYIFGLPFFLCVLLALLGGMA
                     AGLMNALFIVKLRLQPFIVTLATMSILRSIVYGVLRGKTLSHFPESYLAYGDLTILGF
                     PFLFLVLIVFAVLSSLLLQRTYIGRQIYAVGGNERSAYLSGISVNRIKYTVYILSGFI
                     CACAGLLFSIRIRAIIPDAGLNSPLEVVTAVVIGGTAITGGKGTIAGSLLGVLALFLL
                     TNGFSLLNLNPFWQLVVLGLLLIYVVGREGISKALIGKRQKKKPHQTVAIK"
     gene            complement(1029..1997)
                     /locus_tag="PK171_02915"
     CDS             complement(1029..1997)
                     /locus_tag="PK171_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05186.1"
                     /translation="MNMPLEQKIQEKRRPLRNFRELPIFLYILGMLIVFSFVIPGFFS
                     ARNFINISRQIAVVGILSMGMTLAILTAGIDLSVGSILSFAISIGGMGIPDGRTIWVV
                     YPLVLVLGLILGLVNGLLVTRIAVPAMIITLGTMNIYRGITMIITQGKYITPIPSQFL
                     YIGRGLTPFLFLLGTVILFIYITLFTRFGRNIYAIGGSEQSAIYSGVPVQKYKIAVYA
                     ISGILSAFAGLIFVGRSGFIQPQAGIGYEMNAIAAVVIGGTSITGGAGSILGSFLGSV
                     LMGLILAGLTMLSVDPYWQGLVTGILIILAISMDSFQHLRSERRTL"
     gene            complement(1994..3481)
                     /locus_tag="PK171_02920"
     CDS             complement(1994..3481)
                     /locus_tag="PK171_02920"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05187.1"
                     /translation="MIRIDHVSKSFPGVIALENISFQINSGTVHGLVGENGAGKSTLI
                     KIISGIYSDYEGDIFIDDLRLQITSVHEAQKKGIATIFQELTVVRELTVAENIFLGRE
                     PVGFAGALDRAEMERKSQQVLNELNVSFSPRDRVGDLSVANQQIVEISKALVLNTQIL
                     IMDEPTSSLTEKEVQNLFSVINTLKSKGITILYVSHKLEEIFKICDAITVLRDGKHIQ
                     TSLKSATTSPEVIRMMVGRSLNMLFPPRTAQKGDLLLSVKDLTRAGEFKDISFEVHAG
                     EIVGFAGLIGSGRTELAKALFGATCPNKGEIKISNQKMKLFCHPREAISHGLVYLSED
                     RKQEGLILLLSVRENISLPIVKKISQNLFVKRKEEQNIVDSLIEKLKIKVFSRDQVVE
                     TLSGGNQQKVVISKLLLTNAQVFIFDEPTRGIDVGAKYEIYKIMNDLTADGKGIVFIS
                     SELPEILGISDRIYCMREGSIVKEYTREEANPENVMSVLTGGSSI"
     gene            complement(3609..4586)
                     /locus_tag="PK171_02925"
     CDS             complement(3609..4586)
                     /locus_tag="PK171_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT05188.1"
                     /translation="MKKGIFLVMMVIFLLSMTMIASAQELTIAVIPLSLGHPWWVRCG
                     EGAMKAGEELGINIIYTAPEKEDAAKQLDYFNDQVNKGVDAILLAAVDAETMKKPIAD
                     AIAKGIPVFGFDIGAPGTDIIWTASGWEPVQSGTNIGEGLAKEIGGKGKVALITAGLG
                     QPYLEMRQKAIEAVLAQHPDITIVGLYANDNDYEKALSQCESVLLAHPDLAGFASTTT
                     TGIPAAAQAIINAGLEGKVAVWGVSMPKQNADYVKNGIVKGGLALDSAQMTYLGVRIA
                     YDYLTKDKVLPVPGNEYGWAEVPVTDVEEKFSYVPDTLLTPENVDTFGF"
BASE COUNT         1556 a         1078 c          861 g         1366 t
ORIGIN      
        1 actgaccctt gacatttgtc atttcattca ttacctttct caagaatttt atttaatagc
       61 aacagtctga tggggtttct ttttctgcct cttccctatc aatgcctttg atattccctc
      121 tctcccaacc acatagataa gcagcaagcc taaaacgacc aactgccaaa agggatttaa
      181 atttaataaa ctaaatccgt tggttaagag gaaaagtgcc aaaacaccca gaagagaacc
      241 ggcaattgta cccttaccgc cggtaatagc tgtcccaccg atgactacag cagtcaccac
      301 ttcgagaggt gaatttaatc cggcatcggg gataatcgct cgaattcgaa tcgaaaagag
      361 gagaccagca caagcacaaa taaaaccact taaaatataa acggtatatt ttattcggtt
      421 taccgatatc ccagacagat aggctgagcg ttcgtttccg ccaacagcat agatttgcct
      481 tcctatataa gttcgttgta aaagtaatga agataaaaca gcaaaaacaa tcagtaccag
      541 aaacaagaag ggaaaaccaa gtatggttaa atcaccatag gctaagtagg attcaggaaa
      601 atggctgagg gttttccctc gaagcacacc gtaaacaata cttctaagga tggacatggt
      661 agccagagtc acgataaaag gctgtaatcg taatttgacg ataaaaagag cattcatcag
      721 tccagcagcc atgcctccta ataaagcaag caagacacat aaaaagaaag ggagtccaaa
      781 aatatacatc tgtccagcga ttaaacctga tatagccatt atagtaccaa tagataaatc
      841 aaaaccaccg cttataaaaa caatggtttc agcaatggct aacattccca actcgctcat
      901 acgtttaaga accgaaagca agttttcata ggttaaaaaa gtgggtgaca aaaaagagag
      961 aataacaata attccaacat taatcagagt aagaatgagg atcgatcgaa attgttcact
     1021 tcccggtttc atagagtcct cctctcactg cggagatgtt ggaaagaatc catagaaata
     1081 gccaatataa tgagaatgcc ggtaactaaa ccctgccaat aaggatcgac agatagcata
     1141 gtcaaaccag ccaaaattaa acccattaaa accgatccga ggaaagatcc taaaatactc
     1201 cccgctccac cggtaatgct ggtgccaccg ataactaccg cagcaatggc attcatctca
     1261 tagccaatac cggcttgagg ttggatgaaa ccacttctcc caacaaaaat taaaccagca
     1321 aaagctgaaa gaataccgct aatcgcataa acggctatct tatatttttg aaccggaact
     1381 cctgaataga tcgccgactg ttcactgcca ccaatggcat aaatattccg accaaaacga
     1441 gtaaaaaggg taatataaat aaaaagaatt actgttccca gcaaaaataa aaatggagtt
     1501 aaaccccggc caatatacaa aaattgggaa ggtatgggtg taatatattt tccttgagta
     1561 atgatcatag taattccccg gtagatgttc atcgtcccca aggtgataat cattgccggt
     1621 acggcaattc gggtaaccaa taacccgttt accaatccca gaattaatcc taatacgaga
     1681 accagaggat aaaccaccca aatcgttctc ccatcgggaa ttcccattcc tccaatacta
     1741 atggcaaaag ataagattga accaaccgaa agatcaatgc ctgcagttag aatagctaga
     1801 gtcattccca tggataagat tcctacaacc gctatctgac gtgaaatatt gatgaaattc
     1861 cttgccgaga aaaatcctgg aataacaaaa gaaaatacaa ttaacatgcc taatatataa
     1921 aggaaaatgg gtagctcacg aaaattacga agcggtctcc gtttttcctg tattttctgc
     1981 tcaagcggca tattcatatc gatgaacccc ccgtaagaac tgacatcacg ttttcaggat
     2041 tcgcttcttc ccgagtatat tccttgacga ttgacccttc ccgcatacaa taaattcggt
     2101 cggaaatgcc taaaatttct ggaagctctg aggagataaa gacaattccc ttcccatcag
     2161 cagtcaaatc gttcataatt ttgtagatct cgtatttggc tccgacatca atgccacggg
     2221 taggttcatc aaaaatgaat acctgggcgt tggtgagaag caattttgaa ataaccactt
     2281 tttgttgatt acctcccgat agggtttcaa ccacttgatc tcggctaaat actttaattt
     2341 ttagcttttc gatcaatgaa tcaacaatat tttgctcttc ttttctcttg acaaaaagat
     2401 tttgagatat ttttttaaca attggaagtg agatattttc tctcactgat aaaagtaaaa
     2461 tgaggccttc ctgcttccgg tcttcggaaa gatagaccag accgtgagaa atcgcttcac
     2521 gaggatgaca aaacagtttc attttttgat tactaatttt tatttcccct ttgttgggac
     2581 aagttgctcc aaataatgct ttggctagtt cagtccttcc cgaaccaatt aaacctgcaa
     2641 aaccaactat ttctccggca tggacttcaa agctgatatc cttgaattca ccagctcgag
     2701 tcagatcctt taccgacaga agcaaatcac ctttttgagc cgtccggggt gggaacaaca
     2761 tgttcagtga ccgaccaacc atcattcgaa taacctcagg tgaagttgta gcagatttga
     2821 gactggtctg aatgtgtttt ccatctcgca ggacagtaat ggcatcgcat attttgaaga
     2881 tttcttctaa tttatgagaa acataaagaa tagtaatacc tttacttttc agggtattga
     2941 tgaccgaaaa taggttttgg acttcttttt cggttaaaga tgaagtaggc tcatccataa
     3001 ttaaaatctg ggtatttaaa accaaggctt tgctaatctc aacaatttgc tggttggcga
     3061 ctgagaggtc tcctacccta tcacgggggg aaaaagaaac atttagttca tttaaaactt
     3121 gttggctttt cctttccatt tcagctcgat ctaaagctcc ggcaaaaccg actggttctc
     3181 ttcctaaaaa tatattctct gccacagtga gttcccgaac aacagtcaat tcttgaaaaa
     3241 ttgtggcaat accttttttt tgtgcttcat gaactgaagt tatctggagc cgcagatcat
     3301 caataaatat atctccttca tagtcggagt aaattccaga aattatttta atcaaggtac
     3361 tctttccagc gccattttct ccaactaaac catgtacagt accagaatta atctggaagg
     3421 aaatattttc gagtgcaatg actcctggaa atgacttgga aacatgatca atgcgaatca
     3481 ttggattcca ccttcgttcc ttattgctac atcattttgg atttatcttt tttcttgcag
     3541 caaaaaacag aaacggaagg ccataaataa acaaactttt atagccttcc ggtgtgaaca
     3601 gctgattctt agaatccaaa agtatctacg ttttctggag tcaatagcgt gtcaggaaca
     3661 taggagaact tttcttcaac atcagtaact ggtacctctg cccagccata ttcgtttcct
     3721 ggaacaggta gaactttatc tttggttaga taatcgtaag caattcgcac tcctaaataa
     3781 gtcatctgag ctgaatctaa tgccaatcca ccctttacaa ttccattttt gacataatca
     3841 gcgttttgtt tcggcattga aactccccaa accgctactt ttccttccaa gccagcatta
     3901 ataattgctt gagccgctgc tgggattcca gtggtggtgg tactggcaaa accagctaaa
     3961 tcaggatgag cgagtaatac tgattcgcac tgactcaagg ctttttcata gtcattatca
     4021 ttggcataca agcccacgat ggtaatatcc ggatgttgag ccaataccgc ttctatggct
     4081 ttttgtctca tttcaagata gggctgccca aggccagcag tgattaaggc aactttccct
     4141 ttgccaccaa tttcttttgc taaaccttca ccaatgttgg taccactctg aactggctcc
     4201 catcccgaag cggtccaaat aatgtcggtt cccggtgcgc caatatcaaa accaaatacc
     4261 ggtattccct tggctatcgc atcggcaatc ggctttttca tggtttcggc atcaactgct
     4321 gccaaaagaa tggcatcaac gcctttatta acttggtcgt tgaaataatc cagttgtttg
     4381 gcggcatctt ctttttctgg tgcagtataa ataatattaa taccaagttc ttctccggct
     4441 ttcatggcac cttctccaca acgtacccac cagggatgac cgagagataa cgggataaca
     4501 gcaatagtaa gctcttgagc gctggctatc atggtcatag aaagcagaaa aatcaccatc
     4561 attactaaaa atataccttt tttcacggtt tattccctcc atttttaaaa tcttttttcc
     4621 ttaatgtcgt cttaccttaa tcaactacaa cttcccaggc ttgaaaagaa tggtcttaaa
     4681 ggtcataaaa gtttttaata aatatgaaat ttgcttttta aacaggaatt ttcacctcct
     4741 cattctcctt agtttttcta atttatcttg aaaataacag aatgaatgaa aaatgatcaa
     4801 aaaagcaagg cacacccccc tgaatccccc ctcaatgggg gaataaattc aacaattccc
     4861 c
//
LOCUS       DAOSOL010000079         4840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_17605_length_4840_cov_3.610658, whole genome shotgun sequence.
ACCESSION   DAOSOL010000079 DAOSOL010000000
VERSION     DAOSOL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17605_length_4840_cov_3.610658"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PK171_02930"
     CDS             complement(<1..200)
                     /locus_tag="PK171_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05189.1"
                     /translation="MMNYLVVGMGKTGESTARFLLNRGEKVLGYDDHKKWSDISPNLL
                     NHPAFSPLKPIDLSKLNRRKIQ"
     gene            complement(205..1176)
                     /gene="mraY"
                     /locus_tag="PK171_02935"
     CDS             complement(205..1176)
                     /gene="mraY"
                     /locus_tag="PK171_02935"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOT05190.1"
                     /translation="MKLGIIPNDILIAFLLAFVVGMVFFPLFIRWQQQRHLGQKIKKE
                     GPNLHLHKENTPSMGGIVIFIALLVVFGVGRQYWLSNLLPVVLLAGYTILGLVDDWYK
                     SYLEKPWGIKARYKILFQIVLAGIVLWLAMDGMPTQIIIPFSRYVIPMSRPLFFGYGI
                     FIIIATSNAFNIADGLDGLAGGSGILTFLFWGLLLLTLGKTQLSWLAFGAIGGLTAFL
                     WFNIWPAGIFMGDSGSLGLGALLGFMALISGQSLLIIFSALVFFVDTLSVILQVFSFK
                     ILGRRLFLMSPIHHHFELKGMKETQITVRFWIIQAIGITVAWLGRVQ"
     gene            complement(1151..2566)
                     /gene="murF"
                     /locus_tag="PK171_02940"
     CDS             complement(1151..2566)
                     /gene="murF"
                     /locus_tag="PK171_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT05191.1"
                     /translation="MISKPDIIFAAGAKLVAPGKQEKFDKIMIDSRQNTECGLFVAIK
                     GKKTDGHAYLNDAWDHGARGALVSQKVNSPQEFSLYLVDDTVEALKNLGKFYSKQLTG
                     KKIAITGTVGKTTTKFYFQKILGIRFRVAATPFSYNTNIGVSASFCNFSSQNDFFIIE
                     AGINKKGEMGELAEIIDPEIVVFTAFGKGHLEGFGNVETVIQEKILLAENAKTIYLNG
                     DNIYALKLKEDFSQKGKEVILFGLQNHITSQLSLLDYHLNPTEGITRFRCQIEGRIYH
                     FQAPIFSPELIILLLPVLHQAFQLGIEEDEVQTVLNEILLPEGREKIYHLGEGYVIDD
                     SYNANPISLHKALNLLKRFNHQGYQTWVVLGDMLEMGSFSEAVHQESIRSLEEHNIKN
                     VILYGSMMHGAAEKVLSKNSVDGEYYLASSHEEIKKILLDQITFPFSRLIVLFKGSRA
                     MKLEEAIPTEWIEHETWNYSQ"
     gene            complement(2563..4068)
                     /locus_tag="PK171_02945"
     CDS             complement(2563..4068)
                     /locus_tag="PK171_02945"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT05192.1"
                     /translation="MILKKLFNLNEYLYKNIFDDQLEVSGLAFDSRLVRPGCVFFAFP
                     GANTDGHLFIEDAIKRGAKVIVYSDPSFSFPTHGKVSWIRVDDERKTMAQTAARFYDH
                     PSREMRLFGITGTNGKTSTCFFLRSILEENGHACGLMTTPKNIIGPDIYEAINTTEES
                     LQIQACLKSMIKIGIRQAVLEVSSHGLALGRVEGLLFDTAVVTNLVAEHLEFHKSFEH
                     YFFSKKKLIEMVEQNTQKPYPRMVIVNGDDENCRRVVFESHLPLLTFGLQPENEVTAE
                     DIVYFVDRTKMKIKTPWGTEKVQLSLPGQHAVYNALAAAATAISSGIESEKIAQGLEH
                     THRVPGRWEVVNAGQDFLVIVDFAHNWHGLENTLSFIKKMVQGKVITVFGCGGERDRN
                     KRPMMGETVARWSDVCIITSDNPRHEDPRQTAEDAMQGVNKYKKEIPVESYIILDRRE
                     AIDYAFSLAKTGDVVFLAGKGPERYQVYHDRIICHNDYDVALELLKERNLE"
     gene            complement(4065..>4840)
                     /locus_tag="PK171_02950"
     CDS             complement(4065..>4840)
                     /locus_tag="PK171_02950"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT05193.1"
                     /translation="SFYCGGSIMVYNHRVRDIKSHGQETLEDIIINSCNVGTIQIAQR
                     LGEDTLYRYLKNFGIGESCETGLNGEEIGLLRKPRSWSALSIGAIPIGQEMLVTPIQL
                     LRSVSAIANRGILMKARLVKKVIDENGKVVREVLPSPVQRVVDEETADLVLNMMKKVV
                     DQGTGKKAAIPGYAIAGKTGTGQKIGEDGRYAINRFYSSFIGFFPIPEPRFGILVVLD
                     EAQGEYYGGDIGAPVFREIAQNLIHYAGIMSENDEVTIY"
BASE COUNT         1481 a         1099 c          868 g         1392 t
ORIGIN      
        1 tcctggatct ttcttctatt gagcttggaa agatcgattg gcttaagcgg ggaaaaagcc
       61 ggatgattaa gaaggttcgg cgatatatcg ctccactttt tgtgatcgtc atatcccaat
      121 accttctcgc cacgattaag caaaaaacga gcagtacttt ctccagtttt tcccataccc
      181 acgactaaat aattcatcat aaccctattg aacccgccct aaccatgcca ccgtaattcc
      241 tatggcttga attatccaaa aacgtacagt aatttgagtt tctttcattc cctttaattc
      301 aaaatgatga tgaatcggac tcattaaaaa tagtcttcgt cctaaaattt taaaagaaaa
      361 aacctgtaat atcactgaaa gagtatcaac aaaaaaaacc agggctgaaa aaataattaa
      421 aagagactgc ccagaaataa gcgccataaa gcctaagagc gctcctaaac ccaatgaacc
      481 tgaatctccc ataaaaatac ctgccggcca tatattaaac cataaaaagg cggtcagacc
      541 tccgattgct ccaaaggcca accaggaaag ctgggtttta cccaaggtta ataaaagcaa
      601 accccaaaaa agaaaagtga gaatacccga acctcccgcc aacccgtcca aaccatcggc
      661 aatattaaat gcattggagg tagcaatgat aataaatatc ccgtacccaa aaaaaagtgg
      721 acgagacatt ggaattacat aacggctaaa tggaatgata atctgagttg gcatcccatc
      781 catagctagc cagagaacta taccggcaag gacaatctgg aacaaaatct tataacgggc
      841 ttttattccc cagggtttct ccaaatagct cttataccag tcatcaacca atcctaaaat
      901 agtatatcca gcaaggagta ctaccggtag taaattgctt aaccagtatt gtcttcctac
      961 tccaaacact accaaaagag ctataaatat gacgataccg cccatactgg gggtattctc
     1021 tttgtgaaga tggagattag gtccttcttt ctttattttt tgaccaagat ggcgttgctg
     1081 ctgccaccga atgaaaagcg gaaaaaaaac cattccaact acaaaagcca aaagaaaagc
     1141 aattaatata tcattgggaa taattccaag tttcatgctc gatccactcc gtaggtatag
     1201 cttcttctaa tttcatggct cttgaacctt taaacaaaac gataagtcga gaaaaaggga
     1261 aagttatctg atcgagaaga atttttttga tttcttcgtg ggaagaagcc aaataatatt
     1321 ctccgtctac cgagtttttc gataacacct tttcagcagc cccatgcatc atggaaccat
     1381 aaagaatgac attcttaata ttgtgctctt ccaaagaacg gatactttct tgatgaactg
     1441 cttcggaaaa actccccatc tctaacatat ctcccaaaac cacccaggtt tgataccctt
     1501 gatggttgaa tcttttcaat aaattgaggg ctttatgcaa agaaattgga ttggcattat
     1561 agctgtcgtc gatcacatat ccttcgccaa gatgataaat tttttctctt ccttcgggaa
     1621 ggagaatttc attaagaacc gtttggacct catcttcttc aatgcccaat tggaaagctt
     1681 gatgaaggac cggcagaagt aagatgatga gttccggaga aaaaatcggt gcttggaagt
     1741 gataaatccg accttcaatc tgacagcgga atctagtgat tccctccgtt gggttcaagt
     1801 gataatcaag aagtgagagt tgtgaggtga tatgattctg aagaccaaaa agaatgactt
     1861 cctttccctt ctgggaaaaa tcctctttga gttttaaagc gtaaatatta tccccattca
     1921 aatagatagt tttggcgttt tcagccaata aaattttttc ctgaatcacc gtctcgacat
     1981 taccaaaacc ctctaaatga ccttttccaa atgcagtgaa cacaactatt tccggatcaa
     2041 tgatttcggc taactcaccc atctcacctt tcttattgat tccggcttca atgataaaaa
     2101 agtcgttttg agaagaaaaa ttgcaaaaag aggctgacac cccgatattg gtattataac
     2161 taaagggagt tgcagccact cgaaagcgaa tgccaagtat tttttggaaa taaaattttg
     2221 tggtcgtttt cccaaccgtt ccggttatgg caattttctt tccagtaagc tgttttgagt
     2281 agaatttccc gagatttttt aacgcttcaa cggtatcatc aacgaggtaa aggctaaatt
     2341 cttggggaga attgaccttt tgactcacta aggctccacg tgcgccatgg tcccaggcat
     2401 catttaaata agcatgacca tcggtttttt tcccttttat agccacaaac aaaccacatt
     2461 cggtattttg acgactatcg atcataattt tatcaaattt ttcctgtttt cccggagcga
     2521 caagtttggc tccagctgca aaaataatat ccggtttcga aatcattcta aatttctctc
     2581 ttttagaagt tctaatgcca catcataatc attgtggcaa atgattctat catgatatac
     2641 ctgataacgt tctgggccct tcccggctaa aaaaactaca tcgccagttt tcgccaaaga
     2701 aaatgcataa tctattgctt cacgtcggtc aaggataata taagattcca ccggaatctc
     2761 ttttttatat ttattgactc cctgcatagc atcttcagcg gtttgtcgtg gatcctcatg
     2821 gcgaggattg tcgctggtaa taatgcaaac gtcactccag cgagcaacgg tttcccccat
     2881 catcggcctt ttattccgat ccctctctcc accgcaacca aaaactgtaa tgactttccc
     2941 ttgaaccatt tttttaataa aagaaagagt attttccagc ccatgccagt tgtgagcaaa
     3001 atcaacgata accaaaaaat cctgtccggc gttaactacc tcccaccttc ctggaactcg
     3061 gtgggtgtgc tcaaggcctt gagcaatttt ttcactttct attccagagg aaatagccgt
     3121 agctgccgct gctaaagcat tataaactgc atgctggccg gggagggaaa gctgaacttt
     3181 ctcagttccc cagggggttt ttattttcat ttttgtccta tcaacgaaat atacgatatc
     3241 ttcagcggtc acttcgtttt ccggctgcaa accaaaagtg agaagcggga ggtggctttc
     3301 gaaaacgact cttcgacagt tttcatcatc tccatttaca attaccatac gagggtaagg
     3361 tttttgggta ttttgttcca ccatttcgat gagtttcttc ttgctgaaaa agtagtgttc
     3421 aaaactttta tgaaactcta agtgttcagc taccaagtta gtaaccactg ctgtatcgaa
     3481 taacaaaccc tcaacccgtc ctaaagccaa gccatgggaa gaaacttcta aaaccgcctg
     3541 acgaatccct atcttaatca tacttttcaa gcaagcttgt atttgcagcg actcttcagt
     3601 ggtattaatc gcttcataga tatcaggacc aatgatgttt tttggtgtgg tcatcagacc
     3661 acaagcatgg ccattttctt caagaatact acgcaggaaa aaacaagtgc tggtttttcc
     3721 attggtacca gttataccga acagcctcat ttcccgggag ggatgatcat agaatcgagc
     3781 ggcagtttga gccatggtct ttctctcatc atcaactcga atccatgata ccttcccatg
     3841 agtaggaaaa gaaaaactgg gatcggaata gacaatgacc ttcgctcccc ttttaatggc
     3901 atcttcaata aagagatgcc catcggtatt agcaccggga aaagcaaaaa atacacaccc
     3961 tggacgaacc aatcgagaat caaaagccaa tccgctgacc tctaattggt catcaaaaat
     4021 atttttatag agatattcat ttaaattgaa cagttttttt aaaatcaata gatcgttacc
     4081 tcgtcatttt ccgacataat accagcataa tgaataaggt tttgagcgat ttcccgaaaa
     4141 accggtgctc cgatatcgcc tccatagtat tcaccctggg cttcatctaa aaccaccaat
     4201 attccaaaac gtggttcggg gattggaaaa aatccgataa aggaagaata aaaccgatta
     4261 attgcataac ggccatcttc tccaattttt tgtccggttc cggttttccc cgcaatagca
     4321 tatcctggaa tggcagcttt tttacccgtt ccctgatcaa cgactttttt catcatgttt
     4381 aaaaccaaat cagcagtttc ttcatcaaca accctttgaa ccggtgatgg gagaacttct
     4441 cgaacgacct tcccgttttc atcaatgact ttttttacta atcgggcttt cataagaatc
     4501 ccacgattag ctatggccga tactgatctt aagagctgaa ttggggtgac cagcatctct
     4561 tgaccaatgg ggatggcacc gatggatagc gccgaccagc tgcgaggctt cctcaataaa
     4621 ccaatttctt ctccattcaa accggtttca caggactctc cgataccaaa atttttcaag
     4681 tagcgataaa gtgtatcttc tcccagtcgt tgggcgattt gtattgtacc gacgttacag
     4741 gaattaatga tgatatcttc gagtgtttcc tgcccatgac tttttatgtc tcgcactcga
     4801 tgattataca ccattatgga accaccacaa taaaaacttt
//
LOCUS       DAOSOL010000080         4806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_17747_length_4806_cov_3.624079, whole genome shotgun sequence.
ACCESSION   DAOSOL010000080 DAOSOL010000000
VERSION     DAOSOL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17747_length_4806_cov_3.624079"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..2607)
                     /locus_tag="PK171_02955"
     CDS             complement(196..2607)
                     /locus_tag="PK171_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT05194.1"
                     /translation="MNFEQFTEKAKEVIHLAQNILLEYHHNQLDVEHILLALVKQPEG
                     ITGQILERCQVSPETIQNELETSLEKRPSVYFQASTEAQIYITPRAKSVLDGAVEEAK
                     RLKDSYVGVEHLLIAVAKEESGLSNKILKKYKVDVEKIYRALQEIRGKRKVDDPYAEG
                     RYMALEKYGRDLTQYAQDGKLDPVIGRDNEVNRVIQILSRRTKNNPVLIGDPGVGKTA
                     IVEGLAQKIVQGNVPESLKGKRVIILDMGSLLAGAKFRGEFEERLKAVLEEVQKSEGK
                     IILFIDELHTVVGAGAAEGAIDAANILKPLLARGELQTIGATTLDEYRKYIEKDAALE
                     RRFQPVFVGEPSIEETKKILQGLRDKYEAYHKVKIADDALDAAAELSAKFINDRFLPD
                     KAIDLIDEAASKKRIDIQSVPPEIKDMELELQKMAQEGMAAVQAQEYEKAAALRDQAE
                     ALRLRYQETRDKWLQEKQMSEVVSASDIAEVVSSWTGIPTTQLMEKERERLLRMEEEL
                     HRRVIGQDEALHAVAEAIRRSRAGITEGKRPIGSFLFLGPTGVGKTELAKALAEFMFG
                     SDDALLRIDMSEYMEKHTVSRLIGAPPGYVGYEEGGQLTEIVRRRPYQVILLDEIEKA
                     HPDVFNILLQILDDGRLTDGQGRTVDFRNTVIIMTSNLGSQAFRSLSITELAGFEKSK
                     QAVLKEVERHFKPEFINRIDEIIVFKPLTEEQIHQIVDIMMSYLEKRLQEQGLSIVLD
                     DQVKDFLAKNGYDPDYGARPLRRLIQRKIESPLASELIRCTFQPGQTIQVKMGPDQQI
                     QFSCE"
     gene            complement(2604..2954)
                     /locus_tag="PK171_02960"
     CDS             complement(2604..2954)
                     /locus_tag="PK171_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT05195.1"
                     /translation="MVKNNQKDEYFIISVVAQMTNLHPQTLRYYEKMGILRPNRSQGN
                     IRLYSYQDVERVRQIKALTQDMGVNLAGVEIILRLTEQIEAIQGQWEKEREKMRNRIK
                     DLEGRISKGGEILL"
     gene            complement(2991..4037)
                     /locus_tag="PK171_02965"
     CDS             complement(2991..4037)
                     /locus_tag="PK171_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOT05196.1"
                     /translation="MEFKDYYQILGLNRNADEKEIKKTYRRLARKYHPDLNPGNKESE
                     KRFKEINEAYEVLGDPEKRKRYDELGSAWNSYGQKDTEQFWKDYYNKYGSGGTSYQQT
                     YSTNFEGGDFSDFFRTFFGDLFGGSSSRTHSTRFRSSRKNEWINGYPQKGEAEPSSHP
                     IEINFIESVLGTRKNFHLEFEEPCPQCRGQNQNCTTCNGRGIVRRKKTVDVSIPSGIQ
                     DGSKLRVPGVLNGRDLYLVVKIQPHPFFRREKHDIHLDLPLTLYEALLGTEIEVPTVN
                     GKVKMKIPPETQNGMTLRLRGLGIKDRKTGSVGDQLVKIRVVLPTRLDEKERKLFQDL
                     SSIRKENPRSHLFT"
     gene            complement(4161..4607)
                     /locus_tag="PK171_02970"
     CDS             complement(4161..4607)
                     /locus_tag="PK171_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT05197.1"
                     /translation="MVIIVNRFDPFRDLTLLQDRINRLFDDSMVRHERQPSPNEAWVP
                     MVDITEDENEIVLWVDLPGVSQKEVDIIINGDQLTLKGEKKFDQTNQRKYLRRERLVG
                     PFSRTFQLNLPVETEEISAAYRNGVLEIHLPKPAESKPRKVSIQTE"
BASE COUNT         1281 a         1068 c          811 g         1646 t
ORIGIN      
        1 ccttacccaa gatttaaaaa aaggaatggg gcgctatgta ttgcgcccca ttcatggatt
       61 ttattctttt tgttgccact aagtggcatg aacgtctaat ctttaagcac agcaataaac
      121 aaaaataccc agaaaagtta tttgggaacc tctctcccca attgaatact tcaattaaat
      181 cgatcaatcc tatcgctatt cacaagaaaa ctgaatttgt tgatctggac ccattttcac
      241 ctgtatggtt tgtccgggtt ggaaagtaca acggattaat tcactggcta atggactttc
      301 tatttttctc tggattaatc gtcgtaacgg acgagcacca taatcaggat catatccatt
      361 ttttgctaaa aagtccttca cttggtcatc caaaacaata ctcaagcctt gttcctgaag
      421 ccgtttttca aggtaagaca tcattatgtc aacaatttga tgaatttgtt cttctgttaa
      481 gggtttgaac acaatgattt catcaatacg attaataaat tctggcttga aatgtctttc
      541 aacttcctta agaactgctt gtttcgattt ctcaaatcct gctaactcag taatggaaag
      601 acttcggaat gcctggctgc ctaaatttga agtcataata ataactgtat tacgaaaatc
      661 aaccgttctc ccttgtccat cagttaatcg tccgtcatcc aaaatttgta ataagatatt
      721 aaacacatcg ggatgagctt tctctatttc atcgagaaga ataacttgat aagggcgtct
      781 tctcacgatt tcggtcagtt ggccaccctc ttcatatcct acataaccag gaggtgctcc
      841 aattaaacgg gatacggtat gtttttccat atattctgac atatcaatcc gtagcaaagc
      901 atcatcgctt ccgaacataa attccgccag agcttttgcc aattcagttt tcccaacacc
      961 ggtaggtcca agaaagagaa aagagccgat tggtcttttc ccttcggtta tgcccgcccg
     1021 ggatcgtcga atcgcttcag caaccgcatg aagagcttca tcctgtccaa ttactcgccg
     1081 atgaagctct tcttccatac gaagcaacct ttctctttcc ttttccatca gttgagtagt
     1141 tggtatgcca gtccaggaag aaaccacctc agcgatatcg gaagctgaaa cgacctcgct
     1201 catttgtttt tcttgaagcc acttatcccg agtttcttga tagcgaagac gaagcgcttc
     1261 ggcttgatct ctcaaagcag cagctttttc gtattcttga gcctggactg ccgccatccc
     1321 ttcttgagcc attttctgca gctcaagctc catatccttt atttctggtg gaacactttg
     1381 gatatcaata cgctttttac tagcagcttc atctattaaa tcaattgctt tatcaggaag
     1441 gaagcgatcg ttaataaatt ttgccgataa ttcggccgca gcatcaaggg catcatctgc
     1501 tattttcact ttatggtagg cttcatattt atcccttagt ccttgaagga tttttttggt
     1561 ttcttcaata ctaggctcgc ctacaaaaac cggttgaaaa cgcctctcca aggctgcatc
     1621 tttctcgata tattttcgat actcatccag agtagtagct cctattgttt gcagctctcc
     1681 tcgagctaat aaaggtttca ggatgttcgc agcatcaatg gctccttcgg ctgctccagc
     1741 acccacaacg gtgtgcagtt catcaataaa tagaattatt ttcccttccg acttttgaac
     1801 ttcctctaaa acagctttca aacgttcctc aaattctcct cggaactttg cacctgccaa
     1861 gagagaaccc atatcaagaa taataacacg ttttcctttt aaactttctg gcacattccc
     1921 ttggacaatt ttttgtgcca aaccttctac gatagctgtt tttcctactc cagggtctcc
     1981 aataagaact ggattatttt tggtccgtcg gcttaatatt tgaattactc tattcacttc
     2041 gttatctcgt ccgattaccg ggtctagctt cccatcttga gcatattggg ttaaatctct
     2101 tccatatttt tctaaggcca tataacgtcc ctccgcataa ggatcatcaa ctttcctttt
     2161 tcctcgaatt tcttgtaggg ctcggtatat tttttcaaca tctaccttat atttttttaa
     2221 aatcttattg cttaaaccac tttcctcttt agccacagca attaaaagat gttccacgcc
     2281 aacataggaa tcttttaacc ttttggcctc ttcaaccgct ccgtctaaaa ccgattttgc
     2341 tcgcggtgtt atataaatct gagcttcagt gcttgcttgg aaataaaccg agggtctttt
     2401 ttctaaagag gtctccaatt cattttggat ggtttccgga gaaacttgac aacgttctaa
     2461 tatttggcca gttattccct cgggctgctt aactaaagct aaaagaatgt gctctacatc
     2521 cagttgattg tgatgatatt ctaataagat gttttgagct aaatgaatga cttctttggc
     2581 tttttcggta aactgttcaa agttcataac aaaatctcac cacccttcga aattcttccc
     2641 tctaaatcct tgattcgatt tctcattttc tctctttctt tttcccattg accttgaata
     2701 gcttctattt gttcagttaa tctcaggatt atttcaactc cagctaaatt gactcccata
     2761 tcttgagtta gagctttgat ctgtctcact ctttcaacat cttgatagga atacaaccgg
     2821 atatttcctt gacttcgatt aggtctaagt attcccattt tttcataata ccggagagtt
     2881 tgaggatgca ggttggtcat ttgagcaacc acactgatta tgaaatattc atccttttgg
     2941 ttatttttta ccatcttaca tacatcctcc tctattccta ttaatcgact ttatgtgaac
     3001 agatgagatc ggggattttc cttccttatt gacgataaat cttgaaataa tttcctttct
     3061 ttttcatcaa gacgggtagg caaaacaact cgaattttga ccaattgatc gcctactgat
     3121 ccagtttttc tatccttaat ccccagccct cgaagccgca aagtcattcc attttgggtt
     3181 tcaggaggga ttttcatttt cacttttcca ttaacggttg gcacttcaat ttcagttccc
     3241 aaaagagctt cgtatagagt taatgggagg tcaagatgga tatcgtgctt ttctctccta
     3301 aaaaagggat ggggttggat tttaaccact aaatataaat cacgcccatt gagcactcca
     3361 ggtactctta atttacttcc gtcttgaatt cctgagggga tacttacatc aacggttttt
     3421 tttcttctga ctattcctcg accattacag gtagtacagt tttgattctg gcctctacat
     3481 tggggacagg gttcttcgaa ttcaagatga aaattctttc gcgttcctaa aacactctct
     3541 ataaaattaa tttctatggg atgggaagaa ggctctgctt cacctttctg aggatatccg
     3601 tttatccatt catttttcct cgaagatcgg aaacgagtcg aatgggttcg agaactggat
     3661 cctccaaata aatcaccaaa aaatgttcgg aaaaaatcgc taaaatctcc tccttcaaag
     3721 ttggtagaat aggtttgctg gtatgaggtt ccacccgatc catacttgtt atagtaatct
     3781 ttccaaaatt gctcagtatc tttttgccca taactattcc aagccgatcc cagttcatcg
     3841 tatctttttc gcttttctgg atcacccagg acttcatagg cttcgttaat ttctttaaag
     3901 cgtttttcgg attctttgtt tccgggattg aggtcgggat gatacttacg agccaatcga
     3961 cgataggttt tctttatttc tttctcatcg gcatttctat ttaaacctaa tatttggtag
     4021 tagtctttga attccattga aatttcatcc cctttaccgg aaattctctt ttttaaacta
     4081 cttaacacaa agaagggatg gttttaacca tcccttcttt tcttatctct cataatctcc
     4141 tcacagctga ttgattagga ttattcagtc tggatggaaa ccttccgagg ttttgattcc
     4201 gccggcttgg gaagatgtat ttctaagacc ccattgcgat aagccgccga tatttcttcg
     4261 gtttcaaccg gtaaattaag ttgaaaagtt cttgaaaaag gacccaccag tctttctctt
     4321 cgtaagtatt ttctctggtt agtttggtca aacttctttt caccttttag ggtaagttga
     4381 tcaccgttaa ttatgatatc aacctctttt tgacttactc ctggtagatc tacccaaaga
     4441 acaatttcat tctcatcttc ggttatgtct accattggaa cccaagcttc attcggtgaa
     4501 ggttgtcttt catgtcgaac catactgtca tcaaatagtc gatttatcct atcctgtaaa
     4561 agagttaaat cacgaaaagg atcaaaacgg ttcacaataa tcaccatacc tttctttgat
     4621 gttgtttgac tttgacattt aaaagtataa aacttgataa actgattgtc aagagtacga
     4681 gggtataaat tttaagtgag ggaaaaatat ggcttataaa cccaattgag gtgatatttt
     4741 ttgcatagcc tgcaaggcaa tcaataaaaa ttcattcaag cttaactgca gatttttgca
     4801 gctttg
//
LOCUS       DAOSOL010000081         4739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18057_length_4739_cov_4.877028, whole genome shotgun sequence.
ACCESSION   DAOSOL010000081 DAOSOL010000000
VERSION     DAOSOL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18057_length_4739_cov_4.877028"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="PK171_02975"
     CDS             complement(<1..341)
                     /locus_tag="PK171_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOT05198.1"
                     /translation="MLKPGKVEKIEGEKAWVRMQKGTSCGEHHCPLGSTLLDDSGTDF
                     YKVFAKNEISASIGSNVMVEVKDDLALKISFLIYLMPILLVLGTYLIVKALTPQVMLI
                     GVVTIIAMLIS"
     gene            complement(391..1062)
                     /gene="radC"
                     /locus_tag="PK171_02980"
     CDS             complement(391..1062)
                     /gene="radC"
                     /locus_tag="PK171_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOT05199.1"
                     /translation="MKIKELPDFDRPREKLEKLGPKALSNIELIAVLLGRGTRVKDVY
                     QLSKEVLECVENDFEKLNFATLMKINGLGKTHACQVLAAFEISQRYFFKDGIKIQNAN
                     DVYKLVSDLKEKKQEYFVTLTLDGAARLIQKRVVFIGTLNQSLVHPREVFADAIGDRA
                     AGVICVHNHPSGNIQPSREDIAVTKKLVEAGKIIGIRIMDHVIIGKTSYFSFQAEGLL
                     NNGEY"
     gene            1742..2791
                     /locus_tag="PK171_02985"
     CDS             1742..2791
                     /locus_tag="PK171_02985"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOT05200.1"
                     /translation="MIKAVSLFSGGLDSLLATQIIMDQGIEVIAVNFIIPFANNKKMK
                     EKQYYARNMSQKMGIELVEIELRENFLDLLRNPKYDFGKNLNPCIDCKILMITQAYQV
                     MKEKKASFIVTGEVLSQRPKSQFLWGLSIIDRDSHIKGLILRPLSAKLLQETIPEQKG
                     WVDRDKLYNFSGRVRGPQFDLAQKMGITDYPAPAGGCLLTDPIFARRVYDLLIHDELT
                     MENIELLKLGRYFRLSNQFIFLVGRNEKDNLALFSQAKPEDYIFTPSHTKGPTGLGKG
                     MIDSDTEQFASGIIAYYSSSLVEPLYIKIQHQSYSSPKMVQPITLSLDQIEKYRIENF
                     PIKIIRQNNQKTPQK"
     gene            2865..3776
                     /gene="cysK"
                     /locus_tag="PK171_02990"
     CDS             2865..3776
                     /gene="cysK"
                     /locus_tag="PK171_02990"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOT05201.1"
                     /translation="MKIAQNITQLIGNTPLVRLSKLGANLPGEVVAKLEYLNPCGSVK
                     DRIGVSMINEAEKNGSIDKNTLIIEPTSGNTGIALAFVCAQRGYKLILTMPESVSVER
                     RKILSWFGAKLVLTPSEEGMRGSIKKAEELAKENANSFLPMQFKNPANPKIHRETTAE
                     EIWTDTDGLIDILVCGVGTGGTLTGTAEVLKDRKPSLKVVAVEPKKSAVLSGGTPASH
                     RIQGIGAGFIPEVLRRELIDEVIPVEDEDAFSTAKCIAQIEGIHVGISSGAAGWAALE
                     LAKREENQGKLIVAIFPDTGERYLSVW"
     gene            3784..4611
                     /gene="larE"
                     /locus_tag="PK171_02995"
     CDS             3784..4611
                     /gene="larE"
                     /locus_tag="PK171_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HOT05202.1"
                     /translation="MDNIIKIKYDKLLQILQDLKNCLVAYSGGVDSTFLLFTSHEVLG
                     DKTSGILIDTPFLPKREKSFALKTASQLNLPLIVQEVNLLQYSSIIRNQPNRCYFCKK
                     ILFETIEAVAIQKNFQFIIEGSNTDDLNDFRPGRKAIQEMNILSPLLETGFTKEEIRQ
                     ISKEKNLPTWNKPSFSCLATRIPYGTDITSEHLSRIEKAELYLSELGFEQLRVRDHYP
                     IARIEVPLMDNDLTRIHDYRHQIVERLKSFGYHWVTLDLEEYRSGNMNKELNPEELM"
     gene            4608..>4739
                     /locus_tag="PK171_03000"
     CDS             4608..>4739
                     /locus_tag="PK171_03000"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HOT05203.1"
                     /translation="MKTKKILVAMSGGVDSSVAAFLLKKQGYQVFGITMAFQVTHGSS
                     "
BASE COUNT         1534 a          902 c          863 g         1440 t
ORIGIN      
        1 atcgagatta acatagctat tatggtaaca accccgatca acataacttg aggggttaaa
       61 gctttaacta taagatacgt acctaaaacc agaagaattg gcatgagata aatcagaaaa
      121 gatattttta aggcgaggtc atcctttacc tcgaccatga cattggaacc aatcgaagcc
      181 gatatttcgt tttttgcaaa taccttataa aaatcagttc ctgagtcatc caatagcgtt
      241 gaacccagag gacaatgatg ttctccacag cttgttcctt tttgcattcg aacccaggct
      301 ttttctcctt caatcttttc aaccttacct ggttttaaca tactattcct ccaaaatggg
      361 aaaaaaccgg aatttcaatc tgatttggat ttaatattct ccattattta atagaccttc
      421 agcttggaag ctgaaataac ttgttttccc aataataaca tgatccataa tccgaatacc
      481 gataattttt cccgcttcaa caagtttttt ggtaacggct atatcttcac ggctgggttg
      541 aatattccca gatggatgat tatgaacaca tataacacca gcagcacgat ctccaatggc
      601 atcagcgaat acttcccgag gatgaaccag gctttggttt aatgttccaa tgaaaaccac
      661 tcttttttgg atgagtcgag ctgctccgtc caaagtaaga gtaacaaaat actcttgctt
      721 tttttctttt aaatcactta ctaatttata aacatcatta gcattttgta tttttatgcc
      781 atctttaaaa aaatatcttt gagagatctc aaaagcagca agaacttggc aggcatgagt
      841 ttttcctaat ccattaattt tcatcagagt cgcaaagttt aatttttcaa aatcattttc
      901 cacacactca agaacttctt tagaaagttg atatacatct ttcacccggg tgcctcttcc
      961 aagaagtacc gcaatgagtt caatatttga taaagcttta ggacctaatt tttctaattt
     1021 ttcacgaggt cgatcaaaat cgggaagttc ttttattttc atgtcatgat ctgctccatt
     1081 tatttatttt ggtcatatta tagacaattt tcaagcgttt cccaaccttc tttcttgaca
     1141 acctcagtat aaaacctata atattagtgt ttttataata ttagtaacac ctatcttgaa
     1201 aatagcatca aatgaattct ttcatccgtt atcctgagcc ttcgccttat gaaagcgtga
     1261 ggatctcatc ttttaatttt tttaaaagaa atacgaaatg agattgccac gtcgttcaat
     1321 caaaaaacgg ttgacttcct cgcaatgacg gtttgagata ggtattttca tcctcatctg
     1381 gtgctattta tgcagcatga gggtctattc tgaaaatacc aatattggtg aaaaaagaat
     1441 aaacaaaaaa agaaaaaggc acacccccct gaatcccccc tcaatggggg aatgaattca
     1501 acaattcccc tccttggagg ggtgccgttt tacggtgggg agggtgcctt tcgaattttc
     1561 tttgatacct tgagaaaatc acctagaaaa aagaataaaa agatgagatc cttacatcat
     1621 ccagcaaaaa ccacggactt cttagaataa aagaatagtg aaatgttccc gaataatttt
     1681 gaatcaacga gcattttatc aattcgaatt aaaacgttcc atccttttga ggtgttattt
     1741 tatgattaaa gcggtttcgt tattttcagg aggccttgat agcttgcttg caacccaaat
     1801 cattatggat caaggtatcg aagtcatagc ggttaacttc attattccct ttgccaataa
     1861 taaaaaaatg aaagaaaaac aatactatgc ccgtaacatg tctcaaaaaa tgggtattga
     1921 attggtagaa atagaactta gagaaaactt tttagatctt cttcgaaatc caaaatatga
     1981 ttttggtaaa aacttaaacc cttgtatcga ttgtaaaatt ctaatgatta cccaagctta
     2041 tcaagttatg aaagaaaaaa aggcttcatt tattgtaact ggcgaagtcc tctctcaaag
     2101 accaaaatct cagttcctat gggggttgag catcattgac cgagattctc atataaaagg
     2161 tttaatttta aggccccttt cagcgaaatt attgcaagag accattcccg aacaaaaagg
     2221 gtgggttgat cgggataagc tctataactt ttccggtcgt gttcgtggac ctcaattcga
     2281 cttggctcaa aaaatgggaa ttactgatta tccagctcca gccggtggat gtcttctcac
     2341 cgatccgatt tttgctcgaa gggtctatga cctgcttatc catgatgaac tgacgatgga
     2401 aaacattgaa cttttaaaat taggcagata ttttcggttg agtaaccaat ttatattttt
     2461 agttggtcgg aatgaaaaag ataatttggc attgttcagc caagctaaac cagaagacta
     2521 tatttttact ccttcccata ctaagggacc aaccggctta ggaaagggaa tgatcgactc
     2581 tgatactgaa caatttgctt caggaattat cgcttattat tcttcatctc ttgttgaacc
     2641 actctatatc aaaatacagc atcaaagcta ttcatcgcca aaaatggttc aacccataac
     2701 cttatcactg gatcaaatag aaaaataccg gattgaaaat ttccctatca aaattattcg
     2761 tcaaaataat caaaaaacgc ctcaaaaata acttgctcac caattttgtt tcagagtgat
     2821 aacattatgg tagatttttc acttgacgga aaggaagaat ttttatgaaa attgcccaaa
     2881 acattactca gctcatagga aatactcctt tagttcgttt gtcaaaattg ggtgccaatc
     2941 tacccgggga ggtagttgcc aagcttgagt acttaaaccc atgcggaagc gtgaaagatc
     3001 gtattggagt gtcaatgata aacgaagctg aaaaaaatgg ttcgatagat aaaaatacct
     3061 tgataatcga accaaccagc gggaataccg gtatagcttt ggcttttgtt tgtgctcagc
     3121 gtggctataa attaatactc actatgccag aaagcgtatc cgttgaaagg agaaaaattc
     3181 tttcttggtt tggtgctaaa ctggttctca ctccttcaga agaaggaatg cgaggatcga
     3241 tcaaaaaagc agaagaattg gcaaaagaga atgctaattc ttttttgcca atgcaattta
     3301 aaaatcctgc caatccaaaa attcatcgag aaacaaccgc cgaagaaatc tggactgata
     3361 ccgatgggct tattgatatc ttggtctgtg gagtaggaac cggaggaacc ctcacaggaa
     3421 ccgctgaggt tttaaaagat cggaaaccgt cccttaaggt agttgccgtt gaacccaaaa
     3481 agtcagcagt attatcagga ggaaccccag cttctcatcg tatccagggt attggagccg
     3541 gcttcatccc tgaagtgtta cgtcgggaat tgatcgatga agtcattcca gttgaggatg
     3601 aagacgcctt ttcaacggca aaatgcattg ctcaaatcga aggtatccat gttggcattt
     3661 caagcggagc agccggttgg gcggcacttg aattggcaaa acgcgaggaa aatcaaggga
     3721 agctgattgt agctattttt cccgacactg gagaacgata tttatcagtt tggtaaaaaa
     3781 ttcatggaca atattattaa aattaaatac gataaattac tacaaatttt acaagacctc
     3841 aagaattgtt tggttgctta ttccggtggt gttgatagta cttttttgct ttttaccagt
     3901 catgaggtat tgggagataa aaccagcggg attctgattg atacaccctt tttgccaaaa
     3961 agagaaaaaa gcttcgctct caagaccgcc agccaactga atcttccctt aatagttcaa
     4021 gaggtaaatc ttcttcaata ttcttcaatt attcgaaatc aaccaaaccg ttgttatttt
     4081 tgtaaaaaaa ttctttttga aactatagaa gcagttgcca ttcagaaaaa ttttcaattc
     4141 atcatcgaag gttcaaatac cgacgaccta aatgattttc gtccgggaag aaaagctatt
     4201 caagaaatga atattttaag ccctttattg gaaactggct ttaccaagga agaaattcgt
     4261 caaatatcga aagaaaagaa cctaccaacc tggaataagc cctctttttc ttgtctagcc
     4321 actcgtattc cttatggaac agatataact tctgaacatt tgagtcgaat cgaaaaagca
     4381 gaattatatc tgagtgaatt gggttttgag caattgcggg taagggatca ttatccaatt
     4441 gctcgaattg aagttccctt aatggataat gatctgactc gaattcatga ttatcgccac
     4501 caaatcgtcg aaagattaaa atcctttgga taccattggg tcacattaga tcttgaggaa
     4561 tatcgttctg gaaacatgaa taaagaactc aacccagagg agttaatgtg aaaactaaaa
     4621 aaatattagt cgctatgagt gggggagtcg attcttcggt tgctgctttc cttttaaaaa
     4681 aacaagggta tcaagttttt gggattacca tggcttttca ggtaactcac ggttcttcg
//
LOCUS       DAOSOL010000082         4734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18080_length_4734_cov_3.514426, whole genome shotgun sequence.
ACCESSION   DAOSOL010000082 DAOSOL010000000
VERSION     DAOSOL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18080_length_4734_cov_3.514426"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PK171_03005"
     CDS             complement(<1..540)
                     /locus_tag="PK171_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05204.1"
                     /translation="MKKLTIVFVLLFLLISISIVWAQEEKINIVHYHWTQPPYDEINA
                     RAAKTFMEKYPNVNIEIIFIADPDMPTKVRTAALGGGGMDTFAMPNMQSAWFMANGIC
                     AEIMPSAFGKETIEEVLEMWQEGSLQKTGGFYNGKYYGIPFEMSSYVAWINTGHMKEA
                     GLDPVANLPKTWKEFVDTCS"
     gene            complement(784..1815)
                     /locus_tag="PK171_03010"
     CDS             complement(784..1815)
                     /locus_tag="PK171_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT05205.1"
                     /translation="MSKFKFSVGPWNVHEGADAFGPPVRESIPLNEKIKKFKEIGFDA
                     VQFHDDDVVPEMNDLSDAAIKQKAKEVKKLLEDNGLEAEFVAPRLWMDPRTIDGGFTS
                     NHPKDREFALWRALRSVDIANELGCNKLVLWLAREGTLCYESKDPILSVNRIVEAINK
                     MLEYDKNIRVLIETKPNEPIDRSFCPTIGHAIALSYKTIDPDRVGALLESAHAILAGL
                     DPANEIAFGLANKKLWSVHLNDQNGIKYDQDKTFGVENLRQAFNQIKILVENNYGRNG
                     EYVGLDVKAMRTQKIEDIYRHLYNSLKIAIILEDKANKFDYTFQKKCVEERNYEALEM
                     YVLELIMKG"
     gene            2191..3198
                     /locus_tag="PK171_03015"
     CDS             2191..3198
                     /locus_tag="PK171_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT05206.1"
                     /translation="MKRFKFLLFLSTVLLLTFSLFVVAEAEDTDFEIVMVVKLEGVAW
                     FDNMRLGVEEFAKETGVNAYQIGAATADPASQIALIEDLIAKKVDAIIVVPNDPVALE
                     PVFKKANDAGILTFTHEASNQKQVTFDIEAFDNDAYGRHMMDVMAEDLNLEGEYACFV
                     GHLTSTTHNEWVDAEIAQQQEKYPNMKLVTDRIEEQENQQIAYEKTLELLRTYPNLKG
                     VMGSAMSTVPGAALAIEEKGLIGKVGAYGTCLPSVAYDYLKNGAAKSIHFWIPADAGY
                     VSCKVAWEVLKGNEIKEGMDLGKPGYESITIRNNQHGFPVIYGQAWHDVTAENVDDYD
                     L"
     gene            3348..>4734
                     /locus_tag="PK171_03020"
     CDS             3348..>4734
                     /locus_tag="PK171_03020"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05207.1"
                     /translation="MAEDFLRIQNVSKLYAGVQALDNVSMSIGQGEVHCLVGENGSGK
                     STLIKIIAGVVKPDGGEIVIQGKSYKNLRPIDSINEGIQVIYQDLSLFPNLSVAENIS
                     LNQLIEEKKKIINWKEVKSIASNVLLTIKKELDLKEKVENISIANKQLVAICRALLRN
                     AKLIIMDEPTTAITKKEIDKLLSVILDLKESGISTLFVSHKLSEVLQIAEKVTVLRDG
                     KKVGDYNAGELDYDSLSFYMSGKKIDHSVFDYQEGADQKTLLEIKDLSKKSQFEDISF
                     KLRPGEIIGMIGSLGSGRTEVALSLFGLNKPDSGSIYMNGEPVKINSPRKAISLGLCY
                     LPEDRLNQGLFLKQSIQNNVVVTNLKKLLTKSKLLNYSKKYEYAKTWIDELNVNVPSL
                     EIAVQALSGGNQQRVVIAKWLATNPKVFILDSPTVGIDVNSKSNIHNLIRQLAQKGIG
                     VIMISDEVPEIV"
BASE COUNT         1470 a          884 c          907 g         1473 t
ORIGIN      
        1 actgcaggta tcgacaaact ctttccaggt tttgggtaag ttggcgactg gatctaaacc
       61 agcttctttc atgtggccag tattaatcca tgccacataa ctactcattt caaaaggaat
      121 gccataatat ttcccgttgt aaaaacctcc ggttttttgt aatgagcctt cttgccacat
      181 ctccagaact tcttctatgg tctctttacc aaacgccgaa ggcattattt ctgcacatat
      241 accattagcc atgaaccagg ctgattgcat gttgggcatg gcgaaagtgt ccattcctcc
      301 tcccccaagg gccgcggttc tgactttggt aggcatatca ggatcggcta taaaaatgat
      361 ttctatgttg acattggggt atttttccat aaaggttttt gcagcacgag cattaatttc
      421 atcataagga ggctgggtcc agtggtaatg aacaatatta atcttttctt cttgagccca
      481 tactattgaa atcgatatca acaaaaacaa taagacaaaa actatcgtca attttttcac
      541 ttattgtaac cctccttcat tttttaaatt cttacaaatt cataaacaca aaaactcctt
      601 taataccaaa aaacagcctc ctttctcaaa gaattagaaa aaattaaaaa ttccggcaat
      661 aaatttatat tgatcctctt cctcgcctcc tccctttctc tttttttctt tcccatcacg
      721 agagaggaaa aaaatgaggg gggaaccgat ttaatctgta tcatatataa ccagaaccgt
      781 tatttaccct ttcataatca gttcaaggac atacatttcc agtgcttcgt agtttctctc
      841 ctcaacgcat ttcttttgga aagtataatc gaatttgttg gctttgtctt cgaggattat
      901 agcaatttta agactattgt aaaggtgacg gtaaatatct tcgatttttt gggttctcat
      961 ggcttttaca tcgagaccaa catactctcc attcctacca tagttatttt cgactaaaat
     1021 ttttatctga ttaaatgcct gtcttaagtt ttctacaccg aaggttttgt cctgatcata
     1081 cttgatacca ttttgatcgt tgagatgaac actccaaagc tttttgttgg caagtccaaa
     1141 ggcaatctca ttggctgggt cgagacccgc taagatggca tgagcacttt ctaaaagagc
     1201 gccgactcga tcggggtcaa tggttttata tgataaagca atcgcatggc caatggttgg
     1261 acaaaaactt ctatctatag gctcgtttgg cttggtttca atgagtactc gaatattctt
     1321 atcatactcg agcatcttgt ttatagcttc aacaattcga ttcactgaaa gaatcgggtc
     1381 tttgctttca taacaaaggg tgccttctcg ggctaaccaa agaaccagtt tgttgcatcc
     1441 tagttcattg gcaatatcta ccgatctcag tgctctccat agagcaaatt ctcgatcttt
     1501 aggatggttt gaagtgaaac ccccatcaat tgttctggga tccatccaca gccggggcgc
     1561 gacaaattcg gcttctaatc cgttgtcctc taataatttt ttgacttctt ttgctttttg
     1621 tttaatggct gcatcggaaa gatcgttcat ctctggtacg acgtcatcat catgaaactg
     1681 tactgcatca aaaccaattt ctttaaattt cttgatcttt tcatttaacg gaatgctttc
     1741 tcttactggt gggccaaagg catctgctcc ctcatgaaca ttccagggac caactgaaaa
     1801 tttaaatttt gacacgtcaa tatccccttt cttattcaat tttatcatga tacttctatt
     1861 tattctaaat aaatgaaaaa attatataag tttcgttcgg gtttaaaacg ttttggccaa
     1921 atttccttaa gaaaaattga ctttaaaaat tgaattggga taaaatgagg ttgagttttg
     1981 gttatttatt tttataggta ttctttaaaa aattataagc ttacttagga gaaaggaggt
     2041 actttttgta attttagcca ggacacctca atcctgagaa tggaggtgag agaggaaatt
     2101 atttagtacg tatctatccg aaaacttttt ggaattttct tgcaaataca gtaacaaaat
     2161 atttttcata actttctagg gaggatgaaa atgaaaagat ttaaattttt attgtttctt
     2221 tctactgtat tattgttaac cttttcccta tttgtcgttg ccgaagctga agatactgat
     2281 tttgaaattg ttatggttgt caaacttgaa ggtgttgctt ggtttgataa catgagattg
     2341 ggcgtagaag aatttgccaa ggaaaccggc gtaaacgcct atcaaattgg tgctgccact
     2401 gctgacccag cttctcagat tgcccttatt gaagacctca tcgctaaaaa agttgatgcc
     2461 attatcgtcg taccgaatga tccggttgca ttagagccag tatttaaaaa agctaatgat
     2521 gccggaatcc ttacttttac ccacgaagcc tccaaccaaa aacaggtaac ctttgatatt
     2581 gaagccttcg ataacgatgc ctatggaaga catatgatgg atgttatggc agaagatttg
     2641 aatttagaag gagaatatgc ttgttttgtt ggccacctta cttcaaccac ccataatgaa
     2701 tgggttgatg ctgaaatcgc ccaacaacag gaaaaatatc cgaacatgaa attggtaacc
     2761 gatcggatcg aagaacagga aaaccaacaa attgcctatg aaaaaacctt ggaattgtta
     2821 aggacctatc caaatcttaa aggtgttatg ggaagcgcca tgagcactgt acctggagca
     2881 gcattagcca tcgaagaaaa aggtctcatt ggcaaagtcg gagcctatgg aacctgcctc
     2941 ccctcggttg cctatgacta cctgaagaac ggagctgcca agtcaatcca tttctggatt
     3001 ccagccgatg ctgggtatgt ctcctgcaag gtagcttggg aagttctcaa agggaatgaa
     3061 atcaaagaag gcatggattt agggaaacct ggttatgaaa gtatcactat tcgaaataat
     3121 caacatggct tcccagtgat ttatggccag gcctggcatg acgttactgc cgaaaacgtt
     3181 gatgattacg acttataatc aataattatt aatagaaatt gccttttctt ttatgtgtcc
     3241 ttaaaaaaga ccttgtcaaa ccatagtttc aaaaatgaac acgataaaat aagtgagttg
     3301 ggtaataaga actatcttcc cagctcactt attttagaag ggataccatg gctgaagatt
     3361 ttttaagaat tcaaaatgta agtaagttat atgccggagt acaagcctta gataatgtca
     3421 gtatgtcaat cggtcagggc gaagtacatt gtttggtcgg ggaaaatggt tctggcaaat
     3481 ctactctcat taaaattata gctggagtgg ttaagccaga tggtggcgaa atagttatcc
     3541 aggggaaaag ttataaaaat cttagaccaa tcgattcaat caatgaaggg attcaagtta
     3601 tatatcagga tttatccttg tttcccaacc tttcggtagc cgaaaacatt tctcttaatc
     3661 agctgattga agaaaaaaag aaaattatta attggaaaga agtcaagagt attgcttcga
     3721 atgttttatt gaccataaaa aaagaacttg atctaaagga aaaggttgaa aatatttcta
     3781 ttgccaataa acagctggta gccatctgcc gtgccctgct acgaaatgcc aaactcatca
     3841 tcatggacga accgactaca gctatcacta agaaagaaat tgataaactc ctatcagtca
     3901 ttttagattt gaaagaaagt ggaatttcta cactatttgt gagtcacaag ttgagtgaag
     3961 ttttgcaaat tgctgaaaaa gtgacggttt taagagatgg gaaaaaagtt ggtgattata
     4021 acgctggtga attagattac gattcccttt ctttttatat gagtgggaaa aaaatcgatc
     4081 attcggtttt cgactatcaa gaaggagcag atcaaaaaac cctattggaa ataaaagacc
     4141 tttctaagaa aagtcaattt gaagatatta gctttaagct aaggccaggt gaaataatcg
     4201 ggatgattgg atcgctgggt tcaggaagaa ctgaagtagc gttgtcctta tttggcttga
     4261 ataaacctga ttctggatca atatatatga atggagagcc agtaaaaatt aactctcccc
     4321 ggaaggcaat cagtttgggt ttatgttatt tgccagaaga ccggcttaat caaggtttgt
     4381 ttttaaaaca gtcgatccaa aataatgtag tcgtcaccaa tttgaaaaaa ctattgacta
     4441 aatctaaact tctcaattac agcaagaaat atgaatatgc caaaacctgg atcgatgaat
     4501 tgaatgtcaa tgttccctct ttagaaatag cagttcaggc gctatccggc ggaaatcagc
     4561 agcgggtagt catagccaag tggttggcca ccaatcccaa ggtatttatc ttagacagcc
     4621 ctaccgttgg aattgatgtc aattctaaaa gtaatatcca taatttgatt cggcagttag
     4681 ctcaaaaagg tattggagtt atcatgattt ccgatgaagt tcccgaaata gtgc
//
LOCUS       DAOSOL010000083         4708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18218_length_4708_cov_3.645605, whole genome shotgun sequence.
ACCESSION   DAOSOL010000083 DAOSOL010000000
VERSION     DAOSOL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18218_length_4708_cov_3.645605"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PK171_03025"
     CDS             <1..764
                     /locus_tag="PK171_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05208.1"
                     /translation="KSCQMPDPVDPARFTGLKERAKELYEGTQYAIIGGSAASLFYLS
                     SELMGFQEYMERLALEPQVIEILVDRILEWEIKFFDKYLEQVGEYVELVWMGDDWGMQ
                     SGPIMNPKIFKKLFMPRYKEFTHFVKSKTQAKIALHSCGSILWAMEDLYEAGVDVIHP
                     LQATAFDMGDPEKIKKSFGNKLVFYSNLSNQSIIPHGTPEDVIKEVRDKIKILAPGGG
                     YIISGGHNIQADVPPQNILALFDTAYQEGHYPIHN"
     gene            874..2016
                     /locus_tag="PK171_03030"
     CDS             874..2016
                     /locus_tag="PK171_03030"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05209.1"
                     /translation="MKARERLITTFQGNKADRVPVSPFIFLNNIYEMFQYKPDIDHFL
                     SPQDFDVAEKFVAYHDYFGFDVLFSPGLLWDSYIPETSDNWEVTITKEGDADSQLRTT
                     LVKTPDGNLTQVMRFNRSSTYMVVLAVEKYIIENKQDFEIFAKYAPPAQFIDYELISK
                     ARKVVNDKGLINVATHGAFNTLNQFRKLEDMMMDPVVDEGFYRAMMDFFLDWNINHLK
                     EVIAAGSDSIEIGGNLATSGVGPRFFQEYVMEYENRLCRQIHEAGAFVVYHNCGDAQK
                     IMHLYNDLDIDVWGYVTTPPFGDVNLDDALRIIRPNMALRGNIDQVEFLRQASPQEIK
                     EKVKWLLDKVKPRGNWILCTSDFFFDGTPYENIQAFAEAGLEYGKY"
     gene            2108..3214
                     /locus_tag="PK171_03035"
     CDS             2108..3214
                     /locus_tag="PK171_03035"
                     /inference="COORDINATES: protein motif:HMM:NF025839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HOT05210.1"
                     /translation="MEKPVYNNFYHYNRNYGCRVQEIIWNGFKTLIIENEKLRVSILV
                     DKGTDIFELLYKPRDIDFMWRSPLEVNTLNPNLPTKSFDTGNYMDTYEGGWQEIIPNI
                     STPSNYKGAAMGFHGELVLLPWKYEIITDTPYEVKVLLKVRMKRAPLLVKKYITLKSN
                     ESLLEFEEIIQNEADEEYQFMWGHHPTYGIPFLDESCVIDLPQGVIGQVYQEDFSGNS
                     IFPANKEFNWPNLKDLRGNQVDLSRVMAPEMKTAFNIYIKNLKDGWYGITNLSKGIGI
                     GFQWDVNIFKYLLLWSVYRGFYGFPFYGKTYNLALELYSAIPDDLDEVIRLNRALCLK
                     PGEELRTIFHTIVYQSSSRIQGFNQQHQPILLDE"
     gene            3249..3324
                     /locus_tag="PK171_03040"
     tRNA            3249..3324
                     /locus_tag="PK171_03040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3282..3284,aa:Thr,seq:cgt)
     gene            complement(3347..3763)
                     /locus_tag="PK171_03045"
     CDS             complement(3347..3763)
                     /locus_tag="PK171_03045"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT05211.1"
                     /translation="MPLDVFINFNGNCREVVKFYAQVFGLEEPAFMTYGEAPPNSQIP
                     VTEETISLVMHASLTIFGTVVMFMDTTPDTQVNQGDNIVLTIRSKDLDEVQSLFNKMK
                     NGGRVLMDLQETFYSKCYGMLIDKFGIPWQFIQDTD"
     gene            complement(3861..4250)
                     /locus_tag="PK171_03050"
     CDS             complement(3861..4250)
                     /locus_tag="PK171_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOT05212.1"
                     /translation="MKSSLIAPCGMNCNLCIGYLREKNRCLGCRENDENILNSCRKCI
                     IKNCTILQQKKLKYCSSKCEKFPCTRLKNLDKRYRTKYQMSMLENLQFIEEFGIRKFV
                     VHEKERWVKEDSIFCVHHKKYYKMEKP"
     gene            4494..>4708
                     /locus_tag="PK171_03055"
     CDS             4494..>4708
                     /locus_tag="PK171_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05213.1"
                     /translation="MLFVILSNSKNGTCKERVARRIQWKNPEGTNLIAEYWLTTEYPN
                     VIQIVETDNVQLLLNAIAEWDDVFDIS"
BASE COUNT         1439 a          882 c          929 g         1458 t
ORIGIN      
        1 ttaaaagctg ccaaatgcct gatccggttg atcctgctcg ttttactggt ttgaaagaaa
       61 gagcgaaaga gctttatgaa ggaacccaat atgccattat tggcggtagc gccgcttcgc
      121 ttttttatct ttcttccgaa ttgatgggtt ttcaggaata catggaacgt ttggctctcg
      181 aacctcaagt tatagaaatc ctggtggatc gcattttaga atgggaaata aagttttttg
      241 ataagtacct ggaacaagtt ggtgaatatg tcgaattggt ttggatgggc gacgattggg
      301 gaatgcagtc aggtccaata atgaacccaa aaatatttaa aaaattattt atgcctcgtt
      361 ataaagaatt tactcatttt gtaaaatcca aaacccaagc aaaaattgct ttgcactcct
      421 gcggctcgat cctttgggca atggaagatt tgtatgaggc tggagtcgat gtcattcatc
      481 ctttgcaagc gactgccttt gatatggggg atccggaaaa aatcaaaaag agttttggca
      541 ataaattggt tttttattcc aacctttcca atcaatccat cattccccat ggtactcccg
      601 aagatgtgat aaaagaagtc cgggataaga tcaaaatttt agctccaggc ggggggtata
      661 tcatttccgg gggtcacaat attcaggcag acgttccacc acaaaacatt cttgccctat
      721 ttgatactgc ctatcaggaa ggtcattatc caattcataa ttgagttaag ggcaattcaa
      781 accaatgaaa agatgaactg ataataattc taaactttta gtcatgaaaa cttgttttaa
      841 attggaaaaa atgaataggg aggagataaa ccaatgaagg cacgagaaag actcattacc
      901 acctttcagg gaaataaagc tgatcgagtg ccggtttcac cgtttatatt cttaaataac
      961 atttatgaaa tgtttcaata taaaccggat attgaccact ttctcagtcc tcaggatttt
     1021 gatgttgctg aaaaatttgt tgcctatcat gactactttg gttttgatgt gcttttttca
     1081 cccggactgt tatgggactc atatattccc gaaacatcgg acaattggga ggttaccata
     1141 actaaggagg gtgatgccga ctcccaactc cgaaccaccc tggttaagac tcctgatgga
     1201 aatttaaccc aggtcatgcg cttcaatcgg agttcaacct acatggtcgt attggctgtt
     1261 gaaaaatata tcattgagaa taagcaggat tttgaaatat ttgccaaata tgctccccca
     1321 gctcagttta tcgattacga gttaatcagt aaagctcgaa aagtagttaa tgataaaggc
     1381 ctcataaacg ttgccaccca tggtgctttt aacactctga atcaattccg aaaattagaa
     1441 gatatgatga tggatccggt ggtggatgag ggattttacc gtgccatgat ggattttttc
     1501 ctagactgga atataaatca tttaaaggaa gtgattgcag ctggttccga ttcaatagaa
     1561 atcggtggaa atttagctac cagcggagtg gggcctcgtt ttttccaaga atatgtcatg
     1621 gaatacgaaa atcgactttg ccgacagatt catgaagccg gagctttcgt ggtatatcac
     1681 aactgtggag atgctcaaaa aatcatgcat ttatataatg acctggatat cgatgtttgg
     1741 ggatatgtaa caactcctcc atttggagat gtcaatcttg atgatgccct gcggattatt
     1801 cgtcccaata tggctcttcg gggaaatatc gatcaggttg aattcctccg tcaggcatca
     1861 ccccaggaaa ttaaagaaaa agtcaaatgg ttgctcgaca aagtaaaacc acgtggaaac
     1921 tggattctct gtaccagcga ctttttcttc gatggaacac cctatgagaa tatccaggct
     1981 tttgctgaag ccggtctcga atatggaaaa tattgatcga attcaatcaa ctaaccataa
     2041 agaaaaggtg aaatgcttta caggctttga atagactata aacttgtgac gagtggggtt
     2101 taagtaaatg gagaaaccag tctataacaa tttttatcac tacaaccgta attacggttg
     2161 tcgggtacag gaaataattt ggaatggttt taaaaccctt atcattgaaa atgaaaaatt
     2221 gcgagttagt attttagtcg ataaagggac ggatattttt gagctgcttt ataagcccag
     2281 ggatattgat tttatgtggc ggagccctct tgaagtaaac accttaaatc caaacttacc
     2341 aaccaagagt tttgatacgg gaaattatat ggatacttat gaaggcggct ggcaggaaat
     2401 catcccgaat atcagcaccc cgagcaatta caagggagca gcgatgggtt ttcatgggga
     2461 attggtttta ttgccctgga aatatgagat tattaccgat acaccctatg aggtgaaagt
     2521 tttattgaaa gtcagaatga agagggctcc tttattagta aaaaagtata tcactctcaa
     2581 atccaatgaa tcccttctgg agttcgaaga gatcattcag aacgaagccg atgaggaata
     2641 ccaattcatg tgggggcatc acccaaccta tgggattccc ttcctcgatg aaagctgtgt
     2701 cattgatctc ccccagggtg tgattggtca ggtttatcag gaagattttt ccggaaatag
     2761 catttttccg gctaataaag aattcaattg gcctaacttg aaagacttgc gaggaaatca
     2821 ggtggacctc agccgagtca tggccccgga aatgaagacg gcttttaata tttatattaa
     2881 aaacctgaaa gacggttggt acgggattac taatttatcc aaagggattg gcattggttt
     2941 tcaatgggat gtcaatattt ttaaatacct gttgttgtgg tcggtctatc gcggttttta
     3001 tggattccct ttttatggaa aaacctataa cctggctttg gagctctatt ctgctattcc
     3061 cgatgattta gatgaggtca tccggctcaa tcgtgcactg tgcctgaagc cgggtgaaga
     3121 actacgcacc atttttcata ccatcgttta tcaatcttct tcccgaatcc aaggttttaa
     3181 ccaacagcac caaccgattc ttcttgacga ataaatgcag ctattgtagt attttgattt
     3241 gcatggatgc cggtgtagct cagcaggcag agcagttgat tcgtaatcaa caggtcacca
     3301 gttcgaatct ggtcgccggc tccagaaggc tcatttataa taacctctaa tccgtatctt
     3361 gtatgaattg ccagggaata ccaaatttat caataagcat cccatagcat ttactataaa
     3421 aggtttcttg taaatccatc aggactcttc cgccattttt catcttattg aacaacgatt
     3481 gaacttcatc taaatcttta cttctaatcg taaggacaat gttatctccc tggttgacct
     3541 gcgtatcagg agttgtatcc ataaacatta caacagtgcc aaaaattgtg aggctggcat
     3601 gcatgactaa gcttatggtt tcttcggtca ccggtatttg ggaattaggc ggagcctctc
     3661 cgtaggtcat gaaggctggt tcttctaaac caaaaacctg agcataaaat ttcactacct
     3721 cacggcagtt tccgttaaaa ttaataaaga catccaaagg catatttttt cctcctcaat
     3781 tttcgaaaat tttgtatttg aatctggtcc tcgttttttc attcctccaa aaaaaagatc
     3841 aataatctct ggaatgaagt tcatggtttt tccatcttat aatatttttt atgatgaaca
     3901 caaaaaatac tgtcttcctt cacccatctt tctttttcat gtactacaaa ttttcgaatt
     3961 ccaaattctt caataaattg taaattttct aacatgctca tttggtattt ggttcggtat
     4021 cttttatcta aatttttcaa tctggtgcag gggaattttt cacatttgct cgagcagtat
     4081 tttaacttct tctgttgaag aatcgtacaa ttttttatga tgcattttct gcaggaattt
     4141 aagatgtttt catcattttc ccgacaacca agacatctgt ttttttctct caaataacca
     4201 atacacagat tgcaattcat tccacaagga gcaattaaac ttgacttcat agaagatcct
     4261 ctttttttaa aaattatatt aataataacc tttggtttta aaattggaaa gccgctcttg
     4321 agaggttttc caatgagcaa taaattaatt accatatcca aattctttat aaatcaaaaa
     4381 ccgagtgata taattttttt actcaataag attaccacaa agcaactcac tccattccta
     4441 cgacattatc ttccttaaaa accggaacaa attttatcaa ggagggtaaa gaaatgttat
     4501 ttgtcatttt gagcaattca aaaaatggga cctgtaaaga gagggtagct cgtcgaatac
     4561 agtggaaaaa tccagaaggg actaacttga tagctgaata ttggttgacg actgaatatc
     4621 ccaatgttat ccaaatcgtt gaaactgata atgtccagct attattaaat gctattgctg
     4681 aatgggatga cgtatttgat atcagcat
//
LOCUS       DAOSOL010000084         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18320_length_4686_cov_3.761823, whole genome shotgun sequence.
ACCESSION   DAOSOL010000084 DAOSOL010000000
VERSION     DAOSOL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18320_length_4686_cov_3.761823"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..508)
                     /locus_tag="PK171_03060"
                     /pseudo
     CDS             complement(1..508)
                     /locus_tag="PK171_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
     gene            complement(1155..1694)
                     /locus_tag="PK171_03065"
     CDS             complement(1155..1694)
                     /locus_tag="PK171_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOT05214.1"
                     /translation="MLFEQKVFTGNWSSLVWRGIIAIIIGLMILVWPAISVVAFLRLI
                     SLVAIIGGIMAVIQAIRTKGGWPLILEGIMSIIIGIIVFSMPGMSALVITLLIGFWML
                     FIGIFQIINVIQFYQVLPNTGKWLIILNGIVSILFGIIVMSRPLLGVLLIVSYVGVYA
                     LLFGVISLFSGFYIHSLSK"
     gene            complement(1778..2479)
                     /locus_tag="PK171_03070"
     CDS             complement(1778..2479)
                     /locus_tag="PK171_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOT05215.1"
                     /translation="MEFYVGTSGWYYSWNKEKNFTWYVNNSQLNAVELNASFYRFPFP
                     NQVKSWAQKGRGIRFSIKVSRRITHIHRLNQESYPVFEAFIRLFKPLESLIDFYFFQF
                     PPSFTPKQFDTLVHFFSFLPHSFQGRIAVEMRNPEWFQKKWIKELEQIPVVFVSVDSP
                     DFQNQIILSNQIIYIRFHGRTGWYNHNYLPHELEEVANQCCSLKPDKIYAFFNNNHFM
                     LDNAQYFFQTLKATV"
     gene            complement(2507..4024)
                     /gene="xylB"
                     /locus_tag="PK171_03075"
     CDS             complement(2507..4024)
                     /gene="xylB"
                     /locus_tag="PK171_03075"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT05216.1"
                     /translation="MAKLILAHDLGTTGNKATLFDENGCLIASDFFGYETYFPDSLSV
                     EQNPEDYWQAVTISTRRLIEKSGFDSDEIAVVSFSGQQMGALPIGADGKPLHRILIWA
                     DRRGVKEVNWVKERIDEDQIYQITGHRLSPNYSMAKILWLKNNFPDVYKNTKYFLMAK
                     DYIVYRLTGSFASDYSDASGTNLFDLVKESWSDEILDAVQIDPAKLPPLYSSSDVVGA
                     ITPEAARDTGLVPGTPVVIGGGDGPCAAAGAGVVREGQAYNYLGSSSWIALASKSPFY
                     DQDKRNFTFHHLSKGLFMPTGTMQAAGGSYQWCRDAICEREKALTRELGLSPYDLMNL
                     EASKIPAGAENLLFLPYLLGERAPWWNPHARGAFIGLTARHRKPHMIRAVLEGVSFNL
                     RIILDAFREQGVEIENMRVIGGGARGAFWAQVLSSMFNMEILRPKHLEEATSLGAAIA
                     GGVGVGIFPGFEIAERLIEIHDCYHPFPEDVKTYDRLYPVFIQAYQSLVQVFECL"
     gene            complement(3999..>4686)
                     /locus_tag="PK171_03080"
     CDS             complement(3999..>4686)
                     /locus_tag="PK171_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT05217.1"
                     /translation="EVMTPRTDMVCIEDNSTLKEFLDLSMNSHFSRIPVFRDDLDNIL
                     GFVHIKDVLPSLEKLGNDDIKVRQFMRPVHFIPATKKIIELLRELQQKRIHMAIVLDE
                     YGGTAGLVTIEDLLEELVGEIRDEHDQEAPPYRQVSENEYLVDASTPIVDLNELLYLQ
                     IPESEEFESIGGLVMEYLGKIPELGEIVDIDHYQIKVERMKGKRIVTLRLMVKNLKEE
                     EDHGETDLSS"
BASE COUNT         1508 a          994 c          823 g         1361 t
ORIGIN      
        1 ttttaattac tgattgaatc ctactatccg tgaaaagggt ttccttgatt ttctgcgatt
       61 caaccaatgc tggagcatga agggagtgac ttatgccgcc tagttttctt tgtaattctc
      121 ttattaattc attcacctga aaatactgct cagcttcacc caaacctcca atcatcggaa
      181 ttcaagaagg ttgcaaaaag gtgagattct catcagaaaa ttcattgata aaggcataaa
      241 tactcttacc ccaccctatt ccaatttttt tattcggtcc aatataattc ataaaaactc
      301 gtagtgctct tttggctaaa tttttataca aaagagggct ttcaggatcg ctatttggaa
      361 caatttctaa aattttaagc ggaaataaag aaatcagttt tttactcagg tcttggtagg
      421 tcgattctga agggtcaata actcgaattt gaacaatgcc caattcatga ccttcttgta
      481 taagccgcac aatctttgtc cgcgacattt gcagcaattc ggcaatttct tgttgagtca
      541 ttttttgttc ataatacatt ctgcaaatct gaataatgag ggattgatta cccaaatcca
      601 tacttcaaca ccctttatta aactgaattt agtttcagaa ccttcaaaaa ctttatataa
      661 aattatacat ttgttcttaa catttgtcta tattggacaa atgttcacta ttctgggggt
      721 caggtcttga ttcttgaatt ttaacctgta atggcattga aaaattgaga aataggcagc
      781 taaaaaagaa gaatgaatga ggtatccacg acttcagaat atgaagtctc aagatgacgc
      841 tagaattcct tctcctttga tgagagaagg cgaggatgag gatggcagac cgggatgaga
      901 tcctcacgcc ctcgaaaagc gagggctcag gatgacggat taaagaattc atttgatggt
      961 attttcagga aagaccctat accatttttt tttaaccaaa cgaaaatgaa aaatattttt
     1021 tcaagaatcg ataatcccag ctcctctttt ttttagcaaa gagagaggga aaaagaggag
     1081 aaattctctc ccaccagggg agaggcaaaa gaagtaaagc actctccccc accgaaagaa
     1141 aggatgcacc tcccctattt actcagacta tggatgtaaa atccgctaaa taaggagatg
     1201 actccaaaca acaaagcata aactcccaca taagatacaa taagaaggac tcccagcaga
     1261 ggacgagaca tgacaataat accaaataaa attgaaacga taccattgag aataattaac
     1321 cattttccag tattaggaag cacttgataa aactgaataa cattgataat ttgaaaaata
     1381 ccaataaata acatccaaaa tccaatgaga agcgtaataa ccaatgctga catcccaggc
     1441 attgagaaga ctataatacc aattataata ctcatgatcc cttccagaat gagcggccat
     1501 cctcctttgg ttcttatcgc ttggatgacc gccattattc caccaataat cgctactaaa
     1561 ctaattaaac gaagaaaagc caccacgcta attgctggcc aaactaaaat catcaaacca
     1621 attattatcg ctataatccc tctccaaacc aaagaactcc agttaccagt aaatactttt
     1681 tgttcaaata gcatcactat aacctccttg actttcctct tttgatagaa tgaactcatt
     1741 aaatatgata tcggaatttt tattaaaaaa tttaatatta aacagtagct tttaatgttt
     1801 gaaaaaaata ttgagcattg tcgagcatga agtggttgtt attaaaaaaa gcatagattt
     1861 tatccggttt taaagaacaa cattggttgg caacttcttc caattcatga ggaagatagt
     1921 tatggttgta ccatccagtt cttccgtgaa aacgaatgta gataatctgg ttagagagaa
     1981 taatttgatt ctgaaaatca ggactgtcca cgcttacaaa aacgactgga atttgctcca
     2041 gctctttgat ccattttttt tgaaaccact caggatttct catttcgact gctattcgac
     2101 cctgaaatga gtgaggaaga aatgaaaaaa aatgaactaa agtatcaaat tgttttgggg
     2161 tgaacgaggg aggaaactgg aaaaagtaaa aatcaataag actttcaaga ggtttaaaaa
     2221 gccggataaa ggcctcaaat actggataag attcttgatt taaacgatga atatgagtaa
     2281 ttcgacggga aaccttaatg gaaaaacgaa tacctctccc tttttgtgcc caggatttta
     2341 cttggtttgg aaaagggaaa cgataaaaac tggcattgag ttcaactgcg tttaattgac
     2401 tattatttac ataccaggta aaattttttt ccttattcca agaataatac catccgctgg
     2461 tcccaacata gaactccaag taaaaaaatc ccctcctgac tttttcctat aaacactcaa
     2521 acacctgtac caaggattga taagcttgaa taaaaaccgg gtaaagccgg tcgtaggttt
     2581 tgacgtcttc gggaaaaggg tgataacaat cgtggatttc tattaatctt tcggcaattt
     2641 caaacccagg aaaaatgcct accccaactc ctcccgcgat tgctgcacct aaagaagtcg
     2701 cttcttcaag atgcttcggt cgcaatatct ccatattaaa catactggat aaaacctgag
     2761 cccaaaatgc tcctcgagct cctccaccaa taactcgcat attttcaatc tctacacctt
     2821 gttctcggaa agcatcaagg attattcgaa gattgaaact cactccctca aggactgccc
     2881 tgatcatatg gggttttcgg tgacgtgcgg ttaaaccgat aaatgcacct cgagcatggg
     2941 ggttccacca gggggcacgt tccccaagaa gatagggtag gaacagaaga ttttcggctc
     3001 cggccggaat tttgcttgct tctaaattca ttaaatcata aggactaaga ccaagctcac
     3061 gagtaagggc tttttctctt tcacaaatgg cgtcccgaca ccactgataa gaaccacccg
     3121 ccgcctgcat ggtaccagtt ggcataaata agcctttgga tagatgatgg aaagtaaagt
     3181 tccttttatc ctgatcataa aaagggctct tgcttgccaa ggcaatccag gaagaagaac
     3241 ccaagtaatt ataggcttga ccctctctca ccactccagc acccgccgct gcacagggac
     3301 catccccgcc accaatgact actggtgtac caggaactaa accggtgtct ctggctgctt
     3361 ctggtgttat cgctcctacc acatccgagg aagaatagag tggtgggagt ttagctggat
     3421 cgatttgaac agcgtctaaa atttcatcag accaggactc ctttaccaaa tcaaacaaat
     3481 tggtaccaga agcatccgag taatcactgg caaaacttcc cgttaaccga tatacaatat
     3541 aatcctttgc cataagaaaa tacttggtat ttttatagac atctggaaag ttatttttta
     3601 accagaggat tttcgccatg gaataattcg gacttaatcg gtgtccggta atttgataaa
     3661 tttggtcttc atcaattctt tctttaaccc aattgacctc tttgactcct cgtcgatcag
     3721 cccaaattaa aattctatgc aggggcttcc cgtcggcacc aatcggcaaa gcacccatct
     3781 gttgtccgga aaaactcact accgctatct catcggaatc aaaaccactt ttttcaataa
     3841 gtcttcgagt cgagatggtt actgcttgcc aataatcttc cggattttgt tcaactgata
     3901 gagaatcagg gaaatacgtt tcataaccaa aaaaatcact ggcaatcaaa caaccatttt
     3961 catcaaacaa agtcgcctta ttcccggttg ttcctaggtc atgagctaag atcagtttcg
     4021 ccatggtctt cttcctcctt aagatttttt accattaaac gcagagtgac gatccttttt
     4081 cctttcattc tttcaacctt aatctgataa tggtcaatat caactatttc acccaattcc
     4141 ggtatttttc ctaaatactc catgaccaag ccaccgattg attcaaattc ctcgctttcg
     4201 gggatttgga gataaagaag ttcgtttaag tcaacaatcg gtgtcgaagc atccactaaa
     4261 tattcgtttt cgctaacctg acgataaggc ggggcttctt gatcatgttc atcgcgaatt
     4321 tcaccaacca gttcttcaag aagatcctcg atggttacca gtccagctgt ccccccatat
     4381 tcatctaaaa cgattgccat atggattctt ttttgctgta gctctctcaa gagttcgata
     4441 atctttttgg tagctgggat aaaatgaacc ggtcgcataa actgtcgaac ttttatatca
     4501 tcatttccta atttttctaa ggaaggaaga acatctttaa tatgaacaaa tcctaaaata
     4561 ttatctaaat catctcgaaa aaccggtatt cttgagaaat gagaattcat tgataaatca
     4621 agaaattcct ttagcgtcga gttgtcttcg atgcatacca tatcggtccg aggagtcatg
     4681 acttca
//
LOCUS       DAOSOL010000085         4686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18322_length_4686_cov_3.468581, whole genome shotgun sequence.
ACCESSION   DAOSOL010000085 DAOSOL010000000
VERSION     DAOSOL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18322_length_4686_cov_3.468581"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PK171_03085"
     CDS             <1..907
                     /locus_tag="PK171_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05218.1"
                     /translation="LSIPENVIYGIAGESGCGKTTLLKALFASIEPPLQLFGGKVSYL
                     IKNNFIDALSLNGKEKRELRWSYISYIPQGSMSVFNPVKKLKVTYLDFLESHVRGKAG
                     RELLSLARQHLEQLGLSPKVLDAFPHQLSGGMRQRIAIALSALLKPQVILADEPTTAL
                     DVVAQRAVLQLMRDIQQEIKNTIVLVTHDMGIHANITTCMAIMYAGKIVEVGPTQEIF
                     SNPLHPYTKFLIHSLPRIGDKMPKKSVPGAPPSLLNPPSGCPFHPRCPEISDFCQDKV
                     PDLNNSTQNHKVACWLRRESCDCNS"
     gene            891..1844
                     /locus_tag="PK171_03090"
     CDS             891..1844
                     /locus_tag="PK171_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05219.1"
                     /translation="MTAILKARNLTKIYSLGGLFSRTKITAVDRVSFSVQPAEIFTLA
                     GESGCGKTTTARMILGFEYPTAGELLYENQNLVELRDKKAWFKKVQAVFQNPFETFNP
                     LRKVEDYFFETIQNFRLDKSMKKAEELIEEKLQAVGLSLCDIAGKYPGELSGGQLQRA
                     SIARSLLTNPSLLIADEPVSMVDASLRMSVVNLFRKMSDSFRVSVIYITHDLATAYYI
                     SDRIAIMFRGNIVEIGPVDQVLENPKHPYTKLLIESIPQPDPQIPEKVPIKILEEKEE
                     YLRKGCKFSGRCPEVQNICKQQIPEDISLNGTVVKCHLYQK"
     gene            1893..4166
                     /locus_tag="PK171_03095"
     CDS             1893..4166
                     /locus_tag="PK171_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOT05220.1"
                     /translation="MKKGIHMIENLLSKMTLEEKIAQLGSVHACQLVENGSFSSKKAN
                     DLLRHGIGQITRLIGDPDAEPQTAAEIGNAIQRFLKKETRLQIPAIIHEECLSGLTGR
                     GATVFPQAIGLASTFCPELVKKMTAVIRKQIRSMGGHQGLAPVLDIPRDPRWGRTEET
                     FGEDPYLVSRLGVAYIQGLQGEDLQTGVMATAKHFTAYGISEGGRNMGPARAGERELR
                     EVFLFPFEVAVKEAQVGSVMNAYHHIDGIPCTASQFLLTKVLREEWGFSGIVVSDYEA
                     IKMLETLHHVVQNSKEAAQKALQAGIDIELPDTICYGEPLLETVWEGLISEELINEVV
                     RRVLTAKLHLGLFEDEIYINPVLVPSLLDPEENRQLALEIAQASIILLKNDGVLPLKK
                     DLKTLAIIGPNAREGLHLHGDYSYSVHIPSVQAWKGKKAVWQDSVNTVNIFEGIKNKL
                     PQGEVLYAKGCDLSGNSQEGFEEALEIAKKAQVIVAVMGENSGLFQQSISGEGSDRSD
                     LELPGMQGKLLRELSRLNKPIILVLVNGRPLALQWEKENIPAIIETWYPGEEGGNAIA
                     DVLFGDYNPGGKLPFSFPKELGQLPVYYNRVSLAFNQYISTDAQALFPFGHGLSYTRF
                     EYSDLNISPKEIKSLEKIAIQCKVKNIGHQAGDEVIQLYIQDPIASLVRPVKELKGFK
                     RIHLEPDEERTIVFTLFPDQLAFYDEHMRLIIEPGLFYVMVGSSSEDIRLSGQFEAIK
                     EFQLTKYRHFKSEVIVQ"
     gene            complement(4483..>4686)
                     /locus_tag="PK171_03100"
     CDS             complement(4483..>4686)
                     /locus_tag="PK171_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05221.1"
                     /translation="CCFKEKKLEFCGDCPDYKQCKIIHDFYNKNGYKYKKYRQSLEFI
                     QNKGYDEFLRLAERWKGAYGKLI"
BASE COUNT         1413 a          962 c          924 g         1387 t
ORIGIN      
        1 cctaagtatc cccgaaaacg taatctatgg tattgccgga gaaagcggtt gcgggaaaac
       61 tacacttctc aaagctttat ttgcttctat tgagcctcct cttcagttat ttggaggaaa
      121 ggtaagttac ttgattaaaa acaattttat cgatgcactc agccttaacg ggaaggagaa
      181 acgagagtta cgctggtctt atatctctta tatcccccaa ggatcgatga gtgtttttaa
      241 tccggttaaa aagctgaagg taacctattt ggattttctg gaaagtcacg ttcgaggaaa
      301 agccggtcgc gaacttcttt ctttagctcg ccaacatctt gaacaattag ggctttcacc
      361 taaggtttta gatgcttttc cccatcaatt atcaggagga atgcgacaaa gaatcgctat
      421 tgctctctcc gccttgctga agccccaggt aatacttgcc gatgaaccaa ccacggctct
      481 tgatgtcgta gctcaaagag cagttctcca acttatgcgg gatatccaac aagaaatcaa
      541 aaatactatt gttttagtga ctcacgatat gggtattcac gctaatatca ctacctgtat
      601 ggctattatg tatgccggta aaattgttga ggtaggaccg actcaagaaa tcttttccaa
      661 tcctcttcat ccctatacta aatttcttat acattctctt cctcggattg gagataaaat
      721 gcccaaaaaa agtgtgcctg gcgcccctcc ttctctcctc aatcccccat cgggatgtcc
      781 ttttcatccg agatgtcctg aaatttctga cttttgtcaa gataaagtac ccgaccttaa
      841 taacagcacc caaaaccata aagttgcttg ctggctaagg agggaatcat gtgactgcaa
      901 ttcttaaagc acgaaatcta accaaaatat attccttagg aggattgttc tctcgaacga
      961 aaattaccgc ggtggatcga gtttcatttt ccgttcaacc agctgaaatc ttcactctgg
     1021 ctggagaaag tggctgcggg aaaacgacga ctgcccgaat gattcttgga tttgaatacc
     1081 cgaccgctgg cgagctactt tatgaaaatc aaaatctggt tgagctgaga gataaaaaag
     1141 cctggtttaa aaaggttcaa gctgttttcc aaaatccctt tgaaactttt aatccactac
     1201 gcaaagttga agattatttt tttgaaacca tccaaaactt ccgtttagac aagagcatga
     1261 aaaaggctga ggagctaatt gaggaaaaac ttcaagctgt tggtttatct ctttgcgata
     1321 tcgccggaaa atatcccggt gaactttctg ggggacaact gcaacgagca tctatcgccc
     1381 gatctctttt aaccaatccc tcacttctta tcgctgatga gccagtatcg atggttgatg
     1441 cctctctgcg aatgtcggta gtaaatcttt ttcgaaaaat gagcgattct tttcgggtga
     1501 gtgtaatcta tattactcat gacttggcta ctgcttacta tatcagtgat cggatagcca
     1561 tcatgtttag aggaaatatt gttgaaatcg gtccggttga ccaagtgttg gagaatccga
     1621 aacatcctta caccaaactc cttatcgaat ctatccctca accggatcct caaataccag
     1681 aaaaagttcc cattaaaatt ttggaagaaa aagaagaata ccttcgcaag gggtgtaaat
     1741 tttccggccg ttgtccggaa gttcaaaata tttgcaaaca acaaattccc gaagatatct
     1801 ctttaaatgg tactgtagtg aaatgtcatc tctaccaaaa atagttttgc ttggaattaa
     1861 tcttaaaaat atcaatttgg agtgattatc ctatgaaaaa aggaattcat atgattgaaa
     1921 atcttctatc aaaaatgact ttggaagaaa agattgccca gttaggttcg gttcatgctt
     1981 gtcaattggt ggaaaacggc tcattttctt ctaagaaagc caatgatctc ctgcgtcatg
     2041 gaattggcca aattacccgc ctgataggcg atccagatgc cgaaccccaa actgctgcag
     2101 aaataggaaa cgcaatccaa cgttttctta aaaaagaaac tcgtttacag attccagcca
     2161 ttatccatga ggagtgcttg agcggactta ccggaagagg ggctacagta ttcccccagg
     2221 caattggttt agccagtact ttttgtcctg agttggttaa aaaaatgacc gccgttatcc
     2281 gaaaacaaat ccgtagtatg ggaggccatc agggtttagc accagtcctg gatattcctc
     2341 gagatcctcg ctggggtaga accgaggaaa cttttggtga agacccttat ttagtttcgc
     2401 gattaggtgt tgcctatatt caagggcttc aaggagagga ccttcaaacc ggagttatgg
     2461 ccaccgccaa acatttcact gcttacggca tttctgaggg aggaagaaat atgggacctg
     2521 cccgggcagg agaaagagaa ttaagagaag tctttctctt cccctttgag gttgcagtaa
     2581 aagaagctca ggttggttcg gttatgaatg cttatcatca tatcgatgga attccctgca
     2641 ctgcttccca attccttctt accaaggtcc tccgtgaaga atggggattc tctggaatag
     2701 ttgtatccga ttacgaagca attaaaatgc ttgaaactct ccatcatgtc gttcaaaact
     2761 ccaaagaagc tgcccagaaa gctctgcaag ctggtattga tattgagctt cctgatacga
     2821 tttgttatgg tgagcctctt cttgaaacag tttgggaagg attaattagc gaagagctga
     2881 ttaacgaggt agtccgaaga gtattgaccg ccaaactgca tttaggtctt tttgaggatg
     2941 agatatatat taaccctgtt ctggtgccat ctcttttgga tcctgaagaa aatcgtcaat
     3001 tagctctgga aatagctcaa gcttctataa tccttctgaa aaacgatggt gtactccctc
     3061 ttaaaaaaga tcttaagact ttagctatca ttggtccaaa tgcccgggaa ggtcttcacc
     3121 tccatggaga ttacagctat tcagtccaca ttcccagcgt gcaagcctgg aaaggtaaaa
     3181 aggcagtttg gcaggattcg gtcaatactg tgaatatctt cgaaggtatt aaaaataaac
     3241 tcccccaagg agaagtactt tacgctaaag gatgtgatct cagcggaaat tctcaagagg
     3301 gcttcgaaga agctcttgaa attgccaaaa aagctcaagt gattgttgca gttatgggtg
     3361 agaatagcgg tctctttcaa caatccatat ccggtgaagg cagcgatcgt tcagacttag
     3421 aacttccggg tatgcaggga aagcttttaa gggaattaag tcgtttgaat aagcctatta
     3481 ttttggtact tgttaatggc cgacctcttg cgcttcaatg ggaaaaagaa aatattcctg
     3541 ccattattga aacttggtat ccaggtgaag aaggaggcaa cgcaatagct gacgttttgt
     3601 ttggcgacta taatcccgga ggaaagcttc ctttttcctt ccctaaagaa ttgggacaac
     3661 tccctgtgta ttacaatcga gtttccttag cttttaacca gtacatttct accgatgctc
     3721 aagccctctt cccctttgga catggtttaa gttatacccg ttttgaatat tccgacctga
     3781 atatttctcc aaaagaaatt aaatcgctgg aaaaaattgc aattcaatgt aaagtgaaaa
     3841 atatcggcca tcaagctggt gatgaagtca tacagctcta tatccaagac ccgatcgctt
     3901 ccctggttcg tccggttaaa gagttgaaag gattcaagag aatacatctc gaacctgacg
     3961 aagaacgaac cattgtattt actctttttc ctgatcaatt agccttttat gatgagcata
     4021 tgcggttaat tattgaacca ggtcttttct atgtaatggt tggttcatca tcagaggata
     4081 tccgactttc aggtcaattt gaagcaatca aagaatttca attaacgaaa taccgacatt
     4141 tcaaaagtga agttattgtt caataaagaa aattgtataa taatgtaatt cattaaattg
     4201 tcattctttt cttatgaatt gaataatgat tgaatagtga tcataatatg cccataccaa
     4261 agatttaaaa agaatagggg cgcaataaat tgcgcccttt tcaaaaagat gggattctca
     4321 agacgaataa tacgctcccc aaaatgaccg agagggtgaa tagccaaagt cattatcctt
     4381 ttttcttgat tgatggaatg tttgacaacc ttgattcggc aaaaatccat ccactcctaa
     4441 taatctcaaa aaattaactc aagccaaagc tctcaataat ttctatatta atttcccgta
     4501 agcgcctttc catctctcag ctaatcttaa aaactcatcg tatcccttat tttgaataaa
     4561 ttctaatgat tgtctatact ttttgtactt atatccattt ttattataga aatcatgaat
     4621 tattttgcac tgtttataat ctggacaatc accacagaat tctaatttct tttctttgaa
     4681 acaaca
//
LOCUS       DAOSOL010000086         4666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18425_length_4666_cov_3.585990, whole genome shotgun sequence.
ACCESSION   DAOSOL010000086 DAOSOL010000000
VERSION     DAOSOL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18425_length_4666_cov_3.585990"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1169)
                     /gene="hemW"
                     /locus_tag="PK171_03105"
     CDS             complement(21..1169)
                     /gene="hemW"
                     /locus_tag="PK171_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT05222.1"
                     /translation="MREIALYIHVPFCIQKCRYCDFVSYPYLQPESISDYFKVLTQEL
                     QIRTENDKLQNVVLKSIYFGGGTPSLVSPEILNDFLLLLRQNFRFSESIEITVEANPG
                     TLNSEKIREFAHLGVNRLSIGIQSFQNRFLSFLGRAYREKEVHNLLIDLIDTGLFLNW
                     SFDLMYGLPFQTFSDWKKEMEHLLMYQPPHFSLYNLTLSHQVPLRIFLKKHFALFPGQ
                     DWEASIYRWTQQILRKYGYRRYEISNFAKPGYECLHNLTYWRNQEYLGLGVSSSSYLG
                     GIRMRNASTLSHYQKRIKNCQSPVVTKDILNLKQKLAEEMILALRTSEGVETEKLFKT
                     YPESLVQEKLIQLEDFAQSHFIHEREGRWILTSRGTLIANQLFLEILD"
     gene            complement(1166..2974)
                     /gene="lepA"
                     /locus_tag="PK171_03110"
     CDS             complement(1166..2974)
                     /gene="lepA"
                     /locus_tag="PK171_03110"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT05223.1"
                     /translation="MTENQSIRNFCIIAHIDHGKSTLADRILDLCHTIPKQKMRDQVL
                     DRMDLERERGITIKAKAVRLQYFSSQRNSSYVFNMIDTPGHADFSYEVSRSLAACEGA
                     LLVIDATQGVEAQTLAHTNIALTQGLAILPVINKIDLPSANPARVYEEIESILGPDHL
                     PITLVSAKLGEGVEELLEKVIDYIPSPQGQREKSLKALVFDSVYDSYRGVLPFIRIFE
                     GLIHKGFEIMMYSNKQKYQVIEIGIFNPDMKEIDSLGPGEVGYISCNIKAIQDCRVGD
                     TITSAANPTPQAVPGYKQAKPMVFCSFYPVNAEGYEILKEALEKLQLNDASLMYEPDI
                     SEALGFGFRCGFLGLLHMEIIQERIEREFDIEVVATAPHVVYHVLTKNEEIIEVKSPR
                     DFPPAGEIEEAEEPIVEMKIIAPSTAIGGVMDLCQSKRGVLQDMTYLDPSTVIIRYHL
                     PMAEILFDFYNRLKSVSRGYASMDYEFFGYASSNLVKVDILVSKEKVDGLSFISHEEN
                     AARRARELVTRLRKIIPRQMFTVTIQAAIGGKIIAREDVAALRKDVLAKCYGGDITRK
                     RKLLEKQKEGKKRMKAVGKISLPQEAFLAVLQTEEQ"
     gene            complement(2979..4541)
                     /gene="murJ"
                     /locus_tag="PK171_03115"
     CDS             complement(2979..4541)
                     /gene="murJ"
                     /locus_tag="PK171_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOT05224.1"
                     /translation="MTKERSLIRNTAVVGAGTFLSRITGLIREILVSSMFGASWVTDV
                     FFVAYTIPNLLRRLFAEGALSTSVIPVFSQYSEESHDESQKFIYSIFTGLTLVVSVVC
                     VIGIAFSPWIVEMVGVGFRSEPDKIHMAINMTRIMFPFLLIISWSALVMGILNTLHIF
                     SWSAIAPVFFNAGTILTLLLLRAYAGPYSLAIGVLIGGIGQFVIQLYPFYKKGFVLKF
                     FRKFWQDPGFREVIRLMLPVTLALAVGQINTLVDRIIASTCQEGAVSALYYADRLLEL
                     PLGIFGIALSTAILPSLSQLVFKNNPQEWNNTFSQGIRLVLFIMIPITTYLAIFRNEA
                     IRLIYERGMFDTVASQMTAQSLLFYLPGLVFFSMNHIITKAFYSLKDTVTPVRISVIM
                     VGVNVVLDLILVRYLSFSGLALATSLVAVLNCICLFISLKRKHAFYEKDHTFCYWLVK
                     IIILNLLFIGLCWGILHVVQNLQSILSLLAFFGISVVVFSLIYILLSQSIGLKEGKSI
                     WSFILRKLVGKG"
BASE COUNT         1513 a          965 c          843 g         1345 t
ORIGIN      
        1 agctgccttt ttgtgttccc ttaatctaaa atctcaagga ataattggtt ggcaattaaa
       61 gtcccccggg aagtaagaat ccacctccct tccctctcat gaataaaatg actctgagca
      121 aaatcttcta actgaatgag cttctcttga actaaagact ccggatacgt tttgaatagt
      181 ttttccgttt caactccctc gctggttcgt aaagcgagaa tcatctcttc cgctaatttt
      241 tgtttaaggt ttagaatgtc tttggttacc actggagatt gacaattttt gattcttttt
      301 tgataatgac tcaacgtcga ggcatttctc atcctgattc ctcctaaata agaactggac
      361 gataccccca aacccaaata ttcttgattt cgccaatagg taagattgtg aaggcattca
      421 tacccaggtt tggcaaaatt tgatatttca tatcttctat atccatactt tcgtaatatt
      481 tgctgtgtcc accggtaaat cgatgcttcc cagtcctggc caggaaacaa ggcgaagtgt
      541 ttttttaaaa aaattctcag cggtacctgg tggctcaggg tgagattgta gagagaaaaa
      601 tgaggcggtt gatacattaa tagatgttcc atttcttttt tccaatcaga aaaggtttga
      661 aaaggaagac catacatcaa atcgaaactc cagttcaaaa acaaaccggt atcgattaaa
      721 tctatcaaaa ggttatgaac ttctttttcc cgataagctc gtcctaaaaa agacaaaaag
      781 cggttctgaa aactttgaat acctatactg agacggttaa ctcctaaatg agcaaactcg
      841 cgaatttttt ctgaattcaa cgttccagga ttcgcttcta cggttatttc gatcgattct
      901 gaaaaccgaa agttttgacg aaggaggagt aaaaaatcat ttaaaatttc cggtgaaacc
      961 agagatgggg tccccccacc aaaataaata ctttttaaaa cgacattttg taacttatcg
     1021 ttctcggtac gtatttgcag ctcttgagta agaaccttaa aataatcaga aatagattcg
     1081 ggctgaagat agggatagga aacaaaatca caataacggc acttttgaat acaaaaggga
     1141 acatgaatat ataaagcaat ttctctcatt gctcttcagt ttgtaaaacg gctaaaaatg
     1201 cttcctgagg gaggcttatt tttcctaccg ccttcatcct ctttttccct tctttttgct
     1261 tttctaaaag ttttcttttc cgggtaatgt ctccaccata acatttagct aatacatctt
     1321 ttcttagagc agcaacatct tctcgagcaa tgatttttcc cccaatggca gcttgaattg
     1381 taacggtaaa catttggcga gggattattt tcctaagacg ggtcactaat tctcgtgccc
     1441 gacgggctgc attttcttcg tggctaataa aggataatcc atcaaccttt tctttgctca
     1501 ctaaaatatc cactttaact aaattggatg aggcgtaacc aaaaaattca tagtccatac
     1561 ttgcataccc acgacttact gatttaagcc ggttataaaa atcgaaaaga atttcagcca
     1621 tggggaggtg gtaacgtata atcaccgtac ttggatccaa gtaagtcatg tcttgcaaaa
     1681 cacctcgctt ggattggcag aggtccataa ctccaccaat ggcagtcgat ggagctataa
     1741 ttttcatttc aacaattggt tcctcggctt cttcaatttc tcctgcagga gggaaatccc
     1801 gaggggattt tacttcaata atttcctcat ttttggtcaa gacgtgataa accacatgag
     1861 gagcagttgc taccacttca atgtcaaact ctcgttcgat acgttcttga atgatttcca
     1921 tatggagtag acctaaaaat ccacagcgaa agccgaatcc caaagcttca gaaatatctg
     1981 gttcgtacat caaagacgca tcgttgagtt ggagtttttc caatgcctct tttaagatct
     2041 cgtacccttc ggcattcact ggataaaaac tacaaaaaac catcggtttt gcctgtttat
     2101 aacccggtac ggcttgagga gtcggattgg ccgccgaagt tatggtgtct ccgacccgac
     2161 aatcttgaat agcctttata ttacaagaaa tatatccaac ctcaccaggc ccgagagaat
     2221 caatctcttt catgtcagga ttaaaaatac ctatttctat tacttggtat ttctgtttat
     2281 ttgaatacat catgatttca aaacctttat gaatgagtcc ctcaaaaatt cgtataaaag
     2341 gcaaaacccc ccgataggaa tcatataccg aatcaaatac caaggccttt aaggattttt
     2401 ctctctggcc ttgaggggat ggaatatagt caattacttt ttctaagagt tcttcaactc
     2461 cctctcccaa tttggcactc accaaagtaa taggaagatg gtctggacca agaatactct
     2521 caatttcttc ataaactcga gcgggattag ctgaaggaag atctatttta ttgattaccg
     2581 gcaatatagc caaaccttgg gttaaagcta tattggtatg agctaaagtt tgtgcctcaa
     2641 caccttgagt ggcatcaatt accaataatg ctccttcaca agccgctaaa cttcttgaca
     2701 cttcataact gaaatcggca tgaccggggg tatcgatcat attaaaaaca taagaggaat
     2761 tccgctgaga agaaaaatac tgcaatctta ccgcttttgc cttgatagtt attcctcttt
     2821 ctcgctccaa atccatacga tccaatactt gatctcgcat tttttgttta ggaatagtat
     2881 ggcataaatc caatatccga tcagccaagg ttgattttcc gtggtcgata tgagcgatta
     2941 tacaaaaatt acgaatcgat tgattctctg tcataatatt aaccctttcc tactagctta
     3001 cgtaaaataa aagaccatat agattttcct tctttcaatc ctatagattg agacagtaag
     3061 atatatatca agctaaaaac gactaccgaa atcccgaaaa aggccagcag cgatagtata
     3121 ctctgtaagt tttgcacaac atgaaggata ccccagcaaa gcccgataaa cagaagattt
     3181 aaaattatta tcttaaccaa ccaatagcaa aaggtatggt ctttttcata aaaagcatgt
     3241 tttctcttga gagaaataaa taaacagata caattgagta ctgccaccag agaggttgcc
     3301 aaggctaaac cactaaaact taaataacga accaatatta aatctaaaac tacatttaca
     3361 cctaccataa taacactgat tcgaactggt gtcacagtat ccttaagcga atagaaggct
     3421 ttggtgataa tgtgattcat cgagaaaaaa accagtccgg gaaggtaaaa caaaagactc
     3481 tgggcagtca tctgagatgc aaccgtgtca aacattcctc gttcatatat taagcgtatg
     3541 gcttcatttc gaaatatagc caggtaagtt gtaatgggaa tcattatgaa taaaaccaat
     3601 cggattcctt gagaaaaagt attattccat tcttgaggat tatttttaaa tactaactgt
     3661 gacagggaag gaagaatggc ggttgaaaga gcaataccaa aaattcctaa ggggagttct
     3721 aataaacgat cagcataata gagtgcggat accgctcctt cttgacaagt tgaagcaatg
     3781 attcgatcga ccaaggtgtt tatttgacca actgccagag ccaaggtaac tggcagcatg
     3841 agtcgaatta cctcacgaaa acctgggtct tgccaaaatt ttctgaaaaa tttgagaaca
     3901 aatccttttt tataaaaggg ataaagctga ataacaaatt gaccaatacc accaatcagc
     3961 acaccaattg ctaaagaata cgggccggca taggctctga gcaacaaaag agtaagaatt
     4021 gtcccggcat taaaaaatac cggcgctatc gctgaccaac tgaaaatgtg gagggtgttc
     4081 agtattccca tgaccagagc cgaccaggat attatgagca aaaaggggaa cataatacgg
     4141 gtcatgttga tagccatatg aattttatcc ggttcggaac gaaagccaac tccaaccatc
     4201 tcaacgatcc aaggtgaaaa agctattcct ataacgcaaa caactgatac taccaaggta
     4261 agaccggtaa atattgaata aataaacttt tgggattcat cgtggctttc ttccgaatat
     4321 tgagaaaata ccggaataac tgaagtactg agtgccccct ctgcaaacaa gcgacgaagt
     4381 aggtttggta tggtgtaggc aacaaaaaat acatcagtta cccaagaagc tccaaacatc
     4441 gaggaaacca ggatttccct gatgagtccg gtaatacggg aaagaaatgt accagctcca
     4501 actaccgcgg tatttctgat gagtgatcgt tctttagtca tactctcatt atatacgaat
     4561 catattattc ttgttaactc ataaccaaag taacaatgat gatatattca aaaaaatagg
     4621 gctgaagatc atctctccag ccctattctc aagtactctc atatta
//
LOCUS       DAOSOL010000087         4607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18752_length_4607_cov_3.975395, whole genome shotgun sequence.
ACCESSION   DAOSOL010000087 DAOSOL010000000
VERSION     DAOSOL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18752_length_4607_cov_3.975395"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PK171_03120"
     CDS             complement(<1..1257)
                     /locus_tag="PK171_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3769 domain-containing protein"
                     /protein_id="HOT05225.1"
                     /translation="MNQLHLFFIKAMKKPVLVAFFLLLTIPLTTSIGAKEGIRINADT
                     LLYDDAGSQVEAIGNVHLSWKDISFATNQMLFRFDTSEAFIPGFIKARFGNYQISAEK
                     MYYNFQTRNGWFESAELVYELSEGGNLYFRGSKIEYQKGKWTGTDLLVTGCPHSPPLY
                     SVRAKEVLIYPQDRILISGLGFFIKEKRIIQLPAYSRLLNNHGSNFIPSLGYQRKKGL
                     FVEGNYEYLFSENLLFQANLYYSTLQSSRISSDITLQYPYVEARIFIDLWQNEESTMG
                     GYAHYQKNGLSLWMLGIENERFNDEVISRLPQFIASYRTESENGFFIEANLSYGKFSQ
                     GDMSTWRNDVYLSAGLEKKNYGGEIFYQNINLSVLDDATVWGGKVWAEKEFSSHFSAG
                     IFYEYTQVSGETYFFFDPKDTNIFGLE"
     gene            complement(1254..2036)
                     /locus_tag="PK171_03125"
     CDS             complement(1254..2036)
                     /locus_tag="PK171_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05226.1"
                     /translation="MNKKTLILLFLIPCIVVLFISFTSLAKTDADKIYSQTQKKWTQS
                     QTVGDAFSVKAVFWNPELVQAWVAKYGAENLLSLEEQTAYHRDFIQRERFQRYLVFDV
                     TIEKLTGPALFPLNFTKNTYLTDDQGNKYYLLEFPREFDDKIFDKVSGKMYFSRIGKN
                     DQPIVSPDTKKITLHFSHLSIEPSYVAQNVELIWKDPYIPPDYTQVSWQPELEEEILR
                     LQERIILLEAEKKELIENQKLIESEIENVKNKIEELQANLKQ"
     gene            complement(2041..3222)
                     /locus_tag="PK171_03130"
     CDS             complement(2041..3222)
                     /locus_tag="PK171_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05227.1"
                     /translation="MKMASVLCISMLILALVFPAYGQDQVASPLVKDLENMERILYGV
                     PETGSVLIRTENLERDLIGDTLSGTLMERLNTLKTFILTGTPEEPSLDFKLKSIRLSL
                     NTAEQKTGILTAQLDEFEQLIFGVPSAEPIGVRVDKLYRTVVNTSYTSNFQVTVPVSS
                     LVKVSIQTNLNSEKNNVGDPVPYVLIEDFIVNDILIAPAGTEGEGWISKIRRKGNFGR
                     AGMIQIDFGSITSLDGTPIVLTLGDKAIQENKAFAYAIGTSIVGLVALGPIGAVAGFL
                     INGEPAKVESGSELFVETTQELVVSGPIASSVSYQTYQDQGYTTTEIDTYQPSYNQDQ
                     NIPEVEIEVKPVETWDAEEDIEETVDVTSIATPIPIQQSKPSPTSGIDIEIKPYQEGD
                     F"
     gene            complement(3328..4290)
                     /locus_tag="PK171_03135"
     CDS             complement(3328..4290)
                     /locus_tag="PK171_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05228.1"
                     /translation="MNAVLEVKKLKKYFSTTKGVVKAVNDVSFSITPGETLGLVGESG
                     SGKSTVAYTVMGLYQPSGGQILFQGQDVAISHHKRKRILKKELQIVFQDPGSSLNPRQ
                     TIENILALPIRLHDRDYKKGIAEKVQEILSLVELPVEYMEKYPRSLGGGEKQMVAIAR
                     ALATNPSLIVLDEPTSALDVSIQAKIIHKLIEIQQEKNLGYLFITHDLSLMRNIAHRV
                     AIMYLGRIVETAPAQQFFETPYHPYTRMLISSIPVVSSEEEKLKPGKIPSMGEIPSPV
                     NMPAGCVFHPRCVNRMPICDQEEPIDFEIKPGHWVKCHLWSPNK"
     gene            complement(4287..>4607)
                     /locus_tag="PK171_03140"
     CDS             complement(4287..>4607)
                     /locus_tag="PK171_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT05229.1"
                     /translation="IREWTNRVYVMYAGSIVEESTTEELFSHPLHPYTRSLMDCVPKL
                     TGGGTAEGIPGRIPDYFNPPTGCRFHPRCPHAFALCSEKKPALYHVNDTHQVACYLYQ
                     EVNQ"
BASE COUNT         1364 a          880 c          896 g         1467 t
ORIGIN      
        1 ctccaaaccg aaaatattgg tatctttggg atcaaagaaa aaataggttt ctccacttac
       61 ttgagtatat tcataaaata ttcctgcgct aaaatgagaa gaaaattctt tttccgccca
      121 gacctttcct ccccaaacag ttgcatcatc aagaacggaa aggttaatgt tttgatagaa
      181 aatttctccc ccgtagttct ttttttccaa tcctgcgctt aaataaacat cgtttcgcca
      241 agtactcata tcaccctggg aaaattttcc ataacttaaa ttcgcttcaa taaaaaatcc
      301 attttcagat tcagtacggt aagaagcgat gaattggggt aaacgactga tgacttcatc
      361 attaaacctt tcgttttcaa tacctaacat ccagagagat aatccatttt tttgataatg
      421 agcataaccc cccatggtag actcttcatt ttgccaaaga tcaataaata tccgtgcttc
      481 aacataggga tactgaaggg ttatgtcact acttattcga ctgctttgta aggtggaata
      541 gtatagatta gcctgaaata acaagttttc cgagaaaaga tattcatagt tgccttcaac
      601 aaagagcccc ttttttcttt ggtaacccaa gctcggtata aaattagaac catgattgtt
      661 gaggagccgg gaataggcag gtagttgaat aattcttttt tctttgatga aaaaccctaa
      721 tccgctgatt aatatccgat cctgtggata gattaaaact tccttagcac gaacgctgta
      781 taagggaggt gaatgtggac atccggtaac caataggtcg gtaccggtcc attttccttt
      841 ttgatactcg atcttcgaac ctcgaaagta aagatttcct ccttcgctta attcatatac
      901 caattctgcg ctttcaaacc aaccatttct ggtttgaaag ttgtagtaca ttttttctgc
      961 actaatttga taattaccaa agcgagcctt tataaagcct ggaataaagg cttcagatgt
     1021 atcaaaccga aaaagcatct gattggttgc aaaagaaata tccttccagg ataggtggac
     1081 atttccaatg gcttcaacct gcgaaccggc atcgtcataa aggagagtat cggcattaat
     1141 tcgaatgcct tcttttgctc cgatgcttgt cgttaaagga atagttaata atagaaaaaa
     1201 agcgaccaat actggttttt tcatcgcttt tataaaaaag agatgaagtt ggttcattgc
     1261 tttaggtttg cttgcagctc ctcgatttta ttttttacat tttcaatttc agattcaatc
     1321 agtttttgat tttcaatcaa ctcttttttt tctgcttcca gcagaataat tctttcttgc
     1381 aaacggagga tttcttcttc aagttctggt tgccaactta cttgggtgta atcaggagga
     1441 atataaggat ctttccaaat gagttcgaca ttttgagcga cataagaagg ttcgattgac
     1501 aaatgagaaa aatgaagagt aatttttttg gtatctgggc taactattgg ttgatcattc
     1561 ttcccgattc gactgaaata catttttcct gatactttgt caaaaatttt atcatcaaat
     1621 tcacgaggga actcaagtag gtaatactta ttgccttgat cgtcggttaa ataagtgttt
     1681 ttagtaaaat tcaaagggaa aagagcgggt cccgtgagtt tttcaatggt tacatcaaaa
     1741 actaaatatc gttgaaaacg ctctctttgt atgaagtcac gatgataagc agtttgctct
     1801 tccaaagaaa gaagattttc agctccgtat ttggcgaccc aggcctgcac caattctgga
     1861 ttccaaaata cagcttttac cgagaaagca tccccaacgg tttgactttg ggtccatttt
     1921 ttttgggttt gagaataaat tttgtcggca tcggtttttg ctaaggatgt aaagctaata
     1981 aataaaacaa caatacaagg aatgaggaac aaaaggatga gggttttttt attcatattt
     2041 ttaaaaatct ccttcctggt agggtttaat ttcgatatca atacccgatg ttggtgatgg
     2101 tttggattgt tgaattggaa ttggtgtggc aatgctggtg acatcaactg tttcttcgat
     2161 atcttcttca gcatcccaag tctcaactgg cttaacttca atttctacct ccgggatatt
     2221 ttgatcttga ttataggagg gttggtaagt gtcaatttcg gttgtggtat aaccttgatc
     2281 ttgataggtt tgatatgaaa ctgacgaagc aattgggccg gatacaacca attcctgagt
     2341 ggtttccaca aatagctcac tacctgattc aactttggct ggttctccat tgattaaaaa
     2401 tccagctacg gctccgatcg gaccaagtgc gaccaggcca acaatactgg ttccaattgc
     2461 ataagcaaat gctttatttt cttgaatagc tttatctccc aaagtaagaa ctattggggt
     2521 accatcaaga ctggtgattg acccaaaatc aatttgaatc ataccagctc ttccgaaatt
     2581 tcctttgcgg cggattttgc tgatccagcc ttctccttcg gttccagctg gagctataag
     2641 aatatcattt acaataaaat cttcgattaa aacataagga actgggtcgc caacattatt
     2701 tttttctgaa tttaaattgg tttgaatact aacttttact aaagaagaaa caggaacggt
     2761 aacctggaaa tttgatgtgt aggaagtatt tactacggtt cggtaaagct tatcaacccg
     2821 gacaccgata ggttcggcgc tgggaacacc aaatatgagc tgttcaaatt cgtccagttg
     2881 agcggttaaa attccagttt tctgctcggc agtattgaga gaaagtcgaa ttgacttgag
     2941 tttaaaatct aatgaaggtt cttcaggtgt accggttaaa atgaaggttt tcagagtatt
     3001 gagcctttcc attaaagttc ctgacagggt atcaccaatt aaatctctct caaggttttc
     3061 agtacggatt aaaacacttc cagtttctgg aacaccataa agtatacgtt ccatattttc
     3121 caagtctttt actaaaggtg atgctacctg gtcttggcca taggctggaa aaacaagcgc
     3181 cagtatcaac atacttatac acaaaacgga agccatcttc atagcactca ctccttcata
     3241 tttcttctta tcattatacc gtagagttgt tccatctaaa gggttatgag taaaaaattt
     3301 ttcaatgatt ctatgaatag aaatcaatta tttattagga gaccagagat ggcattttac
     3361 ccaatgacct ggtttgatct caaaatcgat tggttcctct tgatcacaaa tcggcatccg
     3421 attaacacaa cgaggatgaa atacacaacc tgcgggcata tttactggac tagggatttc
     3481 ccccattgaa gggatttttc ctggttttaa tttttcttct tctgaggaga caaccggaat
     3541 cgaggatatc agcattcggg tataaggatg ataaggagtt tcaaaaaact gttgagccgg
     3601 tgctgtttca actatacgac ccaggtacat gatagcgaca cggtgggcaa tatttctcat
     3661 taagcttaaa tcatgggtaa tgaataggta tcccaagttt ttttcttgtt ggatttcaat
     3721 gagtttatgg ataatttttg cctgaattga tacatccaga gctgaggttg gttcatctaa
     3781 aacaatgagt gaaggattag ttgcaagagc cctagcaata gccaccatct gtttttcacc
     3841 acccccaaga gaacgaggat atttttccat atactcgacg ggaagttcta ctaaggacaa
     3901 tatttcttgc actttttcag caatcccttt tttataatcc ctatcgtgaa gacgaatggg
     3961 cagggctaag atgttttcta tggtttgtct tggattaagc gaagatcccg ggtcctggaa
     4021 aacgatttgt agttcttttt ttaatattcg ttttcttttg tggtgagaga tagcaacatc
     4081 ttgcccttgg aaaagaatct gaccaccact tggttgatag agtcccataa cggtataagc
     4141 aaccgtactc tttcccgaac ccgattctcc aactagtccg agagtttcac ccggtgtaat
     4201 agaaaaactg acatcattga ctgcttttac aactcctttg gtagtcgaga aatatttttt
     4261 aagttttttt acttcaagta ctgcgttcac tggtttacct cctggtagag ataacatgct
     4321 acctgatgag tgtcgttgac atgatatagg gcgggttttt tttcagagca caaagcaaaa
     4381 gcatgaggac aacgaggatg aaaacgacaa ccagtaggcg gattaaaata atcgggaatt
     4441 cttccgggaa tcccctcggc ggttcctccg ccggttaatt tggggacaca atccattaat
     4501 gatcgagtat aaggatggag gggatgggaa aataactctt cagttgttga ttcttcaacg
     4561 attgatccgg catacataac ataaacccga ttggtccatt cacgaat
//
LOCUS       DAOSOL010000088         4604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18773_length_4604_cov_3.687184, whole genome shotgun sequence.
ACCESSION   DAOSOL010000088 DAOSOL010000000
VERSION     DAOSOL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18773_length_4604_cov_3.687184"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PK171_03145"
     CDS             <1..872
                     /locus_tag="PK171_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05230.1"
                     /translation="LLNDPENKKAFQEKYGKELKVPETWDEFKEIALFLSKPPEIYGT
                     QFAGKEEALSGRFWDILMSHGGKIIDENYKPAFNSPEGIKVATMLRDLYQAGAMPPGM
                     VNFLWDDVAGTFANGNIAMYTEWYGWYSYFQNPEASKVAGKFDISRQPKGDAGIHGGW
                     AGAHCFSVTKSSKNKEAAVAFIKFITSYEPQLFEAQIGYIPVRNSVFETLIQEAEKST
                     DPLIKKRFEIMQIQINEDFTPPPIVAEWIPISNVLYPKLQAIMLGDVEVEEGLNQAAK
                     EVELIMEEAGYYD"
     gene            934..1863
                     /locus_tag="PK171_03150"
     CDS             934..1863
                     /locus_tag="PK171_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05231.1"
                     /translation="MNQEMNLRLKKDLKKSSKNDRYLSWFMIIPALAVVAVLIVVPMI
                     YSFSLSLTNMNLLKPASTQFIGFRNYIRLFQDEIFWRAFWNTILFMTLAVNVEFILGL
                     FIAQLMFKVTRGQGVIRTAIMAPMMFAPVLVGFQFKWFFNDQVGLVNNLLYSLTGDYQ
                     IIPWLIHYPMNLLAILIAEIWMSTPFMVIIFLAGLVSLPTEPFEAASVDGASGWQQFR
                     YITLPLISPFIYIAMVIRSLDLGRAYDLVRIMTGGGPANRSEMIWTYTHRLAITNNRF
                     GMGTAMSFITVAVSFAFVIYLFRQLSKSLQEVY"
     gene            1863..2747
                     /locus_tag="PK171_03155"
     CDS             1863..2747
                     /locus_tag="PK171_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05232.1"
                     /translation="MLDRQGINIRPVSKRQRKIKQEFYNLLVYFLVLIFFLPVLWIIM
                     TAMRPEVEVNARPPVYIPSRLSIETFAQLLGASHTEKSIPFYSYLKNSLLTSIFSTFL
                     ALVTGTFAGYSFARFRFKGGNQLFIGMMLARSIPGISVSLPLFVIFARTGLLDRISGL
                     ILVYTAMTIPFTTWLMQGFFKDIPSTLDEAAQIDGCSRWQAFLRIDLPLALPGLAASG
                     IFAFLTSWNEFQIASVITRTPLSKTFPVGLYDFTQEFTIDWRGMCAMSVIMLIPAIFF
                     VLLTQKQLIKGLTFGAIK"
     gene            3145..4266
                     /locus_tag="PK171_03160"
     CDS             3145..4266
                     /locus_tag="PK171_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05233.1"
                     /translation="MKKAEEKIKRVVTALKLREEPDRVPLSDFYWSSFYQNWLQEFDL
                     PPDTDIYKYYDLDLKVISPNMDPKVESCKVIEQNSEYVLFKSGFGCVVKKVFNYPMPM
                     FIDFEVKNVQDFAKYVFEDPRDERRYFEKRCDIINCGDSFGQLNSYMEAVEANWNEFC
                     LFGSICEPYETMWRIRGTEGLLMDLALFPEKVKEFANRCTDFMLGIAERQIELTRPQG
                     MFIWGDVAYDKGMFISPSLWREIFFPCVKRLCDYIHSQGVITVYHGCGKSLAIFEDLI
                     ETGVDVYNPLEAKAGMDPVELKQKYGDRIAFYGGLDTRLLGEGNWEDIEKEVLYKLNA
                     AKGGGYLPASDHSVAGNVNPRWYDRMINLLKQKGTYPIK"
     gene            4372..>4604
                     /locus_tag="PK171_03165"
     CDS             4372..>4604
                     /locus_tag="PK171_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT05234.1"
                     /translation="MSTIYDIAKRAGVSPATVSRALNNQNLVKEETRKKIHKIAEEMN
                     YSPNFLARSLVKKQTNTIALIISDITNPFFTTVA"
BASE COUNT         1344 a          801 c          969 g         1490 t
ORIGIN      
        1 atttgttaaa cgatccggaa aataagaaag catttcaaga aaaatatggc aaagaattaa
       61 aagtccccga aacttgggat gaattcaaag aaattgctct cttcctctcc aaacctcctg
      121 aaatctatgg gactcagttt gccgggaagg aagaggcttt atccggtcgc ttttgggata
      181 ttttaatgtc tcatggcggg aagattatcg atgaaaatta taaacctgct tttaatagcc
      241 cagaaggtat caaagttgca accatgcttc gagatcttta tcaagcggga gccatgccgc
      301 ctggtatggt taactttttg tgggatgatg ttgctggtac atttgccaat ggaaatatcg
      361 ctatgtacac tgaatggtat ggctggtatt cttattttca aaatcccgaa gcttctaagg
      421 tggcagggaa gttcgatatt agtcgtcaac cgaaaggtga tgctggtatc cacggtggtt
      481 gggcaggagc ccattgcttt tcagtaacta aatcaagtaa aaacaaagag gcagcggttg
      541 cctttataaa gtttatcacc agttatgaac cacagttatt cgaagcccag attggatata
      601 ttccagttcg aaactcagtt tttgaaactc ttattcaaga agcagaaaaa tcaaccgatc
      661 cactcattaa gaaacgtttt gagataatgc agattcagat taacgaagac ttcacacctc
      721 ctccgattgt tgctgaatgg attcccatct caaatgtctt atatcccaag ctccaagcca
      781 ttatgttggg tgatgtagaa gttgaagagg gtcttaatca agctgcaaaa gaagttgaat
      841 tgattatgga agaagccggc tactatgact aatttataaa aacggggggg gtaccccccc
      901 gtttttagtt tttaaagggg gtgtccggct ttaatgaatc aggaaatgaa ccttaggtta
      961 aaaaaagatt taaaaaaatc atcaaaaaac gaccgttatt tatcttggtt tatgataatt
     1021 ccagctcttg ccgttgttgc tgtgttaata gttgtcccca tgatatattc cttttcttta
     1081 agtttaacca atatgaattt attaaaacca gcgtcgactc agtttattgg ttttcgaaac
     1141 tatatccgac tttttcagga cgaaatattt tggcgagctt tttggaatac tattctattc
     1201 atgactttag cggttaatgt tgaatttatt ttgggacttt ttattgctca actgatgttt
     1261 aaagtaaccc ggggacaagg agtcattcgt actgctatta tggctcccat gatgtttgcc
     1321 ccagttttgg tcggatttca atttaagtgg ttttttaatg atcaagtagg tttagtcaat
     1381 aatcttttgt attcactgac tggagactat caaattattc cctggctcat tcattatcca
     1441 atgaatctgt tggctattct cattgctgaa atttggatga gcaccccatt tatggttatt
     1501 atttttctag ctggattagt gagcttacca actgagcctt tcgaggcggc ttcggtagat
     1561 ggagcaagtg gttggcagca gttccgctat ataacccttc ctttgataag cccatttatc
     1621 tatattgcca tggttattcg ctccctcgat cttgggaggg cttatgattt ggtccgaatt
     1681 atgaccggag gtggaccagc caatcgatcg gaaatgattt ggacctatac ccatcgttta
     1741 gccataacca acaatagatt tggaatggga actgcgatgt cttttattac ggtagctgtt
     1801 tcgtttgctt tcgttatcta cttattcaga caattatcaa aaagccttca ggaggtctat
     1861 taatgttgga tcgccaagga atcaatattc gtccagtttc aaaaagacaa aggaaaataa
     1921 agcaggaatt ttataatctt ttagtatatt ttctcgtatt gatttttttt cttccggtat
     1981 tatggattat tatgactgct atgcgacctg aagttgaggt gaatgctcgg ccaccggtct
     2041 atattccttc tcgtttaagt attgagactt ttgctcagtt attaggggca agtcatactg
     2101 aaaagtcaat tcccttttat agttatctca aaaactctct cttgacttca atttttagca
     2161 cgtttttagc cttggtaaca ggaacttttg ctgggtatag ttttgctcgt tttcgattta
     2221 agggtgggaa tcaacttttt ataggtatga tgttagcccg ctcaatacca ggtatttcgg
     2281 tgagcttacc tttgtttgta atttttgctc gtacgggttt gttggatcga atttcaggcc
     2341 ttattcttgt ttatacagcc atgaccattc cttttacaac ttggttgatg cagggatttt
     2401 ttaaagatat tccttccacg ctcgatgaag cagcgcagat agatggatgt tctcgttggc
     2461 aagccttcct gcgtattgac cttcctcttg ctcttcctgg actggccgcc agtggaatat
     2521 ttgctttttt aacttcctgg aatgaattcc aaattgccag tgttattacc cgaacaccac
     2581 tctctaaaac ctttccagtt ggcttatatg attttactca ggagtttacc attgattgga
     2641 gaggaatgtg tgccatgtcg gtgattatgc tgatcccagc catctttttt gtattgctta
     2701 cccagaaaca actcatcaaa ggattaactt ttggagcaat taaataatag gtcaaacttt
     2761 ttttgtttat tcgggattgt ttgcgcattc cccctcacct taaacctctc tcattcttct
     2821 tctcttgcgg atgagagagg ttaataacaa ataggtaata aggcgaacta gagagcagac
     2881 aaaataatga agaccaaaaa gatgagacct tcctacaggg aaatcatcac tcaggagaca
     2941 cctttggtgc caggtgaggc ttttgccgtc tcaaaaagtt gagccgttgg ataatattac
     3001 gtcattaagg caccatatct atcgttgagc ttcttgaaag gacgactcaa agttcctact
     3061 cccttgttgg gaaaaggtaa agatgagggc gaaaatttct cattactgcc ttaataggaa
     3121 agaaaatttt aaggaggaca aaaaatgaag aaggctgagg agaaaataaa gagggttgtg
     3181 actgcattaa aattaagaga agaacccgat cgggtcccat tgagtgattt ttactggtcg
     3241 agcttttacc aaaattggct ccaagaattt gatttaccac ctgataccga tatttataaa
     3301 tattacgatt tagatctaaa agtcatctct ccaaatatgg atccgaaggt agagagttgt
     3361 aaagttattg agcaaaactc tgaatacgtt ctttttaaaa gtggttttgg atgtgtggtt
     3421 aaaaaagtat ttaattatcc catgccaatg tttatagatt ttgaagtgaa aaacgttcaa
     3481 gattttgcta agtatgtttt tgaggatccg agagatgaaa gacgttattt tgaaaaacgc
     3541 tgtgacatta tcaattgtgg tgactcgttt ggtcaattga atagttatat ggaagcagtc
     3601 gaagcgaatt ggaatgaatt ctgtttattt ggctccattt gtgagcccta cgaaacgatg
     3661 tggcgtatta ggggaaccga aggattactg atggatcttg ccttgtttcc ggaaaaagtt
     3721 aaagaatttg ccaatcgttg caccgatttt atgttaggaa ttgccgaacg tcaaattgag
     3781 ttgacacgcc ctcaaggaat gtttatatgg ggtgatgttg cttacgataa agggatgttc
     3841 atttcccctt ctctttggag ggaaatattt tttccatgtg ttaaaagatt atgcgattat
     3901 atccattctc aaggggttat aacagtatat catggttgtg ggaaaagcct ggctattttt
     3961 gaagatttga ttgaaaccgg ggttgatgtt tataatcctt tagaagcgaa ggcaggaatg
     4021 gatccagttg agctgaaaca aaaatatgga gacagaattg ccttttatgg tgggttggat
     4081 actcggttac ttggagaagg caattgggag gatattgaga aggaagttct ttataaatta
     4141 aatgcggcca aaggtggagg atatttgccg gcctcagatc attcagtagc aggaaacgtg
     4201 aacccccgat ggtatgatcg aatgattaac ttgctcaaac aaaaagggac ttatcccatt
     4261 aaataataat gagcgaagaa caaaaatcag aatgtaaaat tgctataatc cttaaaatta
     4321 aagaatattt caatttgtta aatattctac tggaatgttg tggtgatttc attgtcgact
     4381 atatacgata tagccaaaag agcaggagtt tccccggcaa cggtttctcg ggcgcttaat
     4441 aatcaaaatt tagtgaaaga agaaacccga aaaaagattc ataaaattgc cgaagaaatg
     4501 aattattctc ccaatttttt agccagaagc ctggttaaaa aacaaaccaa cactatagct
     4561 cttatcattt ctgatatcac gaatcctttt tttactactg tggc
//
LOCUS       DAOSOL010000089         4577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_18941_length_4577_cov_4.432773, whole genome shotgun sequence.
ACCESSION   DAOSOL010000089 DAOSOL010000000
VERSION     DAOSOL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18941_length_4577_cov_4.432773"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PK171_03170"
     CDS             complement(<1..799)
                     /locus_tag="PK171_03170"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT05235.1"
                     /translation="MKAAVYYGPGDLRIEESPLPEIGPGDILLKVGACAICGTDMRIF
                     RHGHRGVKIPQIVGHEIAGTVAEIGKDVKGYAVGDKVAVDPIVSCGECFYCRRGLTNL
                     CLTFKENYEAFGYYYPGGFAEYMRIPEKAIRRGNLILIKDDLALEEAAIAEPMACALN
                     GQMLSQVGVGDHVLIIGAGPIGCMHISLAKTLGATKVIISEFQEGRLNLARQFGADIY
                     VNPMQEDLKEVLMKATNGIGPSVIIISAPARKAQEMALDLAANQARIN"
     gene            complement(836..2035)
                     /locus_tag="PK171_03175"
     CDS             complement(836..2035)
                     /locus_tag="PK171_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05236.1"
                     /translation="MNQSVQFGAGNIGRGFIGHLLWESGYRIVFVEAYPDLVKLLNAR
                     SNYPLRLLEKNGQEKNVTIDNLVAYQTEEKNNISQAIAVSSVIFTAVGVKNLPAIAPL
                     VADGIQLRLKENPIPINILLCENLKDAPQFFKNEVKTHLNTEGKQFLQEKVGFVGTVV
                     ARMVPVMDKRFGVDDPLFIVAEAYHKLPYDVTAVKGVFPEISGLKPVQNFPSEVDKKL
                     FIHNLGHATLAYFGYLKGYEYIHQAIADEEIKNLLDEVLNETSTSILRKYPDLDRVEI
                     NEFVEDLKERFFNPLLMDTVYRVGRDPIRKLGEDDRIIGGIALCQSQKVFPAAIIKVT
                     GLALNYDYNKDADAVKLQKMIKDKGIKEVIHKLCNLDPETDIGEKIIQSYYQFRERFR
                     ILKENKL"
     gene            complement(2063..2995)
                     /locus_tag="PK171_03180"
     CDS             complement(2063..2995)
                     /locus_tag="PK171_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOT05237.1"
                     /translation="MAKVTCIGILVADLLGRPINRLPERGKLVLIPQMELHVGGGANN
                     TGVVLKKLGEEVVMVGKVGQDGLGEFIINSLRKEGIDPKGITITQEYSTSATMVLVDE
                     QGERSFIHCAGANQSLRNADIQDEFFHDSDIVHITGTFLMPGFDGPETTALLKRVKEA
                     GVTTSIDTYWDDSGQWLNVIEPYLPLLDIFISNRDESTRISGRQNIVDNAKFFLDYGI
                     QIVAIKMGEEGSFIMTQNEKILVPPFQVKAVDGTGAGDAFAAGFLVGYLKKWDLYETG
                     RFANACGAMCVQAMGATDGVGNFDEVMDFVRKHS"
     gene            complement(2998..4002)
                     /locus_tag="PK171_03185"
     CDS             complement(2998..4002)
                     /locus_tag="PK171_03185"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOT05238.1"
                     /translation="MNDYSYFDFLIKGLAMNLTDENVELMTRIAWLHYMEGLTQNEIG
                     QQLKLSQPKVARLIDKAQKNGIIKISIESPLSNCLKIESTIRKQFGLQDVVVIPTLQE
                     KEIYESIGRAGAWYMEKVLNNGDLVGIAWGRTLKHLALAIRSAKVKNLKFVTMVGGLT
                     SSASLNPYTIGEKLASIFEGECYYLYAPAVVESEEIRNFYLNERINQNTLQLACQAKW
                     SLVGIGTVDVRYSIYSLTGFIDYHELEALKQKGAVGDILGQFYTIDGTILDTPLHRRT
                     VAVPLENIQKMHNVIGVAGGEEKADAILGALRGHFINILITDEKTAMKIMSRISVLEG
                     "
     gene            complement(4112..>4577)
                     /locus_tag="PK171_03190"
     CDS             complement(4112..>4577)
                     /locus_tag="PK171_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOT05239.1"
                     /translation="PIKNAIHAFKYRKILSLRILFIHLLVEFLSSNPFFRDIDGILPV
                     PLHQSRLREREFNQAEILARGISEVLQKPLISKAVTRKKKTLPQVGLSMKERRLNLQG
                     AFRVENEKFLAKKKILIIDDVLTSRSTVDSLSLVLRRAGSQTILVLALATGK"
BASE COUNT         1387 a         1000 c          768 g         1422 t
ORIGIN      
        1 agttgattcg ggcttggtta gcagccagat ccaacgccat ttcttgtgct tttcgagcag
       61 gagcggaaat gataataacc gaaggaccaa ttccattggt cgccttcatc agaacttctt
      121 tcagatcttc ctgcatggga ttcacataaa tatcagctcc gaactgacga gccaagttca
      181 atcgaccctc ctggaattca gaaatgatga ctttcgttgc tccaagggtt tttgctaaag
      241 aaatatgcat gcaacctatt ggtccggctc caataattaa gacatgatca ccaactccaa
      301 cttgggacaa catctgacca tttaaagcgc aagccattgg ttctgcaata gcagcttctt
      361 ccaaagccaa gtcgtcttta atcagaatga gattccctcg ccgaatggct ttttctggaa
      421 tccgcatata ttcagcgaaa cccccgggat aatagtatcc aaaagcttca taattttctt
      481 tgaaagtaag gcacaggttg gttaatcccc gtcggcaata aaaacattca ccgcaggata
      541 caataggatc aactgcaact ttatccccaa cagcataccc cttcacgtct ttcccaattt
      601 cagcgacagt accggcaatc tcatgaccaa caatttgggg aatttttaca ccgcgatgcc
      661 catgacgaaa aatccgcata tcggttccac atattgcaca cgcaccgact ttcagaagta
      721 tatctcccgg tccaatttcc ggaaggggac tttcttcaat tctcaaatcg cccggtccgt
      781 agtaaacagc tgctttcatt tactccactc ctctcaattc ttcccagagg gaagtttaca
      841 atttattttc cttcaatatc cgaaaccgct ctctaaactg ataataggat tggataattt
      901 tttcaccaat atcggtttca ggatcaagat tacacaactt atgaatgact tctttaatcc
      961 ctttgtcctt aatcattttc tggagtttta ccgcatcggc atccttatta taatcatagt
     1021 tcaatgctaa tccggtcacc tttattattg cagctggaaa gactttttga gattgacaaa
     1081 gagcgatccc tccaattatg cggtcatcct caccaagttt gcgaattggg tctctcccaa
     1141 ctcgataaac tgtatccata agtaacggat taaagaaacg ctcctttaga tcttcaacaa
     1201 attcgttaat ttcaactcga tctaaatccg gatacttccg aagaattgaa gttgaagttt
     1261 cattgagcac ttcatcaaga agatttttta tttcttcatc ggcaattgcc tgatggatgt
     1321 attcatatcc cttcaagtaa ccgaaatagg ctagggttgc gtgacctaaa ttatggatga
     1381 agagtttttt gtcaacctca gaaggaaaat tttgaactgg tttcaaaccc gagatttcag
     1441 gaaaaactcc cttcacagca gtcacatcat aaggaagttt atgataggct tcagcaacaa
     1501 taaacagagg gtcatcaaca ccaaatcgct tatccattac tggaaccata cgggcaacaa
     1561 ccgttccaac aaaaccaact ttttcctgaa gaaattgttt tccttcggta tttaaatgag
     1621 ttttcacttc atttttaaaa aattgaggcg catctttaag attttcacac aaaagaatat
     1681 tgatagggat aggattttct ttgaggcgaa gctgaatccc atcagctact aaaggagcaa
     1741 tggccggcaa attttttaca ccaactgctg taaaaataac cgatgaaaca gcaatagctt
     1801 gtgaaatatt gtttttttct tcggtctgat aagcaactaa attatctatg gtcacatttt
     1861 tttcttgacc gtttttttct aataaccgaa gggggtagtt tgagcgagca ttgagaagtt
     1921 taacaagatc tggataggct tcaacaaaaa caatccgata acccgattcc caaagaagat
     1981 gaccaataaa acctcttcca atgttacctg ctccaaattg aacagactgg ttcattcaaa
     2041 aacctctcat ttcttttaaa atttacgagt gtttacgaac aaaatccatt acttcatcaa
     2101 agttcccaac accatcggta gctcccatag cttgaacgca catggctcca caggcgttcg
     2161 caaatcggcc agtttcatat aaatcccatt tttttaaata gccaactaag aaaccagcag
     2221 caaaagcatc gccagcaccg gttccatcta cagctttcac ttggaaggga ggaactaata
     2281 ttttttcgtt ttgagtcatg ataaaactcc cttcttctcc catcttgata gctacaattt
     2341 gaataccata atcgagaaaa aatttggcat tatccactat attctgacga ccagaaatac
     2401 gggtagactc atcccgatta gaaataaata tatcgaggag aggaaggtag ggctcaatga
     2461 cgttgagcca ttgtccgctg tcatcccaat aagtatcaat tgaagtggta acgccggctt
     2521 ctttaacccg ctttaaaaga gcggttgttt caggcccatc aaaaccaggc attaaaaatg
     2581 tacctgtgat atgaacgata tccgagtcgt gaaaaaattc atcttgaata tcagcatttc
     2641 ttaagctttg attagctcct gcacagtgaa taaaggaacg ctctccctgc tcatcgacta
     2701 aaaccatagt tgccgaggtt gaatattctt gtgttatggt tatgcctttg ggatcaatcc
     2761 cctcttttcg aagtgaattg atgataaatt cacccaaacc atcttgtcca actttcccca
     2821 ccatcaccac ttcttcgcct aattttttca ataccactcc ggtattgtta gctcctccac
     2881 ccacatggag ttccatttgt ggaattaaaa ccaatttccc tctttctggt aatcgattga
     2941 taggccgacc taagagatcg gcaaccagga ttcctatgca agttactttg gccattttta
     3001 tccctccaga actgatattc gactcataat tttcattgcg gtcttttcat cggtgataag
     3061 tatattgata aaatgacccc ggagagctcc aagaatagca tcggcttttt cttcgccacc
     3121 agccaccccg attacattgt gcattttttg aatattttca agtggcactg caactgttct
     3181 tcgatgtaag ggcgtatcta aaatggttcc atcaatggta taaaattgac ccaaaatatc
     3241 tcctactgct cctttttgtt ttaatgcttc taactcatga taatctataa atccagttaa
     3301 agagtaaatt gaataacgaa catcaaccgt accaataccc actaaagacc atttagcctg
     3361 acaggcaagt tgaagagtat tttgatttat tctttcattc aaataaaaat ttcttatttc
     3421 ttcactttca accacggctg gtgcataaag ataatagcac tcaccttcaa atattgaagc
     3481 cagtttttcc ccgatagtat agggattaag cgaagctgag gaagtcaagc ctccaaccat
     3541 ggtaacgaat ttaagattct tgacttttgc cgaacgaatc gctaaggcca ggtgttttag
     3601 ggtccgcccc caggcaattc caaccaaatc tccattattt aaaacctttt ccatatacca
     3661 agcccctgct cgaccaatgc tttcataaat ctctttttcc tgaagggttg ggattactac
     3721 aacatcttgt agcccaaatt gtttacgaat tgtcgattca atttttaaac aattggaaag
     3781 aggtgattca atcgatatct ttataattcc atttttttgc gctttgtcga taagtctggc
     3841 aactttaggt tgagaaagtt ttaattgttg gccaatttca ttttgggtga gaccttccat
     3901 ataatgaagc caagcaattc gagtcatcaa ttcaacattt tcatcggtaa gattcatggc
     3961 taatccttta attaaaaaat caaaatatga ataatcattc atatttttaa tatccattca
     4021 aatctatttt atatttttcc ttaataatgt caatatttta taaaattatg aatattattt
     4081 cagctttaat ttattaaatc atccttattt tttattttcc agttgctaaa gctaaaacta
     4141 atatggtttg acttcctgct cgacgtaata ccaaggaaag actatccaca gtggaacgag
     4201 aggttaaaac atcatcgatg attaatattt ttttctttgc taaaaatttt tcattctcca
     4261 cccgaaaggc gccttggaga ttgagccttc tctctttcat gcttagccct acttgtggta
     4321 gagttttctt tttccgggtt acggctttac tgatgagagg tttttgtaaa acttcagaaa
     4381 tacctcgtgc taagatttcg gcttgattaa actctctttc acgaagacga gattgatgga
     4441 gtggtaccgg cagaattcca tcaatatcac gaaagaaggg atttgaagat aaaaattcga
     4501 ccaataaatg aataaataaa attcgtaacg atagaatctt tcgatactta aaagcatgaa
     4561 tagcattttt aatcggg
//
LOCUS       DAOSOL010000090         4539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19167_length_4539_cov_3.394291, whole genome shotgun sequence.
ACCESSION   DAOSOL010000090 DAOSOL010000000
VERSION     DAOSOL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19167_length_4539_cov_3.394291"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..980)
                     /locus_tag="PK171_03195"
     CDS             complement(213..980)
                     /locus_tag="PK171_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05240.1"
                     /translation="MDDQKIPVLEVKDIVKRFGGLVAVNKVSMEVAPGEVVGLLGDNG
                     AGKSTLIKVVSGVYHQDEGQVIFQGKEVKIGNPMDAIRLGIETIYQDLALAENLNVYS
                     NIFLGREKLKKALGLIDVLDHDYMHQESKKVLDQLGIEIPSLKNKIKMLSGGQRQSVA
                     ISRSIYWNAKLLIMDEPTAALGVAEQRKVLDLVRTLSAQGVGIIIISHQLYDVFQVAH
                     RLVILRRGEKVGELMVKETNPDEVVSLMVGAELVQHE"
     gene            complement(986..2074)
                     /locus_tag="PK171_03200"
     CDS             complement(986..2074)
                     /locus_tag="PK171_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05241.1"
                     /translation="MSAEDIRKLSGNKREWGVLIGRSWVYLFLIGEIIIFSLLGTGFF
                     SAKNFQNIMVNSMTILLLAAGQTFVIITSGIDLSVGFVMGFASVVSAKMMVSFYAMGF
                     PSGLAIISGVLITLLIGLLPGLLNGFLVTKMKVPPFIATFGMYGIAYGFSEIISYNVP
                     ITNLPPGVGDFGNGYLLYYLPGKFLSWFRQPAGLARAEIRELVPLVPNIFIISLLIIL
                     VFWFVLNRTQFGQHTFAIGGNVDAAQRAGINVDWHLMKIYMISSFFASLGGIMYVLRF
                     VNGRADAGSVRMLDSVAAVVIGGASLYGGTGGMIGTIIGTFIIGVLETGMVNLGIPTF
                     NKYIYVGVILIFAVLIDQFFPELVRKGD"
     gene            complement(2088..3155)
                     /locus_tag="PK171_03205"
     CDS             complement(2088..3155)
                     /locus_tag="PK171_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05242.1"
                     /translation="MDNVKTTQGLYVKLGKNWVILFLLLLCIIFSFVGKGFFANNTLN
                     NVLMSSSPSLLLAAGETFVIITGGIDLSIGFVKGMVSVISAIVMRNLFAMGYSPFICI
                     SLGIIVGLLIGLLPGLLNGLLVAKFRVPPFIATLGMYGIANGIAFGLCEGFPIIDLPQ
                     QAGRIGNGFILYYFSGLGFSFLKPPEGILDSQLRDLVRILPNSILISGIIIAIFGYIL
                     TKTQFGQHTYAIGGNIDAARRAGINVDSHLIKIYTISSFFGALAGVLSVLVYSIGSHT
                     QFSASLELFAVAAVVIGGASLKGGKGSIWGSFLGVMVLTVLEIGFLMAGIFPFYRYIA
                     VGSILVLAVIIDQTFPELVYD"
     gene            complement(3237..4214)
                     /locus_tag="PK171_03210"
     CDS             complement(3237..4214)
                     /locus_tag="PK171_03210"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT05243.1"
                     /translation="MKKSFIFILLVGILVFTFVVGVGAQEKKLKFAWCPTIEDPFYRM
                     IGKGIADKCEELGIDLFVTEYLKSWGAEIQVPVLEATAARGDIDLLITGPAAKDSLIA
                     PLKKIYDSGIEVLTVDTYLGDGDYSKESDYSFPLSFIGTDNFQGGVEVGKHLAEMIGG
                     KGKVFVSTTNPDVSSVVERVEGFKKGISDYPDIELVGVEYNLDNQEKAQQQTAAALQA
                     NPDIVGVFGTNLFSARGSYQAVVNAGLTGAVKIASWDATVDLINALKEGQVDLVLAQN
                     PYAMGQLAVEWGYKFFTEDAEVPKKLNPEFFFFTHENVNLPESQRYIYQ"
BASE COUNT         1472 a         1047 c          792 g         1228 t
ORIGIN      
        1 cttcattaag agattgccac gacctcaaaa aaccaggtct cgcaatgacg gtttttaatg
       61 atatagcatg gcaccaaatg aggataaaat gttaccccct cacctcatcg cttcctcttc
      121 ccctcagtct ttcttaaagg gaaaaagtga gggggaggga ggacttatta cccctctatg
      181 ctcacgacta actactcaca actcacggtt ttttattcat gttgaaccag ctcagcaccg
      241 accatcaaac tgactacttc atcgggattg gtttccttga ccatcaactc tcccactttt
      301 tcgccccgac gcaaaatcac caatcgatgc gcaacttgga aaacatcgta aagctgatgg
      361 ctgatgatga taatccctac tccctgggca cttaaagttc tcactagatc aagcaccttt
      421 ctttgttcag caacacccaa agcagcggtc ggttcatcca taatcaaaag tttggcattc
      481 cagtaaatgg aacgtgaaat tgccactgat tgacgttgac ctccggaaag cattttaatt
      541 ttatttttca aagaaggaat ttcgatccct aattgatcaa gaactttttt cgattcctgg
      601 tgcatataat catggtctaa aacgtcaatc aatcccaaag cttttttgag tttttctcgt
      661 cctaaaaaga tgttggaata gacattgaga ttttctgcta aggccaagtc ttgatagata
      721 gtctcaatcc ctaaacggat agcatccatg ggatttccga tttttacttc tttcccctga
      781 aatattacct gtccctcatc ctgatgataa actccggaaa cgaccttaat caaagttgat
      841 ttccctgctc cattatctcc gaggagtcca acaacctcac ctggcgcaac ctccatggaa
      901 accttattga ctgccactaa tcctccaaaa cgtttcacaa tatcttttac ttccaaaacc
      961 ggtatctttt gatcatccat actgtttaat ctccctttct gaccagttca ggaaaaaatt
     1021 gatcgataag tactgcaaag atgagaatca ctccaacata aatatattta ttaaaggttg
     1081 gtatccccaa attgaccatt cccgtctcca gcactcctat aatgaatgtt ccaataatcg
     1141 ttccaatcat ccctcctgtc ccaccataga gactagcacc accaatgacc accgcagcaa
     1201 ccgaatcgag catccggacg gaaccagcat cagctcgacc attgacaaat cgcagaacat
     1261 acatgatccc cccaagagaa gcgaaaaagg atgaaatcat gtagattttc attaaatgcc
     1321 agtcgacatt gattccggct ctctgggcgg catcaacatt ccctccaata gcgaaggtat
     1381 gctgtccaaa ttgagttcgg ttgagtacga accaaaaaac cagaataatc aaaagcgaga
     1441 tgataaagat gtttggcacc aggggaacca actcacgaat ctcagctcga gctagaccgg
     1501 ctggttgccg gaaccagctc agaaattttc cgggaagata atagagaaga tagccattcc
     1561 caaagtctcc aacccccgga ggaaggttgg taatcggaac attgtagcta atgatttccg
     1621 aaaaaccata agcaatacca tacatcccaa aagtagcaat aaagggaggg actttcatct
     1681 tggttactaa aaaaccatta agaagtccgg ggagaagccc gatgagtagg gttattaaaa
     1741 caccagatat tatggcaagg ccagatggaa atcccatggc ataaaaactc accatcatct
     1801 tggcggaaac caccgaagca aatcccatta caaacccaac tgacaagtcg attccactgg
     1861 ttattattac aaaggtttgt ccggccgcca gaagtaaaat ggtcatggaa ttaaccatta
     1921 tattctgaaa attcttggca ctaaaaaagc cggtccccaa aagactaaaa atgatgattt
     1981 ctcctattaa aaaaaggtaa acccagcttc ggcctattaa tactccccat tctcgcttgt
     2041 ttcctgataa tttacgaata tcttcagcac tcatggtatt ctctccttta atcgtaaact
     2101 aattcaggaa atgtttgatc aataattact gccaacacca atatacttcc cacggctata
     2161 taacgataga aagggaaaat tcctgccatg agaaaaccaa tttcgagaac cgtgagaacc
     2221 ataactccca aaaaagaacc ccaaatactt cccttccctc cttttaagct ggcccctcct
     2281 atgactaccg ccgctaccgc aaagagctcc aaactggcag aaaattgggt atgtgaacca
     2341 atggaataaa ccaagaccga caacacacct gcaagggcac cgaagaaaga tgaaatggta
     2401 taaattttaa tcaagtggct gtctacattg attcccgctc ttcgggcagc atcgatattt
     2461 ccaccgatag cgtaagtgtg ttggccaaat tgggttttgg ttaaaatata cccaaaaatg
     2521 gcaataataa taccggaaat aagaattgaa ttgggtaaaa tccgaaccaa atcccgtagc
     2581 tgagagtcaa gaataccctc aggtggtttg agaaaggaaa acccaagacc agaaaaataa
     2641 tagagtataa aaccatttcc tattcttccg gcttgttggg gaagatcaat aatgggaaat
     2701 ccttcgcata atccgaaagc aattccatta gctatgccat acattccaag agtggcgata
     2761 aaaggaggca ctcgaaactt ggcgaccaat agtccattga gtaacccggg taataaacca
     2821 atcaataaac ccacaatgat ccccaatgat atgcagataa agggagaata tcccatagca
     2881 aataaattcc gcataacaat agcagatata accgatacca taccttttac aaaaccaatg
     2941 gaaaggtcaa tcccaccagt tatgataaca aaagtttctc cggctgctaa aagtaatgaa
     3001 ggggatgaac tcattaaaac attgttcagg gtgttattgg caaaaaaacc ttttccaaca
     3061 aaactaaaaa ttatacacaa aaggagcaga aataagatga cccaattctt tcctaattta
     3121 acatacaaac cttgtgttgt tttaacatta tccatttttc accctggagt acagcacact
     3181 gggggggaag ataatgtctt ttccccccca gtgtgctgta gataattgaa gattatttat
     3241 tgatagatat atctctgaga ttccggtaaa ttaacgtttt catgggtaaa gaagaaaaat
     3301 tcggggttga gtttcttggg aacctcagcg tcttcagtga agaatttgta accccattca
     3361 acagccaatt ggcccatagc atatggattt tgagctaaaa ccaagtcaac ttggccttct
     3421 tttaaagcat tgatcaagtc tacagtggca tcccaagaag caattttaac cgctccagtc
     3481 aaaccagcgt taaccactgc ctggtaggaa ccacgagcac taaagaggtt ggtaccaaac
     3541 acaccaacga tatcgggatt ggcctggaga gctgcggcag tttgttgctg ggctttttcc
     3601 tgattatcca ggttgtattc tactccaacc aattcaatat ccggataatc agaaatacct
     3661 tttttaaaac cttctactcg ttccacaacg ctggatacat caggattggt ggttgaaaca
     3721 aatacctttc ccttacctcc gatcatttcc gcaaggtgtt ttccgacttc aactccaccc
     3781 tggaagttat cagtcccaat gaaagaaagt gggaaggaat aatcactctc tttggaataa
     3841 tcgccatcgc caagataggt atcaacagtg aggacttcta taccactgtc ataaattttc
     3901 tttaatggag caatcaacga gtctttagca gcaggaccgg taatgagcag gtcaatatct
     3961 ccccgggcag cggtggcttc caaaactgga acctggattt cagcacccca ggactttaaa
     4021 tattcagtaa caaagagatc aatacctaat tcctcacatt tgtcggcaat tcctttgccg
     4081 atcattctat aaaatggatc ttcaatagtt ggacaccagg caaatttcaa cttcttttct
     4141 tgtgctccca ctcctacgac aaaagtaaaa accagaattc ctactaaaag aataaaaatg
     4201 aatgactttt tcataaattt ccctcctaca tttttttgct tacgattggt ttataaaaac
     4261 tttagcttcc ataactagga aagctttatc acctcctcaa agatttttta aattcaattt
     4321 caaattattc taccataatt taacatcgaa tttcatcatc ttaaaaataa taaagaattc
     4381 tttcttgtta ttatatattt agaatctttt gttctgtaaa tttatctgca gacttgaaaa
     4441 acaaatcctt caaaagatat ttaaatggtg ggatgcaatt tttttgtgcc cggtctttat
     4501 ttaatgtagc gacatgccat ggcatgtcga atcttggat
//
LOCUS       DAOSOL010000091         4536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19188_length_4536_cov_3.414193, whole genome shotgun sequence.
ACCESSION   DAOSOL010000091 DAOSOL010000000
VERSION     DAOSOL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19188_length_4536_cov_3.414193"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1510
                     /locus_tag="PK171_03215"
     CDS             <1..1510
                     /locus_tag="PK171_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05244.1"
                     /translation="IEGLFDLSSGPDLIQWLEENELTGEVPGEVVLSREIHRDGRNRA
                     RINGRSVPVGLLKEAAAMLIDIYGQREHERFLANENQLNILDDFLDKTGNKERELYRQ
                     KYTDWLQTKHELEQLMAGDLSRWGELKFAFQEFEEMGLSPEKMNEIEEEFRLLANMQY
                     YCSALDAFFILMEGNESGEGITTLLSRLAYEVEKIPTEGKFLALNGIAENLRGIETEL
                     QEIALEVAALRSQLDYSTDKIEKIEKSVAEIEHLKRKYRCRTTSELIDYRKSIEEKLL
                     EVERQIEKKKQLEEQLEKYQRELLDSGEKLSQYRQKAAKIMKNRLEEELKQLAFSKVK
                     FEVSFDMVPVEQKRFWATGLDSISFMISLNPGQPLGPVMEVTSGGELSRLVLGIKSIA
                     LEMKNLPVMIFDEIDQGVGGKTAFWIGEKLRVIASGRQIVCVTHLPQIACFADQHLRV
                     DKHTDGQDTWAEISDLQGREKRLQELARMMGGEKSTVPALQFAETLMERAGK"
     gene            1565..1780
                     /locus_tag="PK171_03220"
     CDS             1565..1780
                     /locus_tag="PK171_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT05245.1"
                     /translation="MNKVLINQEFCKGCGYCVQICPKKVLVLSEGFNSHGYYPAMVND
                     GDHCIGCGFCAQVCPEVAISVYRENEK"
     gene            1777..2829
                     /locus_tag="PK171_03225"
     CDS             1777..2829
                     /locus_tag="PK171_03225"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOT05246.1"
                     /translation="MKKILMKGNDAVAEAAIQAGCDLYFGYPITPQTEISEYLSLRMP
                     EEGKIFFQAESEIASIYMVYGAAAAGKRVMTSSSGPGISLKQEGISYLASAELPAVIV
                     NMSRGGPGLGNIQPSQSDYFQAVKGGGHGDYKVIVLGPASVQELVDLTYLAFYLADKY
                     RNPVVILGDGMLGQMMEPVVFKKPDFPPLPVKDWATTGKDGRERHYVLCFDLDPRGLE
                     QFNNKIFKKYQKIEQEEIRYEVFGEDMPEMLLVGYGTIARILKSVVREAKNIGIRLAL
                     LRPITLYPFPSAIMQELAQQAGRVLDVEMNMGQMVEDVKLAVNGAVPVYFYGRSGGMI
                     PSVEDTLKRVRDILGK"
     gene            2841..3596
                     /locus_tag="PK171_03230"
     CDS             2841..3596
                     /locus_tag="PK171_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT05247.1"
                     /translation="MQTVFERPKTLINQPFRYCPGCNHGLAQRLIAEVIDELDIADKT
                     IGVGPVGCSVYIYECLDIDFVMGAHGRAPATATGIKRALPDRMVFSYQGDGDIAAIGT
                     AEIVHCAVRGECISAFFINNATFGMTGGQMAPTTILNQRTTTSPRGRKQDEAGFPFKL
                     GEMLAVAEGSAYIARVALTKPQLIKKAKQAIKRAFLTQQAGLGFSLVEILSPCPTNWA
                     MPPLEAVQWLEKNIIPVFPLGEIKVKEGVPDAR"
     gene            3586..4140
                     /locus_tag="PK171_03235"
     CDS             3586..4140
                     /locus_tag="PK171_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT05248.1"
                     /translation="MHVETVVAGFGGQGILFLGRVLATAGMIEGFHVSWYPSYGPEMR
                     GGTANCVVIISDQEIGSTISDHPQVCIVMNEPSMRRYAPTIRPGGLLVVNSSLITQKY
                     QDPNIQILEIPGNDIAGSEVGDPRTLNMVILGALIGYHSTVKEESIRQALEEILQGKK
                     SQLIDINMKAFSAGKNSISKKISE"
BASE COUNT         1390 a          746 c         1127 g         1273 t
ORIGIN      
        1 catcgaagga ctttttgatc tttcttccgg tcccgattta attcaatggc tggaagaaaa
       61 tgaattaacc ggtgaggttc ccggggaagt ggtgttgtct cgggagattc atcgagatgg
      121 gagaaatcga gctcgtatta acggaagatc ggttccagtt ggattgctaa aagaagcagc
      181 tgctatgtta attgatattt acggtcaaag agaacatgag cgattcttgg cgaatgagaa
      241 ccagctgaat atcttggatg attttttgga taaaactggg aataaagaaa gagagttata
      301 ccgacaaaaa tataccgatt ggctgcaaac caaacatgaa ttagagcagt tgatggccgg
      361 tgacctttcc cgttggggtg agctcaagtt tgcttttcaa gagtttgaag agatgggttt
      421 atctcctgaa aagatgaatg aaattgaaga agagtttcgt ttattggcta atatgcaata
      481 ttattgttcg gcgttagatg ctttctttat tttaatggaa ggaaatgaga gtggggaagg
      541 aataactact cttctttccc gtttggccta tgaagtggaa aaaatcccta ccgaagggaa
      601 gtttttggct ttaaatggta ttgcggagaa ccttcggggt atagaaactg agctacaaga
      661 aatagcttta gaagtcgctg ctttaagaag tcagttagac tattcgacgg ataaaattga
      721 aaaaatagag aaatcagtgg ctgaaataga acacttaaaa agaaaatatc gctgccgaac
      781 aaccagtgaa ttaattgatt accggaaaag tattgaagag aaactgttgg aagttgaacg
      841 tcaaattgag aaaaaaaagc aattggagga acagctggaa aaataccaac gagaattact
      901 tgatagtgga gaaaagcttt ctcagtatcg acaaaaagca gccaagataa tgaaaaaccg
      961 attagaagaa gaattaaaac aattggcttt ttccaaagtt aagtttgaag tcagttttga
     1021 tatggttccg gttgagcaga aaagattttg ggcaacaggt ttggatagta tcagttttat
     1081 gatttcatta aaccccgggc agcctttagg accagttatg gaagtcactt cgggtggaga
     1141 gttgtcacgt ttagtattag gtataaaatc aatagctttg gaaatgaaaa atcttccggt
     1201 tatgattttt gatgaaatcg accagggagt aggagggaaa accgcttttt ggatcggtga
     1261 gaaattaagg gtgattgctt ccggtcgtca aatagtttgt gttacccatc ttccccaaat
     1321 cgcttgtttt gccgatcaac acttgagagt cgataagcat actgacggtc aagatacgtg
     1381 ggcagaaata agcgaccttc aaggtcggga aaagcgtttg caagaattag cccgtatgat
     1441 gggaggagaa aaatcgacag taccagctct acaatttgcc gaaaccttaa tggaaagagc
     1501 cggaaagtaa gactcggtgc aattaactga gaaaatctgc tgattgacag gagagtagat
     1561 ggtattgaat aaagtcttaa ttaatcagga attttgtaaa ggttgtggat attgtgttca
     1621 gatctgtcct aaaaaagtgt tggttttatc agaaggtttc aattcacatg ggtactatcc
     1681 ggcaatggtg aatgatggtg atcattgcat tggatgtggt ttttgtgcac aagtgtgtcc
     1741 agaagtggca atttcagtct atcgggagaa tgaaaaatga aaaaaatttt aatgaaggga
     1801 aacgatgcgg tcgctgaagc agcaatccaa gcgggatgcg atctgtattt tggttatccg
     1861 attactccac aaaccgaaat cagtgaatac ctgtcgctga ggatgcctga agaggggaag
     1921 atttttttcc aagctgaaag cgaaattgcc tcaatatata tggtttatgg agcagcggcg
     1981 gctggaaaaa gagtgatgac ttcctcgtct ggaccgggaa ttagcctcaa gcaggaagga
     2041 atttcttatt tggcttcggc tgaattacca gcagtgatcg ttaacatgag ccgaggaggg
     2101 ccgggtttgg gaaacataca accttctcag agtgattatt ttcaggcggt aaagggagga
     2161 gggcatgggg attataaggt tattgtttta ggtcctgcat ctgttcaaga attagttgat
     2221 ctgacctact tggcattcta tcttgccgat aaataccgaa atccagttgt tattttggga
     2281 gacggaatgt taggtcagat gatggaacca gtagttttta aaaaacccga ttttcctcca
     2341 ttaccagtaa aagactgggc aaccactggt aaagacggta gagagcgtca ttatgtccta
     2401 tgttttgacc tggatccaag aggcttggaa cagttcaaca acaaaatatt taaaaaatat
     2461 caaaaaattg agcaagaaga aattcgctac gaagtatttg gagaagatat gccggagatg
     2521 cttttggttg ggtatggaac catcgctcgg attctcaaga gtgttgtcag ggaagctaaa
     2581 aatataggga ttcgtttagc acttctccga ccgattactc tctatccctt tccgtcagcc
     2641 attatgcagg aattagccca acaagctgga cgagttttag atgtggaaat gaatatggga
     2701 cagatggtag aagatgttaa attggcagta aatggtgctg ttccggttta tttctatgga
     2761 cggagtggtg gcatgattcc atcggttgaa gataccctaa aaagagttcg tgatatttta
     2821 gggaaataag gagaatagcc atgcagactg tatttgagag accaaagact ttaattaatc
     2881 aacctttccg ttattgtcct ggttgtaatc atggtttggc tcagcgatta attgccgaag
     2941 ttatcgatga actggatatt gctgataaaa ccattggtgt tggaccggta ggctgttcgg
     3001 tatatatcta tgaatgtttg gatattgatt ttgttatggg tgctcatggt cgagctccgg
     3061 ctactgctac cggtataaaa cgagcacttc ccgaccggat ggttttttct tatcaaggtg
     3121 atggtgatat tgccgctatc ggcactgcag aaattgtgca ttgtgcagta aggggggaat
     3181 gcatttcggc tttttttatt aacaatgcta cttttgggat gacgggggga cagatggcgc
     3241 ccactactat tcttaatcaa cggacgacaa cctcgcctcg gggaagaaag caagatgaag
     3301 caggatttcc ttttaagctg ggagaaatgt tggcagtagc cgaaggatcg gcctatatcg
     3361 ctcgagttgc cctaaccaag cctcagctga taaaaaaagc gaaacaagca atcaaacgag
     3421 cttttttaac ccaacaagct ggtttaggtt tttctctggt ggagatttta tcaccctgtc
     3481 ctaccaattg ggcaatgcct ccattagaag ccgtccaatg gttggaaaag aatattattc
     3541 cagtctttcc ccttggtgaa attaaagtca aggagggagt ccccgatgca cgttgaaacc
     3601 gttgtagcag gatttggtgg tcagggaatt ttattcttgg ggagagtatt ggccacagcc
     3661 ggtatgattg aaggatttca tgttagttgg tatccctcct acgggcctga gatgagaggc
     3721 gggaccgcta attgtgttgt catcatttcc gaccaggaaa taggatcaac catctccgat
     3781 catccccagg tttgtattgt gatgaatgaa ccatctatgc ggcgttatgc cccaactata
     3841 agaccaggtg ggttattggt tgttaattct tctttaataa cccaaaagta ccaagatccg
     3901 aatattcaaa tcttggaaat tcccggaaat gatattgccg gtagtgaagt tggtgatcct
     3961 cgaactttaa atatggttat tcttggcgct ttgatcggtt atcattcaac ggtcaaagaa
     4021 gagtcgatcc ggcaggcttt ggaagaaata ttacaaggaa agaaaagcca gttaatcgat
     4081 atcaatatga aggctttctc agcaggaaag aattcgatat ccaagaaaat ttctgaatag
     4141 aaccccgtag cacttgatag taccagatga ggatgataat aaagattcga catgccatgg
     4201 caggtcgcta cattagttaa agaatgggcg tgataaagac cgaggaggag aaaaagtaat
     4261 taaagaaaaa aagaaaaaat caaaagatga agtcctcacg ccctcacaaa gcgagggctc
     4321 aggatgacat tttttttaaa ttcttttgta ggggcttaat ttatcatgcc ccgtgggttt
     4381 tcgggatagc tacaatggtg atgagtaaga gactttttaa ggggttttga aaattcttaa
     4441 aaagaaaaat tccctggaag gggggattat ccatgttagt gagagaccgt atgagcaaag
     4501 atgttgtcct tattaccagt actactacca ttttag
//
LOCUS       DAOSOL010000092         4504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19344_length_4504_cov_3.759272, whole genome shotgun sequence.
ACCESSION   DAOSOL010000092 DAOSOL010000000
VERSION     DAOSOL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19344_length_4504_cov_3.759272"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PK171_03240"
     CDS             <1..313
                     /locus_tag="PK171_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOT05249.1"
                     /translation="HFVYPEYRLGHINEGLSSLVYSIAQSNFGLSKQKSLPQLCRKCP
                     YLFACRGECPKNRFLKTPDGEIGLNYLCSGLRKYFSHIDPYMQDMAKELMKTLPGYKL
                     R"
     gene            complement(442..1140)
                     /locus_tag="PK171_03245"
     CDS             complement(442..1140)
                     /locus_tag="PK171_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOT05250.1"
                     /translation="MKITVIYGQSHRGSSYHITQQILDKISNTNKEIYEYFMPNDTPS
                     FCVGCYQCIMKSEYACPQAEKVQNIVASMEVSDLIVINSPTYCLEMTGQLKTFFDHLG
                     YMWFSHRPKPVMFQKIGIVISTAAGGGSKKVAKSIAKQLFWMGVPKVYRLNKNIKGST
                     WQTVSDKMKGSIDRGTTKLSRKVEANIGKVRPGLGQRFLFIIMRSMQKSNNWNEVDKN
                     YWKENGWLDKKRPW"
     gene            complement(1459..2289)
                     /locus_tag="PK171_03250"
     CDS             complement(1459..2289)
                     /locus_tag="PK171_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05251.1"
                     /translation="MKRKNWLKSIVYIALLIISAIIVFPFIWLIITAFKSYPDIYAYP
                     ILYLPKNITFEHFRYVFVNLEFSKYFLNSLVLGAGTAVLTVLISILPSYATAKFNFFL
                     RKPVLLSILVCQMFPQIVFVVPFFLLLKYTGLINSYLGTMIVYLPFTTPIAVWMTRNF
                     FADIPQSLEESALIDGCSRFQAFYKISLPLVIPGIASVGIYAFIFSWSELMFSLSYLT
                     STDKQTVPVFLSLFIGQYQTRWGPLFAGSVVAALPPMIVFGFLQKYFIKGLTSGSVKE
                     "
     gene            complement(2295..3203)
                     /locus_tag="PK171_03255"
     CDS             complement(2295..3203)
                     /locus_tag="PK171_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05252.1"
                     /translation="MSSIIGNKHILKDKTGFRSDLIKNSFAYHSIIPYVVFMLILVVY
                     PVVQNVVVSFQVEDGWSFSNYREVLSDPLFSVVTLNTIIWTISCVILDLVVGLGIALL
                     LSQDVKGKMVFRTIILVIPWATPDIVAAVAWKWMYNDMYGVLNDILVKLGIIKDYLPW
                     LAVPTLAKAAVIIANLWKGFAISAMFYLAALQTVPRDLYEAAAMDGANLWQRFSKITI
                     PVIRPFILTTVMLTIIWTINYFPLIFVMTGGGPANATDTFVTHAYRMAFRFLDFSHST
                     AVSNINFIIVFIIAIIYTLSLIRKEG"
     gene            complement(3297..>4504)
                     /locus_tag="PK171_03260"
     CDS             complement(3297..>4504)
                     /locus_tag="PK171_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05253.1"
                     /translation="GTVFSAGEKITVWVGGHVVEQEETWKEVVADFTQQTGIEVEYQL
                     IGFEVYYDKLVAAYTAGQGPDVCFADLGGWVPTFAAQGWLEPIDEMLENSKVAEQIWP
                     NLWPTVTYQGKRYGLPWYTDCRLLLYNTKMFTDVGLDPNQPPVTWNDLLDTALKLTDE
                     SKRIYGYGVTGAKSEIATLAYMIFLFGADGEFLTEDYSKAAFNTPEGLEALQSYTELF
                     TKHKVSPPGTLSYTEDDYRTMMAQNRVAMAIGGPWSFPLIEMANPEIKGNYTVATHPY
                     KTTPASVLGGWASVIAQTSTQKEAAWKFVDFITSYDVWMMWIGKHSGPMPTRQDVCKD
                     APTFQADPKWQIIFETFPHAKVRPPIPQYPQISDQIQTMIQDVLTGKSTAEDALKKAE
                     EEVNKILTQ"
BASE COUNT         1399 a          923 c          847 g         1335 t
ORIGIN      
        1 tcattttgtt tatccggaat accgtttagg ccatattaat gagggtttga gcagtttggt
       61 gtactctatc gctcaaagta atttcggatt atccaaacaa aaatccctcc ctcagttgtg
      121 tcggaaatgt ccttaccttt ttgcttgtcg gggtgaatgc cctaaaaacc gttttctcaa
      181 aacccctgat ggtgaaatcg ggttgaatta tctatgcagc ggactgagga aatacttttc
      241 tcatatcgat ccctatatgc aagatatggc aaaagaactg atgaaaactc ttccgggata
      301 taagctaaga taataggagt tgctattcaa ttgggttgtt tcatgacatc cattctgtca
      361 atccatttga aggtttgata aatcaggctt cgacaaatga tttaaaaaac accaggcaat
      421 aaatttttga tttatttctc cttaccaggg ccttttctta tccaaccagc cattttcctt
      481 ccaataattc ttatcaacct cattccaatt gtttgatttt tgcatcgatc tcattatgat
      541 aaataaaaat ctttgtccta atccaggacg aactttccca atatttgcct ctacttttcg
      601 agagagcttg gtagttcccc tgtctattga ccctttcatt ttgtcagata ctgtctgcca
      661 ggtagaacct ttaatatttt tattcaatcg ataaacttta ggcactccca tccaaaacag
      721 ctgttttgct atagatttgg ccactttctt cgatcctcct ccggcagctg ttgaaataac
      781 aataccaatc ttctgaaaca tcaccggctt agggcgatga gaaaaccaca tataacccag
      841 atgatcgaaa aaagttttca attgaccagt catttcaaga caataggttg gagaattgat
      901 cacgatgagg tcagatactt ccatagaagc aacaatgttt tgtacttttt ctgcctgagg
      961 acaggcatat tcacttttca taatgcattg gtaacaacca acacaaaagc tcggcgtatc
     1021 attgggcata aaatattcat agatttcttt attggtattc gaaattttat caagtatctg
     1081 ttgtgtaata tgataactgc ttccccgatg tgactgccca tagatgacgg taattttcac
     1141 ttttttgtcc acctcggtat ttgtaaggag aatctttccg gtatcgtcgt ctaccagaat
     1201 tcaaatcgat attttatgaa tttcaactat actattcttt cttgaaaatg aagtcgcgaa
     1261 tgatacatat tgaacaataa ttgtctcttt tattttcatt ttctcctcat tagagagcat
     1321 ccttttaata ttttctttag gtttttctat tataatactt ctaatttttt aatgtctttt
     1381 caactcaaca aaacctcaat tctatagctt tattcaaaac ataatccttt cgttctctct
     1441 ccgaaatcgg tgtattcctc actctttgac cgaaccgctc gttaatcctt taataaaata
     1501 tttctgcaaa aaaccaaaaa cgatcatcgg tggcaaggca gcgaccaccg atccagcaaa
     1561 gagaggaccc catcgagttt gatactgacc aataaacagg gaaagaaaaa ctggaacggt
     1621 ttgcttatcg gtggaagtca ggtaagaaag cgagaacatg agctcgctcc aagaaaaaat
     1681 aaaagcgtaa atgcccaccg atgctatacc gggaataacc aagggaagac tgattttata
     1741 gaaagcctga aatcgactgc aaccatcgat cagtgctgac tcctcgagag attgagggat
     1801 atcggcaaaa aagtttctgg tcatccaaac cgcaatggga gtggtaaagg gaaggtaaac
     1861 aatcatggtt cctaaataac tattgatgag accagtatat tttaataata agaaaaaagg
     1921 gacgacgaaa acgatttgag gaaacatttg acacactaaa atggaaagca aaaccggctt
     1981 tctcaagaaa aaattaaatt ttgcggttgc gtaggaagga aggatggata tcaggacagt
     2041 tagcaccgcg gttcctgctc ctaacaccag actattgaga aaatattttg agaattctaa
     2101 gttgacaaaa acataacgaa agtgttcaaa agtgatattt ttgggaaggt ataaaattgg
     2161 ataggcataa atgtcggggt aggatttaaa tgcagtgatg atgagccaga taaagggaaa
     2221 aacaattatt gccgatataa tgagcaaggc aatataaacg atggatttta accagttttt
     2281 ccttttcatt caatttatcc ctctttccgg ataagactca aggtataaat aattgcgata
     2341 atgaagacaa taataaaatt aatattggaa accgcagtcg aatgtgaaaa atcaagaaac
     2401 cgaaaagcca tacgataggc atgggttaca aacgtatcag tggcattggc cggaccacca
     2461 ccggtcatga cgaagataag tggaaaataa ttaatggtcc aaatgatggt gagcataacc
     2521 gttgtcagga taaatggtcg aatgaccgga atggtaattt tactaaatcg ttgccatagg
     2581 ttcgctccat ccattgctgc tgcttcgtat agatctcgag gaacagtttg cagcgctgcg
     2641 agataaaaca tggccgaaat ggcaaagcct ttccagaggt tggcaatgat tactgctgct
     2701 ttggctaagg ttggtaccgc taaccaggga agatagtctt tgataatacc cagctttact
     2761 aaaatgtcat tcaacactcc atacatatcg ttatacatcc atttccaggc cacagcagca
     2821 acaatatctg gagtggccca gggaataacc agaataatag ttcgaaagac cattttcccc
     2881 ttaacgtcct gactcaatag gagagcaatc cctagaccta ccaccaaatc aaggattaca
     2941 caacttatcg tccaaataat agtatttagt gtaacaactg aaaaaagtgg atcagaaagg
     3001 acttctcgat aattgctgaa agaccaccca tcttcaacct gaaaactaac cacaacattt
     3061 tggaccactg gataaacaac caaaattaac ataaaaacca catagggaat aatagaatgg
     3121 taagcaaaag aattttttat caaatcagat cgaaaaccgg ttttgtcctt cagaatgtgc
     3181 ttattaccga taattgaact catataaatt gcaattcctt tccggctttg atgaaagtct
     3241 gggcagaggt agagatttct tacctatacc cctgcccctt tcctaattca atattattat
     3301 tgagttaaaa ttttattcac ttcttcttca gcctttttaa gggcatcttc agcagtggat
     3361 ttaccagtta aaacatcttg aatcattgtc tggatttgat cggaaatctg aggatattgg
     3421 ggtattggtg gccgtacttt cgcatggggg aaagtttcaa atataatctg ccattttgga
     3481 tctgcttgaa aagttggtgc atctttacac acatcttgcc tggtaggcat tggtccgcta
     3541 tgttttccaa tccacatcat ccatacatcg taactggtta taaaatcaac aaatttccag
     3601 gcagcctctt tttgggtcga ggtctgggca ataaccgatg cccaaccgcc caatacacta
     3661 gccggagttg ttttgtaagg atgggtggca accgtataat ttccctttat ttccggatta
     3721 gccatctcta taagcggaaa tgaccaaggt ccaccaatcg ccattgctac cctattttgt
     3781 gccatcatag tacggtaatc atcttcagtg taggatagtg tacccggagg agatactttg
     3841 tgcttggtaa aaagttcggt ataggattga agggcttcaa gcccttctgg agtgttaaat
     3901 gctgctttac tgtaatcttc agtaaggaat tccccatcag ctccaaatag aaaaatcatg
     3961 taagccagtg tcgcaatttc gctctttgct cctgttacac cataaccata gattcttttt
     4021 gattcatcgg ttaatttgag agccgtgtca agcagatcat tccaggtaac cggtggttgg
     4081 ttcggatcaa gtccaacgtc agtgaacatt ttggtattat acagtaacaa tcggcaatca
     4141 gtataccaag gtaaaccgta gcgtttccct tgataggtaa cagttggcca gagattaggc
     4201 catatctgct cggcgacttt actattttca agcatttcat ctatcggttc aagccatccc
     4261 tgagcagcaa aagttggcac ccatccacct aagtcagcaa aacagacatc aggaccctga
     4321 ccagcagtat aggctgccac taatttatcg taatatactt cgaagccaat caactgatat
     4381 tcaacttcaa ttccggtttg ttgggtaaaa tcggcgacaa cctctttcca ggtttcttct
     4441 tgttcaacta catgtccgcc aacccaaact gtaatttttt caccagccga gaaaacggtt
     4501 ccgg
//
LOCUS       DAOSOL010000093         4502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19348_length_4502_cov_3.749269, whole genome shotgun sequence.
ACCESSION   DAOSOL010000093 DAOSOL010000000
VERSION     DAOSOL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19348_length_4502_cov_3.749269"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1408
                     /locus_tag="PK171_03265"
     CDS             326..1408
                     /locus_tag="PK171_03265"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT05254.1"
                     /translation="MGSIYKHSRLFGFLLIAFLFFNFTNEGLAQKPAKISLPPSGYLY
                     HGVYPGPEDLSGEENLITLKSLKLYETAVRKKAAWVYFSHEWSQGEDFPIDTITWIQK
                     HGSIPFIRLMLRYKPQQNIKERVYTLDKIIQGNFDETLRAWAIAAHNYHNPLIVEYGT
                     EVNGEWFPWNARWYGKRKGTEKFKQAYRHIIDVMRDENANNITWVFHVNNLDLPDEEW
                     NRLEKYYPGNDYIDWIGVSIYGAQTPLSDDWPIFREALDAVYPRLVELAPDKPIVILE
                     FAMTSGHPHGNQAQWAKDALENLGLNRWPAVIGFSWWNEAWENDDDPNHNTNMRVQEN
                     VELSKIFQKYVGENDQVLDELILEHH"
     gene            1563..1991
                     /locus_tag="PK171_03270"
     CDS             1563..1991
                     /locus_tag="PK171_03270"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT05255.1"
                     /translation="MLNRTAQAIWEGNLKNGHGTMEFGGGAFKGNYSFTSRFENGKGT
                     NPEELIAAAHSGCFAMALSHMLAEAGYTPERVAATAKVSLEKVDDGFKITASHLDCIA
                     KIPGISQEKFMAIAEEAKKGCPVSVLLSNIQITLDARLEN"
     gene            2213..2530
                     /locus_tag="PK171_03275"
     CDS             2213..2530
                     /locus_tag="PK171_03275"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOT05256.1"
                     /translation="MRTGVANLPLHSGKAPAWLFQRMVKLSREIITLMVYEFGTRETL
                     KKFSDPYWFQALGCVLGFDWHSSGVTTTVCGAVKEGLRGLQSELKLVVAGGKGASSRR
                     FLC"
     gene            2556..3029
                     /locus_tag="PK171_03280"
     CDS             2556..3029
                     /locus_tag="PK171_03280"
                     /inference="COORDINATES: protein motif:HMM:NF017380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HOT05257.1"
                     /translation="MVAGDAESNRHAVAHLAKEKPEKIEAEAKNIVECNLSSRHPVTL
                     GDLNPRYFHKILLKTYERQPKNFQSLLEIEGVGPKTIRALALIAELLYGAPISTRDPA
                     RYSFAHGGKDGYPYPVDRPNYDRSIEIMKRAVEQSRLGNQEITNAIKRLQRFYQF"
     gene            3511..4170
                     /locus_tag="PK171_03285"
     CDS             3511..4170
                     /locus_tag="PK171_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05258.1"
                     /translation="MKNKGILIIIMALSLLTTLSSCSVYYFGFPPNTENVSPEVLDYV
                     KAHFYYCGQITRWNDGFIDVYDETNYKRMPEILKIWNEAIGGSTIFRLSHNPNSPIHI
                     FNQNGVFYESEEVCALTIDQNCINGWHRDTIVIINSNPNLFYNKFNIYLHEFGHVIGF
                     DNHTIGDDDIMGYTATTCVIPEIMVAAVRYVYGAKTDFSMEFKTANHSNSEVMAASGT
                     P"
     gene            complement(4260..>4502)
                     /locus_tag="PK171_03290"
     CDS             complement(4260..>4502)
                     /locus_tag="PK171_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05259.1"
                     /translation="LADKGKSEKKVEAPEVVKSKPQEDKMYQEMKDRIAALEAELKDC
                     QSLIVKLTAKTLKESKPPDNRGAIKRMTEWFVGKKE"
BASE COUNT         1412 a          844 c          876 g         1370 t
ORIGIN      
        1 ttcaacaatt cccctccttg gaggggtgcc gctttgcggc ggggagggtg cctttcggtt
       61 tttaatgttt attctgaaaa aaagagaaag aaatatatat aaaaagatga gatcctcacg
      121 gcttcgaaaa gcgaagcctc aggatgacgc ccccctcacc cactaactcc cctcggttat
      181 tttcaccctc agctggtacc gttacactgc atgaaggact attcaattaa aaagtgaaaa
      241 atacttaata aaatctcata gtattttttc ttttgacggg agtaaaatag gatgtaagta
      301 tatttaacaa aggagggtga aaaatttggg atcgatttat aaacattccc gcttgtttgg
      361 attccttttg attgcttttc ttttcttcaa cttcaccaat gaagggcttg ctcaaaaacc
      421 agcaaaaatt agccttccac cttcaggcta tttatatcat ggtgtctatc ccggtcctga
      481 ggacttaagt ggagaagaaa atcttattac actgaaaagt ttgaagcttt acgaaacagc
      541 ggtgaggaaa aaagcggcgt gggtatactt ttctcacgaa tggtctcaag gcgaggattt
      601 ccccattgat actattacct ggattcaaaa acacggatcg attcccttta tacgactcat
      661 gcttcgttat aagcctcaac aaaatatcaa ggaaagagtt tatacccttg ataaaataat
      721 tcaagggaac tttgatgaaa ctctgcgcgc ttgggctatt gctgcacata actatcacaa
      781 tcctttgata gtcgaatacg gaactgaagt aaatggtgaa tggttccctt ggaatgccag
      841 atggtatgga aaaaggaagg gaactgagaa atttaagcaa gcttatcgac atattatcga
      901 cgttatgaga gatgagaatg ccaacaatat aacctgggtt ttccacgtca acaacttaga
      961 cttgcctgat gaggaatgga atcgtttgga aaaatattac cctggtaatg attatattga
     1021 ttggattggt gtaagtatat atggagctca aacacccctg tctgatgact ggccaatatt
     1081 tcgagaagca ttggacgcag tctatccgcg attggttgaa cttgctccag ataaaccgat
     1141 tgtaattctt gaatttgcta tgacttctgg gcatccccat ggtaaccaag ctcaatgggc
     1201 aaaagatgcc ttggaaaacc ttggcctgaa tcgttggcca gcagtgattg gtttttcctg
     1261 gtggaatgaa gcctgggaaa atgatgatga ccctaatcat aataccaata tgcgagtcca
     1321 agaaaatgtt gaactaagca aaatattcca aaaatatgtt ggtgagaatg atcaggtttt
     1381 agatgaattg atattagaac atcattaaaa attactttcc atctcatttt ttcaaaatat
     1441 ttcttttttt aatgattttt aaatgaataa aatttggaaa ttcatttggg cttcaaagag
     1501 tgaaataatc atcagttttt agtgtttaac ctaataaaac ctaaagttaa ggagtgatcg
     1561 aaatgttaaa tagaacagca caagcaatct gggaaggaaa ccttaaaaac ggacatggaa
     1621 ccatggaatt cggaggaggc gcttttaaag gaaactattc ttttacctct cgttttgaaa
     1681 atggcaaagg taccaatcca gaagaactga ttgcagctgc ccattccgga tgtttcgcca
     1741 tggcgttatc ccatatgttg gctgaagccg ggtatactcc tgaacgggtt gcagctacag
     1801 ccaaagtgag ccttgaaaaa gttgatgatg gtttcaaaat cactgccagc cacctcgatt
     1861 gtattgccaa aattccagga attagccagg agaaattcat ggctattgca gaggaagcta
     1921 aaaagggatg ccctgtgagt gttttactct ctaatattca aatcactctg gatgctcgtc
     1981 tcgaaaacta attattaaat tatgatatag ggaatattga cctccttacc ttctctcgtc
     2041 aagtaacaag gaagaaaaga ggacaggttg ctcctcgcaa ccggctagag attataattt
     2101 ctaaaatagc ttggattgtc tctcttactt gacaagagaa ggtaaggatg aaggtataat
     2161 agatactggt tccagttttt ttagttcaag gattttgaag ggagaataaa ttttgcgaac
     2221 cggagtagct aatttaccac tccactcagg gaaagcacca gcttggttat ttcaacgcat
     2281 ggtaaagctt tcacgggaaa tcatcactct gatggtatat gaattcggaa ctcgtgaaac
     2341 cttaaaaaaa ttttccgatc cttattggtt tcaagccctc ggatgtgtat tgggcttcga
     2401 ctggcacagt tcaggagtca caactactgt ttgcggagcc gtgaaagagg gactacgagg
     2461 actccaatcg gaactcaaat tagtagtagc cggagggaaa ggggcttcct cacgccgctt
     2521 tctgtgctaa ccaccgggga aacattctct tgaatatggt tgctggcgat gccgagtcga
     2581 atcgtcatgc agttgctcat cttgctaagg aaaaaccgga aaagattgaa gctgaagcaa
     2641 aaaatatagt tgaatgtaat ttgtcaagtc ggcatccagt tactcttgga gaccttaatc
     2701 ctcgttactt tcataaaatt cttcttaaaa cttacgaacg ccaaccaaaa aacttccaaa
     2761 gtttattgga aattgaaggt gttggaccca aaactattcg tgctttggcg ctcatcgccg
     2821 aactcctcta tggagctcct attagtaccc gtgatccagc gcgttatagc tttgctcatg
     2881 gagggaaaga tggttatcct tatcctgtcg atcgacccaa ttatgatcgt tccatagaaa
     2941 taatgaagcg agcagttgag caatctcgtt tgggaaatca agaaatcacc aatgccatca
     3001 agcgtcttca gcgtttttat caattctaaa caaattaagg ttcaattttc ttcactctcc
     3061 tttttgggac ttctcgaaaa tcatttgggt ttcaaagctt atcaaaacaa tctaaaagtc
     3121 ctattaaaaa ccttctcttt actttataaa atagagtaga taattatgct gcatttgtag
     3181 caccagatga ggatgaaaat ataaactcaa aaaccaatct ccctctttag caaagggggt
     3241 tagggggatt tgattttatt gataaccaat cgaattccaa accatctccc tttatttaaa
     3301 gggagaggaa taaaaaagat tgaactcatg atattgccac attgtggctt ttcacaatga
     3361 cgaatttatg atacttctct cttgacagga gaaggctaat acaaggaagt ttaagcaatt
     3421 ttcagcactc aactcattta attctttgaa tcgaatcttg caattactcc gaaaaaagca
     3481 cccaaacttt ttttctgagg ttatgttcta atgaaaaaca agggaatttt gattattatc
     3541 atggcattat ccctcttgac aactctatcc agttgttcag tctactattt tggatttcca
     3601 ccaaataccg aaaatgtatc accagaagta ttggattatg tcaaggcaca cttttactat
     3661 tgtggtcaaa ttacccgatg gaatgatggt ttcattgatg tatacgatga aactaattat
     3721 aaaagaatgc cggaaatact gaagatttgg aacgaagcaa ttggaggatc gactatcttt
     3781 cgactaagcc ataatcccaa tagtcccatc catatattta atcaaaatgg agtgttttat
     3841 gaaagcgaag aagtctgtgc attaaccatt gatcaaaatt gcatcaacgg atggcatcgt
     3901 gataccatag ttatcatcaa ttctaatcca aatttgttct ataataagtt taacatttac
     3961 ttgcatgaat tcggtcatgt aattggtttt gataatcaca ccattggtga cgatgatatc
     4021 atgggatata ccgctacaac ctgtgtaatt ccagaaataa tggtagcggc agtaagatac
     4081 gtatatgggg caaaaaccga ttttagtatg gaattcaaaa ccgctaatca ttcaaactca
     4141 gaggttatgg ctgccagtgg aacaccataa aatttcattc aactatttta gttaaaataa
     4201 caaagtacca tttttaataa ttaaaaatga ttagtttaga aaaattaaca aattttaatt
     4261 tattcttttt tccctacaaa ccattcagtc attcttttaa tcgctcctcg attatctggt
     4321 ggtttactct ctttaagggt tttggcggtt aattttacaa ttaaactttg gcagtctttt
     4381 agttcagctt ctaaagctgc aatgcggtcc ttcatttcct ggtacatttt atcttcctgc
     4441 ggtttcgatt ttacaacttc aggggcttca actttcttct cactttttcc tttatcggct
     4501 aa
//
LOCUS       DAOSOL010000094         4495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19393_length_4495_cov_3.905180, whole genome shotgun sequence.
ACCESSION   DAOSOL010000094 DAOSOL010000000
VERSION     DAOSOL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19393_length_4495_cov_3.905180"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PK171_03295"
     CDS             complement(<1..662)
                     /locus_tag="PK171_03295"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT05260.1"
                     /translation="MKIGIDSYCYHRYFGEIYDFQQDPGKRITFDHFLKRSNELGVDV
                     VSLETCFFPENDQDFLKKLKDQIIEFGLDPVVAWGHPDGFEGGKKPEAAKDLEKHLRT
                     CQELNAQVMRIVVSSLAFRHEPHGPQIEKVSKILRDVMPRFEDNGVKIGIENHFDFTS
                     DELLEIIERVGSSNLGVTFDSGNALRIGENPIEAARKLIDKIFATHIKDVAPLYGGNP
                     AD"
     gene            880..1755
                     /locus_tag="PK171_03300"
     CDS             880..1755
                     /locus_tag="PK171_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT05261.1"
                     /translation="MVQIAIELKKIDVLKGRGVQDYAIINDPPFPANWQVSPHRHSQM
                     EIGLVKSESCTIQVGSERRYFERGDVFFFPARLAHGFATGSSQGVEIVVVQFTELEES
                     LTKQLMNLPPIGFFKLTDLEISVFLDLCYRLQREIVGSLPFAEMQCRALLEQIIVLLL
                     RTSSRGDLPYLIPQQQHAIEKALQIIHLRNQDNLRIGEIATEVGFSSQHFRELFKRYV
                     GVSPKKYLTTLKIQRSKCLLLHKEYSVTDVAYHLGFSSPQQFSKAFRKTIGLSPLLWR
                     RAYMLQDESALSEKY"
     gene            complement(1887..3062)
                     /locus_tag="PK171_03305"
     CDS             complement(1887..3062)
                     /locus_tag="PK171_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05262.1"
                     /translation="MKKINIHFPYAEILKRKARWEQVWNLQQPDRVPVLHYIGSRYWL
                     PLVGYENRFHDYLNDPGVMLEAQLLGAKWILENIKSDYHQIVFYPDFMWVEDVEPFGA
                     EIVYPEDDSPWVARPHFLQKNDNLDQLRSVDYINNRLHGKMISYYQEMKKQAQDYEIH
                     FSDGKIIPAVDCVMMGGGGIIGPTVIAGDLRSADNFAMDLYDRPDWVKDLLSIISEKA
                     IDWLNAAREIGGGKVAFCSSYLEGATFIGDDGTAQLSPKQYKEFALPALKKLAESIHN
                     QGLKVIAHNCGQADHLIDLWTNEVGIDIYYGFSYLTNKNLIKEIMGGKVILIGGIDTV
                     KLHGGKPADVREDVRQSLEVLKDCPGYIIMDGHNVAPGTPVENLNAMTDAAEEFGSF"
     gene            complement(3090..3929)
                     /locus_tag="PK171_03310"
     CDS             complement(3090..3929)
                     /locus_tag="PK171_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05263.1"
                     /translation="MSSKRKNEVTEQTKSHTIAIIVSLIMIIPFIWMILSSFKPGTEI
                     VKMPPTFIPHTFTLKNYQTLFERLPFGRYFLNSIIVSGGITIISMFSSSMLGYVFAKF
                     NFKFKNFFFFLLMSGFMIPFATLVIPMYLLTAKIGLTNNYLGLILPFCLSPFGMFLMK
                     QFMEDIPNDYMEAARLDGASELWIYSRIILPLTSAALGGVAIYNFLMTWNQLWWPLMV
                     TSQPAMRTLPLGVAALAQQQGKRYDLLITGASVSVLPIIVLFAVAQKQIVKGLSVSSG
                     LKM"
     gene            complement(3947..>4495)
                     /locus_tag="PK171_03315"
     CDS             complement(3947..>4495)
                     /locus_tag="PK171_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05264.1"
                     /translation="IGLFFTYLYQPQFGLFNALLSKIGFSRQGFLNNPQQAIYCVILT
                     EVWQALGFSTLIFLTGLNTIPDVFYEAAQIDGASAFVTFWKIIFPLARRVFLFITAIT
                     AISAFQAFDLVFVMTLSGGGTGGSSGGPGYSSYTMALQVYNDGILRSQTGIASAIAIM
                     LFIIIGVLTLLQLKILKPEWEY"
BASE COUNT         1424 a          931 c          789 g         1351 t
ORIGIN      
        1 cagtcggctg gatttccacc atataatgga gcgacatctt taatgtgggt agcaaatatc
       61 ttatcgatca atttgcgggc agcttcaatt ggattttctc ctatgcgaag ggcgtttcca
      121 gaatcgaaag ttaccccaag gttagaggaa ccaactctct ctatgatctc aagtaattca
      181 tccgaagtaa aatcaaagtg gttttctatg cctattttta caccattatc ttcaaaccta
      241 ggcatgacat cacgaagaat tttggatact ttttcgattt gaggaccatg aggttcatga
      301 cggaaagcta aactggaaac gacgattctc atcacttgag cgtttaattc ctgacatgtt
      361 cttaagtgct tttctaagtc tttcgctgct tcaggttttt tcccaccttc aaaaccatca
      421 ggatgacccc aagctactac tgggtccaaa ccaaactcaa ttatttgatc tttcaatttt
      481 ttcaaaaaat cttggtcatt ttctgggaaa aaacaagttt caagggaaac gacatctact
      541 ccaagctcat tcgatcgctt taaaaagtgg tcaaaggtta ttcttttacc aggatcctgc
      601 tgaaaatcat agatttctcc aaaataccga tgataacaat aactatcgat cccaattttc
      661 attgccctct ccttaacttt ggaatataaa ttttttcagc tgtctattgg ttacttttca
      721 caactgatac ctaggacaaa ccctccgttc aactaccatc ttttccttat ctatttttaa
      781 tcattcccat tttttttaca accatttttc gctgaatttc ttgtcaaaaa ggaagatggc
      841 cgatacaatg aatagatagt tatgaagata tgatatgggg tggttcaaat agctattgaa
      901 ttgaagaaaa ttgatgttct aaaaggtcgg ggggtacaag attatgctat tattaacgat
      961 cctcccttcc ctgctaattg gcaggtttca cctcatcgtc actctcaaat ggaaattggt
     1021 ttggtgaaat cggaaagttg cacaatccaa gttggttctg aaaggcgcta cttcgaaaga
     1081 ggcgatgttt ttttctttcc agccagatta gctcatggtt ttgcaaccgg ctcctcacaa
     1141 ggagttgaga tagtcgtcgt gcaattcact gaattggaag aatccttaac aaaacaattg
     1201 atgaatttac caccaattgg tttttttaag cttaccgatc ttgagataag cgtttttctc
     1261 gatctctgtt accgtctgca gagagaaatc gtaggttctc ttccctttgc tgaaatgcaa
     1321 tgccgagctt tattggagca aattatcgtc cttcttttaa gaacttcatc tcgtggtgat
     1381 cttccttatt tgattccaca gcaacaacat gctattgaaa aagctttgca aatcattcac
     1441 cttcgcaacc aggataattt acggatcgga gaaattgcta ccgaagtggg tttttcctct
     1501 cagcactttc gtgaactttt taaacgatat gtaggcgtca gcccaaagaa atacctcact
     1561 accttgaaga tacaaagaag taaatgtctt ctccttcata aagaatactc cgtcaccgat
     1621 gtggcatacc acttaggatt tagctcgcct cagcaatttt ctaaggcttt tcgaaaaacc
     1681 attggcttaa gccctcttct gtggcgaagg gcttatatgc ttcaagatga gagcgcatta
     1741 tcggaaaaat attagtaatt tatgcaaaaa gtgaatctca gattttcctt aagatataaa
     1801 taaaaactcg ctctcctgag cgactggggt gaaagagagc gaggtaaaaa tagcacacat
     1861 tttaaatcct tttcttggcc aaaccgtcaa aaacttccaa attcttcggc tgcatcagtc
     1921 atggcattta aattctcaac tggagttccg ggggcaacat tatgtccgtc cataattata
     1981 tatcctggac aatccttaag aacctcaagg ctttgccgga cgtcttctcg aacatcagct
     2041 ggttttccgc catggagttt taccgtatct attccaccaa tcaggataac tttcccaccc
     2101 attatttcct tgataaggtt cttattggta agatagctaa atccataata gatatcgatt
     2161 cccacctcat tggtccacaa atcaataagg tgatcagctt gtccacaatt atgagctatt
     2221 actttcaaac cctgattatg aatactttct gccaattttt ttaaagcagg aagagcaaat
     2281 tctttatatt gttttggtga taattgagca gtaccatcgt ctccaataaa ggtagctcct
     2341 tccaaataag aactacaaaa agcgaccttt cctcccccaa tttctctggc agcattgagc
     2401 caatcgattg ctttctctga gataatcgac aataaatcct tcacccaatc aggacgatca
     2461 tataaatcca tagcaaaatt atcagcactt cttagatctc ctgctataac cgttggacca
     2521 ataattcctc caccacccat catcacacaa tcaacagcgg gtataatttt tccgtcggaa
     2581 aaatgaattt cataatcctg ggcttgcttt ttcatttcct ggtaataaga aatcatcttt
     2641 ccatgaagtc ggttatttat ataatcaacc gaacgaagct gatccaaatt gtcgtttttt
     2701 tgcaaaaaat gaggacgggc tacccaggga gaatcatctt ctggataaac aatttctgca
     2761 ccaaaaggtt caacatcctc cacccacata aaatctggat aaaatacgat ttggtgataa
     2821 tcagacttga tgttctctaa aatccatttg gctcctaaaa gctgagcttc caacatcact
     2881 cctgggtcat tgagataatc atggaatcgg ttttcatacc caactaaagg caaccaatat
     2941 ctcgaaccaa tataatgcaa aaccggaact cggtcaggct gctgcaaatt ccaaacttgt
     3001 tcccagcgag cttttctttt cagtatttca gcataaggaa agtgaatatt tatctttttc
     3061 atataaatcc tcctgcttat caatatcaac tacattttca aaccacttga aaccgataac
     3121 ccttttacaa tttgtttttg agcaacggca aataagacaa tgatgggtaa aactgaaacc
     3181 gaagctccgg taattaataa atcgtatctc ttcccctgtt gttgagccaa agcagcaact
     3241 cctaagggta aggttcgcat tgctggttga gaagtaacca ttaaaggcca ccaaagctga
     3301 ttccaggtca ttagaaaatt ataaattgca actcccccta aggcggcact ggttaagggt
     3361 aaaataatac gactataaat ccatagttca gatgcaccat ctaaacgggc ggcttccata
     3421 taatcgttgg gaatatcctc catgaactgt ttcatgagaa acataccgaa agggctgagg
     3481 caaaaaggca aaataagccc taaatagtta ttggtcaatc caattttagc agttagaaga
     3541 tacataggaa taaccaaggt tgcgaaagga atcataaaac cgctcatcag gagaaaaaag
     3601 aaaaagtttt taaatttaaa gttaaattta gcaaacacat aacccagcat cgaactggaa
     3661 aacattgata ttatggttat tccacctgat acaattatgc tattcaggaa atatctacca
     3721 aatggaagtc tttcaaataa agtttggtaa ttttttaaag taaaagtatg ggggataaaa
     3781 gtgggaggca tttttacgat ttcggttcct ggtttaaaag atgacagaat catccaaata
     3841 aaagggatta tcataataag agaaacaatg atagcgatcg tatgggattt ggtttgctca
     3901 gttacctcgt tttttctttt tgaagacatt ttaatcctcc cactctttaa tattcccatt
     3961 ctggtttaag aattttaagc tgtaaaaggg ttaatacccc aataatgata aagagcatta
     4021 ttgcaatagc tgaagctatc ccggtctgag atcgaagtat tccatcgtta taaacttgca
     4081 gagccatggt ataactactg taaccagggc cacccgatga acctccagtt ccaccaccgg
     4141 ataaggtcat cacaaaaact aaatcgaaag cctggaatgc agaaatagct gtaattgctg
     4201 ttataaaaag gaacactctt cttgccaaag gaaagattat tttccaaaag gtaacaaagg
     4261 cgctggcacc atcaatctga gccgcctcat agaaaacatc gggtatggta ttgagtccag
     4321 ttaagaatat taaggttgag aatccaagag cttgccaaac ttcagttaaa attacacaat
     4381 aaattgcttg ttgaggattg ttcagaaaac cttgccttga aaaaccaatt ttactgagta
     4441 aggcattaaa taatccaaat tggggttggt agaggtaggt aaaaaaaaga cctat
//
LOCUS       DAOSOL010000095         4490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19428_length_4490_cov_3.880496, whole genome shotgun sequence.
ACCESSION   DAOSOL010000095 DAOSOL010000000
VERSION     DAOSOL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19428_length_4490_cov_3.880496"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1372
                     /gene="rho"
                     /locus_tag="PK171_03320"
     CDS             170..1372
                     /gene="rho"
                     /locus_tag="PK171_03320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT05265.1"
                     /translation="MGIVGYSRKRKSELIFEILKKATESKGYIFSEGILEITIEGYGF
                     LRTSDDFTVSENDVYVSPSQIKRFALSSGDKVAGQVRPPKEGERYYALLRIEAINDED
                     PEQAKKRPLFENLTPIFPNLQFVLETTPNEISTRIIDLFSPIGVGQRGLIVAPPKAGK
                     TVLLEKIANGITTNYPNVVLIVLLIDERPEEVTQMERSVKGYVIASTFDQKPENHIRV
                     AELTLERAKRLVEEGKDVVILLDSITRLARTNNLVVPNTGKTLSGGIDSSALHWPKRF
                     FGAARNIEEGGSLTILATALVETGSRMDEVIYEEFKGTGNMELHLSRALSESRIFPAI
                     DIHRSGTRKEELLIKQNDLERIWMLRKLLANVDQQEGAQMVIDRMKNTKSNREFLKMI
                     DHVLKNSR"
     gene            1539..1745
                     /gene="rpmE"
                     /locus_tag="PK171_03325"
     CDS             1539..1745
                     /gene="rpmE"
                     /locus_tag="PK171_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOT05266.1"
                     /translation="MKKDIHPNYKKTRVVCACGNSFVTRSTKTPEIRVEICASCHPFF
                     TGQQKLIDTAGRVEKFRAKYGDNF"
     gene            2101..2757
                     /gene="thyX"
                     /locus_tag="PK171_03330"
     CDS             2101..2757
                     /gene="thyX"
                     /locus_tag="PK171_03330"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOT05267.1"
                     /translation="MKVTLISSTPNPELTIARAARLCYRRSYDEDITLEKAQKLIREI
                     VARGHESVLEHASFTFLIGGISRAASHQLVRHRIASYSQQSQRYIHFKNPEFVIPPSI
                     KEDETLFDHFREQIQKSSDIYQEMIKMGIAEEDARYILPQAITSQIILTANAREYLHI
                     LKLRLCSRSQWEIRMIATAIFKILKELSPIIFESAGPPCATGICPEGDKGCGHPWSKR
                     "
     gene            2730..3635
                     /locus_tag="PK171_03335"
     CDS             2730..3635
                     /locus_tag="PK171_03335"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOT05268.1"
                     /translation="MWTSVEQKIKPEKNERHNVGGQAIIEGVMMRSPWKISIAVRKPN
                     GTIVTDIKEKPILSKTNKFFALPIIRGVVNMVDSLIIGLKALSLSATLAMDEEEEKLS
                     AFDLSIAMLLAFGLFVGLFIALPTFLTSRIDHYIHSTILYNIIEGLIRVTIFLLYLFI
                     ISRMKDIKRIFEYHGAEHKTIFAFENGQELTLENIRKYTTHHPRCGTNWLMIVMIISI
                     FIFSFLGRPGIITRIISRIALIPIVAGLAYEAIRLLSKYQDRGLANILALPGLLLQRI
                     TTQEPDDSQLEVAVAALKGSLKEEC"
     gene            3637..>4490
                     /gene="prfA"
                     /locus_tag="PK171_03340"
     CDS             3637..>4490
                     /gene="prfA"
                     /locus_tag="PK171_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT05269.1"
                     /translation="MWQNKAEELNSRYLELTHLLSDPQITQDLQRYRQLTKAISEIEE
                     TAKKYQEYQAAQKNLEEDRNFLKTEQDADLRELLEEEIKALENKLIELEEEIKYLLIP
                     RDPNDKRNTLVEIRAGTGGEEAALFVSDLFRMYSRYAENQGWKCEVMSTNPTELGGFK
                     EIIFAIEGDGAYSKFKYESGVHRVQRVPITEAGGRIHTSTVTVAVLPEAEDVDIDINP
                     EDLRIDVYRSSGHGGQSVNTTDSAVRITHLPSGMMVTCQDEKSQHKNKAKALRILRSR
                     LLEIEQQK"
BASE COUNT         1505 a          803 c          908 g         1274 t
ORIGIN      
        1 aggaggtaca attgcattga gtacagacag caatcaagtt gatttagaat tagaggaaaa
       61 agagttagaa aaagaacctg aaaaagaaaa aaccagagcg gaattgcagt tttccttagg
      121 agaactaaaa atcaaatcca ataacgaact gggagatatt gcccaaaaaa tgggtatagt
      181 tggctatagt cgaaagagaa aaagcgaact gatatttgaa atattaaaaa aggcgactga
      241 atcgaaaggt tatattttca gcgaaggtat tttagaaatc actatcgaag gatatggttt
      301 tctccgaact tcagatgatt ttactgtcag cgaaaacgat gtttacgttt caccatcaca
      361 aatcaaacgt tttgctttaa gtagtggtga taaagttgcc ggacaggtaa gaccaccaaa
      421 agaaggggaa agatattatg ccctgcttcg aattgaagct attaacgatg aagatccaga
      481 gcaagccaaa aaaagacccc tttttgaaaa tttaacccca atttttccca atctccagtt
      541 tgtattagaa actacaccca atgaaatttc tacaagaatc atcgatcttt tttctcctat
      601 tggtgtaggg cagcgaggtc ttattgtagc accaccaaaa gctggaaaaa ccgtattgtt
      661 ggaaaaaata gccaatggga ttactaccaa ctaccccaac gttgttctga ttgttttatt
      721 gatcgacgaa cgtcctgaag aagttaccca aatggaacgc tcggtgaagg gttatgttat
      781 tgcctcaact tttgaccaaa aaccggaaaa tcatattcgt gttgcagaac ttactttgga
      841 aagggcaaaa cgattggtag aagaggggaa ggatgttgtc attttgcttg acagtataac
      901 ccggttggca cgaaccaata atttggtcgt tcccaatact ggaaaaaccc tttcaggtgg
      961 aattgattct tcagctctcc attggccaaa aagatttttt ggagctgctc ggaatatcga
     1021 agaaggtggg agtctgacca ttttagcaac cgcactggtt gaaaccggat cccgtatgga
     1081 tgaagtcatt tacgaagaat ttaagggaac cggaaacatg gagcttcatc ttagccgggc
     1141 tttatcggaa agtagaatat ttccagccat tgatatccat cgatcaggga cacgaaaaga
     1201 agagctgctg attaaacaaa atgatttaga aagaatttgg atgttgagaa agcttttagc
     1261 caatgttgat caacaagaag gagctcaaat ggttatcgac cgtatgaaaa atacaaaatc
     1321 taatcgagaa tttttgaaaa tgattgatca tgtgttgaaa aactcccgtt aattttgaca
     1381 ctcaaaatca gtatgtgata taatacccag cgtttttaag tcatttttgt tcattcttca
     1441 attcctgatg tataaaaggt accaatgaat tattaccttt ttttaaatca atttttgata
     1501 cctatatact taaaacggaa ttgggtgagg tgataacaat gaaaaaagat attcatccca
     1561 attataaaaa gacacgagtt gtttgtgctt gtgggaatag ctttgtaact cgttctacca
     1621 aaacaccgga aataagagta gaaatttgtg ccagctgtca cccattcttt actggacagc
     1681 aaaaattaat cgatactgct ggtcgagttg agaaattccg ggcaaaatac ggcgacaatt
     1741 tttagcttta gttacgaaat gaataaaaaa gggtagtgtg tataatttac atactccctt
     1801 tttttattgg aataaaaaag caatgacgag ctaaaaggta ttgaacaatg acctccataa
     1861 gcgttagatt gctatttgta ctgcatcgtg acgcaagatg agaatgagaa taaaaaattt
     1921 tctctctcac cccaatccct ttacatcatg gaaaaaggga ataacaacaa agaacaactt
     1981 aaggaggagt ccttttgtaa attacttctt cttcaagtgg agtaaaatac gatgagagtg
     2041 aaattcaaac tcatcaccag atactaatca ctcatcatta tttataaaag gagtaggggt
     2101 atgaaggtaa cgcttataag ctcaacaccg aatcctgagc taaccattgc aagagctgca
     2161 cgtctctgct atcgaagaag ttatgatgaa gacataacat tagaaaaagc tcaaaaactt
     2221 atacgagaaa tcgtagcccg tggccatgaa tcggttttag aacacgcttc ttttaccttt
     2281 ttaattgggg gaatttcccg agctgcttca catcagttgg ttcgtcatcg catagcttcc
     2341 tactctcaac aaagccaacg atatattcat tttaaaaatc cagaatttgt cattccgcct
     2401 tcaattaaag aagatgaaac cttatttgat cattttcggg agcagataca gaaaagttcc
     2461 gatatatatc aagaaatgat taaaatggga atagcagaag aagacgctcg ttatatatta
     2521 ccacaggcta ttaccagcca aattatatta acggccaatg cccgtgaata tctccatatt
     2581 ttaaagcttc ggctttgtag tcgatctcaa tgggaaataa ggatgattgc caccgcaata
     2641 tttaaaattt taaaggaatt atcaccaatt atttttgaaa gcgccggtcc gccgtgtgca
     2701 accggaatct gccccgaagg ggataaagga tgtggacatc cgtggagcaa aagataaaac
     2761 ccgaaaagaa tgaaaggcat aatgtaggag ggcaagcaat tattgaaggg gtgatgatga
     2821 gaagcccttg gaaaatttca attgcggtta ggaaacccaa cggtacaata gtgaccgata
     2881 ttaaagagaa accgatttta tcaaaaacta ataaattttt tgctttaccg attattcgtg
     2941 gtgttgtcaa tatggttgat tcactcatca tcggattgaa agctttgtct ctgtcagcaa
     3001 ctttagctat ggatgaagag gaggaaaaat tatcggcttt tgaccttagc atcgccatgt
     3061 tgttggcttt tgggttattt gtaggattgt ttatagcact tccaactttt ttaacctctc
     3121 ggattgatca ttatattcat tcaacaatcc tctacaatat tatcgaaggg cttattcgag
     3181 taacaatttt tttattgtat ctctttatta tttctcgaat gaaagatatt aaaagaattt
     3241 tcgagtacca tggagcagaa cataaaacta tatttgcttt tgaaaatggt caggagctta
     3301 ctctcgaaaa tattcgaaaa tataccaccc atcatccccg ctgtggaacc aattggttaa
     3361 tgatcgttat gattatcagc atttttattt tttctttttt aggtcgtccc gggataataa
     3421 ccagaatcat tagccgaatt gctctcattc caattgttgc aggattggct tatgaagcca
     3481 ttcgcctttt gtcaaaatat caggatcgtg gtttggctaa tatattagcc ttgcctggtt
     3541 tgctccttca aagaattacc acccaggaac cggacgattc acagttagaa gttgctgtgg
     3601 cagctttaaa aggaagcctt aaagaggagt gttagtatgt ggcaaaataa agctgaggag
     3661 ttaaatagcc gatatcttga gctcacccat ttattatcag atccccaaat tactcaagat
     3721 ttacaacgct atcgacaact cactaaagct atttctgaaa ttgaagaaac tgctaagaaa
     3781 taccaagaat atcaagcagc tcaaaaaaat ttagaagaag atagaaattt tctcaaaact
     3841 gagcaagacg cggatttacg tgagctctta gaagaagaaa ttaaagcatt agaaaataag
     3901 ctgattgagc tggaagagga gattaaatat ctacttattc cccgcgatcc taatgataaa
     3961 agaaatactc tggttgagat acgagcggga accggtggag aggaagctgc actttttgtc
     4021 tctgacctct ttagaatgta ttcccgttat gctgaaaatc agggttggaa gtgcgaagtc
     4081 atgagtacta acccaaccga gctcggtggt ttcaaggaaa ttatttttgc catagagggt
     4141 gatggtgcct atagcaaatt caaatatgaa agcggagttc atcgagtgca aagagttcca
     4201 ataaccgaag ctggtgggag aatacacact tcaactgtaa ctgtagcagt tctccctgag
     4261 gcggaagatg tcgacatcga tatcaatccg gaagatttac gcattgatgt ttatcgttca
     4321 tccggccatg gagggcaaag tgttaatacc accgattcag cagtgcggat tacccacctt
     4381 ccttccggca tgatggttac ctgccaggat gagaaatccc agcataaaaa taaagccaaa
     4441 gctctgcgaa ttctccgttc aaggctttta gagattgaac agcaaaaaca
//
LOCUS       DAOSOL010000096         4473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19521_length_4473_cov_4.340426, whole genome shotgun sequence.
ACCESSION   DAOSOL010000096 DAOSOL010000000
VERSION     DAOSOL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19521_length_4473_cov_4.340426"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PK171_03345"
     CDS             <1..1068
                     /locus_tag="PK171_03345"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOT05270.1"
                     /translation="IAIIAGPCAGGAVYSPALMDYIFMIQGTSKMFVTGPQVVKAATG
                     EEVTAEELGGAHTHATKSGVASFVSRSEEECFFEVKRLLSYLPSNNAEEPPVGECHDP
                     VDRKNTSFLDIVSTNPNRPYNVKKIIEDLVDQGSFFEVQKDFAKNLIIGFARMNGHTV
                     GIVANQPYHLAGCLDIDSGDKASRFIRFCDAFNIPLVTLVDVPGFLPGSNQEFGGIIR
                     HGAKILYAYSEATVPKITVIMRKAYGGAYLAMCCRDLGADLVLAWPTAEIAVMGAEGA
                     VNIIFRKEIETALDTQVERERLLEEYRQEFYNPYEAAKRGYVDQVIDPQETRLKVVRA
                     LEIFWSKRVTGKGKKHGNIPV"
     gene            1049..1252
                     /locus_tag="PK171_03350"
     CDS             1049..1252
                     /locus_tag="PK171_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05271.1"
                     /translation="MGISQYKVEAKVNPQIIAAVIAAVLQYEHDEQPGEIIIEKQYIQ
                     QKNKGWKNLCLQENCWGLSRFRY"
     gene            1933..2316
                     /locus_tag="PK171_03355"
     CDS             1933..2316
                     /locus_tag="PK171_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05272.1"
                     /translation="MRKFKVRVNGEEFEVEVSEVNESREDGKIRVEKIKRIPHEITAT
                     SNPMQVTTVLRAPMPGRVLSVQVKKGERVSGGQVGIILEAMKMENEIQIENEGIVTQI
                     FVEENDTVDTGDPLLECEENPRDIS"
     gene            2313..4196
                     /locus_tag="PK171_03360"
     CDS             2313..4196
                     /locus_tag="PK171_03360"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HOT05273.1"
                     /translation="MKYSKLFLLIAIISALLGAIMVLPTRLRNESAMKEIEVVCDWQD
                     IEVISLRLGISVEETLDELKQAGVSVIGVSDYDLKNLQQLGKVLPVVVDHDYPSILRY
                     FYVSNSTLKETIIHHLNILGRVPVEVSPNIIGLNISYEEEDKIGLGWDYNLVQLLKDK
                     GFRIVLRPRNWQGISPEILKAVLSDPIFDQADGLIFFGDQVLGNGNEESLKEMANFLD
                     EKKLFWGYTEFVGQKGETILARQVPGQTIRVHSIPPDEIKNYTPLEARERFLRAVKER
                     SVRLLYVRFFTEPTINLGEKNYSYLTALFSDIESSGFTRGTIHPLIPFAPPLIASVLF
                     SVSVAIAIALLYSYFLPGKWIILILSLFVLLGALFQDQMLSLRIIGVLAGIAYPSLAV
                     FSLIDGLRSKKNPLVSILVAFCMVFAGGLVVSCGLYHWLYVLRIEQYSGVKTSLVIPI
                     LLVVLYLFRSGYLNRSIRSVVLGTLKRYEFVILMILAGGFVVYLTRSGNFPLLPAGML
                     ESKMRLWLERLLFMRPRTKEFMIGYPALWLLISLRRFNYQPLFKVVLWLGVTIGFITF
                     FNSFCHIHTPFLFILLRFFNAFILSLVIFIFYYLIVRIAYWIWKWLGQFGENDAHKKS
                     TNI"
     gene            4208..>4473
                     /locus_tag="PK171_03365"
     CDS             4208..>4473
                     /locus_tag="PK171_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05274.1"
                     /translation="MKDTQKKIFLLGYYGFGNWGDELSLQSIINDLEIISKDTPYSFL
                     YSVLNQDNRFFPRSFSNLSVIIRKKIITVLKEITKSDYVVVGGG"
BASE COUNT         1328 a          768 c          975 g         1402 t
ORIGIN      
        1 atagcaataa tagctggtcc ttgtgccgga ggagcagtat attctccagc tcttatggat
       61 tatattttta tgattcaagg cacaagtaag atgtttgtaa ctggccctca agttgtgaaa
      121 gcagcgaccg gcgaagaagt cactgccgaa gaattgggtg gtgcccatac tcacgctacc
      181 aaaagtggag ttgccagttt tgtatccaga agtgaagaag agtgtttctt tgaagtaaaa
      241 aggcttcttt catacctacc ttctaataat gccgaagaac ctccagtagg agaatgccat
      301 gacccagttg atcgaaaaaa tacctctttt ttggatattg tatctaccaa tcccaatcga
      361 ccttataatg ttaaaaaaat cattgaagat ttggttgatc aaggttcttt ttttgaagtt
      421 caaaaagatt ttgcgaaaaa tttaatcatt ggatttgctc gaatgaatgg tcatactgtg
      481 ggtatagtgg caaaccaacc ctatcatctg gctggatgtt tagatatcga ttctggtgac
      541 aaggcgtctc ggtttataag gttttgcgac gctttcaaca ttcctctggt tacgcttgtt
      601 gatgttccag ggtttttacc aggaagtaat caagaatttg gaggaatcat tcgacatgga
      661 gcaaagattc tctatgctta ctcagaagcc accgttccca agattacggt aataatgcga
      721 aaagcatatg gtggtgctta tttagctatg tgttgtcgtg atctcggagc tgatttggta
      781 ttagcttggc cgactgctga aattgccgta atgggtgccg aaggggcggt caatatcatt
      841 tttcgcaaag agatagaaac agctttagat actcaggttg aaagagagag attgcttgag
      901 gaataccgtc aagaattcta taatccttat gaagccgcca aaagagggta tgtcgatcaa
      961 gttatcgatc ctcaagaaac tcggctaaag gttgttcgag ctcttgaaat tttttggtca
     1021 aaaagagtta cgggaaaggg aaaaaagcat gggaatatcc cagtataaag ttgaagccaa
     1081 ggttaaccct caaataatag ctgcagtgat agctgcggtc cttcaatatg agcatgatga
     1141 acaacccggg gaaataatta tagaaaaaca atatatccaa caaaaaaata aagggtggaa
     1201 gaatttgtgc ttgcaagaaa attgttgggg tttatcgcgt tttaggtatt aatttccatc
     1261 agcaattaaa gtaaaaactc aaaaatcaga aagaatgatt ccgttattcc ttagcgctaa
     1321 ataaagatga aaacccaaga ttcgacatgt catggcatgt cgcaacaata aagaaacacc
     1381 gggcgcaata aagaccgagg ggggcgaata tttgattcct tctcccttga tgggagaagg
     1441 tgaggatgag ggtgaagatc cagaatgaga ttatgattgg ttgctataaa tacgttaatt
     1501 gtaaaaatcg ttccggtatt atcaggatag accctcatgc catttttgtg gtaccagatg
     1561 aggatgaaaa ttgcttattc agaaatcaat ccccccttta gaaaaggggg ttagggggat
     1621 ttgatttttt ttcccgctcc gtcattgaga ggagcgcagc ggacgtggca atctcatctt
     1681 taaaatattt taaaaaaaag gaaaaaataa aaagatgaga tcctcacgcc ctcgaaaagc
     1741 gagggctcag gatgacgcgt gtagcgtaag atgagatcct cacggcttct gaatacgaag
     1801 cctcaggatg acacatgtga tgtcagatga gatcctcacg ccctcaaaaa acgatggctc
     1861 aggatgaagc ctttattaat cgttcatgtc ttttcaggat agcaggtatt attaatgggg
     1921 aggagtttca gtatgcgtaa attcaaagtc cgggtcaatg gtgaagaatt tgaggttgaa
     1981 gtatctgaag taaatgaatc ccgggaagac gggaagattc gagtcgagaa gataaaaaga
     2041 attcctcatg aaattacagc aacaagcaat cctatgcaag taacaacggt tcttagggct
     2101 cccatgccgg gaagagtctt gtcggtgcaa gtaaaaaagg gtgaacgagt ttcagggggg
     2161 caagttggta taattttaga agcaatgaaa atggaaaatg aaattcaaat tgaaaatgaa
     2221 ggaattgtga cccaaatctt tgttgaggag aacgatactg ttgacaccgg cgatccgcta
     2281 ttagaatgcg aagaaaatcc gagggatatc tcatgaaata ttctaagcta tttctcctca
     2341 tagcaattat atctgctctg cttggcgcaa taatggtgtt acccacaaga ttaagaaacg
     2401 aatcagctat gaaagaaatt gaagttgttt gcgactggca ggatattgaa gtcatatcac
     2461 tccgtttggg aatatcggtt gaggaaacct tggacgaatt gaaacaagca ggtgtgagtg
     2521 ttattggcgt cagtgattat gatttaaaaa atcttcagca attggggaag gtgttaccag
     2581 ttgtcgttga tcatgattat ccatccattc ttcgttattt ttatgtctct aatagtactt
     2641 taaaagaaac tattatccac cacttaaaca ttttaggtcg ggttccagta gaagtttccc
     2701 caaatattat tggcttaaat atttcctatg aagaagagga taagataggt ttgggttggg
     2761 attataacct tgtgcagctt ttaaaagata agggattccg gattgtactt cgtccaagaa
     2821 actggcaagg tatttctcca gaaatcttaa aagctgtcct ttccgatccg atttttgatc
     2881 aagcggatgg tttaattttc tttggtgacc aagtgctggg aaatggaaat gaagaatctt
     2941 tgaaggaaat ggctaacttt cttgatgaga aaaagctatt ttggggatac accgaattcg
     3001 ttggacaaaa aggagaaact atccttgctc gtcaagttcc tggtcaaacc atccgagttc
     3061 atagtatccc accggatgaa attaagaact atactcctct cgaggcccga gaaaggtttc
     3121 tccgagcggt gaaagaacga tcagttcgtc ttctttacgt tcgttttttt actgaaccga
     3181 ctataaacct tggggaaaaa aattattcct atcttactgc gctattttct gacattgagt
     3241 ccagtggatt tacccgtgga actattcacc ccctaatccc ctttgcacct cccttgattg
     3301 cctcagttct attttcagtt tcggtggcaa ttgctattgc gctgctttat agttactttt
     3361 tacccggaaa atggataatt ctcattttaa gcttatttgt attattagga gccctttttc
     3421 aggatcaaat gttgagcctg aggattattg gtgtacttgc tggtattgct tatccaagtt
     3481 tagccgtttt ttccctgatt gatgggcttc ggtcaaaaaa gaatccgttg gtttccatac
     3541 tggtagcttt ttgtatggta tttgccggtg gactggtggt ttcctgtggt ctttatcatt
     3601 ggctttatgt tcttcggatt gagcaatatt caggggtgaa aacctcgttg gtcattccaa
     3661 ttcttttagt tgttctttat ctttttaggt cgggatatct gaatcgtagt attcgttcag
     3721 tggttttagg gactttaaag cgttatgaat ttgttatatt aatgattctc gctggaggat
     3781 ttgtggttta tttgacccgt tcaggtaact ttccattact cccagccgga atgttagaaa
     3841 gcaaaatgag gctttggttg gaaagattgc tctttatgag gccacgaacc aaggaattta
     3901 tgattggtta cccggcttta tggcttctta tttctctccg ccgttttaat tatcaacctc
     3961 tttttaaggt tgtgctttgg cttggtgtaa caattggttt tatcaccttc ttcaattctt
     4021 tttgtcatat ccatacccca tttcttttca tccttctccg attctttaac gcctttatac
     4081 taagtttagt gatattcatt ttttattatc taattgttcg aatcgcctat tggatatgga
     4141 aatggttggg tcaatttgga gaaaatgatg ctcataaaaa aagtacaaat atataaaccg
     4201 ctttaacatg aaagatactc aaaaaaaaat atttttactc ggttattatg gttttggtaa
     4261 ttggggagac gaactttctc ttcaatcaat aatcaatgat ttggaaatta tctctaaaga
     4321 tactccctat tcttttcttt attcggtttt aaatcaagat aatagatttt tccctcgatc
     4381 cttctctaat ctatcagtaa ttattcgaaa aaaaataatt acagttttaa aagaaattac
     4441 caaaagcgat tatgttgtcg taggaggagg gag
//
LOCUS       DAOSOL010000097         4431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_19753_length_4431_cov_3.544790, whole genome shotgun sequence.
ACCESSION   DAOSOL010000097 DAOSOL010000000
VERSION     DAOSOL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19753_length_4431_cov_3.544790"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /gene="nrdR"
                     /locus_tag="PK171_03370"
     CDS             <1..146
                     /gene="nrdR"
                     /locus_tag="PK171_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOT05275.1"
                     /translation="RVGEKVMEKLKELDQVAYVRFASVYREFRDIDQFMEILTTLKTG
                     EKQ"
     gene            247..2349
                     /locus_tag="PK171_03375"
     CDS             247..2349
                     /locus_tag="PK171_03375"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOT05276.1"
                     /translation="MSKSFDVDILTPQKVQKRDGRIVPFDRKRIERAIYKAFQAVGET
                     SETIPNELSQVVTNQLFEKFGTNTTVDIETIQDLVEETLIQYGYSKVSKAYILYRRKR
                     QEAREALQAAVDVEKIVQEYLLKADWRTQENSNTTYSYPGLVLHAAGSVMAHYTLNRI
                     YPDEVRDAHINADVHIHDLSYGITAYCAGWSIEELIREGFGGVKDKVAAGPAKHLSAL
                     TGQMVNFLGTMQMEFAGAQAFNSVDTYLSPFVRFDHLDYRMTKQLIQQMVFAMNVPSR
                     WGSQAPFINFSFDWIVPEDMKERPVIIGGKEQPDLGCYGDYQQEMDMINRAFIEVMLE
                     GDFEGRVFSFPIPTYNITADFPWDSENAMLLWQLTAKYGVPYFQNFISSSLKPGDVRS
                     MCCRLQLDLRVLRNRGGGLFGSADKTGSIGVVTINLPRIGYLSKDEEDFFARLKAKMN
                     IAKTALEIKRKVVERNMKNGLLPYTKRYLGTFKNHFSTIGLVGMNEACINFIGKNIAD
                     PEGKSFALKILKYMRECLADFQEETGNLYNLEATPAEGTSYRLAKHDKKHFPDIYTSG
                     ENEPYYTNSTNLPVNYTDDPFEALDHQKDLQALYTGGTVFHAFMPETPEPETVKLFIK
                     RACEKYTVPYITVTPTFSVCPNHGYISGRIFQCPECGTDTEVYSRVVGYYRPVQRWNH
                     GKQEEFKQRQEYVVSNTI"
     gene            2412..3116
                     /locus_tag="PK171_03380"
     CDS             2412..3116
                     /locus_tag="PK171_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOT05277.1"
                     /translation="MIFRGWQKVSYIEYPGKIATVLFTGGCNFRCPYCHNPELVFLKG
                     SLPQIHESIVLEFLLRRKGMVDAVCITGGEPLIHAPDLFPFLEQVKQKGFLIKIDTNG
                     SLFQPFQQIFQAGLADLWGIDYKLPFSLYKKVLGEKWYKNCQSVLDLALNNPNQAEIR
                     TTIYPPFHDEKILFEMAEKCSSANHWYWQNFHPQKTLSDEARTISPYVPSLLNQWRDR
                     INHQIQKDLIVIRPYS"
     gene            complement(3142..4134)
                     /locus_tag="PK171_03385"
     CDS             complement(3142..4134)
                     /locus_tag="PK171_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05278.1"
                     /translation="MAQRENVSKLRKTMEIVRKTRSIGVIFALIVLVILASLLSPYFL
                     SGYNLQALTRSLAFVGMIALGQACLLLIGELDLSVGAIAGFCGVIGGVLIINLGFHHW
                     LAFGLCLLLGAGCGFLNGALVAKLNLNSLVVTVGMTGVYTGLNLVISKGRAITGIPKA
                     LYFLGQGDFLGIPAPFIIMIGVAGVVLFVTQNTIFGRYMYSVGNNREASKILGIRVER
                     VQIITFMMAGLFAALAGMIMVARLGSSQPAIGQEWVLPSIASSVIGGVSPAGGIGNPV
                     GAVLGAVITGVIENIIVIFGISPYWQTAVSGVVVVAAVSLDSIQRIFYERKVKL"
     gene            complement(4156..>4431)
                     /locus_tag="PK171_03390"
     CDS             complement(4156..>4431)
                     /locus_tag="PK171_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05279.1"
                     /translation="PKVIIFDEPTKGIDVMTKTEIYKIMKDLAEKEKIGIILVSSELE
                     ELMRCCNRLITVYRGKITGEFDPSKVKTSEIIASAINVNDIEQVAAK"
BASE COUNT         1406 a          919 c          810 g         1296 t
ORIGIN      
        1 cccgagtggg agaaaaggtt atggaaaaat taaaggaatt agaccaggtg gcttatgtcc
       61 gttttgcctc agtatatcga gaattccgag atatcgacca gtttatggaa attttaacta
      121 ctttaaagac cggagagaaa caatagttct acaaaccaat gctatttatt aattcagttc
      181 actaattagt tttgttgttt ttttctttta ttatcaattt cttaaaaagt atatggaggg
      241 atttttgtga gtaaatcttt tgatgttgat atattaacac cccaaaaagt gcaaaaaagg
      301 gatggccgaa tcgttccctt tgatcgaaaa agaatagaga gagctattta caaagctttt
      361 caagcggtag gtgaaacatc tgaaacaatc cctaatgagt tgagtcaagt ggttaccaat
      421 cagctattcg aaaaatttgg aacgaacacc actgttgaca ttgaaaccat acaggattta
      481 gtagaagaaa ctctgattca atatggctat tcaaaggttt caaaagccta tattctttat
      541 cggcgcaaaa ggcaggaagc ccgggaagct ctccaagccg cggtggatgt ggaaaaaata
      601 gtccaagaat accttcttaa agccgattgg cgaacccagg aaaattccaa taccacttat
      661 tcttatccag gattagtcct tcatgctgct ggttcagtaa tggcccacta taccctcaat
      721 cgaatttatc ccgatgaagt aagagatgcc cacataaacg ccgatgttca tatccatgac
      781 ctctcttatg gtattacagc ctattgtgca ggttggtcaa tagaagaact tattcgagaa
      841 ggctttggag gtgttaagga taaggttgca gccggtccag ctaaacacct atcagcgctt
      901 actggacaaa tggtaaattt cttgggaacc atgcaaatgg agtttgccgg tgctcaggct
      961 ttcaactccg tagacactta tttatctccc tttgttcgtt ttgaccatct cgattatcgg
     1021 atgaccaaac agctcattca acaaatggtg tttgccatga atgttccctc tcgctggggg
     1081 agccaagcac ctttcatcaa tttctctttt gattggattg ttccagaaga tatgaaagaa
     1141 agaccagtga ttattggtgg taaagaacaa ccggatttag gctgttatgg tgattaccag
     1201 caagaaatgg atatgatcaa tcgtgctttt atcgaagtaa tgttggaagg cgactttgaa
     1261 gggagagttt tttctttccc tataccaact tacaacatta ctgctgattt cccctgggat
     1321 tcagaaaatg ctatgctgct ttggcaatta actgctaaat atggtgtccc atattttcag
     1381 aactttattt ctagttccct gaagccgggt gacgttcgat ccatgtgctg ccgccttcaa
     1441 cttgatttaa gagtccttcg taatcgagga ggaggtttat tcggatcagc ggataaaact
     1501 ggttcaattg gtgttgtcac cataaatctg ccaagaatcg gctatctcag taaggacgag
     1561 gaagattttt ttgcccggtt aaaagcgaag atgaatattg ctaaaactgc tctggaaata
     1621 aaaagaaaag ttgttgaacg gaacatgaaa aacggtcttc ttccttatac aaaaagatac
     1681 ttggggacct tcaaaaatca tttttcaacc attgggttgg tgggcatgaa tgaagcctgt
     1741 attaatttta ttggaaaaaa tattgccgac ccagaaggaa aatcttttgc cttgaaaatt
     1801 ctcaagtata tgcgagaatg tctggcagac ttccaagaag aaactgggaa tctctataat
     1861 ctggaagcta ccccagccga aggcaccagt taccgcttgg caaagcatga caaaaaacat
     1921 tttcccgata tttatacctc aggagaaaat gagccctatt ataccaacag taccaattta
     1981 ccagtaaact atactgatga tccttttgaa gccttagatc atcaaaaaga cttgcaggct
     2041 ttatatacgg gtggtacggt atttcacgct tttatgcccg aaacgcccga gcccgagact
     2101 gtcaagctct ttataaagcg agcttgtgag aaatatactg ttccctatat taccgttaca
     2161 ccaacttttt cggtctgtcc aaatcatgga tatatttccg gtcgcatctt ccaatgtccg
     2221 gaatgtggaa ctgatactga ggtatactct cgggtagtag gatattatcg gccggttcaa
     2281 cgttggaacc acggaaagca agaagaattt aaacaacgcc aagaatatgt ggtttcaaat
     2341 actatttgaa agtttagtgg tttagaattc catgatcgaa tctgaataaa tttattcaat
     2401 acaaagaaat tatgattttt cgtggctggc aaaaagttag ttatatcgaa tacccaggga
     2461 agatagctac cgtcttattt actggcggtt gtaattttcg ttgtccctat tgccacaatc
     2521 cagaattagt atttttaaaa ggatccctcc ctcagattca tgaaagtatt gttcttgagt
     2581 tcctgctgag aagaaaaggg atggttgatg cagtttgtat caccgggggt gaaccgttaa
     2641 tccacgcccc cgatcttttt ccctttttgg aacaagttaa gcaaaaggga tttttaatca
     2701 aaatcgatac caatggaagt ctttttcagc cattccagca aatttttcaa gctggtttgg
     2761 ctgacctgtg gggaatcgac tataaacttc ctttttctct atataaaaag gttttaggag
     2821 aaaaatggta taaaaactgt cagtcggttt tagacctagc gcttaataat ccaaatcaag
     2881 ccgaaatccg taccaccatt tatccacctt ttcacgatga aaaaatcctt tttgaaatgg
     2941 ctgaaaagtg ctcctccgct aatcattggt attggcaaaa ctttcatcct caaaaaaccc
     3001 taagcgatga ggctcgtact atttctccat atgtaccctc acttctcaac cagtggcgag
     3061 accgaattaa tcaccaaatc cagaaagacc taattgtcat tcggccttat tcttgaacac
     3121 atttctacca ataaaaaagg tttataattt tacttttctt tcataaaaaa ttctttgaat
     3181 tgaatcaaga gatacggctg caacgaccac cactccgctt actgcagttt gccaataggg
     3241 agaaatacca aaaattacaa ttatattttc aataactccg gttatcacgg cacctaatac
     3301 tgcaccaaca ggatttccta tgcctcccgc tggtgaaacc cctccaataa ccgaagaagc
     3361 aattgatggt aatacccact cttgcccaat tgcaggttgg gaggagccaa gacgcgccac
     3421 cattatcata ccagctaagg cagcaaacaa tccagccatc atgaaagtta ttatctggac
     3481 tcgttctacc ctgatcccaa gtatttttga tgcttcacga ttatttccca cagaatacat
     3541 ataacgacca aaaatggtat tttgtgtaac aaataatacc acccctgcaa caccaatcat
     3601 aatgataaaa ggtgcaggaa tccctaaaaa atcaccttgg cctaagaaat aaagagcttt
     3661 cggaatgccg gttatcgctc ttcctttaga aataaccagg ttgagaccgg tataaacacc
     3721 agtcatccca accgtaacga ccaaagaatt caaattcaat tttgcaacta aagctccatt
     3781 aagaaatcca catcccgctc ccaataagag acataaacca aaggcaagcc aatgatgaaa
     3841 ccctaagtta atgatgagca ctccaccaat gaccccgcaa aaacctgcta tagcgccaac
     3901 tgacaaatcc aattctccaa taagcaataa acatgcttga cccaaggcta tcattccgac
     3961 aaatgctaga gaccttgtta aagcctgtaa attataacca cttaaaaaat aaggcgacaa
     4021 aagtgaagca agaataacaa gaacaataag cgcaaaaata acaccaattg aacgtgtttt
     4081 tctaactatt tccatcgttt ttcttaactt cgaaacattc tctctctgtg ccatttcaaa
     4141 cactccaaaa ctatattatt tagcagcaac ttgctctata tcattaacat tgattgctga
     4201 tgcaataatt tctgaggttt taaccttact tgggtcgaat tcaccagtta tttttcctcg
     4261 ataaacagta ataagccgat tacaacatct cattaattct tcaagttctg aagatactaa
     4321 aattattcca atcttttctt tttcagcaag atctttcata attttatata tttcggtttt
     4381 cgtcatgaca tcaattccct tggttggttc atcaaaaatg attacttttg g
//
LOCUS       DAOSOL010000098         4386 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_20005_length_4386_cov_4.120296, whole genome shotgun sequence.
ACCESSION   DAOSOL010000098 DAOSOL010000000
VERSION     DAOSOL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20005_length_4386_cov_4.120296"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK171_03395"
     CDS             <1..411
                     /locus_tag="PK171_03395"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05280.1"
                     /translation="PIVNLQGKVLGHHHGTYLYTIGQRKGLGISHSRPLYVVDIDLSR
                     NAVIVGDKKDLQAMALVATQVNFLVDEIPDQEIFAVTRYSQKEHQCTLEKINHEIKVI
                     FYDKLENITPGQSVVWYTREGIVIGGGIIKEVLR"
     gene            408..1319
                     /gene="nadA"
                     /locus_tag="PK171_03400"
     CDS             408..1319
                     /gene="nadA"
                     /locus_tag="PK171_03400"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOT05281.1"
                     /translation="MNAVIQQIQELKKMKNAIILVHNYQPPDIQDIADFLGDSLGLSI
                     EASQTDADIIVFCGVRFMAETAKILSPNKVVLLPEKNAGCPMADTITVQEVKKLKSVH
                     PKATVLAYVNTTADVKAECDICCTSANALKIVTDGLKDAKEIIFIPDKNLANYISRKT
                     GRQFILWDGDCPIHAHILPEHIHFQKKLHPDALVVVHPECLPEVIDLADEVQSTEGMS
                     RFIGQSKKSEFIIGTEVSILYRLRKENPEKNFYPASAQAICPDMKKITLEKVLYSLRF
                     GYGEINLPETIRQRAERSIQRMLEYKS"
     gene            complement(1340..1699)
                     /locus_tag="PK171_03405"
     CDS             complement(1340..1699)
                     /locus_tag="PK171_03405"
                     /inference="COORDINATES: protein motif:HMM:NF018088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOT05282.1"
                     /translation="MLLRRLEDCDEFIAGDLTRLREILHPDKLGIDIHYSLAHATLPP
                     GRTSQPHYLYSSEVYYIISGKGIMHINSQIEQVEEGSTIYIPPQSIQFIENAGSQDLV
                     FLCIVDPAWKKEDEIVL"
     gene            complement(1719..3212)
                     /locus_tag="PK171_03410"
     CDS             complement(1719..3212)
                     /locus_tag="PK171_03410"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOT05283.1"
                     /translation="MARYIMFQGTGSGVGKSLITAGFCRIFARKGIRVAPFKAQNMSL
                     NSGVTPFGEEIGRAQLLQARAAMIDPDSRMNPILLKPQGDCMSQVIVRGKIWETHEAY
                     DYYRYKKFLWEKVIESLNSLAEEYDLICIEGAGSPAEINLREQDLVNMSVARYARIPV
                     FLIGDIEKGGVFAQLYGTWALLTKEERELLCGFIINKFRGNYTILEPGLKEIEKLTQI
                     PVVGVVPYSRFLLDDEDSMTEKIENSYKSLKTVDIQVGIIRLPHISNFTDFDPLTNEP
                     DVETQYIFPDENFSKFDVIVLPGSKNTIRDLQWLKKFNFQERLESYINNGGVVLGVCG
                     GYQMLGESIIDIDGNEDFQGEIDGLKIIPMKTFFEKEKILKNMQGYLAKAEHISVKGY
                     EIHQGKGSFQQAYEPLFNLNLGNRIEPEGIIINNQIFGTYLHGLFDDGSFRRYFVNLL
                     RKRKGLATISSVSPSWQEVVEKELDRLADFLETCLDIERIESLIENF"
     gene            3658..>4386
                     /locus_tag="PK171_03415"
     CDS             3658..>4386
                     /locus_tag="PK171_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05284.1"
                     /translation="MRGKKKHQNQVVLYIVLGLSISILIAIMTCCEGTIGTSCTDNTK
                     FCAIIKELAPSAVQPALEDCPTSLTICDGDQIELYWKADASLTSNVHITGPGGESFDF
                     PISEGHATVTPRTSGDWKVEFTGGGCTFNKSINVRVIKGEEPYTIVANGNIDIGFFCD
                     INPKSVSRNLIVAAIRSAQCAGVSEYWENWGCKKSNWDGSKPIYFNITSDDGSANFTP
                     LAGRWRFDPSGLGGTYTYSAKSACF"
BASE COUNT         1282 a          929 c          816 g         1359 t
ORIGIN      
        1 ccaattgtta atctccaagg gaaagtactt ggacaccacc atggtacata cctctatact
       61 attggccaaa gaaaggggtt aggaatcagc cattcacgtc ctctctacgt tgtcgatatc
      121 gatctttctc ggaatgccgt aatagttggt gataaaaaag atttacaagc catggctctg
      181 gttgctactc aggtgaattt tttagtcgat gaaatccctg accaagaaat ttttgctgtt
      241 acgcgttatt cccagaaaga gcatcagtgt acattagaaa aaattaacca tgaaataaag
      301 gtcatttttt atgataaatt ggaaaatata acccctggcc aatcagttgt atggtacact
      361 cgtgaaggaa tcgtaatcgg aggaggaatc attaaggagg ttttgagatg aatgcggtca
      421 tccaacaaat tcaagaatta aaaaaaatga aaaatgcgat tattttagtt cacaactatc
      481 aacccccgga catacaggat attgcggatt ttttagggga ttctttgggt ttgagcatcg
      541 aagccagcca aactgatgcc gatatcatcg ttttttgtgg tgttcgtttt atggcagaaa
      601 ccgccaaaat tctctctccg aataaagttg tgctccttcc cgagaaaaat gctggttgcc
      661 ccatggcaga tactattacc gttcaagaag ttaaaaagct taaatcagta catcccaaag
      721 ctacggtgct tgcctatgtg aatacaactg cagacgttaa ggctgaatgt gacatttgct
      781 gtacatcggc caacgcccta aagatcgtta ccgatgggtt aaaagatgcc aaagaaatta
      841 tttttattcc tgataaaaac ttagccaatt atatttcgag aaaaactggt cgtcaattta
      901 ttctctggga tggcgactgt ccaattcatg cgcatattct tcctgaacat atccattttc
      961 aaaaaaaact ccatcccgat gcgttggttg ttgttcaccc tgaatgtctt cctgaagtga
     1021 ttgatttagc tgatgaggtt caatcaactg agggaatgtc gcgatttatt ggtcaatcca
     1081 aaaaaagcga atttatcatt gggactgagg ttagtatact ctatcgtctt cgtaaagaaa
     1141 atcctgagaa aaatttttat cccgcttcag cacaggcaat ctgtcccgac atgaaaaaaa
     1201 taaccctgga aaaggtcctc tattctctcc gttttggtta tggtgaaatt aacctgcccg
     1261 agacaattcg ccagcgagcc gaacgaagta ttcagcgtat gttagagtat aaatcttaac
     1321 tcaagcttat ctctcataat caaagaacaa tttcatcttc ttttttccag gctggatcaa
     1381 caatacatag aaataccaag tcttggctcc cggcgttttc aatgaattgt atggattgtg
     1441 gaggaatgta aatggtcgaa ccttcctcca cctgctcgat ttggctattt atgtgcataa
     1501 tccctttgcc agatataata taatatactt cagatgaata gagataatga ggctgagatg
     1561 tacgcccggg cggaagagta gcatgggcaa ggctgtaatg gatatcaatt cctaatttat
     1621 ccggatggag tatttcacga agtctagtta aatcgccagc tataaattca tcacagtctt
     1681 ccagtcttcg gagtaacata aagatcctcc tgttgtattt aaaaattctc tatcaagctt
     1741 tctatccgtt caatatcgag gcaggtttca agaaagtctg ccaaacggtc aagctctttt
     1801 tctacgactt cttgccagga tggagaaacc gatgatatgg ttgctaaacc ttttcttttg
     1861 cgaagtaagt tgacaaaata tcttcggaac gatccatcat caaacaatcc atgaaggtag
     1921 gttccaaata tctgattatt gattattata ccttcaggtt cgattctgtt ccctaagttg
     1981 agattaaaaa gtggttcata agcctgttgg aaagagcctt tcccctgatg aatttcatac
     2041 cctttcactg aaatgtgttc agcttttgcc aaatatcctt gcatgttttt aagtatcttt
     2101 tctttctcaa aaaaggtttt catcggaatg atttttagac catctatttc accttggaaa
     2161 tcttcatttc catcgatatc tattatagat tctcctagca tctgataacc accacacact
     2221 cctaatacca ctccaccatt gtttatatag ctttccaaac gttcttgaaa gttaaatttc
     2281 ttcaaccatt gaaggtctcg aatagtattt ttgcttccag gaagcactat cacatcaaac
     2341 ttactaaaat tttcatcggg aaaaatgtat tgagtttcca cgtcaggctc attcgttaat
     2401 gggtcaaaat cggtgaaatt tgatatatgg ggaaggcgaa taataccaac ttgaatatca
     2461 acggttttga gtgacttata tgagttttca attttttccg tcatactatc ttcatcatcc
     2521 agcagaaaac gagaataagg aacaacacct acaactggaa tttgagttaa tttttcaatt
     2581 tctttcaatc ctggttctaa aattgtataa ttccctctga atttattaat aataaagcca
     2641 cagagtaatt ctctttcttc tttagtcaaa agtgcccagg ttccataaag ttgtgcaaat
     2701 accccacctt tttcaatatc accaatgaga aatactggta tacgcgcata tcgagctacc
     2761 gacatattga ccaggtcctg ttctcgcaaa tttatctctg ctggactacc agctccttca
     2821 atacagatga ggtcatattc ctcagccagt gaattcaggc tttcgataac cttctcccag
     2881 agaaattttt tataccgata ataatcataa gcttcgtgag tctcccagat ttttccacga
     2941 acaatcacct ggctcatgca gtctccctgg ggtttcagta aaattgggtt cattcgggaa
     3001 tcaggatcaa tcatggctgc tcgagcttgc aacagctggg ctctgccgat ctcctctccg
     3061 aatggtgtca ctcctgagtt taaagacata ttttgtgctt taaacggagc aactctaata
     3121 cccttacgag caaatattcg gcaaaaacct gctgtaataa ggcttttccc aacaccagat
     3181 ccagttcctt gaaacatgat atagcgtgcc ataaaccact ccttgaaaat atactgtttc
     3241 aaattttatc atagggaaga aaatcttagt ctatcctgaa aatatcatct aatgaattcc
     3301 cctccttgga ggggtgccgc tttcgcggcg gggaggggct ttttaattcg tcatcctgga
     3361 ccctcttccc ttcttctttt tctccccctc accccctcgc gccctctccc cctcagaatc
     3421 taatggcgtg aggatttcat ctttttcttt tctcgcactc tctcccgttc tccctctcgc
     3481 cccttggtgg gagagggaga acggttaata aattaactaa atttttttaa atttttggta
     3541 tctaatagag tataataata atcagtcaac tatcccggca aacaaacact tcctatcacg
     3601 cccattctac ctaaaaaacg ctgttgattt tttaattttt tttaggaggg aaaaccgatg
     3661 aggggaaaga aaaaacatca aaaccaagtt gttctctata tcgttttagg attgtcaatt
     3721 agtatattaa tagcaattat gacttgttgt gagggtacaa tcggtacatc ttgtactgat
     3781 aataccaagt tttgcgcaat tattaaagaa ctggcacctt ctgcggtaca acccgccctg
     3841 gaagattgtc caacttcatt aaccatctgt gatggggacc agattgaact ctactggaaa
     3901 gccgatgctt cattaaccag taatgtccac atcactggtc cgggtgggga aagttttgat
     3961 ttccccattt ccgaagggca tgctactgtt acacctcgaa cctcgggaga ctggaaagtt
     4021 gaatttactg gaggcggttg tacttttaat aaatcaatta acgttcgtgt tatcaaaggc
     4081 gaagaacctt ataccattgt ggctaatggc aatatcgata ttggattttt ctgtgacatc
     4141 aaccccaaaa gcgtatccag gaatttaatt gtagcagcaa tacggtcagc tcagtgtgct
     4201 ggcgtctcag aatattggga aaattggggc tgtaagaaat ccaattggga tggctccaaa
     4261 ccaatctatt ttaatatcac cagtgatgat ggatcagcca acttcacacc acttgctgga
     4321 cgttggcgtt ttgatccttc tggtttagga ggaacctata cctattcagc aaaatctgcc
     4381 tgtttc
//
LOCUS       DAOSOL010000099         4334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_20332_length_4334_cov_3.894835, whole genome shotgun sequence.
ACCESSION   DAOSOL010000099 DAOSOL010000000
VERSION     DAOSOL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20332_length_4334_cov_3.894835"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3459)
                     /gene="rpoB"
                     /locus_tag="PK171_03420"
     CDS             complement(<1..3459)
                     /gene="rpoB"
                     /locus_tag="PK171_03420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT05285.1"
                     /translation="MNSGYIQRKTFSKVTSFAELPHFIEIQRKSFDLFLQANISPQER
                     KRQGLQEVFTEIFPIQDFTGKLMLEFIDYRLDRPVFSVRECKDRGRTYAAPLFARVRL
                     INRETNEIKEQDVYMGEIPLMTDKGTFVVNGAERVVVTQLIRSPGIFFTKEVTPTGKV
                     EYGFKIIPNRGAWLEFGIDPGEILFVRVDKRRKINAMTLIRALGFSNDEEILELFDNH
                     SYIQATLERETTSNPKEAVMEIFRRLRPSEPPTEENTRDFMRYLFFDPRRYDLGEVGR
                     YKLNNKLKLEERIVRIEEFVGAEDIILDHEEHQCLRNQAAIDENNPSRYEILLHKGDK
                     IGRQLARTLEELNQEYNIEFIRVYNDEDQVIKVFIEREDELELVGDELEGRSISQDVL
                     QPETGETLLKKGDMVTLEALRTLRVHGIEKIWVEKGRILSQADVVGGIRYLLNLLNGV
                     GYDDDIDHLGNRRARPVGELLQNQFRTGLLRVEKVIREKMTIQPDTDSATPQNLINVR
                     PVVAAIKEFLGSSPLSQFMDQINPLSEITHKRRLSALGPGGLSRERAGFEVRDVHYTH
                     YGRMCPIETPEGPNIGLITSLCIYAKINEYGFIVTPYLKVENRHLTDEIRYLSADEED
                     RYHIAPSDAVIDENGMIRDENVVVRFRSKIVEVESRKVDFIDVSPQQIISASASLIPF
                     LEHNDANRALMGTNMQRQAVPLLVSKPPLIGTGMEYKVAQDSGATVVSRNSGVVKQVD
                     SVFIEIENDDGDIDLYELEKFQRSNQSTCINQKPIVRVGQYVEAGQIIADGPCTSMGE
                     IALGRNVLVAFMPWEGENFEDAILISERLVKEEVFTSVHIEEYEIEARDTKLGPEEIT
                     RDIPNLGDDILRNLDDEGIIRVGADVKPGDILVGKVTPKGETELTPEERLLRAIFGEK
                     AREVRDTSLRVPHGEYGKVIDVKVFSRENDDELAPGVNKLVKVFVAQKRKITVGDKMA
                     GRHGNKGVIARILAEEDMPFLPDGTPVDIVLNPLGVPSRMNIGQILETHLGWVANKER
                     MFIASAPFDGAKESEILEALKDVTISQENRYPVFAFDDRISPEFNLLPHGKVILFDGR
                     TGEPFDNEVTVGYIYMMKLAHLVETKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEME
                     VWALEGY"
BASE COUNT         1221 a          992 c          790 g         1331 t
ORIGIN      
        1 gtatccttcc agtgcccata cttccatttc tccaaaacgc tgcccgccaa attgggcttt
       61 acctcccaac ggctgttggg taactaaaga gtacggtcca gttgaacgtg catgaatctt
      121 ggtttcaacc aggtgagcaa gtttcatcat atatatgtat ccaactgtca cttcattgtc
      181 gaaaggttca ccagttcttc catcgaaaag gatcactttt ccatggggga gaagattaaa
      241 ctccggcgat atcctgtcgt caaaagcaaa aaccggataa cggttttctt gactgatggt
      301 tacatctttc aaggcttcta aaatctccga ttctttcgct ccatcgaaag gagcactggc
      361 aataaacatc cgttctttat tggcaaccca acccaagtga gtctctaaaa tctgcccaat
      421 attcattcga gaaggaactc ccagtggatt gagaacaatg tcaaccggtg ttccatcggg
      481 aaggaagggc atgtcctcct cagcaagaat cctggctata actcctttat ttccatgacg
      541 accagccatt ttatcgccaa cggtaatttt tcttttctgg gcgacaaaca ccttaaccag
      601 tttattgact cctggtgcca gttcatcatc gttttctcga gaaaatactt taacatcaat
      661 aactttccca tattctccat ggggaacccg aagggatgta tctcgaactt ctcgggcttt
      721 ttctccaaat attgcccgaa gtaatcgctc ttcgggggta agctcggttt ctcccttggg
      781 agtaaccttc cctaccaaga tatctcctgg ttttacatca gctccaactc ggataattcc
      841 ttcatcgtct agattccgaa gaatatcatc tcctaaattg ggaatgtcac gagtaatttc
      901 ctctggacct aacttggtgt ctcgggcttc gatttcatat tcttcaatat gaactgacgt
      961 gaaaacttct tctttaacca atcgttcgct aatcagaatg gcatcttcga agttctctcc
     1021 ttcccagggc atgaaagcta ctaagacatt tctccctaaa gcaatctcac ccattgaagt
     1081 acaaggccca tcagcaataa tctgtccagc ctcaacatac tgcccaactc gaacgatcgg
     1141 tttttggttg atacaggtac tttggttgga ccgctgaaat ttttccaatt catagagatc
     1201 aatatcacca tcatcatttt caatttcaat aaaaactgaa tcgacttgct taactactcc
     1261 actatttcgg ctaacaactg tagcacctga atcctgagcg actttgtatt ccatgcccgt
     1321 cccaataaga gggggcttgg aaaccaaaag aggaaccgct tgtcgttgca tgttggttcc
     1381 catcagagct cggttggcat cattgtgctc taaaaatgga atgagtgacg ctgaagcact
     1441 gatgatctgt tgaggcgaaa catcaataaa atcaactttt ctgctttcta cctcaacaat
     1501 tttcgaacgg aagcgaacta cgacgttttc atcgcgtatc atcccgttct catcgataac
     1561 agcatcggaa ggcgcaatat gataacggtc ttcttcatca gcagaaagat acctaatttc
     1621 atcggtcaaa tgtcgatttt caacttttaa atagggtgtc acaataaagc catactcatt
     1681 gatcttggca tagatacata gagaggtaat taagccaatg ttgggtcctt cgggagtttc
     1741 tatcggacac atccgcccat aatgggtata atggacatca cgaacttcaa aacctgctcg
     1801 ttcacggctc aaacctccag gtcctaaagc acttaaccgt cttttatggg ttatttccga
     1861 aagtggattg atttgatcca taaactggga aagaggactg cttcctaaaa attccttgat
     1921 agcggctacc actggacgaa cattaatcag gttttgagga gtagctgaat cggtgtcggg
     1981 ttggatggtc attttttctc gaatgacctt ttcaactcgc agcaacccag ttctaaattg
     2041 attttgtagg agctctccca ccggtcgagc tcgtcgattt cccaggtggt caatgtcatc
     2101 atcatagcca acaccgttca acaagttgag gagatagcga atgcccccaa ctacatcggc
     2161 ttgactcaaa atccgtccct tttccaccca aattttttct ataccatgaa cccttagggt
     2221 ccttaaggcc tctaaggtca ccatatcgcc ttttttaagg agggtttctc cggtctccgg
     2281 ttgaagcaca tcctgactta tcgatctgcc ctccaactcg tcgccaacca actccaattc
     2341 atcttcgcgt tcaataaata cttttatgac ctgatcttcg tcgttataca ctcgtataaa
     2401 ttcgatattg tattcctggt taagctcttc taaggttcga gctaattgcc gaccaatttt
     2461 gtctccttta tgaaggagaa tttcatatcg ggaaggatta ttttcatcga tagctgcttg
     2521 attgcgaaga cattgatgtt cttcatggtc aagaataata tcctcagctc caacaaactc
     2581 ttcaattcgg acaatgcgtt cttccaattt tagtttatta ttcagtttat atcgtcccac
     2641 ctcaccaagg tcataacgac ggggatcaaa gaacaaatat ctcataaaat ctcgagtatt
     2701 ttcttcggtt ggtggctcac tgggacgaag tcgccggaaa atttccatga ccgcttcttt
     2761 ggggtttgaa gttgtttctc tttccagggt ggcttggatg taagaatgat tatcgaacag
     2821 ttcgaggatt tcctcgtcgt ttgaaaatcc caaagctctg atcaaagtca tagcattgat
     2881 cttgcgcctt ttatcgacac gaacaaagag aatttctcct gggtcaattc caaattctaa
     2941 ccaggcacca cggttgggaa tgatcttaaa accatattcc acttttccag ttggtgtcac
     3001 ttctttagta aaaaatatcc ctggagatcg gatgagctgc gttactacaa cccgttccgc
     3061 tccattcacc acaaaagtcc ccttatcagt catgagtgga atttcaccca tatatacatc
     3121 ttgttctttt atttcattgg tttcgcggtt aatcaatcga actcgagcaa ataatggggc
     3181 agcatatgtc cgacctctat ctttacattc acgaaccgag aatactggcc tatccaaacg
     3241 ataatcgata aactccagca tgagtttccc ggtaaaatcc tgtataggaa aaatttcggt
     3301 gaaaacttct tgcaaacctt gccttttccg ttcttggggg gaaatattcg cttgtaaaaa
     3361 taggtcaaat gactttcttt gtatctcaat aaaatgggga agttcggcaa atgaagtcac
     3421 ctttgaaaat gtctttctct gaatgtatcc agaattcacc cagcacacca cctttgttga
     3481 aaattaaaag aaattactct ttaaactaat cagtttttca tcatcattaa actgtggcga
     3541 aacggtgtaa gtttaaaatg tggaatgtat gaaattcaca atgtacaggt aaaaaaaata
     3601 agaggacaaa tggattgatt ttatcatcga ttggtaagat aataataaca cccacttagt
     3661 aataatttgg aacaaaatag attaatttag ttccaaaatg aaagaaccga caatttacta
     3721 taatatttta ttcctgctta tctgtcaaga gcttcttgta aatttttcta aaatatttta
     3781 aattctccat tccttggtct actttatgaa agaagtcagc ttcccttttt tctctctcct
     3841 ggtgagagaa cttcatcctt tttttcttct cccctggtgg gagagcttct ttcttttctt
     3901 tttttcctct cccctggtgg gagaggatta aggtgagggg gatgctttgg tatttacatc
     3961 aaactttaat ttttaaaacc aaacatgaat cagagtcctg actttcaccc tcatcccaac
     4021 cttcttttat caaggcgaaa agtggttgta tatatgcgtc atcctaagcc ctcgcttttt
     4081 gagagcgtga ggatctcctc ttttatgtct ttgttcttca taattattta aaaagatgag
     4141 attctcaagt cgcacaatac gctcctcaga atgacaaatt gagtaaatga gattgcgaag
     4201 tcgttaaacc aaaagacgat tagactcctt acaataatag atttggctaa cgggattctc
     4261 acgtcgtcag ttaaaaacac cggattcctc agaatatcaa actaaaataa aagactccac
     4321 aactactgag gaaa
//
LOCUS       DAOSOL010000100         4329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_20358_length_4329_cov_4.090314, whole genome shotgun sequence.
ACCESSION   DAOSOL010000100 DAOSOL010000000
VERSION     DAOSOL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20358_length_4329_cov_4.090314"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /gene="glgD"
                     /locus_tag="PK171_03425"
     CDS             <1..1236
                     /gene="glgD"
                     /locus_tag="PK171_03425"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOT05286.1"
                     /translation="ILAGGKGNHLSVLSIERAKAAVPFGGFYRLIDFPLSNCINSGIY
                     DVGILTQYQPRSLIEHIGVGRPWDMDRKKGGIELLQPYLGKTVGGWYRGTGDAVYQNL
                     NIIKRKKLDHLLVLSGDHAYMMDYNSLMDYHLDNDADVTLVVTRVKTEDRSRFGILEI
                     DGKNQVIGFEEKPSQPKTNIAFMGIYIFRLEFLNECLQKNAQESKYDLVNNVIIENLN
                     SIKIQAFFFDGCWFDTGTIKAYWEANMHLLEPLPCFNLYDPNWVIYTNRSQNPPAKIG
                     KGALVQSSIIGEGTVIHGEVIHSVVFPGAVIEKNVKIYDSIIFNDSIIKAGSVVERSI
                     LDKNVIVGEDSIIGAGDDYTPNRLRPDILSWGVNLVGKNSQIPLHSIIPRNCLIGINV
                     PTSRFGKITELKSGSVIMG"
     gene            1332..1535
                     /locus_tag="PK171_03430"
     CDS             1332..1535
                     /locus_tag="PK171_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05287.1"
                     /translation="MNERLKSISLMIHKNNENLKKMEEKFSLLLSLTEERGSKKTSVD
                     VEDSFCFDVFEDQKDESDSKGDI"
     gene            1532..2500
                     /locus_tag="PK171_03435"
     CDS             1532..2500
                     /locus_tag="PK171_03435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT05288.1"
                     /translation="MMKRRYSVFIVSIIFMIGIFLFSIQSIAQENSSAEGAVEEAQNI
                     ETPVTNEQVVIAEVNGEPVYLAELKEIWDTMPENYRTQFPGGFKDLLEQWIRQVLLVQ
                     EAKKLGLADDPDVKKKIENVTQQIMIQEFVTREIIEKAVVSDEEIEKEYSSNPTLYTE
                     AEQVKARHIMVNTKEEADVVQKELKEGKPFEQVAKEKSQSPDAQTGGEMGMIRKGDLE
                     PEMEKVLFDLAPGNVSESIETDYGIHVFLVEDHLQPRLKDLAEVKEEIRTKLLPKVQQ
                     EAFEKMIEAMKNKSEIAILEENLPKEEIPEAPATDTNPETATPPTE"
     gene            2719..>4329
                     /gene="ileS"
                     /locus_tag="PK171_03440"
     CDS             2719..>4329
                     /gene="ileS"
                     /locus_tag="PK171_03440"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT05289.1"
                     /translation="MFESLGKVSELGYREEKILDFWREKKIFQKSYQQREGNQRFIFY
                     EGPPTTNGYPHAGHIIGRSMKDLIPRYKTMCGYYVPRKAGWDTHGLPVELEVEKMLGF
                     SGKQDIENYGIDRFNQKCRESIWKYIKEWEKITERMGIWMDMDHPYVTCDNDYIESLW
                     WILKQLFNQNLLYQGYKVLPYCSRCGTSLSSHEVAQGYQDVVDPSIYLLFQIQNRENT
                     FFLVWTTTPWTLVANVALAVGQDLDYVEIEEKETGKHLILNRESLPALFQPDAYTLIR
                     EMKGKELIGLSYNPLMTFLAAPKGYQVYHGDFVSTEEGTGIVHIAPAFGEDDMRLAQT
                     FDLPVLHPVKADGTYDESVPLWHGLWVKDADPLIIKYLQSTGQLYRQETHRHTYPFCW
                     RCDTPLLYYAKGSWFIRSTAVKETLLENNLKVHWHPDHIQSGRFGNFLENVVDWALSR
                     ERYWGTPLNIWKCESCGYQEAIGSRQELIQRANQQIDQIELHRPYVDAITLTCSQCGG
                     EMRRTPEVLDCWFDSGAMFVAQNHYPFEN"
BASE COUNT         1390 a          716 c          953 g         1270 t
ORIGIN      
        1 attttagccg gggggaaagg caatcacttg agtgttttat caatagaaag agccaaagca
       61 gctgttccct tcggaggttt ttatcgatta attgattttc ccttaagtaa ctgtattaat
      121 tcaggtatct atgacgttgg tattttgacc cagtaccagc cgcgttcgtt gatagagcac
      181 attggggttg ggcgtccctg ggacatggac cgaaaaaagg gtggaataga gctccttcaa
      241 ccttatttag gaaaaaccgt tggaggttgg tatcgtggaa ctggagacgc tgtttatcag
      301 aatttgaata ttattaagag aaaaaagttg gatcatcttt tagtgctttc cggtgaccac
      361 gcctatatga tggattataa ttctcttatg gattatcacc tggataatga tgccgacgtt
      421 accttagtag tgacccgggt gaaaactgaa gatcggtcac ggtttggaat tttagaaatc
      481 gacggtaaga accaagttat tggttttgaa gaaaaaccat cacagccaaa aaccaatatt
      541 gctttcatgg gaatttatat ttttcgttta gaatttttaa atgaatgttt acaaaaaaat
      601 gcccaagaat cgaaatatga tttagtcaac aatgttatta ttgaaaattt aaattctatt
      661 aaaattcaag cttttttctt tgatggttgt tggtttgata ctggaaccat aaaggcgtat
      721 tgggaagcaa atatgcattt attagagcct cttccctgtt ttaacttata tgatccgaat
      781 tgggtaattt ataccaatcg atctcaaaat cctcctgcca agattggaaa gggggctttg
      841 gtccaatcat cgataattgg tgaggggact gttatccatg gcgaggtgat ccattcagtt
      901 gtttttcctg gagcagtaat tgaaaaaaat gtgaaaattt acgattcgat aatatttaat
      961 gattcaataa ttaaagctgg ttcagtggtg gagagaagta tactggataa aaatgttatt
     1021 gtgggggagg attcgattat aggagcagga gatgattaca ctcccaaccg acttcgaccg
     1081 gatatactga gttggggtgt taatttggta ggtaaaaatt ctcaaattcc tcttcactcc
     1141 ataattcccc gaaattgttt aattggtatt aatgtcccca ccagtcgttt tggaaaaatt
     1201 actgagctga aaagtgggtc agtaattatg ggatagcgtg agaaaagaaa tcttggaggg
     1261 aggttagttc tttgatcctt ggactcttga ttataattct cattttgtat gggctcgtat
     1321 tattttgggt tttgaatgag cgtctgaaaa gcatttcatt gatgatacat aaaaataatg
     1381 aaaatttaaa aaaaatggaa gaaaaatttt ctttgttgtt gagtttgact gaggaaaggg
     1441 ggagtaagaa aacatcagta gatgtagaag attctttttg ttttgatgta tttgaagatc
     1501 aaaaggatga atcagattcg aaaggagata tatgatgaaa cgaagatatt cagtttttat
     1561 agtaagtatc atttttatga ttggtatttt tttgttttct atccaaagca tagctcaaga
     1621 aaattcaagt gctgaaggtg ccgttgaaga agcacagaat atcgaaacac cagttacgaa
     1681 cgaacaagtc gtcattgctg aagtcaatgg agaaccggtt tatttagctg agttaaaaga
     1741 aatttgggat accatgccag aaaattatcg aacccagttt cctggtggat ttaaagattt
     1801 attagaacaa tggatacgtc aagttctttt ggtccaagaa gcgaaaaaac ttggtttggc
     1861 cgatgatcca gatgtgaaga aaaaaattga aaatgtaacc cagcagatca tgattcaaga
     1921 atttgtgact cgagaaataa ttgaaaaggc tgtggtttct gatgaagaga tcgaaaaaga
     1981 atattcatca aatccgaccc tttataccga agcagaacag gtaaaagcca ggcatatcat
     2041 ggtgaatacg aaagaagaag ccgatgtagt tcaaaaggaa ttgaaagaag ggaaaccttt
     2101 tgagcaagta gccaaagaga aatctcagtc tcctgatgcc caaactggcg gagagatggg
     2161 tatgatacgc aaaggcgatc ttgagccaga gatggaaaag gttttatttg atctggctcc
     2221 tggcaatgtg agcgaatcaa tcgaaaccga ttatggtatt catgttttct tagttgaaga
     2281 tcatttgcag cctcggttga aagaccttgc tgaagtaaaa gaagaaatcc gtaccaagct
     2341 gcttcccaaa gttcagcaag aggcatttga aaaaatgatc gaagctatga aaaataaatc
     2401 agaaattgcc attcttgaag aaaaccttcc taaagaggaa atacccgaag ctccggcaac
     2461 tgataccaac ccagaaacag ccacccctcc aactgaataa taaattaaag gatgggattc
     2521 ctccaaagaa gtgctttaca acagcgaatc ccatccagta aaaaagtaac catttttaca
     2581 ggcagcgata aacccctaat aaattgcaaa aaatcctgaa ttcggttata ttgtttaagt
     2641 atttcttgga tagagataaa ataagaaagg gtattttcga actatctttc cagctcatca
     2701 ttgcaggcag gtgacaatat gtttgaatca ttagggaaag tctctgagtt aggttatcgc
     2761 gaagaaaaga tacttgattt ttggcgagaa aaaaagatat tccagaaaag ttatcagcaa
     2821 cgagaaggaa accaacgttt tattttctat gaagggccgc caaccaccaa tggatatcct
     2881 catgccggcc atattattgg caggagtatg aaagatttga ttccccgtta taaaaccatg
     2941 tgtggttatt atgtacctcg caaagctggt tgggataccc atggacttcc tgtcgaatta
     3001 gaagtagaaa aaatgctggg attttccggg aaacaagata tagaaaatta tgggattgat
     3061 cgttttaacc agaaatgccg agagagtatc tggaaatata taaaagaatg ggaaaagatc
     3121 accgaacgca tgggaatttg gatggatatg gaccatccct atgtaacttg cgataatgat
     3181 tatattgaaa gtctttggtg gattctcaag caactcttta atcaaaatct tctctatcaa
     3241 ggatataagg ttttgcctta ttgttcacga tgtgggactt ctctttccag tcatgaagta
     3301 gcccagggtt atcaagatgt ggtagatccg tcaatttatc ttctttttca aatccagaat
     3361 cgagagaata cctttttttt agtttggaca accaccccct ggacattggt agcgaatgtt
     3421 gcattggcag ttggtcaaga cctcgattat gtggaaattg aagaaaaaga gactggcaaa
     3481 catctcatcc tcaatcggga aagtttgcca gcattatttc aacccgatgc atatactctg
     3541 attagagaaa tgaaggggaa agaactaatt ggcttgtcct ataatcctct aatgaccttc
     3601 ttggctgcgc caaaaggata tcaagtttat cacggagact ttgtttcaac tgaagaagga
     3661 actggaattg ttcacatagc tccagctttt ggagaagacg atatgaggtt ggctcaaacc
     3721 tttgatcttc ctgtgctcca tccggtgaaa gctgatggaa catatgatga gagcgtacca
     3781 ctctggcacg ggctttgggt taaagatgct gatcctttga tcataaaata tctccaatcc
     3841 accggtcagt tataccgaca agaaacccat cgccatacct atcccttttg ttggcgttgt
     3901 gatacaccac ttttgtatta tgctaaggga agctggttta ttcgttctac agcagttaaa
     3961 gaaactttat tagaaaataa tctcaaagtt cactggcatc cagatcacat ccaaagtggc
     4021 aggtttggaa atttcttgga gaacgtggtt gattgggctt taagccgaga acgatattgg
     4081 ggtactcctc ttaatatttg gaaatgtgaa agctgtggtt atcaagaagc tatagggtca
     4141 agacaagagc ttattcagcg tgccaatcag caaatcgatc aaattgagct ccatcggcca
     4201 tatgtcgatg ccataacctt aacctgttct cagtgtgggg gagagatgag aagaacgcca
     4261 gaggttcttg attgctggtt tgactcggga gcgatgtttg tagcccaaaa tcactatcct
     4321 tttgaaaat
//
LOCUS       DAOSOL010000101         4324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_20391_length_4324_cov_3.585149, whole genome shotgun sequence.
ACCESSION   DAOSOL010000101 DAOSOL010000000
VERSION     DAOSOL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20391_length_4324_cov_3.585149"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1338)
                     /gene="argH"
                     /locus_tag="PK171_03445"
     CDS             complement(<1..1338)
                     /gene="argH"
                     /locus_tag="PK171_03445"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT05290.1"
                     /translation="MKMRLWGSRMGKDLDQEVAEFSTSINEDQELYWFDIIGSLAHCK
                     MLQKIEILNQTEAQHIQRGLVQLLQNIEKNQINLNVYEDIHSAVEINLKQMIGEPAEK
                     LHTARSRNDQIVLDERLFLREKIIHLIRGINNLQKGLLTLAQKYPTLVMPGYTHLQPA
                     QPILFAHHLLAYFFMLQRDAERLKDALTRLNQLPLGAGALAGTSIPIDRKYVAELLAF
                     PSVQANSMDVVADRDYVLETLNALSIAFLHLSRFGEELVLWNSPGFQFIIIDDAFTTG
                     SSIMPQKKNPDIAELIRGKAGEVIGSWISLAVTLKGLPLTYNRDLQQDKPPLLRAVHE
                     CEKTFHIATRLINNIQPDSERMEAALQKGFLTATDLCEYLVGKGIPFRKTHEIVGSIV
                     RELVSQDKSFQDLSFQELENYIGRLDQEVALVLDEKQSINRKQSEGSTSPQEVK"
     gene            complement(1618..2826)
                     /locus_tag="PK171_03450"
     CDS             complement(1618..2826)
                     /locus_tag="PK171_03450"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT05291.1"
                     /translation="MSKRKKIVLAYSGGLDTSVAIRWLQEKYDADVFAVTLDLGQGKD
                     LEGVQKKALSIGAKEALVFDVREEFLSNYVLPALWAGAIYEKRYPLATALSRPLIAKY
                     LVQVAEEKGATYIAHGCTGKGNDQVRIEVSVAALNPDLQIIAPARIWGWTREEEIEWA
                     LANGIPIPVTVDSPYSIDVNIWGRSIECGVLEDPWTEPPEDIYQWTRSPEKAPDEAAT
                     IEIGFENGVPKLLKGQFYSLVNMVEELNTIGGDHGFGRIDHIENRLVGIKSREIYECP
                     AALLLLEAYKDLENLVMPREVTHFKSFLEEKYSLMVYEGLWFSPLREALDAFMQKIAE
                     RVSGSIRIKLYKGSMQITGRKSENSLYDFALATYDKGDRFDHASAEGFIKLWGLPTKI
                     DAIARKKTNQ"
     gene            complement(2907..4127)
                     /locus_tag="PK171_03455"
     CDS             complement(2907..4127)
                     /locus_tag="PK171_03455"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOT05292.1"
                     /translation="MNSEQVIQMENENMLATYARLPIVIKTGKGCMVYDPEGKSYLDF
                     VAGIAVNLLGYGYQPLIQAIFEQAQELIHASNLYYTLPQTQLAKDLIDISGFDRVFFN
                     NSGAEANEIALKMARFYGRRKHGNRYKFITFSRSFHGRTFLTLTATAQEKFHHDLEPL
                     VSGMSYAEFNQIESVMKVIDDETCGIIVEPIQGEGGIFCSKPSFLHQIRDICNERDII
                     LIFDEVQCGMGRTGRFFAFETYGVKPDIVTLAKGLGGGLPIGAVLVNEKIVNITRKGD
                     QGSTFGGNPICSRAAQIVVNTIAQTDFLKNVIETGEYLQQQLYQTQQKFNDLIQEVRG
                     IGLMWGLQVTGKAKLIVEKMLEKGVIVNAANDDVVRLLPPLIVGKKEIDQMVKSLVEV
                     LLDIQFTNTVPGMK"
BASE COUNT         1263 a          951 c          712 g         1398 t
ORIGIN      
        1 ttttacttcc tggggcgagg tactcccctc tgattgcttt cggtttattg attgcttttc
       61 atcaagaacc agtgcaactt cctgatccag tcgtccaata taattttcaa gttcttgaaa
      121 actcaagtct tgaaaacttt tatcttgaga aactaactca cgtactattg aacccactat
      181 ttcatgagtc ttccgaaaag ggatgccttt cccaactaaa tactcacaga ggtcagtggc
      241 ggtaagaaat cccttttgca gggcggcttc cattctttct gaatcaggtt ggatattatt
      301 aatcaagcga gttgctatgt gaaaggtttt ttcgcattcg tgaacggctc tcaaaagagg
      361 cggtttatct tgttgtaaat cacgattata ggttaaagga agtcctttca aggttaccgc
      421 taaactgatc caggatccaa taacttctcc ggctttcccc ctgatgagtt cggcaatatc
      481 cggatttttt ttctgtggca taatactcga accagtcgta aaagcatcat ctatgataat
      541 aaattgaaaa cctggtgagt tccaaagtac taattcctca ccaaaacggc ttaaatgaag
      601 aaaggcaatt gataacgcat tcagagtttc aagaacataa tcccgatcag ctactacatc
      661 catgctgttt gcttgtaccg aaggaaatgc cagcagttca gctacatatt ttcggtcaat
      721 agggatcgaa gtcccagcta acgctccggc acccaagggc aattgattga gacgggtaag
      781 agcatctttt aacctctcag cgtctctttg taacataaag aaataagcca aaagatggtg
      841 agcaaaaaga atcggttgcg caggttggag atgggtatat ccgggcatta ccaaagttgg
      901 atatttctga gctagagtta acaacccttt ttgaaggttg ttgatacccc gaatgagatg
      961 aataattttt tcccttaaaa acaagcgctc atcaagaact atttgatcgt tacgacttct
     1021 cgccgtgtgt aatttttcag ctggttctcc aatcatttgt ttcaaattta tttccactgc
     1081 gctatgaata tcctcatata cattgagatt gatttgattt ttttcaatat tttgtaacag
     1141 ttggacaagg cctctttgaa tatgttgagc ttcagtttgg tttaatattt ctattttttg
     1201 aagcatttta caatgagcaa gactaccaat aatatcaaac caataaagct cttggtcttc
     1261 attaattgaa gttgaaaatt cagcaacttc ttggtccaaa tccttcccca tccggcttcc
     1321 ccacaagcgc attttcatca ccaacttaga atattttgat actagtaata tttccaaaca
     1381 ctgtgaacaa cagaattcat aaaatttaaa gattacaata ttttactata atatataaaa
     1441 atagagggca caatcaactc gcacccccta tttttttaca ggttattgcc cgaggcttaa
     1501 cttatgaagc cccattattt atctagatag ttctgtgcta cgatctcacc atggttgaaa
     1561 agacatttcc ggttatataa tgcccttcca aaggaatgaa gtaactgatt cctgtcttta
     1621 ttggttggtt tttttccgag caatggcatc tatttttgtc ggcaaccccc agagcttaat
     1681 aaacccttct gccgatgcat gatcaaaacg atcaccttta tcataagttg ccaaagcaaa
     1741 atcgtataag gagttttcgg attttctccc agttatctgc atactacctt tatagagctt
     1801 tatccgaatt gatccactca ctctttcagc aattttttgc ataaaagcat ctaaagcttc
     1861 tcgaagcgga gaaaaccaaa gaccttcgta aaccattaaa gaatattttt cttctaaaaa
     1921 cgatttgaaa tgagttactt cccggggcat aactaagttt tctaaatctt tatatgcctc
     1981 gaggagaaga agcgcagctg gacattcata aatttcacgg gattttattc ccaccaagcg
     2041 gttttcgata tgatctattc ttccaaaacc atgatcgcca ccaatagtat ttaattcttc
     2101 gaccatattg accagtgaat agaactgacc ttttaataac ttgggtactc cgttttcaaa
     2161 accaatttca atggttgctg cttcatcagg tgctttttct ggtgaacgag tccattggta
     2221 tatatcttca gggggctcag tccaagggtc ttctaaaaca ccacattcaa tacttcgacc
     2281 ccagatatta acatcaatac tataaggact atcgaccgtt actggaattg gtatcccatt
     2341 ggcaagagcc cattcaattt cttcctcacg agtccaaccc catatccgag ctggagcgat
     2401 aatctgtaag tcaggattta aagctgctac cgatacttcg attctaacct ggtcatttcc
     2461 ctttcccgtg caaccatggg caatataagt tgctcctttt tcttcggcta cttgaaccag
     2521 gtattttgca atcaacggcc tcgataatgc tgttgctaag ggatagcgtt tttcataaat
     2581 agcacctgcc catagagctg gtaaaacata attactgaga aattcttccc ggacatcaaa
     2641 aaccaaggct tccttagctc caatgctcag agcctttttt tgaactccct ccaggtcttt
     2701 tccctgaccc aagtctaagg ttaccgcaaa aacatcggca tcatattttt cctgaagcca
     2761 gcgtatagca actgaggtgt caagaccacc tgaataggca agaactatct tttttcgttt
     2821 actcatatta ttcctcctca atcgaactcg ccatctttcc atccatatct aaccaggtgg
     2881 aatacatggc gaatagtctt ttttccctat ttcattccag gtactgtatt ggtaaattga
     2941 atatccaata aaacctcaac taagcttttt accatttgat caatttcttt tttaccaaca
     3001 ataagaggtg gaagaagcct gactacatca tcgtttgctg cattgactat aacccctttt
     3061 tccaacatct tttcgacgat taatttggct tttccggtaa cttgtaaacc ccacattaaa
     3121 ccaatgccac gaacttcttg gatcaaatca ttaaactttt gttgggtttg ataaagttgt
     3181 tgttgtaaat actccccggt ttcaataaca tttttcagaa aatcggtttg agcaatcgta
     3241 ttgaccacaa tctgggcagc ccgagaacaa ataggatttc ctccaaaggt acttccttgg
     3301 tctccctttc gagttatatt cactattttt tcgtttacca aaactgctcc aatcgggagc
     3361 cccccaccta aacctttggc taaagtaaca atatcaggtt ttactccata ggtttcaaaa
     3421 gcaaaaaatc taccggttcg acccattcca cattgtactt catcaaatat cagaataata
     3481 tctcgctcat tacatatgtc acgaatttga tgaagaaaag aaggtttgct acaaaagatt
     3541 cccccttcac cttggatcgg ctctacaatg attccacagg tttcatcatc aatgactttc
     3601 ataacacttt ctatctgatt gaattccgca taactcatac cacttaccag gggttctaag
     3661 tcatgatgaa acttttcctg tgcagttgct gttaaggtta aaaaagttcg tccatggaag
     3721 gatcgagaaa aggttataaa tttataacgg tttccatgct ttcttcttcc atagaaacga
     3781 gccattttta aagctatttc attggcttca gccccactat tattaaaaaa tactcgatca
     3841 aatccagaaa tatcaatcaa gtccttagct aattgagttt gaggaagagt gtaatagaga
     3901 ttggaagcat gaataagctc ctgagcttgc tcaaagatag cttgaattaa gggttgatac
     3961 ccatatccta aaagattcac agcaattcct gcaacaaaat ccaaatatga tttcccttct
     4021 ggatcatata ccatacatcc cttgccggtt tttattacga ttggtagtcg agcataggtt
     4081 gccaacatgt tttcgttttc catttgaata acttgctcgc tattcatatt tcaccctttt
     4141 ttaaaattat tttctatctg ctttgataaa ccgagtaatg ggtttattcc tttttttaac
     4201 catggaacca tccttttttc aattccataa ttctataaat tgctacttat cactgctcaa
     4261 ttcacattga ttatcatctt ttatcttgta atccgagttc ctgggacgaa ctttcctccc
     4321 aata
//
LOCUS       DAOSOL010000102         4296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_20550_length_4296_cov_4.229899, whole genome shotgun sequence.
ACCESSION   DAOSOL010000102 DAOSOL010000000
VERSION     DAOSOL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20550_length_4296_cov_4.229899"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PK171_03460"
     CDS             <1..474
                     /locus_tag="PK171_03460"
                     /inference="COORDINATES: protein motif:HMM:NF037386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05293.1"
                     /translation="RLAIPGLFLRTTMMVGFPGETDAIFNQMVSVIKSFRFERLGCFV
                     YSPQSNTPATQLSHQVEDEVKQARYQKIMKIQKKIAQDFHRSLIGKTLEVILDQGPLP
                     KKSSIFLGRTYGDAPEVDGQIKVKVNKNLNILQSGDILRVTINKSSAYDLEGIYP"
     gene            471..1727
                     /locus_tag="PK171_03465"
     CDS             471..1727
                     /locus_tag="PK171_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOT05294.1"
                     /translation="MKSAILCIGTEITTGLVKDINAHFLAFNLTAVGLSPKLVLFVPD
                     QKETITKTLQFIFEDEEIQSIFITGGLGPTQDDLTREILAEVIGKKLCFMPEQWDNII
                     RFYQKIRNTLPPENNKKQAFIPEGSLLLENPLGTAPGFVSDFKDKKIFVIPGVPKETE
                     YFWPIIKTHLPSNDNQFYRSEMIKICGVGESQFETELQPFLSSLPDTLQSAYLPVFGE
                     VWFYLYSYQKNHQVIQSAENIIKKIKEVWKSFLFSQWGNTLEQACGKLLREKRLTIAT
                     AESCSGGLLSHRLTNIPGSSAYFYRGYVVYSNQAKIDTVGVPSSLIAEYGAVSEEVAR
                     ALAEGVREKSHTDIGIGITGIAGPSGGSKQKPVGLVYIGISNFQHTQVKKHLFSGDRE
                     VIKMLSTQSALTQLFLQLQRGINDER"
     gene            1714..2289
                     /gene="thpR"
                     /locus_tag="PK171_03470"
     CDS             1714..2289
                     /gene="thpR"
                     /locus_tag="PK171_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOT05295.1"
                     /translation="MMNDNPIRCFVAFDFSPEAKAFIHSIIEKNQTRLPQARWVQPNQ
                     IHVTLQFFAALLPTQVNQVKLIIQNFFPLIESFPSTLAEIGAFSSWNRARVIWLGLDL
                     RSGEIMKQATQVFNEECTKQGIAVDRTRPFSPHLTLARLKNPVSILPQQLFQPTCYTT
                     SIRQVSFYQSTLTPRGPIYSPLITIPLKEVK"
     gene            2290..3300
                     /gene="recA"
                     /locus_tag="PK171_03475"
     CDS             2290..3300
                     /gene="recA"
                     /locus_tag="PK171_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT05296.1"
                     /translation="MTDKEDMVKQAINMIEKKYGKGSIMLLNQETMTTDIEIIKTGSV
                     LLNMALGVGGIPRGRVVEIYGPEASGKTTIALHIIAEAQKKGGIAAFIDAEHALDPIY
                     AGKIGVQVDKLLISQPSTAEEALDIVDTLVRSGAVDAVVLDSVAALVPRAELEGTIGE
                     QIIGLQARLMSQALRKLTGFISKTRTVAIFVNQLRETIGNIYGPTETTPGGRALKFYA
                     SVRIDVRKRDFIRITDNEIVGTKTRIKVVKNKVASPFKEAEIELIYGEGISKEGEVLT
                     IGENTGLVKRAGSWFSFNDNRLGQGRENTRSFLKENPDVASQILIAALEKMNVDPNLV
                     MN"
     gene            3305..3760
                     /locus_tag="PK171_03480"
     CDS             3305..3760
                     /locus_tag="PK171_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOT05297.1"
                     /translation="MKKKLTVEERIVRMICKSWYTKKRLREKLISEGYPENDVEEALQ
                     AFEQDGYIDDEFFMKTYLEGKRNSNPRGFWAYKTELERLGIEKDLLEQFRSIYFPPSE
                     EVKDGIELIRKWFQQGETSRDRIIQRLSRKGFSYDIAEWSWNLFQANRE"
     gene            3961..>4296
                     /locus_tag="PK171_03485"
     CDS             3961..>4296
                     /locus_tag="PK171_03485"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOT05298.1"
                     /translation="MLLVYILIGIVVGIGLGFKAGMNFKLKQLQQKKEDVEQKIQELL
                     QDANREAETIRKEALLSSKEQILREKQILEEEIKKKRKDLQGFEARLLRREEMLERRS
                     DQIEKRENHL"
BASE COUNT         1426 a          819 c          860 g         1191 t
ORIGIN      
        1 cgtttggcaa taccaggttt atttctacgc accaccatga tggttggatt tcccggagaa
       61 actgatgcta tcttcaatca aatggtgtca gtgataaaat cctttcgatt tgaacgatta
      121 ggatgttttg tttattcccc acaatctaat acacccgcta cgcagctatc tcatcaagtc
      181 gaagatgaag ttaaacaagc acgttatcaa aagattatga aaattcagaa aaaaatagct
      241 caggattttc atcgctcttt aatcgggaag actttggaag taattttgga tcaagggcct
      301 ctccctaaga aatcatcaat ctttttaggt cgtacctatg gtgatgctcc tgaagtcgat
      361 ggtcaaatca aagttaaagt caataagaat ttaaacatct tacagtcggg tgatatttta
      421 agggtaacta tcaataagag ttcagcctat gatttggagg gaatatatcc gtgaaatcag
      481 caattctttg cataggaacc gaaataacga ctgggctggt gaaagacata aatgcccatt
      541 ttttagcttt taaccttaca gcagttggcc tttcccctaa gttggttctg tttgtacccg
      601 accagaaaga aaccattacc aaaaccttac aatttatatt tgaagacgag gaaattcaat
      661 cgatatttat aaccggtggc ttgggtccaa cccaagatga cctgactcga gaaatattag
      721 ctgaagttat aggaaaaaag ttatgtttca tgcctgaaca atgggataat attattcgat
      781 tttatcaaaa gattcgcaac actctgccac ctgaaaataa caaaaagcag gcttttatcc
      841 ccgaaggatc ccttctctta gaaaatcctc tgggaaccgc ccctggattt gtttccgatt
      901 ttaaagataa aaaaatattt gtcatacccg gagtaccaaa agaaacagaa tatttttggc
      961 caatcataaa aacccatctt ccttcaaatg ataaccaatt ttatagaagt gaaatgataa
     1021 aaatatgtgg agtaggggag tctcaatttg aaaccgaact ccagcctttt ttatcttctc
     1081 ttccggatac cttgcaatca gcgtatcttc ccgtttttgg agaggtatgg ttttatctct
     1141 attcttatca aaaaaatcat caggttattc aaagtgctga aaatatcata aaaaaaatca
     1201 aggaagtatg gaaatccttt ctcttttctc agtgggggaa caccttagaa caggcctgtg
     1261 gaaagcttct cagggaaaag cgcttaacca ttgctacagc agaatcttgt agcggggggt
     1321 tattgagcca tcgcttaacc aacattcccg gaagttcagc atatttttat cgtggatatg
     1381 tagtctatag taaccaagcc aaaatcgaca cggttggagt gccttcatca ttaattgctg
     1441 agtatggtgc tgtatcagaa gaagtcgctc gagcccttgc tgaaggagtg agagaaaaat
     1501 cccataccga tatcgggatt ggaatcaccg gcattgctgg tccttccgga ggttcgaagc
     1561 aaaaacccgt cggattagtc tatattggaa tatccaattt tcaacatact caggttaaga
     1621 aacatctctt ttccggcgat cgggaggtta tcaaaatgct ctcgacgcaa agcgcgctta
     1681 cccaattatt tcttcaactc cagaggggga taaatgatga acgataatcc catccgctgt
     1741 tttgttgctt ttgatttttc tccagaagct aaagccttca ttcattcaat aattgaaaaa
     1801 aatcaaactc gtttacccca agctcgatgg gtgcaaccta atcagattca tgtcactctc
     1861 caattttttg ctgcattatt acccactcaa gtcaatcagg taaaattaat catccaaaac
     1921 ttctttccac tgattgaaag ctttccttcc accttagcag aaatcggcgc attttcttcc
     1981 tggaatcgag cacgggttat ttggcttgga cttgatcttc ggtcgggtga aataatgaaa
     2041 caagcaacac aggtttttaa cgaagaatgt acaaaacaag gtatcgcagt tgatcgaacc
     2101 cgtccttttt ctccccatct taccctggct cggcttaaaa atccggtttc tattcttcct
     2161 caacaattgt ttcaaccaac atgttatact acttctatca gacaggtatc attttatcaa
     2221 agcactttga cgcctcgggg accaatttat tcccctttga ttaccatacc gctgaaagag
     2281 gtgaaataat tgacagacaa agaagatatg gtaaaacaag ccatcaatat gattgaaaaa
     2341 aaatacggta agggctcaat tatgttattg aaccaagaaa ccatgacaac cgatattgaa
     2401 attattaaaa ccgggtcggt tctccttaac atggcattag gagtaggtgg tattcctcgc
     2461 ggacgagtgg tggaaatata tggcccggaa gcttcgggaa aaaccaccat tgcacttcac
     2521 ataattgccg aagcccaaaa aaaaggtgga atagctgctt ttatagatgc cgaacacgct
     2581 cttgatccca tctatgcagg aaaaatagga gttcaagttg acaaacttct aatctctcaa
     2641 ccgagtaccg ccgaagaagc tttggatatc gtcgatactt tggtccggag tggagcagtg
     2701 gatgcggtag ttttggattc ggtagccgcc ttagttccac gcgccgaatt ggaagggact
     2761 attggagaac aaattattgg actccaagca cggcttatgt cccaagcact acgaaagtta
     2821 accggattta taagtaaaac cagaaccgtc gctatctttg taaatcagtt gcgagagacc
     2881 attgggaata tttatgggcc aactgaaacc acgcccggag gacgagcgtt gaaattttat
     2941 gccagtgtac gcattgatgt ccgaaaaaga gactttatac gcattaccga taatgaaatt
     3001 gttggaacca aaactcgaat taaagtggtt aaaaataaag tggcctctcc ttttaaagaa
     3061 gcagaaatcg aattgatata cggggaggga atatcgaaag aaggggaagt tctcaccatt
     3121 ggagaaaata ccggattagt gaaacgagct ggaagttggt tttcttttaa tgataatcgt
     3181 ttaggacagg gacgagaaaa tacccgctct ttcttaaaag aaaacccgga tgttgccagc
     3241 caaattctga tcgcagcttt ggaaaaaatg aatgtagacc caaatttagt catgaattaa
     3301 cctaatgaaa aaaaaactaa ctgttgaaga acgaattgtt cgaatgatct gtaaatcctg
     3361 gtataccaaa aaaaggctca gagaaaagct aatatccgaa ggatacccag aaaacgatgt
     3421 cgaggaagct ctccaagctt ttgaacaaga tggatatatt gatgatgaat tttttatgaa
     3481 aacctacttg gaaggaaaaa gaaacagcaa tccacgtggt ttctgggcat ataaaactga
     3541 attggaacga ttgggtatcg aaaaagattt acttgaacaa tttcgatcta tttattttcc
     3601 cccttccgaa gaagtcaaag atggaatcga actcatccgg aaatggtttc aacaaggtga
     3661 aaccagtcgg gatcgaatta tccaacgtct atcacgaaag ggatttagtt acgatattgc
     3721 cgaatggtca tggaacctat tccaagccaa tcgggaataa gagttatctt gacttgatct
     3781 ttgcccatcg gtaaaatatt ctctatgcag ttttttaaca tagcttttaa tcaataccaa
     3841 aatttatttt attggtaaga aggaaaatcc gaaaattctt cgaacatcga atagaatcag
     3901 tcaataccca tcaggctttt tattcgtact ttgaaaattg aataaggggt gatcgcacaa
     3961 atgttgcttg tatatatatt aataggtatt gtggttggaa ttgggttagg attcaaagca
     4021 ggaatgaatt ttaaactaaa acaattacaa caaaaaaagg aagacgtcga gcaaaaaatc
     4081 caagaattgc ttcaggatgc gaatcgtgag gctgaaacta ttagaaaaga agctttactc
     4141 agctccaaag agcaaatcct tagagaaaaa caaatcttgg aagaagaaat caagaaaaaa
     4201 aggaaagact tacagggttt tgaagctcgt ttgttaagaa gagaggaaat gttagaaaga
     4261 cgaagcgacc agatcgaaaa aagagaaaat catctt
//
LOCUS       DAOSOL010000103         4261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_20789_length_4261_cov_3.828578, whole genome shotgun sequence.
ACCESSION   DAOSOL010000103 DAOSOL010000000
VERSION     DAOSOL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20789_length_4261_cov_3.828578"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PK171_03490"
     CDS             <1..761
                     /locus_tag="PK171_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOT05299.1"
                     /translation="YLQQNYSFEVFGKEGAQVMLLNNDPFGRWVNGSVGKVVQCNQAK
                     ENESSSVIVELTDGDLVKVEPYTWEIFEYQYDEGDSLIKTQTLGSFTQLPLRLAWAIT
                     IHKSQGKTFDRIVLDLERGAFSPGQVYVALSRCTSLTGIVLRQPIQKKHIFADWKVAD
                     FLTRFQYQLSDEKMPLEEKLRFLQEAIEAKKKLEIIYLKTNDEKSKRIIEPFYLGELD
                     YQGKKFFGLEAYCFKRKELRHFRVDRILEMKEVE"
     gene            1613..3145
                     /locus_tag="PK171_03495"
     CDS             1613..3145
                     /locus_tag="PK171_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT05300.1"
                     /translation="MLAKILSATAIGIDATIVEAEIDVGPGMPSFNIVGLPDTAVQEA
                     RERVRSALKNSGFTFPARRVTVNLAPASLRKTGSDFDLAIALGILAATGQTHFNSDRT
                     IVVGELSLSGEIRPVQGILPIAHTLSKEASIYRLFVAGGNAREGAIAGKVEVYGFRDI
                     NQLSDFLNGNQSMIPTTIDPQSLFIYSNHEEDLSEVKGQRHGKRALEISAAGGHHLLF
                     VGPPGSGKTMLSKRLPGLLPLLSLEEAIEVTKIHSVAGLISADQPLVISRPFRSPHHT
                     ISDVALIGGGQLPRPGEISLSHHGVLFLDEVLEFKRNVLEALREPLEEGKITISRASM
                     TTTYPAKFTLVASCNPCPCGFLGDTKKTCTCSTSQIIRYRSRLSGPFLDRMDLQVEIP
                     RLDFSDLSSNTVEENSETVRKRVEQTRLLQQERYQKEGIHLNGEMKVRYIKKFCVCSR
                     EAHDLLKKSIETLALSARAYHRILKVARTIADLDRSEKIDVEHVAEAIQYRTLDRKYL
                     EF"
     gene            3148..4236
                     /gene="dprA"
                     /locus_tag="PK171_03500"
     CDS             3148..4236
                     /gene="dprA"
                     /locus_tag="PK171_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT05301.1"
                     /translation="MKDLPYWVAFNHLPGIGPVRFQKIIETFGSASDAWRASPKDLAH
                     CLGKKIVEEILPVKLSLQPEAVFQKIIKRGIQILPISDERYPALLRDITYPPYLLYQR
                     GDFDFRSYKKWIALVGTRRATTYGKKVARYLARSLAEEGWVVVSGLALGIDGEAHYGA
                     LEGEGKTVAVLGTGVDIVYPGSNRGLAERILHQKGALISEFVPGFGPQKESFPVRNRL
                     ISGLSLGVVVVEAPLKSGALITVNFALEQGREVMAVPGSIFSFQSEGTHQLINQGARL
                     VHNVADIVEELGFLASPQSKMKKKIALPDLDEDEKELLNLLDISGCYLDELLAKSGWE
                     SGRFYKVLLSLEMKKVIQTFAGGKIGLG"
BASE COUNT         1248 a          817 c          954 g         1242 t
ORIGIN      
        1 cctacctcca gcaaaattat agctttgagg tctttggaaa agaaggagct caggtgatgc
       61 ttttaaataa cgatcccttt ggtcgttggg tgaatggaag tgttgggaag gttgtccaat
      121 gtaatcaagc caaagaaaat gagtcatcct cagtgattgt tgaattaacc gatggagatc
      181 tagttaaagt agaaccttac acctgggaga tcttcgaata ccagtatgat gaaggagatt
      241 cattgattaa aacccaaaca ctgggttctt tcacccagct tcctttgcgg ctggcttggg
      301 cgattaccat ccataaaagc caggggaaga cttttgatcg gattgttctg gatcttgaga
      361 gaggggcttt ttcacccggg caggtttatg tggctttgag ccggtgcacc tcacttacag
      421 gtattgtgct cagacaacct attcaaaaaa aacacatctt tgccgactgg aaagttgcag
      481 atttcctcac ccgttttcaa taccagctat ctgatgaaaa gatgccatta gaagaaaagc
      541 tgcgttttct tcaagaagct attgaagcca aaaaaaaatt ggaaattatt tatctcaaaa
      601 ccaacgatga aaaatcaaaa cggataatcg aaccatttta tttgggcgaa ctcgattacc
      661 aggggaaaaa gtttttcggg ctcgaagcct attgttttaa aagaaaagag ctgagacatt
      721 tccgagtcga tcgaattctt gagatgaaag aagtcgaata atactcattt caattttcat
      781 cctcggagag gtgccgcttt gcggcgggga gggtgtccct tttttaaaag ttatctttag
      841 aaattgatta agaaatatac ataaaagatg agattctcac ttcgaacaat acgctcctca
      901 gaattttact tcttttcaac atctgctttc tcaagcttat caaccatttt cgaagaagcc
      961 ttaactattt cctggcttaa ctggtttata tctttattga ttttgcgaat gtgacggttg
     1021 ataaggacaa ggaaaaataa aggaagggat atgagtgtga tgtataaaaa aaggatatac
     1081 aagttatgat tattttctat tattaaagaa aatgaggcca aaattagtgc gttaggtaaa
     1141 cctacaaagg ttgaagggat tataaacccc ctcctattaa agacttttat attattgaat
     1201 aatcatgaaa attattcata ggtaattaat atcatattca aagaaaaaat tctacattct
     1261 aaatgaggtc aatggtgata tttttggtct tcaaacccat tgattattac ttttaattga
     1321 ccacactaat aaacaaatat ttatgattat ttttccaaag ttaaaatttt tttaagataa
     1381 aaagatctta ttggttgctt cctaaattaa aaaaataaaa actgtaacga gggaaaaaga
     1441 aggagttatc aggaatattc agagaccggg aagagaaatg gctttttaat aatagtcatt
     1501 atctattatt ttttatataa taaatattat gagataataa aaatcattga attaaatata
     1561 ataatgatta taaagatgtt gttcggctct aaccagagaa ggaggattgg aattgttagc
     1621 gaaaatttta agtgctactg ccattggtat tgatgcgact atagttgaag ccgaaatcga
     1681 cgttggtccg ggcatgcctt cctttaacat tgttggtctt cctgatacgg cagtgcagga
     1741 ggctcgagaa cgagtccgta gtgctttgaa aaacagtggt tttacttttc ctgcccgccg
     1801 ggttactgtt aatctggcac cggcatctct cagaaaaact ggcagtgatt ttgatttagc
     1861 catcgctctg ggaatcttag ctgctaccgg tcagacacat tttaattctg accgaaccat
     1921 tgtggtagga gagctatcgc tttctggtga aatacgaccg gtacagggga ttctaccgat
     1981 tgctcacacc ctttctaaag aagcgagcat ctatcgtttg tttgtagccg gtggaaatgc
     2041 tcgagaaggt gctattgccg gaaaagttga ggtctatgga tttcgtgaca tcaaccagtt
     2101 atcagatttt ctcaatggga atcaatccat gattcccact accatcgatc ctcagagttt
     2161 attcatctat agcaatcatg aagaagattt gtcagaagtc aaaggacagc gtcatggaaa
     2221 gcgagctttg gaaatttcag ctgccggtgg tcatcacctt ctttttgttg gtccacccgg
     2281 atcgggaaag accatgttat caaaaaggtt acctggatta ctgcccttat tatcactgga
     2341 agaggcaatt gaggtgacta agattcatag cgttgccgga ctgatatccg ctgaccaacc
     2401 cttagttatc agccgtcctt tccggtctcc ccatcatacc atttccgatg tggctctgat
     2461 tggtggtggt caattgccgc gacctggaga aattagtctt tcccatcatg gagtactttt
     2521 tcttgatgaa gtgttggaat ttaaaagaaa tgtattggaa gcgttacgcg aacccctcga
     2581 ggagggaaag attaccatct cccgagccag tatgaccaca acttacccag caaaatttac
     2641 tctggtcgca tcatgtaatc catgcccctg tggcttcttg ggggatacca agaaaacctg
     2701 cacttgttcg acctcccaaa tcatccgcta tcggagtcga ttatccggtc cttttttgga
     2761 tcggatggac ctccaagtcg aaatccctcg gttggatttt tccgacctgt cctccaatac
     2821 cgttgaagaa aactcggaaa cggtgagaaa aagagttgaa caaacccggc ttctccagca
     2881 ggaaagatac caaaaagagg gaattcatct gaacggggag atgaaagtcc ggtatattaa
     2941 aaagttctgc gtctgtagtc gagaagccca cgatcttctt aaaaagtcca ttgaaacctt
     3001 ggcattgagt gctcgagctt accatcggat tttaaaagtt gcccgtacca tagccgacct
     3061 tgaccgctcg gaaaaaattg atgttgagca cgttgctgaa gctattcagt atcggacatt
     3121 ggatcgaaag tatctggagt tttaaaaatg aaagatcttc cttattgggt ggcatttaat
     3181 catttaccag gcattggtcc ggttcgtttt cagaaaataa ttgagacctt tgggtcagca
     3241 agtgatgctt ggagggcttc tccaaaagat ttagctcatt gtctggggaa aaaaatcgtt
     3301 gaagaaattc ttccggttaa attgtcactt caaccggaag ccgttttcca aaaaatcatc
     3361 aaacggggaa tccaaatcct ccccatttcc gatgaacgat atccggcttt attgagagac
     3421 ataacgtatc caccttatct gctttaccaa cgaggagatt ttgatttccg ttcctataaa
     3481 aaatggattg ccctggttgg aacccgacga gccaccacct atggaaaaaa agttgccagg
     3541 tatttggctc gctcgctggc tgaagagggt tgggtcgtgg tgagtggttt agctctggga
     3601 attgatggag aagctcatta tggtgccttg gaaggagagg gaaaaaccgt tgctgttctg
     3661 ggaaccggtg tcgacattgt ctatccaggg tcaaatcgag gattggccga gcggattctt
     3721 caccaaaagg gtgccttgat cagtgagttt gttcctggtt ttgggccaca aaaggaaagt
     3781 tttccggttc ggaaccgact cattagcggc ctttctttgg gtgtggtggt cgtagaagct
     3841 cctttaaaaa gtggggcctt gattaccgtt aattttgcct tggaacaggg gagagaagtg
     3901 atggccgtgc ccgggtcaat cttttccttc cagagcgaag gcactcacca gctcatcaac
     3961 caaggagcgc gattagtcca caatgttgct gatattgttg aggaattggg atttctggca
     4021 tcgccccaat cgaaaatgaa gaaaaaaata gccctccccg atttggacga agatgaaaag
     4081 gaactcctca atctacttga tatttctggg tgttatctgg atgaactact tgccaaaagc
     4141 ggctgggagt ctggtcgatt ttacaaggtc ctcttatccc tggaaatgaa aaaagtcatt
     4201 caaacctttg ctggcgggaa aataggctta ggttgattga agctcagggg ttttttattt
     4261 t
//
LOCUS       DAOSOL010000104         4245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_20891_length_4245_cov_4.247255, whole genome shotgun sequence.
ACCESSION   DAOSOL010000104 DAOSOL010000000
VERSION     DAOSOL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20891_length_4245_cov_4.247255"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PK171_03505"
     CDS             <1..504
                     /locus_tag="PK171_03505"
                     /inference="COORDINATES: protein motif:HMM:NF005309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-threitol dehydrogenase"
                     /protein_id="HOT05302.1"
                     /translation="RIDILVNNAGVGMLDDAENLSEEIWDKTMAVNLKAPFMLSQIIG
                     REMIKQKKGKIINIASQAGLIALDKHVAYCSSKAGIIGMTKVLALEWAEYGINVNAIA
                     PTVILTELGIKAWSGEVGEAMKKKIPLRRFGYPEEVAAAALYLASEASNLVTGETLVI
                     DGGYCIQ"
     gene            621..1913
                     /locus_tag="PK171_03510"
     CDS             621..1913
                     /locus_tag="PK171_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05303.1"
                     /translation="MKLKSLILLGVMILVLGMTSIAFGWSLEEAAKPYAGVTLRFITE
                     TTPPSYWVEEVVPEFEKATGIKVIVERQAHPHLEEKALMDFASKTGIYDVFNFDYSWT
                     GKYVKAGYIEPFEQYMDNPALVNPDNALDDFYPRMWEGTMWDGKAYGYPFDSVIQYLF
                     WNKAVYDKYGVAGPPKKPDEWMDTMQKLNHPPELYGTGMMAKRHLSVVCEWLCILWAF
                     QGEFYDDNYNVKFNDENGVKATEFYKKMAEFAPPGVTTWTWDDVSTALQQGTVAQSVQ
                     WDENYGSMENPEESRVVGQMRYAPVPSYGDPVSHYGGSSLGIPTSSKNKEAAFLFIQW
                     ATSQDIQLRGVGHGSSPTRMSVYGVQELRDKFPSFAATDEAAASTGWRPRIPEWDDMV
                     ETLALEINSVIAGAKDVKSALDTSAAKVEELLKTGGYK"
     gene            1984..2916
                     /locus_tag="PK171_03515"
     CDS             1984..2916
                     /locus_tag="PK171_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05304.1"
                     /translation="MSLKKLSVFQRFGFWISSTETKFKWALLLPTIILLAIITVYPTI
                     YAFNLSLSDYNLARAADTLKGYIGLDNFRNILKDPTFWSSLRTTITFTVVVVVVEFFL
                     GLCIALILQGTVTGSRIVRTFILLPMIMAPIVVGLTWTYLYQSEFGLVTFILRKLHLM
                     SYATAPLADITSALPALMVVDIWQWTPFLIMVLLAGLEALPTAPYEAARIDKASPFLV
                     FRKITLPLLQPVILIVLVLRIMDAIRVFDIVYALTEGGPGEATDVLSMFIYRNAFYLW
                     GIGKASAISFILLYIIMIVSVILVNLLSRAKQTV"
     gene            2969..3793
                     /locus_tag="PK171_03520"
     CDS             2969..3793
                     /locus_tag="PK171_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05305.1"
                     /translation="MEKNKTKTIIAIIIIGIILVVNVMPFLWLILTSLKTRLDIFAIP
                     PKFFFHPTAKNFISAFTKRHFLPMFYNSLIISASTTLLSLAVGTLGAYSLARFKLFGD
                     KHISFWILSTRMFPSIVLVIPFFIMATRWGLHDTRSLLVIVYTTFNLPFVVWIMRSFF
                     EDIPSDLEKAAMVDGYSRWDAFWRVILPLSAPGLVTTAILCFIFSWNEFLFANVLTAA
                     MAKTVPVGIRGLVTSRAIEWGEIAAVATLQVIPVLVFTFAVQKYIIRGLTLGAVKG"
     gene            3880..4080
                     /locus_tag="PK171_03525"
     CDS             3880..4080
                     /locus_tag="PK171_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05306.1"
                     /translation="MTTFGLGFLWLGWLEKYLPIWIVPLIGLGFALLLIIPWYRKFNR
                     ERIREKEELLQMRNGIVKRMES"
BASE COUNT         1180 a          737 c          960 g         1368 t
ORIGIN      
        1 cgaattgata ttttagtaaa caacgccgga gtaggaatgt tggatgatgc tgaaaatctt
       61 tccgaggaaa tctgggataa aacgatggca gtaaatttga aagctccttt tatgctctct
      121 caaatcattg gtcgagagat gatcaaacag aaaaaaggaa aaattatcaa cattgcttct
      181 caggcgggat tgatcgccct tgataagcat gtagcttatt gttccagtaa agccgggatt
      241 attggtatga cgaaagtatt agctttggaa tgggctgaat atggtattaa tgtcaacgct
      301 attgctccaa ctgttattct cactgaattg ggcataaaag cctggtcagg ggaagtggga
      361 gaagctatga aaaagaaaat tccccttcgt cgttttggtt atccagaaga agttgcagct
      421 gcggctcttt acctggctag tgaggcttcc aatttggtaa ctggtgaaac cctggttatt
      481 gatggtggat attgcattca ataatcggtt aaattatagg aatgagggag gtgagatttg
      541 gtaaaaaaat tttgtggtgt aatttttgta ttcgaacaga ttaattattt aacgaaatac
      601 cttaagaagg agggattgaa ttgaaactga aatctcttat tttattggga gtaatgatat
      661 tagttctggg aatgacgtct attgcctttg gatggagttt agaagaagca gccaaaccct
      721 atgccggggt taccttacgg tttataaccg aaaccactcc ccctagctat tgggtcgaag
      781 aagttgtacc tgaatttgag aaggcaaccg gtattaaagt tattgttgag cgtcaggccc
      841 atccacacct tgaagaaaaa gcattaatgg attttgcatc aaaaaccgga atatatgatg
      901 tcttcaactt tgactattcc tggacaggaa aatatgttaa ggctgggtat attgaaccct
      961 tcgagcagta tatggacaac cctgctttag tcaatccgga taatgctcta gatgatttct
     1021 atccaaggat gtgggaaggg actatgtggg atggcaaagc atatggttat cctttcgata
     1081 gtgtcatcca gtatttattt tggaataaag ccgtttacga taaatacggt gtagctggac
     1141 ctccaaagaa gccggatgaa tggatggata ccatgcaaaa gctcaatcat cctcccgaac
     1201 tctatgggac tggtatgatg gctaaacgtc acctctcagt ggtttgtgaa tggctctgta
     1261 ttttatgggc atttcaaggt gaattctatg atgataacta caatgtaaaa tttaatgatg
     1321 agaatggtgt gaaagctact gaattttata aaaagatggc agaattcgct cctcctggag
     1381 taaccacttg gacttgggac gatgtcagta ccgctcttca acagggtaca gtggcacaat
     1441 cggttcagtg ggatgaaaac tatggaagta tggaaaatcc agaagaatcg agagtggttg
     1501 gacagatgag atatgccccg gtaccatcct atggggaccc agtctctcat tatggtggtt
     1561 ccagcttagg tattccgact tcttccaaga acaaagaagc ggcattcctc ttcattcaat
     1621 gggcgactag tcaagatatc caactccgtg gagtaggtca cgggtcttca ccaaccagga
     1681 tgtcagttta tggagttcag gaactacgtg ataaattccc aagttttgcc gcaacagacg
     1741 aagctgctgc cagtactgga tggcgaccaa gaataccgga atgggatgac atggttgaaa
     1801 ctcttgcttt ggaaattaac tcagttattg ctggagcgaa agacgtgaag tcagctttag
     1861 atacttctgc ggcaaaagtt gaagaactct tgaaaacagg tggttataaa taaggcgtaa
     1921 tttttaagaa aattttgctg cacagagata tcaatctctg tgcagcaaaa gggggaaagt
     1981 cgattgtcac ttaaaaaact ttcggttttt caacgttttg gtttttggat atcgagcact
     2041 gaaaccaagt ttaaatgggc tttattgctc cccactatca ttttattggc tataataacc
     2101 gtttatccaa ctatttatgc ctttaacctt tcactctcgg attataactt agcacgagca
     2161 gcggataccc tgaaagggta tattggtttg gataatttcc gaaatatatt aaaagatccg
     2221 actttttgga gttctcttcg aacaacaatt acctttacag tggttgttgt tgtggttgag
     2281 ttctttttag gattgtgtat tgctcttatc ttacaaggaa cagttactgg ttcgagaatt
     2341 gttagaactt tcatcttgct accaatgatt atggcaccga ttgtcgttgg tttgacctgg
     2401 acttacctct atcagtcgga gttcggctta gttaccttta tccttcgtaa gcttcacctg
     2461 atgagttacg ctactgctcc gctggctgat attacgagtg cacttccggc attgatggtt
     2521 gttgatatat ggcaatggac accttttctt atcatggtat tattagctgg gttggaagca
     2581 cttcctactg caccctatga agctgcccga atcgataaag catcaccttt tctagtgttt
     2641 cggaaaatta ctcttccttt gcttcaaccg gttatcctca tcgttttagt tttaagaatt
     2701 atggatgcta tcagggtttt cgatattgta tatgcattaa ccgagggtgg acctggagag
     2761 gcgaccgatg ttttaagtat gttcatttat cggaatgcct tttacctgtg ggggattggt
     2821 aaagcttcag cgatttcgtt tatcttgctg tatattataa tgattgtttc ggttatttta
     2881 gtaaatcttt taagccgggc gaagcaaacg gtttgacccc atgagaaact gaatgggata
     2941 gacaatactg attttggggt ggttactctt ggaaaaaaac aaaacgaaaa ccataatcgc
     3001 tataattatt attgggatta ttttggtagt gaatgtgatg ccttttttgt ggctcatatt
     3061 aacctcctta aaaacccgac tcgatatttt tgctattcca ccaaaattct tttttcaccc
     3121 tactgcaaaa aattttatat cggcttttac caagcgtcat tttttaccga tgttttacaa
     3181 tagtcttatc atatctgcaa gtacgacttt actttcttta gcagttggaa ctcttggggc
     3241 ttattcgctg gctcgtttta aactttttgg agataagcac atttcctttt ggatcttaag
     3301 tactcgtatg tttccttcta tcgtattagt tattcctttt tttatcatgg caactcgttg
     3361 ggggcttcac gatactcgat cacttttagt gattgtttat accactttta atttaccttt
     3421 tgtggtttgg atcatgagaa gtttttttga ggacatacct agtgaccttg aaaaagcagc
     3481 catggtggat ggatactcca ggtgggatgc tttttggagg gtcattttac ctctcagtgc
     3541 tccgggttta gtaactactg caatactctg tttcatcttt tcttggaatg aatttttatt
     3601 tgccaatgtt ttaactgccg ctatggcaaa aactgtccca gtaggaatcc gagggttggt
     3661 tacttcgcgg gcgattgaat ggggtgaaat agctgcagtt gcaaccttac aggttattcc
     3721 cgttttagta tttacattcg cagttcaaaa atacatcatc cgcggtttaa ctttaggtgc
     3781 tgtgaaggga taataatctt taacgagtga ttcaaattgc tggaaagggc gtgaagtcca
     3841 gtggattttg gctttgggga tcgaattttt gtatcagtta tgaccacctt tggtcttgga
     3901 tttttatggc tgggttggct ggaaaaatat cttccaatct ggattgttcc tttgatcggt
     3961 ttgggtttcg ctcttctgtt aattattccc tggtatcgaa aatttaatcg agaaagaatc
     4021 cgggagaaag aagaattatt acagatgaga aacggcattg tgaaaaggat ggaatcttaa
     4081 atggcaagct taaccctgaa gaatgtatgg aaaaaatacg ggaaagtaat tgcattaaaa
     4141 ggagtaaatt gtgaagttaa ggacggagag ttattaacga ttttaggacc atcgggtgct
     4201 ggaaaaactt cactccttca aacgattgca ggagttgaag aaatc
//
LOCUS       DAOSOL010000105         4221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_21064_length_4221_cov_4.591935, whole genome shotgun sequence.
ACCESSION   DAOSOL010000105 DAOSOL010000000
VERSION     DAOSOL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21064_length_4221_cov_4.591935"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PK171_03530"
     CDS             complement(<1..443)
                     /locus_tag="PK171_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05307.1"
                     /translation="MNKKILFFTLLIFCIILYGCNKQHNLNENPIEEEPFEENPVEVT
                     MDEVKTWLIEQYNHYRVTSNIVFPTTDDSGQAKIETISFERKYLTNQGEFTAPMFDLE
                     AEVMFIIDMEEETDDVIVTLILQGYGDEFDETMVWLEDIIPQKIL"
     gene            complement(514..1401)
                     /locus_tag="PK171_03535"
     CDS             complement(514..1401)
                     /locus_tag="PK171_03535"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT05308.1"
                     /translation="MKKWQEIEQFLKLIHQDHIENQKYYMFGYQEEYKKMEVVNLGYI
                     PFYDGKETIRDIDILPDLYKKNRPSILKKRTKYICIHDTASGAPTADGKAHKRWLMNM
                     ANDKENQNSVSWHFTVDEGGIFQHLPTDEVAHHAGDGYREWGNTYFNTTYNRTCITGG
                     NLNSVGIETCVNFGSDYTRTMRNTAKLVAELMIEFDLTIDAIKQHNDFSGKDCPMTMR
                     HANRWQEFLHLVKIEHLGKTRFNDVVFNWKSLTPEIIDNTGKIINHINNGQVDYEVEV
                     EYNNEKRKYQFTGYLNHLS"
     gene            complement(1415..2515)
                     /locus_tag="PK171_03540"
     CDS             complement(1415..2515)
                     /locus_tag="PK171_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT05309.1"
                     /translation="MAKVYYIDFHTKSGKNMLQKLKNLIEEAGIEKINFEKRHVAIKI
                     HFGEPGNLAYIRPNYAKVIVDKVRSLGGKPFLTDCNTLYVGRRNEGLNHLETAYENGF
                     SPFSTNAHVIIGDGIKGTDDIEIPINLEYVKNAKIGRAIADSDIIISMNHFKGHESTG
                     FGGALKNLGMGCGSRAGKMEMHSSGKPRVNEVMCRFCKKCAEVCAHQAITYLATNKAK
                     INQDLCVGCGRCIGACAFNAIKPIWNESEEILVKKIAEYSLAVVKGKPNFHINFIMDV
                     SPDCDCASYNDVAIVPNIGILASFDPVALDQASVDMVNAAPAVEESSLKQKKHHQDKF
                     KMLHPDTKWEEGLIHGEKIGLGERKYELIKVK"
     gene            complement(2604..2679)
                     /locus_tag="PK171_03545"
     tRNA            complement(2604..2679)
                     /locus_tag="PK171_03545"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2644..2646),aa:Phe,seq:gaa)
     gene            complement(2686..2761)
                     /locus_tag="PK171_03550"
     tRNA            complement(2686..2761)
                     /locus_tag="PK171_03550"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2726..2728),aa:Asp,seq:gtc)
     gene            complement(2764..2839)
                     /locus_tag="PK171_03555"
     tRNA            complement(2764..2839)
                     /locus_tag="PK171_03555"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2804..2806),aa:Met,seq:cat)
     gene            complement(2850..2940)
                     /locus_tag="PK171_03560"
     tRNA            complement(2850..2940)
                     /locus_tag="PK171_03560"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2902..2904),aa:Ser,seq:tga)
     gene            complement(2969..3045)
                     /locus_tag="PK171_03565"
     tRNA            complement(2969..3045)
                     /locus_tag="PK171_03565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3009..3011),aa:Ile,seq:cat)
     gene            complement(3050..3126)
                     /locus_tag="PK171_03570"
     tRNA            complement(3050..3126)
                     /locus_tag="PK171_03570"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3090..3092),aa:Met,seq:cat)
     gene            complement(3159..3234)
                     /locus_tag="PK171_03575"
     tRNA            complement(3159..3234)
                     /locus_tag="PK171_03575"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3199..3201),aa:Ala,seq:tgc)
     gene            complement(3268..3354)
                     /locus_tag="PK171_03580"
     tRNA            complement(3268..3354)
                     /locus_tag="PK171_03580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3318..3320),aa:Leu,seq:taa)
     gene            complement(3360..3435)
                     /locus_tag="PK171_03585"
     tRNA            complement(3360..3435)
                     /locus_tag="PK171_03585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3400..3402),aa:Lys,seq:ttt)
     gene            complement(3439..3514)
                     /locus_tag="PK171_03590"
     tRNA            complement(3439..3514)
                     /locus_tag="PK171_03590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3479..3481),aa:Thr,seq:tgt)
     gene            complement(3521..3596)
                     /locus_tag="PK171_03595"
     tRNA            complement(3521..3596)
                     /locus_tag="PK171_03595"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3561..3563),aa:Val,seq:tac)
     gene            complement(3648..3762)
                     /gene="rrf"
                     /locus_tag="PK171_03600"
     rRNA            complement(3648..3762)
                     /gene="rrf"
                     /locus_tag="PK171_03600"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3800..>4221)
                     /locus_tag="PK171_03605"
     rRNA            complement(3800..>4221)
                     /locus_tag="PK171_03605"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1176 a          961 c          700 g         1384 t
ORIGIN      
        1 tctaatattt tttggggaat tatgtcttcc aaccatacca tcgtttcatc aaactcatca
       61 ccataacctt gaagaataag cgtaacaata acatcatctg tttcttcttc catatcgatg
      121 ataaacataa cttccgcttc caaatcaaac atcggtgccg taaactcccc ttgatttgtt
      181 aagtattttc gttcaaaaga tatagtttca atctttgcct gtccactatc atctgtagtg
      241 ggaaaaacta tatttgatgt tactcgataa tgattatatt gttcaattaa ccacgttttt
      301 acttcatcca ttgtcacttc tactggattt tcttcaaaag gttcttcttc aataggattt
      361 tcattgagat tatgttgttt attgcacccg tataaaatga tgcaaaagat taataaagta
      421 aaaaataaaa ttttcttgtt catagtattt cctcacaaca attttatcat atctacttta
      481 tttttaaaac aaaaaaagga agattttctc ttcctatgat aaatggttta aatatcctgt
      541 aaactgatat tttctttttt cattgttata ttcaacctct acttcataat caacttgtcc
      601 attgtttata tgattaatga ttttccctgt attatcgata atctctggtg ttaaactttt
      661 ccaattgaat actacatcat taaatcttgt ttttccaagg tgttcaattt ttaccaaatg
      721 taagaattct tgccaacgat ttgcatgtct catggtcatc ggacaatctt tccctgaaaa
      781 gtcattgtgt tgtttaatag catcaatagt caagtcaaat tctatcatca attccgcaac
      841 tagttttgcc gtgtttctca ttgtcctagt ataatcagaa ccaaaattaa cacaagtttc
      901 tatccccaca ctatttaaat tacctccggt aatacaagta cgattatatg ttgtattgaa
      961 ataagtgttt ccccattcac ggtaaccatc tccggcgtga tgcgccactt catcagttgg
     1021 taaatgttga aagatgcctc cttcatcaac tgtaaaatgc caagaaaccg agttttgatt
     1081 ttctttatca ttcgccatgt tcattaacca acgtttatgt gctttaccat cagctgtcgg
     1141 agccccgctt gcagtatcat gaatgcaaat atattttgtg cgttttttta aaatcgaagg
     1201 acgattcttt ttatataaat ccggcaaaat atcaatatct cttatcgttt cttttccatc
     1261 ataaaaagga atatatccta agttgactac ttccattttc ttatattctt cttgataacc
     1321 aaacatatag tatttttggt tttcaatatg atcttgatga attaatttta aaaattgttc
     1381 tatttcttgc cattttttca tttttcttcc ctttctattt cactttaatt aattcgtatt
     1441 ttctttcccc aaggcctatt ttttctccgt ggattaagcc ttcttcccat ttcgtatcag
     1501 gatgcagcat tttaaatttg tcttgatggt gttttttttg cttcaatgaa ctttcttcta
     1561 ctgctggagc agcatttacc atatcaacac tagcttgatc tagcgctact ggatcaaatg
     1621 atgcgagaat tccaatgttt ggaacaatcg ccacatcgtt gtagcttgca caatcacagt
     1681 ctggagatac atccataata aaattaatat ggaagttggg ttttcctttc acaactgcca
     1741 gtgaatattc tgctattttc ttgaccaata tttcttctga ttcgttccat attggtttga
     1801 tagcgttaaa agcacatgct ccaatgcaac gtccacaacc aacacataaa tcttggttga
     1861 ttttagcttt attggtcgct aaataggtaa ttgcttgatg ggcacacact tcagcacact
     1921 tcttacaaaa acggcacata acttcattaa ctcgtggttt tccactacta tgcatttcca
     1981 tttttccagc tcgtgaacca caacccattc ctagattttt caatgctcca ccaaaacccg
     2041 tgctttcgtg ccccttaaaa tgattcatag aaataatgat atcactatct gcaatcgccc
     2101 tacctatttt cgcatttttc acatattcta aattgattgg aatttcaata tcgtcagttc
     2161 ctttaattcc atctccgata attacatgag cgtttgttga gaagggagaa aatccatttt
     2221 cataggccgt ttctaaatga tttaatcctt cattacgacg tcctacatat aaagtattac
     2281 aatcagttaa aaatggtttc ccgcctaaag acctgacttt atcaacaatt accttcgcat
     2341 aattagggcg aatataagca aggttccctg gctcaccgaa atgaattttt attgcaacat
     2401 gtcgtttttc aaaatttatt ttttctattc ctgcttcttc gattaaattt ttaagcttct
     2461 gcaacatgtt tttccctgat ttggtatgaa aatcaatata atataccttt gccatcgctt
     2521 tcctccattt ttctatttac ataatatcac aaaaagtcct tttaagaaag aaaaaatcac
     2581 ccgtatggat gattatttct aaatggtggc tagaggcgga atcgaaccgc cgacacaagg
     2641 attttcagtc ctttgctcta ccgactgagc taccgagcct aaaaatggcg gtctcaacgg
     2701 gacttgaacc cgcgatcttc agtgtgacag actgacatgt taaccactac accatgagac
     2761 caatggttgc ggaggaagga tttgaaccaa cgaccttcgg gttatgagcc cgatgagcta
     2821 ccagctgctc tactccgcga tatattaaat ggcggaggaa gcaggattcg aacccgcgcg
     2881 cgctttcacg cctgtcggtt ttcaagaccg atcccttcag ccggacttgg gtattcctcc
     2941 actatttaca agatattaag ttttaaactg gtggacccgg taggactcga acctacgacc
     3001 gaccggttat gagccggtag ctctaaccaa ctgagctacg ggtccacaat ggtagcggtg
     3061 gagggatttg aacccccgac cttacgggta tgaaccgtac gctctagcca actaagctac
     3121 accgccagct atatattcaa tttgaactta tacaaaaatg gtggatccta acgggatcga
     3181 accgtcgacc tcctgcttgc aaaacaggcg ctctcccagc tgagctaagg acccacaaaa
     3241 aacgaaacaa aaataaatct atacaattgg tgcctaaggc gggaatcgaa cccgcacagt
     3301 attgctacca caggatttta agtcctgcgc gtctacctat tccgccactc aggctacaat
     3361 ggtgtctccg ggacgattcg aacgtctgac ccactgatta aaagtcagtt gctctacctg
     3421 ctgagctacg gagacaaatg gtgccggctg caggacttga acccgcaacc tattgattac
     3481 aagtcaattg ctctaccagt tgagctaagc cggcaaacaa tggtggagaa tgtagggctc
     3541 gaacctacga ccctctgctt gtaaggcaga tgctctccca actgagctaa tcctccacag
     3601 ttaataaaat attaaaatgt ttttatcctt taatttaata gaaaggagtt tggcgatttc
     3661 ctacttttgc gggctatata accaactatc atcagcgtta aagtgcttaa cttctgtgtt
     3721 cgagatggaa acaggtgtgt ccactttgct attaccacca aactcttctt tcaaaattga
     3781 atagtactta tcttctttgt aggttaagtc ctcgacctat tagtatcagt cagctcaata
     3841 cgtcaccgta cttacactcc tgacctatct acctcatcat ctctaagggg tcttacttat
     3901 tgctaatggg aaatctcatc ttgagggggg cttcatgctt agatgctttc agcgtttatc
     3961 ccttccgcac ttagttaccc agctgtagcc ctggtagact aactggttcg ccagcggtgc
     4021 gtccactctg gtcctctcgt actaagagca gctcctctca aatttcctac gcccacgaca
     4081 gatagggacc gaactgtctc gcgacgttct gaacccagct cgcgtgacgc tttaatgggc
     4141 gaacagccca acccttggga cctgttccag ccccaggatg cgacgagccg acatcgaggt
     4201 gccaaacctc cccgtcgatg t
//
LOCUS       DAOSOL010000106         4211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_21130_length_4211_cov_3.839028, whole genome shotgun sequence.
ACCESSION   DAOSOL010000106 DAOSOL010000000
VERSION     DAOSOL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21130_length_4211_cov_3.839028"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..1897
                     /gene="xylB"
                     /locus_tag="PK171_03610"
     CDS             413..1897
                     /gene="xylB"
                     /locus_tag="PK171_03610"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT05310.1"
                     /translation="MSSFIGIDLGTQSVKVVLYDEAGHLLSLAQRDYPLLTPQIGFAE
                     QDPEEWWKQTFSALQEVLYSVSNREIKAISFSGQMHGLVMLDNNYKSVCPAIIWADQR
                     SEKEVHFIREKLRSRLAPIAGSDIATGFMGASLFWVKNNRPDLYNKTRWVMLPKDYIK
                     IKLGLPPSSDVADAASTLLFDINQRNWSEEIIKELDLKSSLFPPIYESAQIIGQVSSL
                     VQKELEITGDAFVIAGAGDQHCAALGNGIINEGEVLITIGTGGQVFTAIQKPIVDSLL
                     RIHTFCHAVPQTWHLLGASLCAGLSLSWFKKSVLDNNHHTFDYSTLDEEAAWGIAGTN
                     GLIFLPYLIGERTPHRDSLARGAFINLHLTHQRGDMVRAIMEGVSMAIKDSVSIFEEL
                     GIQGKKFIFAGGGAKSRLWRSILSSFLEHPLQTTKVKEEAATGAAILAMVGSGHFNSF
                     NEAVEQMIRYDSPVNPQDQWFPVYRERYQTYRSLYPALKPFFPQ"
     gene            complement(2467..3411)
                     /locus_tag="PK171_03615"
     CDS             complement(2467..3411)
                     /locus_tag="PK171_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05311.1"
                     /translation="MKLPDKATQPTMYFIGVTTEKSSIMKLFPLWAKELGLDGTVLKG
                     IDITIHAPEDDYRKALSFIKDDPLSLGALVTTHKIDLYNACKDLFDFLDPYSQAFGEL
                     SSISKKDGRLVGHAKDPISSGLALEAFVPKNFWHDHGGEVFIMGAGGSARAMAAYLFD
                     QNKGLNVPSKMYVANRSTPRLESMKEIMKKLCPKAPIEYHHCPDVAINDTILKDMKPF
                     SLIVNATGLGKDRPGSPLSNQCEFPQNSLVWEINYRGDLLFMHQALHQKEKKNLTVED
                     GWIYFIHGWTQVIAEVFHIDITEDLFRRIESMSNSFRF"
     gene            complement(3424..3990)
                     /locus_tag="PK171_03620"
     CDS             complement(3424..3990)
                     /locus_tag="PK171_03620"
                     /inference="COORDINATES: protein motif:HMM:NF018288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HOT05312.1"
                     /translation="MKPYNVVINFESGTLDPGKRIIRKVSDVKNMFYDTQAAEELIQK
                     GDPLVYEVIYADVPEEQGHLGHCTTIIYPGKIGNEYFLTKGHYHEKLSTAEIYFCLRG
                     QGRLIMESTDGDCEVLEMFPGSASYIPPNWSHRTMNVGNELLVFYCIFPADAGHDYAT
                     IEEQGYPKLVVEIDGKVTVIDNPKRKNK"
BASE COUNT         1218 a          874 c          803 g         1316 t
ORIGIN      
        1 cggagcagga aaaaattcaa atccccctaa ccccctttgc taaaggggga aacgattcct
       61 gggtaagtat tttcatcctc atctggtgct gctaggcagc atgaaggtct atccttctat
      121 tgtggggttg caatacgttt taataactct tttaaaagtg gccatagaca atgatttttg
      181 gtaaaaaaaa gtccaccttt cctgaaacca ggaaaagatg gacttcccaa attgattttt
      241 attattggct ttctttttct ctaacattcc acaaccccga ataaggatac tcatttcaat
      301 gtttcatgtc aattattttt ccagagaatt tttatcaatt atcttctctt gccagggtta
      361 aaattgaatg ctaatcttaa attgactaat accttttggg aggaggatcg caatgtcttc
      421 ttttattgga atcgacttag ggactcaaag tgttaaagta gttttgtatg atgaggcagg
      481 gcatcttctt tccctggcac agcgggatta tcctctctta accccacaaa taggatttgc
      541 cgaacaagat cccgaagaat ggtggaaaca aaccttttca gcattacagg aagtccttta
      601 ttcagtttca aatcgagaaa tcaaggcgat aagtttttca ggccaaatgc acggtttagt
      661 tatgttggat aacaattata aatccgtttg tccagctatc atctgggcag atcaaagaag
      721 cgaaaaggaa gtacacttca tacgagaaaa attaaggtcc cgtttagccc ctattgccgg
      781 aagcgatatt gctactggtt ttatgggtgc ttctttgttt tgggtcaaaa acaatcgtcc
      841 tgatctttac aataaaactc gttgggttat gttgccaaag gactatatta aaatcaaatt
      901 aggactcccg ccatcatcgg atgtcgccga tgctgcctct acccttctct ttgatatcaa
      961 ccagagaaac tggtcggaag aaattataaa agaacttgat ttaaagtcat ccttattccc
     1021 tcccatttac gaatcagccc aaattatcgg acaagtatca tcattagttc aaaaagaact
     1081 ggaaatcacg ggagatgctt tcgtaatagc cggagccggc gatcaacatt gtgcagcatt
     1141 gggaaatggg attatcaacg aaggagaagt cctcatcaca atcggcactg gtgggcaggt
     1201 ttttactgct atccaaaaac ctattgttga cagcttatta agaattcata ctttttgcca
     1261 cgcagtacct caaacctggc atttattggg agcgtcgctt tgtgctggac tctctttaag
     1321 ctggtttaaa aaatcagttc ttgacaacaa tcatcacacc ttcgattatt caacactgga
     1381 tgaagaagct gcttggggaa tagctggaac caatggcttg atatttctcc cttatttaat
     1441 tggtgaaaga acccctcacc gcgattcatt ggctcgtggt gcttttatca atctccacct
     1501 tacccatcag cgaggagata tggttcgagc tattatggag ggagtaagta tggcaatcaa
     1561 agactcagtc agtatttttg aagaactggg tattcagggg aagaaattca tctttgctgg
     1621 cggaggtgct aaaagccggt tgtggagatc gattctttct tctttccttg aacatcccct
     1681 tcaaaccacc aaagtaaagg aggaagccgc tactggtgca gccatattgg ctatggtagg
     1741 gagtggtcat tttaacagct ttaacgaagc agttgagcaa atgatccgct atgattcacc
     1801 ggtcaatcct caagatcaat ggtttccggt ttatcgagag agatatcaaa cctatcgttc
     1861 tttataccct gcactcaagc ccttctttcc tcaatagata cttatgtcat ctcttgccat
     1921 agatgaaaat gaaaatccaa gatttgacat gtcatggcat gtcgctacct taatcgggca
     1981 taataaatta tgccccaact ttttttaatt cttttgtagg ggcttaattg atcatgccca
     2041 ttccaaatcc gtcattgcga ggagtgtctt gtgcgacgtg gcaatctcat ttagccaatc
     2101 ttttttgtcc ttttccaagg ataagcaata aaaaaattaa aaaataaagg cgccctcccc
     2161 gccgtaagcg ccacccctcc aaggaaggga attgttagat ttattctttc tcatttttgt
     2221 ttgatcactt ttcattgttt tcagcatttt caggatagac cttcatgctg cctagcagca
     2281 ccagatgagg atgaaaatcc aaattcgatg tggcagagct tgtcactaca ttaatcaggc
     2341 gcattacatt acacccctgc agcttttaaa tctttggtaa ggactttatt tatcaagtcc
     2401 ataaattggc tttcaaggaa gtatttttaa atattccaca ttatttaatg actttttggt
     2461 tcaaattcag aaacgaaaac tattggacat actttcaata cgacggaaaa gatcctcagt
     2521 aatatcgata tgaaaaacct cagcaatcac ttgggtccac ccatgtataa agtatatcca
     2581 tccatcttca accgtcagat ttttcttttc cttttgatgg agcgcctggt gcataaagag
     2641 gagatcgcct cgataattaa tctcccatac taagctattt tgaggaaatt cgcattgatt
     2701 agaaagcggt gatcctggtc gatctttccc taagccagtt gcattgacga tgagagaaaa
     2761 gggtttcata tcttttaaaa tggtatcgtt gatagctaca tccggacaat gatggtattc
     2821 aataggtgcc ttcggacaga gctttttcat aatttctttc atcgattcca atcgcggggt
     2881 acttcgattg gcgacgtaca tttttgatgg gacatttaat cctttatttt gatcaaatag
     2941 ataggcggcc atcgctcggg cacttcctcc agcacccatg atgaacacct caccaccgtg
     3001 gtcatgccaa aaatttttcg gcacaaaagc ttctaatgct aagccactcg atattggatc
     3061 tttggcatgt cctactaatc gaccatcttt tttagaaatt gaggaaagct ctccaaaggc
     3121 ctgagaatag gggtcaagaa aatcaaataa atccttgcag gcattataca aatcaatttt
     3181 atgggtggtt accaatgctc ctaaagaaag agggtcgtct tttataaagg aaagtgcttt
     3241 ccggtaatca tcttccggtg catgtatggt aatatcaatt ccttttagaa cggttccatc
     3301 taaacccaac tcttttgccc acaaggggaa aagcttcatg atggaagact tttcagtggt
     3361 aacaccgata aaatacatgg tgggttgtgt agccttatca ggtaatttca atccaattcc
     3421 cccttattta tttttacgtt ttgggttgtc gataactgta accttcccat caatttcaac
     3481 gaccaatttc ggataacctt gttcttcaat agtggcgtaa tcatgaccag catcggcagg
     3541 gaaaatacaa taaaagacta acagctcgtt tccaacattc atggtccgat gcgaccaatt
     3601 gggggggatg taggaagctg aaccaggaaa catttctaaa acttcacaat caccatcggt
     3661 agattccatg atcaacctcc cctgccctcg taaacaaaag tatatttcgg cagtgctgag
     3721 tttctcatga taatgtcctt tggttaggaa atattcattc ccgatttttc ccggataaat
     3781 aatggtcgtg cagtggccta agtgcccctg ctcttcgggt acatcagcat aaatcacttc
     3841 ataaaccagt ggatcgcctt tttggattaa ttcttcagcg gcttgagtat cataaaacat
     3901 gttttttacg tcactgactt tgcgaataat tcgtttacca gggtctagcg tccccgattc
     3961 aaaattgatt acgacattat aaggtttcat aagaatcctt cctttctgaa ctgagtatat
     4021 atgataacgg attctataat catttcataa taactctcga tatttttcaa aaacttcatt
     4081 cattttgtcc atggtttcca aataataagg gataaatttt tcatagactt ttgataattt
     4141 ttcgtctgga taatgaactt ttttcttttc aaccgaattg agagaagttt tttgcaaatc
     4201 ttgaaataca c
//
LOCUS       DAOSOL010000107         4200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_21216_length_4200_cov_3.422437, whole genome shotgun sequence.
ACCESSION   DAOSOL010000107 DAOSOL010000000
VERSION     DAOSOL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21216_length_4200_cov_3.422437"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1287)
                     /gene="fabF"
                     /locus_tag="PK171_03625"
     CDS             complement(46..1287)
                     /gene="fabF"
                     /locus_tag="PK171_03625"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT05313.1"
                     /translation="MDRRVLVTGIGVVSPIGIGKEKFWQALINGESGIDTIKRFDASS
                     YDSQIAGEVKDFNCDDYLPRKDARRMDRFSQFAVSASIMAFQDSQINLDKLNHERSGV
                     ILGSGIGGIFTFEEQHEVLMNRGPQKVSPFFIPMMIVNMGAANVAIQLGLKGPNSCVS
                     TACEASTHAIGEAFRILQRGDADLMVAVGSEASITPLALAGFCSMKALSTYNQEPKKA
                     SRPFDKNRDGFVMAEGAAALILETKESAERRSATIYAELKGYGASCDAYHITAPDPEG
                     EGAARSMLYAIHDAGLRIEDIDYINAHGTSTPLNDKMESLAIKKVFADRAYQLKISST
                     KSMTGHLLGAAGAMEAAATILGIFHHTIHPTINLEEKDEDCDLDYVPQKAQKMEIRNA
                     ISNSFGFGGHNGSLVFGQYKE"
     gene            complement(1302..2285)
                     /locus_tag="PK171_03630"
     CDS             complement(1302..2285)
                     /locus_tag="PK171_03630"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOT05314.1"
                     /translation="MNNRPVKILGVGAGVPQKVITNFDLEKIVDTSDEWIVSRSGIRE
                     RRIASENETTSMLAIEAARIALKKAQVKSSDLDLIIVATVTPDMLFPATACILQRELG
                     AEKAACFDLEAGCTSFVYALSIAEKYLSAGGGNFALVVGAETLSKILDWEDRATCVLF
                     GDGAGAAVLGLGEKPGIISTHLGADGGGANYIEVPAGISRMPASIDTVQNRLHYIKMA
                     GNEVFKFAVKIMEEASLKVLEKGKIKIEDVNLFIPHQANIRIIKSAAKRLGIVEDRIF
                     VNIHKYANTSSASIPLALFEAVQENRIQEGDIILLVGFGAGLTWGSALIRW"
     gene            complement(2282..2722)
                     /gene="fabZ"
                     /locus_tag="PK171_03635"
     CDS             complement(2282..2722)
                     /gene="fabZ"
                     /locus_tag="PK171_03635"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOT05315.1"
                     /translation="MDLDNRMIQRILPHRFPFLLVDRVTIEEMTAVGIKNVTINEWFF
                     QGHFPGLPTMPGVLIIEAMAQVGATMMMNLDEYHDCVPYFTTIDRVKIRKPVVPGDQL
                     IIKVELLKIRKRMGKLNCIAKVNETIVAEGEIGFSLVEKKEEVI"
     gene            2890..3993
                     /locus_tag="PK171_03640"
     CDS             2890..3993
                     /locus_tag="PK171_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOT05316.1"
                     /translation="MLRGLRKNLDVIIIIIVVAFVFTIFYGTFSRRSQNPSQSAAAAA
                     TVNNTVITIYDLENQFRNFISQFDNKYLNELDEQGINYLKRLTLESMINNELLHQEAK
                     SRKIKVNNNDINTRLNEIKANFPSDREFQNYLQYNGIRINDMRESIKRDLMITHLTNS
                     LYESIVIPPEDINNYYEENKSLFSTPTQYHLNQMTFPSQEEAEKVYKRVSLGEDFSNL
                     AKLNSIDTYASQGGDAGWISENALPNEAKDSIIELKDKLGSVTPIVKVGNNYQFFKLI
                     KTKPAEEKTLEESQEEIKVILENEQKRVKLEHLVAEIRNKSKIEYSESILASGIVSEP
                     SPTEPLQSENPAAPLPESSEPTEIPDITSTPNP"
BASE COUNT         1303 a          923 c          706 g         1268 t
ORIGIN      
        1 cgatgagata attaatacca ataacccacc atccagacat aataattact ccttatattg
       61 tccaaaaacc aaagaaccat tatgtcctcc aaatccaaat gaattagaaa ttgcattacg
      121 aatttccatt ttttgagctt tctggggaac ataatctaaa tcacaatctt cgtctttctc
      181 ttcaagattg atagttggat gaatggtatg atgaaatatt cctaaaatag ttgctgctgc
      241 ttccatggct cctgctgctc ctaaaagatg accggtcatg gatttggttg aactaatttt
      301 tagttgatac gctctatcgg caaaaacctt tttaattgcc agtgattcca tcttatcgtt
      361 taacggtgtt gatgttccat gagcattgat ataatcgata tcctcaatcc gaagtccggc
      421 gtcatgaatg gcataaagca tagaacgagc cgctccttca ccttctggat cgggtgcagt
      481 tatgtggtaa gcatcacagc tggcaccata tcctttcaac tcagcataga tcgtagcact
      541 tctccgttct gctgattctt tcgtttctaa aatcaacgct gctgccccct cggccataac
      601 aaaaccatcc cgatttttat caaagggtcg tgaagctttt tttggttctt gattataggt
      661 agaaagggct ttcatcgaac aaaaaccggc taaggcaagt ggtgtaattg atgcttccga
      721 accaactgcc accattaagt cagcatcacc tcgttgtaat attcggaagg cttctccaat
      781 cgcatgagtg gatgcctcgc aggcagttga tacgcagcta tttgggccct ttaaccccaa
      841 ctgtatggct acgtttgctg ctcccatatt aacaatcatc atcggaataa aaaaaggact
      901 aaccttttgt ggaccacgat tcattaatac ctcatgctgt tcttcaaaag taaaaattcc
      961 accgatcccc gaacccaata taacacccga acgctcgtga ttgagcttat ctaaatttat
     1021 ttgtgaatcc tgaaaagcca tgatggaagc acttacagca aattgagaaa agcgatccat
     1081 tctgcgagcg tctttgcgag gtaaataatc atcacaatta aagtctttta cctctcccgc
     1141 tatttggcta tcatatgaac tggcatcaaa tctcttaatg gtatcaattc ccgattctcc
     1201 attaatcagg gcttgccaaa atttttcttt tccaattcct attgggctaa ctaccccaat
     1261 tccggtaacc aagactcttc tatccaaagt tgaaacctcc cttaccatcg aataagagct
     1321 gaaccccaag tcaagcctgc accaaaacca actaacaata taatgtctcc ttcctggatt
     1381 cgattttctt gaactgcttc aaagagtgcc aaaggtatcg aggcagagga agtgtttgca
     1441 tatttatgaa tgtttacaaa gatacgatct tcgacaatgc caagacgttt cgcagctgat
     1501 ttgatgatcc gtatattagc ctgatggggg ataaataaat tgacatcttc aatctttatc
     1561 ttcccttttt ctaaaacttt cagcgaagcc tcttccataa tttttacggc aaatttaaag
     1621 acttcatttc ctgccatttt tatataatgg agtcgatttt gaacggtatc aattgaggct
     1681 ggcatacggg aaataccggc tggaacttca atatagttag ctcctcctcc atcagcacct
     1741 aaatgggttg aaataatacc cggtttttct cccagaccta acacagctgc tccggctcca
     1801 tcaccaaata gcacacaggt cgctctatct tcccaatcga gaattttcga taaagtttcg
     1861 gctccaacca ctaaagcgaa attcccaccc ccggcggaaa gatacttttc tgcaatggaa
     1921 agagcataaa caaaactagt acagccagct tccaggtcaa aacaagcagc tttttctgca
     1981 cccaattcac gttggagaat acaggccgtc gctgggaaaa gcatatcggg agtgacagta
     2041 gcaactataa tcaaatcaag gtcagatgat tttacctggg cttttttcag agctattcgt
     2101 gccgcttcaa ttgccaacat agatgtggtt tcattttccg aagcaattct ccgctctcgt
     2161 atgccgcttc gactgacgat ccattcatca ctggtgtcca ctattttttc caaatcaaaa
     2221 ttggttataa ctttttgggg aacacctgct ccaactccca gtattttcac cggtcgatta
     2281 ttcaaatcac ttcctccttc ttttcaacga gtgaaaaacc tatttctccc tcggctacta
     2341 tagtttcatt gaccttcgct atgcagttta acttacccat tctcttacga atcttaagaa
     2401 gctctacctt aataattaat tggtcgcccg ggacaaccgg ttttctaatt ttcaccctat
     2461 caatcgtggt aaaataagga acacaatcat gatactcatc caaattcatc atcattgtgg
     2521 cgcccacctg agccatagct tcgataatga gcacacctgg catggttggt aatcccggaa
     2581 aatgaccttg aaaaaaccat tcattgattg tcacgttttt aattccaacg gcagtcattt
     2641 cttcaatagt aactctatct actaataaaa atggaaaacg atgaggcaag attctctgga
     2701 tcattcgatt atccaagtcc attttttaat acctcccctt ttttttaaaa gtcttctatt
     2761 caatcctcta tcagttcgtc gtgttcacgc tggactattg agagaatttt gggtaaattg
     2821 acataaaagg ataactggag tagtatttta ccaaaaataa caataattgt ctcaggggtg
     2881 ggagaatata tgctgagagg acttcgcaaa aatctcgatg ttatcatcat aatcattgtg
     2941 gttgcctttg tttttactat tttttatggt actttcagtc gccgatcaca aaatccttca
     3001 caaagtgccg cagcggcggc aactgttaac aataccgtta ttactattta tgaccttgaa
     3061 aatcaattcc gtaatttcat ctctcagttt gataacaaat atctcaacga acttgacgaa
     3121 cagggaatta attaccttaa aagactcacc ttagaaagta tgataaacaa tgaactttta
     3181 caccaggaag caaaatcacg aaaaattaaa gtgaataata atgatattaa taccaggcta
     3241 aatgaaatta aagccaattt tccttctgat cgggagtttc aaaattactt acaatacaat
     3301 ggaatccgaa taaatgatat gagagagtcc atcaaaagag atttgatgat cacccacctt
     3361 actaattcat tatacgaaag tattgttatc ccgcctgaag acattaacaa ttattacgaa
     3421 gaaaataaaa gtttgttttc aacgccaacc caatatcacc tcaatcagat gacatttcca
     3481 tctcaagaag aagccgagaa agtttataaa agagtcagtt tgggcgaaga tttttccaat
     3541 ttagctaagt tgaattcaat tgacacctat gcttcccaag gtggagatgc tggttggata
     3601 tcagaaaatg cccttcccaa cgaagctaaa gattctatta ttgaattgaa agataaattg
     3661 ggtagcgtta ctccaattgt gaaagtcgga aacaattatc aattctttaa acttattaaa
     3721 accaaaccag ctgaggagaa aacattggaa gagtctcaag aagaaataaa agtaattctc
     3781 gaaaacgagc aaaaaagagt aaaattagag catttagttg ctgaaattcg caacaaatcc
     3841 aaaatagaat attctgagag cattttagcc tcaggtatag taagtgaacc atcgccgact
     3901 gagccgctgc aatctgagaa tccggcagcg ccattgcctg aatcatccga accaaccgaa
     3961 atacccgata taacatcaac accaaatcct tagaggattt tgtttctatt gattattcat
     4021 cgatattcat caaaaatgaa aacagcccta tcttttcttt tgatcgtttt gattatgggt
     4081 ttgtttattg tatttattcc ccgggttttt tcttctacac ctcaaaacgt gttttttctt
     4141 aatcaaagtt ttagcaatat tgccaaaaat gatattattc cttatctcca actcatggaa
//
LOCUS       DAOSOL010000108         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_21294_length_4190_cov_3.509553, whole genome shotgun sequence.
ACCESSION   DAOSOL010000108 DAOSOL010000000
VERSION     DAOSOL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21294_length_4190_cov_3.509553"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1111
                     /locus_tag="PK171_03645"
     CDS             155..1111
                     /locus_tag="PK171_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOT05317.1"
                     /translation="MLTLDKKLEMYRQMITIRLFEEQAELLYIEGKIFGTFHLYVGEE
                     AVAVGACAALQPDDFITSTHRGHGHCIAKGADVKRMMAELMAKDTGYCHGVGGSMHIA
                     DVEKGNLGANGVVGGGIPIATGAALGCKLQKNGKVVICFFGDGASNTGTFHESINMGS
                     AFKLPVVYLCENNQYAMSTSVKYSVAIDDISERSKAYGIPGVTINGNKVLEVYEAVSL
                     AIERAREGKGPTLIEAKTYRWKGHSKSDKNVYRTKEEIKSWKARCPIQSFRQYLINDE
                     GIKEDILGNIDVEVEKLIQESVQYAEQSPEPDLEAVRRMVYA"
     gene            1104..2087
                     /locus_tag="PK171_03650"
     CDS             1104..2087
                     /locus_tag="PK171_03650"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOT05318.1"
                     /translation="MPDRVITYSEAIREALREELKNDPRVFLMGEDIGIYGGAFAVTL
                     GLYEEFGPERVIDTPISEAAIIGAAAGASLVGMRTVAEIQFSDFIAIGMDQLVNQAAK
                     IRFMFGGKAAVPMVVRAPIGSGTGAAAQHSQSQEAWYAHVPGLKVVLPSNPADAKGLL
                     KSAIRDDNPVIFLEHKLLYKTKGVVPEEEYLIPIGKADVKRSGADLTIIGYSNLIPKS
                     LQAAEKLAQEGIDVEVLDLRSLRPLDTEAIAQSIQKTHRALVVYEAPQLGGFGAEVAA
                     FIGEKCFDDLDSPVMRLGGLEMPPPYNPRLEKQLVPQVEDIVNGVRRLMKG"
     gene            2091..3044
                     /locus_tag="PK171_03655"
     CDS             2091..3044
                     /locus_tag="PK171_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOT05319.1"
                     /translation="MKYDAFHQDLMREVAKLYYYEDLTQDQIGEIVGLSRQKVWRVLK
                     KAKEEGIVQIKIIEPSDKELENENQLKEKFGLKEVKITLSFQENDKILLKRVAQVAAS
                     YLRNRLDPYSTLGVAYGKTIFEIIHYLTPKKIPGLRVIQIMGGYGKLKGDVMAVELAR
                     RIAENFDGQVVYLMAPAFAKDQATRDAILSNEGVFKVLDMSKKVDIALVGIGGVLETS
                     TLLDTGDLYDHEIEELRKKKVVGNICGNFYDEMGAIIETLADKRRISISLQDLKKIPL
                     VIGVAGGKNKFLPILGALKGRFIQALITDDLTAKLLINHKE"
     gene            3077..3769
                     /locus_tag="PK171_03660"
     CDS             3077..3769
                     /locus_tag="PK171_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOT05320.1"
                     /translation="MLNDLKKKVYDANIELQKKGLVIYTWGNASEIDQEKKLVVIKPS
                     GVPYEKLTPEMMVVVDLEGQIIEGDYRPSVDTKIHLDLYKAFPELGGIVHTHSTYATA
                     WAQAQKSIPCLGGTHADYFFGEVPCTRLLTEQEVEESFEGDTGKVIAECFATKKPLDV
                     PGVLVASHGPFSWGKDSHQAVFNAVVLEELARMALFTIQINPEIQSIPQSLLEKRYLR
                     KHGKDATYGQII"
BASE COUNT         1347 a          642 c          983 g         1218 t
ORIGIN      
        1 tgaaaaccag ggttcgacat gccagagtag gtcgctacat taattaaaga atgggcacaa
       61 tacattgtgc ccctgcgttt tttaattctt tagtaggggg cttgatttat catgcccgtg
      121 tattttcagg atagaaaagg aggtaaattc caatttgctt actcttgata aaaaattaga
      181 aatgtatcgg caaatgatta cgattcgttt gttcgaagaa caagctgaat tgttgtacat
      241 tgaaggaaag atttttggga ctttccatct ttatgttggt gaagaggcgg tagcggttgg
      301 tgcttgtgcc gctttacaac ctgacgattt tattacctct acccatcgag gccatgggca
      361 ttgtattgca aaaggagccg atgtaaaacg catgatggca gaattaatgg ccaaagatac
      421 tggatattgc catggagttg gaggttcgat gcatattgcc gatgtagaaa aaggaaattt
      481 aggggccaac ggtgtggtag gtggtggtat tcctattgct accggtgcag cattagggtg
      541 taagctgcaa aaaaatggga aggtggttat ctgttttttc ggagacggag cctcaaacac
      601 tggtactttc catgaatcga taaatatggg atctgctttc aaacttccag tcgtgtattt
      661 atgtgaaaat aatcaatatg caatgtcaac ttcggttaaa tattctgtgg cgatagatga
      721 tatctccgag cgttccaaag cttatgggat tcctggagtg accataaatg gaaataaagt
      781 tcttgaggtg tatgaagcag taagcctggc tattgaacga gctcgggaag ggaaagggcc
      841 aactcttatt gaagcgaaaa cctatcgctg gaaaggtcat tctaaaagtg ataaaaatgt
      901 ttatcggact aaagaagaga taaaaagttg gaaagctcgt tgtcctattc aatcttttcg
      961 ccagtatctt atcaacgatg aaggtattaa agaagatatt ttaggaaaca tcgatgtcga
     1021 agttgaaaag cttatccagg aatcggttca atatgctgag caaagtccag aaccggattt
     1081 agaagcggtg aggaggatgg tttatgcctg atcgagtgat tacttattct gaagccatcc
     1141 gggaagcatt gcgtgaagag ctaaaaaatg atcctcgggt ttttttaatg ggggaagata
     1201 ttggtattta tggtggtgct tttgccgtaa cccttggttt atatgaagag tttggtccag
     1261 aacgggtcat tgatactcca atttcggaag cagccataat tggtgctgct gccggggctt
     1321 ctttagtggg aatgcgaaca gtcgccgaaa tccaattttc agattttatt gccattggta
     1381 tggatcaatt ggtcaaccag gcggcaaaaa ttcgttttat gtttggtgga aaagcggcag
     1441 tacctatggt ggttagagct cccattggtt cgggtactgg agcagcagct caacattctc
     1501 aaagccagga agcctggtat gctcacgtgc cgggattaaa agtagtttta ccttcaaatc
     1561 ccgctgatgc caaagggctt ttaaaaagtg ctatacgaga tgataatccg gttatttttt
     1621 tagagcataa attgttatat aaaaccaagg gagtggttcc agaagaagaa tatttaattc
     1681 caatagggaa ggcagatgtc aaaagatctg gggctgatct cactattatt ggttattcta
     1741 atctcattcc caaaagttta caagcagcgg aaaaattggc tcaagaaggg attgatgtag
     1801 aggttttaga tcttcgttcc cttcgcccac tcgataccga agccattgcc caatcaatac
     1861 aaaagaccca tcgagcattg gtagtttatg aagctcctca attgggaggg tttggtgctg
     1921 aagtggcagc atttattgga gagaaatgtt ttgatgacct tgattctcca gttatgcgct
     1981 tgggaggtct tgaaatgcct ccaccctata atcctcgttt agaaaagcaa ttagtacctc
     2041 aagtggaaga tatcgttaat ggggttagaa ggttaatgaa aggataagaa atgaagtacg
     2101 atgcctttca tcaggatctg atgcgagaag tggctaaatt atattactac gaagatttaa
     2161 ctcaagatca aataggagaa attgttggcc tttctcgtca aaaagtttgg agagtgctta
     2221 aaaaagctaa agaagagggt atagtccaaa ttaaaataat agaaccctcc gataaggaat
     2281 tggaaaatga aaatcagctt aaggaaaaat ttggtttaaa ggaagttaag attacactat
     2341 cctttcaaga aaatgacaaa atacttttaa aaagagttgc ccaagttgct gcctcttatt
     2401 taagaaatcg cttagatccc tattcaacct taggggtggc ttatggaaaa accatatttg
     2461 aaataatcca ctatctcact cctaaaaaaa tacccggttt aagagtgatt caaattatgg
     2521 ggggttatgg aaaattaaaa ggggacgtca tggcagttga attagcccgt cggattgctg
     2581 aaaattttga tggtcaggtt gtttatttga tggctccggc ttttgctaag gatcaagcca
     2641 ctcgagatgc gattttaagt aatgaaggcg tttttaaagt tctcgatatg tcaaaaaaag
     2701 ttgatatagc tttggtagga attggaggag ttttggagac ttcaaccctt cttgataccg
     2761 gggatttgta tgatcatgaa attgaagaat tacgaaagaa aaaagtcgtt gggaatattt
     2821 gtggcaattt ttacgatgaa atgggcgcta ttattgaaac cttagctgat aaaagaagaa
     2881 tcagtattag tcttcaagat ttaaaaaaaa taccattggt aattggggtg gcggggggca
     2941 agaataagtt tcttcctatt ctgggagcac tcaagggaag attcattcaa gctttgataa
     3001 ccgatgacct aactgccaaa ctattgatta accataaaga ataaatgaat tgtcactgag
     3061 aaagcagggt gagattatgc taaatgattt gaaaaaaaag gtttatgatg cgaatattga
     3121 acttcagaaa aaaggtttgg tgatctatac ctggggaaat gccagtgaaa tagatcagga
     3181 aaaaaaatta gtcgttatta agcccagtgg tgtcccctat gaaaaactga ctccagaaat
     3241 gatggtggtt gtcgatcttg aaggacagat aattgaagga gattatcgac catcagtgga
     3301 cactaaaatt catttggact tatataaggc ttttcccgaa ttgggaggaa tcgttcatac
     3361 tcactcaacc tatgcaacgg cttgggctca ggcgcaaaag agtattccat gtttaggtgg
     3421 aactcatgct gattattttt ttggcgaggt cccttgcacc aggctactta ccgaacaaga
     3481 ggttgaagaa tcttttgaag gagataccgg aaaagtgatc gccgaatgtt ttgcgaccaa
     3541 aaaacccctt gatgttcccg gagtattggt ggccagccat ggaccattct catgggggaa
     3601 agatagtcac caagcagttt ttaatgcggt agttcttgaa gaattggctc gtatggcatt
     3661 gtttaccata caaatcaatc cagaaattca aagcatacca cagagcttgt tagagaaacg
     3721 ctatttgaga aagcatggga aagatgcgac ctatgggcaa attatataaa tagatactta
     3781 ttttgatgaa attcttaaat tgaagttaaa taactggcag aaactacaaa ttgtaatcta
     3841 gtttatataa tgaaatatta tgatttaacc taatataaaa tattgtattg aataatacta
     3901 ataataatta ttaaagaaaa aagagagata ttttaatatg aagtgaggtg ttattatata
     3961 aatctaagta attttcaagc aaggggaaag tgtgattatg ataaacttga agtctcatat
     4021 gattacccac aaaatcgttt tgtaggatgg tttttgggaa atccgggaat gaattttatt
     4081 gaaggagtac aatgggaaaa tgaaaaagag gctcttcaag tgggacactt tcaattggga
     4141 tttaaatcga tcgaaagtga gcataaaggt caaagagagg tgatattagg
//
LOCUS       DAOSOL010000109         4128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_21705_length_4128_cov_3.724527, whole genome shotgun sequence.
ACCESSION   DAOSOL010000109 DAOSOL010000000
VERSION     DAOSOL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21705_length_4128_cov_3.724527"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PK171_03665"
     CDS             complement(<1..993)
                     /locus_tag="PK171_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05321.1"
                     /translation="MSKKAVIIGAGALALGFLGERLALDYEITFADKNIKTPFLKELQ
                     NQQQYILNICHLDGIESVSIQGRFNVFNLDQEKEKNDFYKVITDADLIFTTVGNQNLP
                     IVISQIAPILNEQMRGNCILLCENGRNLAQIYQKFFKASKVVDTVMSRMCRFTTQEES
                     GYGYKPLWKNLPDRLIVEAYDYLPLNVAACSSEQFSPVFTLIEPDIFSTWEDIKFFAH
                     NGTHAFIAYHAFLDGIKYFSDVDHKLKEKAEKMLFNEIIPALHRAHPDFPLRDLKDYG
                     ENLLERIYNPFFHDSIERGIRGILEKLKPNERLVSGLRFIHQQGVYPIHYSSTIR"
     gene            complement(990..2198)
                     /locus_tag="PK171_03670"
     CDS             complement(990..2198)
                     /locus_tag="PK171_03670"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT05322.1"
                     /translation="MSSYHSKAFVYHGHPEQVTLETLDLDCGPNDLIVKVLMCARCGT
                     DKSIFLHGHKNVDPYAPVVLGHELIGTIVEVGNNVKNCNEGIGYAQGKKLSPDYLSFS
                     VVERVTFQSRIARYKNGLMLIPKPIANLSFQINGGYAQYMRVPEDMIRSESVIRVPEI
                     VSDEEACLIEPAACALESIYATPHPVGVDKEGRHIYKAGIQSGGKTCIIGSGTVSMLY
                     ASLAILEGASKVVMVVRSEKKEKLIHQILKNQVNVYVVPQLINSTIQERLLQEEKIVH
                     DLEEMTRGYLFDDVVCACSDPNAQRLMLKLYTREGYGVGACFGGTHSLVDQVDIDQNH
                     YRCAKTIGTSGCSTDAMKAVLQWLYEGKLSFKGFTSSRKFTFDDSPQEFFTTSADGLK
                     PVLYPWESRL"
     gene            complement(2301..2999)
                     /gene="deoC"
                     /locus_tag="PK171_03675"
     CDS             complement(2301..2999)
                     /gene="deoC"
                     /locus_tag="PK171_03675"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT05323.1"
                     /translation="MITKEQFAAMQDHSILEPYATENEIRKRCEETIQYGFAAVYVNP
                     CWVSFAKKIIGDKAKVGTVVGFPHGANTTKIKIAEALEVIDNGADALDLVINISKLKS
                     KDYDYVENELKQFVKAVKDKKPSVLVKIIIETCYLNHDEKVKACEIVARSGADYIKTS
                     TGCGPAGCKVSDIRIMKRIVGDKMKIKGADRISSVEDALSIIEEGASLLGESHAIQWL
                     ADWDKQLWSKDYKR"
     gene            complement(3037..>4128)
                     /locus_tag="PK171_03680"
     CDS             complement(3037..>4128)
                     /locus_tag="PK171_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT05324.1"
                     /translation="KTELKPFGNTLLIVAQKKIEEMGYLKRVTDQLTDGHFKLYFWSS
                     IHDNPVVSDVDDAVESLKSIKFDAIIALGGGSTIDVAKALSVTACTGGLAWDYVYSSA
                     KRTVSQTIPVIAIPTTSGTGSHMTKYSVLTNPQQGIKSTIVTEKIFPKVAIVDPTLMV
                     SLPQYMTAVTGFDALSHAVESYVCINANRMTDMICEKAIQTIAKALPQVINNLNDLIL
                     REDMAWADTLAGIGIAYSGSTLPHAMGQPISGRYPKIRHGETLAAIYPSIVGFSYPGN
                     VEKFDRIVRWIAEGIKMEIENIDAELASKVFSEFLKRIGLSIRLSDFGLEEKNIHDFI
                     DPTLKCGDVYTNPVVATEQDIIELFKLSL"
BASE COUNT         1284 a          854 c          700 g         1290 t
ORIGIN      
        1 ccgtatagtg ctggaataat gaatcggata cactccctgt tgatgaataa atctcaatcc
       61 actcactaac cgctcgtttg gctttaattt ctccaatatc ccacgaatac ctctttcaat
      121 tgaatcatga aagaatggat tgtatattct ctctaaaaga ttttcgccat aatctttcaa
      181 atcacgtaaa ggaaaatcag ggtgagctcg atgtaaagcc gggatgattt cgttaaacag
      241 cattttttca gctttttcct ttaatttatg atcaacatcg gaaaaatatt ttattccatc
      301 tagaaaagca tgataggcaa taaaagcatg agttccgtta tgggcgaaga atttaatatc
      361 ttcccaggtt gaaaaaatat caggttcaat cagagtgaaa accggagaaa attgttcaga
      421 tgagcatgct gcaacattga gcggaaggta gtcataagct tcaactatca acctgtcagg
      481 aaggtttttc caaagtggtt tataaccata accactttct tcctgtgtgg taaagcggca
      541 catccgagac atcacagtat caaccacctt actggcttta aaaaattttt gatagatttg
      601 agccaagtta cgtccgtttt cacataaaag gatacaattc cccctcattt gttcgttgag
      661 tattggggca atctgggata taacgatcgg taaattctga tttcctaccg tggtaaaaat
      721 cagatcggca tcagtaatta ctttataaaa gtcatttttt tctttttctt ggtcaagatt
      781 aaagacatta aatcgtcctt gaatcgagac agattctatg ccatcaagat ggcagatgtt
      841 gagaatatac tgctgttgat tttgaagttc tttcaaaaat ggagttttta tgttcttatc
      901 agcaaaagtg atttcataat ccagagctaa tcgttcacct aaaaaaccta atgccaaggc
      961 tccggctcca ataataactg cctttttact cataatcggg attcccaggg atagagaacc
     1021 ggtttaagcc catcagctga tgtggtaaaa aattcttgag gggagtcatc aaaggtaaat
     1081 ttcctggatg aagtaaagcc tttgaaagat aattttcctt cataaagcca ttgtaatacc
     1141 gctttcatcg catcggttga acaaccactg gttccaatgg ttttggcaca acgataatgg
     1201 ttctgatcaa tgtcgacttg atcgaccaaa gaatgggttc ccccaaaaca agctccgact
     1261 ccataccctt ctcgggtata aagcttaagc ataagccgct gggcatttgg atcagaacag
     1321 gcacacacta catcatcgaa taaatatcct ctggtcattt cttccaaatc atgaacgatt
     1381 ttttcttctt gtaaaagtct ttcttgaata gtggaattta tgagctgagg aacaacataa
     1441 acattgactt ggttttttaa aatttgatga atgagttttt cttttttttc agatcgaacc
     1501 accatgacca ctttactcgc accttctaat attgccaatg aagcataaag catactgacg
     1561 gttcccgaac cgataatgca ggttttccca ccagattgta taccggcttt atagatatgc
     1621 ctcccctcct tgtctactcc aactggatga ggagtggcat aaatcgactc caaggcacaa
     1681 gctgctggtt ctatcaaaca ggcttcttca tcagaaacta tctcaggaac tcgaatcact
     1741 gactctgaac gtatcatatc ttcaggaact ctcatatatt gagcatatcc gccattaatt
     1801 tgaaaagaaa ggttggctat cggtttggga ataagcatca agccattttt atagcgagct
     1861 atgcgggatt ggaaggtcac tctctcaaca acagaaaaac tgagataatc aggggataat
     1921 ttttttcctt gagcatatcc tatcccttca ttacaatttt taacattatt accaacctca
     1981 acaatagttc caatcaactc atgaccaaga acgacgggag cataagggtc gacgtttttg
     2041 tgaccatgta agaaaattga tttatcggtt ccacaacggg cacacatcaa aaccttgaca
     2101 attaaatcat ttggaccaca atccaagtca agggtttcca acgtaacctg ctcgggatgg
     2161 ccatggtaaa caaatgcctt agaatgatag gaagacatat tcctctcctt ttttaaaggt
     2221 aaatattggc aatacaaaac attgtattag aaacaaaaaa ggactccata tattttaaaa
     2281 ggagtcctta attaaataaa tcatctctta taatcttttg accataactg tttatcccaa
     2341 tcagcaagcc attgaatagc atggctttca cctaaaagag acgctccttc ctcaatgatc
     2401 gacagcgcat cttcaaccga gctaattcta tcggctccct tgattttcat cttatctcct
     2461 actatgcgtt tcataatccg aatatcgctt accttacaac cagcagggcc acatcccgtg
     2521 gaggttttaa tgtaatcagc tcctgaacga gcaacaattt cacaagcctt gaccttttca
     2581 tcatgattga gataacaggt ctctatgatg attttcacca aaacactcgg ttttttatct
     2641 ttaactgctt taacaaattg cttcaattca ttttccacgt agtcataatc ttttgatttt
     2701 agttttgaaa tgttaataac taaatcaaga gcatccgctc cattgtcaat tacttcaaga
     2761 gcttcagcga tttttatctt ggtcgtattt gcaccatgtg ggaaaccaac tactgtaccc
     2821 acttttgctt tgtctccaat aattttttta gcaaaggaaa cccagcatgg gttgacataa
     2881 actgctgcaa atccatattg aatagtttct tcacatcttt ttcgtatttc attttcagta
     2941 gcataaggtt ctaatattga atgatcttgc attgcagcaa attgttcctt ggttatcatt
     3001 aatattacct ccaataatta ataatatttt gctcaactac aatgataatt taaaaagttc
     3061 gataatatcc tgctcagtag ctaccactgg attagtataa acatcaccgc attttaacgt
     3121 tggatcgata aaatcatgaa tgtttttttc ttccaatcca aaatccgaca aacgaattga
     3181 taatccaatc ctttttagaa attcactaaa aacctttgaa gctaattcag catctatatt
     3241 ttcaatttcc attttgatac cttctgcaat ccaacggact attcgatcaa acttctcaac
     3301 atttcctgga taagaaaaac cgacaatcga aggataaata gccgctaaag tttctccatg
     3361 acgaatcttt ggatatcgtc cagagatagg ttgacccata gcatgaggta aagtgctccc
     3421 cgaataagca attcctatac cagctaaggt atcagcccaa gccatatctt cacgtagaat
     3481 caaatcattt aaattgttga taacctgtgg cagagcttta gcaatggttt gaattgcttt
     3541 ttcacaaatc atgtcggtca tccgattagc attgatacaa acataggatt caactgcatg
     3601 agataaagca tcaaatccag taactgccgt catatattgg ggcaaactta ccattaaggt
     3661 tggatcaacg atggctacct tgggaaaaat tttttctgta acaatagttg atttaatgcc
     3721 ctgttgagga ttggttaata cggaatattt ggtcatatga ctcccagtac cagaggtggt
     3781 aggtatagca ataactggaa tggtttgact tacagttctt tttgctgaag aatagacata
     3841 atcccaagct aatccaccgg tacatgcagt cactgatagg gccttggcta catctatcgt
     3901 gcttccccct cctaaggcaa tgattgcatc aaattttatt gatttgagag attctacagc
     3961 atcatcaaca tcggatacca ccgggttgtc atggatagaa gaccaaaaat ataatttgaa
     4021 gtggccatct gttaattgat ccgtaactct tttcagatat cccatctctt caattttctt
     4081 ttgagctaca attagcagag tattcccaaa aggcttaagc tcagtttt
//
LOCUS       DAOSOL010000110         4083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_22014_length_4083_cov_4.592850, whole genome shotgun sequence.
ACCESSION   DAOSOL010000110 DAOSOL010000000
VERSION     DAOSOL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22014_length_4083_cov_4.592850"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PK171_03685"
     CDS             <1..553
                     /locus_tag="PK171_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOT05325.1"
                     /translation="TNGDQTNTIRDFMARGDLDAYTQLLLQGGVKEETTQIAERPLVG
                     SDYPEEFEEITTTNQIQKGLIIEEEREVNQEKPQVTTAQEAVLATPSPPPSQPKPTPT
                     PVPPQSTPPPTGSFYLQAGAFSSEGNANNMVNTLREMGFGATVEKSANLFKVKVYGFK
                     TKQEASEAIQKIKSKGIDAFIGQ"
     gene            569..922
                     /locus_tag="PK171_03690"
     CDS             569..922
                     /locus_tag="PK171_03690"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT05326.1"
                     /translation="MTLREIKDLLQAEILTGEELLDIEPTSICGSDLLSDVLAFTKEK
                     SLLLTGLTNSQVIRTAEMSDLIGIVFVRGKRPDNATIELATIKKIPLLTTRLPMFESC
                     GRLYQGGARGCSEVK"
     gene            922..1401
                     /locus_tag="PK171_03695"
     CDS             922..1401
                     /locus_tag="PK171_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HOT05327.1"
                     /translation="MDQHQPHIEIKKEITGGDFSNAGGISSELKNILQQAGLPPQFIR
                     RVVIATYEAEMNVVIHAYRGTFSTSIYPDHVQVVLEDEGPGIPDVDLAFQEGFSTAPA
                     HIREMGFGAGYGLSNMKKCSNQIDIQTKVGKGTKVMMVFWLDSSQVGSSAPDQKEMP"
     gene            1454..2803
                     /locus_tag="PK171_03700"
     CDS             1454..2803
                     /locus_tag="PK171_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT05328.1"
                     /translation="MKVLNPEIFHSVLLDEEKCKGCTHCIRRCPTEAIRVKRGKARID
                     EDRCIDCGECIRTCPNHAKYGQTDSLELLKNFKYTIALPAPAFYAQFKGNLSLGKLLG
                     GLVKLGFNEVFEVAWAAEILADEIDRYLKKTNLDIPIISSACPAVVRLVEVQFPSLID
                     NLLPLDSPLGLAAKIARKQAMEKGFKPEEIGVFFITPCPAKVMEVRRSSGTLERIDVA
                     ISMSSIYPRLINDIDSLPEESSCQLASWKGIGWARSGGEQESLGEGEYMAVDGIHNVI
                     SVFEKIEMGELKKVIYCEAQACVGGCIGGVFAVQNPFIAKVNVNHLIKKHSTPQPPEK
                     HQLEEWRKEKTFQRIHPYEIRDVLKLDNDYRKAIEKYQQIEKILEKLPALDCGACGCP
                     TCRSLAEDIVRGKAQEIDCPFLLREKLCEISKEIFDLASRVPQTMSEEGGKKKIESQ"
     gene            2790..3143
                     /locus_tag="PK171_03705"
     CDS             2790..3143
                     /locus_tag="PK171_03705"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT05329.1"
                     /translation="MKVSDIVKPLEAEVLCEGDLNNSEITGGYCSDLLSDCIANASEG
                     SVWVTIHSHPNIIAVAVLVGMPCIVISNFQEVDPQTIEKAKKEKVTLLRTSLTSYQAV
                     EVLTKIGVHGTKKHA"
     gene            3115..3882
                     /locus_tag="PK171_03710"
     CDS             3115..3882
                     /locus_tag="PK171_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HOT05330.1"
                     /translation="MFTEPKNMPERVYRADLHIHSVLSPCAERDMLPHCIILEALEKH
                     LDIIAITDHNSCENVQVTISLGEQFGVWVIPGMEVETREEIHFLTYFPTYQRILSFYQ
                     VLQQFFQPIPLDENLWGEEWVIGAAGQIVEKKKELLTFPLSLTVEELYQLVVIQDGII
                     IPSHVDRSVYSIIGVLGFLPPYPPFPVLEISSALATSNARVKLKLNNCRLVRFSDAHA
                     LPQVGLVYTEFRMDRPCWVEFKKAINQMEGRDFFPQE"
BASE COUNT         1212 a          829 c          920 g         1122 t
ORIGIN      
        1 caccaacgga gatcagacaa acacaatccg cgattttatg gcgcggggag atttggatgc
       61 ctatactcag cttctccttc agggaggagt caaagaagaa acaactcaaa tagcggaaag
      121 acctttagtt ggaagcgatt atcctgaaga atttgaagaa ataactacga ccaatcaaat
      181 tcaaaagggg ttgataattg aagaagagag agaagtgaac caagaaaagc cacaagtaac
      241 tactgctcag gaagcagttt tagcaactcc atcaccacct ccatctcaac ccaaaccaac
      301 gccgacaccg gttcctcccc agtcgacacc accaccgacc gggagttttt atttacaagc
      361 gggggcattt tccagtgaag gcaatgccaa taatatggta aataccctca gagagatggg
      421 gtttggggca accgttgaaa aatcagctaa tttgtttaag gtaaaagttt acggttttaa
      481 aaccaaacaa gaggcatctg aagcgataca aaaaatcaag tcgaaaggaa ttgatgcctt
      541 tatcggtcaa taaggagaga ggtttccaat gaccctacgt gaaatcaaag accttctaca
      601 ggctgaaata ttaactggag aggaattatt agatatcgaa ccgacctcga tttgcgggag
      661 cgatctttta agcgatgtgc tggctttcac caaagaaaaa tcactactac ttaccggcct
      721 taccaattca caagttatcc ggacagctga aatgagcgat ctgattggta tcgtttttgt
      781 tcgaggcaaa agaccggata atgcaaccat cgaattagca accatcaaaa aaatacccct
      841 tttgacaact cggctgccca tgtttgaatc ctgtgggaga ttgtatcagg gaggagctcg
      901 agggtgtagc gaagtaaagt aatggaccaa catcaacccc acattgaaat caaaaaggaa
      961 attactggag gtgatttttc aaacgctgga ggaatatcca gcgaactgaa aaatatcctc
     1021 caacaagctg gtttaccgcc gcaatttatc cgacgggtgg taatcgctac ttatgaagcc
     1081 gagatgaatg tggtaatcca tgcctatcgg ggaacatttt ccacatcaat ttatcctgac
     1141 catgtacaag tggttcttga agacgaagga ccggggattc ctgatgttga cttagccttt
     1201 caagagggat tttcaacggc accagcccat attcgagaaa tgggttttgg agcagggtat
     1261 ggactctcga atatgaagaa gtgttcaaac caaatagaca tccaaaccaa ggttggaaaa
     1321 gggaccaagg taatgatggt tttttggctt gattcatccc aagtaggttc gtctgctcct
     1381 gatcaaaaag aaatgccata aaatttaatt aggggaattc ccacccgttt tttctccgac
     1441 aaaagaaaag agggtgaaag tattgaatcc agaaattttt cattccgttc ttttagatga
     1501 agaaaaatgt aagggttgta ctcattgtat tcgaagatgt ccaaccgagg ctattcgggt
     1561 gaagcgaggg aaggctcgca tcgacgagga tcgttgtatc gattgtggtg agtgcatccg
     1621 aacttgtccc aaccatgcta aatacggcca aaccgattca ctggaattgt taaaaaattt
     1681 taaatatacg atcgctttac cggctccagc tttttatgcc cagtttaaag ggaatctttc
     1741 tctcggcaaa ctattgggtg gcttagttaa gttaggtttt aatgaagtat ttgaagttgc
     1801 ctgggcagct gaaattttag ccgatgaaat tgatcgatac ctgaaaaaaa ccaacctgga
     1861 tattcccatt atctcctcag cctgtccagc ggttgtcagg ttggtggaag ttcaatttcc
     1921 ctcgctgatc gacaatctcc ttcctcttga ctctcccttg ggtttagcag ctaaaatagc
     1981 tcgaaagcaa gccatggaaa aaggcttcaa gcccgaagaa attggggtat tttttattac
     2041 tccctgtccg gctaaggtga tggaggtccg acgctcaagt ggaacgcttg aacgaattga
     2101 tgtcgccatt tccatgtcga gtatttatcc ccgcttaatc aacgatatcg acagtcttcc
     2161 cgaagaatca tcttgccaac tggcttcctg gaagggcatt gggtgggctc gttcaggagg
     2221 agaacaagaa tcccttggcg aaggggaata tatggcagtg gatgggattc ataacgtgat
     2281 ttcagttttt gaaaagatag agatgggaga actcaaaaaa gttatctatt gtgaagccca
     2341 agcctgtgta ggaggctgta ttggaggagt ttttgccgtc cagaatccct ttattgctaa
     2401 agtcaacgtt aaccatctga tcaaaaagca ttccactcct caacctccag aaaaacatca
     2461 gttggaagag tggagaaaag agaaaacttt ccaaagaatt catccttatg aaattcggga
     2521 tgtgttgaag ctggataatg actatcgtaa ggcgattgaa aaataccagc aaattgaaaa
     2581 gatactggaa aaacttcccg cccttgattg tggcgcctgt ggttgcccaa cctgccgatc
     2641 gctcgccgaa gatattgtaa gaggaaaagc ccaagagatc gattgtccct tcttgttacg
     2701 agaaaaacta tgtgaaatat ccaaagaaat ttttgattta gccagtcgcg ttcctcaaac
     2761 catgagtgaa gaaggaggaa aaaagaaaat tgaaagtcag tgacattgta aaaccgctgg
     2821 aagctgaagt tctttgtgag ggagacctca ataatagtga aatcaccgga ggatattgta
     2881 gtgatttact cagtgattgt attgccaatg ccagtgaagg gagtgtctgg gtgaccattc
     2941 actctcatcc caatatcatt gccgttgcgg tattggtagg catgccttgt attgttatta
     3001 gtaattttca agaagttgat ccccagacaa tagagaaagc caaaaaggaa aaagtaaccc
     3061 ttttgcgaac ttccttaacc agctatcaag cagtggaggt gttaaccaag attggtgttc
     3121 acggaaccaa aaaacatgcc tgaaagggtc taccgtgccg atcttcatat ccatagtgta
     3181 ttatctcctt gcgccgaacg ggatatgtta ccccattgta ttatcttgga agccttggaa
     3241 aaacatttag acatcatagc tattactgat cacaactcgt gcgagaatgt gcaagtcact
     3301 atttctttag gtgagcaatt tggggtttgg gtcatcccgg gcatggaggt tgaaacccga
     3361 gaagagatcc attttcttac ttatttcccc acttatcaac ggatactttc cttctatcaa
     3421 gtcctgcagc aatttttcca gccaattcct cttgatgaaa atctttgggg tgaagagtgg
     3481 gtcattggcg ctgccggtca gattgtggaa aagaaaaaag aacttttaac ctttcctctt
     3541 tcgcttaccg ttgaagagct gtatcagttg gtggtaattc aggatggaat cataattcca
     3601 tcccatgtcg atcgaagcgt ttacagcatt attggagtac tgggttttct cccgccttat
     3661 cccccctttc cagttttgga aatttcttcg gctttagcaa cttccaacgc ccgtgtgaag
     3721 ttgaagttaa acaactgtcg cttggtacgt ttttctgatg ctcatgcttt acctcaagtt
     3781 ggtttggttt atactgaatt tcgtatggat cgaccctgtt gggtggagtt taaaaaggca
     3841 attaatcaga tggaaggaag agattttttt cctcaagagt agtttgagta tatattgctc
     3901 ctgagatttt caagccctct ttatttttcc ctttttcctt ttttattttg aaaataaaga
     3961 aaagagaaaa gaattaacgt catcctgagc cctcgctttg tgagggtgtg agaatctcat
     4021 ctgacgccgc cgacgtcatc ctgaggcttc gttttttgaa gccgtgagga tctcatcctt
     4081 ttc
//
LOCUS       DAOSOL010000111         4049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_22279_length_4049_cov_4.146970, whole genome shotgun sequence.
ACCESSION   DAOSOL010000111 DAOSOL010000000
VERSION     DAOSOL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22279_length_4049_cov_4.146970"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /gene="cbiT"
                     /locus_tag="PK171_03715"
     CDS             <1..686
                     /gene="cbiT"
                     /locus_tag="PK171_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HOT05331.1"
                     /translation="NLSLPGEFIQSGTVQELCSTHLSQSDLALIYLSPGERKGIGFFE
                     DEDYIRTNIPFSKKETRMMVCSLLELLPAMTVLEIGGGSGGITIDIARRISEGILFSL
                     EKNQEACQLIQQNCERFRIDNVQCIHSTAPNDIPQKLFDRIFIGGSGGSLSSIIDNSS
                     KVLKKGGILVMTALSLLHVTEGINRIKNNPFQSLSIFQQSISRIDPNDKNLMMKSLNP
                     IFFIKAVKS"
     gene            683..1444
                     /gene="cobM"
                     /locus_tag="PK171_03720"
     CDS             683..1444
                     /gene="cobM"
                     /locus_tag="PK171_03720"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HOT05332.1"
                     /translation="MTPVYFVGAGPGDPELITLKGKKLLEKADCVIYAGSLINHGILK
                     VCSPQTKLVDSSTHTLEEIIDYMVQEVAQNHLVIRLHSGDPSLYGAIQEQMNELDNRG
                     ISYQVIPGVSSVGAVASRIKKEYTLPHGTQTLIITRMGGQTPVPESEQLSTLAAHRTS
                     MVILLSIGFIDQVVEELSKIYPIETPIICAYRVTWPDERIIQSTLEKITQAVKEAGIH
                     RSATLLIGPFLNHSEHFRSYLYGDWINENRHLPFS"
     gene            1416..3116
                     /locus_tag="PK171_03725"
     CDS             1416..3116
                     /locus_tag="PK171_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HOT05333.1"
                     /translation="MKTVIFPFHKHYALLAKNIADLNFLQPVQFVEGWKNSQKNLQKK
                     WETDVVLIFIGSLGIATRLTAPFLKNKRTDPGIIVIDGAANFCIVVNGGHSRSMNTLC
                     RLIAEQLQLIPVITTGTEACGYLSPEEIAERLDCQIEDPHYLLKTIQSRLIEGEIIPF
                     CFDESFSPPYFPGYQIQSSHHQFPQEPFLYLTDRISFCPKAVFVRPRSLVIGTGFRKQ
                     FSIEIFEEFYHDFIEKNKYSHLSVAEVLTLDRKVPSLLPLVQSKSIKITGYSLDQLKN
                     VTGDFHSPCAQKHLKIPGLSEPSLIIRGAEILVGKTVYPDMTFALGRLPWKPQGSLTF
                     IGIGPGQPNLMTIQAIQALEESDLIIGYETYLKMVPFRYQNRILSICTKMGEEIQRVK
                     KAIELVKKGNRVAVISSGDSGVFGMTAPGVELAIQENIPWRIVPGITAALWGASLIGS
                     PLVNGFCVISLSDYLIGWDSITTNLQLLAQTELPIVVYNLVKKDRSQKIEELVDIVQQ
                     NRGLETNVAIIKKDGTKEFVQLQQLIHSNLDMNTLLIIGGLRNKRLGEYLITDRGYTL
                     "
     gene            3113..3811
                     /locus_tag="PK171_03730"
     CDS             3113..3811
                     /locus_tag="PK171_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HOT05334.1"
                     /translation="MKLSVVGLGPGDPELVTVKAKKVIESSRLIFVPSMKSDLSSRAY
                     HTALPYISKSTSIVPLYFPYFSNPRFSEIIQSNVDSMMTKLKLYDQAAFLIIGDHFLY
                     SSYFQLHQFLQEQYPEIKIDIIPGLSAYQLALSHLQIPAVGKNQALSVITGEANHEMI
                     ALFLDLSDTVIIYKINQISNLAELELLTREFSVRKICCEIGTDQEMIQDLNHKTAYEH
                     YGYFSSIILKKTKQ"
     regulatory      3909..4036
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         1219 a          849 c          793 g         1188 t
ORIGIN      
        1 tcaacctgtc tcttccgggg gaattcatcc aatcgggtac tgttcaagaa ctctgcagta
       61 ctcacctttc ccaaagcgat cttgccctca tttacctttc cccgggagag agaaaaggga
      121 tcggtttttt tgaggacgag gattatattc gcaccaatat ccctttttca aaaaaagaga
      181 cccgaatgat ggtttgttca ttactggagc tcttaccagc catgactgta ctggaaatag
      241 gtggcggttc aggtggcatt accattgata ttgctcgacg gatatccgaa ggtattttat
      301 tttctctgga aaaaaatcaa gaagcctgtc agctcatcca gcaaaactgt gagcgtttta
      361 ggatcgataa tgttcaatgc atacatagta cagcaccgaa cgacattccc caaaagctct
      421 tcgatcgcat ttttattgga ggaagtggag gttctctttc ctcgattatc gataatagtt
      481 ctaaagtatt aaaaaaagga ggaatcttgg ttatgactgc tctatctctt ttgcatgtga
      541 ctgaggggat aaatcggata aaaaataatc cttttcagtc tttatcaatt tttcagcaaa
      601 gtatatcacg gattgatccc aatgataaaa acttgatgat gaaatcgctc aatcccattt
      661 tttttatcaa ggcagttaaa tcatgacccc ggtctatttt gtcggagcag gaccaggaga
      721 tcctgaactc ataaccctaa aagggaaaaa gcttttagaa aaagccgatt gtgttatcta
      781 tgccggttct ttgataaacc atggcattct aaaagtatgt tctcctcaga cgaaattagt
      841 tgacagctca acccatacgc tcgaagaaat catcgattat atggtccaag aagtcgctca
      901 aaatcacctt gttattcgac ttcattcggg agacccttcc ctttatggag caatccagga
      961 acaaatgaat gaattagata accgcggcat cagctatcaa gtcatacccg gtgtgtcttc
     1021 ggtcggtgca gtagcttcca ggattaaaaa agaatacact cttccccatg ggactcaaac
     1081 tctgatcatt actcgaatgg gcggacaaac tcctgtacca gaaagcgaac agctttcaac
     1141 cctggcagct catcgaacct cgatggtcat cctcctcagc attggattta tcgatcaggt
     1201 ggttgaagaa ttatcaaaaa tatatccaat agagaccccc ataatctgtg cttatcgagt
     1261 aacctggccg gatgaacgta ttattcagag caccttggaa aaaattactc aagcagtgaa
     1321 agaggctggg attcaccgtt ctgcaacgct tctgattgga ccttttttaa atcattcgga
     1381 gcactttcga tcatatcttt atggagattg gataaatgaa aaccgtcatc ttcccttttc
     1441 ataaacacta tgccctcttg gcaaaaaata ttgccgattt gaattttttg caaccagtgc
     1501 aattcgtcga aggttggaaa aactcccaaa aaaaccttca gaaaaaatgg gaaactgatg
     1561 tcgtcttaat ttttatagga agccttggaa ttgctactcg attgaccgcg ccctttctca
     1621 aaaacaaacg gaccgatccg ggaatcatcg tcattgatgg agctgccaat ttctgtatcg
     1681 tggtgaacgg aggccattcc cgttccatga ataccctctg ccgtttaatt gctgagcagc
     1741 ttcaactcat accggtgatt acgactggaa ccgaggcctg tggttatctc agtccagaag
     1801 agatcgccga acgattggat tgccaaattg aagatcctca ttatcttcta aaaaccattc
     1861 aaagtcgtct gatagagggc gaaattattc ccttttgttt tgatgaatcc ttttcgccac
     1921 cttatttccc cggttaccaa attcaatcat ctcatcacca atttccccaa gaaccctttt
     1981 tatatctaac cgaccggatt tctttttgcc caaaagcggt ttttgtgcga cctcgctctt
     2041 tagtaatcgg aactggattt cgaaaacagt tttccatcga aatatttgaa gagttttacc
     2101 atgattttat tgaaaaaaat aaatattccc acctttcggt agctgaggtc ctcactcttg
     2161 accgaaaagt tccttcgctt ctccccttgg ttcaaagtaa gtcaatcaaa attaccggat
     2221 attcactaga tcaattaaag aatgttaccg gggattttca ttctccttgt gcccaaaaac
     2281 atttaaaaat tcccggttta tctgaaccgt cgttgattat tcgtggtgct gagattttag
     2341 tgggaaaaac cgtctatccc gatatgacct ttgctttggg acgactccct tggaaacctc
     2401 agggaagttt aacgtttatt ggaatcggac ccggtcagcc caacctgatg acaatccaag
     2461 ccatacaagc tcttgaagaa agtgacttga tcattgggta tgaaacctat ttaaaaatgg
     2521 tgccatttcg ttatcaaaac cgcatcctat cgatttgtac caagatgggg gaagaaatcc
     2581 agcgggtaaa aaaagcaatt gaattggtta aaaaaggaaa tcgggtagcg gtaatcagca
     2641 gtggcgattc aggggtgttt ggaatgacag ctccgggggt ggaactggca atccaagaaa
     2701 acattccatg gcgcatcgtt cccggaatta ctgcagcctt gtggggagct tctctgattg
     2761 gatcaccgtt ggttaatggt ttttgtgtta tcagtctgag tgattactta attggctggg
     2821 atagcattac caccaatctt caactccttg ctcaaactga gcttccaatc gtagtctata
     2881 acttagttaa aaaggatcgt tctcaaaaaa ttgaagagct ggttgatatc gttcaacaaa
     2941 atcgcggatt agaaaccaac gtggccatca ttaaaaaaga tgggaccaag gaattcgtcc
     3001 agctccaaca actaattcat tccaatcttg acatgaacac gctgttgatt attggagggc
     3061 taagaaacaa acgccttgga gaatatctca ttactgatag ggggtatact ctttgaaact
     3121 gtctgtggtt ggtctcggtc ctggagatcc tgaactggtt accgtgaaag caaagaaggt
     3181 tattgaaagc agccgattga tttttgtccc ttcgatgaaa agtgatttat ccagtcgagc
     3241 ttatcatact gctcttccct acatctcaaa aagtacctca attgttccgt tgtattttcc
     3301 ttatttctcc aatcctcgat tttcagaaat tatccaatcg aatgttgatt cgatgatgac
     3361 caagttaaaa ctttacgatc aagcggcttt tctcattatt ggagatcatt ttttatattc
     3421 cagttatttt cagctccatc aatttttgca agaacaatat cctgaaatta aaattgatat
     3481 tattcctggt ttaagtgctt atcaattagc tctatctcac cttcagattc cggcagtggg
     3541 aaaaaaccaa gccttatcag ttattaccgg tgaggcaaac catgaaatga tagctttgtt
     3601 tttagattta agcgatactg tgattattta taaaatcaat caaatttcaa atttagctga
     3661 attagaattg ctaaccagag agttttcagt tcgaaagatc tgttgcgaaa taggaaccga
     3721 tcaagaaatg attcaggatt taaatcataa aaccgcatac gaacactatg gatatttctc
     3781 aagtatcatt ttaaagaaaa ccaaacaata aaattttttc aataatagat catttttcct
     3841 tgcaaaatta aattatttaa actaagattc ataccagtag acaattgaat agcattcttc
     3901 cacgggagac tgggaaaccg gtgaaaatcc ggtgcggtcc cgccactgta agaaggaacg
     3961 aaaggttcaa aaaaccactg gcgaataaag ctgggaagga gaaccgagta ggatgcgcct
     4021 tcaagtcagg ataccaaccc gaggaatca
//
LOCUS       DAOSOL010000112         3990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_22738_length_3990_cov_3.663278, whole genome shotgun sequence.
ACCESSION   DAOSOL010000112 DAOSOL010000000
VERSION     DAOSOL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22738_length_3990_cov_3.663278"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /gene="thiD"
                     /locus_tag="PK171_03735"
     CDS             complement(<1..599)
                     /gene="thiD"
                     /locus_tag="PK171_03735"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOT05335.1"
                     /translation="MLKRVLTIAGSDSGGGAGIQADLKTFCAFGVYGMTAITAVTAQN
                     THEVLSITALNSAAVYNQIKAIAEDIGVDAVKTGMLFNAEIIEAVVEAIKNFSLPQLV
                     IDPVMVSKSGRYLLESEAIHSFKEKILPHAQVVTPNLNEASVLSGISVLTPDEMKQAA
                     RIIHQMGPKYVLIKGGHLAGEEMMDILFDRKKDVIFPVQR"
     gene            complement(692..1702)
                     /gene="thiL"
                     /locus_tag="PK171_03740"
     CDS             complement(692..1702)
                     /gene="thiL"
                     /locus_tag="PK171_03740"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOT05336.1"
                     /translation="MSTLTDIGEFGLIDLIKKKFTPYGPETVVGIDDDCAVVTGREGF
                     YTLYTCDSQVEGSHFITDLVPPYLLGQKVLSVNLSDIAAMGGTPRFALLSLVLRSNVS
                     IRWIDTFLEGFRYKANQYAVEVIGGNLAHTEGKLIFDVTCIGEVKRNRLLLRNQARVG
                     DLILVTGSLGDAAAGLKILLEEQKMNTAKKQYLLNRYFLPEPRVEVGRILADSKDRIA
                     LIDISDGFAQDLGHILKQSQVGAIIDPERIPLSRQLITWCSEQNCYPLDYAFSGGEDY
                     ELIFTTTPQNADELRNKIQREVGVPVTIVGEITTGSNLIFEKNEQNYEFHTQGWNHFS
                     SF"
     gene            complement(1690..2334)
                     /gene="thiE"
                     /locus_tag="PK171_03745"
     CDS             complement(1690..2334)
                     /gene="thiE"
                     /locus_tag="PK171_03745"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOT05337.1"
                     /translation="MIDYSLYVITDRDLPRNRTLIEVIEEVIQGGATIIQLREKNLPT
                     RIFFENARTVRKITKTTGIPLIINDRLDIALAVGADGVHLGDEDLPLRYAREIAPHFI
                     IGYSADSVHQAQKAEEDGANYLGVGSVFPTQTKIDAGMAIGTKRLREIKTAVSIPVIA
                     IGGITLENLPEVIQSGVDGVAVVSAIVAADSPFETTKRFRYIIDTFRKGKTCQP"
     gene            complement(2331..3143)
                     /gene="thiM"
                     /locus_tag="PK171_03750"
     CDS             complement(2331..3143)
                     /gene="thiM"
                     /locus_tag="PK171_03750"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOT05338.1"
                     /translation="MDKKIIQQCVSLLPIIRQKKPLIHHITNMVTANDNANVTLAIGA
                     SPVMALSKVEVEEMAAHADSLVLNLGILNPDVIESCRLAGKVANQKRIPVILDPVGAG
                     ATQYRTNSAKEILSEIKIAIIRGNAGEISALLGEPRLIQGVDAVSTNLSISDLAREAS
                     RRYGTIVAVTGKVDFISNGIHLISVFNGHPWLTFLTGTGCMVSSLCATFASVEEDHLI
                     STTAAIGFFGVCAEIAAKKVEGPGLFHQVLFDVIYNMDVENFASSLQVEVET"
     gene            complement(3128..>3990)
                     /locus_tag="PK171_03755"
     CDS             complement(3128..>3990)
                     /locus_tag="PK171_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOT05339.1"
                     /translation="NNRFLWILIAGLGSTVWAMIGHRWWKWIQRIAVVALAALCVVMT
                     YVVMANLPLSFLKNMVADGSLPWATGFDIVIAMPISWLPLVADYSRFAKSTKSAYRGT
                     WWGYFLVSSWMFLLGFFLSLATGQSDPIPGMVAMGFGVIAFMVVLFSTITTTFLDIYS
                     TAVSFMNIKPRVKEKVATFIFGISGTGLALFFPVDRYESFLYFIGSIFIPLFGVVLFD
                     YFVYRKRNVDVNQLLHQKEYDLLAWLAWAGGFVIYRLFLNYLPVFSASLPSLFSSGTL
                     YLVLKKWIKK"
BASE COUNT         1264 a          895 c          714 g         1117 t
ORIGIN      
        1 cgctgtaccg gaaaaattac atctttcttt ctatcaaaaa gaatatccat catttcctcg
       61 ccggctaaat gcccaccctt gattaagaca tattttggtc ccatttgatg gataatacgg
      121 gctgcttgtt tcatttcgtc tggggtaaga actgatatac ctgataaaac cgaggcttca
      181 tttaaattgg gtgtaaccac ctgggcatgt ggtaatattt tttctttaaa gctatggatc
      241 gcctctgatt caagcaaata acgtcctgat tttgaaacca taactgggtc aataactaat
      301 tgaggtagtg aaaaattttt tatcgcttca accactgcct caatgatctc agcattaaaa
      361 agcatcccgg ttttgactgc atcaacccca atatcttcag caattgcttt aatctgatta
      421 taaactgctg ccgaattcag ggcagtaatt gataagacct catgggtatt ctgggcagtg
      481 accgcggtta tcgccgtcat tccataaaca ccaaaggcac aaaaagtctt taaatcagcc
      541 tggatacccg ctcctcctcc tgagtcagaa ccggcaatag taaggactcg ttttaacatg
      601 aaaattcctc cgcactgtca ttttttcctg tttttagctt ttttttcatc agcaaatagt
      661 cgttagacat tgctgttaga atgaatattt tttaaaaaga ggaaaaatga ttccatccct
      721 gggtatgaaa ttcataattt tgctcattct tttcaaatat gagattactt ccagtggtga
      781 tttcaccaac tatagtcacc ggaactccaa cctcacgctg gattttattt cgaagttcat
      841 cagcattttg aggagttgta gtaaaaataa gttcgtaatc ctctcctcca ctaaatgcat
      901 aatcaagagg ataacaattc tgttctgaac accaggtaat gagctgtcga gaaagtggaa
      961 ttctttctgg atcaataatt gcacctacct gactctgttt taaaatatgc cctaaatcct
     1021 gcgcgaaacc atcagatata tcaatgagtg caattctatc tttactatct gccaagattc
     1081 tccccacctc tactcgtggt tcgggcaaaa aatatcgatt taaaagatat tgcttctttg
     1141 ccgtattcat tttttgttct tctaaaagga tcttcagtcc ggcagcagca tcacctaaag
     1201 aaccagttac cagaatcaaa tctccaacac gggcttgatt tcgtagcaaa agtcggtttc
     1261 tcttcacttc tccaatacaa gttacatcga atatcagttt gccctcggta tgagctaggt
     1321 ttcctccaat aacctcaaca gcatactggt ttgccttata acgaaatcct tctaaaaagg
     1381 tgtctatcca tcgaatcgaa acattcgagc gaaggaccaa ggataaaaga gcaaaacgag
     1441 gtgttcctcc catggcggca atatcgctta agtttactga taaaaccttt tgtcccagta
     1501 aatagggagg aactaaatcg gttataaagt gagatccttc tacctgagag tcacaggtgt
     1561 aaagagtata aaacccttct cttcccgtaa caacagcaca gtcgtcatca atcccaacta
     1621 cggtttcagg accgtaaggt gtaaactttt ttttaattaa atcgataagc ccaaattccc
     1681 caatatcagt tagggttgac atgttttccc ctttctaaat gtatcaatta tgtatcgaaa
     1741 tcttttcgtt gtttcaaaag gcgagtcagc agcaactatc gctgagacga ctgccactcc
     1801 atcaactccg gattggatta cttcgggaag attttctaag gtaataccgc caatagcaat
     1861 gaccggtatc gaaaccgccg ttttaatctc ccttaaccgt ttggtaccaa tagccatgcc
     1921 ggcatcgatc tttgtttgag taggaaaaac acttccgacc cccagataat tagctccatc
     1981 ctcttcagct ttttgggctt gatggactga atcagccgaa tagccaataa taaaatgagg
     2041 agcaatttcc cgagcatatc ttaaaggcaa atcctcatca ccgagatgta caccatcggc
     2101 gcccactgct aaagcaatat ccagacgatc attaatgatc aatggaattc cagttgtttt
     2161 ggtaattttt cgaaccgttc gtgcattctc aaaaaagatc cgggttggaa ggtttttttc
     2221 gcgtaattga atgattgtgg cgcccccctg gataacttcc tcaatcactt cgattagggt
     2281 tctatttctc ggtaaatctc gatcagtaat cacatataaa gaataatcaa tcatgtttcc
     2341 acctcaactt gcaaagaaga agcaaaattt tcaacatcca tattatatat aacatcaaat
     2401 aaaacctggt gaaaaagccc tggaccctct acttttttag cagcaatttc cgcacaaaca
     2461 ccgaaaaatc ctatagcggc agtggttgat atgagatgat cctcttcaac ggaagcaaaa
     2521 gtggcacaga gagaagaaac catgcatccg gttccagtta aaaatgttaa ccatggatgg
     2581 ccgttaaaaa ctgaaataag atgaatccca ttactgataa aatcaacttt tcccgtcact
     2641 gccactattg ttccataacg ccggctggct tcccgagcta aatctgaaat agataaattg
     2701 gtggagacag catctacacc ttgaattaat cgcggttcac ctaaaagagc ggatatttca
     2761 ccagcattcc cgcgtataat agctatcttg atttcagaga gaatttcctt agctgagttg
     2821 gttcgatatt gagtggctcc cgccccaacc ggatctaaaa taaccggaat cctcttttga
     2881 ttggctactt tcccagctaa tcggcaactc tcgataacat ctggatttaa aattcccaaa
     2941 ttcaaaacca aagaatcggc atgagcagcc atttcttcaa cttctacctt cgagagtgcc
     3001 attactggtg aagcaccaat cgccaatgtg acgttggcat tatcattggc ggttaccata
     3061 ttggttatat ggtgaataag gggcttcttt tgacgaataa ttggtagcaa tgaaacacat
     3121 tgttgaatta tttttttatc cattttttta acaccaaata tagagtgccg gaagaaaaaa
     3181 gactgggaag ggatgcgcta aaaaccggca aataattcaa aaataatcga taaataacga
     3241 acccgcccgc ccaagccaac caagctaaaa ggtcgtattc tttctggtgc aaaagttgat
     3301 tgacatcaac atttcttttc cgataaacaa agtagtcaaa aagaaccact ccaaacaaag
     3361 gaatgaaaat tgatccgatg aaatataaaa aactttcata acggtccaca gggaagaaaa
     3421 gcgccagtcc ggtgccagat attccaaaaa tgaaagttgc aaccttttct tttactctcg
     3481 gcttaatatt cataaaagaa actgctgttg agtaaatatc taaaaaagtt gtcgtaatag
     3541 ttgaaaaaag tactaccata aatgctatga ccccaaaacc catggccacc ataccaggga
     3601 ttggatcact ttgtccagtt gccaatgaaa gaaaaaaacc caatagaaac atccaagaac
     3661 tcaccaaaaa gtaaccccac caggttcccc ggtaggcact tttggtgctt ttagcaaaac
     3721 gagaataatc agcaaccagt ggaagccagg aaataggcat agctattaca atatcaaatc
     3781 cggtagccca aggaagggat ccatcggcga ccatattctt taaaaaagaa aggggaaggt
     3841 tagccatgac tacataggtc atgaccacac aaagagctgc caatgcaacg acggcaatcc
     3901 gttgaatcca tttccaccaa cgatggccta tcatcgccca aactgtgctc cccagtccgg
     3961 ctataagaat ccataaaaat cgattattat
//
LOCUS       DAOSOL010000113         3987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_22763_length_3987_cov_4.008647, whole genome shotgun sequence.
ACCESSION   DAOSOL010000113 DAOSOL010000000
VERSION     DAOSOL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22763_length_3987_cov_4.008647"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1144
                     /locus_tag="PK171_03760"
     CDS             164..1144
                     /locus_tag="PK171_03760"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HOT05340.1"
                     /translation="MNLKRFSWLLVFLLLFLISSFALPWKVESPEQISLQVLGEKKTV
                     PIEIKNFWGFSPWIQRFQVKMVDSDLINVDQVSDQVQLSPKLLEGKTELMIRSFPVIK
                     YLTVEVNPYLEDLDKDGFPDVAELKIESDRQLFRDLFVNIARSQIAQESELWKEKDCS
                     GLVRFAYREAMKKHDKAWFQGFQGELEGLFDIQSFNYPRVPLLGTNLFRIKPGPFCYE
                     TIDNDFSVFASAQYLLSHNVVFLGRDIQVAERGDLIFFYQPGFFNFPYHVMIYEGKGK
                     VIYHTGAIEDQEGYIQEIFLDDLKKHPDRRWWPVIDNPFFLGFYRFKILE"
     gene            1202..>3987
                     /locus_tag="PK171_03765"
     CDS             1202..>3987
                     /locus_tag="PK171_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOT05341.1"
                     /translation="MKLLSWISLVFVIVFIFLSGSSFASPYISLSVPAHIYPGESIEA
                     RVNVSEIKSLQMSLFRIDEPENYILESNKEYFFSIDTWRPKRPLNEQEKWQNLKFSFF
                     QSLRGIASKVLSPSLKQSLKDILGIEGSIPARPIEITSDDYHRNSSLLKSWLQEIRYS
                     KDDFYAYQTITLNPLQEGVYLLTAAGGGKEARVIFSVTRLSLISKVDQNQMVVFVQDR
                     KSGQILEKVRMRLLENNQVVSEGETNSEGLWSKTELKQKEMTIMAQMDNEYAFMNIYP
                     YYFDSSSKLDVFIYTERPLYKPGQRVYFKSLLREKLATSYFAPPPGEKVKAVLTDYRD
                     NIYSSTELVSDEYGNLSSWFDLPMSLDEGDYLIKLSWKNSDFYRMVSIENYEKPSFEA
                     EVKTEKETYANREKVTVKVQANYYSGQPLTPGTFRYQILRYPMYYWRYGFQSEMVEEN
                     TGTLDENGEAEITFIPEVEGNAYWYEAQVGVFDAAYRGVQKTAQVKVLMGDFALVLKS
                     NRYFVGMNQNLQYTIEAFDAEEKKVDVGKVYATIYLLTWDKEKKDWNETSAKLFSFEV
                     VNGKADIDFAMEKPGYYRLECETRDEKGNTITASDTFYVTGSGFYLPTTGIELHLDKK
                     EYKVGETAIVLVVPPVTEEFPHLLTIEGEKIHQIEVKSTVKGPYQVSIPITKDYILGC
                     YVTLSAFYNGNYYNESIQIPVIPEKELLVKISTEKEVYLPQEKGKLKVTILGSDSLPR
                     KCDFSLAIVDQAIFDISWQWWEKIYEHYYQNLYNRVYTQSSWYEYFYGQGETVQNVRP
                     QFKGNGRGGAGEPTVRKYFPDTLLWMPSIKTNQQGEAEIEFTCPDNLASWRVEVRANT
                     ADEFGESVINFRTSSPFAFRVELPPFLSQGDTIQTQMTLWNEYPKQKVRLEAQTGSEI
                     KIGTYPPEVEIDAN"
BASE COUNT         1237 a          703 c          827 g         1220 t
ORIGIN      
        1 cctgtgaagc agcttttttg gatcaggttg atataccaac cgtcattttt ggaccaggac
       61 gaattgagga agctcatact gccaatgagt ttgtttcaat tacccaaatt gttgctgcct
      121 cacgaatcta ttcccttctt gcgttacggt ttttgggtgg agaatgaatc taaaacgctt
      181 ttcatggctt ttggtttttt tgcttctttt tttaatcagt tccttcgccc ttccttggaa
      241 agttgagagt ccagaacaaa taagtctcca agtattaggc gaaaaaaaga ctgttcctat
      301 cgaaattaaa aatttttggg gtttctctcc ctggatacaa aggtttcagg ttaaaatggt
      361 tgatagtgat ttgataaatg ttgaccaggt atccgatcaa gtgcagcttt ctccgaagct
      421 gcttgaggga aaaactgagt taatgatccg gtcctttccg gtgatcaagt atcttaccgt
      481 tgaggtcaac ccctatttag aagatttaga taaagacggc tttcccgatg tagcagaatt
      541 aaaaattgaa tccgaccgtc agctttttcg tgatttgttt gtcaatattg ctcgttctca
      601 aatagcccaa gaaagtgaat tatggaaaga aaaagattgt tcgggcttag ttcgttttgc
      661 ttatcgagaa gcaatgaaaa aacatgataa ggcttggttt cagggtttcc aaggagaatt
      721 ggagggcctt tttgatattc aatctttcaa ttaccctcga gttcctttat tgggaacgaa
      781 tctttttcgc atcaaaccag gtcctttttg ctacgaaacc atcgataacg acttttcagt
      841 ttttgcttcg gcacaatacc ttttatccca taatgtggtt tttttgggga gggatattca
      901 ggtggccgaa aggggagatc ttattttttt ttatcaaccg ggctttttca attttcctta
      961 tcatgttatg atttatgagg gtaaagggaa agttatttat cataccggag ccattgagga
     1021 tcaagagggt tatattcaag aaatattctt ggacgacctg aaaaaacacc cggatcgaag
     1081 atggtggccg gttattgata atccattttt tttaggattc tatcggttta aaatattaga
     1141 gtgataaata ttataaactt agttggtatg attaaaagat aaacctgaag gagggagatt
     1201 cttgaaactc ttatcctgga taagtttggt atttgtaatc gtatttattt ttttatcggg
     1261 gagttcgttt gcttctcctt atatctcgct gagcgtcccg gctcacatct atccgggtga
     1321 atcaattgaa gcaagggtca atgtgagtga gatcaaaagt cttcaaatgt cattgtttcg
     1381 tattgatgaa ccggaaaact atatcttaga gagtaataag gaatattttt tctcaatcga
     1441 tacctggaga ccgaagagac cgctgaatga gcaagaaaaa tggcaaaatt taaagtttag
     1501 ttttttccaa tcactgcgag ggattgcctc caaagtactt tctcccagtc ttaaacaaag
     1561 tttaaaggat attttaggaa ttgaaggttc aattcccgct cgcccgattg aaatcaccag
     1621 tgatgattat caccgtaatt cttcattgct gaaaagttgg ttacaagaaa tccgctattc
     1681 aaaagatgat ttttatgcct atcaaaccat taccctcaat ccacttcaag aaggcgttta
     1741 cctcctcact gctgctggtg gtggcaagga agctcgggtc attttttccg ttacccgttt
     1801 atctttaata tccaaagttg atcaaaacca gatggtagtg tttgttcaag atcgaaaaag
     1861 tggtcaaatt ttagagaagg ttcggatgag gttgttagaa aataatcaag tggtcagcga
     1921 aggagaaacc aattctgaag gtctttggtc aaaaaccgaa ctgaaacaaa aagaaatgac
     1981 cataatggct caaatggaca atgaatatgc ttttatgaac atttatcctt attattttga
     2041 tagcagctct aaacttgatg tttttattta tactgaaagg ccgctctaca agccgggtca
     2101 acgagtttat tttaagtctc tattacgaga aaaattagca accagttact ttgcgcctcc
     2161 tcctggagag aaagtgaaag cggttcttac cgattatcga gataatatct attccagtac
     2221 agaactagtt tctgatgagt atggaaatct atcatcctgg tttgaccttc cgatgtctct
     2281 tgatgaggga gattatttga ttaaactatc ctggaaaaac agcgatttct atcggatggt
     2341 atccattgaa aactatgaaa aaccatcatt tgaagctgaa gtaaaaaccg aaaaagaaac
     2401 ttacgctaat cgggaaaaag tgaccgttaa agttcaagcc aattattatt cagggcaacc
     2461 actcactcct ggaacttttc gatatcaaat ccttcgctat ccaatgtatt actggagata
     2521 tggctttcag agtgagatgg ttgaagaaaa tactggaact cttgacgaaa atggagaagc
     2581 ggaaataact tttattcctg aagtagaggg gaacgcctat tggtatgagg cgcaagtcgg
     2641 agtatttgat gctgcttatc gaggagtaca aaaaacagca caagtaaaag ttttgatggg
     2701 ggattttgct ctggttctta agtcgaatcg ctatttcgtt ggaatgaacc aaaatctcca
     2761 atatactatt gaagcttttg atgctgaaga aaaaaaggtg gacgttggaa aagtttacgc
     2821 aacaatatat ctcttgactt gggataaaga aaagaaagat tggaatgaaa catcggcaaa
     2881 gcttttttcc tttgaagttg tcaatggaaa ggccgatatt gattttgcta tggagaaacc
     2941 cggatactac cgtttggaat gcgaaacccg tgatgagaaa gggaatacca taaccgcttc
     3001 tgatactttt tatgtcaccg gatctgggtt ttatcttcct actaccggaa tagaacttca
     3061 tctcgacaaa aaagaatata aagtcggtga aactgcaatt gtacttgttg ttccaccggt
     3121 taccgaagaa tttcctcatt tattaaccat tgagggagaa aaaattcacc agattgaagt
     3181 gaaatctacg gttaaaggac cctatcaggt ttcgataccg ataaccaaag actatatatt
     3241 gggttgttat gtaactttgt cggcctttta taatggtaat tattacaatg aaagtattca
     3301 aattccagta attccagaga aagagctcct ggtaaaaatt tcaaccgaaa aagaagttta
     3361 ccttcctcaa gaaaaaggga aactcaaagt aacaatttta gggagcgatt cccttcccag
     3421 aaaatgcgac ttttcattag ccattgtgga tcaagcaatt tttgatatat cctggcaatg
     3481 gtgggaaaag atctatgagc attattatca aaatttatat aatagagttt atactcagtc
     3541 atcatggtac gaatattttt atggtcaggg agaaacagtt caaaatgttc gtccacaatt
     3601 taagggaaat ggtcgaggcg gagcggggga accaaccgtg cgaaaatact tccccgacac
     3661 tctgctttgg atgccaagta taaaaaccaa tcaacaagga gaagctgaaa ttgaatttac
     3721 ctgccctgac aatctagcca gttggagagt tgaagtcaga gccaacaccg cggatgaatt
     3781 tggagaatca gttattaact ttagaacatc cagtcctttt gctttccgag tagaattgcc
     3841 tccatttctt tcacagggag atacgatcca aacccaaatg actctttgga acgaatatcc
     3901 aaagcaaaag gttcggcttg aagctcaaac tggttctgaa ataaaaattg gaacctatcc
     3961 ccctgaggtg gagattgatg ccaatca
//
LOCUS       DAOSOL010000114         3984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_22782_length_3984_cov_4.277424, whole genome shotgun sequence.
ACCESSION   DAOSOL010000114 DAOSOL010000000
VERSION     DAOSOL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22782_length_3984_cov_4.277424"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PK171_03770"
     CDS             complement(<1..925)
                     /locus_tag="PK171_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOT05342.1"
                     /translation="MASNGSIILDQSGKLNDWIEIHNTSSQPIDISGYFISDKLDDPL
                     KWQIPEGYHQETIIPDGGFIVLIADGRPDHGPLHLDFELSKTGESIILTEPDGDTTID
                     VVTFGPQWKDVSFGRSTTSPETWVFFLSPSPGNPNQGAKTASYWIAGLYLFYKSYREL
                     FLTFFAFLIILTYISIRLFQVLKKLRQVEEKQTSLIQAVPEIILQLDHKGQVEWLNQP
                     GIGFFGENTLGKDCRELFKTAISSVSIEDIIQNQNFGSSLSDAHFITEFPSHKDEFQK
                     IIDWSSSPCLLQGSKKGILLVGRDITQQQYNE"
     gene            complement(1180..1255)
                     /locus_tag="PK171_03775"
     tRNA            complement(1180..1255)
                     /locus_tag="PK171_03775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1220..1222),aa:Arg,seq:tcg)
     gene            1428..2543
                     /locus_tag="PK171_03780"
     CDS             1428..2543
                     /locus_tag="PK171_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT05343.1"
                     /translation="MSDIYLQKLAKLLLSYSTRVQPGEKVFITAEDVAIPWIEAVATQ
                     AFQMGAVVRTIVELDSVRLAKFRYGSREAVHAEDLFFKTIVDHADVFLTAWGSWNTKS
                     RTNIDSEKIKMSIQANEAIRRKFSQRMGDGSLRWCGTQYPTHADAQEAGMSLEEYQDF
                     VYRAGYLHTENPIAEWMRIEKDQEKWIGYLNSKKELRIVSEKTDLRVGIADRTWINCC
                     GRENFPDGEIFTSPVENQIEGLITFSFPGIYMGKEVEDISLRVSNGHVVEARAKKGED
                     LLRALLTTDNGAGFFGEVAIGTNYQIQQFTRNMLFDEKIGGTIHMALGDSMLEAGGTN
                     QSSIHWDMLCDMRDQGEIYADGELFYKQGKFIEDVLG"
     gene            2647..3156
                     /locus_tag="PK171_03785"
     CDS             2647..3156
                     /locus_tag="PK171_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05344.1"
                     /translation="MKKIFSGVTVALLVVLMVSSFALARGRGENNYGRQLTGKTVEMS
                     GTIQAIDFTPPQIEIKVDADGKVIEVELGPVKQYDPKDFQIGAAISLTGEFVAENVFL
                     PYSFKVNNKTYELRDADGYPLWTGGKNDNSNRGNQNQNDNWDCRGRCSRNRDGNGRGM
                     MENRGKCNR"
     gene            3304..3822
                     /locus_tag="PK171_03790"
     CDS             3304..3822
                     /locus_tag="PK171_03790"
                     /inference="COORDINATES: protein motif:HMM:NF039358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6141 family protein"
                     /protein_id="HOT05345.1"
                     /translation="MNIGNSNQVIFKEIQSVRSQWIWGLVLIPIVVFLYGMIQQLIFK
                     KTWGNNPMSDTGLLIVGLIVIIGLPLFMFLNRLEIQVSSYGVYYRYRPFHFQYQRIPK
                     ENLVSYQIVHYQPLRDYGGWGIRYGKYGKAYTLSGDIGVQLVTIDQKKILLGTLRPED
                     MQKALDSILKKH"
BASE COUNT         1292 a          633 c          869 g         1190 t
ORIGIN      
        1 tttcattata ttgctgctga gttatatcac gccctaccaa aagaattcct tttttgctac
       61 cttggagaag acaggggctt gagctccaat caataatttt ttgaaattca tctttatggg
      121 aaggaaactc ggtaatgaaa tgggcatcag aaagagaact cccaaaattt tgattttgga
      181 tgatatcttc aatggatacc gatgaaattg cagttttgaa taattcccga caatcttttc
      241 ctaaggtatt ttcaccgaaa aagccaatac caggttggtt aagccattct acttgtcctt
      301 tgtggtcgag ttgaaggata atttcaggaa cggcttgaat caaagatgtt tgtttttctt
      361 cgacctgtct aagcttttta agaacctgaa aaagtcgaat gctgatgtag gtgagaatga
      421 ttaaaaaagc aaaaaaggta aggaaaagtt cccggtacga tttataaaag agataaagac
      481 cagcaatcca ataggaagcg gtttttgctc cctgattagg atttcctggg gaaggtgaaa
      541 gaaagaacac ccaggtttcc ggactcgttg ttgacctccc aaaggagaca tctttccact
      601 gtggaccaaa ggtgacgaca tcaatggttg tgtccccatc tggttcagtt aaaataatgc
      661 tctccccggt tttagaaagc tcaaaatcga gatgaagcgg accatgatca ggtctcccat
      721 cagcgattag aacgataaaa ccaccatcag gaatgatcgt ttcctgatga tatccttccg
      781 gaatctgcca ttttaaggga tcgtctaatt tgtcggatat aaaataacca cttatatcga
      841 taggttggga tgatgtatta tgaatttcga tccaatcatt aagttttccc gactggtcta
      901 aaattatgga accattggaa gccataaatt cagtaataag aagggaggca aagctctctg
      961 ctgaggtatt ccaaaaaagg aatagaaata ttataaatac tacaatatat gaaggacttt
     1021 tgagctcata aagatgcctt attttcattg aaatcattcc ttcaaatatt tttacctgaa
     1081 aaaataaaaa attttcaata ttttaccata cccaagattt aatgaaaaat attattcctt
     1141 tgggaagggg cactcttgaa gtgaggtgga gaaaaaaaat ggcgcgcctg gagggagttg
     1201 aacccccaac cttcggatcc gaagtccgcg gctctgtcca ttgagctaca ggcgcacccc
     1261 ttcttttaat attataaaaa aattttcgag aaaagaaaag agttaaagct tggttattcc
     1321 tgtttgtttt tgaacagaat cagatagtaa ttttcaaatt ggataaaatt ttgatatact
     1381 tattaagttt ggttttataa aaaaaaaatt tatatcgagg gatgaatatg tccgatattt
     1441 atttacaaaa attagctaag cttctccttt cttattccac ccgggtccag cccggtgaga
     1501 aagtctttat tacagcagag gatgttgcta ttccttggat tgaagcagtt gcaacccaag
     1561 cttttcagat gggagctgtg gtacgaacca ttgtcgagct tgattcggta agattggcaa
     1621 aattccggta tggaagtcgg gaggcagtcc acgctgaaga cctttttttt aaaaccatcg
     1681 tggatcatgc tgatgttttt ttgactgctt ggggaagttg gaacaccaag tctcgtacca
     1741 acattgattc cgaaaaaatc aaaatgagca tacaagccaa cgaagcgatt cgtcgaaaat
     1801 tttcccaaag aatgggagat ggaagtcttc gctggtgtgg aactcagtat cctactcatg
     1861 ccgatgccca ggaagctggt atgagcttag aagaatacca agattttgtt tacagagcgg
     1921 gatatctcca taccgagaat cccattgctg agtggatgcg aattgaaaaa gatcaggaga
     1981 aatggattgg ttatctcaat tcaaaaaaag aactgcgtat tgtcagcgaa aaaaccgatt
     2041 taagggtggg aattgccgat cgtacctgga ttaattgttg tggacgagaa aatttccctg
     2101 atggagaaat ttttacctct ccagttgaaa atcaaatcga agggcttatt acttttagtt
     2161 ttccagggat ttatatggga aaagaagttg aagatatttc tttaagagtg agtaacggtc
     2221 atgtcgtcga agcccgggca aaaaaaggtg aggatttact ccgtgctctt ctcaccactg
     2281 ataatggagc tggttttttt ggagaagtgg ctattggaac gaattatcaa atccagcaat
     2341 ttacccggaa tatgttgttt gatgaaaaaa ttggaggtac gatacacatg gctttgggag
     2401 actcgatgct ggaagcaggt gggaccaatc aatcttcaat tcattgggat atgctttgcg
     2461 atatgcgtga ccaaggggag atctatgctg acggagagtt gttttataaa caaggcaaat
     2521 ttatagaaga tgttttaggg tgaagggtta aattctaatc aaactttaag ggaattctaa
     2581 ggttattcta aggttgggag gctaaaatag gaacaatcag taaaaagtcg aaaggagctg
     2641 gaaataatga agaagatttt tagtggtgtt accgtagctc ttctggttgt tcttatggtt
     2701 agtagttttg ctttggctcg tggacgagga gaaaataatt atgggagaca gctgactggt
     2761 aaaacagttg aaatgagcgg aacaatacaa gccattgatt ttactccccc acagattgaa
     2821 attaaagttg atgcggatgg caaagtgatt gaggttgagt tgggtccggt aaaacaatac
     2881 gatccaaagg atttccaaat tggagctgct atttctctga ccggtgaatt tgttgcagaa
     2941 aatgtatttc ttccctattc ctttaaggta aacaataaga cctatgaatt aagagatgct
     3001 gatgggtatc ccctctggac aggcggaaaa aatgataata gtaatcgagg aaaccaaaac
     3061 caaaatgaca attgggattg tcggggacga tgcagtcgta atcgagatgg aaacggtcga
     3121 ggcatgatgg aaaaccgggg aaaatgtaat cggtaaaaat ggtttatata aaggctgctc
     3181 attacatggg cagccttttt ttttgaattt tccttctata attatattca gagtaatttg
     3241 cattaagctg aaaaatcaat taaattattt taaagaaaaa gatgaatata ttgagggcat
     3301 aagatgaata ttggaaattc taatcaagta atttttaaag aaattcaatc cgttagatct
     3361 caatggatct gggggttagt tttgattcct atagtggttt ttttgtatgg gatgatacaa
     3421 cagttaattt ttaaaaaaac ttggggaaat aatccaatgt cagatacagg attattaatc
     3481 gttggattga ttgtcattat cggtctacca ctgtttatgt ttttaaatcg tttagaaatt
     3541 caagtgagta gttatggggt ttattatcgt tatagacctt ttcatttcca atatcagaga
     3601 attccaaaag aaaacctggt gagttatcaa attgtccatt accaaccttt gcgagattat
     3661 ggaggatggg gaatccgtta tggaaaatac ggaaaagctt atactctctc tggagatatc
     3721 ggagttcaat tggtcactat tgatcaaaag aagattttac tcggcactct tagaccggaa
     3781 gacatgcaaa aagctcttga ttcaatactc aaaaaacact aactaatcta ctcgcttgct
     3841 tcactgaccc atcgaatatt ttattgttat ttattataag taaacagtct gatagttcaa
     3901 aaagaaagga attgggttct tggtgtcaaa ggaggtgaat gataacggat gatttagaaa
     3961 ataaagagaa agctgaaaaa atcc
//
LOCUS       DAOSOL010000115         3964 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_22922_length_3964_cov_4.836019, whole genome shotgun sequence.
ACCESSION   DAOSOL010000115 DAOSOL010000000
VERSION     DAOSOL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22922_length_3964_cov_4.836019"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            452..817
                     /locus_tag="PK171_03795"
     CDS             452..817
                     /locus_tag="PK171_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOT05346.1"
                     /translation="MSSRDIEKIEKIQSDRKAANEFYSQNSEVYRSFVSMERKTYSNG
                     ELEKRYKEMIAIGISIIINCESCLEWHIKEALRSGASEKQIIEAIEVGIEMGGGPATV
                     SARFAMKVLEYYRQNTLMA"
     gene            complement(876..1967)
                     /locus_tag="PK171_03800"
     CDS             complement(876..1967)
                     /locus_tag="PK171_03800"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT05347.1"
                     /translation="MNIPKTMKAGVLYGYKNLTLQEIPVPEPKENEILLKVGACGICG
                     SDIRYYYGENAWALHTLGFNEPTPGAAILGHEVAGTITQLGTHSSEWQEGDRVGALAF
                     KSCGVCEFCLRNLPNLCAFQHHIGHDGRWKEVDYAPGGYSEYMPIWKDKIFSLPEHVS
                     FIEATQLDGLAVAIHAINRSRLTIGESLVVVGSGAIGLLVAQVGITLGAGKVMVLDTR
                     EKPLNIANQLGIREARKINQDNLKKEIQDFTNQKGADVIIDTVGTGKTITKSLRSLNR
                     FGRLVLVATTEDEITIKPTDLAGERIITTSANNLYRNYPQAISLLASGKVQTKPFITH
                     VFPLESLGEAFQLALDKENNDVIKIVLVP"
     gene            complement(2042..2851)
                     /locus_tag="PK171_03805"
     CDS             complement(2042..2851)
                     /locus_tag="PK171_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05348.1"
                     /translation="MKFLKSLLFYLGLFIIIIPTVFVFYWMISGGFKTQLQIISSPPV
                     FFFEPNIKNYIEVITQHNFGLYMWNSFVVAALSTLVALLVGIPAGYSISRFRMRTSVF
                     LLTVSRMVPFVSYLMPWFITFRRWGLIDSYTGLTLAHLIVNMPIVVLLMSTFFEEVPY
                     ELEEAAEIDGASKVNVFKYVSLPLARNGVITSAILSFVFSWNQFLFSLILSGPRTQTV
                     PIAVFNFMTYEEIAWGGLLAAATMITLPVLILTIFIHKYIVRGLTLGALKG"
     gene            complement(2848..3726)
                     /locus_tag="PK171_03810"
     CDS             complement(2848..3726)
                     /locus_tag="PK171_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05349.1"
                     /translation="MLKRNTTLKWILLSPAFALTGILIAYPLAYNFYLSFHSWYGSAT
                     RLPRFEGFSNYIATLSDPRFWNSIRVTIVFTILALGIEIVAGFFIALYINREFRGKNF
                     IKALFVFPFAATPVAIALVWRNMYDPTLGVINYFVSLIGRVQPVEWLTKPHLVIPSLA
                     FVDIWQWTPLVVLICMAGLEGLPREPIESAQIDGASKWQIFRRIIFPLMGPTLIAATM
                     IRSWDVIKVFDIIYVMTAGGPGFASENLNIYIYNTAFFYFNMGYASSMMVILFAIVLG
                     FNIILYQLTKRGNEKS"
BASE COUNT         1313 a          758 c          824 g         1069 t
ORIGIN      
        1 atgactcatg agattgccac gacctcaaaa aaccaggtct cgcaatgacg gattgggttg
       61 atgagatcct cacggcctta gatactgaga gggagagagc aagagggggc gagggagaga
      121 aaaaagagaa gggaaaaggg ctcaggatga cgaattaaag gcaccctccc cgccgtaaaa
      181 cggcacccct ccaaggaggg gaattgttgt attcattccc ccattgaggg gggctagggg
      241 ggtgtgcctt tctcaatttg tttttatcat tatttactca tttcggtatt ttcaggattg
      301 acagaaaaaa agcaaaggaa tatagatatt tgttcttggg aggtgatgct ttaatttttt
      361 tactcaaatt ttttagaatt taatgactta tggtttttag tatactttta tgcaatgagg
      421 taatgatgaa attattggag ggggatatac cgtgagtagc cgagatattg aaaaaattga
      481 aaagattcaa agcgatcgaa aagcagccaa tgaattttat tcacaaaatt cagaagtgta
      541 tcggtcattt gtgagcatgg aaagaaaaac ctattctaat ggagaactgg aaaaaaggta
      601 caaagagatg atcgctattg ggatttcgat catcatcaac tgcgaatcgt gcctggagtg
      661 gcatatcaaa gaagctttgc ggtctggagc atcggaaaaa cagatcattg aagccattga
      721 agtgggaatc gagatgggtg gaggaccagc aacagtttct gcccgatttg ccatgaaggt
      781 tttggagtat tatcgacaaa ataccttgat ggcttaaagc gaactcatgc aattaataaa
      841 ccgaatggcc agttctttat atactaataa gatctttatg gaacaaggac aattttaatg
      901 acatcattgt tctctttatc caaagctaat tgaaaagctt ctccaaggct ttcaagtgga
      961 aatacatggg taataaaagg cttggtttga acctttccgg atgccaagag ggaaatggct
     1021 tgtggataat tccggtagag attgttggcc gaagtggtaa ttattctttc tccagctaaa
     1081 tcggtgggtt ttatcgttat ttcatcttca gttgtggcta cgagaaccaa gcgaccaaaa
     1141 cgatttagcg agcggaggga tttagtaatt gtttttcctg ttccaactgt atcgataata
     1201 acatcagcgc ctttttggtt ggtgaaatcc tgtatttctt tttttaaatt atcttgatta
     1261 atttttctgg cctctctaat acccaactga ttggcaatgt tgagtggttt ttctcgagtg
     1321 tcaagaacca ttaccttgcc agctcccaaa gtgattccca cctgagcaac caagagacct
     1381 atggcaccgg aaccaacaac aaccaaagac tctcctatgg tcaatcggga tctatttatt
     1441 gcatgaatcg caaccgccaa accatcgagc tgggtagctt caataaatga aacatgttct
     1501 ggcaaagaga aaattttatc cttccaaatc ggcatatatt cactgtaccc tccaggtgca
     1561 taatccacct ctttccagcg accatcatgg ccgatatggt gttgaaaagc gcacaaattt
     1621 gggagattcc gtaaacaaaa ctcgcacaca ccacaagatt taaaagcgag ggcacccacc
     1681 cggtctcctt cttgccattc ggatgaatgg gtaccaagct gagtgatggt tccggcaact
     1741 tcatggccga gaatcgctgc ccccggggtt ggttcgttaa accccaaggt atgcaaagcc
     1801 caggcatttt ctccgtaata ataacggata tcacttccac aaatcccgca ggctcccact
     1861 ttcaacaaga tttcgttctc tttgggttcg ggaaccggaa tttcttgaag agttaaattt
     1921 ttataaccat agagcacacc ggctttcatg gttttaggga tattcatttt aatcctcctt
     1981 tatttgttag gtgttgataa ttttttaagg gataaaagat tttacttaaa gaaaaattag
     2041 cctaaccttt caaggcccca agagttaatc ctctaacaat atatttatga ataaaaattg
     2101 tgagaatgag gaccggtaaa gtaatcattg ttgcagcagc cagaagtcct ccccaggcaa
     2161 tttcttcata ggtcataaag ttaaaaacgg caattggtac cgtttgggtt cgagggccag
     2221 aaaggattaa ggaaaaaagg aattggttcc aggaaaacac aaaagaaaga atagccgagg
     2281 taatgactcc atttcgagcc agaggcaaag agacatattt aaagacgttg accttagaag
     2341 ctccgtctat ctcagcagcc tcctccagtt catagggaac ttcttcaaaa aaagtggaca
     2401 tcagtaaaac tacaattggc atattcacaa tcaagtgggc taaggtaaga ccggtatatg
     2461 aatctatcag tccccatcgc cgaaaagtaa taaaccaggg catcaaatag ctgacaaaag
     2521 gaaccattct tgaaacagtg agtaaaaaaa cactggttcg cattctaaat cgggaaattg
     2581 aatatccagc cgggattcca actaagagag cgaccaaggt tgatagagct gcaaccacaa
     2641 aactgttcca catatagagc ccaaaattat gttgagttat gacttctata taatttttta
     2701 tattgggttc aaagaaaaaa actggcggag aagagataat ctgaagttga gttttaaatc
     2761 cacctgaaat catccagtaa aatacaaata cagttggaat aattattata aataagccaa
     2821 gatagaacaa tagagacttt aaaaacttca tgatttttcg ttccctcgtt tggtgagttg
     2881 atagagaata atattaaaac caagtactat ggcaaagagg atgaccatca ttgaactggc
     2941 ataacccata ttaaaataga aaaaagcagt attatagatg taaatattca gattttccga
     3001 ggcaaatcct ggtcctcctg cggtcattac gtagataatg tcaaaaactt tgatgacatc
     3061 ccaggaacga atcatggttg cggctataag ggttggaccc atcaaaggaa atataattcg
     3121 acgaaatatt tgccactttg aagcaccatc aatttgagca gattcaattg gttctcttgg
     3181 taatccctcc aaaccagcca tacagatcaa aacgaccagt ggtgtccatt gccatatgtc
     3241 aacgaaagct aatgaaggaa tgactaaatg aggttttgtc agccattcaa ctggttggac
     3301 tcgacctatc agggaaacaa aataattgat gacacccaag gtgggatcat acatattccg
     3361 ccacaccaga gcaatagcta ccggcgtagc agcaaaagga aatacaaata gagctttaat
     3421 aaaattcttt ccccgaaatt ctcggttaat atacaaagca atgaaaaagc cagcaacaat
     3481 ttcaatacct aaagctaaaa tagtaaaaac gattgttaca cgaattgaat tccaaaaacg
     3541 aggatcgcta agagtggcaa tataattgct gaatccttca aaacgtggta atcgggtagc
     3601 gcttccgtac caggaatgaa aacttaaata aaagttataa gctaaggggt aagcaataag
     3661 tattcctgtt aaagcaaaag ccggggataa aagtatccat tttaaggttg tatttctttt
     3721 caacacgaat acaaagacct ccctattgaa acgccaataa aagtggatgg gatttcccat
     3781 ccacttttaa cgaataaata attggaactt ttaatcacgt gcttctatag agagtaatct
     3841 ttggatagca cttggaataa gaggttcgat atcttcacca gctatggctg atacgataat
     3901 atctccaaca acatcccgag cttcaggaac attcacgacc ggtggattcc atacggggtt
     3961 acca
//
LOCUS       DAOSOL010000116         3930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_23165_length_3930_cov_4.105032, whole genome shotgun sequence.
ACCESSION   DAOSOL010000116 DAOSOL010000000
VERSION     DAOSOL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23165_length_3930_cov_4.105032"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1018)
                     /locus_tag="PK171_03815"
     CDS             complement(17..1018)
                     /locus_tag="PK171_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05350.1"
                     /translation="MNSKERIYALLNGQAIDRIPVTPILMAFAARFIRKTYCEYYLNY
                     QVLVDSYLACQKEFNFDMVMAISDPFRETEGYGAVFSYPDNGIPVMKEPLLKTLEDRE
                     INSLPVLDPHQASRMKDRLLAVNAFHQIISKDIPILGWVEGPFAEAADLRGVQNLMMD
                     IIDQPEQVESLLKIVLETEKKFAEEQIKAGADIIGVGDAAASLLSPSLYKKYVLPFEQ
                     ELFNSIHQLGAKVKLHICGNTSGLLPYMAQTGADIIDLDFMVSLKEARHYFGSQVCIC
                     GNVDPISVMLEGTPEDVRQASLRCIEDAGMPFILSAGCEIPVATPPENLHALCQAVL"
     gene            complement(1175..2023)
                     /locus_tag="PK171_03820"
     CDS             complement(1175..2023)
                     /locus_tag="PK171_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05351.1"
                     /translation="MFKPRKKSKKQMVNTAIILIIAVFTILYLLPIYWMGITSFRPEA
                     KSMSWPPNFSPTGLVMDNYRVVFEQKSIFWYIRNSLIASITSVIISLVIGCVTAFSFA
                     FMRWKAKTKTSLLIWIISLRIMPPIAAAIPLFLIFARLRMIDTFPALILAYTFFNLSF
                     VIWLVYGFFKELPREIVEAGLIDGCSYTKLFTRVVIPLTIPGIMTVTLLTFIASWNEF
                     LFAVKLTAFNTRTIPVLISGFMIDRGLLWGQVSAVGLVSIIPVIIVALFIQRYIVSGL
                     TFGAIK"
     gene            complement(2016..2870)
                     /locus_tag="PK171_03825"
     CDS             complement(2016..2870)
                     /locus_tag="PK171_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05352.1"
                     /translation="MIEKRLKYYLILPAFIIFLSLAIYPLFFALRGSFYIWRYGMPAQ
                     FVGLKNYIDLFKSSFAWNALQNTIIYVILAVFLELIIGLVLAVLMNRELGFFRPVVRT
                     ALTIPMFVSPIVVGIIWRMIYNPHYGLFNWLLGTQGFAPTGNEYALFFVVLADVWQWT
                     PFMYVIILAALQSIPPEILEAGEIDGTTGWQKFIHITLPSISYAIIIALTLRFMDATK
                     ALDLIYTLTYGGPGGGTETIGFLIFRTAFNDLNIGSATSFALIFTVVMGILITRFLGW
                     LNRRFEIV"
     gene            complement(2952..>3930)
                     /locus_tag="PK171_03830"
     CDS             complement(2952..>3930)
                     /locus_tag="PK171_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05353.1"
                     /translation="FIKNDPEFDPSIYVEKAYQINSEWKGKQYALPFNMEGRLMFYRK
                     DIFEKEGFKVPTNREEWLNIVQSINNNPEYKEKGIWGAVYMYATEQGISYPFETYFQM
                     FDWNSIKTENGFWDENFQICIDKPKMVEAFQYWQDRLKDMPPGVESYNLPEAYQFYID
                     GKAAMTEVWPLTLYGMLLDPANADLRAKTGTADIAIGYPMSGGWGIFICADSKIQEAA
                     WEYIKFMTTAENDLFFFKEFGKGPSAKATYTDDSLKETYGDWLKGQSEAISKAVSYGK
                     IATMSELYGGNFAVITNQIMTGKLPAEEGVDKLIEELKGILERAGYPQK"
BASE COUNT         1293 a          799 c          705 g         1133 t
ORIGIN      
        1 aggaaaaaga gaatttttac aatactgctt gacatagggc atgcagattt tccggtggag
       61 ttgcaactgg aatttcacat ccagcactta gtataaaagg cataccagca tcttcaatac
      121 agcgaaggct cgcttgtcgg acatcctcag gagtaccttc taacatgacg cttattggat
      181 cgacgtttcc acatatacaa acttgagaac caaaataatg cctggcttct tttaaactta
      241 ccataaaatc caaatcaatg atatctgctc cggtttgtgc catgtaaggc aaaagtcctg
      301 aagtatttcc gcatatatgg agtttcacct tggcacctaa ctggtgaata gagttaaata
      361 gttcttgttc aaaaggtaaa acgtattttt tatagagact cggtgaaagc aacgaagcag
      421 ctgcatcacc aaccccaata atatcagctc cggctttaat ctgctcctca gcgaattttt
      481 tttctgtttc aagcacaatt tttagcaagg attctacttg ttctggttgg tcaatgatat
      541 ccatcattaa attttggact cctcgcaaat cagccgcttc ggcaaacggc ccctccaccc
      601 agccaagaat tggaatatcc tttgaaatta tttgatgaaa agcattaacg gctaataatc
      661 gatctttcat tcttgaagct tgatgaggat ccaaaaccgg aagagaattg atttctctat
      721 cctccaaggt ttttaagagg ggttctttca ttaccgggat accgttatcg gggtagctaa
      781 aaaccgctcc atacccttca gtttcacgaa acggatcaga aatggccatc accatatcga
      841 aattaaattc tttttgacag gcaaggtagc tgtcaactaa aacttgataa ttgagataat
      901 actcgcaata ggtctttcta atgaatcgag cggcaaatgc cattaatatt ggagttaccg
      961 gaattctatc tatggcttgt ccatttaaaa gtgcataaat tctttctttt gaattcattc
     1021 aaaatctccc caagtcccaa gattataaac atttattcct attattttaa aatagtcctt
     1081 tcaccacctt gaacaattaa aagtgctata aatatgataa ggagatcaaa cctagtgttt
     1141 agaattttta atttatgaga accttcttaa aaaattactt aatcgcaccg aaggtcaatc
     1201 cggaaacaat atatctctgg ataaagagag cgactatgat aaccggaata atcgaaacca
     1261 aaccaaccgc actaacctgt ccccaaagca aaccccggtc aatcataaaa ccagatatga
     1321 gaaccggaat ggttcgagta ttaaaagcag tgagcttaac agcaaacaaa aactcattcc
     1381 aggacgcaat aaaagtcaac aaagtaactg tcattatccc tgggatagta agaggtataa
     1441 ccactcgagt aaacaatttg gtatagctgc atccatcaat caaccctgct tcgacgatct
     1501 cccgtggtag ctctttaaag aacccataaa ccaaccaaat cacaaaggag agattaaaaa
     1561 aagtataggc gaggatgaga gccggaaagg tatcaatcat tcgaagcctg gcaaatatta
     1621 aaaagagagg tatggcggca gctattggtg gcatgattcg caggctgatg atccagatca
     1681 gcaaagaagt tttggttttt gccttccacc gcataaaggc aaaagaaaat gccgttacac
     1741 atccaataac cagtgaaata atcaccgacg taattgatgc gattaaactg tttctgatat
     1801 accaaaaaat cgatttttgc tcaaagacaa ctcgataatt atccatcact aaacctgtgg
     1861 gagaaaaatt gggaggccaa gacattgatt ttgcctccgg ccgaaaagag gtgataccca
     1921 tccaataaat aggtaggagg tataaaatgg taaaaacggc aataattaag ataattgcag
     1981 tgttaaccat ttgttttttt gattttttac gaggcttaaa caatttcgaa cctcctattc
     2041 aaccatccca ggaacctggt aataagaatt cccataacga cggtaaaaat taatgcaaat
     2101 gatgttgcag atccaatatt taaatcgttg aaagctgtgc ggaaaatgag gaatccaatg
     2161 gtttcggttc caccacctgg acctccataa gttaaagtgt aaataagatc taacgctttg
     2221 gtagcatcca taaaacgcaa ggtaagggca attataatag cataagaaat agagggaagg
     2281 gtaatatgaa tgaatttttg ccacccggtt gttccatcga tttcacctgc ttctaaaatt
     2341 tcgggtggta tgctttggag tgcagctaaa ataataacat acataaaggg cgtccattgc
     2401 cagacatcag ctaatacaac aaaaaagaga gcatactcat ttccggtcgg agcaaaaccc
     2461 tgagtaccca aaagccagtt gaatagacca taatggggat tgtaaatcat cctccagata
     2521 atacctacca ctattggtga cacaaacatt ggtatcgtga gtgcagttcg aacgaccgga
     2581 cggaaaaaac caagctctcg attcataaga acggctaata ctaaaccaat tatcaactct
     2641 aaaaaaacag caagaataac atatataatg gtattttgta aggcattcca agcaaaactc
     2701 gatttaaaaa gatcgatata attttttaaa cccacgaatt gagcaggcat tccatagcgc
     2761 catatataaa aacttcctct caaagcaaaa aataaagggt aaatcgctaa actcaggaaa
     2821 attataaaag caggcagtat caaataatat tttaaccttt tttcaatcat aagcagtccc
     2881 ctaatcaata gttatggaga gaaatttcaa ctattctccc cataactatt ttttttattg
     2941 gagaattagg tttatttctg aggataacca gctctttcaa gaataccttt tagttcttca
     3001 atcagtttat cgacaccttc ttcagctggc aacttgccgg tcataatctg gttggtaatt
     3061 actgcaaaat ttccgccata gagttccgac atagtagcaa tttttccata agaaaccgct
     3121 ttagatattg cttccgactg acctttgagc caatcgccgt aggtttcctt taaagaatca
     3181 tcggtataag ttgcttttgc gcttggtcct ttaccaaatt ccttgaagaa aaagaggtca
     3241 ttctcggcag tggtcatgaa tttaatatat tcccaagcag cttcctggat tttcgagtct
     3301 gcacaaatga atattcccca tccacctgac attggataac cgatggctat atcagctgta
     3361 ccggtttttg ctcttaaatc tgcattagcc ggatcaagaa gcataccata aagggtaagc
     3421 ggccagacct cggtcattgc tgcttttcca tcgatataga attggtaagc ttctgggaga
     3481 ttgtaacttt ccactccagg aggcatatct tttaaacgat cttgccagta ttggaaggct
     3541 tcgaccattt ttggtttatc gatacagatt tggaaatttt catcccaaaa accattttca
     3601 gtttttatac tattccaatc aaacatttgg aaataggtct caaaaggata agatataccc
     3661 tgttcagttg catacatgta aacggctccc cagattcctt tttctttgta ttctgggtta
     3721 ttattaatcg attgaactat gttgagccat tcctcccgat tggtcggtac tttaaatcct
     3781 tctttctcaa aaatgtcttt ccggtagaac attaaacgac cttccatgtt gaaaggaagg
     3841 gcatattgtt tccctttcca ctcggaatta atttgataag ctttttctac gtagattgac
     3901 ggatcgaatt ctggatcatt ttttataaaa
//
LOCUS       DAOSOL010000117         3867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_23636_length_3867_cov_4.814533, whole genome shotgun sequence.
ACCESSION   DAOSOL010000117 DAOSOL010000000
VERSION     DAOSOL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23636_length_3867_cov_4.814533"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK171_03835"
     CDS             <1..667
                     /locus_tag="PK171_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HOT05354.1"
                     /translation="VLVLSVFVVMAFVLGCTPPTPTPTPTPIQYPNISSIRYDSSCIK
                     AGTQLRIYGRNFGDLKGTNTVTMNGIVAAVSYWSDDEVIAVVPASSLPARELRVAVTV
                     NGVPSDEYWLYGICAAPTPTPTPTPIPLDRVDVARNIAIVKAHNDGVNVPYSVFWIKT
                     DLSTGIGSKHYQYTYYGASKWIIDITYPVIEQPDFTVNVFSEWGLTMWSGMVYYWETV
                     PYM"
     gene            complement(736..1506)
                     /locus_tag="PK171_03840"
     CDS             complement(736..1506)
                     /locus_tag="PK171_03840"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HOT05355.1"
                     /translation="MKRMLISLFTLFIFGFFPRLAIAEELMIENPSIEIDDALVVNAT
                     IEVSFGDITLTGGATKLMEGEFRYSSHEWKPLISYQVKNMVGELKVEIPANINFSLFS
                     FRDKSHKTLISLNDLVPMNLFFKVGAGKSQLNLSSMALETLTMEAGVGEVVIDLKGNT
                     SLSRLKVKTGVGEATIDLRGNWTQDIDSKIISGLGEVTIFLPQNIGVRVETKRGLGAI
                     DASNLTVNGNIYTNQQYGKTSPTLNIYLESGIGKINLY"
     gene            1695..2660
                     /locus_tag="PK171_03845"
     CDS             1695..2660
                     /locus_tag="PK171_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT05356.1"
                     /translation="MMEKKLDWEKNLTIELGVKSDPIQYRYSFDWLFNLMAEEGVYNL
                     QLGTFTEFYSLPDTYFYRLKESAQSKGIQISSIFTSHRELGGFLNPEKELAEITRKNY
                     QRMIEIAALLECPVTGSSMGSVYRDQIEYRPQGIQSFLNFIKEMMVFAKDQGLSWLTL
                     EPMSCYAEPPCNSVELKKIGDELKQFHLSQPEKTVCFGYCSDVSHGWANQEKTVIENN
                     LDYFIASFPYLYEFHFKNTDSIYNSTFGFESESLERGIIDVKQIYSILMKNQKTIPVT
                     KVIGYLEHPGPKFGRDYSDCLLERWLRESLQYLQKQWTEWSTIFS"
     gene            complement(2657..2998)
                     /locus_tag="PK171_03850"
     CDS             complement(2657..2998)
                     /locus_tag="PK171_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05357.1"
                     /translation="MIKNLTHHPVTIFKNNSVYRSIEPEGIVPRCQEERIQIGEIDEI
                     PIMTTRFGPVYNLPDKEEGVFLIVSPIVANALPERDDLLVPDLLVRDSQGHIIGAEAL
                     ARVTWWGCETF"
     gene            3146..3652
                     /locus_tag="PK171_03855"
     CDS             3146..3652
                     /locus_tag="PK171_03855"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOT05358.1"
                     /translation="MDHKKYLKIALDMGAEQAVFFRIEDIEFDARTILKCMFGCADWG
                     NANTCPSRPGSLRPWEYQKVLENYTWGIIVHSTNKYLSQKISFSIERQAFLDGYYFAF
                     SMSDCALCKECNGFKGMKCVNPKQARPSFHSVGVDVFKTVRKFGLPIETLKNEDDPQN
                     WYSAVFIE"
BASE COUNT         1214 a          698 c          732 g         1223 t
ORIGIN      
        1 tgttttagtc ctcagtgttt ttgtagtaat ggcttttgta ttagggtgta ctccgccgac
       61 tccaactcca accccaaccc ctatacaata tccaaacatt tcttctatcc gttatgattc
      121 cagctgcatt aaagccggta cacaactaag aatttacggc cggaactttg gtgaccttaa
      181 gggtaccaac acagttacaa tgaatggaat tgttgcggca gtttcctact ggtctgacga
      241 tgaagtaatt gcagtagttc ctgcatcttc attgccagcc cgagagttac gggttgctgt
      301 aacggtaaac ggtgttccca gcgatgaata ttggctttat gggatatgtg cagcaccaac
      361 accgactcct accccaactc caatacccct tgatcgggta gacgttgcaa gaaacattgc
      421 tattgtcaaa gcccataatg atggtgttaa tgttccatat agtgtatttt ggattaaaac
      481 cgacctttcg accggaattg gttccaaaca ttatcaatat acttactatg gtgcttccaa
      541 atggattatt gatattacct atccagtcat tgaacaacca gattttaccg taaatgtttt
      601 tagtgaatgg ggattaacca tgtggagtgg aatggtttat tattgggaaa cagtacctta
      661 tatgtagtaa aagtttacag cgcatacagc ttaatagctg tatgcgtttt ttatttcctt
      721 atagctatat ttgtatcagt ataagtttat tttcccaatt cctgattcaa ggtaaatgtt
      781 gagagtaggg ctggtttttc cgtattgttg gttggtataa atatttccat tgacagtcag
      841 gttagaggca tcgatagctc ctaaacctct tttcgtttca actctaactc caatattctg
      901 cgggagaaag atagttacct cacccaagcc ggatatgatt tttgaatcaa tatcctgcgt
      961 ccaatttcca cgtaaatcaa tggttgcctc tccaactcca gtttttactt tcaaacggga
     1021 gaggctggta ttccctttca aatcaatcac gacttcacca accccagcct ccatagttaa
     1081 ggtttcaaga gccatcgagg ataaattaag ttgactttta ccggctccaa ctttaaaaaa
     1141 taaattcata gggactaaat cattgagact tataagagtt ttatgacttt tatcccgaaa
     1201 gctgaacagt gaaaagttaa tgttcgccgg tatttctact ttcaattcac caaccatatt
     1261 ttttacttga taagaaatca aaggtttcca ttcatgggaa gaataccgaa attccccttc
     1321 cattaatttc gttgctcccc cagtgagagt aatatcgcca aaagatactt cgattgtggc
     1381 attaaccacc aaggcatcat caatctcaat cgaagggttt tcaatcatca attcctcggc
     1441 aattgctaag cgggggaaaa aaccaaaaat gaacagagta aatagggaaa taagcatcct
     1501 tttcatcaat aaatcaccct ccctttctct ttcattatga acttttttta ataacaattc
     1561 aagaaacctg gtatagtacc tatataaaaa agttgaaaaa gatttgacaa atttgatcaa
     1621 gggaataaaa atgaggggaa ctatttatct ccgatatttt atagaatcag aatacaaaag
     1681 aaagaggata gaatatgatg gaaaaaaagc tcgattggga aaagaacctt accatagaat
     1741 tgggagtaaa aagtgatcct attcaatatc gttattcttt tgattggctt tttaatttaa
     1801 tggcagaaga aggggtttac aatttgcaat taggaacctt tacagaattt tattcccttc
     1861 ctgataccta tttttatcga ttaaaagaat ctgctcaatc aaagggcatt cagatttcca
     1921 gtatttttac ctcccatcgg gaattggggg gttttttgaa tcccgaaaaa gaattggctg
     1981 agattaccag gaaaaattat caaagaatga tagaaattgc tgcattatta gaatgcccag
     2041 ttaccggttc ttcaatggga agcgtctatc gtgatcaaat tgaatatcgc cctcagggaa
     2101 tccaatcttt cctcaacttt ataaaagaga tgatggtgtt tgccaaggat caaggattgt
     2161 cctggttaac cctggaacca atgtcttgct acgctgaacc tccttgtaac tcggtggaac
     2221 ttaaaaaaat aggagatgaa ttgaagcaat tccatttaag ccaaccggaa aaaaccgttt
     2281 gttttggata ttgttccgat gtatcgcatg gctgggctaa tcaagagaaa accgttatcg
     2341 aaaacaatct tgattacttt atcgcctctt ttccctattt gtatgagttt cactttaaaa
     2401 ataccgattc aatatataac tcaacttttg gttttgaatc agaaagctta gaacgcggca
     2461 taattgatgt aaaacaaata tactcaattt tgatgaagaa tcagaaaact attcctgtaa
     2521 cgaaagtgat tggatatttg gaacacccag gtcccaaatt tggtagagat tactcagatt
     2581 gtttactaga acgatggctg agagaatcat tgcaatattt acaaaaacaa tggacagagt
     2641 ggagtacaat attttcttaa aaggtttcgc aaccccacca ggtaactcga gctaaggctt
     2701 cagcaccgat tatatggcct tgactatcgc gtaccaaaag atcgggaacc aataaatcat
     2761 cccgttcggg aagggcgttg gcaacaatcg gtgaaacaat gagaaatacc ccttcttctt
     2821 tgtcaggaag attataaaca ggtccaaaac gggtggtcat aatcggaatt tcatcaattt
     2881 ctccgatttg aatccgttct tcttgacagc ggggaacgat tccttcgggt tcaatagagc
     2941 gatagactga attattttta aaaattgtaa ccgggtgatg ggttaagttt tttatcatag
     3001 gactccttta tgaataaatg taaaatcatt attccatata attttagcaa ataaaaaagt
     3061 ggtatttaaa tttttcattg aaattttgat agattaggct ttagaatttt tctcattgta
     3121 atcaagaagg aagggttgtt cgaaaatgga tcataaaaag tatctcaaga tagctttaga
     3181 tatgggagcc gagcaggcag ttttttttcg cattgaagat attgaatttg atgcaaggac
     3241 tatcttaaag tgtatgtttg gttgtgctga ttggggaaac gccaatacct gtccttcgcg
     3301 tcctggatca ctgcgaccgt gggagtatca aaaagtattg gaaaactata cttggggtat
     3361 tattgttcat tccactaaca aatatctttc ccagaaaatt tctttttcga ttgagagaca
     3421 agcctttctt gatggctact actttgcttt ctcgatgagt gattgtgctc tttgtaaaga
     3481 atgcaatgga tttaaaggta tgaaatgtgt caatccgaaa caagcccgtc catcttttca
     3541 cagcgttggc gtagacgttt ttaaaacagt tagaaaattt ggtcttccga ttgaaacttt
     3601 aaaaaacgaa gatgatcctc aaaattggta ttcagcagtt tttattgaat aattaagggg
     3661 tggtcaattg aatgaaggat tgaatcataa aactcaaggt aatttttttt gttgagaaga
     3721 attgatatgg gttgggagat tcgagaggtt caaaataaac gagatttaaa atgttttgtt
     3781 aaatttcccc ttcagcttta ccgaaatcat ccctattatg ttccgccttt aatcaccgat
     3841 gaaataagta tattttctcc aattaaa
//
LOCUS       DAOSOL010000118         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_23659_length_3864_cov_4.630349, whole genome shotgun sequence.
ACCESSION   DAOSOL010000118 DAOSOL010000000
VERSION     DAOSOL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23659_length_3864_cov_4.630349"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2314
                     /locus_tag="PK171_03860"
     CDS             <1..2314
                     /locus_tag="PK171_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOT05359.1"
                     /translation="DPAWGKLDASGKQKFHHQSPLLDFRAAVENFSLSSNGKTVEILP
                     YQSSKPFRFSILERRFLDVSQPMTDSYSPLTQSPGLIVTGWWNEYSPRINGKTAVLKP
                     NERCRSLAISPDGQWVILGTEWYLRCLDRNGQELWHFRTPGACWGVNISHDGLKVVAA
                     YGDGTLHWYRLKDGRELFGAFLLPDQNHWTIWTPQGYFDASEGADELIGWHLNQGKDS
                     AAEFYPASRFFEEFYRPYLIQQIIEKTMTDQEIIEEEQIHPRVQIESIKKPPLVKILS
                     PQNQQTFDGDTAAVTFEITDQGGGIEEILIYLNGKLFNRITENLNQEKITQTVNLKLL
                     PGDNQIKIKALNQEHTESNPTEIQVHCKKEKLLPKLYILAVGVSEYDDPQVNDLFAPR
                     YDAQTFVATLVTQQGLLYREVIPTLLVNEDATRSRVVEAMNTIINQAQEEDVVIFFWG
                     GHGSSRQEGWDPPFFYALPANVFVVPRSIYQLDQDMVTSNLISDFFYRVKARKQAIIL
                     DTCHSGAAVGIYQQGLLGQEQAVRDLYRNVGVAVLAASSATESAYESVIVKAGYFTFS
                     LLYALSGDIMLDSDQKSASKLYGFTSNADYNRDGNITINEVSKFLKEKVPTLGDQTPQ
                     TFTQGLNFPLYTTNQDITPFDETLQLIFTPPNGINFLQDIQYGTRYSTPNNGELYLFD
                     YPPEVDIQGQVNQIVAGQPFQWEVAVKVGDDNAIVKAYTYSRNQKNYALLTAIYPGTG
                     VLLLIIVPSEEYQSAQSWIMPAITEVKIKK"
     gene            2404..3201
                     /locus_tag="PK171_03865"
     CDS             2404..3201
                     /locus_tag="PK171_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05360.1"
                     /translation="MKRLRFVIFIAFMITWVSGLGLAQNSGNILNLPGVGQPTPSTGQ
                     TPGQLPTLDFSQSQPATPTQQPTLPIPSTPQPGQIQILDPNQTQNQGQPIPAVWKKYN
                     LKDIVGTMDQVGRGQIEYPENWQVIPDSFNRMVSLNEDASGLVSFRLYIISYVRYSNA
                     TDYVAAIVQMLSSTVSNIRVINKDEQNITNQAVASYGASETIARYELQGNFQGTEMTM
                     CIETWVYSLYESNLGNAVIYWAPTSAYQQKYKDFFSRMMTSYKNSLK"
     gene            complement(3268..>3864)
                     /locus_tag="PK171_03870"
     CDS             complement(3268..>3864)
                     /locus_tag="PK171_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOT05361.1"
                     /translation="LGEDGPTHQPVEHISSLRTVPNLMVIRPADANETAQAWKIAVGA
                     KSPVALIFSRQKLPVIDQTKYASAEGVARGAYVLADCDSSTPDLILIASGSEVGLALK
                     AYEKMTAEGTKVRVVSMPSWELFEQQSEEYRHQVLPLSVKKRISIEAGVPIGWERYVG
                     TQGIVIGVNRFGASAPGELVMEKFGFSVENIIQKAKSL"
BASE COUNT         1154 a          866 c          755 g         1089 t
ORIGIN      
        1 cgatcctgcc tggggaaaac tcgatgcttc agggaaacaa aaatttcatc atcaatctcc
       61 gcttcttgat tttcgagcgg cggtcgagaa cttttccctc agttcaaatg gaaaaaccgt
      121 tgaaattttg ccttatcaat catctaaacc ctttcgcttc tcaattttag aacgccgctt
      181 tctcgatgtc agccaaccaa tgaccgattc ttactccccc ctcacccagt cacctggcct
      241 catcgttacc ggttggtgga acgaatattc acctcgtatt aatggaaaaa cggctgtcct
      301 caaacccaat gaaagatgtc gaagcttggc tatttcacct gatggccaat gggtaattct
      361 gggaactgaa tggtatctcc gctgtttaga tcgaaatggg caagaattat ggcattttcg
      421 cactcctgga gcctgttggg gggtcaatat atctcatgat ggattaaagg tagtcgctgc
      481 ttatggggat ggtacgctcc actggtaccg tttaaaagat gggcgtgaac tatttggtgc
      541 ttttttgctc cccgatcaaa atcattggac gatttggact cctcaaggat atttcgatgc
      601 cagtgaagga gcggatgagc ttattggatg gcatctcaac cagggaaagg atagtgcagc
      661 tgaattttat ccggcttccc gtttttttga ggaattctat cgtccttatc ttattcaaca
      721 aatcatagaa aaaactatga ccgatcaaga aatcattgag gaagaacaaa tccatcctcg
      781 ggtacaaata gaatccatca aaaaacctcc cctggttaaa attttatcgc ctcaaaacca
      841 acaaaccttt gatggagata ctgcagctgt tacctttgaa ataaccgatc agggtggtgg
      901 aatcgaagaa attttaatct atctgaacgg aaaacttttc aaccggataa ccgaaaacct
      961 gaaccaggaa aaaataaccc aaacagtcaa tttgaaactt ttaccaggcg acaatcagat
     1021 taaaatcaaa gccttaaacc aagaacatac cgaatccaat ccgactgaaa tccaggttca
     1081 ctgtaaaaaa gaaaagctcc ttcctaaatt atatatcctg gcagttgggg tgagtgagta
     1141 tgacgaccca caggtaaatg atctctttgc acctcgatat gacgctcaaa cctttgtggc
     1201 aacattagtg actcaacagg gattacttta ccgggaagtt attccaaccc tccttgtgaa
     1261 tgaggatgct acccgttctc gggtagtgga agcgatgaac actattatta accaagccca
     1321 agaagaggat gtggtaatat ttttttgggg tggtcatggc agttcccgtc aagaaggttg
     1381 ggatcccccc ttcttttatg ctttaccggc aaatgttttt gttgtgcctc gatctattta
     1441 tcaactcgat caagatatgg ttacctcaaa tctcatttct gattttttct atcgagtcaa
     1501 agccagaaaa caggccatca ttttagatac ttgtcactca ggtgctgcag tcggcattta
     1561 tcaacagggt cttttaggtc aagaacaagc ggttagggac ctttatcgaa atgttggagt
     1621 agcagtatta gctgcatcct cagcaactga aagtgcttac gaatcagtta tagtgaaagc
     1681 tgggtacttt actttttcac ttctctatgc cctgagtggt gatattatgc tcgattcaga
     1741 tcaaaaatca gccagcaagc tttatggatt tacctccaat gctgattata atcgagatgg
     1801 aaacataact atcaatgaag tttcaaagtt tttaaaagaa aaagtaccaa ctttagggga
     1861 ccaaacccct caaaccttca ctcaaggttt gaattttcct ttatatacta ccaaccagga
     1921 cataacacct ttcgatgaaa cattacagtt gatctttaca ccaccaaacg gaataaactt
     1981 tttacaggat attcaatatg gaacccgcta ttcaacccca aacaatggtg agctatattt
     2041 gtttgattac ccacctgagg ttgatattca agggcaagtc aaccagattg ttgccggcca
     2101 accatttcaa tgggaagtag cagtcaaggt cggagatgat aatgctatag ttaaagccta
     2161 tacttattct cgaaatcaga aaaactatgc tctgctgacc gcaatctatc caggaaccgg
     2221 tgtactcctt cttataatcg tcccatccga agaatatcaa tctgcccaat cctggatcat
     2281 gccagctatt actgaagtga aaattaagaa ataagggaaa attttaagtg tcattgataa
     2341 tacttcattc attactggat tttcaggaga aagtaaaaca tctctaaagg aggagtccat
     2401 tatatgaaaa gattaagatt cgtcatcttc attgctttca tgataacttg ggtttctgga
     2461 ttgggattgg cccaaaatag tggaaatatt ctcaatcttc ctggtgttgg ccaacctacc
     2521 ccttctaccg gtcaaactcc cggccagctc ccgactcttg atttctccca aagccaaccg
     2581 gcaactccca ctcagcaacc gacccttccc attccaagta cacctcaacc tggccaaatt
     2641 cagatccttg accccaacca aactcaaaat caaggacaac caatccctgc agtctggaaa
     2701 aaatataatc ttaaagacat agtcggtacc atggatcagg tgggaagagg ccaaatcgaa
     2761 tatccggaaa actggcaggt tattcctgat agtttcaatc ggatggttag tttgaatgag
     2821 gatgccagtg gtttagtatc ttttaggctt tacatcatta gttacgtgag atattccaat
     2881 gccaccgatt atgttgccgc cattgtccaa atgctttcct caactgtttc taatattcga
     2941 gttatcaata aggacgagca aaatatcacc aaccaagcgg tagcttccta tggggcgagc
     3001 gaaacaattg ctcgttatga actacaggga aatttccaag gaaccgagat gacaatgtgt
     3061 atcgaaacct gggtatattc gctttacgag agcaatttag gtaatgctgt tatatattgg
     3121 gctccaacct cagcttatca gcaaaaatat aaagactttt ttagccggat gatgacttct
     3181 tataaaaatt ccttaaaata aaaaaagagg catggggtaa gctcttttat tgattcccca
     3241 tgcctacagt tcataccgct taaaagatta taaacttttg gctttctgaa taatattttc
     3301 tactgagaaa ccaaatttct ccatgaccag ctcaccagga gccgatgcac caaatcgatt
     3361 cactccaata acaattccct gcgtcccaac atatctttcc catccaattg gaactccagc
     3421 ttcaattgaa atccgtttct taaccgatag cgggagaact tgatgacggt attcctccga
     3481 ttgctgctca aaaagttccc aggatggcat actgaccact cgaactttgg ttccctcagc
     3541 tgtcattttt tcgtaagctt ttaaagcaag tccaacctca gaaccggatg caatcaaaat
     3601 aagatcagga gttgaagaat cacaatcagc aagaacataa gctcctcggg cgaccccttc
     3661 agctgatgcg tacttggttt gatcgatcac cggtagtttc tggcgggaga aaattaaagc
     3721 caccggactt ttggcaccaa ccgctatttt ccaggcttga gcggtttcgt tggcatcagc
     3781 gggacggata accattaagt taggaactgt tcttaagctg ctgatatgtt ctactggttg
     3841 atgggttggt ccatcttcac cgag
//
LOCUS       DAOSOL010000119         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_23665_length_3864_cov_3.145445, whole genome shotgun sequence.
ACCESSION   DAOSOL010000119 DAOSOL010000000
VERSION     DAOSOL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23665_length_3864_cov_3.145445"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PK171_03875"
     CDS             complement(<1..255)
                     /locus_tag="PK171_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05362.1"
                     /translation="MFYIYEVMMMSKRMQERFSLSSLAKTLYEQGRRPIVPLMGYPGL
                     QLTKTTIKQNQFNHIVQFQSLSRLYDRFQPDSMFFMMDLSV"
     gene            complement(426..704)
                     /locus_tag="PK171_03880"
     CDS             complement(426..704)
                     /locus_tag="PK171_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOT05363.1"
                     /translation="MRRRKTTPNKSLKLLNNARGQLDAVIRLIHDDAAHVDILKQILA
                     TISLLRRSHTEILKKYLEECLDEDNPNGNGENRTKALAEIMKYMEKNF"
     gene            complement(701..1093)
                     /locus_tag="PK171_03885"
     CDS             complement(701..1093)
                     /locus_tag="PK171_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT05364.1"
                     /translation="MFTEIQLQTRKQEEFISITRILREAVEKSGTKDGIVIIQVPHTT
                     AGITINESADPDVVNDIVRMLNVMIPDRFEYLHQEGNSPAHIKASLLGSSALVIIKEG
                     DLMLGTWQGVFFCEFDGPRSRKIWVKII"
     gene            1658..2776
                     /locus_tag="PK171_03890"
     CDS             1658..2776
                     /locus_tag="PK171_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT05365.1"
                     /translation="MSSPVLFVSLRADNERNSVLQKVKRMTGQLLDKRLKSGDLVAVK
                     LHFGERGNHGFIQPVFLRYIIEEVKERGGKPFLTDTNTLYTGFRHNAVDHLETATFHG
                     FHYATVPAPLIIADGLRGSDFFEIEINQKHFKKVKVASAIHEADFLLVVTHVKGHVEA
                     GLGGSIKNIGMGCVARSGKQMQHGETFIPNPDSQKCIGCRRCIIHCPTQALSLDKNHK
                     AKVQKDLCIGCAECVVYCPTGAIAISWTSSASLLQERMVEHAFGVLKDKMTKSLFINF
                     LTDISPDCDCCDWHDASLVPDIGILGSHDLVAIDLASADLINRSIGLRDSSLSRAFQG
                     GQDKFLDVHPVANWRTQIDYAQLIGLGNTDYSLKEIIK"
     gene            complement(3110..>3864)
                     /locus_tag="PK171_03895"
     CDS             complement(3110..>3864)
                     /locus_tag="PK171_03895"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05366.1"
                     /translation="GSSVSPECQIDSIAQSWSILSGAGDIERSKTAMESVNQHLVHRD
                     QSLIQLLNPPFDKSNLNPGYIKGYVPGVRENGGQYTHGAIWVVMAFAAMGDQQKTWEL
                     FSIINPINHGKTKDEINIYKVEPYVVASDVYALPPHAGRGGWSWYTGSAGWMYQLIVE
                     SILGLRLDVDKLRFTPCFPVDWKQFIVHYRYRETVYHITFICSSGETRVIVDGIEQPD
                     QFVQLIDDHQEHSVEVIVPGSKINFVAGDEPN"
BASE COUNT         1155 a          752 c          713 g         1244 t
ORIGIN      
        1 aacagaaaga tccatcataa aaaacatgga atcgggttga aaacgatcat aaagtcggct
       61 gagtgattga aactgtacga tatgattaaa ctgattttgt tttattgtag ttttagttaa
      121 ttggagacct ggataaccca ttaaaggaac aataggtctc cttccttgct cgtaaagtgt
      181 tttcgctaac gatgaaagag agaaccgttc ctgcatcctt tttgacatca tcatcacctc
      241 atatatataa aacaataaac tatgtttata ctatatactc ggctcatgat tttgtcaata
      301 attatataaa atatttatat aattattata ggtgctttat aaaggttatt ttatctattt
      361 attttaaata tgaccgattt tataatcagt atatatttat tattcattta tatataacaa
      421 ttgagttaaa aattcttttc catatacttc ataatttctg ccaaagcttt ggttcggttt
      481 tctccattcc cgttcgggtt atcttcgtca agacattcct ccaggtattt ttttaaaatt
      541 tcagtgtgag aacggcggag taaagaaata gtcgctaata tttgtttcaa aatatcaaca
      601 tgagctgcgt cgtcatgaat aagacgaata acagcatcca gttgccctcg tgcgttattt
      661 aataacttta agcttttatt gggtgtagtt ttgcgtcttc tcatattatt ttcacccaaa
      721 ttttccgaga acgaggacca tcaaattcac agaaaaatac tccttgccaa gtcccgagca
      781 ttaaatcgcc ctcctttatt attaccagcg ctgaagaccc caataacgag gctttaatgt
      841 gggctggaga atttccttct tgatgtaaat attcaaaacg atcggggatc ataacattta
      901 acatccttac aatatcattc actacatcgg gatcggcgct ttcatttata gttattcctg
      961 cagttgtatg gggaacctga ataattacga tgccatcttt tgtccccgat ttttctactg
     1021 cttcacgtaa tatccttgtt atagaaataa actcttcttg ctttctggtt tgtaattgaa
     1081 tttcggtaaa cactctttct tctccttccc tttatttcaa tttatagatt cttctggttt
     1141 ccattcagga gttatattta agaagagtca ttctaaaata acagtaactc agaaaaagat
     1201 gagtcaaaaa gtccattttt gtttacaatc aaccacgaga ccctttacaa cctaatcctc
     1261 tcgaaaaaga attaaaacat gaaggaaggg ctgcttagag gaaaagagct catacacaaa
     1321 aaaaacaaag cgatctctaa aactaaccga tttaatgcct gataatatat tttttggttg
     1381 aactgccatt ttttaagtca gaacagtttg aaagcgagtt ttccctgttt ttttgctaat
     1441 cggtttcttg acttggtcta caagagacga aattcagata ccaagcggta gcactccaca
     1501 atattgcggt taccccatcc acacacccct ggttttcatt ctatcatatc ccgaaggaag
     1561 tcaacatgaa attaaagttg aaaattgttt tttatcccag ttccttctga tatactgaaa
     1621 aagtgatggt tattgtttta tgcctggagg tgtttgaatg tcgtctcccg ttttatttgt
     1681 tagtttgaga gctgataatg aaagaaattc ggttctacag aaagttaaaa gaatgaccgg
     1741 ccaactcctt gataagcgct taaaatctgg tgatttggtt gcagttaaac tccatttcgg
     1801 agaaagagga aaccatggtt ttatccaacc ggtattttta cgctatatta ttgaagaagt
     1861 taaagaaagg ggagggaagc cttttctcac cgataccaac actctttata ccggctttcg
     1921 tcataatgcg gttgatcatc ttgaaactgc aacatttcat ggattccatt atgcaaccgt
     1981 tccagctccc ctcatcattg ctgatggtct tcggggaagt gatttttttg aaattgaaat
     2041 taatcaaaaa cattttaaaa aagttaaggt cgcttcggcg atccatgaag ctgatttttt
     2101 actggtcgtt acccacgtaa aaggacatgt tgaagctggg ttaggtggat caatcaaaaa
     2161 catcggcatg ggttgtgttg ctcgttccgg aaagcaaatg caacatggag aaactttcat
     2221 tcctaatccc gattcgcaaa aatgcattgg ttgtaggcgc tgtatcattc attgtccaac
     2281 ccaagctctt tcccttgata aaaatcataa agcaaaagtt caaaaagacc tttgtattgg
     2341 ctgtgctgaa tgtgtagttt attgtccaac tggtgccatc gctatctcct ggacatcttc
     2401 agcgtcactt ttacaagaaa gaatggttga acatgccttt ggggttttaa aagataaaat
     2461 gactaagagt ctttttatta attttttaac cgatattagt cccgattgtg attgttgtga
     2521 ttggcatgat gccagtttgg ttcccgatat cggtatttta ggaagtcatg atttggtggc
     2581 tatcgatttg gcttcggccg atctcattaa ccgctcgata ggacttcgag actcgtcgct
     2641 aagtcgtgca tttcagggtg gtcaagataa gtttctcgac gtccatcccg ttgcgaattg
     2701 gaggactcag attgactatg ctcagttaat cggtttagga aatactgatt attctttgaa
     2761 ggaaataata aaatagagta gggaatctcg atgagattcc ctactctatt cgaactgagg
     2821 aataagaagt taagttaatt agcaaaagct gctttaatac aacaccaaga gagccttttt
     2881 gcattgatcg cgaagatttc tttcaatgct gggtgatccg acgatacgat atcaggcctt
     2941 gaaatccaag cgattaaata ttcgaaagct taccctttta ttccgacaac gaaagagtca
     3001 agaaatagct cttattctcg ctttgaaaaa agaggttttc ctgctgtact aaggaaagct
     3061 tctttttaaa tgtagggaaa atatttagaa tgagtccttc tctaaggtcc taatttggtt
     3121 catccccagc aacaaaatta atctttgaac ctggaactat tacctcaacc gaatgctctt
     3181 ggtggtcgtc aatcaattga acaaattggt cgggttgctc aatgccgtca actataactc
     3241 tcgtttcgcc tgaagaacat ataaaagtga tatgataaac cgtttctcga tatctataat
     3301 ggacaataaa ttgtttccaa tctaccggga aacaaggtgt aaaacgaagt ttatccacat
     3361 caagtctcaa ccctaggatt gactcaacta tcaactgata catccagcct gctgaacccg
     3421 tgtaccacga ccaaccccct cggccggcat gaggtgggag cgcataaaca tcagaagcta
     3481 ccacgtatgg ttctactttg tatatattaa tttcatcctt tgtttttccg tgatttatgg
     3541 gattgatgat agaaaataat tcccaagttt tttgttggtc acccatggca gcgaatgcca
     3601 taaccaccca gattgctcca tgagtgtatt gcccgccatt ttccctcaca cctggaacgt
     3661 atccttttat ataaccagga ttcaaattcg atttatcaaa cggggggttc aaaagttgta
     3721 tcagggattg gtcacggtga accagatgct ggttcaccga ttccatagca gtctttgacc
     3781 gctcaatatc accagctccc gagagaatag accagctttg agcaatcgaa tcaatctggc
     3841 attcagggct gactgatgaa ccaa
//
LOCUS       DAOSOL010000120         3848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_23772_length_3848_cov_4.435276, whole genome shotgun sequence.
ACCESSION   DAOSOL010000120 DAOSOL010000000
VERSION     DAOSOL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23772_length_3848_cov_4.435276"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            454..1746
                     /locus_tag="PK171_03900"
     CDS             454..1746
                     /locus_tag="PK171_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT05367.1"
                     /translation="MFGFSKSKWCTKKTAFFLTVMFIFLVLTPLASATKIQWLAHPVH
                     FAVTGDGELLKQFQEKTGIEVVPVLYGVDVIMEKAMMEMLSGSSTFDVISLSNTTWRN
                     DLGSDVFLELDSRISEIEDYDDYVPGLIEAFRAADGKLVALPIRSGAAMLHYREDLYN
                     EYGLAVPVTIDEFVSNAQALTKDTTGDGVVDVYGYAFMGKEGSQLIDDFESWLYPHGG
                     AFFDGDKVVVNNEAGVEAAALLTDLLNVYKVVPAGTLSYTSSEVKTAMQEGLAAMTTL
                     WWQYNIDWNKPELSKIAGNARMAKLPQKPDKGLGYGHMGCWAVFIDKNCKEVDAAWEF
                     IKFISSAEAQKYMVARGNGPTRLSSFQSEEFINAVGPEAAQIIADVYAVGKMPSPSPI
                     FPEARETVARELHQAFTGAKTPKQAMDDAASAIQKLIQ"
     gene            1845..2720
                     /locus_tag="PK171_03905"
     CDS             1845..2720
                     /locus_tag="PK171_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05368.1"
                     /translation="MASRKFNKSIPYLLILPALAIIFPLLIYPVFYNVYLSFFSWKVL
                     PPAFNFVKLQNYLDVFNNPVFWNSVKITLQFTGIVVGIQFVLGLGLAYLLNGQKFAKQ
                     TTRSIVLIPYISAPAIISLIWRLLWDPDLGQINQILRFFGIRGPGWIADPATSLFSVT
                     VTEIWRGIPFVILVLLAGLQALPDEPYDAAKVDGASSVQIFFLITVPLLKTIIWIVLL
                     FQTIFTLRAFDIIWVLTGGGPGGSTQTLSIMIYRTMFRFWDGGTSSTLSVIILILTLL
                     ISFVFFRYLYKEMEV"
     gene            2717..3517
                     /locus_tag="PK171_03910"
     CDS             2717..3517
                     /locus_tag="PK171_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05369.1"
                     /translation="MSRKTEGIIIILVVLFIAPVIQMVLMSFKQPLDLFSTFLFFKPT
                     GANYVDLFSRLNLTYFFKNSIIIASGTAVLSVILGAVAAYSFARYNFPMRKFLLFMVL
                     FSRMLPPVAGVVPLFLVMRRLGFTDTYGGIILLYTAFQTPFVIWMMRGFFLSIPRELE
                     EAAVIDGCSRLTAFLRITLPLSRPGLAATSIFAFTLSWNEFLFALIFTGTNTKTIPVA
                     VPELIGEMGIFWGQICAAGTLAVLPIFIFSFLAQKQLISGLTFGAVKG"
BASE COUNT         1016 a          828 c          818 g         1186 t
ORIGIN      
        1 ttttaggttt tttattcttc ataaatactt taaaggatga gattctcacg tcgcataaga
       61 cgctcctcag aatgacggag taggtggatg agattctcac gtcgcacaag tcgctcctca
      121 gaatgactga gaggccggat gaaattctct cgtcgtccgg tagaaaacac caggctcctt
      181 agaatgacaa actgggtggt agagattgcc acgtcgcata agacgctcct cgcaatgacg
      241 aaacagtaaa taattcaaat ccccctgacc ctcttttcta aagggggaaa cgattcctgg
      301 gtaagtttct tcatcctcat ctggcaccac gaaagtggca tgatagttta ttctaataag
      361 gaggtgataa gcatatttct gctgaaaata aaaataaatt agggcttcat cttggttccc
      421 cgattttcca aaatatttaa ggaggtagaa gaaatgtttg gtttttctaa atcgaaatgg
      481 tgtaccaaaa agacagcgtt tttccttacg gtaatgttca ttttcctggt tttaacccct
      541 ttggcttcag cgactaaaat tcaatggctg gcccacccag ttcatttcgc tgttaccggc
      601 gatggtgagt tgctcaagca attccaggaa aagactggaa ttgaagttgt accggtgctc
      661 tacggagtag acgtcatcat ggagaaagcc atgatggaaa tgctatcagg aagcagcacc
      721 tttgatgtta tctccttatc caatacgact tggcgcaacg atttaggatc ggatgtcttc
      781 cttgaacttg attcccgcat ttctgaaatt gaggattatg atgattacgt acccggtctc
      841 atcgaagcct tccgggctgc agacggaaaa ttggttgctt tgccgatcag atccggcgca
      901 gctatgcttc attatcgaga agatctctat aatgaatacg gattagcagt gccagtaacc
      961 atagatgaat ttgtcagcaa tgctcaagct ctcaccaagg atacaaccgg agatggggtt
     1021 gtggatgttt atggatacgc cttcatggga aaagagggat ctcagcttat tgatgatttt
     1081 gaaagctggc tttatcccca tggcggtgct ttttttgatg gtgacaaggt tgtagtgaat
     1141 aatgaagccg gtgtcgaagc cgccgcttta ctgaccgatc ttctcaatgt ctataaggtg
     1201 gttcccgccg gaaccctttc ctatacctcc agtgaagtca aaactgccat gcaggaagga
     1261 ctggctgcaa tgaccaccct gtggtggcaa tataatattg attggaacaa accagaactt
     1321 tctaaaatcg ctggtaatgc acgaatggct aaacttccgc aaaaacccga taaaggattg
     1381 ggatatgggc atatgggttg ctgggcagta tttattgata aaaactgcaa agaagtcgat
     1441 gctgcttggg aattcattaa gtttatctcc agtgctgaag cccaaaaata catggtcgct
     1501 cgtggaaatg gtccaactcg tttgtcaagc ttccagagtg aggaatttat caatgcagta
     1561 ggaccagaag ccgctcagat cattgccgat gtgtacgccg tcgggaaaat gccttctccc
     1621 tctccaatct tccccgaggc tcgagaaaca gtcgctcgtg agcttcacca agcatttacc
     1681 ggtgccaaaa cccccaaaca ggcaatggat gacgccgcta gcgcaattca gaaactcatt
     1741 caataaaatt ttataacatc atctcccact cggtactaac ctatgccgag tgggagataa
     1801 acaatttcaa aaagtagctt agatctggct ttggaggcta ttgaatggct tcaagaaaat
     1861 ttaataaaag tattccttac ttgctgatac tcccagctct cgctattatt ttccctttgt
     1921 tgatttatcc ggttttttat aatgtctacc tcagtttttt ttcctggaaa gttctccccc
     1981 cagcttttaa ttttgtcaaa ctgcaaaatt atcttgatgt gtttaataac ccggtatttt
     2041 ggaattcggt gaaaataact cttcaattca ccggaattgt agttggaatc caatttgtcc
     2101 ttggtttggg cttggcttac cttcttaatg gtcaaaaatt tgccaaacaa accactcgtt
     2161 ccattgtact catcccctat atcagtgctc ctgccataat cagcttaatc tggcgtctcc
     2221 tctgggatcc ggacctgggc caaattaatc agattctccg ttttttcggt ataagaggac
     2281 cgggatggat tgccgatccg gcaacctcgc ttttttcggt cacggtaact gaaatttgga
     2341 gggggattcc ttttgtcatt ttggtccttc tcgctggtct tcaagcttta cccgatgaac
     2401 cctatgacgc tgccaaagtc gatggagcat cgtcagtaca aatatttttt ctcattaccg
     2461 taccgctttt aaaaaccatt atttggattg ttcttctttt tcaaactatt ttcaccttgc
     2521 gggctttcga tatcatttgg gttttaaccg gtggtggacc gggtggttca acccaaactc
     2581 tcagcatcat gatttatcga acgatgtttc ggttctggga tggtggaaca tcttcaactc
     2641 tttcagtgat cattcttatc ctcaccctgc tgattagttt tgttttcttc cgatatttgt
     2701 ataaggagat ggaagtatga gccgaaaaac agaaggcatc attattatcc tggttgttct
     2761 ctttattgca ccggtcattc aaatggtctt gatgtctttc aaacaacctt tagacttgtt
     2821 ttcaactttt ttgtttttta aacctaccgg tgccaattat gttgatctgt tcagccggct
     2881 caatctcacc tactttttta aaaatagtat cattatcgcc tcagggacgg cagtattatc
     2941 ggtgatttta ggggcagttg ctgcctatag tttcgcccga tacaattttc cgatgcgaaa
     3001 attcctcctc tttatggtcc ttttttctcg gatgcttcca ccggtagccg gtgtggttcc
     3061 gcttttttta gtcatgagga gattgggttt caccgatacc tatggaggga tcattctttt
     3121 atatactgct tttcaaaccc cctttgttat ctggatgatg cggggctttt ttctgtccat
     3181 tccgagagaa ctggaagaag cagcagttat agatggatgt tcgcgactga ctgctttttt
     3241 acgaatcact cttcctctca gtcggccagg tttagctgcg acttcgattt ttgcttttac
     3301 tctttcttgg aatgaatttc tcttcgcctt aatattcacc ggtaccaata ctaaaactat
     3361 accagtcgca gtaccagagc tgatcggtga aatgggtata ttctggggtc aaatctgtgc
     3421 ggcaggaact ctggcggttc tgccaatttt tatcttttca tttttagctc aaaaacaact
     3481 catcagtggt cttactttcg gagcagtaaa agggtagacc tttttatttc gtttcgcaaa
     3541 tcaaaaccca tccccaagaa cccattgaat aatcaaacct aggggtagac ctgtgtgtct
     3601 gccctaatga aaaaaataga gaaagattaa aagacaataa attcattgga taaacaaaaa
     3661 aggagcggac tcaaagatcc gcccctacga taaatcgaaa aaaaatgatg aaaggggagt
     3721 ggtctgtcct gaaaatacac taatgcttaa aattgccgtc atcctgagcg gtgctttccc
     3781 gcatgaggat ctcatctttt aatgttatgg aattgggtaa tcgtggaact gcgaaacgga
     3841 aaaagatg
//
LOCUS       DAOSOL010000121         3821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_23992_length_3821_cov_5.499203, whole genome shotgun sequence.
ACCESSION   DAOSOL010000121 DAOSOL010000000
VERSION     DAOSOL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23992_length_3821_cov_5.499203"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK171_03915"
     CDS             complement(<1..652)
                     /locus_tag="PK171_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT05370.1"
                     /translation="MEFIEIFEMKMPVIGLGTWNLRGKECFEATISALEIGYRHIDTA
                     EMYENESEIGEAIAQSLVPRNEIFLTTKVWSTHLQYQDVILSLENSLKKLKTDYVDLL
                     LIHWPNPNISLSETFKAMNYLKNKEMLRFIGLSNFNKDLLMKAQKISFLPIMNVQVEY
                     HPFLDQNSILSYCQKNNISLTAYCPIARGRDLNNPTLEKIASKYEKTVPQIMLRWLI"
     gene            complement(737..937)
                     /locus_tag="PK171_03920"
     CDS             complement(737..937)
                     /locus_tag="PK171_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOT05371.1"
                     /translation="MKLKIEGMSCQHCVARVQKALQEVPGVLAVSVDLKKGEADITVK
                     DGVTPQSLISAIKNAGYESKVI"
     gene            complement(992..2143)
                     /locus_tag="PK171_03925"
     CDS             complement(992..2143)
                     /locus_tag="PK171_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT05372.1"
                     /translation="MKKYLFRIVLILIVLFILSNISFSQADLVSLVKKVKPTIVAIYT
                     FNNQGQSLMQGSGFFINNQGDVITNWHVIESAHSAVVKTFEGNSYLVKEALGGSKESD
                     LALLSVDIRNDSVQGLPISHSTPEIGERVLVMGNPLALEFSVSDGIIAAIRNIPEYSN
                     GEVIQITAPVSPGSSGSPVMNMQGEVIAIAFYVYFHGENLNFAIPSQRVIDIINNKRP
                     NPISLAQLSAVDFKIFEDSSHRFFVSYPSNWEPVTQNYQNQIINGFLAPTDSQNDSFR
                     ENLNVIVETYQSPVQLADYYQWNINQLKAFPDIQFLGNYDGFVSGVPSKILIYSRSQS
                     GKNLTFSQVYLVQGNKGYILTFTAESNRYQVYQPIFQQMMDRFRLNTNF"
     gene            2401..>3821
                     /locus_tag="PK171_03930"
     CDS             2401..>3821
                     /locus_tag="PK171_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT05373.1"
                     /translation="MTYRGFSKKRNIFIPLLVCIGYFLIFSPFNADASDTGEKEIRLT
                     LSQVVEFALATDSQILDAQDNVKVAQSNRRIAQKEKGFNPTVQVTGDIALVGEAESNA
                     LLSISDSIALNETSSDLANQLEQADLALNQAQNALKNAQENTKLKAITAYLEVLKAEW
                     TIEMAQRTLEQAHTLQADIENQYQLGMASSVDLLKAKQTVERARINLDQSNQSLLFKK
                     QQLNQMLGYPLDTPITLEKDFPYQPLGEELDQFTTHAQSDHTDLKDLLWQREIETITL
                     KQIERNRQAKIHLIGSYVEENYAVRFDLQSPDWVLDWKVTGQLSEGEDTFSTSAINQD
                     PFTPSASGWGLGLEVTWIPFDGGISQERKQQQEIILAQIERKLQALPDSITLEVLDAY
                     QLFIQSDQDTLTAQLEMQIAEETYRLQSQQYQAGFITDRTLKESELALESAKLDYQKA
                     VYGFILSKAQLYQIAGKEIVVEEL"
BASE COUNT         1182 a          795 c          714 g         1130 t
ORIGIN      
        1 ggattaacca acggagcata atttgtggaa ctgttttctc atatttgcta gcaatttttt
       61 ccaaggttgg attattaagg tcccgacccc gggcaatggg acagtaagca gttaatgaaa
      121 tattattttt ttgacagtaa ctcaatatgg aattttggtc gagaaaggga tggtattcaa
      181 cctggacatt cattataggc aaaaaagata ttttttgggc tttcatgaga agatctttat
      241 taaaattgct tagtccaata aatctaagca tctccttatt ttttagatag ttcatcgctt
      301 taaaagtctc agataaggag atatttgggt tcggccaatg aatgagtaac aagtcaacat
      361 aatcggtttt cagttttttt aaactatttt ctaaagaaag gattacatct tggtattgaa
      421 gatgagtgga ccaaactttg gtagttagaa atatttcgtt acgaggaacc aacgattgag
      481 ctatggcttc cccaatttca ctctcgtttt catacatttc agcagtatcg atatgtcggt
      541 aaccaatctc aagggcagaa atggttgctt caaaacattc tttccctctc aaattccaag
      601 ttccaagacc aatcactggc attttcattt caaatatttc aataaattcc atcccgtttc
      661 ctccttcttg ctaaattttt attctggcat agtattgtca agaatcgcaa tggtctatca
      721 aatttatacc gaggctttat ataactttgg attcataacc ggcattttta attgccgaaa
      781 tcagggattg aggcgtaact ccatctttga cggttatatc ggcttcaccc ttttttaaat
      841 cgacactaac agctaaaaca ccaggaactt cctgaagcgc tttttgtact ctcgcgacac
      901 aatgttggca actcatgcct tcgattttta atttcattta aaacacccca gaattttaaa
      961 atattagttt taaataatat ggaaaattta tttaaaaatt agtatttaat cggaaacgat
     1021 ccatcatttg ttggaaaatt ggttgataaa cttggtacct attagattct gcagtgaagg
     1081 ttaaaatata tcctttattt ccttgaacca aataaacttg tgaaaaagtc aggttttttc
     1141 cgctttgaga acgactataa attagtattt tgctgggaac acccgaaaca aaaccatcat
     1201 aattccctaa aaattggata tcggggaaag cttttaattg attgatattc cattggtaat
     1261 aatcagctaa ttgaactggg gattgatagg tttcaacaat aacatttaga ttttcacgaa
     1321 aactatcatt ttgactgtct gttggggcaa ggaagccatt gataatctgg ttttgataat
     1381 tctgggtaac cggttcccaa ttggaaggat aactaacaaa aaatctatgt gaagaatcct
     1441 caaaaatttt aaaatccaca gctgataatt gagccaaaga aattggatta ggtcttttat
     1501 tattaataat atctatcact ctttgactgg gaatagcaaa attaagattt tctccgtgaa
     1561 aatagacata aaaagcaata gcgattactt caccctgcat attcatcact ggactcccgc
     1621 tggaaccagg agaaaccgga gcggttattt gtatgacttc tccatttgag tattcgggaa
     1681 tatttcttat tgcagcaata attccatccg atacgctaaa ctcaagagct aaaggatttc
     1741 ccatgaccaa taccctttca ccaatttcag gggttgaatg agaaattgga agaccttgaa
     1801 ctgaatcatt tcggatatct actgaaagta aagccaagtc actttctttg cttccaccta
     1861 gagcctcttt tactaaatag gaattacctt caaaggtttt taccacggca ctatgagcac
     1921 tttcaataac gtgccaattg gtgatgacat ctccttggtt atttataaag aagccacttc
     1981 cttgcattaa gctttgacct tgattattaa atgtatagat agccacaatc gtaggtttta
     2041 ctttttttac cagagaaact agatcggctt gggaaaagga tatgtttgat aaaatgaaca
     2101 agacaattaa aattaatact attcggaaca aatatttttt cacaatttca ccctcctatg
     2161 tcccatgaat gaatacttat aattctatta tagaggattg atttgtgaat tgctcatttg
     2221 taatacgggt ttgcttatga tattctcaaa tgttatctaa atataaaata ttttatctaa
     2281 agagtcatcg aaacataagg aagatatgga atttcttttt attaattcag ataggtaaat
     2341 ttatccttta aaattattcc aataccgaac ttgttacccc caatgaggtg atgatcaatc
     2401 atgacatatc gaggatttag caaaaagagg aatatcttca ttcctctttt agtttgcatc
     2461 ggatattttc tcatcttttc tccttttaat gccgatgcca gtgatactgg agaaaaggag
     2521 atcaggttga ccctatccca ggtggtcgaa ttcgcccttg ctactgatag ccagatcctg
     2581 gatgcccagg ataacgtcaa agtcgcccaa tcgaatcgtc gtattgctca aaaagaaaaa
     2641 ggcttcaacc ccaccgttca agtcactgga gatatagcct tagttggaga ggcagaatca
     2701 aatgctctcc tttcaatcag tgactcgatt gccctgaatg agacctccag tgaccttgcc
     2761 aaccaattgg agcaggctga cctcgccctc aaccaagccc agaatgctct caagaatgcc
     2821 caggagaata ccaaactgaa agccatcacc gcttacctgg aagtcctcaa agctgaatgg
     2881 accatcgaga tggcccagcg cactcttgag caagctcata ctctccaggc tgatatagaa
     2941 aaccagtacc aattaggaat ggcctcatcg gtggatctcc tcaaggccaa acaaaccgta
     3001 gaaagagccc ggatcaacct cgatcaatcc aaccaaagtt tgcttttcaa aaaacagcag
     3061 ctcaatcaaa tgctcgggta cccgctcgat accccaatca ctctcgaaaa ggattttccc
     3121 taccagccac tgggggagga attggatcag ttcaccaccc acgctcaatc tgaccatact
     3181 gacctcaagg acttactctg gcaaagagaa atcgagacta tcaccttgaa gcagattgaa
     3241 cggaaccgtc aggccaaaat tcatctcatt ggatcctatg ttgaagaaaa ctatgcggtc
     3301 cggtttgacc tgcaaagccc cgattgggtc ctagattgga aggtcaccgg tcagctctcg
     3361 gaaggggagg ataccttttc aaccagcgcc atcaatcaag acccgtttac tccttcggca
     3421 tccggctggg gactgggatt agaagtaacc tggattccct ttgatggggg gatttcccaa
     3481 gaacgaaaac aacaacagga aatcatcctg gcccagatcg agagaaagtt gcaggctctg
     3541 cctgattcaa tcacccttga ggtattggat gcctaccagc tcttcatcca atccgatcag
     3601 gacactctca ccgctcagtt agaaatgcaa attgctgaag aaacctaccg actccaatcc
     3661 cagcagtatc aggctggttt tatcaccgac cgaaccctca aggagagtga attggctctg
     3721 gagagtgcca agctcgatta ccagaaggcg gtctatgggt ttatcctctc gaaggcccaa
     3781 ctctaccaga tagctgggaa ggagattgtt gttgaggaac t
//
LOCUS       DAOSOL010000122         3784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24298_length_3784_cov_4.352373, whole genome shotgun sequence.
ACCESSION   DAOSOL010000122 DAOSOL010000000
VERSION     DAOSOL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24298_length_3784_cov_4.352373"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK171_03935"
     CDS             <1..266
                     /locus_tag="PK171_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOT05374.1"
                     /translation="PSGSQQELCRGLHAEQNVIIQAALHGVSTKESILYCTHKPCSLC
                     AKMIINAGISRIVYQNPYPDPLGDELVSEAGLEVCIFKGGCES"
     gene            263..1321
                     /locus_tag="PK171_03940"
     CDS             263..1321
                     /locus_tag="PK171_03940"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT05375.1"
                     /translation="MTFLHLIVASLGAFFLSLLFTPLIMKFCQKKNLLDQPDGNRKIH
                     STPKSRLGGVAVAGSFFISLLVFRFFLSYPVSFWYILGLAAIFLTGFFDDIYNLPPWS
                     KVLGLGIGTLILIFDGVVIEFITLPWNVLWYIGIWGIPFTFFWILGITNSLNLIDGMD
                     GLSSGIAAIAACTLGIIALQHGRWESALISFLLMGSSMGFLPYNFPPAKIFIGDGGAL
                     FFGGVLATVSVEGALKSATTFTLAVPILILGIPILDTFFAIVRRKKNKLPITKPDRGH
                     LHHRLLEKGLTQREVILVVYLISASLSILAILIDRFLVNSTYSLLLTLVLFYVSWKWG
                     KHLGVTELSCGTQSTKKV"
     gene            1293..2453
                     /gene="wecB"
                     /locus_tag="PK171_03945"
     CDS             1293..2453
                     /gene="wecB"
                     /locus_tag="PK171_03945"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOT05376.1"
                     /translation="MGLKAPKRFKIAFVAGTRPEIIKTAPVYLAVARQPDLQPVFINT
                     GQHREMSHMFLNLFNIVSDYDLNIMKPEQSLSSLSASILQELERILQRENPDWVLVQG
                     DTTSAFCGALSAFYQKIAIGHIEAGLRTGDIYSPFPEEMNRILVGRLSTYHYAPTQKA
                     KDNLLNEGISPNKIMITGNTVVDALYWIQSQDKQISSSDISRLLSSNPNQRLVLVTAH
                     RRENWEGGIANVAEAVVRLAKRFPDIVFIFSVHPNPVVKDQVYAILSNQANVFLHDPL
                     DYFDFIKVLNQSLLAISDSGGVQEEAPSLGVPVIITRSTTERPEILGTGMGYLAGTDQ
                     TTIINLATELLTKKQARKAQNIFGDGFASQRIVQSILFSLGKIPKRPDDFDY"
     gene            complement(2454..2879)
                     /gene="atpC"
                     /locus_tag="PK171_03950"
     CDS             complement(2454..2879)
                     /gene="atpC"
                     /locus_tag="PK171_03950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOT05377.1"
                     /translation="MKKMELHIVTPEQTYIYPDVYSITLECPDGLRGILPGHAPFLVE
                     LLTGVLKYTTIDDQKKYLAISGGAAEITPYQVTIITDSAEGADTIDVARAQEAATRAQ
                     QRIKEKIPGTDFIRAEAALKRSLARLRAAELAGKTGRYS"
     gene            complement(2876..>3784)
                     /gene="atpD"
                     /locus_tag="PK171_03955"
     CDS             complement(2876..>3784)
                     /gene="atpD"
                     /locus_tag="PK171_03955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOT05378.1"
                     /translation="LIRRTATAHRGVSVFAGVGERVREGNELYLQMQKSGVLSHTVLV
                     FGQMNEPPGARFRVALTALTMAEYFRDVLSQDVLLFVDNIFRYVQAGSEVSALLGRLP
                     SAVGYQPTLAEEVGMVEERIASTINGSITSVQAVYVPADDLTDPAPATTFSHLDAITV
                     LSRKIFERGFYPAVDPVQSTSRALDPLIIGDTHWRLAQEARKIIAHYLELQDIIAILG
                     IDELSDEDKNIVNRARKLQRFFTQPFFAAENYTGIPGEFVSLEKTLTGVQNIIEGQCD
                     DWPEQAFYMTGTIEQVEARVKELKNE"
BASE COUNT         1057 a          783 c          778 g         1166 t
ORIGIN      
        1 ttccttccgg aagtcaacag gaactctgtc gagggttaca tgctgaacaa aatgttataa
       61 tacaagcagc tcttcatgga gtgagtacca aggaatcgat tttatattgt acgcacaaac
      121 cctgttcgct ttgtgctaaa atgattatta atgctgggat ttcgagaata gtctatcaaa
      181 atccctatcc tgatcccttg ggagatgagt tggtgagcga agcaggctta gaggtatgca
      241 tatttaaagg tgggtgtgaa tcgtgacatt ccttcattta atcgtggcaa gcttaggggc
      301 ttttttccta tcccttttat ttactccatt gataatgaag ttttgtcaaa aaaagaacct
      361 tcttgatcaa cccgatggaa accggaaaat ccatagcaca ccaaaatccc gtttaggggg
      421 agtggcggtt gccggttctt tttttatttc gcttctcgtt ttccgctttt ttctgtccta
      481 tccagtgtct ttttggtata ttttaggttt agcagcaatt tttctaaccg gattttttga
      541 tgatatttat aatttaccac cttggagcaa ggtattgggt ctcggaatcg ggactcttat
      601 tcttattttt gatggagtgg ttattgagtt tatcacttta ccgtggaatg ttctctggta
      661 cattgggatt tggggaattc cctttacttt cttctggata ttaggcatta ccaattcctt
      721 aaatcttatc gatggaatgg atggtttatc cagtggaatt gctgccattg cagcctgtac
      781 tttagggatt attgctcttc aacatgggag gtgggaatca gctttaatct cttttttgtt
      841 aatgggttca tcaatgggat ttcttccgta taacttccca ccagctaaaa tatttattgg
      901 tgatggagga gcactctttt ttgggggagt tttagcaacc gtatcggtag aaggagccct
      961 taaaagtgca accactttta ccttggccgt tcccattctt attctgggaa ttccaatact
     1021 cgataccttt tttgccatcg ttcgccgcaa aaagaataaa ctgcctataa ccaagccgga
     1081 tcgtggccat cttcatcatc gattattaga aaaaggctta actcaaaggg aagttatttt
     1141 agttgtatac ttgattagtg ctagcctcag tatccttgcc attttaattg accgcttttt
     1201 ggtgaatagc acctattctc tcctacttac cttagtgctc ttctatgtct cttggaaatg
     1261 gggaaaacat ttaggtgtga cggagctttc ttgtgggact caaagcacca aaaaggttta
     1321 aaatagcctt tgttgccgga actcgaccgg aaataatcaa aacagcacca gtttatcttg
     1381 ccgttgcccg ccaaccagat ttacagccgg tttttattaa taccggacaa caccgggaaa
     1441 tgtctcacat gtttttaaat ttgttcaata tagtttctga ttacgacctc aatattatga
     1501 aaccggaaca atcccttagc tcgttatctg catctattct tcaggaatta gaaaggattc
     1561 ttcagagaga aaatcccgat tgggttctgg ttcaaggtga tacgacttca gctttttgtg
     1621 gtgccttatc agccttttat caaaagatcg ctattggtca tattgaagct ggactccgta
     1681 ctggtgatat ctacagtccc tttcctgaag aaatgaatcg tattttagtt ggacgtttga
     1741 gcacctacca ttatgctcct acccagaaag caaaagataa ccttttaaat gaaggaattt
     1801 caccgaacaa gataatgata acagggaata ctgttgtaga tgccctttat tggattcaat
     1861 ctcaagataa gcagattagt tcttcagata taagtcgatt attatcatcc aatcccaatc
     1921 aaagattggt tctggtaact gctcatcgga gagaaaattg ggagggtggc attgcgaacg
     1981 tagcagaagc ggtagttcgt ttagccaagc gttttcctga tattgttttt attttttcag
     2041 ttcatcctaa tccggtggta aaggatcaag tatatgcaat tctttctaac caggctaacg
     2101 tttttcttca tgacccactg gattattttg attttataaa agtattaaac cagagtctgc
     2161 tggcgattag cgattcaggt ggtgtacaag aagaagctcc gtctttggga gttccggtta
     2221 taatcaccag gagtacgacc gaaagacctg aaatcctcgg aacaggtatg gggtatttag
     2281 ctggaaccga ccagactacc atcattaacc ttgctactga actattgaca aaaaaacaag
     2341 caagaaaagc tcaaaatatt tttggagacg gatttgcctc gcaaagaatt gttcaatcca
     2401 tccttttttc ccttggaaag ataccaaaaa gaccggatga ttttgattat taattatgaa
     2461 tatcgaccag tttttcctgc taattctgct gcccgtagcc gagccaatga acgcttaaga
     2521 gcggcttcag ctcgaataaa atctgtgcca ggaatttttt cttttatccg ttgctgggca
     2581 cgagtggcag cttcttgagc tcgagcaaca tcaatggtat cagcaccttc agcactatcg
     2641 gtaataatag tgacctgata aggtgtaatt tctgctgcgc caccgctaat cgcaaggtat
     2701 tttttctgat catcaatggt ggtatatttt aatacaccag ttagaagttc taccaaaaaa
     2761 ggagcatgtc caggaaggat tcctcttaat ccatcaggac attcaagagt aatcgaataa
     2821 acgtcgggat aaatataagt ttgctctggt gttacaatat ggagctccat ctttttcatt
     2881 cgttcttcaa ctccttcacc cgagcttcga cttgttcgat ggtgccggtc atataaaatg
     2941 cctgttcagg ccaatcatca cattgaccct cgataatgtt ttggacacca gttaaggtct
     3001 tttctaagga aacaaactct ccaggaattc cagtataatt ttcagctgca aaaaaaggtt
     3061 gtgtaaaaaa gcgctggagc tttcgggccc gattgacaat atttttatct tcgtcagaaa
     3121 gttcatcgat tcccaaaata gctataatat cctgcagctc aagataatga gcaataattt
     3181 tacgtgcttc ctgagctaac ctccaatggg tatccccaat aatcaaggga tcaagagctc
     3241 ttgaagtaga ttgcacggga tcgacggccg gataaaaccc tcgttcaaat atttttctag
     3301 aaagaacagt tatagcatca aggtgtgaaa aggtggtggc tggagctgga tcagtcaagt
     3361 catcggcagg aacataaaca gcttgaaccg aggtaataga tccattgatg gttgaggcaa
     3421 ttcgttcctc aaccattccc acttcttccg ccaacgtcgg ttgatagcca acagccgaag
     3481 gaagacgtcc caaaagagct gatacttccg atcccgcttg aacataacga aaaatattat
     3541 cgacaaataa caagacatct tgagaaagaa catcccgaaa atactctgcc atagtcagag
     3601 ccgtgagggc aacccgaaag cgagctccag ggggttcatt catttgacca aaaactaaaa
     3661 ccgtatggct tagtactccg cttttttgca tctgaagata gagttcgttg ccttcccgaa
     3721 ctctttctcc caccccagca aataccgata ccccgcgatg ggctgttgcc gtccgacgaa
     3781 tgag
//
LOCUS       DAOSOL010000123         3781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24324_length_3781_cov_3.529791, whole genome shotgun sequence.
ACCESSION   DAOSOL010000123 DAOSOL010000000
VERSION     DAOSOL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24324_length_3781_cov_3.529791"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PK171_03960"
     CDS             complement(<1..454)
                     /locus_tag="PK171_03960"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOT05379.1"
                     /translation="MAFSRQERIVFIILSSILVFSTGFLFYKTWTVSLPNKVSNENRP
                     ENYIIQIAGEVLKPGVYQVEEGTRLYQLIELAGGVSPQADISSLNLAAPVHDGLRINI
                     PAQNSNQGQLENSQLSFIGQRPLSEETDSNLVVQINTASLEELKRLPGI"
     gene            complement(625..3096)
                     /gene="leuS"
                     /locus_tag="PK171_03965"
     CDS             complement(625..3096)
                     /gene="leuS"
                     /locus_tag="PK171_03965"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT05380.1"
                     /translation="MRENYDFDAIELEWQKKWSDHRLFQVETNPERRKYYVLEMFPYP
                     SGDPHMGHVKNYVIGDVVARYFTRKGFNVLHPMGYDSFGLPAENAAIKNNIHPSVWTH
                     DKIDKMREVLKRIGISYDWRREVITCEPEYYKFTQWFFLQFYKNNLAYKKAGQVNWCP
                     SCATVLANEQVVEGNCERCGTPVIRKMLSQWYLKITQYAEALLNDIDTLGEWPERVLA
                     MQRNWIGRSEGAYIDFSLPDLNQSIRVFTTRPDTVFGVTFFVLAPEHSLVDELVAGTP
                     YQDEVIKFREKISRKSDIDRMSETLDKDGMYIGKEIVNPINGEKIPIWIADYVLLEYG
                     TGAVMAVPAHDERDYQFAVKYNLPIRQVIQPLQSDNKEKCYAGSGLMVNSGDYSGLPS
                     EEGKRKIISYIEEKEIGKGAVTYRLRDWLISRQRYWGAPIPILYCNRCGEVPVSEKDL
                     PVLLPKNVDFQPGGPSPLARSDEFVNTVCPICGGPAKRETDTMDTFMCSSWYFLRYCS
                     PWTDQEPFERKDVDYWMPVNQYIGGVEHAILHLLYSRFFIKVLRDLGMINFPEPFINL
                     FAQGMVTKGGVKMSKSKGNVVSPRDIILRYGADTVRVFILFAGPPELDMEWSDRGVEG
                     AHRFLNRVWRTVTEVIRCEEDQRVEPDQEKIKALERMIHRTILKVDTDIRERFHFNTA
                     ISSLMELLNEIIDSHRAFSGKGIHPQEKKILITAAKTLISLLNPFVPHLTEELWRALG
                     ETSFLSSSDWLTHDEEKLVENQVEIVIQINSKVRSKVTVSAGTDEKGVLQIALKDEKV
                     QTWIDQKTLVKHIFVPDKLLNLVVR"
     gene            complement(3153..3228)
                     /locus_tag="PK171_03970"
     tRNA            complement(3153..3228)
                     /locus_tag="PK171_03970"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3193..3195),aa:Ala,seq:ggc)
     gene            complement(3300..>3781)
                     /locus_tag="PK171_03975"
     CDS             complement(3300..>3781)
                     /locus_tag="PK171_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HOT05381.1"
                     /translation="AHPDREMTLRKRAQGLYNADRMSTIRKAQDNPSLKYLYEKWLEK
                     PNSEEAEKFLHTSYRSRRRISKEYIRIQEAKESQKIDIAVCVGTSCYLKGSYNLLQRL
                     LELAKEHDLQDRVSIGATFCLEKCSLGPNIRINDEVISGVTEQNVKEIFENHVLAKL"
BASE COUNT         1102 a          824 c          715 g         1140 t
ORIGIN      
        1 cgattccggg gagtcttttg agctcctcaa gggaagcagt gttgatttga acaaccagat
       61 tactatcagt ttcttcagat aaggggcgtt gcccaataaa agataattga gaattttcaa
      121 gctggccttg attagaattc tgagctggaa tgttgatacg gagtccatca tgtactggtg
      181 ctgccaaatt taaacttgaa atatcagctt gaggggagac tccgccagca agctcaataa
      241 gctggtatag ccgggtaccc tcttctactt ggtatactcc tggcttgagc acttcaccgg
      301 cgatttgaat aatatagttt tctggtctat tctcgttcga aaccttattc ggtaacgaaa
      361 cagtccaggt tttataaaat aaaaaaccgg tactaaacac taatattgac gatagaataa
      421 taaaaacgat tctttcttga cgagagaatg ccatcgttct tgctttaaac ctaataaaat
      481 ccccgctaat aaattttaat cttttgattt aatacgttag aaatctcata caataagcaa
      541 tttatttata ttcataatat gataatttct attataatat aaaataaata aaatggaaat
      601 aggattctct tgaagaaagg cgttttatcg aaccactaga ttcaaaagct tatctggaac
      661 aaagatatgt ttaaccagtg ttttttgatc tatccaagtt tgaacttttt catccttcag
      721 ggcgatttgc aaaactcctt tttcatcggt accagcagac acagtaacct tacttcttac
      781 tttactattg atctgaataa caatttcaac ttgattttcg accagtttct cttcatcgtg
      841 agttaaccaa tcagaggatg acaaaaagga cgtttctcct aaggctctcc agagttcttc
      901 ggtcaggtga ggaacaaaag gattaagcag cgatatcagg gttttagcgg ctgtaattaa
      961 aattttcttt tcttgaggat gaatcccttt tccggaaaaa gcccggtgag aatcgataat
     1021 ctcatttagc aattccatca atgaactgat agcagtattg aaatggaaac gctcacgaat
     1081 atcggtgtcc acttttaaaa tcgtccggtg gatcattcgt tccaaggctt taattttctc
     1141 ttgatcaggt tctactcgtt ggtcttcctc acatcgaata acttcagtta ctgttctcca
     1201 tacccggttg agaaatcgat gtgctccttc aacgcctcga tctgaccatt ccatatccag
     1261 ttctgggggc ccagcaaaaa gaataaagac ccgaacggta tctgccccat atctcagaat
     1321 tatgtctcga ggacttacca catttccctt tgattttgac attttaactc cgcccttggt
     1381 aaccattccc tgggcaaaca aattaataaa aggttcggga aaattgatca tccccagatc
     1441 tctcaatact tttataaaaa agcgtgaata aagaaggtgt agaatggcat gttctacccc
     1501 accaatatac tgattgactg gcatccaata atcaacatct tttctttcaa aaggttcttg
     1561 gtcggtccaa ggtgaacaat agcggagaaa ataccaggaa gaacacatga aggtatccat
     1621 ggtatcggtt tctctttttg ccggaccacc acaaatcggg caaacggtat tgacaaactc
     1681 atcactccga gctaatggtg aaggtccacc cggttggaag tcaacatttt taggaagaag
     1741 cacgggaagg tctttttctg aaaccggtac ttcaccacaa cgattacagt ataaaatggg
     1801 aataggagct ccccaatatc tttgccgaga aattaaccag tctcttaaac gataagtaac
     1861 cgctcccttg ccaatttctt tctcttctat ataggaaatt atttttcttt ttccttcttc
     1921 cgaaggcaaa ccactataat ctcctgaatt aaccattaat cctgaaccag cataacattt
     1981 ctctttgtta tccgactgaa gaggttggat aacttggcga atcggcaaat tatatttaac
     2041 tgcaaactgg taatcacgtt catcatgagc aggaaccgcc ataactgcac cagttccata
     2101 ttcaaggagc acgtagtcag ctatccagat cggaatcttc tccccgttaa tgggattcac
     2161 gatttccttt ccgatataca ttccgtcctt gtctaaggtt tccgacatcc gatcgatatc
     2221 gctttttcga gaaatttttt ctcgaaattt aataacttca tcttggtagg gtgttcctgc
     2281 aaccagctcg tcaaccaaag aatgttcggg agctaagaca aaaaaagtca caccaaagac
     2341 ggtatcaggc cgggtggtaa atactctgat tgactggttt aaatccggaa gagaaaaatc
     2401 tatgtaagca ccttcgctcc gaccaatcca attcctctgc atagcgagaa ctcgttccgg
     2461 ccattctcca agagtgtcga tatcattcaa caaagcctca gcatattggg tgatttttaa
     2521 ataccattgg ctaagcattt ttcgaataac cggtgttcca catcgttcac agttcccttc
     2581 cacaacctgt tcgttggcga gaactgtggc gcaagaagga caccaattta cttgaccggc
     2641 ttttttatag gctaaattgt ttttataaaa ttgcaggaaa aaccactgag taaatttata
     2701 gtattctggt tcacaggtta tgacttctct ccgccaatca taactgatac caattcgttt
     2761 taaaacttca cgcattttat ctattttatc atgagtccaa accgatggat gaatgttgtt
     2821 tttaattgca gcgttttccg caggcaaacc aaaagagtca tagcccatgg gatggagaac
     2881 attaaagcct ttgcgggtaa aataccgggc tacgacatct ccaatcacat aattcttaac
     2941 gtgacccata tgaggatcgc cagaaggata aggaaacatt tctaaaacat agtattttct
     3001 tcgttcgggg ttggtttcta cctgaaagag gcgatggtca gaccactttt tttgccattc
     3061 caactcaatt gcatcaaaat cataattctc tcgcaaactg cttaccccct atatggaaaa
     3121 agcccgtccc gtagggacga gctttattat actggtggag ctgaggggag ttgaacccct
     3181 ggcctcctcc acgccaagga ggcgctctcc caactgagct acagccccaa aattacttag
     3241 cagacgacct atattatagg agaatcacgt tcatcagtca acggattcat caaaaaagct
     3301 taaagtttag ctaagacatg gttttcaaat atttctttta cgttttgttc agttacccca
     3361 gagataactt catcgttaat ccgaatatta ggcccgagag agcacttttc taaacagaag
     3421 gtggcaccaa tactcactcg gtcttgcagg tcgtgctctt tggctaattc aagcaaacgc
     3481 tggagaaggt tataggaacc cttcaggtag cagctggttc caacacacac agcaatgtca
     3541 atcttctgag attctttagc ttcttgtatt cgaatatatt ctttgctgat tctccggcga
     3601 gaccgataac tggtatgaag gaatttttcg gcttcttcgc tattgggttt ttctaaccat
     3661 ttctcataga ggtattttaa tgatggatta tcctgggctt ttcgaatcgt gctcatccgg
     3721 tcggcattat agagaccctg agctcttttg cggagtgtca tttctcggtc gggatgggct
     3781 a
//
LOCUS       DAOSOL010000124         3761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24482_length_3761_cov_3.819212, whole genome shotgun sequence.
ACCESSION   DAOSOL010000124 DAOSOL010000000
VERSION     DAOSOL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24482_length_3761_cov_3.819212"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="PK171_03980"
     CDS             <1..354
                     /locus_tag="PK171_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05382.1"
                     /translation="GIPHSSKNKEAAWLFAQWVARKEWQGEFAKLGTRVVRNSTFEDP
                     IVIELDPQMGNYFTFLKEQGPLFRGAPALPMHRPLNDLYLKWVSKAVAGEISPDEALD
                     NLAKETDELMEELGY"
     gene            369..1238
                     /locus_tag="PK171_03985"
     CDS             369..1238
                     /locus_tag="PK171_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05383.1"
                     /translation="MRGKQKQVWGLLTPTLILLIFLGVVPIIYILYLSVYKYNVFSKV
                     GMVYTGFNNFRKLVFDPDFLKSLRLGLTYVILCVAIELPLGLAFANLLTYEYKGKGIF
                     RTIMTLPLAMAPISIGAIWVLMTNPDFGPLAVYLRNIGIDFNIGNNANQAFIATILMD
                     IWHWTPFVTLTFMAGLSSLPKQPFEAALVDGASKSQTLRYITLPLLRPVLFTTLFIRI
                     MDTFRVFDEVWMLTSGGPGTATRYVSIHVVREVLQSMNYGYSSAMSLFLLYLTIVVCW
                     LLITFINVSQESG"
     gene            1239..2072
                     /locus_tag="PK171_03990"
     CDS             1239..2072
                     /locus_tag="PK171_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05384.1"
                     /translation="MKASTKKTTRLFFIYLISILIAIATLFPIYWLFVVSARTKVEIF
                     AGPKLFQTSFYAVNYIRPFLHDVFGKYLLNSLIIASGNTLLVVTFAVLSTYAFSRFRV
                     PGSSNIFFWTITNRMAPPAVFIVPYFLIFTRLGLRDTQIGIILLYCIFNLPFAIWLLK
                     GMMDAIPREMDEAAYIDGCTFWGVLTKVILPLARPGIMTTAILTWIFAWNEYLFASIL
                     TSVNARTITTGLASFVTVIGTNWGEMAAVSMVCLIPAVIFIGIAQRHIVAGLTFGAVK
                     D"
     gene            2083..2253
                     /locus_tag="PK171_03995"
     CDS             2083..2253
                     /locus_tag="PK171_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05385.1"
                     /translation="MRIFPFKTNLWDRVFLSIVIMFAVHLFWVRFIETYAPLSIATVG
                     TLVFTAFVIIFG"
     gene            complement(2730..>3761)
                     /locus_tag="PK171_04000"
     CDS             complement(2730..>3761)
                     /locus_tag="PK171_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05386.1"
                     /translation="ICNNLRQYNIPFTLTSDHTEDPDSAIFKEDIATFAGVSRIVSGF
                     KNLKAGAIGARPNAFNTVRYSEKILENSGISVEVIDLSEILARIEKLKDSDQLVVNKV
                     KDIHNYINTSGIPDAPLVKMAKLWLVIDEWIKETGVKLFAFQCWSSIQENFGIMPCAV
                     MSMFSDNLIPAACEVDLTGGLAMYALQLASQKPSALVDWNNNVDDDPDKAILFHCSNY
                     PKSCLEDFRMGFGDIISTSAVSKEQAYGTCYGTIPSGPVTIARLATDDITGSITGYLA
                     EGNITADKFPTFGGIGVVEIEDLQDLLAFLCENGFEHHAAINLAHSANILYEAFDKYL
                     GFDTYWHNA"
BASE COUNT         1041 a          711 c          787 g         1222 t
ORIGIN      
        1 ggtatccctc atagctcaaa gaataaagaa gcagcttggt tgtttgctca gtgggttgct
       61 cgtaaagagt ggcagggtga attcgctaaa ctgggaacca gagtagtgag aaattcaact
      121 tttgaagatc caattgtcat agaattggat ccacagatgg gtaattattt caccttcctc
      181 aaagaacaag gtccactctt caggggtgct cctgcgctgc caatgcatag accgctaaat
      241 gacctttacc tcaaatgggt atccaaagca gtagctggag aaatttctcc tgatgaagcg
      301 ctcgataatt tagccaaaga aaccgacgag ctcatggaag aactgggtta ctaatagcgg
      361 aggatcaatt gagaggaaaa cagaaacagg tgtggggtct tttgaccccc accctcattt
      421 tattgatttt tctcggagtc gtcccaataa tttatatttt gtatttgagt gtttataaat
      481 ataatgtgtt ttctaaggta ggaatggttt ataccggttt taataatttt cgaaaactgg
      541 tatttgatcc ggatttttta aagtccttac gactggggct tacctacgtt atcctctgtg
      601 tagccattga acttcctctt ggtttagctt ttgccaatct tctcacttat gaatataaag
      661 ggaagggaat atttcgaact atcatgactc ttcctttggc tatggctcct ataagtattg
      721 gagcgatatg ggtgcttatg accaatcccg attttggtcc tttggcagtc tatttgcgaa
      781 atattggcat tgattttaat atcggtaaca atgccaatca agcttttata gctacgatat
      841 tgatggatat ctggcattgg actccttttg ttactcttac ctttatggca ggtttatcgt
      901 cccttcccaa gcaacctttt gaggcagcat tggttgatgg ggcttcaaaa tcgcaaactc
      961 tccgttatat taccttaccc ctattacgcc cagttctttt tactactctc tttatcagga
     1021 ttatggatac tttccgagtt ttcgatgaag tatggatgct taccagcggc ggaccaggga
     1081 ctgcaactcg ctatgtcagc atccatgtgg ttcgtgaagt tctccaatca atgaattacg
     1141 gctatagctc agcaatgtca ctttttttgc tttatttaac cattgtggtt tgttggctcc
     1201 ttataacctt tattaatgtt tcacaggaga gtggctaaat gaaagcatca accaagaaaa
     1261 ccacccgact ttttttcata tatttgattt ctattttgat agccattgca acacttttcc
     1321 cgatttattg gctttttgtg gtatcagctc gtaccaaagt ggaaatattc gctggcccta
     1381 agcttttcca aaccagtttt tatgcggtta attacattag gccttttttg catgatgttt
     1441 ttgggaaata tctactcaat tccctcataa tcgccagtgg gaataccctt ttagtggtaa
     1501 cctttgcggt cctttccact tacgcttttt cccggtttcg agttccagga tcgagcaata
     1561 tttttttctg gacgatcacc aaccgaatgg cccctccggc agtttttatt gtgccctatt
     1621 ttttaatatt tacccgtttg ggtttgagag atacccaaat tggtattatt cttctttatt
     1681 gtatttttaa tcttcctttt gctatttggc ttttaaaggg gatgatggac gcaatacccc
     1741 gggaaatgga tgaagcagcc tatattgatg gttgtacctt ctggggggtt ttaactaaag
     1801 tgatactccc cctggctcgt ccaggaataa tgacaactgc aattctgacc tggatatttg
     1861 cttggaatga atatctattt gcatcaattc ttaccagtgt gaatgctcga acgattacaa
     1921 ctggtttggc ttcttttgtt acagttattg ggaccaactg gggagaaatg gcagcggttt
     1981 ctatggtttg tttgattcct gccgtcattt tcattggaat tgctcaaagg catatagttg
     2041 ctggtctaac ttttggtgcg gttaaggatt aggtggaaaa atatgagaat attccctttt
     2101 aaaaccaact tatgggatcg agtattttta agtattgtta ttatgtttgc cgttcattta
     2161 ttctgggttc gttttattga aacctatgct cctttatcaa ttgcaacagt gggaacactg
     2221 gtatttacgg cttttgtgat aatttttggg tagatcagaa tcgatcctca tgtcactttc
     2281 atggcaccag atgaggatga aaatagttat ctgaatcctt cattgcaagg agtccaaccg
     2341 tttttttggt tggacgacgt ggtaatctca tttagttttt tttaaaataa acaaaagatg
     2401 agatcctcac gcccttgtaa agcgagggct caggatgaaa cctgcggcgt cagatgagat
     2461 cttcacgctc ttagatactg agggggagag tgagcgaggg ggtgaatgaa aaaagtatta
     2521 aaggataaaa aaaaaattaa aagatgagat cctcacgcgg gaaagcaccg ctcaggatga
     2581 cgtctttttt aaaatctttg gaaggggctt gatttatcaa gcttatagat gaatttttag
     2641 aattgaggaa gggaagatga atacaaaaaa agggaggtta tttgacctcc cttttttttt
     2701 ggttataaaa tttattcctg aaaatgaaat taagcgttgt gccaataggt atcaaaaccc
     2761 agatatttat caaaagcttc atacaaaatg ttagcagaat gagctaggtt gatggcggca
     2821 tgatgctcaa agccgttttc acagagaaac gccaagaggt cttgcagatc ttcgatttcg
     2881 acaacaccaa ttcctccaaa ggtggggaac ttgtcagcgg taatgtttcc ttcggcaagg
     2941 taaccagtaa tggaaccggt gatatcatca gtagcaaggc gggcaatggt aactggccca
     3001 gacggaatag taccatagca ggttccatag gcttgctctt ttgatactgc agaagtggag
     3061 attatatcac cgaatcccat gcgaaaatct tctaagcagg atttaggata attactgcag
     3121 tggaatagaa tggctttgtc ggggtcatca tcaacattat tattccagtc aaccaaggct
     3181 gatggctttt gagaagcaag ttgaagagca tacattgcca gtcctccagt taaatcgact
     3241 tcacaggcgg ctggaatcaa attatcgctg aacatgctca tcaccgcaca aggcatgatt
     3301 ccaaaatttt cttggataga ggaccagcat tgaaaagcaa aaagttttac accagtttct
     3361 ttgatccatt cgtcaataac caaccagagc tttgccattt taaccaatgg agcatctgga
     3421 atacctgaag tgttaatgta attgtgaatg tcttttactt tgttaacaac taattgatcg
     3481 gaatctttta atttttcaat acgagctaaa atttctgaga ggtcgataac ttcaactgaa
     3541 ataccagaat tctccaatat tttttctgaa taacgaaccg tattaaaagc gttaggccga
     3601 gctcctattg ctccagcttt taaattttta aaaccactta cgattctcga tacccctgca
     3661 aaagtagcga tatcttcttt aaaaattgcc gaatcgggat cttcggtatg atcgctggtg
     3721 agggtaaaag gaatattgta ctgccttaaa ttattacaaa t
//
LOCUS       DAOSOL010000125         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24518_length_3757_cov_3.642626, whole genome shotgun sequence.
ACCESSION   DAOSOL010000125 DAOSOL010000000
VERSION     DAOSOL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24518_length_3757_cov_3.642626"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..1045
                     /locus_tag="PK171_04005"
     CDS             44..1045
                     /locus_tag="PK171_04005"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05387.1"
                     /translation="MSIKKILITFSKKIFEEREKNFYSNRLDLHGKTVSMVIEALTQK
                     GWDCHQLNVDVPLAELVPSLSNQNANFVFNLAVCSAEIYDQAFLPSLLDALNIPYLGS
                     NSTIHSFCLDRSLTKLSLRGIGIPTTSSIQWSPGDLLPDSLAYPYIIKSRFRNYCLKL
                     SSDSIVNDQESLLKKAEEVFNQTGEKVLIEKFVEGREMVIGLWGNGNNPEVLPIMELN
                     LSREKPLYDAEIESKKGYVDDISCPAKLSDEHKTMLESMAIKIFRELNLKDFSTFHLI
                     FDQKENLPLFFEINALPLLHYKHSAFPEMCSHRGVDYRSMVQKLLQIAMERIRHERN"
     gene            1032..2138
                     /locus_tag="PK171_04010"
     CDS             1032..2138
                     /locus_tag="PK171_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOT05388.1"
                     /translation="MSAIDATHYAVLGAGHGGQALAGYLSLKGFKVNLYNRTPERLNS
                     IKLMGGIQVEGEIQGFAPLNVVTPNIEEAIKDAEIIMVVVPATGQRFMAENLVPYLKN
                     GQIIVLNPGRTGGALEFRQIFKERNVTAEVIISEAQTFLFASRISGPAQVKIFRIKNS
                     IPVAAIPAYKTVEVVTALRKALPQFVPEDNVLKTSFNNIGAVFHPTLTILNAARIENT
                     HGEFDYYIDGITPSVALILEAVDNERVKVAEALGIRAITAREWLYSAYDAFGRNLYEA
                     IQNNPGYRGIRAPYTIFTRYITEDVPMSLVPISSFGKMLQIPTPTIDCMIQLANILHN
                     RDYFVEGRTIEKLGLAGLSVKEIREMIVVETNSS"
     gene            2156..>3757
                     /locus_tag="PK171_04015"
     CDS             2156..>3757
                     /locus_tag="PK171_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05389.1"
                     /translation="MAEKKTIIGASLENCVHVAGIYRFLSLAEEYQYTTYFLGPAIPI
                     NQLIECIKEKKPQRVIVGYRLSEDSARSLFIELRRQLDNNHLLDQSTYILSCTPALEP
                     IAQSVGVFQYIFSGKESFDEILESIHQTTQDIPPDKKYPSTLPERILAKKPFPLLRHH
                     FGRPSVEETIKGVAQISHSKTIDVISLGPDQNAQEFFFEPEKMKVDESGAGGVPVRSR
                     EDLEKIYAASRVGNYPLLRCYSGTNYLEDWAQLLSETIHIAWGAVPLTWYSRLDGRSQ
                     RPIKTAITENQNAMKKYASIGIPLEVNESHQWSLRDAPDSVACAAFFLAAYNAKKAGA
                     KHYIAQYMFNNPNGISPRADLAKMLAKKELITPLEDKNFTIYTQTRGGLAHFSTNMNI
                     AKGQLGASTILGLVLKPDIIHVVGYSEADHLIYPNELIESAEIVQGIIKDLLFDFPNW
                     EKDQVIQEEKERLINEARILLKAIKNIHHKEDGDDPWVSPATLARAIQKGILDAPHLL
                     GNPEACGQVRTRIVKEVLRVVSKSGE"
BASE COUNT         1146 a          757 c          744 g         1110 t
ORIGIN      
        1 tgaattgaat ttaatactag gtcatattct ctaaaaggag gagatgagta taaaaaaaat
       61 cctcattaca ttcagtaaaa agatatttga agaaagagaa aagaattttt attccaatag
      121 actcgattta catggaaaaa cagtttctat ggttattgaa gctctgacac aaaagggttg
      181 ggactgccat caactcaatg tagatgtccc gcttgcagaa ttagttccct ctctctcaaa
      241 tcaaaatgca aattttgttt ttaatcttgc agtttgctca gcagaaatat acgatcaagc
      301 tttcttaccg tcattattag atgccctgaa tattccctat cttggttcga attcaactat
      361 tcactccttt tgccttgatc ggtctctcac caaactttcc ttgaggggta ttggtattcc
      421 aaccacttca tctatccaat ggtcacctgg ggatttacta cctgatagct tagcttatcc
      481 ttatataata aaatctcgtt ttcgtaatta ttgccttaag ttatcgtctg attcaatagt
      541 gaatgaccag gaaagcctgc taaaaaaggc tgaagaggtt tttaaccaaa ccggtgagaa
      601 agtattgatt gaaaaattcg ttgagggtcg agaaatggtg atcggtctct ggggaaacgg
      661 gaataacccc gaggttctcc ctattatgga actgaatctt tcccgagaaa aaccccttta
      721 tgatgccgaa atcgaaagca aaaaggggta cgttgatgat atatcctgcc cagcaaaatt
      781 aagtgatgag cataaaacca tgttagaaag catggccata aaaattttcc gtgaactaaa
      841 cctcaaagac ttttctacct tccatctcat ttttgatcaa aaagaaaacc tgcctctctt
      901 ttttgaaata aatgctctac cgcttctcca ctataaacat agtgctttcc cggaaatgtg
      961 ttctcatcgc ggtgtagact atcgttcaat ggtacaaaag cttcttcaaa ttgctatgga
     1021 aaggataaga catgagcgca attgatgcaa ctcattatgc agtattggga gccggtcatg
     1081 gcggccaggc tttggctgga tatctttctt taaaaggttt caaagtcaac ttatacaatc
     1141 gaactccaga acgattaaat tctataaaat tgatgggtgg tattcaggtt gaaggagaaa
     1201 tccagggatt tgctccactt aatgttgtta ctccaaacat tgaagaagca atcaaagatg
     1261 ctgaaattat tatggttgtt gttccggcaa ccggtcagcg cttcatggcg gaaaatctcg
     1321 ttccctatct caagaatggt caaattatag ttcttaatcc tggccgtact ggtggagcgt
     1381 tggagtttag gcaaatattt aaagagcgaa atgtgactgc tgaggtgatc atatctgaag
     1441 ctcaaacctt tctttttgcg agtcgaatca gtggtccggc ccaagtaaaa attttccgaa
     1501 ttaaaaatag cataccagta gctgctattc cagcctataa aaccgttgaa gtagttactg
     1561 ctcttcggaa agcacttccc cagtttgttc ctgaagataa tgttctcaaa accagtttta
     1621 ataatattgg agccgttttt catcccactt taaccattct caatgcagct cgcattgaaa
     1681 acacccatgg tgaatttgat tattatatcg atggtattac cccttcagtt gctttaatcc
     1741 tcgaagcagt tgacaacgag cgagttaaag tagctgaggc tttagggata agagccatta
     1801 ctgcacgaga atggctctac tctgcttatg atgcttttgg aagaaacctt tatgaggcta
     1861 tccagaacaa tcctggttac cgcggaatcc gggctcccta taccattttt actcgttata
     1921 ttactgagga tgttcccatg agcctggttc caatctcatc ctttgggaaa atgttacaaa
     1981 tcccaactcc aacgattgat tgtatgatcc agttagccaa cattcttcac aaccgggatt
     2041 actttgttga aggaaggacc atagaaaagc tgggattagc gggacttagt gtaaaagaaa
     2101 tacgggaaat gatcgtggta gaaaccaact caagctaatg atcggaggtt ttgacatggc
     2161 tgagaagaaa acaataattg gtgcttcttt agaaaactgt gttcatgttg ctggaattta
     2221 tcgtttcctt agtttagccg aagaatacca atacacaacc tattttttgg gaccagcaat
     2281 acccatcaac cagttgattg aatgtattaa agaaaaaaaa cctcaacgtg ttattgtggg
     2341 ctaccgctta agtgaagatt cggctcggtc tctctttatc gaattacgcc gccaattaga
     2401 caataatcat cttcttgatc aaagtactta tattcttagt tgtacaccag ctttagaacc
     2461 aattgctcag agtgttggtg tttttcaata tattttcagc ggcaaagaga gttttgatga
     2521 aatccttgaa agtattcatc aaactaccca agatatccct cccgataaaa aatatccctc
     2581 cactcttcct gaaaggattc ttgccaaaaa acccttccct ctccttcgac atcattttgg
     2641 aagaccaagc gttgaagaaa ccattaaagg tgtcgctcaa atcagccatt caaaaaccat
     2701 tgatgttatt tccttgggac cggaccaaaa tgcccaagag ttcttttttg aacctgaaaa
     2761 aatgaaagtt gatgaaagcg gagctggtgg agtcccggtt agaagtcgtg aggacctgga
     2821 aaaaatctat gctgcttctc gggttgggaa ctaccctctg ctgcgttgct atagtggaac
     2881 caactatctg gaagactggg ctcaattatt atctgaaacc attcatattg cttggggtgc
     2941 agtcccctta acctggtaca gccgattgga tggtcgatcc caacgtccaa taaaaaccgc
     3001 tattactgaa aatcaaaatg ccatgaaaaa atatgcttct attggtattc ccttggaagt
     3061 caacgaatcc catcagtgga gcctgagaga tgcgccggat agtgtcgcct gtgctgcttt
     3121 tttcttagca gcttataatg caaaaaaagc cggtgcaaaa cactacatag ctcaatatat
     3181 gttcaataat cccaatggaa tctctcctcg agctgattta gcaaaaatgc ttgccaaaaa
     3241 agaattaatt accccccttg aggataaaaa ttttaccatt tatactcaaa cccggggagg
     3301 gttggctcat ttttctacca atatgaatat tgccaaagga caattagggg catccacaat
     3361 attgggcttg gttctcaaac cggatattat tcacgttgtt ggttatagcg aagctgatca
     3421 tcttatctat cccaatgagc tcattgaaag tgccgagatt gttcagggta tcatcaagga
     3481 cttgctcttt gattttccca actgggaaaa ggatcaagta attcaagaag aaaaagaacg
     3541 tttgatcaat gaagcaagaa ttcttcttaa ggctattaaa aatattcatc ataaggagga
     3601 cggagatgat ccctgggttt ctcccgctac tcttgctcgt gccatccaaa aaggtatttt
     3661 ggatgcaccc catctgttag gaaaccctga ggcttgtggt caagttcgaa ctcgaattgt
     3721 taaagaggtt ttaagagtag ttagcaaaag tggagaa
//
LOCUS       DAOSOL010000126         3741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24653_length_3741_cov_4.511666, whole genome shotgun sequence.
ACCESSION   DAOSOL010000126 DAOSOL010000000
VERSION     DAOSOL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24653_length_3741_cov_4.511666"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2188
                     /locus_tag="PK171_04020"
     CDS             <1..2188
                     /locus_tag="PK171_04020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOT05390.1"
                     /translation="FVASPSTLFFHSTMGVMSLLIAYYILFLGTNYLRLSSQPFIFSI
                     SILSIVYILIDFIRIFLFWRNPFLLRNIIDLTIQLWLIARFAECAIFLIGFLAWFKRY
                     RFLYLGLIYLGLVIFLFCSSFLWNIFPQSYQPGLGLTNFSLVSEYIISAFIYITLSIL
                     LFWKKKISVSVLRPLIISLILSIGAEVVFTMLRNQYGFMIEWRVTVKFLSLLFISQAV
                     VQTIQNRSGKTRLSNYWWSHFKQNSQPQGETDDSKLGQVSFSPDNFNLPNHIQSLVDN
                     AQDLLFRFQAVEPMKLEYINPAVERIIGFTPSEFYADPTLFIQRVFPEDRPLLDLYLK
                     NPTALDKPLAIRMIRKDGTTIWMEHKYVPVYNRRKKTIAIEGIARDITERKIAEEKIR
                     YISFHDPLTGLYNRAFFEEEISRQNFPENHPIGIILGDVNEIHLVNERFGHQEGDRIL
                     IDTANILKRVSRNQDILARWDEDEFILLIPQVDNEYLQFTVESIQKELHNIETNPLQV
                     SISFGYAVKENQDRLITEVIETAEGWMYWNKLTRSNELSQDLVTAIEKSLRKITQETE
                     THSDRLKKITQKIGQELGFSQEELHHLNLLARLHDLGKIATPKSILNKSGLLSPKEWD
                     MVKRHPKIGYQIALNSSELTPIAEAILFHHERWDGTGYPQGLKGNAIPLLSRVVAIAD
                     TYDVLVDGRTYKKPISKKEALNEIKKGAGYQFDPHLVEVFLRVMSQ"
     gene            complement(2234..3382)
                     /locus_tag="PK171_04025"
     CDS             complement(2234..3382)
                     /locus_tag="PK171_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT05391.1"
                     /translation="MNHSLKVGVIGTGFIGPAHIEAARRTFLADVISLADVNDEIAQN
                     KALQYNVKNHFGNYRDLLKDDKIQIVHICTPNHLHFQMSKEALEAGKHVICEKPLAMN
                     RQEAEELVELADKKGLINAVHFNIRYYPLARESQRKVKKGELGEIFAVHGSYLQDWLF
                     YPTDYNWRLESSMSGESRAVADVGSHWLDMVEFVTGEKIIQVLADFATFYPIRKKPLK
                     PVETYAGKILHPEDYQDIAINTEDYATVLLRFGNGGHGVLTVNQVAAGRKNRLYFEID
                     GSQKAIAWDSEKPNELWIGYRDQANQTLLKDPSLLDSETRKIVSFPGGHNEGFPDTSK
                     QLFKEVYQAVLDPSAPKNYPGFKDGLREVLLCEAILKSSKNNQWVEVK"
BASE COUNT         1115 a          722 c          686 g         1218 t
ORIGIN      
        1 ctttgtagct tcaccatcaa cgctgttctt tcatagtacc atgggggtta tgagcctcct
       61 aattgcttat tatatcttat ttcttggcac gaattattta cggctatctt cccaaccctt
      121 catttttagt atcagcattt tatcgattgt ttatattctg attgatttta tacgaatttt
      181 tcttttttgg agaaatccct ttttattgag aaatattatt gacttgacca tccagctctg
      241 gttgattgct cgttttgctg agtgtgcaat ttttttaatc ggctttcttg cttggtttaa
      301 aagataccgt tttttatatt taggacttat ttatttaggc ttagtaatat ttttattttg
      361 tagttcattt ctatggaaca tatttcctca aagttatcaa cccggcttag gattaaccaa
      421 tttttcttta gtgagtgaat atatcatcag cgcttttatt tatataacct tgtctattct
      481 tcttttttgg aaaaagaaaa tttccgtttc agtacttcgt ccgctcatta tttccttgat
      541 tcttagtatt ggagcggaag tggtattcac catgcttcgt aaccaatatg gatttatgat
      601 cgaatggcgg gttactgtca agtttttatc tcttctcttt atttctcagg cagttgtcca
      661 gactattcaa aatagaagtg gaaagacccg tttatcgaat tattggtgga gtcattttaa
      721 gcaaaattcc caaccccagg gagaaaccga cgactctaaa ttaggccagg tctctttttc
      781 tcccgataat tttaatcttc ctaatcatat ccaatcttta gttgataatg ctcaggacct
      841 tctcttccgt ttccaggcgg ttgaacccat gaagctggag tatattaatc cagcagtaga
      901 aagaataatc ggattcaccc catctgaatt ttatgccgac cctactcttt ttattcaaag
      961 agtatttcct gaggatcgac cacttttgga tttatattta aaaaatccca cagctcttga
     1021 taaacctctt gccatccgaa tgattcgtaa agatggcacc actatctgga tggaacataa
     1081 atatgtgccg gtttataacc gcagaaaaaa aactatagct atagagggaa ttgctcgaga
     1141 tattactgag agaaagatag ccgaagaaaa gattcgatat atcagtttcc atgacccact
     1201 taccgggtta tacaatcgag ctttttttga agaagagatt tcccgtcaaa attttcctga
     1261 aaaccaccca atcggtatta ttttaggaga tgtaaatgaa attcacttag taaatgaaag
     1321 gtttggtcat caggagggtg atcgaattct tattgatacc gctaatatct tgaagagggt
     1381 ttctcgaaat caagatattt tagcccgctg ggatgaagat gaattcatac ttttaatacc
     1441 tcaagtcgac aatgaatatc tccagtttac cgtagaatca atacaaaaag aattacataa
     1501 tatcgaaacc aatcctctcc aagtcagtat ttcctttgga tatgctgtga aagaaaatca
     1561 ggatcgattg ataactgaag ttattgaaac agctgaagga tggatgtatt ggaacaaatt
     1621 gacgagaagt aatgaattaa gtcaagatct ggttaccgct atagaaaagt cgcttcgtaa
     1681 aattactcaa gaaaccgaaa ctcattccga tcgactcaaa aaaattaccc aaaaaattgg
     1741 ccaagaatta gggttttccc aagaagaact ccatcacttg aaccttttag ctcggctgca
     1801 cgatttggga aaaattgcta ctcccaaatc aatattaaat aaatctgggc ttctttctcc
     1861 gaaagaatgg gatatggtaa aacgacatcc caagattggc tatcagatcg ccttgaattc
     1921 ttcagaatta accccgattg cagaagctat tctttttcat cacgaaagat gggatgggac
     1981 tggttatccc caaggattaa aggggaatgc tattcctctc ctatcaaggg ttgtagcaat
     2041 tgccgatacc tatgatgttt tggttgatgg aagaacctat aagaaaccga tatccaaaaa
     2101 agaagctttg aatgagataa aaaaaggtgc aggttatcag ttcgacccgc acctggttga
     2161 agtatttcta agagtaatga gtcaataggt attatataat ttcgaatcgc tcattgtttc
     2221 ttgtcactgt cttttatttt acttctaccc attgattgtt tttggagctt tttagaattg
     2281 cttcacacaa aagtacttct cgaaggccat ctttaaaacc cggataattt ttcggagcgg
     2341 atggatccaa aacagcttga tacacttctt tgaagagttg tttcgaggta tcagggaaac
     2401 cttcgttgtg ccctccaggg aaagaaacaa tttttctggt ttcactgtct aagagcgaag
     2461 gatcttttaa gagcgtttga ttagcttgat ctcgataacc aatccagagt tcgttgggtt
     2521 tttccgaatc ccaggcaatt gctttttgag agccatctat ttcaaagtac aagcgatttt
     2581 ttcttccagc tgcgacctga ttcacggtca acacaccatg acctccattc ccaaaacgga
     2641 gaagaacagt ggcgtaatct tcagtgttaa ttgcaatgtc ttggtaatct tccggatgaa
     2701 gaatttttcc tgcataggtt tcaactggtt tcaagggttt tttccgaatg ggatagaaag
     2761 tagcaaagtc agctaatact tggatgattt tttcaccagt aacgaattca accatatcca
     2821 accaatgaga accgacatca gcaaccgcac gggattcgcc actcatgctg gattccaatc
     2881 tccaattata atcagtagga tagaatagcc aatcttgtaa ataagacccg tgaacagcaa
     2941 aaatttcccc aagctcacct ttcttaacct tgcgttgtga ttctcgagct aagggataat
     3001 aacgaatatt gaagtggaca gcattgatta aacctttttt atcagctaat tcaaccagct
     3061 cttctgcttc ctggcggttc atagccaaag gcttttcaca aataacatgc tttccagcct
     3121 ctaatgcttc tttgctcatt tgaaaatgaa gatggttggg agtacagatg tgtactattt
     3181 gaattttatc gtcttttagt aaatcgcggt aatttccaaa atggtttttc acgttatact
     3241 ggagagcttt attctgggca atttcatcgt taacatcagc aaggctaatg acatcagcta
     3301 aaaaagttcg ccgtgctgcc tcgatatggg caggaccaat aaagccagtt ccaatcacac
     3361 caactttaag tgaatgattc atatcgcttc ctcctttata gatattatga tttataaaga
     3421 tattgaaaaa agactatcct gaaaatacag gaacgagtaa aaaatgttgt catcctgagc
     3481 cctcactttt ttaggagcgt gaggcgggaa acggttcggg attggttttt ttccctgttc
     3541 tttcaaatcc cccctacccc cttttctaaa gggagagatt gatccttatc ctaaaaggta
     3601 aatacaatac caataattaa tttaattaga atttatgggg gtttgatcaa tcaagcccct
     3661 accaaagaat ataaaattgt aggagtacaa tgtattgtgc ccattcttta attaatgtag
     3721 cgacctgcca tggcatgtcg a
//
LOCUS       DAOSOL010000127         3734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24709_length_3734_cov_4.365588, whole genome shotgun sequence.
ACCESSION   DAOSOL010000127 DAOSOL010000000
VERSION     DAOSOL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24709_length_3734_cov_4.365588"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1172
                     /gene="hydE"
                     /locus_tag="PK171_04030"
     CDS             78..1172
                     /gene="hydE"
                     /locus_tag="PK171_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOT05392.1"
                     /translation="MREVVNDQPMLVNERRGFNQENLRQLFDLAEHNLPSLLKYANQI
                     RKEQVGDEVHLRGIIEFSNYCSCHCLYCGLRAENRKISRYRLTNQEIFQSVQTAVNYG
                     LKTIVLQSGEDRFYQPEDIARLIEFIKKHFDVAITLSVGEHPHQSYQIWRNAGADRYL
                     LKHETADPILYQKLRPGKNLQDRIECLLDLKDLGYQIGSGNMVGLPGQTLTSLIDDVL
                     LMKKLNVEMAGIGPFLPHPDTPLVGSSAGSLTETLKILAITRIILPTIHLPATTAIST
                     VNDDARRLALESGANVIMPNFTPLTVRDQYVIYPQKIDIIEDPEQSVNKLKKLLKEMN
                     RTISMSYGHAVKIKTYSLTKKEAYHGFGSQ"
     gene            1153..2586
                     /gene="hydG"
                     /locus_tag="PK171_04035"
     CDS             1153..2586
                     /gene="hydG"
                     /locus_tag="PK171_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOT05393.1"
                     /translation="MVLVLNERKKADFIDENSIENLLEKGEYRHRKEIMDIIEKARMA
                     QGLSPEECAVLLQVEDPEILTEIYKVAQEIKLKIYGKRLVFFAPLYISNYCINNCRYC
                     GYRQDNDFHRRRLSQEEVIEEVKILESMGHKRLALEAGEDLVNCPLDYILETIHTIYG
                     VNKGKGSIRRVNVNIAATTVNNYRKLKEAKIGTYILFQETYHRETYQYMHPQGPKNDY
                     DYHTTALDRAMEAGIDDVGAGVLFGLYNYKFEVLSLLQHALHLEEVFGVGPHTISVPR
                     LRPALGVDSLLSQHLVNDDEFRKIIAVLRLSVPYTGIILSTREKASFRDELASLGISQ
                     LSAGSCTGVGGYREEYDQIKESTGIIQDSPQFQVEDHRSPDEVLRSVCQSGYIPSFCT
                     ACYRKGRTGDRFMSLAKSGQIQNVCQPNAILTFKEFLEDYASEETKKIGKKTIKEHLE
                     AIPNLQVRKLTEERLKLIENGERDLYF"
     gene            2816..>3734
                     /gene="hydF"
                     /locus_tag="PK171_04040"
     CDS             2816..>3734
                     /gene="hydF"
                     /locus_tag="PK171_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOT05394.1"
                     /translation="MNGTPRSLRPHIGIFGRRNVGKSSLINALTNQEVAIVSSEPGTT
                     TDPVFKSMELLPFGPVVFIDTAGLDDQGFLGELRKKKSLEILRRCDVAILVWDNQGGD
                     FFFEKELIALLKENKIYTIGVQNKTNEKILQNTELDQFQIPLFETDAQTGKGIDILKN
                     SLVEILKRQYEEKPLIKDLIQPRDVVLLVVPIDIGAPKGRLIIPQIQTIREILDTGAS
                     AIITKESELAEILNKQKYPPGLVVTDSQVFHNVAAVLPPDQPLTSFSILFARNKGDLT
                     TLVEGVQMIDTLKPTDKVLIAEACTHHPQT"
BASE COUNT         1139 a          778 c          765 g         1052 t
ORIGIN      
        1 ctttgaccgg ccgtttggga caaattcccg gtatcaaagt taaaagcacc ttagtcactc
       61 gataaaagag agaaggagtg agagaagtag tgaacgacca acccatgctg gtcaatgaac
      121 ggaggggttt taatcaagaa aaccttcgac aattgtttga tttagctgag cacaatcttc
      181 cttccctctt aaagtatgcc aaccagatcc gtaaagaaca ggttggagat gaagttcacc
      241 tccgggggat tattgagttt tctaattact gttcctgtca ttgtctctac tgtggattac
      301 gggcagaaaa ccgcaaaata tccaggtatc gcttaaccaa tcaggaaatt tttcaatcag
      361 ttcagactgc agtcaattac ggattgaaaa ctatcgtcct tcaatcagga gaagaccggt
      421 tttatcaacc tgaggatatt gcgcggctca tcgaattcat taaaaagcat tttgacgttg
      481 cgattacctt atccgtagga gaacaccccc atcagagtta tcaaatctgg cggaatgccg
      541 gagctgatcg ctatttatta aaacacgaaa ccgctgaccc tattctctac cagaaattaa
      601 gaccagggaa aaaccttcaa gatcgaatcg aatgcttgtt ggatttaaaa gacctcggtt
      661 accaaattgg ttcgggaaat atggttgggc tccccggtca aactttaaca tcgctaattg
      721 atgatgttct tctgatgaaa aaattaaatg ttgaaatggc tggtattggc ccctttcttc
      781 cccatcccga cacgccttta gttggttctt ctgccggttc tctcacggaa accttaaaaa
      841 ttctggctat tacccgaatc attcttccga ccatccatct tccagccacc actgccatca
      901 gtacagtaaa tgacgatgct cgccgtttag ccttagaatc gggagcaaat gtcatcatgc
      961 ccaacttcac gcctttaaca gttcgcgacc aatatgtcat ttatcctcaa aaaatcgaca
     1021 ttatcgaaga ccccgaacaa agtgtgaaca agctaaaaaa actcttgaaa gaaatgaatc
     1081 ggaccatttc gatgagttat ggtcacgcgg taaaaataaa aacatattct ttaaccaaaa
     1141 aggaggctta tcatggtttt ggttctcaat gaaagaaaaa aagctgattt tatcgatgaa
     1201 aatagtattg agaatttatt agaaaaggga gaatatcgtc atcgaaaaga aattatggat
     1261 atcatcgaga aagcccgaat ggcccaaggt ctttctcctg aagaatgtgc cgttctcctt
     1321 caggttgaag accctgaaat attaaccgag atttataaag ttgctcagga gattaaatta
     1381 aaaatttacg gaaaaagact ggtttttttt gctcctcttt acataagcaa ttactgtatt
     1441 aataattgcc gatattgtgg gtatcggcaa gataacgatt ttcatcgtcg tcgcttaagt
     1501 caagaagaag tcattgaaga agtgaagatc cttgagtcca tgggccacaa aagattagcc
     1561 ttagaagccg gagaagatct ggtgaactgt cctttggatt acatacttga aaccattcat
     1621 actatatatg gagtcaacaa gggaaaagga agcattcgga gggtgaacgt caatattgcc
     1681 gctaccaccg tcaataacta ccgaaaattg aaagaagcca aaattggaac ttacattctt
     1741 ttccaggaaa cctaccatcg tgaaacctat caatacatgc atccacaggg accaaaaaat
     1801 gattatgatt atcacaccac tgctttggac cgggctatgg aagccggcat cgacgatgtc
     1861 ggtgctgggg ttttgtttgg cctctacaat tataaatttg aagttctcag cttgctacag
     1921 catgctcttc atttggaaga agtatttgga gttggcccgc acactatttc ggtacctcgt
     1981 ttgcggcctg ctttgggagt tgactcactc ctctctcaac acctagtcaa tgacgatgaa
     2041 tttcgtaaaa tcatcgccgt gctccgtcta tcagttccct ataccggaat catcctttcc
     2101 acccgggaaa aagcctcttt tcgagatgag ttagcttcgt tggggatttc tcagcttagt
     2161 gctggttctt gtaccggtgt tggtggatat cgggaagaat atgatcaaat aaaggaaagt
     2221 actggaataa ttcaggattc tccacaattt caagtggaag accaccgcag ccccgatgaa
     2281 gtcttgagga gtgtatgcca atcgggttat attcccagct tttgtaccgc ttgctaccgt
     2341 aaagggcgaa ctggagatcg tttcatgtct ttagcaaaat ccggacaaat tcagaatgtg
     2401 tgtcagccta atgccatcct tacttttaag gaatttttag aagattatgc ttcggaggaa
     2461 actaaaaaaa taggcaaaaa aactatcaag gaacatcttg aagccattcc taatttacag
     2521 gtgagaaaac taactgagga acgcctaaaa ctcatcgaaa atggcgaacg agatttatat
     2581 ttttaaccgg agacggtgtt atttttttta taattttctt ctgatcattt ttactttttc
     2641 ccttaatggg agatgatgag gatgagggcg aaagcccaaa attcgacatg ccatgacagg
     2701 tcgctacatt aatcgggcac aatacattat gcctctacaa ttttattttt tttgtagggg
     2761 cttgatttat catgcccgct ggttttcggg ataaaaataa ttggaggtta cagtcatgaa
     2821 cggaacccca cgaagccttc gaccccatat tggtattttt ggccggagaa atgtcggcaa
     2881 atcatcactg atcaacgctc tgactaatca agaagttgcc attgtttcca gtgagccagg
     2941 aaccactacc gatccggttt tcaaatctat ggaactcttg ccttttggac cagtagtttt
     3001 tatcgatact gctggattag atgaccaagg gtttttaggt gaattaagaa aaaagaaatc
     3061 attggaaatc cttcgccgtt gtgatgtcgc tatattggtt tgggataacc aggggggaga
     3121 ttttttcttt gaaaaagaac tgatcgcttt gctcaaagaa aacaaaattt acaccattgg
     3181 ggttcaaaac aaaaccaatg aaaaaattct tcagaataca gaactcgatc agttccagat
     3241 ccctcttttt gaaaccgatg ctcaaaccgg aaaaggcatc gacatattaa aaaattcatt
     3301 ggttgaaatt ctaaaacgtc agtatgaaga aaaacctctg atcaaagatt taattcagcc
     3361 aagagacgta gtgcttttgg ttgtcccgat tgatatcggt gctcccaagg ggcggttgat
     3421 catcccccag atccaaacca tacgtgaaat attagatacc ggagcttcag caattattac
     3481 caaagaaagt gagttagctg aaattttgaa taagcaaaaa tatccccctg gtttggtggt
     3541 taccgattct caggtttttc acaatgttgc cgcagtcctc cctcctgatc aacctttgac
     3601 cagtttttcg attttattcg ctcgaaacaa aggagattta accactctgg ttgagggagt
     3661 tcagatgatt gacactctca agccgacgga caaagttctc attgccgaag cctgcaccca
     3721 ccatcctcaa accg
//
LOCUS       DAOSOL010000128         3733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24718_length_3733_cov_4.513322, whole genome shotgun sequence.
ACCESSION   DAOSOL010000128 DAOSOL010000000
VERSION     DAOSOL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24718_length_3733_cov_4.513322"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..836)
                     /locus_tag="PK171_04045"
     CDS             complement(6..836)
                     /locus_tag="PK171_04045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT05395.1"
                     /translation="MVLLLLLLIGVLSSEQFAYSQDAETFYKNGYIYFSQNNFEKAKE
                     SYQQAIQIKPDYWDARYWLGKTYEMMEEFPQAMNEWKIILQNQPGHWEAFKKWRSYVA
                     SQVRISNQVRQNLENVFLYQNGSPENYRDQPWDTIIPYGLAIARQNDFISAFLSARLF
                     RWIGSQVSGLLSDQADISYQKALDLAAISPPEDSELVFQLIKDVTNYYGQNNALQKKV
                     ETLYQSILAQQAGVTVEESVNTSQIEIKVTASGVESGPADNRNQDGSNPRFYIDTDIE
                     "
     gene            complement(873..>3733)
                     /locus_tag="PK171_04050"
     CDS             complement(873..>3733)
                     /locus_tag="PK171_04050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT05396.1"
                     /translation="QIGKKLNIPFVATNDSHYLNQEDARVHEIILAIQTATNLNDPKR
                     LRFPTQEFFLKTPEEMIRDFSSVPEAIENTQRIAESCEVKLELGQVLLPEFSVPEGFD
                     VPGYLRHLCLEGLQHRYGDPPPEKVRNQLEYELTVINNMGYDAYFLIVWDFVKYSREK
                     GIMVGPGRGSAAGSLVSYSLGITNIDPLRYGLLFERFLNPERISMPDIDIDFCFERRG
                     EVIDYVSNKYGHTHVAQIITFGRMMARGAVRDVGRALGWSYGEVDKIAKLIPGAPGVT
                     LERAIDTNTELKKLVKNDSNVAQLLEIAQRIEGLCRHASMHAAGVVISHKPLTEYVPL
                     QKMSNNEIVTQFDMDTLGELGLLKMDFLGLRTLTVIERTLNIIKKTAQKEINLDTIAL
                     DDQETYELLGRGETTGVFQLESSGMKELLKRFHPTVIEELTALLALYRPGPLGSGMID
                     DFIKRKHGKVKVTYPHPSLEEILKETYGVILYQEQVMKIASELAGFTLGQADILRRAM
                     GKKKADVMEQQRDRFIQGAKEKGHDPKTAAEIFDLIEYFAGYGFNKSHSAAYAFISYQ
                     TAYLKAHYPTEYLTACLTSIQEDTDKIAKFIMEARRLSIKILPPDVNESLANFTVVGE
                     KKIRFGLAAIKNVGENAVNEILTRRKEARFQNIFDFMERVDQRVINKRVVESLIKAGA
                     FDSLHHNRNQLFSSLEEIIHFYAKRKKRNQPQQATLFGDLKPIMQETLTLKEVSEFRT
                     RDLLAMEKEIIGLYISDHPVRNALASYTFLNVIPFEQVQMMKEKAAVRIMGAIVESRR
                     ITTKTGKDMYFVTLEDETGTLETIFFPKIAEKLQNILEKENVLCLEGRVDVLDSGVNK
                     VIVEKILDLEKIKQNERPVHIEISNPDDPLSVFYSLKDCLQRHNGTQPVILHMIGNQE
                     RWAIEMGEQFRVSWNQELEQALFNILEGVQKKRVWLAG"
BASE COUNT         1029 a          783 c          735 g         1186 t
ORIGIN      
        1 cggaatcatt caatgtcggt atctatataa aatcttggat tagaaccatc ttgattccgg
       61 ttgtctgcag gcccactttc aacaccactt gccgtcactt tgatttctat ttgagacgta
      121 ttgacggatt cttcaactgt tactccagct tgttgggcta agatgctttg atacaaggtt
      181 tctacttttt tctgcagggc attgttttga ccataatagt tcgtaacatc tttgatcagc
      241 tgaaagacta gctccgaatc ttcgggtgga ctgatagcgg ctaaatctaa ggctttttgg
      301 taactgatat cagcttggtc agaaagtaaa ccactgacct gagatcctat ccaacggaat
      361 aaacgagcag ataaaaaagc tgagataaag tcgttttgtc tggcaattgc taacccatag
      421 gggatgatag tatcccatgg ttgatcccga taattttccg gagaaccatt ctgatagaga
      481 aaaacatttt ctaagttttg ccttacttgg ttgctgattc gaacctgaga tgccacatag
      541 gatcgccatt ttttaaatgc ttcccaatga ccgggttgat tttggagaat tatcttccac
      601 tcattcattg cctggggaaa ttcttccatc atctcatagg tttttcccaa ccagtaacga
      661 gcatcccaat aatcaggttt gatttgaata gcctgttgat aggattcttt cgctttctca
      721 aagttgtttt gggagaaata aatatagcca tttttataaa aagtttctgc atcttgagaa
      781 taagcaaatt gctccgaaga aagaacaccg atgagcaaca aaagcaacag taccaagcaa
      841 aaccatctcc gataggtcat caccatatca cctcatccag ccaaccaaac tctctttttt
      901 tgaacacctt ccaaaatatt aaacagggct tgttcgagtt cttgattcca actcactcga
      961 aattgttcac ccatttcaat cgcccatcgt tcctggttac caatcatatg cagaatcact
     1021 ggttgtgtcc cattatgtct ttgaagacaa tcttttaagc tatagaaaac cgataaagga
     1081 tcatcgggat tggatatttc tatgtgtact ggtctttcat tttgttttat tttttccaga
     1141 tctaatattt tttcaacaat gactttatta accccggaat ccaatacatc tactcttccc
     1201 tctaaacaga gaacattctc tttttcaaga atattttgaa gcttttcggc aatttttggg
     1261 aaaaagatgg tttctaatgt tccggtttca tcttcaaggg ttacaaagta catatctttt
     1321 ccagttttgg tggtgattct tctggattca acgatggctc ccataatccg aaccgcagct
     1381 ttttccttca tcatttgaac ttgttcaaag ggaatgacat ttaaaaatgt gtaggaggca
     1441 agagcatttc taactggatg atcactgata taaaggccga ttatttcttt ttccatggcg
     1501 agcaggtctc gagttcgaaa ttctgagact tccttgaggg taagtgtttc ttgcatgatc
     1561 ggtttcaaat cgccaaaaag tgtagcctgt tgaggttgat ttcttttttt tcgtttggca
     1621 taaaaatgga taatttcttc taaggagctg aagagttgat ttcgattgtg atgaagactg
     1681 tcgaaagctc cggctttgat gaggctttcg acaacccgtt tgttaattac tctttgatcg
     1741 acccgttcca taaagtcgaa tatattttga aaacgagctt cttttctccg agttaaaatt
     1801 tcgttcacgg cgttttcgcc aacattctta atcgctgcca gtccaaatcg gattttcttt
     1861 tccccaacta cggtaaaatt ggctaaactc tcgttgacat caggtggaag tattttgatg
     1921 gatagtcggc gagcttccat aataaattta gcaattttat cagtatcttc ctgaatactg
     1981 gtaaggcaag cggtgagata ctcggtggga taatgggctt tgagataggc agtttgatag
     2041 gatataaaag cataagcggc actatgtgac ttgttaaaac catatccagc aaaatattct
     2101 ataaggtcaa aaatctcggc agcggttttg gggtcgtgac ctttttcttt ggctccctgg
     2161 ataaaccggt ctctttgttg ctccataacg tcggcttttt tctttcccat ggctcgacgg
     2221 agaatatcgg cctgacccag ggtaaaacct gctagttcac tggcaatttt catgacctgc
     2281 tcctggtaga gaatgacacc ataggtttct ttcaagatct cttccaaact tgggtgagga
     2341 taagttactt taaccttccc atgttttctt ttaatgaaat catcaatcat tccacttccc
     2401 aatggcccag gtcgatagag ggctaaaagc gccgtcaatt cttcaatcac agtggggtgg
     2461 aatcttttta gaagttcctt cataccagag ctttccagtt ggaatactcc ggttgtttct
     2521 ccccgcccta ataattcata ggtttcttgg tcatcaagag caatggtatc cagattaatt
     2581 tctttttgag cggttttttt aattatattt aaagttcgtt caatgacggt aagggttctt
     2641 aaacctaaaa aatccatctt gagcaaaccc aactcaccta aagtgtccat atcgaattgg
     2701 gtaacgattt cattgttgct cattttttgg agaggaacat actcggtaag gggtttatgg
     2761 gaaattacta ctccggcagc atgcatcgaa gcgtggcgac agagcccttc aattctttgg
     2821 gcgatttcta aaagttgggc aacgtttgaa tcatttttaa ccagtttttt taattcagtg
     2881 ttggtatcga ttgccctttc aagagtcaca cccggcgctc ctggaatgag cttggcgatt
     2941 ttatcaacct ctccatagct ccagcccaaa gcccgaccca catcacgaac cgcccctcga
     3001 gccatcatgc ggccaaaggt aatgatttga gcaacatggg tgtgtccata tttattggaa
     3061 acgtagtcaa tgacctctcc ccttctttca aagcaaaagt cgatatcaat atcaggcata
     3121 ctaattcgtt ctggattcag aaaacgttcg aataaaagcc cataacgaag gggatcaata
     3181 ttggttattc caagagaata ggaaactaaa ctaccagccg ctgatcctct tcccggtcca
     3241 accatgattc ccttctctcg agaatatttg acaaagtccc atacaatcaa aaaataagca
     3301 tcataaccca tattattaat aacggttaat tcatattcta attggtttct gactttttca
     3361 gggggtggat ctccataacg atgttgaagc ccctcaaggc aaagatgtcg gagatatcca
     3421 ggaacatcaa atccctcagg aacggaaaat tccggtagga gaacctggcc caactccaat
     3481 tttacctcac acgattcggc aatcctttgg gtattctcaa tcgcttcagg aactgaggaa
     3541 aagtcccgga tcatttcttc cggagtttta agaaagaact cttgagtggg gaaacggagt
     3601 ctttttgggt cattgagatt ggtagcggtt tgaatagcca aaataatctc atggactcgg
     3661 gcgtcctcct ggttgagata atgagaatcg ttggttgcta caaatgggat attgagtttt
     3721 tttccaattt gaa
//
LOCUS       DAOSOL010000129         3713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24875_length_3713_cov_3.982231, whole genome shotgun sequence.
ACCESSION   DAOSOL010000129 DAOSOL010000000
VERSION     DAOSOL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24875_length_3713_cov_3.982231"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1897
                     /locus_tag="PK171_04055"
     CDS             194..1897
                     /locus_tag="PK171_04055"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HOT05397.1"
                     /translation="MIKGKGFRLKFIGYLFMILILFFSASGFAQDNYKIENILSSMSL
                     EEKVGQVIFGFFRGPVLTPELEEMIRDYHLGGIILYSSTGNTESPIQVASLINAVQYC
                     AEKHRQIPPLVSIDQEGGLVARLTEGVTVFPGNMALGATRNTEFAQKAAEICARELRI
                     LGITLNFAPVVDVNSNPANPIIGIRSFGSDPEIVSNLGSVMIEPYKKERVLCTAKHFP
                     GHGDAGIDSHTGLPFINRSREDLEKVELVPFQQMVQNGVPAVMSAHIVVPALSEGDDF
                     PATLSKEILSILRNDWGFNGLIITDSMGMGAIDQQWGMEEASILAFQAGADVLLFGSD
                     KNHEPAEQKAVYQALMEAVQTGRISESRLNESVKRILGTKTDAAVFSNPYPNFAMIDE
                     LASPESLKVAREIANQSITLIKNSNHLIPIDQRFSIPIIWPKEYEKSLQILLKNCSNL
                     KPFLLSIESSDEERNALQEKIQDNEFKIVASYDLHRNAGWKELVNAISNQKTIIIALR
                     SPYDFLKVTDYGAAVATYGDRPVSIEALGKILKGEIQPNGQLPVELPGRYQLGWGLKE
                     F"
     gene            1929..2909
                     /locus_tag="PK171_04060"
     CDS             1929..2909
                     /locus_tag="PK171_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOT05398.1"
                     /translation="MKVLVAGIHFESIIHFLRKNLPEYESYQIPASKVVEEAKDASVI
                     IPTMTRIDRHVINSAPHLKLIQQWGSGLEGVDIQAATENGIAVANVPTHGTGNAESVA
                     EWCVMSAIALSRSFPSIQESVWKGFPWGAPCGNSLMGKTAGIVGVGGIGVELAKRLIS
                     FRMKLIGVKKNPHYLPSGLNFEWVKGMNAFPELLSLADYIFLCLPQNEDTRCLFNRKA
                     FQMIKNDAYLINPARGGLIDREALSDALGDERLAGVAMDVFWQEPTSPQDPLLQHPKV
                     LVTPHIAGATDTSYQGIAEGVVKNIRLALSGKIPNNCVNSEVLSQLKWKT"
     gene            complement(2989..3453)
                     /gene="mscL"
                     /locus_tag="PK171_04065"
     CDS             complement(2989..3453)
                     /gene="mscL"
                     /locus_tag="PK171_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOT05399.1"
                     /translation="MSKLIDEFKEFISRGNVVDLAVGFIIGTAFGAIVKSLVDDVIMP
                     PIGLALGKVDFSNLFAVLKEGDTPPPYHTVAAAKEAGAVTLNYGLFINSIITFLIIAL
                     AVFLLVKIINQMQRKKEVPPAAPTTKDCPFCFTAIPIKAVRCPNCTSELSKA"
BASE COUNT         1183 a          677 c          771 g         1082 t
ORIGIN      
        1 acataattca ccattatacc cgaaaagtga attatttaaa tatgaaaaga gtaattcatt
       61 tttcacattt taattcaact caatcatttc tttatcatct aaaaaatctc atgattttac
      121 ttatgaatta tcaaatttct aattatgaat atgatatgat gaaaaatagc aggaaaaagg
      181 aaggagtgat gagatgatta aagggaaagg atttcggtta aagtttattg gatacctatt
      241 catgatattg attcttttct tcagcgcctc tggatttgct caagataatt ataaaatcga
      301 gaacattctt agcagtatgt cacttgagga gaaggttgga caagttatct ttggattttt
      361 tcgaggtcca gttttgactc cagaattgga agaaatgatt cgagattatc atttaggggg
      421 gattattctc tattccagta cagggaacac agaaagtcca attcaagtcg cctctttgat
      481 aaatgccgtt caatattgtg cggaaaaaca tagacaaata cctcccttgg tttcaataga
      541 ccaagaagga ggattggttg ctcgactaac tgaaggggta acagtatttc caggaaatat
      601 ggctttagga gctactagga atactgaatt tgcccagaaa gcagcagaga tttgtgctcg
      661 agaattacgt attcttggga ttactttaaa ttttgcacca gtcgttgacg taaacagtaa
      721 tccagcaaac ccaataatcg gaattcgctc ctttggctct gacccggaaa ttgtttcaaa
      781 tcttggatca gtaatgatag aaccctacaa aaaagagcga gttctctgta cagcaaaaca
      841 ttttcccggt cacggagatg ctggaatcga ttcccacacc ggcttacctt ttatcaatcg
      901 aagccgagaa gatttggaaa aagttgaatt ggttcccttt caacaaatgg tacaaaatgg
      961 tgtaccagca gttatgtcgg ctcatattgt tgtaccggcc ttgagtgaag gagacgattt
     1021 ccctgcgact ctatcgaaag aaatattatc aattctccgt aatgattggg gctttaatgg
     1081 tcttattatt actgattcga tgggaatggg agcgatagac cagcaatggg gtatggaaga
     1141 agcatcgata ctcgcttttc aagcgggagc tgatgtgctt ttatttggat cggataagaa
     1201 tcatgaacca gctgaacaga aagcagtata ccaagctctc atggaggcag ttcaaaccgg
     1261 acgtattagc gaaagtcgat tgaacgaatc ggttaagaga atcttaggta ctaaaactga
     1321 tgcagcagtt ttttccaatc cttatccgaa tttcgcgatg atagatgaat tagcttcacc
     1381 ggagagtctt aaagtagcac gagaaattgc caatcagagt ataactttaa taaaaaattc
     1441 gaatcatctc attccgattg accagcgttt ttctatacca ataatatggc cgaaagagta
     1501 tgaaaaatct ttacagattt tattaaaaaa ctgctccaat ttgaaaccat tccttctctc
     1561 gattgaatct tccgacgagg aaagaaatgc tcttcaagaa aaaattcaag ataatgaatt
     1621 taagatagtt gcttcatatg atcttcatcg aaatgctgga tggaaagaac tggttaatgc
     1681 gatatccaac caaaaaacca tcattattgc cctgcgttct ccttatgatt ttcttaaggt
     1741 gactgattat ggggcggctg ttgcaactta tggagatcgg ccggttagca ttgaagcctt
     1801 aggaaaaatt cttaagggcg aaatacaacc aaatggccaa ctcccagttg aacttccagg
     1861 tcggtatcag ttgggttggg gtttgaaaga attttaaaaa aacaaaaaat gagaaagtga
     1921 ttaacgctat gaaagttttg gttgcaggca tccattttga atcgattata cattttttga
     1981 gaaaaaattt accggaatat gaatcctatc aaatacccgc ttcgaaagtc gttgaggaag
     2041 caaaagatgc gtcggttata ataccaacta tgactcgaat agatcgtcac gtaattaata
     2101 gcgcacctca tctcaaactc atccaacaat ggggttctgg tttggagggt gttgacattc
     2161 aagctgccac tgaaaacggt attgctgtag ccaatgttcc aacccacggt actggaaatg
     2221 ccgagtcggt tgctgaatgg tgtgtcatgt cagccatagc tttaagccgt tcattcccat
     2281 caattcaaga atcggtttgg aagggttttc catggggagc tccttgtggg aatagcctta
     2341 tgggaaaaac cgccggaatt gttggggttg gaggaatagg ggtggaatta gctaaaagac
     2401 tgatttcctt tcgaatgaaa ttgattggtg tgaaaaaaaa tccccattat cttccttcag
     2461 gtttgaattt tgaatgggtt aagggtatga atgcctttcc tgagctatta agcctagctg
     2521 attatatttt tttgtgcttg ccgcaaaatg aagatactcg atgccttttt aatcgtaagg
     2581 catttcaaat gatcaagaat gatgcttatc taattaatcc agctcgaggc ggattgattg
     2641 atcgagaagc tctttccgat gcactggggg atgaacgatt agcaggcgtg gcgatggacg
     2701 tattttggca ggaacccact agtcctcaag atccactcct acaacaccca aaagttttag
     2761 ttactcctca tatagcagga gcaaccgata cttcctatca ggggatagcc gaaggggttg
     2821 tgaaaaatat tcggttagca ctttcaggga aaataccaaa taattgtgtt aactcagaag
     2881 ttctatcgca gctaaaatgg aagacctaaa aaatcatatc cccattgttt tgtatatttt
     2941 aaagtcaaca acggggatat gattaaaaaa tttcatgttc tgtaaaaatc atgctttgga
     3001 tagttcagat gtgcaatttg gacagcgaac ggctttaatt ggtatagcgg taaagcagaa
     3061 aggacaatct ttggtggttg gagcggcagg tggaacttct tttttccgct gcatttggtt
     3121 gatgattttt accaacaaaa acactgccaa agcaataatg agaaaagtta ttatgctgtt
     3181 aataaacaaa ccatagttaa gcgtgaccgc tcctgcttcc ttggcagctg caacggtatg
     3241 atagggtgga ggtgtatctc cttcttttaa gactgcgaaa agattggaaa aatcgacttt
     3301 acccaaagct aagccaattg gaggcatgat gacgtcatca accagcgatt taacaattgc
     3361 cccaaaagcg gtaccgatga tgaaaccaac agcaagatca accacatttc cacggcttat
     3421 gaactcttta aactcatcga ttaattttga cacctgagaa tccctcccct aaggaagcaa
     3481 aatatctctt ccaagaaaac agaataataa cctttaaaat tccttcaaaa gaagcagatt
     3541 tgttttaata tataactaat caaatggaat ttggatactt ccctcccaaa tactcaggcc
     3601 atttaggaaa atttctacat tgtaaatcgg ttccaacaca atgggataac ctacttcagt
     3661 ggtccaggtt ccttcattgt cgatataggt aaaccgataa gtggtatatc cca
//
LOCUS       DAOSOL010000130         3713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24877_length_3713_cov_3.079005, whole genome shotgun sequence.
ACCESSION   DAOSOL010000130 DAOSOL010000000
VERSION     DAOSOL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24877_length_3713_cov_3.079005"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1058
                     /locus_tag="PK171_04070"
     CDS             87..1058
                     /locus_tag="PK171_04070"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT05400.1"
                     /translation="MKFKILTGYFIFLFQYFEVESMDSQPIYREKIDLDILIQKSQEL
                     MDNLESCTLCPHACRVNRKKGEIGHCRAGMELEVASYGPHYGEEAPLVGYRGSGTIFF
                     HHCPMHCIYCQNWTISQGHGEKISIETLAGYMEELQALGCHNINLVTPTHYLPQILAA
                     IVMVVRNGLNIPIVYNCGGYESTATLKKLDGIIDIYLPDAKYSNKETAEKLSGISGYP
                     EAMKAALKEMHRQVGDLKVDQQGIAQRGLFIRHLVLPNHLAGTQEIIAFIAEKISPTA
                     AINIMAQYYPTYHAFQCPSLNRRLSRQEYEEALKIAHDVSPHFQLNF"
     gene            complement(1071..2606)
                     /locus_tag="PK171_04075"
     CDS             complement(1071..2606)
                     /locus_tag="PK171_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HOT05401.1"
                     /translation="MSTTSLGQVNSISISFFFIFVAATLLITYWASKKSHTTSEFYAA
                     GHGISGFQNGLALSGDFMSAASFLGIAGMVALSGYDGLIYSVGWLVGWPVVLFLIAEP
                     LRNLGKYTYADVVAYRLSKKPVRSAAAVGSIVTVLFYLLAQMVGAGTLIKLVFGLPYE
                     VAIILVGSIMLAYVLFGGMLATTWVQIIKAALLLGGATVLAIWTLYHYGFHFGNLINE
                     AQIQYGIDFLLPGGLVKNPLDTISLGLALMFGTAGLPHILMRFYTVPNSKEARKSVFY
                     ATGFIGFFYILTFILGFGAAVIVGKAAIQTIDQGGNMAALLLAEVLGGQVFLGYLAAV
                     AFATILAVVAGLTLAGASAVSHDLYAGVFKQGIINEKKELRVAKISTLSLGIVAIILG
                     ILFKGQNIAFMVGLAFAIAASANFPALLLSIYWKKFTTQGAVASMITGLGLAIILIFL
                     SPTIWVDVLHHSRALFPLKNPGLFSMAGSFLVGILVSRFTHDLQAEEKFAEETVRSCI
                     GGV"
     gene            complement(2603..2917)
                     /locus_tag="PK171_04080"
     CDS             complement(2603..2917)
                     /locus_tag="PK171_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOT05402.1"
                     /translation="MKKKTNKNKKLDNFAFQELYNRRKAITFTLTLFIVGIYVAYILI
                     NAFDRELLVRKISGGITLGIYIGIGIIIIGWITTGVYVWWANNKYDSLLDRIKRTTGG
                     HS"
     gene            complement(3191..>3713)
                     /locus_tag="PK171_04085"
     CDS             complement(3191..>3713)
                     /locus_tag="PK171_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05403.1"
                     /translation="AYLDIPLVKMAQEQGVLNQEMCRFLTDLNISIDQKAALVNALGW
                     KFEGEKNAEIFTQYLMKKYRFQTKNLPIYALNGSELMCLGYLKLLDDYFHPLEAMKIL
                     EGALKIKPNSFTVNIVTAIARGQVAFDTDWCQIWRFAETVLNNKSLNEDFRPEATSII
                     MDYLILYRDSCFE"
BASE COUNT         1225 a          796 c          682 g         1010 t
ORIGIN      
        1 agatgagatc ctcacgccct tacaaagcga gggctcagga tgaccgatta aagaatttgt
       61 ttgatggtat tttcaggata ggaatagtga aatttaaaat tcttaccggt tattttatct
      121 ttttgtttca gtattttgag gtggaaagta tggattctca accaatttat cgagaaaaaa
      181 ttgaccttga tattcttatc caaaagtctc aggaactgat ggataatctc gagtcctgca
      241 ctctctgtcc tcatgcctgt cgggtgaatc ggaaaaaggg tgaaattgga cattgcagag
      301 cggggatgga attggaagtt gcctcctatg gacctcatta tggggaagaa gctccgttgg
      361 tagggtatcg aggttcaggc actatttttt tccatcattg tccgatgcat tgcatctatt
      421 gtcaaaattg gactataagc caaggccacg gcgaaaaaat ctctattgaa actttggctg
      481 gttacatgga ggaactccag gcacttggct gtcataatat taatttggtt actcccaccc
      541 attaccttcc tcaaatcctt gctgctatag tgatggtagt tcgtaacgga cttaatatcc
      601 ccatcgttta taactgcggc ggttatgaaa gtacagctac tctaaaaaag ttggatggca
      661 ttattgatat ttaccttccc gatgcaaaat actctaataa agaaactgcc gagaaattat
      721 ccggaatttc aggatatccg gaagccatga aagctgcgtt aaaagaaatg catcgtcaag
      781 taggagattt aaaagttgat caacagggaa tcgctcagcg aggcctgttt attcgtcatc
      841 tggtccttcc aaatcatctc gctggaaccc aggaaatcat cgcttttatt gccgaaaaga
      901 tatctccaac tgctgccatt aatatcatgg cgcaatatta tccaacttat catgcctttc
      961 aatgtccatc attaaaccgt cgactttccc ggcaagaata tgaagaagcg ttgaaaatag
     1021 ctcacgacgt cagtcctcat ttccaactca atttttgatg gttaataatt ttatactcct
     1081 ccaatacagc tgcggacggt ttcctcggcg aatttttctt ccgcttgcag atcatgggtg
     1141 aaccgtgaca ctaaaatacc aaccaaaaaa gatccagcca tggaaaaaag tccaggattt
     1201 ttcaagggga aaagggctcg ggaatgatgg aggacatcca cccagatagt ggggctcaaa
     1261 aaaatgagga taatggccaa ccctaagcca gtaatcatac tagctaccgc tccctgagtg
     1321 gtaaatttct tccaatatat cgacagaagc aaggccggaa aattcgcact cgctgcaatg
     1381 gcaaatgcca atcccaccat aaaagcaata ttttgacctt taaagaggat tcctaaaata
     1441 atggcaacga tacccaatga aagggtggaa atttttgcca ccctcaactc ctttttttca
     1501 ttgataatgc cctgtttaaa aactcccgca tacagatcgt gggatactgc cgaagcacca
     1561 gccaaggtca aaccagcaac taccgctaaa atggtagcaa aagcgactgc tgccagataa
     1621 ccaagaaaaa cttgacctcc caatacttca gctaacagaa gagctgccat atttccacct
     1681 tgatcaatag tttgaatagc tgcctttcca acaatgaccg ctgcgccgaa tcctaaaata
     1741 aaagtaagga tataaaaaaa tccaatgaaa ccagtcgcat aaaaaactga ttttctggct
     1801 tctttcgaat tgggaacggt gtaaaatctc ataagaatat gtggaaggcc ggcagttcca
     1861 aacatcagtg ccaatcccaa agaaatagta tctaaaggat ttttaaccaa tcctcccgga
     1921 agcagaaaat caattccata ttgaatctgg gcttcattaa taagattccc aaaatgaaat
     1981 ccataatgat agagggtcca aatcgccaaa actgtggcgc cgcccagcaa aagtgctgct
     2041 ttgataattt gaacccaggt agtggctagc atcccgccaa acaaaacgta ggcaagcata
     2101 atcgaaccaa ctaagataat cgctacctca taaggaagac caaaaaccag ttttattaag
     2161 gttccggcac ccaccatttg agcaagaaga tagaaaagaa ctgtcacaat acttccaaca
     2221 gctgctgcgc ttcgaaccgg ctttttgctc aaccgatagg ccacgacatc cgcataggta
     2281 tatttcccta aatttctgag tggctcagcg atgagaaaaa gcaccaccgg ccagccaacc
     2341 agccaaccca ctgaatatat cagaccatca taaccactca gagccaccat gccggcaatt
     2401 cccaaaaaag atgccgcgct cataaaatct cccgaaagag ccaaaccatt ttgaaaacca
     2461 ctgataccat gtccagcagc atagaactca ctggtagtat gagatttctt tgaagcccaa
     2521 taggtaatga gcagagtagc tgccacaaat ataaaaaaga aggaaattga aatcgaattg
     2581 acctgcccca agctggttgt actcatgagt ggcctcccgt agtcctttta attctatcaa
     2641 gcagtgaatc gtatttgttg ttcgcccacc aaacgtaaac tccagtggtg atccatccga
     2701 taatgataat tccaatgcca atataaatac ccaaggtaat accaccagat attttccgaa
     2761 ccaataattc ccgatcaaaa gcattaatca aaatatatgc aacataaata ccaacgataa
     2821 acaaagttaa agtaaaagta atggcttttc ttcggttata caattcttga aaggcaaaat
     2881 tatctaattt tttatttttg ttggtctttt tcttcataag cttcccctca cattaaaatc
     2941 aaattgtcgg ctttgaagtg aatcaataat tcataatatt gaaacgataa aattgtctga
     3001 gacaattaaa atcttatctc ggtaaaaata tggggcttat aaaacagtgg gcaaatggtt
     3061 aaatgaactt agaggaaaaa ttaacttggt ggaggatgaa actggcgaat catagtaaaa
     3121 atcaactgta ttcccctgcc tttttatttt caatttccat ttattatacc tcttattgtg
     3181 aaaaaagagg ttattcaaaa caactatccc ggtataaaat taaataatcc atgataatcg
     3241 atgtggcttc cggacgaaag tcttcattaa gcgatttatt gttcaatacc gtttcagcaa
     3301 aacgccaaat ttggcaccaa tcagtatcaa aagcaacctg gcctcgagct atagcagtta
     3361 caatatttac agtaaaactg tttggcttga ttttcaaagc tccctcaaga attttcatag
     3421 cttctaaggg gtgaaagtaa tcatcaagaa gtttgagata cccaagacac atcaattcac
     3481 taccatttaa ggcataaatt ggaagattct tggtttgaaa tcgatatttt ttcataagat
     3541 attgagtaaa tatttcagca ttcttttccc cctcgaattt ccaccccaag gcattaacta
     3601 aagcggcttt ttgatcaata gaaatattga gatcagttaa gaatcgacac atttcttggt
     3661 taagaacccc ttgttcttgt gccattttaa ctagaggaat atccagataa gct
//
LOCUS       DAOSOL010000131         3701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_24988_length_3701_cov_3.862041, whole genome shotgun sequence.
ACCESSION   DAOSOL010000131 DAOSOL010000000
VERSION     DAOSOL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24988_length_3701_cov_3.862041"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="clpX"
                     /locus_tag="PK171_04090"
     CDS             <1..957
                     /gene="clpX"
                     /locus_tag="PK171_04090"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT05404.1"
                     /translation="LEENEVEIEKSNILLIGPTGSGKTLLARTLAKMLNVPFAIADAT
                     TLTEAGYVGEDVENILLRLLQNAEFNLKKAEKGIIYIDEIDKIARKSENPSITRDVSG
                     EGVQQALLKILEGTIANIPPQGGRKHPHQEFIPMNTANILFICGGAFVGLERIVEARM
                     KEKKIGFGAFEANQTKLQENPLIDVQPHDLMKFGMIPELVGRIPIICALDELNVEDLV
                     RILTVPKNSLLKQYQHLLRLDGVDLRFTDGALKQIAIEAMEKKTGARGLRAIMEKLMT
                     KVMFECPDRKDIKKLTVDKDMVIKNQTNDIYVDNQLIQDKPA"
     gene            972..3629
                     /locus_tag="PK171_04095"
     CDS             972..3629
                     /locus_tag="PK171_04095"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT05405.1"
                     /translation="MNNRTPIPQVYDASHTETKWYEYWEKNGYFSPDPQRSNPFCMVI
                     PPPNVTGFLHMGHALNLTLHDILTRYRRMKGDQTLWLPGTDHAGIATQNVVEKQLAKE
                     GLDRHQLGREEFIQRVWQWKDQYHDRIVKQLKSMGASCDWTRERFTFDEGLSAAVKEV
                     FVRLFDEGLIYRGEYIVNWCPRCETAISDIEVDYRDVPSFLYYVRYPIKEHPEQYLVV
                     ATTRPETILGDVALAVHPEDERYRQVVNSTVILPIIGRDMSIIQDEYVDPQFGTGVLK
                     VTPAHDMNDFDLGKKHHLPVVAVIDPHGVMNENAGIFAGMAREQCREAIVEKLKSLDL
                     IEKIEEYSHWVGHCYRCNTQVEPLVSKQWFVKMKDLAQPAIQAVEEGRVEFIPERWNK
                     IYFEWMNNIRDWCISRQIWWGHRVPVFYCQECGHYFADRGLPDHCPKCQGKVVQDEDV
                     LDTWFSSALWPFSTLGWPETTPDLKTFYPTSVLITAFDIIFFWVARMIMMGLKFMQDV
                     PFRKVYITPLVRDAYGKKMSKSSGNAIDPLEVARQLGSDSLRFALAWLTVQGRDIHLS
                     MERIEASRNFMNKIWNASRFVLMNLEEDFVPVELNNEAHLSLKDRWIISRFQQTIEQA
                     SLALDDYRFGEYVQIIYDFIWGEFCDWYIEWSKKDLYQGSPEEKRKTQSVLVQVLSGI
                     LQLLHPVAPFITEEIWHSLPMRNDNDALIISDWPVSEKPWFNDQSEKAIGSIQNLIRE
                     IRFLRAELEISPAETCRVQLGFHQSDNRVIIEEHLGYIEQLAKCEIIKTDVEISKPGG
                     AVTGQVDGIDIFLMIEGLVDIKRELQRLNKKHKVLSEDVERIQKRFEKPDFLEKAPQE
                     VIEKDQIRLEKLLSEAQRLQKLIEGIMNQ"
BASE COUNT         1066 a          692 c          900 g         1043 t
ORIGIN      
        1 ctagaggaaa atgaagttga aattgaaaaa agtaacattc tattgattgg gccaactggt
       61 tcaggaaaaa ctcttttagc ccgaaccttg gccaagatgt tgaacgttcc ttttgcaatt
      121 gctgatgcaa ccacccttac tgaggcgggt tatgtgggcg aagatgttga aaatatcttg
      181 cttcgccttt tgcaaaatgc tgaatttaac ttaaaaaagg ccgaaaaggg aataatctac
      241 atcgatgaga ttgataaaat tgctcggaaa tctgagaatc cctcaattac ccgtgatgtg
      301 tcgggagagg gagtgcagca ggccttgttg aagattttgg agggtaccat tgccaatatt
      361 cctcctcaag gggggagaaa acatcctcat caagaattta tcccgatgaa taccgccaat
      421 attctgttta tttgtggtgg agctttcgtt ggattggagc ggattgttga agctcggatg
      481 aaggaaaaga aaatcggttt tggagctttt gaagccaacc agaccaagct tcaggaaaat
      541 cctttaattg acgtacagcc gcatgacctg atgaaatttg gtatgattcc cgagctggtg
      601 gggagaattc ccatcatctg tgctttggat gagctcaatg tagaggattt ggtacggatt
      661 ttaaccgtac caaaaaattc cttgctaaaa cagtatcagc atcttctccg cctggatgga
      721 gttgacctcc gtttcaccga tggcgcctta aaacaaatag ccattgaggc catggaaaaa
      781 aagaccgggg ctcgtggttt gcgggctatc atggaaaagt tgatgaccaa agtgatgttt
      841 gaatgccccg atcggaaaga tattaaaaaa ttaactgttg ataaagacat ggtcataaaa
      901 aatcaaacca atgatattta tgtggataat caactcattc aagataagcc agcctaaaga
      961 aaggaataaa aatgaacaat cgaacaccga ttccgcaggt ttatgacgct tctcacactg
     1021 agaccaaatg gtatgagtat tgggaaaaaa acggttattt ttctccagat ccgcagcgtt
     1081 ccaacccttt ttgtatggtt attcctcctc ccaatgtgac cggtttcctt catatgggtc
     1141 atgctctcaa tctgactctt catgatattc tcactcgtta ccggaggatg aaaggtgatc
     1201 aaacgctttg gttgccggga accgatcatg ccggcattgc tacccaaaat gttgttgaaa
     1261 aacagttggc gaaggaagga ttagatcgcc atcaactggg tcgagaagag tttattcagc
     1321 gggtttggca atggaaagac caataccacg atcggattgt aaaacagctg aaaagcatgg
     1381 gtgcttcctg cgattggacc cgagagcgtt ttacctttga tgaaggattg tcagctgcgg
     1441 taaaagaggt gttcgttcga ctttttgatg aagggctgat ttaccgggga gagtatatcg
     1501 ttaactggtg cccccgttgt gaaacggcca tttccgatat cgaagttgat tatcgtgatg
     1561 ttccatcatt tttgtattat gttcggtacc ccatcaagga gcatcctgaa cagtacttgg
     1621 tggttgctac cacccggccg gaaaccattt tgggagatgt ggctttagcc gttcatcccg
     1681 aagatgaacg ataccgccag gtagtcaatt ccactgttat ccttcctatc atcggaagag
     1741 atatgagtat catccaggat gaatatgttg atccgcagtt tggaaccggg gttctcaagg
     1801 taacaccggc ccacgacatg aacgattttg atttggggaa aaaacatcac cttccggtag
     1861 ttgcggttat cgatcctcac ggagtgatga atgaaaatgc tgggattttt gccggtatgg
     1921 ctcgagaaca atgtcgagag gcaattgtcg aaaagctgaa gagtttggac ctcatcgaaa
     1981 aaattgaaga atattctcat tgggttggtc actgttaccg atgtaatact caggtagagc
     2041 ctttagtatc caaacaatgg tttgtgaaaa tgaaagattt agctcaaccg gcaatccagg
     2101 cagttgaaga gggtcgggtt gagtttatcc cggaacgatg gaataaaatt tattttgaat
     2161 ggatgaataa tattcgggat tggtgtattt cgcggcagat ttggtggggc catcgggtgc
     2221 cggtttttta ttgtcaagaa tgtggtcatt attttgccga ccgagggtta cccgatcatt
     2281 gtccgaaatg ccagggaaag gtagtccagg atgaagatgt tctcgatacc tggtttagct
     2341 cagctctttg gcctttttct actttggggt ggccagaaac aactcctgac ctaaaaacct
     2401 tttatcctac ctcggtttta atcacggcat tcgatattat ctttttctgg gttgcgagga
     2461 tgattatgat gggattgaag tttatgcagg atgttccctt tcgaaaggtt tacatcactc
     2521 ccttggtccg tgatgcctat gggaagaaga tgagcaaatc gtcaggtaat gccatcgatc
     2581 ccttagaagt tgctcgtcag ctgggatcgg attctttacg ctttgctctt gcttggttga
     2641 cggttcaggg tcgagatatt catctctcca tggaaagaat cgaagcctcg agaaatttca
     2701 tgaataaaat ctggaatgct tcccgctttg tcttgatgaa tcttgaagag gactttgttc
     2761 cggtcgaatt gaataatgaa gctcatctca gtttgaaaga ccgctggata atctccagat
     2821 ttcaacaaac catcgaacag gccagcctgg ctctcgatga ttatcgattt ggtgaatatg
     2881 tccagattat ttatgatttt atttggggtg aattctgtga ttggtatatc gaatggagta
     2941 aaaaagacct ctaccaggga agtccggaag agaaaaggaa aacccagtcg gttttagttc
     3001 aagtgctttc gggaattctt caacttcttc atcccgtagc accatttatt actgaagaaa
     3061 tctggcactc tctcccgatg agaaatgata atgatgcgct tatcatatcc gactggccag
     3121 tttcggaaaa accgtggttc aatgaccaga gtgaaaaagc tatcggatca atccagaatt
     3181 tgatacgaga aatacgattt ttaagagcag aactggagat ttctcccgct gaaacctgtc
     3241 gggtgcagct tggttttcat caatcggata atcgggttat aatcgaggaa catttgggat
     3301 acattgaaca actggcaaaa tgtgagataa taaaaactga tgttgaaatc tcaaaacccg
     3361 gaggcgcggt gactgggcaa gttgatggga tagatatctt tttaatgatc gaaggattgg
     3421 tggatattaa gcgggaactg cagcggttaa ataaaaagca taaagttctt agcgaggatg
     3481 tggaaagaat tcaaaaacgt tttgaaaaac cagatttctt ggaaaaagct ccccaagaag
     3541 tgatagaaaa agatcagatt cgtttagaga aattattaag cgaggcgcaa cgcctccaga
     3601 aattaataga ggggataatg aaccaatgaa gcaatttcgg aaaaggcggg acccagttga
     3661 gttgattatt cttcttctcg gtggtttttt agtggtaatg c
//
LOCUS       DAOSOL010000132         3694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25051_length_3694_cov_4.404507, whole genome shotgun sequence.
ACCESSION   DAOSOL010000132 DAOSOL010000000
VERSION     DAOSOL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25051_length_3694_cov_4.404507"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..977
                     /locus_tag="PK171_04100"
     CDS             264..977
                     /locus_tag="PK171_04100"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOT05406.1"
                     /translation="MNKKYLFTFLIVWIIFVLVFSADAEQYDQERQLMVEKQIKQRGV
                     VDPQLLDAMLKVPRHLFVPENLVSLAYNDTPLPIGYGQTISQPYIVALMTDSLKVEEG
                     FKVLEIGTGSGYQAAVLAEIGCQVFTVEVIKILADTAQERLKSLGYPEINVRWGDGYF
                     GWKEEAPFDSIIVTCAIDHVPPPLIEQLKEGGKMVIPVGPPYSLQTLWLFTKEKDQLT
                     SENLGGVIFVPLLREVREE"
     gene            complement(1011..3332)
                     /locus_tag="PK171_04105"
     CDS             complement(1011..3332)
                     /locus_tag="PK171_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05407.1"
                     /translation="MKMRGETTKSFQLFIISLCSLAVEMVLFRWFAFLAGFHFVSLII
                     SLALLGYGASGALFRFYPLSMKPLIPLCFIFGVAAVLVGLVFLPMDVYEFWTYSIYKI
                     NFILLIIITSIPFFYHGLYQIHLFDEYPDHFPRFYSMNLLGSAAGVIIGVVLLMFFNE
                     IQSLVTIMGIGCLNLWKQKKLFFIFILIIIILFFLPLTLKISDYSPTRQLRLIPDNQL
                     LSTYRNPSQHLEVFFTPYSRAATGLSMRYRDVPPSSYSLITDYTHQVIFPHQPSEEFI
                     YNTLFALPLRVFKPDEVLGIECDWGYEMYAMYYSSVSGTIVTSSPLYREFLKNLRPSI
                     PYEIEVAFPRKYLSQTQSHYDLIFLQIPVGNGEIFPGAFSMKENYLFTIEGIRSVVNH
                     LNDDGKLVISFFPQTPPMVLLKLMDLVSQVWGDPKLLQNRLIIIKNLDFAQMIISIQI
                     FDQMEIEKIRQDVSVYGFDFIYYPDIDEEEVEKVFQTQKLNYRIVMDYFQDPKETLQK
                     SFYNVKAPRDNKPYFFHFFQWNQFKETLYNLGKRWLPFGGAGFLLVLFIFLVITALSL
                     LFILIPGRAFMGRKKLHFAGGKELELAAIITGIGYMLIEIPVIVQLEMIIGFPIYTFA
                     LVLTILLVFSGLGSWYVEKIRSNRFFLKMVFVHPLIILGFFLYINYLRSWLLELPGIL
                     SLLVVLLPFSLLAFTAGMPFPILSRLTHQRNPDFFQVVFAWNGFMSVVASLLSHFTAI
                     EYGIHFPYFLSIPLYGGFWVIVHFLNKSLHPIP"
BASE COUNT         1304 a          660 c          733 g          997 t
ORIGIN      
        1 caccaaaaag agggatgtat ctatttgact atccatggtt ggattcttag taatgacgga
       61 tttagattgc tattttcatt gtcatctgct gccacataag tagcatgagg gttttcccta
      121 aaatagaggt atggatcaaa aaaattgcag gaaaatcgcc agattaatgg aatgaagaat
      181 tcgtgtttta accttcatta agcgatataa taaagatatt ggtttttcat aatatcaata
      241 atttcttaag gcaggggttt acaatgaata aaaaatattt attcactttt cttatcgtat
      301 ggataatttt tgtattggtt ttttctgctg atgctgaaca atatgaccaa gaacggcagc
      361 ttatggttga gaagcagatc aaacaacgag gggttgttga cccgcagctc ctcgatgcaa
      421 tgttaaaagt acctcgtcat ctctttgttc cagaaaattt ggtttcctta gcctataacg
      481 atacaccgct cccaattgga tatggtcaga ccatttccca gccctatatc gtcgcattga
      541 tgaccgatag cttaaaggtc gaagaaggat tcaaagtttt agaaatcgga actggttctg
      601 ggtatcaagc agccgtgtta gctgaaattg gctgtcaagt ttttactgtt gaagtcatca
      661 aaattttagc agacactgct caggaaagat taaagagtct tggttatccg gaaataaatg
      721 tccggtgggg cgatgggtat tttggctgga aagaagaagc tccttttgat tcaatcatcg
      781 ttacctgtgc catagatcac gttccgcctc ccttgattga gcaactcaaa gagggaggaa
      841 aaatggttat tcccgtcggc ccaccataca gcttacaaac tctatggctt ttcaccaaag
      901 aaaaggatca attgacgtcg gaaaatttag gaggagttat tttcgtccca ctccttcgtg
      961 aagttcgaga agagtagaaa acatttacta gaccatcttt ccacatttgt ctaaggaatg
     1021 ggatggagcg atttatttaa aaaatgtaca atcacccaga aaccaccata tagcggaata
     1081 gataagaaat aagggaaatg aattccgtat tcaatagcag taaaatgaga aagtagagat
     1141 gcgacaaccg acataaaacc attccaggca aaaaccacct gaaaaaaatc tgggtttctt
     1201 tggtgagtca gtctcgataa aatcggaaac ggcattccgg ctgtaaaggc taataaagag
     1261 aagggtagca aaacaaccaa taaactcaag ataccgggca attccaataa ccagctacga
     1321 agataattta tatataggaa aaatcctaaa attatcaagg ggtggacaaa aaccatcttg
     1381 agaaaaaatc gatttgaccg aattttctct acataccaac ttcctaatcc agaaaaaacc
     1441 aataaaatag tgagaactaa agcaaacgta taaataggaa atccaatgat catttctaac
     1501 tgaacaataa ccggtatctc gatgagcata tagcctatgc cggttataat tgccgctaat
     1561 tcaagttcct tgcctccggc aaaatgcagt ttttttcttc ccataaaagc ccgtccggga
     1621 atgagaataa agaggagaga aagagcagtt ataaccagga aaataaaaag aaccaataaa
     1681 aaacctgctc ctccaaaggg aagccagcgt ttccccaagt tatagagggt ttccttgaat
     1741 tggttccatt ggaaaaaatg aaaaaagtaa ggtttattgt cacgaggggc tttgacatta
     1801 taaaaagatt tctgaagggt ttcttttgga tcctgaaaat agtccataac tatccgatag
     1861 tttaatttct gagtttgaaa tactttttca acttcctctt catcgatatc cggataataa
     1921 ataaagtcaa aaccatagac cgaaacatct tggcgaattt tttcaatttc catctggtca
     1981 aaaatttgaa tggatataat catttgagca aaatctaagt ttttaattat aatcagccga
     2041 ttctgaagta attttggatc cccccaaact tgagaaacca aatccatcag cttcaacaga
     2101 accatggggg gagtttgagg aaaaaaagat atgactaact tcccatcatc gtttaaatga
     2161 ttcacgaccg aacgaattcc ttcgatagta aaaagataat tttctttcat tgagaacgct
     2221 ccggggaaaa tttctccgtt accgacggga atctggagaa aaatcagatc gtagtgagat
     2281 tgagtttggc tgagatattt tcttggaaaa gcaacttcaa tttcataagg aattgaaggg
     2341 cgaaggtttt ttaaaaattc ccgataaagt ggtgatgagg taacgatagt tccggaaaca
     2401 ctggaataat acatagcata catttcatat ccccaatcac attcaatacc gagcacctca
     2461 tcaggtttaa aaactcgcaa gggaagagca aagagggtgt tataaatgaa ttcctcgctt
     2521 ggttgatgag gaaaaattac ctggtgggta taatcagtta tgagcgagta ggatgaggga
     2581 ggaacatctc gataacgcat gcttaacccc gttgcggctc ttgaataggg ggtgaaaaaa
     2641 acctctaaat gttgagaggg atttcgatag gttgataaaa gttggttatc cgggatcagt
     2701 cttaattgac gagtggggct atagtcagaa atttttaatg tcaatggtaa aaaaaacaga
     2761 atgatgatta ttaaaataaa tataaaaaag agtttttttt gtttccaaag atttagacat
     2821 ccaataccca tgatagtaac taaactttgg atctcattaa aaaacataag caaaacaaca
     2881 ccgattataa ctccagccgc cgatccaaga agattcatgg aataaaaccg agggaaatgg
     2941 tcgggatatt catcaaaaag gtggatctga taaaggccgt ggtaaaaaaa cgggattgag
     3001 gtaataataa taagtaaaat aaaattgatt ttatagatgg aataggtcca gaattcataa
     3061 acatccatag gcaaaaaaac taatccaacc aacacagctg ccactccaaa gataaaacag
     3121 agaggaatca aaggtttcat tgaaagcgga taaaagcgga aaagagctcc agaagcacca
     3181 taacccaata aagccagcga tataataagc gagacaaagt gaaaaccagc caaaaaagca
     3241 aaccaacgaa agagaaccat ttcaactgcc aaagaacata gtgaaattat aaaaagctgg
     3301 aaagatttgg tggtttcccc tctcattttc attcaatccc agactgtttt tctaattgta
     3361 tcatagatag aaatgggttt aatattgatg gggtattaaa gaaattaatc ctttctttaa
     3421 ggaaaagtat tttttaaaaa ccttcatgct gctttcgcag caccagatga ggatgaaaat
     3481 acctatctta atccgtcatt gcgaggagac caaccgattt ttggttgaac gacgtgacaa
     3541 tccctgccac ccagcttgtc attctgagga gtccggtgtc ttctgccgga cgacgtgaga
     3601 atctcatcct tttaaatctt tccaaaatta aaacatgaaa gatgagatcc tcacgccctt
     3661 acaaagcgag ggctcaggat gaccgattaa agaa
//
LOCUS       DAOSOL010000133         3689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25101_length_3689_cov_3.452669, whole genome shotgun sequence.
ACCESSION   DAOSOL010000133 DAOSOL010000000
VERSION     DAOSOL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25101_length_3689_cov_3.452669"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1470)
                     /locus_tag="PK171_04110"
     CDS             complement(217..1470)
                     /locus_tag="PK171_04110"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOT05408.1"
                     /translation="MDHYNKNRMEAIKVVEALRTGVPTRISTRTLPDLRKNLTETLRA
                     DLDLLTTGKIPRGRLIWGQYGQGKTHVLTTTEHLALDRQFAVSFVSLSREVSCHNLFH
                     FYGRAASRLRTPDSSMFGLERALSKKHASDLQKTSILVPDRYIHPLPAIVIENYLHSA
                     GEEQNLLYGDLMGTRIPLTELKRIHRQNYSEKFPTFETSFRMIDHAKAYFGCLADTIV
                     FCGYRGWVILIDELELVGRLGSQSRLKAYQNLQWLLNWSNAHHYPIYVIAAAATSLQS
                     EMWYGGKDDRTLMPKLAEEKFGKSAYNEITEFFEYALSPQSLVVTPASEEAISELLKR
                     IVELHGKAYSWEAKLDVNSLVRNLGETTLRTYIRATLESLDHQFLYHETIQTTPFNFK
                     EKPLTEDNSFFSNPYSQENNTENNN"
     gene            complement(1472..2671)
                     /locus_tag="PK171_04115"
     CDS             complement(1472..2671)
                     /locus_tag="PK171_04115"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HOT05409.1"
                     /translation="MLIEEKDIELLKRGEPPINSDILKTINIGRELWLDFFKEYYLNN
                     FIYQGGSKVKVVVGSEGTGKTHLLRCIEQEARDQGYATVFFSLRDFYFKLNDLTSLYK
                     MIVSQIDLEELVRGLCRKTASMLGYDSNHYDGSERLLPIMVEDGMTKVTAEKEIRNTA
                     SQVFKDADFGSSFSAFAYTVVKERMIKGKDGTLTTALRWLSGEKLERQERQTTYLFEK
                     LQKSNARYWLNSLIRLLKYAGWSGLLVLIDDVDIITRRSPETARYYYTPNAIKDVCEI
                     IRQLIDDTELLESFVMILSGRYPMLEDEKRGFKSYEALWMRLQSGLVTVNRFNRFADI
                     VNMDDFVKALNDQLETKLTSKMNELFTSAGYERKYLEELPDTSTMSKLRAIVMENAMF
                     MKKKEGY"
     gene            complement(2661..3479)
                     /locus_tag="PK171_04120"
     CDS             complement(2661..3479)
                     /locus_tag="PK171_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05410.1"
                     /translation="MELNPKESSPPISWNLLEDNTHILAKSVKHLKKAQKKWDFRLFE
                     QMKQQFQESLNNIKESWNALEPYVENEMTLHKEYLATEQFIKDFEHELAESNILFQGE
                     FPDYIFPPFHLHFDLENYHVLLILGRKSQRFSILQPRELAILIANEYKTIYNRRFNSK
                     NFLKDLLNAYKIANCLSFKQKEALWGKAVSLDKIYEILTVRRSTHQEYPKILFQFELG
                     LLKERFDLSLNEEYVFEFGFTRSARKALVVVDSQGRESRISTLTIYKEERPHVD"
BASE COUNT         1091 a          700 c          674 g         1224 t
ORIGIN      
        1 ggatctcatt ctgggctttc accctcatcc tcaccttctc ccatcaaggg agaagaaatt
       61 ctagtgatat gtaatcctga gcccttactt ttcgatggta tgatggtctc atcttttaca
      121 aattgtggaa ttggggaaac gtaattcatt ccaaaaccat tctctatctc attttattga
      181 ggggaaaatg tttaataatg gtgattatgc aaatcttcag ttattatttt ctgtgttgtt
      241 ctcttgacta tatggattag aaaaaaacga attgtcttca gttagaggtt tttctttaaa
      301 attgaacgga gtggtttgaa tggtttcatg gtagaggaac tgatgatcta agctctcaag
      361 agtggctcga atataagtac gaagcgtggt ctcacctaaa ttacgaacta aactgttaac
      421 atcaagcttt gcctcccagc tataggcttt tccatgtaat tcgacgattc gttttaagag
      481 ttctgaaatg gcttcctctg aagctggagt gacaactaaa ctttgagggc tcagggcata
      541 ttcaaaaaat tctgttattt cgttataagc gctttttcca aacttttctt cagctaattt
      601 tggcataagg gtacgatcat ccttccctcc ataccacatt tcactctgaa ggctggttgc
      661 tgctgcagcg ataacgtaaa ttggatagtg atgagcattc gaccaattaa gcaaccattg
      721 aagattttga taagctttga gtcgggattg actacccaag cgtcccacaa gttctaattc
      781 atcgataaga atgacccacc cgcgataacc acaaaagact atcgtgtctg ctaaacatcc
      841 aaagtaagct tttgcgtggt caatcattcg aaagctggtt tcaaaagtgg gaaatttttc
      901 agagtagttc tgacgatgga ttcgcttcaa ttctgtcaat ggaatacgag tacccattaa
      961 gtctccataa agcaagttct gctcttctcc agctgaatgt aaataatttt ctataacaat
     1021 tgcagggaga ggatgtatat atctgtctgg tacaagtatt gatgtttttt gaagatcgct
     1081 ggcatgtttt tttgataaag cacgttccag tccgaacatc gaagaatcag gagtccttaa
     1141 acgagaagca gcccggccat aaaaatgaaa aagattatgg caggatacct cacgactgag
     1201 tgagacgaag ctcactgcaa attgtcgatc aagagccaaa tgttcggtag tggttaaaac
     1261 gtgggttttg ccctgaccgt attgacccca aattaatcta ccacgaggta tttttccggt
     1321 ggttaataaa tcgaggtcag cacggagggt ttcggtgagg tttttacgaa gatcaggtaa
     1381 ggttctcgtc gaaattcgcg ttggtactcc agttcggagg gcttcaacta ctttaatggc
     1441 ttccatacga tttttattat aatggtccat atcaatatcc ctcctttttt ttcataaaca
     1501 tggcattttc cattacaata gcgcgaagct tactcatggt cgaggtgtca gggagttctt
     1561 caagatattt ccgttcatat cctgccgatg tgaaaagttc attcattttg gaagttaatt
     1621 ttgtttctaa ttggtcattc aatgctttta cgaaatcatc catatttaca atatcagcaa
     1681 aacgattaaa tcggttaact gttactaaac ctgattgtaa gcgcatccaa agagcttcat
     1741 aacttttaaa tccccttttt tcatcttcga gcatagggta tcgaccagat aatatcatta
     1801 caaaactttc taataactct gtgtcatcaa taagttgtcg tattatctcg cagacatctt
     1861 taatggcatt tggtgtgtaa taatagcgag ctgtttctgg agaacggcgg gttatgatat
     1921 ctacatcatc gatgagaaca agcaaaccac tccatccggc atacttcaat agacgaatca
     1981 aagaattaag ccaataacga gcattcgatt tttgcagttt ctcaaataaa taagtcgttt
     2041 gtcgttcctg tcgctctaat ttttcaccag acaaccatcg taaagccgtt gttaaggttc
     2101 catccttacc ttttatcata cgttctttaa ctactgtata ggcaaaagcc gagaatgaac
     2161 taccgaaatc agcatcttta aaaacttgac tggcagtatt gcgaatttct ttttctgctg
     2221 taactttcgt cataccatct tcaaccatta taggaaggag gcgttccgat ccatcataat
     2281 gattgctatc atatccaagc atactggctg ttttccggca aaggcctcgg actagctctt
     2341 ctaaatcaat ttgtgagact atcattttgt acaatgaggt aaggtcattt aatttaaaat
     2401 aaaaatcacg gagtgagaaa aaaacggtgg cgtacccttg atcacgtgcc tcttgttcta
     2461 tgcagcgcaa taagtgagtt ttacccgttc cttctgatcc aaccacaacc ttgaccttgc
     2521 ttccaccctg ataaataaaa ttattgagat agtattcttt aaaaaaatcc agccatagtt
     2581 cccgaccaat gttaatagtt tttagaatat cagaattaat tggaggttct cctcttttta
     2641 aaagttcgat gtctttttct tcaatcaaca tgagggcgct cctccttgta aatggttaat
     2701 gtactgattc gactttctct tccttgacta tccacaacga ctaaagcttt ccttgcgctt
     2761 cgagtaaatc caaactcaaa tacatattct tcattcaaag aaagatcgaa tctttccttt
     2821 aaaagaccta attcaaattg aaagagaatt ttcggatatt cttggtgagt tgaacgtctt
     2881 actgtgagta tttcatatat tttatcaaga gaaaccgctt ttccccaaag agcttccttt
     2941 tgtttgaatg aaaggcaatt ggctatctta taggcgttga gtaaatcctt aagaaaattc
     3001 ttagaattaa aacgccggtt atatattgtc ttatattcat tggcgattaa aattgccaat
     3061 tctcgtggtt gtaaaataga aaaacgttga ctttttctac ccaggataag aagaacatga
     3121 taattttcca aatcaaaatg aaggtgaaag ggtggaaaaa tataatctgg aaactcacct
     3181 tgaaaaagaa tatttgattc agctagttcg tgctcaaagt cttttatgaa ttgttcagtg
     3241 gctaaatatt ctttatgaag agtcatttcg ttttcaacat aaggttccag ggcattccag
     3301 gactctttta tattatttaa agattcttga aattgttgtt tcatctgttc aaacaatcga
     3361 aagtcccatt ttttttgagc ttttttcagg tgtttaacac ttttagctaa aatatgtgtg
     3421 ttgtcctcga gtaaattcca gctgataggt ggtgaacttt cttttggatt caactccata
     3481 tgagtatcct cccttattag gttttaatac tttttagact aatttaaatt gctttgtttc
     3541 atatataccc tgaataatta aaatatgcaa tatgaatatc tatctatact atcgaggata
     3601 aactttgggt aatatccagg ttccatctaa ggcatcacgt tttggccagt atattcgcag
     3661 ggtaatattg aataatccac tgggaggaa
//
LOCUS       DAOSOL010000134         3686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25127_length_3686_cov_3.923988, whole genome shotgun sequence.
ACCESSION   DAOSOL010000134 DAOSOL010000000
VERSION     DAOSOL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25127_length_3686_cov_3.923988"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..597)
                     /locus_tag="PK171_04125"
                     /pseudo
     CDS             complement(184..597)
                     /locus_tag="PK171_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(645..803)
                     /locus_tag="PK171_04130"
     CDS             complement(645..803)
                     /locus_tag="PK171_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05411.1"
                     /translation="MIFMEIPIDISEKISQLTTASSRVISCHLGSGSNICAIQDGKSL
                     DISSGFTP"
     gene            complement(1325..2263)
                     /locus_tag="PK171_04135"
     CDS             complement(1325..2263)
                     /locus_tag="PK171_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT05412.1"
                     /translation="MKCTACKDEAILRIKRHHAAFCQRCFKMFIQKQTIQAIKKYQMF
                     SKKEPVILAVSGGKDSMALWSVLTDIGNQITAIHLDLGIPNFSKQSLQAVQQFAQDKG
                     LPLIVIHLKELLGHSLPELQNKIPRPACSVCGLIKRYLLNHYAFHHDFSVLATGHNLD
                     DEAATLLGNLLRWREGYLQRQSPCLPSDYPGLVKKVKPFYTLTDQEILWYAQQMKIPF
                     CDSICPLSQKASSLDFKKALNVIEEQSPGTKHYFLFHYLEKKQFLFPEKQPKVVLNNC
                     SSCGMPTTQEKCSFCSILETAQSKSQLFIGKNDIEL"
     gene            2782..2858
                     /locus_tag="PK171_04140"
     tRNA            2782..2858
                     /locus_tag="PK171_04140"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2816..2818,aa:Met,seq:cat)
     gene            2898..>3686
                     /gene="tilS"
                     /locus_tag="PK171_04145"
     CDS             2898..>3686
                     /gene="tilS"
                     /locus_tag="PK171_04145"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT05413.1"
                     /translation="MKKYPYLLKTVSRLIQEQELIVTGDRVLIAFSGGPDSTALSDIL
                     GKLCEEFKFQLAFFHLNHGLRGQEALRDQNFCVEWAKKRNFEIFIEQYDVKAYKKELS
                     ISLEEAARKVRYQKLKEVAELWKADKIALGHHRNDQVETILMNIIRGTGINGLRGMPI
                     RNGKFIRPLLRTSLDEIHRYLEEQHLAFVIDSTNLDQSFLRNRIRNGLIPLLKQDYNA
                     KIEEIIFRMGLNIQESLSIIPEEKWSIEKKGRLFRLPVSSLVETS"
BASE COUNT         1124 a          668 c          730 g         1164 t
ORIGIN      
        1 gtgaggatct catcttttag tttctttctt cataaatact ttaaaggatg agattgccac
       61 gtcactccgt tcctcgcaat gacggatcag taaaaaatat tataaatatt aaaattataa
      121 aaataaatct ttgtattcga gattcttagg tctgaagata agcataagtt tctcgagcta
      181 tcattaattc ctcgttgatt ggaataaccc agatctcgac gtttgatttt tcatcatgga
      241 tcggtttagg gattggggat ggtgaagaat tttttcatgg tcaattgaaa taccgagctg
      301 atccaaataa ctacaaattt tctcacgaat gaaagaatcg ttttcaccaa ttcctccagt
      361 aaatattagc gaatcgagcc cgttcattaa agtgataaat gaaccaatat acttttttac
      421 ctggtataca aaaacatcaa ttgcaagcaa agcccgctcg tttccatctc gggcagcttt
      481 tttcaatacc caaatttagg tgctttgtcc ggagatacca aggaagcccg acttctttac
      541 caataattca ttgatcgcag aatagctcat cttctctttt tcttggaggt aggtaataac
      601 ctaggatcaa aatctcccga tcgagatgac ataataattc cactttacgg agtaaaacca
      661 ctggaaatat ctaatgattt tccatcttga attgcacaga tgttgctgcc actgcccaaa
      721 tggcaggaaa taacccggct agatgcggta gtcaattgag atattttttc cgaaatatcg
      781 atgggaattt ccatgaaaat caaatttaat aattctgtat ttttgacaaa ggcctaaagg
      841 aagctcatag gtataatgtg ctggggggat gctttggtga aaggtagttt caaaaacacc
      901 tactcagggg acctgggaag aatcctttgg aaaatgtcga cggtgagtaa ggttggtgaa
      961 ttgtgaaccg gtgttacgct ttctaattct cgaagagtac tgattacgtg aaaatcaatc
     1021 agagaagtgg aattgactcg attccctcag atgatgcatt taatacctat tgcctttagt
     1081 cctgttagat cattgagagg ttcatattca attatattgt ttattatggc ttttaaacct
     1141 agtttatgag aacactggga tatggatgtt tgaaaggttt tcccgttaat cttttgttcc
     1201 caaaaagagc catcgcgata caaacgcttt actacccctc gactaaggga aatttcttat
     1261 tctttctcaa tttcatagag ttcgtattta attgaagatc caccgctgtt taaagccata
     1321 attttcatag ctcaatatca ttctttccaa taaaaagttg agatttgctt tgggcggttt
     1381 caagtattga acaaaaggaa catttttctt gagtagtagg catgccgcaa gaagaacaat
     1441 tattgagaac aacctttggt tgcttttctg gaaagaggaa ttgctttttt tctaagtaat
     1501 gaaataaaaa ataatgtttg gttccaggtg attgctcctc gatcacgttc agggcctttt
     1561 taaaatcaag actggaagct ttttgtgaga gagggcagat gctatcacaa aaagggattt
     1621 tcatctgttg ggcataccag agaatttcct ggtcggttag ggtgtaaaag ggttttactt
     1681 tttttactaa accgggataa tcactaggta agcaggggct ttgacgctga agatatcctt
     1741 ctctccatcg aaggagattc cccaaaaggg tggcggcttc gtcatcgagg ttatgaccgg
     1801 tagctaaaac cgaaaagtca tgatgaaagg cataatggtt tagtagatat cttttaatga
     1861 gtccgcaaac tgagcaggca ggacgaggta tcttgttttg caattccggt aaggaatgac
     1921 ccaaaagttc ttttaaatga ataacaatta acgggaggcc cttatcttga gcaaattgtt
     1981 gaacggcttg aagagattgt ttggaaaagt tgggaattcc aagatctaag tggatagcag
     2041 ttatttggtt accaatatca gttaataccg accataaagc catgctatct ttcccacctg
     2101 aaactgccaa tattaccggt tctttttttg aaaacatttg atactttttt atggcttgta
     2161 tggtttgttt ttgaataaac attttaaagc atctttgaca aaacgcagca tggtgacgtt
     2221 taattcgaag aatagcttca tctttacagg cggtacattt caaacccttc acctccgata
     2281 atcgtttctt attaattttg cattaaaaca tattcaggtt gaaatttcta tcctgaaaac
     2341 atacgggcat aataaatcaa gctcctacca aagaatatag aatgtaggag cgcaataaat
     2401 tgcgcccgat cttcatataa tgtagcgacc tgccatggca tgtcgaatct tggtttttca
     2461 tcttcacctg gttcagcaaa acagcatgac cgactatcct tttaaaatag tgagcattga
     2521 tatgagagtg ctgaataata aaaattatca ccctcatcct caccctctcc cttcaaggga
     2581 gaaggtgtaa ttaaatcaaa gaaataagaa gattttcgta ggggcagacc tatgtgtctg
     2641 ccctcattat tttgagataa catcatgcaa ttcttttcct cggattggtg tagaggtggt
     2701 ttcatttttt atctttagag taattatttt ctttataaac acttgtaaaa tgggtagatg
     2761 gtgaatataa taggtactgc tggcggtgta gctcagttgg caagagcatc cggctcatac
     2821 ccggagcgtc aggggttcga atcccttcac cgccaccagc ttcctcaacg ttattcctat
     2881 caggagccct cgctggtatg aagaaatatc cttatttgtt aaaaaccgtt tcccgactca
     2941 ttcaagagca ggaattaatc gttacgggag accgagtttt aatcgctttt tctggtggtc
     3001 ccgactctac tgcattatcc gatattctgg ggaaactatg tgaagaattc aaatttcaat
     3061 tagctttttt tcatttaaat catggtttaa gaggtcaaga agctcttcgg gatcaaaatt
     3121 tctgtgttga atgggcaaaa aaacgcaatt ttgagatttt tatagaacaa tatgatgtga
     3181 aagcttacaa aaaggagcta tctatttctt tagaagaagc cgctcggaag gttcgatacc
     3241 aaaaactaaa agaagttgcc gagctatgga aagctgataa aattgctctt ggtcatcatc
     3301 gtaacgacca agttgagacc atcctgatga atatcatccg aggaactggt ataaatggcc
     3361 tcagaggaat gccaattcga aatggaaagt ttatccgtcc acttttacga acttcgcttg
     3421 atgagattca tcgctatctt gaagaacaac accttgcgtt tgtcattgac tcaactaatc
     3481 ttgatcaatc ttttttgaga aatcggatcc gtaatggact aattccctta ttaaaacagg
     3541 attacaacgc caaaattgaa gaaataattt ttcgtatggg attaaatatc caagaatctc
     3601 tatcaattat acctgaagag aagtggtcaa ttgaaaaaaa aggtcgtctt tttcgcttgc
     3661 cggtgagcag tttggttgaa acttct
//
LOCUS       DAOSOL010000135         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25275_length_3670_cov_3.302351, whole genome shotgun sequence.
ACCESSION   DAOSOL010000135 DAOSOL010000000
VERSION     DAOSOL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25275_length_3670_cov_3.302351"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PK171_04150"
     CDS             complement(<1..504)
                     /locus_tag="PK171_04150"
                     /inference="COORDINATES: protein motif:HMM:NF027971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT05414.1"
                     /translation="MRITFLGAAREVTGSCYLIEGRNKRFLIDCGMVQGSGEERNAAS
                     FSFDPGSIDFLLLTHAHLDHSGRIPFLYKDGFRGKIFATAPTIELCEVLWLDMYKIML
                     EETNRTNRKNLRAGRPSIEPLYTEEDVLGALNLFEPVGYDEIVPVDNIESVFRNAAHI
                     IGAATIEI"
     gene            complement(519..1439)
                     /locus_tag="PK171_04155"
     CDS             complement(519..1439)
                     /locus_tag="PK171_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05415.1"
                     /translation="MEVMSYGGWEKCIRMENGKIELVVTQEVGPRVIRAGFIGKQNLF
                     KEFPDQKGKVGGDEWRIYGGHRLWHAPEAIPRTYFPDNHPVAVAGNKNILVLTAETEN
                     TSGIQKKLILQLSENENRIKVTHQLFNQNLWPIEFAVWSLSVMNIGGTAIVPQEQFVP
                     HTDLLTPIRPMALWGYTNMNDPRWRWGQRYVTLQQRTDMKEPTKAGFGNKKGWVAYGV
                     NGFLFIKISSYQDGASYPDYGSSTELYTNPDICEVETLSPLKLINPGEMHEHIENWFL
                     YSDVQFKNTDESIDRNVLPMVEKSMEILKG"
     gene            complement(1512..2393)
                     /gene="galU"
                     /locus_tag="PK171_04160"
     CDS             complement(1512..2393)
                     /gene="galU"
                     /locus_tag="PK171_04160"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOT05416.1"
                     /translation="MKEKPISKAVIPVAGYGTRLLPVTKSQPKEMLPIVDKPAVQYVV
                     REAIDSGVESILFVTGRGKRAIEDYFDYAVELELELENKGKLDLLQEVRDIGDMIRIF
                     YVRQKLQKGLGDAVYQAKRFINDSPFAVLLADDIIDSTDPVLRQMIDLYRKRPGIILA
                     VTRIPQEEISQWGVIKGKEIGKGTFKVEDMIEKPDPGEAPSDLAIIGRYILTPSIFEA
                     IEKVSPGKGGEIQLTDSIRSLLKKEEIYAYEFQGTYYGVGDKIGFLKANVAYALKRKD
                     IGDELKGFLKQIIEEEK"
     gene            complement(3383..>3670)
                     /locus_tag="PK171_04165"
     CDS             complement(3383..>3670)
                     /locus_tag="PK171_04165"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOT05417.1"
                     /translation="KEFCKIQGYDLGKDVQYEVTGIRSGEKIIEKLWEDDEKVSDTAH
                     QKILKIVNNTDSLPWDDLVQFVRDLEKRILEGKPVEVKIEIMEFLKRNSEI"
BASE COUNT         1000 a          884 c          597 g         1189 t
ORIGIN      
        1 aatctcaatg gttgctgcac caataatatg tgcagcattt ctgaaaactg attcaatatt
       61 atcgactggt acaatttcat cgtatccaac tggttcaaaa aggtttaaag ctcccaaaac
      121 atcttcttcg gtatacaaag gttctattga aggccttccg gcacggagat tttttcgatt
      181 ggtccggttg gtttcttcca gcataatttt atacatatcg agccagagaa cttcacatag
      241 ctcaatggtg ggagctgtgg caaatatctt tcctcggaat ccatctttat aaagaaaagg
      301 aatccgtccc gagtgatcaa ggtgagcatg ggttaataaa agaaaatcaa tgctcccggg
      361 atcaaaggaa aaagaagcag catttctttc ttcaccactt ccctgcacca taccacagtc
      421 aattaaaaaa cgtttattcc gtccttcgat tagataacag gaaccggtta cttctcgagc
      481 tgctcctaaa aaggtaattc tcatttatac ctccccggtt atccttttaa aatttccatt
      541 gatttttcaa ccattggtaa tacgttacga tcaatcgatt catcggtatt tttaaactgc
      601 acatcgctat ataagaacca gttttcaata tgttcatgca tttctcctgg atttattaac
      661 ttaagtgggc tgagagtttc tacctcacag atgtccggat tggtataaag ctcggttgac
      721 gacccgtaat cagggtatga tgctccatct tgatagctac ttatcttaat gaataaaaaa
      781 ccgttgactc cataggctac ccaacccttt ttattcccaa atccagcttt ggttggttct
      841 ttcatatccg ttctttgctg gagagtcaca tatctttgcc cccaccgcca acgcggatca
      901 ttcatgttgg tatatcccca taaagccatc ggtcggattg gagtcaataa atcagtatga
      961 ggaacaaatt gttcttgggg tactattgcc gtgcccccaa tgttcataac tgataatgac
     1021 cagacggcaa actcaatcgg ccagagattt tgattaaaaa gctgatgagt taccttgatt
     1081 cgattttcat tttccgataa ctgtaaaatc aacttttttt gaatccccga agtattttca
     1141 gtctcagcag ttaaaactaa aatattttta ttcccagcaa ctgccactgg atgattgtca
     1201 gggaaataag ttcgtggtat agcttctggt gcatgccaaa gacgatgtcc cccataaatt
     1261 ctccattcgt ctccaccaac tttgcccttt tgatcaggaa attctttaaa taaattttgc
     1321 ttcccaatga aaccggctcg aattactcga ggaccgactt cctgagtaac cactaattca
     1381 attttgccat tttccattct tatacacttt tcccaaccac cataactcat gacttccatc
     1441 gattcctaca cctctctcaa gaactaaata tctatagtat acaactatcc attcaaaatc
     1501 gtgagttcac cctatttttc ttcttcgata atctgcttta aaaatccttt caactcatcg
     1561 ccaatatctt tccgttttaa ggcataggcc acatttgctt ttaaaaaccc aatcttatcc
     1621 cccactccat aatacgttcc ttgaaattca taagcgtaaa tttcctcttt ttttaagagg
     1681 cttcgaatcg aatcagtgag ttggatttct cctccttttc ctggggatac cttctcaatc
     1741 gcctcgaata ttgacggggt taaaatatac ctgccaataa ttgctaaatc ggaaggggct
     1801 tcgccaggat cgggtttttc aatcatatct tcaaccttaa atgttccctt tcctatttcc
     1861 ttccccttaa tgacgcccca ttgcgaaatc tcctcttgag gaatccgggt cactgccaaa
     1921 attatccctg gtcttttacg atataaatcg atcatttgtc gaagcaccgg atcagtggaa
     1981 tcaatgatat catcagctaa caaaactgca aaaggtgaat cattaataaa gcgttttgct
     2041 tgataaacgg catctcctaa gcctttttgc agcttctgac gtacataaaa tattcgaatc
     2101 atgtcgccaa tatcacgaac ttcctgcaag agatcaagtt ttcctttatt ttccaattcc
     2161 aattccaatt ccacggcgta atcaaaataa tcttcaatgg ctcttttccc tcggccggta
     2221 acaaaaagaa tactttctac tcccgaatcg atggcttctc tcaccacata ttgaaccgct
     2281 ggtttatcga caatcggtag catttctttc ggctgagact tggttaccgg taataaacgg
     2341 gttccatatc cagccaccgg gatcactgcc tttgatattg gtttttcttt catcggccac
     2401 actcctaacc agtttttcta ttctgtaaat ctattagcgg gcatgataaa tcaagcccta
     2461 caaaaaatcc attttctttc tttcctctcc cctggtggga gaggattaag gtgagggggc
     2521 ctttgcactt tcctcaatct ttatctcaaa gcactaagct tggatctaca tcctgggctt
     2581 tcaccctcat cctgaccttc tcccatcaag ggagaaggaa acaaaacgat gcgttatcct
     2641 gagaattcgc ttttcgaatc cgtgaggatc tcatctgaca ccgcagacgt catcctgagc
     2701 ggtgctttcc cgcgtgagga tctcatcttt ttaatttttc ttttatcctt taattctttt
     2761 ttcattctcc ccctcgcccc ctcgtctttt aaaaatcccc ctcgccccct cgcgccctct
     2821 cctcctcggt atttaagggt gtgaggatct catctgacgc tacacgcgtc atcctgagcc
     2881 ctcgcttttc gaatccgtga ggatctcatc tttttatttt ttcctttttt ttataaatac
     2941 tttaaaagat gagattgcca cgtcgctgcg cttctcgcaa tttcgttaat ggtggttgag
     3001 attctcacgt cacttcgttc ctcgcaatga cggggcagga tggatgggat tctcacgtcg
     3061 tccggtaaaa gacaccagac tcctcagaat gatagagaaa atgatgctcc ctcacccctt
     3121 ctgcatttct tgcacccatt tttttataat gtagtgacat gccatggcat gtcgaatctt
     3181 gagtttcacc ctcatacctc atactactaa actatataaa aatctaatcc tcattttggt
     3241 tttttacctt caaaggtaat tttattatat caaaccagct aaaataagaa gaaaaacagc
     3301 gaggagcata aaaactgtgg accgtgaaaa gtgagccgtg aatcgaaaaa aattcgcaaa
     3361 atggagaaac gacttttatt gtctaaattt ctgaattcct ttttaaaaat tccattatct
     3421 caattttaac ttcaaccggt ttcccttcca ggattctctt ttccaaatct cggacaaact
     3481 gtaccaaatc atcccatggt aaggaatcag tgttattaac aattttcaaa attttctgat
     3541 gggcagtatc cgaaaccttt tcatcatctt cccaaagttt ttcaatgatc ttttctccgg
     3601 aacggattcc ggttacttcg tattgaacgt cttttcctaa atcatatccc tgtattttac
     3661 aaaattcttt
//
LOCUS       DAOSOL010000136         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25348_length_3661_cov_3.844426, whole genome shotgun sequence.
ACCESSION   DAOSOL010000136 DAOSOL010000000
VERSION     DAOSOL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25348_length_3661_cov_3.844426"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PK171_04170"
     CDS             complement(<1..302)
                     /locus_tag="PK171_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05418.1"
                     /translation="MDTDWKDRIEKANALWERVNLLQEEIELIEKQLNEILQIEEEEI
                     LEEDSQSIEKKIFELFSQVLAELKQQEENILKVPPERWKKIQTREVLDLEKMSLDS"
     gene            complement(306..1310)
                     /gene="trpS"
                     /locus_tag="PK171_04175"
     CDS             complement(306..1310)
                     /gene="trpS"
                     /locus_tag="PK171_04175"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT05419.1"
                     /translation="MGNKDRIFSGMRPTGKMHIGHYLGALKNWLQLQKEYDCIFGIVD
                     WHALTTSFEHTEDIERNIIDMAIDWFSVGIDPEVCIPMIQSLVPEHAELHLLLSMVTP
                     LGWLERNPVLKQQLQDMGLKEAVGYGHLGYPVLMAADIIIYQGVKVPVGEDQVPHIEI
                     AREMVRRFHYLYQQEVFKEPQPLLTETPRILGIDGKKMSKSLNNAIGLSDTKEELRTK
                     VLSMFTDPEKIRKRDPGHPERCNVFSFQNIVGNPRIEQIEKDCRTGDLGCVDCKKELL
                     GLMVDYLEKISPFRGRYQGKEQLVKDILVEGSRKAQTLARETMKKVREAMNIDYQIPR
                     "
     gene            complement(1294..>3661)
                     /locus_tag="PK171_04180"
     CDS             complement(1294..>3661)
                     /locus_tag="PK171_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT05420.1"
                     /translation="EELIQAGRVAFAIYDHHPETPDGLKGEQVVVQPTGACTTILVEL
                     IKEQTLPLNSLEATLLILGIYEDTGSLTFPTTTARDLQAAAFLVEHGAKLSAIPDYVN
                     MQLTNEQRDVLKLMVNSLTVVEVNGIQVHFTSVQVKDYVDGVSFLVHKLMDLEEINVL
                     FSLFDMENKVYVIARSRLEEIDVSIITTGLNGGGHKSAASASIKGSSLEEVRKKIINM
                     MKRSFSFTRAADIMSTPVKTVHRESSINDAYLTLIRSGFSGLPVVDDEGMLVGIIARR
                     DMEKAIHHGLQNAPIKSFMSPQIIGVSPRDSIYTIRNLMVENDIGRVPVIKAGKPIGI
                     ITRSDLLRVFHQKENIVVNELTRLNVSESVFAHFIAADLELINLISGFARRVGVRTYL
                     VGGVVRDIILGVPNHDLDLVVEGDAIEFSQNLNRLLNGKVVAYPPFGTSVVFMKDKKR
                     IDFASARKEFYRFPGAAPDVEYSDLKKDLFRRDFTLNAMAISIYPDNWGELYDFFGGY
                     RDLRSKILRILHPLSFVDDPARSIRAVRFEKKYGFHIEPFTMSLLKQTIRKNLLDSIK
                     PDRLKEEIQLILELPNYYRYLERLYQLDMIPSILPGCIWKREYSDRFIDVEKRLPEVK
                     TILGIDRFLYQLTPLFDDFDIKQISLLQKRLALSRHFTHKVVSYHSKKSEFRWAIEEK
                     DIRPSEVYLRCRSIPLEFLYYLLSFYPENSLASQRLNRYLKEWITIKTILKGSDLQEM
                     GIKAGPHYATMLDELKLAKIDGHAMNRLEEEQLIRQYWERVKKRGEQR"
BASE COUNT         1089 a          815 c          627 g         1130 t
ORIGIN      
        1 aagctgtcca gtgacatctt ttctagatca agaacttctc gagtttgaat ttttttccaa
       61 cgctcgggag gtacttttaa aatattttct tcctgttgct ttagctcggc aagaacctga
      121 gaaaacaatt caaagatctt tttctcaata gattgactat cttcctccag gatttcttct
      181 tcttcgatct ggagaatttc gttcaactgt ttttcaatta attcaatctc ttcttggagc
      241 agatttaccc tctcccaaag agcgttggct ttttctatcc gatctttcca gtcagtatcc
      301 ataatctatc gaggtatttg gtaatcaata ttcatcgctt ctcgaacttt tttcattgtt
      361 tcacgagcaa gagtctgagc tttccgactc ccttcaacta aaatatcttt gactaactgt
      421 tctttccctt gatagcgtcc ccggaaagga gaaatctttt ccagataatc aaccatcaat
      481 cccaatagtt cttttttgca atcaacacac cccagatctc ctgttcggca gtctttttca
      541 atctgttcga ttcgaggatt cccaacaata ttctgaaaag aaaagacatt acatctttcc
      601 ggatgtcctg gatcacgttt tcgaattttc tctggatcgg taaacattga aagaactttg
      661 gttcgtaatt cttctttggt atcagataga ccaatagcat tatttaaact cttgctcatc
      721 tttttcccat caattcccaa gattcgtgga gtttcagtaa gaagcggttg tggctccttg
      781 aacacttctt gttgatagag ataatgaaaa cggcgtacca tttctcgggc aatctcaata
      841 tgaggaactt gatcttctcc aaccggcacc ttaacccctt gataaataat gatgtcagca
      901 gccattaaaa ccggatagcc aagatgccca taaccaactg cttctttcaa acccatgtct
      961 tgaagttgct gctttaaaac cggatttcgc tccagccacc ccaagggagt gaccatggaa
     1021 agaagaagat gaagttcggc atgttccgga accaacgatt gaatcatagg aatacacact
     1081 tcaggatcaa tacccacact aaaccaatca atagccatat caatgatatt tcgctctata
     1141 tcctcagtat gttcaaaaga ggtggtcaaa gcatgccaat cgacaatacc aaaaatacag
     1201 tcatattctt tttggagctg caaccaattt tttaatgctc ccagataatg accgatatgc
     1261 attttaccgg ttggtctcat tccgctaaaa atcctatctt tgttccccac gttttttcac
     1321 cctttcccaa tattgcctaa tcaattgttc ttcttctaaa cggttcattg cgtgtccatc
     1381 aatttttgcc aattttagct catccagcat tgtagcatag tgaggaccag ctttaatgcc
     1441 catttcttgc aaatctgagc cttttaaaat ggttttaatg gttatccatt ctttaagata
     1501 ccggtttaat cgctgacttg ccaagctatt ctctggataa aaactgagca aataatataa
     1561 aaactcaagg ggtatcgatc gacagcgaag ataaacctct gagggacgaa tatctttttc
     1621 ttcgatagcc cacctaaatt cacttttttt ggagtgataa ctcactactt tatgagtgaa
     1681 gtgacgagat aatgctaaac gcttttgcaa taatgaaatt tgcttgatgt caaaatcatc
     1741 gaatagagga gtcagttgat aaagaaacct atcaattcct aaaatggttt ttacttctgg
     1801 cagtcttttt tcaacatcaa taaaccgatc ggaatattcc cttttccaga tacaacctgg
     1861 taaaatcgac ggtatcatat ccaattggta aaggcgttct aaataacgat aataattagg
     1921 tagttccagt atgagctgaa tttcttcttt aagacgatcg ggcttaatac tatctaataa
     1981 attttttcga atagtttgtt ttaagagact catggtaaac ggttcgatat gaaaaccata
     2041 ttttttttcg aaccggaccg ctcgtatgga tcgagcggga tcatcaacaa agcttaatgg
     2101 atgaagaatt cgaagaatct tacttcttag atctcgatag ccaccaaaaa aatcatagag
     2161 ctctccccaa ttgtcaggat aaatactaat ggccattgca tttaaagtaa aatctcttcg
     2221 aaataaatct ttttttaagt cagaatattc cacatctggt gctgcacctg gaaaacgata
     2281 aaattctttt cgagcactgg cgaaatcgat tcgtttctta tctttcataa aaacgaccga
     2341 tgtgccaaag ggtggatagg caactacctt cccattaagc agccggttga gattctggga
     2401 aaattcaatt gcatctcctt ctaccaccaa atctaagtca tgattaggaa cacctaaaat
     2461 tatatccctt accactcctc caactaaata agttcttact ccaactcggc gggcaaagcc
     2521 actaatcagg ttgatcagtt ccaaatcagc agcaataaag tgggcaaata ccgactcaga
     2581 aacatttagt cgggtcaact cattgactac aatattttct ttctgatgaa acacccgcaa
     2641 aagatcactt cgggtaatta tcccgattgg tttccctgct ttgataaccg gtactcgtcc
     2701 tatatcattt tctaccatta aatttcgtat agtatatatc gagtcacggg gcgagactcc
     2761 aatgatctga ggtgacataa aactcttgat cggggcattt tgtagaccat ggtggatggc
     2821 tttttccata tctctccggg caataattcc caccaacatc ccctcgtcat cgaccaccgg
     2881 caatccggaa aaacccgaac gaattaaagt taaataagca tcattgatac tgctttcacg
     2941 gtgaacagtt ttcactggtg ttgacataat atcagcagcg cgagtaaaag agaatgagcg
     3001 tttcatcata ttgatgattt tttttctgac ttcttccaaa gaacttccct tgatcgaagc
     3061 cgatgctgca cttttatgtc ccccaccatt caagccggta gtaatgatgc ttacatcgat
     3121 ttcttccagc cggctacgag caataacgta caccttattt tccatatcaa aaagtgaaaa
     3181 aaggacatta atttcttcca aatccattaa tttatgaacc aaaaaagata ccccatcgac
     3241 ataatcctta acctgaactg aagtaaaatg aacctgaatc ccatttactt caaccacggt
     3301 tagggagttc accataagtt ttaaaacatc ccgttgctca ttagtaagtt gcatattgac
     3361 ataatcaggt atggcactca actttgctcc atgttcgact aaaaaggcag cagcttgaag
     3421 gtcacgggcg gttgtggttg gaaaggttaa tgagccggta tcttcatata ttccaagaat
     3481 taaaagagtc gcttccaagg aattaagagg aagagtctgt tctttgatca gctccaccaa
     3541 aatggtagta caggcaccgg ttggctgaac aaccacctgt tcccctttta atccatctgg
     3601 tgtttcggga tgatgatcat aaatggcaaa agctaccctc ccagcctgaa tgagctcttc
     3661 g
//
LOCUS       DAOSOL010000137         3652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25423_length_3652_cov_4.711148, whole genome shotgun sequence.
ACCESSION   DAOSOL010000137 DAOSOL010000000
VERSION     DAOSOL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25423_length_3652_cov_4.711148"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PK171_04185"
     CDS             complement(<1..539)
                     /locus_tag="PK171_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT05421.1"
                     /translation="MTEKITFKQFPLRQEILCALNKKKFDNPSPVQVQVMNETNIMNE
                     DLIVQAKTGSGKTLAFGIPLLNTFEKIPTQPAVLILSPTRELAIQTAHELKWLGSEMD
                     IKIATLVGGMDIVNQRRELLNGPALVVGTPGRVLDHIRRGHYRTEEIQTIVLDEGDHM
                     LDLGFREELEAIFESHEKV"
     gene            complement(1086..2135)
                     /gene="mtnA"
                     /locus_tag="PK171_04190"
     CDS             complement(1086..2135)
                     /gene="mtnA"
                     /locus_tag="PK171_04190"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOT05422.1"
                     /translation="MFKTIEWKNFQVRFLDQRQLPLEEKFIETNDFRVVADAISNLGL
                     RGAPLIGVGAAYGMALGAQEGLNTHSDQYFSFLENVDQTLRSTRPTAVNLFWALDRMK
                     KIWKNAEKQGVSPSNCTKKLVEEAITIHDEDIETNQNIAKQGSSLIEDQDAILTHCNA
                     GALACSAWGTALGVIYWAALQDGKKLMVYADETRPLLQGARLTSFELMKNNIPVTLIT
                     DNMAGYLMAQGWVHKIIVGADRIAANGDTANKIGTYSLAVLASYHKIPFYIAAPLSTI
                     DIHIATGDEIPIEERKPEEIFLFGESRTAPQGVGAWNPAFDVTPHQLIRAIITEKGIL
                     YPPFAEKLSLLKQME"
     gene            complement(2696..3157)
                     /locus_tag="PK171_04195"
     CDS             complement(2696..3157)
                     /locus_tag="PK171_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOT05423.1"
                     /translation="MNSQLWIALLSFSPISEIRGSLLYWASNPVIPFWQALLISILFN
                     LLPFFVVMFFLNAILSIFLRFQWFQSLWDRLVVSTRKKFQKYEKWEQIGLAFFIGVPL
                     PITGVWTGSLICFLMGWPLKRSFPFVLLGLGIASTIVSVIVLSGRRVLTLF"
     gene            complement(3154..>3652)
                     /locus_tag="PK171_04200"
     CDS             complement(3154..>3652)
                     /locus_tag="PK171_04200"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT05424.1"
                     /translation="SNAAGGMNRNMKRSDLMIICDHINLLGDNPLRGENDPELGPRFL
                     DMSNAYDPDLIQLAQQIALEKGIYVMKGVYAALAGPNFETPAEYRFLISIGADAVGMS
                     TVPEVLVARHSGMKVFGVSCITDLAIDGFIEPVSHEVVIQAANEAQPKLKLLIRELVG
                     KINFA"
BASE COUNT         1092 a          761 c          700 g         1099 t
ORIGIN      
        1 tcaacttttt catgtgattc aaaaattgct tccaactctt cgcgaaatcc taaatctaac
       61 atatggtcgc cttcatcaag aacaatggtc tggatttctt cagtacgata gtgacctctc
      121 cgaatatggt ctaaaactcg tccaggtgtt ccaactacca aagcgggtcc atttaataat
      181 tctcggcgtt gatttacgat atccattcca ccaaccaaag ttgctatctt tatatccatc
      241 tcgctcccta accatttcag ctcatgagct gtctgaatgg ctagttccct tgttggagac
      301 agaattaaaa ccgccggctg agttggaatc ttttcaaatg tattgaggag aggaattccg
      361 aaagccaagg tttttcctga tccggtttta gcttggacaa tgagatcttc attcataata
      421 ttggtttcat tcatcacttg aacctgaacc ggactcggat tatcgaattt tttcttattt
      481 aaagcacaga gaatttcctg acgaagcgga aattgtttaa aagttatttt ttcggtcaaa
      541 gtattgacct cctttttttt aaaatcgaaa agatcgtgat caaaaccact cattgacatt
      601 ttttccgatg agatccgatc catcgatttg aggaggtaca cccattggac attaaatgcc
      661 accatttgtt cccaatatgt agttataact ttgtttgaaa tgacgatcca ttcgctcttt
      721 ttgagactca aaaaagggga cttgacggaa gaactggaag tgtaaggccg gttacttcat
      781 caaaaccttc gtgattctcg agggaacatt ataacagata ctaaaacaaa tgaatatctt
      841 ttttatttct tttaagaata attcaatata aaagtctact tttccatttt gaagagaaag
      901 ctttggtaga acaaaaattc atgctaattt cgtaatacca gatggatatg aaaaataaga
      961 ttcaacctgc catggcatgt cgctacatca attaaagtgc gggcgtgata aagaccgagg
     1021 gggagagggg gcgaggagct tgatttatca agcccatggg tattcagaag agatttatat
     1081 caattctact ccatttgctt caaaaggcta agtttttcag caaatggagg ataaagaatg
     1141 cccttctccg taatgatagc ccgaatcagt tgatgtggtg tcacatcaaa agccggattc
     1201 cacgctccaa ccccttgggg cgccgttcga ctttctccaa aaagaaatat ttcttctggc
     1261 tttctctctt caataggtat ttcatcccca gtggcaatat gtatatcgat agttgaaagc
     1321 ggtgcggcaa tataaaaagg tatcttatga taggacgcca gaactgctaa ggaataggtt
     1381 ccgattttat tggctgtatc cccattggcc gcaatgcgat ctgctcccac gatgatttta
     1441 tgaacccatc cttgcgccat aagataaccg gccatattat cagtaataag tgttactgga
     1501 atattatttt tcatgagctc aaaactagtg agacgggctc cttgaaggag tggtcgggtt
     1561 tcatcggcat aaaccattag tttttttccg tcttgaagag ctgcccagta tatcactcct
     1621 aacgctgtcc cccaggcact acaggctaat gccccggcat tacaatgggt caatattgca
     1681 tcttggtctt caatgagaga tgaaccctgt ttagcaatat tttgattggt ctcaatatct
     1741 tcatcatgaa ttgtaatagc ttcttctaca agtttttttg tgcaattact tggtgataca
     1801 ccttgttttt ctgcattttt ccaaatcttt ttcattcggt ccaaagccca gaataaatta
     1861 accgcggttg gtcgagttga tcgtaaagtt tggtctacat tttctaaaaa ggaaaaatat
     1921 tgatccgaat gggtatttaa accttcttgg gcgcccaatg ccataccata agctgccccg
     1981 acaccaatca ggggagcgcc tctcaagcca agatttgata tggcatcagc gacaacacga
     2041 aaatcattgg tttcaatgaa tttttcttct aaagggagtt gcctttgatc gagaaatcta
     2101 acttgaaaat tcttccattc aatagttttg aacattttct aatctccgta agcaaatatt
     2161 ttaattttgt taattatttg tgaatttttt tctgtttcaa atgtttttag gatacatctt
     2221 cgtaccattt ggtggcgcca gattaggatg aaaaaaggtt acccctcctc atcctaaccc
     2281 tctcccacga aggggcgagg ggaaaaagct ctccctttag aaaagggggt aagggggaat
     2341 ttgattttta ctgttccgtc attgcgagga acaaagtgac atggcaatct ctgccacctc
     2401 atatgtcatt ctgatgagtc cggtgttttc taccggacga cgtgaggatc tcatcttttt
     2461 attctttttt ctaggtgatt ttctcaagat atcaaagaaa attcgaaagg caccctcccc
     2521 accgtaaaac ggcacccctc caaggagggg aattgttgaa ttcattcccc cattgagggg
     2581 ggattcaggg gggtgtgcct ttttcttttt ttgtttattc ttttttcacc aatattggta
     2641 ttttcaggat agaccatagt cctaactagc gttgctaaaa ccataaccct atatattaaa
     2701 aaagagtcaa tactcttcgt cctgaaagca caatcacgct cacgatagtc gaggcaattc
     2761 ctaaacctaa taaaacaaag ggaaaggatc tttttaatgg ccatcccatt aaaaagcata
     2821 tcaaagagcc cgtccatacc ccggtaatag gaagtggaac tccgataaaa aaagccaagc
     2881 cgatctgctc ccatttctca tatttctgaa atttcttccg agtgctgacc accagtctgt
     2941 cccagagtga ttgaaaccat tgaaatcgta aaaatatcga aaggatagca ttaagaaaaa
     3001 acatcactac aaaaaatgga agaagattga ataaaataga aataagaaga gcttgccaga
     3061 aaggaataac cggattagat gcccaataca gtaaagatcc tctaatttct gaaattggag
     3121 aaaaactcaa aagagcaatc catagttgtg aattcatgcg aagtttattt tgcccactaa
     3181 ttcccgaatg agaagtttaa gttttggttg agcttcatta gccgcctgga tgacgacttc
     3241 atgagatacc ggttctataa acccatcgat tgctagatcg gttatacaag atactccaaa
     3301 aactttcatg ccggaatgac gagctacgag aacttcgggc accgttgaca ttccaaccgc
     3361 atcagcacca attgagatca aaaaacgata ttcagcagga gtttcaaagt ttggtccagc
     3421 aagagcagcg tagacacctt tcataacata tatccccttt tcaagggcga tttgttgagc
     3481 caattgaata agatcagggt cataggcatt tgacatatcc aaaaatcgtg gtcccaactc
     3541 cgggtcattt tcaccacgaa gtggattgtc acccaaaagg ttaatatgat cacaaataat
     3601 cattaaatcg ctgcgtttca tgtttcgatt cattccacca gcggcattgc tg
//
LOCUS       DAOSOL010000138         3633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25618_length_3633_cov_3.543600, whole genome shotgun sequence.
ACCESSION   DAOSOL010000138 DAOSOL010000000
VERSION     DAOSOL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25618_length_3633_cov_3.543600"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..1177
                     /locus_tag="PK171_04205"
     CDS             62..1177
                     /locus_tag="PK171_04205"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOT05425.1"
                     /translation="MLEEEFSGKIGIKHSLAVNSCTSALITALVAVGVGPGDEVIVPG
                     YTFFASCAAILAAKAIPVIAEVDNSLTIDPIDLEKKITPQAKAVIPVHMRGVPCNMDR
                     ILSIAKKHNLKVIEDVAQACGGSYKGKYLGSLGDCNTFSFQFHKIITAGEGGMITTDS
                     DLYFDRAQAYHDTAACWRPGGPAKRFVSARYQGELFPGVNFRMSELIGAVMLAQLHKL
                     DGILNRMRKNKSRIKNGISDIPGIELRHLHDPAGDTGISLVFFLENGQLAEKFASALK
                     AEGVEASSIFNKGIPDWHIYSHWEMIMNKWTPTSEGCPYTCPYHKGSVPNYNREMCPQ
                     TLALLDRSVHLDIPPQMSEEDCDLTVEAIRKVAGAYL"
     gene            1223..2479
                     /locus_tag="PK171_04210"
     CDS             1223..2479
                     /locus_tag="PK171_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOT05426.1"
                     /translation="MKHRDMKLLNREKILGLLLNHSLLSRAELAQKAAVSPAVVSHII
                     RECLKEGIVCEDSEGVSRGGRKPILLSFVPDNKLVAGLVPGNTTEFAVSNLSGQLLYK
                     EDIATKKYPTPSELAVFLKKILHEFLIRNHKSWSQLVSLGIGIPGIYDPELDLIHKMG
                     KISSLFEWEGTKIREIFSREFLCPVLVFDKVVAMAFGEGILSSEDPNVNLVYLHLGRG
                     VGAGLFLNGKIYRGSQGAAGEVGYMVCGPVDEKGPISLHDIPPLESEISLNSILKVFR
                     EKCPNSFPNSLRQSENDEVLILHKTFLKGDSQCEAILKPILEKAEGIAVNLVAVLNPD
                     LLVLGGKVTEIFSEILQERIIQRLQRSVLFSPQVKTVTLGKNEEIMCTLFFAIDDYFR
                     RLTGNNQGLTSAFLKRYSPSSRINKL"
     gene            2564..3331
                     /locus_tag="PK171_04215"
     CDS             2564..3331
                     /locus_tag="PK171_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOT05427.1"
                     /translation="MVEISVCFEPLFNRKTDLEKIQTIHSLGYNAVEFWDLAGKDLKA
                     ITAYCQKTGLKIGICTLKDPWGNIRLHADTNSYLQHFSESLELLKQVGSKRVIVLTGE
                     DDGQKKEVQVNQIIKNLKAISSLAEKAGIVVCLEVLNSKVDHKGYFLDSTALGFEIIR
                     QVNSPSIKILYDIYHMQIMEGNIISTIQENIELIGHFHSAGVPGRHEHFYGELDYRNI
                     FMAIQETDYNGYFGLEYWPSLSDEKSLEKLREYFLDE"
BASE COUNT         1097 a          728 c          745 g         1063 t
ORIGIN      
        1 ttctggataa taatatcttt tccgttatta cggaccagga gaatacccat ctcgagtcaa
       61 aatgctcgaa gaagagtttt ctggtaaaat aggaatcaaa cactcattag cggtaaattc
      121 ttgcacctca gctttaatca ccgctcttgt agcagtcggt gttggtcctg gtgatgaagt
      181 aatcgtcccg ggatatactt tctttgcctc ctgtgcggca atattagcag caaaagcaat
      241 tccggttatt gctgaagtag acaattcact tactattgat ccaatagatt tggaaaagaa
      301 aattacacca caagccaagg cagtaatccc cgtacacatg cgcggtgttc cctgcaatat
      361 ggatcgaatc ctctcaatcg caaaaaaaca caaccttaaa gtcattgaag atgttgccca
      421 agcttgtgga ggaagctata agggtaagta tttagggagc cttggcgatt gtaacacctt
      481 tagtttccag tttcataaga ttatcactgc cggtgaaggt ggcatgatta caaccgatag
      541 tgacctctac ttcgatcgtg cccaagccta ccacgatact gctgcttgct ggcgaccggg
      601 tggcccagcc aagcgattcg tatcggctcg ttaccaggga gagctcttcc ctggagtcaa
      661 tttccgaatg tccgaactca tcggagcggt catgcttgcc caacttcaca aacttgacgg
      721 cattctcaac agaatgcgta aaaacaaatc cagaataaaa aatgggatca gtgatatccc
      781 aggaatcgag ttgagacacc ttcatgaccc ggctggagat actgggattt ctttggtttt
      841 ctttttggaa aacggacaat tagccgaaaa gtttgcctct gccttaaagg ctgaaggtgt
      901 agaagccagt tcaattttta ataaaggaat acccgactgg cacatatatt ctcactggga
      961 aatgatcatg aataaatgga ctccaacctc cgaaggttgt ccctatacct gcccctacca
     1021 taaaggatca gttcccaact acaaccgaga gatgtgtccc caaaccttgg ctctattgga
     1081 tcgttcagtt catcttgaca ttccgcctca aatgagtgaa gaagattgcg acctcaccgt
     1141 agaagcaatt cgaaaagttg ctggtgccta cttatgatat tttatactat ttttccttaa
     1201 ttcatttttg tgaggcaatc ctttgaaaca tcgtgacatg aagcttttaa accgggaaaa
     1261 aatcctaggc ttattattaa accattcgtt actgagccgg gctgagctcg ctcaaaaggc
     1321 tgcagttagc ccggcagttg tttctcatat tattagagaa tgtttaaaag aaggaatagt
     1381 ttgtgaggat tctgagggtg tctctcgagg cggtcgaaaa ccaatccttc tttcatttgt
     1441 tcccgataac aaattggtag caggtttagt tcccgggaat accaccgaat ttgccgtatc
     1501 gaatctttcc ggtcaactgc tttacaaaga agatatagct acaaaaaaat accctactcc
     1561 ttcagagtta gcggtatttc tcaaaaaaat actccatgaa tttctaatca gaaaccataa
     1621 gtcttggtca caactggttt ccttgggaat tggaatcccg ggtatttacg atcccgaatt
     1681 ggacctgatc cataaaatgg gtaaaatttc cagtcttttt gaatgggagg gaacaaaaat
     1741 tcgggaaatt ttctctcggg aatttctttg tccggtgctg gtctttgaca aggtagtcgc
     1801 tatggcattt ggtgaaggga tactgagttc cgaagatcca aatgtcaatt tagtctatct
     1861 gcatttggga agaggagttg gagctggttt gttccttaat ggaaaaatct atcggggatc
     1921 acaaggagcg gctggagaag tcggctatat ggtttgtgga ccagttgatg aaaaaggccc
     1981 catttctctt catgatattc ctccccttga aagcgaaatc tctttaaata gcattcttaa
     2041 ggtttttcga gaaaaatgtc ccaactcttt tcccaatagt ctaaggcaat cagaaaatga
     2101 tgaagtttta attttgcaca aaactttttt aaagggcgat tctcaatgtg aagcaatttt
     2161 aaaaccgatt ttagaaaaag ctgaggggat agcagttaat ttggttgcgg ttctaaaccc
     2221 agacctttta gttctcggag gcaaggtaac cgaaatattt tctgaaattc ttcaagaaag
     2281 gataattcaa agacttcaac gatcagttct tttctcgcct caggtgaaaa ccgttactct
     2341 gggaaaaaat gaggaaatca tgtgtacact tttttttgct atcgatgatt acttccgacg
     2401 gttaacagga aataatcaag ggttaacctc cgccttttta aaaagataca gccccagctc
     2461 tagaatcaat aaattataaa agtggtcttt ttttggggtg aggaaattct taagtttccc
     2521 tcacccctta atcaaatcaa ctcatcttcg aggaggaatt aatatggtgg agatttcagt
     2581 ctgttttgaa ccgctattta acaggaaaac tgatttagaa aaaatacaaa ccatccatag
     2641 tctcggctac aatgcggtag aattttggga tttagccggt aaagatctaa aagcaatcac
     2701 cgcttattgt caaaaaaccg gtcttaaaat tggtatctgt accctgaagg atccctgggg
     2761 aaatatccgt ttacacgccg acaccaattc ctacctgcaa cacttttcag aatctctcga
     2821 attactgaag caggttggtt ccaaaagagt tattgttctt actggagaag atgatggtca
     2881 aaagaaagaa gtacaagtga atcaaatcat taaaaattta aaagcgatca gtagtctagc
     2941 ggaaaaagct ggaattgtag tctgcttaga agtattgaat tcaaaagttg accataaagg
     3001 gtatttctta gattctacag cactcggttt tgaaatcatt cgacaggtga atagtccctc
     3061 aattaaaatt ctctatgata tttaccatat gcaaatcatg gaaggaaaca tcatctcaac
     3121 cattcaagaa aatattgagc taattggtca ctttcattcc gctggtgtac caggacgtca
     3181 tgagcatttt tatggtgaat tagactatcg aaatatattt atggccattc aagaaactga
     3241 ttacaatggc tactttggtc ttgaatactg gccatctctt tctgatgaaa aatcattaga
     3301 aaaactgcgg gaatacttcc ttgatgaata atcggaaatc atttaaggta aaatatgaat
     3361 taacgttcaa gcagctttca tcaccagatg aggatgaaaa gaattccccc ttcaccctga
     3421 ccctcttcac gaagggggcg agggaaaaag aatttcactt ttaagaaagt gttttagggg
     3481 atttaatttt tccatgttcc gtcattgcga ggaacgtcct atgcgacgtg gcaatctcat
     3541 ttactgagtc tgtcattctg aggagtccgg tgtttttgcc ggacgacgtg agaatctcat
     3601 cctttccaat tctatgattc tacaatacta caa
//
LOCUS       DAOSOL010000139         3619 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_25759_length_3619_cov_3.365881, whole genome shotgun sequence.
ACCESSION   DAOSOL010000139 DAOSOL010000000
VERSION     DAOSOL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25759_length_3619_cov_3.365881"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PK171_04220"
     CDS             complement(<1..1029)
                     /locus_tag="PK171_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05428.1"
                     /translation="MNKKSFQNRIAFGVWLNDFRSTPILDDAWPSVLIDQQTINDFQK
                     TMEFLKRAGYTDIDLFGLLTNRDWPMDIESVLTPERKSQVDRLIHIIHSNGLQLIYGL
                     GVYSWGFDTIIQNDPEVRGTNPSALCGSQVKSRELMEKVIDFIGKNFIVDGYHLEAAD
                     QGRCCCEKCIQEGDVDYYNRINHLTAAYIRNRWPEKKLLVNTSGYLPWGDWMNEKERG
                     SVLDLGKNIDIFIDGGNHGQFIHENDRAVFIDRLPCDYGTSGGFWIYPPQRFDRNRWF
                     LPYILRSTTHLRNLYRDGSRSCELYLGPLINPSTEMNIFCLGRFLQDVDQNPLKIIEE
                     GVEELYHIK"
     gene            complement(1074..2024)
                     /locus_tag="PK171_04225"
     CDS             complement(1074..2024)
                     /locus_tag="PK171_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05429.1"
                     /translation="MKIKLSSGFLQKMVIFGVLLLIIIVLSFLSKSFLTVTNLNNVTR
                     QVAFVVITGCAVTPLMISGNFDLSIGSVLGLTGMLSALFVTQSIPLWFSIILSTLVGS
                     AIGLLNGLMVIKLRIPSIIATLGTMYAARGLALVISGGNSIHMGLGKNFTLLGRGYLG
                     GIPIIFIIMFLTIFLFYFIESKTILGRYTFAIGGNKRTALLSGINVGSIIILLYFLVG
                     TLTGFSGTLMASRLGVGQAMVGSGFEFDVIVAVVLGGTSIEGGEGSIFGMLIGAFIVG
                     FIANGLNLMGIHSFYQSIVKGVVLVGAVLLDQKLKGRIAD"
     gene            complement(2014..3516)
                     /locus_tag="PK171_04230"
     CDS             complement(2014..3516)
                     /locus_tag="PK171_04230"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05430.1"
                     /translation="MEKNILEVRGLAKNYPGVAALKGIDLDIRRNTVHCIVGENGAGK
                     STFIKILTCAETRSSGEILFNGKEFKPRSIREAMDLGISTLFQELNVVNQLTVEENLI
                     LGREPNRYGVIQRSDPSEKVFQLMKEFAPDIPLKKKVAQLSFAEKQVIEIVKAIAVDA
                     SLLIMDEPTAAVSENETKRLFAIIRSLKEKNITVIYISHILDDIFTIGDVVTVFRDGE
                     IVETKNVADIDRDELIRMMIGKVTVDKYVPRPVDYQKKVLEVYNLTTHKLKNVNFNLF
                     KGEILGFYGLRGAGKTEIALALFGLDRVLSGDIRIEGKSVLFDLPRDAMLSGISMVPE
                     ERLTEGLIMKLPVRPNISITNLKVLSHFGVVRSQEDREVARNYVDAMNIKVKHIEQKV
                     ATLSGGNQQKVVVSKYLHAGSNILMMDEPTRGVDIGAKEEIHRIIRNLAEKGKSIIVF
                     SSEYPEIFNLCDRIYLLRDGQIVKEIKNQEADPEEILYIITRGKRTNYEN"
BASE COUNT         1134 a          847 c          589 g         1049 t
ORIGIN      
        1 tttaatatgg tataactctt cgaccccttc ctcaataatt ttgagaggat tttgatcgac
       61 atcctgaaga aaacgtccta aacagaagat attcatctcg gtactgggat tgatcagagg
      121 tcccaaatac aattcacagg atcgacttcc atctcgatat agattgcgaa gatgagtggt
      181 tgatcgcaaa atatagggaa gaaaccagcg gtttcgatca aagcgttggg gtggataaat
      241 ccaaaaccca cccgatgtgc catagtcaca tggtaatcta tcaataaaaa ccgctcgatc
      301 attttcatga ataaactgtc catgattacc gccatcgata aaaatatcaa tattcttccc
      361 taaatccaaa accgatccgc gttctttctc attcatccaa tctccccaag gaagataccc
      421 ggaagtattc accagcaatt ttttctcagg ccaacggttt cgaatataag cagcagtcag
      481 atggtttatg cggttatagt aatcaacatc accttcctga atgcattttt cacagcaaca
      541 acgaccttga tcagcagctt ccaaatgata tccgtccaca ataaagtttt tcccgataaa
      601 atcaattact ttctccatca gttctcgaga cttaacttgc gaaccacata aagccgaagg
      661 gttggtccct cgaacttctg gatcgttttg aataatcgta tcaaaacccc agctatagac
      721 tcctaaacca taaatcagct gtaaaccatt ggaatgaatg atatgaatta ggcggtcgac
      781 ttgacttttt ctctcggggg tcaaaacgct ctcaatgtcc atcggccaat ctcgattggt
      841 aagcagacca aacaaatcga tatcggtata accggccctt tttaaaaatt ccatggtttt
      901 ttgaaaatcg ttaatggttt gttgatcaat taatacacta ggccaggcat catccaatat
      961 tggtgtactg cgaaaatcgt ttaaccaaac tccaaaagca atccggtttt gaaatgattt
     1021 tttattcata tccctaccct gtcaatactt ttcatttaaa aaccatcaat atcctaatca
     1081 gcgattcttc ccttcagttt ttgatccaaa agaaccgctc cgactaaaac cacacccttt
     1141 acaatacttt gataaaagga gtgtattccc atcaaattga gaccattggc aataaaacca
     1201 acgataaaag caccgatcaa cattccaaat atcgaccctt ctccaccttc gatactagtt
     1261 ccacctaata ccacagccac gataacatca aactcgaaac cagatccgac catggcttgg
     1321 ccaactccta agcgggaagc cattaaagta cccgaaaacc ctgtcaaagt tccaaccaga
     1381 aaataaagca agatgataat tgaaccaacg ttgattcccg aaaggagcgc agttctttta
     1441 tttccaccga tagcaaatgt ataacgacct aagatggtct tgctttcgat aaaatagaaa
     1501 agaaaaatgg ttaaaaacat tataataaag ataatgggta ttccacccaa ataacctcga
     1561 cccaaaagag taaaattctt tcctaacccc atgtggatac tattgccacc gctaataacc
     1621 aatgccaaac cccgagctgc atacatggta cctaaggtgg ctatgatcga aggaatcctc
     1681 aatttgatca ccatgagtcc gtttaaaaga ccgattgcgc ttcccaccaa agttgaaaga
     1741 ataattgaaa accatagggg aatcgattga gttacaaaga gggctgagag cattccggtg
     1801 agacccaata cacttcctat cgataaatca aagttaccag atatcatgag aggagtaact
     1861 gcacaaccag tgatcacgac aaaagccacc tgtcgggtta cgttgttgag gttggttacg
     1921 gttaaaaatg atttggatag gaaagaaagc acaataataa tcagcaacaa aacgccaaaa
     1981 ataaccattt tttgcaaaaa gccgctactc agcttaattt tcataattag tcctctttcc
     2041 acgggtgata atatatagaa tttcttctgg atccgcctct tggttcttta tttccttgac
     2101 aatttggcca tctcggagta aataaatgcg gtcacagagg ttaaatatct ccggatattc
     2161 tgaagaaaaa acgatgattg acttcccctt ctcggctaaa tttcttataa tacgatgaat
     2221 ttcctcctta gctccaatgt ctactccccg ggtgggttca tccatcataa gaatattcga
     2281 cccggcatgc aggtattttg aaaccacaac tttctgttga ttcccaccac tgagggttgc
     2341 tactttttgt tcaatatgtt ttactttgat attcatcgca tcaacataat ttcttgctac
     2401 ctcacgatct tcttggctac gtacgactcc aaaatgggaa agaaccttta aattggtaat
     2461 cgatatattg ggtcgaactg gaagcttcat aattaatcct tcggtaagcc gttcttccgg
     2521 taccatggat atcccgctca acatagcatc tcgaggaaga tcaaacagaa cactttttcc
     2581 ttcaatccgg atatctccgc tcaatactcg gtccaaccca aaaagagcca aggcaatttc
     2641 agttttccct gcccctctca aaccgtaaaa tcccaaaatc tcacccttaa atagattaaa
     2701 gttaacattt ttaagcttat gagttgtcag attgtatact tccaatacct tcttttgata
     2761 gtcaaccggt cgcgggacat atttatctac agtcacttta ccaatcatca ttcggatcaa
     2821 ttcatcccga tcaatatccg ccacattttt tgtttcaaca atttcaccat ctcgaaatac
     2881 tgtaaccacg tctccaatag taaaaatatc atccaaaatg tggctgatat aaattacggt
     2941 gatatttttc tccttcaagg aacgaataat ggcaaataaa cgtttggttt cattctctga
     3001 taccgctgca gttggttcat ccataattaa aaggctcgca tccacggcaa tcgctttgac
     3061 aatttcaata acttgttttt ccgcaaaact aagctgagct acttttttct tgagtggaat
     3121 gtcgggagca aactctttca tgagctgaaa aactttttcg gaaggatcac ttctttgaat
     3181 gacaccataa cgatttggtt ctctgcctaa aatgagattt tcttcaaccg tgagttggtt
     3241 taccacattc aattcttgaa acaaagtact aatccccaag tccatcgctt cccggatcga
     3301 gcgaggctta aattcttttc cattaaaaag gatttctcct gaacttctgg tttcagcaca
     3361 agtcaatatc ttaataaagg ttgactttcc tgcaccattt tccccaacaa tacaatgaac
     3421 ggtattgcga cgtatatcca aatcgattcc ttttaatgca gcaaccccgg gatagttttt
     3481 agctagtcct cgaacctcaa gtatgttttt ttccataaaa ttgactctcc taaatcaggc
     3541 aggaggaggt gtaactcctc ctgcctaaac ccctattcct attcttcaat ttctttagct
     3601 aaatcgactt ctattgccc
//
LOCUS       DAOSOL010000140         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26065_length_3587_cov_4.242072, whole genome shotgun sequence.
ACCESSION   DAOSOL010000140 DAOSOL010000000
VERSION     DAOSOL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26065_length_3587_cov_4.242072"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1645)
                     /locus_tag="PK171_04235"
     CDS             complement(56..1645)
                     /locus_tag="PK171_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOT05431.1"
                     /translation="MAEKIYLNIIWHMHQPYYYDSSQDIFTLPWVRTHATKDYLFMAK
                     LADRFPQVRMTFNFTPSLIKQINLYLQGKTDLVWNHFEKEAKKLSKKEKDFILENFFL
                     APSQTQTSHFPFYETLKEKAKHNIHNFSTQDWLDLQILYQLLWFDPITIKDNPDLSEL
                     IKRGKEYTEKDKAIIKQVTSKIIAEIIPMYKKLHDKGQIEISTSPLYHPIIPLLIDNW
                     VASESSPGTHLPRYRFQYIDDAQKQIQKAKDVAERIWKTEIRGIWPSEGSVSSAAVSC
                     FAQNGFSWTATGEEVLFHTLELPIERDQNGLLNQGEKLYQPWFFSNDKNNIAIFFRDR
                     HLSDLIGFAYQHLTFDDAVKDMISNLERIMNRLPNGYNPVLSIILDGENAWEYYNNNG
                     FDFLNNLYEALSQHSRITTTTPSEYLAHFDQKPALHTLAPGSWIYGSLNTWIGHEEKN
                     WAWDQLFLVRRLLAEKEKELDGERKQEIFNILYQAEGSDWFWWLGPDNPSVQKEDFRK
                     QFLSLLEKICDLIGEKYPGEG"
     gene            complement(1655..2152)
                     /locus_tag="PK171_04240"
     CDS             complement(1655..2152)
                     /locus_tag="PK171_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOT05432.1"
                     /translation="MTRITVLSDTHIPQRAKDIPQRLWEEIKQADLVLHAGDVVTPLV
                     LENIAQYTPIRAVRGNMDSVELQTVLPDQDLVEIEGVKIGLAHGRGAPNQVKFYVQSL
                     FEGQDLQVLVFGHSHRPELTKENGVWYLNPGSPTDKIFTPYLSYAQMEVDKGCITEIK
                     ILSLT"
     gene            complement(2297..3160)
                     /gene="folD"
                     /locus_tag="PK171_04245"
     CDS             complement(2297..3160)
                     /gene="folD"
                     /locus_tag="PK171_04245"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOT05433.1"
                     /translation="MKTTIIDGKKVSQEIREELKPRIQSLTQQGLKPGLAVILVGDDP
                     ASQVYVRNKEKACEKLGIYSLKHQLPSTTGQTELLHLIDELNSNPAIHGILVQLPLPK
                     HLDEKRILYHINPLKDVDGFHPYNLGRLMIGDPIFLPCTPWGVQELLSRYQIDISRKH
                     VVIVGRSNIVGKPLAMMLVQKAKAANATVTICHTGTENLAQHTRMADILIAAAGSPKM
                     ITGELIREGAVVIDVGINKVESQLVGDVDFESVTDRASFVTPVPGGVGPMTIAMLMAN
                     TVKSAERTLQR"
     gene            complement(3157..>3587)
                     /locus_tag="PK171_04250"
     CDS             complement(3157..>3587)
                     /locus_tag="PK171_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05434.1"
                     /translation="HPQVYRGEILSDYTSILIKALENPYINIIAHLGNPRYPVDYPLI
                     VKKAIDYNKVIEINNSSFHISRKGSLENCKMIAQEIKKQGGYIIVTSDAHYCDEVGDY
                     QLSLDLLESINFPKEYIINASPTMFQSFLNSFLKIRGRER"
BASE COUNT         1071 a          713 c          654 g         1149 t
ORIGIN      
        1 cgacgcccct ctccaccagc taatatcatc gctatcactt catttttaaa cactgctatc
       61 cctccccagg atatttctcg ccaattaaat cacaaatttt ttccaataga gataaaaatt
      121 gctttcgaaa atcctctttt tgtaccgagg gattgtctgg acctagccac caaaaccaat
      181 cacttccttc tgcttgatac aaaatattaa aaatttcttg ctttcgttcc ccatccaact
      241 ccttttcctt ttcagccaat aatcttctga ctaagaagag ctgatcccaa gcccaattct
      301 tttcttcatg tccaatccag gtatttaagg aaccataaat ccaggaaccg ggtgctaaag
      361 tatggagagc gggtttttgg tcaaaatgag ctaaatattc cgagggagtt gttgtggtta
      421 tcctggaatg ttgggataag gcttcatata aattattgag aaaatcaaac ccattattat
      481 tataatactc ccaggcattt tccccatcaa gaattataga taaaactgga ttataaccgt
      541 tagggagtcg gttcataatt ctttctaaat tggaaatcat atccttaact gcatcatcaa
      601 aagtaagatg ttgataggca aaaccaatca aatcggaaag atggcggtca cgaaaaaaaa
      661 tagctatatt gtttttatcg ttggagaaaa accaaggttg atagagtttt tcaccttgat
      721 ttaaaagacc attttgatct ctctcaatgg gtaactctaa ggtatgaaat aagacttctt
      781 caccagttgc cgtccaggaa aaaccatttt gagcaaaaca gctcactgct gccgagctaa
      841 ctgaaccctc cgaaggccaa attccacgaa tttcggtctt ccaaattctc tccgcaacat
      901 ctttggcttt ttgaatttgc ttctgagcat cgtctatata ttgaaaacga tacctgggaa
      961 ggtgggttcc gggtgatgat tcactagcaa cccaattatc gatcaataat ggaataatcg
     1021 gatggtataa aggcgatgtt gaaatttcaa tctgtccttt atcgtgtaac tttttataca
     1081 tcggtattat ttcagcaata atttttgaag taacttgttt aatgatggct ttatcctttt
     1141 cagtatactc ctttcctcgt tttatgagct cgctcaaatc gggattatct ttgatggtta
     1201 tcggatcaaa ccaaagaagc tggtagagaa tttggagatc gagccaatcc tgggttgaga
     1261 aattatgaat attgtgtttg gctttctctt tcaaggtttc atagaaaggg aaatggctgg
     1321 tttgggtttg tgaaggggct aagaagaaat tctctaaaat gaagtctttt tcttttttgc
     1381 tgagtttttt agcttctttt tcaaagtggt tccatactaa atcagtcttt ccctgtaaat
     1441 agagatttat ttgttttata agagacggag taaagttaaa agtcatacgt acttgcggaa
     1501 acctatcagc taatttcgcc ataaaaagat agtccttggt agcatgagtt ctgacccaag
     1561 gtagagtaaa aatatcttgg gaagaatcat agtaataggg ttgatgcata tgccagatga
     1621 tgtttaaata aattttttca gccattaatt cacctcaagt taatgataga atttttattt
     1681 cagtaatgca tcctttatca acttccatct gagcatagga aagataggga gtaaaaattt
     1741 tatcagtcgg acttcctggg ttcaaatacc atactccatt ctccttggtt aattccggtc
     1801 gatgggaatg accaaaaact aaaacctgta agtcttgtcc ttcaaaaagt gactgaacat
     1861 aaaacttaac ttgattgggg gcacctcggc catgagctaa cccaatttta accccttcaa
     1921 tttcaaccaa atcctgatcg ggtaaaacgg tttgtaattc aaccgaatcc atattccccc
     1981 gtactgcccg aatgggcgtg tattgagcta tgttttcaag aaccaagggc gtaaccacat
     2041 ccccggcatg taggactaaa tcagcttgtt ttatctcttc ccataatctt tgaggaatat
     2101 cttttgctct ttgaggaata tgagtatcag aaaggacggt tattcttgtc actcttttga
     2161 ctcactccca tataaagtat tttaggcatt aaaatattat tgaagagctt ttttgttgaa
     2221 cttgaatcca tcaacaccca aagtttaact ttataaaaaa catcattaaa atgcttattg
     2281 aagttttaaa ataaaattat ctttgtaagg ttctttctgc cgatttaacc gtattggcca
     2341 tcaacatggc aatcgtcatt ggccccactc cacctggaac tggtgtaaca aaagaagctc
     2401 gatccgtaac actctcaaaa tcaacatcgc cgactaattg ggattccact ttattgattc
     2461 ctacgtcaat gaccacggct ccttctcgga tcaattctcc cgtgatcatt ttaggggaac
     2521 cggcagccgc aatcaaaata tcagccattc gagtatgttg ggcaagattt tcagttccag
     2581 tatggcaaat ggttacggtg gcgttggctg ccttggcttt ttgaaccagc atcatagcta
     2641 acggtttccc aacaatatta cttcttccaa cgattactac atgttttctc gaaatatcaa
     2701 tctggtatcg agataacaac tcttgaactc cccagggcgt acagggtaaa aagattggat
     2761 caccaatcat aagacgacct aaattataag ggtgaaaacc atcaacatct ttgagtggat
     2821 taatgtgata aagaatcctt ttttcatcca gatgtttggg aagtggcaat tggacgagga
     2881 taccatggat ggctggattg ctatttaatt catcaatgag atgaaggagt tcagtttgac
     2941 cagtcgtgga agggagctgg tgtttcaagg aataaattcc cagtttttca caggcttttt
     3001 ctttgtttcg gacatatact tggctggcgg ggtcatctcc aaccaaaata accgccaaac
     3061 ccggtttcaa gccttgttgt gttaaacttt gaatccgtgg ttttaattct tcccgaattt
     3121 cttgtgaaac ctttttccca tcaattatag ttgttttcat cgctctctac ctcttatctt
     3181 aagaaatgaa ttcaaaaaag attgaaacat agttggagat gcattgataa tatactcctt
     3241 ggggaaattg atgctttcca acaaatctaa ggacaactga taatcaccga cttcatcaca
     3301 ataatgagca tcgctggtta cgataatata accaccttgc ttcttgatct cttgggcaat
     3361 cattttacag ttctctaagc tccccttgcg gctgatgtga aaggaactat tattaatttc
     3421 aatcactttg ttatagtcaa tcgccttctt cactatcaac ggataatcca ctggataacg
     3481 ggggtttccc agatgagcga taatattgat ataaggattt tccaaagctt taattaagat
     3541 tgatgtatag tcagaaagta tttctccccg atatacttga ggatgaa
//
LOCUS       DAOSOL010000141         3574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26204_length_3574_cov_3.205456, whole genome shotgun sequence.
ACCESSION   DAOSOL010000141 DAOSOL010000000
VERSION     DAOSOL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26204_length_3574_cov_3.205456"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..349)
                     /locus_tag="PK171_04255"
     CDS             complement(167..349)
                     /locus_tag="PK171_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT05435.1"
                     /translation="MALKIDEEACIGCELCVQVCPDVFEMNDDGKSIVKPGSDENKEC
                     VDEAIDSCPTSAIIKE"
     gene            complement(463..957)
                     /locus_tag="PK171_04260"
     CDS             complement(463..957)
                     /locus_tag="PK171_04260"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT05436.1"
                     /translation="MAHDVNWYWQKVEEVFHDDAHMIDHTKKVLEYAQQIISDQTDMS
                     EEEKRRTEVAVLLHDIGILVAEKKYGSRAGKFQHIEGPPLVREITKQAGEEPGFIDRV
                     AFIVGNHHNFSKADGTDFQILIEADMLVNLKNEKIIKNKLAEFIDNFFKTKKGREIAR
                     QIYL"
     gene            complement(960..1349)
                     /locus_tag="PK171_04265"
     CDS             complement(960..1349)
                     /locus_tag="PK171_04265"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOT05437.1"
                     /translation="MERDEIMGMVRDILDQLPDHIRENIINLEFIIEDRPNPEIKRQF
                     HGAMLLGLYQGIPLPKRGPGYTFVLPDRISLFYENLMKVVRDDEEWPGVLKDVVLHEI
                     GHYFGFNEMEIRKLMDEMKPEAERGMG"
     gene            complement(1365..2330)
                     /locus_tag="PK171_04270"
     CDS             complement(1365..2330)
                     /locus_tag="PK171_04270"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOT05438.1"
                     /translation="MKQFTKPTVVVSKCLGFDSCRYNGQMIPVDFIEKMKPYVHFVPV
                     CPEVEIGLGVPRDPIRLVSVNGDFRLMQPTTGHDYSDKMKHFALEFLQSLPEIDGFIL
                     KGRSPSCGVKDVKIYQSLDKGPSIGTTRGFFSEVVSNVSPFLPIEDEGRLSNLKIRDH
                     FYTSLFVLADFRQLRDQPSRQQLVQFHTELKLLLMAYNQSEMRMMGRIVAQVNKKQIK
                     EVYDDYRSHLLKALFRSPKTPSMINVFMHALGYFSTRLHTNEKAFFLDSLEKYRAGRS
                     TFATNLQLLRSWVIRFDEPYLQNQRFFEPYPEVLMELVDSSKGRE"
     gene            complement(2332..>3574)
                     /gene="phrB"
                     /locus_tag="PK171_04275"
     CDS             complement(2332..>3574)
                     /gene="phrB"
                     /locus_tag="PK171_04275"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOT05439.1"
                     /translation="DRKKVPFGVVFCLVNDFLGATRRQYDFMLKGLQQVHEELLRYKI
                     PFFYLKGNPSLVLPDFIKTNKISLLITDFDPLRMKRQWKAQLMEKSPVEIREVDAHNI
                     VPVWIASSKQEYGAYTIRPKISRLLSEFLDEYPLIQMNSSHQSWQGDSINWGDLFGNC
                     PGDSSLPPITQFIPGSRSALRQLQQFLKEKMDFYDNQRNDPNANAQSNLSPFLHFGQI
                     SAQRIALEVQKASIAKDKKAAFLEEVVIRRELSDNFCWYNLNYDQFAGFPSWAQQTLN
                     QHRKDQREYRYSLEEFEKAQTHDQLWNAAQKEMVYTGKMHGYMRMYWAKKILEWSETP
                     EIALQSTIYLNDRYELDGRDPNGYAGIAWSIGGVHDRAWGERPVFGKIRYMSYRGSRS
                     KFDVDQYISQYKDWEESVRKD"
BASE COUNT         1068 a          762 c          613 g         1131 t
ORIGIN      
        1 gaaagggcgg tgtccttacc aggctagacg atgagggcgc acatatatat tttactaagt
       61 cgaacaagca gtgattattc tatagaaatt atcctttaat atcaagaccc tgaatcaaaa
      121 attctcaaaa aattttaaaa aagcccggtt ttgccgggct gaataatcat tccttaatga
      181 ttgcacttgt gggacaagaa tcgattgctt cgtcaacaca ttctttgttt tcatcacttc
      241 caggttttac aatgcttttc ccatcatcat tcatttcaaa aacatcggga catacctgaa
      301 cacataattc acaaccaata caagcttctt catcaatttt tagagccatg agctattcct
      361 cctgtttatg gtgttgaaaa atcacgcaac attataccat atctgttttc gaaccaaagt
      421 taaattgtag gaaaaaaata aaaaaattga gactcatttt cattacaaat aaatttgccg
      481 tgctatttcc cttccttttt tcgttttaaa aaaattatca ataaattccg ctagtttatt
      541 ttttataatc ttttcatttt tcaaattaac caacatatca gcttcaatta gaatttggaa
      601 atctgtccca tctgctttgg aaaaattatg gtgatttccg actataaatg ctacccgatc
      661 tataaagcct ggttcctccc cagcttgttt ggttatttct cgaaccagag gcggtccttc
      721 tatatgttga aacttgcccg ctcgagaacc atattttttc tcggctacca atattcctat
      781 atcgtgaaga agaacagcaa cctcggttcg tcttttttcc tcttcgctca tatcggtttg
      841 gtcggagata atttgttgag catattccaa gacttttttg gtatgatcaa tcatatgagc
      901 atcatcatga aaaacttcct ccaccttctg ccaataccaa tttacatcat gagccatctt
      961 caacccatcc ccctttcggc ttctggtttc atttcatcca ttaatttccg gatttccatt
     1021 tcattaaagc caaaataatg tccaatttca tgtaaaacga cgtcttttaa aactcctggc
     1081 cattcttcat catctcggac cactttcatt aagttctcat agaataatga tatccgatcg
     1141 ggaagaacaa aagtataccc cgggcctctt ttaggaagag gaattccctg ataaagtcct
     1201 aataacatcg ctccgtgaaa ttgccgtttt atttccggat tcggacgatc ctcaataata
     1261 aactctaaat ttataatatt ttctcgaata tggtcaggga gttgatcaag aatatctcga
     1321 accattccca ttatttcatc tctttccata ataactcccc ctctctattc tcgaccctta
     1381 ctagaatcca ccaattccat gagtacttcg ggataaggtt caaaaaaacg ttgattttgt
     1441 aaataaggct cgtcaaagcg aatgacccaa gaacgaagga gttgaagatt ggtcgcaaag
     1501 gtacttcgac ctgctcgata tttctccaaa gaatcgagaa aaaaagcctt ctcattggta
     1561 tgaagtcgag tggaaaaata acccagggca tgcataaata cattaatcat cgagggagtc
     1621 ttgggtgaac gaaaaagtgc ttttaaaagg tgagatcgat aatcgtcgta aacttccttt
     1681 atttgctttt tattcacttg agcaacaatt ctccccatca tcctcatttc gctttgatta
     1741 taagccatta ggagcaactt aagttcggta tggaactgaa ccagttgttg tcgtgaaggt
     1801 tgatcacgca gttggcgaaa atctgctaaa acaaataagg atgtataaaa atgatcgcga
     1861 atttttaaat tggataaccg cccctcatcc tcaattggta aaaaggggga aacattagaa
     1921 actacctcgc taaagaatcc tcgagttgtc cctatggatg gacccttatc caaactttga
     1981 tagatcttga catctttaac accacatgag ggagatcgac ccttgagaat aaaaccgtct
     2041 atttcaggta aactttgcaa aaactccaag gcaaagtgtt tcattttatc ggaataatca
     2101 tggccggttg tcggttgcat cagtctaaaa tctccattta cggatactaa acgtattgga
     2161 tcgcgcggaa ctcctaaacc aatttccacc tcaggacaaa ccggaacaaa atgaacatag
     2221 ggtttcattt tttctataaa atcaactggt atcatttgcc cattgtatcg acaagaatca
     2281 aagcccaaac atttgctcac taccacggtt ggcttggtaa attgtttcat ctcaatcttt
     2341 cctcactgac tcttcccaat ctttatattg agagatgtat tgatcaacat caaacttaga
     2401 gcggctcccc cgatagctca tgtatcggat ttttccaaac actggtcttt caccccaggc
     2461 acgatcatgc acacctccaa tgctccaagc aataccagcg tatccatttg gatctcttcc
     2521 atctaactca tatcggtcat ttaaatagat ggtactctgc aaggctattt caggagtttc
     2581 cgaccattct aagattttct ttgcccagta catcctcata tatccatgca tcttgcccgt
     2641 ataaaccatt tctttttgtg cggcattcca aagttgatca tgagtttggg ctttttcaaa
     2701 ttcttctaat gagtatcgat actccctctg gtctttgcgg tgttgattaa gagtttgctg
     2761 tgcccaagat gggaatccag caaattgatc atagttaaga ttgtaccagc aaaaattatc
     2821 cgaaagttct cgcctaataa caacttcttc taaaaaggct gcctttttat cttttgcaat
     2881 cgatgctttt tgaacttcca gggcaattct ttgtgcggaa atttgaccaa aatggagaaa
     2941 gggggaaaga ttcgactgag cattagcgtt gggatcattg cgttggttgt cataaaaatc
     3001 cattttttct ttaagaaatt gttggagctg tcgtaaagcg cttcttgaac cgggaataaa
     3061 ctgggttatc ggtggaaggg atgagtctcc tggacagtta ccaaacaaat cgccccaatt
     3121 gattgaatcc ccttgccacg attggtgtga agaattcatt tgtattaatg gatattcatc
     3181 aagaaattca gataataatc gagatatttt cggtcgaatc gtataggctc cgtattcttg
     3241 tttagaagaa gcgatccaca ccggcactat attgtgagca tcaacttctc gtatttcaac
     3301 tggagatttt tccatcaatt gagctttcca ttgccttttc attcggagag gatcaaaatc
     3361 ggttatcaat aaagaaattt tattagtttt gataaagtcg ggcaatacca agcttggatt
     3421 tcctttgagg taaaaaaaag gaattttata ccttaagagt tcttcgtgaa cctgctgtaa
     3481 acctttcagc ataaaatcat attgacgtcg ggtcgcaccg agaaaatcat taaccaggca
     3541 gaaaactact ccgaatggga ccttttttcg atca
//
LOCUS       DAOSOL010000142         3570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26241_length_3570_cov_3.595733, whole genome shotgun sequence.
ACCESSION   DAOSOL010000142 DAOSOL010000000
VERSION     DAOSOL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26241_length_3570_cov_3.595733"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="PK171_04280"
     CDS             complement(<1..831)
                     /locus_tag="PK171_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT05440.1"
                     /translation="MSKLRIIAILTILILAFSSTITISFSQPLSATDATGFTLSLSQP
                     PNRIISLAPGNTEILFALGLNDKIVGVTTYCDEPIEALKKEKIGNVTEIDLEKILTLQ
                     PDLILASSLTPEETVARLHELSLPVFTLKSDTIEEVIEDITKVAVLSGVEISGSNLIK
                     TMKDQLKKINDLSQSIPDYQKPLVFHIIWHDPIWTAGNSTFINEFITLAGGKNLAEDI
                     QGYSTIDFEEILKRNPSIITVIENHGKNEENQLYQFVTTDNRLQTVDAIKNNQVYLID
                     S"
     regulatory      complement(957..1131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1762..2961)
                     /locus_tag="PK171_04285"
     CDS             complement(1762..2961)
                     /locus_tag="PK171_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOT05441.1"
                     /translation="MLALIKILIIFAFILVLIKKKLNIGLSLILASFLFWILFQIPFS
                     KFPSVFPLGFFAYENTNLLLSVFSIMLLGNLLSNNGELVKLVRGIEERIRDSRLVSPA
                     LSALIGLLPMPGGALFSAPLIEASYSSSSLSSQLLTFINYWYRHVWEYFLPLYPVILM
                     ASNLTGLSLAWIIIHHLPFTLLALIGGWIVIKNQKPLSKANIKTESMPLKTLLRLFVP
                     ILIPVFVVLLNQPVWLGAAMGIVFCVFFHRMNLTTLSRLMFHRFSWAMMLDVVGVILF
                     KTVIIESGSIPVLVDSFLSSHFPILIIGMVIPFLIALISGYSSAFIGIAFPIILPLFT
                     EYSIQQIFPLIFISGYAGITFSPAHLCLILTCRHFKSDLISTYRQLFLPFLLVFSWGV
                     IWFFLAR"
     gene            complement(2967..>3570)
                     /locus_tag="PK171_04290"
     CDS             complement(2967..>3570)
                     /locus_tag="PK171_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT05442.1"
                     /translation="LIELLDILSIKKVIIIGHSLGGNLALRMALLHPERVQALFLISP
                     AVFSVHGVPLSSLIFANPLLRTGIISIANKTLRSEIKLTKTLQDAVFDKNIIDQDLIE
                     RIVKPILRNPKSGLALYYYLRDSSRNHVFPDLKSLTLPIFIMSGREDSWVKISEIRRL
                     NASLNNVTFLEIYQCGHMPQEEKPDKVVKQLIKFTDLHSQ"
BASE COUNT         1196 a          628 c          736 g         1010 t
ORIGIN      
        1 agaatcaatt aaatagactt gattattttt tatcgcatct actgtctgaa gccggttatc
       61 ggttgtaaca aattgataga gttgattttc ctcatttttt ccatgatttt caataacagt
      121 aattatagaa ggatttcttt ttaaaatctc ttcaaagtct attgttgaat aaccctgaat
      181 atcctcagcc aaatttttcc caccagctaa agttataaat tcattgataa aggtactgtt
      241 gccggctgtc cagataggat cgtgccaaat aatgtggaaa accagtggtt tttgataatc
      301 tggaattgat tgagagagat catttatttt tttaagttga tccttcatgg tttttatcag
      361 gtttgaacca gaaatttcca ccccggataa gaccgcaact ttggttatat cttcaatgac
      421 ctcctcaatg gtatctgatt taagagtaaa gaccggaaga ctgagttcat gaaggcgggc
      481 aaccgtttct tcgggggtta atgaactggc taagatcaag tcaggctgaa gggtaaggat
      541 tttttccaaa tctatttcag taacattccc aattttttcc ttttttaaag cttctatagg
      601 ctcatcacaa taagtagtca ctccaacaat tttatcattt aaaccaagtg caaaaaggat
      661 ttcagtattt ccaggggcga gagaaataat gcgatttgga ggttgggaga gactgagagt
      721 aaatccagtt gcatcggtgg cacttaatgg ctgagaaaat gatattgtta ttgtggaaga
      781 aaaggctaaa attaatatgg ttaaaatagc aataatcctg agtttactca ttttattctc
      841 cttatttgtc attagcctga ttagttctac gtggaaagaa gagggagatg agggagggtt
      901 tcccaagatt ctggtggcgg tccagaagct ttttcccacc tcctctttcc gcgaagagtg
      961 gcaaggatgt tttggacggg tctcctgact tctgaataat catctggctg tgccttccca
     1021 gttataataa ccagtggcat atacaaccag cattttcagt tacagtggcg ggaccgcacc
     1081 ggactcacac cggacttccc cgagtaaaca accaaaacaa ctttgcggtg agtatatcat
     1141 attcgaatca tatcaacaac aatttcagac cttatcttgg aaatgaatat tagaagattt
     1201 acaattgatc tttacgccat tatatgaaac cagatgagga tgaaaatacc tatttaaatc
     1261 cgtcattgcg aggagtttaa ccagcttttg gttagacgac gtgagaatct catccacccc
     1321 cactccgtca ttctaaggag tgtcttgtgc gacgtgagaa tctcatcctt ttatatatgt
     1381 ttctgtatca ttttctcagg taaccaagaa aaaccaaaag gcaccctccc cgccgtaaaa
     1441 cggcacccct ccaaggaggg gaattgttga atttattccc ccattgaggg gggattcagg
     1501 ggggtgtgct tttcttattt tgtttcatca tttatcattt atttcggtat tttaaggata
     1561 gaccctcatg ctgtccagca acaccagatg aggatgaaaa actaaagatt cgacatgcca
     1621 tggcaggtcg ctacaataat cgggcgcaat taattgtgca cctacgtaaa aaattctttg
     1681 gtaggggttt gatctatcaa gtcggtgatt ttctaattaa tcaaatacca tcgactcctt
     1741 attaatgttc ttgttgagct attatcgagc gaggaaaaac caaataaccc cccaagaaaa
     1801 taccaataga aagggcaaaa agagttgtcg gtaggtagaa atgaggtcgc ttttaaaatg
     1861 gcggcaggta agaattaaac aaagatgagc tggtgaaaaa gtgattccag cataaccaga
     1921 aataaatatt aaaggaaaaa tctgttggat ggaatattca gtaaacagcg gaaggataat
     1981 tggaaaggca attccgataa aagctgatga ataaccactg atgagggcaa ttagaaaggg
     2041 aatgaccatc ccgataatca atataggaaa atgactagaa agaaaggaat ctactaagac
     2101 cgggatgctc ccactttcaa taatgacagt tttaaaaagt atgaccccaa ccacatccag
     2161 catcatagcc caggaaaacc gatgaaacat caatcgtgat aaggtggtaa ggttcatacg
     2221 gtgaaaaaag acgcaaaaga caattcccat tgcagctccc aaccaaaccg gttggttaag
     2281 gagtacgaca aaaactggaa taaggatagg tacaaataac cgaagcaaag ttttcaaggg
     2341 catagattca gtttttatat tggcttttga taggggtttt tggtttttta tgactatcca
     2401 accaccgatt aaagctaata gggtaaaggg tagatgatga ataataatcc aagcaaggga
     2461 taaacctgtt aggttagatg ccattaaaat aactggataa agcgggagga aatactccca
     2521 tacatgtcga taccaataat taataaaggt aagcaattgg cttgataatg aactggatga
     2581 ataagaagct tcgattaaag gagcggaaaa aagtgcacca ccgggcatgg gaaggagccc
     2641 gattagagct gataaagctg gggataccaa tcgggaatca cgaattcgtt cttcaatacc
     2701 tcgcactaac ttaacaagtt ccccattatt ggacagtaaa tttcctaaca acataataga
     2761 aaaaacactg agtaataaat tggtattttc ataagcaaaa aaccctaaag gaaaaaccga
     2821 cgggaatttc gaaaagggaa tttgaaataa aatccaaaaa agaaaagaag ccaaaattag
     2881 acttaaaccg atattcaatt tctttttgat gagaactagg ataaaagcaa aaatgatcag
     2941 tattttgatg agagcgagca tggaatctat tgggaatgaa gatcggtaaa tttaatcaat
     3001 tgtttgacaa ctttatctgg tttttcttcc tggggcatat gaccacattg gtaaatttct
     3061 aaaaaagtga cgttattgag ggaagcgttt aatcgtctta tttcggagat ttttacccaa
     3121 gaatcttccc taccgctcat aataaaaatc ggcaaagtaa gactttttaa atcaggaaaa
     3181 acatggtttc gagaagaatc tcgcaaatag tagtagagag ctaagccact ttttggattt
     3241 cgaagaattg gtttaactat tcgttcgatc aagtcttggt caattatatt cttatcaaaa
     3301 actgcatcct ggagggtctt tgtcagttta atttcggacc gcagtgtctt attagcaatc
     3361 gaaataatac cagttctcaa tagaggattg gcaaaaatta aacttgacaa ggggactcca
     3421 tgaaccgaaa ataccgcggg actgatgaga aatagagctt gtactctttc gggatgaaga
     3481 agagccattc gaagagcaag gtttccccct aaagaatggc cgataatgat aacttttttg
     3541 attgaaagaa tatcgaggag ttcgataagc
//
LOCUS       DAOSOL010000143         3546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26472_length_3546_cov_3.835864, whole genome shotgun sequence.
ACCESSION   DAOSOL010000143 DAOSOL010000000
VERSION     DAOSOL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26472_length_3546_cov_3.835864"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK171_04295"
     CDS             complement(<1..249)
                     /locus_tag="PK171_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05443.1"
                     /translation="MDIKTKRKLNQLFLWIAVAVILIAVLAPFLWLVISSISHKVDLT
                     SVPLRFFPKKPTFENYLKIFYGGRGVTDAASQFGQCTIN"
     gene            complement(252..1181)
                     /locus_tag="PK171_04300"
     CDS             complement(252..1181)
                     /locus_tag="PK171_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05444.1"
                     /translation="MDTLTNNYSLKKRLSLFFLEKKNGVAYLLLVPSLVLIFGILIYP
                     LLYSLFISFHDYLLTKPGIYNFIGFANYLEALQSSFFLKSVVRTLYFAFATVGLELVL
                     GLGVALVLQQKFRGRGLVRGLIILPWALPTSVNGIMWKWIYNANYGVLNALLKRLGVI
                     ERYQVWLADPTRALHLVMLANIWKETPVGVLMFLAVLEQIPRELYEAALVDGANTLKK
                     FWYITLPMLKPMIVTLAIIKIIWAIREFDIFYIITRGGPGEGTSVLSYYAYLNSFKFS
                     RMGFGSAIAYLVIVLVIFIGIPYFRYMRKQEVD"
     gene            complement(1238..2620)
                     /locus_tag="PK171_04305"
     CDS             complement(1238..2620)
                     /locus_tag="PK171_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05445.1"
                     /translation="MITPRMGFVIYGVHKDGLKDPMGNLFIDEKVIAQSKKALNDRGV
                     ELVEYPIVVATKEEARLALKTMKDDEKIDGVILFSGTWIWASNMIAAIRDYGMTGKGI
                     CIWTHPGSQGWRPVGGLVLHGALLEVGIPHKFVYGLADEAHEIDRIVHFSQACHLKNR
                     LNQATIGAFGGRGMGQTVGVADPSQWMRVFGLDIDSRDTTELIRTAEEVTPDQLKKLE
                     PRIIKLFGNLPESSVVNERSLRLYLAIKTLVEKEKWEFYTIQSFPGLGDDYSATCFAQ
                     SMMLEDRVGTSTLGDFNTALTVYLMTRLSAEPVYYGDLQHIDKKHREIKIIGDGAIPP
                     SLAGKVGPAGFSVHGIPTEGQAGGLSIRAVAKVGKGILARLGRVNGEFQMVIVPVSIF
                     EPSQETIMSRLDECGIPFWPHGFVTVEGDIDCLLENWMNEYACLGYGEELYPVMVEFC
                     KLTGITVVLP"
     gene            complement(2661..3455)
                     /gene="budA"
                     /locus_tag="PK171_04310"
     CDS             complement(2661..3455)
                     /gene="budA"
                     /locus_tag="PK171_04310"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOT05446.1"
                     /translation="MDQYWIKKPISSIFTGFLFLFFFNPLLWAANSSPFFQFSTINAL
                     LEGVYDGELTIQELLQKGEIGLGTFDALDGEMVIINGRCFRVNSQGIVEEVTLDETTP
                     FAAIAQASFSNSHYLFLIDSLEELESELDQIIENPNLIHIFHIEADFQTLKARSVHRQ
                     TKPYPPLTQVTPNQAVFEFTEMSGDLVGFWCPQFFEGLNVSGYHFHFIDKDLKHGGHL
                     LDCSFLLGTVHISPCYDFFVQLPHNQEFARANLAGKRIDEIQQVEK"
BASE COUNT         1135 a          785 c          630 g          996 t
ORIGIN      
        1 atttattgtg cactgaccaa actgagaagc agcatcggtt actccccgac caccataaaa
       61 tatcttcaaa taattctcaa aagttggctt tttggggaaa aaacgtagtg gaacactggt
      121 taaatctacc ttatgggata tactggatat aactaaccaa agaaaagggg ctaacactgc
      181 aataaggatt actgcgacag caatccaaag gaataattgg ttcaatttcc ttttcgtttt
      241 aatatccatc tttaatcaac ctcctgcttt cgcatatagc gaaaataggg gataccaata
      301 aaaataacta gaacgataac taaataagca atggcacttc caaatcccat ccgtgaaaat
      361 ttgaaactat taagataagc gtaatagctt aagacactag tgccctctcc aggaccaccc
      421 cgggtaatga tatagaaaat atcaaattcc cgaattgccc agatgatttt aatgatggct
      481 aaagtaacaa tcatcggctt tagcatggga agggtgatat accaaaattt ctttagggta
      541 ttcgctccat cgactaaggc tgcttcgtag agttcgcgcg gaatttgttc taaaactgcc
      601 aaaaacatca atactccaac cggtgtttcc ttccagatat tggctaacat gaccaagtga
      661 agtgcccggg ttggatcggc caaccagact tgatagcgtt caatcacccc taaacgcttt
      721 aaaagggcat ttagaacccc ataattggca ttatatatcc atttccacat aatcccattc
      781 actgaagttg gcaatgccca aggaagaatg atcaaacctc ttaccaaacc tctccctcga
      841 aatttctgtt gaagcactaa agctacccct aaacccaaaa ccagttcaag tcctacagta
      901 gcaaaggcaa aataaagggt acgaacgacg ctttttaaaa agaaactact ctgtaaagct
      961 tcaagatagt tggcaaatcc aataaaattg tagattccgg gcttagtgag aagatagtcg
     1021 tgaaaactaa taaaaagaga atagaggagg ggatagatta gaatcccgaa tataagcact
     1081 aaacttggta ccaacaataa atacgcaaca ccatttttct tttctaaaaa gaaaaggctc
     1141 agtctttttt tcagagaata gttattggtt agtgtatcca tctcgacttc aattatccct
     1201 ccctcatctt atagtcaata aagctattta aaacctgcta cggtaaaacc actgttattc
     1261 cggttaattt acaaaattct accatcaccg gataaagctc ttctccatat cccaaacaag
     1321 catactcatt catccaattt tccagtaaac aatcaatatc accctccact gtaacaaagc
     1381 catgaggcca gaaaggtatc ccacattcgt ccaatctact cataattgtt tcctgagatg
     1441 gctcaaagat cgatactgga actatgacca tttgaaattc tccgtttact cttcctaaac
     1501 gagccagaat tccctttcca actttcgcaa cggctcgtat cgataatcca ccggcttgcc
     1561 cttcagtcgg aattccgtga actgaaaatc ctgcagggcc aactttccca gctaaagaag
     1621 gtggtatggc accatctcca atgatcttaa tctcccgatg ttttttatcg atatgttgaa
     1681 gatcaccata atacaccggt tcagcactta aacgggtcat aagataaacc gttaaagcgg
     1741 tgttaaaatc tcccagggtt gaggttccta ctcgatcctc aagcatcatt gactgggcaa
     1801 aacaggtagc ggaataatca tctcctaatc cagggaagga ttggatggtg taaaattccc
     1861 atttttcttt ttcaaccagg gttttgattg ctaaataaag gcgaagagac cgttcattca
     1921 ccaccgatga ttcgggcaga ttgccaaaga gtttgattat acgtggttca agctttttaa
     1981 gttgatcggg ggtcacttct tcagcagtac gaatgagctc ggtagtgtct cgagaatcaa
     2041 tatctaaacc aaaaactctc atccattggg aagggtcagc aacaccaacc gtttgtccca
     2101 ttcctcgtcc accaaaagct ccaatagtag cttggtttaa gcgattcttc aaatggcatg
     2161 cctgactgaa atgaacaatt ctatctattt cgtgggcttc atcggctaat ccataaacaa
     2221 atttatgagg aatccctact tccaggaggg ctccatgcaa aaccaagccg cccactggtc
     2281 gccatccctg cgatcctgga tgggtccaga tgcaaatgcc ttttccagtc attccataat
     2341 ctctgatggc agcaatcata ttcgatgccc aaatccaagt accggaaaat aaaataactc
     2401 catcaatctt ttcatcatct ttcatggttt tgagtgctaa tcgggcttcc tctttggttg
     2461 ccaccacgat agggtattca accagctcaa cgcctcgatc gtttaatgct ttctttgatt
     2521 gagcgatcac tttttcatca atgaaaagat tccccattgg atcttttaat ccgtctttgt
     2581 gaactccgta aataacaaaa cccattcttg gtgtaatcat aaaaatactc ctattctttt
     2641 aacccgtatt cgggaatttt ttatttttca acctgttgaa tttcatcaat cctcttgcca
     2701 gcaaggttgg ctctggcaaa ttcttggttg tggggaagct gtacgaaaaa atcatagcag
     2761 ggtgatatat gaacagtgcc taacaaaaaa gaacaatcca ataggtgacc accatgtttc
     2821 aagtctttat cgataaaatg aaaatggtag ccggatacat tgagaccttc aaaaaattgt
     2881 gggcaccaaa aacccactaa atcaccgctc atttctgtga attcaaaaac tgcttggtta
     2941 ggagtaacct gagtaagtgg tggatagggc ttggtttggc gatgcacact acgagccttg
     3001 agagtttgga agtcggcttc aatgtgaaaa atatgaatca agttgggatt ctcaattatt
     3061 tgatccaatt ccgattccag ctcttcaagg gaatcaatta aaaacaaata atgagaattg
     3121 gagaaagaag cctgagctat agccgcaaaa ggggttgttt catccaatgt cacttcttcg
     3181 acaatccctt gactattcac tcgaaaacat cgaccattga ttatcaccat ttcaccatcg
     3241 agagcatcaa aagttcctaa gcctatctct cctttttgta agagttcttg aatagttaat
     3301 tctccatcat atacaccctc aagaagagca ttgatggttg aaaactgaaa gaagggagaa
     3361 gaattggccg cccaaagcag aggattgaaa aaaaacaaaa ataaaaatcc ggtgaagatc
     3421 gaggaaattg gttttttaat ccaatattga tccaaatttt tgaccttcct tcaattaatc
     3481 gtatccatcc gatacaatat tttttaaaat attataacct atattttcat cgctcttata
     3541 aggccc
//
LOCUS       DAOSOL010000144         3538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26549_length_3538_cov_3.180304, whole genome shotgun sequence.
ACCESSION   DAOSOL010000144 DAOSOL010000000
VERSION     DAOSOL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26549_length_3538_cov_3.180304"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..980)
                     /locus_tag="PK171_04315"
     CDS             complement(117..980)
                     /locus_tag="PK171_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT05447.1"
                     /translation="MVLKNGVKPSEKIKIYTLVDNTAGYGFQGEWGLSFYIEVHGHKI
                     LFDTGMTSLASENARLAKIDLSSIDTLVLSHGHNDHTGGLAHFLKYIGDVPIIAHPDI
                     WTKKYVQENQNSLDYIGIPACREELESKGAQFHLTTKTFKICQSIITTGEIELNNSYE
                     KIDSDLLVKTAGEFSPDSMMDDVSLIIQTERGLVVVTGCAHRGIVNTLQHAQRITGDQ
                     RILYVIGGFHLYQASHERLDWTIQTLFTMGVQKVSPCHCTGFQAMAQIYQNMPKYYQP
                     IYAGSVIEIET"
     gene            complement(987..2459)
                     /locus_tag="PK171_04320"
     CDS             complement(987..2459)
                     /locus_tag="PK171_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05448.1"
                     /translation="MNGINPYNMALANPENDTKVTFHTLRINDEEMDYSATVGSIPII
                     DQKSQTEQARIYYITYNRQGIENFEKRAITFAFNGGPGAASMMLHLGVLGPRVVEKSK
                     DGTRLMPPPFRLVDNPNTLLDITDLVFIDPIGTGYSRMTGGADPTQYWGVSEDVRCIA
                     QFIQSYLNHNGRSTSPVIIAGESYGGVRGSGLAKVLQDIGVYPSGLIFISPVFDLGTI
                     QWSSLDDRALALTIPTYAAAAWYHKKINSDYQGDLDSVLQEVRAWVENHYLKALWRGN
                     ALSEEERDKIVEKLSQYTGIAEDFIREKNLRVYEDDFASELLNDKGLALGIYDARVTI
                     PGTYVDEDDPSYFLIGGPFKSCLANYLKNDLQFESNLSYLSGSAEVYEKWNWESGRPA
                     PKYEGTVSLGYPNQNEELSKALRRSDFLKVFIASGVYDLECPYDSVLYSINHLDLPAE
                     RRNNITLQIYPGGHMLYTNPEALTQLKKDLEEFYQDILGE"
     gene            complement(2724..>3538)
                     /locus_tag="PK171_04325"
     CDS             complement(2724..>3538)
                     /locus_tag="PK171_04325"
                     /inference="COORDINATES: protein motif:HMM:NF016784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05449.1"
                     /translation="LMQSSPGFIDSFADFFITGAGIVGVETTIGGFNVYEENEAPEFY
                     RARKAMQYGNSLDDFVAIMKKQNNGGYANSWLLGDIKSGEIMRFELGLKFFNIERKKD
                     GYFIGINAAFDDRIRNLECVGSNFVDIRKPSGSRRVRLTQLMNEYKGKINVEVAQRIL
                     ADHYDVYLQKEKPGYRTIDSHYYLDAFEYVSTSGSHPVPFEPFGTLDGKVTDSQLAQQ
                     FAFWGRWGNSSGLEFNAQKFLSEHFQWEHLSGYLKDRPSQPWTLFQAGKIPK"
BASE COUNT         1074 a          771 c          594 g         1099 t
ORIGIN      
        1 aaaaggatga gattctcacg tcgcacaata cgctcctcag aatgacggag taggtggatg
       61 atatgccacg tcgctgcaca ctgaaaatca gcgatcaata ctcactggct tccaagctaa
      121 gtttcaattt caataactga tccagcgtaa atcggttgat aatatttcgg catattttga
      181 taaatctgag ccatagcttg gaatccagta caatgacaag gactcacttt ttggacaccc
      241 atagtaaaca gggtttgaat cgtccaatcc aatcgctcat gagaggcttg gtaaagatgg
      301 aaccctccaa taacgtataa aattcgctga tccccggtaa ttctttgagc atgctggagt
      361 gtattcacaa ttccccgatg ggcacaacca gtgacaacca ccaaacctct ttcggtttgg
      421 ataattaaag acacatcatc catcattgaa tcaggagaaa attctccagc agttttcacc
      481 aacaaatcac tatcaatttt ttcataagaa ttgtttaact ctatttcgcc ggtcgttata
      541 atagattgac aaattttaaa agtttttgtg gttagatgaa attgtgctcc cttgctttcc
      601 aattcttctc gacaagctgg aattccaata taatctaaag aattttggtt ttcttggaca
      661 tattttttgg tccaaatatc cgggtgggca ataattggaa catccccaat atattttaag
      721 aaatgagcca accctcctgt atggtcatta tgcccatgac ttagaaccaa agtatcgatc
      781 gatgaaaggt cgatttttgc tagtcgagca ttttcggaag ccagcgaggt cattccggta
      841 tcaaatagaa ttttatgtcc atgaacttct atgtaaaaac taagccccca ttctccctga
      901 aaaccatacc cggctgtgtt atcaaccaag gtatagattt taattttctc agagggcttc
      961 accccatttt tcagcaccat taaaaatcac tctccaagaa tatcttgata aaattcttct
     1021 aaatcctttt ttaattgggt aagggcttca ggattggtat agagcatatg acctcctgga
     1081 taaatttgaa gagtaatatt atttcttcgt tccgctggta agtccagatg gtttatactg
     1141 taaagaacgg aatcataagg acattccaaa tcatatactc cactggcaat aaacactttg
     1201 agaaaatcag aacgacgtaa ggcttttgac aattcttcat tttgattcgg atatcctagg
     1261 cttaccgttc cctcgtactt aggagccgga cgacctgatt cccaattcca tttttcatat
     1321 acttctgctg aaccagaaag ataggaaagg ttgctttcga attgaagatc gtttttgaga
     1381 taattggcca agcaagactt aaaaggacct cctatcagaa agtaactggg atcgtcttca
     1441 tcgacataag tcccgggaat tgtaaccctt gcgtcgtaaa ttcctaacgc taaaccctta
     1501 tcattgagta attcagaagc aaaatcatct tcataaactc ttaaattctt ttctcgaatg
     1561 aaatcctcag caatgccagt atattgactt agcttctcaa ctattttatc tctttcctct
     1621 tcgctcaagg catttcccct ccaaagtgct tttaaataat gattttcaac ccaagctctg
     1681 acttcttgta acaccgaatc taaatctccc tggtaatctg aattaatttt tttatggtac
     1741 caggcagcgg ctgcataagt aggaatggtg agcgctaaag cacgatcgtc taatgaactc
     1801 cattgaattg ttcctaaatc aaaaaccggt gatataaata tcaatcctga gggataaact
     1861 ccaatatctt gaagaacttt ggctaatcct gagcctcgta ctccaccata actttctcca
     1921 gcgataatta ccggtgacgt actccggcca ttatgattca agtaggattg gataaattgt
     1981 gcaatacacc gaacatcttc actgactccc caatattgag ttggatcagc tcctccggtc
     2041 attcggctat acccagtgcc aattggatca ataaaaacca ggtcggtgat atctaataga
     2101 gtattaggat tatcaaccaa tcgaaaaggt ggtggcatta atctggttcc atctttactt
     2161 ttttccacta cccgtggccc caaaactccc aaatgaagca tcattgatgc cgctcccggt
     2221 cctccattaa atgcaaaagt aatagcgcgt ttttcaaaat tttcaattcc ttgtcggtta
     2281 taagtaatat aataaattct ggcttgttcg gtctggcttt tttgatcaat aattgggata
     2341 gatccaactg tagctgaata gtccatttct tcatcattaa tcctcagggt atgaaaggtg
     2401 actttggtat cgttttccgg gttagccaat gccatattgt atggatttat tccattcaat
     2461 ccgatagata cactgagaaa tccgaaaaaa agaaggatca ccccaaatct ttttgttcaa
     2521 aaagattatg tcataaatca tcctaaggtt tcctcccaat tttctcaatt aagcggaatt
     2581 ttataattat ttctttgcta aacaagagat atcaggctta atcaattaaa tcttcgcaaa
     2641 atcagttctt tatcaaacca aattttccct cttgatttta tcaatattcc acttcccata
     2701 aagattgcca agaaacctta acattatttt ggtatttttc ctgcttgaaa aagggtccag
     2761 ggctgagaag gtcgatcttt taaatatcca ctaagatgtt cccattggaa atgttcggaa
     2821 aggaattttt gagcgttaaa ctccaaacct gaagagttgc cccaacgtcc ccaaaaagca
     2881 aactgctgtg ccaattggga atcggtcacc ttaccatcca aggtaccaaa aggttcaaaa
     2941 ggtaccggat gtgatccgct ggtgcttaca tattcaaaag catcgagata ataatgacta
     3001 tcaatagttc gatatcccgg cttctctttt tgaagatata catcatagtg atcagctaag
     3061 atcctttgag ctacttcaac atttattttc cctttatatt cattcatcag ctgagtgagc
     3121 cttactcttc tggatccgga aggttttcgt atatcgacaa agttactccc tacacattcc
     3181 aggtttcgga ttcgatcgtc aaaagcggca ttaataccta taaaataacc atctttttta
     3241 cgttctatat taaaaaattt caatcctaat tcaaaacgca ttatttctcc actttttatg
     3301 tctcccaaca accaggaatt agcgtaccct ccgttattct gtttcttcat gatagcgaca
     3361 aagtcatcca gcgagtttcc atattgcatg gctttacggg cccgataaaa ttcaggtgct
     3421 tcattttctt cataaacatt aaagcctcca atggttgtct ccacacccac aattcctgct
     3481 ccagtaataa aaaagtcggc aaaactatca ataaaaccag gtgaactttg cattaaga
//
LOCUS       DAOSOL010000145         3532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26607_length_3532_cov_3.283865, whole genome shotgun sequence.
ACCESSION   DAOSOL010000145 DAOSOL010000000
VERSION     DAOSOL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26607_length_3532_cov_3.283865"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..869
                     /locus_tag="PK171_04330"
     CDS             177..869
                     /locus_tag="PK171_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05450.1"
                     /translation="MSHPFGTNGRGQDLFWMMTFALKNSLIFGLEVAILSRLIAIIIG
                     VIAGYKGRIIDQMLIFLCDSFIVLPIFPILIFLKLLTREMDLSTLAIVLALFGWPWDA
                     RLIRSQVLSLREQKFTITAIFSGENTWELIFYEYFPHILPIVLATTINNMLWSLGFEI
                     TLSVLGLTSLDIPTMGTITYWAIQQQAMVMGIWWWIFIPIAFIILLFTSLYLLFVSMT
                     DFINPRARVLSR"
     gene            866..1864
                     /locus_tag="PK171_04335"
     CDS             866..1864
                     /locus_tag="PK171_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05451.1"
                     /translation="MSKVIKQEKQTQVLLEVANLKAYYTTEQYGIVKEIKAVEDVSIN
                     IEGNSFLAIVGESGCGKTTLARALYGAVDSTLSIIEGKVCYHFGDQKYELSPNGNDLK
                     NIWWERISYIPQGSLSALNPVRRLRDIFSDLANSHGKTFDEQKVKDHLEMVNLPSYVL
                     RIYPFELSGGMRQRSVIALATFLKPDVIIADEPTSALDVVTQRDILQLLSTIQNQSKS
                     TFIFITHDISLVPGLSNMMAIMYGGCIIEFGSTKSVFANPLHPYTKFLLSSIPKIGDK
                     TEKKSIPGNPVSLTNPPPGCRFCPRCPRGKEVCSDSRPELLNIDSQQHYVACWLYR"
     gene            1940..3430
                     /locus_tag="PK171_04340"
     CDS             1940..3430
                     /locus_tag="PK171_04340"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HOT05452.1"
                     /translation="MNGYVKVWKVKTGEINDLIYGHFIEHLGRCIYGGIYDKNSPKSD
                     ERGFRKDVLEAVKKIQCPILRWPGGNFVSAYHWQDGIGPLDKRPTRLNYIWGGLESNE
                     FGTDEFIEYCKEIGAESYFAIGLGTSTLDEALSWLEYTNLDTPTEYVDLRKKYGNSDP
                     YRVKYWGIGNEVYGDWQVGHSSANEYSEKLRQYALFMKGIDPSIKVIAVGADNPEWDL
                     TVLKHAGKVIDYISIHQYHGSNNYYDTVASAYYVEERLQLLDSLIKHLQLDHIKIALD
                     EWNVWYQVIPEVEIIEKKMIFLEEPYSLKDALFAAGVFFALHRKCDSVQMANLAQMVN
                     ALGMIKTNSQSIVLTPIYHVFDMFVNHTGRTRLGIEIAADRYSIKAKSFFEGQLSFQL
                     HNVPYLDGSATYDEKRHMMCLALGNYYLDKNLEVKVDLSDLDVTKDANCFELNSSDVM
                     DRNDFENPSRVKINSRRMTIPDRVFSLQIPAHSLSVFEFPLQTGIN"
BASE COUNT         1076 a          647 c          704 g         1105 t
ORIGIN      
        1 cgagattgac tcccatagca attttagata caacaaaaag acttttcaaa cacgatttac
       61 gatttagaat tcctctgctt attcttattc tcttgataat ttttgccatc atatctttat
      121 tttcccctta tgattttcgt gcatcctact atgctccaat caaccgccca ccttcattga
      181 gccatccgtt cggaaccaat ggtagagggc aagatctctt ctggatgatg acttttgcct
      241 taaaaaattc gcttattttc gggttagaag tggcaatttt atctcgttta attgccataa
      301 taatcggtgt tatagcagga tataaaggaa gaattattga tcaaatgtta atttttctct
      361 gtgattcatt cattgtttta cctatcttcc caatattgat atttttaaag cttctaacca
      421 gagaaatgga cctttcgact ctggctattg ttctagctct atttggttgg ccatgggatg
      481 cacgattaat tcgatctcaa gtactgagtt taagagagca aaagtttact ataaccgcta
      541 ttttctcagg tgaaaatact tgggaactga ttttctacga gtattttcct cacattcttc
      601 ccattgtgct ggcgaccacc ataaataata tgctttggtc ccttggtttt gaaataactc
      661 tatcggttct tggtctcacc tccttggaca ttccgacgat gggaaccata acctactggg
      721 ctatacagca gcaagccatg gtaatgggca tatggtggtg gatatttatc ccaattgcct
      781 ttattatcct tctttttact tcactatacc ttctttttgt gagcatgaca gactttatta
      841 atccacgggc cagggtgcta agcagatgag caaggtgatt aaacaagaga aacaaactca
      901 agtattatta gaagttgcta acctaaaagc ctattatact accgaacaat atggaatcgt
      961 gaaagagatt aaagcagttg aagatgtgtc aataaatatt gaaggaaata gttttttagc
     1021 gatagtcgga gaatctggct gcggcaaaac aaccttagcc agagcattat atggggctgt
     1081 cgattcaact ctatcgataa tagaaggaaa agtttgctat cattttggtg atcaaaaata
     1141 tgagctctct cctaacggca acgacctaaa gaatatttgg tgggaaagaa tttcttatat
     1201 ccctcaaggt tctttaagtg ctctcaatcc ggttagaaga ttaagagata tattttccga
     1261 cctggcaaac tcccatggaa aaacttttga cgaacaaaaa gtaaaagatc atttagaaat
     1321 ggtaaatctt ccttcttatg ttttacgcat ttaccccttt gaactttctg gaggaatgag
     1381 gcaaaggagc gtcatagcct tggctacttt tttaaaacca gatgtaatca ttgctgatga
     1441 accaacatcg gctctggatg ttgttactca acgtgatatt ttacaacttt taagcactat
     1501 tcagaatcaa tcaaaaagta catttatttt tattacccac gacatttcct tggttcctgg
     1561 tttatcaaat atgatggcaa tcatgtatgg tggttgtatt atagaatttg gctcaactaa
     1621 atcagttttt gccaatcccc ttcaccctta tacgaagttt ctactctcat caatacctaa
     1681 aattggtgat aaaacagaaa aaaaatcgat cccagggaac ccggttagtc ttactaatcc
     1741 acctcctggt tgtagatttt gcccaagatg ccctcgaggt aaagaagtgt gttccgattc
     1801 cagacctgaa ttgcttaata ttgattctca acagcactat gttgcatgtt ggttataccg
     1861 gtaatccctt tttttatcga cttatttatt cgtatatttt agatagattg aaagagaaat
     1921 aaactgggga gggaaggtaa tgaatggtta tgttaaagta tggaaagtga aaaccggtga
     1981 aataaatgat ctcatttatg gtcatttcat cgagcatttg ggccgatgta tttatggtgg
     2041 tatttatgat aagaattcac cgaagtctga tgagcgtgga tttcgaaaag atgttttaga
     2101 agctgtcaag aaaattcagt gtccaattct gagatggccc ggtggcaatt ttgtatcagc
     2161 ctatcactgg caagatggaa ttggaccact cgataaaagg ccgactcgtt taaattatat
     2221 ttggggaggt ttggaaagca acgaatttgg aaccgacgaa ttcattgaat actgtaagga
     2281 aattggagca gaatcttact tcgctattgg cttaggaacc agtacccttg atgaagcatt
     2341 gagttggtta gaatacacca atcttgacac tcctactgaa tacgttgatt tgcgcaaaaa
     2401 gtatgggaac tcggatcctt atcgagttaa gtattggggg atcggaaacg aggtttacgg
     2461 tgattggcaa gttggtcata gcagcgccaa tgaatattcc gaaaagttac ggcagtatgc
     2521 cctgttcatg aaaggtatcg atccttcaat aaaagtcata gcagtaggag ccgataatcc
     2581 ggaatgggac ctgacggtat tgaaacatgc cgggaaagta attgattata tttctataca
     2641 ccagtaccat ggctcgaata attactatga cacagtagct tctgcgtact atgttgaaga
     2701 acgactacaa cttttagata gcttaattaa gcatcttcaa cttgatcata tcaaaattgc
     2761 ccttgatgaa tggaatgtct ggtatcaggt tataccagaa gtcgagataa tagaaaaaaa
     2821 gatgatcttt cttgaagaac cttatagttt aaaagatgct ttgtttgctg ccggtgtatt
     2881 ttttgccctg caccgcaagt gtgattcggt tcaaatggcc aatctagctc aaatggtcaa
     2941 tgctttggga atgataaaaa ccaattccca aagcatagtt ttgacaccca tctatcatgt
     3001 ttttgacatg ttcgtgaacc atacgggccg aacgcgtctg ggtatagaaa tcgcagcaga
     3061 tagatattcg ataaaagcaa agagtttctt tgaagggcag ttatcttttc aacttcacaa
     3121 tgttccctat cttgatggat cggctaccta tgatgaaaaa cgccatatga tgtgtcttgc
     3181 tttaggaaat tactatttgg acaagaacct ggaggtcaag gtcgatcttt ctgatttaga
     3241 tgttaccaag gatgccaatt gcttcgagct taactcctca gacgtaatgg ataggaatga
     3301 ttttgaaaat ccgagccgag tgaagatcaa ttcaagaagg atgacaatcc cagaccgggt
     3361 gttttcactt caaattcctg ctcattcttt gtcggttttt gagtttcctc ttcaaactgg
     3421 cataaactga ctcgaaaggg aaaccaatga tggaaaaagg tatcaaaaag aaaaaaccga
     3481 tagaaatgaa aagtgtaaag atactcaatc aaaatacttt ttggggccaa cg
//
LOCUS       DAOSOL010000146         3528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26648_length_3528_cov_3.080046, whole genome shotgun sequence.
ACCESSION   DAOSOL010000146 DAOSOL010000000
VERSION     DAOSOL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26648_length_3528_cov_3.080046"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1849
                     /locus_tag="PK171_04345"
     CDS             140..1849
                     /locus_tag="PK171_04345"
                     /inference="COORDINATES: protein motif:HMM:NF027591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4919 domain-containing protein"
                     /protein_id="HOT05453.1"
                     /translation="MKIPFRMVKTIFMLILIWSYFFSQAIVYSQNLTPDEEYISIIEK
                     IKKGEDTETDYTKLRMLYTQTSFYQPESPERDENILKMAQAWFDKHNDEAIELGNQLL
                     EGEYLNPFVHDLFAKIYKESGEGDSLFHLLMFINIIASIQGDGKSYETAYQVISTWEE
                     KAMMTFVQVTPADQDQVFEPNLHDGHYFHVIQVKADETGEISDLYFNIDIPYQKAQQT
                     GPIMTTPVIIPPPPTPALQPITTYTDPTGQFSIHFPAGFSLLASQTNLIQCMAPNNGN
                     LYLIISDYANTMKAFSDEIIKRPGSLSGESQFQAGNLSGKIQLYTMSGSFQVLDGKSY
                     AVLLVTYAGIDYGLVVVLPTESYSAAQNWLSALVTGVQYQSVTSPPPAVVTPTVTPTF
                     PVTTSLEYTAPDGSFQVSLPAGSSKGGEWPNIVEYQTPSQGTLYILSGDTVGTLNLFR
                     QDLTQKQGKMLSQPVLFQTNEGDQGSMEVSTMVGSFQTADGKDYISFLVSYEQPEGAL
                     VIIVPNNLYTEAQGWISPLITGVTFQAVQPTPSPFLTPMPTPTATVTPTPLPTETLPP
                     LSD"
     gene            2061..>3528
                     /locus_tag="PK171_04350"
     CDS             2061..>3528
                     /locus_tag="PK171_04350"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT05454.1"
                     /translation="MNIEELSVKTIRMLALDTIQKANSGHPGLPLGAAPMAYAIFKKF
                     LSINPKNPCWVNRDRFVLSAGHGSALLYAMLYLSGFEKMTLEELKNFRQWESKTPGHP
                     EYDLECGVEITTGPLGQGFATGVGMAIAESHLAATYNRPGFNLVDHYVYAIVSDGDLM
                     EGVAAEAASLAGHLQLGKLIYLYDNNHISLASETGLHYSEDVRKRFEAYNWQVLEVKD
                     GNDLAAIEKAIQEAKADSTHPSLIMVTTHIGYGSPKQDSFEVHGSPLKPEEVVATKKF
                     YNWPEENPFFIPEDVLNHMRETVNKGSQAEKEWNALFAEYTKKYPAEAEELEKRMKGI
                     LPENWAQDIPSYPPETKNIATRSVGGEVMNAIAKNCPALMGGSADLDPSTNTVLKGKG
                     TFHPPVPEAEKMQGVVAGPFGYSGRNIMFGIREHAMGSILNGMALHGGIIPYGATFLT
                     FSDYMRPAIRLAALFNLHVIYLFTHDSIGLGEDGPTHQP"
BASE COUNT         1041 a          759 c          717 g         1011 t
ORIGIN      
        1 ctacattata tgaagatcgg gcgcaataaa ttgtcgcccc tacgtttttt taaaatattt
       61 tgtatagtct tggtttatcc atgctcatta gttttcagag taaattatct aaaaaaatag
      121 tggaggagag gaaagaaaga tgaaaatccc ctttcgaatg gttaagacta tattcatgtt
      181 aattctcata tggtcgtatt ttttttctca ggcaatagta tatagccaaa atctaacacc
      241 agatgaagag tatatctcaa ttatagaaaa aataaaaaag ggagaagata ctgagacaga
      301 ttataccaag cttcgaatgc tttacactca gacgtctttt tatcaaccgg agtcaccaga
      361 acgagatgag aatatactca aaatggctca ggcttggttc gataaacata atgatgaagc
      421 cattgaatta ggaaaccaac ttttagaagg agaatatctg aatccttttg ttcatgattt
      481 attcgcgaaa atttataaag aaagcgggga aggagattcc ttatttcatt tgttaatgtt
      541 tattaacatc attgcctcta ttcaaggaga tggaaaaagt tatgagaccg cctatcaggt
      601 catatcaact tgggaagaaa aggcaatgat gacctttgta caggtaactc cggcagatca
      661 agatcaagtt tttgaaccga atttacatga tggtcattac tttcatgtga ttcaggtcaa
      721 agccgatgaa accggtgaaa taagcgatct ttattttaat attgatatcc cataccaaaa
      781 agcccaacaa actggtccga ttatgaccac gccagttatc atcccacctc cccccacccc
      841 agctctccaa cccatcacta cctataccga tcccaccggt cagttttcaa ttcattttcc
      901 tgctggtttt tcccttctgg cttcacaaac caaccttata caatgtatgg cccccaataa
      961 tggcaatctc tatctgatca tctctgatta tgcgaatact atgaaagctt tttccgatga
     1021 aatcataaaa agaccgggat ctctctccgg ggaaagccag ttccaggctg gaaacctatc
     1081 gggaaaaatt cagctctata ctatgtctgg atcttttcag gtccttgatg gaaaaagtta
     1141 tgccgttctc ctcgtcacct atgctgggat agactatggt ctggtagtgg tacttcccac
     1201 cgaatcctat tcagctgctc aaaactggct ctccgccctg gtaactgggg ttcaatacca
     1261 atctgttacc tctccaccac cagcggttgt gaccccaact gtaactccca ctttcccggt
     1321 tacaacctct ctggaataca ctgcacccga tggatcattc caagtttcac ttcctgctgg
     1381 atcgagtaaa ggaggggagt ggccaaatat tgttgaatac caaactccca gccaagggac
     1441 tctctatatt ctcagtgggg acactgtcgg aactctgaat cttttccgtc aggatttgac
     1501 ccaaaaacag ggcaagatgc tgagccagcc ggtgcttttt caaaccaatg aaggagacca
     1561 aggaagcatg gaagttagta ccatggtagg aagctttcaa acagccgatg ggaaggacta
     1621 tattagtttt ctggtgagct atgaacaacc cgaaggtgcc ctggtgatta ttgttcccaa
     1681 caatctctat actgaagctc agggttggat ttcccccctc attaccggtg tgacttttca
     1741 ggctgttcaa ccaaccccat ctccctttct aacaccgatg ccgactccaa ccgcaacggt
     1801 aacacctact cctctcccaa ctgaaacctt accacctcta tccgattgat aggttattgt
     1861 tcaacgatat ttttcttgca tagctttttc atttttagtt tgtatggcat aataatgaga
     1921 ctggataacc atttattttg taacggcaaa tatttaaccg gcttcgaaaa acgaagcctc
     1981 aggatgacat aattttttac gttcctgtat tttcagaata tatcaagaaa ttaataattg
     2041 gaaggaaagg agatttattt atgaacattg aagaactttc ggttaaaacc atacgtatgc
     2101 tggctttgga taccattcaa aaagccaatt ctggtcatcc tggtctgcct ttaggagcag
     2161 ctcctatggc ctatgctata tttaaaaaat ttctctctat caaccccaaa aatccttgct
     2221 gggtgaatcg ggatcgtttc gttctttccg ctggtcatgg ttcagcactt ttatatgcaa
     2281 tgctttattt atcgggattt gaaaaaatga ccctggagga gttgaaaaac tttcgccaat
     2341 gggagagcaa aactcctgga catccggaat atgacttgga gtgtggggta gaaatcacca
     2401 ctggaccttt gggacaaggt tttgccaccg gggtagggat ggctattgcg gaaagtcatt
     2461 tagctgcaac atacaatcgc cctggtttta acctggttga ccactatgtc tatgctatcg
     2521 tgagtgatgg tgacctcatg gagggagttg ctgccgaagc agcttcattg gcaggtcatc
     2581 tgcaattagg gaaactcatt tacctctacg ataataacca tattagcttg gcatcggaaa
     2641 cagggcttca ttattccgag gatgttagaa aaagatttga agcctataat tggcaagtgt
     2701 tagaagttaa agatggtaat gatttggctg caatcgaaaa agccatccag gaggccaaag
     2761 ctgatagtac ccatccgagc ttaataatgg tcaccactca tatcggctat ggaagtccaa
     2821 aacaagatag ttttgaggtt catggttctc ccttaaagcc tgaagaagtg gtagctacca
     2881 aaaagtttta taactggccg gaagaaaatc ccttttttat acccgaggat gtcttaaatc
     2941 atatgagaga aaccgtcaat aaaggatctc aggcagaaaa agaatggaat gctttatttg
     3001 ctgaatatac caaaaaatat ccggctgaag ccgaagaact tgaaaaaaga atgaaaggaa
     3061 tccttcccga gaattgggca caggacattc cttcttatcc cccggaaacc aaaaacattg
     3121 ccactcgcag tgttggagga gaagtcatga atgctattgc taaaaattgc ccagctttga
     3181 tgggtggttc agctgacttg gatccttcta ccaacactgt tcttaaggga aaagggacct
     3241 tccatccacc agttcctgaa gcagaaaaaa tgcagggagt cgtggctggt ccttttggtt
     3301 actctggaag aaacataatg tttggtattc gagaacatgc catgggaagc attcttaacg
     3361 gaatggcact tcatggcggt atcattcctt atggtgctac ttttcttacc ttttccgact
     3421 atatgcgacc agctatacgt ttggcagcgc tatttaacct ccatgttatc tatttattta
     3481 ctcatgattc aatcgggctt ggcgaagatg ggccaactca tcagccag
//
LOCUS       DAOSOL010000147         3516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26756_length_3516_cov_4.577579, whole genome shotgun sequence.
ACCESSION   DAOSOL010000147 DAOSOL010000000
VERSION     DAOSOL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26756_length_3516_cov_4.577579"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PK171_04355"
     CDS             complement(<1..1089)
                     /locus_tag="PK171_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05455.1"
                     /translation="MTRELVMIDGNEATTYSAYHINEVIAIYPITPSSTMGELADQWA
                     AENKPNIWGTVPQVIEMQSEGGAAGALHGSLQAGALSTTFTASQGLLLMIPNMYKIAG
                     ELTPALINVAARTVATHALSIFGDHSDVMATRETGFALLSSNSPQEAMDLALIAQAAT
                     LESRVPFLHFFDGFRTSSEVAKVELLSIDDMKAMIDDRLVEDFRNRKLSPDRPFIRGT
                     AQNPDVFFQAREACNPYYLNCPSVVVKVMEKFEKLVGRKYRLFDYHGVPDAESVIVIM
                     GSGAETTQETVDYLNERGAKLGLIKTRLYRPFSVEHFIAALPKTVQTIVVLDRTKEPG
                     AVGEPMYIDVKTAIEEAVEKGMLSQSHKP"
     gene            complement(1206..1817)
                     /gene="recR"
                     /locus_tag="PK171_04360"
     CDS             complement(1206..1817)
                     /gene="recR"
                     /locus_tag="PK171_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT05456.1"
                     /translation="MSDSYPEALKNLIASFSRLPGVGPKTAQRLSFFLIKSLPDEIEG
                     LINSLTEVKQKIHFCSRCGFYTENELCSICQDSTRESTILCVVERPQDVLSIEKTGYR
                     GLYHVLQGVLSPLSGVGPEDLTINQLMKRIETEKFKEVILATNPSLDGEATAYFIARK
                     LRSFSIRVTMIARGLPLGGDMEFADEITLTQAFEGRRDVHIDT"
     gene            complement(1995..2285)
                     /locus_tag="PK171_04365"
     CDS             complement(1995..2285)
                     /locus_tag="PK171_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOT05457.1"
                     /translation="MKEAQKMQAKMAKLQEELKTKQVEASSGGGMVKVICNGQQEILE
                     IKIEPSLLEENDIEMLQDLVLAAVNEALNRSREMAQEEMAKITGGFNLPGLM"
     gene            complement(2257..>3516)
                     /locus_tag="PK171_04370"
     CDS             complement(2257..>3516)
                     /locus_tag="PK171_04370"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05458.1"
                     /translation="IFILATTEPHRLPDTILSRCLRINFNSIENEAMVQHLSKITNQE
                     GFSIEEAALYLIAQKSAGSLRDAVSFLEQLMSWEEKRITYEHATLVLREVDQSDLDAL
                     FVLLSKGDCGMVLLKTNEWILRGLEPEDIARSLSQYFHSLLILSLIESPSPSGIAPQR
                     IQILQEMVSPHPVLALRKILQRLQGLELEIRRSSHPQILLELALLDIIDLLANQQKSM
                     DLTTSPERALAKEMSVPNKKSPISYSSLTNTQSVNAFDDEWQTILQRLKKRKISLYAF
                     LQEAELREDSNSKLILAFGPNNRFHKESVERKENFQIIIDVLQEIRGKKCQLECVIDS
                     SNNSDESEQIPSDETDHVVPNNFNNDKILINKVENNNNQVKPFLAAVEIFGGLVVEYS
                     AIDDHLRIGWEETDFEKSDEGSPENAG"
BASE COUNT         1040 a          730 c          686 g         1060 t
ORIGIN      
        1 aggtttatga gattgagaaa gcatcccttt ctccactgct tcttcgatag ctgtctttac
       61 atcgatgtac attggttcac ccaccgctcc tggttctttg gttcggtcaa gcacgacaat
      121 ggtttgaacg gttttaggaa gagcagcaat aaaatgttct actgagaagg gacggtaaag
      181 tcgagttttg attaagccta atttagcccc gcgctcattc aggtaatcga ccgtttcttg
      241 ggttgtttcg gctcctgatc ccataatcac aataaccgat tcagcgtccg gaactccatg
      301 gtaatcgaaa agtcgatact ttcttccaac tagtttttca aacttttcca taacttttac
      361 gacaacactc gggcagttaa gataataagg attacatgct tcacgggctt gaaagaaaac
      421 atcagggttt tgagcggtac cccgaataaa cggacgatcg ggagaaagct tccggtttcg
      481 gaagtcttca accaaccggt catcaatcat tgctttcata tcatcaatag ataaaagttc
      541 aactttggca acctcggagg aggttcggaa cccgtcaaag aaatgaagaa aaggaacccg
      601 tgactctaaa gttgcagctt gggctattag ggctaaatcc attgcttctt gaggagaatt
      661 tgaagacaat aaggcaaaac cagtttccct ggtagccata acatcactat gatctccaaa
      721 aatagataaa gcatgagtag ctacggtacg agcagcaacg ttgataagtg ccggtgtaag
      781 ctctccagcg attttgtaca tgttgggaat cattaataat aacccttgag aggcggtgaa
      841 ggttgtgctt aaagccccgg cttgaagcga accatgaagt gctcccgctg cccctccttc
      901 gctctgcatt tcaattactt gaggaacagt tccccagata ttaggcttat tctcagctgc
      961 ccattgatcg gctaattctc ccattgttga agatggggtg atgggataaa tagcgattac
     1021 ttcgttgata tggtatgctg aataagtcgt cgcttcgttc ccatcgatca tgaccaattc
     1081 tcgtgtcata tcttatctca cctttcttat tggattattt cccaattcgt tttataggta
     1141 aaaaacattt ttcacgtgaa aattattgta acattattat tgtggtctca tcaaaatatt
     1201 gaaaatcaag tatctatgtg aacatccctt cttccttcaa aagcttgagt tagggtaatt
     1261 tcatcagcga actccatatc cccacctaag ggtaacccac gagcaatcat cgtgactcga
     1321 atcgaaaaag aacggagttt acgggcaatg aaataggcag ttgcttcacc gtcaagagaa
     1381 ggattggtgg ctaaaattac ttctttaaat ttttcagttt caatccgttt catgagttgg
     1441 ttgatggtca aatcttctgg accgacacct gaaagaggag aaagcacacc ctggagaaca
     1501 tgatagagac cacgataacc agttttttca atagaaagaa catcctgagg tcgttcaaca
     1561 acgcaaagta tggttgattc ccgggtagaa tcctgacata tagaacaaag ttcattttct
     1621 gtataaaaac cgcaacgaga acaaaagtga attttttgtt tcacctcagt aagggaatta
     1681 atcaacccct ctatctcatc cggtaatgat tttattagaa aaaaagataa cctttgagcg
     1741 gttttggggc ctacgccagg gagacgcgaa aatgaggcga ttaaattttt taatgcctct
     1801 ggataagaat cactcaaaca acttccacct cacatatagt taattgctta acaacttcca
     1861 cctcaaaatc tttaatccac cccgatatcc ctcttctgtg ggaaaaaaga cctaagggga
     1921 gataactcga gagggtgagg gggcgaaaga aaaagcaaaa actttccaga aaagaataat
     1981 ccacccctac actttcacat caacccaggg agattaaatc ctccggtaat tttcgccatt
     2041 tcttcttgag ccatctctcg agaacgattg agagcttcat taacggcagc caaaactaaa
     2101 tcttgaagca tttcaatgtc attttcttct aacagcgaag gctcaatttt tatttcaaga
     2161 atttcttgtt gaccattaca aattacttta accattccgc ccccgctgga tgcttcaact
     2221 tgtttggttt ttagttcttc ttgcaatttc gccattttag cctgcatttt ctgggcttcc
     2281 ttcatcagat ttttcaaagt cagtctcctc ccatccgatt cttaaatgat catcaatggc
     2341 cgaatattca acaaccaagc cgccaaaaat ctcgaccgcg gctaaaaagg gttttacctg
     2401 attattattg ttttcaactt tattaattaa aattttatca ttattaaaat tatttggaac
     2461 tacatgatca gtttcatcgc ttggtatttg ctctgattca tcagaattat tcgaactatc
     2521 aataacacac tccagttggc attttttgcc tctgatttcc tggagaacat cgataattat
     2581 ctggaagttt tcctttcttt ctacactttc tttgtgaaat cggttatttg gtccaaacgc
     2641 taaaattaac ttggaattgc tgtcctcccg caactcagct tcttgcaaaa aagcataaag
     2701 agatatcttt ctttttttta agcgctgtaa aatagtttgc cattcatcat cgaatgcatt
     2761 gacagattgg gtattcgtta acgatgagta agaaatcgga cttttcttat taggaacgga
     2821 catctccttg gcaagagctc tttcaggaga agtggttaaa tccattgact tttgttggtt
     2881 cgccagaaga tctattatat ctaatagggc caattcaagc aatatttgcg gatgagaaga
     2941 ccgtcttatt tctaattcca atccctggag gcgttgcaag atttttctta aggcaagtac
     3001 tggatgggga ctgaccattt cttgcaaaat ttgaatcctt tggggagcaa tgcctgatgg
     3061 agacggggat tcaatgagag atagaatgag gagagaatga aaatactgac ttagagaacg
     3121 agcgatgtct tcaggctcta atcccctcaa aatccactcg tttgttttta gcaacaccat
     3181 accgcagtct cctttactaa gaagaacaaa caaagcatcg agatcggatt ggtcaacttc
     3241 tctcaaaact aaagtagcat gttcataagt aattcttttt tcttcccagg acataagttg
     3301 ttctaggaag cttactgcat cacggagcga gccagcagat ttttgggcta ttaggtataa
     3361 agctgcctct tcaattgaaa agccttcttg attggtaatt ttgctaagat gctgaaccat
     3421 ggcttcattt tcgatagaat tgaagtttat tcgaagacat cgagataaga tagtgtcagg
     3481 taggcgatga ggttcagtgg tagccaaaat aaaaat
//
LOCUS       DAOSOL010000148         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_26826_length_3510_cov_3.971925, whole genome shotgun sequence.
ACCESSION   DAOSOL010000148 DAOSOL010000000
VERSION     DAOSOL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26826_length_3510_cov_3.971925"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PK171_04375"
     CDS             <1..247
                     /locus_tag="PK171_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05459.1"
                     /translation="LDKGVIIGEGSRIGGGQVLNVNKKYPEVLWSGITIIGKNSRIPS
                     GCEIGKNCIIGADLDENDFPSLIVLSGETIEKPSMEV"
     gene            250..1782
                     /locus_tag="PK171_04380"
     CDS             250..1782
                     /locus_tag="PK171_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05460.1"
                     /translation="MKKFSFLLMVLFFLVVGIQPALAQIYEFQVNLNEQTYLDLLPVS
                     PFVTVLPDKGNFATYFIGDPISLVYEALKTGYVSILDYTEDERVRILKNNEPVSPGTK
                     KSIQGIVTEPEGMERFLILLSPRVIPDRILVQAMNNPSRMKEVVGENIYLNRSIIQVV
                     EKRKEESVVIRFHSVPESILSGRNLRIRMVMTDKNNNPLVNRRVQWYASQGSLDFYQT
                     YTNTAGESEVWFMAPFVSEETQIQIQATYEGDTAYSPMDGTVTIIVQPEKKSSILALS
                     PKDFLVTSGESMSFQARLTDSDGNPLSGQALIWSSSVGQWEKRMTSTVFDGTSANLWI
                     APSSVVKTPVELKVNFRGAMYYSGFEDNSIGSVEGVEIVSGRGTYFIDFSSGKPETNF
                     EESIYRGENISGFTQNPASVLAMRSGEYLNVKFQAPEGWSNAACFIWGKSSGSVIIHV
                     HLNDNKVFTGNIIGGQIFPFEFQSFQLDQFLITGENQLRIEVQAPDGSAFFAIQRLLL
                     VF"
     gene            2158..2595
                     /locus_tag="PK171_04385"
     CDS             2158..2595
                     /locus_tag="PK171_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT05461.1"
                     /translation="MPFKENWWEEFDSMQKEMQRFMEHVSGKKPHFLGLSEKAWEPLC
                     DVFETDDNFIVVVDLAGVLVNEVDLTIQSRKLILKGVRKEVPSPPKRDYHQMEIPFGP
                     FQREIDLPEEVDGETINARYREGFLIVECKKKKVITERRIPLE"
     gene            2721..>3510
                     /locus_tag="PK171_04390"
     CDS             2721..>3510
                     /locus_tag="PK171_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOT05462.1"
                     /translation="MEGYIDNWFTLSEEPPEIKADQNVISESIDQEKKGFDIPEKIAV
                     LPLDENVLFPELVMPWVVHGEKWVKLVNDAVLGNKMIGVLTLRTKKEDGDNTVSAQDF
                     YDIGVVGRISRLLKLPEDAIQILVFGLNKFRVVEWIQWDPYPMAKIEIIKEEEIKTDE
                     TEALVRNILTLFQKVVELAPYLPKEIFVAAMNIKQPSRLAHFVISNLNLDVESKQNIL
                     STNELIEKLKKVNYYLTRELDILQIGSKIQTQIQSEMAKTQREYF"
BASE COUNT         1137 a          555 c          752 g         1066 t
ORIGIN      
        1 tttagacaaa ggagttatta tcggtgaagg atctcggatt ggagggggtc aagtattgaa
       61 tgtgaataaa aagtatcctg aggttttatg gagcggaata accattatcg gaaaaaattc
      121 ccgaattcca tcaggatgtg aaatagggaa aaattgcatt attggtgcag atttggatga
      181 aaatgatttc ccatcgttaa tagttcttag tggtgagacc atagaaaaac catcgatgga
      241 ggtttagtta tgaaaaaatt ttctttttta ctgatggttt tgtttttttt ggtggttggt
      301 attcaaccgg ctttggctca gatatatgaa tttcaagtca atttaaacga acaaacctat
      361 cttgacttgt taccagtttc gccatttgta accgtacttc ccgataaagg taactttgca
      421 acctacttta tcggcgatcc gatttcccta gtttatgaag cacttaaaac cggttatgtg
      481 agcatattgg attataccga agatgaaaga gtccggattt taaaaaataa cgaaccagtt
      541 tcaccgggta caaaaaaaag tattcaaggt attgttactg aaccagaagg aatggaaaga
      601 ttcctcattc ttctttcccc gcgggtcata cccgatcgta tcttggttca agccatgaac
      661 aatccatccc gaatgaagga agttgttggt gaaaatattt atctcaaccg gagtatcatt
      721 caggttgttg aaaagagaaa agaagagtcg gttgttattc gctttcattc cgtaccggaa
      781 tcaattttaa gcggaagaaa cttgagaatt cgaatggtca tgacagataa aaacaataac
      841 cctctggtta atagaagggt tcagtggtat gccagccagg ggagcttgga tttttaccag
      901 acctatacca atactgctgg agaatcagaa gtttggttta tggctccatt tgtttcggaa
      961 gaaactcaaa tccaaataca agctacttat gaaggtgaca ctgcctatag ccctatggac
     1021 gggaccgtaa ccatcatagt tcaaccagaa aagaaatcga gtattttagc tctatctcca
     1081 aaagattttt tagttacgag cggtgagagc atgagtttcc aagcccggtt aaccgatagt
     1141 gatgggaatc ctctttccgg acaagctttg atatggtctt ccagtgttgg tcaatgggaa
     1201 aaaagaatga cgtcaacagt atttgatgga acctcagcta atctttggat agcgccttct
     1261 tcagtggtaa aaacgccagt ggaattgaaa gtaaacttta gaggagcaat gtattatagt
     1321 ggatttgagg ataactcaat tggttcggtt gagggggtcg agattgtatc cggacggggt
     1381 acctatttta ttgatttttc aagcggaaaa cccgaaacga acttcgagga aagtatttat
     1441 cgtggagaaa atatctcggg attcactcaa aaccctgcct cggtccttgc catgaggtcg
     1501 ggcgaatatt tgaatgtaaa atttcaagct ccagaaggat ggagtaatgc agcttgcttt
     1561 atttggggaa aatcgagtgg aagtgtgatt attcatgttc atctcaacga taataaagtt
     1621 ttcacaggaa acattattgg cggtcagatt ttcccttttg aattccaatc ttttcaatta
     1681 gatcaatttt taattacagg agaaaatcaa ttgcgaattg aagtacaagc cccagatgga
     1741 agtgccttct ttgccatcca acgtttatta ctggtttttt agaatttatg aagttggtat
     1801 tgaatgagta tcgataaaat ttcatctcca aaaacgttga tattattttg tagttataga
     1861 tgagagtgaa aacctggcaa ttggcatggt tctttattat ttgggagtaa taaattgcac
     1921 cccgacaatt ttttgctttt tgtagaagtt tgattttcaa aacagcaagc ggtttgtaga
     1981 aaagaatccc aagccacttg tgtagtacca gatgaggatg agggtgaaag cccagaatga
     2041 gattctggtt ggttgctata attgagttaa ctataaaaac gtcatggatt tttagagtgg
     2101 aataatttaa aagaatattt ttaattacac cgaaaagtag ggtaggtggg taatataatg
     2161 ccattcaaag agaattggtg ggaagagttt gattcgatgc agaaagaaat gcagcgcttt
     2221 atggagcatg tttctggaaa gaaacctcat ttcttaggac tttctgaaaa agcttgggaa
     2281 cctttatgtg atgtttttga aaccgatgat aattttattg ttgtggtaga tctcgccgga
     2341 gtattggtca atgaagtcga cttgactatt caatcacgaa aattaatact taagggtgtg
     2401 agaaaggaag ttccttcacc ccccaaaagg gattatcatc aaatggaaat tccctttggt
     2461 ccttttcaac gagaaattga tcttcctgaa gaggttgatg gagagaccat caatgcccga
     2521 tatcgggaag ggttcctcat agttgagtgc aaaaagaaaa aagttattac cgaacggagg
     2581 attcctcttg agtaagtggg tgctctcaaa aataaaaaat atcggtttaa aaagagaatt
     2641 ttctcaaaaa tataaagttc attataaata tttcaaccca tttcttgata agaatattgt
     2701 ttattaggaa aggatccgac atggaaggat atattgacaa ctggtttaca ctttcggaag
     2761 agcctccaga gattaaagct gaccaaaatg tgatttccga atccattgat caggaaaaaa
     2821 aaggttttga tattccggaa aaaattgccg tacttccttt agatgaaaat gtattatttc
     2881 ctgagctggt catgccttgg gtagttcatg gagaaaagtg ggttaaatta gttaacgatg
     2941 cggttttagg aaataaaatg attggagttt taacactccg taccaaaaaa gaagatggag
     3001 ataacaccgt ttcagctcag gatttttatg atatcggtgt tgtagggcga atatcccgtt
     3061 tattaaagct cccagaagat gctattcaaa tattggtgtt tggtttaaat aagtttcgcg
     3121 ttgttgaatg gattcaatgg gatccatacc ctatggcaaa aattgaaata ataaaagaag
     3181 aagaaattaa aactgatgaa actgaagctt tagtccgcaa catattaacc ctttttcaga
     3241 aggtcgttga acttgctcca tatctcccca aagaaatttt tgttgctgcg atgaatatta
     3301 aacaaccctc acgacttgcc cattttgtaa tttcgaacct gaatctggat gtagaaagca
     3361 agcaaaatat tttgtcaacc aacgaactta ttgagaaatt aaaaaaagta aattattatt
     3421 taactcgaga attggatatt ctccaaattg gcagcaagat tcagactcaa attcaatcag
     3481 agatggcaaa aactcaaaga gaatatttct
//
LOCUS       DAOSOL010000149         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_27042_length_3488_cov_3.671716, whole genome shotgun sequence.
ACCESSION   DAOSOL010000149 DAOSOL010000000
VERSION     DAOSOL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27042_length_3488_cov_3.671716"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PK171_04395"
     CDS             complement(<1..1663)
                     /locus_tag="PK171_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOT05463.1"
                     /translation="MPDIIITIGGEAGQGVQTIGEVLSRILIRSGFYVFSIQDYHSRI
                     RGGHNSVQIRFSDQPIQAIGTDLDLLVCLNSETQEIHQPALKSDGIMIGMIKKSPSTS
                     SAISINFNEMAEELGNRIFANIIAVGALIEILGIDDKIAELYLEEIFQKKGNDVVRLN
                     KEALALGQKEIKRQQIPRQFLDKKNGNPNQGNLLLIGGNEALGLGAILAGCTFYSAYP
                     MTPSTGVMNFISSRSKKYGIIVEQAEDEIAAINMAIGASYAGAKAMTATSGGGFCLMA
                     EGLGLAAMTETPIVVLDGMRPGPSTGLPTRTSQGDLEFVLSASHDEFPRFVFAPRDPQ
                     DAIDTMIRAFNLSDRFQVPVIILSDQYLADSSWTFSQMKMNEQPQKIQTVIGDHSYQR
                     YALTDSGISPRALPGMSEALVVADSDEHDEIGHLTEDLNQRVQMHQKRMKKIEKMKKE
                     TQPPVHIVGQTATIIGWGSTYGVLFEAREKLAKLGIEAGLLHFNDIYPLSTDTLTILK
                     SVPKPIVVEGNFQGQLARLLERETKTSFDLRVNRYDGRPFFVNELV"
     gene            complement(1701..2399)
                     /locus_tag="PK171_04400"
     CDS             complement(1701..2399)
                     /locus_tag="PK171_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05464.1"
                     /translation="MKTFGIIGCLLLVIGCFSLSVSALSIRGDVLWNDQPLSFLLITE
                     NSRYVLKIQNQHQQLMRVLADMEAEELLIIDDMMKEYASVHSKQLISIVSLLALGKVI
                     GVEESWLQGEQVNFFQTGEKKLYDPFGECLQLRVNDTITGMFWSFQLDNLFLPIITPM
                     LQKLTHDGIRFPFWESTVKMKGFIVANQKDQKTIYRLIEIGEVPLEWQKEIQHSDYEQ
                     KEWVDFLSPFKGEN"
     gene            complement(2550..>3488)
                     /locus_tag="PK171_04405"
     CDS             complement(2550..>3488)
                     /locus_tag="PK171_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT05465.1"
                     /translation="DGETCVIGGVLEHIEEAGVHSGDSACVIPPFSLSDEIVDQIKLF
                     TQALARELNVIGLMNVQYAVKEDTVYVLEVNPRASRTIPFVSKATGVPLARLASQVMV
                     GRKLSEIGLNREVEIRHFAVKEAVFPFNRFPGVDPILGPEMRSTGEVMGIDADFGMAF
                     AKSQIGTQSQLPQAGRVFISVKNQDKRGVIFVAKKLVDLGFQIVATKGTAKVLMNNGI
                     TVEIVNKVGEGRPNVVDFIKNQKIDLIINTPSGGRTQVESSFIRKEAVIKSINYVTTL
                     SGAEATVYAIERLKKFPIQVRSIQEYHQEILRTLPV"
BASE COUNT         1038 a          757 c          628 g         1065 t
ORIGIN      
        1 ccaccaactc gtttacaaaa aagggccttc cgtcataacg gttaacgcga agatcaaatg
       61 aagttttcgt ttctctttcc aataaccttg ccagttgacc ttggaaattt ccctccacaa
      121 caatcggttt aggaactgat ttcaaaatcg taagagtatc tgttgataaa ggataaatat
      181 cgttaaaatg aagtaatcca gcctcaattc ccagctttgc cagcttttct cttgcttcaa
      241 acaatactcc gtaagttgaa ccccatccga ttatcgtggc ggtttggccg actatatgaa
      301 ctgggggttg ggtttctttt ttcatctttt caattttttt cattcttttt tgatgcattt
      361 gaaccctttg gtttaaatct tcagtcaaat ggccaatctc gtcgtgttca tcgctatcgg
      421 caactactaa agcctcactc atcccgggaa gagcgcgtgg agagattcca gaatcggtaa
      481 gagcataccg ctggtaagag tggtcaccaa taacagtttg tattttctgg ggttgctcat
      541 tcattttcat ttgagaaaat gtccaactac tatcggctag gtattgatcg gaaagaatta
      601 ttaccggaac ttgaaaacgg tcggatagat taaaagcacg aatcattgta tcgatagcat
      661 cttgggggtc tcgaggagca aatacaaatc gaggaaattc atcgtgggaa gctgagagga
      721 caaattcaag atcaccctgg gaggttcggg ttggtaaacc ggttgaagga ccggggcgca
      781 taccatctaa aacaactatt ggagtttcgg tcatagccgc caatcctaaa ccttctgcca
      841 ttaaacaaaa acctcctcct gaagtggctg tcatcgcctt agcaccagca taggatgctc
      901 caatggccat attaattgct gcaatttcat cttcagcttg ttcgacaata ataccgtatt
      961 ttttagagcg ggatgatatg aaattcatga ctccggttga tggtgtcatg ggataggcgc
     1021 tataaaaagt acatcctgcc aggatcgcac ccagacctaa agcttcattt cctcctatta
     1081 ataaaagatt tccctggttc ggatttccat tttttttatc taaaaattgc cgagggattt
     1141 gttgtctctt gatttccttt tgtcctaaag caagtgcttc tttattgagc cgaacgacat
     1201 cattaccttt tttttggaaa atttcctcca aatacaattc ggcaatttta tcatcgatcc
     1261 ccaaaatttc aattaaagca cctactgcaa taatattggc aaatatccga tttcctaatt
     1321 cttctgccat ttcgttaaaa ttaattgaga tcgctgaaga agttgaaggt gattttttaa
     1381 tcatccctat cattatccca tccgatttta gtgctggttg gtgtatttcc tgagtttcag
     1441 agttgagaca tactaaaagg tctaaatcgg taccaatggc ttgtatcggt tgatcagaga
     1501 agcgaatttg aaccgaattg tgcccaccac gaatgcggga atgatagtct tgaatagaaa
     1561 aaacataaaa accacttcgg atgagaatac gcgataatac ctcacctatg gtttgaacac
     1621 cctgccctgc ttcgcctcct atggtaatga ttatatctgg catgtcatcc ctcctccctt
     1681 tcagcagtta gagaattcgc ttaattctct cctttaaatg gagatagaaa atccacccat
     1741 tctttttgct catagtcact atgttgaatt tccttttgcc attcaagggg aacttcccca
     1801 atctcaatta agcgataaat ggttttttgg tccttttggt tagctacaat aaaacctttc
     1861 attttgacag ttgactccca aaaagggaag cggataccat catgagtgag cttctgaagc
     1921 atgggggtta ttatgggtag aaataaatta tccagttgga aggaccaaaa cattccagtg
     1981 atggtatcat tcacccgaag ttgaagacat tcaccaaagg ggtcgtataa ttttttctca
     2041 ccagtttgaa aaaaattaac ctgttcacct tgtaaccagc tttcctctac accaatgact
     2101 tttcccaaag ccaacaaaga tacaatagag attaattgtt tcgaatgaac cgaagcgtat
     2161 tccttcatca tatcatcaat aataagtaat tcttcagctt ccatgtcagc caaaaccctc
     2221 ataagctgtt ggtgctgatt ttgtatcttt aatacataac gggaattttc agtaatgagc
     2281 aaaaaagata aaggctggtc attccataaa acatcccctc taatcgaaag ggctgaaacg
     2341 gagagagaaa agcaaccaat tactaggaga agacacccaa tgataccaaa ggttttcaca
     2401 ccacacctcc tcaaattgat aaaatcagtt taccataatc agaggaaaat ttttataatt
     2461 tcctaccttc agcaaaaata cctccctatc gattatgaat cgattgaagt cattgtaaaa
     2521 aaggattctt tttaatttaa tttgccaatc tatactggta gggttcttaa aatttcttga
     2581 tgatattctt gaatagaacg aacctgaatg ggaaattttt taagccgctc aatagcatag
     2641 actgtagctt cggctccgga aagcgtggta acatagttga tgctttttat tacagcctcc
     2701 tttcgaataa agctactctc cacctgggtt cgtcctcccg agggggtatt gatgatcaag
     2761 tcaatttttt ggttttttat aaaatccaca acattggggc ggccttctcc aaccttattc
     2821 acaatttcaa ctgtaatacc attattcatt aatactttag cagttccttt ggtggcaaca
     2881 atttggaatc caaggtcaac cagtttcttg gctacaaaaa taactcctct tttatcttga
     2941 tttttaacac taataaacac cctccccgct tgaggaagtt gggattgagt tccaatttgt
     3001 gacttggcga aagccattcc aaaatcagca tcaataccca tcacttcacc cgttgaacgc
     3061 atctcaggac ctaatattgg gtcaacacca ggaaatcgat taaaagggaa cactgcttct
     3121 ttaaccgcaa aatgtcggat ttcaacttcg cgattcaggc caatttccga taatttccga
     3181 ccaaccataa cctgggatgc taatcgagct aaaggaactc ctgtggcttt gctaacaaaa
     3241 ggtattgttc tcgaagcccg tgggttaacc tctaatacat aaaccgtatc ttctttaact
     3301 gcatattgta cattcataag tccaataaca ttcagttccc gagccaaagc ttgagtaaat
     3361 agcttaattt gatcaacaat ttcatcactg agagaaaagg gagggatcac acaagcacta
     3421 tctcccgaat gtactcctgc ttcttcaata tgttcgagaa ctcccccaat aacacatgtt
     3481 tctccatc
//
LOCUS       DAOSOL010000150         3477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_27143_length_3477_cov_3.935418, whole genome shotgun sequence.
ACCESSION   DAOSOL010000150 DAOSOL010000000
VERSION     DAOSOL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27143_length_3477_cov_3.935418"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /gene="purM"
                     /locus_tag="PK171_04410"
     CDS             complement(<1..585)
                     /gene="purM"
                     /locus_tag="PK171_04410"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT05466.1"
                     /translation="MTEGWTYQKAGVNVDLANTTVEKIKTLVKSTRRPEVITDIGGFS
                     GVFCMGEKGKNHPCLVASTDGVGTKLKIAIHLGVHDSIGIDLVAMCVNDILCTGAEPW
                     FFLDYFACGKLHSNILERVISGVVQGCQLAGCALLGGETAEMPGMYQDNDYDLAGFAV
                     GSVLEDHLIDGHSIQNGDGVIALASSGLHSNGFSL"
     gene            complement(582..1895)
                     /gene="purB"
                     /locus_tag="PK171_04415"
     CDS             complement(582..1895)
                     /gene="purB"
                     /locus_tag="PK171_04415"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT05467.1"
                     /translation="MIERYSRQEMSKIWGEEHRAQVWLKVEMFALEAWVKLGLLSQDE
                     YKKITNQVTLSLDRMKAIKNITRHDVIAFLTSLTEQCGLESRFIHFGMTSSDMLDTAQ
                     AFLMKEAADLLLEDCRKALSSIKQKAIKYRNTLMVGRTHGIHAEPTTFGLKMAMWYTE
                     MQRNYHRLIEARENIRVGKISGAVGNYANIPPFVEQYVCEKLGLKPAEVSTQIIQRDR
                     YAELVWAMAMIGTSLEKFALELRSLQRTEIREVEEGFSSGQKGSSAMPHKKNPITGEQ
                     ICGLSRVLRGNLIVAMENIPLWHERDISHSSAERIIIPDCCILCDYLLITFCRLLDNL
                     RIFPENMKKNLESNRGLVFSERILLELIKKGLSREDAYKIVQRSALTTWEDPQTSFQD
                     ILLQDDELKKYLSKEEIKKLFDYQHFLHSVDYIFKNAGLNEEDQE"
     gene            complement(1892..2212)
                     /locus_tag="PK171_04420"
     CDS             complement(1892..2212)
                     /locus_tag="PK171_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05468.1"
                     /translation="MKNYWNIFRLKIHLRSLAGEKDQGFLSLGKKVFQLLHNKELERE
                     DLKEDVQEILSIEDEIDQVREALYREMGQPPRKQCQSCGKYIDAQARYCPRCGNIQER
                     SKSE"
     gene            2355..2804
                     /locus_tag="PK171_04425"
     CDS             2355..2804
                     /locus_tag="PK171_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT05469.1"
                     /translation="MKISHILVPTDFSPFSDLAIQNAAFFCKVFDAQLTLAHVLTLSE
                     VNALTSEPGNPWENVIQAIQNDMQQEYNKNSSECQLGKNPILEVLVGEPAEEIVKFAE
                     ENQVNLIIMGTHGRTGLASIFLGSVTVDVIKKTCIPVTVIKCLSALH"
     gene            complement(2856..>3477)
                     /locus_tag="PK171_04430"
     CDS             complement(2856..>3477)
                     /locus_tag="PK171_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT05470.1"
                     /translation="NPDLTVIAESGDGCMYGEGGNHLIHAIRRNPNITVIVHNNQVYG
                     LTKGQASPTSEVGFVSKTQPLGTVSESFHPLALAVSQGASFVARGFTGDKKELKSIFK
                     QAIQWKGFSLVDVLQPCVTFNRTNTFGWYKSRVYSLPEEYDPEDQVGAFKKSLEWGDK
                     IPTGVIYRHAKPVFLDQVFEKLGRKEFLIPTNNYQVIFEDEKRRFE"
BASE COUNT         1037 a          762 c          612 g         1066 t
ORIGIN      
        1 taaagaaaat ccattgctat gaagacctga tgaggctaaa gcaataaccc catccccatt
       61 ttgaatacta tgtccatcaa tcaggtgatc ttctaaaacc gatccgaccg caaatccagc
      121 taagtcatag tcattatctt ggtacatacc cggcatttca gcggtctctc cccccaataa
      181 agcacaccca gctaattggc acccttgcac aactcctgaa ataactcttt ccagaatatt
      241 cgaatgcaat tttccacaag caaaataatc aagaaaaaac cagggttcgg caccagtaca
      301 aagaatatcg ttcacacaca tagctaccag atcgatacca atagaatcgt gaacccccag
      361 atgaatagca atctttaatt tggtcccgac accgtcggta ctggcaacta aacaaggatg
      421 gtttttccct ttttctccca tacaaaaaac tccagaaaaa cctccgatat cggtaataac
      481 ctcgggcctt cgggtagact ttaccaaggt ttttattttt tcaactgtcg tattggctaa
      541 atcaacattt acaccggctt tttgataagt ccatccttca gtcactcttg atcctcctca
      601 tttaaaccag catttttaaa aatataatca actgaatgaa gaaaatgctg gtagtcaaaa
      661 agctttttga tttcttcttt agaaagatac ttcttcaatt catcgtcttg aagtaaaata
      721 tcctgaaatg aggtttgggg atcttcccat gttgtcaacg ctgaacgctg aacaatttta
      781 taagcatcct ctcgacttaa ccccttctta attaattcaa ggagaatccg ttccgaaaaa
      841 accaaaccac gattggactc taaattcttt ttcatattct cggggaaaat tcgtaaatta
      901 tccaataatc gacaaaatgt aataagtaaa taatcacaga gtatacaaca atcgggaata
      961 atgatgcgtt ctgctgaaga atgagaaata tcccgttcat gccaaagtgg aatattctcc
     1021 atcgcaacta ttagatttcc tcttaaaact cgacttaaac cacatatttg ttctccggtg
     1081 atgggatttt ttttatgggg catagccgat gatccttttt gtcctgaaga aaagccttct
     1141 tcaacttctc ggatctcggt tctttgcaag cttcttagct ccaaagcaaa cttttccaag
     1201 gatgtcccaa tcatggccat tgcccaaacc aattcggcat aacggtctct ttggataatc
     1261 tgggtagaaa cttccgccgg ttttaaaccc aatttttcac aaacatattg ttctacaaag
     1321 ggtggaatat tcgcataatt ccctactgct ccagaaattt ttccaaccct tatattttca
     1381 cgggcttcaa ttaatcgatg gtaatttctt tgcatttcgg tataccacat ggccattttt
     1441 agcccaaagg tggttggttc agcgtgaatc ccatgggtcc gtcccaccat gagagtgttt
     1501 cgatacttta ttgctttttg tttgatggaa ctgagagcct ttcggcagtc ttcaagaagt
     1561 aaatcggctg cttctttcat tagaaatgct tgggcagtat ccagcatatc ggatgatgtc
     1621 ataccaaaat gaataaaacg ggattctaaa ccgcattgtt cggttaaact agtaagaaag
     1681 gcaatgacat cgtggcgagt gatgttttta attgctttca tacgatcaag agagagagta
     1741 acctgattag taatcttttt atattcatcc tgagacaata atcctagttt aacccaagct
     1801 tccaaagcga acatttcaac ttttaaccaa acttgagccc ggtgttcttc gccccaaatt
     1861 ttactcattt cctgtcgact gtaacgttca atcattctga cttactcctt tcttgaatat
     1921 ttccacaacg gggacaatat cgtgcttggg catcgatata tttcccgcaa gactggcatt
     1981 gtttacgagg tggctgtccc atttctcggt atagtgcttc tcgaacttga tcgatttcat
     2041 cttcaatcga aagaatctcc tggacgtctt cttttagatc ttctctttcc agttctttat
     2101 tgtgaagtag ttggaacact tttttcccaa gacttagaaa tccctgatct ttttctccgg
     2161 caagagagcg aagatgaatt tttagtcgaa agatattcca ataatttttc aaagcctctc
     2221 cctccagcat cataatcact agctttattt tgcttgtcat tttagcatat ttttatccac
     2281 ttcccggaag aaatatcttg cattttctct gggtaaaggt taatctgttg gaaaaaagag
     2341 ggagggaaca ttatttgaaa atctctcaca ttttggtacc aactgatttt tccccattct
     2401 ctgatctggc aattcaaaat gcggctttct tttgcaaagt tttcgatgcc caacttactt
     2461 tagcccatgt ccttaccttg agtgaagtga atgcactcac ctcagaacct ggaaatccct
     2521 gggaaaacgt tatccaagct atccaaaacg atatgcaaca agaatataat aaaaactcat
     2581 ctgaatgcca actaggaaaa aatccaattt tagaagttct ggttggagaa ccagccgaag
     2641 aaatcgttaa gtttgccgaa gaaaaccagg ttaacttgat tattatgggg actcacggaa
     2701 gaactggact tgccagcata ttcttgggaa gtgttacggt tgatgttatt aaaaagactt
     2761 gtattccagt tacggttatt aagtgtctat cggctcttca ttgagaaata atggttacgg
     2821 ggagattcct atctccccgt aggaaggaac tgcttttact caaatctacg tttttcatcc
     2881 tcaaaaatta cctgataatt gtttgtcggg attaaaaact ccttccgacc taacttttca
     2941 aaaacttgat ctaaaaatac cggcttggca tgacgataga taacaccggt gggaatcttg
     3001 tctccccact ccagagattt tttaaacgca ccgacttgat cttcgggatc atactcttcg
     3061 ggaagagaat acactcggct tttataccat ccaaaagtat tggtacgatt aaaagtgaca
     3121 caaggttgaa gaacgtccac caaagaaaat cccttccatt ggatggcttg tttaaaaatt
     3181 gactttaatt cttttttatc accggtaaat ccacgagcga caaagctagc tccttgagat
     3241 acagccagag ctaaggggtg gaaagattcg gaaactgttc caagaggctg ggttttgcta
     3301 acaaaaccaa cctctgaagt cggagaggct tgtcctttag tcaaaccata aacctgatta
     3361 ttatgaacaa tgactgtaat attggggttt cttcggatag catggataag gtggttacca
     3421 ccttcaccat acatacaacc atcaccagat tcagctataa cagtaagatc tgggttt
//
LOCUS       DAOSOL010000151         3456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_27373_length_3456_cov_3.373420, whole genome shotgun sequence.
ACCESSION   DAOSOL010000151 DAOSOL010000000
VERSION     DAOSOL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27373_length_3456_cov_3.373420"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..860
                     /locus_tag="PK171_04435"
     CDS             135..860
                     /locus_tag="PK171_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05471.1"
                     /translation="MVSELTKIYRTGSVEVIALKDVSFQVNKDEFIAIMGPSGSGKST
                     LMYILGCLDRATRGSYYFEGQEVSRLKENTLAIIRNKKIGFVFQTYNLLPRLNAFQNV
                     ELPLIYAGISGSQRKIIVKNALIQVGLENRIFHRPNQLSGGESQRVGIARALVNDPTI
                     LLADEPTGNLDSKSGEEVMQIFQNLNDQGKMIILVTHEREIAQHTKRILHFLDGKLVG
                     DEPVENPLRASDQLKQISTSVLC"
     gene            complement(914..2047)
                     /locus_tag="PK171_04440"
     CDS             complement(914..2047)
                     /locus_tag="PK171_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05472.1"
                     /translation="MNKKIIPAVIFYTCCFSLFFFFTLLAREKGLITSEVDFYSNQSQ
                     IFFSSKSEQPKLLFLSYPLLPFFFALIGYLPALIPPVIGTFTIWLLSKIIIQKNFPPI
                     FILLIITSPFFLLSLSSPSFFLLAFFITTTIVNIVQYHETQSIYFFFLAGISLGIGTL
                     TQLSFLWVIIPVTIFQLFFFRDSLIRKTSLFLVSLFPPVFFLTAILFFNWVYSGNPFL
                     FLNTSPLNLRYMLPEFLISSSYNLPVFFSKLWFIYPFLGLIFFQGKVRIFWMISVIIL
                     SFILFQNQLLFLPISFLTIIGLLLWNKKSGYQLMVGIIVFLSISGLGWYFFLNHQDSY
                     FPAQGSYQKTLTPNQPIIQKTAHSDSLFFIHFGPDTFLSSSCE"
     gene            complement(2049..>3456)
                     /locus_tag="PK171_04445"
     CDS             complement(2049..>3456)
                     /locus_tag="PK171_04445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT05473.1"
                     /translation="FLQEATSGLFYRSPEESAMRVFIPVQWISLGALCALILIGVFTL
                     PRLTILILLGLANLFYAISVIFRFVLSSSGAKLDTNSSLFKKNEPQLRPNDLPTYSIL
                     LPLYHEPPNVIRQLTTAIQNLDYPPEKLDVIFLIEEDDQDTLIHCKAEKPPYNVRFVI
                     VPEGMPKTKPRACNFGLAFARGEFLTIYDAEDIPERDQLKKAVACFRSSPPDIMCFQN
                     ALNFYNAEQNILTRLFTLEYSYWFDYLLPGLYLNHLPIPLGGTSNHFRTKILRDLGGW
                     DPYNVTEDADLGMRASYRGYRVGILPSTTFEEANSRVKNWIRQRSRWLKGYLQTFLVH
                     NRHPFQVVRNSGWKGWFTLQVFIGGTTLGQAFSLILWILFFIWLFNRGEFLQSYFSGP
                     ILYLGVFNLIVGNFLGIYLSMLAVFRRGIDRLIFYSLLNPLYWWLTSIAAWKGIFQLF
                     TRPFFWEKTIHGLQIKEK"
BASE COUNT         1289 a          547 c          760 g          860 t
ORIGIN      
        1 aaataaagac cgagagggga gggagcgagg gggagaggaa aaaagagaaa cgatagaagg
       61 aaaagatagg aatgaaaacc taaaggtctt tacaggtaag aaaggaggag ggaaagattg
      121 agaaaccaat aataatggtt tcagaattaa ctaagatata tcgaactgga tcagttgagg
      181 tcattgctct aaaagatgta agtttccaag ttaataaaga tgaatttatt gcgataatgg
      241 gaccatctgg ttcaggaaaa tcaaccttga tgtatattct tgggtgttta gatcgagcaa
      301 ccagaggaag ttattatttt gaaggccaag aagtatcccg attaaaagaa aatactttag
      361 ctatcatacg aaataaaaaa attggattcg tttttcagac ctataacctg ctacctcgcc
      421 tcaatgcttt tcagaatgtg gagctcccat taatctatgc tggaatctcc ggttcacaaa
      481 ggaaaataat tgttaaaaat gcacttatcc aagttgggtt ggaaaaccgg atattccatc
      541 ggccaaatca actttcaggt ggggaatctc aacgggtagg cattgctcga gcactggtaa
      601 atgacccaac tattttattg gcggatgaac cgactgggaa tctcgatagt aaatcaggcg
      661 aagaagtgat gcaaattttt cagaacttga acgatcaagg gaaaatgatt attttagtta
      721 ctcacgaaag agaaatcgca caacatacta agcgaatcct tcattttctt gacggtaagt
      781 tggttgggga tgaaccagtc gaaaatccac tccgtgccag tgaccaatta aagcagatat
      841 caacatctgt cttatgttag gtaaagaata ttcacgaaaa ccttatatcg attattcaca
      901 aaccctaaaa tagtcattca cagcttgatg aaagaaaagt atctggacca aaatggatga
      961 agaacaagga atccgaatgg gcagtttttt gtattatcgg ttggttagga gtaagggtct
     1021 tttggtatga gccttgagct ggaaaatagg aatcctgatg gtttaaaaag aaataccatc
     1081 ctaaacccga aatagataga aatacgataa tgccaaccat aagctggtaa ccactttttt
     1141 tattccaaag aaggagacca attatagtca aaaatgaaat cggaagaaac agcagctgat
     1201 tttgaaaaag gatgaaagag aggatgataa cgctaatcat ccaaaaaatt ctaactttac
     1261 cttgaaaaaa gatcagtcct agaaaaggat aaatgaacca gagttttgaa aaaaacactg
     1321 gtagattata ggaagatgag attagaaatt ctgggagcat atatctcagg ttcaatggag
     1381 aagtattgag aaataaaaag ggattaccgg aataaaccca attaaaaaat aggatagcgg
     1441 taagaaaaaa aactggaggg aagagtgata ccaagaaaag ggaagttttt cttataagtg
     1501 agtcacggaa aaaaaataat tggaaaatgg tcaccgggat aatgacccaa agaaaggata
     1561 attgagttaa ggttccaatt ccaaggctta tccctgctag aaagaaaaaa taaattgact
     1621 gggtttcatg atattgaacg atattaacga tggtagtagt gatgaaaaac gccaacaaga
     1681 aaaatgaagg cgaggaaaga gacaaaagaa aaaaaggaga agttataatc aagagaataa
     1741 aaattggtgg aaagtttttt tgaatgatta ttttggaaag tagccagata gtaaaggttc
     1801 caataaccgg aggaatgaga gcaggaagat aaccgatcaa agcaaagaaa aaaggtaaga
     1861 gaggatagga aagaaagagt aatttaggtt gttcggactt agaagaaaaa aagatttgtg
     1921 actgattgct gtaaaaatca acttctgacg ttatcaatcc tttttctcgt gctaaaagag
     1981 taaagaaaaa aaagagagaa aaacaacatg tatagaaaat aacggcagga atgatttttt
     2041 tattcatcct atttttcctt gatctgtaat ccatgaatag ttttttccca gaaaaaggga
     2101 cgggtaaaaa gttgaaagat gcctttccaa gcagcaatac tggtaagcca ccaatacaaa
     2161 ggatttaaga gcgaataaaa aatgagcctg tctattcctc gtctgaaaac tgccaacatg
     2221 ctcagataaa tccctaaaaa gttaccaacg atgaggttaa aaacccccaa gtataagata
     2281 gggcctgaaa aatagctttg caaaaattcg ccccgattga aaagccaaat aaaaaagaga
     2341 atccataaaa taagggaaaa tgcctgtcca agcgtggttc caccgataaa tacctggaga
     2401 gtaaaccatc ctttccaacc ggaattcctg acgacctgaa aagggtggcg attatgaact
     2461 aaaaaagttt gcagatatcc tttcaaccat cgcgaacgtt gacgaatcca attttttacc
     2521 cggctattgg cttcttcaaa agtggtagag ggaagaatac cgacccgata gcctcgatag
     2581 ctggctctca ttcctaagtc agcatcttca gtgacattat acggatccca tcctcctaaa
     2641 tctcgcagaa tcttggtacg gaaatgattg gatgttccgc caagaggtat gggtaaatgg
     2701 ttcaaataaa gaccaggaag gaggtaatca aaccagtagg aatattccag agtgaataaa
     2761 cgggtgagga tattctgttc ggcattataa aaattaagag cgttttgaaa acacataata
     2821 tccggtggtg atgaacgaaa acaagccacc gcttttttga gctgatctct ttcggggata
     2881 tcttcggcgt cataaatggt gagaaattca cctcgagcaa aagccaggcc aaaattacaa
     2941 gctcgcggtt tggtcttagg cattccttcg ggaacaataa caaagcgaac attataaggc
     3001 ggtttttcag ctttgcagtg aatcaaagtg tcttgatcgt cttcttcgat aaggaatata
     3061 acgtcgagct tttccggagg ataatcaagg ttttgaatag cagttgtcag ttgacgaatc
     3121 acgtttggtg gttcgtggta aagaggcaat aaaatgctgt atgtggggag atcgttggga
     3181 cgaagctgag gctcattttt tttaaataat gagctatttg tgtcgagttt tgctccggaa
     3241 gagcttagta caaaccgaaa tattacactg attgcataaa aaagattagc taaacccaat
     3301 aaaataagga tagttaagcg agggagagta aaaacaccaa ttaagatgag agcacataaa
     3361 gcgcctaaac tgatccattg aacaggaata aaaactctca ttgctgattc ctctggagaa
     3421 cgataaaaaa gaccagaggt agcttcctgt aagaat
//
LOCUS       DAOSOL010000152         3439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_27561_length_3439_cov_3.664894, whole genome shotgun sequence.
ACCESSION   DAOSOL010000152 DAOSOL010000000
VERSION     DAOSOL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27561_length_3439_cov_3.664894"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PK171_04450"
     CDS             complement(<1..997)
                     /locus_tag="PK171_04450"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05474.1"
                     /translation="MNISHFLQKITQQIRSSNRCSLSTIQPVRPFIARLLSRTNYSPI
                     FYITASEEIKRKVFRQIESLSFLNQDGMMAIGVDFEEKYSGIVLQKTLREKKNLVIVL
                     NVNELDQEVVELENFMSLSVLLEKGKEYQRDQLIDQFYRMGYERKDFVREKGDIAIRG
                     EVVDVYPPDGIEPIRTYWLGNLLEKMKYFQADTQRTDHDLEHALVIPSDGEFKTVPLI
                     KLIMEQAHLIFWDDVFFENINLSHIPQVIAGIVSKYDLPEIVISAARPPSYFGEISKL
                     ISGLKENNRGETYLIFPSEKVELLAAILDEEKIPYSREVNSSNQIFLLKGFPVEGF"
     gene            complement(994..1572)
                     /locus_tag="PK171_04455"
     CDS             complement(994..1572)
                     /locus_tag="PK171_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05475.1"
                     /translation="METKKIAKGISIICQPTFIPIALYFIVSLSVVPTVTGIWYALLG
                     IVFIALAPIALVFILARCNKISDPDLPDRRERFIPYLSIVGLYLIGFIVFWYLGAPYQ
                     ILAITASYIAVTFFGAFISLFWKISMHMAGVAGPVTALVFLVNPLFVWAYILLIPIGW
                     ARYILKKHTFPQIVIGSGFSILLTYLVIRFFS"
     gene            complement(1594..2163)
                     /gene="pth"
                     /locus_tag="PK171_04460"
     CDS             complement(1594..2163)
                     /gene="pth"
                     /locus_tag="PK171_04460"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOT05476.1"
                     /translation="MFLVVGLGNPGQKYQYSRHNVGFRIVDNLSEQRQWRWNESKRFQ
                     WCHGKIDSRELYLVKPLTYMNLSGLGLLSFLSFIAKSFEDKIIIHDELDLPPGRIRIS
                     RGGGTAGHKGVLSIAEYCNLEKFIRLRVGIGKPIQKDEITEYVLLKPPADEAAVLADS
                     EKRSVEALICIVTHGVHYAMNRFNSISAS"
     gene            complement(2164..2832)
                     /locus_tag="PK171_04465"
     CDS             complement(2164..2832)
                     /locus_tag="PK171_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOT05477.1"
                     /translation="MNQREILTVNTEKRESIGKENMKRLRRSGWVPGIYYAQESKEGN
                     TIMLKVQESELKRVLKIPGATHQLLNISIDGNIHRGIIKNLQRNPIKEQVLHIDFYGV
                     RADQKITLKVPLMFKGEAPGVKAGGTLEQVMQEVEIECLPDVIPEFIEVDINGLEIGD
                     AVHLKNVKVPEEIELTENLEDVVVVVTPPVVLEEEVPEEEIETPEPEVVKKGEKKEKE
                     AEED"
     gene            complement(2913..>3439)
                     /gene="prs"
                     /locus_tag="PK171_04470"
     CDS             complement(2913..>3439)
                     /gene="prs"
                     /locus_tag="PK171_04470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOT05478.1"
                     /translation="VDNLVAQSLLARYFASKNLSDVVVVSPDVGGTARARNFANHLGV
                     DLAVIDKYRPTYAEVQVMDVIGNVRGKRAILVDDLIDTAGTLVEGAQALKDRGVIEVY
                     ACATHPVFSEPALDRIKNSCLKEVVVTNTIPSNSEELSIRTNDKVRVLSVAPIFAEAI
                     KRIYSELSVSELFS"
BASE COUNT         1059 a          736 c          603 g         1041 t
ORIGIN      
        1 taaagccttc gactggaaaa ccctttaaaa gaaaaatttg gtttgagcta ttgacttctc
       61 ttgaataagg gattttttct tcatctaaaa tcgctgctaa taactcaact ttctctgatg
      121 gaaaaattaa ataggtttcc cccctattat tttcctttaa tccactgatt agcttagata
      181 tctcgccaaa ataagaaggt ggacgagcag cggaaatgac tatctcaggg aggtcatatt
      241 tcgatacaat cccagcaatc acctgaggga tatgggatag attaatattt tcaaaaaata
      301 catcatccca aaaaatgaga tgagcttgtt ccattataag tttaatcaag ggtacggttt
      361 taaattctcc atcactggga atgactaagg cgtgttccaa atcatggtca gtcctttggg
      421 tatcggcttg aaaatatttc attttttcta ataagtttcc caaccagtaa gttcttattg
      481 gttcaatacc atcgggggga tagacatcaa ccacttcgcc acgaatggct atatcaccct
      541 tctctctaac aaaatccttt ctttcatatc ccattcgata gaattgatca attaattgat
      601 cgcgctgata ttcttttccc ttctccaata atactgacag actcataaag ttttctaact
      661 caacgacttc ttggtcgagt tcattaacat ttaagacaat aactaaattc tttttttcac
      721 gtagggtttt ttggagaaca attcctgagt atttctcctc aaagtcaaca ccaatagcca
      781 tcatcccatc ttggtttaaa aaagaaagac tttcaatttg ccgaaaaact tttcttttta
      841 tttcttcact ggcagttata taaaaaattg gagaataatt ggttctggat aaaagtcggg
      901 caataaaagg tcggaccggt tgaattgttg aaagtgagca ccggtttgaa gatcggattt
      961 gttgggtaat tttttgaaga aaatgggaaa tattcatgaa aaaaaccgaa tgactaaata
     1021 ggttaacaag atcgaaaatc cactcccaat tactatttgt ggaaaagtat gctttttaag
     1081 aatatagcgt gcccatccaa tcggtataag caaaatatag gcccatacaa aaagggggtt
     1141 cactaaaaat accaaagcgg taactggtcc agcgacaccg gccatatgca tgctgatttt
     1201 ccaaaaaagc gatatgaaag ctccaaaaaa agtaacggca atataagaag ccgtaattgc
     1261 caatatttga taaggagcac cgaggtacca aaaaacaata aaaccgatta ggtataaccc
     1321 tacaatactt aagtaaggga taaagcgctc gcgtcgatcg ggaagatcag gatcggatat
     1381 cttgttacaa cgggcaagga taaaaactaa agctattggt gctagagcaa taaatacgat
     1441 acccagcaaa gcataccaaa ttcccgtaac cgtgggaaca acagataacg aaacgataaa
     1501 ataaagagct attggtataa aagtgggttg acaaataatg gatattcctt tggctatttt
     1561 tttggtttcc acaacgatgt atctcctcgg cagttatgac gcagaaatgg agttaaaccg
     1621 gttcatggca taatgaacgc catgagtaac aatacagatt aaggcttcga ctgatcgctt
     1681 ttctgaatca gcgagaacag ctgcttcatc ggcaggaggt ttgagcaata catactcagt
     1741 aatttcgtct ttttgtattg gttttccaat gcctacccgt aaacggataa atttttctag
     1801 attacagtat tcagcaatgg aaagaacgcc cttatggccc gccgtaccgc caccgcgact
     1861 gatccggatt cttcctggag gtagatcaag ttcatcatga ataatgatct tatcctcaaa
     1921 agatttggct ataaaactca aaaaggataa aagccccaaa ccactcaggt tcatataagt
     1981 aaggggcttt accagataga gctcacggct gtcaatttta ccgtgacacc attgaaagcg
     2041 ttttgattca ttccatctcc attgacgctg ttcagacaaa ttatcaacaa ttcgaaaacc
     2101 aacattatga cggctgtatt gatatttttg accgggattt ccaagtccga caaccaaaaa
     2161 cacttagtct tcctctgctt ctttctcctt cttctcacct tttttaacca cttctggttc
     2221 gggtgtttca atttcttctt ctggaacctc ttcctctaaa actacgggag gtgtgacaac
     2281 cacaacaacg tcttcaaggt tttcagtaag ttcaatttct tctggaactt tgacattctt
     2341 aaggtgaact gcgtcaccga tttccaggcc gttgatatca acttcaataa attcgggaat
     2401 gacgtcaggc aaacattcaa tttcaacttc ttgcataacc tgttccagag taccacctgc
     2461 cttaactccc ggtgcttcac ccttgaacat aagtggtact tttaaagtaa tcttctgatc
     2521 ggctcgtacc ccataaaaat caatatgcaa aacctgttct ttaatgggat tcctctgaag
     2581 attcttgata atccctcgat gaatattacc atcgatagaa atatttaata actggtgggt
     2641 tgctcccggt atttttaaga ctctttttaa ctcactttct tgaactttta acataatcgt
     2701 attgccttct ttactttctt gagcataata aatcccgggc acccaacccg accttcttag
     2761 ccttttcata ttttcctttc caatcgactc tctcttttcg gtattaacgg tcaagatttc
     2821 tctttgattc atagcattcc ctccgctctc aagcgtatga tagtttagat ttgactgtcc
     2881 ggttatttat actttaaacc gacggagttt tctcacgaga aaagctcact gacggatagt
     2941 tcactataaa ttctttttat tgcctcagca aaaattggtg ccactgatag aactctcacc
     3001 ttatcattgg ttcggatact caactcttct gaattggaag ggatagtatt ggttacaacc
     3061 acttctttca aacaagaatt cttgatacga tctaaagctg gttcactaaa caccggatgg
     3121 gtggcacaag catacacttc aataactcct cgatctttta aagcttgcgc tccttcaact
     3181 aaagttccag cagtatcaat aaggtcgtca actaatatcg ctcttttccc ccgaacattt
     3241 ccgattacat ccatgacttg aacctcagca taggtcggtc ggtatttatc aatgacagct
     3301 aaatcaacac ccaaatgatt agcaaaattt cgagctcgtg ctgttcctcc cacatcgggg
     3361 cttaccacta caacatccga taggtttttc gaagcaaagt aacgagccag gagtgattga
     3421 gcaactaaat tgtcgacgg
//
LOCUS       DAOSOL010000153         3362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_28360_length_3362_cov_3.933777, whole genome shotgun sequence.
ACCESSION   DAOSOL010000153 DAOSOL010000000
VERSION     DAOSOL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28360_length_3362_cov_3.933777"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PK171_04475"
     CDS             <1..696
                     /locus_tag="PK171_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05479.1"
                     /translation="GIIGLALAIIRKEDIIMIFTGIPKMKLLRGQNNIAKILDTSSIV
                     DGRIADICKTGFVEGDMIIPRFVLKELQQVADSQDPLKRSRGRRGLDILNKMRKEKKV
                     NIRIVERDFVEIRDVDAKLIKMAKVMGAKVITNDYNLNKIAELEGVEVLNINELANSL
                     KPYVLPGEELRVQIIREGKESDQGVGYLDDGTMVVVEGGKKYINVVIDTIVTSVLQTP
                     AGRMIFAKPRGKM"
     gene            738..1430
                     /gene="ispD"
                     /locus_tag="PK171_04480"
     CDS             738..1430
                     /gene="ispD"
                     /locus_tag="PK171_04480"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOT05480.1"
                     /translation="MFIALIVAAGTGERMNSIIPKQFLPVLGKPVLAYTIDAFEKSPL
                     IDEIIVVINENHEKQFQEQILNHIQTKKVLHYVFGGGTRQDSVYAGLELIFNRPDDYV
                     LIHDGVRPLVSEEILMRSVEGVMKYGAVCCAIPSVDTLKISKNNNYIDSTLDRSVVWR
                     AQTPQSFKISIIWEAIQKAKVDGFYATDDSAMVERIGIPVYLVLGSEENYKITTPQDF
                     MRAEENLRWIKS"
     gene            1415..1912
                     /gene="ispF"
                     /locus_tag="PK171_04485"
     CDS             1415..1912
                     /gene="ispF"
                     /locus_tag="PK171_04485"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOT05481.1"
                     /translation="MDKILTGFRVGLGYDIHPFVEGKKLVLGGVTVPYHLGLSGHSDG
                     DVVCHALMDSLLGAASLPDIGYWFPNQDERFLNAYSLLLLEEITQKIRSEGLDIINFD
                     CTLIAEFPRVMPYVMEMKQNLSRVTGVDIQSIGLKATTNERLGSLGKGEGIACLSICL
                     IWKNV"
     gene            1876..2628
                     /locus_tag="PK171_04490"
     CDS             1876..2628
                     /locus_tag="PK171_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOT05482.1"
                     /translation="MPIHMSYLEKCIKGMSVTLILSLVVFIFSNLTYANKSIPDRLVN
                     RNSGTIFISGRRVMRIRHSGDYSSIDERTEKIYKRLSDLFTHPPSGAINFYVAPESKQ
                     VIAYCNQKLLFSVYPSDASLNNSEMLDLALLWLNQIQMEFYRLYDGYEGRVVNKIIGL
                     ASWYGPRFSGRTTACEEVFNPYAFTAAHRTLPIGSILLVSNPDNGKKVVVRVNDRGPR
                     SKNREIDLSLAAAKAIGIHQSGVDDVTIEVIE"
     gene            2906..>3362
                     /locus_tag="PK171_04495"
     CDS             2906..>3362
                     /locus_tag="PK171_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOT05483.1"
                     /translation="MDIYLYNTLTQKKELFVPQNKGKIGFYACGPTVYDLIHIGNARV
                     FVIFDCLRRFFEQVGYEVNFVQNFTDVDDKIINRAREKGLQPEEIAANYIEQYLIDAE
                     RLGINQPTVSPRATEHIQDIIALIQRLEDQGFTYSLESDILFDVSRFSGY"
BASE COUNT         1032 a          551 c          756 g         1023 t
ORIGIN      
        1 gggattattg gtttggcttt agcaataatt cgcaaagaag atattattat gattttcacc
       61 ggaattccca aaatgaaact tttacggggg caaaacaata tagctaagat attggatacc
      121 agttcgattg tcgatggacg aattgcagat atttgtaaaa ctggttttgt tgaaggtgac
      181 atgatcatcc ctcgatttgt tttaaaagaa ttgcagcagg ttgccgattc tcaagatccc
      241 ttgaaaagaa gtcgaggtcg gcgaggtttg gatattttaa ataaaatgag aaaagaaaaa
      301 aaggttaata ttcgtattgt agagagagat tttgtcgaga tacgggatgt tgatgccaag
      361 ttaatcaaga tggccaaagt aatgggagcc aaagtcataa ccaatgatta taatttgaat
      421 aaaattgctg agttagaagg agttgaagtt cttaatatca atgagctcgc caactcatta
      481 aagccctatg tattgcctgg agaggaactg agagtacaaa tcattcgaga ggggaaagaa
      541 tccgatcaag gagtggggta tcttgacgat ggaaccatgg tagtggttga aggcgggaaa
      601 aaatacatca atgttgtaat tgatactatt gtcaccagcg ttttacaaac tccggcaggg
      661 aggatgattt ttgccaaacc gagaggaaaa atgtaaaata gagaagttca ttatcctgga
      721 ctatgaggtg atattgtttg ttcatagcgc tgatcgttgc tgccggaact ggagaaagaa
      781 tgaactcaat tattcccaag caatttttac cggttttagg gaaaccggtg ttggcatata
      841 ccattgatgc atttgagaaa tcacccttaa tagatgaaat aattgtagtc attaacgaaa
      901 atcatgagaa gcagttccag gaacagattc tcaatcatat tcaaaccaaa aaggttttac
      961 attatgtttt tggtgggggt accaggcaag attcggttta tgctggatta gagttaattt
     1021 ttaaccgccc tgatgattat gttttaatcc atgatggggt tcgacctttg gtgagtgaag
     1081 agatactcat gcgctcagta gaaggggtta tgaaatatgg tgcagtttgc tgcgctattc
     1141 cttcggttga taccttaaaa ataagtaaaa ataacaatta tattgattca actttagatc
     1201 gttcggtggt ttggagagcc cagacccctc aatcttttaa gatttctatc atttgggaag
     1261 ccatccaaaa agccaaggtt gacggttttt atgcgacaga tgattcagcc atggttgagc
     1321 ggatagggat tccggtttac ttggtattgg gttctgaaga gaattataaa ataactaccc
     1381 cccaggattt tatgcgagct gaggagaatt taagatggat aaaatcctga ccggctttag
     1441 ggtgggttta ggatatgata ttcatccttt tgttgaaggg aagaaattag ttttaggtgg
     1501 tgttaccgta ccctatcatc ttggtttatc cggccattcc gatggggatg tggtttgcca
     1561 tgcgttaatg gattctttgc tgggagcagc gtcattacca gatatcggtt attggtttcc
     1621 caatcaagat gagcgtttcc ttaatgctta tagtctttta ttgttggaag aaataactca
     1681 aaaaatccgg agcgaggggt tggatatcat taattttgat tgtaccctga ttgctgaatt
     1741 tccccgggtc atgccttatg ttatggaaat gaaacaaaac ttatcccgag tgactggggt
     1801 agatattcag tcgatcggcc ttaaggcgac gaccaacgaa agacttggaa gtttaggaaa
     1861 gggggaaggc attgcatgcc tatccatatg tcttatttgg aaaaatgtat aaaagggatg
     1921 tcggtaaccc ttattttgtc cctggtggtt tttatttttt ccaatttaac ttatgcaaac
     1981 aaatccatac cggatcgatt ggtaaatcga aattcaggaa caatttttat tagtggaagg
     2041 agagttatgc ggattcgaca ttccggtgat tattcttcga ttgatgaaag aaccgaaaaa
     2101 atttataagc gattgtcgga tttgtttaca catcctccgt cgggtgccat taatttttat
     2161 gtagcacccg aatcaaaaca ggtgatagct tattgtaatc aaaagcttct tttttcggtt
     2221 tacccaagcg atgcctctct aaataactcc gaaatgttag accttgctct gctctggtta
     2281 aatcagatcc aaatggaatt ttatcgttta tatgatggat acgaaggacg agtagtcaat
     2341 aaaattattg gcttagcttc ttggtatggg ccaagattct ccggaagaac aacggcatgc
     2401 gaagaggtat ttaatcctta tgcttttacg gcagctcatc gaaccctccc gattggctcg
     2461 attcttttgg tttcgaatcc tgataatggc aaaaaggttg ttgtaagagt aaacgatcga
     2521 ggaccgagaa gtaaaaatcg agaaatcgac ctatcattgg ctgcagcgaa agccattggg
     2581 atccaccaat ctggtgtcga tgatgtgact attgaggtga ttgagtaggt tttactcggc
     2641 aaagaataaa tttaacttca ttcaaaaacc taagggcatg ataaatcaag cccctaccaa
     2701 agaatataaa atgtaggggc gcaatttatt gcgcccaatt aatgtagcga cctgccatgg
     2761 catgtcgaat cttgggtttc atcttcatct ggtgctacca agtggcatga ttatctataa
     2821 tggaaaaagc caaataatta tattccttat ggcagaatct atgataggta ttattttata
     2881 atgaaaaaca ggaaagggaa cgagattgga tatctacctc tataatacac taacccaaaa
     2941 aaaagaattg tttgttccac agaataaagg aaaaattggt ttttatgcat gcggacccac
     3001 cgtttatgac ttaattcata ttggaaatgc cagagtattt gtaatatttg attgtttgcg
     3061 acggtttttt gaacaggtag gctatgaagt taactttgta caaaatttta ccgatgttga
     3121 tgataaaata atcaatcgag ccagggaaaa gggtcttcaa cccgaagaaa tagcggctaa
     3181 ttatattgaa caatatttga ttgatgccga aagattaggc atcaatcaac caaccgtttc
     3241 cccccgggcg actgagcata ttcaagacat tatcgccctc atccaacgac tcgaagatca
     3301 aggttttact tactctcttg agagtgatat tttgttcgat gtttcacgtt tttcaggtta
     3361 cg
//
LOCUS       DAOSOL010000154         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_28374_length_3361_cov_3.727768, whole genome shotgun sequence.
ACCESSION   DAOSOL010000154 DAOSOL010000000
VERSION     DAOSOL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28374_length_3361_cov_3.727768"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..963)
                     /locus_tag="PK171_04500"
     CDS             complement(256..963)
                     /locus_tag="PK171_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT05484.1"
                     /translation="MAVALGIVRHVEAHQAQKQKLRKQQDNMGGFSYILIFAVLIVGA
                     IVWGLKRKKTVSVSDFWQEKESEYSEKRILSSFARYLGGHARFENTTEGLLFLMSKSL
                     WFENFEKGANIFGFAFPFEKVIFRIPLSRIGQTKTIPEKELISQGILHPNMNVRRVNR
                     RPEYLSVNYIDEWGREKELFFDSMIDLSQWHQAIKAALENYIPDEEEEKIGACPKCGK
                     KVSPDFKLCPYCGCRLS"
     gene            complement(1269..2897)
                     /gene="groL"
                     /locus_tag="PK171_04505"
     CDS             complement(1269..2897)
                     /gene="groL"
                     /locus_tag="PK171_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779552.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT05485.1"
                     /translation="MMAKSIIFEEEARQSILRGVKILSEAVKVTLGPKGRNVVIEKKF
                     GSPTITKDGVTVAKEVELADPKENVGAQLVKEVASKTSDVAGDGTTTATVLAESIYRD
                     GLRNVAAGSNPMLLKRGIDKAVDEIIKKLKEMSREVGERKEIAQVAAIASNNDESIGN
                     IISDAMEKVGKDGVITVEEAKGLETTLEVVEGLQFDRGYLSPYFITDPDRMEVVLENP
                     YILIYEKKISAIKDLLPLLEKVVQRGRPLLIIAEEVEGEALATLVVNKLKGVINGAAV
                     KAPGFGDRRKAMLEDIAIMTSGNFISEDLGIKLENLNLDDLGEATKVVIDKENTTIIE
                     GKGTKEKIVARMNQIKRQIEETTSDYDREKLQERLAKIAGGVAVIHVGAATEAEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALIRCASIIDDLSLVGDEKTGANIVKKALEEPA
                     RMIAENAGEEGSIIVEKIKNSEKLTFGFNALTNEFVDMYEAGIIDPTKVARAALQNAA
                     SVASLMLTTESVVTEIPEKEKTPPMMPPGPDMGY"
     gene            complement(2915..3253)
                     /gene="groES"
                     /locus_tag="PK171_04510"
     CDS             complement(2915..3253)
                     /gene="groES"
                     /locus_tag="PK171_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT05486.1"
                     /translation="MVFDKLINTSERGEYRVNIKPLGDRILVKRIEEEEVHKGGIIIP
                     DTAKEKPQQGSIVAVGSGKVNEDGNRMPLEVKAGDRVLFGKYAGSEVKIGDEEYLIMR
                     EDDILGIIEG"
BASE COUNT          946 a          749 c          512 g         1154 t
ORIGIN      
        1 ctgtacttcc gatataaaca ctcaaatctg tataatgccc aattttcagc cctttcatga
       61 attaaccttt aaatcctttc tcattgattt attaatgaat tgtatcgtta tataataaaa
      121 gaaaatagtg tttgaatgat aataacttta tttttaataa taacctatat atccaaacta
      181 tttacttttt tattttttat gagaaaaaac gagtgaaatg aggatggaca aaacgtcaat
      241 atttaaaaga catttctatg acaagcggca accacaatat gggcagagtt taaaatcagg
      301 agatactttt ttcccgcatt tcgggcatgc accaatcttt tcttcttctt catctggtat
      361 ataattttct aaagcagctt ttatagcttg atgccattgt gaaaggtcaa tcatactatc
      421 aaaaaataat tctttttctc ttccccattc atctatataa ttaactgaaa gatattctgg
      481 tctccgattg actcgtctta cattcatatt gggatgtagt atgccttgag agatgagctc
      541 tttctcggga attgttttag tttggccaat ccgggagagt ggaattcgaa atatgacttt
      601 ttcgaagggg aaagcaaatc caaaaatatt tgcacctttt tcaaaattct caaaccataa
      661 acttttagac attaaaaaaa ggaggccttc cgtcgtattc tcaaaacggg catgacctcc
      721 taaataacga gcaaaagatg aaaggattct tttttccgaa tattcacttt ctttttcttg
      781 ccaaaaatca gagactgaga ccgtcttttt tctctttaaa ccccagacaa tcgccccaac
      841 tattaaaact gcaaaaatca gaatatacga aaagccgccc atgttatcct gttgttttcg
      901 aagtttttgt ttttgagctt gatgtgcttc cacatgtcga acaattccca gtgctacagc
      961 cacttgaaca gctcgaatga gcagttgaag aatgagaaga acggtaatcg gtacaataaa
     1021 aaccgctccc cttaaaagac aaacctaccc ctttgcttac cagctttcta actctccctt
     1081 tacagatagg gcattgagtt attggtttat cagaaaatga ttgcaattct tcaaacacat
     1141 ttctgcactc ggtacactga tattcataaa ttggcatttt tgctacctcc caacctatca
     1201 actgcctgaa aaattagccc ggagaaatca tccctccggg ctacaatatt atagcatttt
     1261 tttacttttt aataacccat atcaggacct ggcggcatca ttggaggtgt tttttccttt
     1321 tcaggtatct cggtcacgac tgattcggta gtaagcatta aagaagccac actagctgca
     1381 ttttgtaaag ctgcgcgggc tactttggtt ggatcaataa tgcctgcttc atacatatca
     1441 acaaactcat tggttagtgc attgaatcca aaggttaact tttcagaatt cttgattttc
     1501 tcaacaatga ttgaaccttc ttctccggca ttttcagcaa tcattcgagc tggctcttct
     1561 aaagcctttt tgacgatatt agctccggtt ttttcatctc caaccaaaga taaatcatcg
     1621 attattgaag cgcagcgaat tagtgcaacc ccgccaccag gaacaatccc ttcttctact
     1681 gcagcacgag tggcatgaag agcatcttca acccgggctt tcttttcttt catttcagcc
     1741 tcggtcgctg ctccaacatg aataactgca acaccaccgg caatttttgc taatctttct
     1801 tgtaatttct cacgatcata atcagaagtg gtttcttcta tctgacgctt gatctggttc
     1861 attctggcaa ctattttttc tttagtacct tttccttcaa taatggtggt gttttcttta
     1921 tcaattacta ccttggttgc ttctcccaaa tcatcaaggt ttaggttttc taatttaatt
     1981 cctaagtctt cggagataaa attaccacta gtcattatag cgatatcctc taacatggct
     2041 tttctccgat caccaaaccc cggtgcttta actgccgctc cattaatgac accctttagt
     2101 ttattaacca ccaaagtagc aagagcttca ccttcaactt cttcagcgat gataagtaag
     2161 ggccttcctc gttgaacaac cttttctaat agaggaagca agtctttaat agctgagatc
     2221 ttcttctcat aaataaggat ataaggattt tctaaaacta cttccatacg atcggggtca
     2281 gtgataaagt aaggtgacag gtatcctcta tcaaactgaa gaccttcgac aacttctaaa
     2341 gtagtttcta gtcctttggc ttcttcaacg gtgataaccc catcttttcc aactttttcc
     2401 atcgcatcag atataatatt tccgatagat tcatcattat tggaagcaat ggcagcaacc
     2461 tgagcaattt cttttctctc accgacttct ctgctcattt ccttcagttt tttaattatt
     2521 tcatcaactg ctttatcaat accccttttt aataacatcg ggtttgatcc agccgcaaca
     2581 ttcctcaaac cgtcacgata aatactttca gcaagaacag tagctgttgt tgttccatct
     2641 ccagcaacat cactggtttt tgatgcaact tcttttacta gctgagctcc aacgttttct
     2701 ttgggatcag ctaattcaac ttctttagca acggtaactc catctttggt aatagttggt
     2761 gatccaaatt tcttttcaat aacaacattt cttccttttg gacctaaagt tactttgact
     2821 gcttcagaca gaattttgac tcctcttaaa attgattgtc tggcttcttc ttcgaaaatg
     2881 attgatttcg ccattatcgt gtacctccct tccgctaccc ttcgataatt ccgaggatgt
     2941 catcctctcg cataatgaga tactcttcgt cgccaatctt tacttctgaa ccagcatact
     3001 tcccgaaaag tactcgatca cctgctttca cttcaagagg catacgattt ccatcttcat
     3061 ttacttttcc actaccaaca gcaacaattg atccttgctg aggtttttct ttcgcggtat
     3121 cgggaataat aataccgcct ttatgaactt cttcttcttc gatccttttt actaggatgc
     3181 gatctccgag tggtttaata ttcactcgat attcacccct ttcggaagta tttattaatt
     3241 tatcgaatac cattttaccc attttttttg aaattgcaag agggttaaag tcaatcaata
     3301 tcaatattgt gtcattatga caagttttca ctttgataat tggttattat tacattaccc
     3361 t
//
LOCUS       DAOSOL010000155         3322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_28793_length_3322_cov_3.836547, whole genome shotgun sequence.
ACCESSION   DAOSOL010000155 DAOSOL010000000
VERSION     DAOSOL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28793_length_3322_cov_3.836547"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1388
                     /gene="tyrS"
                     /locus_tag="PK171_04515"
     CDS             156..1388
                     /gene="tyrS"
                     /locus_tag="PK171_04515"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT05487.1"
                     /translation="MSFEKRATEDLEILCRGVEDIISREDLQKKLNRYYQTGQPLIVK
                     EGFDPSAPDIHLGHTVTLRKLRQFQALGHQVVFLVGDFTGRIGDPTGKKETRKQLTEE
                     EVLVNAKTYSDQAFKILDPEKTIIEFNSKWLKNLSFVDIIEITAHFTVARMLEREDFH
                     QRISNGKPVGLHEFLYPIMQAYDSVSLRADVELGGTDQRFNLLMGRDLQREFGQEPQV
                     VMMMPLLEGTDGVEKMSKSLGNYIAVNDPPFDMFGKIMSIPDSLIKKYFILLTDIPMN
                     TILQWEKDFTNDLLHPRDWKIILSKEIVSIYHGSDRANQALEDFERAFAKKESPEDAQ
                     ILTLTNDDLKDEKIWIISLLLKTGVSHSKSELRRLVEQGGVYLDNMRIADPNSDIQIK
                     DDQFLRVGKKHFFRIKKK"
     gene            2035..3090
                     /gene="nusA"
                     /locus_tag="PK171_04520"
     CDS             2035..3090
                     /gene="nusA"
                     /locus_tag="PK171_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT05488.1"
                     /translation="MNKDLFFAIEQIERAKGISRETLKQAIEAALLSAYKKNFKGPHK
                     AVEVILDPNTGEVKVYTHKLVNPENGETLDIDEYEVLSNQSPNRVGPGEIVQVEITPK
                     DFGRIAAQTAKQVIMQRIREAERSLVYDEFLERAEDIVTGVINRKEGKNIIIDLEKIE
                     SLLPPNEQIPSENYRIGARMKFYIIEVKKTSRGPRIVLSRSHPGLVKRLFELEVPEVH
                     DGFVTIKAIAREPGARTKIAVESRDAKVDPVGSCIGNKGARVKHITDELRGEKIDIIR
                     WSNDPKVFISDALSPAHVTLVELDSDSNTARVYVPDDQLSLAIGKEGQNARLSARLTG
                     WRIDIKSDQEIKEVPSH"
     gene            3090..>3322
                     /locus_tag="PK171_04525"
     CDS             3090..>3322
                     /locus_tag="PK171_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOT05489.1"
                     /translation="MKKKKIPLRLCLSCRLRKEKKSLVRIVASPTGEIDIDMTGKKPG
                     RGAYICPNTECNARINRQLLSFALKTEVIQEDV"
BASE COUNT         1101 a          598 c          677 g          946 t
ORIGIN      
        1 tttgttgttt cattatatct cagtaaactt tttcaagttc cctattttta ttcggttttt
       61 tatattatga taggagaatg tattgcctgt tatggaatcg gatatccaat cttaaaaatc
      121 ttgcttaaac gtggaatagt aaggagatga tcagattgag ttttgaaaaa agagcaactg
      181 aagacctcga aattctctgt cgaggggttg aagatataat tagtcgcgaa gacctccaaa
      241 aaaaattaaa tcgctattat caaactggac agcccttaat tgttaaagaa ggatttgacc
      301 ccagtgcacc cgatatccat cttgggcata ccgttaccct cagaaaacta agacagtttc
      361 aagctctcgg tcatcaagtg gtatttctgg taggtgattt tactggacgc atcggtgatc
      421 caaccggtaa aaaagaaacc cgcaagcaat tgaccgaaga agaagtctta gtcaatgcga
      481 aaacctattc agatcaagct tttaaaattc tcgatcccga gaaaaccatc attgagttta
      541 atagtaaatg gcttaagaat ctttcctttg ttgatataat tgaaattact gctcatttta
      601 ccgtagcccg tatgctggaa agggaagatt ttcatcaacg tatatcaaat ggaaaaccag
      661 tcggattgca tgaatttctt tacccaatta tgcaggctta cgactcggtt tctctacgtg
      721 ccgacgtaga attaggaggg actgaccaac gttttaactt actcatgggt cgtgatttac
      781 aaagggagtt tggtcaagaa ccccaagtag tcatgatgat gccattgtta gagggtactg
      841 atggagttga aaaaatgagc aaaagtctcg gcaactatat cgcagttaac gatccaccct
      901 ttgatatgtt tgggaaaatt atgtcaattc ctgattctct aataaaaaag tattttatac
      961 tcctcaccga tatccccatg aatactattc tacaatggga aaaagatttt accaatgatc
     1021 tcctccatcc tcgagattgg aaaataatcc tgtctaaaga aatcgtctct atatatcatg
     1081 gatcagatcg tgccaaccag gcattagaag acttcgaacg agcatttgcc aaaaaggaaa
     1141 gtcccgaaga tgctcaaata cttactctta ctaatgatga ccttaaagat gaaaaaatct
     1201 ggattatttc tttactcctt aaaactggtg tatctcattc aaaaagtgaa ttacgaagat
     1261 tggtcgagca gggaggagta tatcttgata atatgaggat agctgatcca aattctgata
     1321 tccaaattaa agatgaccaa tttcttcggg tagggaaaaa gcactttttc agaatcaaga
     1381 aaaaataaga atgagaagcc aattattttt aaagaatacc tacctatgct cattcttaac
     1441 ctggaaatat agattaaaat attttattag tcctttatta tgcggaatgg agcgacgtag
     1501 caatcgcctc taataataat atattgaagg aaaaatttaa aagatgtggc cgttaccagc
     1561 tcaaaagatg agggctcaag acaacaatac aacttaaaaa gtatactctc ccccgagcta
     1621 gacagtcccc ttcccaagga gatgaattat tgaattcatt cctccaggaa agaagaaata
     1681 tgagggggtt gtgttgtgga catttttttc tcatcactgc ttttaaattc ctgactcgat
     1741 atgagccaca cgtcaatata tttatagagg ataaatttgc aacccatgct cagttagtat
     1801 aaaatagtag ggatagaaca cctagttact taattttgcc gaataaggtt ggtgttcttt
     1861 gaatgattag ttttattatt tgaaccttac atcgaagact gatcctgttt tataggagaa
     1921 tgaaggcata caaacaagaa agagtgggtt tgaagcccac tctttcttgt ttgtatgaaa
     1981 gaccaatatt ttttattatc gaacaagaaa gtaatagtat gcagggggtt taatatgaat
     2041 aaagatcttt tttttgcaat cgagcaaatt gaaagagcaa aggggatttc gcgtgagacc
     2101 ctaaaacaag ctatagaagc ggctcttctt tctgcataca agaaaaattt taagggcccc
     2161 cataaagcag tggaagttat tcttgatcca aatactggag aagttaaagt atacacccac
     2221 aaattagtca accctgaaaa tggtgaaacg cttgatatcg acgaatatga agttctatcc
     2281 aaccaaagcc caaatcgagt tggaccggga gaaatagttc aggttgaaat tacacccaaa
     2341 gattttggga ggattgcagc ccaaactgcc aaacaggtaa ttatgcaacg cattcgagaa
     2401 gctgagagaa gtttggtata tgatgaattt ttagaaaggg cagaggacat cgttactggc
     2461 gtcattaacc gtaaagaagg gaaaaatatc atcatcgatc tggaaaaaat tgaatctctt
     2521 cttccgccta atgaacagat accatcggaa aattatcgga ttggagcccg gatgaaattt
     2581 tatataattg aagtgaaaaa aaccagccgg ggtccccgta ttgttctctc tcgatcccat
     2641 ccaggcttag taaaaagatt atttgaattg gaagttccgg aagttcatga tggttttgtc
     2701 accatcaaag ctatcgcccg ggaaccggga gcccgaacta aaattgctgt cgaaagccgg
     2761 gatgccaagg tcgatccggt tggttcttgc attggaaata aaggagcaag ggttaaacat
     2821 ataaccgatg aactccgggg tgaaaaaatt gatattatcc gttggagcaa cgatccaaag
     2881 gtatttattt cagatgcctt gagtccggct catgtcacct tggtcgaact tgattctgat
     2941 tctaatacag cccgggttta tgttcccgat gatcaacttt cactcgccat tgggaaagaa
     3001 gggcagaatg ctagactttc tgcccgtttg accggttgga gaatcgatat aaaaagtgat
     3061 caggaaataa aagaggtgcc aagtcattga tgaaaaaaaa gaaaataccc ttacggcttt
     3121 gcctgtcttg tcgccttcga aaagagaaaa aatcattggt tcggatcgtt gcgtcaccta
     3181 ctggtgagat agatatcgat atgactggaa aaaaacctgg aagaggagcc tatatatgcc
     3241 cgaatacaga atgcaatgct cgtataaata ggcaactcct ttcttttgcc cttaaaactg
     3301 aggtaattca ggaagatgtt at
//
LOCUS       DAOSOL010000156         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_29122_length_3295_cov_3.426235, whole genome shotgun sequence.
ACCESSION   DAOSOL010000156 DAOSOL010000000
VERSION     DAOSOL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29122_length_3295_cov_3.426235"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="PK171_04530"
     CDS             <1..1377
                     /locus_tag="PK171_04530"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT05490.1"
                     /translation="KPGSLHLANSGYIIMYFRDLIMNPGVWEGLKRALKNHEIKIEDP
                     WEQFGLVAPQGLTPEPHPFNAKVILVGDDMVYRLLTLYDEDFKDLFKVKADFDFQLDK
                     NNDVIHSFACYVKKICENENLLPFENSGVARLLEHASRRVSHQDKLVSQFGYLKDIVV
                     ESDHWARQDHSEKVTRSHVERTLKERKNRHNQIEDKIQELIDQGIVLIDIENEVIGQI
                     NGLSVYELGDYSFGKPSRITVKTYLGRQGLINIERESRMSGKIFDKGILIISGYLGHT
                     FAQDKPLSISASICFEQSYEGVEGDSASLAETCAILSSLGEIPIRQNIAVTGSINQKG
                     EVQAIGGVNQKIEGFFQLCKNRGLTSKQGVIIPASNIQNLMLDDEIIQAVREGLFHVF
                     AVKNVDEAIEILTGMKAGKLLDDGTYEPGTIKAQVDEKIRIANEKLKKISEMKENEKK
                     DVSNAADQ"
     gene            complement(1568..>3295)
                     /locus_tag="PK171_04535"
     CDS             complement(1568..>3295)
                     /locus_tag="PK171_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05491.1"
                     /translation="TKTDWIPQTIDDFLKSIKETITLILPPQPWSDHMLQSYFTLYAF
                     LHKTHREQPFRIQSIIAPSQLSSADSKQSYQIYLLENSVSDYQLYGKKLFLTAEGVKT
                     ISAELHRFMIGTSGKTFYSTEEKKSFQPQKITFSHLGYSSFKLEGFGNMEKNLRFYYS
                     DLSKYPTSLTLRLFFNHTPLPSDFKGEALIKLSINSQPVYSQRLDAKSGGQLFPIDLY
                     LSTPLLKPVNTLTFGLSYYPDEEDYRLGMMPFEGFFSEDSYFQVKSGNYAAILSSWKP
                     ITAVPGNKIKMVLPSELDENTLHIAAEILSSIQLYNSRLIPVEIIFGGTHPNNLLSPS
                     SIAIGWNPNYYPEMWNYHWTNFIQIPKLIEHTLSSSEYLNLNLLQKIPAAIYNITIPF
                     GQFVHQFFFSFLSYPIPVQTNRSENHLLIIVSPEIIPNYLSSTVFFESGQMVLKDSIQ
                     SQANLTTSLNEPISCLVFFYQNHIPVILFTTSGQKSIAENYFKKHFNPKTTFLGISGN
                     LVIFEKNGIIDATFPENSPTNDPDKSQGFFSQFTSNQRILFLIILGIIIALVIIFYYQ
                     KNHLTSSDN"
BASE COUNT         1118 a          467 c          712 g          998 t
ORIGIN      
        1 aagcccggat ctctccattt agcaaacagt ggatacatca tcatgtattt ccgcgatttg
       61 attatgaatc ctggtgtgtg ggaaggcttg aaaagagcgt taaaaaacca tgaaattaaa
      121 atcgaggatc cttgggaaca gtttggcctg gttgcaccgc aaggattaac tcctgaacct
      181 caccctttta atgcaaaagt aattctggtt ggagatgata tggtttaccg attacttacc
      241 ctttatgacg aagattttaa agatttgttt aaagtgaagg ctgattttga ttttcagtta
      301 gataaaaata atgatgtcat tcattctttt gcttgttatg taaaaaaaat atgtgagaac
      361 gaaaatcttc ttccttttga aaattcagga gttgcccgtc ttttagaaca tgcttcccga
      421 agagtttctc atcaagataa gctagtatcc cagtttggtt atctcaaaga tatagtggtt
      481 gaatctgatc attgggcaag acaagatcat tctgaaaaag taactcggag tcatgtggaa
      541 cgaaccttaa aggaaagaaa aaaccgccac aatcaaatcg aggataaaat tcaagaattg
      601 attgatcagg gaatagtttt aatcgatatt gaaaatgaag ttattggtca gatcaatgga
      661 ctgtcagttt atgaattagg tgattatagt tttggaaagc cttcaagaat aaccgtgaag
      721 acttatttag gccggcaagg tttaataaat attgaacgtg aatctcggat gagtgggaaa
      781 attttcgata aaggaatatt aattataagt gggtatttag gtcatacatt cgctcaagat
      841 aagccacttt cgatatcagc cagtatctgt tttgaacagt cctatgaagg agttgaagga
      901 gacagtgctt cgttagcaga aacctgtgcc attctttcaa gtctcggtga gattccaatt
      961 cgacaaaata ttgctgtaac tggatctatt aatcaaaaag gggaagtaca agccattgga
     1021 ggagtaaacc aaaaaattga agggtttttc caattatgta aaaatcgcgg attgacttca
     1081 aagcagggag tgattattcc tgccagtaat attcaaaatc tgatgttaga tgatgaaatt
     1141 attcaagcag ttcgggaagg tcttttccat gtttttgcag taaaaaatgt tgatgaagct
     1201 atagaaattc tcactggaat gaaggcggga aaacttcttg atgatggaac ctatgaaccc
     1261 ggaacaatta aagctcaggt tgatgaaaaa ataaggatag ccaatgagaa attgaaaaaa
     1321 atttctgaga tgaaagaaaa cgagaaaaaa gatgtatcaa atgcagctga tcaataattt
     1381 acccgctaag cgacttaaaa ttaggatatt tatctaccaa gacgagagaa agatcataat
     1441 cagttaattt ttcttctggt ttcaaagaat ttaggtattg gccgattaag ctttgcattg
     1501 gaaaaatgaa atgaaaaggt tttgctctct caccaggcaa agcaaaggaa aaagattcat
     1561 tttctcttca attgtcactg cttgttaaat ggtttttttg ataataaaaa attatcacta
     1621 aggcaataat aataccgaga ataatgagaa aaagaattct ttgattagag gtaaattgag
     1681 aaaaaaatcc ctgagattta tcaggatcat tggttggaga attttccgga aaggttgcat
     1741 caataattcc atttttttca aaaatgacta aatttcccga gatgcccaaa aaggtggttt
     1801 tggggttgaa atgtttctta aaatagttct cagcaatcga tttttggcca gatgttgtaa
     1861 aaagaatcac tggaatgtga ttctgataaa aaaataccaa acaacttatt ggttcattca
     1921 ggctggtggt aaggtttgct tggctttgga ttgaatcttt caaaaccatt tggccggatt
     1981 caaagaaaac cgttgaagaa agataattag gaattatttc cgggcttacg attataagaa
     2041 gatggttttc actccgattg gtctgaactg gtatggggta tgataagaaa gaaaagaaaa
     2101 attgatggac aaattgacca aagggaatag taatattata aatggcagca ggtatttttt
     2161 ggagaagatt aagattgagg tattctgaag atgataaagt gtgttcgatc agctttggta
     2221 tttgtatgaa gtttgtccaa tgataattcc acatttctgg gtaataattc ggattccaac
     2281 cgatagcgat tgaagatggg ctaaggagat tattaggatg ggttccccca aaaatgattt
     2341 ccactgggat cagccttgaa ttataaagct gaattgagga gagaatttct gcagcaatgt
     2401 gcaaggtatt ttcatccaac tcagaaggaa gtaccatttt aattttgttg ccgggtacag
     2461 cagtaatagg tttccagctg gacaaaatgg cagcataatt accgcttttt acctggaaat
     2521 acgagtcttc agaaaaaaag ccctcgaaag gcatcattcc cagtcggtaa tcctcttcat
     2581 cgggataata gcttaatccg aaggttaagg tatttactgg tttaagaagt ggagttgata
     2641 gatagaggtc aatgggaaat aactgaccac cgctcttggc atctaaacgt tgagagtaaa
     2701 ccggctgaga gtttatcgac aacttgatca gagcttctcc tttaaaatct gaaggaagtg
     2761 gagtatggtt aaaaaaaagc cgaagagtca atgaagttgg atattttgaa aggtcagaat
     2821 aataaaaacg aaggtttttc tccatattgc caaatccctc taacttgaaa gaagaatatc
     2881 ctaaatggga gaaagttatt ttttgaggct ggaaagattt tttttcttcg gtggaataga
     2941 aggtttttcc actggtacca atcatgaacc tgtgtaattc agctgatata gtttttactc
     3001 cttcagcggt taaaaaaagt tttttcccat aaagttgata atcactaaca gagttttcta
     3061 aaagatatat ttggtagctt tgtttagaat ctgcggagga aagttgagaa ggagcaataa
     3121 tagattggat tcgaaaaggt tgttctcgat gagttttatg aagaaaagca tagagggtga
     3181 aataggattg aagcatgtga tccgaccaag gttggggagg aagaattaga gttattgttt
     3241 ctttaatact tttcagaaag tcatcaatag tttggggaat ccaatcggtt ttagt
//
LOCUS       DAOSOL010000157         3295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_29124_length_3295_cov_3.336420, whole genome shotgun sequence.
ACCESSION   DAOSOL010000157 DAOSOL010000000
VERSION     DAOSOL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29124_length_3295_cov_3.336420"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PK171_04540"
     CDS             complement(<1..867)
                     /locus_tag="PK171_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05492.1"
                     /translation="MNSLRKLLTFRSSNTLWVFIALVVVFSLFTPNKIFINVRNISAI
                     GKLFPDLGIITLGVGMLMICGEFDLSVASVLPFCSYVFARLLFIGTSPLISFIAAILV
                     GMALGYLNGFITVKTRMPSFITTLGTLWFWRGILYVWSKMMPMSIRTYVPNNTPFQLA
                     MAGNLTPFFPIQVLWFLIFAVILGIILHYHRFGNWVFATGDNRDAARAMGIKTDWVKI
                     ICYVIVGALCGFCACMQAVRLGSFAATQGVNFEFKAVAACVVGGTSLRGGIGSMLGIV
                     LGALIIPIIDNGL"
     gene            complement(950..1933)
                     /locus_tag="PK171_04545"
     CDS             complement(950..1933)
                     /locus_tag="PK171_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05493.1"
                     /translation="MKNWLILLIITVLMVSMSSLAFGQEKPAEGMYFRLVTHGGDDPF
                     WAVVQQGMLDAAKELGCQVDIDLAGSDLALQQKRLAEAVAQKPNGIALVVNDDTAWDK
                     PVEEALAAGIPVIGINNDDSQGPAGNKRLCYIGQSEERAGYMIGLRLFQEAKEKGIDL
                     SKAHVAMPVEVPGANYGVVRSNGIKRAGEEFGITSFEIIDGGGLEMTTMEQRETSYLL
                     AHPETTFMIGLGGIATDRLTDSLKAAGKEPGEVIAGGFDTTAATLNGLREGYITATID
                     QQQYLQGYYAVYVLYLYNKFGFTPNIDTGGYLVDSIEKIDLIEKLSPMHVR"
     gene            complement(2029..3153)
                     /locus_tag="PK171_04550"
     CDS             complement(2029..3153)
                     /locus_tag="PK171_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05494.1"
                     /translation="MNSRERLRKAINHQEPDRVPLDLGGIVSGITRIAYRNLKNELKF
                     PVSSNEYLIDRIQQLIKPDEQILDWFQIDTRYAYQEVPPEIWSKDSPDSIWVDEWGIK
                     RRFTGLYFDMIGHPLSRVESLSDLEKLSWPDPHQDQASYDLLQSQVEKLHQSEKAVIV
                     NIIGSCFEFGWYLRGFEKIMMDLVLNPNLACAILDIMLEFQIGQFEELLSRTGNLIDV
                     ILCGDDLATQNSPFVSLDLYRRYIKPRQKKLYDFIKTKTNAPIFYHSCGAVSSFIPEL
                     IEIGIQILNPVQVKAQGMDLHKLKKEFGKQIVFWGGIDTQYVLPFGKPSEVRDEVRRR
                     IDELAPGGGYILAAVHNIQADVPPQNIIAMFEEAIHYGRY"
BASE COUNT         1038 a          729 c          579 g          949 t
ORIGIN      
        1 gagaccatta tcaatgattg gaatgatgag ggcccctaaa acaattccta acatacttcc
       61 aattcctccc cggagagaag tccctccgac cacacaggcg gcaactgctt taaattcaaa
      121 attaactccc tgtgttgccg caaaagaacc gagccgaacc gcttgcatgc aagcacagaa
      181 accgcataaa gcacctacga tgacataaca gataatttta acccaatcgg ttttgattcc
      241 cattgctcga gccgcatctc gattatctcc agtagcaaat acccagtttc caaagcgatg
      301 ataatgaaga ataataccca gtatcacagc aaaaattaga aaccacagta cctggatagg
      361 aaaaaaaggg gttaaattac cagccatagc taattgaaaa ggagtgttat tgggaacata
      421 agtccgtata ctcataggca tcatttttga ccaaacatag agaattcctc tccaaaacca
      481 tagagttcct aaggtagtaa taaacgaagg catccgggtt ttcaccgtaa taaatccatt
      541 taaataccct aaagccatgc ctactaaaat agcagcgata aaactgatta aaggactggt
      601 accgataaat agtagacggg caaatacata ggaacaaaaa ggaagtactg acgccaccga
      661 tagatcaaat tctccacaga tcattaacat cccaaccccg agagtaataa tccctagatc
      721 gggaaaaagc tttccaatgg cagaaatatt ccgtacatta ataaaaatct tattgggtgt
      781 aaaaagagaa aaaactacaa ctaaggcgat aaatacccat aaagtatttg atgagcggaa
      841 ggtcaataat tttcgtaagg agttcatctt ccgacctctt ttattatatg gtatggtaga
      901 tcattagaaa attccggctg gttactatag caaccagccg gaaccaaaat tatctaacat
      961 gcatgggaga aagtttctcg atcaaatcga tcttctcaat actatcaact aaatatccac
     1021 cagtatcaat atttggagta aatccgaatt tattgtagag ataaagtaca tatactgcat
     1081 aataaccctg caggtattgc tgctgatcaa tagttgcggt tatgtatcct tcccgcagtc
     1141 cattcagagt tgcagcggtg gtatcaaatc ctccagcaat aacctcgcct ggttcttttc
     1201 cagcagcttt aagagaatct gtcaagcggt cagtagcaat tccacctaaa ccgatcataa
     1261 aagtagtttc tggatgagct aagagatagg aagtttctct ttgttccatg gtggtcattt
     1321 ccaaaccgcc accatcaata atctcaaacg aagtgattcc aaattcttct cccgctcttt
     1381 taataccatt agaacgaacg acaccgtaat tggctcccgg aacttcaacc ggcatagcta
     1441 cgtgagcttt ggagagatca attcccttct cttttgcttc ctggaacaag cgtaacccga
     1501 tcatgtatcc ggctctttct tcactctgtc cgatatagca aagacgctta tttccggcgg
     1561 gaccctgaga atcgtcgtta tttattccaa taactggtat gcctgcagct aaagcttcct
     1621 ctaccggctt atcccaggca gtatcatcat taacaactaa agcaattcca ttcggttttt
     1681 gagcgacagc ttcagcaagt cttttttgct gcagggctaa gtctgaacca gctaaatcaa
     1741 tatcaacctg gcatcctaat tctttggccg catccaacat accctgttga acgaccgccc
     1801 agaaggggtc atctccacca tgggttacta aacgaaagta catgccctcg gctggtttct
     1861 cctgaccaaa agcaagagaa gacatactta ccattaacac cgtaataatt aataatatta
     1921 accaattttt cattttctgc cctcctaata tttttctcag ttttcattta gaatttaatt
     1981 cttgacaaac tactgaatat caccacctcc tatcaggaat tccaggtttt aatacctccc
     2041 ataatgaata gcttcttcaa acatagcgat aatattttgg ggtggaacat cggcctgaat
     2101 attatggact gccgccaata tataaccacc tccaggagct aattcatcga tccggcgtcg
     2161 tacctcatcc cgaacctcgg atggttttcc aaaaggaaga acgtattggg tatcaattcc
     2221 accccagaaa acgatttgtt tcccaaactc cttttttagc ttgtgtaaat ccataccttg
     2281 cgctttcact tgaaccggat ttaggatttg tataccaatt tcaattaatt ctggtataaa
     2341 ggaagatact gcaccacagg aatgataaaa aataggcgca ttggttttgg ttttaataaa
     2401 atcgtagagc tttttctgac gaggttttat ataccggcgg taaagatcaa gagaaacaaa
     2461 gggactgttc tgagtggcta aatcgtctcc acaaaggatt acatctatga ggtttcccgt
     2521 tcggctgagt aactcttcaa attgtccaat ttggaattca agcataatat ccaaaattgc
     2581 acaggcaaga ttaggattga gtaccaaatc catcataatt ttttcaaacc cacgaagata
     2641 ccaaccaaat tcaaaacagg aaccaatgat attgacaatt actgctttct cactttgatg
     2701 taatttttca acctggcttt gcagtaaatc gtaagaggct tgatcttgat gggggtcagg
     2761 ccaagataat ttctctaaat ctgataggga ttctactcga gaaagtggat gtcctatcat
     2821 gtcaaaatag agtccagtaa aacgccgttt tatgccccat tcatcaaccc aaatagaatc
     2881 cgggctatcc ttgctccaaa tttctggtgg aacttcttga taagcatacc tggtatcaat
     2941 ttgaaaccaa tcaagaatct gttcatcggg ttttatcaac tgctgtattc gatcaatcaa
     3001 atattcattt gaagaaacag ggaatttgag ctcatttttg agattgcgat aagcaatacg
     3061 agtaataccg cttacgatcc ctcctaaatc caaagggacc cgatcgggtt cttgatgatt
     3121 tatcgctttt ctgaggcgtt ctcgactgtt catatctttc ctctccagga ttctattata
     3181 atctattctt ggtttatatt accattattt tctaaacaat gaattgatat tcggtttttt
     3241 ataataaatt ggttgtggaa tttatctttt tttgtattcc acaaccgatg aatta
//
LOCUS       DAOSOL010000158         3279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_29306_length_3279_cov_3.266439, whole genome shotgun sequence.
ACCESSION   DAOSOL010000158 DAOSOL010000000
VERSION     DAOSOL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29306_length_3279_cov_3.266439"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..786
                     /gene="nth"
                     /locus_tag="PK171_04555"
     CDS             145..786
                     /gene="nth"
                     /locus_tag="PK171_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT05495.1"
                     /translation="MIQETAMKIIDILEKRFPDACLLLNYRNAFELLVVTILAAQAPD
                     ERVNQVSPSLFQKYPNPAAMAEADDEEMDKIIQTISFYRQKGRNIRAMSKKIIKDFQG
                     EVPKKVEELVQLNGVGRKTANIVLANGFGIPAIPVDTHVARVSQRLGWSEFSNPDQIE
                     SDLAKIFPREKWIRVTHLLGFLGRYICQAKKPKCWECPVTNWCPYPGKTISKD"
     gene            complement(773..2293)
                     /locus_tag="PK171_04560"
     CDS             complement(773..2293)
                     /locus_tag="PK171_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05496.1"
                     /translation="MTYLKGAIYVSNTAPFIKKNPWDYISSITSQIEGESTIPLSDAD
                     IKVFMGGSSDLICRGLSSDEGEFYLRVPSGINCFIEVYMNQKLILIKHLQVSEKREMK
                     VGSLDIESTAEAILLKKKLLINQDQPLSPVLLEKLYPQIEYCWKNGISLHKLYDTQDN
                     HDQTVISKHDPFQLITSFNIEIDEGNENLFWSWVTREPCQARLFYRSFRSRHYRQVEF
                     PEYKKRGFHNLSSPQEFEGYVYYLEVQTQKGFLALTPPRCFRIPIKPRLARHRFIGRQ
                     EGPVVTTRNVTHGKKTIQLSDLKIDLLLKGAIEKSFESEMNLEFVKKIKIPRFILREG
                     FHELELNIYFPQDHSFLWGPIETPEELSIDSQTWEKIKKLWAKFQISAPSNSVVEIGY
                     SKPFSEIISYRNRSHYRLFSNMDLSDTILILSYRSLDTLNLDCMQFFLGQLNLQGEGQ
                     FSDYHLQLKLEGRFFEEKSNDLIIQSSRDIYGQIDLDADMVITANSYHQLNSFNPY"
     gene            complement(2696..>3279)
                     /locus_tag="PK171_04565"
     CDS             complement(2696..>3279)
                     /locus_tag="PK171_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05497.1"
                     /translation="SSVATDSSGRATVIYYAPDVSDVTTVSVTAEFRGVTGLGATTTT
                     ISGIIEPIFPITPTNTLYYFDFGNGAAEHNFLTMRYTGNLVNGFSLSDTYSLEILSGN
                     NVDLTFSPGSIPEKATLFIWAQGDSGARVRVTFNNRNPLNINLDSSIIEPDNPKVVNI
                     PIQSLVEGNNRLRIEGDAARRRAIHIQRLVIVF"
BASE COUNT         1038 a          585 c          637 g         1019 t
ORIGIN      
        1 cccacaaagg ggcgaagaaa aaaataaata aaaaaatgaa atagttcatt ctcacttgat
       61 gagagaaggc aaaagtaaaa tctaaccagg gagctgaaat aaatatgatc aaagggtcta
      121 aaatttagta acgagaggta aaatatgata caagaaacag cgatgaagat tatcgatatc
      181 cttgaaaaac gatttcccga tgcatgttta ctcctcaatt atagaaatgc ttttgaactg
      241 ctggttgtaa caattttagc tgctcaggct ccggatgaaa gggtcaatca agtaagccct
      301 tccttattcc aaaagtatcc caatccggct gctatggctg aagcagatga tgaggaaatg
      361 gataaaataa ttcaaactat tagtttttat cggcaaaaag gacgtaatat ccgagcaatg
      421 agcaagaaga taattaaaga tttccaggga gaagttccta aaaaagtgga agaattggtt
      481 cagctcaatg gtgtggggag aaaaactgct aatattgttt tagccaatgg ctttggcatt
      541 ccagctatac cagttgatac tcatgtagca agggtttctc aaagactggg ttggtctgaa
      601 ttttcaaatc cggatcaaat tgagtcggat cttgctaaga ttttccctcg agagaaatgg
      661 ataagagtaa cacacctctt aggattttta ggccgatata tctgtcaagc aaagaaacca
      721 aaatgctggg aatgcccagt taccaattgg tgtccttatc cgggaaaaac cattagtaag
      781 gattaaaaga atttaattga tgataagaat ttgccgtgat gaccatatcg gcatccaggt
      841 caatctgacc ataaatatcc ctggaacttt gtatgataag gtcattgctt ttttcttcaa
      901 aaaaacgacc ttctaacttc agttgaaggt ggtaatctga aaattgacct tcaccctgaa
      961 gattaagctg ccccaggaaa aactgcatac aatccagatt aagtgtatct aaagatcgat
     1021 aactgagaat gagtattgtg tcagacaagt ccatattgga gaaaagccga taatgacttc
     1081 gattccgata ggaaataatt tcagaaaagg gtttgctata accaatttca acgacactat
     1141 tggaaggtgc actaatctga aattttgccc ataatttttt tatcttttcc caggtttgag
     1201 aatcgataga aagttcttcc ggagtttcaa tcggtcccca gagaaatgaa tgatcttggg
     1261 gaaagtagat gttgagttcc agctcatgaa acccctcacg aagtataaag cgcggtattt
     1321 ttattttttt aacaaattcc aagttcattt cagactcaaa actcttttct atagctcctt
     1381 tcagcaagag gtcaattttg agatcggata attgaatggt tttttttcca tgagttacat
     1441 tacgggtggt aaccaccgga ccttcctgcc ggccaataaa tcgatgtctt gccaatcgag
     1501 gcttgatagg aatacggaag caacgaggcg gtgtgagggc caaaaaacct ttttgagttt
     1561 gaacttccag atagtaaacg tatccttcaa attcttgagg agaagaaaga ttatggaagc
     1621 cccttttttt atattcggga aattcaacct gtcgataatg tcgagatcga aaggaacgat
     1681 aaaagagcct agcctggcaa ggttcccgag ttacccagct ccaaaataag ttttcgttcc
     1741 cttcatcaat ttcaatatta aaagaagtga tgagctggaa gggatcatgc ttagaaataa
     1801 cggtttgatc gtgattatct tgggtatcat ataatttatg aaggcttatc ccatttttcc
     1861 agcaatattc tatttgagga taaagctttt caagaaggac aggcgataag ggttgatctt
     1921 gattgattaa gagctttttt ttaagcaata ttgcttctgc ggtgctttct atatccaaag
     1981 aaccaacttt catttctctt ttttcactca cctgaaggtg cttaattagg attaattttt
     2041 ggttcatgta aacttcaata aaacaattta ttcctgatgg aacccgaaga taaaactcgc
     2101 cttcatcgct cgataatcct ctacatatta gatcagagct tcctcccata aagaccttaa
     2161 tatcagcatc gcttaacgga atggtggatt ctccttcgat ctgcgaggtg attgatgaga
     2221 tatagtccca aggatttttt ttaatgaaag gagcagtatt tgaaacatat attgctcctt
     2281 ttaaataggt cattttttca cccttttaat agagaaaaat ctttccaatt acttatactg
     2341 taaatatagt gagaagtgaa tttaaaaata atcaccctca tcctcacctt cttttatcaa
     2401 gggagaagga attattatga tacgtcatcc tgagacctca ctttgtgagg gcgtgaggat
     2461 ctcatctttt tatttttttt atccctttgg ataaaggaag atggttcggg attcgattct
     2521 tttcaaaaaa aactcatatc ccccttcata cttctttgct aaagggggat attgattggt
     2581 atacaattat tttcatcctc acttagtgct actaaagtgg taggaggatc tattcaaaaa
     2641 ataaaatact ccatgccgtt ttacttttgg tatggagtat tttataagaa taactttaga
     2701 agacgataac taatcgttga atgtgtattg ctcttcttct tgctgcatca ccctcaattc
     2761 gtaaacgatt gttgccttca actaagctct gtataggaat attaactact tttggattgt
     2821 caggctcaat gatactgcta tcaaggttta tatttaatgg atttctatta ttgaaagtaa
     2881 cacgaactct tgctccagaa tctccttgag cccagatgaa gagtgttgct ttttctggaa
     2941 ttgatcctgg cgagaaagtt aaatcaacat tatttccact gagaatctca agtgaatagg
     3001 tatcgcttag cgaaaaacca tttaccaagt tcccggtata tctcatggtt aagaaattat
     3061 gttctgctgc tccatttcca aaatcaaagt aataaagagt gttcgttggt gtgatgggga
     3121 atattggttc aattataccc gagatggttg ttgttgtagc tcctaatccg gttacacctc
     3181 ggaattctgc agtaacagaa actgtagtta catcacttac atctggagca taatagatta
     3241 cagttgctcg ccccgatgaa tcggtcgcaa ccgatgatt
//
LOCUS       DAOSOL010000159         3256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_29563_length_3256_cov_4.555764, whole genome shotgun sequence.
ACCESSION   DAOSOL010000159 DAOSOL010000000
VERSION     DAOSOL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29563_length_3256_cov_4.555764"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PK171_04570"
     CDS             <1..465
                     /locus_tag="PK171_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05498.1"
                     /translation="LVFFSIIFFKNNVLTQYWPILGQLSFLPFMIWAVCVSEKRWYFA
                     LISCLLVAFLLEFFSFSIFSFHILLSIGLFLLAQTWISFFSITSGSLLGFCLTASGVE
                     IFFIIVLQYFTRSVQHSWLFSVELLALSFFLNLLVLSAWLYYFPPERLNNVE"
     gene            455..2215
                     /gene="mrdA"
                     /locus_tag="PK171_04575"
     CDS             455..2215
                     /gene="mrdA"
                     /locus_tag="PK171_04575"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOT05499.1"
                     /translation="MWNEKPKIVINHRIELFTWILFGIFVFLFFNLWYLSVLQSGLYQ
                     EKSERNRIRMIPLQAPRGIITDRYGQILAQDVPRFAAVLLPGSADPEKSRQILEKTIK
                     RKIEEPLRDRYSGEIILASQMSIEEVAMVEETRKELPGVMIEAYPKRIYPPGEDIAHI
                     VGYVGKINSEELKKFGQTNYNNEDVVGKSGIELNYEEFLRGQKGYRKIEVDALGRIVR
                     ILDTSFPRFRHTLTLTLDLELQRYCNELLAGKSGVIIVGDPTNGELLAMANQPSFEPN
                     LLIDGVSQDEWNELIENPQNPLTTRSLQALYPPGSIFKLIVGLAAQEANIVSRERTIF
                     CPGYYEYNNRRYPCWRKAGHGRLNYEDALAQSCNVTFYTLGLELGQDRIIDMARRLGF
                     EEDTGIDLPGENHGFLPTSRWKRQKIGEPWYPGDTINLSIGQGYLLVTPFEIFQSVVA
                     ISNRGVVYTPHFLKVVQNDKREVIERTLPKVKNRIELKPDIWERLIRGMTKVVVEGTG
                     RACRDLPIPIAGKTGTAQNPHGNDHSWFAGFFPIDNPRYVFVVLVENGGDGSGEAAHR
                     SKDLIQWIYDHRENTLNENG"
     gene            2202..>3256
                     /gene="rodA"
                     /locus_tag="PK171_04580"
     CDS             2202..>3256
                     /gene="rodA"
                     /locus_tag="PK171_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOT05500.1"
                     /translation="MKTVKMFPIIILSLNIVGLLAVYSTDPLRGEAVFIQTFFGRQLM
                     WSLLGWLVFWGISRIHYHWFIKLEWMIYLIIILSLIVVLFFGSGDDVGTRRWILFRSV
                     QPSEFSKVGMAVFLAAFLSAQPEDYGSWQLLLKTSFLSIIPTGLIFLEPDLGTALVII
                     VLWFSALFFCGFQWKKILAVFAGLVGLIPFFWLVLHDYQRKRLLSFFAPQSDPLGTGY
                     NVLQSKITIGAGSFFGKGWLSGTQSQLRFLPAHYTDFIFASWCEQWGFVGGVIILALF
                     FWLIWSIFRIGIETPDLEGKILTTLFGSMFVFQVMINVGMNMGVMPVTGIPLPFISYG
                     GSSFFINMLAMGMVWNIA"
BASE COUNT          871 a          547 c          716 g         1122 t
ORIGIN      
        1 ttggtttttt tttcaataat tttttttaaa aataatgtat tgacccaata ttggccaata
       61 ttgggtcaac tctccttttt acctttcatg atttgggcgg tttgtgtttc agagaaaaga
      121 tggtatttcg cactaatttc ctgccttttg gttgcatttc tgttggaatt tttttctttt
      181 tctatcttta gttttcatat tttattaagc attggccttt ttttattggc acagacttgg
      241 atttcctttt tctcgattac cagtggatcg cttttaggat tttgtctgac agcatctggc
      301 gttgaaatat tcttcattat agtcttacaa tattttaccc gaagcgttca acactcttgg
      361 ttgttttcgg tcgagctgtt ggctctttct tttttcttaa acctccttgt gctttctgcc
      421 tggttatatt acttcccacc cgagaggtta aataatgtgg aatgaaaaac caaagatagt
      481 tattaaccat agaatagaat tgtttacttg gattttgttt ggaatatttg tctttctttt
      541 ttttaatttg tggtatttga gtgtattgca atccggcctc tatcaggaaa aatctgagcg
      601 taaccggatt cgaatgatcc cgctccaagc gcctcgagga ataataaccg atcgatatgg
      661 tcagatcctc gctcaagatg taccgaggtt tgcagctgtt ttattacctg gaagcgctga
      721 tccagaaaaa agccgtcaga tattggaaaa aactatcaaa agaaagattg aagaaccgct
      781 ccgtgaccgg tattcgggag aaatcattct tgcttcccag atgagtatcg aggaggttgc
      841 tatggttgaa gaaaccagga aagagctccc tggtgtgatg attgaagctt atcccaagag
      901 aatttatccc cccggtgaag atatcgccca tattgttgga tatgtgggga aaataaacag
      961 tgaggaacta aaaaaatttg gccagaccaa ttataataat gaagatgttg ttggaaaaag
     1021 tggaattgag cttaattatg aagaatttct caggggtcaa aagggatatc gaaaaattga
     1081 ggttgacgcc ttggggagaa tagtacgaat tttagatacc agctttccac gatttcggca
     1141 taccttaact cttactttag atttggaatt gcaaaggtat tgcaatgagc tcttagctgg
     1201 aaagagtggt gtgattatag ttggggatcc gactaatgga gaattattag caatggcaaa
     1261 tcaaccctct tttgaaccta acctattgat tgacggggta agccaggatg aatggaatga
     1321 attaattgaa aatcctcaaa atcccttgac aacaagatct cttcaagccc tttatccacc
     1381 tggttcaata tttaaattaa tagttggttt agctgcccag gaagccaata tcgtttcaag
     1441 agaaagaaca attttttgcc ccgggtacta tgaatacaat aatcggaggt atccttgttg
     1501 gagaaaagct gggcatggaa gattgaatta cgaggatgct cttgctcaat cctgtaatgt
     1561 tacattttac accttaggtt tggaactcgg tcaagataga ataattgata tggcaaggcg
     1621 cttaggtttt gaagaggata ctgggattga tttaccgggg gagaatcatg gctttttacc
     1681 tacctctcgc tggaagagac agaaaatcgg agaaccttgg tatccaggag ataccataaa
     1741 tctttctatc ggacaagggt atcttttagt cactcctttt gaaattttcc aaagtgttgt
     1801 tgcaatttcc aaccgagggg tggtatatac tcctcatttt ttaaaagtcg tccaaaacga
     1861 taagagagaa gttatcgaac gaacactacc aaaagtaaaa aatcgaatag agttgaagcc
     1921 agatatctgg gaaagactga ttcgaggaat gaccaaggtg gtcgttgagg gaaccggtag
     1981 agcctgtcgg gatttgccaa tcccaatagc cgggaaaact ggaaccgccc aaaatcctca
     2041 tggtaacgat cattcttggt ttgctggctt tttccccatt gataatcccc gttacgtttt
     2101 tgtggtttta gttgaaaacg gtggagatgg aagcggagaa gcagctcatc gaagtaagga
     2161 tttaatacaa tggatctatg atcaccggga gaatacactt aatgaaaacg gttaaaatgt
     2221 ttcccatcat tattctttct ctcaatatag ttggtctttt ggcagtttat agtaccgatc
     2281 cccttcgtgg tgaagctgtc tttattcaga ccttttttgg aagacaactg atgtggagtc
     2341 tattgggctg gttagttttt tggggaatta gtcgtattca ttaccattgg tttatcaagt
     2401 tggaatggat gatttattta attataattc tctctttaat agtagttttg ttttttggaa
     2461 gtggagatga tgtaggaact cgacgttgga tattatttcg ttccgttcaa ccttcagagt
     2521 tttctaaagt tggaatggcg gtttttttag cagcgttttt gagtgctcaa cctgaagact
     2581 atggaagctg gcaacttctt ctaaaaacat cgtttttatc aattatccca actggtttga
     2641 tattccttga accggatttg gggacagctt tggtcataat agttctttgg ttttcggcgt
     2701 tatttttttg tggctttcaa tggaaaaaaa ttttggcggt ttttgctggt ttagtaggtt
     2761 taattccatt tttttggttg gttttacacg actatcaaag aaagcggtta ctttcttttt
     2821 ttgctcctca atctgatccc ctgggaaccg gttataatgt tctgcaatcg aaaattacta
     2881 ttggagcagg gagttttttt ggaaagggtt ggctttcggg aacccaatcc caactccggt
     2941 tccttcccgc tcattatacc gattttattt ttgcttcttg gtgtgaacaa tggggctttg
     3001 ttgggggtgt gattatcctt gctctatttt tctggcttat ttggtcaatt ttccggattg
     3061 ggatagaaac acctgattta gagggtaaga tacttactac tctctttggt tcaatgtttg
     3121 tatttcaagt gatgattaat gttggaatga atatgggtgt tatgccggtt accggtattc
     3181 ccttgccatt tattagttat ggtggcagct cattttttat taatatgtta gccatgggaa
     3241 tggtatggaa tattgc
//
LOCUS       DAOSOL010000160         3227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_29889_length_3227_cov_3.813682, whole genome shotgun sequence.
ACCESSION   DAOSOL010000160 DAOSOL010000000
VERSION     DAOSOL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29889_length_3227_cov_3.813682"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PK171_04585"
     CDS             complement(<1..291)
                     /locus_tag="PK171_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05501.1"
                     /translation="MKKFLLLIILISLFCFFKENVANGTQIFATPNATTETPQEETIK
                     SLQKKIDDVIPQINLSARSEQTKTAQIYGITLEELQIKTNKLRDLQITYQQHK"
     gene            563..1786
                     /locus_tag="PK171_04590"
     CDS             563..1786
                     /locus_tag="PK171_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOT05502.1"
                     /translation="MNPLSAFISLNDPQKEALGIQYTPQEIHDQVDLWTSSVERVFQY
                     RQRLIELFESLSGKKKSVIYLAGAGTSEFVGYCLQGIFRQSFPVPVQVVSTGKLVTHP
                     FEYFAHTNPLMISFARSGDSPESCGAYEIASQFSDSICHLVITCNKEGGLARRARHDP
                     NSLVIDLDEKTNDRGLAMTASFSNMVIAGQACSQIYSPDEYTQKLPQLVVAGRKVLAM
                     APEVVKKIAESDFQRAVFLGSGSHFGTAIESHLKLQELTSGQIMCGYNTFLDVRHGPK
                     AVIDKHTLVVAFLASDPYVRRYEAELLKEIRQQELGKMTILVGEGLDDEISRYGDEMI
                     DIKSDSQAALPDSLTPPVYVMIGQLLGLFKSLHLGFKPDSPSKTGVIHRVVQGVKVYD
                     PKIFKEKGIFQEIKG"
     gene            1887..2828
                     /gene="speB"
                     /locus_tag="PK171_04595"
     CDS             1887..2828
                     /gene="speB"
                     /locus_tag="PK171_04595"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOT05503.1"
                     /translation="MDFEKIDRSHFSYSDVPTFFKFPHSKELHNVDVAMVGVPLDQAT
                     TNRAGTRFGPRSIRLASQMYGAEFYDGEGIFDVELGRFILKEIRAIDYGDMPIFPTMT
                     ELNIQSIEKITKKIIEANVFPVIFGGDHSISYPIVKAFSNEKLDIIQLDTHLDFANDS
                     GGLGMIYSHGSPIKRISELGNIGQITQIGIRGLLNDKNTFLEAKKFGTKIITVQQVFE
                     KGLNWVIGEIPQSENMYVTIDIDVFDPSIAPGTGTPEPGGLSYFQVKQLLTALPTKGR
                     ILGFDLVEVNPLYDSGEITSQLAARITLDFLGAIMDQ"
BASE COUNT          964 a          592 c          694 g          977 t
ORIGIN      
        1 tttgtgctgc tgataagtga tttggaggtc tcttaattta ttggttttga tttgaagttc
       61 ttctaaggtt ataccatata tttgtgcagt tttggtttgc tctgaacggg ctgaaagatt
      121 gatctgagga attacatcat ctatcttctt ttgaaggctt ttaatggttt cttcttgagg
      181 ggtttctgtg gttgcgttgg gagtagcaaa aatttgtgtt ccatttgcaa cattttcttt
      241 gaagaaacaa aacaaggata ttaatattat aaggagaagg aactttttca tagatatcta
      301 atccattcct cttctaaggt ggattttgtt ttgttattgt acctttgaac cattgattcc
      361 acaagttttt ttatataaat ctaatagaat aaaagtatca atgaaatcaa tattttcctt
      421 gttgataaag atagatattt ttcaaaaccg gtagtgggct attctccaag ataaaaaact
      481 tcttatagga attatgattg gcatcgttta caatgaatga atgaaattaa tagaaaaaac
      541 ttagtgaaaa ggagaccaat atatgaaccc tttatctgca ttcatttccc tcaatgatcc
      601 ccaaaaagaa gctctgggta ttcagtacac accccaggag attcatgatc aggttgatct
      661 ctggacatcg agtgtggaaa gagtctttca ataccgtcaa aggttaattg agctttttga
      721 gtctctatct ggaaagaaga agagtgttat ttatttagcc ggtgccggta cgtcagagtt
      781 tgtcggatat tgtttgcagg ggatatttcg tcaaagtttt ccagttccgg ttcaagtggt
      841 ttcaaccggg aagctagtca cccatccttt cgagtacttt gcccatacca atccgctgat
      901 gatctccttt gcccgttccg gggacagtcc ggaaagttgt ggtgcctatg agatcgccag
      961 tcaattctcc gattccattt gccacctggt gattacctgc aataaagagg gtggtttagc
     1021 ccgaagagca cggcacgatc ccaacagtct ggtcatcgat cttgatgaga aaaccaatga
     1081 ccgcggatta gccatgacgg catctttcag taatatggtg attgccggtc aagcttgttc
     1141 ccaaatttat tcccctgatg agtataccca aaagcttccc caactggtgg ttgctggaag
     1201 aaaggtgctt gcaatggcac cggaagtggt taagaaaatc gctgaaagtg atttccaaag
     1261 agcggtcttt ttgggaagtg gcagtcactt tggaacagct attgaatccc acctcaagct
     1321 ccaggagctc actagtggac agatcatgtg tgggtacaat accttccttg atgtacgcca
     1381 cggaccaaaa gcggtcatcg ataaacacac gctggtcgtc gcttttcttg cttctgatcc
     1441 ctacgttcgc cgttatgaag cggaactctt gaaggaaatc cgacagcagg agttgggaaa
     1501 aatgaccatt ctcgtcggag agggactgga tgatgaaatc agtcgctatg gagatgaaat
     1561 gattgatatc aagagtgatt cccaagcagc tcttcccgac tctcttactc caccagtcta
     1621 tgtcatgatt gggcaattgt tgggtttatt taaatctctt cacctcggtt ttaaacccga
     1681 ttcaccgagc aagaccggag ttattcatcg agtggttcaa ggagtgaagg tttacgatcc
     1741 aaaaatattt aaagaaaaag ggatatttca agaaatcaaa ggatgagcgt aagtcaaaaa
     1801 aagaaaggat tggtacttaa agatttaaaa atcataaaag ttcattttta gaaaaagatg
     1861 agttaaaaat acgagaaggt gctgttatgg acttcgagaa aattgataga agccattttt
     1921 cctatagtga tgtaccaact ttttttaaat ttcctcactc aaaagagctg cataacgtag
     1981 atgttgccat ggtaggagta cctttggatc aagctactac caatcgagca ggaactcgtt
     2041 ttggtcctcg ttcgattcgt ttagcatcac agatgtatgg ggctgaattt tatgatgggg
     2101 aagggatttt tgatgtcgaa ttgggacgat tcatcctcaa ggaaataaga gctatcgatt
     2161 acggtgatat gccaatattt cctaccatga ccgaattaaa tattcaatca attgagaaaa
     2221 taaccaaaaa gataattgaa gcaaatgttt ttccggtaat ttttgggggc gatcattcca
     2281 ttagttatcc aattgtcaaa gcattttcga atgaaaaatt ggatatcatt caattggata
     2341 cccatcttga ttttgccaat gatagcggtg ggttagggat gatctattcc catggtagtc
     2401 caatcaagag aatttcagaa ttagggaata ttggtcaaat tacccagatc ggtattcgag
     2461 gtttgctcaa tgataaaaat actttcttgg aagcaaaaaa attcggcaca aaaatcatca
     2521 ctgttcaaca ggtttttgaa aaaggtttga attgggtcat tggagaaatt ccccaatcag
     2581 aaaatatgta cgtcactatc gatatcgatg tatttgaccc ttcaattgca cccggcaccg
     2641 gaacgcccga accgggggga ttgagttatt tccaggtaaa gcagcttctt acagctttac
     2701 caaccaaagg tcgaatcctt ggctttgatc tggttgaagt caatccatta tatgattctg
     2761 gagaaataac gtctcagtta gcagctcgaa ttaccttaga tttcttagga gcaataatgg
     2821 atcaatgatc cattattgct ctattccacg gatgaagcat ttcctttcca taggcatcca
     2881 ggctactttg tgactccagt taaggattaa aaccaagatt cgacaggcca tggcatgcca
     2941 ctacattaat taaagaatgg gcacaataca ttgtgcccct acaattttat actcatttgt
     3001 aggggcttga tttatcctgc ccgggggttt tgaggttaga tcctcatgct aatttgatgg
     3061 caccagatga ggatgagatt actcattcag aaatcaatct cccactttag aaaagagagt
     3121 aaggggggat ttgataaaaa ctgaaaaaaa tcgaatccct cttaatctcc ctttatccaa
     3181 agggagagaa taaaaaagaa tgggtagatg agattgccac gtcgctg
//
LOCUS       DAOSOL010000161         3221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_29964_length_3221_cov_3.373342, whole genome shotgun sequence.
ACCESSION   DAOSOL010000161 DAOSOL010000000
VERSION     DAOSOL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29964_length_3221_cov_3.373342"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1902
                     /locus_tag="PK171_04600"
     CDS             <1..1902
                     /locus_tag="PK171_04600"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05504.1"
                     /translation="VKANTVDEFGESVINFKTSSPFAFRVELPPFLSQGDSIETQMTL
                     WNEYRQQKVQLEAQTGSELKIGTYPHEVVVDTNQNLVIPLQFQALQAGVPFVRMYARG
                     EVSGDAIEVTQKVKPRGIEIEQFFTTFVSDGKGRLGFTYQPEMFNHPPQVECILYRNL
                     NGVFYQIMEDMYYYPYGCTEQTSSRIISLLSLPLPDNLEWTKTVPREIQEGIYQLYAL
                     QHYDGGWGWWNLDDSNVFNTSHALLALITAREKGYLVSENSILQAKSYLMSINKDLVP
                     WEKAMSYYVLSQDGEKITDLTSFEIDKRLERMDVKELAWLSLVAQGKAQQSLLKELIK
                     RAQPIGDAVFYGYSNPEKPWEDDRFLATCWATLALNKENRSIAEKSFLWLLRNIQSEW
                     YINTIEKAYFMKLALLFQEESMVSKTRYQIMLNSEKTTEGQFESDSFSKKIDLNGEKL
                     LSGENQLELFGEGVYLMTVKIRGFTSGLPPSSQIKVSKNYWKLKPIKNEEGNFIFTKE
                     AISSLQPGDELVCEILIESPHQFDNLVIEDGMAAGFELIRNDYVYQLQDDRAHNNSDF
                     PIYVNKKIYDQLPVLFVSRIEEGENMIRYYLRVRDAGSFYVRPTQAYLMYFPEVRGYS
                     KEHEFTSGR"
     gene            1880..2545
                     /locus_tag="PK171_04605"
     CDS             1880..2545
                     /locus_tag="PK171_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05505.1"
                     /translation="MSLPVEDEYHCSKRHLALATVIFLLIIISTPVWCLSTTYIALSG
                     QLQYPQFEMERCLERAADTVHFYTGLNTEINIRIILHPDGFTFRSHAFPSWSAAVYRE
                     GEIHLRNPHHLLKKGILENVLSHEFFHAVIRQNGLFLPQWFEEGFAYFLFPASLQELN
                     GKMNDEDSSKIKALDFYSDSYIMVRKLMEGKSQREIQDFFLFCKEVDFYTASQKYFKI
                     SVY"
     gene            2570..>3221
                     /locus_tag="PK171_04610"
     CDS             2570..>3221
                     /locus_tag="PK171_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOT05506.1"
                     /translation="MNYATTLLLIFFLIFINAFFAASEIAVISLRRSKLKLLESDKSK
                     QVETVNRLLKNPSQFLATIQIGVTMAGFFASATGAVSLSHNLGRKLEATGLPILGRFG
                     EEIALIAVTLVISYLTLVFGELVPKRIAMSNPEALSIRLAKPLEYLLIAFKPMALILS
                     ASTDFISRLFGITHIDHSTHTDEELRIFITEQRTVPIEEKVLINEVFEFGDTMAYEV"
BASE COUNT          983 a          622 c          660 g          956 t
ORIGIN      
        1 gtcaaagcta ataccgtcga tgaatttgga gaatcggtta tcaactttaa gacttccagt
       61 ccgtttgcct tcagagtcga gttaccccca tttctatctc agggtgattc aatcgagacc
      121 caaatgaccc tttggaatga atatcgacag caaaaagttc aactcgaagc acaaaccggt
      181 tctgaattaa aaattgggac ctatcctcac gaagtggtcg ttgataccaa tcaaaattta
      241 gtcattcctt tgcaattcca agctctgcaa gctggagtac catttgtcag aatgtatgct
      301 cgcggagaag tatcgggcga tgccattgaa gtcactcaaa aggttaagcc acgtggtatt
      361 gagattgaac aattttttac aacttttgtc tccgacggaa aaggtcgact cggtttcact
      421 tatcaaccgg agatgtttaa tcacccgccc caagtagaat gtatattgta tcgaaatctc
      481 aatggagttt tttatcagat catggaagat atgtactatt atccctatgg ttgtactgaa
      541 caaacttcta gtagaattat ctctctcctt tctttgcccc ttcccgataa tttagaatgg
      601 accaaaaccg tacctcggga aatccaggaa ggaatttatc agctctatgc tttgcaacac
      661 tatgatggag gttggggctg gtggaacttg gatgacagta atgtttttaa tacatcccat
      721 gcccttctgg ctttaattac ggctcgggaa aagggatatt tggtttccga aaattccatt
      781 ctccaggcaa aatcctattt aatgagcatc aacaaggacc tggttccttg ggagaaagcc
      841 atgagctatt atgtgttaag tcaggacgga gagaaaatta ccgacctgac ttcttttgaa
      901 atagataaaa ggttagaaag gatggatgta aaagagcttg cttggctaag cttggttgcg
      961 caaggaaaag cccagcaaag cttactgaaa gaactgataa aaagagcgca accaattgga
     1021 gatgcggtat tttatggtta ttccaatccg gaaaaacctt gggaagatga tcgattctta
     1081 gcaacctgtt gggcaaccct ggcattgaat aaggagaata gatccattgc tgaaaagtcc
     1141 ttcctttggt tgctgcgcaa tatacaatcc gagtggtata tcaataccat agaaaaagct
     1201 tactttatga aactggctct gctatttcaa gaagaatcca tggtttctaa aacccgttat
     1261 caaatcatgc tgaactcgga aaaaaccaca gaaggtcaat tcgagtcgga tagctttagt
     1321 aaaaaaattg atttgaatgg agaaaagtta ctttctggag aaaaccaact cgaattgttc
     1381 ggagaagggg tttacctcat gacggttaaa ataagaggtt ttacttccgg cttgcctcct
     1441 tcatctcaaa tcaaagtgag taaaaactat tggaaactca aaccaataaa aaacgaagag
     1501 ggtaatttta tatttaccaa agaagcgatt tcatcactac aacccgggga tgaattggta
     1561 tgcgagatac ttattgaaag cccccatcaa ttcgataatt tggttataga agatggtatg
     1621 gcagcaggct ttgaattgat tcggaatgat tatgtttacc aacttcaaga tgaccgagcc
     1681 cataataatt ctgatttccc aatttatgtt aataagaaaa tttatgacca gcttccggtc
     1741 cttttcgttt cccgtattga agaaggggaa aatatgataa gatactatct aagggtccgg
     1801 gatgcaggct cattttatgt acgacctacc caagcctatt taatgtattt cccggaagtg
     1861 cgggggtact ctaaagagca tgagtttacc agtggaagat gaatatcact gtagcaagcg
     1921 tcatttggcg cttgctacag tgatattttt acttattatc atatcaacac cggtttggtg
     1981 tttgagtacg acctatatag ctctttctgg tcaattacaa tacccacaat tcgagatgga
     2041 acgatgcctg gaaagggcag ctgataccgt ccatttttat acgggtttaa atacagaaat
     2101 aaacatacga atcattcttc atcctgatgg tttcactttc cgtagccatg cctttcccag
     2161 ttggtcagct gcagtatatc gagaaggcga gattcatctt cggaatcccc accatttact
     2221 caaaaagggt atattagaga atgttctatc tcacgagttt ttccatgctg ttattcgcca
     2281 aaacggcctt tttcttcctc agtggtttga agaaggtttt gcttatttcc tttttcctgc
     2341 ctcgctacaa gagttaaacg gcaaaatgaa tgatgaagac tcctctaaaa ttaaagccct
     2401 ggatttttat agcgattcct atatcatggt taggaaatta atggaaggaa aaagccagag
     2461 agaaattcag gattttttcc ttttttgtaa agaggtggat ttttacaccg cctctcaaaa
     2521 atattttaag atttctgtct attgaagaaa ggagcaaaaa ggagaataaa tgaattatgc
     2581 aacaacatta ctccttatat tctttttaat atttattaat gcattttttg cagcttcaga
     2641 aatagctgtt atatctttgc gacggagcaa gttaaaactc ctggaaagcg acaaatcaaa
     2701 acaagtcgag acagtaaatc gattgttaaa aaatccgagt caatttttag ccaccatcca
     2761 aattggcgtg acaatggctg gattttttgc cagtgcaacc ggtgcggttt cgctgtcgca
     2821 caatcttggg agaaaattag aagctaccgg gttaccaata ctgggtcgtt ttggcgaaga
     2881 gatagcattg attgctgtta cgctggtcat ctcctacttg accctggtat ttggagaact
     2941 ggttcccaaa cggatagcca tgagcaatcc agaagcctta tcgattcggt tggcgaaacc
     3001 cttagagtat ttactcattg cctttaagcc gatggcactt atactttcgg catcaaccga
     3061 ttttatttcc cgtctgtttg gaattaccca tattgaccat tcaactcata ctgatgaaga
     3121 gctccggata tttatcacag aacaacgaac cgttccaatt gaagaaaagg ttttaatcaa
     3181 tgaagtattt gagtttggag ataccatggc ttatgaagtc a
//
LOCUS       DAOSOL010000162         3204 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_30128_length_3204_cov_3.303906, whole genome shotgun sequence.
ACCESSION   DAOSOL010000162 DAOSOL010000000
VERSION     DAOSOL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30128_length_3204_cov_3.303906"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PK171_04615"
     CDS             complement(<1..242)
                     /locus_tag="PK171_04615"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT05507.1"
                     /translation="MKTTRLIAYSGLSVAVVTVVTMVVQVPIPQTKGYINLGDAVILV
                     FAMLFGAKVGMIGGGLGSALADILTGYAHWAPFTLI"
     gene            complement(329..1171)
                     /locus_tag="PK171_04620"
     CDS             complement(329..1171)
                     /locus_tag="PK171_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05508.1"
                     /translation="MSPLMNYTRRRKVKNIILDLLIALVVIICVFPFIWMFMTSIKTR
                     VQTIDPSIWFFQPTLENYRAIFQKRDMFMYINNSIIVVLFTTLVSVALGTFAAYGLAR
                     FQFNRKEDIAYWVLSLRMLPPMAVVIPFFLLGRFIGLLDTHLLLIIVYLSFNIPFTIW
                     MMRGFIEDIPRELEEAAWVDGCSRFQAIRRIIFPLIAPGVAATSIFCVIQSWNEFSLA
                     FFLTSFNARTIPTTVTFFLSVLGVIWGEMAAVGIVATIPVLIFALIVQKYLVRGLTFG
                     AIKS"
     gene            complement(1172..2053)
                     /locus_tag="PK171_04625"
     CDS             complement(1172..2053)
                     /locus_tag="PK171_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05509.1"
                     /translation="MKNKKKEHYWYLLPSLLTLVAIVIFPTIFLWYMGFTNYDLTMGW
                     EQHKLIGIKNFQYLAFEDKDFWHSMKISLWFMVFTVVIEFVLGLAIALLFNRRIRGKR
                     LWMSFLIIPMVITPTIISLIWKLMLNTEYGVLNYILSLFSIAKINWLGYENALWSVMM
                     VDIWEWTPFVALILYAGLQSLPQEPYEAVVVDGASPLQIFYYLTLPLLKPMIFIAILL
                     RSIDSLKIFDIVYGLTQGGPGNATELMSMHIYRLGFRHTNWIGRASANAMVLLVIITI
                     LTNILLRIMRRGTREEF"
     gene            complement(2104..>3204)
                     /locus_tag="PK171_04630"
     CDS             complement(2104..>3204)
                     /locus_tag="PK171_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05510.1"
                     /translation="EGLLRQKLLLEHEAKTGVYDVILIDAFNMAEYAPSGVALDLQPL
                     LDNKEITPDWYDYADILPAYRDGIGTFNGVISGIPVAGETRYVGYRTDLFEKYGQKPP
                     ESMDDLLAMAKFFKGKEENLYGIGMRAQRGIHFASGWLTVMYQLGGQILDQKTWDVLL
                     NDPKTVASLEYFVDLLNQGPPDIAVYTHEEAVSVFTSGRAAMWFDSTALTPWVIDPTK
                     SAITDKVGFVPPPSGPAGAYGVLAGWNVGISSDVDAKRQEAAWAFIIWMTSKYKAKDY
                     VKLGGTPVRESVYTDEELVAANWTFPIQLESLGRAANLVKDDMMWLPPHPKLMKVLEI
                     AGSYGSDVLAGQISAKDAMDKAQAECVNIMAE"
BASE COUNT         1065 a          708 c          596 g          835 t
ORIGIN      
        1 ataataagag taaacggcgc ccagtgtgca tatccagtaa gaatatcagc taaagctgag
       61 cccaatccac caccgatcat ccctactttc gcgccaaaaa gcatagcaaa aactaaaata
      121 accgcgtcac caagattaat ataccccttg gtttgcggta tcggaacttg cacgaccatg
      181 gttacgactg tcacgactgc taccgataac cccgaatagg cgatcaatct cgtggttttc
      241 atttcatctc caccttaaga atatcttcat tcatattcta ttcctcggag agtaccattt
      301 caaggtacaa tagttcttta ttagtttctc aactcttaat ggcgccaaag gtaagaccac
      361 gaaccagata tttttgaact attaaagcga agataagaac tggtatggtt gcaactattc
      421 caaccgccgc catttctccc cagattacac caagtactga aaggaaaaaa gtgactgtcg
      481 tgggtattgt tcgagcatta aagctggtta gaaaaaaagc cagagaaaat tcattccagg
      541 attgaatgac acaaaatatt gaagtggcag caacccccgg agcaatgagt gggaaaataa
      601 tccgtcgtat agcttgaaac cttgaacagc catccaccca agccgcctct tccagctcac
      661 gaggaatatc ttcaataaac cctcgcatca tccaaatagt aaaaggaata ttaaaactta
      721 aatacacaat aatgagaagg agatgggtat caagaagtcc aataaaacgc cccagaagga
      781 aaaagggtat aaccactgcc atcggtggta acatacggag tgaaagaacc caataggcta
      841 tatcttcttt tctattgaac tgaaaacgag ccaacccata ggcagcaaaa gtgcctaatg
      901 ctacagaaac caaggtagta aataacacca caatgatact attgttgata tacataaaca
      961 tatcccgttt ttgaaatatt gcccgatagt tttcgagagt gggttggaaa aaccaaatcg
     1021 aaggatcaat ggtctgtacc cgagttttta ttgatgtcat aaacatccag ataaaaggaa
     1081 aaacacagat aataaccacc aaggcaataa gaagatctaa aataatattt ttgacctttc
     1141 ttcgtcttgt gtaattcatt aatggtgaca tctaaaactc ctccctggtt cctcttctca
     1201 ttatccgaag caaaatattc gtcaatattg taatgataac cagcaaaacc atagcattcg
     1261 ccgatgctcg tcctatccag ttggtatgtc gaaaaccgag tcgatagata tgcatactca
     1321 ttaactcagt ggcatttccc ggccctcctt gagtcaaacc atagacaata tcaaagattt
     1381 ttaaagagtc tatggaacga agtaaaatag caataaaaat catcggcttt aaaagaggaa
     1441 gggtaagata ataaaaaatt tgcagaggtg aggcaccatc aacgaccact gcttcgtagg
     1501 gttcttgagg gagagattgt aaaccggcat atagaataag tgcaacaaat ggagtccatt
     1561 cccaaatgtc aaccatcatg actgaccaga gagcgttttc atatcctaac cagttaattt
     1621 tcgcaataga aaataaagaa agaatataat taagaacgcc atattcggta ttgagcatca
     1681 atttccagat gagcgaaata atagtggggg tgatcaccat gggtatgatt aaaaaagaca
     1741 tccacaatct tttccctcga atacgacgat taaaaagtaa agcaatagcc aaacccaaca
     1801 cgaattcaat aacaacggta aaaaccataa accagaggct aattttcatc gaatgccaaa
     1861 aatctttgtc ttcaaaagcc agatactgaa aatttttgat tccaattaat ttgtgttgtt
     1921 cccatcccat cgttaaatca taattggtga aacccatata ccagagaaat atagtgggaa
     1981 aaattacaat cgccaccaaa gtaagcagcg agggcaataa ataccaataa tgttctttct
     2041 ttttattctt catcgatatt tctccagatc ggggagggca ttgtccctcc ccgatagtaa
     2101 ttcctattca gccataatat ttacgcattc agcttgggct ttatccatgg catctttggc
     2161 agatatctgt ccagctaaca cgtcggaacc ataggaaccg gcaatttcta aaactttcat
     2221 tagttttgga tgtggaggta accacatcat atcatcttta actaaattag ctgctcggcc
     2281 taaagattct aactgaatcg ggaaggtcca gttagcggca accagctctt catcagtata
     2341 aaccgattct cgtactgggg taccgcccag ctttacatag tctttcgctt tatatttgct
     2401 ggtcatccaa atgatgaacg cccaagcggc ttcctgccgt ttcgcatcaa catcactgga
     2461 gataccaaca ttccacccag ctaaaactcc ataagcaccg gctggtccac tgggaggtgg
     2521 cacaaaaccg accttatcag taatagctga tttggttgga tcaataaccc aaggagtaag
     2581 agcagttgag tcaaaccaca ttgctgctcg gccagaagta aaaactgaaa ctgcttcttc
     2641 atgggtataa acggcaatat caggaggccc ttgatttaaa agatccacaa agtactcaag
     2701 agaagcaact gtttttggat cattcaagag gacatcccag gttttttggt cgaggatttg
     2761 accacccagt tggtacataa ccgtcagcca accggaagca aaatgaattc ctctttgagc
     2821 tcgcatccct ataccataga ggttttcttc cttccctttg aaaaacttgg ccattgctaa
     2881 taaatcatcc atggattcgg gaggtttctg gccgtatttt tcaaaaagat cagtccgata
     2941 accaacataa cgagtttcac cagctacagg aattccactg atgactccat taaaagtccc
     3001 aattccatcc cggtaggccg gcaagatatc ggcataatca taccaatcag gtgtaatttc
     3061 tttattatca agaagtggct gtagatcaag ggctactcca ctaggtgcat actcagccat
     3121 attgaaagca tcgatgagta taacgtcata gacaccagtc ttggcttcgt gttctaatag
     3181 tagtttctgg cggagtaaac cttc
//
LOCUS       DAOSOL010000163         3198 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_30201_length_3198_cov_4.150493, whole genome shotgun sequence.
ACCESSION   DAOSOL010000163 DAOSOL010000000
VERSION     DAOSOL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30201_length_3198_cov_4.150493"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="rlmD"
                     /locus_tag="PK171_04635"
     CDS             complement(<1..1343)
                     /gene="rlmD"
                     /locus_tag="PK171_04635"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT05511.1"
                     /translation="MLKKGDVIEGIVETFALPDCVGVIRHQNWVVFVPGVLPGEKCRV
                     TIRKVKRNFLQGELEEFIEKSPARIEPICPHFKMGCGGCSLQFADYAEQVSIKEKNAL
                     NTIEKIGKVDLSSIDYEGFLPSVLNLGYRNKMEFNFGNQDGKLICGLRPKGKYWDLID
                     LQTCYLMNSQLVTGLLDFFRSYGNRYGLKGYDPVRKDGFLRNLLIRYNRSLDQYLIGL
                     STIRGELPGVGELVKNLTHYFPNVSGLVHIINNSPANALLFEEKILLYGSDYYIETIG
                     STQYKVSIDSFFQVNIHTGELLFPIITNYADLQPGESLFDLYSGNGSIGLYLSRQGTM
                     ITGIEENPQAVEDAQYNATLNSISSYNAICGRVEKALLDVIGNSMKKGIVDPPRAGLT
                     PKVIKTLIAAHPEKIVYVSCNTASLARDLAVFAEDNYFLKKISWIDLFPQTPHYEA"
     gene            complement(1337..2089)
                     /gene="rlmB"
                     /locus_tag="PK171_04640"
     CDS             complement(1337..2089)
                     /gene="rlmB"
                     /locus_tag="PK171_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT05512.1"
                     /translation="MSNIIYGRNPVKVAMESPRKKPIQLYLQNDIQGEVIIKIIELAN
                     RLKIPFIRLDKFAIQKIVGYPAHHQGVVLVYEPLGSPTWKDILTKAKSKKDSLVVFVD
                     SVEDPRNLGAILRTASAFGVDGVITSKAHTAPINSEVIKASAGELEKVDIAQENNFTG
                     IVKEFRENNFWMVSFEADAEMPLWEMDFSQGSWGIIVGGEDRGVRKIIRSLCDYVVSI
                     PLENMVNSLNVSVAFGIGLYEVIRQKQGSRSC"
     gene            complement(2238..>3198)
                     /locus_tag="PK171_04645"
     CDS             complement(2238..>3198)
                     /locus_tag="PK171_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT05513.1"
                     /translation="SRKPIEDQIEGARVEARYQKRNPADFVLWKSAKPGEPFWESPWG
                     KGRPGWHIECSAMSMKYLGETLDIHAGGIDLIFPHHENEIAQSEAATGKPFVKYWLHN
                     GYVNIQNEKMSKSLGNILTVRELYANRDPRLLRLVILFTHYRNPINIDDTILDAAGSY
                     LNRIKNFVRNFFFIYNQIQSDSKWQDSDKSLIDKIQSMYEKFVSALADDFNTPQALAV
                     LSDFIKEMNIYLQPKRRKWNLNTLESILGFLNQVNQILGVIPLQGIEEEDREVLKLIQ
                     DREIARKNRDFSQADLLRDTIVKSGYIIEDTPLGPRYYRDEDC"
BASE COUNT          972 a          676 c          548 g         1002 t
ORIGIN      
        1 gcttcgtaat gaggggtttg aggaaaaaga tcgatccagg aaattttttt taaaaaatag
       61 ttgtcctcag caaataccgc taggtcacga gccaacgatg cggtgttgca agagacataa
      121 acgattttct cagggtgagc tgcaatcaaa gttttgataa ctttgggtgt caaacctgct
      181 cgaggaggat caactattcc ttttttcata gaatttccaa ttacatccaa caatgccttt
      241 tccactcttc cacaaatagc gttataagaa gaaatcgagt ttaaagtagc attatactga
      301 gcatcttcaa ctgcctgggg attttcttca attccagtaa tcatggtccc ctggcgtgat
      361 aaatataatc caatacttcc gtttcctgaa taaaggtcaa aaagcgattc cccgggttgg
      421 agatcggcat aattggtaat gattggaaaa agtagctcac cggtatgaat attgacctga
      481 aaaaaactat caatggagac tttgtactgg gtcgaaccaa tggtttcaat ataataatcc
      541 gagccatata aaagaatttt ttcttcaaac aagagggcat tagccggtga attgtttata
      601 atgtgcacaa gtccagaaac gttaggaaaa taatgggtca agttcttaac cagttctccc
      661 accccaggga gttctcccct tatcgttgaa agaccgatca aatattggtc aagactgcgg
      721 ttgtaacgaa ttaataaatt tcttaaaaaa ccatcttttc gaaccggatc gtatcccttt
      781 aatccataac ggtttccata agatcgaaaa aaatctaata atccagtaac cagctgtgaa
      841 ttcattaaat aacaggtttg taaatcaata agatcccaat atttaccctt gggtctcaat
      901 ccacaaatca attttccatc ttggttacca aaattgaact ccattttgtt acgataacca
      961 aggtttagaa ctgatggaag aaatccttca tagtctatcg atgaaagatc gactttacca
     1021 atcttttcaa tggtattgag ggcatttttc tcttttattg atacctgttc agcataatcc
     1081 gcaaactgaa ggctacatcc accacagccc attttaaaat gaggacatat cggttcaatt
     1141 cgagctgggg atttttcaat gaattcttcg agttctcctt gaagaaaatt tcttttgact
     1201 tttcgaattg taactcggca tttttctcca ggtagaaccc caggtacaaa aactacccag
     1261 ttttgatggc gtatcactcc aacacaatcg ggaagagcaa aggtttcaac aattccttcg
     1321 ataacatcac cttttttcag catgaacgac tcccttgttt ttgacgaatc acctcataaa
     1381 gtccaatacc aaaggctact gatacgttta gcgagtttac catattttcc aagggtatag
     1441 aaacgacgta atcacaaagc gaacggataa tctttcgaac tcctcgatcc tctcctccta
     1501 ctataattcc ccaggaacct tggctaaaat ccatttccca taagggcatt tcagcatcag
     1561 cttcaaagga aaccatccaa aaattattct cgcgaaattc tttaacaatt ccagtaaaat
     1621 tattctcctg agctatgtcg actttttcca gttctcctgc tgaagcttta atcacttctg
     1681 aatttatcgg tgcggtgtga gctttgctag taataacccc atcaacacca aaagctgatg
     1741 ctgttctgag gattgcacct aaattccgtg ggtcttcaac gctatcaaca aaaaccacca
     1801 aagaatcctt cttggatttt gctttggtta gaatatcttt ccaggttgga cttcccaatg
     1861 gttcataaac caaaaccacc ccttggtgat gagctggata gccgacaatt ttttgtatag
     1921 caaacttatc cagcctaata aagggtattt ttaaacgatt ggctaactcg ataattttta
     1981 tgattacttc accttggata tcattctgaa gataaagctg tattggtttt ttccttggtg
     2041 attccatggc tacttttact ggatttcgtc cgtagatgat atttgacatt gcttcactct
     2101 tcttcaagct tattttgaat tcaccctcgg tcttgataaa tcaagcccca actaaagaat
     2161 ttaaaaagta ggggcgtaat tcattgcgca cgattaatgt agcgacatgc cgtggcaggt
     2221 tgaatctttg ttcttagtta acaatcttca tctcgataat agcgaggccc cagaggtgta
     2281 tcttcgatga tatagccgga tttcacaata gtatctcgaa gaagatctgc ctgggaaaaa
     2341 tctcgatttt ttctggcaat ttccctatct tgaatcagtt ttaaaacttc tcgatcttct
     2401 tcctctatcc cctgtagcgg tatcacaccc aaaatttggt tcacttgatt gagaaaacct
     2461 aaaatacttt ccaatgtatt gagattccat tttcgtcttt ttggttgaag ataaatattc
     2521 atctctttaa taaaatcact caaaaccgcc aaggcttgag gggtattaaa atcatcagca
     2581 agtgcagaaa caaacttttc atacattgac tggattttat ctattaagct tttatcacta
     2641 tcctgccatt tcgaatctga ctggatttgg ttataaataa aaaagaaatt ccgaacaaaa
     2701 tttttaattc gatttaaata actccctgca gcatctaaaa tcgtatcatc gatattaata
     2761 ggattgcggt aatgagtaaa aagaataact aaacgcaata atcggggatc acggttagca
     2821 taaagctcac gtacggtcaa gatattgcct aaagatttcg acatcttttc attttgaata
     2881 ttcacataac cattatgaag ccaatattta acaaaaggtt tgccggtagc tgcttcactt
     2941 tgagcaattt cattctcatg atggggaaat attaaatcaa ttccgccggc atgaatatcc
     3001 agggtttcgc ctaaatattt catcgacatc gccgaacact caatatgcca tcctggtctc
     3061 ccctttcccc aagggctttc ccaaaagggt tctcctggtt tggcgctttt ccatagaaca
     3121 aaatcggcag gatttctttt ttgataacga gcctcaaccc gtgctccctc tatctgatct
     3181 tctataggtt ttcgagaa
//
LOCUS       DAOSOL010000164         3190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_30300_length_3190_cov_4.002233, whole genome shotgun sequence.
ACCESSION   DAOSOL010000164 DAOSOL010000000
VERSION     DAOSOL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30300_length_3190_cov_4.002233"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="PK171_04650"
     CDS             complement(<1..404)
                     /locus_tag="PK171_04650"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOT05514.1"
                     /translation="MKPINEYILKTPLTIDDVLILHSGDKVLLNGEVYCARDATHRRM
                     IADIKSELEVPFPLMNSTFYYAGPSPAPPGKIIGSVGPTTSSRMDQFLEYFLQMGLRA
                     TIGKGERSPEIKKLFEKYSSVYFITCGGAAAY"
     gene            complement(410..1255)
                     /locus_tag="PK171_04655"
     CDS             complement(410..1255)
                     /locus_tag="PK171_04655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT05515.1"
                     /translation="MMKFISLEDLISRLESSLVEINTKCSEKLQNCMIEALTKEKSDA
                     AREMLSEILENYVIAQEECLPLCQDTGMVMAEVSMGIEVALTGGTFQEALDEAIRRAY
                     QKSYFRKSVLTDPLIGKNTGDNTPGIVFIQQTHGDELQVNLLVKGGGCDNISALKMMT
                     PGIAPDEIKKFILQELDKNASRACPPLIVGVGIGGNAAYALYLASRALGRPLGTPNHC
                     KEYRQWEEQWKNEINQLGIGPQGVGGKVTCLEVRIESYPTHIASLPVGMVCSCHVFRQ
                     KKLSW"
     gene            complement(1357..2586)
                     /locus_tag="PK171_04660"
     CDS             complement(1357..2586)
                     /locus_tag="PK171_04660"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOT05516.1"
                     /translation="MQKKTEKELNQPLIEAMKLHEFYQGKIEMNLKCPVRSYQDFAIW
                     YTPGVAEVCKAIVRDSMLSYEFTNRWNTVAVVSNGTRVLGLGNIGPEAAMPVMEGKAL
                     LFRYLGGVDSWPLCLKTQNTTELIQIVQSLEPSFGGINLEDISQPECFTVLETLQKSL
                     NIPVWHDDQQGTALVTVAGVLGAVEVVQKSLDKIRVAVIGAGASNIRIAHLLIKAGVL
                     AENIVLCDRSGTLHRERKDLEENHPWKWELCQITNGDNLKGGKAEAIANRDLLIALST
                     PGPGVIKPEWIQTMNKDAIVFACANPNPEIWPWEAKEAGAKIVATGRSDFPNQVNNSL
                     GFPGLFRGILSVQSACINDDMCIAASRAIYRFAQKRGLHTDYIIPSMEEWQLFIEEAL
                     AVAQVAIEQNLARKKRD"
     gene            2944..>3190
                     /locus_tag="PK171_04665"
     CDS             2944..>3190
                     /locus_tag="PK171_04665"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOT05517.1"
                     /translation="MPEATEYHQQGFNCCESTLMGLCDHLGKTCHFFPRLATGFGGGL
                     GHTGDVCGAITGSIMALGLEFGRDQAQDKKTRDDLYLL"
BASE COUNT          913 a          728 c          570 g          979 t
ORIGIN      
        1 aaataggccg ctgctccacc acaagtaata aaatacactg aactatattt ttcaaaaagt
       61 ttttttatct ctgggcttcg ttcaccctta ccgatagtcg cccttaaacc catttgaaga
      121 aaatattcca aaaattggtc catgcggctt gatgtcgttg gcccaaccga accaataatt
      181 ttgcctggtg gtgctggact cggaccagca tagtaaaaag ttgaattcat taaaggaaaa
      241 gggacctcta attccgattt gatatcagca atcattctac gatgtgtcgc atcacgagcg
      301 caatacactt ctccattcaa taacacctta tcaccactat gtaagattaa aacatcatca
      361 atagtcaaag gagtttttaa aatatattca ttaataggtt tcattctgat caccaagata
      421 acttcttttg acgaaaaaca tgacaggaac aaaccatacc aaccggaagg ctggctatat
      481 gggttgggta actctcaata cgaacctcta aacaagtcac cttgcctcct accccttgag
      541 gacctatccc cagttggtta atttcatttt tccactgttc ttcccattga cggtactctt
      601 tacaatgatt aggggtccct aagggacgcc cgagggcacg ggaagcaaga tacaaagcat
      661 aagcagcgtt tcctccgatt ccaacaccaa caattagtgg tggacaggct cggctggcgt
      721 ttttatccag ctcctgcaag ataaattttt ttatttcatc aggggcgatt cctggggtca
      781 tcatttttaa ggcactaata ttatcgcacc caccaccctt aaccagaaga ttgacctgaa
      841 gctcgtcacc atgagtttgt tgaataaata caatcccagg agtattatcc ccagtgttct
      901 ttccgattaa ggggtccgtt aaaactgact ttcggaaata gcttttttga taggctcgac
      961 gtatagcttc atccaaagcc tcttggaagg ttccccccgt caaggcaact tcaataccca
     1021 ttgaaacttc tgccataacc atgccggtat cctggcaaag agggaggcac tcttcttgag
     1081 caatcacgta gttttccaat atctcggaaa gcatttcacg agcggcatcg cttttttcct
     1141 tagttagggc ttctatcata caattttgta atttttctga acattttgta tttatctcaa
     1201 ctaaagagct ttccaatcgt gaaatcagat cctcgagcga aataaatttc atcattgctt
     1261 ctccagagga agaggaatat atccaaattt taccaaatct ttcaccatac cctgggctcg
     1321 taggataact ccttcaactt cttttcgcag ttcttctcaa tctctttttt tccgagcaag
     1381 attttgttca atagcaacct gagcaaccgc taaagcctct tcaatgaata attgccattc
     1441 ttccatactg ggaattatgt aatcggtatg aagccctctt ttctgcgcaa atcgataaat
     1501 agctcgggaa gctgctatac acatatcatc attaatacag gctgattgaa ccgaaagaat
     1561 accccgaaat aatcctggga atcccaacga attattcacc tgatttggga aatccgatcg
     1621 tccagtcgca acaattttag caccggcttc tttcgcctcc cagggccata tttccggatt
     1681 gggattggca caagcaaata caatagcatc tttattcata gtttgaatcc actcaggttt
     1741 aattacaccc ggacccggtg tcgacaaggc tatgagcaag tctcgattgg caattgcttc
     1801 agcttttcca ccttttaaat tatcgccgtt ggttatttgg cacaattccc atttccaggg
     1861 atgattttct tctaagtcct ttctttctcg atgaagtgtt ccgcttcgat cgcagaggac
     1921 gatgttttca gcaagaactc cagcttttat aagcaaatga gctatccgaa tattcgatgc
     1981 acccgctcct atcactgcaa ctcgaatttt atctaaggat ttttgtacga cttcaactgc
     2041 tcccaatacc cccgctaccg ttactaaggc tgttccctgt tggtcatcat gccaaaccgg
     2101 tatattcaaa cttttttgta aagtctctaa aacggtaaaa cattccggtt gtgaaatatc
     2161 ctcaagatta atccctccaa aactcggttc aagggattgg acaatttgga ttagttctgt
     2221 ggtgttttga gttttcaaac aaagaggcca agaatctact ccacccaaat aacgaaataa
     2281 aagcgctttt ccttccataa ccggcattgc agcttctggt ccaatattcc ccaaacccaa
     2341 aactcttgtt ccgtttgaaa ccaccgccac cgtgttccat cgattggtaa actcataaga
     2401 aagcatagaa tcacgaacta ttgccttaca aacttcggca actcctggag tataccatat
     2461 agcaaaatct tggtatgacc ttaccgggca ttttaaattc atttctatct ttccttggta
     2521 aaactcatga agcttcatcg cttctattaa cggttgattc aattccttct cggttttctt
     2581 ttgcattata aatctcctta ggtaaaaaac tattctttaa agacggtaat gaataaaaaa
     2641 catcctgagc ggtgcttttc cgcatgagga tctcatctgg tgacactata agcactaagc
     2701 accactgatt ttttaacggt tttcccaaat ttttaaacaa ctcatttgta aatttcattg
     2761 tcataataat aggcagtggt caacacattc gcaagagttc ttcatcgata aattctatca
     2821 tcaatttata ttttttatat tctgttatga gtttaccatt aattgaagta ggttttcatc
     2881 ctcagtgaaa tctgatagta ttaaaagaac tttaaaaaaa ttggtattag gaggcggtat
     2941 ggtatgccag aagcaactga atatcatcag caaggattta attgctgtga gtcaacatta
     3001 atggggttgt gtgaccattt aggaaaaact tgtcatttct tccctcggtt ggcaaccggt
     3061 tttgggggtg gcttaggcca caccggtgac gtttgtggag ccattactgg ttcaataatg
     3121 gctttgggct tagaatttgg acgcgaccag gctcaggata aaaaaacccg ggacgatctc
     3181 tatttattag
//
LOCUS       DAOSOL010000165         3176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_30466_length_3176_cov_4.247357, whole genome shotgun sequence.
ACCESSION   DAOSOL010000165 DAOSOL010000000
VERSION     DAOSOL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30466_length_3176_cov_4.247357"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..156
                     /locus_tag="PK171_04670"
     tRNA            78..156
                     /locus_tag="PK171_04670"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:114..116,aa:Asp,seq:gtc)
     gene            164..239
                     /locus_tag="PK171_04675"
     tRNA            164..239
                     /locus_tag="PK171_04675"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Phe,seq:gaa)
     gene            254..329
                     /locus_tag="PK171_04680"
     tRNA            254..329
                     /locus_tag="PK171_04680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Gly,seq:gcc)
     gene            346..420
                     /locus_tag="PK171_04685"
     tRNA            346..420
                     /locus_tag="PK171_04685"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:379..381,aa:Cys,seq:gca)
     gene            437..511
                     /locus_tag="PK171_04690"
     tRNA            437..511
                     /locus_tag="PK171_04690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:469..471,aa:Val,seq:gac)
     gene            929..2752
                     /gene="thrS"
                     /locus_tag="PK171_04695"
     CDS             929..2752
                     /gene="thrS"
                     /locus_tag="PK171_04695"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT05518.1"
                     /translation="MAKNAINSAIAAINKEGELVDLHESNGDIERYIFFDDKEGKSIY
                     WHSTSHLMAQAVKRLYPGARLAIGPAIEEGFYYDFDIDHTLNEEDLKKIEEEMQKIIQ
                     ENLTVERKVLSKEEAKQLFSSRGENFKIELIDEILDDQVSLYQQGEFIDLCRGPHIPS
                     TGSIKAYKLLSVSGAYWRGKETNPMLQRIYGIAFDDKDKLKNYLYRLEEAKKRDHRKL
                     GKELELFSLHEEGPGFPFFHPKGMVVINALLDAWRKEHVKRGYQEIKTPIILDRELWE
                     RSGHWDHYRNNMYFTSIDEREFAVKPMNCPGGILIYQSQLHSYREFPIRMAELGLVHR
                     HELSGVLHGLMRVRCFTQDDAHIYMEPHQVKQEIMGVIELADYIYRLFQFDYELELST
                     RPENSMGSDEMWDLATKSLETALQEKNLPYRVNEGDGAFYGPKIDFHLKDTLGRRWQC
                     GTIQLDFTMPECFDLSYIGEDGMKHRPVMLHRTILGSVERFLGILIEHTAGALPVWLS
                     PVQVVVLPIAERHYSYALEVKQQIAQAQVRVELNDENATLGAKIRKAQLQKIPYILIV
                     GDKEVENHQVSVRDRKEGDLDGFSLQDFLLRIQNEIQNFIM"
BASE COUNT          966 a          600 c          726 g          884 t
ORIGIN      
        1 caaaagcctt ttgacctatt aaaatgttaa ttgctttcgg gcgagggagg tgtcctcgat
       61 ccgaaataca gatgagcggg gtcgtggtgt agcctggcca aacacgtcag cctgtcacgt
      121 tgaagaccgc gggttcaaat cccgtcgacc ccgccaaatg agtgccgaga tagctcagat
      181 ggtagagcga tggactgaaa atccatgcgt ccccagttca attctgggtc tcggcaccat
      241 tgtagtttga gaggcggaag tagctcagtt ggtagagcgt caccttgcca aggtgagggc
      301 cgcgggttca agtcccgtct tccgctccag taaaatgaaa acttcggcgg catagccaag
      361 cggctaaggc agaggactgc aaatccttca tcctcggttc aagtccgagt gccgcctcca
      421 agaatataaa gtttgtgggc ggctagttca gaggaagaac gcttccttga cgcggaagag
      481 gccataggtt cgagtcctgt gccgcccacc aggaaacgtt attagggcgc cattccgggt
      541 taagagtggc gcttttttta tttttaaata tacaaggttt attggtccta tcccgtaaat
      601 acaggagcga gttataggta acgaatttag agtaactaaa ctgataatac acaatattta
      661 ataaatcccg actcaccttg tctgatcctc ccccaattta ttatattttc ttttctcaac
      721 ctttacaaaa ttggaggaag ttcgaggggg agattgctcg gtattaaatg tatttcatgg
      781 tcattagttg ctattattca gcctgagggt ctattgagaa atgctctaaa gtgttattat
      841 tttatgatat aaatttatat acaaaccgac aactttccca aagctactat aaattatctg
      901 gtgagatttt atgaaggagg aaaatgaaat ggcaaaaaat gcgatcaact cagcgattgc
      961 tgcaataaat aaagagggtg aattggttga tctccatgaa agtaatggag atattgaaag
     1021 atatattttt tttgatgata aagaagggaa atcaatttat tggcacagta cttctcattt
     1081 aatggcacag gcggtgaaaa ggctttatcc aggggctcga ttagccattg ggccagctat
     1141 cgaagaagga ttttactatg atttcgatat agaccatacc ttgaatgaag aagatttaaa
     1201 gaaaatagaa gaagaaatgc agaaaattat tcaagaaaat cttacggtag aaagaaaggt
     1261 tctttccaaa gaagaagcaa aacagctatt ttcttcgcgg ggggaaaact ttaagatcga
     1321 attgatagat gaaattcttg acgaccaggt cagcctttat caacagggag aatttattga
     1381 tctttgccga ggtcctcata ttccatcaac tggttcaatt aaagcctata agctcttgag
     1441 tgtatcagga gcctattgga gaggaaaaga aaccaatccc atgctgcaaa ggatttatgg
     1501 cattgccttt gatgataaag ataagctcaa aaattatcta tatcgcttgg aagaagcaaa
     1561 aaagagagat catcgaaaat tagggaagga gctcgaacta tttagccttc acgaagaagg
     1621 tccaggtttc ccctttttcc accccaaagg aatggtggtt atcaacgccc ttttagatgc
     1681 ctggcggaaa gagcatgtta aaagagggta tcaggaaata aaaactccta ttatccttga
     1741 tcgtgagttg tgggaacgtt caggacattg ggaccattat cggaataaca tgtattttac
     1801 ttcaattgat gaaagagaat ttgcagtaaa accgatgaat tgtccaggtg ggatcttgat
     1861 ttatcaaagc cagctgcata gttatcgaga atttccgata cggatggccg aattgggatt
     1921 agttcatcgc cacgaactct ccggggtcct tcatggtctc atgcgggtgc gatgtttcac
     1981 tcaagatgac gctcatattt atatggaacc ccatcaggta aaacaagaaa tcatgggagt
     2041 cattgagtta gcagattata tttatcggct ttttcaattt gattatgaat tagagctgag
     2101 tactcgacca gagaattcca tgggctctga tgagatgtgg gacttagcaa ccaaatcgct
     2161 ggaaactgcc cttcaagaaa agaacttacc ttatcgagta aacgagggtg acggagcttt
     2221 ttatggtccc aaaattgatt ttcacttaaa ggatactttg gggcgtcggt ggcaatgcgg
     2281 gactattcaa cttgatttta ccatgccaga gtgtttcgat ctcagttata ttggtgaaga
     2341 tggaatgaaa caccgaccag ttatgcttca tcgaaccata ttggggagtg tggaacgttt
     2401 tctcggaatt cttattgagc ataccgccgg ggctttaccg gtatggctat ctccagtgca
     2461 ggtggtggtt ttaccaatag ccgaaagaca ttattcctat gctctggaag tcaaacaaca
     2521 aatagctcaa gctcaggtac gagtcgagtt gaatgatgaa aatgctacct taggtgcaaa
     2581 aattcggaaa gcccagcttc agaaaattcc ctatatttta atcgtgggtg acaaggaagt
     2641 tgaaaatcat caagtaagcg ttcgagatag gaaagaaggg gatctggatg gattttcttt
     2701 acaggatttc cttcttcgca ttcagaatga aattcaaaac ttcatcatgt agcaccattt
     2761 aaagatttga ccagaaccga atgatcgttc atttgcagga aaaacattca tgccacttgg
     2821 tggtatcaga taagaatata aaatccgata ttctacatgc catggcaggt cgctacatta
     2881 gatgaagaac aggcacaata cattgcactc tccatttaaa attatttggt gttggtttga
     2941 tttatcatac ccgtgggttt tcaggattga tcctcaggtt gtctagcagc accaaatgag
     3001 gatgaaaata cctatctaaa tccgtcattg cgaggagccc aaccggtttt tggttggacg
     3061 acgtggcaat ctcatttatc atttttttaa acaattaaaa gatgagatcc tcacggcttc
     3121 aaaaaacgaa gcctcaggat gacggatgaa aggcacactt ctttgaatcc cccctc
//
LOCUS       DAOSOL010000166         3169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_30572_length_3169_cov_3.020231, whole genome shotgun sequence.
ACCESSION   DAOSOL010000166 DAOSOL010000000
VERSION     DAOSOL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30572_length_3169_cov_3.020231"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..500)
                     /locus_tag="PK171_04700"
     CDS             complement(135..500)
                     /locus_tag="PK171_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05519.1"
                     /translation="MSLLDKLEERSKKRERKSLYIPELEETVYWYPMTAGERQRIMNA
                     AGFKWARDAVQMDNAKYKASLIIEKLEDKDGKKIFSNTPEHKDLLINRIDDEFLSRIA
                     TAIDPPKSEEEQIEEAKND"
     gene            complement(514..1146)
                     /locus_tag="PK171_04705"
     CDS             complement(514..1146)
                     /locus_tag="PK171_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05520.1"
                     /translation="MIKKGYSAKVYGIDNSVAFSTAFTTEATTEGSNGDAKIYQINNT
                     GKRLWDPNEGITLDAGLDTPTLDESWKDRGIDWLTGRVKLNETGLTLTVSGKHFSTLV
                     EIGEAYNWTLDIAADVVDVSAFGDEWKKKTVTQKSWSGSFEKFAIDDYWFDIAKLGKV
                     FLVKLYTEATKGYQGFCVIPGLSSGASVADVLKETINFESHWLIEEFDEL"
     gene            complement(1161..1529)
                     /locus_tag="PK171_04710"
     CDS             complement(1161..1529)
                     /locus_tag="PK171_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05521.1"
                     /translation="MAVLKGYAGNIKVGTTTIGEMGDWSLDVNIDIADTSAFGDEWKK
                     KTGTQKDWSGSCVGRLDPNDAGQSQLTIGADVAMRFYEDNSHYYSGNAIVESISRSAT
                     VGGVLEVTFNFAGNGELSHV"
     gene            complement(1542..1967)
                     /locus_tag="PK171_04715"
     CDS             complement(1542..1967)
                     /locus_tag="PK171_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05522.1"
                     /translation="MKLSSLLKKIADQVETVTALADSAYLYNPNLDLSALTRPFAVVR
                     STINSEAVISFSDATRKHEYQIWVYVYPISNEYSALDLPKEVAAALKSELTVTEGTGE
                     TAKKYYAKILDLTIERLNGDEDDLERFGSVVSFVIRFYK"
     gene            complement(1964..2335)
                     /locus_tag="PK171_04720"
     CDS             complement(1964..2335)
                     /locus_tag="PK171_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05523.1"
                     /translation="MLNVTLRLEGIDKVIDSFGKRAGMVVQTVDFVTKKNAEEIRENA
                     KKRAPVLTGTLRGSLATEQKLPMVWGVGSYHPNVPYAKIRNYINKKNPQTVGYLTNSL
                     FEQRDKYQRDIKEAIKELKKL"
     gene            complement(2328..2726)
                     /locus_tag="PK171_04725"
     CDS             complement(2328..2726)
                     /locus_tag="PK171_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05524.1"
                     /translation="MTLITEAEKTAFKTTAGEIFTALNSVSWKTLTACECVDEYGIAD
                     PECEECGGDGFTESSKSIAADVQEITGDERVAVEAGILKRGDLIVRTLITNQVKVGDI
                     ITHSGKDYEVKFVRPDQIEAYLAVGCGKRA"
     gene            complement(2710..3069)
                     /locus_tag="PK171_04730"
     CDS             complement(2710..3069)
                     /locus_tag="PK171_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05525.1"
                     /translation="MTNLERLKKLIPSSSGYTDDELTAILSENNDSVYQAASEVIRGL
                     CSQALSGAFVFWSGEVKIDKSKIIDNYMKLAEKYEEKASSAPSSQDELWGQDLDRLSG
                     LDTTDYSDEDTEDDTNY"
BASE COUNT          832 a          748 c          569 g         1020 t
ORIGIN      
        1 catttcttca tgttgctctt tataaaatgc gatccagtcg tgcatttcac tttcggttaa
       61 ttcctcaata aactcataca caaagcgacc ctttttatca gcgagaacca ttaacaactt
      121 ataaaaagga tcgtttaatc gttttttgct tcttcgatct gttcttcttc gcttttaggc
      181 gggtcaatcg cagtagcaat gcggcttaaa aactcatcat caatacggtt aatcaataaa
      241 tctttatgct ctggagtgtt ggaaaagatt ttcttcccgt ctttatcttc caacttttca
      301 attatcaaag acgctttata tttcgcatta tccatttgaa ccgcatctct cgcccattta
      361 aaaccagccg cattcatgat tctttgccgt tctccagcgg tcatcggata ccagtaaacg
      421 gtttcctcta actctggaat atagagagat ttccgttctc tctttttcga tcgttcctct
      481 aacttatcta ataagctcat acaactcctc cttttacaac tcatcaaact cctcaattaa
      541 ccaatggctt tcaaagttaa tggtttcttt taaaacatca gctaccgaag caccgcttga
      601 gagcccagga ataacacaaa aaccctgata acctttagtg gcttcggtat agagttttac
      661 caaaaacact ttgcctaatt tggcaatatc aaaccagtag tcatcaatag cgaatttctc
      721 aaaacttccc gaccagcttt tttgggtaac ggttttcttt ttccattcgt caccaaaagc
      781 cgaaacatcc accacgtcag ccgctatgtc taaagtccag ttataggctt caccaatttc
      841 aactaaagtt gagaaatgct ttcccgatac ggttagggtg agtccggttt cattgagctt
      901 aacccgtccg gtcagccaat caatgccacg gtctttccaa ctttcgtcta aagttggagt
      961 atctaagcca gcgtccaagg taattccttc gtttggatcc cataaccttt tcccagtatt
     1021 gttaatctgg taaattttgg catcgccatt ggaaccttcc gtggtagctt ctgtagtaaa
     1081 agcggtcgag aaagcaacgc tattatctat tccgtaaact ttcgctgaat aacctttttt
     1141 tatcatctca tctcaccgcc ttaaacatgg ctcaattcgc cattgccggc aaaattaaac
     1201 gtcacctcga gaaccccgcc cactgttgcg ctgcgagaaa tagattcaac gatcgcattg
     1261 ccggaataat aatgggaatt gtcctcgtaa aaccgcatgg caacatcggc tccgatagtg
     1321 agctgtgatt gtcctgcgtc attcgggtct aatcttccga cgcagctccc cgaccaatct
     1381 ttctgagttc cggttttctt cttccattca tcgccaaacg ccgaggtgtc agcaatatcg
     1441 atattgacat ctaacgacca atcccccatc tcaccgatgg tggtggttcc tacttttata
     1501 tttccagcat agccttttaa tacagccatt tttattgcct cctatttata aaatcgaata
     1561 acaaacgaca caactgaacc aaaacgctct aaatcatcct catcgccgtt caggcgttca
     1621 atggtcaaat ccaatatctt ggcatagtat ttttttgccg tttctcctgt cccttcggta
     1681 acggttaact cgcttttaag tgctgcggct acctcttttg gaaggtctaa ggcggagtat
     1741 tcattagaaa ttggatacac ataaacccaa atttgatact catgctttcg ggtagcgtca
     1801 gaaaaagaaa ttactgcttc gctgtttatt gtgcttcgta ccacggcaaa gggtcgggtt
     1861 aaagctgaaa ggtctaaatt agggttataa agataagcgc tatcggctaa agcggtaaca
     1921 gtctcaacct gatcggctat tttctttaaa agtgaggata gcttcatagc ttctttaact
     1981 ccttgatggc ttctttgatg tcacgctgat atttgtctct ttgttcaaag agagaattgg
     2041 ttaaatagcc aacagtttgg gggtttttct tattgatgta attgcggatt ttggcatagg
     2101 ggacgttggg atggtatgat ccaacccccc aaaccatagg gagtttttgc tcggtagcta
     2161 aagaacctct caaagttccg gttaatactg gagctctttt tttggcattc tctcggattt
     2221 cttcagcgtt ttttttggtg acgaaatcaa cggtttgaac caccattcca gcccttttcc
     2281 cgaaagagtc aataaccttg tcaattcctt ctaacctcaa agttacatta agcacgtttt
     2341 ccacatccaa ctgctaagta ggcttcaatc tgatcaggcc taacaaactt cacttcatag
     2401 tctttgccgg aatgggtgat aatatctcca acctttactt gattggtaat taaggttcta
     2461 actatcagat caccccgttt taaaattcca gcttcaactg caaccctctc atctccggta
     2521 atttcctgaa catcagcggc aatactttta gaactttcgg taaagccgtc tccgccgcat
     2581 tcttcgcatt cgggatcggc aattccatat tcatctacac attcgcacgc tgtaagggtt
     2641 ttccagctta ccgaattgag agcagtgaat atctcaccag cagtggtttt aaaagcagtc
     2701 ttttcggctt cagtaattag tgtcatcttc ggtatcctca tcggaatagt cagttgtgtc
     2761 taaaccggat aaacgatcta agtcttggcc ccacaattca tcttgagatg agggagcact
     2821 ggaggctttt tcttcgtatt tttcagccag cttcatatag ttatcaataa tttttgattt
     2881 gtcgatctta acttcgccgc tccagaaaac aaacgctccc gataaggctt gggaacacaa
     2941 accacgaata acttcagagg ccgcctgata aacgctgtcg ttattttccg aaagtattgc
     3001 ggttaattcg tcatcggtat agccgctgct acttggtatt aactttttta gtctttcgag
     3061 gttggtcatt tttttcctct tttttcaatt ctttgaggat ttcttctaac aaatggaaga
     3121 tatttaaaag tagaatttca gtagtggttc ctgcgtgaat atcaagaat
//
LOCUS       DAOSOL010000167         3155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_30769_length_3155_cov_3.073226, whole genome shotgun sequence.
ACCESSION   DAOSOL010000167 DAOSOL010000000
VERSION     DAOSOL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30769_length_3155_cov_3.073226"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PK171_04735"
     CDS             <1..620
                     /locus_tag="PK171_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT05526.1"
                     /translation="REGITRILTDDIEYARELGYTIKLLAIARRIGEDIELRVQPVLL
                     PLSHPLSSVFGVENAIYVRSRTRDLTFRGPGAGGDATGSAMVGDIIDAIRNIKYAARG
                     RVGCTCMRDLTVLPMESLLSQNCVRVLALDVPGVLGKIASEFGNSGVSLSAVKQPIST
                     PGKLTNIYFLTHRIQERNIQNAIKSIQKLDVVKEVYAIRVEDGES"
     gene            1143..1454
                     /locus_tag="PK171_04740"
     CDS             1143..1454
                     /locus_tag="PK171_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05527.1"
                     /translation="MEPEEMKTILHCVRCQKDCEHVVIYHRGMISSIVCQKCGIEIGF
                     DQKKLMACYGEDLVNRVLTKPRRITEEYEKDLIKFMKDIPFRIITKPYRLIKEIDPFK
                     N"
     gene            complement(2143..>3155)
                     /locus_tag="PK171_04745"
     CDS             complement(2143..>3155)
                     /locus_tag="PK171_04745"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOT05528.1"
                     /translation="KRNRKGPLGRMSPLLISLQQLSKQFYLYDQTTNEKGVVDAVNAV
                     GLDILSGETLGLVGESGCGKSTLGRCILRLIEPTAGKIIYENQNLLKIPEKNFKPYRK
                     KMQIVFQDPFASLNPRKSIRFILEEPLIIHGMSNSQDRKKRLDETIEKVGLTQEDLKR
                     YPHEFSGGQRQRIGIARALILKPDFIVCDEPVSALDVSIQAQILNLLIDLQQEMKLTY
                     LFISHDLSVVKHISNRIAVMYLGRIVETAPTDEIFENPLHPYTMALLSSIPVPDPTLK
                     KDRLILEGDPPSPLNPPTGCRFHTRCPQQMDICSKEIPDRKAITSEHFVYCHLYNDLG
                     DS"
BASE COUNT          945 a          616 c          642 g          952 t
ORIGIN      
        1 atcgggaagg aattacccgt attttaactg atgatatcga atacgcccga gaattaggat
       61 acacaattaa gctattggca attgcccgaa gaattggaga agatatagaa cttcgagttc
      121 aaccagttct tctaccgctt tctcatcctc tctcatcggt gtttggagtt gaaaacgcta
      181 tatatgtccg ttcccggacc cgcgatttga catttcgggg tcctggtgct ggtggtgatg
      241 caaccggaag tgccatggtt ggagatatta tagatgccat ccgaaatatt aagtatgcag
      301 ccagaggacg ggtagggtgt acttgtatga gagacttaac tgttcttcct atggagagct
      361 tactatccca aaattgtgtt cgagttttag ccttagacgt tccaggagtg ttgggtaaaa
      421 tcgccagcga gtttggtaat tcaggggtca gtttgtcagc tgtcaaacaa ccgattagca
      481 ctccaggtaa gcttactaat atttattttt taacccatcg aattcaagag agaaatatac
      541 agaatgccat taaaagtatc caaaaactcg atgtggtcaa agaagtatat gccatccgag
      601 tggaagatgg tgaatcctaa aaaaagtgtt ttcttaaaat atttattgtc atgagcgtag
      661 tgatagtttg caatccgagg gttcaggatg atgatttttt ttaaaattct tccataaagg
      721 cttgatttat catgcccatt tcgtcattct gaggagtccg ttgtcttcta ccggacgatg
      781 taagaatctt atccaccttc tccgtcattc tgaggagcgt attatgcgat gtaagaatct
      841 catcattgaa aatatttata aagaaagaaa caaaaaggtg agattctcac ggattcgaaa
      901 agcgaagctt cgggatggtg cattgctaaa attacctctt gccaagattt ttaactcctt
      961 ctcccttgat gggagaaggt taggatgagg atgaaaaccc aagattcgac atgccatggc
     1021 aggtcgctac attaaacggg cacaattcat tgtgccccta cggataagat attcatttgt
     1081 aggggcttgg caagccaaac cctcagatta ttaagatttc accaaagaaa ggaggtaata
     1141 aaatggaacc agaagaaatg aaaactatct tgcattgtgt tcgctgtcaa aaagactgtg
     1201 agcatgttgt catttaccat agaggaatga tttcatcaat tgtttgtcaa aagtgcggca
     1261 ttgaaatcgg atttgaccaa aaaaagctta tggcttgcta tggagaagac ttggtcaacc
     1321 gggtactaac caaaccacgc cgaattactg aagaatatga aaaagacctt ataaagttta
     1381 tgaaagacat tccctttcgt atcataacca aaccttatcg tttgattaag gaaattgatc
     1441 cttttaagaa ttgaaaaatt cggttctctc cgcacctcaa tattgacttg taaaccacta
     1501 taccgctttg tattaaaaaa tgattcgggg gaaacacata atttaagccg ttattatgaa
     1561 gagagtagtg atgtcttgca atctcttcaa accctctcga tcattctgaa cggcatatta
     1621 agccgtgtaa gaatcttatc cactctttcc gtcattcaga ggagtccggt gtcttttacc
     1681 ggacgacgtg agaatcccat ccttttgata tgccataata aattggtaat cttagatttt
     1741 ttaacagtat tttaatgtgg tttgctttat caaaccactc taaatccgtc attgcgagga
     1801 gcgtcctgtg cgacgtggca atctcattta gcaattcttt cattcagagg agtccggtgt
     1861 tttaaccgga cattataaga atctcatctt tttcaattcc ttagatctac aatttctcaa
     1921 tactaccaat aataaaagtt gactcatgag attgccacga cctcaaaaaa ccaggtctcg
     1981 caatgacgga ttgggttgat gagatcctta cggcttcgaa aaacgaagcc tcaggatgac
     2041 taagaaagtt tgtgagattg ccacgacctc aaaaaaccag gtctcgcaat gacgagccag
     2101 agttccttct ccctctaatc ctctcccatt tcatgatctt tattacgaat cacccaagtc
     2161 attatataaa tgacagtaga caaaatgctc agaggtaata gctttcctat ccggtatttc
     2221 cttagaacaa atatccattt gttgaggaca acgagtatgg aatcgacatc cagttggtgg
     2281 atttagagga ctgggaggat ctccttcaag aattaagcga tcctttttca gggtaggatc
     2341 ggggacgggt attgacgaaa gaagtgccat agtataagga tggagtgggt tttcgaatat
     2401 ttcatctgtt ggagctgttt caacaattct tcctaaatac atgacggcta ttcggttact
     2461 aatatgcttt accacactca ggtcatgaga gataaagaga tatgtgagct tcatttcctg
     2521 ttgaagatca atgagtaaat ttaaaatttg agcctgaata gaaacgtcaa gagccgaaac
     2581 cggttcgtca cacacgataa aatctggttt taaaatcaat gctcgggcta ttcctattct
     2641 ttgacgttgt cctccggaaa attcatgagg atatcgcttg aggtcctcct gagttaagcc
     2701 caccttttct atagtttcat ctaaacgttt tttccgatct tgagaattgg acattccatg
     2761 gataatcaaa ggctcttcaa gaataaaccg aatcgactta cgaggattca aagacgcaaa
     2821 gggatcttga aagacaattt gcatcttttt acgataaggc ttaaaattct tttctgggat
     2881 ttttaataga ttttggttct catagataat tttccccgca gttggctcaa ttaagcgaag
     2941 aatacatcgt cctaatgtac tttttccaca accagactct ccaaccaatc caagggtttc
     3001 tccgctcaat atgtcgaggc caacagcatt gaccgcatca accactccct tttcattggt
     3061 ggtttggtca tataagtaaa attgtttgct tagctgttgc agggatatca ataaaggtga
     3121 catgcgaccc agtggtcctt ttcgatttct tttaa
//
LOCUS       DAOSOL010000168         3143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_30918_length_3143_cov_3.162889, whole genome shotgun sequence.
ACCESSION   DAOSOL010000168 DAOSOL010000000
VERSION     DAOSOL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30918_length_3143_cov_3.162889"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..816
                     /locus_tag="PK171_04750"
     CDS             325..816
                     /locus_tag="PK171_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOT05529.1"
                     /translation="MMSKKLEDAINEQIKNEFFSGYLYLSMAAQAEAMNLPGVAHWFK
                     VQAKEEQEHGMKFYDFINDRGGRVLLKAIDQPETEFKNVLDMFEKTLEHEKLVTSMIN
                     NLYEIALKEKDYPAQIMLQWFIDEQVEEEKNASDIIATLKMIGEKGHGLHMLDRELGK
                     REE"
     gene            881..1273
                     /locus_tag="PK171_04755"
     CDS             881..1273
                     /locus_tag="PK171_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT05530.1"
                     /translation="MPERSQDWYKQALRDLEQAVDSQKSGRHEWACFAAQQAAEKVVK
                     ALHLKNRQEAWGHVVSRLLHELPNSIEIPELLIEKARVLDNYYIPARYPNGHPESAHF
                     EHYDVLQSSEAIQYASEIIEFIGHQIAR"
     gene            1629..2612
                     /locus_tag="PK171_04760"
     CDS             1629..2612
                     /locus_tag="PK171_04760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT05531.1"
                     /translation="MNYRIFGKCGWAVSEIGFGAWAIGGSWGKVQEDDAVAALHRAID
                     CGLNFIDTADVYGDGRSEKIVAKVLKDRKERIYVATKAGRRLSPHVADGYNRENLNAF
                     VDRSLKNLEVETIDLLQLHCPPTEVYYRPEVFEVLDDIVKQGKLQNYGVSVEKVEEGL
                     KAIGYPGVKSVQIIFNIFRQRPTELFFAECRKRRVAVIARVPLASGLLTGKMTKETTF
                     SSDDHRNYNRYGQAFDRGETFAGVDYEKGLQAVEELRNLIPEGFNLTQFALKWILMFP
                     EVSVVIPGAKNPLQAEDNAKASSLPPLGEKVMEEIQKIYEASIKQDVHHRW"
     gene            2664..>3143
                     /locus_tag="PK171_04765"
     CDS             2664..>3143
                     /locus_tag="PK171_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT05532.1"
                     /translation="MARPVRIEFPGALYHITARGNKKENIFNHDRDRMVFLKILNETI
                     IRYQFICYAYCLMPNHYHLLIETPNGNLSLGIRELNGVYAQKLNWAYKTVGHVFQGRF
                     KAILVEKESYLLELCRYIVLNPVRAGMVQYPWDWQWSSYQATAGQAEKLKFLSTDWIL
                     "
BASE COUNT         1021 a          567 c          704 g          851 t
ORIGIN      
        1 gttatctatc cccattacta ccgtaaaata taatcataag tgggggtaaa taattttcaa
       61 aataattatg acacagtctg attgcgagga gcgcagcgac gtggcaatct cacccaccag
      121 ccccgtcatc ctgaggagcg ttttgtgcga cgtgagaatc tcatccaccc gccccgtcat
      181 tgcgaggagc gcagcgacgt ggcaatctca ttttttaaag tttttatgga ttaaaaaaga
      241 aaatttttta atccaagcat tgatttatca aacccacaat gggtttataa gatagaataa
      301 acacaaaaaa ggagagatgc tactatgatg agtaaaaagc ttgaagatgc aattaacgaa
      361 caaattaaaa atgaattttt ttccggttat ttatatcttt caatggcagc tcaagctgaa
      421 gcaatgaatc tacctggagt agctcattgg ttcaaggtac aagccaaaga agagcaagaa
      481 cacggaatga aattttatga ttttatcaat gatcgaggcg gccgagttct tttaaaggca
      541 atagatcagc cagagaccga atttaaaaat gttcttgata tgtttgaaaa aactttagaa
      601 catgaaaaac tggtgacctc gatgattaat aacctctatg aaattgcttt aaaagaaaaa
      661 gattatccag cgcaaatcat gttgcagtgg ttcatagatg aacaggttga agaagagaaa
      721 aacgcctcag atattattgc aaccctcaag atgattggcg aaaaaggcca tggtctacat
      781 atgttggatc gtgaactggg gaaaagagaa gaataattaa ttagcaggag aatgggtagt
      841 tttaaactac ccattcttca tttttttttg gagggaaaga atgccagagc gttcccagga
      901 ttggtataag caggctcttc gagatttaga acaagccgtt gattctcaaa agtcaggccg
      961 ccatgaatgg gcgtgttttg cagcccaaca agccgctgaa aaagtagtaa aagctcttca
     1021 tttaaaaaac cgtcaagaag cctggggaca tgttgtctct cgccttcttc acgagcttcc
     1081 taattcaata gaaattccag agctgcttat tgagaaagct cgagttcttg ataattatta
     1141 tatccccgct cgttatccca atggccatcc tgagagtgct cattttgagc attatgatgt
     1201 tttgcaaagc tcggaggcaa ttcaatatgc cagtgagatc attgaattca tcggtcatca
     1261 aatagccaga tgaaaaacag gttatttcat ccctaaaaaa atggacaaat acaattatca
     1321 aaatgaacca aaatgtcgtt caaattggat attttggtct ctatgctcga ggtgattggg
     1381 aagtgggaag tgatttaaga cctcatcatt atattatccc aatccgatct tccttttgaa
     1441 aaaagaaatc aagattggca agagcatcaa ttaccggtac caactgatat tctcatttat
     1501 acccagcagg aatgggaagt aatgagaaaa gaagaattgc ctttttttaa aagaataaac
     1561 cgtgaaatta aatggattta ttaggaaaat acaaattcaa aaaaactatc ataagtaagg
     1621 agaaataaat gaattaccga atttttggga aatgtggatg ggcagtaagt gaaattggtt
     1681 ttggtgcttg ggcaatcgga ggaagctggg gaaaggtaca ggaagacgat gccgttgcag
     1741 ccctccatcg agcgattgat tgtggtttaa atttcattga tacggctgat gtttatggtg
     1801 atggtcgaag tgaaaagata gttgctaagg ttctcaaaga ccgaaaagag agaatctatg
     1861 tcgcaacgaa agcggggaga cgtctctcgc cccatgtagc tgatggatat aaccgggaaa
     1921 atttaaatgc ctttgtagac cgaagtttaa aaaatttaga ggttgaaacc atcgatcttt
     1981 tacaacttca ttgtcccccc accgaggtct attatcgacc ggaggtattc gaagtattag
     2041 atgacatagt gaagcaaggg aaacttcaga attacggagt aagcgttgaa aaggttgaag
     2101 agggcttgaa agcaattgga taccctggcg tgaagagtgt ccagattata tttaatattt
     2161 tccggcagcg accaacagag ctgttttttg ccgaatgtcg aaaaagaaga gtagcggtta
     2221 tagcccgggt tccactggct agtggtctcc tcaccggaaa aatgaccaaa gaaaccacct
     2281 tttcttccga tgatcaccgc aactacaatc gatatggtca ggcttttgac cggggcgaaa
     2341 cctttgccgg ggtagattat gaaaaagggt tacaagcggt ggaagaattg cggaatctga
     2401 tccctgaagg atttaacctg acacagtttg ctctgaaatg gattctcatg tttccagaag
     2461 tgagcgttgt gatccccggt gccaaaaatc ccctgcaggc tgaagacaac gccaaagctt
     2521 ccagccttcc accccttgga gaaaaagtta tggaagaaat ccaaaagatc tatgaagcct
     2581 ctataaagca ggatgttcac caccgctggt aaaacttggg gtcaggtctt gattcttgaa
     2641 tttttataga aggattattc tatatggctc gaccagttag aattgaattc cctggtgctc
     2701 tgtatcatat caccgccagg gggaataaaa aagagaatat atttaaccat gaccgagacc
     2761 gaatggtctt tttaaagatc ctgaatgaga cgataatcag gtatcagttc atttgttatg
     2821 cctattgtct catgccgaac cattaccatc tactgataga aactcccaat gggaatctat
     2881 ccctggggat tagggaatta aacggagtgt atgcccaaaa attgaattgg gcttataaga
     2941 cagtaggcca tgtattccaa gggagattta aggcgatttt agttgagaag gaaagctatt
     3001 tattagagtt gtgtaggtat attgttttaa atcccgtccg agcaggtatg gtgcaatatc
     3061 cctgggattg gcagtggagt agctatcagg caacggctgg acaagccgaa aaactcaaat
     3121 ttttatcaac cgactggatc ctt
//
LOCUS       DAOSOL010000169         3135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_31047_length_3135_cov_3.211039, whole genome shotgun sequence.
ACCESSION   DAOSOL010000169 DAOSOL010000000
VERSION     DAOSOL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31047_length_3135_cov_3.211039"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PK171_04770"
     CDS             complement(<1..658)
                     /locus_tag="PK171_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HOT05533.1"
                     /translation="MTGEVTIDIPKAFQFLLEPHRYKSAYGGRGAGRSWSFARVLAVL
                     ASYQKKRILCTREYQNSIKDSVHKTLSDQIELLNLTPYYNITKTEIISNVGSEFIFKG
                     LQHPLEIKSIEGIDIVWLEEAQSVSEESWRFLIPTIRKENSEIWLSWNTGAKTDPTYQ
                     RFVINKPDDCVSKLLTYKDNPFFPNTLRKEMVHCKRVDKDAYNHIWEGLPQHLSDALV
                     F"
     gene            complement(655..1182)
                     /locus_tag="PK171_04775"
     CDS             complement(655..1182)
                     /locus_tag="PK171_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05534.1"
                     /translation="MVKKKRIKDQPKNKVGRPSSYTDKVGKYICQEIMKGRTITSICK
                     EEGMPCLPTVFNWLNKLNNAYNEDFLKSYSQAREIQAEVMADEIKDIADNGENDTYET
                     INPKTGKMETKTDFDHIKRSALRVESRKWLAAHLLPRKYSDRVQLTGAEGKDLIPAVP
                     TKVVFNFVGEEEEKE"
     gene            1982..2593
                     /locus_tag="PK171_04780"
     CDS             1982..2593
                     /locus_tag="PK171_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05535.1"
                     /translation="MKDNKITLRQFIEKFDNGEFDSKSTDIQCDAGWYDWFCKDSSLR
                     NKTYSLTKKIKQLLPSSKINQDTMYVFFKNNCPCVGKLYDDFRFCDMETGEVIYTICP
                     AVGYTKTFGRSEVWGKENDFKEAIVAGTWNDVLFFFLGVDKKKEREEKRIAVEERKKE
                     KDLLKDEWAVREYLINQIEDKTVSSDNWYEFTTVKLIKISLAL"
     gene            2700..>3135
                     /locus_tag="PK171_04785"
     CDS             2700..>3135
                     /locus_tag="PK171_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05536.1"
                     /translation="MKKLSEIALLADLTISAWSGSIADKKTTDEILDKKNARRDRGRF
                     VKNLLMDTKSLKTITNKMYHEYKMKSTAWEDGRRLLPVEFFDSITKLHRDCSDELEKA
                     LDEFGKSYPEYKEKAKEELGDLYDETEFPSFDEFKRKWKIRLD"
BASE COUNT          974 a          603 c          604 g          954 t
ORIGIN      
        1 tgaataccag tgcatcactt aaatgttgcg gtaatccttc ccagatatga ttgtacgcat
       61 ctttatctac ccgtttgcaa tgcaccattt ccttacgcaa agtattaggg aagaacggat
      121 tgtctttata ggtaagaagt ttggatacac aatcatcagg tttgttgatt acaaatcttt
      181 ggtaagtagg atcagtcttg gccccagtat tccaacttaa ccatatctcg gaattttctt
      241 tccggatagt cggtatcaag aatcgccaac tttcttctga tacgctttga gcttcttcca
      301 gccagacaat atctattcct tcaatcgact ttatttccaa tggatgttgt agccccttga
      361 agataaattc agaaccgaca ttgctgataa tttctgtctt ggtgatgtta tagtatggag
      421 taagatttaa aagttctatt tgatcagata gggttttatg aactgaatct ttaatactgt
      481 tttgatattc tctggtgcat aggattcttt tcttttgata agatgcaagc accgccaata
      541 ccctcgcaaa tgaccatgac cgtcccgcac ctcttccacc ataagcggat ttataccggt
      601 gtggttccaa cagaaattga aatgctttgg gaatatctat agtgacttca ccggtcattc
      661 tttttcttcc tcttctccta cgaaattaaa taccaccttt gtgggcactg ccggtattaa
      721 atcttttcct tctgctccgg tcaactgaac tctatccgaa tactttctgg gcaataaatg
      781 cgctgctaac catttcctgc tttccactct taatgcactt cttttgatat gatcgaaatc
      841 tgtttttgtt tccattttcc cagtctttgg gtttatggtt tcataagtat cattctctcc
      901 gttatctgca atatctttta tttcatcggc catgacttcc gcttggattt ctctggcttg
      961 tgaataggac tttaaaaaat cttcattgta agcgttgttt aatttattta accagttaaa
     1021 aacggttggt aaacacggca tcccttcttc tttacaaatc gaagtaattg ttctgccctt
     1081 catgatttct tgacagatgt attttcctac cttgtcggta taagagcttg gtctgccgac
     1141 tttgtttttt ggttgatctt tgattctttt ctttttgacc atgacccttt tccccttttg
     1201 aaaaaaacat ccctatatat agggtgattt taaagcgatt ttcttgatac tttttgcatt
     1261 attttttaaa aataccaatg attttgatat gttagggatg aaaatatttt taccatacca
     1321 tgaaattact ttaaaaaaac cgcaataatc tcttaactca ttgattttat tatactatac
     1381 cgggaatata tagcatatat actgctaacc tattgatttt attattaaaa aaaaatcaat
     1441 atttttatag aaacttattg atttttcccc tgaactatac tataaataat caacatatat
     1501 gatcttttac aattaaatat cggttgaggt tgtcagggcg gtgtccgagc caagaccgaa
     1561 agcgaaccca cgcagtgctt gaatggacgg aatcaatgtc gggcgcgagt agtaaatgcc
     1621 ccacccggat cagaatcgaa accaaccaac atccataacg cgtgaaacag gaaactgctt
     1681 gaaagtccga aaaaggtcag caactggtag cgagccaaac ccaaagaaat tctggagggg
     1741 gatctaaggc ggacgtaaag tagtccagcg agagacggcc cgatatttag atggcaagcc
     1801 acagtatcgg ttaatcatga tgatgctcat gatgtcattc aaggcaaggc atgaaccctt
     1861 gaagccataa aggtaaggct tgaatccctt cgttatgatt tcttatgcgg ctcatctatt
     1921 ctttctggta ggtgagtcga aacgaaacca taacccttaa aaaaaagaaa ggagcaacaa
     1981 aatgaaagac aacaagatta cattaagaca gttcattgaa aaatttgaca acggggaatt
     2041 tgattccaaa tcaacagata ttcaatgcga tgcaggatgg tatgattggt tttgcaaaga
     2101 ttcaagcttg aggaacaaaa cctattcgct gacaaaaaaa atcaaacagc ttctgcccag
     2161 cagtaagatc aatcaagata cgatgtatgt tttctttaaa aacaattgcc cttgcgttgg
     2221 caaactttat gatgactttc gtttctgcga catggaaacg ggcgaggtca tttacacaat
     2281 ctgccctgcg gtaggatata ctaaaacttt cggtcgatca gaagtatggg gcaaagaaaa
     2341 tgattttaaa gaagccattg ttgcgggaac ttggaacgat gtgcttttct tttttctcgg
     2401 agttgataag aaaaaagaaa gagaggaaaa aagaattgct gtcgaagaga ggaaaaaaga
     2461 aaaagacctt ttgaaagatg aatgggcagt cagagaatat ttgatcaacc aaattgaaga
     2521 caaaacagtt tcttctgaca actggtatga atttacaaca gtaaagttga ttaaaatttc
     2581 attggctctt taatgccgaa atcggagcga tgagctccga tacactggtt ggtggttacc
     2641 ccagtgctga tgaggcaaac caaataaatt ataacaccat aaatgaaagg agttccaaaa
     2701 tgaaaaagtt atccgaaatc gcattgctgg cagacctgac catttctgct tggtcgggtt
     2761 caatcgcaga caaaaaaaca accgatgaga tccttgacaa gaaaaacgcg agaagagatc
     2821 gtggccgttt cgtaaaaaat cttcttatgg acacaaagtc tttgaagacg attacaaaca
     2881 aaatgtacca cgaatataag atgaagagca cggcatggga agatggtcgg cgactgctcc
     2941 cggttgagtt ctttgattca attaccaaac ttcatcgtga ttgttcggac gaacttgaaa
     3001 aagcccttga tgaatttgga aagagctacc ccgagtacaa agaaaaagcc aaagaagaac
     3061 tcggcgacct ttatgacgag accgagtttc cctctttcga cgagttcaaa agaaagtgga
     3121 aaattcgtct tgatt
//
LOCUS       DAOSOL010000170         3129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_31134_length_3129_cov_3.334092, whole genome shotgun sequence.
ACCESSION   DAOSOL010000170 DAOSOL010000000
VERSION     DAOSOL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31134_length_3129_cov_3.334092"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            422..1045
                     /locus_tag="PK171_04790"
     CDS             422..1045
                     /locus_tag="PK171_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HOT05537.1"
                     /translation="MSQDKFELSSERIQSLTDGIFAFAMTLLVINIDLPSTPHKIDPE
                     LLGKYLFSLWPDFFHYVLSFILLSLFWVEHHQQFYYIKKINRKALWINILNLLFVALF
                     PFSTSLMGDYSSQSIAAAFFISNLLVIKIFLYLNWVNAISNKELIVSDINEEIIFEKK
                     SHHLFFIITSLIGLVLAFIIPEWSTLPFVLTFFHRSILKNKKKLYNK"
     gene            complement(1049..1912)
                     /locus_tag="PK171_04795"
     CDS             complement(1049..1912)
                     /locus_tag="PK171_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05538.1"
                     /translation="MSRYRNIRLLSSTAQYLIIVILLVAVLFPIAWMVSTSFKNHHDL
                     FETPPRWIPRNPTAAAYQRIFISRAGAGGVNFLTYFKNSLIVCGATVIFCILLAIFSG
                     YALSRFHRRGRKSIFTSILITQMFPLPMFLITFYIMFMRLKLLNSYTGLIIAYTSFSL
                     PFCIWMIKGFFDKIPLELEEAALIDGCSRMSALWRVVIPLVSPGVVAIGIFAFLSAWD
                     EFMFSLTLMSQDAMRTLPPGIVLSFVGEFDVRWEDMMAASVVATLPVLIVFLILQKYL
                     VEGLTAGAVKG"
     gene            complement(1914..2810)
                     /locus_tag="PK171_04800"
     CDS             complement(1914..2810)
                     /locus_tag="PK171_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05539.1"
                     /translation="MSIGKKRSDKRLYFLLILPSATILILTIGYPLINGIILGFHYYR
                     LTDPARGVVFNGLQNFRMLLFGNRIFWISLYRTFLYCLGSTGVSFLFGFLIALLLYGP
                     IRNRSLFRSLFLLPWVIPSTVVALLWLWIFNPQYGILNFLLKDLGLIRQFQSWLVNPS
                     LALPSVILATSWKYFPFFMLMLLAGLETIPKELFEASTIDGATFFQRLRYIILPSLKE
                     IIIIVTILEIIWEFQNFTIIWILTKGGPVYQTTTMGIQIYRTAFRNYDMGMGAAMGSL
                     SLVAMLIFSLSYIKTLKFGEGD"
     gene            complement(2833..>3129)
                     /locus_tag="PK171_04805"
     CDS             complement(2833..>3129)
                     /locus_tag="PK171_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05540.1"
                     /translation="YMEFLISDPEITKTYYQDTGLMPTRISLLNDPIYKDDPFVRQVL
                     NQTEYMKKPLGWGPRWGRVGEIVMAAVQEVVLMGRDVQSALDEAVRQIDIELSM"
BASE COUNT         1046 a          597 c          614 g          872 t
ORIGIN      
        1 tcaaggtaac aatgataata aaaggaattt attggcacaa aactgctcat tctgaatcac
       61 aactgaaggg tcgtgagcag ttttgtgaaa ttaataaaga atttattaat ggtttttacc
      121 ttttttaaaa ttaaaaaatt gattcgggtt ttgtaattat caggagaggc cttggtacca
      181 catgttggta ccaggtgaaa atgaaaatac ggttttattg gcctgtcatt cttaggaacg
      241 tatttttcga cgtgagaatc ttatctattc caattccaca acttttaaaa gatgagattc
      301 tcaaggggga agtcactgct caagatgatg ttgtttttaa ctcaatgcta ttttttcaaa
      361 agaaaagatg ttttaagtta atctaaatag aaaccaaccc ttctaaggaa gagaatttaa
      421 aatgagtcaa gataaatttg aattatctag cgaacgtatt caatcactca ccgatggaat
      481 atttgccttt gcaatgacac tattggttat taatatagat ctccccagta cgccccataa
      541 aatagatccc gaacttctcg ggaaatacct cttcagttta tggccagact tttttcatta
      601 tgttttaagt tttattctat tatcactttt ttgggttgaa caccatcaac agttttatta
      661 cataaagaaa atcaatcgta aggcactttg gattaatata ttaaatctct tatttgttgc
      721 tttgtttcca ttttctacat cacttatggg agactactcg agccaatcaa ttgcagctgc
      781 tttttttata tccaacttgc tggtgatcaa aatatttctt tatctaaact gggttaacgc
      841 tatttctaat aaagaattaa ttgtttctga tataaacgaa gaaatcattt ttgaaaaaaa
      901 atcacatcat ctttttttca ttatcacgtc actgataggt ttagttcttg cctttataat
      961 tccggaatgg agcacccttc cttttgttct tacttttttt caccgttcaa tcctaaaaaa
     1021 taagaaaaaa ctttataata aataaaaact atcccttaac ggcaccggcg gttaatcctt
     1081 caaccaggta tttttgaaga attaaaaaaa ctattaaaac cggaagggtg gcaacgacac
     1141 tggcagccat catatcctcc cagcgaacgt caaattcgcc tacaaaagag agaactattc
     1201 cgggaggaag agtccgcata gcatcttgag acatgagagt gagggaaaac ataaactcat
     1261 cccaggcaga gagaaatgca aaaatgccaa tggcaacaac tccaggagat actaaaggaa
     1321 tgaccacccg ccaaagagca ctcatccgac tacagccatc gatcaaggca gcttcttcga
     1381 gttctaaggg aattttatca aagaatcctt ttatcatcca gatgcaaaaa ggtaatgaga
     1441 agctggtgta ggcaattatc aaaccagtat aagaatttaa aagtttcaat cgcataaaca
     1501 tgatataaaa ggtaattaaa aacattggga gaggaaacat ctgagtgata aggatactgg
     1561 taaaaatact tttccttcct cgccgatgga aacgagaaag tgcatatccg gaaaaaattg
     1621 ctaataggat acagaatatt acagtagcac cgcaaactat gaggctgttt ttaaaataag
     1681 ttaggaaatt tacccctcca gctccagctc gagaaataaa aatgcgttgg taggcagcag
     1741 cggttgggtt gcgaggaatc cagcggggtg gtgtttcaaa aagatcgtga tggtttttga
     1801 aagaagtcga taccatccag gctattggaa acaatacggc tacgagtaaa atgacgatga
     1861 ttagatactg agcggtactc gacaggaggc ggatgtttcg gtaacgtgac atatcaatca
     1921 ccttccccga attttaaggt tttaatatag gataaactga aaatcaacat agctactaga
     1981 gataaagaac ccatagctgc tcccatcccc atatcataat tccgaaaagc agttcggtag
     2041 atctgaatcc ccatggtcgt tgtctgatac actgggcctc ccttggtaag aatccagatg
     2101 atagtaaagt tttggaattc ccaaatgatt tccaggatgg ttacgatgat gattatttcc
     2161 ttgagacttg gcagaattat atatcgaagt cgctgaaaaa aagtcgcccc atcaatggta
     2221 gatgcttcaa agagttcctt gggaatagtt tcaaggccgg ctaaaagcat cagcataaag
     2281 aagggaaaat atttccaact cgtggccaga attaccgaag gtaacgctaa gctgggatta
     2341 accagccacg attggaattg tctgattaat cccagatctt taaggaggaa gtttaaaata
     2401 ccatactgag gattgaaaat ccaaagccat agcaaagcta caacagttga gggaatgacc
     2461 caagggagaa gaaacaggct gcgaaagaga ctccgatttc gaatcggacc atagagcagg
     2521 agagcaataa ggaaaccgaa taaaaaggaa accccagtag aaccaagaca atagagaaag
     2581 gttcgataaa gagatatcca gaatattcga tttccaaaca acagcatccg aaaattctgt
     2641 agaccattga aaaccacccc ccgtgctgga tcggtgagcc gataataatg gaaacccaga
     2701 ataatcccat taatcagagg atagccaatg gttaagatca aaatggttgc cgacggaagt
     2761 attaagagga aatagagcct tttatcgctt cgttttttcc caattgacat ttctgttctt
     2821 ctccctgatt atttacattg aaagctcaat atcgatctga cgaacagctt catccaaggc
     2881 gctttgtaca tcccgaccca tcaaaaccac ttcttgaacg gctgccatga cgatttctcc
     2941 aacccgaccc caacgaggtc cccaaccgag tggctttttc atgtactcag tctgattcag
     3001 tacctgccgg acgaaaggat cgtccttgta tatcggatcg ttgaggagtg aaatccgggt
     3061 aggcatgaga ccagtatctt ggtagtaggt tttagtgatt tcagggtcgc tgatgagaaa
     3121 ttccatgta
//
LOCUS       DAOSOL010000171         3126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_31166_length_3126_cov_3.871703, whole genome shotgun sequence.
ACCESSION   DAOSOL010000171 DAOSOL010000000
VERSION     DAOSOL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31166_length_3126_cov_3.871703"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="PK171_04810"
     CDS             complement(<1..1983)
                     /locus_tag="PK171_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05541.1"
                     /translation="MRRLVLVFFLLICLPFSVNAEEKSIELTGERLTYHGDGEEIIAI
                     NGKLIYQDVVLEGQKIHIFLQKKQLEAFGDVVVTENKEQFHASQANFNWDTDSWEFFD
                     ASSEFTGRSIVGKVFFRGKNLSREPDLTIVNEAYMTSCDLEKPHYHIDAQKIEIIPDK
                     RVILHGISYWDFGFKIFTLPYYVIFLDRKEQLPFIPIIGQSSSYGFYVNLFFNYFVNY
                     DSYGTVYLDWYQKDGWGIGVRHYIERQDPYEKGQLYLYYRDPSESQSTLKANLQYQKK
                     LDQYTDLTFNTDYNLLLDDQKDTISSQLAILHKKGRSTTRLNLTYNSNASNENSNLAS
                     YLNYTYDFGDKLIGTLILDYKRLSQWGNYIDEDLKYQASFQKSSGSMVYALRYDGHAD
                     PEGDLFTGDPGRYFFKIPEFEVAGSRTRIGKSDFYYKTSFVGGHYFEEETNVRDERYR
                     FQLQLDGTNQIGENTTLKPSINFIQDFYGNGFARYIWEGNILLTQLVSDQGKVSLNYN
                     RSGYDGATPFKTDYTTRETNYASLSASYKSNYWEIGIEAGYDFMNQDFTDTIFSLKYH
                     ENDEHSIALKGSYDFDTGDWLGLAVDTTWRINKDWKLSVDGTWNLTNGSLQGLEVGVT
                     RDLHCREITFYYDKPRDTFWLEYTIKAFPSQKVTLGG"
     gene            complement(1990..2685)
                     /locus_tag="PK171_04815"
     CDS             complement(1990..2685)
                     /locus_tag="PK171_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05542.1"
                     /translation="MKKHTVLVIFVAFFLLLVGQSLDAQEPTITPEENTPEKKEVLLE
                     SADKITYDSKGETFVAIGQVIAIQGKNRIQCQELNFNLKDNTGVFEGNVTVTRDQTEI
                     IATSMEGDFDEELYQFSGDVILKKEREEETGTSTIIWKAPTLTYNGETEEARSENGSD
                     IVWKETTIKTDRAVYFPKDDEKNQLERIEMEGEILITEKERELQVGKAVYYLDSETLE
                     AEKIIKAKFILGD"
BASE COUNT          942 a          619 c          578 g          987 t
ORIGIN      
        1 tcctcccaaa gtgacttttt gcgaaggaaa agccttaatg gtatattcaa gccaaaacgt
       61 atctcgaggt ttgtcatagt aaaaagtaat ttctcgacaa tggaggtcac gagtgacccc
      121 cacttctaag ccttggagac tgccgttggt aagattccag gttccatcaa cagacaactt
      181 ccaatcttta ttgattctcc aggttgtgtc aactgccaaa cccaaccaat caccagtatc
      241 aaaatcatag ctccctttta aggcgataga atgctcgtca ttttcatgat atttgaggct
      301 gaatatggta tcggtaaaat cttgattcat aaaatcataa ccagcttcaa ttccgatttc
      361 ccaataattg cttttgtagc tggcactaag gctagcataa ttggtttctc tagtggtgta
      421 atcggtttta aagggtgtcg ctccgtcata accactccgg ttataattaa gagaaacttt
      481 tccttggtca gaaaccaatt gagtcaacaa aatgtttcct tcccagatgt aacgagcgaa
      541 gccatttcca taaaaatcct gaataaaatt tatcgaaggt tttaaggtgg tattttctcc
      601 aatttggttg gttccatcta actgtagctg aaatcggtag cgctcatcac ggacattggt
      661 ttcttcttca aaatagtgtc ctccaacaaa actggtttta taataaaaat cagatttacc
      721 aattcgggtt cgacttccgg caacttcaaa ttcaggaatt ttaaaaaaat acctgccagg
      781 atcaccagtg aataaatcac cttctggatc ggcatgtcca tcataacgga gggcataaac
      841 catgcttccc gaggattttt gaaatgaagc ctgatattta aggtcttcgt caatataatt
      901 tccccattga gaaagccttt tataatccaa gatgagggtt ccaattaatt tatctccaaa
      961 atcataggtg taattgagat atgaagccaa attggaattt tcattgctgg cgtttgagtt
     1021 ataggtgagg ttaagccggg tggttgacct tccttttttg tggagtatgg ccaactgact
     1081 ggaaatagtg tctttttggt catcgaggag aagattataa tcggtgttga aagtcaggtc
     1141 ggtatattgg tcaagttttt tctggtattg aagattggct ttgagtgtac tctgggattc
     1201 acttggatct ctatagtaga gataaagctg gcctttttca tagggatcct gacgctcaat
     1261 gtagtgacga actccgattc cccaaccatc tttttggtac cagtcgagat atactgttcc
     1321 ataggaatca tagttaacga aataattgaa aaaaagattg acataaaaac catatgaaga
     1381 ggattgtcca ataataggga taaatgggag ttgttctttt ctatcgagaa atatcacata
     1441 gtacggaaga gtaaagattt taaatccaaa atcccaataa gatatcccgt gaagaataac
     1501 tctcttatct ggaataattt caattttttg agcatcgata tgataatggg gtttttctaa
     1561 atcacaagag gtcatgtatg cttcattgac aattgttaaa tctggttctc ggctgagatt
     1621 ttttcctcga aagaaaactt ttccaacgat tgaccttcca gtaaattctg atgaggcatc
     1681 gaagaattcc caactatcgg tatcccaatt gaaattagct tgactggcat gaaattgttc
     1741 tttattttcg gtgactacga catctccaaa agcctctaat tgctttttct gaaggaagat
     1801 gtggattttt tgaccttcca aaacaacatc ctgatagatc aattttccgt tgatggcgat
     1861 tatttcttct ccatcaccat gataagtcag gcgttcccca gtcagttcta tagacttttc
     1921 ttcggcatta accgaaaacg gaagacaaat taaaaggaag aaaaccaaga ccaaacgacg
     1981 catctgggat taatccccaa gtataaattt agccttaatg attttttctg cttctaaagt
     2041 ttcagaatca agataataga cagcttttcc aacctgaagt tctcgttctt tttcggttat
     2101 caatatttcc ccttccattt caattcgttc taattgattc ttctcatcat ccttagggaa
     2161 atagacagcc cggtcagttt ttattgtagt ttctttccaa actatatcag aaccattttc
     2221 gctcctagct tcttcggttt ctccattata agttaaagtc ggtgctttcc aaataatggt
     2281 tgatgtgcca gtttcttctt cccgttcttt ttttaaaatg acatcaccag aaaactgata
     2341 tagttcttca tcaaaatcgc cttccatcga cgtggcaata atctcggtct ggtcgcgggt
     2401 aacagtaaca tttccttcaa agactccagt attgtctttt aaattaaaat tcagctcctg
     2461 acattgaata cgattttttc cttgaatagc gataacctga ccaatggcta caaaagtttc
     2521 tcctttagaa tcataagtga ttttatcagc actttctaaa agaacctctt ttttttcagg
     2581 agtgttttcc tcgggtgtta tagttggttc ctgagcatca aggctctgac caactaataa
     2641 caaaaaaaat gcaacgaata tgacaagaac agtatgtttt ttcaagggta ttcccccctt
     2701 cggagcgaat taagtgtatt atatcattat aaagaaggtt cggttcattt tatctcaaag
     2761 gtcatgattc cgagttttaa ttaataaaaa attcgcttta atagtatgat gagattcagt
     2821 gatcaatact gagtggtgag aaaatctccc attatccaaa gggagaggga taataaagat
     2881 tgagctcatc cacccagtca gtcattctga gaagtccggt gttttctacc ggacgacgtg
     2941 agaatctcat tcttaaaagt atttatgaag aataaaaaaa cccaaaaaga tgagatcctc
     3001 acggcttcga aaaacgaagc ctcaggatga cgccggctgc gtcagatgag atcctcacgc
     3061 ggaaaagcac cgctcaggat gaccgattaa atgcacaccc ccctaacccc cctcaatggg
     3121 ggaatt
//
LOCUS       DAOSOL010000172         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_31271_length_3119_cov_3.491841, whole genome shotgun sequence.
ACCESSION   DAOSOL010000172 DAOSOL010000000
VERSION     DAOSOL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31271_length_3119_cov_3.491841"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1400)
                     /locus_tag="PK171_04820"
     CDS             complement(60..1400)
                     /locus_tag="PK171_04820"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT05543.1"
                     /translation="MMNEHILLVNPWIYDFTAYDLWMKPLGLLYLASHLKNSGYKVTL
                     LDCMDRHYPFMPLPDHPGPKRLKSVGCGSYYREKIPKPAQFLSIPRYWSRFGIPWDEV
                     KKQLKEIEPPGWVFITGTMTYWYPGQVAFLNLVRQLWGKTQVIIGGWYPTLCSEHAHQ
                     WGFDRIIEGIDPLLVIKQLSSDFKELSTITGYKEFFSVKPAFDLYKKLDYTVVLTSIG
                     CPFRCTYCASGRYFSEFWRRPWEQVLDEITYDYENYRIQDLAFYDDALLYRAEESFLP
                     LLKALQKERLPLRFHLPNSIHARYVTREIAELMRECNFETLRISLEFSDVDSQKNTGN
                     KVDNFIFEKAIQSFIQAGYAPEELEVYLLFGLPGLKAKDYELSIEYVTDLGLKPRLSL
                     FSPLPGTPDFEKVIMHQIKDDPLFQNKIAYLYLSEQNELYEALQRKISKYTFFH"
     gene            complement(1479..2801)
                     /locus_tag="PK171_04825"
     CDS             complement(1479..2801)
                     /locus_tag="PK171_04825"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOT05544.1"
                     /translation="MEEANRIYLSEREMPTIWYNIIPDLPRPLDPPLHPGTKEPVTPQ
                     DLEPIFPPSLIQQEVSSQRFIDIPGEVLDIYRLWRPSPLYRARRLEKALQTPARIYFK
                     YEGDSPVGSHKPNTAVAQAYYNKKDGTKRLTTETGAGQWGSALSMACHYFGMECTVYM
                     VNVSYRQKPYRRVLMQTWGANVFPSPSENTKTGSDILHETPDSIGSLAIAISEAIEDA
                     VNTPHSKYSLGSVLNHVLLHQTIIGEETKLQLEKVGEKYPDIVIGCVGGGSNFGGMAF
                     PFLRDKIAGTKNFRAIAVEPKSCSTLLTGKYEYDLGDVAGMTPYLKMYTLGHDFIPPG
                     IHAGGLRYHGMAPLVSLLYNAGIIEAQSYKQNEIFDSAVLFTKTEAILPAPESAHAIK
                     AAIEEAKKCKETGEDKVIVFNLSGNGYLDLGAYDIFVNGGFPEANNGS"
BASE COUNT          930 a          655 c          601 g          933 t
ORIGIN      
        1 gaagatattt catctaatta attaaattat taatatttta agttggttta tttgttgcgt
       61 taatggaaaa aagtatattt agatattttc ctttgcaacg cctcatataa ctcattttgt
      121 tcggataggt aaagataagc tattttattt tgaaacaagg ggtcatcttt gatttgatgc
      181 atgataacct tttcgaaatc tggtgtacca ggaagtggag aaaatagaga caaacgaggc
      241 tttaatccca aatcagtaac atattcaatc gacaattcat agtccttcgc ctttaaacca
      301 ggaaggccaa aaagaagata gacttccagt tcttcagggg cataaccggc ttgtataaaa
      361 gattgaatgg ctttttcaaa aatgaaatta tccaccttat ttccagtgtt tttttggcta
      421 tcgacatcgg aaaattctag gctaattcga agggtttcaa agttacattc tctcatgagt
      481 tcggcaattt cccgagttac ataccgggca tggatagaat tggggaggtg aaaacgaagg
      541 gggagtcgtt ctttttgtaa agcttttaga aggggtagaa aactttcttc ggctcgataa
      601 agcaatgcat catcataaaa agctaggtct tgaatccggt aattctcata atcataggta
      661 atttcatcga gtacctgctc ccagggccgc cgccaaaatt ctgagaaata cctcccggaa
      721 gcacaataag tacagcgaaa ggggcaaccg atagaagtca aaacgaccgt atagtcaagt
      781 tttttataaa gatcaaaagc tggcttcacc gaaaaaaact ctttataacc tgtaatagta
      841 gataattcct tgaaatctga actgagttgc ttgatgacca gcaaggggtc aatcccttca
      901 atgatccggt cgaatcccca ttggtgagca tgttctgaac agagagtagg ataccaacca
      961 ccaataatta cttgagtttt tccccataat tgacgaacaa gatttaaaaa agcgacttga
     1021 ccaggatacc aataagtcat ggtaccagtg ataaacaccc atcctggggg ttcaatctcc
     1081 ttgagttgtt tcttaacttc atcccaaggt attccaaaac gtgaccaata acgaggaatc
     1141 gacaaaaatt gagccggttt aggaatcttt tcccgataat aactaccaca acccacactt
     1201 ttaagccgtt tcggccccgg atggtctggt agaggcataa aagggtaatg gcgatccata
     1261 caatctagga gggtaacctt ataaccagaa ttcttcaagt gagatgccag ataaagtagt
     1321 cccaatggct tcatccagag gtcataagcg gtaaaatcat aaatccaagg attgactaaa
     1381 agaatatgtt cattcatcat tgatactcca cttcaaatac caggcaaaaa aaaatgcctc
     1441 ctgtagtttc ccactggagg catttttttt caattcactt atgaaccatt attggcttca
     1501 gggaaacctc cattaacaaa aatatcatag gctcctaaat caagataacc attaccactg
     1561 agattaaaga caataacctt atcttctccg gtttctttgc atttcttagc ttcttcaata
     1621 gccgccttaa ttgcatgagc actttcagga gcgggtaaaa ttgcctcggt cttggtgaaa
     1681 agaacagctg agtcaaaaat ttcattttgc ttataggatt gagcttcgat aatacccgca
     1741 ttatacaata aacttaccaa aggagccata ccatggtatc gtagaccacc ggcatgaatt
     1801 cctggcggga taaagtcatg accgagggta tacattttta aataaggtgt catcccagca
     1861 acatctccta agtcgtattc atatttccca gttagcaaag tggaacaaga tttgggttca
     1921 accgctattg ctcgaaaatt cttcgtacca gcaattttat cacgcaggaa tggaaaagcc
     1981 attcctccaa agttgctacc acccccaaca caaccaataa caatatcggg atacttctct
     2041 ccaacctttt ccaactgcag ttttgtttct tcaccaatta ttgtttggtg aagaagaaca
     2101 tggttaagaa cactccccag actatatttg gaatgaggag tattcactgc atcttcgatg
     2161 gcctcactga ttgctattgc caaactacct attgaatcgg gagtttcatg aagtatatca
     2221 ctcccagttt tagtgttctc actggggctg ggaaatacat tagcacccca agtttgcatt
     2281 aatacccggc gataaggctt ttgacgatag cttacattta ccatataaac cgtgcactcc
     2341 ataccaaaat aatggcaagc catagaaagt gccgaacccc attggccggc accagtttca
     2401 gtagtcaaac gtttagttcc atcctttttg ttataataag cctgtgctac tgcggtattt
     2461 ggtttgtgac tcccaaccgg gctgtcgcct tcatatttaa aatagatacg ggctggtgtt
     2521 tgtaaggctt tttccaaccg tcgagcacga tacaagggtg agggacgcca gaggcggtat
     2581 atatcaagca cttcaccagg tatatcaatg aaacgctggc tgcttacttc ctgctgtatt
     2641 aggctcggtg gaaaaattgg ttccaaatct tgaggagtaa ccggttcttt tgtgcctgga
     2701 tgcaacgggg gatcaagagg ccgtggtaaa tctggtatga tattatacca gatggtcggc
     2761 atctcccttt cgctcaggta tattcggttt gcctcttcca tggtaatcac ctccggtgtt
     2821 ttttttattg gttcatttga aaaccaatac ccttattatc gtttgatatt ttttataaaa
     2881 tctcttctca acatcttgta tttagaacaa tttcacgaat tgcagttttt acatctacca
     2941 attttattca atactgttct tttttcataa agtacgtctt gctatgtcta gctttctatt
     3001 ctgaaaatac catcatttga attctttaat cggtcatcct gaggcttcgc ttttcgaggg
     3061 cgtaaggacc tcatctgaca ccaccggcgt catcctgagt ggtgctttcc cgtgtgagg
//
LOCUS       DAOSOL010000173         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_31497_length_3101_cov_2.999343, whole genome shotgun sequence.
ACCESSION   DAOSOL010000173 DAOSOL010000000
VERSION     DAOSOL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31497_length_3101_cov_2.999343"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK171_04830"
     CDS             <1..196
                     /locus_tag="PK171_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05545.1"
                     /translation="AFANSPKVIATGMYPIKIGSDAKMPLCNSLECSVLVGTMLFAIL
                     GMSIMGTGRKYQYSVIEDFL"
     gene            232..615
                     /locus_tag="PK171_04835"
     CDS             232..615
                     /locus_tag="PK171_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT05546.1"
                     /translation="MTNTVQNFPRGRPGVQRILEPILLLFLTEKPSYGYELLEKIRNV
                     IPGWEEPDVGALYRFLRRLEKEQLLISNWSVEKIGPARRIYHITESGERYLQLWMERL
                     EKNRNLIDSLLDQYKKLTRQQPPEI"
     gene            complement(634..1425)
                     /locus_tag="PK171_04840"
     CDS             complement(634..1425)
                     /locus_tag="PK171_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT05547.1"
                     /translation="MPVQMNPWMISKIVLWVLIAIVSTSFLRKNKVTSKVRNYFLVGG
                     VIIFGFIYGVEPNPVSSLRNLFKNIFLQHRLLPAILLVFVILLLVSWWSNKSICGWGC
                     QFGLLQDLLYRVPSRKRKPPIRLSQIIRFFFFVGLIIGLIMWKIDWLAFVDPFGIFRW
                     SFSRPVILGMSFVSAVLILSVFIYRPWCHFFCPFGFISWIVEQKSLNKPVINLETCKQ
                     CQLCVKACPGNAMEDILHNKKWRSDCFACGACIEACRFHAIQWGK"
     gene            1626..1976
                     /locus_tag="PK171_04845"
     CDS             1626..1976
                     /locus_tag="PK171_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05548.1"
                     /translation="MNERERVMNALEFNTIDKVPYHLDFTVPARNKMIQYLNDQNFEQ
                     NIGNHLAFTKALPLDAFQEVKPGFIRDEWGVVWNRTIDPDIGNPIPVFSQPILKGYRF
                     PDPDDQVQGDHRNR"
     gene            1942..2484
                     /locus_tag="PK171_04850"
     CDS             1942..2484
                     /locus_tag="PK171_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05549.1"
                     /translation="MIKSKVITETAKAKKMPLVDAAIDLNEHIENLKTELQILKDALV
                     KEAVEKIEQSFEDGVFVKTVDINGSTNKIQIQRQDRYSALAEAMIDPLKEIFHSKFDL
                     MFKIETESKIKSEKIEALKALLGDKYETFVEEATTVKPTSEFQYNYFLMKDNLDAEQK
                     DTVQKVLDAAQSTPAVKFPK"
     gene            2548..>3101
                     /locus_tag="PK171_04855"
     CDS             2548..>3101
                     /locus_tag="PK171_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05550.1"
                     /translation="MAIRIGCDPEFAVIDNHDSYIRFTYHKNLDCGFVKDDHCGAVGE
                     LNPNSSTNPAEVVENLRTLIKEIKREYPTLRVIGGGGAKYRISTGGHIHLSGLTNLDF
                     QGYENYSWHRRSTRNHIRPTTKGNKLVLALDAFIARPMQRLQNGKRADKSYNLLSKIK
                     IKSYSNGSDGFEYRSTPSWLTSPKV"
BASE COUNT         1038 a          641 c          586 g          836 t
ORIGIN      
        1 ggcttttgct aatagcccaa aagttatcgc aactggtatg tatccgataa aaataggaag
       61 tgatgccaaa atgcctttat gcaactcatt ggaatgttca gttttagtcg gaacaatgtt
      121 gtttgccatc ttgggcatga gtataatggg aacaggcagg aaatatcaat atagtgtgat
      181 agaggatttt ctttgatttt tgttaattca cttctttttt gagggataac catgacaaat
      241 actgttcaaa attttcccag aggtcgtcca ggagtacagc gaattttgga accaatacta
      301 ctcctttttc ttacagaaaa accctcttat ggctatgagc ttttagagaa aatccgaaat
      361 gttatacctg gatgggaaga gcctgatgtt ggagctttgt atcgctttct aagacgttta
      421 gaaaaagaac aactattaat atctaattgg tcggtggaaa aaattggacc ggctcgacgt
      481 atttatcaca ttaccgaaag cggtgagaga tatctccagt tatggatgga aagattagaa
      541 aaaaaccgca acctcatcga ttctcttctc gatcagtata aaaaacttac tcgtcaacaa
      601 ccacctgaaa tctaaataca accaatgaaa atattatttc ccccattgaa ttgcatgaaa
      661 acggcaggct tcaatacaag ctccgcaagc aaaacaatct gaccgccatt ttttgttgtg
      721 caatatgtcc tccatagcat ttcccggaca ggctttcaca caaagttgac attgtttaca
      781 ggtttcaaga ttaataaccg gtttatttaa ggatttttgt tcaactatcc aactgatgaa
      841 tccaaaagga caaaagaaat gacaccaagg tcgataaata aaaaccgaaa ggattaaaac
      901 cgctgacacg aaactcatac ctaaaataac cggacgagaa aaagaccagc ggaaaattcc
      961 aaaaggatca acaaaagcga gccagtcgat tttccacata attaacccaa taatcaaccc
     1021 cacaaaaaag aaaaacctta taatctgaga aagacggatg ggtggtttcc ttttccgaga
     1081 aggaactcga taaagaaggt cctgcaacaa cccaaactgg catccccaac cacaaatcga
     1141 tttgtttgac caccaagaaa ccagcaaaag aattacaaac accaataata ttgctggtaa
     1201 cagtcgatgt tgtaagaata tatttttaaa taaatttctt aaagaactta ctggattggg
     1261 ctcgactcca taaataaacc caaaaattat cactcctcca acaagaaaat aattacgtac
     1321 cttggaagta actttatttt tcctcaaaaa agaggtgctt actatggcta tcaacaccca
     1381 taaaacaatt tttgaaatca tccacggatt catctgaacc ggcattttac caccgcctaa
     1441 accttaaggg tcattactat atgtatttat catatatata ttattttctg atgtcaactt
     1501 ctattatctt gttttagtga aatgtttcca ttccttatat taattttatt ctctcaaatc
     1561 tttgaaaaat tcacttcttt catttacact gataaaataa ataccatcac cttggaggga
     1621 atggcatgaa tgagcgcgaa agggtaatga atgctcttga gtttaatacc attgataagg
     1681 ttccttacca tttagatttt actgttccag cccgtaataa aatgattcaa tacttgaatg
     1741 atcaaaattt tgaacaaaat atcggcaacc atttggcttt taccaaggca ctgcctctcg
     1801 acgcctttca agaagtaaaa ccagggttta tccgtgacga atggggcgta gtatggaatc
     1861 gtaccattga tccggatatt ggaaacccga ttccagtctt ctcccaacca attctaaaag
     1921 ggtatcgatt ccccgaccca gatgatcaag tccaaggtga tcaccgaaac cgctaaggcg
     1981 aaaaagatgc ctttggttga tgcggcgatc gatctcaacg aacacatcga aaatctgaag
     2041 accgaacttc aaatcctgaa ggatgccttg gtcaaggaag ctgtggaaaa aatcgaacag
     2101 tctttcgaag atggagtctt cgtaaaaacc gttgatatta acggtagcac caacaaaatt
     2161 cagattcaac ggcaggatcg ttattccgca ctggccgaag caatgatcga tcccctgaag
     2221 gaaatctttc attcaaagtt cgacctgatg ttcaaaatcg aaaccgaaag caaaatcaaa
     2281 agtgaaaaga tcgaggctct taaagccctt cttggtgaca aatacgaaac cttcgtggaa
     2341 gaagctacga cggtgaagcc gacctcggaa ttccagtaca actacttcct gatgaaggac
     2401 aacctggatg ccgagcagaa agatacggtt cagaaagttc tggatgccgc ccaatcgaca
     2461 ccggcggtca agttccctaa gtaatttcaa acaaggaagg gcgaaagccc ttcccaaaat
     2521 gaaaaggtaa aaacgtcgag gtgaattatg gctatcagaa ttggatgtga tccggagttt
     2581 gcagtcattg acaatcatga ctcttacata cgtttcactt atcacaaaaa cctggactgt
     2641 ggatttgtaa aagacgatca ttgcggtgcg gttggcgaac tcaatcctaa tagtagtacc
     2701 aatcctgcag aagtggtcga aaatcttcgc actcttatta aggaaatcaa gcgcgaatat
     2761 ccgacactga gagtcattgg cggcggtggt gcaaaatatc gtatttcaac tggtggtcat
     2821 attcatctca gtggtcttac caatctcgat tttcaaggtt atgaaaatta ttcttggcat
     2881 cgccggagta ctcgtaatca cattcgtccg acgaccaaag gtaacaaact ggttctggct
     2941 ctggatgctt tcattgcaag accgatgcaa agacttcaaa atggaaagcg agcagataag
     3001 tcttacaatc tcctctccaa aatcaagatc aaatcgtatt cgaatggttc agacggtttt
     3061 gaatatcgta gtactccttc gtggttaaca agcccgaaag t
//
LOCUS       DAOSOL010000174         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_32849_length_3001_cov_4.386286, whole genome shotgun sequence.
ACCESSION   DAOSOL010000174 DAOSOL010000000
VERSION     DAOSOL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32849_length_3001_cov_4.386286"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PK171_04860"
     CDS             complement(<1..618)
                     /locus_tag="PK171_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT05551.1"
                     /translation="MDRKTEIKFYFMIGFVLYFLAISMIITGAALPKWMDIFQVTAGR
                     AGRLFFLYYLTYVITTFSSGFLCDHFGKKPIIVLSQLFLGIGFFCISTAHSFLFIELG
                     MLIMGLGGGFCEAPMTGFISQVFNGREGFALNMSQIFFGIGAASGPFLTGYLLGIGFS
                     WRYLYLIPGIVSIGLFFLIYFEKKLFQKEKKGITSGSILILYQRWK"
     gene            complement(998..>3001)
                     /gene="pdhA"
                     /locus_tag="PK171_04865"
     CDS             complement(998..>3001)
                     /gene="pdhA"
                     /locus_tag="PK171_04865"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOT05552.1"
                     /translation="SKDKLLQMYKDMLRIRRFEETVSDLFSQDKIRGTTHLYIGEEAV
                     AVGACNAIHPDDYITSTHRGHGHCIAKGATLREMMAELFGKITGYCKGKGGSLHIADL
                     NAGNLGANGIVGGGIPIATGSGLTGKYKKTGKVTVCFFGDGASNTGAFHEAVNMAATW
                     KLPVVYVCENNLYAMSTPVREAFPILDIAERGQAYGMPGIVVDGMDVLAVMEAVEQAA
                     EKARRGEGPTLIECKTYRYLGHSKNDPRAYRTKDEEKQWKERDAIKRFRKWLLENTVA
                     TEEEIQTIDEEVENEIAEAVEFAESSPYPPLEEIVKDVYVEEDFAEKERKKGAKIVLS
                     FNEYSDNQKLRNITYRDALNEALREELNHDPNVVLIGEDIGLYGGAYGVTRGLWQDFG
                     DERVRNTPISEAAIVGCCVGAAITGLRPVGELMYVDFAGLAMDQIYNQGAKIRYMFGG
                     KARVPMVIRTEGGCGRSSGAHHAQSLEAWFMHVPGLKVVMPATPFDAKGLLKSAVRED
                     NPIIFIEHKMLYNTKGLVPDDEYLIPIGVADVKKSGTDVTVIAYSRMLLVAMEAAEIL
                     EKEGIHIEVIDPRTLLPLDIDTIVTSVKKTGKVVIVSEACKTGGPGGEIGMQIMENAF
                     DYLDAPMVRLCAADAPVPCSRYLEDNSIPQAKDVVKTVKELLE"
BASE COUNT          912 a          683 c          490 g          916 t
ORIGIN      
        1 tttccaacgt tgatataaaa tcagaatact cccactggta attccttttt tttctttttg
       61 aaataatttt ttttcaaagt aaatgagaaa aaataaacca atagaaacaa tcccggggat
      121 gagatataaa tatctccagc taaaacctat tcctaacaga tacccagtca aaaaaggacc
      181 gctggcagct ccgattccaa aaaaaatctg actcatgttc aaggcaaatc cctctcgacc
      241 attaaaaact tgcgagataa aaccagtcat gggtgcttca caaaaacctc cacccaaccc
      301 cataattaac atccccaatt caataaaaag aaaagaatga gctgtgctta tacaaaaaaa
      361 accgatccca agaaataatt gactcagaac tattattggt ttttttccga aatggtcaca
      421 aagaaaacca ctggaaaaag tggtgatcac ataggttaaa taataaagga aaaaaagtct
      481 tccggctcga ccggcggtta cttggaaaat gtccatccat ttagggagag ccgcaccagt
      541 aatgatcatt gagattgcta aaaaataaag tacgaaacca atcataaaat aaaacttgat
      601 ttcagttttt cgatccattt catgttcctt agtttttttt aaaaagtgac gagtgttatc
      661 gaactaacac ataaatatta aaaaacatct cctttaattt aaagtttttt aataagaatt
      721 aaatgtttta tgaattttcc atactttttt attcctactt ggaaaatcag ggaaattgaa
      781 ttctaattta tgaaagtcac tcttatcctc aactggttct attgctctat cgcatgaaca
      841 attattctgc tcactcattt agaggtttta caaatttaat tctgctaaaa tttatataaa
      901 aatttaaggg gcgcaataga attgcgcccg ttggggattt ctccataaaa tataacttct
      961 attaagctta aatatcactc ttgccaaata atttcaatta ttctaataac tcttttactg
     1021 tcttaactac atctttagct tggggaatgg agttatcttc taaatatcgg ctacagggaa
     1081 ccggtgcatc ggcggcacat aatcgcacca tgggagcatc aagataatca aaggcattct
     1141 ccataatttg catcccaatt tctcccccgg gtccaccggt cttacaggct tcggaaacaa
     1201 ttactacttt tccggttttc tttactgaag ttacaatggt atcgatatct aaaggtaaca
     1261 gggttcgggg gtcaataact tcaatatgaa taccttcttt ttccaatatt tcggcggctt
     1321 ccatggcaac caataacatc cgcgaatatg caataaccgt tacatcagta cctgattttt
     1381 taacatcagc aactcctatt gggataagat actcatcatc aggaactaaa cctttggtgt
     1441 tatacaacat tttatgttcg ataaaaatta ttggattatc ttctcgaaca gccgacttga
     1501 gaagaccttt tgcatcgaaa ggagtggctg gcattactac ctttagacca ggaacatgca
     1561 taaaccaggc ttccaggctt tgagcatggt gggctcctga agatcggccg cagccacctt
     1621 cagtacgaat aaccattgga actcgagctt tcccaccaaa catataacga atctttgctc
     1681 cctgattata aatttggtcc atagctaatc cagcaaaatc cacatacatc agttcaccaa
     1741 ctggacgaag accagttatc gccgcaccga cacaacatcc aacaatggcc gcctcagaga
     1801 tgggggtatt tcttactctt tcatcaccaa aatcctgcca caatccccga gttactccat
     1861 aagccccacc gtataaacca atgtcctctc caatcaatac tacgttgggg tcatgattga
     1921 gctcttctct taaggcttca tttaatgcat cacgataggt aatatttcta agtttttgat
     1981 tatcgctata ttcgttaaaa gagagaacga ttttggctcc tttttttcgt tctttttcag
     2041 caaaatcttc ttcaacatag acgtctttga ctatttcttc cagcggggga taggggcttg
     2101 attcggcaaa ttctaccgct tcagctattt cattttctac ttcttcatca atagtttgaa
     2161 tttcttcttc ggtcgcaaca gtgttttcaa gcaaccattt tcgaaaccgc tttattgcat
     2221 cacgttcttt ccactgtttt tcttcatctt tggtccggta agctcttgga tcgtttttgg
     2281 agtggcctaa gtatcgatag gtcttacatt caataagtgt tggaccttct cctcgacgag
     2341 ctttctctgc tgcttgttca acagcttcca taacggctaa tacatccatc ccatctacta
     2401 ctatacctgg cattccataa gcttgacctc gctcagcaat atctaaaata ggaaaagctt
     2461 ctcgtaccgg cgttgacatg gcataaagat tattttcgca tacataaacg accggtaatt
     2521 tccaggtagc cgccatattc actgcttcat ggaaagctcc ggtattggat gctccatcgc
     2581 caaaaaagca tactgtaacc ttcccagttt ttttatactt accagtcaat cctgaaccag
     2641 ttgcgattgg gataccaccc cctacaatcc cattggcacc aagatttcca gcgttaaggt
     2701 cagcaatgtg aagtgatcct cctttaccct tgcaataccc ggttattttc ccaaataact
     2761 cggccatcat ttcccgcaaa gtagcacctt tcgcgataca atgaccatgc ccacgatggg
     2821 tagaggtaat atagtcatcc gggtgaatcg cattgcaggc tccaacagca acggcttcct
     2881 cacctatata aagatgagtt gtccctcgga ttttatcctg agaaaaaaga tcacttaccg
     2941 tttcttcaaa acggcggata cgaagcatat ccttatacat ttgaagtaat ttgtcttttg
     3001 a
//
LOCUS       DAOSOL010000175         2980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_33126_length_2980_cov_3.935385, whole genome shotgun sequence.
ACCESSION   DAOSOL010000175 DAOSOL010000000
VERSION     DAOSOL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33126_length_2980_cov_3.935385"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1223
                     /locus_tag="PK171_04870"
     CDS             165..1223
                     /locus_tag="PK171_04870"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05553.1"
                     /translation="MNGKDRVKTALKHQEPDRVPISCQYTPEIQRLLEDYTGQKGIDV
                     NVAMGDDAVIVWEGMVNLFNGKIKEGEIYDTEWGIRFLKKGLYNEICHNPLSIATTVD
                     ELKKYPFPLPVSQELIDRAANIIKKYSDNYAIIGAVPLTMWEGAIHLRGYQQMLEDML
                     TNLRMAETLLDRVMEYHFEIAMALIDLGIDILWLGDDVGMQSGMLISPNLWRQVFKPR
                     WKMMFQAFKKRNPNLIIAYHSDGGIRPIIPDFIEIGLDLLNPLQPLSIGMDSFELKKD
                     FGKDLSFFGGIDIQEALPFGTPRMVRDEVKKRIDAFAPGGGYLCGPTHNIQADTSLEN
                     ILTMYHTIQNYGQYTVRY"
     gene            complement(1247..2365)
                     /locus_tag="PK171_04875"
     CDS             complement(1247..2365)
                     /locus_tag="PK171_04875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT05554.1"
                     /translation="MSLFAFSIAFMLHLLLFATAPLSTIIMEEMNLSHAGFAFIFGIA
                     MISLVASRIPWGLIGDHIGFRNAFRIALPISAIAAVIRPFTQSYATLLMTQLALGFGL
                     SSILPILPLLVKKSFHKHSTGFATGIYIAGFAAGNATAIAFTPSLLPILNWRNILLVY
                     AMLSVLIAIFWWLFIPETHLKTETIKITQFKAILKDRSLWVLLMLVIACMGGYDTLAT
                     WVPRVIEMKNFRPSLATFLPLGFFFSGPITGFISDRLKNRNFLLSFMGIATLLATLGI
                     NSNNYSIFNLSLFLAGFCLNSILTISLAIPAEHERYATSVGGVVGVISSLGNIGPLAL
                     PIIFGSLIDLTGAYRASIVFIALFSGLIITLCSRSKYF"
     gene            complement(2466..>2980)
                     /locus_tag="PK171_04880"
     CDS             complement(2466..>2980)
                     /locus_tag="PK171_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOT05555.1"
                     /translation="QGLIAIDLQDSRLEWAKRLGAQFILNPKRDDCEQFIQDLTHNQG
                     VDIAIEITGKLKGLEMASLIIRNASFYGYQGRGRIIASSFYAGDQIMSNTLGYQLMFK
                     SPIVASVHPWHSLNFIEDVAKGVWGYQKHFLPLDQLVTHEFCLEDLAKGFEAASGKDI
                     DYLKGVIIME"
BASE COUNT         1040 a          530 c          596 g          814 t
ORIGIN      
        1 ttatcgatca atcgattttg taactggaaa taaccaaaac aatagtaaaa accgagctgt
       61 taataaaaaa aattaaaagg tgaatcatgc tatgaaaata acacctaact atcacctagt
      121 ctttatttga tattcggtac gagatacgaa aggagacaag aaagatgaat ggaaaagatc
      181 gagtcaagac tgccttaaaa caccaagaac cggaccgcgt tcccatctcc tgccaatata
      241 cacccgaaat ccaacggctt cttgaagatt atactggaca aaaaggtata gacgtcaatg
      301 ttgccatggg agatgatgcg gtcattgtct gggaaggaat ggttaatctt tttaatggaa
      361 agattaaaga aggggagatc tatgacactg aatggggaat tcgattttta aaaaaaggtc
      421 tttataatga gatatgtcat aatccgctga gcatagccac cacagttgat gagctaaaaa
      481 agtatccttt tcccctacca gtaagccagg agttgatcga tagagcagcg aatattatta
      541 aaaaatacag cgataattat gccatcattg gagcagtgcc tttaactatg tgggagggag
      601 caattcatct gcgaggttac caacaaatgt tggaagatat gcttacaaat ttaagaatgg
      661 cagagacatt attggatcga gtcatggaat atcatttcga aattgctatg gcccttattg
      721 atttgggaat agatattctc tggctgggtg atgatgttgg aatgcaaagt ggtatgctta
      781 tttctcccaa tctctggagg caagtattta agccacgctg gaaaatgatg tttcaagcat
      841 tcaaaaaaag aaatcctaat ctcatcatcg cttatcattc tgatggcgga atccggccga
      901 tcatccctga ttttatcgaa atcggattgg acctgctcaa tcctctccag cctttaagca
      961 tcggaatgga ctcgtttgaa ttaaaaaaag attttggtaa ggatttaagt ttttttgggg
     1021 gcattgatat tcaagaagcc ttaccattcg ggactccgcg aatggtaaga gatgaggtga
     1081 aaaagagaat cgatgccttt gcccctggtg gaggttacct ctgtgggcca actcataata
     1141 ttcaagctga tacttctctc gaaaatattt taaccatgta ccatactatt caaaattatg
     1201 gccaatacac tgtaagatat tgattcgtgc aagaagatac cgggaactaa aaatattttg
     1261 atcgtgaaca aagagtaata attaaacctg aaaataatgc aataaagaca attgaagctc
     1321 gataggctcc agttagatcg attagagaac caaagataat gggtagagca agtggaccaa
     1381 tatttcccag cgatgagata actccaacta ccccgcctac tgaagtagca tatctttcgt
     1441 gttccgccgg aatcgctaag ctgatggtta aaatactgtt taagcaaaac ccggctaaaa
     1501 ataagcttaa attaaatata ctataattat tagaattgat tccaagagtt gccaaaagag
     1561 tggctatacc cataaaggat aataaaaagt tacggttttt caaacgatca gaaatgaatc
     1621 cagtaattgg acctgagaag aaaaaaccta aaggaagaaa agtggccaaa gaaggtcgaa
     1681 aatttttcat ctcgattact ctgggaaccc aggttgccaa ggtgtcgtag cctcccatac
     1741 atgctataac caacattaga agaacccaaa gagagcggtc tttgagaatc gctttaaatt
     1801 gagtaatttt aatagtttcg gttttaagat gagtttcagg aatgaagagc caccaaaaaa
     1861 ttgctattaa aaccgacagc attgcataga ctaaaagaat atttctccaa tttaaaattg
     1921 gtaataaact tggtgtaaac gcaatagcgg ttgcattccc agctgcaaaa ccggcaatat
     1981 aaattccagt ggcaaaacct gtggaatgtt tatgaaagga ttttttaact aagagcggaa
     2041 gaatagggag aattgatgat aaaccaaaac caagagccag ctgagtcatc aaaagtgtag
     2101 cgtagctttg ggtaaaaggc cgaatgactg ctgctattgc tgaaatggga agagcaatcc
     2161 gaaaggcatt cctgaagcca atatgatcac caattaatcc ccaggggata cgagaagcaa
     2221 ccaagctgat catagcaatc ccaaagataa aagcaaaacc ggcatgagaa aggttcattt
     2281 cttccataat aattgttgaa agcggtgcgg ttgcaaataa aagaagatga agcataaaag
     2341 caattgaaaa tgcaaaaagc gacattttta attgatgaaa attaattgaa ttttttatca
     2401 taaaaagaat cctttctttt agtggttgtt atgacattat aaatacggtt aaaaatttat
     2461 accggttatt ccatgataat aacgcctttt agataatcga tatcctttcc agatgctgct
     2521 tcaaatcctt tagctaagtc ttcaaggcaa aactcatgag tgaccaactg atcaagggga
     2581 aggaagtgct tttgatatcc ccagactcct ttggcaacat cttctataaa attgagagaa
     2641 tgccagggat gaaccgaggc gactatgggt gacttaaaca tcagctgata accaagagta
     2701 ttgctcatta tttgatcacc agcataaaat gacgatgcaa tgattctacc tctaccctga
     2761 tacccataaa aggatgcatt acgaattatc aaagaagcca tttccaaacc ttttaatttt
     2821 ccagttattt ctatggcaat atctactcct tgattatgag ttaaatcttg gataaattgt
     2881 tcacaatcat cccgttttgg attgaggata aattgagctc ccaatctttt tgcccattcc
     2941 agacgtgaat cttggagatc gatagcgatt agcccttgaa
//
LOCUS       DAOSOL010000176         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_33175_length_2977_cov_3.928816, whole genome shotgun sequence.
ACCESSION   DAOSOL010000176 DAOSOL010000000
VERSION     DAOSOL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33175_length_2977_cov_3.928816"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PK171_04885"
     CDS             complement(<1..331)
                     /locus_tag="PK171_04885"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="catalyzes the reversible phosphorylytic cleavage of
                     uridine and deoxyuridine to uracil and ribose- or
                     deoxyribose-1-phosphate; involved in the pyrimidine
                     salvage pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOT05556.1"
                     /translation="MKYYAYHLSMAKDDLRGATIALIPGAPERVVTIAQEFGPYQEIA
                     FHREYRSVIADDGKDRILVVSSGIGGPSLAIAIEELAQLGIQTFIRVGTSGAIQDFIQ
                     EGDLIISE"
     gene            1019..2704
                     /locus_tag="PK171_04890"
     CDS             1019..2704
                     /locus_tag="PK171_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05557.1"
                     /translation="MNQLEKIISLAVIEMKASPCSITINNQQNLNITPGMTILEIAHK
                     SFPDTYQNFMAARLNGEIVDLNTKVTKPSDICFLDFSQPEGHRTYLRSLLFMLSYSIN
                     TLYPKAHLHVLHSMSDGLFCEITGIVNLTTEHLDNINQKMQELARLNLSFEREIVDWD
                     KAKNIFHRQNREDLNRLFRYWRTTAITLYRLNKYYDYYYGPLCPSTGYIKNYGFVLVP
                     PGFFLQYPRATDTERLPTYIFRPKLFTTFQEAGQWAKIMEIKNVGDLNESIVHGQGRE
                     IVSMTEAFHEKKIAQIADLITQKRGSLKTVFIAGPSSSGKTTFARRLYIQLRVNGWKP
                     ETISLDDYFISKKDIKTWDNQYYERPEALDLELFEQQIEELIDGKEVEIPRYNFITGS
                     REPQGAKMQLGHDGIIIIEGLHALNPLLSKNIPEERKFKIYASAITQLNIDNHNRMST
                     TDCRLIRRLVRDSQFRGSTTEEVFKTWLHVHAGEEKYIFPYQEDADIFFNSSLIYELS
                     ILRQYAEPLLRAITPENETYLEAYRLYAFLNNFIPLQSSFVPSNSILREFIGT"
BASE COUNT          901 a          686 c          535 g          855 t
ORIGIN      
        1 cctcactgat aattaaatcc ccttcctgga taaaatcctg aattgctcca gaagtaccta
       61 ctcgaataaa agtttgaatt cccaactgtg ccaattcttc aatagcaatt gccagtgaag
      121 gacctccaat acctgaagat acgactaata ttctatcctt cccatcatca gcgataacac
      181 ttcggtattc ccgatggaat gctatttctt ggtaaggccc gaattcttgg gcaatggtta
      241 cgaccctctc tggcgctcca gggataagtg ctatagtagc tccccgaagg tcgtcttttg
      301 ccattgagag atgataagca taatacttca tcgctacctc cccttaccaa aaaattttca
      361 accgattcca tcatatccta acacaaaaag aatgaggaca gtagatatta aaaaacaacc
      421 taataaaaga aaacccatgg gtatgataaa tcaagccccc taccaaagga tatttttatg
      481 taggggcaca attcattgta cccgtttaat gtatcacctt accatggcat gtcgaatctt
      541 aggttttcat ttctatccgg tatcaccaaa tggcatgagg gtttatcctg ggaacataat
      601 attgagtaat aagtgatcaa ataaaaatga gaaagaatga attcaacaat tcccctcctt
      661 ggaggggtgc cacttttgtg gcggggaggg tgtctttcga ttttccctcg ttatcctgag
      721 aaaaggctaa agaaatacga ataaaagatg agatcctcac ggcttctgaa tacaaagcct
      781 caggatgtga cacctgtggt gtcagatgtg accctataat gtctggtaaa agacaccgga
      841 ctcattaaag taacaaaatg gctaaatgag attgccacgt cgttcaacca aagaacggtt
      901 ggattcctcg caatgacagt tttagatagt tattttactt tctggcaata acacaaatcc
      961 agcatttgtg atgatcttat taatcagatt ttacagagta gtcaagactg agtacaatat
     1021 gaatcaatta gaaaaaatta tatctttggc ggtgattgag atgaaagcat ccccgtgttc
     1081 gattaccatc aataatcaac aaaatttaaa tatcactccg ggtatgacta tcctcgaaat
     1141 cgcccacaag tcttttcccg atacttacca aaattttatg gcagcccgct taaatggaga
     1201 aatagttgac ctcaacacca aagttactaa accaagtgat atttgcttct tggatttctc
     1261 ccaacctgaa ggacatcgta cctatcttcg cagtttactg tttatgctca gctacagcat
     1321 taacaccctt tatcctaaag cccatcttca tgttcttcat tccatgagtg atggtttatt
     1381 ttgtgaaata acaggaattg taaatttgac taccgaacac ttagataata tcaatcaaaa
     1441 aatgcaagaa ttagcccgat tgaatctctc ttttgaaaga gaaatagttg actgggataa
     1501 ggcaaaaaat atttttcatc gacaaaatcg agaagacctc aatcggcttt tccgctattg
     1561 gcgcacaaca gcaataactc tttaccgcct taacaaatat tatgattatt actatgggcc
     1621 tctttgccca tcaaccggct atataaaaaa ttatggattt gttttggttc cacctggctt
     1681 ttttcttcaa taccctcggg cgaccgatac cgaacgtctt cccacctaca tttttcgtcc
     1741 caaattattt accactttcc aggaagccgg tcagtgggca aaaatcatgg agattaaaaa
     1801 tgtcggagat ttaaatgaat cgatcgttca cggccaaggt cgagaaattg tctcgatgac
     1861 cgaggctttc catgagaaaa aaatcgccca aatcgccgac ctcatcactc aaaaaagagg
     1921 aagtctgaag acagtcttta tcgccggtcc ttcctcttca gggaaaacca cttttgcccg
     1981 gcgtttgtat attcagttaa gagtgaatgg ttggaaaccg gagaccattt ctcttgacga
     2041 ttactttatc tccaaaaaag atattaaaac ctgggataat caatactacg agcgcccgga
     2101 agctcttgac ctcgagcttt ttgaacagca aattgaagaa ctcatcgatg gaaaggaagt
     2161 tgaaattcct cgctacaact ttatcaccgg aagccgggaa ccccagggag ctaagatgca
     2221 attgggtcac gatggcatta tcatcatcga aggacttcac gccctcaatc ctttgctcag
     2281 taaaaacatc cctgaagaaa gaaaattcaa gatttacgcc agcgccatta cccagctcaa
     2341 tattgataat cacaaccgaa tgtcgaccac tgattgccgg cttattcgcc gtttggttag
     2401 agacagtcaa tttcggggaa gcaccactga ggaagtcttt aaaacctggc tacatgttca
     2461 tgccggagaa gaaaaataca tctttcctta tcaagaagat gccgatattt ttttcaattc
     2521 atcacttatt tatgaattaa gcattcttcg ccaatatgcc gaaccgcttc tccgggcaat
     2581 cactcctgaa aatgaaacct acttagaggc ttatcgactc tatgcctttt taaataactt
     2641 cattcctctt caatcttctt ttgtaccatc caattcaatc ttgagagaat tcatcggaac
     2701 ttaaatccgt cactacaagg agcaaagcta tctggcaatc acactgccaa aatccgtgaa
     2761 gagtgagccg tgagccgtaa aattttcacc ctcatcctga ccttctccca tttctctttc
     2821 tttcctctcc cttggtggga gaggattgag gtgagggggc ctcaactttc ctcaaccttt
     2881 gtctcaaagc ttcaaacttg gatcttcatc ctgggttttc atcctcatcc tgaccttctc
     2941 ccatttctct ttctttcctc tcccttggtg ggagagg
//
LOCUS       DAOSOL010000177         2970 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_33299_length_2970_cov_3.836021, whole genome shotgun sequence.
ACCESSION   DAOSOL010000177 DAOSOL010000000
VERSION     DAOSOL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33299_length_2970_cov_3.836021"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..>2970
                     /locus_tag="PK171_04895"
     CDS             43..>2970
                     /locus_tag="PK171_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT05558.1"
                     /translation="MKMKLLVIWIFVIFFVLSSQSTFSMAQTPSSPINEADLTEEKAS
                     SVPSYDESRQADVNDSLSCFDFQFTCEYGTPEEVQEAIKNGADINCQDEELGWTPLMS
                     AMLNESGQEIVTLLVGAGANVNVTSWSGINALMLAAESSESSLVKYLLDNGAKEYIND
                     TESEYGFTPLIWAAKNNDTNGGEVIQVLIDHGADVSIKDKNGFTAILYAAMLNRNEDV
                     IHTLIQNGVDINQIGPKNVTPLMGAISMNSNINITKLLLDSGADVSVISEEGKKAIDY
                     ALELERFKGTEILRQLAEMSGVEIAQPMVSPDIETAPSPNVEITTTPLPTGIITPEIT
                     PTPVPTQALPSMVYADPSGKFTLNFPTGFVFSTYVADRGMIGANYRTPNEKAYLEVRS
                     FPSSQDLQLYLSQHIKDMALRGESSITSNGKTGNIRIYSRKTEQDELLTIVTTYQEID
                     VALVVINLPIAAYQPSSSWLLGLFKGINWEGKTPIIDGSYSDPDQKFSFKLPDGVHFL
                     YAFEKDFYFNQPYRFLPEADGMVGSTPDSGQVMIASFSTPEKYQDYLKEYDRRVETME
                     YQVHGTSQFGVQGSPAKLTLYSYLAQNQKRAELLAVYEGTLVVISVWLPADNYQSAQT
                     WLLSLFKNLTWKKEETLPSFSPLAQPTPTSQITVTTQPTESLQPTTSPQISSDDLSIE
                     AAVHIEKGDFQKAIDLLKQSIALQPDFGFAYGQLAYCYIQLKLYQEVIDQLSDKLLPK
                     KEYSTAYNLVGFAYENLARLDEALVAYQKAVELDPNYVDAYSNMGFVHFDQEQYQKAV
                     DAFKKASELKPSDPVIFNNLGFSYEKLGQYNEALQAYQEAIRIKPDYIKAHANIGYLY
                     FDQEQYQKAVDALKKSLELEPSDPAILNTLGLSYENLKQYDDAIHAYQEAVRIKPDYV
                     NAYNNLGNLFFDLGQFQNALDNYKKSLILDSTNPYNYYNLGLCYENLKQYDDAILAYQ
                     KAIS"
BASE COUNT          921 a          597 c          572 g          880 t
ORIGIN      
        1 tggttttttt cccaaactaa ccaacaaaaa agagggatat ctatgaaaat gaaactcttg
       61 gttatctgga ttttcgttat tttttttgtt cttagttctc aatcgacctt tagtatggct
      121 caaactcctt cttctccaat aaacgaagca gatctaaccg aagagaaggc atcttcggtt
      181 cccagttatg atgaaagtcg tcaagccgat gtaaacgata gtttatcgtg ttttgatttt
      241 cagtttacct gtgagtatgg aacacccgaa gaagttcaag aagcgattaa gaatggtgcc
      301 gatatcaact gccaagatga agagctgggg tggactcctt taatgagtgc gatgctcaat
      361 gaatcagggc aagagattgt cactcttttg gtcggagctg gtgcaaatgt aaatgtgaca
      421 agctggtcgg gcattaatgc tcttatgctg gctgctgaat ccagtgagag cagcttggtg
      481 aaatatttac tggataacgg agccaaagag tatattaatg ataccgaatc ggaatatggt
      541 ttcactccgc tcatttgggc ggccaagaat aatgatacca atggcgggga ggttatccaa
      601 gttttaatcg atcatggagc agatgttagc ataaaagata agaatggatt tacagctatt
      661 ctatatgcag caatgctaaa cagaaatgag gatgtgattc acaccctaat tcagaatgga
      721 gttgatatta atcaaattgg acccaagaat gtgacgccct tgatgggggc tataagtatg
      781 aattccaata ttaatattac taaattatta ctcgattcgg gggccgatgt ctccgtcatc
      841 agcgaggagg ggaaaaaggc aattgattat gctctggagt tagaaagatt taaaggaact
      901 gagatattac gacaattggc tgaaatgagt ggagtagaga ttgcacagcc aatggtttcg
      961 cccgatatag aaactgcacc ttcacctaat gttgaaataa ctaccactcc attgcctaca
     1021 ggaataataa cgccggagat cacccctact cctgttccaa cccaagctct tccttctatg
     1081 gtttatgccg atccgtcagg aaaattcact cttaatttcc caactgggtt tgttttttcg
     1141 acttatgtag cagatagggg tatgattggt gcaaactacc gcacccccaa tgaaaaagcc
     1201 tatttagaag tacgtagttt cccatcttcc caagacttgc agctttacct ctctcagcat
     1261 ataaaagata tggctttacg gggagaaagt tcgattacct ctaacggtaa aaccgggaat
     1321 atcagaattt actcgagaaa aactgaacag gatgagctcc tcaccattgt tacaacctat
     1381 caggaaattg atgttgcatt agtggttatt aatcttccaa ttgcagctta tcaaccttcc
     1441 agctcctggt tattagggct ttttaaagga ataaattggg aagggaaaac accaataatt
     1501 gatggttctt attctgatcc ggatcaaaaa ttttctttca aacttcccga tggagttcat
     1561 ttcctctatg cttttgaaaa agatttttac ttcaaccaac cttatcgatt ccttcctgaa
     1621 gctgatggga tggtaggcag tacacctgat agcggtcagg tcatgattgc cagtttttca
     1681 actccagaaa aatatcaaga ctatctcaaa gaatatgatc ggcgggttga gacgatggaa
     1741 taccaagttc atggaaccag ccaatttggt gtccaaggga gcccggctaa gcttactctt
     1801 tattcttatt tagcccagaa tcaaaaacgt gctgagcttt tggcggttta tgaaggcacc
     1861 ttagtagtta tatcggtatg gttgcctgct gacaattatc aatctgctca aacttggctt
     1921 ttatctctct ttaaaaatct aacctggaaa aaagaagaga ctttaccttc gttttctcca
     1981 ttagctcaac caacacctac ctctcaaatt acggtaacaa ctcaaccaac tgaatcgtta
     2041 caacccacca cttctcctca aattagctcg gatgacttat caattgaagc tgctgttcat
     2101 attgaaaaag gtgacttcca gaaagccata gatcttttaa aacagtcaat tgctcttcaa
     2161 cccgattttg gttttgccta tggtcaactg gcgtattgtt atattcaatt aaagctttat
     2221 caagaagtaa tcgatcaatt atccgacaaa ctccttccaa aaaaagaata ttcgacggct
     2281 tataatctcg tgggctttgc ctacgaaaac ctcgctcgtt tagatgaagc gctggttgct
     2341 taccaaaaag ctgttgaatt ggatccaaat tatgttgatg cctatagcaa catggggttt
     2401 gtacactttg accaggaaca gtatcaaaaa gccgtggatg cctttaaaaa agcttctgaa
     2461 ttaaaaccca gtgacccagt catattcaat aatcttggtt tttcctatga aaaactggga
     2521 caatacaatg aagcactaca agcttatcag gaagccattc gcatcaagcc agattacatc
     2581 aaagctcatg ccaacattgg atatctatac tttgaccagg aacaatacca aaaagctgtg
     2641 gatgctttaa aaaaatccct tgaattagaa cctagcgatc cagctattct caataccctg
     2701 ggtttatcct atgaaaatct aaaacaatat gatgatgcta ttcatgctta tcaggaagcc
     2761 gtccgcatca agccagatta cgtgaatgct tacaataatc ttggaaatct tttttttgat
     2821 ctgggtcaat ttcaaaatgc tcttgataac tataaaaagt ctctaatttt agactcaacc
     2881 aatccttaca attattataa tctcggtctc tgttatgaaa atctaaaaca atatgatgat
     2941 gctattttag cttatcagaa agccatatct
//
LOCUS       DAOSOL010000178         2918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_34052_length_2918_cov_3.431715, whole genome shotgun sequence.
ACCESSION   DAOSOL010000178 DAOSOL010000000
VERSION     DAOSOL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34052_length_2918_cov_3.431715"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PK171_04900"
     CDS             <1..265
                     /locus_tag="PK171_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HOT05559.1"
                     /translation="WLATNPKLFILDSPTVGIDVGSKSEIYEIIHELARNRMAIIMIS
                     DEIPEIYHNCNRIIVMRDGKITANVNTSDTTEDELRSLVEGRA"
     gene            267..1286
                     /locus_tag="PK171_04905"
     CDS             267..1286
                     /locus_tag="PK171_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05560.1"
                     /translation="MNGRRYTIGDLMKRQESVLFLILFAYCAFVSIVNPTFLALDNIF
                     DIVKSSAGMMVLAMGVLVVLVSGGIDVSFTAMAIFSGYITIRIMIAFGIDSLFLAFLA
                     SAFIGLFLGFLNGIIVHKFRLRPLIVTLGTQNIFIGALSVLFGTKFIPVGQMPKSIVR
                     FGTNSLLSIPLSSGGTANLTLFLVPVALVVLVTWFILNRTMLGKSVYALGSSPVAAQR
                     AGLNIASIRYFVYSYMGVLAGIMGIIYAAEVRSLNPISLVGEELTIIAATVLGGAKLT
                     GGRGTVLGTFFGVLIISVLNTTLILIGLSSSWNNFFVGIIILLSVGITAFRNKKESEK
                     NLIFL"
     gene            1298..2290
                     /locus_tag="PK171_04910"
     CDS             1298..2290
                     /locus_tag="PK171_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05561.1"
                     /translation="MVKISKEMIILFVIFAIVLVGIAILLGGSFYNSRNFVSMGFQIP
                     EFGFLALAMSLAMLTGGIDLSIIGNMNLSGILAALILTNQSLLSTAGTTVTVVLAILV
                     ALLFGLICGALNGILIGYIRIPAMLATIGTMLFYTGVGMAITGGRGVVGFPEIFMLFG
                     NSRFLGLPIPLLIMFIIFALVALALEHTVWGRSVYLVGNNYIAALFSGVRTTKTIILT
                     YMIGGLMCGFSALILISRVNSARIGYGDTYLLQAILVSVMGGINPEGGSGKVLGVLLS
                     ILILQSLSSSFTLFAITPYARGLIYGLMLLIIMIVNYVYGEGSKRSLRKAKIKQ"
     gene            2366..2836
                     /locus_tag="PK171_04915"
     CDS             2366..2836
                     /locus_tag="PK171_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT05562.1"
                     /translation="MQDGELIQIGYLVKDLEGAVNFYMETMNVGPWEIYTFAPPVLRE
                     SMVRGKPSNHEFRIALASPGQIQVELIQPLTGRSIYDEYLEQKGEGLHHVKYYYKDCQ
                     KVIQEFEKKGIPVIQSGKFDEDEYYYLDTLKHYGMMIEIGNGGKIRPPERIISK"
BASE COUNT          824 a          470 c          641 g          983 t
ORIGIN      
        1 atggttagcg actaatccta aattatttat ccttgatagt ccaacggttg gaatcgacgt
       61 tggttcaaaa tcggaaattt atgaaataat ccatgaatta gcccgaaacc gtatggcgat
      121 tataatgatt tctgatgaaa ttcctgaaat ataccataat tgtaatcgca tcattgtcat
      181 gagggatggg aaaataactg caaatgtaaa taccagtgat accaccgagg atgaattgcg
      241 tagccttgtg gaaggaaggg cctaagatga acggaagaag gtatacaatc ggagatttaa
      301 tgaaacgaca agaatcagtg ttgtttctca ttctgttcgc ttattgcgca tttgttagta
      361 tagttaatcc aacattttta gctctggata atatttttga tattgttaaa agtagtgccg
      421 gaatgatggt attggccatg ggagtattgg tggtgttagt ttcaggtggg attgatgtat
      481 ctttcacggc catggcaata tttagtggtt acattaccat ccgaattatg attgcgtttg
      541 gtatcgatag tttatttttg gcttttttag catcggcttt tattggttta tttttgggtt
      601 ttttaaacgg tatcatcgtt cataaattta ggttgcgacc tctcatcgtt accttaggga
      661 cacaaaatat ttttattggt gctttatcgg tattatttgg aactaagttc attccggttg
      721 gacagatgcc gaaatctatc gttcgattcg gtaccaactc tttattatca atacctttat
      781 cgagtggagg aacggcgaat ctcaccttat tcctcgttcc ggtcgccctg gtggtattgg
      841 taacctggtt tattttaaac cgaactatgt tgggaaaaag cgtctatgct ctggggagca
      901 gtccggttgc cgcccaacga gctggattga atatcgcatc catccgctat tttgtttatt
      961 cttatatggg agttttagca ggaataatgg ggattatcta tgcagctgaa gttcgatcgc
     1021 tgaatccgat ttccttagtt ggcgaagaac ttacgattat tgctgcaaca gttctgggcg
     1081 gtgcgaagtt aactggagga agaggtactg ttttaggaac attttttgga gtgctcatca
     1141 tttcagtttt aaataccacc ttaatcctta ttggtctttc gtcttcctgg aataatttct
     1201 ttgttggaat catcattctc ctcagtgttg gaataacagc ttttcggaat aaaaaagaga
     1261 gcgagaagaa cctgatcttt ctgtgaggaa aacaccaatg gttaaaatat ccaaagaaat
     1321 gatcatcctt tttgtaatat ttgcgattgt attggttgga attgcaattc ttttgggagg
     1381 aagcttctat aacagccgta attttgtatc gatgggtttt caaattcctg agttcggatt
     1441 tttagcctta gccatgtcct tggcaatgtt aactggtggt atcgacttat ccattattgg
     1501 gaacatgaac ctttcgggaa tattagctgc cttaattctt accaaccaaa gcttgctttc
     1561 tacagccgga acgacagtga cggttgtatt ggcaatatta gtagcactct tatttggttt
     1621 aatttgtggt gcattaaatg gaattctgat tggatatata agaatacctg ctatgttggc
     1681 aacaataggt accatgttgt tttatactgg tgttggtatg gcaattaccg gaggacgagg
     1741 agtggtaggc tttccagaaa tttttatgtt gtttggaaat tctcgatttt taggattacc
     1801 aattcctctc cttattatgt ttattatttt tgccttggtc gccttggctc ttgaacacac
     1861 cgtatgggga cggagtgttt atttggttgg aaataactat atagcagcgc ttttttccgg
     1921 tgttcgaacc actaaaacta ttatcctgac ttacatgatt ggtgggttaa tgtgtggttt
     1981 tagtgccctg attcttattt cgagagtgaa ttcagcgcgg attggttatg gtgataccta
     2041 tcttcttcag gctattctgg tttcagtgat gggaggaatt aatcctgagg gtggttcagg
     2101 gaaagtgctt ggagtattgc tgagtatttt aatccttcaa tctctttcca gctctttcac
     2161 actttttgcc ataactccct atgcgagggg tttaatctat ggtttgatgt tgttgatcat
     2221 catgattgtc aattatgtat atggagaagg atccaaaaga tcgctacgaa aagccaaaat
     2281 aaaacaataa caaagaaata ccaagtggga ttctactgat ttagtaacct taagtgtatt
     2341 cgagaagtta taaggaggaa acaccatgca agacggagaa ttaattcaaa ttggatattt
     2401 agtaaaggac ttagaaggtg ccgtaaattt ttatatggaa accatgaatg ttggtccctg
     2461 ggaaatatat acttttgctc ctccagtgct tcgtgaatcg atggtgcgag gaaaaccctc
     2521 caatcatgaa tttcgcattg ctttagcctc accaggtcag attcaggttg aactcattca
     2581 acccttaact ggtcggagta tttatgatga atatctggaa caaaagggag agggattaca
     2641 tcacgtcaag tattattata aggattgtca aaaagtaatt caggaatttg agaaaaaagg
     2701 aattcccgtt attcaaagtg gaaagtttga tgaagacgaa tattattacc tggatacttt
     2761 aaagcactat ggaatgatga ttgaaatcgg gaacggagga aagattagac ctcccgaaag
     2821 gattatttca aaataaattg atttaaattt ttttaaatga aaaagtaaaa agatgaaatc
     2881 ctcataactt ctgaatacga aacccctgga tgactcat
//
LOCUS       DAOSOL010000179         2898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_34363_length_2898_cov_4.037636, whole genome shotgun sequence.
ACCESSION   DAOSOL010000179 DAOSOL010000000
VERSION     DAOSOL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34363_length_2898_cov_4.037636"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="PK171_04920"
     CDS             <1..1561
                     /locus_tag="PK171_04920"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05563.1"
                     /translation="KNEELAMDTILECRGIEKSFGGVHALKKVNFSLNPGEVHALVGE
                     NGAGKSTLIKIISGALSQDRGEIFYLEKAVNIGSPRKAQEIGISTVYQEPLIYRELSV
                     LENIFLGREIKTRRGNIDWNEEEKKARSLFESLQISPHFIKQPMRDLSVGLQQLALIA
                     KALVYEAKVIIFDEPTAILTEHETERLFEIIKKLKERKVGIIYISHRLEEIFRIADRV
                     TIMRDGEVVGEHLVGGINRSKIVELMAGRLLVEEINHLGKKGEKPILTVKNLTRKGRY
                     YDISFDIYPGEILGFSGLVGSGRTDIAQTLFGLIKPDSGEIVYQGKKISFSSSEEAMA
                     NGIAYLPEDRKAQGLFPILSTAFNISVTVLKEISSRMGIVHNSQEKELGKKYVKELAI
                     KTPSLDSKIYSLSGGNQQKVVLAKWLGVNPQILMLDEPTRGVDVASKSEIHHMIASLA
                     EKGIAVIIISSELPEILKLADRVIVMHEGRITGAFEDQDITSENLIRAATGERMLKTK
                     SSPIEEKNYDC"
     gene            1551..2501
                     /locus_tag="PK171_04925"
     CDS             1551..2501
                     /locus_tag="PK171_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05564.1"
                     /translation="MTANLQIVIKRREFTVLGLIVVLMVIFSLSTEKFMSVSNLSNLL
                     LQLSGVAISAIGMTMVIITGGIDVSVGSILGMASVVAGKLLMAGSSFVVVVLSAIITG
                     FGIGILNGIIISYGNVPPIIVTLGMMSMMRALMYQVLGGRWVSGIPPDIRIIGLGKWL
                     GIPLSMWITAVLIILFSYFLSSRPTGRAIYAIGNNPEAARVSGVNLARTILIVYSLTG
                     LLVGFAGLIYVARTGIVQTNTGSGFELEVIAAVMLGGTSVFGGKGTVIGSLLGAVLVG
                     IIKNGMVLMNVPALSEGLVIGILILISVMIDLVRSRGESL"
     gene            2498..>2898
                     /locus_tag="PK171_04930"
     CDS             2498..>2898
                     /locus_tag="PK171_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05565.1"
                     /translation="MKNIGSQIVENRVLFLLVLFLILFGVFAFSVKGFFTFYNLTNMT
                     QYGVELGLLALAEMLVILSGGAGIDLSVGSMLTIVAMIIGVLIGRAGFNPSLAILIGL
                     IAGSAMGYANGFLVAYPKIPPFIVTLASLYV"
BASE COUNT          796 a          566 c          659 g          877 t
ORIGIN      
        1 taagaatgag gagctcgcca tggataccat tctcgaatgt cgtggaatag aaaaatcttt
       61 tggaggagtt catgccttaa aaaaagtcaa tttttcttta aatcctggag aagttcatgc
      121 tttggttgga gaaaatggag ccggaaaatc aactctcatc aagatcattt ctggagcact
      181 ttctcaggat cgaggcgaaa tcttctatct tgagaaagcg gttaatattg gttccccaag
      241 gaaagctcaa gaaattggca tatctactgt ctatcaagaa ccactcattt atagggaatt
      301 gagtgtttta gaaaatattt ttttaggccg ggaaataaaa acccgaaggg gcaatatcga
      361 ttggaatgaa gaagaaaaaa aagctcgatc gttatttgaa tccctccaaa tatcccctca
      421 ttttattaag caacccatgc gagacctcag tgttggattg caacaattag cattaattgc
      481 caaagctttg gtatatgaag ccaaggtgat tatctttgat gaaccaaccg ccattttgac
      541 cgaacatgaa accgaaagac tttttgaaat tattaagaaa ttaaaggaga gaaaggttgg
      601 cattatctat atcagtcatc gcttagaaga aatattccgc attgctgatc gagttactat
      661 tatgcgagat ggtgaagtcg ttggtgaaca tctcgtcggt ggaatcaatc gcagtaaaat
      721 agttgaactc atggctggac gattgttagt ggaagaaata aatcatctgg ggaaaaaagg
      781 agaaaaacca atactcaccg tcaagaatct aacccggaaa ggacgttatt atgatattag
      841 ttttgatatt tatcctggag aaattttagg tttttctggg ttggttggct ccgggagaac
      901 tgacatcgct caaactcttt tcggactgat taaacccgac agcggtgaga tcgtttacca
      961 aggaaaaaag ataagctttt cttcttctga agaggccatg gccaatggaa ttgcctatct
     1021 ccctgaagat cgaaaggctc aaggcctttt ccctatttta agtaccgctt ttaatattag
     1081 tgtaacggtt ctaaaagaaa tttcttcaag aatgggtatt gttcataact cccaagaaaa
     1141 agagctcgga aaaaagtatg tgaaagagct tgccatcaag acacccagcc ttgattcaaa
     1201 aatctatagc ctcagcgggg ggaatcagca aaaagtagtc ttagccaagt ggttgggggt
     1261 aaatccacaa attctcatgc tcgatgaacc cacccgggga gtggatgtcg cctccaaatc
     1321 agaaatccac catatgattg cttctttagc tgagaaaggg atcgccgtga tcatcatctc
     1381 cagtgagctt cctgaaatcc taaaactggc cgatcgagtg attgttatgc atgaaggtcg
     1441 tatcactgga gcctttgaag atcaagatat tacctcagag aatttgattc gtgccgcaac
     1501 cggagaaaga atgttgaaaa ccaagagctc accaattgag gaaaaaaact atgactgcta
     1561 acctccaaat tgtaattaaa cgaagagaat ttacagtttt gggtcttata gtggtattaa
     1621 tggtcatttt ttcactgagt actgagaaat ttatgtcagt cagtaacctt tctaatttac
     1681 tcttacagct ttctggagtc gccatatcgg cgattggaat gaccatggtt atcattaccg
     1741 gtggtattga tgtttcggtg ggttcaatct taggtatggc ttcagtggtt gccggaaaac
     1801 ttcttatggc tggttcatcc tttgttgtcg ttgttctttc ggcaattatc accggttttg
     1861 ggattggcat cctcaatggg atcatcatct cttatgggaa tgtcccccct ataattgtca
     1921 ctttgggaat gatgagtatg atgcgagctt taatgtacca agttcttggt ggccgttggg
     1981 tttcaggaat tccacctgac atacggatca ttggcttagg aaaatggctg ggtatcccat
     2041 tatcgatgtg gattaccgct gtcttgatta ttcttttcag ctattttctc agttcccgac
     2101 caaccggacg ggcaatctat gccattggga acaacccaga agcagccaga gtttctggag
     2161 tcaaccttgc aagaaccatt ttaattgtct actcacttac cgggcttttg gtcggttttg
     2221 ccggactcat ctacgttgcc cgtaccggaa ttgtacaaac caatactggt tctggtttcg
     2281 aactcgaagt catcgcagct gtaatgttgg gtggcaccag cgtcttcggg gggaaaggaa
     2341 ccgtgattgg aagcttgctg ggagcagtct tggtaggaat tattaaaaat ggtatggttc
     2401 ttatgaacgt tcctgctctc agcgaaggct tggttatcgg gatactgatt ttgatttcgg
     2461 ttatgatcga cctcgtccga tccaggggag aatcgttatg aaaaatattg gaagccaaat
     2521 cgttgaaaac cgggtgctct ttctattggt attatttctc attctcttcg gggtgtttgc
     2581 cttttccgtc aagggttttt tcactttcta taacctcacc aatatgaccc aatacggtgt
     2641 ggaactaggg ttattggctt tagcggaaat gctggtcatc ctatcgggag gtgctggcat
     2701 tgacctctcg gttggttcca tgcttaccat tgtcgctatg attattggag tgcttattgg
     2761 ccgagctggt tttaatccat ctttagccat cctcattggt cttatcgccg gcagtgcgat
     2821 gggatatgcc aatggtttct tggttgctta tcccaagatc cctcctttta tagtcactct
     2881 tgccagcctt tacgtcta
//
LOCUS       DAOSOL010000180         2888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_34527_length_2888_cov_3.252736, whole genome shotgun sequence.
ACCESSION   DAOSOL010000180 DAOSOL010000000
VERSION     DAOSOL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34527_length_2888_cov_3.252736"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..2155
                     /locus_tag="PK171_04935"
     CDS             305..2155
                     /locus_tag="PK171_04935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT05566.1"
                     /translation="MNKKIKNNIKVTCLPMENFFRNPEKTAFKLSPDGKYLSYLQPWK
                     NRLNIYVEQIADGSIVQITQVTERDIAGYFWANNSRIAYLKDTGGDEDFKLYAVNIDG
                     TELKELTPFPGVRTELIDDLKDNEKEIIIGLNKRNPQFFDAYRVNVYTGEMDLIAENP
                     GNISGWMTDHEGRLRVAVTTDGVNSSLLYRDQEGQPFRSLITTNFRDTIAPLQFTYDN
                     QRLYVSSNLGRDLQAIYEYDPQLGKEVKLIYENSEVDVSVLLTSDKNKKITGVAYVTD
                     KLHYYFFDREREEIQQFLEKKLPGYEISIASMNREEDKILVRTYSDKSRGAYYYYDLH
                     NGEFKKIVEVSPWLTEEFLADMEPITFSARDGMVIHGYLTLPRTDDSKHLSVVVNPHG
                     GPWARDVWGFNPEVQFLANRGYAIFQVNFRGSTGYGRKFWEAGFKQWGRKMQDDITDG
                     VKWLIQKGIADPKRIAIYGGSYGGYAVLAGLTFTPDLYAAGIDYVGVSNIFTLLETIP
                     PYWEQGRQMLYEMIGDPEKDRDLLQSASPVFHADKIRAPLLVAQGANDPRVKKAESDQ
                     IVEALRHREVDVKYIVKENEGHGFRNEENRFDFYREMEQFLAKHLDGMIC"
     gene            2201..2713
                     /locus_tag="PK171_04940"
     CDS             2201..2713
                     /locus_tag="PK171_04940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT05567.1"
                     /translation="MKTIIKLLWLVLLFIIPLGSFSFVWSQDQTQLEDFQLSDFTLMT
                     LDGQEITLSKLTGKPVLLTFFLTTCSHCQAESASLNKVFNKYHVSKELQILAVAPRID
                     SRENLVKFIDEYQITYPILHDQENQVLKLYGVIAVPHNIFIDREGKVKRVITGEVDLT
                     TLEESLEEIW"
BASE COUNT          958 a          487 c          607 g          836 t
ORIGIN      
        1 atctcatctt tttgaaaggc cgagggggcg agggggagaa tgggagaggg ggagaaaaaa
       61 aattaaagaa taaaagaaaa aataaaaaga aaagatactc acgccctcaa aaagcgaggg
      121 ctcaggatga cggattaaag acaccctccc cgccgtaagg cgtcatccct ccaaggaggg
      181 gaattataag atggtatttt cagaatagaa acatgaaggt tgaaaggata atagatattg
      241 aattataata accgtctatg cctttttaaa agcttttctt ttttctgagg aggactgaaa
      301 aataatgaat aaaaagatca aaaataatat caaagtaact tgtttaccga tggaaaattt
      361 ctttcggaac ccggagaaaa cggctttcaa actttctccc gatggaaaat atctatctta
      421 ccttcaaccc tggaaaaata gattgaatat ttatgttgaa caaatcgccg acgggtcgat
      481 tgtccaaata actcaagtta ccgaacgtga tattgctggc tatttttggg caaataattc
      541 tcgaatagcc tatttaaaag atactggtgg ggatgaagat tttaaattat atgcggttaa
      601 tatagatgga accgaattaa aagaattgac gccctttcca ggagtacgta ctgaattaat
      661 tgatgattta aaagataacg agaaagaaat tattattggt cttaacaaaa ggaatcctca
      721 attttttgat gcttatcgag ttaatgttta tactggagaa atggacctca ttgcagaaaa
      781 tcctggaaat atttctggtt ggatgaccga tcatgaaggt cgattacgag ttgcggtgac
      841 gaccgatgga gtgaactcca gccttctcta tcgggatcag gaaggccaac catttcggag
      901 cttaattacc accaatttcc gcgataccat tgctccgctt caatttacct atgataatca
      961 acgattatat gtttcttcta atttaggtcg agatttgcaa gctatatatg aatatgaccc
     1021 acagttgggg aaagaagtaa aactgattta tgaaaattcg gaagtggatg tatcggttct
     1081 tttgacatca gataaaaata aaaaaataac cggtgtagcc tatgttaccg ataagctaca
     1141 ttattatttt tttgatcgag aacgggaaga aatccaacaa tttcttgaaa aaaaactccc
     1201 tggatatgaa atttcaatag ccagcatgaa ccgggaagaa gataagatcc tggttagaac
     1261 ttatagcgat aaatcacgag gtgcttacta ttactatgat cttcataatg gagaattcaa
     1321 aaaaatagtt gaagtgagtc cctggctaac ggaagaattt ttagccgata tggaaccaat
     1381 aaccttttcc gccagagatg gtatggttat tcatggatat ctgactctcc ctcgaactga
     1441 tgattccaaa cacttatcag ttgtcgtaaa tccccatgga ggtccttggg caagagatgt
     1501 ctggggcttt aatccggaag tccaattttt agccaatcgt gggtatgcga tttttcaagt
     1561 taattttcgt ggatcaactg ggtatgggag aaaattttgg gaagccggtt ttaaacaatg
     1621 gggtagaaag atgcaggatg acattactga tggagtcaaa tggttgattc agaaaggaat
     1681 tgctgatccc aaaaggattg ccatttacgg gggttcttat ggtggatatg cagtattagc
     1741 tggtttaacc tttactcccg atttgtatgc agccggaatt gattatgtgg gagtttccaa
     1801 tatatttacc cttttagaaa ccattcctcc ctactgggag caagggcgtc aaatgcttta
     1861 cgaaatgatt ggcgatccgg aaaaagatcg tgatttactc cagtcagcat caccagtgtt
     1921 ccatgcggat aaaattcgag cacccttgct ggttgctcag ggggcgaacg atccacgagt
     1981 taaaaaagcg gaatcagatc aaattgttga agctcttcgc catagagagg tagacgttaa
     2041 gtatatagtt aaagaaaatg agggccatgg ttttcgaaat gaagaaaacc ggtttgattt
     2101 ttaccgagag atggaacaat tcttagccaa gcacttggac ggaatgatat gttagtaaaa
     2161 tggttatatt acttaaaatg taaaaaggag tgtgttgaag atgaaaacaa taatcaagct
     2221 cctttggttg gttttacttt ttatcattcc gttggggagt ttttcatttg tttggtctca
     2281 agatcaaacc caattagaag attttcagct cagtgacttt acactgatga cattggatgg
     2341 tcaagaaatt actttaagta aattaaccgg aaaaccagtt ttgttaacat ttttcttgac
     2401 aacctgttct cactgccaag ctgaaagcgc atccttaaat aaagtcttta ataaatacca
     2461 tgtatcaaaa gagcttcaaa ttttagcggt tgcaccacgc atagatagca gggagaatct
     2521 ggtgaagttt attgatgaat atcaaataac ttatccaatt cttcacgacc aagagaatca
     2581 ggtgttaaaa ctttacggag taattgcggt tccccataat atatttattg accgagaagg
     2641 aaaggttaaa agagtcatta ccggtgaggt tgatctgacg actttggaag aatctcttga
     2701 ggaaatatgg taaaaagaag tgcggatatc aacaaaccta tttgcatttt tgccatcaaa
     2761 cggaaattcc gcatctccac ccctcccata acatctcaag agattatccc gtaagaagaa
     2821 aatgcaaaaa atcagtttct cggtatccgc accaactata ttataccata tttttatttt
     2881 ttcaataa
//
LOCUS       DAOSOL010000181         2879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_34660_length_2879_cov_4.394122, whole genome shotgun sequence.
ACCESSION   DAOSOL010000181 DAOSOL010000000
VERSION     DAOSOL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34660_length_2879_cov_4.394122"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PK171_04945"
     CDS             <1..381
                     /locus_tag="PK171_04945"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOT05568.1"
                     /translation="DMPPLKAFLEGKDIDLREKLNLFTPEEAAEQYQIRQHRIQEIKS
                     KLMEWGYWRETDTFEDLLEAILLYLSKTSSTLKIVNLDDLIESDVQLNIPGTINEYPN
                     WRHRLLVGCDELESKIQKMALIFK"
     gene            398..1504
                     /locus_tag="PK171_04950"
     CDS             398..1504
                     /locus_tag="PK171_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05569.1"
                     /translation="MRIIEFIQKKEAQPDFQQFIEVVTKKRKPAKVHQVELLIDQEII
                     SAISQKYFLEEMVPLTSQTQRQYIQQYVNWWYRMGYDYTMIIDIPGIGLEFPSQMRKT
                     DDTAYLSRGARNWVEEKKGIIANWENFENYPWPDPEDIDLSPYLLAQEEMPEGMKLLV
                     AGSSGLLEVVSENLLGIENMSYLLFDEPDLVEAVFEKVGNIMYRFYLRLSDLEGIGGF
                     FQGDDMGFKTSTMLSPAFLKKYVLPWHKKVCELAHLKGKIYFLHSCGQLKPIMDDLIN
                     WVGIDAFHSFQEEVISVVKYKKEYGSRTGVLGGVDLDKLVRCNELELRNYVRLILQKC
                     QNTGGYALGSGNSIANYVPINNYLIMLDEGLVYE"
     gene            complement(1522..2313)
                     /locus_tag="PK171_04955"
     CDS             complement(1522..2313)
                     /locus_tag="PK171_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT05570.1"
                     /translation="MNQPKVACVQFKPVLGNVKRNLETISNFIEKGKELKVDIMVFPE
                     LCVQGYDPKLVASKAEAQGGNSFQLLSEASKFSGIYLVVGIAERLKDKLFNSSVLFYP
                     DGKIGVYRKVHLWSTEAEIFTHGSDYPVFDTEFGKIAMWICYDTRFPEVARILALKGA
                     RIALVPTAWLARDIEHWKLSVCSRALDNFMYVCGADEILQTEYHQSHGASIIVNPHGQ
                     IISQANLMEEMVISSTIDLSSVDELRHLIPVFEDRQKSTYNRLYT"
     gene            2612..2687
                     /locus_tag="PK171_04960"
     tRNA            2612..2687
                     /locus_tag="PK171_04960"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Thr,seq:tgt)
     gene            2693..2779
                     /locus_tag="PK171_04965"
     tRNA            2693..2779
                     /locus_tag="PK171_04965"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2727..2729,aa:Tyr,seq:gta)
     gene            2795..2869
                     /locus_tag="PK171_04970"
     tRNA            2795..2869
                     /locus_tag="PK171_04970"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2827..2829,aa:Thr,seq:ggt)
BASE COUNT          884 a          494 c          609 g          892 t
ORIGIN      
        1 gatatgcctc ccttgaaagc atttttagaa gggaaagata tcgatttaag agaaaaatta
       61 aatttattta ctccggagga agcggctgaa caatatcaga ttcgccaaca tagaatacaa
      121 gaaataaaaa gtaagctaat ggagtggggg tattggcggg aaactgatac ctttgaggat
      181 ttacttgaag ctatccttct ctatctatct aaaacttctt cgacattgaa aattgtcaat
      241 ttggatgatt taatagaaag tgatgttcaa ctgaatatac cgggaaccat taacgaatat
      301 ccgaattggc gtcaccggtt attggttggt tgtgatgaat tagaaagtaa aatccaaaaa
      361 atggcattaa tttttaaata ataaaggaaa agttttcatg agaattattg agtttattca
      421 aaagaaagaa gctcagccgg attttcaaca atttatcgag gttgttacca agaaaagaaa
      481 accggctaaa gttcatcaag ttgaactatt gattgatcag gaaattatct cggcaatcag
      541 tcagaaatat tttttagaag aaatggtacc actcacttct caaactcaaa ggcagtatat
      601 ccaacaatat gttaactggt ggtatcgaat gggctatgat tacaccatga ttatcgatat
      661 acctggaatt ggtttggagt ttccatcaca gatgagaaaa accgatgata cagcttattt
      721 gtcgagggga gccaggaact gggtagaaga gaagaaaggg attatagcca attgggagaa
      781 ttttgaaaac tacccctggc cagatcctga agatattgat ttgtcacctt atcttcttgc
      841 tcaagaagaa atgcctgaag ggatgaagct tttagttgcc ggaagtagtg gattgttaga
      901 agtagtgagt gaaaatttat tgggaattga gaatatgagt tatttgttat tcgatgagcc
      961 ggatttggtc gaggcggtat ttgaaaaagt gggcaatatt atgtatcgtt tttatcttcg
     1021 actttccgac ttagaaggga ttggtggatt ttttcaaggt gatgatatgg gatttaaaac
     1081 ttcaaccatg ctatctccgg cttttttaaa aaaatacgtc cttccctggc ataaaaaagt
     1141 ttgtgaattg gctcatttga aagggaaaat ctacttcctt cattcctgtg ggcaattaaa
     1201 acccattatg gatgacctta ttaactgggt gggcattgac gcttttcatt cgtttcagga
     1261 agaagtaata tcagtggtta aatataaaaa ggaatatgga tcgcgaactg gtgtcctcgg
     1321 cggcgtggat ttggacaaat tagttcgatg caatgaattg gagctccgta actatgttcg
     1381 attgatcctg caaaagtgtc aaaatacggg aggttacgct ctgggttcag gaaattcgat
     1441 agctaattat gttcctataa acaattatct tattatgctt gatgaaggat tggtttacga
     1501 ataggtgtat agtttattat tttaagtgta caggcggtta taagtacttt tttgccgatc
     1561 ctcaaaaacc ggtatcagat gccgtaattc atctacgcta cttaggtcga tagttgagga
     1621 tataaccatt tcctccatga gattggcttg gctgatgatt tgtccgtgag gattaacgat
     1681 tatacttgct ccatgggatt gatggtattc agtttggaga atttcgtcag ctccacaaac
     1741 atacataaag ttatccaaag cccgtgaaca aactgataat ttccagtgtt cgatatctcg
     1801 tgccaaccaa gcggtaggga ccagagcaat acgagctcct ttaagagcta aaattcgagc
     1861 aacttcgggg aaacgagtat cgtaacagat ccacattgct attttgccga attctgtatc
     1921 aaaaactgga taatcactac catgagtaaa aatttcagcc tctgttgacc aaaggtggac
     1981 ttttcgatat actccgattt ttccatcggg ataaaacaaa actgaagagt tgaatagttt
     2041 atcttttaat ctttcggcaa taccgacaac caaataaatt ccggaaaatt tactggcttc
     2101 gctgaggagt tgaaatgagt tgccaccctg tgcttctgct ttggatgcta ctaatttagg
     2161 atcatatccc tgaacacaaa gctctggaaa aaccataata tcaactttga gttcttttcc
     2221 tttttcgata aaattgctaa tagtttctaa attcctttta acgttaccca ataccggttt
     2281 aaattgaaca caagctacct taggctggtt catagaaatc ccctttccgg taatattatt
     2341 caaattatta tatcatttaa tgaatattgt tttaaggttg tataataata aaaaaagaac
     2401 attaagggat ttatctttaa aattttttct aataattatc agcttgagca ggtgggtttc
     2461 ttggtcgatg ttgcacttat caattgttta agtatcaaag ctgttgcagg gcttatcttt
     2521 ataaaaataa atagattttt tgtttcttac ccttgacatc aactcaacct ttggatatat
     2581 ttattcaggc ataaccggaa gcagccggtt tgccggtgta gctcagctgg tagagcagct
     2641 gacttgtaat cagcaggtcg tccgttcgag tcggatcacc ggctccagaa atggggaggt
     2701 acctaagtgg ccaaaaggag cggactgtag atccgccggc tgaacgcctt cgaaggttca
     2761 aatccttccc tccccaccag aaaatcccta tgtagcctgc gtagctcagt ggtggagcgc
     2821 atccttggta aggatgaggt cgccggttca atcccggccg taggctccat tttttattt
//
LOCUS       DAOSOL010000182         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_34688_length_2878_cov_3.357775, whole genome shotgun sequence.
ACCESSION   DAOSOL010000182 DAOSOL010000000
VERSION     DAOSOL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34688_length_2878_cov_3.357775"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PK171_04975"
     CDS             <1..282
                     /locus_tag="PK171_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT05571.1"
                     /translation="RGILTPVEITVYGDRSFTFVTKTPPASFLILQALGIEKGSGEPN
                     KVKVGKLTRSKVREIAQKKMPDLNANSIEAAMNIIEGTARSLGVEVVEG"
     gene            302..1018
                     /gene="rplA"
                     /locus_tag="PK171_04980"
     CDS             302..1018
                     /gene="rplA"
                     /locus_tag="PK171_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT05572.1"
                     /translation="MPTKSRRFEELKNKLEPGKIYTVDEAFQMVKELANAKFDETIEV
                     AVKLGIDPKQSDQQIRGTVSLPHGIGKQVRVLVFALGEKASEAQAAGADYVGGEDLVE
                     KIQSGWFDFESAIATPDMMRVVGRLGKILGPKGLMPNAKVGTVTFDLAQAVKEIKAGR
                     LEIRNDRYGNIHLPIGKASFEISKLQDNFFALLETIVRMKPSAAKGRYVRNIAISSTM
                     SPGLKLDVNNTLARMEKRVA"
     gene            1154..1678
                     /gene="rplJ"
                     /locus_tag="PK171_04985"
     CDS             1154..1678
                     /gene="rplJ"
                     /locus_tag="PK171_04985"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT05573.1"
                     /translation="MDKIKKNQIIEDILQKLQKSQAVYAFNYRGINVMNVEAIRKALR
                     DSQGEMKAVKNTLAHIAFQKADIPFDESILTDQNALAFSYQDIVLTAKTLTDFAKKVP
                     QLEIKGVWLNQQQYGPEQVKNLASLPTREVLVAQVVGGIAAPLSGLVGALSGVLRNMV
                     WVLKSIEEKKVSNQ"
     gene            1694..2068
                     /gene="rplL"
                     /locus_tag="PK171_04990"
     CDS             1694..2068
                     /gene="rplL"
                     /locus_tag="PK171_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT05574.1"
                     /translation="MTKEEIIDVIANMTVLELADLVKALEEKFGVSAAMPVAAATAPN
                     AGAAAPVEEEKTEFTVVLKSAGAEKLKVIKEVRAITSLGLKEAKDLVDGAPKPVKEAI
                     PKKEAEEIKAKLEAVGAEVELT"
     gene            2159..>2878
                     /locus_tag="PK171_04995"
     CDS             2159..>2878
                     /locus_tag="PK171_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT05575.1"
                     /translation="MRNSENYIPTSSSHPEEKSEPLVKNPYISLLMVSLIWGINFSIV
                     KQGVMIIGPLSFSLIRFTVSSLIMIILLWRIEGNPFIKKKDIWYFIFLGALGFGIYQP
                     LWSYGLRLTLASHSAILLSISPIVVVLIAYFKKEENVEWYNFLGIGIGFLGVTFLISQ
                     GKPSSFSPHILLGDILTLLAAICWGLYSYFGKHILRKYSPLKTSCWSIIFGTFIMFPI
                     SYFEIIDLDLSDMTLPVYLSMG"
BASE COUNT          910 a          464 c          644 g          860 t
ORIGIN      
        1 cgggggattt taacaccggt agaaataact gtatatggtg atcgttcatt tactttcgta
       61 acaaaaaccc ctccggcttc tttcttgatt cttcaagctt tgggtattga aaagggctct
      121 ggggaaccaa acaaggtcaa agttggaaaa ttaactcgtt ctaaggtacg agaaatagcc
      181 cagaagaaaa tgcctgattt aaatgcgaat tcaatcgaag cagccatgaa tataattgaa
      241 ggaaccgcaa gaagcttggg tgttgaggtc gtagagggtt agggagagtg aggtgagaag
      301 tatgccaact aaaagtcgac gttttgaaga attaaaaaat aaattggaac caggtaagat
      361 ttatactgtt gatgaagcat ttcaaatggt taaagagtta gcaaatgcga aatttgatga
      421 aaccattgaa gttgccgtta agctgggaat cgatccaaaa caatcagacc agcaaattcg
      481 tggtacggtt tccttgcccc atgggatagg aaagcaagtc cgggttttgg tttttgctct
      541 gggggaaaaa gccagtgagg cacaagctgc tggtgctgat tatgttggtg gagaagattt
      601 agtagagaaa atccaatcgg gttggtttga ttttgaatca gctattgcca ctccagatat
      661 gatgagagta gtaggtcgat taggaaaaat attgggacca aaagggttaa tgcccaatgc
      721 caaggtagga accgtaacct ttgatctagc tcaagcggtt aaggaaataa aagccggtcg
      781 attggaaata cgaaatgacc gttatggaaa tattcatctt ccaatcggga aagcttcttt
      841 tgaaataagt aaattacaag ataatttctt tgcactatta gaaacaatag ttcgaatgaa
      901 accttcagca gctaagggtc gttatgtaag aaatattgct atttcttcaa ccatgagccc
      961 tgggctcaag ttggatgtga acaacacttt agcaagaatg gagaaaaggg tcgcttaaaa
     1021 taaaccgaaa ttgaatataa ttatgcccat gaaccggggg cggtcgctta aatatcctgg
     1081 ggaggcttta ttgatatgaa gctctcgtat ttatgggagc ttttttattt cggaagaaag
     1141 gaggttgtta tttttggata agataaaaaa gaaccagata attgaagaca ttttacaaaa
     1201 actccaaaaa agtcaagcag tttatgcttt taattatcgt gggattaatg ttatgaatgt
     1261 tgaagccatc cgaaaagctt tacgagatag tcaaggagag atgaaagctg ttaaaaatac
     1321 ccttgctcat attgcttttc aaaaggccga cattcccttt gatgaaagta ttttgactga
     1381 ccaaaatgcg ctggcttttt cttatcagga tatagtttta acggccaaga cactaacgga
     1441 ttttgccaaa aaagtgcctc agcttgaaat taaaggggta tggctcaacc aacaacaata
     1501 tggaccggaa caagtgaaaa acctggcaag tttaccaacc cgagaggttt tggtcgctca
     1561 ggtagtaggt ggaatcgctg caccgcttag cgggttagtt ggtgctctaa gcggagtttt
     1621 gcgaaatatg gtatgggttc ttaaatcaat agaagaaaaa aaggtaagca atcaataagg
     1681 gaggtaccta taaatgacca aggaagaaat tattgacgtt attgcgaata tgactgttct
     1741 ggaattagca gatttggtaa aggccttaga ggaaaaattt ggagtttcag cagctatgcc
     1801 agttgctgca gcaactgctc caaatgccgg agcagctgca ccggttgaag aggaaaaaac
     1861 tgaatttaca gtcgttctca agagtgctgg agctgaaaaa ttaaaagtta tcaaagaagt
     1921 tcgagccatt actagtttag gtttgaaaga agccaaagac ttggttgacg gagctccaaa
     1981 accagtgaag gaagctattc caaagaagga agccgaagag attaaagcca aactggaagc
     2041 tgtcggtgct gaggttgaat taacctagtg attgaatttc cgatgtgaag ggacccaaat
     2101 gaaagggtcc ctttttcttt tttatccaaa aatatcataa tcagaaaaga gtaaatcaat
     2161 gaggaattcc gaaaactaca tcccaacatc atcttctcac cctgaagaga aatccgaacc
     2221 cttggttaaa aatccttata tttctcttct tatggtttcc ctgatttggg gaataaattt
     2281 tagtattgtg aaacaggggg ttatgatcat tggtcctctt tctttttcct taattcggtt
     2341 taccgtgagt agtttgatta tgataatttt attatggcgc attgaaggga acccttttat
     2401 caaaaagaaa gatatctggt attttatctt tttaggtgct ttgggttttg gaatctatca
     2461 accactatgg agttatggtc ttcgattaac tttagcatcc cattcggcta ttctgctctc
     2521 aatatctcca attgtagttg ttcttattgc ttattttaaa aaagaagaaa atgtcgaatg
     2581 gtataatttt ttgggaattg gcattggatt tttaggtgtt accttcctga ttagtcaagg
     2641 aaaaccaagt tcattttctc cccatatact attaggagat attcttactc ttttggcagc
     2701 tatttgttgg ggtctttatt cctattttgg gaagcatata ctacgtaaat attcaccatt
     2761 gaaaaccagc tgttggagta tcatatttgg aacttttatt atgtttccca tctcatattt
     2821 tgaaattatt gacctcgatc tttctgatat gactctaccg gtttatttgt cgatgggg
//
LOCUS       DAOSOL010000183         2874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_34751_length_2874_cov_3.818375, whole genome shotgun sequence.
ACCESSION   DAOSOL010000183 DAOSOL010000000
VERSION     DAOSOL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34751_length_2874_cov_3.818375"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1228)
                     /gene="galT"
                     /locus_tag="PK171_05000"
     CDS             complement(149..1228)
                     /gene="galT"
                     /locus_tag="PK171_05000"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HOT05576.1"
                     /translation="MQKKGYSRKDKEFEEVWRKVMSELRKDPVVDRWVIIANERSLKP
                     YDFGVHGEIRKPGPCVFCEGMEMHTPKEVFSVRDKHTEINQPGWRVRVVPNKFPALRI
                     EEPFAHCIDGICESLSGSGAHEVIIETPDHYLELGDLSVKHIAEVMYVYRERMCDLER
                     DHRFRYCIIFKNQGALAGASIQHAHSQLIAIPVIPKRVKEELLGADKYYQEKGHCIFC
                     DIVQYHQNNNQRMIIENEQFLAFVPYAPRFPYEIWILPKKHSSHFMKIEDQELNYLSE
                     ILKKLLVSMKKSLNDSPYNLILHAAPFERKEEKSYQDSYHWHMEMLPTITKVAGFEWG
                     TGFYINPVIPENAAEILRKNIPIKI"
     gene            complement(1254..2312)
                     /locus_tag="PK171_05005"
     CDS             complement(1254..2312)
                     /locus_tag="PK171_05005"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOT05577.1"
                     /translation="MEKSREEFLKKLIETPSPSGFEEQIQKIFEERVQGKVDKFYKDY
                     HGNAIGVIYPDASFRVMLAGHCDEVGLMVEFINDQGYIHFSTIGGIDSHVTPGKRVII
                     HSSQGPVKGVIGKKPIHLMEEKERQKVIKFEEQWIDVGAKNKEELTKIVQIGDPITMD
                     VGFDQLLGSRVVGRGFDDRVGAFIVAEVLRMVQKEKLQCGVYGVSTVQEELGLRGART
                     SAFRIDPHLGIAIDVTFASDCPDIDKRKVGDVSLGKGPVISRGPNINPHIFRKLVEVA
                     TQKNIPYQIQAESRATGTDANSIQINRQGVATGLLGIPNRYMHTPSEVIDCDDLDAAI
                     QLLAGFLENIHPEENWIP"
     gene            complement(2391..>2874)
                     /locus_tag="PK171_05010"
     CDS             complement(2391..>2874)
                     /locus_tag="PK171_05010"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOT05578.1"
                     /translation="MGDIMQESARIALSCVRERAKKWKIDEKFYEKHDIHVHVPAGAI
                     PKDGPSAGITLVVSMVSSLSQIPVKHSIAMTGEITLTGKVLPIGGVKEKSLAAYRSGI
                     KEVVLPQENENDLDDIPADVRKKMKYHLVKTIDEVLKITLKKSPRMVKKKKTSSLQEK
                     "
BASE COUNT          836 a          630 c          468 g          940 t
ORIGIN      
        1 ccctttccca ccggccaaaa tgagcgcaaa tcgaatcgac atctggctca cctcctatgc
       61 atcaccatca aaccattatg gatctgtgac caattgctga gaattcctat tattatacag
      121 ctgtaccctg aatttcaatt aaaaagaact aaatcttaat gggtatattc tttctgagaa
      181 tttctgctgc attttcggga attactggat taatataaaa cccagtaccc cattcaaatc
      241 cagctacctt ggttatagta ggcaacattt ccatatgcca atgatagcta tcctggtatg
      301 atttttcttc ttttcgctca aaaggagcag catgaagaat caaattatag ggagaatcat
      361 tcagcgattt tttcatagat accagtaatt ttttcaatat ttctgataaa taatttaatt
      421 cttggtcttc aattttcata aaatgagatg agtgtttttt tggtaatatc caaatttcat
      481 aaggaaatcg aggagcatag ggaacaaacg ctaaaaactg ttcgttttcg ataatcatcc
      541 tttgattatt attttggtgg tattgaacaa tatcacaaaa aatgcaatga cctttttctt
      601 gataatattt atcagcacca agcagttcct ctttcaccct tttgggaatc actggaatag
      661 caataagctg ggagtgagca tgttgaatcg aagcccccgc taaagctcct tgatttttaa
      721 agataatgca ataacgaaaa cgatggtctc tttccaaatc gcacattctt tcccgataaa
      781 catacataac ttcagcaata tgtttcaccg aaagatcacc aagctctaag taatgatcgg
      841 gagtttcaat aataacttca tgtgctcctg aaccactgag agattcacaa attccatcta
      901 tacaatgggc aaagggttct tcaatccgca aagcgggaaa tttattcggg accacccgaa
      961 ctctccaacc tggttgattt atctcggtat gcttatctcg aacggaaaaa acctccttcg
     1021 gagtgtgcat ttccatccct tcacaaaaaa cacaaggacc aggttttcgt atttctccat
     1081 gtactccaaa atcatagggt tttaaacttc tttcattggc aataatgacc caccggtcaa
     1141 caacaggatc ttttctcagt tccgacatga ctttcctcca aacttcttca aactctttat
     1201 cttttcgact gtatcctttt ttctgtatta actatccggt gatataaaaa tttttaaggt
     1261 atccaatttt cctcgggatg aatgttttct aagaatccgg ccaataattg aatggctgca
     1321 tccaaatcat cacaatcaat cacctcagaa ggagtatgca tatatcgatt gggaatgcct
     1381 aacaaaccgg tagcaactcc ttggcgattg atttgaatgc tgttggcgtc ggtccctgta
     1441 gcacgtgatt cagcttgtat ttggtaggga atgttttttt gggttgccac ctctactaat
     1501 tttcgaaaaa tatggggatt aatattcgga cctcgtgaaa tgaccgggcc cttgcccaaa
     1561 gacacgtctc caacctttct cttatctata tcaggacagt cagaagcaaa ggtcacatca
     1621 attgcgattc ctaaatgagg atcgattctg aaagcgctgg ttcttgctcc cctgagaccg
     1681 agttcttctt gtaccgtact cacaccatat acaccacatt gcaacttctc tttttgaacc
     1741 attcgaagaa cctcggcaac aataaaagct cccacccgat catcgaatcc tcgaccaaca
     1801 actcgacttc caagtaattg gtcaaaacca acatccatgg tgattggatc gccaatttgt
     1861 acaattttgg tgagttcttc cttatttttt gcgcccacgt caatccattg ctcttcaaat
     1921 ttaatcactt tttgacgttc tttttcttcc atcaaatgaa tgggcttttt tccgattact
     1981 cccttaactg gtccttgaga gctatgaata atgactcttt ttccaggggt aacatgagaa
     2041 tcgattcctc caatagtgga aaaatgtatg tacccctgat cattaataaa ttccaccatc
     2101 aagccgactt catcacaatg tcctgccaac ataactcgaa acgaagcatc aggataaata
     2161 acaccaatgg cattgccgtg gtaatctttg taaaatttgt caaccttacc ttgaaccctt
     2221 tcctcaaata ttttttgaat ctgttcttcg aaccccgatg gactaggagt ttcaatgagt
     2281 tttttcaaaa attcttctcg agatttctcc attttaatat tccctctttc tcaaacctgt
     2341 ttatattgaa taagaactaa aaaatgtttt tgtgctgatt caatcagcca ttatttttct
     2401 tgaagagaag aagttttttt ctttttaacc attcggggtg atttttttaa ggtaatttta
     2461 aggacttcat caattgtttt aactaaatga tatttcattt tttttctcac atctgccggt
     2521 atatcgtcta aatcattttc gttttcttga ggtagaacaa cttctttaat ccctgatcga
     2581 taggctgcta aagatttttc ttttactcca ccaataggaa gcacttttcc ggtaagggtt
     2641 atttcaccag tcatagcaat ggaatgcttt actggtattt gcgaaagaga agaaaccatt
     2701 gaaacaacca gggttattcc tgctgatgga ccatcttttg gtatagcgcc agcggggaca
     2761 tgaacatgaa tatcatgttt ttcgtaaaac ttctcatcaa tcttccactt tttcgctcgt
     2821 tcccgaacac aagatagtgc aatacgagcc gattcttgca taatatctcc cata
//
LOCUS       DAOSOL010000184         2863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_34929_length_2863_cov_3.189103, whole genome shotgun sequence.
ACCESSION   DAOSOL010000184 DAOSOL010000000
VERSION     DAOSOL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34929_length_2863_cov_3.189103"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK171_05015"
     CDS             <1..219
                     /locus_tag="PK171_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05579.1"
                     /translation="NLLNPEAILIGGELADLGDLIINPVKGTVRRQSLELATRNLAFK
                     VAEIKDRPASRGAASLVLNQVFKGNELF"
     gene            complement(318..1235)
                     /locus_tag="PK171_05020"
     CDS             complement(318..1235)
                     /locus_tag="PK171_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05580.1"
                     /translation="MVFQYGLSDFIPFKDVKECERVRKIKKGDITKHHNPDFKIKVIE
                     DPNQFYIEFALDLVSRIKKSAEMNDKLVLILPVGPVPQFEIAARLINEFNLSMKNVHT
                     FNMDEYADENGNTAPIDWPGSFQKAMWENFFNKIKPELRPDSKNIHFPTKDALPDYGK
                     MIEDLGGADCCYGGVGWCGHIAFWEAHLGFEFGNDLDAYKKQGPRCVELHPMTIMQNA
                     LHSFSGDWSWVPPKANTIGPAQIVGAKDRSFWLDGYLGGGVSWQRFIARLAAHGPVNT
                     LVPASLLQTAPGTYTILGGVADNVEIHMA"
     gene            complement(1404..2204)
                     /locus_tag="PK171_05025"
     CDS             complement(1404..2204)
                     /locus_tag="PK171_05025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOT05581.1"
                     /translation="MFKDRLNKALDIVKIAGMIAQERQQSIGHIEDKTSPMDVVTDID
                     RETQDLILKTLKKNFPEDITWGEESGYPLKDFSSTWVLDPIDGTTNYIHGLPLYSISL
                     AYYHQGIPVIGVIDSPAFGETFFSERGNGAYLNGNPIQLSDVKDLRRSLLSTGFPHEQ
                     NRCDIIIPVYKHLLCRTQALRAIGSAALGIAYVACGRFEAYFQFGISFYDVAAGVCIL
                     EEAGGRVLQINEKPWGTTSRSIVAINPLIEQQLFDELNRFQIPEPEYY"
     gene            complement(2552..>2863)
                     /locus_tag="PK171_05030"
     CDS             complement(2552..>2863)
                     /locus_tag="PK171_05030"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT05582.1"
                     /translation="LGGIGHVIGEQIEEKTGIETRVVILGHLLRGGSPTPYDRVLASR
                     FGAAAVDLVAQEQFGLFPALRGNEIIPVPIKKAIDKLKTIPLGHSLLQVARSTGTCLG
                     E"
BASE COUNT          865 a          642 c          536 g          820 t
ORIGIN      
        1 aacttactca accctgaagc tatactcatc ggtggggaac ttgccgatct cggtgacctg
       61 atcatcaacc cagtaaaggg aacagtacgt cgtcagtcct tagaattagc cactcggaat
      121 cttgctttta aagttgctga gatcaaagat cgaccggcgt ctcgtggagc ggcatctttg
      181 gttcttaatc aagtattcaa aggaaacgag cttttttgac tttcagtttg gttgaataaa
      241 acaaatacaa aaaaggcatt aaaaaagggc gctaatttat tgcgcccttt tttaaaatca
      301 acctggcaac ttatttttta agccatatgg atttcaacat tatcagcgac accacccagg
      361 atagtatagg tccctggagc agtttgtaaa agagaggctg gaactaaagt attaaccgga
      421 ccatgagctg ctaaacgggc aataaaacgc tgccaactga caccgcctcc gagatatcca
      481 tctaaccaga aactccgatc ttttgcacca acaatttggg caggaccaat tgtgttggct
      541 ttgggaggaa cccaagacca gtctccgcta aaggaatgca gagcattctg catgatggtc
      601 ataggatgga gttcaacaca acgaggtcct tgttttttat acgcatccaa gtcatttcca
      661 aattcaaatc ctaaatgagc ttcccaaaaa gcaatatgac cacaccaacc tacaccacca
      721 taacaacagt cagcaccacc taagtcctca atcattttcc cataatcagg aagggcatct
      781 ttggtaggaa agtggatatt ttttgaatct ggtcgtaatt ccggttttat cttgttaaaa
      841 aagttttccc acatggcttt ttgaaacgaa cctggccaat caattggagc agtattaccg
      901 ttttcatcag catactcgtc catattgaag gtatgcacat ttttcattga aaggttgaac
      961 tcattgatca aacgagcagc aatttcgaat tggggaacgg gaccaactgg aagaattaaa
     1021 actaacttat cattcatctc ggctgacttt ttgatccggc taaccaaatc caatgcaaat
     1081 tcaatataaa attgattggg atcctcaatg actttgatct taaaatctgg attgtggtgc
     1141 ttggttatat ctcccttttt gattttccta acccgttcac attcctttac atctttaaaa
     1201 ggaataaaat cagacaaacc atactgaaat accattttta tacctcctaa atattaattg
     1261 tttaatatcc tttaatctat aactttaagt attcacttta tttagggatg tcttacctcg
     1321 taaatgagga ttctcaggac aacaacattt tttgttgatc actgtgcatc ctttaccggt
     1381 cgatacttaa ctaattcctc cccttagtaa tattctggtt caggaatctg aaaccgattt
     1441 aactcatcaa agagttgctg ctcaatcaga gggttaatgg caactattga ccgactcgtc
     1501 gtaccccagg gcttctcatt gatttgtagc accctaccgc cagcctcttc taaaatacag
     1561 acacctgccg ctacatcata aaaagaaatc ccaaactgaa agtaagcttc aaatcgtcca
     1621 caagcgacat aagctattcc taaggcagct gaaccaatgg cgcgtaatgc ttgagtacgg
     1681 cataataaat gtttatatac tggaataatg atatcacaac ggttttgttc gtgaggaaaa
     1741 ccagtcgaga gaaggcttcg gcgcaagtct ttaacatcag ataattgaat ggggttgccg
     1801 ttaagatagg ctccattccc ccgctccgaa aaaaacgttt caccaaaagc aggtgaatcg
     1861 ataacaccaa taactggaat cccttgatga tagtaggcta atgaaatact atagagcggt
     1921 aaaccatgaa tataattggt tgttccatcg atgggatcga gaacccaggt cgaggaaaaa
     1981 tctttcaagg gataaccact ttcctcaccc caggtgatat cttctgggaa atttttctta
     2041 agggttttta atatcaaatc ctgagtttcg cgatcgatat cggtaacaac atccatgggg
     2101 ctggttttat cttcaatatg gcctatagat tgctgtcgct cttgggcaat catcccagca
     2161 attttgacga tatctaatgc tttgtttagg cgatccttaa acatatttat ttctccattt
     2221 ctatcctgaa aatacatgaa taagtgaaaa gtgataaaaa aacagaaaaa ggcatacctc
     2281 ccttcaatcc cccctcaatg ggggaatgaa ttcaacaatt cccctccttg ttcgaacggt
     2341 gtcgctttgc ggcggggagg gtgtctttca ttcgtcatcc tgaggcttcg tttttcgaag
     2401 ccgtgaggat ctcattccgg attctcaccc tcatcctgac cttctcccat caagggagaa
     2461 ggaacttttg tcatcatttg taaggagtgg agcgatgtgg caatcttata agctcaacct
     2521 tttatttatc ttactaaata atattacatt cttactcccc taaacaagtt ccagtggaac
     2581 gagcaacctg cagcaaggaa tggcctaaag gtatagtttt aagcttatca atggctttct
     2641 taatgggaac cggtataatt tcattgcctc gcaaagctgg aaacaatcca aattgttctt
     2701 gagcaaccaa atccactgcc gctgctccaa aacggctggc gagtacgcga tcatagggag
     2761 tgggagaacc acctctcaat aaatgaccaa gaataactac ccgggtttca atacccgtct
     2821 tttcttcaat ctgctcacca ataacatggc caatgccacc caa
//
LOCUS       DAOSOL010000185         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_35015_length_2858_cov_3.511951, whole genome shotgun sequence.
ACCESSION   DAOSOL010000185 DAOSOL010000000
VERSION     DAOSOL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35015_length_2858_cov_3.511951"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /gene="dapB"
                     /locus_tag="PK171_05035"
     CDS             complement(<1..529)
                     /gene="dapB"
                     /locus_tag="PK171_05035"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOT05583.1"
                     /translation="MKKIRVMITGVAGKMGQELVRAITQQKDMLLVAGCDISQVGKDA
                     GEMCGIDAIGVIIEMDLGVTLREVRPDVVCDFTFGEALRKNLPLYLENRVNMVIGTTG
                     LTPEELKNLEDECKQREIGCLVAPNFAIGAVLMMQFAQKASQYMDRAEIIEFHHPQKK
                     DAPSGTAIKTAEGIIQ"
     gene            complement(532..1860)
                     /gene="lysA"
                     /locus_tag="PK171_05040"
     CDS             complement(532..1860)
                     /gene="lysA"
                     /locus_tag="PK171_05040"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT05584.1"
                     /translation="MLGNKAFNQKNHLEIAGCDIVQLAEEFGTPLYVYDEALLRSNMK
                     AYQNQAKKLYPNSTVAYAGKAFLCSAMCRLLDQESMWLDVVSGGEYYVARESGFPTER
                     LLFHGNNKSPQERELVLKNGIGRWIIDSEWELTLLSKEAKKYLHGKLPVFFRITPGID
                     PHTHAYITTGKVDSKFGLTLVDGIAQRVIRQALSCESLEVKGLHCHIGSQIDSLEPFI
                     NTIKAMIRFMANFKEQNNLVFSELDLGGGLGVAYLEEQKGNFPSVKEYTEAIVETIRE
                     ECKRYQYPPPKLFIEPGRSIVNDAGNTVYTVGTIKEIPGIKKYIAVDGGMTDNPRPIL
                     YGAGYQAIVANRIHQGSPEMVSVVGKCCESGDVIIQQTEISPVQTGDILVVEGTGAYN
                     YSMASNYNLIPRPGVVFIRNGKPYLVVRPESWYDLVRRDILPEYLIEKGE"
     gene            complement(2004..2348)
                     /locus_tag="PK171_05045"
     CDS             complement(2004..2348)
                     /locus_tag="PK171_05045"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HOT05585.1"
                     /translation="MLKKLSFIEKSKIVKSYEIQDFKQGKDFYYIKIKLVFTDESEFY
                     VREFISEKDYLYSYHWQNKDGKLRIRWDNAPHHKDMKTFPHHKHSPKLEESTEIGFEE
                     ILLFIEEKMKKE"
     gene            complement(2323..2550)
                     /locus_tag="PK171_05050"
     CDS             complement(2323..2550)
                     /locus_tag="PK171_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05586.1"
                     /translation="MISQSTPIKEKIKFFEKKYGSSFNIFEREIKEEIKEKEDFQKWD
                     DYIEWKAYFEKLKDLELKLKEIENAQEVKLY"
BASE COUNT          808 a          629 c          447 g          974 t
ORIGIN      
        1 cctgaatgat tccttcagca gtcttaattg cggtcccgga aggtgcatct tttttttggg
       61 gatggtgaaa ctcaataatc tcggctctat ccatatattg agaagctttt tgggcaaatt
      121 gcatcattag cactgcacca atagcaaaat tgggagctac caaacaacca atttctctct
      181 gcttacattc atcttccaaa tttttgagct cttctggagt gagaccggta gttcctataa
      241 ccatattcac tctattctca agatataatg gtaaattttt tctcaaagcc tcaccaaaag
      301 taaaatcaca aacgacatca ggtcttactt cccgcaaggt aactcctaaa tccatttcta
      361 taataactcc gatcgcatca attccacaca tttctccggc atccttgcca acctgggaaa
      421 tatcacagcc agcaaccagc agcatatctt tttgctgggt aatggcgcga actaattctt
      481 gacccatttt tcccgctacc ccagtaatca ttactctaat ttttttcatt ttcattctcc
      541 cttttcgata agatactcgg gaaggatgtc tctacgaact aaatcatacc agctttcagg
      601 acgaactacc aaatatggtt ttccgtttcg aataaacacg actcccggtc gaggaatgag
      661 attataattt gaagccatgg aatagttata agcaccggtt ccttcgacca ccaagatatc
      721 gccggtttgg accggactga tttccgtttg ctgaataata acatcacctg actcacagca
      781 cttcccaacc actgaaacca tttcaggaga tccctgatga atccggttgg ctacgatcgc
      841 ctgataacca gcaccataaa gaattggtcg ggggttatca gtcattccac cgtcaacagc
      901 gatatatttt ttaattccag gaatctcctt gatcgttccg acggtataga cagtatttcc
      961 ggcatcgttc acaatcgaac ggcctggttc aataaataat ttgggaggtg ggtactgata
     1021 tcttttgcac tcttcgcgga tcgtttcaac aatcgcttca gtatactctt tgaccgaggg
     1081 aaaattccct ttctgttctt ccaggtaagc aacacccaaa ccaccaccca agtctaactc
     1141 ggaaaacacc aagttatttt gctctttaaa atttgccata aaccgaatca tggctttaat
     1201 ggtattaatg aaaggttcca aggaatcgat ttgtgaacca atatgacaat ggagtccttt
     1261 tacctcaagg ctttcacagc ttagcgcttg tcgaattacc ctttgggcga ttccatctac
     1321 caaagttaat ccaaatttac tatcgacttt accagtggta atataggcat gagtatgagg
     1381 gtcaatacct ggagtaatcc ggaaaaaaac tggcaacttc ccatgaaggt attttttggc
     1441 ttctttactg agaagagtaa gctcccattc gctgtcaata atccatcgtc ctattccatt
     1501 ttttaaaacc aattcccgtt cttgcgggct cttgttattg ccatgaaata ataatcgttc
     1561 ggtgggaaat ccactttctc gggcaacata atattcgcca cccgaaacaa catccaacca
     1621 catcgactct tgatcaagaa gacggcacat cgccgagcaa agaaaggctt taccggcata
     1681 agcaacggta ctattcgggt atagtttctt cgcctggttt tgataggctt tcatattgga
     1741 acgcaacagt gcttcatcgt atacatagag tggagttcca aactcttcgg ctaattggac
     1801 aatgtcacac ccagctattt ctagatggtt tttttgatta aaggctttgt ttccaagcat
     1861 ggttatccct tccgcggcaa aaaatcattt ttgaagtcat aacatatgaa aaccagtttg
     1921 tcaatggaag gatttgacag ttttatataa gggtgtggct ttatgccaaa ttgaaatttc
     1981 tcaattttag aaaaatggtt tctttattct tttttcattt tttcttcaat aaatagcagg
     2041 atttcttcaa aacctatttc ggtactttct tccagttttg gagaatgctt atgatgaggg
     2101 aaagtcttca tatctttatg atgaggagca ttatcccatc gaattcttaa ctttccatct
     2161 ttattttgcc aatggtatga atagagataa tctttctcag aaataaattc tctaacataa
     2221 aactcgcttt catcggtaaa aacgagtttt atctttatat aataaaaatc tttcccttgc
     2281 ttgaaatctt gtatttcata cgattttaca atcttacttt tttcaataaa gcttaacttc
     2341 ttgagcattc tcaatctctt ttagttttaa ttccaggtct tttaattttt caaaataagc
     2401 cttccattct atgtaatcat cccacttttg gaaatcttcc ttctctttta tttcttcttt
     2461 tatctcgcgc tcaaatatat taaagcttga tccatatttt ttttcaaaaa actttatctt
     2521 ttctttaata ggtgtgctct gagaaatcac ttctaatttc tctaaaattt ttatctcttc
     2581 ctttgatata tttattatga tcgaaactca cctccatatt tttgttctgc cctcatattg
     2641 attaaatgag agttcaggtt tttaaatttc ctttactcat tcaccaattg aacttcattt
     2701 atatagtgga attattttcc aatactaaac aaatctcatt tatcgtcttt tattcttgtc
     2761 ttcaatttat tatacactta tccagttcca cagttgcaat tcactttgca ttatcttaat
     2821 aactcagtac caggcaccgc taagtttcta accttgag
//
LOCUS       DAOSOL010000186         2834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_35393_length_2834_cov_3.429651, whole genome shotgun sequence.
ACCESSION   DAOSOL010000186 DAOSOL010000000
VERSION     DAOSOL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35393_length_2834_cov_3.429651"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..1933)
                     /locus_tag="PK171_05055"
     CDS             complement(407..1933)
                     /locus_tag="PK171_05055"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05587.1"
                     /translation="MNQLNSTTHVPLIIMKGIVKRFPGVIALNQVDFELLPGEVHVLL
                     GENGAGKSTLIKVLSGAFPADSGEIFIFGEKVTIESPEVPLKKGLRFIYQELNLVPQI
                     DIARNIFLGVEPLGIRKLGIINTLTLYKTASQLLERFNIQLDPHRIVSTLSVTQQKMV
                     EIARALVTDARVIVLDEPTDVLESTSRNDLFKVINHLKKEGVGFIYISHRYAEVYELG
                     DRVTILRDGKNVGTFSIRDLTLETMIEKMIGGKIGKQYPSLPTPQEKIILRLENFQKG
                     TAVLGIDFMLRKGEILGITGLMGAGKTELARAIAGVDPPTSGNLYIEDKKVTVRTPEE
                     AIRHGISFLTENRKTEGVILDQSLRNNYGLPNINRLSPFGLVKIKKMNDEIDFYMKEL
                     TIKAPHRFTLAGQLSGGNQQKLVLAKWLGTHAKVILFDEPTRGIDIIGRREVYRIMQE
                     LLAEGTSIIMFTSDYAEAMEMSHRVMVMRRGKIVKEFPRNTASEDDILKAAIGVGEFH
                     "
     gene            complement(2171..>2834)
                     /locus_tag="PK171_05060"
     CDS             complement(2171..>2834)
                     /locus_tag="PK171_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT05588.1"
                     /translation="FSQIDAAYPLSGHDGLYFGVQYVLKTLPWAKIANCPNIATTDGL
                     YKPEGLSESDALELMKHAYGTIIEKAERYSININIEVHGYFTTKPEFLDKMLNFAQSD
                     YFGLNFDTGNSFIAGQDPVVFCQQFIKRIKHVHIKDVSTSLADSLRGKETGIGISHAA
                     IGEGVNAENIITVIKMLRDSGYNGVLSIECEGQGGPLIEKSLHWLRKTLQTLNIPEEK
                     SL"
BASE COUNT          820 a          592 c          553 g          869 t
ORIGIN      
        1 gtgcccaccg ccatgacacc caggactgtt gcctgggaaa aaatggtttg gaaattgctg
       61 attgctcggt aggttggtgc aaaaatgaaa aaaatcacat tgatagcaac caatcctaag
      121 agagcttgtg tagcccgttg aaattgcttc aacagggatg atgaaaccat atttcttatc
      181 ctctcttata cactcttttt aaaaagtatc aattgggatc taattgcatt gaatgatgct
      241 tgcttaaatg tttttcttca ttattatcaa ctgaactttt ttaacaactt aaagttactt
      301 taggtcaact ttcttaattt cttttcaaga ctaaaaaata acttcttgct tataaaccac
      361 ctcctcaaac tctctagccg caatgaggcg agagcaatat tgacttttaa tgaaattctc
      421 ccacgcctat ggcagccttt agaatatcat cttcgctagc ggtattacgt ggaaattctt
      481 ttacaatttt gccccgacgc attaccatga cgcgatggct catttccatg gcttcggcat
      541 aatcggaagt aaacatgatg atagaggttc cctcagccag gagttcttgc ataatccggt
      601 acacctctct gcggccaatg atatcgatac cgcgggtagg ttcatcaaaa aggatgactt
      661 tggcatgagt gccaagccat ttggccaaaa ccaatttttg ctgattcccc ccggagagct
      721 gaccggccag ggtaaaacga tgaggtgctt tgatggttag ctctttcatg taaaaatcta
      781 tctcatcatt cattttttta atcttcacca aaccgaaagg gctcagtcga ttaatattgg
      841 gcagtccgta attgtttctt aaggattgat cgagaataac cccttcggtt ttccgatttt
      901 cggtaagaaa tgagatgcca tgtcgaatag cctcttcagg agttctcacc gtaacttttt
      961 tatcctcaat ataaagattc ccactagtgg gcgggtcaac accggcaatc gcccgagcca
     1021 gctcggtttt gccggctccc attaagccgg ttattcctaa aatttctcct tttcgaagca
     1081 tgaaatcaat acctagaact gctgtccctt tttgaaaatt ttccagtcga agaatgattt
     1141 tttcctgggg agttggaaga gaagggtatt gtttgccaat tttcccgccg atcatttttt
     1201 caatcatggt ttcaagagta aggtctcgaa tagagaaagt accaacattt tttccgtctc
     1261 gcaggatggt gactctatca cctaattcat aaacttcagc gtagcggtgg gagatataga
     1321 taaaaccaac cccctctttt tttaggtggt tgatcacttt aaaaaggtca ttacgagatg
     1381 tgctttctaa aacatcggtt ggttcatcga gaacgataac ccgagcgtcg gtaaccaaag
     1441 ctcgagcaat ctcaaccatc ttttgctggg taacgctgag tgttgataca attcggtgag
     1501 gatctaattg aatgttgaaa cgctctaaaa gttgggaagc agttttgtaa agggttaagg
     1561 tattgattat ccccaattta cggattccta agggctcaac cccaaggaat atgttgcggg
     1621 caatatcaat ttgaggaacc aaattgagtt cttgataaat gaaacgaagt ccttttttaa
     1681 gagggacttc tggagattca atggtcactt tttcaccaaa aatgaatatc tcaccactat
     1741 cagcaggaaa tgctccactc agtactttga taagagttga ctttcctgct ccgttttcac
     1801 caagcaggac atgaacctct ccaggaagga gttcaaaatc gacctggtta agggcaataa
     1861 cccccggaaa acgtttgaca atccccttca ttattatcag aggaacatga gtggttgagt
     1921 tcaactggtt cattatttcc cctgcctcat tggtttgtgg tgttgctttt agattgacta
     1981 aaagcgaagt aatgcaaccg atttcaattt atcatagaaa atattcactg tcaacagata
     2041 aggcattcat taaaataact gtggtgaata agtgactatt ataccaataa attgaaatcg
     2101 gttgcttaac ggttagcttt tatagcaatt tttcccagac aaaaactcaa cggacttttt
     2161 taaatttagt ctaaaggctt ttttcctctg gaatgttcag agtttggaga gttttcctaa
     2221 gccagtgaag agatttctcg attaatggtc caccctggcc ttcacactca atgctcaata
     2281 ccccattata accagaatct cggagcattt taatgacggt tataatgttt tcggcattga
     2341 ctccttcacc gatagcagca tgactaatac caatgccggt ttctttccct cttaaggagt
     2401 cggccaaact tgttgatacg tctttaatat ggacatgttt aattctttta ataaattgtt
     2461 gacaaaaaac caccggatct tgaccggcaa taaaactgtt gccagtatca aaatttaaac
     2521 caaaataatc gctttgagca aagttgagca tcttatccaa gaattcgggt ttcgtcgtga
     2581 aatacccgtg gacttcgata ttaatgttaa tgctgtatcg ttcggctttc tcaataatgg
     2641 ttccgtaagc atgcttcata agttccaagg catcactttc agagagtcct tctggtttat
     2701 acaacccatc ggtggtagca atattgggac aattggcaat cttcgcccag gggagggttt
     2761 ttaaaacata ctgcacacca aagtataaac cgtcatgtcc cgacaaagga taagctgcat
     2821 caatttgaga aaaa
//
LOCUS       DAOSOL010000187         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_35519_length_2825_cov_3.535018, whole genome shotgun sequence.
ACCESSION   DAOSOL010000187 DAOSOL010000000
VERSION     DAOSOL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35519_length_2825_cov_3.535018"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK171_05065"
     CDS             complement(<1..438)
                     /locus_tag="PK171_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOT05589.1"
                     /translation="MENPISFFKNRKIIDITGELENNLWGYYSLPGMEKVVPPVVIST
                     IASVENQGFFASKISVSTISGTYLEAGSHVLTKGNNLDQHSLNQCILPAKVIQLPTQK
                     PKALITVDLLTQFAPELNPGDALLIGTGWDQQWNKPGFVLDSPN"
     gene            complement(1221..1985)
                     /locus_tag="PK171_05070"
     CDS             complement(1221..1985)
                     /locus_tag="PK171_05070"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOT05590.1"
                     /translation="MKRFANKKVFISGGATGIGEVAALLFAQQGAIVTIFDRDIENGE
                     KCLQKVLDGGGRGIFISGNLIYPEEIEKAIDQSLKTYGGIDILINNAGVESPYSVHEM
                     PIEEWDRVMAVNLRGMYLMAKAVIPIMKKSGGGAIVNTASISGLLGWPISAAYCASKG
                     GVIQLTKQMAVDYAQDNIRVNCIAPGTTLTPLIERIFQQESEPEKAKQQISQMHPLGR
                     FAQPEEIAQAILFLASEDASFITGAVLPVDGGYTAK"
     gene            complement(1982..>2825)
                     /locus_tag="PK171_05075"
     CDS             complement(1982..>2825)
                     /locus_tag="PK171_05075"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOT05591.1"
                     /translation="FRIEFMRKAKDRMTNIEGQLVGKKALITGAGVGIGKAIALRFAQ
                     EGAGVAVHVNRSLENGLQVVQEITHNKGKAILVQGDLTKKQEIEAIVRKATSELGNID
                     ILVYNSGVGTQHSPDMVHTITEEDWDTVLSVNLKAFVFLSQLLLPAMMQAGKGSIITI
                     SSIRGLLGNPVLASYCASKGGMVLLTKQMALDYAKYNIRVNCICPGFIDTEMFRFYLG
                     KQSDPEASRRVFAGMAAMNRIGRPEEIAASAVFFASDVSSFVTGVALPVDGGYTANGV
                     REIQ"
BASE COUNT          798 a          628 c          545 g          854 t
ORIGIN      
        1 atttggtgag tcaaggacaa aacctggctt attccactgt tgatcccaac cggtacctat
       61 taacaaggcg tctccaggat tgagttccgg ggcaaactga gttaataaat cgacggtgat
      121 cagagctttt ggtttttgag tggggagttg aatgaccttg gcaggaagaa tgcattgatt
      181 cagggaatgc tgatcaagat tgtttccctt ggtaagtacg tgagacccgg cttccaaata
      241 cgttcccgat atagtgctga ccgatatttt tgaagcaaaa aatccttggt tttcaactga
      301 agcaatagtg cttataacta ccggtggaac aaccttttcc atacctggca aagaataata
      361 accccaaaga ttattttcta attctccggt tatatcaata atttttcggt tcttaaaaaa
      421 tgatattgga ttctccataa ccttttctcc tttctttttt gtaaataaag agagctgtga
      481 ttgtgagtaa taaataataa aatttcatcc tcattttaaa tgattgattt atgagtaaat
      541 attttaattc ttgaccgatt ctacaaaaat gacataaata tttttcttga aacatcggga
      601 acaaatgaaa agagattaaa attttggaag gcacacctcc ctgaatcccc cgtcaacagg
      661 agaatgaatt catcaattcc cctcctcgga ggggtgccgt tttaaggcgg ggagggtgtc
      721 tttacgtctt cctgaggctt cgcattagga agccgtgagg atctcatctt tttaaatttt
      781 ttcacccttt tctaaagtag gaagcttttt tatgggtttc ttactaccaa agttcatcat
      841 ttttttattt ttttttattc gactcacgtc tcactattca cgactcactg ttttttagga
      901 atgagattgc cacgtcgtcc aaccaaaaaa cggttgacct cctcgcaatg acggatttag
      961 ataggtattt tcatcctcat ctggtactgc taagtagcat gaaagtctat cctgaaaacc
     1021 acgggcatga tgaatcaagc ccctacaaga gaatataaaa tggagtggcg caatttattg
     1081 cgcctgatta atgtagcgac ctgccatggc aggtcgaatc ttggttctca ccctcatccc
     1141 aaccttctcc catcaaggga gaaggtgcaa aaaattaagg tgaggaagaa tgcaatggtt
     1201 gttactttta tctttaaaaa ttattttgct gtatacccac catcaactgg tagcactgct
     1261 ccggttataa acgaagcatc ctccgatgcc aagaaaagaa ttgcctgggc gatttcttct
     1321 ggttgagcaa atctccccaa agggtgcatt tgggaaattt gttgtttcgc cttctccggt
     1381 tcggattctt gttggaaaat ccgctcgatt aaaggagtta gcgttgtccc aggagcgatg
     1441 caatttaccc gaatgttatc ctgagcataa tcaaccgcca tctgtttggt gagctgaata
     1501 actcctcctt tggaggcaca ataagcagct gagattggcc agcctaataa gccagagata
     1561 gaagcagtat tgacaatagc tccgccaccg gattttttca taataggaat aacggctttg
     1621 gccattagat acatacctcg cagattaact gccatcaccc ggtcccattc ttctataggc
     1681 atttcatgaa ccgaataagg cgactccact ccggcattat tgattaaaat atcaatgcct
     1741 ccgtaagttt ttaaagattg atcgatagcc ttttcaatct cttctggata aatcaggttt
     1801 ccggaaataa aaattcctct tcctccacca tccaagactt tctgaaggca tttttcgcca
     1861 ttttcgatat ctcggtcgaa aatggttacg atagctccct gttgagcaaa caacaatgca
     1921 gccacttccc caataccggt tgcccctcct gaaatgaaaa cttttttatt ggcaaatctc
     1981 ttcattgtat ttccctcact ccattagctg tatatcctcc atcgacaggg agagcgacgc
     2041 cggttacaaa agaagataca tcggaagcaa aaaagactgc tgaagctgct atctcttctg
     2101 gacgtccaat ccggttcatt gctgccatac cagcaaaaac tctccgagaa gcttctggat
     2161 ctgattgctt tccaaggtaa aatcggaaca tttcagtatc gataaatcct gggcaaatac
     2221 aatttacccg gatgttgtac tttgcataat caagcgccat ttgtttggtc aaaagtacca
     2281 ttccgccttt tgaagcacag taagaagcta gtaccggatt acctaataaa ccacggatag
     2341 aagatatagt tataattgaa cccttgccag cctgcatcat ggcaggaaga agcagctggg
     2401 agagaaaaac aaaagctttg aggttaaccg aaagtacggt gtcccaatct tcttcggtaa
     2461 tggtatgaac catgtcgggt gagtgctggg ttccaacccc actgttataa actaagatat
     2521 cgatattccc cagttctgag gtagcttttc taacaatggc ttcaatttcc tgtttcttag
     2581 ttagatctcc ttggactaat attgcttttc ccttattatg tgtgatttcc tgaacgactt
     2641 gtaagccatt ttccaagctc cggttaacat ggacggcaac tcccgcaccc tcttgagcaa
     2701 aacgtaaggc aatggctttc ccgataccaa ccccagcacc agtaatcagt gcttttttcc
     2761 caactaactg accttcgata ttcgtcattc tatcctttgc ctttctcatg aattctatcc
     2821 tgaaa
//
LOCUS       DAOSOL010000188         2824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_35538_length_2824_cov_3.208017, whole genome shotgun sequence.
ACCESSION   DAOSOL010000188 DAOSOL010000000
VERSION     DAOSOL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35538_length_2824_cov_3.208017"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PK171_05080"
     CDS             complement(<1..576)
                     /locus_tag="PK171_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05592.1"
                     /translation="MKKISFILIVALVLMTFVFPAYSQDKLLGEGMTIYCQMGGAEGA
                     VATIQRTLGAREAAKALGVKLIEQYSQWQPEKMVQQFKEAIAAQPDGIVIMGHPGSAA
                     MWDLVREADSKGIIVTVNNTPLPDIQEEFLAKGFGYSGVDLFAGGYLTGQAMVKAGLK
                     PGDKAIVYGLFSQPFRGQSEEGMARALEDAGI"
     gene            complement(709..1737)
                     /locus_tag="PK171_05085"
     CDS             complement(709..1737)
                     /locus_tag="PK171_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05593.1"
                     /translation="MIKTEKKGLLAIEGFPVMLVFVVIILAFMKTAPNAFLRPTIYMS
                     FLTYNAPTLVLAAGLTLIITAGEIDLSFPSVIAFCGMVFAYLFHITGMIFIPFLAALA
                     AGAFIGYMNGLILTKVGIPSIMTTLGTNFFWTGLTILISGGVSLNVNPIKGSLLHTLL
                     VGRIGGLFPSQAIWSIGLTIFVWYLLNRHTFGESIMFIGDNVNVARVMGVNVDSTKIK
                     VFTLMGLMGGFAACLLTFEMTNFWTNQGSGFLLPVMAAVFIGGTSITGGEGRVIGTLF
                     GMFIVGSLETGVTASGVGGFWTRLTTGLIMLGSIALNIVLDRTNGKRLCFFDNLKNSV
                     FTKGLKKE"
     gene            complement(1743..2522)
                     /locus_tag="PK171_05090"
     CDS             complement(1743..2522)
                     /locus_tag="PK171_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05594.1"
                     /translation="MGDKNFQYILEMKNVGMRFGNIEALRNINFSVGKNEVVGLLGDN
                     GAGKSTMVKIMTGVLHPTCGELHIRGKKVTSRDYNVKKAHSYGIETVYQDKSLGEKQT
                     LWRNFFIGRQITNGFGFINIKKEKEIANNIIMDMIGFRGVGIGVDSKVNKLSGGERQG
                     IAIGRAMHFDADLIILDEPTVSLSVKEVHKVLQFVEKIRTSGRACVYISHNIGHVYDI
                     ADRFVIIDRGMVVANFSKDEMSLEKLDEFLLRFASKKRGDE"
BASE COUNT          922 a          634 c          426 g          842 t
ORIGIN      
        1 gattccagca tcttcaagtg ctcttgccat tccttcttcg ctttgcccac ggaatggttg
       61 tgagaaaaga ccatatacga tggctttatc tcctggtttt aagccagctt ttaccatcgc
      121 ttgaccagtt aaatatccac ccgcaaataa atcaacacca gaatatccaa acccttttgc
      181 taaaaactct tcctggatat caggaagtgg ggtattattg actgtaacaa taattccttt
      241 actgtcagct tcccttacca aatcccacat cgccgctgaa cccggatgcc ccataattac
      301 aatcccatca ggttgagcag caatggcttc cttaaattgc tgaaccatct tttcaggttg
      361 ccactgagaa tactgttcga ttaatttgac acctaaagct ttagcagctt cgcgggctcc
      421 taaagttctc tgtatagtgg caacagcacc ttcagctcct cccatttgac aataaattgt
      481 cattccctct cctaaaagtt tatcctgaga ataagcggga aaaacaaaag tcatcaacac
      541 taaagcaaca attaatataa aacttatttt tttcattcct tcttcctcct cgtaaaaatt
      601 gaactgataa cttaaaaata gtcttgaata ttcctaattc aatttgaaag cttttccctc
      661 ctttctatta aataattcaa taacttattt tcaattcctt caataaattt attctttctt
      721 taacccttta gtaaaaactg aattctttaa attatcaaaa aaacaaagac gctttccgtt
      781 ggtccgatct aacacaatat tgagagcaat agaaccaagc ataatgagtc cggtcgtcaa
      841 acgagtccag aatccaccta ctcctgaagc agtgactccg gtttcgagag aacctacaat
      901 aaacatacca aacaaggtgc cgattacccg gccttctcca ccagtaattg atgttcctcc
      961 aatgaaaacg gccgccatga ctgggagcaa gaaaccactt ccttgattcg tccaaaaatt
     1021 agtcatttca aatgtaagta agcaggctgc aaaaccaccc attaaaccca ttaatgtaaa
     1081 aaccttaatc ttggttgaat caacgttgac tcccatgacc cgagcgacat tgacattgtc
     1141 tccaataaac atgatgcttt ccccaaaagt atgacgattc aataaatacc aaacaaaaat
     1201 tgtcaaacca atggaccata tggcttgaga aggaaaaaga cccccaattc taccaaccaa
     1261 caaggtatga agaagagaac cttttatcgg attgacgttg agagaaactc ctcctgaaat
     1321 taaaatggtt aaacccgtcc aaaagaagtt ggttcccaaa gttgtcatta ttgaaggaat
     1381 cccaaccttg gtaaggatca aaccattcat ataaccaata aaggctccgg ctgctaaagc
     1441 tgccaaaaaa ggaataaaaa tcattccagt gatgtgaaaa agataggcaa acaccattcc
     1501 acaaaaagca ataactgatg gaaaagaaag atcaatttca ccagcagtga taatcagagt
     1561 caatccagcc gccaaaacca gagtaggagc attataggtt aaaaaagaca tatatatggt
     1621 tggccttaga aaagcattag gtgcggtctt cataaaagct aaaattataa ccacaaaaac
     1681 cagcataacc gggaaacctt ctattgctaa taaacccttt ttctcagttt taatcataac
     1741 tcctactcgt ctcccctctt tttagatgcg aatcttaata gaaattcatc taatttttct
     1801 aaagacatct catctttgga gaagttagct accaccatcc cccgatcaat aattacaaaa
     1861 cggtcggcga tatcgtaaac atgtccgatg ttgtgggata tataaacaca agctcgacca
     1921 gaggttcgaa ttttctcaac aaattgaaga actttatgga cttctttaac cgacaaagaa
     1981 accgttggct catcaagaat gattaaatcg gcatcaaaat gcattgctcg gccaatggca
     2041 atcccttgcc gttccccacc tgaaagtttg ttaactttgg aatcaacacc aataccaaca
     2101 cctcggaacc caatcatatc cataatgatg ttgttggcaa tttctttttc ctttttaatg
     2161 ttgatgaagc caaatccatt ggttatttgc cgtccaataa aaaaattcct ccataaggtt
     2221 tgtttttctc ctaaagattt atcttgatag acagtttcta taccataact gtgggctttt
     2281 tttacattgt agtctcgaga agtaactttc ttgccacgga tatggagctc tccgcaggta
     2341 ggatggagga ctccggtcat aattttaacc atggttgact ttccggcacc gttgtcacca
     2401 agtaagccta ctacttcgtt ttttccaact gaaaaattta tatttctcaa tgcttcgata
     2461 ttcccgaaac gcatcccgac atttttcatt tcaagaatgt actggaaatt cttgtccccc
     2521 attttcaccc caccttaatc catcttctct tcgcagatag tttaattata attttcatat
     2581 tttagtttgt ccagctaaac aagtatcaaa ttttttttat aaataaaatg gttattttct
     2641 ttcatcatta gatagaatca agtttttgga aattttatta acaattcaaa attgaataat
     2701 ttattctaat caattcggaa ctcatttgaa tgaatgatta ggacatcctt atctttttaa
     2761 tctcttccta ttgaaggata aactctcatg tcgccttggt gacaccagat aaggatgaaa
     2821 acac
//
LOCUS       DAOSOL010000189         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_35717_length_2814_cov_3.088800, whole genome shotgun sequence.
ACCESSION   DAOSOL010000189 DAOSOL010000000
VERSION     DAOSOL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35717_length_2814_cov_3.088800"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="PK171_05095"
     CDS             <1..1376
                     /locus_tag="PK171_05095"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HOT05595.1"
                     /translation="SQDHQNRHFLVFFSPIEGNDDHLQDNLSCSVENLDQPVTNDAYL
                     KKSLDAFQNQITQLTIGNTFEVSIANQKALSVPFTGIYQNNQLAWVLTVLIKENQAYS
                     FLLTSEPQKIENYWNQVSQMISSIRFQEKTSIPIFLAPSTPPEQPKKSLQIPTFSELQ
                     EKLSIPKTSVTPPKGKIVFASEKRGGDFAICVMNPDGNDIAPLTGNHHTLAWQPSWSP
                     DGNKIVFTSFIDFHFEIFLMNADGSGEIMITGDTEEKSYPSWSPDGTRIIFSSKPGVS
                     SSSSFDLFGSTYDPQDSEIYIINADGSNRINISNDSSNDVEPSWSPDGTKIAFSSNRD
                     RNYEIYLMNADGTDPVRLTENFTDDHRPMWSPDGTKIAFSSNRDGNYEIYLMNADGAQ
                     VVNLTKNGFTDSEPSWSPDGNYILFVSNRDRNDEIYRMKADGTEVVRLTKNPKDDWNP
                     CWQPKGN"
     gene            1490..2563
                     /locus_tag="PK171_05100"
     CDS             1490..2563
                     /locus_tag="PK171_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOT05596.1"
                     /translation="MVQLTLPPLVPPPVEQEVKPITEKPNNLIISEKIIFSQEFSSNV
                     FLIRAINKNTWQEEKLFTTLPDQQNKTVTMWEPSWAPDGKKAAYVLWYITGEIYLMSL
                     DDQSTTRLTDNDFDDKNPAWSPDGTQIAFSTNRDDNYEIYLMDKEGNNLVRLTENGFD
                     DGDPCWSPDGTKIAFTSHRDGNNDIFTMNADGSNFRNLTRHSADDIDPAWSPNGTQIA
                     FSTNRDDNYEIYLMDTRGTNQQKLTDNPSLDTEPCWSPDGKQILFTSDRSGTSNLYLL
                     TLGQEYAEPFFWKGYLEQIDNYFQLFQNLPQPDDELSKALFTQQLLILTNLILQIKTY
                     QLEFIEKPKYLITWQADWVRVKE"
BASE COUNT          870 a          656 c          532 g          756 t
ORIGIN      
        1 tgagccagga tcatcaaaat cgtcattttt tagtcttttt ttccccaatc gagggaaatg
       61 atgatcatct ccaagataat ttgagctgtt cggttgaaaa tttagatcaa ccagttacca
      121 acgatgccta tcttaaaaaa tctctggatg ctttccaaaa ccaaatcacc caactaacta
      181 ttgggaatac tttcgaggta tcaattgcta accaaaaagc cctatctgtt ccttttaccg
      241 ggatttatca aaataaccag cttgcctggg tcttaaccgt tctcataaaa gagaatcagg
      301 cctatagttt ccttttaact tctgaacctc aaaaaattga gaactattgg aaccaagtca
      361 gtcagatgat atcttccatt cgttttcaag aaaaaacctc cattcccata tttcttgctc
      421 cttctacccc acccgaacaa ccaaaaaaat ctctccaaat accgactttt tctgaacttc
      481 aagaaaagtt atcaattccc aaaacctcag tcacccctcc aaagggaaaa attgtattcg
      541 ccagcgagaa acggggagga gattttgcca tctgtgtcat gaatcctgat ggaaacgata
      601 ttgctcctct aaccggaaat catcatactt tagcctggca accatcctgg tcaccggatg
      661 gaaataaaat cgtattcaca tcctttatcg actttcattt cgaaattttc ctgatgaatg
      721 ccgatgggag tggagaaatc atgatcaccg gcgacactga ggaaaaaagt tatccgtctt
      781 ggtctccgga tggaacccga atcatttttt cgtccaaacc tggtgtttca tccagctcca
      841 gttttgatct ttttggttca acttatgatc cccaggatag cgaaatctac attatcaatg
      901 ctgatggaag caaccgaatc aacatcagca acgactccag caacgatgtc gaaccgtcct
      961 ggtcaccgga tggaaccaaa attgctttct cttccaaccg ggatagaaat tatgaaatat
     1021 atctgatgaa tgccgatgga accgatccag ttcgattaac tgaaaatttt actgatgacc
     1081 atcgccccat gtggtcaccg gatggaacca aaattgcttt ctcttccaac cgggatggaa
     1141 attatgaaat atacctgatg aatgccgatg gagcacaagt tgttaattta accaaaaatg
     1201 gatttaccga ttccgagccg tcctggtcgc cggatggaaa ctacatcctt tttgtctcca
     1261 atcgggatcg caacgatgaa atctaccgga tgaaagctga tggaactgag gtagtcaggt
     1321 tgactaagaa tcctaaggat gattggaatc cctgctggca gccaaaagga aattagccgt
     1381 gaaaaatttt ctattttttc tacttataat gagtttatct ttaatttcaa taccagttca
     1441 aagtcaaaca ttggatcagc tcaccctgcc tcccctatca actcctccga tggttcaact
     1501 tactttgcct cctcttgtac ctcctccggt tgagcaggaa gtcaaaccta ttaccgaaaa
     1561 accaaacaat ttaattatct cggagaaaat tatattttcc caggaattca gttccaatgt
     1621 tttcctcatc cgagcgatca acaaaaacac ttggcaggaa gaaaagcttt tcactaccct
     1681 tcccgaccaa caaaataaaa ccgtaacaat gtgggaacca tcatgggcac cagacgggaa
     1741 aaaggcagct tatgttttgt ggtatatcac tggggaaatt tatctgatga gtctcgatga
     1801 ccaatccacc actcgcctca ctgataacga ttttgatgat aaaaatccgg cttggtcacc
     1861 cgatggaaca cagattgctt tttctaccaa ccgggatgac aattatgaaa tatacctgat
     1921 ggataaagaa ggcaataact tggtgcgact caccgaaaat ggttttgatg atggagatcc
     1981 ctgctggtca cccgatggaa ccaaaattgc ttttacttcc catcgagatg gcaacaatga
     2041 tattttcacc atgaacgccg atggttctaa ttttcggaat ctcacccgac attctgctga
     2101 tgatatcgac ccagcctggt cgcctaatgg aacacagatc gctttttcta ccaaccggga
     2161 tgacaattat gaaatatatc tgatggatac tcggggaact aaccagcaaa aactaaccga
     2221 taatccaagc ttggataccg aaccctgctg gtcgccggac ggaaaacaga tcctttttac
     2281 ctcggaccga agcggaacta gtaatcttta tcttttaacc ctcgggcagg aatacgccga
     2341 acctttcttc tggaaagggt acctagagca aatagacaac tatttccaac ttttccaaaa
     2401 cttacctcaa ccagatgatg aactttcaaa agctcttttt acccagcaac tcctcatcct
     2461 caccaatctt attttacaaa ttaagaccta tcagctggaa tttatcgaaa aaccaaaata
     2521 ccttattact tggcaagccg attgggtaag agtgaaagag taaaagaaag ttctatgtcg
     2581 agggaaacga ggggaatatt ggcaaaaggc cccgaggggg cgagggggag aaagaaatca
     2641 taaaggatta gatcctcacg cgggaaagca cagctcaggg tggcagcact gtttagccgt
     2701 agtctgctat cctgagcact ttccaggata aaccttcatg ctgctttgca acaccagatg
     2761 agggtgaaaa tttaaattcg acatgccatg gcatgtcgct acattaatta aaga
//
LOCUS       DAOSOL010000190         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_35835_length_2807_cov_4.055233, whole genome shotgun sequence.
ACCESSION   DAOSOL010000190 DAOSOL010000000
VERSION     DAOSOL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35835_length_2807_cov_4.055233"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(280..1677)
                     /gene="gltA"
                     /locus_tag="PK171_05105"
     CDS             complement(280..1677)
                     /gene="gltA"
                     /locus_tag="PK171_05105"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOT05597.1"
                     /translation="MSNNRLNGRPKMPQRTVQERIRDFNEVPLGLEKWAAIEEAKRCL
                     QCKKPSCMKGCPVEIDIPGFIGLLAQGKIDDAILKIKEKNSLPAICGRVCPQEEQCEI
                     LCVLGKKGQPIAVGYLERFLADYEREQGIELPKIPAQNGKKVAIIGSGPAGLTCAGDC
                     AKMGYKVTIFEALHSAGGVLIYGIPEFRLPKAIVAQEVDYVKSLGVELELSVVVGKTL
                     TFDDLRQDGFKAFFIGTGAGLPHFMNLPGENLNGIYSANEFLTRSNLMKAYRFPEYDT
                     PTKVGRRVAVIGGGNVAMDSARTALRLGAEEVCLVYRRTKKEMPARIEEIERAEEEGI
                     NCIILTNPIGFIGNENKWVTGIECIQMELGEPDESGRRRPIPVPGSEFVVPADTVVVA
                     IGQGPNPLLLETLPELKLNKRGYIEADEETGVTSIPGVYAGGDIVTGAATVISAMGAG
                     KKAARAIHDYLDNKS"
     gene            complement(1667..2518)
                     /locus_tag="PK171_05110"
     CDS             complement(1667..2518)
                     /locus_tag="PK171_05110"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOT05598.1"
                     /translation="MVKIIKKEVLTPQIKLMVIDAPRVAQHAKAGQFVIVRINEEGER
                     IPLTIADFDPEKGTVTIIFQEVGKSTIQLGELDEGDYMADFVGPLGKEIEEKNFGHVV
                     CVGGGVGIAPVYPKARALKMLDNRITSIIGARSSDLLFWEDRMQSVSDALYVTTDDGS
                     YGEKGFVTSVLDRVIQKEKIDLVIAVGPLMMMKMVSLLTKKYELPTLVSLNPIMVDGT
                     GMCGCCRVTVGKECKFTCVDGPVFDGHSVDYDELLARQRNYLEEEKRAVESWQMKKAE
                     EGVHHVK"
BASE COUNT          807 a          681 c          449 g          870 t
ORIGIN      
        1 ccagcacgaa tagccgccgc ataacctgcc ggcccacctc caataataac cagatcggtt
       61 tgtttcattc ttaacctcca attatgatct tcatcaatcc tggaaaatca gtaactatta
      121 aggagtttaa agatagtatt aaacaactct gaaattatcg tcacctaaac agtcactatc
      181 agccataact ttttgaagtc atggaattct tcatctatta aagaagttgc cacctgaggc
      241 ttcgaatttt aagccgttaa gactttaaat ttttcttctt caagatttat tatctaaata
      301 atcatgaatg gctcgagctg cttttttccc cgcacccata gcagaaatga ctgtagcagc
      361 accggttaca atgtcacccc cagcatatac tcctggtata ctggttacac cggtttcttc
      421 gtcagcttca atgtatcccc gtttattgag ttttaattcc ggtaaggttt cgagcagtaa
      481 aggatttggt ccttgtccaa tagccacgac caccgtatca gccgggacaa caaattccga
      541 acctggaact ggaattggcc gacgtcgacc actttcatcc ggctccccta attccatttg
      601 aatgcattca atgccggtaa cccatttatt ttcattccca ataaaaccaa tgggattagt
      661 gaggatgata caattaatgc cttcttcttc ggctcgttca atttcttcaa ttcgagctgg
      721 catttctttt ttcgtccttc gataaaccaa acaaacttct tcagcaccca atcgcagtgc
      781 ggttcgcgct gaatccatgg ctacatttcc accacctata accgctaccc ggcgaccaac
      841 tttagtggga gtgtcatatt ctggaaaccg ataagctttc atgagattag agcgggttag
      901 aaactcattc gccgaataaa tcccatttaa attttcacca ggaaggttca tgaaatgagg
      961 caatccagct ccggttccaa tgaaaaaggc tttaaagcca tcctggcgta aatcatcaaa
     1021 ggttaaggtt tttccgacaa ccacacttaa ttcaagctca acacccaagg atttgacata
     1081 atcaacctcc tgagcgacga tcgccttggg aagacgaaat tcaggaatac cataaattaa
     1141 aactcctcca gcagaatgga gtgcttcaaa tatcgttact ttatatccca ttttggcgca
     1201 atctccagca caagtaaggc cagccggacc tgatccaata attgctactt tttttccgtt
     1261 ttgagcaggt atttttggta actcaatacc ttgttctctt tcataatcag ctaaaaatct
     1321 ctctaaataa ccaaccgcta tcggttggcc ttttttaccc agcacacata aaatttcgca
     1381 ttgctcttct tgagggcaga ctctcccaca aatagctgga agactatttt tttccttgat
     1441 tttcaaaata gcgtcatcaa tcttcccttg ggctaaaaga ccgataaatc cagggatatc
     1501 gatttcaaca ggacaaccct tcatacaact tggtttttta cactgcaaac atcttttggc
     1561 ctcttcaatt gctgcccatt tctctaaacc aagcgggact tcattaaaat cacggattct
     1621 ttcctgaacc gtacgctggg gcattttggg acgtccgttt aatcgattat ttgacatggt
     1681 gaactccttc ctcggctttt ttcatctgcc agctctcaac agctcttttt tcttcttcaa
     1741 ggtaatttct ttgacgagct aaaagctcat cataatcaac gctatgacca tcaaaaaccg
     1801 gtccatcaac acaagtaaat ttacattcct tcccaacggt tacccggcaa caaccacaca
     1861 taccggtccc gtccaccata ataggattta aacttaccag ggtcgggagt tcatatttct
     1921 tggttaaaag acttaccatt ttcatcatca ttaaaggacc cacagctata actaaatcaa
     1981 ttttttcttt ttgaatgacc cgatcaagaa ccgaagtcac aaaacctttt tctccataag
     2041 aaccatcatc agtcgtcacg taaagggcat cactaactga ttgcatccga tcctcccaaa
     2101 agagaagatc tgaagaacgg gctccaatta tcgatgttat gcgattatct aacattttta
     2161 acgctcgggc tttgggataa accggggcga ttccaacccc tcctcctaca cagactacat
     2221 ggccaaagtt tttttcttct atctctttcc ccaaaggtcc tacaaaatct gccatataat
     2281 ctccttcatc caactctcct aattgtatag tacttttccc aacttcttgg aaaataatgg
     2341 taaccgttcc tttttctgga tcaaaatcag caatagtaag tggtattctt tcgccttctt
     2401 cattaatacg cacaataaca aattgtccgg ctttagcgtg ttgagctact cttggtgcat
     2461 cgataaccat aagtttgatc tgcggtgtga gtacttcttt tttaataatt tttaccacaa
     2521 ttttatcctc ctcttctgta tataccttgt tcaaagtaag gttatttatt ttctaccgat
     2581 tctggtattc acagcaagaa ttatactata ttcctgaacc ataccaaaat tacatttcca
     2641 gcgaaattaa ggttctgaaa gagggtaaga ttaaattaag aagatggata aatactattt
     2701 aggtatgtcg atgagctact cttgaagctc caaatgaatg aggctttact tttgcagtaa
     2761 aacctaaatt ttctatcatc cctgttacct cttgaataat atttcgt
//
LOCUS       DAOSOL010000191         2792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_36082_length_2792_cov_3.785532, whole genome shotgun sequence.
ACCESSION   DAOSOL010000191 DAOSOL010000000
VERSION     DAOSOL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36082_length_2792_cov_3.785532"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PK171_05115"
     CDS             complement(<1..819)
                     /locus_tag="PK171_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05599.1"
                     /translation="MTSRERLIAAMNHQPVDRIPFDLGSTSITGIHVSCLHSLKVSLG
                     LISPREPVKVINPFLMLGEVDEALRRRLDIDTIDLPTLGNSFGCENVNWKPWKLFDGT
                     PVLVPEKFNTVPDMNGNILQYPKGDKSYRPSACMPNGGFYHDPLIRKKEVIESELTIE
                     NQTEEYTILKEETLRFYQAEAERLFRDTQYGIIFRGVAGSNLGDIAHIPGCSLPDPKG
                     IRDFEEWYISLLTRKEFIKDVFARMTEIAIENLKLFYQAVGNKIQVIVVSAADFG"
     gene            963..1826
                     /locus_tag="PK171_05120"
     CDS             963..1826
                     /locus_tag="PK171_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05600.1"
                     /translation="MRLFAKLSISFFLVLIFSFPGKGINPLYTREIPPETPFAVPPSI
                     SVFQDPETGNIVSQQLGEIKILINKNPTPSPEVIKQQILPPAISPTLKPVERNAEIET
                     PIPSPEKEMVPLKKNTNEEWKYLGSGLTGSFQSEFSIQEKNIRTLQISDLQFILFRSK
                     YLLNQNDSNRMKRIIETVIRFIHDQGDTIPNQIPLNVNGTLWTVTINEDELIWGVDNI
                     QEEILADPLGSRYYVIKRYYYDFQQRIITVAQISDIPHQKDEIETKPWTAIVLNSPFQ
                     TLVEYAQNSIQ"
     gene            2193..>2792
                     /locus_tag="PK171_05125"
     CDS             2193..>2792
                     /locus_tag="PK171_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT05601.1"
                     /translation="MEIKSDSLYFHELPIGFFAIHPDGKLIETNRYFNQMFQLKTKDT
                     NFFQLFVSPEASLAWQSLLESNETLTGFEAQFYTQQRKSKWVEINTRKVKNTFNQIFF
                     YVGTIQDITKRKDSELKKESQSSELLTILNQIPHPLYISDPDGYSLLFVNQALQKKLG
                     EVIGQKCHRVLHGFQSPCIFCTNHTIFEDQNSETLIWDFF"
BASE COUNT          942 a          527 c          456 g          867 t
ORIGIN      
        1 tccaaagtca gcagctgata cgacaataac ctgaatttta tttccaaccg cttgataaaa
       61 taattttagg ttttctatgg caatttcggt catcctggca aaaacgtctt tgataaactc
      121 tttccttgtt aaaagagaaa tgtaccattc ttcaaaatcc cgaatccctt tgggatcggg
      181 aagactacaa ccagggatat gagcaatatc acctaagttg ctcccagcta cccctcgaaa
      241 gataatccca tattgagtat ctcggaaaag acgctcggct tcagcttggt aaaaacgcaa
      301 ggtttcctcc ttcaaaatag tatattcttc agtttgattt tctatggtga gctccgattc
      361 tataacttct ttttttcgaa tcaaaggatc atggtaaaaa cctccgttgg gcatacaagc
      421 tgaaggtcga tagcttttat caccttttgg gtattgaaga atgttgccat tcatatcggg
      481 gacggtatta aatttttccg gaactaaaac cggtgttcca tcaaaaagtt tccagggttt
      541 ccaattaaca ttttcacaac caaaagagtt tcctaaagtt ggtaagtcaa tggtatcaat
      601 atcgagtcgt cttctaagtg cttcatcaac ttcccccagc atgaggaaag gattgatcac
      661 cttgactggt tctcgcggag atatcaaacc taaagatacc ttcaaagaat gaagacaact
      721 gacatgaatt ccagtaatgc tcgtactgcc aagatcgaaa ggtatccgat ccactggctg
      781 atgattcatt gctgctatta aacgttctcg agaagtcatg taattatcac ctcgatagat
      841 tatatcattc aaaatgttga aatagaattt aattgaaaac cattccatct tatctataaa
      901 agactttatt caataaatgt aataaaatat tgtaaaaatc ggcatggatt gaggaaaaag
      961 gcatgagatt atttgcaaaa ctttctattt ctttttttct ggttttgatt ttctcctttc
     1021 ccggtaaggg aatcaacccg ctgtatactc gtgaaattcc ccctgaaaca ccctttgcgg
     1081 tgccaccatc aatttctgtt tttcaagatc cagagacagg aaacatagta tcccaacagt
     1141 tgggagaaat aaaaatactt atcaataaaa atcccacgcc cagtccagaa gttataaaac
     1201 aacagattct tccaccggct atttcaccaa ctctgaagcc agtcgaaagg aatgctgaaa
     1261 tagaaacacc aattccatca ccagaaaaag aaatggtccc tcttaaaaag aacactaacg
     1321 aagaatggaa atatttggga agtggactta ctggaagttt tcaaagcgaa ttttccattc
     1381 aagaaaagaa tattcgcacc ttacaaatat ctgatttgca atttatttta tttcgttcca
     1441 aatatttact gaatcaaaac gattctaacc gtatgaaaag gattattgaa acagtcatcc
     1501 gatttatcca tgatcagggt gataccattc cgaatcaaat tcctcttaac gtaaatggaa
     1561 ctttatggac tgttactatc aatgaggatg agttaatctg gggagttgat aatatccaag
     1621 aagaaatact tgccgatcca ttgggttctc ggtattatgt aattaaaagg tattattatg
     1681 acttccaaca aagaattatt acggttgccc aaatcagtga tatccctcat cagaaagatg
     1741 aaatcgaaac taaaccctgg acagcaattg ttctaaattc accatttcag actttggttg
     1801 aatatgccca aaattctatt caataatttt tctattcgtt atataatttc agagatatta
     1861 gcgggttagt cgataaaaaa tatcaaatga cgagaaaaat taacctctct tcataatcaa
     1921 atttcttaga gtgagggaaa taattttcaa ataactaaac ttttcctctt tattctcaac
     1981 ccgaggagat tcggctgaga ataaaatatt agctcaagat cattcttttt tactcgttgt
     2041 cttgataaaa ttaaaaaaac aggacctagt caatatgccc attaattaaa aaatatttat
     2101 aaaggtcttg actattaaga gataatgggg gattttaaga ttatataact ttaacttctt
     2161 gggagataac cataagggag attcggactt aaatggaaat caagagtgat tccctctatt
     2221 ttcatgaatt acctataggt ttttttgcta ttcatcctga tggaaaatta atagaaacca
     2281 accggtactt caatcaaatg ttccaactaa aaaccaaaga taccaatttt ttccaattat
     2341 ttgtttctcc ggaagcgagt cttgcctggc aatcattatt agaaagcaat gaaaccctca
     2401 ctggatttga agctcaattt tatactcaac aacgaaaatc aaagtgggtt gagataaata
     2461 ctcggaaggt taagaatact ttcaatcaaa tatttttcta tgttgggact attcaagata
     2521 ttaccaaaag aaaagattca gaattgaaaa aagaaagcca gtcgagtgaa ctccttacca
     2581 tccttaatca gattccacat cctctctata tctccgatcc tgatggctat tccctcttat
     2641 ttgtaaatca agctctgcaa aaaaaattgg gagaagttat tgggcaaaaa tgtcatcgtg
     2701 ttcttcatgg ctttcagtct ccttgtatat tctgtaccaa tcacaccata tttgaggatc
     2761 agaattccga aactttaata tgggattttt tt
//
LOCUS       DAOSOL010000192         2769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_36475_length_2769_cov_3.928519, whole genome shotgun sequence.
ACCESSION   DAOSOL010000192 DAOSOL010000000
VERSION     DAOSOL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36475_length_2769_cov_3.928519"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PK171_05130"
     CDS             <1..206
                     /locus_tag="PK171_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05602.1"
                     /translation="WHSSDTTYWPLLHGVRSGALREELSYFATCILENKKPTIIHPEE
                     SLEAVRACLAAEESAKKGQVIVL"
     gene            510..1304
                     /locus_tag="PK171_05135"
     CDS             510..1304
                     /locus_tag="PK171_05135"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT05603.1"
                     /translation="MFQVLAVSTNTYHKFSLEDALKGISQAGFQYLELACVRGWTEHF
                     SLQGFSETDVKKLEEKCDLYKLKISSLSGHSDLSQPEGFENLKKAIDLAVMLGVKVVN
                     TGTVEKESDLSLFYQHIPALGNYALNKGIKVGLEIHGDVLKNGEAAKKIMETINHPAI
                     GINYDTGNCIFYGDSLPEEDIVHCLPWIIHIHLKDKIGGYKVWNFPTLGEGMVNFKKI
                     FSILKEKQVDVPMSVEIEYSGSFEHPVTYVDECVKKSYDYLQKVIV"
     gene            1317..2195
                     /locus_tag="PK171_05140"
     CDS             1317..2195
                     /locus_tag="PK171_05140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOT05604.1"
                     /translation="MRKEDFIFYNLRSGQKGNDINIIFPGWKPHDERVVVFSPHDDDG
                     ILGPGSLIQGIPLFGGDVHIIIFCNGSGGYSVIEQKYIITALRSRETARAYNVLGIKE
                     EKIHRLEYDDYSVFPFIGHKLPGGEEGTFRKIMPLLRKLKITRVLVPNGYREHLDHTA
                     TYMVGAFDTPQIGDPVMVDWGQTEPIRSILQYAVWSDFSPEDAMVEGVDTEIRANRAL
                     KAPIEAEKNILKAMEEYQTQAKIISGLLKAREERIISKNQVAEVFLSFEPRPRCEYKK
                     YIHQIEKIDNSGGQSQ"
     gene            2192..>2769
                     /locus_tag="PK171_05145"
     CDS             2192..>2769
                     /locus_tag="PK171_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT05605.1"
                     /translation="MKNIALLGSGFIAGVHMEGWKRIPGANVVAFFEVVPEKVRSFQE
                     KYTIPHFSSFPRLLEEKEIDIVDICLPTFLHRDYVLQAAQAGKHIFCEKPMALNVEDA
                     VAMKNACQLNGVELMIGHALRFWGEYRKAKELVSQGKIGKILSIDAYRLGVSPTWSVI
                     SWILQQNLSGGAALDLHIHDLDFVNWIGGKPI"
BASE COUNT          870 a          446 c          599 g          854 t
ORIGIN      
        1 gttggcattc atcagatact acttactggc cactcttgca tggagtgcga tcgggagcac
       61 tccgagaaga actatcttat tttgcgacct gtattcttga aaacaagaag ccaacgatca
      121 tccaccccga agaatcgtta gaggcagttc gagcttgctt ggcagcggaa gaatcggcaa
      181 aaaaaggtca agttattgtc ctgtaaagag tatattacaa ctcgagttgt ctatcatctt
      241 agttagatag aaatactaga agaaaataaa atacctgctt tatactctga ctttctttcg
      301 gagttaaatc aggataatgt tttatgaaat ataaaagcct catcttctct ttttatggaa
      361 tagatgaggt tgaggtgtga taaagttcct acactcttta ttaccgaata attgtaattt
      421 ttttcaggat tcgactataa atacattaat tctttgaaaa attgaatgga tattgattaa
      481 aaatatttaa atttgaagga ggttttattg tgtttcaggt tttagccgtt tcaaccaata
      541 cttaccataa attttctctc gaggatgcct taaaaggcat aagccaggct ggttttcagt
      601 atttagaatt ggcttgtgtt cggggttgga ctgaacattt ttcattgcaa ggcttttccg
      661 aaaccgatgt taaaaaatta gaagaaaagt gcgacttata caaattaaag ataagtagcc
      721 tgagcggtca ttctgattta tctcagcccg aaggttttga aaatctaaaa aaagccattg
      781 atttagcagt aatgcttggt gtaaaggttg tcaacactgg gacggtagaa aaagaaagtg
      841 acttgtcgtt attttaccaa catattccgg ctttaggaaa ttatgcactc aataaaggaa
      901 ttaaagtggg gcttgagatc cacggtgatg ttcttaaaaa tggagaagca gctaagaaaa
      961 tcatggaaac aattaatcat ccagccatag gaatcaatta tgatactggg aattgcattt
     1021 tttatggtga tagtttgcca gaggaagata ttgttcattg tcttccctgg attattcata
     1081 tccatctaaa ggataagata ggcggctata aagtatggaa ctttccaacg ttaggcgagg
     1141 gaatggttaa ttttaaaaag attttcagca tccttaagga gaaacaagtt gatgtaccaa
     1201 tgagcgtaga aattgaatac agcggctcat ttgaacatcc agttacttat gttgatgaat
     1261 gcgttaaaaa atcctatgat tatcttcaaa aagttatagt ataggaggca aacgctatgc
     1321 gtaaagaaga cttcattttc tataatctcc ggtcgggtca aaagggtaat gatattaata
     1381 ttattttccc tggttggaaa ccgcatgatg aacgggttgt agtgttttca ccacacgatg
     1441 atgatggaat tcttgggcca ggatcgctta ttcagggcat tcccctgttt ggaggcgacg
     1501 ttcatattat aatattttgc aatggttctg gtggttatag tgttatagaa caaaaatata
     1561 ttattactgc gcttcgatca agggaaacag ctcgagctta taatgttttg gggattaagg
     1621 aagaaaaaat acaccgtctt gaatatgatg attatagcgt tttccccttt atagggcata
     1681 aacttcctgg aggcgaggaa ggtacattta ggaaaattat gcctctgctc cgtaagttaa
     1741 aaattactcg tgttttagtc cccaatggat atcgagaaca ccttgatcat acagctacat
     1801 atatggtcgg agcttttgat actcctcaaa ttggcgatcc agtaatggta gattggggtc
     1861 aaactgaacc cattcgttcg atcctacaat atgctgtttg gagcgatttt tctcctgaag
     1921 atgcaatggt tgagggagtt gacaccgaaa ttcgagccaa tcgtgcattg aaggctccta
     1981 tagaagcgga aaagaatatc ctgaaagcca tggaggaata tcaaacccag gcaaaaatca
     2041 tatcaggact attaaaagca cgagaagaaa gaataatttc aaaaaatcag gttgctgaag
     2101 tttttctttc ttttgaaccg cgacctcgat gtgagtataa aaaatatatt catcaaatag
     2161 aaaagattga taattctgga ggtcaatctc aatgaaaaat attgcattgt tgggttctgg
     2221 ttttattgct ggggttcata tggagggttg gaaaagaatc ccgggggcta atgtagttgc
     2281 cttttttgaa gtagtacccg aaaaagtccg gagttttcaa gaaaaatata caattcctca
     2341 cttttcttca tttcctcgtc tacttgaaga gaaagaaatc gatattgttg atatttgttt
     2401 accaaccttt ttacatcggg attatgtttt acaagctgct caagccggga aacatatctt
     2461 ctgtgaaaag ccgatggctt taaatgtcga agatgccgtg gcgatgaaaa atgcctgtca
     2521 gctgaacggt gtcgaactga tgataggtca tgctctccgg ttttggggag aatataggaa
     2581 agctaaagaa ttagtttctc agggaaaaat cggaaaaata ctctccatcg atgcctatcg
     2641 attaggtgtt tcacccactt ggtcggtaat aagttggatt cttcagcaaa atttaagtgg
     2701 cggggcagcg ttagaccttc atattcatga tttggatttt gttaactgga ttggaggaaa
     2761 gccgattga
//
LOCUS       DAOSOL010000193         2768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_36495_length_2768_cov_3.468485, whole genome shotgun sequence.
ACCESSION   DAOSOL010000193 DAOSOL010000000
VERSION     DAOSOL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36495_length_2768_cov_3.468485"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PK171_05150"
     CDS             <1..219
                     /locus_tag="PK171_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05606.1"
                     /translation="TLSDIHQIKLKAWVGKRAKIMIDQIEGLNASGRTTDNQRIFLPN
                     SNLFLGQVISVVVINTLKGKLYGELVLS"
     gene            197..1168
                     /gene="miaA"
                     /locus_tag="PK171_05155"
     CDS             197..1168
                     /gene="miaA"
                     /locus_tag="PK171_05155"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT05607.1"
                     /translation="MENWFFPDNLDPMPILCLAGPTASGKTDLSIFLAQKLGKVELIY
                     ADSMAIYKYLNIGTAKPSIEQRGLIPHHLIDLLEPDQIWSSFRFRVEAFRCILECLER
                     HSLPIIIGGTGFYLKSLYQPSIPQGSPAHPRLRLLLERYSNPQLFAYLVAIDPPRAFQ
                     IGKNDRKRLIRALEIYHQTGKPPTSFKKITSKTRNPFQFILIGLDHKRVDLKRRIIER
                     TQLMIKQGLIQETQDILLKGFSPNVPALKNFTYGPVVQCLQGRLTIQQMEKRITIGTF
                     QYLKRQLTWFRKAPVLWISTEGKNFDIISDEIIRIFLTYCIGGHSNE"
     gene            1161..1469
                     /locus_tag="PK171_05160"
     CDS             1161..1469
                     /locus_tag="PK171_05160"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOT05608.1"
                     /translation="MNEKNQEKIPPQKITDLGYYFLNVIQAKAWQYLGLLVHPENGET
                     LIDLKEARRAIDLFELIFENLKNDFPSSIKKEMEMHLTNLQLNYVEKVKKEEEQSEKK
                     "
     gene            complement(1536..>2768)
                     /gene="hflX"
                     /locus_tag="PK171_05165"
     CDS             complement(1536..>2768)
                     /gene="hflX"
                     /locus_tag="PK171_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT05609.1"
                     /translation="SGHDNQTNMGADGVCQELLEISRSAGFQMSEIRDVKVLHPFPHY
                     YLGKGKVEEIKVFLQKNPDIRGVIIDTEISPSQQKNLEKAFNIKVYTKIALIHRIFAA
                     RARSSEGKIKVAVASLQYELSRLSGRGVEMSRLGGGIGTRGPGEQKIETERRRIKQKI
                     AQLKRELVKVNRRREVQKSLRIKKNLPIVAIVGYTNAGKSTLLNSLTNAHAYVEDRLF
                     ATLDPTARKAFLPGIGSVVFTDTVGFIREMPETLIDAFRATLDEINDADCLLEVLDAS
                     DPSYPEHHKVINRILQNMAISCRPKIIVFNKIDMVEPVPVISRDWFDDYPVVMVSAEK
                     KIGIGNLIEMIGKLLRNNRESLNITVSYQNLPQIEKEIYQHGYIENIEYRIDGMVEVH
                     CSISREATGRIRQLDFSS"
BASE COUNT          854 a          569 c          487 g          858 t
ORIGIN      
        1 actctttctg atattcatca aataaagtta aaagcctggg tcggcaaaag agcaaagatt
       61 atgattgatc aaatagaagg attgaatgct tcaggaagaa cgaccgataa tcaaagaatc
      121 tttcttccca attcaaattt attccttgga caagtaatct cggtcgtagt gataaatact
      181 ttgaaaggta aactttatgg agaattggtt ctttcctgat aatttagatc cgatgccaat
      241 cctttgttta gctggtccaa ctgccagcgg gaaaaccgat ctttccatat ttcttgctca
      301 gaaattaggg aaggtcgaat tgatttatgc tgattctatg gctatttata aatacttaaa
      361 tattggtacc gccaagcctt ctatagagca gaggggtctt attcctcatc atctcattga
      421 tctacttgaa ccagatcaaa tctggagttc ctttcgtttt cgggttgaag cttttcgatg
      481 tattcttgaa tgtttagaaa gacattcgct tccaatcatc attggtggta ccggttttta
      541 ccttaaaagt ttatatcaac cttcaattcc ccaaggaagc cccgcccatc cccgattgcg
      601 actccttttg gaacgttatt ccaatcccca gctttttgct tatcttgtag ctattgatcc
      661 tcctcgtgcg ttccagattg gtaaaaacga tcgaaaacgt ttgattcgtg ctctcgaaat
      721 ttaccatcaa actggtaaac ctccgacctc ttttaaaaaa ataacctcta aaaccagaaa
      781 tccctttcaa tttattctaa ttggccttga tcataagaga gtagacctaa aaagaagaat
      841 catcgaaaga actcagctta tgataaaaca aggacttatc caagagactc aagatatttt
      901 attaaaaggt ttttcaccca atgttcctgc tttaaaaaat tttacctatg gaccggtggt
      961 tcaatgtctt caaggtcgtt taaccatcca acaaatggaa aaacgaatca ctataggaac
     1021 ttttcaatat ttgaaaagac aattgacttg gtttcggaaa gctccggttc tttggatttc
     1081 taccgaggga aaaaatttcg atataataag tgatgaaatt attcgcatct ttttaaccta
     1141 ttgcatagga ggtcattcaa atgaatgaaa aaaaccagga aaagatacca cctcaaaaaa
     1201 ttactgactt agggtattac tttttaaatg taatacaagc aaaagcctgg caatatttag
     1261 gattgttggt tcatcctgaa aacggtgaaa ctctaattga tttaaaagaa gctcgtcgtg
     1321 ccatcgatct ctttgaatta atttttgaga acttaaaaaa tgatttccct tcatcaatta
     1381 aaaaagaaat ggaaatgcat ctgaccaatt tacaactcaa ttacgtagaa aaagtgaaaa
     1441 aagaagagga gcaaagcgaa aagaaataaa ttttacataa tataccttat gggaaccact
     1501 tctgaaatca aatcaactac ttttcaacca gttttttaag atgaaaagtc caattgacgg
     1561 atgcgtccgg tagcttctcg agatatcgaa caatggactt caaccatacc atcaattcga
     1621 tattcaatat tttcgatata gccatgctgg tatatctctt tttcgatctg aggaagattt
     1681 tggtagctta ctgtgatgtt taagctttcc cgattgtttc tcagcagttt accaatcatc
     1741 tcgatcaggt ttccaatacc aatttttttc tcggctgata ccatgactac tggataatca
     1801 tcaaaccaat cccgtgatat aaccgggaca ggctcaacca tatcaatttt attgaaaacg
     1861 ataatcttcg gtcggcatga tatggccata ttttgcaaga tgcggttaat caccttatga
     1921 tgttccgggt aactgggatc tgaagcgtct aaaacttcaa gaagacaatc ggcatcgttt
     1981 atctcatcca gagtcgctcg aaaggcatca ataagtgttt caggcatttc ccggataaat
     2041 cctaccgtat cggtaaaaac aaccgaacca atccctggca aaaatgcctt tcgagctgtc
     2101 ggatcaaggg tggcaaataa tcggtcctca acataggcat gggcattggt aagactgttt
     2161 aaaagcgtcg acttcccagc attggtatat ccaacaatgg caacaatcgg taagttcttt
     2221 tttatccgaa ggctcttctg aacctcacgg cgccggttca ctttaacgag ttctctttta
     2281 agctgtgcaa ttttttgttt aatgcgtctt cgttcggttt caatcttttg ttctcctggt
     2341 ccacgggtac caatcccacc gcccaagcgg gacatttcga ctccccttcc ggacaatcga
     2401 cttaattcat actgaagtga tgccactgca actttaatct tcccttctga agaacgagca
     2461 cgcgcagcaa aaattcggtg aataagagct atcttagtat aaactttgat attaaaggct
     2521 ttttcgaggt ttttttgttg tgagggtgat atttcggtat caataattac accgcgaata
     2581 tcaggatttt tctgtaaaaa aactttgatt tcttcaacct ttccctttcc cagatagtaa
     2641 tgaggaaaag gatgaagaac ttttacatcc ctaatctcgc tcatttgaaa gcctgcacta
     2701 cgagaaatct ccagtaattc ttgacaaaca ccatccgctc ccatattcgt ctggttgtca
     2761 tgaccact
//
LOCUS       DAOSOL010000194         2763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_36591_length_2763_cov_3.021787, whole genome shotgun sequence.
ACCESSION   DAOSOL010000194 DAOSOL010000000
VERSION     DAOSOL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36591_length_2763_cov_3.021787"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PK171_05170"
     CDS             <1..399
                     /locus_tag="PK171_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05610.1"
                     /translation="ALLSKPDMRSVILHALKNVRTSPREEYKLDFTRLHQLHFDYPVP
                     DRFQGFYLAVKVGCLGGGYPAFFCGADEACVQAFLNHRIRFNHIPILLEKTLETTITK
                     PKSVSDVIDIYNQGYKTAKNLIQQRRSLKC"
     gene            393..1442
                     /gene="rseP"
                     /locus_tag="PK171_05175"
     CDS             393..1442
                     /gene="rseP"
                     /locus_tag="PK171_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT05611.1"
                     /translation="MLTILATIFVIGLLVLCHEFGHFIFARWFKVRVLKFAIGYGPKI
                     WSTQKGDTEYSIRAFPLGGFTKMAGMEDTLIEGYSEVSVPDAERFDKKPIYQRSLIVA
                     GGPAMNLFLSIILVFLVFSFMGVPTSSLKIQQVIENSPAQSAGILPGDVITSINGKTI
                     EKMEDLSNQIALNGEKELSLTVLREQIPIEVQLTPGWDEDEKRFLIGIVYGVENRRVN
                     PLVAFYKSVSSVIEWFVVSFLGLLYMVMGRVPMEVTGIIGIARMSGQAASYGFLNLLY
                     FSALISVALALFNLLPIPALDGGHLLLFLYEKIRGKPMDPSKIGFFYMIGFLFIILMA
                     IFVTYQDVLRIVTGK"
     gene            1439..2512
                     /gene="ispG"
                     /locus_tag="PK171_05180"
                     /gene_synonym="gcpE"
     CDS             1439..2512
                     /gene="ispG"
                     /locus_tag="PK171_05180"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOT05612.1"
                     /translation="MKREKTIQVFLGSVAIGGHSSISVESMGRIYPGRVDECLSEICA
                     AAAAGSDCFRIAVPDERALSGIIQLKKYSPLPLIADIHFSPTIALKAVEAGIDGIRVN
                     PGTWKNQLQYHQLIEKLKAGSGVLRLGANAGSLPSHLQKKGRVEALLDSIIEMLEVPQ
                     KKDFHRIILSAKSTDINETIEVYERLAATFSYPLHVGLTEAGEGFEGIIKSTIALGTI
                     LQKGIGNNIRVSLTSPNPILESQVAVEILRGVGLRQKGIEVISCPTCARTRGDVVSFV
                     REIKAAVQKISSPSPLKLAIMGCEVNGPGEAKEADLGLAFGKSTALLFVRGKVIKTVH
                     QQNALNELIKQLRSMVSNQMVKE"
     gene            2527..>2763
                     /locus_tag="PK171_05185"
     CDS             2527..>2763
                     /locus_tag="PK171_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05613.1"
                     /translation="MKMSALFAPTLKENPAEAEVISHSLMLRAGMIRQLSSGIYNILP
                     LGLRSLEKIMNIVRRELNRADGQELLLPALQPGEL"
BASE COUNT          801 a          510 c          611 g          841 t
ORIGIN      
        1 gcacttctgt ccaaaccgga tatgagatcg gtaattcttc acgctttaaa aaacgtaaga
       61 acttctcccc gggaagaata taaactggat tttacccgac tgcatcaatt acattttgat
      121 tatccagttc ctgatcgttt ccagggtttt tacctcgcag ttaaggtagg gtgcttggga
      181 ggagggtacc cagctttctt ttgtggagcc gatgaagctt gtgttcaagc ttttctaaat
      241 caccgaattc gatttaacca tattccaatt cttttggaaa aaaccttgga aacaacgata
      301 acaaaaccaa aatcggtatc tgatgttatt gatatctata accagggata taaaaccgcg
      361 aagaacctga tacaacaaag gagatcctta aaatgctgac tatcctagct accatatttg
      421 ttattgggtt attagttctt tgccatgaat tcggtcattt tatttttgct cgatggttta
      481 aggtaagagt tctaaaattt gccattgggt atggaccaaa aatctggtca actcaaaaag
      541 gggatactga atattcgata cgagcttttc cgctgggtgg atttaccaaa atggccggga
      601 tggaagatac gctcattgaa gggtatagtg aagtaagcgt tcccgatgct gagcgttttg
      661 ataaaaagcc aatttatcaa agaagcctga ttgtggcagg gggccctgcc atgaacttat
      721 ttttatcgat cattttagtt tttttagtgt ttagttttat gggagttcca accagcagtt
      781 taaaaattca acaagttatt gaaaatagcc cagctcaaag cgccgggata ttacctggag
      841 acgttattac ttcgattaat ggcaaaacta ttgaaaaaat ggaggattta tccaatcaaa
      901 tcgcccttaa cggtgaaaaa gaactatctt tgaccgtatt acgggaacaa ataccaattg
      961 aagtacagtt aactcctggt tgggatgaag atgaaaaacg tttcttgatt ggtattgtct
     1021 atggagttga aaatcgccgg gtgaatccat tggttgcctt ttataaaagt gtttcttcag
     1081 tgatagaatg gtttgtcgtg agctttcttg gcttgcttta tatggtaatg ggacgggttc
     1141 caatggaagt gactggtata attggaattg ctcgtatgtc tgggcaagct gccagttatg
     1201 gatttttaaa tttactttat ttttctgcat tgattagtgt tgctttggct ctctttaact
     1261 tattgcccat tccagcttta gatggaggtc atttattact ttttctttat gaaaaaatcc
     1321 gtgggaagcc tatggatccc agtaaaattg gatttttcta tatgattggt tttttattta
     1381 tcatcttgat ggcaatattc gttacttatc aggatgtttt aagaattgtt actggaaaat
     1441 gaaacgagaa aaaaccatac aagtctttct tggttcagtt gccattggtg gtcattcatc
     1501 aatatcggtg gagtccatgg ggaggattta cccaggacgg gtagacgaat gtctgagtga
     1561 aatttgtgct gcagcagcgg ctggaagtga ctgttttcga attgccgttc ccgatgaaag
     1621 agctttgtcc ggcatcattc agctaaaaaa atattcaccc ctccctctca tagcggatat
     1681 tcatttttca ccgaccattg ccctgaaggc agtcgaagca ggtattgatg gaattcgtgt
     1741 taatcctggt acctggaaaa accagctcca ataccatcag cttattgaaa agttaaaggc
     1801 aggaagtggt gtgcttcggt tgggtgccaa tgccggttca ctcccctctc atcttcaaaa
     1861 gaaggggaga gtggaggcgc tccttgacag cataatcgaa atgcttgagg ttcctcaaaa
     1921 aaaggatttt catcgcatta tcctttctgc caaatcaacc gatataaatg aaactattga
     1981 ggtttatgaa cgcttagctg ccactttttc ctatcctttg catgtagggc ttactgaagc
     2041 tggcgaggga tttgaaggaa tcatcaaatc gacaattgct ttaggtacaa tcctacaaaa
     2101 aggaattggt aataatatac gagtttctct cacatcaccc aatccgattc ttgaatccca
     2161 ggtcgcggta gaaatcctcc gaggagttgg actacggcaa aagggaatcg aagtaatttc
     2221 ctgcccaacc tgtgctcgaa ccagaggaga tgtggtatca tttgtaagag aaataaaagc
     2281 agctgtacaa aagatatcat ctccctctcc cttaaaatta gcaattatgg ggtgtgaggt
     2341 aaatggcccg ggagaagcga aggaggctga tttagggtta gcttttggaa aatcaaccgc
     2401 acttcttttt gttcgaggta aggttattaa aaccgtgcat caacagaatg ctttgaatga
     2461 gctcataaaa caattacgat cgatggtatc caatcaaatg gtgaaagaat aggaggaaaa
     2521 tacgccttga aaatgtcagc tttgtttgct ccaacactga aagagaatcc tgccgaagcc
     2581 gaggtgatca gccatagctt aatgttgcgg gcaggaatga tcagacaact ttcatcggga
     2641 atttacaata tcctcccttt gggtttacgt tctttagaaa aaatcatgaa tattgttcgt
     2701 cgagagctta accgagctga tggacaggag cttttattgc cagctctaca acctggagaa
     2761 ctc
//
LOCUS       DAOSOL010000195         2736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_37086_length_2736_cov_3.431182, whole genome shotgun sequence.
ACCESSION   DAOSOL010000195 DAOSOL010000000
VERSION     DAOSOL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37086_length_2736_cov_3.431182"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1092)
                     /locus_tag="PK171_05190"
     CDS             complement(265..1092)
                     /locus_tag="PK171_05190"
                     /inference="COORDINATES: protein motif:HMM:NF006132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT05614.1"
                     /translation="MESMVEKMFSVKDKVFFFTGGAGILASTLSYELGKLGARIILTD
                     IAPLDSVLDKFRKENIQVTGYPMDALNKQQIQEVSQKVIQEFGKIDALFNAAGGNRKD
                     ATVSPDCSFFDLPLEALKNVVNLNLFGGSIIPSQVFAQLMIENPHGGNIINFSSMTSF
                     RPLTRTIGYSAAKAAVSNFTQWLAVHIAQNYTPKVRVNALAPGFFVTNQNYYLLYKED
                     GTLAPRGEAILSHTPCGRFGIPEDLLSTVLWLLSPASSFVTGIVVPVDGGFSAFSGV"
     gene            complement(1257..1805)
                     /locus_tag="PK171_05195"
     CDS             complement(1257..1805)
                     /locus_tag="PK171_05195"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOT05615.1"
                     /translation="MFMEVVYSQGNIQVVIGDITEETTDAIVNAANSTLLGGGGVDGA
                     IHRKAGSQLKEECRNLGGCPTGEAKITKGYQLKAKYVIHTVGPVWKGGNVQEDELLAS
                     CYKNSLQIAQNYGIQSISFPAISTGVYGFPKKRAAEIAVKEVKKFLKEHPLLVRFVCF
                     DQETAQVYLEVLKNSNSPTPPF"
BASE COUNT          838 a          508 c          550 g          840 t
ORIGIN      
        1 taggcagatt taagtagtat ttcggtcgat aatttggagt agagaagtga atagaatgcc
       61 attatcaaac atcttattat cgatgtgcat aaataaccga aataatcgtt attatttcca
      121 cgaccttggc gttttgaaaa gaagctaagc caaggatgtc ccgttgaaaa ttaagtagtt
      181 ttaaaaaggt accgaccagc atctggtgaa atcgttattc aaggttgagt tggctatagt
      241 ttttttagag ttttcaaagt gactttatac tccagaaaag gcactgaatc ctccatcaac
      301 tggaacgaca ataccggtaa caaagctgga agcgggcgat aaaagccata aaacagtgct
      361 taagagatct tctggaatac caaaccgtcc gcagggtgta tgggaaagga tagcttctcc
      421 tcgaggagcc agagtaccgt cttctttata taaaaggtag taattttgat tggtaacaaa
      481 aaagccaggt gccaaggcat ttacccttac tttcggtgta tagttttgag cgatatgtac
      541 cgccagccat tgggtaaagt tgctcactgc tgctttcgct gccgaatagc caatagtgcg
      601 agttaaaggt cgaaaagaag tcatagaaga gaaattgata atatttccac catggggatt
      661 ttcaatcatc aattgggcga aaacttggga cgggataata gatcccccga agaggttcag
      721 attgaccaca tttttcagag cttccagggg aagatcaaaa aaggaacagt ctggagaaac
      781 cgtggcatct ttcctattac caccagcagc attgaaaaga gcatcgattt taccaaattc
      841 ttgaatcact ttttgggata cttcttgaat ctgctgttta ttcaaagcgt ccattggata
      901 accggtaact tggatgtttt cctttcgaaa tttatcaaga acactatcaa ggggggcaat
      961 atcggtaagt attatcctgg cgcctagctt gcctaattca taggataatg ttgaagccaa
     1021 aattccagct cctcctgtga aaaaaaagac tttatctttt accgaaaaca ttttttcaac
     1081 cattgattcc attttattca tcctccgtat tcattcttac tttttcccat caagggaaaa
     1141 gaaattattg taatatgtta tccttcggtt tagcatttaa atgccataga attttattta
     1201 ttaatgttta ataaaaaatt ttctaaatga gattctcacg tcgcacaata tgctcctcag
     1261 aatggcggag tgggtgagtt gctgttcttt aatacttcaa gatagacctg ggcagtttct
     1321 tggtcaaaac aaacaaaacg aaccaaaagc gggtgttcct tgaggaactt tttaacttct
     1381 tttacagcaa tttcagctgc tcgctttttg ggaaaaccat agacaccagt tgaaatagct
     1441 ggaaaagaaa tactttgaat accataattt tgggcaattt ggagactatt tttatagcac
     1501 gaagcgagga gttcgtcttc ctgaacattt cctcctttcc aaaccggtcc gacagtgtga
     1561 ataacatact ttgctttcag ttgatatcct ttggtgattt tcgcttctcc agttgggcac
     1621 ccccctaagt ttcgacattc ttctttaagt tgagagccag cttttctatg tatagctccg
     1681 tctactccgc ctcctcccag gagggtagaa ttggcagcgt ttacgatcgc gtcggttgtt
     1741 tcctcggtaa tatcaccaat aaccacctga atatttcctt gagaataaac aacttccata
     1801 aacatcaaat acctcacttt cctgaatcat gaaaaagatt tcattattgg acgagaagca
     1861 agatataaaa aaaccaattt tggacttttt ctttcggtag tttatcagaa tttttatcca
     1921 ttatcacgta taaaaggaga agaaaaatct cttgataggg gatgagaata ctttataatg
     1981 taaataattt attggctttc ttatacccaa gggaaagaaa aaaagaaaaa agggagaagg
     2041 tgaggatgag ggtgaaagcc cggaatgaga tcctgattgg ttgctctgta tgagttacct
     2101 ataaaaaatc attctggtat ttacagaata gaccctcatg ctgctttgca gcaccaggtg
     2161 aggatgaaaa ccaagattcg gcatgccacg gcaggttgct acattaatta atgaatgggc
     2221 acaatacatt gtgcccctac gtttttaaat cctttatagg ggcttgattt atcatgccca
     2281 ttccaaatcc gtcattgcga ggaatctaac cgttttttgg ttagacgacg tggcaatctc
     2341 atttagaaaa atgtttttaa agaacaagat aaagattgat attgagagat tctcgcgtcg
     2401 caaaggacgc tccttagaat gacgatgaga aattcccctc caaggaggga aattgttgaa
     2461 ttcattcccc tattgagggg ggattaaggg gggggtgtgc cttgcttttt ctgatctttt
     2521 ttcattcatt ctggtatttt caggatagac atttatacca tttttgcggt accaaatgag
     2581 gataaaagct taggattgga tataccatgg catgatactc tattaaacaa agaccgggcg
     2641 aatttattgc gcccccgcat tttataagtt cttttagagg agcttgattt atcgattcca
     2701 aaattgcaaa attctttatt attaaacacg ctatat
//
LOCUS       DAOSOL010000196         2732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_37151_length_2732_cov_3.650728, whole genome shotgun sequence.
ACCESSION   DAOSOL010000196 DAOSOL010000000
VERSION     DAOSOL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37151_length_2732_cov_3.650728"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            508..1293
                     /locus_tag="PK171_05200"
     CDS             508..1293
                     /locus_tag="PK171_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05616.1"
                     /translation="MSLKFYHFLKILILIVLVVSLVGFAFLMNSCTPEVCTTWSQFCV
                     ATLIGAPSSNEINECPSEVLICPGETAVLYWNVSSDVTSIKITSEFGTTYGPFTATSG
                     SQYVSPLVTTRYTLTAEGKCKSENTVTVHVVAGGETQTLLAKGSPETGVWVVDISPVT
                     TSTSIIVSLIKTLPCTDGSFWNKWGLKKTDLDSTVTFLDVTDVDTSANDIPLVGSWEF
                     TPKDASYQKDTACFLVTIKCTEVYPTPTPSPFPPYSPPPPTHQ"
     gene            1529..>2732
                     /locus_tag="PK171_05205"
     CDS             1529..>2732
                     /locus_tag="PK171_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05617.1"
                     /translation="MSNQLKNILIWGAGKIGRGFIADLFNKAEYNLVFVDSNRELIHQ
                     LNTQQQYTIINLPSLDEKEEVIIKDFQAFHTDEKDQIFQKLKECSILSLVVFPSAFEQ
                     VAKDISAIIERRSREKIDRSLDILMSTNICQPSEQFKHYLFKELSDAGKDYFNRYIGL
                     VDTLIIRMGIEPTPEMREKDPMIILTNGYPELTLDRPAFKGEPPQFKGLLYTTNMAHE
                     EKRKMYTYNTIHAVYAYLGKQRGYQYIIESIQDEEIQQMAVEGLKESSRALQKEFGYS
                     DEEMKEWNNRVLKNMANPILKDKIDRVGADPIRKLKKEDRLIGPALMCIRNGILPYFL
                     AKTAAAALLFTVEDDPATTIIQKFLRSHPIKEAVREFCQLDREVELIQLIAEQYQKFL
                     NKISLKEDF"
BASE COUNT          891 a          529 c          502 g          810 t
ORIGIN      
        1 gaaacaatgg aaaaattcgc gagccagaaa gaaggtatcc tgagatttaa aagaggaacg
       61 aataggtcaa ggggaaagaa aactaaagat ttttttttaa attgcatttt gcttatcaac
      121 taaggatttt agtaaaatta cctcttagct aatggcttaa accaaggttt ttatttatca
      181 accccgattt gacattttag aaggataaag gtaggataat atcaccacgg attctgagga
      241 atcggaaaac cggaggattg gaaaaaagaa cctcccttgc gaaagtgagt gttcgaaacc
      301 tctctaccag gagagaagga agtccagtcc tgaaacactg agggtgttga gtggccatat
      361 aatttccttg gtgagatgaa ggtaggaagg ttcgttagat tccgtggttt ttttattctc
      421 tccttgacct cctttgttaa cccaattcca tattttccgt atttaaatat ataagcagtt
      481 tggttaaatt aaaaggaggg aaaggtcttg agtttaaaat tttatcattt cctaaaaatt
      541 ttaatattaa ttgttttggt cgtttccctt gttggcttcg catttctcat gaatagctgt
      601 actccagaag tatgtacaac ctggtcccaa ttttgcgttg ctactttaat cggcgcacca
      661 tcctcaaacg agattaacga atgtccatca gaagtcctta tctgtcctgg tgaaacagcc
      721 gttctctatt ggaatgtctc aagtgacgta acctcaataa aaattacctc tgaattcgga
      781 actacttatg gaccttttac tgctacatct ggttctcaat acgtaagtcc cctggtcact
      841 acccgttata cccttactgc tgaaggaaaa tgtaaatcgg aaaataccgt tacagttcat
      901 gttgtcgctg gtggagaaac ccaaacctta ttagccaagg gaagcccgga aaccggtgta
      961 tgggttgttg atatatcgcc ggttacaacc agtacaagta taattgttag tcttatcaag
     1021 acgctcccct gcactgatgg tagtttttgg aataaatggg gtttgaaaaa aaccgacctt
     1081 gattctactg tcaccttttt agatgttact gacgttgata cttcagctaa tgatattcct
     1141 ttagttgggt catgggaatt tactcccaaa gatgcctcct atcaaaaaga taccgcttgt
     1201 tttttagtaa cgattaaatg taccgaagtc tatccaactc caacacctag cccttttccg
     1261 ccctatagtc ccccaccacc cacccatcaa taattttttc tggaagtttg aacaagagat
     1321 gatgtatact caaattgagt tgaaaaacat catctcttgt tcataatata aataactttc
     1381 tccttctttt tttataaaat tactgttagg ctgataacta aaaccaatgg atttcaaatt
     1441 tatcttattt cagtcaaaaa cagttctgta aatcttaagt taatcagata tttatattta
     1501 ttgaactcca taagaaagga caaccaacat gtcaaatcaa ctaaaaaata ttcttatctg
     1561 gggagcggga aagattggtc gtgggtttat cgcagatttg tttaataaag cagaatacaa
     1621 cttagtcttt gtcgattcta atcgggaact gatccatcaa ttaaacaccc aacaacaata
     1681 caccatcatc aaccttccca gcttagatga aaaagaagaa gtcatcatta aagattttca
     1741 agcctttcat accgatgaaa aagaccaaat ttttcaaaaa ctgaaagaat gttcgatttt
     1801 atccctggtt gtcttcccat cagcttttga acaggttgcc aaagatatct ctgcaattat
     1861 cgaaagacga tctcgcgaaa aaattgatcg ttctcttgat attctcatga gcaccaatat
     1921 ctgccagcct tcagaacaat ttaaacatta tctttttaaa gagctttccg atgccggaaa
     1981 ggattatttc aatcgataca ttgggttggt agatacctta attatccgaa tgggaattga
     2041 gcccactcca gaaatgagag aaaaagatcc aatgattatt ctcaccaatg gctacccaga
     2101 gcttaccctg gatcgaccag cctttaaggg tgaaccgcct cagttcaaag gcttattata
     2161 taccactaat atggcacatg aagaaaaaag aaaaatgtat acctataata ccattcacgc
     2221 tgtgtatgcc tacctgggga agcaaagggg atatcaatac attattgaat ctatacagga
     2281 tgaggaaatc caacaaatgg cagtcgaagg actcaaggaa tcaagccgag cgctacaaaa
     2341 agagtttggc tattctgatg aggaaatgaa ggaatggaat aaccgagttc taaaaaatat
     2401 ggccaatcct atcttaaaag ataaaattga tcgggtagga gccgatccta ttcgaaagct
     2461 aaaaaaagaa gatcggctta tcggacccgc gttgatgtgc attagaaatg gtatcttgcc
     2521 atatttctta gcaaaaactg ccgcagcggc attattattt accgttgaag acgatccagc
     2581 cacaactatt atccaaaagt ttttgagaag tcacccaata aaagaagccg ttcgagaatt
     2641 ttgtcagctt gaccgggaag ttgaactcat tcagctgatt gctgaacagt atcaaaaatt
     2701 cctcaataaa atatctctta aggaagattt tt
//
LOCUS       DAOSOL010000197         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_37189_length_2730_cov_3.345047, whole genome shotgun sequence.
ACCESSION   DAOSOL010000197 DAOSOL010000000
VERSION     DAOSOL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37189_length_2730_cov_3.345047"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(393..1454)
                     /locus_tag="PK171_05210"
     CDS             complement(393..1454)
                     /locus_tag="PK171_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05618.1"
                     /translation="MKKLWMILITILFVSGIAYAQDLILEKSPEEIIVFAGDSMFYQR
                     ETWRVELQKGENIYSWGKSLTLNDEEVFIQVEGGILRSTISDSSNQNTIVSIYADQPT
                     TGTIHLTFPIEHLHLNYLCQFFWEPDEISPVGRLLVDIMNQSQEAIRNRVFVIANQRL
                     VLNLEPFETKRLIVEEFQMINAEKISRYNPSYFHDTNIHFFWDLLLPDAILFPGKAEY
                     FEKSSSGVVFLGENYLSGETPQLRAEVGESTDVVVEEKVEKQEKLNQIYNQEGKEVLF
                     DTREKKTYRVANRGTEKKKIEVVVPLQAGYELISHSVPLSRTEMNQISFILELDAQQE
                     INVSIEVSGKNLTSGFVFR"
     gene            complement(1451..2638)
                     /locus_tag="PK171_05215"
     CDS             complement(1451..2638)
                     /locus_tag="PK171_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05619.1"
                     /translation="MKRYILVMGGLLLFFILGFSSLSHALLSQSINPDESWLTIYQDI
                     DRAWVAEKVTLSLHQGENRFFLIGDNLLQERFYFHPYNSSTSLKAISTTPTNGYQIEI
                     EAQQAGSYSFLLSFFMSGFNWKERYMAIWDEKNSNFYLYPSVFLSNRKNINWKNVRVS
                     WLLGKPAFLFDGDKGGIPEEAALGAADEVQPQVMMTRERPVEMERISEYQIFHLPYII
                     DLPSQSVLQVFPGLRKFPVQEIIRFDKGNLKRVLKMKSEGDPLPQGWVTIFGENQSVF
                     GTFQFPWVRTQEEFEISVGAIKAFQVERIEKSYKRLKVDWNEDKNIADFWSEVIIEFE
                     LENRGSVPKPVEIIEPLPPEAKLTGSQGWRWEDGNAHLEITIEPEQEETVTLRYEFRE
                     VWS"
BASE COUNT          809 a          591 c          468 g          862 t
ORIGIN      
        1 tccttttctt aggagaatca ggaaaaactg aaaacttcct ccccgccgcg aaagcggcac
       61 ccctccaagg aggggaattg ttgaatccat tctttatctt tttggtttga tcacttttca
      121 cttattccgg tattttcagg atagactttc atgccacatg gcaacaccgg ataaggatga
      181 aaaccaaaat tcaacatgcc atggcatgtc gctacattaa tcgggtgaaa ttaattgcgt
      241 tcctacattt tttcattctt ttgtaggggc ttgatttatc atgcctgcaa ataggttttt
      301 agaatagcaa aaacgttata ctcacctaaa accagccagg aaaggcgttt tcatcatcat
      361 cgaaacctac cgagttgtaa gatggtttcc tgttaccgaa atacaaatcc ggaagttaaa
      421 ttttttcctg atacttcaat gcttacattt atttcttgtt gagcgtctaa ctccagtata
      481 aatgaaattt gattcatctc agttcggctt aaaggaaccg aatgcgagat caactcatat
      541 ccagcctgaa gaggaaccac tacttcaatt ttcttttttt ccgttcctcg gttcgctacc
      601 cgataggttt ttttctctcg agtatcaaag agtacttcct tcccttcctg gttataaatt
      661 tggtttaatt tttcttgttt ttcaactttt tcctcaacaa caacgtcggt actttcaccc
      721 acttcagccc gtaattgggg tgtttcccct gataaataat tctctcctaa aaatactact
      781 ccagatgagg atttttcaaa atattcagct ttgccaggga acaaaatagc atcaggaaga
      841 agaagatccc aaaagaaatg gatattggta tcgtgaaaat agcttgggtt ataacgagaa
      901 attttttcag cgttaatcat ttgaaattcc tcgacaatca gccttttggt ttcaaaaggc
      961 tccaaattga gaaccaatcg ttggttagca atgacaaaaa ccctattccg aatcgcttcc
     1021 tggctttggt tcataatatc aaccaacaac cttcctactg gggaaatttc atcaggttcc
     1081 caaaaaaatt ggcagagata gttcaaatga agatgttcaa tggggaatgt taagtgaatc
     1141 gttccagtgg tcggttgatc ggcataaata gaaacaatag tattttgatt cgatgagtca
     1201 ctgatggtgc ttcgtaaaat ccctccctca acctgaataa atacttcttc atcatttaag
     1261 gtaagcgatt tcccccagga ataaatattt tctccttttt gaagctcaac cctccaggtt
     1321 tctctttgat aaaacattga atctccggca aaaacaatta tttcttcagg gcttttctct
     1381 aatattaagt cctgggcata agctatacca ctcacgaaaa ggatggtgat caatatcatc
     1441 caaagttttt tcatgaccac acctccctaa actcatatct cagggttact gtttcttctt
     1501 gttctggttc gatggttatt tccaggtgag cattaccatc ttcccagcgc caaccttgac
     1561 taccggtgag tttggcctcc ggtggcaatg gctcaataat ctctaccggt ttaggtacac
     1621 taccacggtt ttccaactcg aactcaatta taacttctga ccaaaaatcg gcaatatttt
     1681 tatcttcatt ccagtcaacc tttaatcgtt tataactttt ttcaattctt tctacttgaa
     1741 aagctttgat agcaccaact gaaatttcaa actcttcttg agtccttacc catgggaact
     1801 gaaaggtccc aaaaaccgat tgattttctc caaatatggt cacccatcct tgtggtaaag
     1861 gatctccttc gcttttcatt tttaaaactc ttttgagatt ccctttatca aatcggatga
     1921 tttcttggac gggaaatttt ctcaatcccg gaaaaacctg caggacgctt tgggaaggta
     1981 aatcaataat atagggtaaa tgaaatatct ggtattctga tatcctttcc atctccactg
     2041 gcctttcccg cgtcatcatc acttgaggtt ggacttcatc ggcagctcca agagctgctt
     2101 cctccggaat tccaccctta tctccatcga atagaaaggc tggtttccct aacaaccaac
     2161 tgacgcgaac attcttccaa tttatatttt tccgattaga aagaaagact gatgggtaaa
     2221 gataaaaatt agaatttttt tcatcccaaa ttgccatata gcgctctttc cagttaaatc
     2281 cactcataaa aaaggaaaga agaaaagagt aagatccagc ctgttgagct tcaatttcta
     2341 tctgataacc atttgtagga gtggtactaa tagctttgag tgaagtagat gaattatagg
     2401 gatgaaagta gaatctctct tgaagaagat tgtctccaat taagaagaaa cgattttcac
     2461 cctgatggag tgacagggtg accttctccg ctacccaggc tcgatcaatg tcttgataga
     2521 tggtcaacca agactcatcc ggatttatgg attgggaaag gagtgcatga gatagcgaag
     2581 aaaacccgag aataaagaac aatagaagcc cccccattac caaaatatat cttttcatac
     2641 ttcacctcca ttgaaatcaa tcatgatcaa atctgattaa aactcgttta ctctttcttt
     2701 tatagataac gtatactaaa gttcgcaatt
//
LOCUS       DAOSOL010000198         2729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_37203_length_2729_cov_3.448018, whole genome shotgun sequence.
ACCESSION   DAOSOL010000198 DAOSOL010000000
VERSION     DAOSOL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37203_length_2729_cov_3.448018"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PK171_05220"
     CDS             complement(<1..452)
                     /locus_tag="PK171_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05620.1"
                     /translation="MNQFTSSVPIYDFAILGAGVAGLSAARILSPSLKGVVIDSEEQA
                     GGLAWQLGCKATDRCLYCGVCHGIQLRRDLGNFSSYPFDFILGQQLSSMFKENGNYHL
                     LFKSGRSIITHSILIATGIQPFDAYQIPQYGYGTFPHIYTGIEIERNL"
     gene            complement(452..2146)
                     /locus_tag="PK171_05225"
     CDS             complement(452..2146)
                     /locus_tag="PK171_05225"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOT05621.1"
                     /translation="MMSDLEIAQRSTMKPISEIANTISLNDDDYELYGKYKAKISLDV
                     LNKFQNQPLGKYIDVTAITPTPLGEGKTVTTIGLAMGLNAIGKKSIVCIRQPSLGPVF
                     GIKGGAAGGGYSQVIPMEDFNLHLTGDTHAVSLAHNLLAAFIDNHLHHGNALDLNPYT
                     ISWPRVVDVSDRSLRKIILGLGGKENGVPRESGFDISVASEVMAILALTTDLSDIRKR
                     LGRIVIGYNKKKQPITAEDLKCAGSMAVLMKDAIKPNLLQTLENTPCLVHAGPFANIA
                     HGNSSIIADQIALRLTDYVVTESGFGADCGMEKFMDIKCRYSGLKPNCVVMVCSIRAL
                     KMHSGKYKVVPGKPLDPGLAQEDIEAVTQGSENLIKQIENARYFGIPVVVAINAFTSD
                     SPKEIETVRKISIENGAFDAVVSEVWAKGGDGGKDLAQAVARACDNGGNFQFLYPLDI
                     PIKDKIHAIATKIYGADGVVYENEAEKKIKLFTEMGWDTLPICMAKTHLSLSHDPKLL
                     GRPRGYKLPIRDIRPSIGAGFLYPLCGEMRTMPGLPSKPAGNTVDFDEDGNVVGLF"
     gene            complement(2256..>2729)
                     /locus_tag="PK171_05230"
     CDS             complement(2256..>2729)
                     /locus_tag="PK171_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOT05622.1"
                     /translation="EIYVPEVLIAARAMKSGMEILKPKLAESGVEPVAKMIIGTVKGD
                     LHDIGKNLVAMMMEGAGFEIIDLGIDVPAEKFIQAMKDNKPQVVGMSALLTTTMVQIK
                     ENIKAFNDAGVRSNVKVLIGGAPVTQKFTDEVGADGYAPDAASAVDKVKELLSIS"
BASE COUNT          801 a          589 c          525 g          814 t
ORIGIN      
        1 tttaaattcc tttcaatttc aataccagta tagatatgag gaaaagtgcc atatccgtat
       61 tgaggaattt ggtaggcatc gaaaggttga ataccggttg caattaatat tgaatgggta
      121 attatcgatc gtccgctttt gaagagaaga tgataattcc cattttcttt aaacatcgaa
      181 gataattgtt ggccaagaat aaaatcaaaa gggtacgacg agaaatttcc caaatctcgt
      241 cgtaattgta ttccatgaca tacaccacaa tacaagcacc tatcggtggc tttacatccc
      301 aattgccaag ccaacccacc tgcttgttct tccgaatcga taaccactcc cttcaaggaa
      361 gggcttagaa ttcgagcagc agataaaccg gccacaccgg cacctaggat tgcgaaatca
      421 taaatgggaa cagaagaggt aaattggttc attagaaaag tcccactaca ttcccatctt
      481 catcgaaatc gactgtattt ccagctggtt tggaaggtag accaggcatg gttctcatct
      541 caccacaaag tgggtacagg aaaccagcac caattgaagg ccgaatatca cggattggca
      601 atttatagcc tctcggacgg cctaacagtt tggggtcatg agaaagagaa agatgggttt
      661 tcgccataca aattggtaaa gtatcccatc ccatttcagt gaacaatttg atttttttct
      721 cggcttcgtt ttcgtaaacc actccatcgg caccataaat tttagttgca atggcatgaa
      781 ttttatcttt gattgggata tcaagcggat agagaaattg gaaattccct ccattatcgc
      841 atgcccgagc aactgcttga gccaaatcct ttcctccatc tccgccctta gcccatactt
      901 cggaaaccac tgcatcaaaa gcgccatttt ctatggaaat ttttctgaca gtttcaattt
      961 cttttggaga atcggaagta aaggcgttaa tggctacaac tactggtatg ccaaagtagc
     1021 gggcattttc gatctgttta attaaatttt ctgaaccttg ggtaacagct tcaatatctt
     1081 cttgcgctaa accaggatcg agtggttttc ctggtaccac tttatatttt ccactatgca
     1141 tttttaaagc tcgaattgag cacaccataa cgacgcaatt gggtttgagc ccactatacc
     1201 ggcatttaat atccataaac ttttccatac cacaatcagc accaaaacca ctctcggtta
     1261 caacataatc ggttaaacga agagcgattt gatcggctat gatagaactg ttaccatgag
     1321 cgatattggc gaagggacca gcgtgaacca gacatggagt attttctaag gtttggagaa
     1381 gattgggttt aatagcatct ttcattaaaa ccgccattga accggcacat tttaaatctt
     1441 ctgcagtaat cggctgtttc tttttgttat aaccgataac aatacgcccc aatcgctttc
     1501 gaatatccga taaatcagtc gtcaaagcta aaatggccat gacctcagaa gcaaccgaga
     1561 tatcgaaacc ggattctcga gggactccgt tttcttttcc acccaagcca aggataattt
     1621 ttcgaagcga tcgatcgctt acgtcgacaa cccgtggcca gctgatcgtg tatgggttga
     1681 gatccagggc attcccatgg tgaagatgat tatcaataaa agcggcaaga agattatgag
     1741 ccaaagatac ggcatgggta tctccggtta aatggagatt aaaatcctcc ataggaataa
     1801 cttgggagta tccaccaccg gcagctcccc ctttgattcc aaacactgga ccaagggaag
     1861 gctgacgaat gcagacaatg ctctttttcc cgatggcatt taaacccatg gctaacccaa
     1921 tggtggtaac cgtttttcct tctcctagcg gagttggcgt aatagcagtt acgtcaatat
     1981 attttcctaa gggttgattt tgaaacttat ttaaaacatc gagagagatt ttagctttat
     2041 attttccata gagttcatag tcatcatcgt ttaagcttat tgtgttagca atttctgaaa
     2101 ttggtttcat agttgagcgt tgggcgattt caagatcact catcataact gtaatccccc
     2161 ttttaaaaat aattaataaa gcatttgaat tatttattaa ataagataat taattaattc
     2221 ataattaaaa aaaagggtgg aattccaccc tttttttagg agatacttaa aagttcctta
     2281 actttgtcaa cagctgaagc agcatccggt gcatagccat cagcgccgac ttcatcggta
     2341 aacttttgag taactggagc tcctccaatt aaaactttta cattacttct tactccagca
     2401 tcattgaagg ccttgatgtt ttctttgatt tggaccatgg tggtggttaa taaggctgac
     2461 ataccaacga cttgaggttt gttgtctttc attgcctgga taaatttttc agccgggaca
     2521 tcgataccga gatcgataat ttcaaagcca gctccttcca tcatcatagc aactaaattt
     2581 tttccaatat catgaaggtc tccctttacc gttccgatga tcatttttgc taccggctct
     2641 actccacttt cggccaattt tggttttaat atttccatgc cagatttcat ggcccgggca
     2701 gcaattaaga cttctgggac atagatttc
//
LOCUS       DAOSOL010000199         2714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_37480_length_2714_cov_2.694998, whole genome shotgun sequence.
ACCESSION   DAOSOL010000199 DAOSOL010000000
VERSION     DAOSOL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37480_length_2714_cov_2.694998"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2333
                     /locus_tag="PK171_05235"
     CDS             <1..2333
                     /locus_tag="PK171_05235"
                     /inference="COORDINATES: protein motif:HMM:NF027879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOT05623.1"
                     /translation="FLQEWIEAENDEIKYQLREFSRLLPNSLQPQVDNGIDLFLTDLR
                     RINDEHYQPITRYYWEQIQQLVDQLHNPEMNSQASNEVVSQERYYRNLLERMKGTKGN
                     KFGYLIDYLSSKGVLPSYSFPLHTVELLLPKETKISEHLRLERDLRQAINEYAPGSEI
                     VADKRIWKSLRPIFWKDTPRMLEYRICENCHHLDISDDAGVSLPQTDGVCSVCHEPLR
                     QKSKIRKFVEPDGFIADPKSGKPARQYVNIEPNQMRSALIPEQNLEEEEYGKFICLAY
                     NQKGKLLYVNEGKYGKGFEFPVKAFSIFSENGQKEKLSLGYIQTTNTLHIRFLSYENF
                     VVPSSNDQSFWLSLLYAIIQGACHCLQIERRDIDGVLFPRSSGGSWEQTIVLYDNVPG
                     GAGHVKNIQENLFAVFEEARRILNCNDCAPDTSCTHCLRDYNNQFYYQYLKREFALKF
                     LDLLIASQKPLEINIPGTVRVITSDPANWLFEKIRYARQSVAIAIPKLDLRHPMGENY
                     TWLDTLGDLLNKKCEVSLYLQDLPESTPEGYSLSIQLQVLMNKGLKVWKIAKLPEWQI
                     IIDGSFPEERIICTADKQSSIVLEEQVGTEQLLTSTNHEAVLKIINDWNNLQKIRING
                     DELNPPQHIKVINVRAASKSEINEFFLFESFFAKPCKKMLVNDPYLFDYESIVNRLGK
                     YVSMVFKHSHMVEVVVHTKKASDKRDQEDAEMELNKKFNNSINFIHTAEHDRYIKITR
                     ENGERARIIFGRGLDFIQPDGSTKSTYIVIEDPWLE"
BASE COUNT          907 a          455 c          546 g          806 t
ORIGIN      
        1 actttttaca agagtggatt gaagcggaaa atgatgaaat caagtatcaa ttaagagaat
       61 tttctcgatt actgccaaat agtttacaac cgcaagttga taatgggatt gatctctttt
      121 tgactgactt acgaaggata aacgatgagc attatcaacc aataacgcgt tattactggg
      181 aacaaattca acaacttgtt gatcagcttc ataacccaga aatgaattct caagcaagta
      241 atgaggttgt gagtcaagaa agatattata ggaatttatt ggagaggatg aaaggcacta
      301 aggggaataa gttcggatat ttaatcgatt atctgagtag caaaggagtt ttaccgagtt
      361 attctttccc gcttcacaca gttgaacttt tacttcctaa agaaacaaaa atcagtgagc
      421 accttcgtct ggagcgtgac ttgcgccaag ctataaatga atatgcgcca ggatcagaaa
      481 tcgtagctga taaaagaatt tggaagagtc taagaccaat cttctggaaa gatactccaa
      541 gaatgttgga gtatcgaata tgtgagaatt gtcatcattt ggatatcagt gatgatgctg
      601 gagtgtcatt gcctcagaca gacggagttt gctctgtttg tcatgagcct ctgagacaaa
      661 aatcaaaaat tcgaaaattc gtcgaaccag atggttttat cgcagaccca aagagcggga
      721 agccagccag acaatatgtt aatatcgaac ctaaccaaat gcgttcggca ttgatccctg
      781 agcaaaatct agaagaagag gagtatggta agtttatttg tctggcttat aaccagaaag
      841 gtaaattgtt atatgtaaat gaaggcaagt atggtaaagg ctttgaattt ccggtaaagg
      901 cattttctat cttttctgag aatgggcaaa aagaaaagtt atctcttggt tatattcaaa
      961 ccacgaatac tctccatatt cgatttctaa gctatgaaaa tttcgttgtc ccctcctcga
     1021 atgatcaatc attctggttg tcgttgttat atgctattat tcaaggcgcc tgccactgtt
     1081 tgcagattga acgtagagat attgatggag ttttattccc ccgaagttcg gggggatcat
     1141 gggaacaaac aattgtcctt tatgataatg ttccgggcgg tgcaggtcat gtaaaaaaca
     1201 tccaagaaaa tctttttgcg gtctttgaag aagcccgccg gatcttaaat tgtaacgatt
     1261 gtgcacccga tacgagttgc acacattgtt tacgagacta caacaatcaa ttttattacc
     1321 aatatctgaa gcgtgaattt gcgctaaaat ttcttgattt gttgattgcc agtcaaaaac
     1381 cgcttgaaat caatattcca ggaactgtta gggttataac atcggatccg gcaaactggt
     1441 tattcgaaaa aatacgatat gcaaggcaat cggttgcaat tgctattcca aaattggatt
     1501 tacgtcaccc aatgggagaa aattatactt ggcttgatac gcttggcgat cttcttaata
     1561 aaaaatgtga agtgagttta tatcttcaag atcttccaga atctactccg gaagggtata
     1621 gcctgtcaat tcaactgcaa gtattaatga ataaaggcct taaggtttgg aaaatcgcaa
     1681 agcttcctga atggcaaatt atcatcgatg gcagtttccc agaagaaaga attatctgta
     1741 ccgcggataa acagagctct atcgtactgg aagagcaggt tggcacagag caattattaa
     1801 cgtctacaaa ccatgaagcg gtactaaaaa tcattaatga ttggaacaat cttcaaaaaa
     1861 tacgaataaa tggggacgag ttaaatcccc cacaacatat aaaagtcatc aatgttagag
     1921 ctgcatcaaa gtctgaaatt aatgagttct tcctttttga aagttttttt gctaaaccat
     1981 gtaaaaagat gttggttaat gacccatatc tttttgatta tgaaagtatc gtcaaccgat
     2041 taggaaaata tgtatcaatg gtatttaagc atagtcatat ggttgaggtg gttgttcata
     2101 ccaaaaaagc atcagataag cgagatcagg aagatgctga aatggaatta aataaaaaat
     2161 ttaataattc aatcaatttt attcatacag ctgagcatga tcgttatatc aaaataactc
     2221 gggaaaatgg agaacgagct agaataattt ttggtcgggg actagatttt atccaaccag
     2281 atggctcaac gaaatcaaca tatatcgtaa tagaagatcc atggttagag tgatttgtga
     2341 attgtaaagt aataatggga ttatctgttt ctcccgaaag gattcaagat gtttttctct
     2401 agcgaacgtg atcacgcttc ttgattttag gaaaaacaga aaagctgttt atatcaagaa
     2461 tcaattcgag aagaacttct cgtcaatcct taaagctttg tctatatcat aaatcccgac
     2521 atacggagca atttgagata tcaggcatcg gtagatatac ttttctcgaa tgaaatcaat
     2581 gttcttttta ctcgcatttt tggcgtatgc agagcgattt tatttttcgc caactgcaaa
     2641 actcgggaaa aatctcttat actgctaaaa aaaggcattt tacgattatt tcgtaaaatg
     2701 cctttcatag gagc
//
LOCUS       DAOSOL010000200         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_37488_length_2713_cov_3.929270, whole genome shotgun sequence.
ACCESSION   DAOSOL010000200 DAOSOL010000000
VERSION     DAOSOL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37488_length_2713_cov_3.929270"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PK171_05240"
     CDS             <1..548
                     /locus_tag="PK171_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOT05624.1"
                     /translation="SDNGTPFASLQSPFGLTHLAVWLVKLGIILERIDLGHPEQNSRH
                     ERMHRTLKEEACFPPASNLFTQQDRFEVFKKTYNTVRPHEALGQKTPASWYHKSDRPY
                     PKTLSDCEYPHHTLTRKVDSSGRISLNGNHQVRISKVFTGEFLGFKDYNDSWLVSFSQ
                     YDIGTIDKETHTFESLEYQNA"
     gene            complement(829..1788)
                     /locus_tag="PK171_05245"
     CDS             complement(829..1788)
                     /locus_tag="PK171_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05625.1"
                     /translation="MKNNQRKAFAERNLLIILAVMAVYLIIATKGNFSSWDNITNLIR
                     QSSINGVVAIGMTLIIITGGIDLSVGSIVGLSGMIYALLTSNRGDIQMASSLAIIISL
                     GVSALIGLANGVAVHNGRVPPFIATLGMMTLVRGLVMYVSSGRMISGLPIGFREFSVA
                     TMLGIPALAWTWIFLAIFMAFVLKYTVYGRNLYAIGSNVEAARLSGINIGSNLYKFYV
                     TAALFSGIAGLMLGTRMAAGVPTGGQGYELDAIASVVIGGASLSGGVGTIFGTALGAL
                     IIQTLRNGGNLLGVDPFIMQIIIGAIIILAVFFDQYLKGRKTG"
     gene            complement(1793..>2713)
                     /locus_tag="PK171_05250"
     CDS             complement(1793..>2713)
                     /locus_tag="PK171_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05626.1"
                     /translation="NLEEVFDTCDTVTVLKDGAVVGTYTIEELGSVEKLIPLMVGRTI
                     EEMYYKKEVGIGRELLRVENLSGKKFQNININVKAGEILGIFGLVGAGRTDIARAIYG
                     ADKFDKGKIIVNGKEISIKKPKDSLRSGVGYLPEDRRIQGTFSQQDIEFNINAINLEK
                     VMRAGILIYKKAYEQTKDYVDKLSIKIGSMYQKVSDLSGGNQQKVVIARWLCKNPDVL
                     IFDEPTAGIDVGTKAEIYRLFNEILNQEKGIILISSYLPELIGLSDRILVISNGMVAG
                     EVERKEFSEEYLLTLAMKNIVSKEKIREAV"
BASE COUNT          818 a          660 c          434 g          801 t
ORIGIN      
        1 gcagtgataa cggcactccc tttgcttctc ttcagtctcc ctttggactc actcaccttg
       61 ccgtgtggct ggtcaaactc ggcatcatct tagaacgcat tgaccttggt catccagaac
      121 aaaatagtcg tcatgaacgg atgcaccgaa ctttgaaaga agaagcctgt ttcccaccag
      181 cttcaaatct tttcacccaa caagatcgtt ttgaagtctt caaaaagacc tacaataccg
      241 taagacccca tgaagccctt ggccaaaaaa ccccggcttc ctggtaccac aaaagtgacc
      301 gtccctatcc aaaaacctta agtgattgtg aatatcccca tcatacgctc actcgtaaag
      361 tcgattcctc agggcggatc tctttgaatg gcaaccacca ggtcagaatc agtaaagtct
      421 tcactggtga attcttagga ttcaaagact ataatgattc ctggttagtc agtttctccc
      481 agtatgatat tggtacaatt gataaagaga ctcatacctt tgaatcgctg gagtatcaaa
      541 atgcttaaaa agtgttacct atgtccttag actaaagtgt aacctatgtc tccgttcgta
      601 cagtgccgtt ttacggcggg gagggtgctt ttcatttttt aatggttact ctaaaataat
      661 gaaaaagtta cataaacaaa attttgctaa atgagattct cacgtcgctt tgctcctcag
      721 aatgacggat tgggttgata agattcttgg gtcgatctac aacgtggttg gcgggtttaa
      781 gataagtatt tttcatcatc atttagggcc gtaaagtagc atgaacctct atcccgtctt
      841 tcttcctttt aaatattggt caaagaagac ggcgaggata atgatcgcac caattataat
      901 ctgcataata aatggatcaa cacctaaaag attaccacca ttcctcaaag tttggataat
      961 gagggcaccc agtgcagtac caaatattgt acctaccccc ccactgaggc tcgctccacc
     1021 aataaccact gaagctatcg catcaagttc ataaccctgt ccaccggttg gcactccagc
     1081 cgccatccta gtcccaagca ttaagccagc tataccgcta aaaagagcag ccgttacata
     1141 aaatttatat aaattagaac caatatttat acctgataac ctggcggctt caacgttgct
     1201 ccctatcgca taaagatttc tcccatacac ggtatatttc aaaacaaaag ccataaaaat
     1261 agctaagaaa atccaagtcc acgcaagagc aggaatcccc agcatagtag caaccgaaaa
     1321 ttctctaaaa ccaatcggga ggccggaaat catcctaccg ctcgatacat acatgactaa
     1381 tcctcttacc aaggtcatca ttcccaaagt ggcaataaaa ggtggtactc gaccattatg
     1441 aactgctacc ccattagcta atcctattag cgctgaaact cccagagaaa taataattgc
     1501 cagggatgaa gccatttgaa tatcaccccg gttagaagtt aaaagcgcat aaatcattcc
     1561 ggataagccg acaatggatc caaccgaaag atcaattccc ccagtaataa tgatcagggt
     1621 catcccaata gcaaccactc cattgattga tgactgtcta atcaaattgg taatattatc
     1681 ccaggatgaa aaattaccct tggttgctat gatgagataa actgccataa cagctaatat
     1741 tatcaaaagg ttcctttcgg cgaaggcttt acgttgattg tttttcatcg tcttaaactg
     1801 cctccctgat tttttctttc gaaacgatgt ttttcatcgc taaggttaga agatactcct
     1861 cagaaaactc cttcctttcc acttcaccag caaccatccc attcgaaatc accaggatcc
     1921 gatccgataa tcctatcaat tccggtaaat acgatgatat taaaataata cccttctctt
     1981 gattgagaat ttcattgaac aatctataaa tttccgcctt cgtacccaca tcaatccctg
     2041 cggttggttc atcaaagata agaacatcgg gatttttaca taaccaccta gctataacaa
     2101 ctttttgttg gttccctcca cttaaatccg ataccttttg atacatactc cctattttaa
     2161 tcgagagctt atcaacataa tctttggttt gttcataagc ttttttataa atcaatatcc
     2221 cggctctcat gactttttcc aggtttatag cattaatatt aaattcgata tcctgttgag
     2281 aaaaagtacc ctgaattctt cggtcttctg gaagataacc aactccactc cttaatgagt
     2341 ctttgggttt ttttatcgat atttccttcc cgttgactat aatctttccc ttgtcaaatt
     2401 tatcagcacc atagattgct ctagcaatat ccgttctccc tgctccaacc aaaccaaata
     2461 ttcccaaaat ttctccagct ttaacattga tattaatatt ttggaatttt tttcctgaaa
     2521 gattttcaac ccgaaggagt tcccggccaa ttccaacttc ctttttataa tacatttctt
     2581 cgatggttct accgaccatc aaaggaataa gcttttccac actccccaat tcttctatag
     2641 tataggttcc caccactgct ccatctttga gtacggttac cgtatcacaa gtatcaaaaa
     2701 cttcttcaag gtt
//
LOCUS       DAOSOL010000201         2688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_37953_length_2688_cov_4.281428, whole genome shotgun sequence.
ACCESSION   DAOSOL010000201 DAOSOL010000000
VERSION     DAOSOL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37953_length_2688_cov_4.281428"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..498
                     /locus_tag="PK171_05255"
     CDS             46..498
                     /locus_tag="PK171_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT05627.1"
                     /translation="MFPVGRLDYDSEGLLLFTNDGRITNILTHPRYEVLKTYRVYVSG
                     QVNCRELEVLANGIVKNGRHYQAHSTKLIEHQKGFTIAEVVLNEGKKHEIRILFEGIG
                     HPVLRLIRTKMGPITLDENLLPGEWRYLLKDEIKLLFERGGVSTDVNQ"
     gene            485..1009
                     /locus_tag="PK171_05260"
     CDS             485..1009
                     /locus_tag="PK171_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT05628.1"
                     /translation="MSTNRGFIQVYYGDGKGKTTAALGLAVRATGQGLKVLFLQFFKK
                     NLAGELISLQYLPGITLYQFGSGKFILNRQWQEEDYQEFLMGWEMAKRALHTEDYDLL
                     IMDEFLYAFTYQMLSWDDFLKELNTRKQKIEVIITGRKAIPQLLDYADLVTNMILIKH
                     PYQQGIPARKGIEY"
     gene            1009..1404
                     /gene="aroH"
                     /locus_tag="PK171_05265"
     CDS             1009..1404
                     /gene="aroH"
                     /locus_tag="PK171_05265"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT05629.1"
                     /translation="MEYQKNPMRGIRGAITVDRDSSDEIAQATLLLFETMMKKNHLEP
                     DDITAVFITVTHDLKSAFPARAIRQIEEYRFLPLMCAQEIPIEGTIKRCIRLMMIAFC
                     PNVSHEAIRHVYMREAQNLRRDLKGENNG"
     gene            1397..>2688
                     /gene="aroA"
                     /locus_tag="PK171_05270"
     CDS             1397..>2688
                     /gene="aroA"
                     /locus_tag="PK171_05270"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT05630.1"
                     /translation="MGKVRIIPVSVKLSGDIFCPPDKSFSHRAAMIGALSSGETIIQN
                     FSFCQDTWSTLHCLELLGVKVQALPNQGMVRISSSGKESLSEPSDVLDVGNSGTTLRL
                     ITGIIAGIQGLSILTGDQSIRRRPMKRIIDPLRKTGAVIGGRDRDQYAPLYIIGKKPL
                     QAFHHTLEIPSAQVKSCLLFAALWGDNPSFIKEPILSRNHTENMLQAVGGDIRKQNEV
                     IQVYPGKEFVARPFHLPGDISSAAFLIAVTLLISGSHLIIRNLGLNPTRTGMLDCLQK
                     TGAKINPFDVREEWGERQGHIAIEHSVLNSFEIGQEEIPSLIDEIPILSVLATQAHGR
                     SIIRGAGELRVKESDRLHAISKNLIDLGAQIDETEDGLIISGPVRLKGGKVKSYGDHR
                     IAMSMVIAGLIADAPVEIEDIECISISYPNFFQDLQKIG"
BASE COUNT          806 a          481 c          618 g          783 t
ORIGIN      
        1 gggaagggaa acggttatga aatggattac tgatatcaag gaagaatatt tccggttggt
       61 cgtttggatt atgatagtga gggcttgctt ctttttacca atgatggacg aattactaat
      121 attttaaccc atccccgcta tgaagtgttg aaaacctacc gagtttatgt ttcaggtcag
      181 gtgaattgcc gggaactgga agtacttgcc aatgggattg taaagaacgg aaggcactat
      241 caagctcatt caacaaagct cattgaacat cagaagggat ttacgattgc tgaagtcgtt
      301 ttaaatgaag gaaaaaagca cgaaatacgg attctttttg aaggtattgg tcatccagtt
      361 ttgcgtttaa tacggaccaa aatgggaccg ataaccctgg atgagaattt attgcccgga
      421 gaatggcgtt atctcttgaa ggatgagatt aaacttcttt ttgagagagg tggtgtttcg
      481 accgatgtca accaatagag gattcattca agtatattat ggggatggaa aagggaaaac
      541 cactgcagct cttggcctgg cggtcagggc tactggtcag ggcttaaaag ttctttttct
      601 ccaatttttt aaaaagaacc tagccggtga gctcatttcg ctccaatacc taccgggaat
      661 aactttatat caatttggat cggggaaatt tattttaaat aggcagtggc aggaagagga
      721 ttatcaagaa ttcttgatgg gttgggaaat ggccaaaaga gccttacaca ccgaggatta
      781 tgatttatta ataatggacg aatttcttta cgcttttacc taccaaatgc tcagttggga
      841 tgatttttta aaagaattga atactcgaaa acagaagatc gaggttataa taaccggcag
      901 gaaggcaata ccccagctcc ttgattatgc tgatttggtt accaacatga ttctgattaa
      961 acatccctat caacaaggaa taccggctcg aaaggggatt gaatattaat ggaataccag
     1021 aaaaatccta tgcgagggat taggggagct attacagttg atagagatag ttcggatgaa
     1081 attgctcagg ccaccttgct tttgtttgaa accatgatga aaaaaaacca tttagagccc
     1141 gatgatatta cggcggtttt cataaccgtc actcatgatt taaaatcggc ttttcccgcc
     1201 cgtgcaattc gacaaattga agaatatcgt tttcttccct tgatgtgtgc gcaagagatt
     1261 cccatagagg gtactattaa gcgttgtatt cgtttgatga tgatcgcttt ctgtcccaat
     1321 gtcagtcatg aagccattcg acatgtttat atgcgagagg ctcaaaacct tcggagggat
     1381 ttaaagggag aaaataatgg gtaaggtacg aattattcca gtttcagtca aattgagcgg
     1441 agatatcttt tgccctccag ataaatcatt tagtcaccga gcagcgatga ttggggcctt
     1501 atcttcagga gagactatca ttcagaattt ttccttttgc caagatacct ggtcaacgtt
     1561 acattgtttg gaacttttag gtgtgaaagt gcaagccctt cctaaccagg gcatggttag
     1621 gataagcagt tctggaaaag aaagtttgtc tgagccatcg gatgtccttg atgtgggaaa
     1681 ttcaggaaca accttgcggt tgattaccgg tattatcgct ggaattcaag ggttatcaat
     1741 tttaactgga gatcagagta tacgccggcg ccccatgaaa cgcattattg atccattaag
     1801 aaaaactggc gcggttattg gtggtcgaga ccgagatcaa tatgcacctc tctatatcat
     1861 cggcaaaaaa ccattacaag cttttcatca tactctcgaa attcccagtg ctcaggttaa
     1921 atcttgtttg ctttttgctg cattatgggg agataatcct tcctttataa aagaaccaat
     1981 cctttcgcga aatcatactg aaaatatgct ccaagcggtg ggaggagata ttcgaaaaca
     2041 aaacgaagtg attcaagttt acccgggaaa agaattcgtt gcccgacctt ttcatttacc
     2101 tggagatata tcttcagctg cttttttaat tgctgttacc cttttaattt cagggtctca
     2161 tcttattatt cgaaacttgg gcttgaaccc aacccgaact ggtatgcttg attgtttgca
     2221 aaaaacagga gccaaaatca atccatttga tgtcagggaa gagtgggggg aacgacaggg
     2281 tcatattgcc atagaacact cagttttaaa tagctttgag attggtcagg aagagattcc
     2341 atcgctaatc gacgagattc ccatattatc agtacttgcg acccaagctc atggtcgttc
     2401 gataatcagg ggagctggtg aattgcgagt aaaggaaagc gaccgtttgc atgctatcag
     2461 taaaaattta atcgatttgg gagcacagat tgatgaaacc gaagacggat tgattatttc
     2521 cggtccagtt cgattgaaag gtggcaaggt aaaaagttat ggtgatcacc gaatagccat
     2581 gagtatggtg atagctggtt tgattgctga tgctccagtg gaaatagaag atatagaatg
     2641 tatatcgatt agttacccga atttcttcca agatcttcaa aaaattgg
//
LOCUS       DAOSOL010000202         2675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_38192_length_2675_cov_3.112977, whole genome shotgun sequence.
ACCESSION   DAOSOL010000202 DAOSOL010000000
VERSION     DAOSOL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38192_length_2675_cov_3.112977"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PK171_05275"
     CDS             complement(<1..668)
                     /locus_tag="PK171_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT05631.1"
                     /translation="MSNYIYKVRDTLGATHEGSMEANSERDAVIALRDRGYFITSITE
                     EKPKGLKKEISLFSTSKGGKINLKELALFSRGIATMLEAGVPLIAALESMGEQVSNKG
                     FAIVIEELAGKIERGYSFSQAINEYPKVFNRVFVGMVQSGEAGGNLDWALGRLADYLE
                     WEKDLRDKVQSAMYYPIILIVAMIAASFFMVYFVFPQFLLLFESFSIELPTITKIFLD
                     VIKF"
     gene            complement(668..1738)
                     /locus_tag="PK171_05280"
     CDS             complement(668..1738)
                     /locus_tag="PK171_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOT05632.1"
                     /translation="MDWDVPELLKKVVDSKGSDLHLTVGLPPVIRIDGKLTKTDLPVL
                     KPEDTEYIARFIVGGNERWEQFHKNNELDLSIGLPQTGRFRVNVYRQRGSCGLVLRAL
                     SYTIPSMEELQLPKETLVKLVALPRGLILVTGPTGSGKSTSLASMIDYINNTRACHII
                     TIEDPIEYLHSHKKSIINQRELGADTHSFENALVHALRQDPDVILVGEMRNLETIAIA
                     LTAAETGHLVFATLHTNNAPSTIDRIVDIFPPHQQQQVRIQLAGAIQGVISQQLLSRA
                     NAKGRVPAIEVMLANNAIRNLIREGKSHQIYTIMQTSLSEGMVTMDRSLFELYRKGLV
                     TWEDAFDHAIDQKEFKVWRNRA"
     gene            complement(1911..>2675)
                     /locus_tag="PK171_05285"
     CDS             complement(1911..>2675)
                     /locus_tag="PK171_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOT05633.1"
                     /translation="YRMIERPYGMVLLTGPTGSGKTTTLYSALNHVSSPDLNIITIED
                     PVEYILDDINQIQVKPKIDLTFANSLRSIMRQDPDIIMVGEIRDRETAEIAIHAALTG
                     HLVFSTLHTNTAPGAVTRLQEMGVASFLISSAIIGVVAQRLARKVCEYCKYPVELDGD
                     AALDLTDGKKDHVVVYKGKGCSRCTSGYKGRIAVHSIFDLNDYLREMILNHATERDLT
                     ETARKEGMRTLRENAAIKVLAGIISPEEMYRVTADI"
BASE COUNT          803 a          564 c          510 g          798 t
ORIGIN      
        1 ataaatttaa taacatctaa aaagatctta gtaatggttg gaagttctat gctgaagctt
       61 tcaaaaagaa gaagaaattg ggggaaaaca aaatacacca taaaaaatga agcagctatc
      121 atggctacta ttaaaattat agggtaatac atagccgatt gtactttgtc tcgaaggtcc
      181 ttctcccatt ctaaataatc ggctaatcgt cctaaggccc aatcaaggtt acctccagct
      241 tcacccgatt ggaccatccc aacaaaaact cggttaaaaa ctttgggata ttcattgatg
      301 gcttgagaaa atgaataacc tcgctcaatc tttccggcta attcttcaat aacaatagca
      361 aaacctttat tcgaaacctg ttcacccata ctttcaagag cggcaattaa aggaacgcca
      421 gcttctaaca ttgtagcaat accacgacta aaaagagcta actctttaag atttattttc
      481 cctcccttac tggtagaaaa aagagaaatt tcttttttta ggccttttgg tttctcctcg
      541 gtaatggaag taataaaata gccccgatca cgaagagcaa taactgcatc tctttcgcta
      601 ttggcttcca ttgatccttc atgagttgca cccaaggtat cacggacttt ataaatataa
      661 tttgacatta agctcgattc ctccagactt taaattcttt ctgatcaatg gcatgatcaa
      721 aagcgtcttc ccaggtcacc aagcctttac gataaagctc aaagagcgat cgatccatag
      781 taaccattcc ttctgataag gaggtttgca ttatggtata aatttgatga ctcttccctt
      841 ctcgtatcaa attacgaatg gcattattgg ctaacattac ttcaattgct ggtactctcc
      901 ctttagcatt agcacgggat aagagttgtt gactgataac accttgaata gctcccgcta
      961 attggatacg aacttgttgc tgttgatggg gaggaaagat gtcgactatc cgatcaatgg
     1021 ttgaaggggc attgttggta tggagggtag caaaaaccaa atgcccggtc tcagcggcgg
     1081 tcaaggcaat cgcaatggtt tctaagtttc gcatctcccc caccagaatg acatcagggt
     1141 cttgacgaag agcatgaact aaggcatttt caaaggaatg ggtatcagcg cccaattctc
     1201 tttgattaat aatacttttt ttatggctat ggagatattc aatcggatct tcaatggtta
     1261 taatatgaca cgctcgcgta ttgttaatat agtcaatcat cgacgccaat gaagtggatt
     1321 ttccactacc ggttggtccg gttactaaaa tcaaaccacg aggtagtgct actaatttga
     1381 ccagtgtctc tttgggaagt tggagctctt ccatggaagg gatggtataa ctcaaggctc
     1441 gaagcaccaa accacaggat ccgcgctgtc ggtaaacatt aacgcgaaaa cgaccagttt
     1501 gaggaagacc aatcgaaagg tctaattcat tatttttatg aaattgttcc cagcgctcat
     1561 ttccaccaac aataaatcga gcgatatatt cggtatcttc tggcttcaaa accggcaaat
     1621 cggtttttgt taattttcca tcgatgcgta taaccggtgg taatccaacc gtaaggtgta
     1681 aatcagatcc tttgctgtct acaacttttt taagaagctc tggtacatcc caatccattt
     1741 taatcctacc ttatgtttct cctgaaaact catattcaac catattcctg ctatattgac
     1801 ctctctcttt ggataaaggg agaaggtgct atctattctt tctatagcac aaatatatta
     1861 tcggatactt ttacgaaaag tttagaaaag atgagtgttt tagaggaaaa tcaaatatcg
     1921 gcagtaactc ggtacatttc ttccgggcta attatgcctg caagcacttt tattgcagca
     1981 ttttcacgaa gagttctcat gccttctttt cgagctgttt cagttagatc tctctcagta
     2041 gcatgattta atatcatttc tcgaagataa tcattcaaat caaatatgct gtggacggct
     2101 attctcccct tgtatcccga ggtacaacgg gaacatcctt ttcctttata aaccacaaca
     2161 tgatccttct tgccgtcagt aaggtcgagt gctgcatcac catccaattc tacgggatat
     2221 ttacaatatt cacatacttt cctcgccaat cgctgggcaa caactccaat aatagctgaa
     2281 gaaataagaa aagaagctac gcccatttct tggaggcggg ttaccgctcc aggtgcagta
     2341 ttggtatgaa gagttgaaaa tactaaatgc ccggtgagcg cggcatgaat ggcaatctca
     2401 gcggtctctc ggtctcgaat ttcaccgacc atgataatat ccggatcttg tcgcataata
     2461 gaacggagcg agttagcaaa agtaaggtca atttttggtt taacttggat ctggttgata
     2521 tcatctaaaa tatactcaac tggatcttca atggtaataa tatttaaatc cggactacta
     2581 acgtgattaa gagcggaata aagagtagtg gtctttccgc tgccggttgg tccggtgaga
     2641 agaaccattc cataaggtct ttcaatcatc ctata
//
LOCUS       DAOSOL010000203         2649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_38673_length_2649_cov_4.295682, whole genome shotgun sequence.
ACCESSION   DAOSOL010000203 DAOSOL010000000
VERSION     DAOSOL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38673_length_2649_cov_4.295682"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PK171_05290"
     CDS             <1..433
                     /locus_tag="PK171_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT05634.1"
                     /translation="LNVSAFFIGALALGLRSAAYQTEIFRGAILSLGEGQMLAARSLG
                     MSENQAIFNIILPQALRIALPGWSNEYPILLTDSSVCYAIGVMEIMTRGNQMVTRTYQ
                     PMPIYFACALIFILMNYGGLSLFHALEKRVHVPGFGSSDQS"
     gene            430..1203
                     /locus_tag="PK171_05295"
     CDS             430..1203
                     /locus_tag="PK171_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOT05635.1"
                     /translation="MTDSKYVLIVEDIHKSFGETEVLRGVSFQVARGETLVFIGPSGT
                     GKSTLLRCVNQLTVPDRGKVWLNGEEVVHSHGKINLYRQKMGMVFQNFYLFDHLTALG
                     NVEISLLKVKKMNKKEAKEKAMHELERVGMVQKANFYPAELSGGQAQRVSIARALAMD
                     PEVMLFDEPTSALDPELTGEVLDVMKNLARAGMTMLVVTHEMGFASSVANQILFLEDG
                     VILERGTPEKIFYRPEFERTQKFLSKMTELYGDLGQKNG"
     gene            1196..1876
                     /locus_tag="PK171_05300"
     CDS             1196..1876
                     /locus_tag="PK171_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOT05636.1"
                     /translation="MDSFVFIQERLLPSLLEGTSVTIKLILLSIPFGLLTGIIVAVGR
                     VYGNKPISMICRLYVLFFKGCPLLLLLFMIYFGLPPIGISLSPMVASVLGFVLCNGAY
                     SSEYIRGAIQSVKIGQITAAEALGMTRFQGVLYIILPQAFRRALPGVGNEIIYLIKYS
                     SLAYMITCIELTGAGKIVAARFFRFTETFMVIGIIYLILVTIATKLLNYAERKLYYPG
                     MGPTSFKG"
BASE COUNT          732 a          503 c          582 g          832 t
ORIGIN      
        1 tttgaatgtt tcagcttttt ttattggtgc actggctttg ggcttaagaa gtgctgctta
       61 tcaaactgaa atatttcgag gagcaatttt gtctctcggc gaaggacaaa tgttagcggc
      121 ccggtcgctg ggcatgagtg aaaaccaagc catatttaat ataatacttc cccaggcact
      181 ccgaatagct ttgccaggat ggtcaaatga atacccaatt ttactcactg attcatcagt
      241 ttgttacgcg ataggtgtca tggaaattat gacccgggga aatcaaatgg ttactcgaac
      301 ctatcaacct atgccaattt attttgcttg tgctttaata tttattttaa tgaattatgg
      361 tggattaagt ctttttcatg cactagaaaa acgggtacat gtccccggtt ttggaagtag
      421 tgatcaatca tgaccgattc aaaatatgtc cttatcgttg aagatatcca taaatctttt
      481 ggagaaactg aagtattgcg tggcgtctcg ttccaagttg ccagaggaga gactctggtt
      541 tttattggac cctcgggaac cggaaaaagc actctgttgc gatgtgttaa tcaattgacc
      601 gttcctgacc gtgggaaagt atggctcaat ggtgaagaag tagtccattc ccatgggaaa
      661 ataaaccttt atcgccaaaa aatggggatg gtttttcaaa atttttactt atttgaccac
      721 cttactgctt tggggaatgt tgaaatatcc ttgctgaaag ttaaaaaaat gaacaaaaaa
      781 gaagccaaag aaaaagccat gcatgagttg gaacgggttg gcatggtaca aaaagctaat
      841 ttttatccag ccgagctatc tggaggacag gctcaaaggg tttctatagc tcgcgccttg
      901 gcaatggatc cggaagtaat gctatttgat gaaccaacct cggctttaga tccggagtta
      961 accggagaag tcctggatgt gatgaaaaat ctggctcgtg ctggaatgac catgctagtt
     1021 gtcactcatg aaatggggtt tgcttcatcg gtagccaatc agattctgtt tttggaagat
     1081 ggagttattc tggaaagagg aacaccggaa aaaatttttt atcggcctga gtttgagcgg
     1141 actcaaaaat ttcttagtaa aatgaccgaa ctttatggtg acctgggtca aaaaaatgga
     1201 tagttttgta tttattcagg aacggctcct tccgtcgctg ttagaaggaa catcggtaac
     1261 catcaagctt attctacttt cgataccttt tggcttgttg accggaataa tcgttgccgt
     1321 agggcgtgta tatggaaata agccgatatc gatgatttgc cgactttacg ttttgttttt
     1381 taaaggttgt cccttattac tactgttgtt tatgatctat tttggtttgc caccaatagg
     1441 aatatcgctc tcgccaatgg tagcatcggt cttgggtttt gtgctgtgca acggggcata
     1501 ttcctcggaa tatatccgag gtgcaattca atcagtaaaa attggccaaa tcacagctgc
     1561 tgaagcctta ggaatgactc gttttcaggg cgttttgtat attattcttc cccaagcttt
     1621 tcgtcgagcg cttcccgggg tgggaaacga gattatctat ttgattaaat attcttcttt
     1681 agcttatatg attacctgca tcgagctgac cggagctgga aaaattgttg ctgctcgttt
     1741 tttccgtttt accgaaacat ttatggttat tgggatcatt tatttaatat tggtaactat
     1801 tgcaacgaaa ttgctcaatt acgctgaaag aaaactctat taccctggca tgggtcctac
     1861 cagttttaag ggttaaaaca aaggtttttt tcttggataa aattggcagt aatatctatt
     1921 taaaacgtcg ttgttagaag catggcgacg tgacaatccc atcctttatc cttttcttta
     1981 aaattttttc ttagaataat caggaaaaac tgaaaggcac cctccccgcc gcaaaacggc
     2041 acccctccaa ggaggggaat tgtggtcttt ccccattgat ggaggactta gtgttgttcc
     2101 taaaatacct cgatagctaa agctcctcat tttactgaaa aaggcaccct ccccgccgca
     2161 aaacagcacc cctccaagga ggggaattgt tgaattcatt cccccattga gggggggtta
     2221 gggggggggt gcctttatat ctttgatggt tatcccgaga aaatgataat gaaatatgaa
     2281 taaaagatga gatcctcacg gcttcaaaag accaagcctc aggatgacaa ctttgatgct
     2341 aaatgagatt accacgttgc acagggcgct cctcgtaatg acggattgag aataggcatg
     2401 atgatgaatc atgtccaata ataaattctt ttctgtgatt acttaatttt tatacctatt
     2461 aatgtatcga cctgccatgg catgtcgata ccttgggttt tcatcctcat ctgagtttac
     2521 aacctggtat ggaggtcttt aatacctttt ctttttttaa taactatgag aaagcaaaga
     2581 gcggttgcca atatgggcat gtattaaccc tttttcttga catctttcca atgctgtttc
     2641 ggcatggtg
//
LOCUS       DAOSOL010000204         2634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_38944_length_2634_cov_2.780147, whole genome shotgun sequence.
ACCESSION   DAOSOL010000204 DAOSOL010000000
VERSION     DAOSOL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38944_length_2634_cov_2.780147"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PK171_05305"
     CDS             <1..1018
                     /locus_tag="PK171_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05637.1"
                     /translation="IRRVLFIPITLFGISLIVFSMIGMLSPYQRLATFISSPDRLKSE
                     NVDQLISMYGLDDPIPIQYGRWFKNLLQGNLGWSYVGKAPVLQALLQRFPYTLELTLF
                     SAIPIILIGTWMGVLSALHHNRTLDHSLRIFAVVGWSFPDFVFGLFILMIFYSTLGLF
                     PPGNLSLWAESIVKSTSFRSFTGMTTIDALLNGRLDVFVNALRHLVGPVLTLTYVQLA
                     YILRITRSSMLDVLQRDYVRTARAKGLDERVVINKHAKRNAMIPVATVSGQTIVGLMA
                     GTVIVEAIFNRTGLGSFLALAAQQLDYASIMGGVLFFGLILIIGNLLVDISYAYIDPR
                     VRLD"
     gene            1023..1904
                     /locus_tag="PK171_05310"
     CDS             1023..1904
                     /locus_tag="PK171_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05638.1"
                     /translation="MKSETHRIIKKLFLNASAIIGFVLLGIFIVIAIFAPFIAPPKEG
                     RDPYMMPIVTWTSSPQPPSPEHLFGVAGKRDIFYGVIWGTRTAFRVGIIVTVVSTLIG
                     LFVGSIAGYYGKLFDEILMRITDIFMAIPFLVAALVLAAILGSGLNKVMIAMTIFGWM
                     YPARLIRGNILQVKEEQYVMAAKALGVKNYLIIIRHVLPNTIFPVLIQASMRMGSLVI
                     TAAALSFLGVGAPQGYSDWGALLNYSRNWMLGGQGEALRYWYSTVFPGAAMVLFVLSW
                     NLVGDALRDVFDPRLRI"
     gene            1915..>2634
                     /locus_tag="PK171_05315"
     CDS             1915..>2634
                     /locus_tag="PK171_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05639.1"
                     /translation="MNTNNPILNIENLKVYFYNDYGQELKAVDDVSFQVYQGETIALV
                     GESGCGKSVTSLALLRLIDSNGKIVGGKINFNNSDLLSLSEKQMQSIRGKDISMIFQE
                     PSSALNPVYTIGDQIIEAISLHQKVSHHEARKRAVEMFKLVGIPDPEKRLNEYPHELS
                     GGMKQRGMIAMALSCHPQLLIADEPTTSLDVTIQAQILELMISLQKQFNMAVILITHD
                     LGIVANMAKRIVVMYAGKVVEE"
BASE COUNT          703 a          529 c          549 g          853 t
ORIGIN      
        1 tatacggaga gttttattta tacctattac tctttttggt atttctctta tcgttttctc
       61 catgattggt atgctcagtc cctatcagcg gttagctacc ttcatcagca gccctgaccg
      121 cttaaaaagt gaaaatgttg atcagctcat ttctatgtac ggcctcgatg atccgatacc
      181 tattcagtac ggaagatggt ttaaaaatct gcttcaaggg aacttaggat ggtcctatgt
      241 tggaaaagca ccggttttac aggctttact ccagcgtttt ccctatacct tggaactgac
      301 gttattttcc gccatcccaa ttatcttgat cggaacctgg atgggagtcc tgtctgcgct
      361 ccaccacaat cgtaccttgg atcattcgct gcggattttt gccgtggtag gatggtcctt
      421 ccctgatttt gtttttggct tattcattct catgattttc tatagcacac tggggctttt
      481 ccctccaggg aatttaagtc tctgggctga aagcattgtt aaatctacca gctttaggtc
      541 ttttaccggt atgacaacta ttgatgcgct tctcaatgga agattggatg tttttgtaaa
      601 tgccttacgg catctcgttg gtccggttct caccttaacc tatgttcaat tggcttacat
      661 tcttcgtatt acccgatcct ccatgcttga cgttctccaa cgggattatg tccgaactgc
      721 gcgggccaaa ggtttagatg aaagggtcgt catcaataaa catgctaaaa gaaacgccat
      781 gataccagtt gctaccgtga gtggccaaac tattgtcgga ttgatggcag gaacggtaat
      841 tgttgaagca atatttaatc gaaccggttt aggaagcttt ttagctttag cagctcaaca
      901 attagattat gcctcgatta tgggtggtgt cctctttttt ggacttatat tgattattgg
      961 gaatcttctg gttgatatct cttatgctta tattgatccc agggttaggt tggactagaa
     1021 aaatgaagtc ggaaacccat cggattataa agaaactatt tttaaacgct tcagcaatta
     1081 ttggctttgt cctcttaggt atatttatcg taatagccat ctttgctcct tttatagctc
     1141 ctcccaaaga ggggagagat ccctatatga tgcccattgt cacttggact tcaagccctc
     1201 aacctccttc accggaacac ctttttgggg tagcagggaa aagagatatt ttttatggag
     1261 taatctgggg tactcgaacc gcttttcgtg ttggaataat agtcactgta gtttcaacgc
     1321 tcattggtct gtttgtcgga tcaatagccg ggtattatgg taaacttttc gatgaaatcc
     1381 tcatgcgaat caccgatatt tttatggcta ttcctttttt agttgcagct ttggtattag
     1441 ctgctatctt aggaagcggg ttgaataaag ttatgattgc catgacgatt ttcggctgga
     1501 tgtacccagc ccgtttaatc cgaggtaata ttcttcaagt gaaagaagaa caatatgtca
     1561 tggcagctaa agcccttgga gtaaaaaatt atttgatcat catccgccat gtccttccca
     1621 atacaatttt tccagtattg atacaagcct caatgcggat gggttcatta gttattacag
     1681 ctgctgccct cagtttcctg ggagttggag ctccacaagg gtattccgat tggggagcac
     1741 ttctcaatta ttctcgaaac tggatgttag gaggacaagg tgaagctcta cgttattggt
     1801 actctactgt ctttcctggt gcagcgatgg ttctttttgt tctatcttgg aacttagtcg
     1861 gggatgccct tcgagatgta tttgatcctc ggctcaggat atgaggagtc aatcatgaat
     1921 accaataacc ctatccttaa tatagaaaat cttaaggtat atttttataa cgattatggt
     1981 caagaactca aagctgtgga tgatgtcagt ttccaagttt accaaggtga aaccattgcc
     2041 ttggttggtg aatcaggatg tggaaaaagt gttacctctt tagccctcct gcgattgatt
     2101 gattcgaatg gaaaaatcgt tggcggaaaa ataaatttca ataattctga tttattatca
     2161 ttatctgaaa aacagatgca aagtatccgt ggaaaagaca tttcaatgat ttttcaagaa
     2221 ccttcatctg cactcaatcc ggtttatacc atcggtgacc aaattattga ggcaatctct
     2281 cttcatcaaa aagtcagcca tcatgaagcc cgaaaacggg cggttgaaat gtttaaactg
     2341 gtaggaattc ccgatccaga aaaacgatta aacgaatatc ctcatgaatt atcaggagga
     2401 atgaaacaaa gaggtatgat tgccatggca ctctcttgcc atcctcagct tttaattgcc
     2461 gatgaaccaa ccacatcttt ggatgtaacc atccaggcac aaattcttga attaatgatc
     2521 agtcttcaaa aacaatttaa tatggcagtg attcttatca cccatgattt aggaattgtc
     2581 gctaatatgg ccaaaagaat tgttgtcatg tacgccggga aagtagttga agaa
//
LOCUS       DAOSOL010000205         2627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_39065_length_2627_cov_4.026439, whole genome shotgun sequence.
ACCESSION   DAOSOL010000205 DAOSOL010000000
VERSION     DAOSOL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39065_length_2627_cov_4.026439"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1690)
                     /locus_tag="PK171_05320"
     CDS             complement(155..1690)
                     /locus_tag="PK171_05320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT05640.1"
                     /translation="MAKGYLLGVDIGTYSSKGVLVQEDGKIIAQKVVPHGMDMPKPGY
                     FEHDADRVWWHDFVEIVKNLLSISGVDPKQILGIGTSAIGSCVLPIDEAGQPLRPAIL
                     YGIDTRTTQEIEYLEKTLGREEIFQHSGMHLSSQASGPKIMWIKNHEPEVFQKTRWFL
                     TSQAYLVYRLTGEATIDIYTANGYAPLFDVHNLGWREEIASYITPIDRLPKAFWSCEI
                     AGRVTEQAARETGLASGTPVIVGTTDAAAEAISTGVREFNDMMLMFGSSIFFIVKTAD
                     LVNTSHFWSAPFLEKDAYTFLGGTSTAGSITTWFRNQFAELELEKEKTEGSNAYDRLA
                     KLAAQSPPGAKGLIILPYFEGERTPLNDPRAKGMFFGISLRHTKADIYRAILEGVGYG
                     IRHNLEVMEEEGVKPRRILATGGGTKNDLWMQIVSDITRVDLSIPKEQIGASYGDAFM
                     AGVGIGLFNNLTEIDHWVQIGKIVQPDLEASRKYDFNYRLFRSLYENTKELMHELSDS
                     LSE"
     gene            complement(1701..>2627)
                     /locus_tag="PK171_05325"
     CDS             complement(1701..>2627)
                     /locus_tag="PK171_05325"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT05641.1"
                     /translation="NLNKIPKKQIAEKLNRSLLKYGSDQQIEWVPEIEAYGMEWADVY
                     IGLRGAHNLKVFWDIPSPKLAQLRRSMGKISTLRWEKTRWCLVRVPNASLAQEAEMDE
                     ETITDMFFNACLLDWSEISEKWNRWATLLSQGSEIRILGYKTDLHFSVKGRSWQVANG
                     KINMPDGEIMTSPDDSTVEGEIYFEFPGVLGGRLMYDVHLQWQKGELVKATSSTHQDF
                     LQSTIQSDPGASRIGEFAFGTNPEVKYFCKDILLDEKIGGTIHIALGRAYPETGGTNK
                     SAIHWDIVKDMRKEGEVYLDGNLIFQKGKMLI"
BASE COUNT          718 a          632 c          510 g          767 t
ORIGIN      
        1 ttcaaattgt aaatccaata gcgggcatga taaatcaagc tcctacaaaa atttttaaaa
       61 atgccgtcat cctgagcggt gctttcccgc gtgaggatct catccatttc tttcctctga
      121 ggattaaggt gagggggaag accagcataa acctctactc ggataaagag tcagataact
      181 catgcataag ttccttggta ttttcataaa gagaacggaa aagacgatag ttaaaatcat
      241 attttctact tgcttctaaa tcaggttgaa caatttttcc gatttgaacc caatggtcaa
      301 tttcagtcag attattaaat aatccgatac ccacccctgc cataaaggca tctccataac
      361 tggcaccgat ttgttcttta gggatagaca aatcaactcg ggttatatct gaaacaatct
      421 gcatccataa gtcattttta gtcccaccac cggtggccaa aatacgtctg ggttttactc
      481 cttcctcttc catgacttcc agattatgtc gaataccata tcccacccct tccaatatag
      541 ctcggtagat atcagccttg gtgtgtctga ggctgatacc aaaaaacatt ccctttgctc
      601 tcggatcatt caaaggtgtt cgttcacctt caaaataggg aagaataata agccccttag
      661 caccgggtgg agactgagct gccaattttg ctaatcggtc gtaggcgtta ctgccttcgg
      721 ttttttcttt ttccagttcc aattcggcaa attggttccg aaaccaggtg gtaatgcttc
      781 cagcggttga agttcctcct aaaaaagtat aagcatcttt ttccagaaaa ggagcgctcc
      841 aaaaatgact ggtattcacc aaatcggctg ttttaacaat aaagaaaata ctacttccaa
      901 acataagcat catatcattg aattctctta ctccagtgct gatggcttca gcggcagcgt
      961 cggttgttcc aacgatgacc ggagtaccgg aagctaagcc ggtttctcga gctgcctgct
     1021 cggtgactcg gccggctatt tcgcagctcc agaaagcctt gggaagtcgg tcaataggtg
     1081 tgatgtaaga agcaatttcc tcccgccaac ctaaattatg gacatcgaac aatggggcat
     1141 aaccgttcgc agtataaata tcaattgtgg cttcaccggt cagtcggtaa acgaggtaag
     1201 cctgactggt gagaaaccat ctggttttct gaaaaacctc cggttcatgg tttttaatcc
     1261 acataatttt aggaccggaa gcctgagaac ttagatgcat tccactatgt tgaaagattt
     1321 cctctctccc caaggttttt tccaaatatt ctatttcctg agtagttcga gtatcgattc
     1381 catagagaat cgccggtctc agcggttggc ctgcttcgtc aataggaaga acacaggaac
     1441 cgatggcact agtccctatt cccaaaattt gttttggatc aacccccgat atgctaagaa
     1501 gatttttaac gatttcaacg aaatcgtgcc accacacccg atcggcatca tgctcgaaat
     1561 aaccgggctt aggcatgtcc attccatgag gaacaacctt ttgagcaatt attttcccat
     1621 cctcttgaac caaaacccct ttggacgaat aagtgccgat atcaactccc agcaaatacc
     1681 ctttggccat aaaattctcc ttagatcaac atttttccct tttggaaaat taagttccca
     1741 tccagataga cttctccctc ttttcgcata tccttcacga tatcccaatg aatggccgat
     1801 ttattagttc ctccggtttc ggggtaagca cgaccaagag caatatgaat tgtcccaccg
     1861 attttttcat caaggagaat atccttgcag aagtacttga cttcggggtt ggttccaaaa
     1921 gcgaattctc cgatccggct ggcgcctggg tcactttgga tggtcgattg gagaaaatct
     1981 tgatgagttg atgaagtcgc tttgaccagc tctccttttt gccattgtag gtgaacgtcg
     2041 tacatgaggc gtccgcccaa cacccccggg aattcaaagt agatctctcc ttcaaccgtt
     2101 gagtcgtcgg gtgaagtcat gatttcacca tctggcatat ttattttgcc gtttgctact
     2161 tgccaactcc gacctttcac cgaaaaatgc aggtcagttt tgtaaccaag tattcgaatc
     2221 tcacttccct gggataaaag tgttgcccag cggttccatt tctcacttat ttccgaccaa
     2281 tctaatagac aagcattaaa aaacatatcg gtaatagttt cctcatccat ttcagcttcc
     2341 tgagccagag aagcattcgg tacccgcact aaacaccagc gagttttttc ccagcgcagg
     2401 gtcgatattt ttcccatcga acggcgtaac tgggcaagct taggtgatgg tatatcccaa
     2461 aataccttca aattatgagc gccgcgcaat ccaatgtata catctgccca ctccattcca
     2521 taggcttcaa tctcgggaac ccattcgatt tgttgatcag aaccatattt gagtaaagaa
     2581 cgatttaatt tttcagctat ttgtttttta ggaattttat ttaaatt
//
LOCUS       DAOSOL010000206         2624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_39116_length_2624_cov_3.952511, whole genome shotgun sequence.
ACCESSION   DAOSOL010000206 DAOSOL010000000
VERSION     DAOSOL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39116_length_2624_cov_3.952511"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PK171_05330"
     CDS             complement(<1..604)
                     /locus_tag="PK171_05330"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT05642.1"
                     /translation="MKEKCIFGPVQSRRLGMSLGINLTPYKTCSLDCAYCECGKTTDL
                     TIIRKDYILIEQVQKTLKRYSGSSLFRSKPPSCVTFAGFGEPTLHSGFGSIAQFAKRT
                     FPDQHLVLITNGTLFPSHPELFQEVQPIDIILPSLDAGSEEIFKKIDRPHPSITLASL
                     VDGLIQLRENYHGQIWLEVFIVEGINDSESELYLMRDAISR"
     gene            complement(649..1203)
                     /locus_tag="PK171_05335"
     CDS             complement(649..1203)
                     /locus_tag="PK171_05335"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOT05643.1"
                     /translation="MFEILVIAFGLGLDAFSLAVAFGMCQKVCTFNTRFRLSLSFGLF
                     QFFMPLAGFYAGLRVARFTESIDHWIVFAVLSFVGGKMIYEALSKDDNETMVDISRGI
                     PLLIASIATSIDALAVGFSFALLKDGILLPAIVIGIVASIMTFVGVTFGHRVGRKYIS
                     KPEVVGGIAIVIIGFKTLLEHFIN"
     gene            1391..2584
                     /locus_tag="PK171_05340"
     CDS             1391..2584
                     /locus_tag="PK171_05340"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOT05644.1"
                     /translation="MKKIIKLITFIGIFIILVNILSSYIYEENIKIKLGNESLFERHF
                     DLIKGKRIGLITNHTGLNSRFQSTAELLANNHQTNLVALFASEHGLDGKAKAGEYVES
                     YLHDQYKIPVYSLYGPTRKPTPKMLENIDLLLYDIQDIGARTYTYISTLNYCMWAAKD
                     NGKTVLVLDRPNPLGGLIVDGPVSEEDFRSFIGVDILPMAHGMTVGELARYFNRRIGV
                     KLIVIPMDGYTRDMMFPDTGLYWIPTSPMIPDFLSALVYMATGLGEGTGIRQGDYFKW
                     VGGKGINSRIFAQELNQIGLPGVQFVPETNGEFGGVRLFITNMRAFQPAYTGLCILSC
                     SHKLISYSVPKNENELTMFDKIMGTALIGRLIENDVSYQDLKKAYWEPLENFKKEREK
                     YLIYQ"
BASE COUNT          852 a          492 c          497 g          783 t
ORIGIN      
        1 tgcggcttat ggcatctcgc ataaggtaga gctcggattc gctatcattg attccttcaa
       61 caatgaatac ctccaaccat atttgtccgt gatagttttc tcgcaattgg attaatccat
      121 cgactaaaga agctaaagtt attgagggat gaggtcgatc aatcttttta aaaatctctt
      181 cgcttccagc gtccaacgaa ggaagaatga tgtctatcgg ttgaacttct tgaaaaagct
      241 cggggtgaga agggaaaagt gttccattag taatgagaac caggtgttga tcaggaaagg
      301 ttctttttgc aaattgagcg attgatccaa atccagaatg aagggttggc tcaccaaatc
      361 cggcgaaagt gacacagctt ggaggtttcg atcgaaacag tgaacttccc gaatatcttt
      421 ttaaagtttt ctgtacctgt tcaataagta tataatcctt acgaattatg gttaaatcag
      481 tggtcttccc acactcacaa taagcacaat cgagactgca agttttatag ggagttaaat
      541 ttataccaag tgacatacct aaacgtcttg attgaacagg accaaaaatg catttttctt
      601 tcattcttct catcgtcctt gtcagcaaat ttttaaagat tggtttcctc agttaataaa
      661 gtgttccaaa agagttttaa acccaattat tacgattgct attccaccaa caacttctgg
      721 tttcgaaata tattttcttc ccacccgatg gccaaaggtt actcccacaa atgtcattat
      781 tgaagcaact ataccgataa caatcgcggg taaaagaatc ccatccttta gcaatgcgaa
      841 agaaaaaccg acagctaagg catcaatact ggtagcaatc gaagctatca gcaaggggat
      901 cccccggcta atatcaacca tagtttcatt atcgtccttg ctcagagctt cataaatcat
      961 ttttccacca acaaagctca ataccgcaaa aacaatccaa tgatcaatag attcagtaaa
     1021 tcgggccact ctaagccccg cataaaaacc agccagaggc ataaaaaact ggaagagacc
     1081 aaaagacaga gataaacgaa atcttgtatt gaaggtacat actttctgac acattccaaa
     1141 agctaccgcc aacgaaaatg catctaggcc aagtccaaaa gcaattacca atatttcaaa
     1201 cacattttct gcctcgtatc gtgcgtgatt tcaattcctt tttttatata attgcgagaa
     1261 ttataacgaa aagtcgagat gaaaaaaaga gcttggaaaa attttttttt gtgtgaaaaa
     1321 agtcggtgtg acaaagcatt aaaaatagct gagtattgtt tatcttactg aaaaaatata
     1381 agataaaaat atgaagaaaa ttataaaatt gattactttt attggtatat tcatcattct
     1441 tgtgaatatt ttatcgtctt atatctatga agagaacatt aaaattaaat tgggaaatga
     1501 aagccttttt gaaagacatt tcgacctcat taaaggaaag cggattggat taataaccaa
     1561 ccataccggg ttgaatagcc gatttcaatc aactgcagaa cttttagcga ataatcatca
     1621 aaccaatttg gtagctttat ttgcttccga acatggattg gacggcaagg ccaaagccgg
     1681 tgaatacgtt gaatcctatc ttcatgatca atataaaatt ccagtttaca gcttgtacgg
     1741 accgacgagg aagccaactc ccaagatgtt agaaaacatc gacctcctcc tttatgatat
     1801 ccaagacatt ggagctcgta cctacacgta tatatctacg cttaattatt gtatgtgggc
     1861 tgccaaggat aatggtaaaa ctgtccttgt tttagaccgt cctaatcctt tgggaggatt
     1921 aatcgttgat ggaccggttt ctgaagagga ctttcggtcc ttcattggcg ttgatatcct
     1981 tcctatggca catggtatga ctgttggtga attagcgcgt tattttaatc gtcgtattgg
     2041 agtgaaattg attgttatac ctatggatgg atatacacgg gatatgatgt ttccggatac
     2101 tggtctttat tggattccca cttcaccaat gattcctgat ttcctttcgg ctttagtata
     2161 catggcgact ggtttgggtg aaggaacggg aattcgacaa ggtgattatt ttaaatgggt
     2221 tggaggaaaa ggtattaatt ccagaatttt cgctcaagaa ttgaatcaaa ttggtctacc
     2281 aggagttcag tttgttcctg aaaccaatgg agaattcggg ggagttcgtc tttttataac
     2341 caatatgaga gcttttcagc ctgcctacac tggcttgtgt attctatctt gttcccacaa
     2401 attgatttcc tattcagtac ctaaaaatga aaatgagtta accatgttcg ataaaataat
     2461 ggggacagcc ctaattggta ggttaataga aaatgatgtt tcctatcaag atttaaaaaa
     2521 agcatactgg gaacctttgg aaaactttaa aaaagagaga gaaaaatatc ttatatatca
     2581 ataacgatcc tgtaaatccg cgggcatgat aaatcaagcc ccta
//
LOCUS       DAOSOL010000207         2617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_39265_length_2617_cov_2.565964, whole genome shotgun sequence.
ACCESSION   DAOSOL010000207 DAOSOL010000000
VERSION     DAOSOL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39265_length_2617_cov_2.565964"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="PK171_05345"
     CDS             complement(<1..986)
                     /locus_tag="PK171_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOT05645.1"
                     /translation="MGVALRSYQEINEKIKKGKVVVVTAEEVIELVDKKGIEKTAREV
                     DVVTTGTFGAMCSSGAMINTGHTKPRMKISEAYLNGVMAYGGLAAVDLYIGATQVAKD
                     DPLNKIYPGEFKYGGGHVIQDLVAGKDILLEAYGYGTDEYPRKKLITWINIKDLNQAY
                     LFNPRNAYQNYNVAVNCSDKIIYTYLGVLLPHLGNAGFSSAGQLSPLLNDPHYRTIGV
                     GTKIFLGGGVGYVAWQGTQHDPGVERNDKGIPKGGAGTLAVIGDMKGMDPNWLWGLSV
                     RGYGCSLAVGLGVPIPVLDEETLQYCAVRDQDIEAPIIDYSNAYPNGTGEVLG"
     misc_binding    complement(1025..1267)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1393..2382)
                     /locus_tag="PK171_05350"
     CDS             complement(1393..2382)
                     /locus_tag="PK171_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05646.1"
                     /translation="MKRFSIILIVTLIFMTFTLPLYAQEKLLGEGMVIYCQMGGAEGA
                     VATIQRTLGAREAAKALGVKLIEQYSQWQPEKMVQQFKEAIAAQPDGIVIMGHPGSAA
                     MWDLVREADSKGIIVTVNNTPLPDIQEEFLAKGFGYAGVDLFAGGYLTGQAMVKAGLK
                     PGDKAIVYGLFSQPFRGQSEEGMARALEDAGIIVDRQEISPEADADASTAIPILTGYF
                     QANPDCKAIGTQHGAITGILVNLLRDNLKKQPGEIISAGIDLSPATIEGLKTGYISAV
                     LDQQLYLQGFIPVVQVVLTKKYKITGFNINTGAGTVTPETIGELEELINAGYR"
BASE COUNT          755 a          644 c          410 g          808 t
ORIGIN      
        1 cccaaaacct cgccagttcc gtttggataa gcgttactat aatcaataat cggcgcttca
       61 atgtcttgat ctcgaactgc acaatactgg agagtttctt catcaagtac aggaataggc
      121 actcccaaac cgaccgcaag agaacatcca taacccctga cacttaaccc ccaaagccaa
      181 ttgggatcca tacctttcat atctccaatc actgccaaag tgcccgcacc ccctttaggt
      241 attcccttat cgtttctttc cactccaggg tcatgctgag tcccctgcca agcaacataa
      301 ccaacgccac cacctaaaaa tatcttggtc ccgacaccaa tagttcgata atgaggatca
      361 ttcaacaatg gacttaactg gccagcggac gaaaagccgg cgttccccaa atgggggaga
      421 agtactccaa gataagtgta gattattttg tcagaacaat tcacggcaac attataattt
      481 tgatatgcat tacggggatt aaagagatag gcttgattta aatctttaat attaatccag
      541 gttattaatt ttttacgagg gtattcgtcg gttccataac cgtaggcctc caacaatata
      601 tctttaccgg caactaaatc ttgaatgaca tgacctccac catacttaaa ttctccgggg
      661 tagattttat tcaatggatc atctttggca acctgtgttg ctccgatata aaggtcaaca
      721 gctgccaaac caccatacgc catcactcca tttaagtaag cttcgctgat tttcatcctt
      781 ggtttggtat gaccggtatt aatcattgca cctgaagaac acatcgcgcc aaaggttcct
      841 gtggttacga catcaacttc tcgagcagtt ttttcaattc cttttttatc aactaattca
      901 ataacctctt cagcagtgac aaccactact tttccttttt tgatcttttc attaatttct
      961 tgataagacc tcaaagcaac tcccatgata tgcgccccct attttaaaaa taaaaaaccc
     1021 ccgcccaagg ggcgggggtt catccgcggt accaccctta tttctgatag tcaaaaacta
     1081 tccaactcat tccccatctc aatcaccaga tggcaatcga ataacgccga ttagacggtt
     1141 ccggctactc tactttcacc gtaacggctc ccgggccatg ttcatcgagt tctgaaggac
     1201 cggcttccac cttacccggt tctctatccc ttcttcccca actactcatc ccgttctaag
     1261 ccgattaata ttttacgaat tataaccagg gtcattataa atgtcaagcg tagtttgttc
     1321 atttaatcaa taatcattct agcttattta aaaaccctct cctaaaaaaa ttggagaggg
     1381 tttttaaatt catcaacgat atccagcgtt aataagctct tccaattcac caatagtttc
     1441 tggagttaca gttccagctc cggtgttaat attaaaaccg gtgattttat actttttagt
     1501 caaaacgact tgaacaacgg ggataaaccc ttgcaaatag agctgttgat caagaaccgc
     1561 actaatgtat ccagttttaa gcccttcaat ggtagctggt gataagtcga taccggcaga
     1621 aataatttca ccaggttgtt tcttcaaatt atccctcaac agattaacca aaattccagt
     1681 gattgctcca tgttgagtcc caattgcctt gcaatccgga ttggcttgaa aatatccggt
     1741 taatattggt atggctgtcg aggcatcagc atcagcttca ggagaaattt cttggcgatc
     1801 aacaatgatt ccagcatctt caagtgctct tgccattcct tcttcgcttt gtccacggaa
     1861 tggctgcgag aaaagaccat atacaatggc cttatctcct ggttttaaac cagcttttac
     1921 catcgcttga ccagttaaat atccacccgc aaacaaatca acaccagcat atccaaaccc
     1981 ttttgctaaa aattcttcct ggatatcggg aagcggtgta ttgttcacag taactataat
     2041 tcccttacta tcagcttctc ttactaagtc ccacattgcc gctgaacctg ggtgtcccat
     2101 aataacgatc ccatcaggtt gagcagcaat agcttcttta aactgttgaa ccattttttc
     2161 cggctgccat tgtgaatatt gttcaataag ctttactcct aatgctttcg cggcttctct
     2221 agcgcctaat gttctctgta tggttgctac tgccccttca gcccctccca tttgacaata
     2281 aatcaccatt ccttccccta aaagcttctc ttgcgcataa agtggaagtg taaaagtcat
     2341 aaatattaat gtaacaatga gaataattga aaaccttttc atttcttttt cctccttaat
     2401 aatattaaac ttataaaata aaaatttgtt ttgtattttc ctaatttaag agctttacct
     2461 cctttctatt taatatttta atgacgtttt catattttca aaaatagatt tattctttct
     2521 ttaacccttt agtaaaaacc gagtttctca aattttcaaa aaaacaaacc cttttcccat
     2581 tggttcgatc taatacaata ttgagcgcaa ttgaacc
//
LOCUS       DAOSOL010000208         2616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_39273_length_2616_cov_4.204608, whole genome shotgun sequence.
ACCESSION   DAOSOL010000208 DAOSOL010000000
VERSION     DAOSOL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39273_length_2616_cov_4.204608"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2066
                     /locus_tag="PK171_05355"
     CDS             <1..2066
                     /locus_tag="PK171_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HOT05647.1"
                     /translation="RKEYLESEKYYITWAIGHLITLAEPEDYEKRFKVWRLSLLPILP
                     DKFFLKPIAKNEKRIKIIKDLLKSDDVEEVINGCDAGREGELIFRYIYEFTESQKPFK
                     RLWLSSMTTAAIRQSFANLIPGERMELLAEAAKCRSESDWLVGINGTRVFTARFKILL
                     SVGRVQTPTLAILVQREKEIREFKPTPYWEIFAVFSSPQGTYVGKWIDGEDRVYDQQQ
                     ALQIEQNVQGKKGKVIEFSNKKTKEQHPLLYDLTELQRDANKIFGFSAKRTLNSAQKL
                     YEARKLITYPRTDSRYLSNDLVEQVTTGWNMLRKCGFEELTQDPFDSKKALKDRRVFD
                     NSRVSDHHAIIPTGEKIDWDTLGRDDQKIMDLICKRFLSVFYPEAIWAQRRIKTEVNN
                     ENFISKSKVLVEPGWRKVYGKEAGSEETEFLPEVEVGTMVTTEKVWVEEKETKAPPRY
                     TEASLLSAMEGAGKFVEDEELQEIMKESGLGTPATRAAIIERLIEVGYLEREEKTLIP
                     SPKGIELINLIESIPVNELASPQLTGQWEKKLVLIEKGQFSRQEFMDEIKKMTEEIVA
                     KVKEKPDNGERAKMNSNVGSCPICGSPVQENRSAFACVQWKEGCPFTLWKKILGKTIT
                     RSQAQKLITQGRTDIISGFKSKKGKFFKARLILQEGGKIGFEFNAPNQKKKDDSVTTL
                     EKKTS"
     gene            2219..>2616
                     /locus_tag="PK171_05360"
     CDS             2219..>2616
                     /locus_tag="PK171_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT05648.1"
                     /translation="MEKFVINGGNPLSGIVEIGGSKNAALPILAATLLSETPSIIRNI
                     PDLLDVNTMILVLQGLGVRIEKLAEGTYQIFPDSLIKSDAPYELIRKMRASFLVTGPL
                     ITRLKRAQVPLPGGCAIGSRPIDLHIKGFL"
BASE COUNT          910 a          431 c          567 g          708 t
ORIGIN      
        1 atcgaaaaga gtacttagaa agcgagaaat attatattac ctgggctata ggtcatctta
       61 ttacattagc tgaaccagaa gattacgaga aaagatttaa ggtttggaga ttaagtcttc
      121 ttcctattct tcctgataag ttttttttaa agccgattgc gaaaaatgag aaaagaataa
      181 aaataatcaa agatctcctt aaatcagatg acgttgaaga agttataaac ggatgtgatg
      241 ccgggcgaga aggagaactt atatttagat atatttatga atttaccgaa agccaaaagc
      301 catttaaaag actttggctt tcatcaatga ctacagctgc aatccgtcaa tcgtttgcta
      361 atcttattcc tggagagaga atggaactat tagcagaagc ggccaaatgt cgatcggaaa
      421 gcgattggtt ggtgggaata aacggaaccc gggtatttac tgctcgcttt aaaattttgt
      481 tgtcggttgg tagggttcag actccaactt tagcgatctt agtccaacga gaaaaagaga
      541 tacgagagtt caaaccaaca ccttattggg aaatatttgc tgtattttct tcaccccaag
      601 ggacttatgt gggaaaatgg attgatggtg aagatcgagt ttacgatcaa caacaagcct
      661 tacaaattga acaaaacgtt caagggaaaa aaggcaaagt gattgagttt tctaataaaa
      721 aaaccaaaga acaacatccc cttctttatg accttactga attgcagcgg gatgcgaata
      781 aaatttttgg tttttctgct aaaagaaccc ttaattcagc tcaaaaactc tatgaagccc
      841 gcaaactgat tacttatcca cgaaccgatt cccgatatct ttctaatgat ttggtcgaac
      901 aggtgacaac aggatggaat atgcttcgaa aatgtggctt tgaagaattg actcaagatc
      961 cttttgactc aaaaaaagca ttaaaggata gaagggtgtt tgataatagc cgagtatccg
     1021 accatcatgc catcattccg accggagaaa aaattgattg ggacactctg ggacgagatg
     1081 accaaaaaat tatggatctg atttgcaagc gatttctttc ggttttttac cctgaggcaa
     1141 tctgggcgca gcgacgaatt aaaactgagg tcaataatga gaattttatt agtaaatcga
     1201 aagtgttagt tgaaccagga tggagaaaag tatacggaaa agaagccgga tcagaagaga
     1261 cggaatttct cccggaagtt gaagttggaa caatggtgac caccgaaaaa gtttgggttg
     1321 aagaaaaaga aaccaaagca ccaccacgtt atactgaagc atctctcctt tctgctatgg
     1381 agggtgcagg aaagtttgtt gaagacgaag agttgcaaga aattatgaaa gaatcggggt
     1441 tgggtacccc cgcaacccga gctgcaatta ttgagcgatt aatcgaggtt ggatatttag
     1501 aaagggaaga gaaaacctta attccttcac ctaaagggat tgaacttatt aatttaattg
     1561 aaagtattcc agttaatgag ttagcatccc ctcaattaac cggccaatgg gagaagaaac
     1621 tcgttttaat cgaaaaggga caattttctc gacaagagtt tatggacgaa atcaaaaaaa
     1681 tgaccgagga gatcgtagcc aaggttaaag aaaaaccaga taatggcgaa cgggcaaaaa
     1741 tgaacagcaa tgttggttct tgtccaatat gtggttcgcc agttcaggaa aaccgaagcg
     1801 cttttgcctg tgttcaatgg aaggaagggt gtccttttac cttatggaaa aaaattttgg
     1861 gaaaaaccat tacccgtagc caggctcaga agctcatcac tcaaggaagg acagatatta
     1921 tttcaggttt taaatccaag aaaggaaaat ttttcaaagc gcggctaatt cttcaagaag
     1981 gtgggaaaat agggtttgaa tttaatgcac caaaccaaaa aaagaaggat gattcggtta
     2041 caactcttga gaaaaaaacc tcatagtaag aaattaaaag agtttaaatt agtgaaaaat
     2101 atgattcaga ataagatata ataatacgag tatctggtta tagaaaaggc aaaaagggat
     2161 acgtaagagg tgcatttttc cggaattaaa aggcgtctgg atgaaaggga gagtaggcgt
     2221 ggaaaagttt gtaattaatg gagggaatcc tttatcaggt atagtagaaa ttggtggttc
     2281 taaaaatgca gcacttccta ttcttgccgc tactctttta agtgagacac cttccattat
     2341 tagaaatatt cctgatttat tagatgtaaa taccatgatt ttggttcttc aaggtttagg
     2401 agtcagaatc gagaagttgg cagaaggaac ctatcaaatt ttccctgact ctctcattaa
     2461 atcagatgct ccctacgaat taatacgaaa aatgagagct tcttttttag tcaccggtcc
     2521 acttatcacc agattaaaaa gagctcaagt tccgcttcca ggtggatgtg caattggttc
     2581 aagacctata gaccttcata taaaaggatt tcttca
//
LOCUS       DAOSOL010000209         2611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_39363_length_2611_cov_4.106025, whole genome shotgun sequence.
ACCESSION   DAOSOL010000209 DAOSOL010000000
VERSION     DAOSOL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39363_length_2611_cov_4.106025"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1769)
                     /locus_tag="PK171_05365"
     CDS             complement(195..1769)
                     /locus_tag="PK171_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT05649.1"
                     /translation="MAETKGPYFLGIDVGTQGLRSGIFDLRGNVVALSFYSYPEYHPR
                     PGWAEQDPIDWWKAVQDTVRRCIYEGEVQPEDIASISVSASSCTVLPVDRFGNPKYRA
                     IMWMDVRAFDQAARINATENPILRYAGGHESPEWMIPKALWLKENLPDIFANSDFIVE
                     CQNWVVFKLTDRWVTSLNNAICKWNYCPTEDGWPISLLRDLNFEEVLQKWPREIIPVG
                     KPVGELTKRAAHELGLIPGIPIIQGGIDAYAAMVGLDVVHPQRLAIVIGSSTCHMALS
                     ETPIFSKGIWGPYQGAVLPDMWILEGGQSSTGSIIKWFSDNFAAQECKEASDIGESLF
                     DVLDKIAAQVAPGSDGLIVLDNFQGNRSPYQDPLARGAVWGLSLSHTKAHVLRAMYEG
                     TAYGTFHILEVLAQDGFDAQEMYASGGGARSKLWLQIHADVTNIPIYLTTVEEASTLG
                     TAIYAAVGAGFYNTIPDATLKMVQISGQIYPNKENHEIYRFYYDKYIKSYFSMKDLMH
                     EISTRVNRNQNSARSH"
     gene            2051..2518
                     /locus_tag="PK171_05370"
     CDS             2051..2518
                     /locus_tag="PK171_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOT05650.1"
                     /translation="MNNIKFIVDCMLGKLARWLRILGFDTQYVRYLTDAQLLAQARYQ
                     NRILLTKDTLLYKKAKNLGYFVHSETIEEQVKEILHHFQLHPHLALTRCPYCNLPLEI
                     PTPTVQIPWTPLYVLRSFSNIKICPRCSQTFWPGTHWKNIIKKLEPLDLSFDE"
BASE COUNT          796 a          575 c          459 g          781 t
ORIGIN      
        1 gattcaatcg atgcctgtaa tcgtttaggt attggtatga tctttactgg aattcgtcac
       61 ttccgacatt aaagtagtga tgggaaatag aggaatacca attaagtgtt cccaattaga
      121 gatttttatc cttaaggaat gaggtaaaac tacctggtgc tttattaaaa aaaggtaaaa
      181 ttccgcccgg aaatttaatg agaccgggcg gaattttgat tgcggttgac acgagtagaa
      241 atttcgtgca tcaaatcttt catcgagaaa taactcttaa tatatttatc atagtaaaac
      301 cgatagattt catggttttc cttatttggg taaatctgtc cagagatttg taccattttc
      361 aaggtcgcat cgggaatggt attataaaaa ccagctccaa ctgcagcgta aatagccgtc
      421 cccaaggtgc tggcttcttc aactgtggtt aaatatatcg ggatattggt tacatcggca
      481 tgtatctgta accaaagttt acttcgggct ccgccaccgc tggcatacat ttcctgagca
      541 tcaaaaccgt cttgggctaa tacttcaaga atatgaaagg tcccataagc cgtcccttca
      601 tacatcgctc gcagcacatg tgctttggta tggctaagag ataaacccca taccgccccg
      661 cgagctaagg ggtcttgata agggctacga ttcccctgga aattatcaag aactatcaat
      721 ccatcagaac caggtgctac ctgagcagca attttatcca atacgtcaaa caggctttct
      781 ccaatatcac tggcctcttt acattcttga gcagcaaaat tatcactaaa ccatttaata
      841 atcgaaccag tagaactttg accgccttct aaaatccaca tatcgggaag aaccgctccc
      901 tggtaaggac cccaaattcc tttactgaat attggtgttt ctgataaagc catgtgacag
      961 gtagaggaac cgatcactat tgctaagcgt tgaggatgga cgacatctaa ccctaccata
     1021 gctgcataag catcaatacc gccttgtata ataggaatcc ctggtatgag accaagttca
     1081 tgggcggctc gtttggttaa ttcacccact ggttttccca caggaattat ctctcgaggc
     1141 catttttgga gaacttcttc aaaatttaaa tctcttaaaa gggatattgg ccatccatct
     1201 tcggttggac aatagttcca cttacaaatg gcattgttca gcgatgtcac ccaacgatca
     1261 gtgagtttaa aaactaccca gttttgacac tcaacgataa aatcagaatt agcaaagata
     1321 tccggtaagt tctccttaag ccaaagagcc ttgggaatca tccattccgg tgattcatgg
     1381 ccacccgcat atcttaaaat tggattttca gtggcattaa tcctcgctgc ctgatcaaat
     1441 gctctcacat ccatccacat aatggctcgg tatttaggat ttccaaagcg atctacgggc
     1501 aatacagtac aagaactggc actcacgctg atcgatgcaa tatcctccgg ttggacctct
     1561 ccctcataga tacaacgtcg aacggtgtcc tggactgctt tccaccaatc tattggatct
     1621 tgttctgccc atcccggacg gggatggtat tcgggataag agtaaaaaga gagggcaaca
     1681 acatttcctc tcaaatcaaa aattccactt ctcaaacctt gtgttccgac atcaatgcca
     1741 agaaaataag gccccttggt ttctgccata atatgaccct ccggatatcc aatctataaa
     1801 aaacccgtga ataaatacct cgtccttatc cgtattaagg ttcttgaatc cttttaccaa
     1861 ttatgacaca aatagaatga gattgaacac ttttttggat tttttatgat ccctagtcca
     1921 taccaatact ttccaattct tgatattatt atacataata ataaaatcaa tttttctttt
     1981 ttttattgga atttaaagaa agttaaatcc caatgactta atggtaagtc gcaaaatctg
     2041 gtaggaataa atgaataata tcaaatttat tgttgattgt atgttaggaa agttagcccg
     2101 ctggttaagg attcttggct ttgataccca atatgttcgt tatctcactg atgcacaatt
     2161 actggctcaa gcccgatatc aaaaccggat tttattaacc aaagacaccc tgctgtataa
     2221 aaaagctaaa aaccttgggt attttgtcca ctcagaaacc atcgaagaac aagtaaagga
     2281 aatactgcat cacttccaac tgcaccctca tctggcttta actcgttgtc catattgcaa
     2341 cctaccatta gaaatcccta ctccaacggt tcaaattcct tggacacctc tctatgttct
     2401 tcgcagtttt tctaatatta aaatatgccc tcggtgtagc caaacttttt ggcctggaac
     2461 tcattggaag aatataataa aaaaattaga gccccttgat ctttctttcg atgaataaat
     2521 aagcccccca aatatctttt tgtaaattta gggggcttat taaataaaat tctattaatt
     2581 tttaaaacct tatcctaaaa ttacttgctc t
//
LOCUS       DAOSOL010000210         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_40106_length_2575_cov_3.398016, whole genome shotgun sequence.
ACCESSION   DAOSOL010000210 DAOSOL010000000
VERSION     DAOSOL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40106_length_2575_cov_3.398016"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..2408
                     /locus_tag="PK171_05375"
     CDS             162..2408
                     /locus_tag="PK171_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT05651.1"
                     /translation="MSEQYDLIVVGGGLAGTCAAVAAARLGCRVALVQNRPVLGGNSS
                     SEIRVPVGGACDFNPWARETGLLEEFFLEDRFQDPARIWTGHGSSTWDYILYKAAANE
                     KNLDLYIDTPAQKVLMSETNPKFIDSIVSYENGSEKEIVLQGRLFIDATGDGVIAYQA
                     GAEFRMGREARSEFEESLAPEKADSYTQGSSLLFHAKDVGFPVPFTPPEWVPQLTSDE
                     DLAFRTHHDVEAGYWWIEIGNPPYNTITDHANIRKELIKMVLAVWNHIKNHGDHQADH
                     LVLDWIGMVPGKRESRRIMGDYIMKEKDVTYGSLFDDRVAYGGWFVDIHTPGGILAKD
                     LPPEPSFPGKSEEIGKRQATVYSIPFRSLYSKDIPNLMMAGRDISVTHVALGTTRLMG
                     TCSTIGQATGAAAYLCTKYSLQPRDLYPEKIHELQQLLLKQDGFIPQIKNSDPKDLAR
                     DARITASSSAKLQIAQGDLATEYDHPRQRTISMTGLETERALIFPITSDHIDSIDLYI
                     ESHLHESAEIEVTLKKAMHIWDYDGSEGILTKQKVAVPPSGVSWVKVPFNLSVDPKSF
                     YIITARSQTGIFWRYHKKPPVGTAALSKEKNKWTSTKGAYTIRIYPEVFPYETENILS
                     GVSRPENWTNIWISDPSQGMPQTVTLEFPVETTFNTVYLTFDTNLTQTHMSTPPLYLF
                     PECVKDYALFFDAQATWKEILHCHDNYQRRRIHRFSPITTQKLQIEIYATHGDPSARI
                     YEIRVYNE"
BASE COUNT          767 a          570 c          535 g          703 t
ORIGIN      
        1 atagggattt tcatcatcat ctggtgttgc aaagcaacat ggatgtctat ccttttgtat
       61 ttttttattt tttaattttt ttcaaacgct tagacagcaa ttacggtttt tgcaaagcaa
      121 aaattataat attgtaaaga agaatgacta aggaggtttt catgagcgaa caatatgatc
      181 tcatcgttgt aggaggaggt ttagccggaa cctgtgctgc tgttgctgct gcacgcttgg
      241 gatgccgagt tgccttggtc caaaatcgtc cagtacttgg cggaaattcc agcagcgaaa
      301 ttcgcgttcc agttggagga gcttgcgatt tcaacccttg ggcaagagaa actggcctgc
      361 ttgaagagtt ttttttagag gatcgctttc aggatcctgc acgaatctgg accggtcacg
      421 gatcttcaac ctgggactat atcttatata aagcagcggc caatgaaaaa aatcttgacc
      481 tttatatcga tacaccagct cagaaagttc tcatgtcaga aaccaatcca aaattcattg
      541 attcaatcgt aagttacgaa aacggatcag aaaaagagat cgttctccaa ggtcgtttgt
      601 ttatcgatgc caccggagat ggagtgatcg cctaccaggc gggagcggaa ttccggatgg
      661 ggagggaagc gagaagtgaa tttgaggaga gtttagcacc agaaaaagcc gacagttata
      721 ctcaggggag tagcttgctt ttccatgcca aagatgtcgg tttcccggta ccctttaccc
      781 caccagagtg ggtcccccaa ctcactagtg atgaagacct ggcttttcgc acccatcatg
      841 atgtcgaagc cggttattgg tggatcgaaa ttggaaatcc tccctataac actataacag
      901 atcacgcaaa tatccgtaag gaactcatta aaatggtcct ggcggtttgg aaccacatta
      961 aaaatcacgg tgaccatcaa gccgaccact tggttctcga ttggattgga atggtccctg
     1021 ggaaacgaga gtcgagaagg attatgggtg attatattat gaaagaaaaa gatgtcactt
     1081 atggatcttt atttgatgat cgagtagcct acgggggatg gtttgtcgat attcacactc
     1141 caggaggcat tcttgcaaaa gatttacctc ctgaaccatc atttccagga aaatcagaag
     1201 aaattgggaa acggcaagct accgtctaca gtattccatt tcgatcgctt tattccaaag
     1261 acattcccaa cctgatgatg gcaggaagag atattagtgt tacccatgtt gctctgggaa
     1321 ccactcgttt gatgggaact tgttctacca ttggtcaggc aactggcgct gctgcttatt
     1381 tgtgtaccaa atattctctt caaccccggg acctttatcc agagaaaatt cacgaactcc
     1441 aacagctcct tctaaaacag gacggtttca tccctcaaat aaagaatagc gatccgaaag
     1501 acttggctcg ggatgcccga atcaccgcat caagttcagc caagctccaa atagcccaag
     1561 gagacttggc gaccgagtat gatcatcctc gacagcggac aatatcaatg accggccttg
     1621 aaaccgaaag agccctgatt tttccaataa cttcagatca cattgactca atcgatctct
     1681 atattgaatc tcatcttcac gaatcagctg aaatagaggt tactcttaaa aaagccatgc
     1741 atatttggga ttacgatgga tcggagggaa ttctcacaaa acaaaaagtg gctgtgcctc
     1801 ccagtggtgt ttcctgggtg aaagttccct tcaacctttc agtagatcct aaatcttttt
     1861 acattatcac cgcccgttcg caaaccggta tcttttggag atatcataaa aaaccaccgg
     1921 taggaacggc agccttatca aaagaaaaaa ataaatggac ttcaaccaaa ggagcttata
     1981 ccattaggat ttatcccgaa gtattcccct atgaaactga aaatatcctc agtggagtga
     2041 gccgtcctga aaactggacc aatatctgga tttccgatcc ctctcaaggt atgccacaaa
     2101 cagtaacgtt ggaatttcca gtcgaaacca cattcaatac ggtttatctg actttcgaca
     2161 ccaatcttac ccaaacccat atgtccacgc cgcctctcta tctttttcct gagtgtgtca
     2221 aagactatgc cttattcttt gatgctcaag ctacctggaa ggaaatcctc cattgccatg
     2281 acaattacca aagaagaaga atccatcggt tttcacctat tacaactcaa aaactccaaa
     2341 tcgagatcta cgctacccat ggagaccctt cagcgcggat ttacgaaatt cgggtgtaca
     2401 acgaataagt taatttattt tatggaggcg ttttcaaaag ttatcaaaat aatgatttaa
     2461 attgcataaa tttaattcgt ataatgcgtg cccggcggag aaaaatcagg cacgcattat
     2521 ttttataaaa ttgccccttg ttcttttaaa gttctctgta aaacatctac cggaa
//
LOCUS       DAOSOL010000211         2572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_40170_length_2572_cov_3.530393, whole genome shotgun sequence.
ACCESSION   DAOSOL010000211 DAOSOL010000000
VERSION     DAOSOL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40170_length_2572_cov_3.530393"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="dapG"
                     /locus_tag="PK171_05380"
     CDS             <1..1176
                     /gene="dapG"
                     /locus_tag="PK171_05380"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT05652.1"
                     /translation="LREKAALKLKEAYEEGYSPVAVVSAMGRAGAPYATDTLISIAKT
                     EYEHLDSRNLDILMSTGEIISTVIMTQALRKLGLKAVAVTGWQAGIITDDRFGDATIK
                     RVETKKILSYLEKGYIAVVAGFQGASEDGEITTLGRGGSDTSAVILGVALEAEYVDIF
                     TDVIGVMTADPRIVPEAHILEGLTYTEAAEIIQQGAKVIHDKAMNIAREYRVPLRVRS
                     LNDEGKGTLICDIRSMSKEGLRLFSQDRTVYSIAFRKGLAQFRVKLNGKKEDNLLMFQ
                     LLANKGISIDLINLFPDIVAFVVEEGKALRVKEILEKEGFELTLIYPCAKISLVGSGM
                     ADRPGVVADMVEALRSSKIEILQTSDSHVTISCLVHATDMEKAIQVLHRKFQLEDIC"
     gene            1252..2139
                     /gene="dapA"
                     /locus_tag="PK171_05385"
     CDS             1252..2139
                     /gene="dapA"
                     /locus_tag="PK171_05385"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT05653.1"
                     /translation="MECDFGQMLTAVVTPFDSSLAVNYDQFRKLLNYLVKEGSDGIVV
                     SGTTGEAPTLTKDEKLKLFEIALEEVGDRAKIIAGTCSYDTHESVFLSQEAEKIGVHG
                     ILAVAPYYNKPPQEGLYQHFRAIAEGISIPVMLYNIPSRTGINIEPETIARLSEIPNI
                     CALKEAAGSADQLSVLRTLLPKNFAIYSGDDNMTLVVLALGGKGVVSVASHVAAREIK
                     NMIVLYKQGKVEEALHLHLRLYSLFKIIFISTNPIPIKAALNLMGWSVGKVRLPLSQM
                     EKEKEDKLKKTLLDLGYLS"
BASE COUNT          806 a          431 c          596 g          739 t
ORIGIN      
        1 ttacgggaaa aggctgcatt aaagctaaaa gaagcttatg aagaaggtta ttcgccagtg
       61 gctgtggtct cagcgatggg gcgagctggg gcaccttacg ctactgatac tctgatttct
      121 atagccaaga ctgaatatga acatttagat tcaagaaatt tagatatctt aatgtcgacc
      181 ggagaaatta tttcgacggt gattatgacc caagctctca gaaaattggg tttaaaagca
      241 gttgcagtta ccgggtggca agctggaatt ataactgatg atcgttttgg tgatgctacc
      301 atcaaacgtg tcgaaaccaa aaaaatattg agttacttag aaaaagggta tattgcagtg
      361 gtagcgggat ttcagggggc atctgaagat ggagaaatca cgactttagg tcgaggagga
      421 agcgatacct cagcagtaat tttgggagta gctttagaag cggaatatgt tgatattttt
      481 accgatgtta ttggagtaat gaccgctgat cctcgcattg ttcccgaagc ccatatctta
      541 gagggactga cttatactga agctgctgaa atcattcaac agggagcgaa agtaatccac
      601 gataaggcca tgaatattgc ccgggaatat cgggttccac tgcgggttcg gagtttaaat
      661 gatgaaggga aaggaacttt aatctgcgat attcgctcga tgtcaaaaga gggtttgcga
      721 ttgttttcgc aagatagaac agtttacagt atagctttcc gaaaaggatt ggctcaattt
      781 cgagtaaagc ttaatggaaa aaaagaggat aatttattga tgtttcaact cttagccaat
      841 aagggaatta gtatcgacct catcaatctc tttcccgata tcgtggcctt tgtcgttgaa
      901 gaaggaaaag ccttaagagt aaaagaaata ttggaaaagg agggttttga gcttaccctc
      961 atatatccct gcgcaaaaat atccttggtt ggttcgggga tggcggatcg tcccggagtc
     1021 gtggccgaca tggttgaagc cctgagaagt tccaaaattg aaatcctcca aaccagtgat
     1081 tcccatgtga caatttcttg tctggttcac gctaccgata tggaaaaagc gattcaggtt
     1141 ctccatcgaa aatttcaact ggaagatatt tgttgaatat atcagtgaaa ttgaaagaaa
     1201 aaaagacatt ggtagaatcg cttatgagaa aatttcttga ggaggttcat catggaatgt
     1261 gattttggtc aaatgttaac tgcagtggtg actccgtttg attcaagtct tgcggtgaat
     1321 tatgatcaat ttcgaaaatt gttaaattat ttagtaaaag agggatcaga tggaattgtg
     1381 gtttccggaa ccactggaga agcaccaaca ttaaccaagg atgaaaagtt aaaacttttt
     1441 gagattgcct tagaagaagt gggagatcgg gccaaaatta ttgccggaac ttgtagctat
     1501 gatacccacg aatcagtgtt tttgtcccaa gaagcggaaa aaatcggggt tcatggaatt
     1561 ttagctgttg ctccttacta taacaaaccg ccacaagaag gtctttatca acacttccga
     1621 gctattgccg aaggaatttc cattccggtt atgctttaca atataccttc tcgaaccgga
     1681 attaatatag aacccgaaac aatcgctcga ctctctgaga tcccaaatat ttgtgccctg
     1741 aaagaagcag ctgggagcgc tgatcagttg tcagttttgc gaactctgct tccgaaaaat
     1801 tttgcgattt atagtggcga tgataatatg accttagtgg tattagcttt gggaggtaaa
     1861 ggagttgtca gcgtagcctc tcatgtggca gcccgtgaaa ttaagaatat gattgttctt
     1921 tataaacaag gaaaagttga agaagcactt cacttgcatc ttcgtttata ttctcttttt
     1981 aaaataatat ttatttcaac caatccgata ccaattaaag ccgcactgaa tctaatgggc
     2041 tggtcagttg gaaaagttcg gcttcctctt agccagatgg agaaagaaaa agaagacaag
     2101 ttgaaaaaaa ccttactcga tcttggatat ttgagttgaa atttcctttg aattttgtca
     2161 taatagttga accttaaaaa agaatatgaa aacagcaatg atcgggaagt tcattggaaa
     2221 aattatagcg aggaagtttt atggtgaaag gcttccaatt atttccgata gaacgagagc
     2281 ccgtgagctg atcctgaaga aaaggctttt taagtctaag taggggaaat ccggtcaaac
     2341 cgttaggaaa atgagtagaa cagggtggca ccgtgtacct attggttcac ccctgtagtt
     2401 ctgttcgaga aaacctcgtc tatgggacga ggttttttgt ttttggaaga ataaatcgtt
     2461 gtttttaagg ttaaaaattt ctcgagaatg atgaaaaaag ttatctaaat ccgtcattca
     2521 gactgtgtca caaatccttt tttcatcaag aataatgtga aagtggagga ca
//
LOCUS       DAOSOL010000212         2546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_40728_length_2546_cov_4.101566, whole genome shotgun sequence.
ACCESSION   DAOSOL010000212 DAOSOL010000000
VERSION     DAOSOL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40728_length_2546_cov_4.101566"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(418..1266)
                     /locus_tag="PK171_05390"
     CDS             complement(418..1266)
                     /locus_tag="PK171_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT05654.1"
                     /translation="MKIAVASGKGGTGKTLIATSLAKVISRSKSVIFLDADVEAPNAH
                     FFLKPDFYENQIVTSFTPLVIDDRCDGCGKCVEFCAYSALALVNKKILFFPELCHSCQ
                     GCRVVCPQAAIEKDESVKGTIDKGKTSDGIDFRRGILNIGEPRSPVVINVLKKNLPQE
                     PNQVIIIDCPPGTGCALIAAVEGTDYCLLVTEPTPFGFSDLKMAVAAIEELHIPFGVI
                     INKQGLDGNKTEVYCREQGIDILGRIPFERSIGERYAQGMTLVDGENNYVHDLLAIFK
                     KIGTQS"
     gene            complement(1263..1466)
                     /locus_tag="PK171_05395"
     CDS             complement(1263..1466)
                     /locus_tag="PK171_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05655.1"
                     /translation="MRGLGNRSSGGGGRGMGRGGGAGAGPGGSCVCPQCGTKVPHQPG
                     IPCFQVKCPQCGTNMVREGITPS"
     gene            complement(1487..1852)
                     /locus_tag="PK171_05400"
     CDS             complement(1487..1852)
                     /locus_tag="PK171_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT05656.1"
                     /translation="MKIVITSKGKTLKDDLDPRFGRCQYFLIVDNETNEVEAIPNPQV
                     SAGGGAGIQTAQFMVDRGVKAVLTGNMGPNASKVLNEAGIKVYINNSGTGESVLNDFK
                     NGKLTPTTQANVSSHFGLK"
     gene            complement(2051..2425)
                     /locus_tag="PK171_05405"
     CDS             complement(2051..2425)
                     /locus_tag="PK171_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT05657.1"
                     /translation="MKRIAISTDGDQVSPHFGRCTSYTIIDLEDGKVLAQEVIENPGH
                     EPGFLPQYLHKGGVNAIVAGGMGPRAQNLFQEVGIDVYLGAEGPIDAVIEQLQKGTLI
                     AGESSCTHEHGSGNGDCDHHHG"
BASE COUNT          690 a          626 c          427 g          803 t
ORIGIN      
        1 gatgttttac cggttccacc cttcccgctt agtattgcaa tttgtttcat agttaaaacc
       61 tctctaagca caatagttta tttttatttc gaaaacccca cgagcttgat aaatcaagtc
      121 cctaccaaag aattttaaaa aggtgccatc cggattggtg ctttcccgcg tgaggatctc
      181 atcttgaaat tttttctttt atcccttaat tcttttttca ttcgctccct ctcccattct
      241 cccccctcgc cccctcagcc tttatcgtgc ccgattaatg tagtgacatg ccgtggtgtg
      301 tcgaatcttg gacttgcacc ctcatcctga ccttctccca tttttttctt ttttcctctc
      361 ccctggtggg agaggattaa ggtgaggggg gaaacttggt caagatagaa acgaagccta
      421 actctgagtt ccgatctttt taaaaatagc aagaagatca tgtacataat tattttcacc
      481 atctacgagg gtcatacctt gggcatatct ttcacctatc gatctttcaa atggtatcct
      541 ccctaaaata tctataccct gttctcgaca ataaacctcg gttttatttc cgtctaaacc
      601 ctgtttatta attatgactc caaatggaat atgaagctct tcgatagcgg caactgccat
      661 tttaagatcc gagaagccaa aaggtgtagg ctcagtaacc aacaaacaat agtcagtacc
      721 ttcaacagct gctatgagtg cacacccggt tccaggtgga caatcgataa taataacttg
      781 attcggttct tggggaagat tcttttttaa aacattaata accacaggag atcttggctc
      841 cccaatattc agaattcctc gtcgaaaatc aataccatcc gaggttttcc ccttatcaat
      901 agtacctttt acggattcat ctttttcaat agctgcttga ggacaaacaa cccgacatcc
      961 ttgacaacta tggcaaagct ccgggaaaaa aagtattttc ttgttcacca aagccaacgc
     1021 actataagca caaaattcaa cacattttcc acaaccatcg catcgatcat ctatcaccaa
     1081 aggagtaaac gaggtgacga tttggttctc ataaaagtcg ggttttaaaa aaaaatgagc
     1141 gtttggtgct tctacgtctg catcaagaaa tatcaccgat tttgatcgtg aaataacttt
     1201 tgccaaactc gtcgcgatga gggttttccc agttcctcct tttccactgg ctaccgcaat
     1261 tttcatgatg gtgttatacc ttcacgaacc atatttgtcc cacattgagg acacttaacc
     1321 tgaaaacagg ggatgcctgg ttgatgtgga actttggttc cgcattgtgg acatacgcag
     1381 ctcccacccg ggccggcacc tgctccacct cctcgtccca ttcctcttcc tccaccacct
     1441 gaagatcgat ttcctaatcc acgcatcgtt ttatcctcct tttcaactat ttcaaaccaa
     1501 aatgagaaga aacatttgct tgagttgtag gagtcaactt tccattttta aaatcattga
     1561 gtaccgattc tcctgtcccg ctattattaa tatagacttt aattcctgct tcattcagaa
     1621 cttttgatgc attggggccc atatttcctg ttaataccgc tttaactcct cgatccacca
     1681 taaattgagc ggtttgaatt ccagctccac cccccgccga aacctgtgga ttgggaatgg
     1741 cttccacctc attagtttca ttatcaacaa ttaaaaaata ttggcatcgg ccaaatctgg
     1801 gatcgaggtc atctttaagt gtctttcctt tagaagtaat tacgattttc attgtgtatc
     1861 catccttcct taatcaattt gtgaaaagga atttactctc acacttgccc aattaacttt
     1921 tccatatcgg tgataagaga gctactcctt tctcttttca actataatgg gaaaagatca
     1981 gcctctcttc tcaattcttc aatgtcaccg ctatttataa attcaagaat caagacctaa
     2041 ccccgggagc ttaaccgtgg tgatgatcac aatctccatt tcctgaacca tgttcatgag
     2101 tacaggaact ttcgccagca atgagagtcc ctttctgtaa ttgttcaata actgcatcaa
     2161 ttggtccttc tgcaccaaga tagacatcaa tccctacttc ttgaaagaga ttttgtgccc
     2221 gagggcccat gcctccggct acgatcgcat taactccacc tttatgaaga tattgaggca
     2281 gaaatcctgg ttcatgaccg ggattctcaa taacttcttg agctaaaact ttcccatctt
     2341 ctaaatcaat aatagtatag gaagtgcaac gaccaaaatg aggtgatacc tggtctccat
     2401 cggttgatat cgcaattctt ttcattaaaa aacctccaat actagggtat gaagagagag
     2461 ggagacccct ctctcctttt tattctatat attatctttt ggggcgcttt tctgaagttc
     2521 ttcaattcgc ttatcgattt gttcca
//
LOCUS       DAOSOL010000213         2523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_41207_length_2523_cov_4.509319, whole genome shotgun sequence.
ACCESSION   DAOSOL010000213 DAOSOL010000000
VERSION     DAOSOL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41207_length_2523_cov_4.509319"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1725)
                     /locus_tag="PK171_05410"
     CDS             complement(<1..1725)
                     /locus_tag="PK171_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT05658.1"
                     /translation="MKRVENKEFLELISDSRPAQQEAIMADEDLVVVSAGAGTGKTET
                     LARRYAWLVTTGRAKFNQILTLTFTEKAALEMRERIRMRLRNWYKQLSNQGNFQHLYE
                     AIDNLDEAPISTIHSFCMRIIQKASLYFDIDPNIRVISSPEEFGFWNNLQNILRRNDW
                     NWFDENVSDPVWQSRLSILNQEPFFLLLNHGGADDFVQLVNSLSDLFGSRGENPEWLW
                     QKGERVERDWEEIQESLLSLYATQWDEEWSLWIELILPLMDLRGSSKVIGTFIDFNLK
                     WHNRPDKRQLPFFMSDLIDSLTAIAKSSRNAVYLEIQDQLLLSRPFQSMIEYRNQLKE
                     KYLHLIDILCGKVELSEDFRCRQFLIQSSAIFWQVWEEFKKKKSLLTFNDMIAYAGEA
                     IKQYPTLTSQYSSIMVDEFQDTDLLQESLIRNIVQNNQASLFVVGDLKQSIYRFRHAD
                     LSIFHQYIQETQYNSKSGRHIVLGESFRSRDDLVSEMNSLFGFIWKDGLSQKIPHEYD
                     PLQVPNELSWWEERSKSVSASHLRFLFLGNTDQTTKETERRCLLARYLADEVNNLVGK
                     AIVWDKAEK"
     gene            complement(1712..>2523)
                     /locus_tag="PK171_05415"
     CDS             complement(1712..>2523)
                     /locus_tag="PK171_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT05659.1"
                     /translation="VECPFLFYLKNFYKLTEPTPPGFDPRRGGIVLHELWRKVWDNYL
                     EGKSTSLRELVLSLWTEIVQRVYPELLTVFTSDELRLRLDALRMADYQEHMESFINYY
                     RTEEKSEFVLPLYEINQLSFTGRCDRLIGLKDGYWLIVDYKSGKSNYARKSLQLAGYS
                     QVLTDNGYPVIGYVYLCHGDGKATGAFENTLFVPYGDKLRNAILKSNRSGKILSVEME
                     LARQKMEELSLGLASGDIQPNYHSQTCSGCFGRSLCRRDEMRSRGGEDEES"
BASE COUNT          737 a          567 c          465 g          754 t
ORIGIN      
        1 tttttctgct ttatcccaaa ctattgcttt ccctaccaag ttattgacct catctgctaa
       61 gtatcgtgcc agaagacaac gcctttcggt ttctttggtc gtctgatcgg tgttccctag
      121 gaagagaaac cgcagatgag aagccgatac cgattttgag cgctcttccc accaggagag
      181 ctcattggga acctggagtg gatcatactc atggggaatt ttttgactta atccatcttt
      241 ccagataaaa ccaaataaac tgttcatttc actaaccaaa tcatctcgac tacgaaagct
      301 ttcaccaagc acgatatgcc ttccactctt tgaattatat tgagtttctt gaatatattg
      361 atggaaaata ctcaggtcgg catgtcgaaa acgatatatg gactgcttca aatccccaac
      421 cacaaagaga gaggcctggt tgttttggac aatattacga atcaaggatt cctggaggag
      481 atcggtgtct tgaaactcat caaccataat agagctatat tgggaagtta aagtgggata
      541 ctgttttatt gcctcaccgg cataggcaat catatcatta aaggtcagca ggcttttttt
      601 cttcttaaat tcctcccaaa cctgccaaaa aatagctgat gattgaatga gaaattgacg
      661 acaacgaaag tcttcggaca attcaacttt cccacataaa atgtcaatta aatgaaggta
      721 cttctctttt aattgattgc ggtattcaat catagactgg aaaggtcttg atagtaaaag
      781 ctgatcttgg atctccagat atactgcatt ccttgagctc ttggcaatgg cagtcagaga
      841 gtcaattaaa tcactcataa aaaatggcag ttgtctcttg tctggtcggt tatgccactt
      901 tagattaaaa tcgataaagg tcccaattac tttgctcgat ccgcgtaaat ccattaaagg
      961 taaaataagc tctatccaca gcgaccattc ctcgtcccat tgagttgcat acaaggatag
     1021 gaggctttct tggatttctt cccaatccct ttcaactctt tcaccttttt gccaaagcca
     1081 ttctggattt tctccccgac ttccaaaaag gtcggaaaga ctattaacca attgaacgaa
     1141 atcatcagca ccaccatggt ttaaaaggag aaaaaatggt tcttggttta atatcgacaa
     1201 acgactttgc cataccggat cagaaacgtt ttcatcaaac caattccagt catttcgtct
     1261 taaaatattt tgaagattat tccaaaaccc gaattcttcc ggtgatgaaa tgactcggat
     1321 atttggatca atatcgaaat acaaacttgc cttttgaatt attctcatac aaaaagaatg
     1381 aatggtggat ataggagctt catcgaggtt atcaattgct tcgtaaagat gttgaaagtt
     1441 tccctgatta gataactgct tataccagtt ccgcagtcgc attcgaattc tctctctcat
     1501 ttcaagggcg gctttttcgg taaaggttaa agtcaggatt tgattgaatt ttgctcgtcc
     1561 agtggtgacc agccaggcat agcggcgagc taaggtttcg gtttttccgg tgccagcacc
     1621 agcgctcacc accaccaaat cttcatcagc catgattgct tcctgttgag ccggtcgaga
     1681 atcggaaata agctccagga attctttatt ttcaactctc ttcatcttct cctccacgac
     1741 ttcgcatctc gtctcttcgg caaagtgatc tcccaaaaca tccactacaa gtttggctat
     1801 gataatttgg ttggatatca ccgcttgcca aacccaagga gagttcttcc attttttgtc
     1861 gagcaagttc catctcgacg cttaaaattt taccggaacg attcgacttt aagattgcat
     1921 tcctcaactt atcaccatag ggaacaaaaa gggtattttc aaaagcacca gttgctttac
     1981 cgtcaccatg gcagaggtat acatagccaa tcaccggata tccattatcg gttaaaactt
     2041 ggctgtatcc ggccaactgc aacgatttcc ttgcgtaatt tgatttccca cttttataat
     2101 ctacaatcag ccagtatccg tcttttaaac caatcagacg gtcgcaacgg ccagtaaaag
     2161 aaagttggtt tatttcatac agagggagaa caaattcact tttttcttcc gtacggtaat
     2221 aatttataaa actttccata tgttcctggt agtcggccat tcttaaagca tcaagcctca
     2281 agcgaagttc atcgctagta aaaaccgtta aaagctctgg gtagacccgt tggacaattt
     2341 ccgtccaaag ggacaacacc agttcccgaa ggctggttga ttttccttct aaatagttat
     2401 cccacacttt tctccacaat tcatgcaaaa ctattccacc ccgacgggga tcaaaaccgg
     2461 gaggagttgg ttcggttaat ttatagaaat ttttgaggta aaacaaaaaa gggcattcta
     2521 ccc
//
LOCUS       DAOSOL010000214         2518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_41302_length_2518_cov_4.694681, whole genome shotgun sequence.
ACCESSION   DAOSOL010000214 DAOSOL010000000
VERSION     DAOSOL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41302_length_2518_cov_4.694681"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK171_05420"
     CDS             complement(<1..246)
                     /locus_tag="PK171_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05660.1"
                     /translation="MDKSIILEARDIVKNFPGVRALDGVQFQLQKGEVHALVGENGAG
                     KSTLMHILGGVYKPDSGDVFVEGKKVKLQNPYHASQEG"
     gene            complement(381..1352)
                     /locus_tag="PK171_05425"
     CDS             complement(381..1352)
                     /locus_tag="PK171_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05661.1"
                     /translation="MKKMMLFIMILVGIFSFMIPAFAEEKLPISEQLYIEVSALGSLD
                     YFYDHKLGMELAGKYLGVKTEYVGPADFDMAAMITAFEQSIAKKPQGILVVGFEPSLD
                     AIVDKAIDAGIPVVTLDADLPGSKRIAFVGTGNYQAGFEGGKKLASLVGEKGKVAIMT
                     RPGQSNLEERIVGYKDALAQYPDIEIVQIADTQSDPTVAAQAAAALLQKYPDLAGIGC
                     VEAAGGAGAATAVNEAGKAGQVKIVSMDRGNDVLEYIKDGIISASVAQQTALMPFYGT
                     QILFNLYNYDIPISSDNAKAKISGTPAVIDTGVVIVDETNYEYFMRD"
     gene            complement(1528..>2518)
                     /locus_tag="PK171_05430"
     CDS             complement(1528..>2518)
                     /locus_tag="PK171_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05662.1"
                     /translation="WKPWTFFDGTPVLVPEKFNIMPESNGDILQYPEGDRSVPASMRM
                     PKDGFYHDSIIRQKPIVDESKLKVEDQVEEYSIMGDEDLACYEKEAKRLYEETDRAVV
                     FGGVPGTNLGDIALVPGPGMKNPQGIRDVEEWYVSLMTRKDFLKEVFSQMTEIGLKNL
                     KLMYEAVGERIQVIVLSGTDFGSQNCPFISPALYRELFKPYHQAMNEWIHQNTQWKVF
                     IHTCGSVFDLLPDLKEAGFDILNPVQVSAAKMDPKELKEHFGSDFTFWGGGVNPQSTL
                     PFGSPEDVRQEVRYLINTFKPNGGFVFANVHNIQAKIPVENLLAFFETLQENR"
BASE COUNT          775 a          563 c          392 g          788 t
ORIGIN      
        1 cccctcctga gatgcatggt aaggattttg taatttaact ttcttaccct cgacgaatac
       61 atctccacta tcaggtttat acactcctcc taagatgtgc attaaagtgc ttttcccagc
      121 tccattttca cccaccagag catggacttc acccttctgt aattgaaatt gaactccatc
      181 aagagctcga actccaggaa agttttttac gatatctcgc gcttctaaaa ttattgattt
      241 atccatgaat tcctccaata acgatgagta tcactaatat ttctacactc cccctcaatt
      301 gaataaataa ccaattatca caagggagcg tgataatgga tacacgctcc cttgtgatta
      361 ctaatttatc tttggttata ttaatctctc ataaaatatt cataattcgt ttcatctaca
      421 ataacaacac cagtatctat gacagctggc gtccctgaga ttttagcttt tgcattatcg
      481 ctggaaattg gaatgtcgta attataaagg ttaaatagaa tttgagtacc ataaaatggc
      541 attaaagcgg tctgctgagc aaccgatgca gagattatcc cgtctttaat gtattctaaa
      601 acatcgtttc ctctatccat agaaacaatc ttaacctgac ccgctttccc agcttcgtta
      661 accgcggtag cagccccagc cccaccagca gcttcaacac aaccaatacc agctaagtct
      721 gggtactttt gaagaagagc tgcagcagct tgagcagcaa ccgttgggtc cgactgagta
      781 tcagcaatct gtactatctc aatatcagga tattgagcta aagcatcttt atatccaaca
      841 attcgctctt caaggttcga ttgacccggt ctggtcataa ttgcaacttt ccccttttcg
      901 cccacgagag aagctagctt tttgcctcct tcaaaacctg cttgataatt gcccgttcca
      961 acaaaggcaa ttctttttga acctggcaaa tcggcatcaa gtgtaacaac cgggatacca
     1021 gcatcaatag ctttatcaac aattgcgtcg agcgaaggct caaaaccaac caccaatatc
     1081 ccctggggtt ttttggcaat ggattgttca aatgcggtga tcatggctgc catatcaaaa
     1141 tcagccggtc caacatactc ggttttaact cccaaatatt ttccagctaa ttccattccc
     1201 aatttatgat cataaaaata atccaagctt cctagtgctg aaacttcaat ataaagttgt
     1261 tcagaaattg gtaatttttc ttcagcaaac gctggaatca taaaactgaa aatacctact
     1321 aatatcatga taaaaagcat catctttttc atgttctttt ccacctcgtt ttattttttt
     1381 ttaaaaactg aacatcttaa attattcgga ttccacctcc caactctatt tttatcatga
     1441 taataaataa ttatatcaat ttattaatta actatttaaa ttataagatg agaatatttc
     1501 ttatttaatt ttaataccat ttaattttta acggttttct tgaagggtct caaaaaaagc
     1561 gagtaaattt tctactggaa tttttgcttg aatattatgg acattagcaa aaacaaaccc
     1621 tccattaggt ttgaaagtat ttatcaaata tctgacttct tgacgaacat cctcaggaga
     1681 accaaaagga agagttgatt gaggattcac tcctcctccc caaaatgtaa agtctgaacc
     1741 aaaatgttct ttaagttctt tcgggtccat tttggctgca gaaacttgca ctggattaag
     1801 aatatcaaaa ccggcttctt ttaaatcagg aagaagatca aatactgagc cacaggtatg
     1861 gataaatact ttccattgag tattttggtg tatccattca ttcattgctt ggtgataagg
     1921 tttaaagagt tctcgataaa gagctggtga aataaatgga caattctggg atccaaaatc
     1981 agtccccgaa aggacgataa cctgaattct ctcaccaaca gcttcataca ttaatttaag
     2041 attctttaaa ccaatttcag tcatctgaga aaatacctct tttaaaaaat ccttacgagt
     2101 catgagggaa acataccatt cttcaacgtc tctaattcct tgaggatttt tcatgcctgg
     2161 tccagggacc aaagcaatat cacctaagtt agttcctggc actccaccaa aaaccaccgc
     2221 acggtcagtt tcttcgtaaa gtctttttgc ctctttttca tagcaagcca ggtcttcatc
     2281 gcccataatc gaatattctt caacttggtc ttccactttt agcttcgatt catcaacaat
     2341 tggcttttgc cgtataatag aatcatgata aaacccgtct tttggcattc tcatagaagc
     2401 cggaaccgaa cgatcgcctt caggatattg aaggatgtca ccattcgatt ccggcataat
     2461 attaaacttt tccggaacca aaaccggtgt cccatcaaaa aaagtccatg gtttccag
//
LOCUS       DAOSOL010000215         2514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_41403_length_2514_cov_3.611224, whole genome shotgun sequence.
ACCESSION   DAOSOL010000215 DAOSOL010000000
VERSION     DAOSOL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41403_length_2514_cov_3.611224"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PK171_05435"
     CDS             complement(<1..1732)
                     /locus_tag="PK171_05435"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOT05663.1"
                     /translation="MKLKWAFFIEIFLVVFMVVSFSIPVFPYNNDNQARTMEGGPHRA
                     INYYAIQSFLNNRRSDSIFNKYDFTNGRDLSGITVIQPGDWKEDILEGEREGKWYQWV
                     VEGGYTADEPERYMSLRHFYDPLGVNQGANYLTDHVDEFLTSIVMGTNPKIDAKWWGA
                     LYSPYSIDKGKEFMITAFSSMNLPEKDKLFAAAWRSLGETMHLIADMTVPSHVRNDSH
                     PGVEWTQFITDKYRADSYEEFVDQQVVEKCLGSPDPVLVEAVNQIPKNEDSFLNVLSL
                     YDCVAGYTNRNYFSLDTIAGIEKALGQGITNSNGMPEYPSPRLEDLETDEDGYYYCKD
                     YLGKLYLARMLYQEKLSLFGKLSLIPKPEIDHQCALSQAKRLIPAAIYANEQLIEWFI
                     PKVEVIIDSFILNEKGTDGREGIIKGRVVHIPSGMYDQPLLFTMPIDGMISIVMNGEI
                     FETQPGSISIQNGIIEGKVQSLPTGPIKNVNLLVDIGGILVSSADLKIYNLQLHPEKL
                     DGLSKKKYGFYVQSDNPPSWVSYIWDFGDGRVEETNSTSITHTYESEGEYQIQVQMRD
                     ANTGKILAKAQ"
     gene            2201..>2514
                     /locus_tag="PK171_05440"
     CDS             2201..>2514
                     /locus_tag="PK171_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05664.1"
                     /translation="MPKKCLLFYILLILFTILASSTLSHALPEFRGVWIDIKSIPNTR
                     DGIETMVKKLASAHFNAILVESFYLGKTIYPSDFLAQQGLTRQMESFEQSNLDPLVVF
                     IEV"
BASE COUNT          731 a          544 c          420 g          819 t
ORIGIN      
        1 cctgagcttt ggcaagaatt ttcccagtat tggcgtctct catttgaacc tggatttggt
       61 attcgccttc tgattcataa gtatgagtaa ttgacgtgct attggtttct tcgactcttc
      121 catcgccaaa atcccaaata tagctcaccc aggaaggagg attatccgat tgcacataaa
      181 agccgtattt ctttttactc aaaccatcta atttctcagg atgtaactga agattataga
      241 ttttcagatc cgctgatgaa acgagaatcc caccaatatc caccaataag tttacatttt
      301 tgattggtcc agttggcaac gactgaacct ttccctctat gattccattt tgaattgaaa
      361 tagatcccgg ttgagtttcg aagatttctc cattcatcac aatgctaatc atcccatcga
      421 tgggcatagt aaataaaaga ggctgatcat acatacctga aggaatatga actactcgac
      481 ctttaatgat accttctctg ccgtctgttc ctttttcatt aaggataaaa gaatcaatta
      541 ttacttcgac tttggggatg aaccattcta tcaattgctc attggcataa atcgctgccg
      601 gtattaaacg cttagcttgg cttaaagcgc attgatggtc aatttcaggt tttggaatta
      661 agcttaattt accaaaaagc gacaactttt cctgataaag cattcgtgct aaatagagtt
      721 tccctaaata gtccttacag tagtaatacc catcttcatc agtctcaaga tcttccaaac
      781 gcggtgatgg gtattccggc ataccgttgc tattggtaat tccttgacct aatgcttttt
      841 caataccggc tatggtatca agtgaaaagt aatttcgatt ggtataacct gcaacgcagt
      901 catacaggct taaaacattt aaaaaactat cttcattttt gggtatttgg ttaactgcct
      961 ctactaacac tggatcggga gaacctaagc atttttcaac aacttgctga tcgacaaatt
     1021 cttcataaga atcagctcga tatttatcgg tgataaattg cgtccactca actccaggat
     1081 gggaatcatt acgaacatgg gaaggaactg tcatatcggc tattaaatgc atagtttcac
     1141 ccaatgaccg ccaagcagct gcgaacaatt tatccttttc tggtaaattc atagacgaaa
     1201 aggccgtaat cataaattcc tttcccttat caatcgaata gggtgaatac aatgctcccc
     1261 accatttcgc atctattttg ggattggttc ccataacaat tgacgttaaa aattcatcga
     1321 catggtcggt aagataattt gctccctggt taacacctaa tgggtcataa aaatggcgga
     1381 gtgacatgta tctttccggt tcgtcggcgg tatagccacc ttctaccacc cattgatacc
     1441 attttccttc tctttcaccc tcaagaatgt cttctttcca atcgccaggt tggattaccg
     1501 tgatcccgga caaatccctt ccgttggtaa aatcatattt attaaagatg gaatcgcttc
     1561 tcctattatt taaaaatgac tgaatggcat aataattaat ggctcgatgg ggtccgccct
     1621 ccatggttcg ggcttggtta tcattattgt agggaaaaac cggaattgaa aaggaaacaa
     1681 ccataaagac tactaaaaat atttctataa aaaatgccca tttcaatttc attatatttc
     1741 cctcctttcc tggctatatt ttaaaaattt ttattgaata atttattttt attgttcctt
     1801 tctattttga aaatacacag gcatgataaa tcaagctctt acaaaagaat aaaaaatgta
     1861 ggggcacaat ctatagcgcc cgattaatgt agcgacatgc catggcatgt cgaatcttgg
     1921 gttttcatcc tcatctggtg ccaccaaagt ggcatgcatg tttaccctga aaataacgta
     1981 acgagtgacg agtaatcagt gatgagttta aatttcaccc tcatcctcac cttctcccat
     2041 caagagagaa ggaactcttt attctttcat tcatttttct ccccatctcc cccttagtct
     2101 tttctcgtcc ccttactcta ttttctccaa ctattgcatt tcagatctgg ctttggaata
     2161 atgaataaaa ttccaattca aactaaagga gaaagctccc atgccaaaaa aatgccttct
     2221 attttatatt ttattaatcc tttttaccat cttggcttct tcaaccctaa gtcatgctct
     2281 ccctgaattt cgtggggtat ggattgatat aaaaagtatt cccaatacgc gggatggaat
     2341 tgaaaccatg gtcaaaaaat tagcttcggc tcatttcaat gccattttag ttgaatcttt
     2401 ttatctggga aaaactattt atccttcaga ttttttggct caacagggtc tcacccgcca
     2461 aatggagtca tttgagcaat ctaaccttga tcctcttgtc gtctttatcg aagt
//
LOCUS       DAOSOL010000216         2510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_41483_length_2510_cov_3.737678, whole genome shotgun sequence.
ACCESSION   DAOSOL010000216 DAOSOL010000000
VERSION     DAOSOL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41483_length_2510_cov_3.737678"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..2478)
                     /locus_tag="PK171_05445"
     CDS             complement(16..2478)
                     /locus_tag="PK171_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT05665.1"
                     /translation="MFERYTERARKVIILAQDEAVRLKHNYIGTEHLLLGLLREREGI
                     AAKILESLDISIEAVRNELENFVDRTEYQGATEVAFTPRAKRVLELALDETRRLSHKY
                     VGTEHILLGVFREGDGVGAQILRRLGLDIETVRMRLNQILNENSQQQDPFAPTSQSKR
                     ESKTPILDEFSRDLTQLAKEGKLDPVIGREREIERVIQILSRRTKNNPVLIGEPGVGK
                     TAIVEGLGQKIIKGEVHEILKNKRVVSLDLAAIVAGTKYRGEFEKRLKKIISEIVQTR
                     EVILFIDEVHTLVGAGAAEGAIDAANILKPALARGELQAIGATTITEYRKYIEKDSAL
                     ERRFQPVYVDEPSVKNSIEILRGIKERYEDHHRVEIVDEALKAAVHLSQRYISDRYLP
                     DKAIDVMDEASSRVRLRATVLPDDLKQMEKEIEETRIKKEMSVKQQDFEEAATLRDLE
                     DQLKRKYRKNKDEWLQQIDTMRPLVTEEDIAAVVANWTGIPVSRLAIEEKKRLVNMDE
                     EIHKRLIGQDEAVKAVSRAIRRSRAGLKDPRRPIGSFIFLGPSGVGKTELGKALAEFL
                     FGKEDSLITLDMSEYMEKHTISRLIGSPPVYIGYDEGGQLTEKVRRHPYSVILFDEIE
                     KASPEIFNILLQILEEGRLTDAQGRQVDFKNTVIIMTSNLGARMISSNVSIGFGNTTD
                     EGCTYEDIKQKVMEEVRRVFVPEFLNRIDELIVFKPLKHEEIEKIVDLMMKETVKRLV
                     EQSITIELTPEARSKIAKEGYDPNFGARPLRRAIQRMVEDPVSDLILKGTFKEGDRIL
                     IGVEEEILRFDKMLPLELSLKD"
BASE COUNT          639 a          583 c          437 g          851 t
ORIGIN      
        1 tccgattttt tcacactaat cctttaacga aagctcgaga ggcaacattt tatcaaagcg
       61 aaggatttct tcttctactc cgatcaaaat ccggtctcct tctttaaatg taccttttaa
      121 aatgagatcc gaaacgggat cctccaccat tcgttggatc gctcttctta aaggtcgagc
      181 accaaagttt ggatcatatc cttctttggc aatttttgaa cgcgcctctg gcgttaattc
      241 aatggttata gattgttcaa caagacgttt gacggtttcc ttcatcatta aatcaacaat
      301 tttttcaatt tcttcatgct ttaagggttt aaatacgatt aattcatcaa tccgattaag
      361 gaattcagga acaaatactc tccttacttc ttccatgact ttttgcttaa tgtcttcata
      421 agtgcaacct tcatcggtcg tatttccaaa accaatggaa acattggatg agatcatccg
      481 agctcctaaa ttggatgtca tgatgatgac ggtattttta aagtctacct gtcttccctg
      541 ggcatcggtc aaacgccctt cttcaagaat ttgaagaaga atgttaaaaa tttccggact
      601 ggccttttca atttcgtcaa ataaaatgac tgagtaagga tgtcgacgaa ctttttccgt
      661 caactgtcca ccttcatcat aaccaatata tacaggagga gaaccaatta atcgagatat
      721 ggtatgcttt tccatgtatt ctgacatgtc aagggtgatg agtgaatcct ctttcccaaa
      781 aagaaactct gccaatgctt tccctaattc agtttttcct actcccgacg gtcccaaaaa
      841 gataaatgaa ccgattggtc gacgggggtc tttaagaccg gctcgagatc ttcgtatcgc
      901 ccgagatact gcttttactg cttcgtcctg accgattaat cgtttatgaa tttcttcatc
      961 catgttgacc agacgtttct tctcttcaat tgctaaccgg gaaaccggta ttccagtcca
     1021 gttggctacc actgctgcta tatcctcttc agttaccaaa ggtcgcatgg tgtcaatctg
     1081 ctgtagccat tcatctttgt ttttccgata ttttctcttt aattgatctt ccagatctct
     1141 cagagttgca gcctcttcga aatcttgttg ttttaccgac atttcctttt ttatacgggt
     1201 ttcttcaatc tctttttcca tttgcttcaa gtcatctggt aaaaccgttg ctcgcaaacg
     1261 aacccggcta cttgcttcat ccattacgtc gatagctttg tcgggaagat aacggtcgct
     1321 aatgtaacgc tgagaaaggt gaacggcggc cttgagagcc tcatcgacta tttcaacccg
     1381 atggtggtct tcatatcgct ccttaattcc tctcaaaatt tcaatactat tttttactga
     1441 cggctcatcg acatacaccg gctgaaaacg tctttctaaa gctgaatctt tttctatgta
     1501 ttttcgatat tcagtgatgg ttgttgctcc aattgcttgc aattccccgc gggctaaagc
     1561 tggttttagt atattggcag catcgatggc tccttcagca gcaccagcac caactaaggt
     1621 atgaacttca tcaataaaca atatgacttc cctggtttgg acgatttcag aaattatttt
     1681 cttaagcctt ttttcgaatt cacctcgata tttggttccg gctactattg cggctaagtc
     1741 gaggctaact actcttttat tttttagaat ctcgtgaacc tcgcctttaa taattttttg
     1801 acctaatcct tcaacaatag cggtttttcc aacgcctggt tcaccaatca aaaccggatt
     1861 attcttggtt cgccggctca agatttgaat aactctttct atttctctct ctcggccaat
     1921 aactggatct aattttcctt ctttggcaag ctgggttagg tctcggctga attcatccaa
     1981 aattggagtt ttactctctc ttttggattg actggtgggt gcaaaaggat cttgttgttg
     2041 ggaattttca tttaatattt gattcaatct cattcggacg gtttcgatat caagcccgag
     2101 tctcctgagt atctgtgcac ctactccatc tccttctcga aaaactccaa gcagaatatg
     2161 ttcggtccca acatatttat gagataatcg acgcgtttca tccaaagcca attccaagac
     2221 tctcttggca cgcggtgtaa aagctacctc agttgcacct tgatattcag tcctgtcaac
     2281 aaaattttct aattcattgc gaactgcttc aatgcttata tcgagacttt caagaatttt
     2341 tgctgctatt ccttctcgtt ctcttaataa acctaaaaga agatgttcag tccctatata
     2401 attgtgcttg aggcgcacgg cttcgtcttg agccaatata attacttttc tggctctttc
     2461 ggtatatcgc tcaaacatcg caaagttcct ccccagagat aactctgaaa
//
LOCUS       DAOSOL010000217         2503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_41645_length_2503_cov_3.544118, whole genome shotgun sequence.
ACCESSION   DAOSOL010000217 DAOSOL010000000
VERSION     DAOSOL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41645_length_2503_cov_3.544118"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PK171_05450"
     CDS             <1..523
                     /locus_tag="PK171_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOT05666.1"
                     /translation="TYNDLLEAEPSKATTLSRFELTYLLSKMNVAEIMEKNVITIKPD
                     IPIEEAALIMKKNKVGGLPVVEETRVVGMITESDIFDVLVETLGVELGGTRITLELPE
                     KPGELHRVTGIVSQFMVNILSLATFYINERPDLRYVVIRVATRDYEPIIEAFKADGIT
                     VKHVWVSPVDEEV"
     gene            526..1086
                     /locus_tag="PK171_05455"
     CDS             526..1086
                     /locus_tag="PK171_05455"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT05667.1"
                     /translation="MFFPYDEKKLPKLVIPEIKSSEILCETRLFQLKRNTYVDDLERI
                     FITHPGAVTIIATTPENQIILIRQFRAPAGQWLWEIPAGTLEPDEDPLDCAIRELEEE
                     TGFTSKHWKYLFPVFLAPGYSTELIHFFWASRAYPVEKARKGDDDEEIYYLKVTPEKA
                     QNMMAKGAIRDAKTMIALQYWLGGLT"
     gene            1083..1994
                     /locus_tag="PK171_05460"
     CDS             1083..1994
                     /locus_tag="PK171_05460"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOT05668.1"
                     /translation="MRIFIALLDGLGMGELPDAAEYGDEGSHTLANLSRKVGGLSCPT
                     LERLGLGKIEPILGIVAVNKAEGWYGKMIEKSVGKDTTTGHWEMMGIITERPFPVYPH
                     GFPKEIINEIERVTGFQVLGNKPASGTTIIEEYGAEHIRTGFPILYTSADSVLQIAAH
                     EEIISIKDLYRLCEMVRGIMKGDHAVARIIARPFVGQPGNFQRTSRRKDFSLSPPYPT
                     VLDVLLEKGIQVAGVGKIGDVFTGRGISENFKTTSNSDTFRVFFELLDDERFGLIWAN
                     FNDFDTLYGHRNDTIVFRKLLNPGMLN"
BASE COUNT          711 a          460 c          570 g          762 t
ORIGIN      
        1 tacttataac gacttgttag aagccgaacc atctaaagcg accaccttga gtcgttttga
       61 gttgacttat cttcttagta aaatgaatgt tgctgaaata atggaaaaaa atgtaatcac
      121 cataaaaccc gatattccaa tcgaagaagc cgctttaatc atgaagaaga acaaagtcgg
      181 tggccttccg gtagtcgagg aaactcgtgt ggtgggaatg ataactgaat cggatatttt
      241 tgatgtctta gttgaaactc tgggagtaga gttaggtgga acccgaataa ctcttgaact
      301 tcccgaaaaa ccaggtgaat tgcatcgggt tacaggcatt gtttctcaat ttatggttaa
      361 tatattgagt ttggccactt tttatataaa tgaaagacca gacttacgtt atgttgtaat
      421 acgggttgca actcgcgatt acgaaccaat tattgaagct tttaaagccg atggtattac
      481 cgtgaaacat gtctgggttt ccccagtaga tgaagaggtg taagggtgtt ttttccttat
      541 gatgaaaaaa aactaccaaa acttgttatt cctgaaataa agagcagcga aatactctgt
      601 gaaactcgtt tatttcagct aaaaagaaat acctatgttg atgatttaga gcggattttc
      661 attactcatc caggagcagt gacaattatt gcaactactc cggaaaatca aattatttta
      721 atacggcaat tccgagcacc ggctgggcag tggctttggg agattccagc tggaaccttg
      781 gaaccagatg aggatccatt ggattgtgca attcgagaat tggaggagga aactggtttt
      841 accagtaaac attggaaata cctttttcca gtatttctcg caccaggata tagtacggaa
      901 ttaattcatt ttttctgggc ttctcgggcg tacccagttg aaaaagctcg aaagggggat
      961 gatgacgagg aaatttatta tttaaaagta accccagaaa aagctcagaa tatgatggcc
     1021 aaaggagcaa tacgagatgc caagactatg attgccttgc aatattggct gggaggatta
     1081 acttgagaat atttattgct cttcttgacg gtctgggaat gggtgaactt cctgatgctg
     1141 ctgaatatgg agatgagggt agccatactt tagccaatct ttctcgaaag gttggtggtt
     1201 tgtcttgccc aactttggaa agattaggat taggaaaaat cgaaccaata ctgggaattg
     1261 ttgcagtaaa taaagccgag ggttggtatg gaaagatgat agaaaaatca gtagggaaag
     1321 atacgactac cggccattgg gagatgatgg gaatcattac cgagcggcct tttccggttt
     1381 atccgcatgg tttccccaaa gagattatca atgagatcga aagagttacc ggttttcagg
     1441 ttttggggaa caaaccggca tccggaacca ccattattga ggaatacggc gcagaacata
     1501 tccgtaccgg ttttccaatt ttatatactt cggccgatag tgtgttgcaa atcgctgctc
     1561 atgaagaaat catctcgata aaagacctgt atcgtctttg tgaaatggtt cggggaatta
     1621 tgaagggcga tcatgctgtt gcccgtatta tcgctcgtcc ctttgttggt caaccaggta
     1681 actttcaacg gacctcaagg cggaaagatt tttcactgtc ccctccttac cctacagtat
     1741 tagatgttct tttagagaag ggaattcaag ttgccggagt tggaaaaatt ggtgatgttt
     1801 ttactgggcg aggaatcagt gaaaatttta agaccaccag taactccgat acctttcggg
     1861 tattttttga attactcgat gatgagcggt ttggtttgat ttgggccaac ttcaatgatt
     1921 ttgataccct ctatggtcat cgtaatgaca ccattgtttt cagaaagctc ttgaatcctg
     1981 ggatgctcaa ttaaaagaat ttctccctcg attacgtttt aatgacctat taatcattac
     2041 cagcgaccat gggtgtgatc caaccacgcc tagcactgat cattcccgcg agcatgcact
     2101 tcttatctgt gcctctcccc aattattaat gggggattct ttgggtatcc gcaattcttt
     2161 tgctgatatt ggggcgacag ttgcccaatt gtttggaatt ccttggtcgg gtccgggaac
     2221 cagtttttcc ttcataattg gtgattaatc gggaaggaag aaggtttaga tccgatgact
     2281 ttctatttta agagagtaaa tcattttccg ccggtcctac atcctatcca ggagtctaat
     2341 taataatgaa tattgttatc agccatgaag gtactgattt tgatgcctta gcctcgatgt
     2401 ttgcagtttc caaactttat ccgggaaccc gcattattat cggtggtagt gtgaatcgaa
     2461 atgttcggcg ctttcttact ctttatggga ctttttttaa tta
//
LOCUS       DAOSOL010000218         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_42053_length_2486_cov_3.582476, whole genome shotgun sequence.
ACCESSION   DAOSOL010000218 DAOSOL010000000
VERSION     DAOSOL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42053_length_2486_cov_3.582476"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="PK171_05465"
     CDS             complement(<1..95)
                     /locus_tag="PK171_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL30 family ribosomal protein"
                     /protein_id="HOT05669.1"
                     /translation="MSKKLLKITLKKSVIGRQKSHRRTVRSLGLK"
     gene            complement(111..617)
                     /gene="rpsE"
                     /locus_tag="PK171_05470"
     CDS             complement(111..617)
                     /gene="rpsE"
                     /locus_tag="PK171_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003194373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOT05670.1"
                     /translation="MQKVKPEGLELQERLISVSRVSKVVKGGKRFGFRAIVAVGNGDG
                     VVGLGIGKAKEVPDAVRKAVERAKKSLFSFPRRKMTITHEVLGKYGAGSVVMKPASPG
                     TGVIAGGPVRAIMEVAGIRDILTKSLGTNNPVNVAHATLQGLMSLKDPREVAHLREKN
                     SQDFFERV"
     gene            complement(635..994)
                     /gene="rplR"
                     /locus_tag="PK171_05475"
     CDS             complement(635..994)
                     /gene="rplR"
                     /locus_tag="PK171_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOT05671.1"
                     /translation="MPLIDKKQKRVYRHNRVRSKVKGTAERPRLCIYRSLSHIYGQII
                     DDDKGETLAAFSTLSAPMKTISGTKTEKAREVGKGIAQIALQKGIKQVVFDRGGSKYH
                     GRVRALAEGAREGGLEF"
     gene            complement(1007..1543)
                     /gene="rplF"
                     /locus_tag="PK171_05480"
     CDS             complement(1007..1543)
                     /gene="rplF"
                     /locus_tag="PK171_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOT05672.1"
                     /translation="MSRIGKKAIVVPSGVNIEIDGLSVRVKGPKGLLERQFHPRMKIV
                     KEVNEIKVVPQGETKLDKSLHGLTRTLIANMVHGVSDGFEKSLELVGVGYKAQKKGNT
                     LVMNLGFSHPVEIVPPSGISFDVEGQIIKVKGANKEQVGQVAADIRKIRKPEPYKGTG
                     IKYINEFVRRKQGKTTAK"
     gene            complement(1560..1961)
                     /gene="rpsH"
                     /locus_tag="PK171_05485"
     CDS             complement(1560..1961)
                     /gene="rpsH"
                     /locus_tag="PK171_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010908572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOT05673.1"
                     /translation="MAHTDPIADMLTRIRNSLKARENSVVIPYSKLKGELARILKEEG
                     YIQNYKIVARENKSSDMIIDLKYGMKKERIINGLKRISKPGLRIYAGKDEIPVLLGGM
                     GTVIVSTSQGIMTGKTAKKSGVGGEVICYIW"
     gene            complement(1977..2162)
                     /locus_tag="PK171_05490"
     CDS             complement(1977..2162)
                     /locus_tag="PK171_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOT05674.1"
                     /translation="MARKAKIVRNQQQLKYKNRYRNRCALCGRSRGYLRDFGMCRICF
                     RSLANKGEIPGVTKSSW"
     gene            complement(2177..>2486)
                     /locus_tag="PK171_05495"
     CDS             complement(2177..>2486)
                     /locus_tag="PK171_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOT05675.1"
                     /translation="KLRKGNPIGCKVTLRVQRMFEFLDRFLNISIARIRDFRGFPDGC
                     FDGRGSCTIGIREQLIFPEINYDKVERVRGMNITFVTTAKTDEEAKALLEVLGLPFRK
                     "
BASE COUNT          627 a          634 c          399 g          826 t
ORIGIN      
        1 tttttaagtc ctaatgatcg cacggttcgt cggtgtgatt tttgcctacc gattacactc
       61 ttttttaagg taattttcaa taattttttg ctcataaacc tatcactccg ctatactctt
      121 tcaaaaaaat cctgggaatt tttttctctc aaatgtgcta cttcccgagg atctttgagg
      181 ctcattaatc cctgcaaagt agcgtgggca acgttaacag gattgttggt tcctaatgac
      241 ttggtcaata tatcacgaat accggcaact tccataatgg ctcgtactgg acctccggca
      301 atcacaccgg ttccgggcga agctggtttc atcaccactg aacccgctcc atatttcccc
      361 agcacttcat gggtaattgt catctttcgt cttgggaaag aaaaaagtga ttttttggcc
      421 ctctcaaccg ctttgcggac agcatctggt acttctttag cttttccaat tcctaagcca
      481 actactccgt caccatttcc cactgccaca attgccctga aaccaaatcg ctttcctccc
      541 tttacgacct tactcactcg actaactgat attaatcttt cttgaagctc aagcccttct
      601 ggcttcactt tctgcattgt ccaacctcca aggattaaaa ttcaagaccg ccttctcgag
      661 caccctcagc taaagctcta acccgaccgt gatacttact tccacctcgg tcaaacacca
      721 cttgcttaat tcccttttga agagcaattt gagcaatccc cttacccact tcccgagctt
      781 tctcggtttt ggttccagag atagtcttca tcggtgctga aagggttgaa aaagccgcca
      841 aggtttctcc cttatcatca tcaataattt gaccgtaaat atgacttaaa ctccggtaaa
      901 tacagagtcg gggacgttcg gcggttcctt tcacttttga acgtactcgg ttatgtcggt
      961 aaactctttt ttgtttttta tctatcaaag gcatgttcgg tcctccttac ttcgccgtgg
     1021 tcttcccctg ttttcttcgg acaaattcat taatatactt aattcctgtt cctttatagg
     1081 gttcaggttt tctaattttt cgaatgtcag ctgctacctg cccaacttgt tccttattag
     1141 cgcctttcac tttgattatt tgtccttcaa catcaaacga aattccgctg ggaggaacaa
     1201 tctccaccgg atgactgaat ccgaggttca taaccaaagt gtttcctttt ttctgagctt
     1261 tatagccaac tccgaccagt tccaatgatt tttcaaaacc atcactaacc ccatgaacca
     1321 tgttagcaat cagagttcga gttagtccgt gaagagactt atcaagcttc gtctctcctt
     1381 gtggaaccac tttaatctca ttcacctctt taactatttt catgcgtgga tgaaattgtc
     1441 tctctaataa tcccttggga cccttcaccc gaacggatag accgtcgatc tcaatattga
     1501 ctcccgaagg aacaacgatc gctttttttc ctatgcgtga catatcatgg atcccctttc
     1561 taccatatat aacagatgac ctctccgcca actcctgatt tttttgcggt ttttccagtc
     1621 ataattccct gtgaggtcga tacgattacc gttcccatcc cacccaagag aacgggtatt
     1681 tcatcttttc ctgcataaat ccgtaaacct ggtttactga ttctttttaa accattgata
     1741 attcgctctt ttttcattcc atatttcaga tcaattatca tatcacttga cttattctca
     1801 cgagctacta ttttatagtt ttgtatatat ccttcttctt ttaaaatgcg ggctaattct
     1861 ccttttagct tcgaataagg tatcaccaca gaattttctc gcgcctttag agaattccgt
     1921 atacgggtaa gcatatcagc aataggatca gtatgtgcca ttttatttat tccttcctac
     1981 caactcgatt tggtaacacc gggtatttct cccttattag ccagggaccg gaaacagatt
     2041 cgacacatgc caaaatcccg taaatatcct cgggaccggc cacacaaggc gcaacgattt
     2101 cggtatcgat ttttatattt taattgttgt tggtttcgaa caattttggc ctttcgagcc
     2161 attcagtttt cctcctttac tttcgaaaag gtaatcccaa aacttcaaga agtgctttgg
     2221 cttcttcatc ggttttggca gttgtaacaa aagtaatatt catacctctc actctttcaa
     2281 ctttatcata atttatttcg ggaaaaatga gttgttctcg aatcccaata gtacaactcc
     2341 ccctaccgtc aaaacaccca tcaggaaatc ctcgaaagtc tctaattctt gcaattgaga
     2401 tattgaggaa acggtccaaa aactcaaaca tccgttgaac ccgaagggta accttacatc
     2461 caattggatt tccttttcgc aattta
//
LOCUS       DAOSOL010000219         2481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_42152_length_2481_cov_2.920445, whole genome shotgun sequence.
ACCESSION   DAOSOL010000219 DAOSOL010000000
VERSION     DAOSOL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42152_length_2481_cov_2.920445"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..>2481)
                     /locus_tag="PK171_05500"
     CDS             complement(117..>2481)
                     /locus_tag="PK171_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT05676.1"
                     /translation="WFAAMELPTNRRIIIGGDFDFPPFEFINENGQPDGFNVDLTKAI
                     AKEMDLDIEIHLGPWAKIREDLFNGKIDAIQGMLYSSERDLLFDFTPPHTNISYVSVV
                     RTGEGNPPSSLDDLRDKQIIVQQGDIMHDFLQKNGMEKQALLVESQKDALQILAEGKY
                     DCALVSLRTAYYWIEKQGWDNLTVGQEPLVTAEYCFAVHQNQRALLTQLSEGLNVLSK
                     TGEYRQIFEKWMGIYDLKTLKLITILRYIAIVIIPLLVLLFIFFLWSWSLRKQVVKRT
                     RELRESERQKELILNSTNELITYYDTNLSIIWTNRLSNESIGKTPEEVVGKRCYELWH
                     QRNEPCAECPVLTALHDKAPREANNQTPDGRYWHMRGYPIFDKKGQVVALAEFTQEIT
                     ERKKMEEEHEKLQAQLLQAQKMESVGRLAGGIAHDFNNMLQAILGHAELALNQVDPFH
                     LIHADLIEIHHAAERSANLTRQLLAFASKQTIAPKLLDLNQIVEGTLKMLGRLIGEEI
                     EPVWIPGKDLWLVYIDPSQIDQISANLCVNARDAISGTGKITIETGNAIFDDNYCEQH
                     YGYIPGEYVLLSVSDNGCGMDQKTIAHVFEPFFTTKGFGKGTGLGMSTVYGIVKQNNG
                     FINVYSEPGKGTTIKIYLPRFKKESEPIQVGTPKVGLTRGRETILLVEDDQAILKMTE
                     KMLKQLGYQILTAQTPKEAINIAQKYTNNIHLLITDVVMPDMSGPELTERLLTIFPNI
                     KVLFMSGYTANVIAHQGVLDEGVHFIQKPFSIKDLSTKVRSILENID"
BASE COUNT          711 a          478 c          485 g          807 t
ORIGIN      
        1 attacctata attgtgttaa tcgggtaatt ccttaggctt cttaaaaaca ttatgtgaga
       61 aagaaagtaa ttaatagtac cttgaaagat atctggaaat aaagtcaatt tatggtttaa
      121 tcgatatttt ctaaaatact acgaacctta gtactgaggt cttttattga aaagggtttt
      181 tgaatgaaat ggactccttc atccagaact ccttgatgag cgatgacatt agcagtataa
      241 ccagacataa aaagaacttt tatattagga aatattgtta ggagtctttc ggtaagctcg
      301 gggccactca tgtcaggcat aacaacgtca gtaataagta gatgtatatt attagtgtat
      361 ttttgagcta tattaatagc ttctttggga gtttgagcag ttaaaatttg ataccctaat
      421 tgttttaaca ttttttcagt cattttaagg atggcttggt catcctctac taacaaaatt
      481 gtttctctcc ctctagttaa tcccaccttt ggtgttccta cttgaattgg ttccgattcc
      541 tttttaaatc gaggcaaata aatcttgatg gtggttcctt ttcctggttc actatagaca
      601 ttgatgaagc cgttattttg tttaacaata ccgtaaacgg ttgacattcc taagccggtg
      661 cctttcccaa atcctttggt ggtgaaaaaa ggctcgaata catgagcgat agttttttga
      721 tccattccac acccattatc gctcactgat aaaagtacat attcaccagg tatataacca
      781 taatgttgtt cacaatagtt gtcatcaaaa atagcgttgc cggtttcgat ggttattttt
      841 ccagttcccg aaatggcgtc acgagcgtta acacagaggt tggcactgat ttggtcgatt
      901 tgtgaaggat caatgtaaac taaccataaa tccttacctg ggatccagac aggctcaatt
      961 tcttcaccaa ttaaccgacc cagcattttt aaggtgccct caacgatttg gttcagatcg
     1021 agtaacttcg gagcaatggt ttgtttgctg gcgaaagcta ataactgccg agttaaattg
     1081 gcggaacgtt cagccgcatg gtgaatttca atgaggtcag catgtataag atgaaaggga
     1141 tctacctgat ttaaagctaa ttcggcgtgg ccgagaatgg cttgcagcat attgttgaaa
     1201 tcatgagcta tacctcccgc cagccttcca actgattcca tcttttgggc ttgcaggagt
     1261 tgagcttgga gcttttcgtg ttcttcttcc atttttttcc tttcggttat ctcctgagta
     1321 aactcagcaa gcgctacgac ttggcctttt ttatcaaata ttggataacc gcgcatgtgc
     1381 cagtagcgac catcaggagt ttgattattt gcttcgcgag gtgctttatc atggagagcc
     1441 gttagaactg ggcattcggc acaaggttca tttctttgat gccacaattc atagcaacgc
     1501 tttccaacga cttcctcagg agtttttcca attgattcgt tggaaaggcg attcgtccaa
     1561 ataatactta agttggtatc ataataagtg attaactcat tagttgaatt taaaatgagt
     1621 tccttttgtc gttcactttc acgtaattcc ctggttcgtt tgacaacctg tttgcgaagc
     1681 gaccatgacc agaggaaaaa tataaagagc agaacaagaa ggggaataat aactatggca
     1741 atatatcgta agatagtgat cagttttaaa gtttttaggt cgtaaatgcc catccatttt
     1801 tcaaaaattt gacgatattc tccggtttta gaaagaacat ttaatccttc gcttaattga
     1861 gtcaaaagtg ccctctgatt ttgatgcaca gcaaaacaat attcggcggt tactaatggt
     1921 tcttgaccaa cagttaggtt atcccaaccc tgcttttcga tccagtaata ggcagttctc
     1981 agtgaaacca gtgcacaatc gtactttccc tcggctaata tctgaagggc atccttttgt
     2041 gattcaacta aaagggcttg tttttccata ccattttttt ggagaaaatc gtgcataatg
     2101 tcaccctgtt gaacgataat ctgtttatct ctcaaatcat ctaaggatga tggtggattt
     2161 ccttctcccg ttcttaccac gcttacataa ctgatatttg tatgaggagg agtaaagtca
     2221 aaaagtaagt ctctttctga agaatagagc ataccctgga ttgcatcaat ttttccatta
     2281 aataagtctt cgcgaatttt agcccaggga ccaaggtgga tctcgatatc gaggtccatt
     2341 tctttagcaa ttgctttggt taaatcaacg ttaaacccat cgggttgacc attttcatta
     2401 ataaactcaa aaggaggaaa atcaaaatct ccaccaatga tgatacgacg attggttggg
     2461 agttccatgg cagcaaacca t
//
LOCUS       DAOSOL010000220         2474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_42329_length_2474_cov_4.310045, whole genome shotgun sequence.
ACCESSION   DAOSOL010000220 DAOSOL010000000
VERSION     DAOSOL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42329_length_2474_cov_4.310045"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PK171_05505"
     CDS             complement(<1..1386)
                     /locus_tag="PK171_05505"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOT05677.1"
                     /translation="MIQITSDLVKKIAEERNEILQIIPLVWEYQPYNAKLIPVMGPEE
                     KYLGVWSLEKAPIFKDPWIENQKFYNNFSFTIYPYSFENYQLLSQEILSLKPETCGVK
                     LSFGTGDRLGLVSSAHLSVLKKYEVFPIVAQQSPRELKKTGRTFQSVLLDAAWGAFAS
                     GFRGRFGADADHIKDEYYLQQAIHSGYSFYTLDVSDYIEKEFMFQSEWDLNQTYQKMN
                     PDQLDLLKRYLGRSYSLGGGFQVSFNEENLLRIVLSLYPVLGFIEQMNSILDERLTNY
                     DLEISLDEGEVLTSYETHFFLSEELHRRGVDFQSLALKFPGSFEKGIDYRGDLNQFRE
                     NLRGQVLIAENIGGYRLSLHSGSDKMTIYPTFFEETHGLFHIKTSGTSWLKALETVSV
                     FNPELFRKIYTLSWENYSENSRDYQVSFKINEIPADLEYLPDKKLQDFLKNDSVRQLL
                     HISYGLILKEYR"
     gene            complement(1416..2444)
                     /locus_tag="PK171_05510"
     CDS             complement(1416..2444)
                     /locus_tag="PK171_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT05678.1"
                     /translation="MKSKMKAFVCRGPKQFSLEEVEIGEPEIGEVKLKVLYAGICGSD
                     LSIVHGRNPFASYPLIPGHEFVGEVVESKDASFRAGQLAAVMPVVGCGECESCHLGNV
                     NRCRKVKLYGVHINGGFAEYVLVKAKNLIPFEEPIQPNQAVFSEPLAVGVHANRINGT
                     SQKDSIAIIGGGIIGSVILKVAKVLGAKSITVIDVIAERLERSLLLGAQDVINSSQHN
                     LRELIKNHHYAGKYDSVFDVVGNDETLDIALDLLKPAGKLVLVAVPSGEKRNIDVLKI
                     FSQEKVLTASRTYSREDFIRALELITTHQVNPLDFITHEYPFNSIDFALDRAENSKNE
                     ELKVIVKM"
BASE COUNT          788 a          530 c          395 g          761 t
ORIGIN      
        1 tcgatactct ttcagaatca aaccatagga aatatgaagg agttgtcgaa ctgagtcatt
       61 ctttaaaaaa tcctggagtt ttttatccgg tagatattct aaatcagcag gaatttcatt
      121 aattttgaat gaaacctgat agtctcggct attttcagaa taattctccc aactcaaagt
      181 gtaaattttt cgaaataatt ccggattaaa aacagaaact gtttccaaag ccttaagcca
      241 tgacgttccg gaagttttaa tatgaaaaag cccgtgagtt tcttcaaaaa aggtgggata
      301 aatcgtcatc ttgtcgcttc cagaatgaag gctcaaacga taaccaccaa tattttcggc
      361 aatcaaaacc tgtccccgga gattttcccg aaattgattt aaatctcctc gataatcaat
      421 gcctttttca aaagatccag gaaatttcaa tgccaaactt tgaaaatcaa cacctcttcg
      481 atgaagctct tccgacaaga aaaaatgtgt ttcgtacgaa gtgagaactt caccctcatc
      541 taaagatatt tcgaggtcat aattggttaa tcgctcatca agaatactat tcatttgttc
      601 aataaaaccc agaactggat ataaggaaag aacgattcgg agtaaatttt cttcattaaa
      661 actaacttga aaaccgccac ccagagaata acttcgacct aaatatcttt ttaataaatc
      721 cagttgatcg ggattcattt tttgataggt ttgatttaaa tcccattccg attggaacat
      781 gaattccttt tcaatataat cgctcacatc cagggtataa aaactatatc cactatgaat
      841 tgcctgctgc aaataatatt cgtctttgat atggtcggca tcggcaccaa acctcccccg
      901 aaatccactt gcaaaagctc cccaagcagc atcgagtaac accgattgaa aggttcggcc
      961 agtttttttc aattcccttg gagactgctg agcaacaatc ggaaaaactt catatttttt
     1021 taaaacactt aaatgagctg aggataccaa ccccaaacga tctccagtac caaaagaaag
     1081 cttaaccccg catgtttccg gtttgagcga aaggatttct tgcgacaaaa gttgataatt
     1141 ctcgaaacta taaggataga tggtaaagga aaaattatta tagaattttt gattttcgat
     1201 ccagggatcc ttgaatattg gagccttttc caagctccac acacctaagt atttctcttc
     1261 aggacccatc accggtatca attttgcatt ataaggttga tattcccaaa ccaaaggaat
     1321 aatttgtaaa atttcatttc tctcttcagc gatctttttt accagatcag aagtaatttg
     1381 gatcaaagtt taatcctcct cttattaacc atttatcaca ttttcactat gacttttaac
     1441 tcttcatttt ttgagttttc agcccgatct aaagcaaaat caattgaatt gaaaggatat
     1501 tcatgagtaa taaagtctaa ggggttcact tgatgggttg tgatcagttc caacgctcgt
     1561 ataaaatctt ctcgggaata ggtacgagat gcagtaagca ctttttcttg agaaaaaatc
     1621 tttaatacat caatatttcg cttctctcct gaaggaactg ccaccaatac caatttaccg
     1681 gctggtttta ataaatccaa agcaatatcg agagtctcat cgtttccgac aacatcaaaa
     1741 acactatcat atttcccagc gtagtgatga ttttttatca gttcccttaa attatgttga
     1801 gagctgttga ttacatcttg agcgcccaat agaagtgacc gttctaatct ttcagcaatc
     1861 acatcaataa ccgttattga tttggctccc aagaccttgg ctacttttaa aatgaccgat
     1921 ccgattattc ctccaccgat gatagcaata gaatcttttt gacttgttcc attgattcga
     1981 ttggcgtgaa ccccaactgc caatggttca gaaaataccg cttgatttgg ttgaatcggt
     2041 tcttcaaaag gaattaaatt tttggctttg accaagacgt attcggcaaa tcctccattt
     2101 atatgaacac catataattt aacttttcga caacggttga cattgcctaa atgacaggat
     2161 tcacattccc cgcatccaac taccggcatg acagctgcca gttgaccagc tcggaaagaa
     2221 gcatcttttg attcaacaac ctctccgaca aactcatgac caggaatgag tggataactg
     2281 gcaaaaggat tccgcccatg aacaatggat aaatccgaac cacaaatgcc agcatataaa
     2341 actttcaact tgacttcccc aatttctggc tcacctatct ctacctcttc taaagaaaat
     2401 tgttttggtc ctcgacagac gaaagctttc attttacttt tcatgacaat attgcctcca
     2461 tggcttattt attt
//
LOCUS       DAOSOL010000221         2473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_42350_length_2473_cov_4.117039, whole genome shotgun sequence.
ACCESSION   DAOSOL010000221 DAOSOL010000000
VERSION     DAOSOL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42350_length_2473_cov_4.117039"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1990
                     /locus_tag="PK171_05515"
     CDS             149..1990
                     /locus_tag="PK171_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT05679.1"
                     /translation="MKKNFYLLIIIFCLITTFTTNAQIKDPNTYVLLTIGEPDSLDPG
                     YAYDSHSGEVLTFVYNNLIAYEGENIFKFIPILATAVPSKENGLITNDGKTYIFPIRK
                     GVFFHNGNELNPEDVEYTFERNILFDSVGGPYWMIIGALFDYQTLNEMVASKIGIPLT
                     DLVNEDGSLKNQEYADRLIKFYQEEIDPAIEVDNDKVIFHLAKPFAPFLNLLSKYAYW
                     SSIMDKQTCIEWGCWDGKADEWWKYYNRKKESSPLFDKTNGTGPFILAEWDRTQQKVV
                     LKKNQQYWGEKAKLEQVIIWGVDEWNTRRAMFEAGDADQVYIPTQYIQQLENLKGSQF
                     YQKERITVTNLQFNWTINKTSPFLGSAQLDGNGIPPDFFTDENCRRAFCYAFDYEAMI
                     NDVLQGYGKRIASVLPSDLLGYNDQLPRYEYDLIKAKEEFQKAWGGKIWDKGFQVTLA
                     YDTGNEVFQTACEILKENIESLNPRFKIQIQGIQWPSFLDSFKLGQLPAIVISWIADY
                     PDPHNLIFSHYHSNGAIGGAQGEGFAEFAKNNLNHQIDAALIETNPESRQKLYENIQI
                     DAYKHALGIPLFQPIEIHLMKNWVKDWVFNPIQCGEANFDIIYKKEQ"
     gene            complement(2072..>2473)
                     /locus_tag="PK171_05520"
     CDS             complement(2072..>2473)
                     /locus_tag="PK171_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT05680.1"
                     /translation="KSYSYGNCVDLQWTDPASRYVETLIHLGIIDPEMKEENYYFYPD
                     KPITRKEAAFWLYCLLDFEHLKKDRILPSDISHLDRPYQTAIKITYQAGIFDGILSDD
                     TFLPDQTLTGKEALHILEQSQQAGKRIHPWD"
BASE COUNT          814 a          406 c          496 g          757 t
ORIGIN      
        1 tgtcctaaaa taagataatt tgtatgagta tattaatcat tttaagaaaa ataccgaaat
       61 atatggtatg gaaaataaat tgcaaattct aaatgatatc aattttttaa atagtgggtc
      121 ataattaagt acttctggag ggggaaaaat gaaaaaaaat ttttacctat taatcataat
      181 tttttgcctc attactacct ttaccaccaa tgcccagata aaggatccga atacctatgt
      241 tcttttaacc attggggaac ctgacagctt ggatcctgga tatgcttacg attcacatag
      301 tggagaagta ttaaccttcg tttataacaa cttgattgcc tatgagggag agaatatttt
      361 taaatttata cccattcttg ccactgctgt tccttccaag gaaaatggat tgataaccaa
      421 cgacggaaaa acctatattt tcccgattag aaaaggcgtt ttcttccata atggaaatga
      481 attaaaccca gaagatgtgg aatatacttt tgaaaggaac attctttttg attcggtcgg
      541 aggcccttat tggatgatta tcggggcttt atttgattac caaaccttga atgaaatggt
      601 tgcgagcaaa attggaatac cattaactga tttagtcaat gaagacgggt ctttgaaaaa
      661 ccaagaatat gctgaccgtt taattaaatt ctatcaggaa gaaattgatc ctgccattga
      721 ggttgataac gataaggtca tttttcattt agcaaaacca tttgctcctt ttttaaattt
      781 gctgtcgaag tatgcgtatt ggagtagtat catggataaa caaacctgca tcgaatgggg
      841 atgctgggat ggaaaagcag atgagtggtg gaagtattac aatcggaaaa aagaatcctc
      901 gcctttattt gataaaacca acgggactgg accgttcatt ttagctgaat gggatcgaac
      961 tcaacagaaa gtagtgttga aaaaaaatca gcagtactgg ggtgaaaaag caaaacttga
     1021 acaagttatc atttggggag tcgacgaatg gaatactcgc cgggcaatgt tcgaagccgg
     1081 agatgccgat caggtttata ttcctaccca atatatccag caattggaga atttaaaagg
     1141 aagtcagttt tatcagaagg agcggattac cgtaaccaat ttacaattca attggaccat
     1201 caacaaaacc agtccctttt tagggagcgc ccaattggat gggaatggaa tacctcctga
     1261 ctttttcact gatgaaaatt gccgacgggc tttctgctat gcttttgatt atgaggccat
     1321 gattaatgac gttctgcaag gttatgggaa aagaattgca tcagttcttc cttctgattt
     1381 attgggatac aatgaccagc ttcctcggta tgaatatgat ttgataaaag ccaaagaaga
     1441 atttcaaaaa gcttggggtg gcaagatttg ggataagggt tttcaagtaa ccttggctta
     1501 tgataccgga aatgaagtat tccagactgc ctgtgaaata ctaaaggaaa acatcgaatc
     1561 tttaaatcct cgatttaaaa tacaaatcca gggaatacaa tggccaagtt ttctggattc
     1621 tttcaaatta ggtcagttac ctgcaattgt aattagctgg attgctgatt atcccgaccc
     1681 ccataactta atctttagtc actatcacag taatggagca attggaggag ctcaaggcga
     1741 gggttttgct gaatttgcta agaataattt gaatcaccag attgatgctg cacttattga
     1801 aaccaatcca gaatcccggc aaaaattgta tgaaaatatt caaatcgatg cttataagca
     1861 tgccctggga atacccttat ttcaacccat agaaatccat ttgatgaaaa actgggtcaa
     1921 agattgggtg tttaatccaa ttcaatgcgg tgaagctaat tttgatatca tctataaaaa
     1981 agagcaataa acatttgatg aaagaagcat caaatgttta ttgctcttta aaaatatgat
     2041 tgttggtgga tatcaacttc ttgattaagt tttaatccca agggtgtatt cgttttcctg
     2101 cttgctggga ctgttccaaa atatgaagag cttctttacc ggtgagggtt tgatcgggta
     2161 aaaaggtatc atcagataaa attccatcaa aaatgccagc ttggtatgtt atctttatgg
     2221 cagtttggta aggacgatct aaatgactta tatctgaggg aaggattcga tcttttttaa
     2281 ggtgttcaaa gtcaagtaaa caatataacc aaaatgcagc ttcttttctg gtgatgggtt
     2341 tatcaggata aaagtaataa ttttcttcct tcatttccgg atcgatgatt cccaaatgaa
     2401 tgagagtttc tacatatcta ctggcaggat cagtccactg aagatcgaca caattcccat
     2461 aagaataaga ttt
//
LOCUS       DAOSOL010000222         2463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_42576_length_2463_cov_3.041113, whole genome shotgun sequence.
ACCESSION   DAOSOL010000222 DAOSOL010000000
VERSION     DAOSOL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42576_length_2463_cov_3.041113"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PK171_05525"
     CDS             complement(<1..578)
                     /locus_tag="PK171_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOT05681.1"
                     /translation="MQRALLAGVLIGSLCAVIGIYVVLKGMSFMGAGISHAAFGGVAL
                     GFLLKVDPVLSSVFFCVASGLGIVYLSKKSHIKEDTTIGIFFASTMALGILIFGLLKG
                     VNVDLFGYLFGNILAVTLNDLYLALLVEGAVFFFVLFYYKELLLLVLDQEMAEVSGIP
                     VSMVYNLLVTLISLTIVISIKVVGIVLVSALL"
     gene            complement(646..1497)
                     /locus_tag="PK171_05530"
     CDS             complement(646..1497)
                     /locus_tag="PK171_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOT05682.1"
                     /translation="MNRAQTNSIYDVEFISVSFSYQDFEVLEDVSLQVKKGEFLAVIG
                     PNGAGKTTLLKLILGLLKPTSGKVLVFGKNINDHPNIRKRIGYVPQISKIDYSFPINV
                     EDFVLTGRYATIGPGRLPHKHDRSAVSETLEKIGLQSLSTIQIGKLSGGQRQRILIAR
                     ALINNPDMLILDEPTTAVDPKNSESLYELLLTLHQQGITILTVSHDIGVIAQYVDAIA
                     CVNRKVFVHDRPENGITDETLTEMYGREAAFFHHGIVPHIVVRRSSPVHYCSGETAED
                     DENHHKP"
     gene            1647..2063
                     /locus_tag="PK171_05535"
     CDS             1647..2063
                     /locus_tag="PK171_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOT05683.1"
                     /translation="MKSYRKELWFETSQRRSFICITPTIRDCVKESQIKEGMLLCNAM
                     HITASVFINDNEAGLHSDFENWLEKLAPEKPYSQYQHNGYEDNADAHLKRTIMGREVV
                     IAITDGKLDLGPWEEVFYGEFDGKRRKRVLVKIIGK"
     gene            2120..>2463
                     /locus_tag="PK171_05540"
     CDS             2120..>2463
                     /locus_tag="PK171_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05684.1"
                     /translation="MNNQKENSCLMLGNEAIARGAYEGGVQVGAGYPGTPSSEILEYL
                     GRYKEVHSRWCPNEKVALEVAAGVAIAGGRALATMKHVGLNVAADPLMTISYTGVRGG
                     LVIVVADDPGMHS"
BASE COUNT          812 a          449 c          527 g          675 t
ORIGIN      
        1 atgagtaaag ccgaaactaa aacgattcca acgactttta tggaaatcac aatcgtgaga
       61 gaaatcaaag ttaccagtaa gttatatacc attgataccg gaataccact tacttccgcc
      121 atttcttgat ctaataccag aagcaagagt tccttgtagt aaaaaagaac aaaaaagaaa
      181 actgctcctt cgacaagaag cgcaagataa aggtcgttca gcgtaaccgc taaaatatta
      241 ccaaataaat acccgaacaa atcaacatta acacctttca ggagaccgaa aatcaatatt
      301 cccagcgcca tcgtactggc aaagaagatt ccaattgtcg tatcttcttt aatgtgactt
      361 tttttagata aataaacaat ccctaaacca gaagcaacac aaaagaagac cgaggataat
      421 accgggtcta ctttcaaaag aaaacctaag gctacacctc caaaggcggc atgtgaaata
      481 ccagctccca taaaggacat tccttttaaa acaacgtaaa ttccgattac cgcacataag
      541 gaaccgatca aaactcctgc caacaatgca cgttgcataa aggtatagct gagaatgtcc
      601 agatcaaatc actccttgga atcgagcgct cggtacaggg tattcttaag gtttatgatg
      661 attttcatcg tcttcagcgg tttctcctga gcaataatga accggtgagg atcggcgaac
      721 cactatatga ggaacaattc cgtggtggaa gaaagcagcc tcacgaccgt acatttcagt
      781 aagggtttcg tcggtaatcc cattttcggg tcgatcatgg acaaatacct ttcgattgac
      841 gcaggcgata gcatcgacat actgagcaat gacaccgata tcgtgagaaa cggttaggat
      901 ggtgattcct tgttggtgaa gagtaagaag caattcataa agactttcac tgttttttgg
      961 atcaacagca gtggttggtt catcaaggat taacatatca ggattgttta ttaaagcccg
     1021 agcgatcagt attctttgtc gctgaccacc ggataatttg cctatttgta tggtgctcag
     1081 tgattggagg ccgatttttt ccaaagtttc acttactgcc gagcgatcgt gtttatgagg
     1141 gaggcgccca ggaccaatgg ttgcatacct accggttaaa acaaaatctt caacgttaat
     1201 tggaaaagaa tagtcgattt tactaatttg cgggacatag cctattcgtt ttcggatgtt
     1261 gggatgatcg ttgatatttt ttccgaaaac caatacctta ccggaggttg gttttaaaag
     1321 gcctaaaatg agtttcaata aagtggtttt tcctgctcca tttggcccaa tgacagcaag
     1381 aaattctccc tttttaactt gaagagaaac atcttctaaa acttcaaaat cttggtatga
     1441 aaatgaaacg gaaataaatt ccacatcata tatagaattg gtttgggccc tattcatcgg
     1501 acgaccacct ttatcatcga gctatttatg aatcatatct ggcaggagag gaaaaaaatc
     1561 aactgctctt taatgctaat caatgcttca ttttatgtca aggtcggtta caattataga
     1621 gaaaatgaag aaattgaggt ggatttatga aaagctatcg aaaagaatta tggtttgaaa
     1681 catcacaacg gagaagtttt atttgcataa ccccaacaat cagagactgt gttaaagaaa
     1741 gtcagataaa agaaggaatg ctcctttgta atgctatgca tataaccgct agtgtcttta
     1801 tcaatgataa tgaggctggt ttacatagtg actttgaaaa ttggttagaa aaattagctc
     1861 cagaaaaacc ctatagtcaa tatcaacata atggttatga agacaatgct gatgcccatc
     1921 tgaaacgaac catcatggga agggaagttg ttattgctat aaccgatgga aaattggatt
     1981 taggaccatg ggaggaagtt ttctatggtg agtttgatgg aaaaagaaga aaaagagttt
     2041 tagttaaaat tataggaaaa taacgtgaac cgaatagtat agatttatat tgccattttg
     2101 aatggaagga gcgaattcat tgaacaatca aaaagagaat tcttgcttaa tgttagggaa
     2161 tgaagcaatt gctagaggtg cctatgaagg tggggttcag gttggtgctg gttacccagg
     2221 aactcccagt agcgaaattc tcgaatatct cggtcgatat aaggaagtcc attcaaggtg
     2281 gtgccctaat gaaaaagttg ctctcgaggt agcagctggg gtagcaatag cgggagggcg
     2341 agctttagcc accatgaaac atgtaggtct taatgttgct gctgatccat tgatgacaat
     2401 ctcctatact ggtgtgagag gcggattggt tatcgtggta gctgatgatc cgggaatgca
     2461 ttc
//
LOCUS       DAOSOL010000223         2459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_42696_length_2459_cov_2.394759, whole genome shotgun sequence.
ACCESSION   DAOSOL010000223 DAOSOL010000000
VERSION     DAOSOL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42696_length_2459_cov_2.394759"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..709
                     /locus_tag="PK171_05545"
     CDS             131..709
                     /locus_tag="PK171_05545"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HOT05685.1"
                     /translation="MDLIDLIKQLNNDDVLGQVSQSMGVEPQQVKKVTQLGLPTLVEA
                     MNRNAKTQEGARSLARALEQHQDDEVDDLSKYIRHADAHDGEKILRHVFSDKSERVES
                     KIAKQTGLDMSQVMGILAQFAPLVLGLLGKEKKEKNIDYNGVANMTSSIANQLGGESG
                     SGLMGMVSQFLDSDQDGDIMDDLGGLFGGLFG"
     gene            complement(1064..>2459)
                     /locus_tag="PK171_05550"
     CDS             complement(1064..>2459)
                     /locus_tag="PK171_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05686.1"
                     /translation="MDEIFGYFPPTANPPSKLPMLTLLKQARAFGLGVVLATQNPVDL
                     DYKGLANCGTWFIGRLQTERDKMRVIEGLEGAAVSATGGFDRPQMEKILAGLGNRVFL
                     MRNVHENDSVVFQTRWAMSYLRGPLTLPQIQALSVGIKPVLPSPTPSRFETPAYEATP
                     VPHPILPPDVPEYYLRPQGTSGSVVYVPTAIGIAKMHFVSAKAKVDTWITRTLIAPLS
                     EDGRDALWEEAEIRGEIANELSTEPIGEGQFTPLPSGVTTSGIVESWKKILSTHLYQQ
                     DMLTLMQYPGLKLISKPEETEGDFRARVAQALREKRDLEVAKMKAKFAPKFQTLENKI
                     RLSQERIEREKSQAEQQKIGTYVSVGATLLGALLGRSAKVGTVGRAATAAKSMSRIGK
                     EKAEVQRAQERLEELQEQLKALQEQFDEALAPLHIDVDPMSLDLHPSIVRPRKSDIIV
                     DAMGICWVPSGKRQ"
BASE COUNT          700 a          497 c          523 g          739 t
ORIGIN      
        1 ttcttgtaca acgaaattgg cataaaagtc tattctaatt attgaataaa taggcttaaa
       61 atttcctcat tgtagtttga taataaaact gttaggatta ttggtaataa aaaagatgag
      121 gagaccttaa atggatctca tagatttgat taaacaattg aataatgacg atgttttagg
      181 tcaagtcagt caatcgatgg gcgttgagcc tcaacaggta aagaaagtta cccaattagg
      241 attgccaact ttagttgaag ccatgaatcg aaatgccaaa actcaggaag gagctcggtc
      301 tttggcaaga gctttggaac aacatcagga tgatgaggtc gatgatttat cgaagtatat
      361 ccgccatgct gatgctcacg acggcgaaaa aatccttcgc cacgtttttt ctgataaaag
      421 cgagagagtt gaaagcaaga ttgctaaaca aaccggattg gatatgagtc aggttatggg
      481 aattctggct caatttgcac cgctggtcct tggccttttg ggcaaagaaa agaaggagaa
      541 aaatatagat tataacggtg ttgccaatat gacttcctct atagccaatc aactgggagg
      601 agaatctggt tctggactaa tgggaatggt gtctcaattt ttagattccg atcaagatgg
      661 agacatcatg gatgatttag gtggattgtt tggcggtttg tttggataaa tattttttaa
      721 aaaaaagggc gcattaaatt tgcgcctttt ttttaaattt ggtcttattt gctccttatt
      781 ataagataaa aactaaaaaa tctcatctat ttcaatccca taacatttaa caaatgagat
      841 ccaattttct tcaaaaagtc ctaatgataa cattttggta ttcgctacat gaggatgaaa
      901 attcaatccc cctcacccta atgtaattcc tctctaatgc cgcaaatctt ttgccccttc
      961 tcccttgatg ggagaaggtt gggatgaggg tgaaacccga gattcagatc caagcttggt
     1021 gttatgaacc gagattaagg ttgaaatgaa tatccattgc tcttcactgc cttttcccag
     1081 aagggaccca gcaaataccc atggcatcaa caataatgtc cgattttcgg ggtcgaacga
     1141 tactgggatg caaatccaat gacattggat caacatcgat gtgaagagga gctaacgctt
     1201 cgtcaaactg ctcttgaagc gctttaagtt gttcttgcaa ttcttccaat ctttcttgag
     1261 cacgttgaac ttcagctttt tcttttccaa ttctactcat acttttagca gctgttgctg
     1321 ctcggccaac agtaccaacc ttggcgctcc tccctaataa tgctcctaat aaggtagcac
     1381 cgaccgatac ataggttcct attttctgtt gttccgcttg ggatttttct ctttcaatcc
     1441 gctcctggga gaggcgaatt ttattttcaa gagtttgaaa ttttggagca aatttagcct
     1501 tcattttcgc tacttccaaa tcacgttttt ctcgcaaagc ttgagcaact cgagcgcgaa
     1561 aatcgccttc ggtttcttct ggttttgata tcaatttcaa ccctggatac tgcatgagag
     1621 taagcatgtc ctgctgatat aaatgggttg ataatatctt tttccaactt tcaactattc
     1681 cggaagtggt cactcctgaa ggtagtggag taaattgtcc ctcaccgatt ggttcggtag
     1741 agagttcatt tgctatttct ccacgtatct ccgcttcttc ccataatgca tcccgaccat
     1801 cttcagaaag tggtgcaatc aaagtgcggg taatccaggt atcaactttg gctttggcac
     1861 tgacgaaatg cattttggca ataccgattg cagttggaac ataaactacc gatcctgaag
     1921 ttccttgagg acgcaggtaa tattctggaa catcgggagg gaggatggga tgtggaactg
     1981 gagttgcttc ataagctggt gtttcaaatc gtgatggtgt cggcgagggt agaactggtt
     2041 ttataccaac agaaagagcc tgaatttgag gcaaagtcaa aggacctcga agatagctca
     2101 ttgcccatcg agtttggaaa acaacagaat cattttcatg aacattccgc atgagaaata
     2161 cccggtttcc taaaccggcg agaatttttt ccatttgtgg ccgatcaaaa cccccggtgg
     2221 cacttactgc agcaccttcc aatccctcaa ttaccctcat tttatcccgt tcggtttgca
     2281 accgtccgat aaaccaagtt ccacagttgg caagtccttt ataatcgagg tccactgggt
     2341 tttgggtagc caggacaacc cccagtccga aagcacgagc ttgtttaagc agagtcaaca
     2401 ttgggagttt tgatggtgga ttagccgtag gtggaaagta accgaatatt tcatccata
//
LOCUS       DAOSOL010000224         2424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_43460_length_2424_cov_3.619671, whole genome shotgun sequence.
ACCESSION   DAOSOL010000224 DAOSOL010000000
VERSION     DAOSOL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43460_length_2424_cov_3.619671"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PK171_05555"
     CDS             <1..308
                     /locus_tag="PK171_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT05687.1"
                     /translation="VAQAAYAATKAAVMNFSRALSREFAPLGINVNSICPGIVDTPLG
                     QVNLNDPVKKEKFIKMTPKGRISIPEDLAGITAFLASDDADFIVGQAINVDGGILYY"
     gene            384..1892
                     /gene="xylB"
                     /locus_tag="PK171_05560"
     CDS             384..1892
                     /gene="xylB"
                     /locus_tag="PK171_05560"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOT05688.1"
                     /translation="MPAEYILGIDLGTSSLKASLLDLYNTKIISASVELTVSYPDSYS
                     AEQNPYDWWEAFRTLIQKIKQLIGSLDSIKAIGLSGQMLGLINLDGKGQPLRPALIWC
                     DQRSFIELNEIREMIGEEKLLQNTANTPLTGYWLPKLLWLRKHQKDILQKSRHYLLPK
                     DYLRYRLTDEYITEVSDASGTLLFDVAQRTWSRKLIKDFNFDFSCFPRVVESTEITGY
                     VSLKVSQETGLPEGIPVVGGGGDQSSGGIGLGVIDKNIMSCVLGTSGVVMAQTEEAKM
                     DQKNRGLHSFCYSIPGKWFLMGCTLAAGGSYRWLRHSLLHLQKDLSYDHLNSLAGEVG
                     PGSDQLVFLPYLIGERTPHSDPNARGVYFGLSYQHDIRHIIRATIEGVAFSQKESIEI
                     LKDFGLEADHLIVSGGAARSQLWCQIMADVAGIEVVTTNIDDPASVGAAFIAGVGTGI
                     FDSFQSGCGRFIRHGDVLEPSSENNRLYKTLFEKYQKLYQVLKNYYQDYQVT"
     gene            1974..>2424
                     /locus_tag="PK171_05565"
     CDS             1974..>2424
                     /locus_tag="PK171_05565"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:NF014753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HOT05689.1"
                     /translation="MKKFINKYENLIEEMVEGYVLANSNKVKRLPNERVLVYSNAPIQ
                     GKVGVITGGGSGHKPAFIGYIGKGLLDAVAVGDIFAAPPVQRCYEAIKAADGGKGVLV
                     CIGNYSGDTMNFGIAAELAQDEGIPVEILVVNDDVASSPKDRMDNRRG"
BASE COUNT          732 a          415 c          537 g          740 t
ORIGIN      
        1 atgttgccca agctgcctat gctgcaacca aagcagctgt gatgaacttt tcccgggcac
       61 tatcaagaga atttgcgccc ttgggaatca acgttaactc tatttgccca ggtattgtgg
      121 atactccatt aggtcaggtt aatttaaatg atccggtcaa gaaagagaaa ttcatcaaaa
      181 tgactcctaa gggaaggatt agtattccag aagatttagc tggtattact gcttttctgg
      241 ccagcgatga tgccgatttt attgtgggac aggcaatcaa tgttgatgga ggaatcttat
      301 attattaatt attgaattgt atatttgtat attttttatt ttttgggggg attaatctaa
      361 aaaaatgaag ggagctgtcg ctattgcctg cggaatacat tttaggtatt gatttgggta
      421 catcatccct aaaggcatcg cttctcgact tatataatac caagataata tcagcatcag
      481 ttgaactgac tgtatcatat ccagattctt attcggccga gcaaaatccc tatgattggt
      541 gggaagcttt tcgaacttta attcagaaaa taaaacaact aatcggaagc ctagattcta
      601 taaaagctat tggtttatca ggacaaatgc ttggtttaat aaatcttgac ggaaaaggtc
      661 aacccctcag gccagcattg atttggtgtg atcagagaag ctttattgaa ttaaatgaaa
      721 taagagaaat gattggcgaa gagaagctat tacaaaatac tgccaacaca ccgctcacag
      781 gatattggct ccccaaactt ttatggcttc gtaaacacca aaaggatatt ttacaaaaat
      841 ccagacatta tctccttccc aaagattatc tccgttatcg gttaaccgat gaatatatca
      901 ctgaagtatc agatgcctct ggtactttac tctttgatgt cgcgcaacgc acctggtcaa
      961 gaaagcttat caaagatttt aattttgatt tttcttgttt tcctcgagtg gttgaatcaa
     1021 ccgaaataac tggatatgtt tctttaaaag tgtcacaaga gacgggcctt ccagaaggaa
     1081 tcccagttgt tggtggtggt ggcgaccagt cttccggagg tattggtctt ggagttattg
     1141 ataaaaacat tatgtcttgt gtattgggaa cttctggtgt tgtaatggct caaactgaag
     1201 aagcaaaaat ggaccaaaaa aatagaggtt tgcattcctt ttgttattct atcccgggaa
     1261 aatggttttt aatggggtgt accttagctg caggtggttc gtaccgttgg cttcgtcatt
     1321 ctttacttca tctccaaaag gacttgagtt acgatcacct caactcatta gctggtgaag
     1381 ttggaccagg aagtgatcag ctggttttcc tgccttatct tataggagaa cgaacccctc
     1441 attccgatcc taatgctcgt ggtgtttatt ttggtttaag ctaccaacac gacatccggc
     1501 atataattcg ggcaaccata gaaggagttg ctttcagcca aaaagaatcg atagaaattc
     1561 tgaaagattt tgggcttgaa gctgaccact tgattgtttc gggtggagcc gctaggagcc
     1621 aattgtggtg tcaaattatg gctgacgttg ctggtattga agtcgtaacc accaatattg
     1681 atgatccagc ttcggttgga gcggctttta ttgctggagt cggaactggt atttttgatt
     1741 catttcaaag cggatgcgga aggtttattc gacatgggga tgtattagaa ccatcttcag
     1801 aaaataatcg gctttataag acattatttg aaaaatacca aaaactttac caagtattga
     1861 aaaattatta ccaagattat caagtaacat gagaatttta aaaaaattac ggtctattta
     1921 tcaataaatt ggaagctaaa attaggtaaa tattttacaa gggggtcaac tacgtgaaga
     1981 aatttattaa taaatatgaa aatcttatcg aagagatggt agaaggatat gtactggcaa
     2041 attcaaataa agtgaaacgt ctccctaatg aaagagtttt agtctattcc aacgctccca
     2101 ttcaagggaa agtaggtgta ataactggag ggggctcagg gcataagcca gcttttattg
     2161 gatatattgg gaaaggtctt ttagatgccg tagcagttgg ggatatcttt gccgctcccc
     2221 ccgttcagcg ttgctatgaa gccattaaag cagctgatgg tggcaaaggg gtattggtat
     2281 gtattgggaa ttattctggt gacactatga actttggaat agctgcagag ttagctcaag
     2341 atgaaggaat tccggtagaa atcttagtag taaatgatga cgttgcgtca tctccaaagg
     2401 atcggatgga taatcgacga ggag
//
LOCUS       DAOSOL010000225         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_43965_length_2405_cov_3.470638, whole genome shotgun sequence.
ACCESSION   DAOSOL010000225 DAOSOL010000000
VERSION     DAOSOL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43965_length_2405_cov_3.470638"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1285
                     /locus_tag="PK171_05570"
     CDS             134..1285
                     /locus_tag="PK171_05570"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05690.1"
                     /translation="MNIITREDIQNLLKKQEGICVSLYAPMEKSGDTQQNPIRFKNLL
                     SIAEKHLIDQGFRENELKDFLSPIKKFQQDSLFWEHQSDGLAVFLSKNCFHYYRQPYR
                     FKELALVADHFHIKPLLPLLVGDGRFFILAISQNSIKLMQGTRYSVNEIDLEGVPEGL
                     ASVIRQEGMEKLLQFHTGTPTTRGDRSAIFFGHGAGYNTKEIIQRYFQIVNKSLHDFI
                     RNEKIPLVLASVDYLLPIYREVNTYPYLLEEGIEGNPENLTKDKLHEKAWKIVEPIFY
                     KAQREALEKCEAYLQKADKTVSINIEDIIPAAYHGRIDTLFVAIGIEKWGRFDPDNNQ
                     IIIHEKNELGDTDLLDFCANQVLRHSGSVFALKTDAIPGNSIIAAIYRY"
     gene            complement(1750..>2405)
                     /locus_tag="PK171_05575"
     CDS             complement(1750..>2405)
                     /locus_tag="PK171_05575"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT05691.1"
                     /translation="ETDFTFGFDSAVWVATDAIDRLHTTAQAHHRVMVVEVMGRYAGW
                     IALYSGIAGGGDVILIPEIPYDIENICTFIRKRIAAGKKFSIVVVAEGSKPQGGDFTV
                     LRKVEESHDQIRLGGVGHVISEQIEGKTGIETRAVILGHLLRGGSPTPYDRVLASRFG
                     TAAVDLVAQEQFGLFPALRGNEIKPVPIKKAIEKLKTIPLDHSLLKLARSTGTCLGE"
BASE COUNT          760 a          510 c          434 g          701 t
ORIGIN      
        1 taccgatttc acaactcgtg ataaaataat ggtaactaca atttttatat attctaaaat
       61 aacctaagct gtgtaattaa gtaaatggtt tctcccgcaa tatttgaaaa ctttttaata
      121 ggagggaatg ggtatgaata tcattacgag agaagatatt caaaaccttc tcaaaaagca
      181 ggaggggata tgcgtctctc tttatgctcc catggaaaaa agtggtgata cccaacaaaa
      241 cccgatccga tttaaaaacc tcttatctat tgctgagaag catcttattg accaagggtt
      301 tcgagaaaac gagttaaaag attttctttc accaataaaa aaatttcagc aagatagtct
      361 tttctgggaa catcaaagtg atggtttagc tgtatttctt tcaaaaaatt gtttccatta
      421 ctaccgtcaa ccctatcgtt tcaaagagct tgctttggtt gctgatcact ttcatattaa
      481 gcctcttctc ccccttttgg tgggtgatgg ccgttttttc atcttagcga ttagtcaaaa
      541 ttccatcaag cttatgcagg gtactcgata ttcggttaat gaaatcgatc ttgaaggagt
      601 tcccgaaggt ttagccagcg tcattcgtca agagggaatg gaaaaacttt tacagtttca
      661 taccggtacc ccaaccacaa ggggggatcg atcagcaata ttttttggtc atggagccgg
      721 ctacaacacc aaagagataa ttcaacgtta ttttcagata gttaataaat cccttcacga
      781 tttcattcgg aatgaaaaaa tccctctggt tcttgccagt gttgactatt tgctcccaat
      841 ctatcgggaa gtaaatacct atccgtattt actggaagaa ggaattgagg gtaacccgga
      901 aaatctcacc aaagataagc ttcacgaaaa agcctggaaa attgtcgaac ctatttttta
      961 caaagctcag agggaagcac tcgaaaaatg tgaagcctat cttcaaaaag ccgataaaac
     1021 agtttcaatt aatatagagg atattattcc agctgcttat catggacgaa ttgatactct
     1081 ttttgttgct attggtattg aaaaatgggg gcgttttgat ccagataaca atcaaatcat
     1141 tattcatgaa aaaaatgaac tgggggatac tgatttactt gatttttgtg ccaatcaagt
     1201 cttaagacat agtggatcag tttttgcctt aaaaaccgac gctattccgg gaaattcaat
     1261 aattgccgca atttatagat attaaatatt aatacctttc cccctatagc aataactccc
     1321 tttattaatt ccttacacaa attgcaatct tttcaactaa gaattccggt agagttaatg
     1381 ctcctctgtc ctgaaaatac cgaaataagt aaaaagtgat gaaacaaaaa agaaaaaggc
     1441 acacccccct gaatcccccc tcaatggggg aataaaatca acaattcccc tccgtggagg
     1501 ggtgccgctt tcgcggcggg gagggtgcct tttgtttttt tattgattac cctgagacaa
     1561 tgaataagga acattaaaaa attttgataa atgagattgc cacgtcgcat aagacgttcc
     1621 tcgcaatgac ggagtaggaa taattcaaat ccccctaacc ccctttttga aagggggata
     1681 acgattcctg agtcagtatt tttcaccctc atccttttct tctcccatca atggagaagg
     1741 tgcatattcc tattccccta aacaggttcc agttgaacga gcaagtttca ataaagaatg
     1801 atccaaaggt atggttttaa gcttttcaat ggcttttttt atcggaactg gcttaatttc
     1861 attgcctcgc aaagctggaa acaatccaaa ttgttcttga gcgactagat ccactgccgc
     1921 tgttccaaaa cgactggcga gtactcgatc atagggagtt ggagaaccac ctctcaataa
     1981 atgaccaaga ataactgccc gagtttcaat gccagttttt ccctcgatct gttcactaat
     2041 aacatgtcca actccgccca agcgaatttg gtcatgactc tcttccactt tccgtaacac
     2101 agtaaaatct ccaccttgag gttttgatcc ctcggcaaca actacgatgc taaacttttt
     2161 ccccgcagct attcgtttac gaataaaagt gcaaatattt tcaatatcat aaggaatttc
     2221 aggaatgaga atgacatcac caccaccagc tattccagaa tacaacgcaa tccatccagc
     2281 ataccggccc attacttcta ccaccataac tcgatgatgg gcttgagcag tagtatgaag
     2341 tcgatcaatc gcatcggtag caacccatac tgcagagtca aaaccaaaag taaaatcggt
     2401 ttccg
//
LOCUS       DAOSOL010000226         2396 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_44190_length_2396_cov_4.150363, whole genome shotgun sequence.
ACCESSION   DAOSOL010000226 DAOSOL010000000
VERSION     DAOSOL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44190_length_2396_cov_4.150363"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PK171_05580"
     CDS             <1..245
                     /locus_tag="PK171_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014525059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05692.1"
                     /translation="VKSDDIVGRVKAYEAIVKGQNVLKPSVPESFKVLVKELQSLCLD
                     VKVFDSRKKEISIEQMEDADDDIPGLGVNLQGREKK"
     gene            264..>2396
                     /locus_tag="PK171_05585"
     CDS             264..>2396
                     /locus_tag="PK171_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05693.1"
                     /translation="MIDVNDLGAMQIGLSSPEQIRKWSRGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECYCGKYKRIRHKGVVCDRCGVEITRSSVRRARLGHIELAAPVS
                     HIWYFKSIPSKMALLLGVLPKNLEKVLYFASGRKKEDCYKVIEPGSTDLEPETVIRDT
                     EYRIHQKYDPNFKAETAHRITEVHSLPFSVGDELSTKELTRFRTKYKESFTIEELENN
                     RYEVIDVRVFPYQRDEEISESEVYQLREVYGDLFEEEILTDTAEEPCYLVIDPGSTGL
                     KKAQMILETEAKLLPQYDTEFKSGVGAEAVREILRTLDVQTLARSLRKELKEATGQKA
                     KKIIKRLQVVESFCKSGNRPEWMILEVVPVLPPDLRPMVQLDGGRFATSDLNDLYRRV
                     INRNNRLKRLLQLGAPDIIVKNEKRMLQEAVDALFDNGRRGRPVLGPGNRPLKSLSDM
                     LRGKKGRFRQNLLGKRVDYSGRSVIVAGPQLNFDQCGLPKKMALELFKPFIMKGLVDK
                     GHAHNIKSAKRMVEKTRDEVWEVLEEVIAEHPVLLNRAPTLHRLSVQAFQPVLVEGNA
                     IHIHPMVCPAFNADFDGDQMAVHVPLSAEAQAESEILMLSSHNILSPASGRPIAVPTQ
                     DMVLGCYYLTLMKKVSKGTILKRYLDMDEALLAFEYKQVDLHEPIFIREVQEKDSNVA
                     MTTIGRVIFNQVLPQGMPYYNDPMTKKKL"
BASE COUNT          742 a          457 c          553 g          644 t
ORIGIN      
        1 cagtgaaatc cgacgatatt gtcggaagag taaaagctta tgaagcaatt gtcaaaggtc
       61 aaaatgtgct caagccgtcg gttcccgaat catttaaagt attggttaag gaattgcaaa
      121 gcttgtgtct cgatgtgaaa gtgtttgatt ccaggaaaaa ggaaatttcc atcgaacaga
      181 tggaagatgc cgatgatgat atacctggat taggagttaa tctgcaggga cgagagaaaa
      241 aataaaagaa gggggaaaaa cccttgatag atgtcaatga tcttggagct atgcaaattg
      301 gtttatcttc tcccgagcaa atccgtaaat ggtctcgagg agaagtaaaa aaacccgaaa
      361 ccattaatta tcggaccctc aaaccggaaa aagacggttt gttctgtgag aagatttttg
      421 gtccaaccaa agactgggaa tgttattgcg gaaagtataa aagaattcgt cataaaggag
      481 tggtctgtga ccgttgtgga gttgaaatca ctcgatcttc ggttagaaga gcccgtctgg
      541 gacatatcga attagctgct ccggtttcgc acatttggta ttttaaaagt attcccagta
      601 aaatggctct ccttttgggt gttcttccca aaaacctgga gaaagttctc tattttgcct
      661 caggaagaaa aaaagaagac tgctataaag ttattgaacc gggttcgacc gatcttgaac
      721 cagaaactgt tatacgagac accgaatacc ggatccatca aaaatatgat ccgaacttta
      781 aagccgagac cgctcatcgg attaccgaag tccacagtct tcccttttca gttggcgatg
      841 aactctccac caaggaattg actcggtttc gtacaaaata caaagagagt tttacgatag
      901 aagaattaga aaataatcgc tatgaagtga tcgatgttcg ggtttttcct taccaacggg
      961 atgaagaaat ttccgaatcg gaagtttatc aattgcgtga ggtgtatggc gacctctttg
     1021 aagaagaaat cttgaccgat actgctgagg aaccttgcta tctggttatc gatcccggat
     1081 caactggttt aaagaaagcc caaatgatat tagaaaccga agcgaaattg cttccccaat
     1141 atgataccga atttaaaagc ggcgttggag ccgaagcagt cagagagatt cttagaactt
     1201 tggatgttca gactctggct cgaagtttgc ggaaagaact caaagaagca accggtcaaa
     1261 aggcgaagaa aatcatcaag agattacaag tcgtcgaatc cttttgtaaa tcaggaaatc
     1321 gaccggagtg gatgatattg gaagtggttc cggtacttcc gcccgatttg cgtcccatgg
     1381 tccagttaga tggtggtcgg tttgcaacct ccgatttgaa tgacctctat cggagagtga
     1441 ttaaccgaaa caatcgtttg aaaagattgc tacagcttgg agcccctgac attatagtaa
     1501 aaaatgaaaa acgcatgctt caggaagccg ttgatgcatt atttgataat ggacggagag
     1561 gacgtccggt attaggaccg ggcaatcgcc cgcttaaatc actcagcgat atgttgcgag
     1621 gaaagaaagg tcgttttcgc caaaatttat tagggaaaag ggttgattat tccggtcgtt
     1681 cggttatcgt tgccggacct cagttaaatt tcgatcaatg cggacttccc aagaaaatgg
     1741 cattagaact atttaagcct tttattatga aaggtttagt tgataaaggt catgctcata
     1801 atattaaaag tgccaaaaga atggtagaga aaacccgcga tgaagtttgg gaggtattag
     1861 aagaagttat tgctgagcat ccagttctcc tgaaccgagc accaaccctt caccggttga
     1921 gcgttcaagc ctttcaacca gtgctggtag aaggcaatgc cattcatatt catcctatgg
     1981 tttgtccagc ttttaacgcc gatttcgatg gtgatcagat ggcagttcat gttccgcttt
     2041 ctgcagaagc tcaagctgaa tcggaaattt tgatgctttc ttctcacaat atcctttctc
     2101 cggcatcagg tcgaccgata gcagtaccaa ctcaagatat ggtgttaggc tgttactacc
     2161 taactttgat gaaaaaagta tccaaaggaa caattctgaa gcgatatctc gatatggatg
     2221 aagcactcct ggcttttgaa tacaaacaag tcgatcttca tgagccgatt ttcatccggg
     2281 aagttcagga gaaagactct aatgtagcta tgacgacaat tggtcgagtt atttttaatc
     2341 aggttcttcc tcaggggatg ccttattaca atgatccgat gaccaaaaag aaacta
//
LOCUS       DAOSOL010000227         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_44351_length_2390_cov_3.764882, whole genome shotgun sequence.
ACCESSION   DAOSOL010000227 DAOSOL010000000
VERSION     DAOSOL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44351_length_2390_cov_3.764882"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            531..929
                     /locus_tag="PK171_05590"
     CDS             531..929
                     /locus_tag="PK171_05590"
                     /inference="COORDINATES: protein motif:HMM:NF014088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOT05694.1"
                     /translation="MSTYLLFLKLPKMINISVGKLGQFSFPAGQYLYVGRAKKNLEAR
                     LKRHQRKNKTLHWHVDYLLQYAILETIITYDVNDECGLARYLTCQPGITLPVHKFGSS
                     DCHCESHLFHLVGSLCSLGLPPGYAVFLNS"
     gene            1058..>2390
                     /locus_tag="PK171_05595"
     CDS             1058..>2390
                     /locus_tag="PK171_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05695.1"
                     /translation="MKRMVVLVYLFISIWIIAGYCVPGEALEKLTQVNQENSQLVETF
                     SNRTTSAALRPEDFTYLGAFRLPDDGDRPNTFAYGGEAMTFNPSGDPNGPSDGFPGSL
                     FVMGHNRIPYGELPEGNKIAEITIPVPKKSRLVSDLNQAEFLQPFSNIDAGYFFNLDE
                     IPRVGMQYLDNPATGPIIHITWGQHFQEDSAFMVPSQAWFATDLSSPNTQGSWYIGNQ
                     SFYSTNDYLFEIPLEWAEPYLGGFPLATGRFRDGGWSGMGPCLFAYCPWIDDHGTPAE
                     SGSRLEEKVLLLYESSLNTPDITRCLNDYQHGDEWTGGAWITTPSGKNAVLFAGTKSS
                     GDLYWYGYLHPDGPEYACVEKEFIGQFTLCRLADGTPYPDERSAVCSNPTSNRGWWSS
                     RFNARFILYDPMDLIKVATGELESYQPQPYTFLDIDDHLFLNPMGIELEMLG"
BASE COUNT          683 a          469 c          500 g          738 t
ORIGIN      
        1 gaataataac cttcccagtg agatgaaggc aaaaaggttc gttagagtcc gtggtctttt
       61 tttggccttt attttcttcg attaaccaca ttgtagcacc ttttaaggat gaaaacacta
      121 attaaatccg taattgcgag gagcgtcctt tgcgacgtgg caatctcatt cctaaaaaca
      181 gtgagtcgtg ggcagtgaat cgtgaaccga ataaaaaaaa taaaaattga tgaactttgg
      241 tagtgagaaa cttataaaaa aagcatctcc ccttagccaa atggagaata attttgaaag
      301 aggagatcct cacggcttca taaagcgaag cctcaggatg acgaattaaa aagcaccctc
      361 cccgccgtaa aacggcaccc ctccaaggag gggaattgtt aaatttattc ccccattgag
      421 aagaattagg gggatgtacc tttatctctt ttgcttattc ttttttcaac aaatttggta
      481 ttttcaggat agatatataa tatctaaaac atctaccaaa agataagacc atgagcacct
      541 atctgctctt tttaaaatta ccgaagatga tcaatatatc ggtaggcaaa ctcggtcaat
      601 tttcatttcc tgctggtcaa tatctttatg tgggaagagc caagaaaaat ctcgaagccc
      661 gcttaaaaag acatcaaaga aaaaataaaa ctcttcactg gcacgttgat tatcttcttc
      721 agtatgcaat attggagaca attattactt atgatgtaaa tgatgaatgt ggtttggcaa
      781 gatatttaac ctgccaaccg ggaataactt tacccgtcca caaattcggt tcctcagact
      841 gtcactgtga atcgcatttg tttcacttgg ttggaagtct ctgttcactg ggtttacctc
      901 caggttacgc ggtttttctt aatagttaag tcaatggttg ttatggaaat ctaaatttcc
      961 ctaattgttt aaattgagca actttaaaaa tttggtttct ttccgatcgc ttttaataaa
     1021 aaattaaaat ttggatttgc taaaggatgt gaaccaaatg aaaagaatgg tagttttggt
     1081 ttatttgttt atatctattt ggataatagc gggctattgt gttccaggag aagcattaga
     1141 aaaactaaca caagtgaatc aagagaattc ccagttggtt gaaacattca gcaatcgaac
     1201 cacatcagca gcattacgtc cagaagactt tacctatttg ggtgctttcc gtcttcccga
     1261 tgatggcgac agaccaaaca cctttgccta tggtggagaa gcaatgactt tcaatccatc
     1321 tggtgatccc aatggtccga gtgatggctt cccaggatct ttatttgtca tgggccataa
     1381 tcgaatacct tatggagaat tacccgaggg gaataaaatt gctgaaatca ccatcccggt
     1441 gccaaaaaag tctcggttgg tttctgatct caaccaggct gagtttctcc agcctttttc
     1501 gaatattgat gctggttatt ttttcaattt ggacgaaata cctcgagttg gtatgcaata
     1561 tcttgataat ccagcgactg gcccaattat ccatattacc tggggccagc attttcaaga
     1621 agattcggct tttatggtcc cctctcaggc ttggtttgcc actgatttgt catcaccgaa
     1681 tacgcaaggt tcctggtata ttgggaatca atcattttac agtaccaatg attatctttt
     1741 tgagattcca cttgagtggg ctgagcccta tttgggagga ttcccgcttg ctaccgggcg
     1801 cttccgagat gggggatggt caggcatggg accttgctta tttgcttatt gcccttggat
     1861 tgatgaccat ggaacaccag ctgaatctgg ttcacgattg gaggaaaaag tattgttgct
     1921 ttatgagagt tcattaaaca cccccgatat cactcggtgt ttgaatgatt atcaacatgg
     1981 tgatgaatgg acaggtgggg cttggattac aactccatca gggaaaaacg cagttctttt
     2041 tgcagggacc aaatccagcg gtgatcttta ttggtatggt tatctccatc ctgatgggcc
     2101 ggaatacgct tgtgtagaaa aagagtttat cggtcagttt actctttgcc gattggctga
     2161 tggaactccc taccccgatg agcgttcggc agtatgttcc aatcctactt ctaatcgagg
     2221 atggtggagt tcacgtttca atgcccgttt tattctttat gatccaatgg atttaataaa
     2281 ggttgctact ggagaacttg aatcttacca gcctcaacct tatacattcc tggatattga
     2341 tgatcatctc ttcctcaatc caatgggaat cgaactggaa atgttaggga
//
LOCUS       DAOSOL010000228         2390 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_44366_length_2390_cov_2.465525, whole genome shotgun sequence.
ACCESSION   DAOSOL010000228 DAOSOL010000000
VERSION     DAOSOL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44366_length_2390_cov_2.465525"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PK171_05600"
     CDS             <1..491
                     /locus_tag="PK171_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05696.1"
                     /translation="FGLIYELPVYANITLAILKKMIRNGVLQIKKEHEVAKHYVHALQ
                     IKPPNIFREILYLSGGNQQKVLLARWLAREPKILIMDEPTRGIDVGAKFEIRNFVKEM
                     VREGLTVIYITSDPLEAIEMGDRVVVLRNGQIVTTFNNPLGLDKSEILAAASGVHYGR
                     EK"
     gene            488..1531
                     /locus_tag="PK171_05605"
     CDS             488..1531
                     /locus_tag="PK171_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05697.1"
                     /translation="MMDKNHLTKNEQKERVLLPSYQPGNLVRLVLLKGGSLIGLIGLS
                     AFFALSSQHFLTISNVLNIFRQVSVVLIAACAQTMVIISAGIDLSVGPLIALGGSVGA
                     VAMSYWGWDFLSGAFLGVVIAMLAGFTNGILIAKGKIPDFIGTLGMLGAFRGVALLIT
                     DGLPVPSHFTATALKGYLPPALVWLGAGDIFQIPAPMIIALIMIGITWFILMRTSFGR
                     SIYAVGGNKEAARASGINVEWTKIWVYGLSGLYCGIGGLVLTGRMNSANALMAEGIEL
                     QTIAAVVIGGTNLFGGQGTIFGTFIGAILMGVLANGLNLLNVEPFWQRVINGIIIVSV
                     VIFDQWRRRKLIS"
     gene            1758..>2390
                     /gene="msrA"
                     /locus_tag="PK171_05610"
     CDS             1758..>2390
                     /gene="msrA"
                     /locus_tag="PK171_05610"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOT05698.1"
                     /translation="MKGLLFALLIILPFIFGSYTLAQESNQNPSKLEKATLAGGCFWC
                     LESAMEKLEGVIEAISGYTGGHTENPTYEQVSSGTTGHYEAVEVTFDPKKISYREILD
                     AFWKSINPTDPFGQFVDQGSQYRTAIFYHNQEQKTIAEQSKEDLKKSGKFNDPIVTEI
                     LPVLVFYPAEEYHQGYYKKSNNRYEVYRSFSGRDQFQEKYWKDDENVTENQ"
BASE COUNT          648 a          514 c          525 g          703 t
ORIGIN      
        1 gttttggcct gatttacgaa ttacccgttt atgccaacat cactttggcg atactcaaaa
       61 aaatgattcg aaacggtgtt cttcagatca aaaaagaaca tgaggttgcc aagcattacg
      121 ttcacgctct gcaaattaaa cccccgaata ttttccgtga aattctctat ttaagcggtg
      181 gtaaccaaca aaaagttctt cttgcccgtt ggttggcacg agaacccaaa atcctgatca
      241 tggacgaacc aactcgtggg atcgatgtgg gagccaaatt tgaaatccgt aattttgtca
      301 aagagatggt ccgggaaggt ttaaccgtca tttatatcac atcggatcct ttggaagcca
      361 ttgaaatggg ggatcgggta gttgtgcttc gaaacggaca aatagtgacc acctttaata
      421 atcctctggg attagataaa tcagaaattt tagcagccgc cagtggagtt cattatggga
      481 gggaaaaatg atggataaga atcatttgac caagaatgaa caaaaagaga gagttttatt
      541 accatcttac caacctggga atctggtacg tctggttctc ctaaagggtg gttctttaat
      601 cggcttgatt ggcttaagtg ctttttttgc cttatcatct caacattttc ttactatcag
      661 taatgtgctt aatattttcc gtcaggtttc cgtcgtttta atagccgcct gtgcccaaac
      721 catggtgatc atttctgccg gtattgacct ttcggtagga cctctgatag ctctgggagg
      781 aagcgtcggt gcggtcgcca tgagttactg gggatgggat tttctttctg gtgctttttt
      841 gggtgttgtc attgccatgc tagcgggttt taccaatgga attttaatcg ccaaaggaaa
      901 aatccccgat ttcatcggta ccttaggtat gcttggtgct ttccggggtg tggctttatt
      961 gattaccgat ggattgccgg ttccctctca cttcactgca actgctttga aaggatacct
     1021 tcctccagca ctagtttggt tgggagccgg tgacatcttc caaatcccgg caccgatgat
     1081 catcgctctt attatgatag gaatcacctg gtttatattg atgcgaacca gtttcggccg
     1141 ctctatctat gcggttggcg gcaacaagga agctgcccgt gcttcgggca ttaacgtcga
     1201 atggaccaaa atttgggtgt atggcctatc cggtctctat tgtggaattg gtggattagt
     1261 tctcaccgga cggatgaact ccgcgaatgc tttgatggct gaaggtatcg agcttcagac
     1321 cattgctgca gttgtcattg gaggaaccaa tctgtttggc ggccagggaa ctatatttgg
     1381 aacttttatc ggtgccattc tgatgggagt gctggcaaac ggcttaaacc ttcttaatgt
     1441 tgaacccttc tggcaacggg tcatcaacgg aatcatcatt gtctccgtgg tgatctttga
     1501 ccaatggaga agaagaaaac tcatttcgta aaaaaaatta cctttcttct atcaatgaat
     1561 aattttttgg tatcatcctg agcggctctc tttcgcgcac gcttcttatt cttttgattt
     1621 ttttctcccc ctcaccccct cattccctct ccccctcgga gctttctgtc aatactctcc
     1681 cttaaggaat aaaaaaccaa aaaattcatt acacaatatt aaaagaaatt tcgcagataa
     1741 tgaatgaggt gatcaacatg aaagggctcc tgtttgcatt acttatcatt ttgcccttta
     1801 tttttggaag ttatacatta gcacaagaat caaatcagaa tccctcgaaa ctggaaaaag
     1861 ctaccctggc aggtggttgt ttctggtgtt tggagagtgc catggaaaaa ctggagggag
     1921 tcatcgaagc catctctggc tataccggag gccataccga aaatcccact tatgagcaag
     1981 tcagttcggg aaccaccggc cattatgaag cggttgaggt tacttttgat ccaaaaaaaa
     2041 tatcctatcg ggaaatcttg gacgccttct ggaaaagtat caatccgacc gatccctttg
     2101 gtcaattcgt tgatcagggt tcacaatacc gaactgccat tttctaccac aaccaagaac
     2161 agaaaactat cgccgagcag tcaaaagaag atttaaaaaa gtcgggaaaa ttcaatgatc
     2221 ccattgtaac cgagattctc ccggttttag ttttctaccc ggccgaagaa tatcatcagg
     2281 gttactacaa aaagtccaat aatcgctacg aagtctaccg ttcattttcc ggacgggatc
     2341 aatttcaaga aaaatactgg aaggatgatg aaaacgtgac tgaaaatcaa
//
LOCUS       DAOSOL010000229         2389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_44385_length_2389_cov_3.246358, whole genome shotgun sequence.
ACCESSION   DAOSOL010000229 DAOSOL010000000
VERSION     DAOSOL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44385_length_2389_cov_3.246358"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(605..1708)
                     /locus_tag="PK171_05615"
     CDS             complement(605..1708)
                     /locus_tag="PK171_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOT05699.1"
                     /translation="MAHIESSGNIPKKMKAVVNYAPGDFRLEEVDVPEIGPGEVLIKV
                     GGCGICAGDVKTFHGAARIWGGDGQPAYVKPPVIPGHEFYGQVVALGEGASKKYGLKL
                     GDWATSEQIVPCGVCRYCLRGEYWMCEVHNIYGYQKHDAEGGFAEYMRFPATARNHKL
                     PEGFPLHMAPYIEPLGCAIHAVERADIQLDDVVVIAGIGPLGFGMLQTARLKNPKLLI
                     AIDAKEKRLNLAKELGADLVMNPLKEDVVAKVKDLTDGYGCDVYIHASGNPKGVHQGM
                     NMIRKLGRYVEFSVFNEPTLLDWSIIGDVKEIEIRGAHLSPYTYPAAIRFIQEGKVKA
                     EDIITHEFPLVEFKKGMELAEKGDESIKVVLIP"
     gene            complement(1894..>2389)
                     /locus_tag="PK171_05620"
     CDS             complement(1894..>2389)
                     /locus_tag="PK171_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05700.1"
                     /translation="FAQLLVTTGSEALNDQVIADWQRKPLAFPDARVRWKEITMTPNK
                     APMELRLVSDSLALLQEYGEKIHQLLLEIDEQQLQAKYRFKHRTLPFLIIIAILLFSA
                     LYLIYPCLYGFIETLIEFIEEQPPFIHDQKTPNIMVYEIDQRGKLMNQIIRNTKYLQF
                     DHLS"
BASE COUNT          702 a          516 c          461 g          710 t
ORIGIN      
        1 taaagaatta ggctaccata aaatgcgtct tgataccatt tcgaccatga aagaggcaat
       61 cactctttac cagtcgctgg gcttttatga aattgagcct tattataata atccggttga
      121 aggcgctcat tatatggaaa taaatctgat tggataggat aaaaaagagg atagatactt
      181 ttttcttaaa atccattgac gggttaaata aatcaagctt taacaaaaga attttaaaaa
      241 agccgtcatc ctgaggcttc gctttttaaa gccgtgagga tctcatcttt tataatttat
      301 ggaatcattg aatcggggaa cgatgaaact ggaaaggatg agattctcac gtcgtccggg
      361 aaagacactg gactcctcag aacgtagaaa atgttgctct ctcgcgcctt ctcccctccg
      421 gtctttgtta catccgatta atttagtgac ataccaagac aggtcgaatc ttaggttttc
      481 atcctcatca attgccacca agttgcatgt ggatctatcc agtaattaaa gtatttagta
      541 aacaaaaaaa ttgcccctct ttttgataga gaggggcaat tggccattag acttatataa
      601 atggttatgg aatcaatacc actttaattg actcgtcacc tttttctgcc aattccattc
      661 cttttttgaa ctcaacgagc ggaaattcat gggttatgat atcttcggct ttcactttgc
      721 cttcttgaat aaagcggata gcagctggat aggtgtaggg actcaaatga gcgcctcgaa
      781 tttcaatttc tttcacatct ccaataatgg accagtcaag gagagttggt tcattgaaaa
      841 cactaaactc aacatatctt cccaattttc gaatcatatt cattccttga tgaactccct
      901 tgggattacc actggcatga atatagacat cgcagccata gccatcggtc agatctttca
      961 ctttagccac cacatcttct tttaatggat tcataactaa atctgcacct aattctttag
     1021 ctaaatttaa tcttttttct tttgcgtcga ttgcaatcag gagtttcgga tttttaagac
     1081 gagcagtctg aagcatacca aatccaagag gtccaatgcc ggcaatcacc acaacgtcat
     1141 cgagttggat atcagctctc tcaacggcat ggatagcgca ccctaacggt tcaatatagg
     1201 gggccatatg aagggggaag ccttcgggta gtttgtgatt gcgagcagta gctggaaatc
     1261 tcatatactc ggcaaaacca ccttcggcat catgtttctg atagccgtaa atattatgaa
     1321 cttcacacat ccagtattct cctcgaaggc aataacgaca aacaccacaa ggaacaattt
     1381 gctcggaagt tgcccaatca ccgagcttca aaccatactt tttgctggcg ccttctccta
     1441 atgccaccac ttgaccataa aactcgtgac cggggataac tggaggtttc acataagccg
     1501 gttgaccatc tcctccccag attcgtgcgg caccatggaa agtttttaca tctcccgcac
     1561 atattccaca accaccaact tttatcaaaa cttctcctgg cccaatttct ggaacatcaa
     1621 cttcttctaa gcggaagtcg ccaggagcat agttgactac cgccttcatc ttttttggaa
     1681 tgtttccaga tgattcgata tgtgccataa caaggtacct ccatgattct tattttttta
     1741 tcaattactt tcccccaatg attttctctg ttcaccttaa tattatatac aaatttgggg
     1801 aagaagacag aaataattaa aagatgagat cctcacgctc tcgaaaaacc agggctcagg
     1861 atgaccgatt aaagaattta ttagatggta ttttcaggat agatggtcaa actgtaggta
     1921 ttttgtgttt ctaataatct gattcattag ctttcccctt tgatctatct cataaaccat
     1981 aatattagga gttttttgat catgaataaa aggaggttgt tcttcaataa attcaattaa
     2041 agtttctatg aaaccataga ggcatggata aatgagatag agagcagaaa aaagcagtat
     2101 agcgataatg ataaggaagg gcagtgtacg atgtttaaaa cgatacttgg cttgtaactg
     2161 ttgttcatca atttcaagta atagttggtg gattttttca ccatactcct gcaacagggc
     2221 caggctatcg ctcaccaggc gcaattccat gggcgccttg ttcggggtca tggtgatttc
     2281 tttccagcgg acccgggcat ccggaaacgc caagggctta cgttgccagt cggcaatcac
     2341 ttgatcgttc aaggcttcgg agcccgtcgt gacgagcaac tgcgcaaaa
//
LOCUS       DAOSOL010000230         2316 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_46288_length_2316_cov_2.965060, whole genome shotgun sequence.
ACCESSION   DAOSOL010000230 DAOSOL010000000
VERSION     DAOSOL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46288_length_2316_cov_2.965060"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PK171_05625"
     CDS             complement(<1..194)
                     /locus_tag="PK171_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOT05701.1"
                     /translation="MEQAVYDSYENMLFNLDNAAKNLGLNYDDYVALRYPERELTVSI
                     PVIMDNGHIEVFTGYRVQYS"
     gene            complement(935..2131)
                     /locus_tag="PK171_05630"
     CDS             complement(935..2131)
                     /locus_tag="PK171_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT05702.1"
                     /translation="MNQNGFYQVDYSKLEIPEVKIGMLGYGFMGQVHSNAFIKVPYSF
                     SNPVSRPVLLAICGRNQKKVEATARRFGYKEYCLDWQLLAKDPRIDIFDNCTPDDWHY
                     LPSIVAMENGKHVICEKPLALKLEEAFQMAQKARETGVKTMCCYNYRFLPAVQLARQL
                     IEQGILGKIYQFRAKYLQQQGHNPFEHIEKVWYAHGTQSGTLMGIGSHILDMAHFLVG
                     EITAVSGLKKTYNQIRKDSSGKAHSISADECTIAILEFKNGAVGTIESSGVSTGRKNY
                     QGWEINGSKGSIVFDLEDLNHLQVYSTDANPPEIRGFSNVLVTDSEHPLPINYLPSGH
                     NQGWEYGHVHALHHFLDCVVNDKPVEPLGASFQDGYRVQVVIEAINQSSAKGKRVEIK
                     EVGQDI"
BASE COUNT          703 a          481 c          390 g          742 t
ORIGIN      
        1 cttgaatatt gaactcgata cccggtaaat acttcaatat gaccattatc cattatgact
       61 ggtatagaaa cggttaactc cctttctgga taacgaaggg caacatagtc atcataattt
      121 aaacctaggt tttttgctgc attgtcaaga ttaaaaagca tattttcata cgaatcataa
      181 actgcttgtt ccatttttta attacccctc caaaaatgat ttaaatcaat tttttattga
      241 aggaggaaat atttacatat atttttatta ttatctcagt attaataacc cgtaataaaa
      301 ctactttaat aaaataatag atttatcgca atcataagtc aatcccgtta atatgcttat
      361 tttgaataat tggcatatat tattggaaat attttgctat aaatggaata aacaaagtga
      421 acatgggcag gaataagggt tcatatttta agcaaaaaat gaatgagaaa aaacaatttc
      481 tctgaggcta tataaccatc ttccccgaaa gaattattta ttctgaaaaa tatataattg
      541 gttataggta atgggtttag agctactaaa tctgatctaa gtctgggttt tcacccgcac
      601 tccaatcttc tcccatcaag agaaaaagaa tcgttttgat atcatcacga attttctctt
      661 tttgaaggcg tgaggatctc atcttttaag ttttttcttc ataaatactt taaagaatga
      721 gattctcacg tcgtccggca aaaacaccgg actcctcaga atgactaagt ggatggtaga
      781 gattgccacg tcgcacaaga cgctcctcgc aatgacggat ttagataggt attttcatcc
      841 tcatctggtg ctgctaagca gcatgaatac ctatcttcaa attaatgttt tctgcttatc
      901 ctcaattgac actttcctct tgagaaatat aggttcaaat atcctgtcca acctctttaa
      961 tttctactct tttgcctttt gctgaggatt gattgatggc ttcaatcact acttgaaccc
     1021 gatacccatc ttgaaaggaa gctcccaatg gttccaccgg tttgtcgttg actacacaat
     1081 ctaaaaaatg atgaagagcg tgaacatgtc catattccca accttgatta tgaccagaag
     1141 gcaaataatt tattggtaag gggtgctcgc tatcagtaac taatacatta ctaaaacccc
     1201 ggatttcagg tggattagca tcggtagagt agacttgcaa gtggtttaaa tcttcaagat
     1261 caaaaactat actccctttg gaaccattaa tttcccaacc ttggtagttt tttcttccgg
     1321 tacttactcc tgatgattct atcgtcccaa ctgcaccatt tttaaattct aaaatagcaa
     1381 tggtgcattc atctgcagaa atcgagtggg ctttccccga actatctttt cgtatctgat
     1441 tataggtctt cttcaatcca ctgactgctg ttatctcgcc gactaaaaaa tgtgccatat
     1501 caaggatatg gcttccaatc cccataaggg ttcccgattg agttccatga gcgtaccaca
     1561 ccttttcaat gtgctcaaaa ggattgtgtc cttgttgttg taagtatttg gctcggaatt
     1621 ggtatatttt ccccaggatt ccctgttcaa tcagttggcg agccaattga accgcgggga
     1681 gaaaacgata attatagcag cacatcgtct tgactccagt ttcccgagct ttctgagcca
     1741 tttggaaagc ctcttcaagc tttaaagcta agggcttttc acatataaca tgttttccat
     1801 tctccatagc tactatggaa ggaagataat gccagtcatc tggggtacag ttatcaaaaa
     1861 tatcaattcg gggatcctta gccagtagtt gccaatcaag acaatactcc ttgtaaccaa
     1921 accggcgtgc tgttgcttca actttttttt ggttacgacc acagattgcc aataggaccg
     1981 gacgggacac tggattggaa aaactgtaag gaaccttaat aaatgcattg gagtgaactt
     2041 gccccataaa accatatccc aacatgccaa ttttcacttc cggaatttcc agttttgagt
     2101 aatcaacctg ataaaatcca ttttgattca tagcattcac ccatcacgct gttatttttt
     2161 gtaaatacct gatgctctca gcaacagctt gatcttcatt accataattt ggatctaaat
     2221 agtcaatttc caaggtcaaa cttccttgat aatgattctc cttaaaaacc tgaaccaacg
     2281 caggaaagtc gataatgcct cttcctgccg gcacgc
//
LOCUS       DAOSOL010000231         2313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_46370_length_2313_cov_3.592560, whole genome shotgun sequence.
ACCESSION   DAOSOL010000231 DAOSOL010000000
VERSION     DAOSOL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46370_length_2313_cov_3.592560"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="PK171_05635"
     CDS             <1..1250
                     /locus_tag="PK171_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT05703.1"
                     /translation="HIDCDVVVIGGGPAGMASALSAWNHGARKIFILERNDYLGGILN
                     QCIHPGFGLQIFGEELTGPEYAARYIDQLAKTSIEVMTETTVLDLTPNKKITAVNPKG
                     VWEFYSESVILSMGCREKTRGMIFIPGFRPAGIYTAGLAQYLVNIEGYLPGKKVVILG
                     SGDIGLIMARRLTLEGVKVEAVVEVRPSLSGLIRNYVQCLLDFELPLLLSHTVTNIFG
                     KERVEGVEISRVDQSWQPIPGTERLLSCDTLLLSVGLLPENEISRKAGVSLCHFSHGP
                     FVDENFSTDLDGIYACGNVASVFDLVDYVTLVSQKAGQSAAENRDKKPIKYQLNPGSG
                     VGLVIPQRVRQVNDGWLFIRPDQSFRDGEVQFRTQGKVFDQKRVAVCRPSEMVRYDLK
                     NIHWPEEGGEIIVEIVGSSVEGI"
     gene            1213..1608
                     /locus_tag="PK171_05640"
     CDS             1213..1608
                     /locus_tag="PK171_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOT05704.1"
                     /translation="MLKLSEAQSKESKQLICTSCPMGCSLRVEKDGEKIVVTGAKCKR
                     GSQYAQDEMLNPKRTVTTTVVVLNGEIRRLPVRTKKPFPLRRVSELIQFLSTIEVEAP
                     VQVGQVIATNCLGEGVDIIATRTILRRSK"
BASE COUNT          723 a          420 c          492 g          678 t
ORIGIN      
        1 accacattga ttgtgatgtc gtcgttattg ggggagggcc agcaggtatg gcatcagcac
       61 tatctgcttg gaaccacggt gcccggaaaa tatttatctt agaaagaaat gattacttgg
      121 gaggaatatt gaatcagtgt attcatcccg gttttggttt gcaaatattt ggagaagaac
      181 tgactggtcc tgaatacgct gcccgatata ttgatcaatt agccaaaacc tcaatagagg
      241 tgatgactga aacaaccgta cttgacctta ccccaaataa aaaaataact gcggttaacc
      301 caaaaggtgt ttgggaattc tatagtgaat cagtcatact cagcatggga tgtcgagaaa
      361 aaaccagagg gatgattttt atccctggat ttcgtccagc tggaatctat actgcaggct
      421 tagctcagta tctagtcaat attgaaggtt atttgccggg gaagaaagtg gttattttag
      481 gatcagggga tattggctta attatggccc gcaggttaac ccttgaaggt gttaaagtag
      541 aagcggtggt agaggtacgg ccttcattaa gcggtcttat ccggaattat gtgcagtgtt
      601 tgcttgactt tgagcttcct ctattacttt ctcacacagt aaccaacatt tttggtaaag
      661 aacgagtcga gggagtagaa ataagtcgag ttgatcaatc ttggcaacca attccaggaa
      721 ccgaaaggtt attaagctgt gacacccttc ttctttcggt aggactcctt cctgaaaatg
      781 aaatatctcg aaaagctggt gtttctctgt gtcattttag tcatgggcct tttgttgatg
      841 aaaatttctc tactgacctt gatgggatat atgcctgtgg aaatgtcgct tcagttttcg
      901 atttagtcga ttatgtcact ttggtgtcac aaaaagccgg tcagtcagcc gctgaaaata
      961 gagataaaaa accaataaag tatcaattga atcccgggtc aggagtaggt ttagttatcc
     1021 cacaacgagt tcggcaagtt aatgatggtt ggttatttat tcgtcctgac caaagtttcc
     1081 gtgatgggga agttcaattt agaactcaag gaaaagtatt tgatcaaaag agagtagccg
     1141 tctgtcgccc tagtgagatg gttcgctatg accttaaaaa tatacattgg cctgaagaag
     1201 gaggagaaat cattgttgaa attgtcggaa gctcagtcga aggaatctaa acaactcatt
     1261 tgtacttcct gtcctatggg atgttctctc cgagttgaaa aagatggaga aaaaattgta
     1321 gttactggtg caaaatgcaa acggggaagt cagtacgcac aagatgaaat gctcaatcca
     1381 aaaagaacag tgacaacaac agttgttgtt ttgaacgggg aaatccgacg gcttccagtt
     1441 cgaactaaaa aaccctttcc ccttcgacgc gtctcagaat tgattcaatt tctttcgact
     1501 atagaagtcg aggcaccagt tcaggttggt caagttattg caaccaattg tttgggagaa
     1561 ggtgttgata taatagctac tcgaacgatt ttgaggcgat ccaaatgatg gaaatattca
     1621 ttgccttata gttataacaa gaaattggag ataatcatta agcaggtggt gagaaattcc
     1681 cagttctttt tcaaatttga tttgccctga ctcagagata aatatcaatc cttataatgc
     1741 tgttataaat aaacccccaa ccataagtca tttctcgatt tttcctcata ttttgaactt
     1801 ttaaaaatgt tcattttgta atttgacaaa cgaaaaaata ttcgatataa attaattata
     1861 agttattgag aaaatgatat tgagaaattt ctcaatttgt aatgaatatc tccccaaaaa
     1921 gtcttaatat aagaaaagaa cttcatgcta cgttcgcagt accagataaa aatgaaaata
     1981 gctatctcaa tccgtcattg cgaggagctc ggcgacgtgg caatctcatt tagctattct
     2041 ttcattctaa ggaatccgga attttttact ggacattata aggatctcat tcaattcgat
     2101 tccacaattc tataatttct caattctaca aataataaag atgactcatg agattgccac
     2161 gacctcaaaa accaggtctc gcaatgacga ctcaaagttc cttctccctt gatgagggag
     2221 aaggtgagga tgaggatgaa agcccagaat gacgaatgaa aagcactctc cccgctgtaa
     2281 acggcacccc tccaaggagg agaattgttc aat
//
LOCUS       DAOSOL010000232         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_46965_length_2292_cov_3.438534, whole genome shotgun sequence.
ACCESSION   DAOSOL010000232 DAOSOL010000000
VERSION     DAOSOL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46965_length_2292_cov_3.438534"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1143)
                     /locus_tag="PK171_05645"
     CDS             complement(133..1143)
                     /locus_tag="PK171_05645"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOT05705.1"
                     /translation="MKACLFEAKEKYVICDIEKPVPQKDEVLIRVKVVGICGTDIHIL
                     KGEYFSDFPLIAGHEFSGEVVEVGEEVTQFQPGDRVTADPNIFCDKCYFCKINKNNHC
                     LDSHVVGVTQNGAFAEYVAVSEKGIFSIPDHLSYSEAALAEPLACVVYGIRRSGIKPG
                     EKVLIFGAGAIGLLLMSLLKMNGASQVVMVDISQKKLDFAKKMGASEVILNNEDGEKK
                     LKAIAPFGFELVADATGSPRVMERELQFVEPDGTFLVFGVAPIGAMMKVEPYDIFRRD
                     IRIIGSYAVKKTMQYSINLLASGKIQVKNLISSTHPLEDFEKGILDFYHDPDHMKIQI
                     IP"
     gene            complement(1124..>2292)
                     /locus_tag="PK171_05650"
     CDS             complement(1124..>2292)
                     /locus_tag="PK171_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05706.1"
                     /translation="LAKKTRSVTEELNARTMPEFLEARYRSKTLKYLAAVIIFILLVP
                     YSASVYMGLSYLFDVVMGIPYVYALLLMAILTGVYLVMGGYFAVSITTLVQGGIMFFG
                     SIYMVLYLLSRPEVGGMSQVVAKMSAIDPKLVSIVGPPGFIGLFGLVVLTSFGPWGLP
                     QMVQKFYSIKNQKVIFTATIVTTVFALVCSFSAYFSGALTHLFFKELPTLNGVTNPDL
                     LMPNLLIQYMPTAFSIIFLLLVFSASMSTLSSLVLVSSSAITIDLLPKKYGKDNVTVM
                     RILCILFIFLSLIIAISEVTFIVNLMSISWGATAGSFAAPYVYGLFWKKANRQGALAS
                     MITGLAFSLILSWAFGFKSSIVPFIGSLAILIPFGVLPIVSILTGGNKNESLPI"
BASE COUNT          776 a          462 c          427 g          627 t
ORIGIN      
        1 taaataggtt agattcctgg attagttaca gtgtggcttc atttgatgat aatgaaaaat
       61 atttatatgc cggtcaatct cctcctttag caaaggggga tttgaattta ttgataacgc
      121 atagttacta aattatggaa tgatttgaat tttcatatgg tcggggtcgt gataaaaatc
      181 cagtattcct ttttcaaaat cttctaaagg atgtgttgaa gagataagat ttttaacctg
      241 gatttttccc gaggcgagga gattaataga atattgcata gtttttttca cagcgtaaga
      301 gccgatgata cgaatatctc gacgaaagat atcataaggc tcgactttca tcatagcacc
      361 aataggggcg actccaaaca ctaaaaaggt tccatcaggc tcaacaaatt gtagctctct
      421 ttccataact cggggtgatc cagtagcatc ggcaaccaac tcaaacccaa agggagcaat
      481 cgcttttaac tttttttccc catcttcatt gttgagaatc acttccgatg cacccatctt
      541 tttagcaaaa tcaagtttct tttggctaat gtcaaccatg actacctggg aagcaccatt
      601 cattttaagt agtgacatta aaagtaaacc aattgctcca gctccaaaga ttaaaacttt
      661 ttctccaggt ttaatcccac ttcttcgaat tccataaacg acacaggcca gcggttcggc
      721 aagcgccgct tcagaataac tcaagtgatc aggaattgaa aagatcccct tttcggaaac
      781 ggctacatat tcagcaaagg ctccattttg agtcactcct accacatggc tgtctaaaca
      841 atggttattt ttattgattt tacaaaaata acatttatca caaaatatat taggatcagc
      901 agtaacccga tcacctggtt ggaactgagt gacttcttct ccgacctcaa ccacttctcc
      961 cgaaaactca tgtccagcta ttaaaggaaa gtcagagaaa tattcacctt taagaatatg
     1021 tatatctgtg ccacaaattc caacaacttt cactcgaata aggacttcgt ctttttgagg
     1081 gacaggtttt tcaatatcgc aaatcacata tttttctttg gcttcaaata ggcaggcttt
     1141 catttttatt tcctccagtt aatattgata ctatgggaag aactccaaaa ggaattaaaa
     1201 tagccagtga tccaataaat gggacaatgc ttgatttaaa tccaaatgcc caggatagga
     1261 ttaaagaaaa tgctaaacca gtgatcatgc tggctaaagc accctggcga ttggcttttt
     1321 tccagaaaag accataaaca taaggcgcag cgaaactacc cgcagttgct ccccaagaaa
     1381 tcgacattaa atttactata aaggttactt cagatatggc aatgattaag gataaaaata
     1441 taaataagat gcataaaatc ctcatcaccg tcacattgtc ttttccatat tttttgggta
     1501 aaaggtcaat ggtgattgct gaagacgaca ccagaactaa tgatgatagg gttgacattg
     1561 atgcagaaaa gaccaacaga agaaagataa tggaaaaagc agtgggcatg tattggatta
     1621 acaaattggg cataaggaga tcgggattgg ttactccatt caacgttggg agctctttga
     1681 agaagagatg agttaacgct ccggaaaaat aagcactaaa gctacaaacc agagcgaaaa
     1741 cggtggttac aatagtagca gtaaatatca ctttttggtt ttttattgaa taaaacttct
     1801 gaaccatttg aggtagtccc cagggaccaa agctggttaa aaccactaaa ccaaaaagac
     1861 caatgaatcc cggagggcca acaatactaa ccagttttgg atcaatggca ctcattttgg
     1921 ccactacctg actcataccg ccgacttcag gtcgggataa caaatataaa accatgtaaa
     1981 tcgaaccaaa aaacatgata ccaccctgga ccaaggtggt aatgctcacc gcgaagtacc
     2041 cacccataac tagataaaca ccggtgagaa tagccatgag taatagagcg taaacatagg
     2101 gaatgcccat aaccacatca aacaaataac tcaatcccat ataaacagaa gcagaataag
     2161 gaaccaataa gatgaaaata ataaccgcag caagatattt taatgttttg cttctgtagc
     2221 gagcttccag aaattctggc atagtccgag cgttgagttc ctcggttacg gagcgagttt
     2281 ttttggctaa aa
//
LOCUS       DAOSOL010000233         2292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_46968_length_2292_cov_3.156460, whole genome shotgun sequence.
ACCESSION   DAOSOL010000233 DAOSOL010000000
VERSION     DAOSOL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46968_length_2292_cov_3.156460"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="PK171_05655"
     CDS             complement(<1..1467)
                     /locus_tag="PK171_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOT05707.1"
                     /translation="MIRKKQISFGFIIVFMLLFFSLPAIASDISDAIVKVYAVSDPPD
                     YLNPWNMMGPSSVTGSGAVIEGNRILTNAHVVSDLTFIQVRRYGDTQRYPARLLAISH
                     QCDLALITVDDPSFFQGIDPLPTGDLPATNQEVLVYGFPMGGDTLSITKGVVSRIEHQ
                     PYVHSSITLLAGQIDAAINPGNSGGPVIVDGKIVGVVMQGIPSSQNIGYMVPAPIIKH
                     FFEDLSDEQLDGFPNLGVYMQYMENPDIREKYTMPVNITGVLVNQVIPGSSADGIIQP
                     GDVILSIDDSPIANDGTVEFRAKERTNLAYIVQKHQIGDDLNLKILRNGKVQNLSVNL
                     SSSYEKNWLIPYEQYETLPTYYIYGGLVFSPLSINLFNIWGSSWFNNAPKELVALLAN
                     NIPTVEGEQVIILLRVLPASVNDGYQNYAPWIVKKVNGKEVLNMKELVQTIENDDDNL
                     FVILENNTNQQIVLNREKVKQANPQILATYKIREDRSLD"
     gene            1759..1971
                     /locus_tag="PK171_05660"
     CDS             1759..1971
                     /locus_tag="PK171_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05708.1"
                     /translation="MANPAERIGSILGEIERTKGMVKSPDRRSAYLTPLYTQLAREII
                     DEVKKQVKEDIMKELRNEIRGTSFIE"
BASE COUNT          723 a          410 c          443 g          716 t
ORIGIN      
        1 atccagtgag cgatcttctc gaatcttata agtggcaagg atttgtggat tggcttgttt
       61 tactttttca cgattcagga caatctgttg attcgtgtta ttttctaaaa tgacaaataa
      121 attatcatca tcgttttcaa tagtttgaac gagttctttc atattaagaa cttccttccc
      181 atttactttt tttacaatcc atggagcata gttttgatac ccgtcattaa ctgatgctgg
      241 taaaactcgt aaaagaataa tgacttgttc gccttcgact gtagggatgt tattggcgag
      301 caatgcaacc aattctttag gagcattatt aaaccagctc gaaccccata tattaaataa
      361 gttaatgctt aaaggagaaa acaccaagcc accataaatg taataggtcg gtaaggtttc
      421 atattgctca taggggatta accaattttt ttcataagag ctgctaagat ttaccgatag
      481 gttttgaact ttgccgttgc gcaaaatttt gagatttaaa tcatcgccaa tttgatgttt
      541 ttgaacaata taagctaaat tggttctttc ctttgcacga aattctactg ttccatcgtt
      601 ggcaattggt gagtcatcta tcgataggat aacatccccg ggttgaatga taccatcagc
      661 agatgaacca ggaataactt gatttaccag aaccccggtt atatttactg gcatggtata
      721 tttttctcga atatcggggt tttccatgta ttgcatataa actccgaggt tggggaaacc
      781 atccagttgc tcatcagaaa ggtcttcaaa aaaatgtttt attatcggcg ctggaaccat
      841 atatccaatg ttctgtgaac tgggaatccc ttgcatgact acaccgacaa tttttccgtc
      901 tacaattacc ggtccaccac tattccctgg attaatagct gcatctatct ggccggctag
      961 aagtgtgatg gaactatgca cataaggttg gtgttcaata cgcgatacaa ctcctttggt
     1021 aatgctcagc gtatcgcctc ccatggggaa accatatacc aatacttctt ggttggttgc
     1081 tggaaggtct ccagtgggca gagggtcaat accttggaaa aaactgggat catcaacggt
     1141 tatcaaggca agatcgcact gatgagatat cgccagcaga cgagcagggt atcgttgcgt
     1201 atcgccatac cttcgaactt ggataaaggt gagatcactt acaacatgag cattggtgag
     1261 gatacggttc ccctcaatga ctgctcctga accagtgacc gaagagggac ccatcatatt
     1321 ccagggattt aagtaatcag gaggatcgga tacagcatat accttaacaa tggcatcgga
     1381 tatatcagac gctatcgctg gtaaggaaaa aaataataac ataaagacaa ttataaaacc
     1441 gaatgaaatc tgttttttcc taatcatcaa tttccatctc cttgacagtt gatttgtatc
     1501 ctgcttttta gttaagaata ttcatatcat acctgatatt gtttatcatg actatcaaaa
     1561 agagttcaaa taaaaaagtc tttcatttaa aatattacta atagataaga attactaaaa
     1621 aatggtcatt ttctcttaat ttaaaaatta aaatacgata aaattgaaag agattaaata
     1681 taactatctt cctgtttttt tacattttta aaaactgcta gaataagaat taattttcaa
     1741 taaatgaagg aggatgaaat ggccaatcca gccgaaagaa ttgggagtat attaggagaa
     1801 attgaacgga ccaaggggat ggtgaagtca cctgatagga ggtctgctta tttgaccccg
     1861 ctttatactc aattagctcg agaaattatt gatgaggtaa agaagcaggt aaaagaagat
     1921 atcatgaaag agctgcggaa tgaaataaga ggaacctcat tcattgaata aatttttatt
     1981 attctaaatt tttgtctaaa taatttaata ataagaaata tttatggaat tttaccataa
     2041 tcccttataa gaattggtaa gaatgcgatg ctttttctga tacctatcaa ctatttgatt
     2101 agtggataga gcctcatact actttcatgg cacgagtaga ggatgataat actattcaga
     2161 aatcaatatc cccctttgca aaggtggatg aagggggatt tgaatttttt cctgttccgt
     2221 cattgcgagg agcgcagcga cgtggcaatc tctgccaccc agtttgtcat tctgaggagt
     2281 ccggtgtctt ct
//
LOCUS       DAOSOL010000234         2291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_47000_length_2291_cov_2.646243, whole genome shotgun sequence.
ACCESSION   DAOSOL010000234 DAOSOL010000000
VERSION     DAOSOL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47000_length_2291_cov_2.646243"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1664)
                     /locus_tag="PK171_05665"
     CDS             complement(249..1664)
                     /locus_tag="PK171_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT05709.1"
                     /translation="MKQFKPIDNQDIQYFKTIVGEQFVSNDPEVLKNYSKDETPDETL
                     RLLPEIVIKPTTSGEIQLITRYCFEKNIAITPRGAGSGLSGGSIPAYGGIVLSLERMN
                     RILEIDIENLIVVVEPGVITNDINRRLKSYGLFYAGYPMSLETCQIGGNVAENAGGGK
                     AVKYGVTGHYVRGVEFVTASGELVHFGGKIYKDVSAYDIVHLMVGSEGTLGIITKIYL
                     KVIPLPRERIVLLAPFDDVNQALRLPVILLKEMAVVPTSLEFMNHESLDFTYNYCNRS
                     YPFPEAKSHILVEIDGDDEDRVFSLSEKIGNRLFDMGAKEVYMADNSVLIEEIWKMRR
                     AVPEAIHLALPVEMNEDISLPIQKIGDLLLIVEKLSQQYHLFSPVYGHIGDGNLHVTL
                     APKRKKDWRKDEPVIRKTFYGKVKELGGVLSGEHGVGLKRKNYLSQFYSLEEINLMRN
                     IKKAFDPKGILNPGKIFPDSE"
     gene            complement(1651..>2291)
                     /locus_tag="PK171_05670"
     CDS             complement(1651..>2291)
                     /locus_tag="PK171_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT05710.1"
                     /translation="ADCTGFDIDSKDGALLQIRPAIGGNIMATIKTRTYPQMATVRPN
                     TFKPVQIIFPDSTLVIEEDNTTYSSGFLKNEFIEDDHNLDIAKAKILCSGGRGLKNAK
                     NFSLLEELAQCLNGCVGGSRVAVEQGWTSFPHQVGLSGKTVSPEFYIALGISGAIQHL
                     AGMQTSRYIIAINRDPEAQIFQLADLGIVGDVLEILPQVIQILKQRKKNETI"
BASE COUNT          650 a          512 c          406 g          723 t
ORIGIN      
        1 gggcgtgagg atctcatctt ttaggttctt tcttcataaa tactttaaag gatgaaattc
       61 tcacgtcgca taagacgctc ctcagaatga ctcattgagt aaatgagatt accacgtcgc
      121 tgcgctcctc acgatgacgg aacagtaaaa attcaaatcc ccctttatcc ctttttacta
      181 aaaggggaga ttggttcctg aataggtatt gtcatcctca gttggtgccg tgaagtgaca
      241 tgggtggctt attctgaatc aggaaatatt ttcccgggat tgagaattcc ttttggatca
      301 aaagcttttt taatatttct catcaagttt atttcttcta gtgaataaaa ttgtgagaga
      361 tagttttttc ttttcaatcc aactccatgc tctccactta acacccctcc caattctttt
      421 acctttccat aaaacgtttt gcggataact ggttcgtctt tcctccagtc tttttttcgt
      481 tttggcgcta aagtcacatg taaattccca tctccaatat gaccatacac cggggaaaat
      541 aaatgatact gttggctcaa tttttcaaca ataagcagaa ggtctccgat tttctggatt
      601 ggaagtgata tgtcttcgtt catttccaca ggaagagcaa ggtgaatggc ttctggcacg
      661 gctcgtctca tcttccagat ttcttcaatt aaaaccgagt tatcagccat gtagacttct
      721 tttgctccca tatcaaataa ccgattccct attttttctg agagcgaaaa cactctatct
      781 tcatcatcac catctatctc aaccaagata tgagacttcg cttccgggaa tggataagaa
      841 cggttgcaat aattataggt aaaatccagg ctttcatgat tcatgaattc taatgatgtg
      901 ggtaccactg ccatttcttt cagcaaaatg actggtagtc gtaaagcttg attcacatca
      961 tcaaaagggg ccagcaaaac aattctttcc ctgggaaggg gaatgacttt aagatagatt
     1021 ttggtaatga ttccgagagt tccctccgat cccaccataa gatgaacgat atcgtaagcg
     1081 gaaacatctt tataaatttt ccctccgaaa tggaccaatt ctcccgatgc agtaacaaac
     1141 tccactcccc gaacataatg accggtaaca ccatacttaa ctgccttgcc ccctccagca
     1201 ttttcagcga catttcctcc gatctgacag gtttctaaac tcatcggata accagcgtag
     1261 aataaaccat agctttttaa tcggcggtta atgtcgttag ttatgactcc aggttcgaca
     1321 actacgatca ggttttcaat atcgatttct aaaatccgat tcattctctc taaagataac
     1381 acaataccac cataggctgg aatcgacccc cccgaaagac cactccctgc accccgcggt
     1441 gttattgcaa tattcttttc aaaacaatac cgggttataa gctggatttc accacttgtt
     1501 gttggtttta taactatttc gggcaataat cgcagggttt catcaggagt ttcatccttt
     1561 gagtaattct ttaagacttc tggatcatta gatacgaatt gctctccaac aatcgtttta
     1621 aaatactgta tatcctggtt atcgatcggt ttaaattgtt tcattttttt tcctttgttt
     1681 taaaatttga atgacttgag gaaggatttc taatacatct ccaactatac ctaagtcggc
     1741 aagttgaaag atttgcgctt ctggatctcg gttaattgct atgatgtaac gagaggtttg
     1801 catcccagcc aaatgctgaa ttgcccctga tataccaaga gcaatataaa attcgggtga
     1861 tacagttttc ccacttaaac caacttgatg gggaaaagag gtccatcctt gctcgaccgc
     1921 cactcgactt ccccccacac aaccgtttaa acattgtgct aattcttcaa ggagagaaaa
     1981 gtttttcgca ttttttaatc cccgaccacc actacataga atcttcgctt tggcaatatc
     2041 aagattatga tcatcctcga taaattcatt ttttaagaat ccactcgagt aggtcgtatt
     2101 atcttcctca atcacaagag ttgaatcagg aaatataatt tgaacaggtt taaaggtatt
     2161 gggacgaacc gtcgccattt gaggataggt tcgagttttt atagttgcca ttatatttcc
     2221 accaatagct ggtcggattt gcaacagagc cccgtcttta gagtcgatat caaaaccagt
     2281 acaatcagcg g
//
LOCUS       DAOSOL010000235         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_47360_length_2277_cov_4.415842, whole genome shotgun sequence.
ACCESSION   DAOSOL010000235 DAOSOL010000000
VERSION     DAOSOL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47360_length_2277_cov_4.415842"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1205)
                     /locus_tag="PK171_05675"
     CDS             complement(186..1205)
                     /locus_tag="PK171_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05711.1"
                     /translation="MKNIPNHYQDNQVAIQDIPFKRKFTLRYEITVILALAIIFVFFS
                     ILSPNFLGPLILPGILLIGAELGILALGQTFVIISGEIDLSIASVYGWAAVISTLLSN
                     YGFPYPVGLIIAIAFGCMIGYFNGLITQKFKIPALIATLGMMWILRGNLIGLFGGSFI
                     SYKGKESILLQSLGGRIGYFPRLFFWFVGIALLLNYILYHTRLGNWIFATGGNRETAR
                     ALGVNTTRSKIASFVICSALAAFAGGVYIGRIDWFLTRIGMSSMGYGLEIEAIAASIM
                     GGTAFTGGKGSIIAVSIAAFAFSSFKSGLILIGATGYWIDAAVAVLLILFCLLQRMGR
                     SSNHT"
     gene            complement(1250..2233)
                     /locus_tag="PK171_05680"
     CDS             complement(1250..2233)
                     /locus_tag="PK171_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05712.1"
                     /translation="MPEQTHLKPPKSAKSILMRSETIVVILLMTFFILFSRISAGFFS
                     SSMLNVIFLTGSELGIIALGVTLLIISGEMDLSVASVFVFSNYIVLISNQFGLPILVS
                     FILGIIFGIGAGFLNGFFTMKFKIPSFVVTLGFMIFWRAVLAGLTNGEPTYYEGVPTR
                     LFKVLGGQIGFIPNQFIWFCILAVVFSIVLNRTKFGNWIMATGGSIQTARALGVNIFR
                     TKMICFMISSSLAAFAGASFLVRSSYLDPIVATGMELEAVAAAVIGGTVLTGGIGSII
                     GTSICAFLLKVVQVGIATYGIRVEYYNVLVGILLMIAAVINSALIKKLLKT"
BASE COUNT          808 a          489 c          368 g          612 t
ORIGIN      
        1 tagatgataa tatgccctct tttttttgca aagcatgttc aaacaactca ggtatgctta
       61 aatgataaaa tatttgcttg ggattaaata cacctgatct ctcgaatgaa tagtgggcct
      121 tctgcatcaa gttctcacct tcccaaaaaa gaattttttc aattctacca tatatttcct
      181 atacatcaag tatgattgct gctgcgtccc attcgttgga gaaggcaaaa aaggatgaga
      241 agaactgcta ctgcggcatc aatccagtat ccagttgctc caattaatat caaaccactt
      301 ttaaaagaag aaaaagcaaa tgctgcaatt gaaactgcaa taatacttcc tttccctcca
      361 gtgaaagccg ttcctcccat aatggatgct gcaatcgcct ctatttctaa accataaccc
      421 atactactca taccaatccg agttaaaaac caatcgatcc gtccaatata aaccccaccc
      481 gcaaaagctg ctaaagcaga acaaataacg aaagaagcaa ttttactccg ggtggtgttc
      541 acacccaacg ctcgagccgt ttcacgattt ccaccggtag caaatatcca attccccaat
      601 cgagtatgat ataaaatata attaagcagc aaagcaattc caacaaacca gaaaaaaagg
      661 cgagggaaat agccaattcg accaccaagt gattgcaata ggattgattc tttcccttta
      721 taagaaataa aactcccacc gaagagtcca attagatttc cccttagaat ccacatcata
      781 cccaaggtag caattagagc aggtatctta aatttttgag ttattaaccc gttaaaataa
      841 ccgatcatgc atccaaaagc aatcgcaata attaaaccga ccggataagg aaaaccataa
      901 ttagataaaa gagtggaaat aactgccgcc caaccataaa ccgatgctat agagagatca
      961 atttctcctg aaataataac gaaagtttga cccaaggcta atattcccaa ttctgcaccg
     1021 atcaggagta ttccaggaag gataagcggc ccaagaaaat ttggagataa tattgaaaaa
     1081 aatacaaaaa taatcgctaa ggcaaggata acggttattt cgtaacgtaa ggtgaatttt
     1141 ctcttaaatg gtatatcctg aatagctact tgattgtcct gataatgatt aggtatgttt
     1201 ttcacgctct cctcctacgt tctctccttg tccttttaac cctcaagtct taagttttta
     1261 ggagtttttt tatcaaggcg ctattgatta ctgcagcaat catgagcaag attccaacca
     1321 gaacattata atattcgact cgaattccat aggttgcaat tcctacttgt actaccttaa
     1381 gaaggaaggc acatatcgat gttccaatga tactcccaat tccgccggta agaacagtcc
     1441 ctccaataac agcagctgct actgcctcta attccattcc tgtagctaca ataggatcaa
     1501 ggtaactaga acgaaccaaa aacgatgctc cagcaaatgc ggctaaagat gatgaaatca
     1561 taaaacagat cattttggtg cgaaatatat taacccctaa agcccgagcg gtttgaatac
     1621 ttcctccggt tgccataatc cagttgccaa acttggttcg attgaggaca atgctgaata
     1681 caactgctaa aatacagaac cagataaatt gattcggtat gaatccgatt tgtccaccta
     1741 ataccttaaa taatcgagta ggaactccct cataataagt aggttctcca ttggttaatc
     1801 cagctaaaac tgccctccaa aaaatcataa atcccagggt aacaacaaat gacggaattt
     1861 taaatttcat agtgaaaaat ccatttaaga atccagcacc aattccaaat attattccaa
     1921 gaataaaact gaccagaatc ggcaaaccaa attgattact aattaaaact atgtaattac
     1981 tgaaaacaaa aaccgatgca actgaaagat ccatttcacc ggagataatt aataaggtta
     2041 cacctaaggc aataattcct aattcggaac cggttaaaaa aatgacattt agcatgcttg
     2101 aggaaaaaaa acctgctgat atccgagaaa aaagaataaa aaaagtcatg aggagaataa
     2161 caactattgt ttccgatcgc attagaatgg attttgctga ttttggaggt ttcagatgag
     2221 tttgctcagg catcttttct cccccttctg ataatttcca ttataatttc tggagag
//
LOCUS       DAOSOL010000236         2263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_47796_length_2263_cov_2.911232, whole genome shotgun sequence.
ACCESSION   DAOSOL010000236 DAOSOL010000000
VERSION     DAOSOL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47796_length_2263_cov_2.911232"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PK171_05685"
     CDS             complement(<1..385)
                     /locus_tag="PK171_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOT05713.1"
                     /translation="MRQFAVFGLGIFGKNIALALYQQGFMVLGVDIDEAPVRDLASKI
                     SEVVQADTTDEKVLEALGIRNFDVAIVSIGNDIQSSVLTTLALKDMGVPFIVARAINE
                     NHGKILSKVGADRVIFPEKDMAIKVA"
     gene            complement(424..852)
                     /locus_tag="PK171_05690"
     CDS             complement(424..852)
                     /locus_tag="PK171_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HOT05714.1"
                     /translation="MKSNTLRVGLTGVASAIVGKENTADKFEKEMVPAFATPMLVSLM
                     DNAAFYAVKNHLSDGYESVGTRISISHIAATPPGMKVTARATLVEIYRNRLVFEAEAF
                     DEIEKIGEGKLERFVVKRENFLKKLEQKTRMKSKSLDERQ"
     gene            complement(849..>2263)
                     /gene="ftsH"
                     /locus_tag="PK171_05695"
     CDS             complement(849..>2263)
                     /gene="ftsH"
                     /locus_tag="PK171_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT05715.1"
                     /translation="ELSEVVDFLKNPRKFQKLGARIPRGVLLYGPPGTGKTLLAKAVA
                     GEAGVPFFSISGSDFVEMFVGVGAARVRDLFGNAKRNAPCIIFVDELDAVGRHRGAGL
                     GGGHDEREQTLNQLLVEMDGFDPNQGVILIAATNRPDILDPALLRPGRFDRRIAVPRP
                     DVLGREAILKVHTKGKPLGKDIDLKILARRTPGFVGADLANMMNEAALLAARKGKDVI
                     EMSDMEESIDKVIAGPERKNVIISDKEKKVVAYHESGHALVARFLPNTDPVHKISIIP
                     RGGAALGYTLQLPTEDRYLISKQELMTRLAVLLGGRVAEEIVFEDITTGAHDDLQKAT
                     EIAREMVCEYGMSDTLGPLTLGRRHKEVFLGKDIAEDRNYSEEVAFTIDKEVRSIIDS
                     AYEQARKLIIDNRDKLDKLTAILLEKEVLEREEIDKILDINGNEIPEFSSEEDKNKQA
                     AAEQLLSDFPSNQEGRINTK"
BASE COUNT          633 a          551 c          358 g          721 t
ORIGIN      
        1 tggcaacttt tattgccata tctttttccg gaaatattac ccgatcggca ccaactttac
       61 ttagaatttt tccgtgattt tcattaattg ctcgagctac gataaatgga actcccatat
      121 cctttaaagc taaagttgtg agaacactcg attgtatatc atttccaatg ctcacaatag
      181 ctacatcaaa attccttatc ccaagcgcct ctaaaacctt ttcatcagtg gtatcggctt
      241 gaacaacttc actaatctta cttgccaggt ctctcactgg ggcttcatca atatccaccc
      301 ctaaaaccat aaaaccttgc tgatagaggg ctaacgctat attcttacca aaaataccta
      361 aaccaaatac agcaaattgt cgcatttact catcaccatt tcacatgaaa caacatttat
      421 acattattgt ctttcatcta aagattttga tttcatccgg gttttttgtt ctagcttttt
      481 caaaaaattc tctcttttaa ctacaaatcg ctctaatttt ccttcaccaa ttttttcgat
      541 ttcatcaaaa gcttcggctt caaaaactaa tcggtttcta taaatttcaa ccaaagttgc
      601 ccgagcagtt actttcatac ctggtggagt cgcagcaata tgagaaatac tgatccgggt
      661 cccaacactt tcataaccat cagaaagatg attcttgact gcataaaaag cagcgttatc
      721 catgagcgat accaacatag gagttgcaaa tgccggcacc atttcttttt cgaacttatc
      781 ggcggtattt tctttaccaa caatagctga ggcaaccccg gttaacccaa cccgaagggt
      841 attactcttc attttgtatt aatcctccct tcctggttcg aaggaaaatc tgataataat
      901 tgttcagctg cagcttgttt atttttatct tcttctgacg agaattcagg aatttcattc
      961 ccgtttatat ctaaaatttt atcaatttct tctctctcta agacttcctt ttccaacaaa
     1021 attgctgtga gtttatctag tttatcacga ttatcaatga ttagtttccg agcttgttca
     1081 taagcactgt caataatgct tctgacctct ttatcgatgg taaaggcaac ttcttcactg
     1141 taatttcggt cttcagcaat atccttccct aaaaatactt ccttatgccg acgtcctaag
     1201 gttaaaggtc ccagggtgtc gctcatacca tattcacaaa ccatctctcg ggctatctca
     1261 gttgcttttt gtaaatcatc atgagcacca gtggttatat cctcaaaaac gatttcttcg
     1321 gctactcgcc cacccaaaag aactgctaaa cgagtcatta attcctgctt gctaataaga
     1381 taccgatctt cggtgggaag ttgtaaagta taacctaagg ctgcaccacc tcgcggaatt
     1441 atggatattt tatgtactgg atctgtattg ggaagaaatc ttgcgactaa ggcatgacca
     1501 gactcatgat aggctacaac tttcttttcc ttatcgctaa tgatgacatt tttcctttct
     1561 ggtccagcta tgaccttatc aatggattct tccatatccg acatttctat tacatctttc
     1621 cccttgcggg ctgcaagaag cgctgcttcg ttcatcatgt tggcaaggtc agctccaaca
     1681 aatcctggag ttcttcgagc caaaatctta agatcaatat ccttccctaa gggttttcct
     1741 ttggtatgca cttttaggat agcttctctt cctaatacat caggtcttgg tacggcgatc
     1801 cttctatcga aacggccggg tctgagaaga gccggatcga gaatgtcagg tcggttagtg
     1861 gcagcaatca gtataactcc ttgatttggg tcaaagccat ccatttcaac caaaagctga
     1921 ttcaatgttt gttccctttc atcgtgtcct ccccctaaac cagcaccccg atgtcgtcct
     1981 acagcatcca gttcatcaac aaaaattata caaggagcgt tccgtttggc atttccaaac
     2041 aaatctcgaa cccgtgctgc accaactcca acaaacattt caacaaaatc agaaccactg
     2101 atactaaaaa aaggaactcc tgcttcacca gcaactgcct tggccaaaag agtctttcct
     2161 gtacctggtg gtccgtataa caatacacca cgggggattc gagctcctaa tttctgaaat
     2221 ttacgcggat tttttagaaa atcaaccact tcacttaatt ctt
//
LOCUS       DAOSOL010000237         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_47997_length_2256_cov_3.130395, whole genome shotgun sequence.
ACCESSION   DAOSOL010000237 DAOSOL010000000
VERSION     DAOSOL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47997_length_2256_cov_3.130395"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PK171_05700"
     CDS             complement(<1..444)
                     /locus_tag="PK171_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05716.1"
                     /translation="MDKDRYILRLKGVSKFFPGLKALDQVDFDLRYGEIHALVGENGA
                     GKSTLIKIVTGVHQPDEGEIVYQDQRVIFSNPMVANRYQIAAVYQQPASFPHLTVTEN
                     IFLGHPLIQPQSRRLLWKEMHQQAKALLSSLGAEVSPTAVMGSLSV"
     gene            complement(530..1537)
                     /gene="rhaS"
                     /locus_tag="PK171_05705"
     CDS             complement(530..1537)
                     /gene="rhaS"
                     /locus_tag="PK171_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT05717.1"
                     /translation="MKKLFTLLLVISVGIFLMSANAFSEGHTVAMVVKNLGNPFMDAC
                     GKGAEEALNELGDTLIFQGPATPTVEGQIEIIENLIAQKVDAICVTANDFDALVPVLK
                     KAMDNGIKVVSFDSAVNAGGRIVHVNQADSEQIGKLQVQAVAEMINFEGEIAILSAGA
                     TMTNQNTWIEFMKKELEDPKYDKMELVTIVYGDDLRDKSYNEAMGLFKSYPNLKGIIS
                     PTTVGISATAKAIFDAGLVGKIQLTGLGLPSEMAEWIHNDTCTAMFLWNPIDLGYLAA
                     NVAGLLCHGDITGSEGEKFTGGKMGEYTIVKAADGGTEVLLGPPFRFDKSNIDDWKDV
                     Y"
     gene            1854..1994
                     /locus_tag="PK171_05710"
     CDS             1854..1994
                     /locus_tag="PK171_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05718.1"
                     /translation="MRRENLLLHLRHCEESNRSLVGRRGNLIRLLSHSEERLVRRENP
                     IF"
BASE COUNT          643 a          490 c          415 g          708 t
ORIGIN      
        1 tacactcaaa gaacccatca ctgcggttgg gcttacctca gcccctaatg aggatagcag
       61 tgcttttgcc tgttgatgca tttctttcca gagtaatctc ctgctctgcg gttgaatgag
      121 gggatgacct aaaaaaatat tttcggtaac agtaagatga ggaaaggaag ctggttgttg
      181 atagacagcc gcaatttgat agcggttggc gaccatggga ttagaaaaaa tgactcgctg
      241 atcttgataa acaatctctc cttcatctgg ttggtgaact cccgttacta ttttaatgag
      301 agttgatttt ccagctccat tttcaccaac cagggcatgg atttctccgt atcggagatc
      361 aaaatcaacc tggtctaatg ctttcaaacc ggggaaaaat ttcgagactc cttttaacct
      421 taaaatataa cgatccttat ccatatcgcc attcccatta ataagctcaa ctttaggaat
      481 atattataaa agggcaggaa tgaacttcct gccctttttt cgtgcttctt taatagacat
      541 ctttccaatc atcaatatta cttttatcaa agcggaaagg aggtccaagc aatacttcag
      601 ttcccccatc ggcagctttt acaatagtat attctcccat tttcccacca gtaaattttt
      661 ctccttcact tccggttata tcaccatggc atagcaggcc agccacatta gcagccaggt
      721 aacccaagtc aattggattc cacaagaaca ttgcagtaca ggtatcgttg tgaatccatt
      781 ccgccatttc actgggaagc ccgagtccgg tcaattgtat tttccccacc agcccagcat
      841 caaatatagc tttggcggtt gcgctgattc ctactgtagt aggtgaaata attcctttga
      901 gattgggata agatttaaac agacccatcg cttcattgta gcttttatct cgcaggtcat
      961 ctccgtaaac aatggtaacc agttccattt tgtcgtactt gggatcttcc agttcttttt
     1021 tcataaactc gatccaggtg ttttgattgg tcatggttgc tccagctgag agaatcgcaa
     1081 tttctccttc aaaattaatc atttccgcga cagcttgtac ttgcaatttt ccaatttgct
     1141 cagaatctgc ctgattgaca tgcacaatac gtcctccagc attcactgcc gagtcaaatg
     1201 ataccacttt aataccatta tccatggctt ttttaagcac cggaaccaaa gcatcaaagt
     1261 cattggcagt tacacaaata gcatcgactt tttgggcaat caggttttct atgatctcga
     1321 tttgaccttc tactgtgggg gtcgctggtc cttggaaaat cagagtatcg cctaattcat
     1381 tcagggcttc ttctgctcct ttgccgcagg catccataaa tggattacct aagtttttca
     1441 caaccatggc aacggtatga ccttcggaga aggcatttgc agacataaga aatattccca
     1501 ccgaaataac caaaagaagt gtgaaaagct tcttcatctt tctttctacc tccttataaa
     1561 acttacaatt tcattaaact ttgcctacac ctttgaaacc tactaaatga tcaacaaata
     1621 taagaaaatt cttcacctcc ctttaccgat ttaaaaaata tttttctaac tattatttta
     1681 tgtatccact ttgcttcctg tcaaggcaat attaaatttt taaccagaga ggatttattt
     1741 tttgaaaaaa taatttttag atgagctttt ttgtggtcga ccccatgatt cgctgtagtt
     1801 ccagatgaag acgtaaagat cagagaggtg agggggcgag ggaggagcat atcgtgcgac
     1861 gtgagaatct cctccttcat ctccgtcatt gcgaggaatc caaccgttct ttggttgggc
     1921 gacgtggcaa tctaattcgg cttctcagtc attctgagga gcgtcttgtg cgacgtgaga
     1981 atcccatttt ttaaagaatt ttataaaaaa caaagaaata taaaagatga gatcctcacg
     2041 gcttctgaat acgaagcctc agattgacgg ctgtggggtt agttagagat cttcacatcc
     2101 tcaccaagca agggttcaag atgacgacat tttttacttt ttacaatatt attggacaga
     2161 ataatacttc acatttttgg gtttataatt tttagtaatt ctttaaaaaa acatctcaca
     2221 tcataaatta atggagatga gttagctatg gatcat
//
LOCUS       DAOSOL010000238         2241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_48391_length_2241_cov_4.161940, whole genome shotgun sequence.
ACCESSION   DAOSOL010000238 DAOSOL010000000
VERSION     DAOSOL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48391_length_2241_cov_4.161940"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..1726
                     /locus_tag="PK171_05715"
     CDS             230..1726
                     /locus_tag="PK171_05715"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05719.1"
                     /translation="MDENQPLLNIVNVSKSFPGVRALNKVNMTLISGEVHALVGENGA
                     GKSTLVKIISGAYAPDEGSIDIGDKSFRSLNPYQARLNGIATVYQEQQLVPWLTVAEN
                     IFLGMEPRTRWGSIDFPTMKQEAEERIRSYGLSIQPTELVAALSVAARQEVAILKVLA
                     RNKAKVLLLDEPTASLTGSQVDFLFSLIHQLQQQGIGILYISHYLDEVLEISQRITVL
                     RNGQGVGTFQKGDLNKETLIEKMAGHKLDFSTVIKKPVGEKILELKEISCYNILQNVN
                     LSLHNEEILGILGGNDSGCHELIRLLAGLIPFSQGKMFLRGEPYSPTSIYDALEKGLF
                     FIPENMRKEGLVLPLSLAKNITLSNLKEVLTQGFIHLKKEGNQAQNLVKALKIVTPSV
                     KTEVQYLSGGNQRKVLFAKALFADAQIWLLEEPTQGVDVEARQEIHRLLFDIKSKQKS
                     ILLVSSDLEELITVADRIIILRRGQIVVEIADSSSTSPKELLQAMLGG"
     gene            1729..>2241
                     /locus_tag="PK171_05720"
     CDS             1729..>2241
                     /locus_tag="PK171_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05720.1"
                     /translation="MFSFATIGRNILVLVLLAIIIFTGIINPRFLSLQNILNILQQIS
                     VLGIVSIGVTILLISGGLDLSVGSLISLSAYVGGLTIVQTHSLGLGILALFACAVFFG
                     FLNGVLAATERAHPFIITLGTMTFLQGIAVVISRGSPINGMGGLYELIGIRDIFGLPA
                     PSIIFIGLLIL"
BASE COUNT          652 a          419 c          472 g          698 t
ORIGIN      
        1 agaaaaagat gagatcctca cgcggaaaag caccgctcag gatgacgtcg gcggcgtcgg
       61 atgagatcct cacggcttca aaaaacgagg gctcaggatg acggattaaa ggcacacccc
      121 cctaaccccc ctcaatgggg gaattcattt gatggtattt tcaggataga ccatacctta
      181 tgatggtggt aattgatttg gagtttaata aatcaggagg ttttatcttt tggatgaaaa
      241 tcagcccctc ttaaatattg tgaatgtttc gaagagtttt ccaggagttc gagccttaaa
      301 taaagtgaat atgactttaa tatctgggga agtacatgct ctggtcggag aaaatggagc
      361 cggtaaatcc actttagtga aaattatatc cggagcctat gcccccgacg aaggatcgat
      421 cgacataggg gacaagtctt ttcgatcttt gaatccttat caagccagat taaacggaat
      481 tgctaccgtt tatcaggaac aacaattggt tccctggttg accgtagcag agaatatatt
      541 tttagggatg gaacctcgca cccgttgggg aagtatagac tttcctacca tgaaacaaga
      601 ggctgaggag aggattcgct cttatggctt aagtattcaa cctaccgagt tagttgctgc
      661 tttatccgta gcagcccgcc aggaggttgc catcttaaaa gtactagctc gtaataaagc
      721 taaagttctt cttcttgatg aaccaaccgc atctttaacc ggatctcaag tggatttttt
      781 gtttagcttg attcatcaac ttcaacagca agggatagga atactctaca tttcccacta
      841 tttagatgaa gttttagaaa tttctcaaag aataactgtg ttaagaaatg gacaaggagt
      901 tggtactttc caaaaagggg atctaaataa agaaacctta attgaaaaaa tggcggggca
      961 taagcttgat ttctctacag ttattaaaaa accggttggg gaaaaaattt tagagcttaa
     1021 agaaatttcc tgttataaca tccttcaaaa tgtaaacctc tcattacaca atgaggagat
     1081 actcgggatt cttggaggaa acgattcagg ttgccatgaa cttattcgtt tgcttgccgg
     1141 acttatacct ttttctcagg gaaaaatgtt tttaagagga gaaccttatt ctcctaccag
     1201 catttatgat gccttagaaa agggattatt ttttattccg gaaaatatgc gtaaggaagg
     1261 tttggtactc cccctttctt tagccaaaaa cattactctt tctaatctta aagaagtact
     1321 cactcaaggc tttatccacc taaaaaaaga gggaaatcaa gcacaaaatt tagtgaaagc
     1381 gctaaaaatt gttacaccat cagtgaaaac tgaagtacag tatttgagtg gagggaacca
     1441 gaggaaagtt cttttcgcca aggccctttt tgccgatgcc caaatttggc tcttggaaga
     1501 accaactcaa ggtgtagacg tagaagcccg tcaggaaatc catcgtttac ttttcgatat
     1561 caaatcaaaa caaaaaagta ttcttctcgt ctcttcggat cttgaggaac ttattaccgt
     1621 agccgatcga attatcattt tacggagagg gcagatcgta gtggaaatag ccgattcttc
     1681 ttctaccagc cccaaagaat tactgcaagc tatgttagga gggtaatcat gttttctttt
     1741 gccaccattg gtagaaatat attagtgtta gttcttttag ccattataat ttttactggt
     1801 ataatcaatc ctcgtttttt aagtctacaa aacatattga atattcttca acaaatctcg
     1861 gttttgggta ttgtcagcat tggggtgacc atactcctca tttctggggg gctggacctt
     1921 tcggtcggaa gtcttatttc tcttagtgct tatgtcggtg gtcttactat tgttcaaact
     1981 cattctttgg gtttgggtat tcttgccctc tttgcctgcg cagttttttt tggattttta
     2041 aatggtgttt tagcagcaac cgaaagggct caccctttca taattacttt aggaactatg
     2101 acttttcttc aaggtattgc agtagtgata agccggggaa gtccgattaa tggaatgggt
     2161 ggactttatg aattgattgg tattcgtgat atttttggat taccagctcc gtcaattata
     2221 tttattggct tactgattct t
//
LOCUS       DAOSOL010000239         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_49098_length_2218_cov_3.212205, whole genome shotgun sequence.
ACCESSION   DAOSOL010000239 DAOSOL010000000
VERSION     DAOSOL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49098_length_2218_cov_3.212205"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PK171_05725"
     CDS             <1..355
                     /locus_tag="PK171_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05721.1"
                     /translation="RITRSSMLEIIRQDFIRTERAKGLPERMVIFKHALKNALIPIIT
                     VIGMEFGLLLGGAILTETVFAWPGLGRYTVDAVYARDYPAIQGSVLFIAFIFVVVNLI
                     TDVLYAYINPRIRYH"
     gene            356..1231
                     /locus_tag="PK171_05730"
     CDS             356..1231
                     /locus_tag="PK171_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05722.1"
                     /translation="MMKNTSGVFSDFFYMIRKNKSAFVGLIVIVAMVIVALLAPIISP
                     NNPLQRSLANRLKPGFWAGEEYNQFPLGTDYLGRCLLSRIIWGARTSLSVGFIAVLIS
                     TVFGLILGIAGGYYGGWVDTFLMRIVDVLFAFPSILLSITIMAALGAGLEKAMIAIGI
                     VYIPQMARVVRSAILVIKEMDYIEAEKALGASHFRIIWHHILPNALAPVIVYSTLSVA
                     GAILDAAALGFLGLGALPPTPEWGSMLSNSRQFLTSGAWWAATFPGLAIMLSVLGLNL
                     LGDGLRDALDPRLRV"
     gene            1237..2202
                     /locus_tag="PK171_05735"
     CDS             1237..2202
                     /locus_tag="PK171_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05723.1"
                     /translation="MNDIILQINNLKTYLTTPSGIVQAVDGVDLNIAANQTLGLVGES
                     GCGKSMTALSILKLYPKPQGKIVDGQIIFLGDDLVKKTEEEMYTIRGKKISMIFQEPM
                     TSLDPVFPVGKEIVEVLTIHQKLSIPEAEKQAIELLKRVRIPEPKRRFREYPHQMSGG
                     MRQRVMIAMALACRPALLIADEPTTALDVTIQAQIISLINDLKREFQTAVLLITHDLG
                     IVAETCQQVAVMYAGKIVERADVYSIFSNTRHPYTVSLLNAIPRIQRDRQRLESIPGR
                     VPNLCFPPGGCRFHPRCSKKMTICSTTEPLLKEIEKDHWVACHLY"
BASE COUNT          596 a          473 c          466 g          683 t
ORIGIN      
        1 ccggattacc cgatcttcta tgcttgaaat tatccgccaa gactttattc gtaccgaacg
       61 agctaaaggt ctcccagaaa gaatggtaat cttcaaacat gctttaaaaa atgcactgat
      121 tcctatcatc acggttattg gcatggaatt tggtctcctt ttaggaggag ccatcttaac
      181 tgaaacggtt tttgcttggc cgggattggg tcgatacaca gttgatgcag tttatgctcg
      241 tgattatcca gccattcagg gatcggttct ttttattgcc ttcatattcg ttgtagtcaa
      301 tctcattact gatgtattgt atgcctatat taatccacgg atcaggtacc attaaatgat
      361 gaagaatact agtggagttt tttccgactt tttttatatg attcgcaaaa ataagtcagc
      421 ttttgtgggg ttgatcgtta ttgttgctat ggtaattgtt gccttattgg cacctatcat
      481 ttcccccaat aatccactgc agagaagcct cgccaatcga ttgaagcccg gattctgggc
      541 aggcgaagaa tataatcagt tccccttggg taccgattat ttagggcgtt gtttactgtc
      601 aagaatcatc tggggtgcac gaacatcttt atcagttggt tttattgccg ttcttatctc
      661 cactgttttt ggtcttattt taggtatagc tggtgggtat tatggaggat gggtagacac
      721 atttttaatg cgtatagttg atgttttatt tgcctttcca agcattcttt tatcgattac
      781 aattatggct gccttaggag caggattaga aaaagccatg atcgctattg gaatcgttta
      841 tattccccag atggcccgag tggttcgaag tgctatttta gtgatcaaag aaatggatta
      901 tattgaagct gaaaaagcct taggagcctc ccatttccgg attatttggc atcatatcct
      961 tcctaatgcc ctggctccgg ttattgtata ttccaccctg agtgttgccg gggctattct
     1021 cgatgctgca gctttaggct ttttagggct cggggctctt cctcccacac ccgaatgggg
     1081 atcgatgctt tccaatagtc gacaatttct aacttcagga gcctggtggg cagcaacctt
     1141 cccgggctta gcgattatgt tgtcggtatt agggttaaac ctcttaggag atggtctacg
     1201 cgatgctctt gatccgcgtt tacgagtatg aaaactatga atgatattat tctccaaatc
     1261 aataatttaa aaacttatct gaccactcca tcaggaattg tccaggctgt tgatggggtt
     1321 gaccttaata tagctgccaa ccaaaccctc gggttggttg gtgaatcagg ttgcgggaaa
     1381 agtatgactg ccctctccat tttaaaactc tatcccaaac cccagggaaa aattgtggat
     1441 ggtcaaatca tctttttagg cgatgatcta gttaaaaaaa ccgaagagga aatgtatacc
     1501 atccgaggga aaaaaatttc aatgattttt caagaaccaa tgacctccct ggatccggta
     1561 tttcctgtcg gaaaggaaat tgttgaagtg ctgactattc atcaaaaact tagtatacca
     1621 gaagcagaaa aacaagccat tgaacttcta aaaagagtgc gcatacccga accaaaaaga
     1681 cggttcagag aataccccca tcaaatgagt ggtgggatgc gacagagagt catgattgct
     1741 atggctttag cttgccgccc agctctttta atcgccgacg aaccaaccac tgctcttgat
     1801 gtgactatac aagctcaaat catctcactt attaacgatc tcaaaaggga atttcaaact
     1861 gcggtccttc tcatcaccca tgatttaggt attgtagctg aaacctgtca gcaagttgcg
     1921 gtcatgtatg ctggaaaaat cgttgaaaga gctgatgttt attccatctt ttcaaatacc
     1981 cgtcaccctt acaccgtttc tctattaaat gccattcccc gaattcagag ggatcggcaa
     2041 cgtttggaat caatcccagg aagagttccc aatctatgtt ttcccccggg tggatgccga
     2101 ttccatcccc gttgttctaa aaaaatgacg atttgctcaa ccaccgaacc attattaaaa
     2161 gaaattgaaa aggatcactg ggtcgcatgt cacctttatt gatatccctg caacagct
//
LOCUS       DAOSOL010000240         2192 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_49898_length_2192_cov_2.744502, whole genome shotgun sequence.
ACCESSION   DAOSOL010000240 DAOSOL010000000
VERSION     DAOSOL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49898_length_2192_cov_2.744502"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK171_05740"
     CDS             complement(<1..211)
                     /locus_tag="PK171_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HOT05724.1"
                     /translation="MPETISLSEIKKVVALAKLSIPDIDLEQFFLEINDILSHFDKLN
                     RLELKEVSPTSHISWSQPPSNPDEPR"
     gene            complement(331..>2192)
                     /gene="ligA"
                     /locus_tag="PK171_05745"
     CDS             complement(331..>2192)
                     /gene="ligA"
                     /locus_tag="PK171_05745"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT05725.1"
                     /translation="LTPDSPTQRVAGKPQDGFPSFLHSQPLLSLNNAVLEDDVREFDQ
                     RLQRLLKQNQVEYVVELKIDGLAINLRYEEGILTHGATRGDGTTGEDVTPNIRTIKTI
                     PLRLLGDKIPEVIEVQGEIFMGKNAFNRLNQERTAKSEPPFANPRNAAAGSLRQLDPR
                     VTATRELQLFAYGATLIESSYSPTTQWELLTYLKKLGFKVNPHIQLVSSIEEAIHIHR
                     KWESERKQLEYEIDGLVIKLNFLTDRSRLGTTSKSPRWAIAYKFEATTELTRVLDIEV
                     NVGRTGTLTPVALLEPVNIGGVIVKRATLHNEDEMKRKDVRIGDWVLVGRAGDVIPEV
                     VRVISEQRTGSEIIFQMPSTCPACHAPVKREVGEAAWKCVNLNCPAQRKEKIIHWASR
                     DAMDIEGLGEKLVNQLIETSLVQTLSDLYRLNIEELIDLERMGPKSAGNLIQAIDRSK
                     RKELARFIYALGIPFVGSHVAQILTNHFSSLEQIIEASEYQFLIIDGIGPKVSQSILN
                     FFSIPENLTLLKELKALGVHPIQKKKEKSVKPQEFFQEKTFLFTGTLSHFTRKEAEDL
                     VVSRGGKVAKAISSQVNYLIVGEQPGSKLQQAIKKNIPLLTESEFEEKIQSDF"
BASE COUNT          600 a          448 c          464 g          680 t
ORIGIN      
        1 ctcgaggctc gtcgggatta gatggtggtt ggctccagga gatgtgtgaa gtgggtgaaa
       61 cctccttgag ttcaagacga ttcaatttat caaaatgtga aaggatatca ttgatttcta
      121 agaaaaactg ttccaaatcg atatcgggaa tggacagctt ggcaagagca acgacttttt
      181 ttatttccga caatgaaatt gtctctggca atcgacccac tcctttcctg cggggggtat
      241 attctgttat caataactgt atttagttct ttgatgaatc tgatgttgtc atacaatacc
      301 atactattat agtgaaaagc tttcctcttt tcaaaaatcc gattggattt tctcctcgaa
      361 ctccgattca gttaaaagag gaatgttctt ttttatcgct tgttggagtt ttgaaccagg
      421 ttgttctccc actattagat aattaacctg ggaagaaatg gctttagcaa cttttcctcc
      481 tcgggaaacg accagatcct cagcttcttt tcgagtaaaa tgactgagag ttccggtaaa
      541 aagaaatgtt ttttcctgaa aaaattcttg gggttttacc gatttttcct tttttttctg
      601 gataggatgg acaccgaggg cttttaattc cttgagcaaa gtcagattct caggaataga
      661 aaagaaattg agtattgatt gagagacctt aggaccgatt ccatctatga ttagaaattg
      721 atactctgat gcttcgataa tctgttctaa agaagagaaa tgattggtga gaatctgggc
      781 aacatgggag ccgacaaaag gaattcccaa agcatagata aatcgagcta attcttttct
      841 ttttgaccga tcaatggctt gaatcagatt acctgctgac ttaggtccca ttcgctccaa
      901 atcaattaat tcctcgatat tcaagcgata gagatcggaa agggtttgaa ctaaactggt
      961 ctcaataagt tggttgacta atttttcccc cagcccttca atatccatag cgtcccgtga
     1021 agcccaatga ataattttct ccttccgctg agctggacaa tttaggttga cacatttcca
     1081 agcggcctct ccgacttctc tttttactgg agcatgacac gctggacagg ttgagggcat
     1141 ttgaaaaata atttccgaac cggttctttg ttctgaaata acccgaacca cttcggggat
     1201 gacatctccg gctcgaccaa ccaaaaccca atcgccaatc cggacatctt tcctcttcat
     1261 ttcgtcttca ttatgaagag tagctcgctt tactataact ccaccgatat taactggttc
     1321 taataaagca actggagtaa gtgttcccgt ccgccctacg ttaacttcaa tgtcgaggac
     1381 tctggttagc tcggttgttg cttcgaattt gtacgcaatt gcccaacggg gactcttgct
     1441 ggttgttcct aaacggctcc gatcggtaag gaaattgagt ttaatgacca atccatcgat
     1501 ttcatattct aattgctttc gctcactttc ccacttgcga tggatatgaa tggcttcttc
     1561 tatggaggat actaattgaa tgtggggatt aactttgaat cccaattttt ttaaataggt
     1621 tagaagttcc cattgggtgg taggtgaata gctgctttca atcaaagttg ctccataagc
     1681 aaacaattga agttcccggg tagctgtcac tcgtgggtca agctgacgaa gtgaaccagc
     1741 agcggcatta cgaggattgg caaagggagg ttcgctttta gcagttcttt cttggttgag
     1801 acggttaaaa gcgttttttc ccataaatat ttcaccctgg acttcaataa cctcgggaat
     1861 tttgtcaccc aataagcgaa gagggatggt tttaatggtc cgaatgttag gagttacatc
     1921 ttcaccggta gtaccatctc cccgggtagc tccatgagta agaatccctt cttcatacct
     1981 gagattgatt gccaaaccat caatttttaa ttcaaccaca tattcaactt ggttttgttt
     2041 cagaagtctt tggagccgtt gatcgaattc acgaacatca tcttctaaaa cagcattatt
     2101 aagactcagg agaggttgag aatgcaggaa agatgggaaa ccatcctgag gtttaccggc
     2161 aacccgttgt gtaggtgaat cgggagttag at
//
LOCUS       DAOSOL010000241         2189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_49973_length_2189_cov_4.893158, whole genome shotgun sequence.
ACCESSION   DAOSOL010000241 DAOSOL010000000
VERSION     DAOSOL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49973_length_2189_cov_4.893158"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PK171_05750"
     CDS             complement(<1..477)
                     /locus_tag="PK171_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOT05726.1"
                     /translation="MNIDALFSLNCGMYIISSGYNGKHNGMIINALVQVTAFPNQIIA
                     SVNKESLTLEYIEKSGLFNITVLDKDAPLQLIGLFGFQTGKKIDKFSKTQFISGKNGI
                     PIVTEHTVSYVETKVVSKLDAGTHVLFLGEVLESEFLTQEEPMTYNYYRDVKGGKVP"
     gene            987..2174
                     /locus_tag="PK171_05755"
     CDS             987..2174
                     /locus_tag="PK171_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT05727.1"
                     /translation="MFYNYFMVYLPKKITDIDQKVKILGESAQYDLCGACANQVERKR
                     NPQGGWIYPTVTPDGERVNLLKILLSNDCIHNCYYCGNREGRKGDRVRFSPEELVRIF
                     LNMKDRGLVKGLFLSSAVDRNPFQTMDDMLKVTELLRLKYHFRGYIHLKIFPESTDDY
                     IETAIRLATRVSINIEAPGSDYLKSIAPQKNFNRIWNRFLYLKKLSQKGVRPRAGFTT
                     QLVIGGSEEKDLDIMRTVSTLYRDFSMTRAYYSAFHPISGTPLAEKQPESTWREHRLY
                     QADFLFRKYHFSLDELILNQKGNLPLEIDPKTLWALNHPEVFPLEINTASYQELLRIP
                     GIGPISVKRIIQYRQKTPFRDLSSLQSLGIRVQAATPYILLSGKQFRIPQQLSLFSEY
                     IAG"
BASE COUNT          687 a          408 c          401 g          693 t
ORIGIN      
        1 aggaaccttc ccacccttga catcacgata ataattgtag gtcatgggtt cctcttgagt
       61 taaaaattcc gattcaagta cttctcccaa aaagagaaca tgggtaccgg catctaattt
      121 cgaaacaact ttggtttcga cataagaaac ggtgtgttca gtaacaatag ggattccatt
      181 tttcccagaa ataaattgag ttttagagaa tttatcaatt ttcttcccgg tttggaatcc
      241 gaaaagacca attaactgta aaggggcatc tttatctaaa actgttatat taaaaagacc
      301 acttttttcg atgtattcaa gagttaagct ttctttattg acgctggcaa taatctgatt
      361 agggaaagcc gttacctgga ctaatgcatt aataatcatt ccattgtgtt tcccattata
      421 accagaactg attatataca ttccacaatt taaagagaac agagcgtcta tgttcaaaat
      481 cgacaccacc ttttatccat ggaggaaatt aggagaattc attccctatt acgtttcata
      541 aattttactc gataaatggt ttgctgccaa atttatcttt tttcccttac cctttgggct
      601 aagggagatg gttcgggatt cgatttttac cagttctttc aaatacccct tgcacccttt
      661 gttaaaaagg ggaaactgat tcttactcgg gcaatactgt gaacaatcaa aatattctaa
      721 tatttaacct gatttgaaaa acgaggggaa tgataaatca agcccctaca aaagaatata
      781 aaatgtaggg gcataatgta ttgtgcccat tctttaatta atgtagtgac atgccatggc
      841 atgtcgaatc tttggttttc atacttatct aatgctaccg attggcatgg ttgtctgtct
      901 tatataaata ttagaagtat atcactctgt ttatattcat gccagctgtc ataaaattta
      961 aatgaaatat tccaaaatgg gtaaaattgt tttataatta ttttatggtt taccttccta
     1021 aaaaaataac tgatatcgat caaaaagtaa aaattctggg agagtcggct caatatgatt
     1081 tatgtggagc ctgtgcgaac caggttgaga gaaaaagaaa tcctcagggt ggatggattt
     1141 atcctacagt gacacccgat ggcgaaagag taaatctctt aaaaattctc ctttccaacg
     1201 actgtattca taactgctat tattgtggta atcgtgaagg acgaaaagga gatcgagttc
     1261 gtttttcgcc ggaagagttg gttcggatat tccttaacat gaaagataga ggtttagtga
     1321 aagggctatt tttaagttca gcagttgata gaaatccttt ccaaaccatg gatgatatgt
     1381 taaaagttac cgaactgctg aggttaaaat atcattttcg aggatatatt catctaaaaa
     1441 tctttcctga gtcaaccgat gactatatcg agactgctat aagattggct actcgagtgt
     1501 cgatcaacat tgaagctcct ggatctgatt atttaaagag tattgctcct caaaagaatt
     1561 ttaatcgaat atggaataga tttctttatt tgaaaaagct ttctcaaaag ggggtaagac
     1621 ctcgcgctgg tttcactacc cagttggtta tcggaggaag tgaagaaaaa gatctggata
     1681 ttatgaggac agttagcact ctttaccgcg atttttccat gaccagagct tactacagtg
     1741 catttcatcc aattagcggg actcctctgg ctgaaaaaca accagagtcg acttggagag
     1801 agcaccgcct atatcaagcc gattttcttt ttcggaaata tcatttttct ctcgatgaac
     1861 tcatattgaa ccaaaaagga aatctcccct tggaaataga cccaaaaact ttatgggcat
     1921 taaatcaccc ggaggttttc cctttagaga taaatactgc ttcctatcag gagttactca
     1981 gaataccagg cattggtcca atatcagtaa aaagaattat tcaatatcgc caaaaaactc
     2041 cttttcgtga cttatcctca cttcaaagct tagggattcg tgttcaggca gctacacctt
     2101 atattcttct ttccggaaaa caattccgga ttcctcaaca gctttcactc ttttcagaat
     2161 atattgcggg gtaagctctc gtgccgctt
//
LOCUS       DAOSOL010000242         2181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_50229_length_2181_cov_3.080903, whole genome shotgun sequence.
ACCESSION   DAOSOL010000242 DAOSOL010000000
VERSION     DAOSOL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50229_length_2181_cov_3.080903"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            758..1978
                     /locus_tag="PK171_05760"
     CDS             758..1978
                     /locus_tag="PK171_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOT05728.1"
                     /translation="MPAVELGNKIHWIGVNDHTTDLFEGLWPITQEGVSYNSYLINDQ
                     KKVIIDLAKSIKVDEFYKRIAEIVDPSQVNYIILNHMEPDHTGVLRVLRKIAPQARIL
                     CSPKAKDMIQAFYGINDQVQVVQDGEEVQLGELTLQFFMTPFVHWPETMMTYEKKNRI
                     LFSCDAFGSYGALRGAIFDDECTDLEFYQQQALRYYANIVAKFSAQVLKAIEKLAGIP
                     VEIIAPSHGLIWRKNPSIILDLYKKWAEYAKIPGEPGVTLLYATMYGNTETLMDSVAQ
                     GVSQTGSSLEIFDVSRIHTSYILASLWSKSGVIIGAPTYENELFPSMAHVLDIAIRKG
                     VQHKKAAYFGSYGWAGGALRELEKQLALLKWQLTDTLVFQGGPSQEVLVQGKEFGRKF
                     AEMVLSGMNSEENG"
BASE COUNT          674 a          410 c          445 g          652 t
ORIGIN      
        1 tcagcctcgt ccctataaaa atcccttggc acctaaagaa gctttaactg agataaataa
       61 ccaatcaggg agacagtttg atccagaggt cgtcaaaagc ttccttaaaa taatagagga
      121 aaaagtaggt taaatcccca tgccctttat gatgcttaat gaggatgaaa atgcctattc
      181 agaaatgaat ctcccccttt agcaaagggg gtaaggggga tttgaatttt ttcctgctcc
      241 gtaattgcga ggagcatcct atgcgacgtg gcaatctctt ttccccagtc tgtcatcctg
      301 aggcttcgta ttcagaagcc gtgaggatct catcttttat tcgaatttct taattttttt
      361 ctcattataa ccaggaaaaa aacaccctcc ccgccgaaaa acggcacccc tccaaggagg
      421 ggaattgttg aatttattcc cccattgagg ggggattaag gggggtgtgc ctttttcttt
      481 tttgttttga tcgtgattta cacgttctgg tatttataag atagtaaaga aaacattaaa
      541 aatatcaaag aatgatcaat gatcgaatat cattatgaaa acagcaatga gaataagagc
      601 ggtgaacttg taaattgtga aaattttccc accttttctc tatctttcag ttttccttaa
      661 taagtccttc gtaagcaaaa tcaccatcaa acaaacaaat tcaggtataa tagagattga
      721 atttttattt ccgtaatgag aaggaggatg agaacctatg ccggctgtgg agttaggaaa
      781 caagatacac tggatcggtg tcaatgacca caccaccgac ctctttgagg gtttatggcc
      841 gattacccag gaaggagttt cttataattc gtatctcatc aatgatcaaa aaaaagtcat
      901 aatcgattta gccaaatcaa taaaagtcga tgaattttat aagcgaattg cagaaattgt
      961 cgatccttct caagttaatt atatcattct caaccatatg gaaccggacc acaccggtgt
     1021 tttgagggtc cttcggaaaa ttgctcccca agcaagaatt ctttgttctc caaaagccaa
     1081 agacatgatt caagcctttt atggaattaa tgatcaggtt caggttgtcc aagatggaga
     1141 agaggtgcaa ttgggtgaac taaccctcca attttttatg acaccttttg ttcattggcc
     1201 agagaccatg atgacctatg aaaagaaaaa ccgaatatta ttttcttgtg atgcttttgg
     1261 cagttatgga gcacttcggg gcgccatatt tgatgatgaa tgtaccgact tagaatttta
     1321 tcagcaacaa gccttgcggt attatgccaa tattgttgca aaatttagcg ctcaggtttt
     1381 aaaagcaatt gaaaaattag ctggtatacc ggtagaaatc atagcacctt ctcatggttt
     1441 aatctggaga aagaatccat cgatcatcct cgatctgtat aaaaaatggg cggaatatgc
     1501 aaaaatacct ggagaaccag gagtaaccct tctttatgcg accatgtatg gaaacaccga
     1561 aaccttaatg gattctgttg cccaaggtgt atcccagacc ggttccagcc ttgaaatatt
     1621 tgatgtttct cgcattcata ccagttatat acttgcctca ctctggtcaa aaagcggggt
     1681 tattattggg gctccaactt atgagaatga gctctttcca tcgatggccc atgttcttga
     1741 tatagccata agaaaagggg ttcagcataa aaaagccgcc tattttggaa gttatggttg
     1801 ggcgggagga gctctacgag aactggaaaa acaactggct ttgttaaaat ggcagttgac
     1861 cgataccttg gtatttcaag gtggtccttc tcaagaggtt cttgtccaag gaaaagaatt
     1921 cggaagaaaa ttcgctgaaa tggtcttatc gggaatgaat tctgaagaaa atggttaatt
     1981 aataagatgt agtcttttta gtctaaattc aattatttag ctgtgttgta atcattaaaa
     2041 ttaggaattc atgacaccat tacacaagat gaggatgaaa atacctattc agaaatcaat
     2101 ttcccccttt agctaagggg gtgaggggga tttgtgtttt ttactgcccc gtcattcaga
     2161 ctgtgtcaca aatccttttt t
//
LOCUS       DAOSOL010000243         2175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_50424_length_2175_cov_3.641981, whole genome shotgun sequence.
ACCESSION   DAOSOL010000243 DAOSOL010000000
VERSION     DAOSOL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50424_length_2175_cov_3.641981"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PK171_05765"
     CDS             <1..301
                     /locus_tag="PK171_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOT05729.1"
                     /translation="SGGNQQKVVLARELSRDVKLAIASQPTRGLDIAASEYVRNKLID
                     LKNNGSGVLLVSADLDEIRSISDRVIVLYEGQIIGELAPDADEYQYGLLMGGTAC"
     gene            295..1341
                     /locus_tag="PK171_05770"
     CDS             295..1341
                     /locus_tag="PK171_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05730.1"
                     /translation="MLKKTLNYLDRSSSILPELIAIALAFLISAIILLITGYQPIRAF
                     AALFRGAFGSKFAVGQTLTQATPILFTSLSFLLAFRSGIFNIGIEGQFLIGAFAAAVV
                     GSSITGLPPIIHVIISLVIGGIFGALWGLIPALLKIFTGASEVITTMMMSYIASHLTS
                     YLVNYRFKAPGWVAQTNPVQPSAILVKILPPTQLSVSFLISLGFSLLVFYFLFKTYLG
                     YQVCAVGLNPTAAENRGISVHSRILMAFLFSAFVAGVGGAGEVLGVHGRFVEGFSPGY
                     GWDGIAVSLVGRLHPFGCIIASILFGMLRAGGMSMTRSTKVPLDISLIIQSLVIILVA
                     APSFIKFVILRRKN"
     gene            1343..>2175
                     /locus_tag="PK171_05775"
     CDS             1343..>2175
                     /locus_tag="PK171_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05731.1"
                     /translation="MMNIVALINISFFQAALRMATPIAFASLGGMYNERAGVVNIGLE
                     GIMLNSAFFAVYATYLTGSSFMGIIAGVASGLVLGAILAVLTVKYHGNQIVVGTGFNV
                     FGLSFSAYMCQKIWGSRGASETIIGLEEKSIPILAKIPWIGPILNNQNVLVYLMILIF
                     ILSHILLYKTHWGLRLRAVGENPQAADTSGVNVYQYKYFYIILGCMVASLGGVFLSIG
                     HLNFFAWGMTSGRGFIALAAMILGKWTPFGCLAASFLFGITDALQMRLQALGILPSQI
                     IL"
BASE COUNT          523 a          423 c          480 g          749 t
ORIGIN      
        1 aagcggtggt aatcaacaaa aggttgtttt ggcgagagaa ctctcccgtg atgtaaaact
       61 ggctattgct tcccaaccaa ctcgaggatt ggatattgcc gccagtgagt acgtgcggaa
      121 taaattaatt gatctcaaga ataatggctc aggcgttctt ttagtttcag ctgatcttga
      181 tgaaatccgt tcgatcagtg accgagttat cgttctttac gaggggcaaa taattggaga
      241 attagctccc gatgccgatg aatatcaata tggtctgctt atgggaggaa ctgcgtgttg
      301 aaaaaaactt taaattacct cgatcgttcc agttctatcc ttcctgaact catagcgatt
      361 gcactggctt ttctgattag cgccatcatt cttctcataa ctggctatca acccatacgg
      421 gcttttgccg ctctttttcg cggagctttt ggtagtaaat ttgcagttgg acagacactc
      481 acccaagcta ccccgatact ttttacttca ttatcttttc tcttggcttt tcgttcagga
      541 atctttaata ttggaattga agggcaattt ttaatcggtg cttttgcggc agcggttgtt
      601 ggatcgagca taaccggtct tccaccgata atccatgtga ttatttcttt agttattggt
      661 gggattttcg gtgcactttg gggactcatc ccagcacttt taaaaatatt taccggtgcc
      721 agtgaagtta ttacaaccat gatgatgagt tacatcgcca gtcaccttac cagctatttg
      781 gtaaattacc gttttaaagc tcctggttgg gttgctcaaa ccaatcctgt tcagcccagt
      841 gcaattttgg ttaaaatcct accacccacc cagctttcag tttcttttct catttcttta
      901 ggtttcagct tgctggtttt ttattttctt tttaaaacct atctgggata tcaagtttgt
      961 gctgttggct taaatccaac agcagcagaa aaccggggaa tttctgttca ttcacgaatt
     1021 cttatggcat ttttattcag tgcttttgtt gctggggtgg gaggagccgg cgaggttctt
     1081 ggagtccacg gacggtttgt ggaaggattt tcacctgggt atgggtggga tggaatcgca
     1141 gttagcctcg tgggacggct ccaccccttt ggatgtatca ttgcatcgat tttattcgga
     1201 atgctacgtg ccggtggcat gtctatgact cgatctacca aagttccatt ggatatatct
     1261 ttgattattc aatccttggt gattatttta gttgctgctc catcatttat taaatttgta
     1321 attttacggc ggaaaaacta agatgatgaa tattgtagct cttatcaata ttagcttttt
     1381 ccaagcagca ctaagaatgg caacacctat tgcttttgct tctctgggtg gcatgtataa
     1441 cgaaagagct ggtgtcgtta acattggatt agagggaatc atgctcaatt cagccttttt
     1501 cgcagtatac gccacttatt taaccggaag ctcttttatg gggatcattg ccggagtggc
     1561 ttctggtttg gttttgggag caatattggc agttttgacg gtcaaatacc atggaaatca
     1621 aattgtagta gggaccggct ttaatgtttt cggacttagt tttagcgcct atatgtgtca
     1681 gaaaatttgg ggatcaaggg gtgcatcgga aacgattatt ggtttggaag aaaaaagtat
     1741 tcccattttg gccaaaatcc cctggattgg tcctatcttg aacaaccaaa atgttcttgt
     1801 ttatctcatg attttaattt ttatactttc tcacattctg ctttataaaa ctcactgggg
     1861 tcttcgactc cgagcggttg gagaaaatcc tcaagcagca gatacttctg gagtcaatgt
     1921 gtatcaatat aaatatttct acattatttt aggatgtatg gtagctagtt taggaggtgt
     1981 atttctttca attggtcatt taaacttttt tgcttgggga atgaccagtg gtcggggctt
     2041 tatagccctg gcagctatga ttttaggaaa atggactcct tttggttgct tggcggctag
     2101 ttttctcttt ggcataaccg atgctttaca gatgagactt caagctctcg gtattcttcc
     2161 ctcacagatt atcct
//
LOCUS       DAOSOL010000244         2170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_50594_length_2170_cov_2.914894, whole genome shotgun sequence.
ACCESSION   DAOSOL010000244 DAOSOL010000000
VERSION     DAOSOL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50594_length_2170_cov_2.914894"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1165
                     /gene="argC"
                     /locus_tag="PK171_05780"
     CDS             134..1165
                     /gene="argC"
                     /locus_tag="PK171_05780"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOT05732.1"
                     /translation="MIRVGIAGATGYTGLELLRICSNHPEVEIIWATSDSYSGIELKN
                     YCPAWQGRTCMVLEKPDMDKMAREVDVVFLGLPHGVSMDYIPAILPHAKVIDLGADFR
                     IQNLEVYEKCYGLHHKLPTVVQEAVYGIPELYRQKIKAAQVIANPGCYPTSIIIPLYP
                     LLKNQIIHPEIIADSKSGVTGAGKRPTEVTHFCEVDENFKAYKIGEHRHQPEIQEQLS
                     KAYGDSVEIIFTPHLLPIKRGILSTIYVQGDPSISEKEMWNVIDNFYQNEPWVRVYPL
                     GQFPEIKWVLGSNFCDIGLKQVGKNHFVIVSAIDNLTKGASGQAVQNMNVLFGIDERV
                     GLPSSVLYP"
     gene            1178..>2170
                     /gene="argJ"
                     /locus_tag="PK171_05785"
     CDS             1178..>2170
                     /gene="argJ"
                     /locus_tag="PK171_05785"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOT05733.1"
                     /translation="MEKEIIQKSGGLDIVEGFFTRGVHCGIKKSNNDLAIIFSEIPAA
                     AAAVFTTNRVCAAPVELTRMNLEKNHHIQLVVVNSGVANACTGTKGMEDAILMSQMAA
                     REFNIEDPSLVAVASTGVIGDFLPLNKIEIGIKQTREFYPDNPSGYDTARAIMTTDTF
                     LKEKAVTFFLDGQEVHLAGIAKGSGMIKPNMATMLGFILTDAEIDPRSLQKALKNAVD
                     LSFNRVTVDSDTSTNDMVLLLANGKRGKGVINEKHPDFLIFQEALNRVCRDLAKDIAR
                     DGEGATKFINVFVQKAKNELEARTVAFTIAESPLVKTAFFGEDPNWGRIMAAVGR"
BASE COUNT          649 a          377 c          509 g          635 t
ORIGIN      
        1 acccctccaa agaggggaat tgttgaattc attctttttc ttttttcatc acttttcact
       61 tatttcggga ttttcagaat agaataaacc actgaggaga atgacgatta atctaaaaaa
      121 ggagataagc aagatgattc gggtgggaat agcaggtgca actggttata ctggtcttga
      181 gttgctgcgt atttgctcaa atcatcccga agttgaaatc atttgggcaa ccagtgatag
      241 ctactcgggg atagagctta agaattattg tccggcatgg cagggtcgaa cctgtatggt
      301 tcttgaaaaa ccggacatgg ataaaatggc tcgagaagtt gatgtggtat tcttaggatt
      361 accccatggt gtttcgatgg attacattcc agctattctt ccccatgcca aggtcatcga
      421 tctgggagcg gattttcgta ttcaaaatct tgaggtatat gaaaagtgtt atggtttaca
      481 ccataagctt cctacggttg ttcaggaagc tgtttatggg ataccggaac tataccgcca
      541 aaaaataaaa gctgctcagg tcattgccaa tccaggatgt tatccaactt caatcattat
      601 tcccctttat cctttattaa aaaatcaaat tattcaccct gagattatag ctgatagtaa
      661 gtcgggagtg actggtgcag ggaaaaggcc aaccgaagtg acccattttt gtgaagttga
      721 tgaaaatttt aaagcctata agattggcga acaccgccat caaccggaaa tacaagaaca
      781 gctctccaaa gcttacggtg attcggttga gattatattt actcctcatc tccttcctat
      841 taagaggggg atattgagta ccatctatgt tcagggagat ccatcaattt cagaaaaaga
      901 gatgtggaat gttatagata atttttatca aaatgagcca tgggtgaggg tatatcccct
      961 tggtcaattt ccagaaataa agtgggtatt aggttcaaat ttctgtgata tcggtttaaa
     1021 gcaagttggc aaaaatcatt ttgtcattgt gtcggccatt gataacctaa ccaagggagc
     1081 gtctggccaa gcggttcaaa atatgaatgt cttatttggg atcgatgagc gagttggatt
     1141 accgagttca gtgctctatc catagggggt atcagtagtg gaaaaggaaa taattcaaaa
     1201 atcaggtggt ttggatattg tagaggggtt ttttacccga ggagtgcatt gtggaataaa
     1261 gaaatcgaat aatgatttag caataatttt ttctgaaatt ccagcagccg ctgctgcggt
     1321 ttttacaacc aatcgggtgt gtgctgctcc agttgagcta acccgaatga acttggagaa
     1381 aaaccatcat attcaattgg tagtggtgaa tagtggggtt gccaatgcct gtaccggaac
     1441 aaaaggtatg gaggatgcga tattgatgtc tcaaatggca gcccgagaat ttaatattga
     1501 agatccttca ttggttgcag tcgcttccac cggggtgatt ggtgactttc tccctttaaa
     1561 taaaatcgaa attggtatca aacaaacccg agaattttat cctgataatc catcgggata
     1621 tgatactgct cgggcaatta tgacgactga tacttttcta aaagaaaaag ccgttacttt
     1681 ttttcttgat ggtcaagaag tccatttagc aggaattgca aagggatcgg gaatgattaa
     1741 gccaaatatg gcaaccatgc tcggttttat cttaactgat gcggaaattg atccaaggag
     1801 tctccaaaaa gccttaaaga atgcagtaga tttgagcttt aaccgggtta cagttgatag
     1861 tgacaccagt accaatgaca tggttttgtt gttggctaac gggaaaagag gaaaaggcgt
     1921 gattaacgaa aaacatcctg atttcttaat ttttcaagaa gctcttaatc gagtttgtcg
     1981 ggatttggct aaagatattg ctcgagatgg agagggggcg actaaattca tcaatgtctt
     2041 tgtccaaaag gcaaaaaatg aattagaagc tcgaacggtg gcgttcacga tagctgagtc
     2101 acctctggtt aaaaccgctt tctttggtga agaccccaat tggggacgga ttatggcagc
     2161 agttggtcgg
//
LOCUS       DAOSOL010000245         2168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_50657_length_2168_cov_3.424042, whole genome shotgun sequence.
ACCESSION   DAOSOL010000245 DAOSOL010000000
VERSION     DAOSOL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50657_length_2168_cov_3.424042"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PK171_05790"
     CDS             <1..745
                     /locus_tag="PK171_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT05734.1"
                     /translation="FIKASAHTVKRLMLELGGHSPFIVFADADLNRAVKDGVKRAFRN
                     TGQICNSVNRIFVEKSVYDQFVKNFVEGTKKIRIGDPFEDPVPDCGPMVNQSGVKRME
                     EFIQDAKTHGGRIECGGKRPEDPKFSKGCYFEPTVITNVTPSMKIMMDEPFGPIVAID
                     SFSSVEEAVIKANNTRYGLVAYVYTSDIKKAGWVAENLEWGNVAINNISPDSLYAPYP
                     GWKESGIGLELGHYGLDQYLEWKNIKIEI"
     gene            940..1308
                     /locus_tag="PK171_05795"
     CDS             940..1308
                     /locus_tag="PK171_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05735.1"
                     /translation="MSLFHGGIDYWHSCGRIDPVLKDIMEIPSLKMVHISPWTDIEKA
                     VSVANHDIILEIVLNPVDDVEKATSQEMKEKLRRIKDCCQGLHYTVRADAFQIVSTLE
                     NDLKQIKQWIEIAREELSYK"
     gene            1551..>2168
                     /locus_tag="PK171_05800"
     CDS             1551..>2168
                     /locus_tag="PK171_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05736.1"
                     /translation="MTSRERVLAAINHKEPDRVAVDFGGHRSSGIMAIAYARLKKYLG
                     ITSGDIYVYDIPQQLAIVEPEVLDHFHIDVIELGRGFGLEKEYWKEWELPDGTPCKIP
                     VHINLVKENGDWYACSDDGTRIATQPKGSLYFDQIYFPLLGVKDLKSIDFDAAFEKSM
                     WNKLSNPPAPLGYDSEGLQKLKEGAKKLRESTDRAIVGLFGGNLHE"
BASE COUNT          689 a          341 c          492 g          646 t
ORIGIN      
        1 atttatcaaa gcttctgctc ataccgttaa aagactgatg ttggaattgg gtgggcattc
       61 tccctttatc gtttttgctg atgctgattt gaaccgagcc gttaaagatg gtgttaaaag
      121 agccttccga aatactggtc aaatctgtaa ttcagtcaat cgaatttttg ttgaaaaaag
      181 tgtatatgac cagtttgtga agaattttgt tgaagggacc aaaaaaatac gtatcggtga
      241 tccctttgag gatccagttc ctgactgtgg accaatggtc aatcaatccg gtgtgaaaag
      301 gatggaagaa ttcattcaag atgcgaaaac tcatggcggt cgaattgaat gtggtggaaa
      361 gagaccagag gatccaaaat tcagcaaagg gtgttacttt gagccaacag ttattactaa
      421 tgttacaccg tcaatgaaaa tcatgatgga tgaacccttt ggaccgatcg ttgctatcga
      481 ttctttttcc agtgttgagg aagcggttat taaagctaat aacactcggt atggtttagt
      541 tgcctatgta tataccagcg atatcaagaa ggctggatgg gtcgcagaaa atctggaatg
      601 gggaaatgtg gccataaata atattagtcc ggacagtttg tatgcacctt atccgggatg
      661 gaaagaaagt gggattggtt tagaactcgg tcattatgga cttgaccaat atttagaatg
      721 gaaaaatatc aaaatagaaa tttagttgaa agtcagttat aagcttttaa ttgctggtat
      781 ttttatacca atgattggaa agcaaaatac aaaatcttaa aaaatactcg gaggtttttt
      841 agccgggttt atctcaggat aaaagggggt gatgggttat tcattaggac cctgtgtgat
      901 tatgagggtg tgaatgatat tttattattt aataaaaaaa tgagtctttt ccatggaggg
      961 attgattatt ggcatagctg cggtcggatt gatccagttt taaaagatat tatggagatc
     1021 ccttccttaa agatggttca cattagcccc tggaccgata tagaaaaagc tgtgtcagtt
     1081 gccaatcatg atatcatttt ggaaattgtg ctcaatccgg ttgatgatgt ggaaaaagca
     1141 acctctcaag agatgaagga gaaactgaga aggataaaag attgctgtca gggtcttcat
     1201 tacactgttc gtgctgacgc ttttcaaatt gtcagcactc ttgaaaacga cttgaaacag
     1261 atcaagcaat ggattgaaat cgctcgcgaa gaattatcct ataaataggt aaaattgtcc
     1321 cagtatggtg tatgaaattg aataaaaaat cgaaatgttt tgtttaccct ctcccgccaa
     1381 ggggagagaa tattgtatat ccccaatttt tcaggaataa ttacctctgg ctcattataa
     1441 gcttcttgta gcattcaaga gaaagacagg aataaatata aaataatgtt aagatatttg
     1501 ataacaatga aataaattaa aaaattatct acaattggga ggtgacagga ttgacctcac
     1561 gagaaagagt gctggctgca atcaatcata aagaaccaga tcgggtcgca gttgattttg
     1621 gtggtcatcg atccagcggt attatggcaa ttgcctatgc ccgtttaaaa aagtatttag
     1681 gaattaccag cggggatatc tatgtttacg acattcctca gcaattagct attgtcgagc
     1741 cagaagtgtt agatcacttt catatagatg tcattgaatt aggacgtggt tttggtttgg
     1801 aaaaagagta ctggaaagag tgggaacttc ccgatggaac cccttgcaaa attccagtcc
     1861 atatcaattt ggttaaggaa aatggtgatt ggtatgcctg ctctgatgat ggaactcgaa
     1921 ttgctactca accgaaaggt tcgctttatt ttgatcaaat atatttcccc ttgcttgggg
     1981 taaaggacct aaaatcaatc gattttgacg cagcatttga aaaatcaatg tggaataagt
     2041 tatcgaatcc acctgctcct ttgggatatg attcagaagg tttgcagaaa ttgaaagaag
     2101 gagccaagaa acttcgcgaa tcgaccgatc gggcaatcgt tggtcttttt ggtggaaacc
     2161 ttcatgaa
//
LOCUS       DAOSOL010000246         2162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_50856_length_2162_cov_4.788325, whole genome shotgun sequence.
ACCESSION   DAOSOL010000246 DAOSOL010000000
VERSION     DAOSOL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50856_length_2162_cov_4.788325"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..405
                     /locus_tag="PK171_05805"
     CDS             22..405
                     /locus_tag="PK171_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOT05737.1"
                     /translation="MKILVIACDKSGYPAMIKYTTENSTNHEFIKIIKAPCLGSVKES
                     DILEALEGKCDRILLVGCPIDSCFHQNGSRFAQRRVNRINHLLEEAGITKRVAISFVT
                     AEKISEIKRTLDLISSTPVQEEHKP"
     gene            402..1052
                     /locus_tag="PK171_05810"
     CDS             402..1052
                     /locus_tag="PK171_05810"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT05738.1"
                     /translation="MIIGERKPIPEILAMLGDSKKVLVLGCGTCVTVCLTGGDKEAKE
                     LASLLRIKGYEADSLTVERQCELEFIHPLRESIQSVDAVISLACGIGVQALFELDSEK
                     SIYPGLNTTFMGMPVKQGYWEERCWGCGNCIIHLFGGTCPITRCAKSLLNGPCGGSKD
                     GKCEVKPDRDCAWQLIYDRLKNLNRLDWLLTIQPPKDWSTSRYGGHRKMTREDMLL"
     gene            1049..1975
                     /locus_tag="PK171_05815"
     CDS             1049..1975
                     /locus_tag="PK171_05815"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOT05739.1"
                     /translation="MSWLKQVLQSGHFAVTAEVGPPKGSDPQVIRKKCDLLKGFVDAV
                     NITDNQTAIVRMSSFASCLIAQKQEIEPVMQMVTRDRNRIALQSDFLGACALGISNLL
                     CLTGDHQSMGNHPQSKNVYDIDSIQLLQIFKNMRDQKVFQNGEEVKGEINVFLGAGES
                     PYADPLEFRALRLAKKIAAGAEFIQTQAIFDVDIFTQWMEEVCCLGLHKKSFILAGVI
                     PVKSAKALRYMKNEVPGVVIPDNLIERMEAATDQKAEGVEVCIETIQKVAKIEGVSGV
                     HIMAIAWESIVPEIVQRSGLLPRPAKGEITSS"
     gene            1972..2133
                     /locus_tag="PK171_05820"
     CDS             1972..2133
                     /locus_tag="PK171_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05740.1"
                     /translation="MKAEKKFDPQFKYQVASRPGGEGLMACFACGVCTAGCPVSEVES
                     DFNPRRIIH"
BASE COUNT          642 a          403 c          518 g          599 t
ORIGIN      
        1 ctgtttttgg agtgatggag attgaaaata ttggtaatcg cttgtgataa aagtggctat
       61 ccagcgatga taaaatatac cactgaaaat tcaaccaatc atgaatttat caaaattatt
      121 aaagcgccct gccttggaag tgtaaaagaa agtgatattc ttgaagcctt agaagggaag
      181 tgtgatcgga ttctcttggt tggctgcccc attgatagct gctttcatca aaatggaagt
      241 cgatttgctc aaaggcgagt aaatagaatc aatcatctgt tagaagaagc tggtattacc
      301 aaaagagtag ctatcagttt tgtaactgcc gaaaaaatct ctgaaataaa aagaactttg
      361 gatttgatct cgagtactcc agtccaagag gagcataaac catgatcatt ggtgaaagaa
      421 aaccaatacc agaaatcctt gctatgcttg gcgattcaaa aaaagtcttg gttttaggat
      481 gcggaacttg tgtaaccgtc tgtctcaccg gtggtgataa agaagccaaa gaattagcct
      541 ctttattgag aataaaagga tatgaagccg atagtttaac tgtcgaacga caatgcgaat
      601 tggagtttat ccatccttta cgggaaagta ttcagtcggt tgatgccgtc atttctctgg
      661 cctgcggcat tggtgttcaa gctctttttg agctcgattc tgaaaaaagt atctatcctg
      721 gattaaatac caccttcatg ggaatgccgg taaaacaagg gtattgggaa gaacgttgtt
      781 ggggatgcgg gaattgcatc attcatcttt tcggtggaac ctgcccgata acccgctgtg
      841 ctaaaagtct cttaaacggt ccttgtggtg gttcaaaaga cgggaaatgt gaagtgaaac
      901 ctgatcgtga ttgcgcttgg cagttgattt atgatcgcct aaagaatctc aatcgtttgg
      961 attggttgct gacaattcaa cctccaaaag actggtcaac atcccgctat ggtgggcatc
     1021 gtaaaatgac tcgggaggat atgttgttat gagctggctc aaacaggtgt tgcaaagtgg
     1081 tcatttcgcc gtcactgccg aagtgggtcc gcccaaggga tccgatcctc aggtaatacg
     1141 aaaaaaatgc gatctgctca aaggttttgt tgatgcagtc aatatcaccg ataatcagac
     1201 ggctatcgtc cgcatgtcaa gttttgctag ttgtttgatt gcccagaagc aggaaatcga
     1261 accggttatg caaatggtca cccgagaccg aaatcgcatt gcactgcaaa gcgattttct
     1321 cggggcttgc gctttaggta tttcaaatct tctctgcctt accggtgacc atcagagtat
     1381 gggaaaccac ccccaatcaa aaaatgtcta cgatattgat tcaatccagc ttctacaaat
     1441 ctttaaaaat atgcgtgatc agaaggtatt tcaaaatgga gaggaagtga agggggaaat
     1501 taatgttttt ttaggtgcgg gtgaaagccc atacgctgat cctctcgagt ttcgtgctct
     1561 tcgcttagcg aagaagattg ccgccggagc cgaatttatc caaactcagg caattttcga
     1621 tgtggatatt ttcacccaat ggatggaaga agtgtgctgc ttaggacttc ataaaaaatc
     1681 atttatttta gctggagtta ttccggtcaa atcagcgaaa gctttaaggt atatgaaaaa
     1741 cgaagtgcca ggtgttgtca ttcctgacaa tttaatcgag agaatggaag cagccactga
     1801 tcaaaaggct gaaggcgttg aagtttgtat cgaaaccatt caaaaagttg cgaaaataga
     1861 aggagtatct ggagtccaca ttatggccat tgcttgggaa agtatagttc cggaaatcgt
     1921 acaacggtct ggcctattac cacgaccggc aaaaggagag atcacttcat catgaaggct
     1981 gaaaaaaaat tcgatcctca attcaaatat caagttgcgt cgcgacccgg tggagaggga
     2041 ttaatggcat gttttgcctg tggagtgtgt accgccggat gtccggtaag tgaagtggag
     2101 agcgatttta atccccgccg aattattcat taaatcttag ttggagatcg tgagggaatt
     2161 tt
//
LOCUS       DAOSOL010000247         2143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_51490_length_2143_cov_4.150383, whole genome shotgun sequence.
ACCESSION   DAOSOL010000247 DAOSOL010000000
VERSION     DAOSOL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51490_length_2143_cov_4.150383"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1183)
                     /locus_tag="PK171_05825"
     CDS             complement(152..1183)
                     /locus_tag="PK171_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT05741.1"
                     /translation="MKWGVIGAGGIARRRSIPEVVKFSEKSQITALMDVSKELIEETG
                     KQFGIDQCYTSVEEIFKQDIDAVYIASPVFEHEKQVAAALKAGKHVLCEKPMALTLDQ
                     TEAMVELAEKKNLKIGVAFMMRYNVYHQKIKKLVEENQLGQVVYGRAQLTCWYPPIPG
                     AWRQKKVTGGGGALIDMGCHCIDLLEWIIGPCQEVTAFIDTITHSYEVDDTSTILMKF
                     KNGAQGVVDNFFNIPDQAAKNMLEIYGTKGAVITHNTIGQDSAGKMWLYLESGDQGYS
                     SQQARTQDAYQEVVLEPKSMYAQEFDAFSDWIQTGVKPVISYEAGIRNFKIIEAAYQS
                     SQERKAIDV"
     gene            complement(1651..1726)
                     /locus_tag="PK171_05830"
     tRNA            complement(1651..1726)
                     /locus_tag="PK171_05830"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1691..1693),aa:Gly,seq:tcc)
     gene            complement(1805..>2143)
                     /locus_tag="PK171_05835"
     CDS             complement(1805..>2143)
                     /locus_tag="PK171_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HOT05742.1"
                     /translation="GIEMQLPGRVGDSPLIGLGNFAGEWGAVSMTGAGEGIIKLGLAR
                     KIADWMEEMSAQSAVDRGMALANKYNVVCGAIALSYREEFGIGNNGGKISYSYWKDGM
                     QEPYTYKDSQ"
BASE COUNT          597 a          504 c          351 g          691 t
ORIGIN      
        1 gaggggtcga aagctcgcgc tttcctctca gccccgcact atggagctcc ccactaaaac
       61 acttattcaa ttttccttac ttttataaac tatcacttga taattgaaat gtcaataatt
      121 atatccattt atggctcatt ccatctatac cttagacatc tatcgctttt ctttcttgtg
      181 aagattgata agctgcttca atgattttaa agttacgaat cccagcttca tagctaataa
      241 ccggctttac cccggtttga atccaatcac taaaggcatc aaattcctgg gcatacatgg
      301 attttggttc taatactact tcttggtagg cgtcctgggt gcgagcctgc tgggaagaat
      361 atccctgatc ccctgattca agataaagcc acatttttcc tgccgaatcc tggccgatgg
      421 tattatgagt aattacagca cctttagtcc cgtatatttc caacatattt ttagctgcct
      481 gatcagggat gttgaaaaaa ttatcaacga ctccttgagc tccgttttta aacttcataa
      541 gaatggttga tgtgtcatct acttcgtagg aatgggtaat tgtatcaata aaagccgtca
      601 cctcttggca gggaccaata atccattcta aaaggtcgat acaatgacaa cccatatcaa
      661 taagagcgcc accgcctccg gttacttttt tttgcctcca agcaccagga ataggtggat
      721 accagcaggt caattgagct cgaccataaa cgacctgacc taattgattt tcttctacca
      781 gcttttttat tttttgatga taaacattgt agcgcatcat gaaagccact ccaattttca
      841 gattcttctt ttcagccaat tcaaccattg cttcggtctg atcgagggtt aaagccatcg
      901 gtttttcgca taaaacatgt ttaccggctt tcaaggctgc tgccacttgt ttttcatgtt
      961 caaaaaccgg tgaagcaatg tatactgcat ctatatcttg tttaaaaatt tcttcaacac
     1021 tggtgtagca ttggtcaatt ccaaactgct ttcccgtttc ctctattaat tctttagata
     1081 catccattaa tgcagtaatt tggctctttt cggaaaattt taccacctca ggtatacttc
     1141 ttctccgcgc gattcctcca gctccgatca ctccccattt caccttcaaa ctgaacacct
     1201 ccgtttttaa tttttttatc aattctattt tatagcttta tttcagtttt gattagaacc
     1261 atatattact actttactaa tctgagaata taataataag aattaaatgg tgtcatccta
     1321 ggcggtgctt tcccacgtga ggatctcaat tttttctttt taattcttaa ctattttaaa
     1381 aaatgagatt ctcacgtcgt ccggtaaaag gcaccagaca cctcagaatg tcggaacgga
     1441 tgggtgagat tgccacatca tccgaccaaa gaacggttgg attcctctct atgacggttt
     1501 taattaggtc ttttcatcct catctattgc cgttacatgg cattaacacc tatcccagca
     1561 aaaaaattgt taaactaaaa tcaatattca aactctgggt gttttattta ttcgtagttt
     1621 ttcgaggaaa gcgaagttat tctattaaaa tggagcggga aacgggactc gaacccgcga
     1681 caaccagctt ggaaggctgg agctctacca actgagctat tcccgctcaa ttttcctgat
     1741 ggggattata acacaccagg actattcgtc aagattttat aaccaagaag aaataaataa
     1801 atttttattg agaatcctta taggtgtaag gttcttgcat cccatccttc caataggaat
     1861 aactaatttt ccctccatta tttccaatac caaactcttc tcgatagctg agggcgatag
     1921 ctccacaaac aacattatat ttatttgcta aagccattcc tcggtcaacc gcactttggg
     1981 cactcatttc ttccatccaa tcggcaattt tccgagctaa acctaattta ataattccct
     2041 cgccggctcc agtcatcgac accgcacccc attctccggc aaagtttcct aaaccaatta
     2101 aaggagagtc acctactcga cccgggagct gcatttcaat tcc
//
LOCUS       DAOSOL010000248         2133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_51813_length_2133_cov_3.027911, whole genome shotgun sequence.
ACCESSION   DAOSOL010000248 DAOSOL010000000
VERSION     DAOSOL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51813_length_2133_cov_3.027911"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1297)
                     /locus_tag="PK171_05840"
     CDS             complement(<1..1297)
                     /locus_tag="PK171_05840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05743.1"
                     /translation="MDYILEATGITKIFPGVVALNEVDFACKKGTVHCIVGENGAGKS
                     TFVKVLTGIYHPEKGNVKIEDKSIFENPEIINKVAYVPQELQLFEYMTVGENLLLPFK
                     RKGLNRFYSSKSKIFKMAAPLLEKFHVAEKAETSVNDISPSSKQLLQIARALIIEDCE
                     IIILDEPTTSLTIEDTKRLFQVVNQLKKEGKAIIYISHKLEEVFAIGDEITVLRDGKK
                     VGYSKASDVDRNWIIKMMSGKEIDERIIFRPTKNQGELILEVKNLTGKGFLNINFELH
                     RGEILGFYGLVGAGRSEIMQTILGYRYVAEGEVQVDGKPLKLGNPHFAIQGGVFYLPE
                     ERKQQGIFPYLSVRHNVGVALGEKVLNGVVVSAKKEKSMTEELVKVYSVKTSSIETPI
                     RFLSGGNQQKVIVGRAMFCSPTPKVIIFDEPTKGIDVMTK"
     gene            complement(1398..>2133)
                     /locus_tag="PK171_05845"
     CDS             complement(1398..>2133)
                     /locus_tag="PK171_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05744.1"
                     /translation="NRILEQAAATEPAGITLDLLDAEGNRPVLEAILARGIPIVVFDS
                     YAPEGMGLTTVNNDFVAQQIGACQRLVDLMKAKEKKDVYKVALIEGVPTAPNHKKRFE
                     TSKEFFSKLPEIEIVAEGVDNDSIEQAQKQAAAIIAAHPDLDGMIAHNAAGPIGVGLA
                     IKEAGKVGEIIHVGLDDLDQLIVLIKEGVVESSYSTKPKMQGAYAVLCMWLQNQGIAT
                     PMLVDTGFVVITKDMIPDDVKEYKGY"
BASE COUNT          600 a          466 c          327 g          740 t
ORIGIN      
        1 ttttcgtcat gacatcaatt cccttggttg gttcatcaaa aatgattact tttggagttg
       61 gcgaacaaaa cattgcacgt ccaacaataa ctttttgttg atttcctcca cttagaaatc
      121 ttatcggtgt ttctattgaa gatgttttta ccgaatatac ttttaccaac tcttcggtca
      181 ttgatttttc tttctttgct gataccacaa ccccattaag tactttttcc ccgagagcaa
      241 ccccaacatt atgacgaaca cttaaatatg gaaatatccc ttgttgcttt cgttcttcag
      301 gaaggtaaaa tactccacct tggatggcaa aatgagggtt cccgagcttt aacggttttc
      361 catcaacttg tacctctcct tcagccacat atctataccc caaaatagtc tgcataattt
      421 cagaacgccc tgctccaact aaaccgtaaa atcccagtat ttctcctcgg tgtagttcaa
      481 aattaatatt taaaaaccct ttaccggtta gattttttac ttctaatata agttctcctt
      541 gattcttagt aggacggaag ataattcttt catcaatttc tttccccgac atcattttta
      601 ttatccagtt tctatcaaca tctgaagcct ttgaataacc aacttttttc ccatctctga
      661 gaacggttat ttcatcacct atagcaaata cttcttcaag cttatgagat atatatatta
      721 ttgctttccc ctctttcttg agttggttta ctacttgaaa tagacgcttg gtatcttcaa
      781 tggtcaaact ggttgttggc tcatcaagga ttataatctc acagtcttcg atgattagcg
      841 cacgagctat ttgcaataat tgtttactcg aaggagaaat atcatttacg ctagtctcag
      901 ctttttcagc aacgtggaat ttttctaaga gaggggctgc cattttaaat attttacttt
      961 tactcgaata aaatcgattt aaccctttcc ttttaaaagg taataataaa ttttctccta
     1021 ccgtcatata ttcaaataat tgtaattctt gaggaacata agcaacctta ttaataattt
     1081 cggggttctc aaagatactt ttatcttcaa tttttacatt tcctttttca ggatgataaa
     1141 taccagttaa aacttttaca aaagtacttt taccagctcc attctctcct acaatacaat
     1201 ggactgttcc ttttttacaa gcgaaatcga cttcattgag agccactacc cctggaaaaa
     1261 ttttggtaat accggttgct tctaagatat aatccatttt ttcttcctgt cctcatccat
     1321 ttttattaaa aaccacccat aaaaagttga acctaaaaag agcaactttt tatgggtgat
     1381 acaataatta gttcctttta gtatccttta tattccttta catcatcagg aatcatgtct
     1441 ttggttatta caacaaaacc agtatcaact aacattggcg tagcaattcc ttggttctgt
     1501 aaccacatac aaagaactgc atacgcacct tgcattttgg gtttggtact atatgaagat
     1561 tccacaacgc cttcttttat caaaacaatc agttggtcta aatcatccag gccgacatgg
     1621 atgatttctc caacttttcc agcctcttta atggcaagac cgactcctat tggtccagct
     1681 gcattatggg ctatcatacc atcaaggtca gggtgggcag caatgattgc agctgcttgc
     1741 ttctgagctt gttcaatgct atcgttatca acaccttcgg ctacaatttc tatctctggt
     1801 agcttggaaa aaaactcttt tgaagtttca aaacgtttct tatgattggg tgcagtagga
     1861 actccttcaa tgagagctac tttataaaca tccttctttt ctttagcttt catcaaatcc
     1921 acgagtctct ggcaggctcc aatctgttgg gcaacaaaat cattgttaac cgtggttagg
     1981 cccatgcctt ctggagcgta ggagtcaaat acaacaatgg gaattccacg ggcgagaatt
     2041 gcctctaaga ccggcctatt tccttcagca tcaagaaggt ccaaagtaat acccgctggt
     2101 tcagttgctg ctgcttgttc aagtattctg ttt
//
LOCUS       DAOSOL010000249         2131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_51865_length_2131_cov_3.509634, whole genome shotgun sequence.
ACCESSION   DAOSOL010000249 DAOSOL010000000
VERSION     DAOSOL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51865_length_2131_cov_3.509634"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PK171_05850"
     CDS             <1..274
                     /locus_tag="PK171_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05745.1"
                     /translation="TLVISPEVSNVVGAGIQDLPVISRRIASTTVRVKDGETIVIGGL
                     RQLIEVTTKTKVPILGDIPLIGGLFRNKKQTVDDKDIVILITPKII"
     gene            346..2124
                     /locus_tag="PK171_05855"
     CDS             346..2124
                     /locus_tag="PK171_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05746.1"
                     /translation="MLSLSPNSVYAISATDQPKIDLFCPDISIKEALLTAAFQADVEI
                     VFFQNIPGKVDIRKDGVTFEKALDLILKNTDVSWYLIDGVYYIGTPPVGSSAYIKISS
                     PEKYPLNYISSKELFKLLPQYAGNLIIDAPYSVILSGPEGLREEIKKEIEKLDHPKEH
                     IQVKSIITEVKNETLQDIGLDVTAGGDQNLQIGSAASIGGSFERLEKIDAVLRALQES
                     GEAVIHSESEIIVLEGEEGRIWSGKDVYYSIETEGTAGTTSNLEIIELGAGLRVTPQY
                     IGEGKIKINLETQFKNLENENETLPVIISRDTVTSVTLIEKEPLFIAGVNTTKTSEIR
                     NKLLEKNKDAESVNLEEESQLVVFLEAERKTPQESPLVVKLKSEDIKVSPSTIRKTTT
                     EYQFGLGAWYGTLIIPEYQTTPQFWYGLEVKVLSGENFGLYGQLLKASDKNLWLGKVS
                     LEYAVHTDEDTPETINLGIGLKGFWGDVQESAFVAYVKDIRRVEENLLTFFSIGLQFS
                     ENESLYFASLGASYFENEWALEAEAKYQWNNRASNWILGIEGKYRLDNKTYLVAGYRD
                     IISGSPIEPFDDFDFRGLYAGVTFMF"
BASE COUNT          694 a          376 c          445 g          616 t
ORIGIN      
        1 taccctcgtt atttcacctg aagtgagtaa tgtggtagga gccggaattc aggatttacc
       61 agtgatcagc cgccgcattg cctctactac tgtccgtgtt aaagatggtg aaaccattgt
      121 cattggagga ttaaggcaac tcatcgaggt caccacgaaa accaaagtcc caattttagg
      181 tgatatcccg ctcattggag gcttgtttcg gaataaaaag caaaccgtcg atgataaaga
      241 tatcgtgatt ctgattacac caaaaatcat ttagaaaaga gaggaattat gagtcgaaga
      301 tggtgcaaaa aatatttcat tgttcatcta cttcttatat atttcatgct ctctttatcg
      361 ccaaattctg tatatgcaat ttctgctaca gaccaaccga aaattgatct tttttgccca
      421 gatatttcaa ttaaagaagc actgcttaca gctgcttttc aagctgatgt tgaaatcgtt
      481 ttttttcaaa acataccggg aaaagtcgat atacgcaaag atggagtcac ctttgaaaaa
      541 gcactcgatc tcatactgaa aaacaccgat gtcagctggt atttgatcga tggagtatat
      601 tatatcggta ctccgccagt cggaagttca gcttatataa aaattagttc cccagaaaag
      661 tatcctttaa attacatctc cagcaaggaa ctttttaaac tactccctca gtatgcgggc
      721 aatttgatca ttgatgctcc atattcagta attctttccg gaccagaagg tttaagagaa
      781 gaaattaaaa aagaaattga gaaattagat caccccaaag aacatatcca ggttaaatcc
      841 ataattaccg aagtcaaaaa tgagactttg caagatattg gccttgatgt gactgccggt
      901 ggcgatcaaa acttacaaat cggttctgcg gcttcaatag gaggaagttt tgaaaggctg
      961 gaaaaaatcg atgcggtttt gcgtgccttg caggaaagcg gtgaagcagt tatccatagt
     1021 gagtcagaaa ttatagtttt agagggagaa gaagggcgca tttggtctgg aaaagatgtc
     1081 tattattcaa tagaaaccga aggcactgct ggaacaacca gcaatcttga aattattgaa
     1141 ttaggagctg gtctcagagt aactccccaa tatatcggtg agggaaaaat taagattaat
     1201 ttagaaaccc agtttaaaaa tcttgagaac gaaaatgaaa cacttccggt tattatcagc
     1261 cgggatacag ttacttcggt tacattaatc gagaaggaac ctctttttat cgctggtgtc
     1321 aatacaacga aaaccagcga aattagaaat aaattgctgg aaaaaaataa agatgctgaa
     1381 tcagtaaatt tagaagagga aagtcaactg gtggtttttt tggaagccga aagaaaaacc
     1441 ccccaagaat ctcctctggt ggttaaatta aagagtgaag atataaaagt tagtccctca
     1501 actattcgca aaacgacaac cgagtatcaa ttcggtttag gtgcctggta tggaactctt
     1561 ataatccctg aatatcagac cacaccacaa ttctggtatg gtttagaagt taaagttcta
     1621 tctggtgaaa attttggact ttatgggcag cttttaaaag cttctgataa aaacctgtgg
     1681 ttagggaaag taagcttgga atatgctgtt cataccgatg aagacactcc cgaaactatt
     1741 aacctgggaa ttggtctaaa aggtttttgg ggagatgttc aagagtcggc gtttgttgct
     1801 tatgtaaaag atattcgtcg agttgaagag aatctcctaa ctttcttttc aataggctta
     1861 caattttccg aaaatgaaag tctctatttc gcttctcttg gagcatccta ctttgaaaac
     1921 gaatgggctc tggaagctga agcgaaatat caatggaata accgagcttc aaattggata
     1981 cttggtatcg agggaaaata tcggttagac aacaagacct atctcgttgc tggttatcgg
     2041 gatattatca gtggttcgcc aatagaacct tttgatgatt tcgatttccg agggttgtat
     2101 gccggtgtga catttatgtt ttaaataaat a
//
LOCUS       DAOSOL010000250         2126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_52036_length_2126_cov_4.225012, whole genome shotgun sequence.
ACCESSION   DAOSOL010000250 DAOSOL010000000
VERSION     DAOSOL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52036_length_2126_cov_4.225012"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PK171_05860"
     CDS             complement(<1..859)
                     /locus_tag="PK171_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05747.1"
                     /translation="MKSKTIVSVSLGSSLRNHQAEIELMDFRINIKRIGVDGDKKKFM
                     QVLRELDGKVDAFGLGGADLYLRAGRYKYQVVDIAKMVSVLEKTPIVDGGELKETWER
                     KVPQILVEKEKLDLQGKTALFMSGMDRYGLAEGLSQQGCRLLIGDMPFALGIPIPLTR
                     LSTLRFLSIIMMPVLRRLPLKVLYPTGKSQETRVTRSLHLFKRADIVAGDFLYINRFM
                     PDDMKGKIIITNTVTNNDIETLRKFGVKILVTTTPEMNGRSFGANVLQAIFVSILEKH
                     PDKILPEEYL"
     gene            complement(849..1208)
                     /locus_tag="PK171_05865"
     CDS             complement(849..1208)
                     /locus_tag="PK171_05865"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOT05748.1"
                     /translation="MNLIKSHENVKDIAILGDQTRLDGHYQGEGTVMIQGEFRGSIDS
                     MNVIITRHGQSKAFIQAKNLEIWGTLAGFTRTEKVICRSSCRITGIIMSNGIAIEPGT
                     TFEGGLTFPQTGDSGEK"
     gene            complement(1186..1965)
                     /locus_tag="PK171_05870"
     CDS             complement(1186..1965)
                     /locus_tag="PK171_05870"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOT05749.1"
                     /translation="MNKNMLLTIDVGNTHTTFGVFHYEKLLADWRVSTSSRRTSDEWG
                     YILKELITDQGIEKKNINQVAISCVVPPVLNTLRELFEKKWINILMVIGPGVRTGLSI
                     RAEPKETGADRIVNAVAAAHLYGGDLVIIDFGTATTFCAINTSREYLGGSIAPGIGIS
                     QEALYEKTARLPRIDVEIPDKCIGRNTVEAMHSGIFYGYIGLCREIVMNMKKEFAPKA
                     QVVATGGWGTFLHSYCDFIDFFNPTLTLEGLRIINELNQKS"
BASE COUNT          655 a          459 c          331 g          681 t
ORIGIN      
        1 gtaaatattc ctcaggtaag atcttatcag gatgtttctc gagtattgaa acaaaaattg
       61 cttgaagtac attcgcacca aaagaacgtc cattcatctc aggagtagtt gtaacaagta
      121 tttttactcc aaattttcga agagtttcga tatcattatt tgtgaccgtg ttggtaataa
      181 taatcttccc tttcatatcg tccggcataa atcgattgat atataaaaag tctccagcta
      241 cgatatcggc tcttttaaat agatgaagag atcgagtaac tcgtgtttct tgactttttc
      301 cggttggata tagaactttc aaaggaagac gcctaagcac cggcatcatt ataatggata
      361 gaaagcgaag ggtagaaagc cgagttaggg gaatgggtat tcctaacgca aatggcatat
      421 ctcctatcaa aagccgacaa ccctgctgag aaagtccctc tgctaaacca taacgatcca
      481 tcccactcat aaaaagtgcg gttttccctt gtaaatctaa tttttccttt tcgactaaaa
      541 tctgtggaac ctttctttcc caagtttctt tgagttctcc accatcaaca attggtgttt
      601 tttccaaaac tgaaaccatt ttagcaatat caaccacttg atatttatat cggccagcac
      661 ggaggtaaag gtcagcaccc cccaaaccaa aggcatcaac tttaccatcc aattctctta
      721 aaacttgcat aaactttttt ttatcaccat caacgcctat tcttttaata ttaattctaa
      781 aatccattaa ttcgatttcg gcttggtgat tgcgaagtga agaccctaaa ctaacactta
      841 cgattgtttt acttttcacc actatcacca gtttggggga atgtcaatcc accctcaaac
      901 gttgttccag gttctatagc aattccattt gacataatta ttcccgttat tcggcatgaa
      961 ctacgacaaa taaccttttc cgttcgagta aaacctgcta aggttcccca aatttctaag
     1021 ttttttgcct ggataaaagc ttttgactgt ccatgtcggg taataatgac attcattgaa
     1081 tcaatagatc cacgaaactc gccctgaatc atgaccgttc cctcaccttg ataatgacca
     1141 tccagacgag tttgatcacc caatattgca atatctttta cattttcatg acttttgatt
     1201 aagttcatta ataattctca aaccttcaag ggtaagtgtt ggattaaaaa aatcaataaa
     1261 atcacaataa ctatgtagaa atgttcccca tcctccagta gcaacaactt gtgcttttgg
     1321 agcaaattct tttttcatat tcataacaat ttctctacat aacccgatgt acccataaaa
     1381 aatgcctgaa tgcattgcct caaccgtatt acgcccaata catttatctg ggatctcaac
     1441 atcaattcga ggtaaacggg cagttttttc atagagagct tcttgtgaaa ttccaattcc
     1501 tggagcaatt gatcccccca aatattctcg gctggtatta atagcacaaa aagtggttgc
     1561 tgttccaaaa tcgataatca ctaaatcacc accatagaga tgtgcagctg ctacagcatt
     1621 aacaattcga tcggcaccag tttcttttgg ctccgctctt attgataaac cagtacgaac
     1681 tcctggtcca attaccatta atatatttat ccatttcttt tcaaacagtt ctcgaagggt
     1741 attgaggacg ggagggacca cacaggaaat tgccacttga tttatatttt ttttctcaat
     1801 gccttgatca gttatgagtt cctttagaat atatccccat tcatcactgg ttctacgtga
     1861 ggaggttgaa actctccagt cagcaagaag tttttcataa tgaaaaactc caaaagtggt
     1921 atgggtattt ccaacgtcta tggttaaaag catattttta ttcaattcac caccttgata
     1981 tgattgatag cattataatc caataatgtc ttctctaaaa taccatttat aagtcatcta
     2041 agctatcccg taaatccctt tgtgactttg atgtatcaag cctctacaaa aaattaaaaa
     2101 acttagggat tgcaatttat tgcact
//
LOCUS       DAOSOL010000251         2122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_52185_length_2122_cov_3.199806, whole genome shotgun sequence.
ACCESSION   DAOSOL010000251 DAOSOL010000000
VERSION     DAOSOL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52185_length_2122_cov_3.199806"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..1178)
                     /locus_tag="PK171_05875"
     CDS             complement(366..1178)
                     /locus_tag="PK171_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05750.1"
                     /translation="MKALCIVILILVIVVALFLVGIHIKPRPFPPFPRSAKSILKTIP
                     LPDGLPKPVERFYQLIYGENIPVIDSAIVSGRLRLRIMGITFPGRFRFVHETGKGYRH
                     YIETTLLGFPIMKVNEFYLNGKGRLELPFGISESPEIDQGGNLGMWAESTFFPAIWLT
                     DPKVRWEPLDQDSSILVVPFGEQEERFIVRFDSQTGYLKLMEAMRYKSAGDKHKVLWL
                     AETLKWGSIDKHNLMIEGSATWLDEGKPWAVFTVDDVVYQVDVSEYLQAHGL"
     gene            complement(1216..>2122)
                     /locus_tag="PK171_05880"
     CDS             complement(1216..>2122)
                     /locus_tag="PK171_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT05751.1"
                     /translation="LMNDVFVLLGIDMETDIGSFTPFLQGVEKGTPLLLKLFEKKGIP
                     ATFLWVADIISQFKSLAKEIERCGHENGCHTLQHETLGEPYFDIPLIKPVLSEEVAYR
                     IQKATEIVGDVIGTQPLSFRTTRFWGNEITLNTLNDLGYLADSSYPMYYYQKQLTPYH
                     PSRENWTEIGNLNILEIPTFADLTIESHDEYGRDRDQWPIFRTEGSQVLMNHIENMIT
                     YFSKRNLPKVFCFYLHPWEFIEVPSRLTYGEATIEPFPFITKNCGLKALQELSILIDE
                     LLQIGGQFYTFQDFTKIWNNRLSNE"
BASE COUNT          644 a          414 c          449 g          615 t
ORIGIN      
        1 gcttgataaa tcaagcccct atttgttttc aggatagacc ctcatgccac tttcgtggca
       61 acagatgggg atgaaaatta cttattcaga tatgaatctc cccctttaga aaagggggtt
      121 ggggggattt gagttttttg gaaattaatc gaatcccgaa tcatctccct ttagccaaag
      181 ggagaggtcg attaaaaaag gaattttcat gcaactgtat gttcagaata aaagtaccta
      241 tcagctactt cgttaaataa gttttgaaaa aatttttttc ctcaccctga ctttaatttt
      301 caaaggggtg agagaaaaaa attaggttta aaagaaggag gggaaacatt ttaaatcatc
      361 agttattata acccatgagc ctgtaaatat tcgcttacgt ctacttgata gacaacatca
      421 tcaacggtaa agactgccca gggttttcct tcatcaagcc aagtggcact tccttctatc
      481 atgagattat gtttatcaat agaaccccat tttaatgttt cggcaagcca aagcactttg
      541 tgcttatcac cagcactttt atagcgcatg gcttccataa gttttaagta accagtttga
      601 gagtcaaacc gcacaataaa gcgctcttcc tgctctccga agggaacaac cagaattgat
      661 gagtcctgat cgagcggttc ccagcggact ttagggtcgg tcaaccaaat ggctggaaaa
      721 aatgtagact ctgcccacat accgagattg cctccttgat cgatttcagg actttcgctg
      781 attccgaagg ggagttccag acggcctttg ccgtttaaat agaactcgtt aactttcata
      841 attggaaaac caaggagagt ggtttcaata taatggcggt atcctttccc ggtttcatgg
      901 acgaagcgga atcgtccggg gaaagttatg cccataatac gaaggcgtag gcgaccactg
      961 acaattgctg aatctataac cggaatattt tctccatata ttaattggta aaaacgttcc
     1021 accggttttg gtaaaccgtc tggcaaggga attgttttta ggattgattt agccgatcgt
     1081 gggaaggggg gaaaaggtcg aggtttgatg tgaattccaa ctaaaaatag tgcaactaca
     1141 attaccagaa ttagaataac gatgcaaaga gccttcatgg tgacctccgt ttctaataag
     1201 atggtatctc acccatcatt cattactcag tcgattgttc caaattttag taaaatcctg
     1261 aaaagtataa aattgtcctc cgatctgaag taattcatct ataaggatgg aaagttcttg
     1321 gagtgctttc agaccgcagt ttttagttat gaagggaaat ggttcgatag tagcttctcc
     1381 ataagtaagc cgactgggta cctcaataaa ttcccaagga tggagataaa aacaaaaaac
     1441 cttaggaagg tttcgcttgg agaaataagt aatcatgttt tcaatatgat tcattaatac
     1501 ctgggatcct tcggtccgaa aaattggcca ctgatcacgg tctcgtccat attcatcgtg
     1561 gctttcaatg gttaaatcag cgaaggttgg aatttctaaa atatttaaat taccaatttc
     1621 ggtccaattc tcccgtgaag gatggtaagg ggtaagctgt ttttggtagt aatacattgg
     1681 gtaagacgag tcggctaaat aacccaaatc atttaacgta ttcaaggtta tttcatttcc
     1741 ccaaaaccgg gtggtacgga aagaaagggg ttgagttcca atgacgtcgc cgactatttc
     1801 agttgctttt tgaattcgat aagcaacctc ttcagagagc actggcttaa tgaggggaat
     1861 atcgaagtat ggctcaccaa gcgtctcatg ctggagagta tgacaaccat tttcatgacc
     1921 acatctttcg atttctttag ccaaggattt aaactgcgat atgatatcgg ctacccataa
     1981 gaaggtcgcc ggaattcctt tcttttcaaa gagttttaaa agcaaagggg ttcccttttc
     2041 aactccttgc aaaaagggag tgaagctgcc gatatcggtc tccatatcaa ttcccaggag
     2101 aacaaaaaca tcattcatga gg
//
LOCUS       DAOSOL010000252         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_52622_length_2108_cov_4.132002, whole genome shotgun sequence.
ACCESSION   DAOSOL010000252 DAOSOL010000000
VERSION     DAOSOL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52622_length_2108_cov_4.132002"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="PK171_05885"
     CDS             complement(<1..1425)
                     /locus_tag="PK171_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT05752.1"
                     /translation="MNQYILVLDCGATTLRAVAVGMKGEILAISALPNAPRLQSGCED
                     CLIWDLDEIWNKFLQIIPKVRSQVNGKVVAVTVTTFGADGTTVKKDGTLTYPVISWQC
                     SRTTEWAKRIASLISPREIFRLTGYQIIPFNTIIKLLWMRENVPEALDEADTYMMMPG
                     LLSYKLCGEMSFDYPSASTTMMIDIRTKKWSERLLSLVGLDETFFPPFLPSGGVIGRI
                     TEKVSSETGIPLGIPVIAAGHDTQFALIGSMADQSEMVLSSGTWEIAAIRILEYRDSE
                     RAFETGIMTEFDAVDGLWDPQMLMMAGGVVEWVRRNFFADIKDRSDIYSLMIDNAREI
                     EPGSGGVTVVPSFMPSGPNKPYETQGTILGLGLTTDRSQVYRAALEGLSFQLKQAVKA
                     FYETFSFEPTAVRVVGGGSKNHLWNQIRADVLGLPVITTSCEEGTVLGAALFAMVGSG
                     IARSIEEAKKMVEISTRVWEPSENW"
     gene            1664..1921
                     /locus_tag="PK171_05890"
     CDS             1664..1921
                     /locus_tag="PK171_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05753.1"
                     /translation="MNKSTMQVRGLIALGMLILIFIMIITGVILWLAMLGVMNHPGLW
                     SAASQIHPNVGIIMFILGMVHFITNKKMFLNDLKQLKGKEY"
BASE COUNT          619 a          458 c          389 g          642 t
ORIGIN      
        1 ccaattttcc gaaggttccc atacccgagt ggatatttcg accatttttt ttgcttcttc
       61 gatggatcga gctataccgg aaccaaccat agcaaagaga gctgctccta aaacggtccc
      121 ttcctcgcaa gaagtagtaa tcaccggaag tcctaaaaca tctgctcgta tttgattcca
      181 aaggtggttt tttgaacctc ctccaaccac ccttactgct gttggttcaa aactaaaggt
      241 ttcataaaat gcttttaccg cttgcttaag ttgaaaggat aatccttcca gagcagctcg
      301 ataaacttgc gaacgatcag tagttagccc tagccctaag atcgttcctt gtgtttcata
      361 aggcttattc ggaccggagg gcataaagga gggaaccact gtaactcccc cgcttccagg
      421 ttcaatctct ctggcattat caatcatcaa tgaatagatg tctgatcgat ctttaatgtc
      481 agcaaaaaaa ttccgtcgaa cccactccac cacaccaccg gccatcatca gcatttgagg
      541 gtcccaaaga ccatctaccg catcaaattc ggtcataata cccgtttcaa aagctctttc
      601 tgagtcacga tattctagga tccgtatcgc agcaatttcc caggttcccg aactcaaaac
      661 catttcactt tggtcagcca ttgacccaat taaagcgaat tgagtatcat gaccagcagc
      721 aataactgga attcctaagg gaatcccggt ttctgatgac actttttccg taatccgccc
      781 gatcaccccc ccactaggaa gaaatggtgg aaaaaaggtt tcatctaatc caactaaaga
      841 taataaacgc tccgaccatt ttttggtacg aatatcaatc atcatggttg ttgaagccga
      901 gggataatca aaagacattt ctccgcaaag cttatagctt aataacccag gcatcatcat
      961 ataggtatcg gcttcatcca atgcttctgg aacattttct ctcatccaca atagcttaat
     1021 aatggtattg aaaggaatta tttgatagcc agtgagtcgg aaaatttctc gtggggatat
     1081 taacgatgca atcctttttg cccattcggt tgttcgagaa cattgccagg atataaccgg
     1141 ataagttaat gtaccatcct tctttaccgt cgttccatca gctccaaagg tggtaacagt
     1201 gactgctact acctttccat tgacttgaga ccttacctta ggaataatct gtaaaaactt
     1261 attccatatt tcatctaaat cccaaatgag acaatcttcg cacccacttt gcaatcgggg
     1321 agcattaggc aaggccgaaa tagccaatat ttctcctttc atcccaactg ctactgcccg
     1381 gagcgttgtt gcgccacaat caagtaccaa aatatattga ttcacagttt tccctccgat
     1441 ttaatttata tttaggtttg atgggaaaat attttcaatg gaaatttttc ctggcttcta
     1501 ggggagattc tttaaaaaac catctgaccg aattgctctc atcctttctc tccattatat
     1561 tataatgaaa taaagaattt ttcttattat aaaatttttc tctttttaaa aaaaggcgtt
     1621 attttctttt ccctaatcca aagctaatag aggaggaaag ataatgaata agtcaactat
     1681 gcaagttcgt ggtttgattg ccttggggat gcttattctc atttttatta tgatcattac
     1741 cggggttatt ttgtggttag ccatgcttgg agttatgaat catccaggat tgtggagcgc
     1801 tgcttcccag attcatccta acgttgggat tatcatgttt atcttaggaa tggttcattt
     1861 tataaccaat aaaaagatgt ttttgaacga tttaaaacaa ttaaaaggga aagagtatta
     1921 aattggtaga ctctcatgaa atgttatggt accaggtgaa aataaaaata cctatcgaaa
     1981 accgtcattg agcggaagca gagttttttt cccttgaggg gagatagtca ggatgtgggt
     2041 gaaacctaag attcagaccc aggtttggtg ttttaaaacg aacttgagag agttttgtag
     2101 gttccccc
//
LOCUS       DAOSOL010000253         2099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_52922_length_2099_cov_4.065068, whole genome shotgun sequence.
ACCESSION   DAOSOL010000253 DAOSOL010000000
VERSION     DAOSOL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52922_length_2099_cov_4.065068"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1077
                     /locus_tag="PK171_05895"
     CDS             274..1077
                     /locus_tag="PK171_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT05754.1"
                     /translation="MKIDVHGHIGSVNLHPNWTANAKQVSEMTQKAGVDHCFVSSAMS
                     IMYDTCKGNDEVLEAVREFDNLWGYMVVNPFFPESFRYLEYIGRERKIVGCKIHPDYH
                     GYDLSSPMGKDLVRRVVDHTSLILFHTSCMAGTGFSRIGTLGELASKYSQATFISAHL
                     AGLYQNPLYPYYINYSGLEEAAQMNLPNLLIDTSSFWIYAYPGVMKRIVELVGPDKLV
                     FGTDIPIQSDMQIRFKIIAIDSLELPQSDKEKIYSKNIRKSLQILKEFK"
     gene            1115..>2099
                     /locus_tag="PK171_05900"
     CDS             1115..>2099
                     /locus_tag="PK171_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05755.1"
                     /translation="MFKLKNLPILLLLSFLILSVFSSQLLAAEKIVINEMMFSGAAQE
                     VLIEQAKRFMEENPDIEVNITWVDYASLHEKMMTELVGGTGRYDVMAAITDFMPEFIG
                     GGYLEPLDSLIENDPPEGWPDDFPDSLLRYQKDADGKIYGLPWWDGPVMFYYRKDLFE
                     NPQEKENFQKEYGYELNPPMTWKEFLDIAQFFTRDTDQDGTVDFYGAVQGARQGGQNL
                     VYDVLLMFFTNGIEILDENFKPVFNTEAGAEAIQFYADLMNKYKVSPQASTTFDVPES
                     GDFFLNGNCAMHWNWAHIAGFAEDPEKSKIVGNCGYSLMPKGENGSNKSLIS"
BASE COUNT          667 a          349 c          422 g          661 t
ORIGIN      
        1 aaaaaattta tttatgctat cattttaacg ttaaaataat ctgaataaga agatatcaat
       61 gagtcatttc caactcaatg atattaggaa cttttttcaa ggtcatgaaa tgagaaatcg
      121 aatagcactg gacgttacaa agagtagcat ttatttcccc ttgatataca accaaaactg
      181 ccaacaacct aaaaaattac atttttagta attgaaaata atcatttatt ttaaaaaaat
      241 aaaatttaga actcaacatc tgaaggtgag aagatgaaaa ttgacgttca tggacatatt
      301 ggttcggtta acttgcaccc caattggaca gccaatgcaa aacaagtcag tgaaatgacc
      361 caaaaagccg gtgtcgatca ttgttttgtc tcttcggcaa tgagcatcat gtatgatacc
      421 tgcaaaggaa acgatgaagt tttagaagca gttcgagaat ttgacaacct ttggggctat
      481 atggtggtca atcctttttt ccccgaatct tttcgttatc ttgaatacat aggaagagag
      541 agaaaaattg taggatgtaa aatacatcct gactatcatg gttatgactt atcttcacca
      601 atgggaaaag atttggtgag aagagtcgtt gatcatacct ccctcatttt gtttcatact
      661 tcctgcatgg ccgggaccgg tttttccagg attggaactc ttggtgagtt ggcttcaaaa
      721 tattcccagg cgacattcat ttctgctcat ttagctggat tgtatcaaaa ccccttgtat
      781 ccttactata taaattattc tgggttagaa gaagcagccc aaatgaatct tccgaattta
      841 ctcattgata cctccagttt ttggatttat gcctatccgg gtgtaatgaa aagaattgtt
      901 gagcttgttg gtccagataa gttagttttt ggtacagata ttcctattca atcggatatg
      961 caaatccgct ttaaaattat tgccatcgat agtttagagc ttccccaaag cgataaagaa
     1021 aaaatatatt ctaaaaatat tcggaaatct cttcagatat taaaagagtt taaatagtat
     1081 taatttttaa ttctaataat aaggaggaaa gaaaatgttc aaattaaaaa acttaccaat
     1141 tttattactt ttaagttttt tgatactcag tgttttctct tcccaacttt tggctgcgga
     1201 gaaaattgtg atcaatgaaa tgatgttttc cggtgctgcc caggaagtat taattgagca
     1261 ggcaaaacgg tttatggaag aaaatccgga tattgaagtt aacatcacct gggttgatta
     1321 tgcttcgctg catgaaaaaa tgatgacgga attggttggt ggtaccggta ggtatgacgt
     1381 gatggctgct ataaccgact ttatgccgga atttattggt ggtggctatc ttgaaccact
     1441 cgatagcctg attgaaaacg atcctcctga aggatggcca gatgattttc ctgattcatt
     1501 attgagatat caaaaagatg ctgatggaaa aatttatggt ttgccctggt gggatggacc
     1561 agttatgttt tattatcgaa aagatctctt tgaaaacccg caagagaaag aaaatttcca
     1621 aaaggaatat ggttatgagc tcaatccacc aatgacctgg aaagagtttt tagatattgc
     1681 tcagttcttt actcgagata ccgatcaaga tgggacggtt gatttttatg gagcggtgca
     1741 aggtgcccgt cagggaggac agaatttagt ctatgatgtc ctgttgatgt ttttcaccaa
     1801 tgggattgaa atccttgatg aaaacttcaa accggtattt aatactgaag ctggagctga
     1861 agccatacaa ttctacgctg atttaatgaa taaatataag gtctctcctc aagcatcgac
     1921 aacttttgat gttccagaaa gtggcgattt ctttttaaat ggaaattgtg ctatgcactg
     1981 gaactgggct catatcgctg gttttgctga agaccccgag aaatcaaaga ttgttgggaa
     2041 ttgtggctat agcctcatgc ctaagggaga aaatgggagc aacaaatctc tcatttcct
//
LOCUS       DAOSOL010000254         2087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_53335_length_2087_cov_3.749508, whole genome shotgun sequence.
ACCESSION   DAOSOL010000254 DAOSOL010000000
VERSION     DAOSOL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53335_length_2087_cov_3.749508"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PK171_05905"
     CDS             <1..933
                     /locus_tag="PK171_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT05756.1"
                     /translation="QPLLKKLDTFPNLKIVSSLVEGSGFITKAEVSRPVVVKGIFLDR
                     ADGIYKIIQRVNNGKAVLGGTGVLIGRGLAEDLKIETGSIIEIRTSEGNSGAFAVNGI
                     FDFENAAINSSWIFMDIFRSQALFDLNGAISRIEMQIDQVFQSENIAQKIKKDNPDFL
                     IETWQSNNRQLLSALQSQSASSYMIQFFVLLAVTLGIASVLAIAAVQKSREIGILKAM
                     GTRTNSASRIFLIQGAVLGLSGSILGSLVGVGLIKMFQIASQSGGALSFSIQVEFRTA
                     ISLIVISTIASIGAALFPARKAASLVPIEVIRNG"
     gene            926..1684
                     /locus_tag="PK171_05910"
     CDS             926..1684
                     /locus_tag="PK171_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05757.1"
                     /translation="MDNILVSLDSVNKVYGDEVKTQVLFDVSLEIPQGQFLALIGPSG
                     SGKSTLLNILGALDRPSSGTILINQQNLVQLNENQLADFRNKFLGFVFQFHYLLPEFT
                     TIENVLLPYWIGKGKPNRQMFNEAQRLIERVGLANQINKRINLLSGGQQQRVAIARAL
                     LNQPQLILADEPTGALDTKSGEQVLDLMQEINGENNTTFIVVTHDRNVALRAERIIEL
                     IDGRICKDFFPQIVGKEKSIEILESHACVWDDPS"
     gene            complement(1715..>2087)
                     /locus_tag="PK171_05915"
     CDS             complement(1715..>2087)
                     /locus_tag="PK171_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05758.1"
                     /translation="IVLVKNDLNDVVTALELSEVTMGKIKQNLFFALFYNLIGIPIAA
                     RVFASFGLLLNPELAGLAMALSSISVVTNSLLLRTFRPRKRNYLSMVAPIIMILLFTY
                     IFTLFARFSTTMSNMMTTIPK"
BASE COUNT          628 a          372 c          459 g          628 t
ORIGIN      
        1 cagccattat taaaaaaatt ggataccttt cccaatttaa agattgtatc ttccctggtt
       61 gaaggatcag gatttattac caaagctgaa gtaagccgac cagtagtggt taaaggaatt
      121 tttcttgatc gtgctgatgg tatttataaa attattcagc gagtgaataa tggaaaagcg
      181 gttttgggag gaaccggggt actaattggg aggggtttgg ctgaagacct caagattgaa
      241 accggtagta ttattgaaat tcgaacctct gaaggtaact caggggcctt tgccgtcaat
      301 gggatttttg attttgaaaa cgcagccata aacagttcct ggatttttat ggatattttt
      361 cgatcccaag ccttgtttga ccttaatgga gcgatatctc gtattgaaat gcaaatcgat
      421 caagtattcc aatcggaaaa cattgctcaa aaaattaaaa aagataatcc tgatttcctc
      481 attgagacct ggcaatctaa caaccgccaa cttttaagcg ccttacaaag tcaaagtgcc
      541 tcttcctata tgattcaatt ttttgtttta ttggcggtaa ctttaggtat cgccagtgtt
      601 ttagccatag cagcggttca gaaatcacga gaaattggaa ttttaaaagc catggggact
      661 cgaaccaata gcgccagtcg tatcttcctt attcagggag cggttttagg attgagcggt
      721 tctattttgg gaagtttggt tggagtgggt ttaattaaaa tgtttcagat cgcatcacaa
      781 tcgggaggag cactttcttt tagtatccag gttgaatttc gaaccgccat aagcttgatt
      841 gttatttcaa ccattgccag tattggtgca gcacttttcc cagctcggaa agctgcttcc
      901 ttagtaccaa ttgaggtaat tcgaaatgga taatatatta gtgagcctgg actcggtgaa
      961 taaagtttat ggtgatgagg tgaaaaccca ggttcttttt gatgtttctc ttgaaatccc
     1021 ccaaggccag tttttagcgt taattggccc ttcaggatcg ggtaaaagta ctttattaaa
     1081 catactcggg gctcttgatc gcccgtcgag cggcactatc ttgatcaatc agcaaaattt
     1141 ggttcaactc aatgaaaatc agttagctga tttcaggaat aaatttttag gtttcgtttt
     1201 ccaatttcac tatcttcttc ccgaatttac taccattgaa aatgttttgt tgccttattg
     1261 gatcgggaaa gggaaaccga atcgccaaat gtttaatgag gctcaaagat tgattgagcg
     1321 agtagggtta gctaaccaaa ttaataaacg gattaacctt ctttccggtg gtcagcagca
     1381 gcgggttgct attgcccggg cgctcctaaa ccaacctcag ctcattttgg ccgatgaacc
     1441 aactggtgct ttagatacca agtcgggaga gcaggtattg gatcttatgc aggaaatcaa
     1501 tggtgaaaat aacaccactt ttatcgtggt gactcatgat cgaaatgttg ccctgcgggc
     1561 ggaaagaatc atcgaactca tcgatggtcg gatttgtaaa gatttctttc cgcaaatagt
     1621 agggaaagaa aaatcaattg aaatcttaga atcccatgct tgcgtgtggg atgatccatc
     1681 ttaaaaatta actttggaga atactttaaa tttgttattt gggtatggtt gtcatcatat
     1741 tactcatggt ggtgctaaag cgagcgaata aagtaaatat ataggtaaat aaaagaatca
     1801 taataattgg agcaaccatt gaaagatagt ttcgttttcg cggccgaaaa gttctcagga
     1861 gcaaagaatt ggtaacgact gaaattgaac ttaaagccat ggctaaacca gctaattcag
     1921 gatttaaaag cagaccaaag gaggcaaaga ctcgagcggc aataggtatt ccaatcaggt
     1981 tataaaaaag agcgaaaaag aggttttgct taattttccc catagttact tctgatagct
     2041 ccagagcggt aaccacatcg ttgaggtcat ttttaaccaa gacgata
//
LOCUS       DAOSOL010000255         2080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_53581_length_2080_cov_4.103210, whole genome shotgun sequence.
ACCESSION   DAOSOL010000255 DAOSOL010000000
VERSION     DAOSOL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53581_length_2080_cov_4.103210"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PK171_05920"
     CDS             <1..895
                     /locus_tag="PK171_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOT05759.1"
                     /translation="PQNQSKFKRKNEDVKRENKIDRGTERKINGVDSNELFYSIETIK
                     IFNADILKMKSVKDNSVDLIVTSPPYNVDIKYNSHKDAMTYEDYLSFTKRWIERCYKF
                     VKEDGRFCLNIPLDKNKGGQQSVCADMTTLAKQIGFKYHSTIIWNEQNISRRTAWGSW
                     LSASAPYVIAPVEVIIVLYKNSWKKTSGSKQSDITKEEFMEWTNGVWNFSGESKKRIG
                     HPAPFPVELPRRCIKLFSFVGDTVLDPFMGSGSTLIACCETKRLGIGVEIDQAYCELA
                     RKRLKKECQLDQLKINFSGGE"
     gene            899..1486
                     /locus_tag="PK171_05925"
     CDS             899..1486
                     /locus_tag="PK171_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOT05760.1"
                     /translation="MSRTIRSLILEFFYQHPKQDLEHGPVVDWVTEQHLKENTTPPRD
                     IWRTVRHLHQEGILIKVNKGVYRYDPEQVTNRELFDFSEETKEAILQRDQYQCVVCGR
                     GRKDGVELCVDHIKPKDKGGDNSIENGQTLCTEHNLLKKNYTQTEAGKRYFIKIYKQA
                     VANNDIRMINFCKGIFDVYDTYKLNGHIKRPNGKE"
     gene            complement(1515..>2080)
                     /locus_tag="PK171_05930"
     CDS             complement(1515..>2080)
                     /locus_tag="PK171_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOT05761.1"
                     /translation="RKIPEVLTAEEQRAFLRTFNRKAPTGLRNYAMMRLMLDAGLRCG
                     EVLNLRSKDIDWKSGKIHIHQGKGKKDRIVWLNDDALGVLRQWKEIKPESDLLFTTLR
                     GDKMNDRYVRALVERKAKQAGIEKDVHPHTLRHTFATDIYRESKNLRLVQKALGHSSI
                     QITEIYTHILDEELEEGMRFFRKVSIA"
BASE COUNT          677 a          406 c          424 g          573 t
ORIGIN      
        1 tcctcaaaac caaagtaaat ttaaacgtaa aaatgaagat gttaaaagag aaaataaaat
       61 tgatcgtggc acagaaagaa aaataaatgg ggtagattca aatgagttat tttattctat
      121 agaaacaatc aaaattttta atgcagacat tttaaaaatg aaaagcgtta aagacaattc
      181 tgttgattta attgttacat cgccacctta taatgtagac ataaaatata actcacataa
      241 agatgcaatg acttatgaag actatctttc atttacaaaa agatggattg aaagatgcta
      301 taagtttgtt aaagaagacg gaagattttg cctaaatatt cctttagata aaaataaggg
      361 tggtcaacag agcgtatgtg ccgacatgac cactttagca aaacagattg gttttaaata
      421 tcattcaaca attatttgga acgaacaaaa tatttcaaga agaaccgctt ggggatcgtg
      481 gctgtcagct tcagctcctt acgttattgc tccagttgaa gtgatcatag ttctctataa
      541 aaacagctgg aagaaaacca gtggaagtaa gcagtctgac atcaccaaag aggagttcat
      601 ggagtggacc aatggggtgt ggaacttcag cggtgagagc aagaagagga tcggacatcc
      661 tgctccgttc ccagtcgaac tcccccgacg atgtatcaag ctcttcagct ttgttggtga
      721 cacggttctt gatcccttca tgggaagtgg ttcgacctta attgcttgct gtgaaactaa
      781 gcggttggga attggtgttg aaattgatca agcctactgt gagctggcac ggaaaagact
      841 caagaaagaa tgccagcttg accagttaaa aattaacttc tctggaggag aataataaat
      901 gagtagaacc attcgaagtt tgatattgga gttcttttat cagcatccca agcaagactt
      961 ggaacatggt cctgtggtag attgggtgac agaacaacac ctgaaagaaa atacaactcc
     1021 accacgtgac atttggcgaa cggttcgcca ccttcatcaa gaagggatac tcatcaaagt
     1081 caacaagggt gtctatcgat atgaccccga gcaagttacc aatcgagagc tctttgattt
     1141 ctcagaagaa actaaagaag caattctcca gcgagaccaa taccagtgtg tggtttgtgg
     1201 aagagggaga aaggatggag tagaactctg tgtcgatcac attaaaccta aagataaggg
     1261 aggcgataac tcgatcgaga atggacagac gttgtgcact gaacacaatc tcctgaagaa
     1321 gaattatacc caaaccgaag caggcaaacg ttacttcatt aaaatctaca aacaagccgt
     1381 agccaacaac gatatcagaa tgatcaactt ctgtaagggt atttttgatg tttatgatac
     1441 ttacaagtta aatggtcata taaaaaggcc aaatggaaag gaataaaaac caatttttgt
     1501 gataaattta tccttcatgc aatcgagact ttccgaaaaa acctcatacc ctcttctaat
     1561 tcctcatcaa gaatatgagt ataaatttcg gtgatctgaa ttgaggaatg gccgagggct
     1621 ttttgaacta accgtaaatt tttgctttcc cggtaaatat cagttgcaaa ggtatggcga
     1681 agagtgtgag ggtgaacgtc tttttcaatt ccagcttgtt tagcctttct ttctactaac
     1741 gcccggacat atcgatcatt cattttatct cccctcagcg tggtaaacag aagatcgctt
     1801 tccggtttta tctctttcca ttgtcttagg acccctaagg cgtcatcatt tagccaaacg
     1861 atccggtcct tttttccctt cccctggtga atatggatct ttccgctttt ccagtcaata
     1921 tctttggacc gtaaatttaa cacttcacca caacgcaaac cagcatctaa catcaacctc
     1981 atcatggcgt aattccgtaa accagtcggg gccttccggt tgaaggtccg caaaaaggcc
     2041 cgttgttcct ccgcggttaa aacttccggg atttttcttt
//
LOCUS       DAOSOL010000256         2078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_53664_length_2078_cov_3.114681, whole genome shotgun sequence.
ACCESSION   DAOSOL010000256 DAOSOL010000000
VERSION     DAOSOL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53664_length_2078_cov_3.114681"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..750)
                     /locus_tag="PK171_05935"
     CDS             complement(142..750)
                     /locus_tag="PK171_05935"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOT05762.1"
                     /translation="MKRRKKRLAFLQPILYILIGMAVFFAIVYAGEYLIPGGKKNTVA
                     PDDTGFLSTGQTSSAISGDSTVEVVLYFTDEDFSGLVAEKRLIEKHHNMLESVLQELL
                     RGPKLSSHYNPLPESTRLNGVFTESGIVYVDLSHEMKDGQSGGTTQELLSIFSIVDTL
                     TSLPDVKRIKILIDGKEETTLCGHIDISEPLERDEKLIAEIQ"
     gene            complement(757..>2078)
                     /locus_tag="PK171_05940"
     CDS             complement(757..>2078)
                     /locus_tag="PK171_05940"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT05763.1"
                     /translation="VYISPQFLNQLFGISTDTSSTVVTPSPSPTKTPSTISPVTLPTG
                     SSSLLNVRSHSYGDENRSRVTFDFGINLPTHSMQTDQANNRIILTFNNATLAPGTPET
                     FSLNDNRVDRVVLNKKGSNVEAIIYLKSPASIQKNQLGGENPRIYLDIVTSTLLTSPD
                     VTILPASPSPPTQPTTTTPALTSTPTPVVPTPTTKPPVQPTTATPYDKINPRVIVIDP
                     GHGGKDPGCVVNGYQEKDIILQVSKKLKEALTQQGYEVYLTREGDTYPGLKERSAVAN
                     NKQPVVLLSIHANAAPNKKATGVEVFVGSAQIHGEGAADVANRENQRFISEGYTEEQN
                     GKINSTLLDSYYLGSRTVSMKLGSLIENNIVKETGQASRGLKEAPLILLKGMYFPSCL
                     IEIGFLSNPTEAKNLANGAFQDKLVRGIVVGIKQFMSSSDLKKFLEE"
BASE COUNT          584 a          387 c          448 g          659 t
ORIGIN      
        1 tcaattaaaa ctgaaccttc agctgctcta agataatttc tggtgatcat gagagaacgt
       61 atctcatcgt agtttcgacc atcgtttctt aagtggatat catcccgttc tttctttaaa
      121 aaagaattca aatcctgaaa actattgaat ttctgcaatg agtttttcat ccctttccag
      181 tggttctgat atgtcgatat gtccacaaag agtagtttct tcctttccat caattaatat
      241 tttaatgcgt ttaacatcag gaagtgaggt taaagtatct acaatactaa aaatactgag
      301 aagttcttgg gtggttccac ccgattgccc gtctttcatt tcatggctga ggtcaacata
      361 aacaataccg ctttcagtga aaacaccgtt taatcgcgtt gattcaggga gtggattata
      421 atgagatgaa agcttgggtc ctctcagcag ttcttgaaga accgactcaa gcatattatg
      481 atgcttttca attagtcgtt tttctgcaac taagccacta aaatcctcat cagtaaagta
      541 aagaacaact tcaactgtag aatcaccact gatcgctgaa gaagtttgac cggtcgagag
      601 aaatcccgta tcatcaggag ctacagtgtt cttttttccc ccggggatca agtattcacc
      661 agcgtagacg attgcaaaaa aaactgccat tccgataagg atatataata ttggctgaag
      721 aaaagcgagt cttttttttc ttcttttcat tcggtcctat tcctcaagga atttttttaa
      781 atctgaactt gacataaact gttttatgcc aaccactatt cctcgcacca gtttgtcctg
      841 aaaggctccg tttgctaagt tttttgcttc ggttgggttg ctcaaaaaac caatttcaat
      901 caaacatgag gggaaataca ttccttttaa tagaataagg ggtgcttctt taagccctct
      961 tgaagcctgg ccggtctctt tgacgatgtt attttcgatt aaagaaccga gcttcatgct
     1021 cacggtacga cttcctaaat aataagaatc aagaagagta gagttaattt ttccattttg
     1081 ttcttcagta tacccttctg aaataaaacg ttgattttca cgatttgcaa cgtcagcggc
     1141 cccttcgcca tgaatctgtg cacttcctac gaaaacttca accccagtag cttttttatt
     1201 aggggcagca ttggcatgaa tgcttaataa aaccactggc tgtttattat tggctactgc
     1261 ggatcgttct ttaaggccag gataggtatc gccttcacgg gtcaaatata cttcatagcc
     1321 ttgttgagta agggcttctt taagcttttt agaaacctgg aggataatgt ctttttcttg
     1381 atacccgtta acaacacaac cggggtcttt cccaccatga ccaggatcga taacaataac
     1441 cctgggatta attttgtcgt aaggtgttgc agtcgttggt tggactggtg gtttggttgt
     1501 aggcgtagga acaacaggtg taggagtaga cgtaagcgct ggtgttgtag tagtgggttg
     1561 tgttggtgga gatggacttg ctggaagaat tgttacatca ggagatgtta acaaagtgga
     1621 agtaactatg tctaaataaa tacggggatt ctctccgccc aattggtttt tttgaattga
     1681 ggcgggagat tttaaataaa taatagcttc gacgttactc ccttttttat taagcactac
     1741 tcgatctact cgattatcgt ttagagagaa cgtctctggt gttcctggag ccaaggtagc
     1801 attgttaaag gttagaataa tgcggttgtt ggcttggtcg gtttgcatgg aatgagttgg
     1861 taagttaatc ccaaaatcaa aagtaaccct tgagcgattt tcatcaccat aggaatgaga
     1921 ccgaacatta aggagtgacg agcttccagt aggaagggta accggtgaga ttgttgaagg
     1981 tgtttttgtt ggtgaaggtg acggcgttac gactgttgaa ctggtatcag tggaaattcc
     2041 aaagagctga ttaagaaatt gaggtgaaat ataaacgg
//
LOCUS       DAOSOL010000257         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_53695_length_2077_cov_3.123145, whole genome shotgun sequence.
ACCESSION   DAOSOL010000257 DAOSOL010000000
VERSION     DAOSOL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53695_length_2077_cov_3.123145"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PK171_05945"
     CDS             complement(<1..916)
                     /locus_tag="PK171_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05764.1"
                     /translation="MEKRPTFREKVLAVFQGKNTNNIPWQPRLEHWFNVNRARNQLVE
                     PYQSMKLLDLYKKLNCSVRYYYGEAQDISSPNTFIHFDYPPGAGVFEFQQGDDIKVIF
                     RSAKGEELIGKKRLGEWGCSWHYIEHPVKNIEDLRVLEDIVTSVSYRFDKDFYLEAKK
                     AMGEWGEIQFYWERSPFQRLFLQYAGIENTIQLIYDYPSRLQEYLKKAEEAEDSLFEI
                     LASCPVKILNFGENIDGRFNSPRIFNQFLVPYYQKRVQQLHKSGKFCHIHMDGSLKPL
                     LPYLRDSGFDGIEGATPQPQGDVSLEELK"
     gene            complement(923..>2077)
                     /locus_tag="PK171_05950"
     CDS             complement(923..>2077)
                     /locus_tag="PK171_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOT05765.1"
                     /translation="LIRKINRSNIIGLIRKQGSLSRSEISRISGLSLPSVSRIIDSLI
                     QEGILKEIGKGESLRGKKPLLIDLNSDHQYVFGVEISRKGQILLCNLTGTVLKREQYF
                     PDPSQGPLSIASQLSEKISMLCHLHNLLPNKIAGVGIGTPGFLFKAGPLINQSPFFGW
                     NNVNAFQVFENSFPYPVLVENVAKASAVAEKMYGYGKQFDNFFYVFTDWGVGGGFVTS
                     GSLYRGLNGNAGEFGHTIIQQNGVPCYCGNLGCLEQYTSTDAIVREAKAINKNFKDFD
                     SVMIAYQLGDTVIASLLKKSGEILGKGVANIINLLNPEAIIIGGEIARKCPIYIQSAI
                     EKAHDAVFSLEAQKTPIFTSQLGEDAVATGMASEIISNFINENLTMKRGG"
BASE COUNT          629 a          436 c          394 g          618 t
ORIGIN      
        1 cttttaactc ttctaaagaa acatctcctt gtggttgagg ggtagcaccc tcaatcccat
       61 caaatcccga atctcggagg tagggcaata ggggtttgag tgaaccatcc atatgaatat
      121 gacaaaactt acctgattta tgtaactgtt gaactcgttt ctggtaataa gggacaagaa
      181 actgattaaa aatacgagga gaattaaacc ggccatcaat attttcacca aaatttagaa
      241 tttttactgg acaggaagct aaaatttcaa atagtgaatc ctccgcttcc tcggcttttt
      301 tcaaatactc ttgaagccgg ctaggatagt catagatgag ctggatagta ttttcaatac
      361 cagcatattg taagaagagc cgttggaaag gtgatctttc ccaataaaac tgaatctctc
      421 cccattcacc catagctttt tttgcttcca agtaaaaatc cttatcgaaa cgataactaa
      481 ccgaagtaac aatatcttcc aaaactctaa ggtcttcaat gtttttaact ggatgttcaa
      541 tataatgcca ggaacatccc cattctccca agcgtttttt tccgatcagc tcttcaccct
      601 tggctgaccg aaaaatcacc ttaatatcat ctccctgttg gaattcaaaa accccagcac
      661 caggaggata atcaaaatga atgaaggtat tcggtgaaga aatgtcttgg gcctcgccat
      721 aatagtaccg taccgagcag tttaattttt tatataagtc gagcagcttc atcgattggt
      781 aaggttcgac aagctggttt cgtgcgcggt tgacattaaa ccaatgttcc aagcgtggtt
      841 gccaaggaat attgttagtg tttttacctt gaaaaactgc aagaactttt tctcggaatg
      901 taggtcgttt ctccatcgtt tgctaccccc ctctcttcat ggtcagattc tcgttaataa
      961 agttactgat tatttcactg gccattcccg tggcaacagc atcttctcca agctggctgg
     1021 tgaaaatagg tgttttttga gcttctaaag aaaagacagc atcatgagct ttctctattg
     1081 ccgattggat gtaaattggg catttacggg caatttctcc gccaataatt atggcttctg
     1141 gattgagtag gttaataata ttagctactc ctttccctaa aatctctccg gattttttga
     1201 gcagagaagc aataactgta tcaccgagtt gataggcaat catgaccgag tcaaaatctt
     1261 taaaattttt attaatggct tttgcttctc gaacaatggc atcagtagag gtgtattgtt
     1321 cgagacagcc aaggttcccg caataacaag gaactccatt ctgctgaatg atagtatgac
     1381 caaattctcc ggcattcccg tttaatcctc gatataatga accgctggta acaaaccctc
     1441 cccctacacc ccagtcggta aagacataaa aaaagttgtc aaattgtttt ccgtaaccat
     1501 acattttttc cgcaacagcc gaagccttcg ctacgttttc aactaatact ggatagggaa
     1561 aggaattctc aaatacttga aaagcattga cgttattcca gccaaaaaat ggcgattgat
     1621 taatgagagg tccagctttg aagaggaatc ctggggttcc aataccgacg ccagcaattt
     1681 tgtttggtaa caaattatgt aaatgacaaa gcatggagat tttttccgaa agctgactag
     1741 caatagacaa aggaccttgg gaaggatcag gaaaatattg ttcacgtttt aaaaccgtac
     1801 cggttaaatt gcaaagtaga atttgtcctt ttcgggaaat ctctactcca aagacatatt
     1861 gatggtcact gttgaggtct atcaagagtg gcttcttccc ccgtaacgac tcgccttttc
     1921 caatttcttt taaaatgccc tcttgaatta aggagtcaat aattcgggaa accgatggta
     1981 aggaaagacc gctgattcgt gaaatctccg atcgagaaag cgatccctgt ttccgaatca
     2041 atccaataat attagaacga ttaattttcc gaattaa
//
LOCUS       DAOSOL010000258         2071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_53920_length_2071_cov_3.639385, whole genome shotgun sequence.
ACCESSION   DAOSOL010000258 DAOSOL010000000
VERSION     DAOSOL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53920_length_2071_cov_3.639385"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PK171_05955"
     CDS             complement(<1..476)
                     /locus_tag="PK171_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05766.1"
                     /translation="MQTNPNVKSPGVGSGTLVKNNKYQLIFRSQWFLLLLAEFLIAII
                     TGIVNPRFFTVSNIINVLEQIAVLGIVSSGMTLLIISGEIDISVGANIGLSSCVMAIM
                     IKSGIGVFPSFVTGIALAIFSSFLVGLTAHTFKAPSFITSLAFISVFQGIALAITKG"
     gene            complement(460..1989)
                     /locus_tag="PK171_05960"
     CDS             complement(460..1989)
                     /locus_tag="PK171_05960"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05767.1"
                     /translation="MENNQLLVSLQNITKIYGIHKVLDNVSFDLRKGEIHCLVGENGA
                     GKSTLIKILSGAISPESGKLYIAGKKITSMTPRKAIELGISTVYQDAELVESLTVTDN
                     IFLGDEKSASLPFIIDKKIQFQKAQEIINTLHMNLPVDSLVEELSVSQKQMLEIVKAL
                     YRDSKIIIMDEPTSSLGLEEKKALMKIIQNLKQRGIGIIYISHYLEEIFMIGDRVTIL
                     KDGKLVNTYDVASVDIETVIRGMVGREASAFYHRKKIPLGPIQVQIQNLSKKGVLHPV
                     NFDIKKGEILGIGGLVGAGRSELVGLIFGIQQPDSGKVIINGKEAVIHNPRDAIRAGI
                     GLITEDRRKLGLFIGRNLIENMALVHNDVFKGFIVDRDEEKELSNLMVEELSIATSDI
                     NQLVEELSGGNQQKVVIARWLLDDATLCIFDEPTKGVDIGAKQQIYELMVELAEKGKS
                     IIMVSSDMPELLSLSDRIVVMRDNRVVEILDNQQIKEEDLIKKFMGVEEKGEKTNADK
                     S"
BASE COUNT          620 a          432 c          381 g          638 t
ORIGIN      
        1 cctttggtga tggctaaagc aattccctgg aaaacgctga taaatgccag tgaagtaatg
       61 aaggaagggg ctttaaaggt atgagcagtc aacccaacta aaaaactact aaaaattgcc
      121 aaggcaatac cggtgacaaa tgaagggaag acacctatcc cgctcttaat cataattgcc
      181 attacacagg aagataatcc aatattagcc ccaacagaaa tatcaatttc accagaaata
      241 atcaataatg tcattcctga tgagactatt ccaaggacgg ctatttgttc taaaacattt
      301 attatgttgc tcacagtaaa aaacctagga ttgacgattc cagtgataat agcgatgaga
      361 aattcagcca acaaaaggag aaaccactgg ctacgaaata tcaattgata cttgttattt
      421 ttaaccaatg ttccacttcc aacgccaggt gatttcacat taggatttgt ctgcattggt
      481 cttttctccc ttttcctcga ctcccatgaa tttttttatt aaatcttctt ctttaatttg
      541 ctgattatca aggatttcca caactcgatt atcacgcatg acaacaatcc gatcgcttaa
      601 agaaagcagt tctggcatat cagaggagac cataataata ctctttcctt tttcagccaa
      661 ttccaccatg agttcataga tttgctgctt ggcgccaata tctactccct tggtgggttc
      721 atcaaagata cacagcgtag cgtcatcgag aagccatcgg gcaataacta ccttttgttg
      781 attgcctccc gatagttcct ccaccagttg attgatatca ctggttgcga tggatagctc
      841 ttccaccatt agattcgaga gttctttttc ttcatcgcga tcgacgataa agcccttaaa
      901 aacatcatta tggactaagg ccatgttttc aatgaggttt ctgccaatga aaagaccaag
      961 cttgcgacga tcttcggtaa tcaggccaat gccagctcga atggcatccc ggggattatg
     1021 aataacggct tcttttccat tgatgataac cttgccgcta tcaggttgtt gaatcccaaa
     1081 aatcagcccc accaattctg aacgaccagc accaaccaaa ccaccgattc ctaagatttc
     1141 tccttttttt atatcgaaat taactgggtg gaggacacct tttttggata ggttttgaat
     1201 ttggacttgt attgggccca gaggtatttt tttcctatga taaaaagccg aggcctctcg
     1261 acctaccatg ccccgaatca ctgtttctat atcaaccgaa gccacatcat aagtgttaac
     1321 taactttcca tcttttaaaa tagttactcg atcacctatc ataaatattt cttctaaata
     1381 atgagatata taaataatac cgatgccgcg ttgtttaagg ttctgaatga tcttcatcaa
     1441 ggcttttttt tcttctaaac cgagtgagct ggtcggttca tccatgatga taattttaga
     1501 atcacgatag agagctttga caatttccag catctgtttt tgggaaacgg aaagttcctc
     1561 cactaaagag tcaaccggta gattcatgtg gagggtgttg attatttctt gggctttttg
     1621 aaattggatt ttcttatcaa taataaaggg gagagaagca gatttttcgt caccaaggaa
     1681 aatattatcg gtaacggtga gagactcaac caactcggca tcttgataga cggtagatat
     1741 tcccaattca atggcttttc taggagtcat cgatgtgatt tttttaccgg caatgtaaag
     1801 cttaccactt tcgggagaga tggctcctga aaggattttg atgagagttg attttccggc
     1861 accattttca ccaaccagac aatgaatctc gccctttctc aggtcgaaag agacattatc
     1921 aagaacttta tgaattccat aaattttggt gatattttgg agagaaacta aaagttggtt
     1981 attttccatt tttcatcggg ttttaaaaaa gaatgcaaca gggattcccc cgttgcattc
     2041 ttctatagta attttaataa acttcaaggt c
//
LOCUS       DAOSOL010000259         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_53981_length_2069_cov_3.551639, whole genome shotgun sequence.
ACCESSION   DAOSOL010000259 DAOSOL010000000
VERSION     DAOSOL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53981_length_2069_cov_3.551639"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PK171_05965"
     CDS             <1..1044
                     /locus_tag="PK171_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT05768.1"
                     /translation="ESLILFPLLMWGERADFMRSFYIFMNLFLPLFLLDKTIKRRDLV
                     KTGFWIALANILMVIIFGLQEGNAPLEFLVNSVYGFGGAIIASIAALGGISLFETTFH
                     VATDFRLLELVNPTHPLLKRLMMEAPGTYSHSLMMANLAEAAAEAIGANPLLARAGAY
                     YHDIGKIKRPYFFIENQMSENIHNRLSPHLSTLVIQNHLKDGLEIARKYHLPVEIQNI
                     IQSHHGNTVMRYFYDKAIKQKKDDVTDTEFRYPGPLPKTQEEVLIFLADSVEAAIRSA
                     NNLNSSRLEAIVNGIVQNYLKDGQLDDSPLTMRDIHKISDAFVMILSGMFHARVPYPE
                     NTVNGEDKGSKGD"
     gene            1210..2040
                     /gene="cdd"
                     /locus_tag="PK171_05970"
     CDS             1210..2040
                     /gene="cdd"
                     /locus_tag="PK171_05970"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT05769.1"
                     /translation="MMATVIVNRSSKEATLSRAVIKKIVDYVCELENREILGKLSIAF
                     VSLDEIRALKKEFFHQNIDTDVISFFYGTEEPDEVWGEIIICPEKAREYANTYGTKYE
                     NEQKLLLIHGLLHILGYDDIDAKKKHIMETRQNYILEQFLTAEKRDLLVISAQKAQHY
                     AYAPYSLFPVGAALETCDGKVIQGGNIENASLGLSVCAERVALFKAVSMGFATFTRLA
                     VISSEQDYCLPCGACRQVLHEFSPHLEILAARQEGDYQVYILDELLPNPFQFNPEKGR
                     "
BASE COUNT          617 a          397 c          436 g          619 t
ORIGIN      
        1 gaatcgctca ttctgtttcc tcttttaatg tggggcgaaa gggctgattt tatgcggagc
       61 ttttatatct ttatgaatct ttttttgccg ctgtttttgt tggacaaaac tattaaacgc
      121 cgcgatctgg taaaaaccgg tttttggata gccctggcaa acatactgat ggtgataata
      181 tttggtttgc aggagggaaa tgctccttta gaattcctcg tcaattcggt ttatggattt
      241 ggaggagcta tcattgcttc aatcgcagcc ttgggtggga tatccttgtt tgagaccacc
      301 tttcatgtcg ctacggattt tcgccttttg gaattggtca accccaccca tccattactt
      361 aaacgcttga tgatggaggc tcctggaact tatagccaca gtcttatgat ggccaatctt
      421 gctgaagctg ctgccgaagc tatcggagca aatccactat tggctcgagc cggtgcctat
      481 taccatgata ttggtaaaat caagcgaccc tattttttca tcgaaaatca aatgagtgaa
      541 aatatccata atcgcctttc tccccatcta agtacgctgg tgattcaaaa ccatctcaaa
      601 gatggattgg aaatagctcg gaaataccat ttaccagttg aaattcaaaa cattattcaa
      661 agccaccacg gaaataccgt aatgcggtat ttttatgata aagcaatcaa acaaaagaaa
      721 gatgatgtaa ctgataccga atttcgttat ccaggacctc ttcccaaaac ccaggaagaa
      781 gttctcattt ttttagccga tagcgttgaa gctgctatcc ggagtgcaaa taatctcaat
      841 tctagtcggt tggaagcaat agtaaatgga atagttcaaa attatctcaa agatgggcaa
      901 ctggatgatt caccgcttac catgcgggat attcataaaa tttctgatgc ctttgttatg
      961 atattaagtg ggatgttcca tgccagagtt ccttatccgg aaaatacggt gaatggtgag
     1021 gacaagggca gtaagggaga ttaatttcta ttctagaaat gcagttactt gttaaaggct
     1081 aagaatttag aataactgac ctggatatca attgggactt tcacccgcat cctcaacttc
     1141 tcccatcaag tgagaaggaa gtagaatgag taaaggcaat catacctatc gataaattaa
     1201 aaggagtcga tgatggctac tgttatcgtg aaccgttcat caaaagaagc tactctctct
     1261 cgtgcagtaa taaaaaaaat cgtcgattat gtttgtgaat tggagaatcg tgagattctt
     1321 ggaaagttga gcatcgcctt tgtaagtctt gatgagataa gggctttgaa aaaagaattt
     1381 tttcatcaga atatcgatac cgatgtcata tctttttttt atggaacgga agaaccggac
     1441 gaagtatggg gagaaatcat tatttgtccg gaaaaagcaa gagaatatgc taacacctat
     1501 gggactaaat atgaaaatga acaaaagtta cttctcattc atggtctttt acatatattg
     1561 ggttacgatg acattgacgc gaaaaaaaaa catatcatgg aaactagaca aaactatatt
     1621 cttgagcaat ttcttactgc agaaaaacgc gatctattag taatatcagc ccaaaaagca
     1681 caacattatg cctatgcccc ttattcgctc ttcccggtag gtgcagcctt ggaaacctgt
     1741 gatggtaaag tgattcaagg gggaaacatt gaaaacgctt ctctggggtt gtcggtatgt
     1801 gccgaaagag ttgctctttt taaagcagtt tctatgggat ttgctacctt cactcgactg
     1861 gcagtaatat cttccgaaca agattattgt cttccttgtg gtgcctgtcg gcaagttttg
     1921 catgagtttt cgcctcatct tgaaatctta gctgcccgac aggagggaga ttatcaggtt
     1981 tacattttag atgaactcct ccccaatccc tttcagttca atcccgaaaa agggagataa
     2041 gccatggaag atcgggctag ctttcgttc
//
LOCUS       DAOSOL010000260         2058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_54377_length_2058_cov_4.158762, whole genome shotgun sequence.
ACCESSION   DAOSOL010000260 DAOSOL010000000
VERSION     DAOSOL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54377_length_2058_cov_4.158762"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PK171_05975"
     CDS             <1..536
                     /locus_tag="PK171_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT05770.1"
                     /translation="YLQRLKSNGRVYLLGTKELERDFLKVGLVSVQNDPDFIVLGFDK
                     TLTYHKLERACYFIRQGIPFFATHPDINCPTEDGPIPDTGSMIKMIEESTGISPKIFG
                     KPYPEVIEYVSYRTKTTQNEIVMIGDRLYTDIAMGKKSGITTILVLTGETQKDDVKES
                     PWQPDYVFPSLSDIDFN"
     gene            744..>2058
                     /locus_tag="PK171_05980"
     CDS             744..>2058
                     /locus_tag="PK171_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT05771.1"
                     /translation="MKKAYDVIIIGGGVVGCAIAWFLSHFDLKILLLERELDVCCGIS
                     KANTGIIHSRSYLTPGTVKGELHQRALSWFPKIEKELDFHPSVTGALTVAFNQDDLNY
                     LKSLKNIGKYDDAEILSPQESLLLEPNLSDRIVATYYDPHAAVASPFRLTLAFAEGAA
                     LNGVEFHFDSSVEGFDHQGKKIIVHTPNENYEASYVVNAAGLSSAKIAKKSGDQIPVF
                     STFKGEYFLLDKESQGLVKKILYPVPNSLSKGILVSPTPEGNILAGPNFESSCADDTS
                     TTSQGLNEVRAGAQKLVPRFPFNQTIQIFAGIRPTLPERDFLIFVSKKHPGLIHLCGI
                     ESPGLTASPGIAEYVGELLIQQGLSLKEKDQIIFRKAFPVFHDCDWKKREELIAQDPD
                     WGQIVCRCEEVTLAEVKYALRMNPPARTMDGLKRRIRVTAGRCQGS"
BASE COUNT          618 a          378 c          419 g          643 t
ORIGIN      
        1 ggtatttaca aaggttaaag tcaaacggga gagtctatct cttaggtacc aaagaattag
       61 aacgtgactt tcttaaggtt ggtcttgtca gcgttcaaaa cgatccggat tttattgtgc
      121 tcggttttga caaaaccctc acctatcata aactggaaag agcatgctat tttatacgac
      181 aagggattcc attctttgct actcaccctg atattaactg tccgaccgag gatggtccga
      241 taccagacac tggttcgatg atcaaaatga ttgaggaatc aaccgggata tcacctaaaa
      301 tttttgggaa accttatccc gaagttattg aatatgtatc gtatcgaacc aaaaccactc
      361 aaaacgaaat cgttatgatt ggcgatcgtt tgtatactga tatcgccatg ggtaaaaaat
      421 caggaattac tacaatttta gttttgaccg gtgaaaccca aaaagacgat gttaaagaat
      481 ctccatggca accggattat gtctttcctt ctcttagtga tattgatttt aactgatatt
      541 cttattgtat tgactgttgt ttaacaaaga atattatgtt atattaagtc aaagagtatt
      601 aggcttggag aataggagaa agcctttcga gacaattctt tatttgtctc gaaaggcttt
      661 tttgttttat cacacttttt taatgtttct attaatcttc tgtcgcaaaa ttttttattg
      721 aataaatcaa ggagagcatc aatgtgaaaa aggcttatga tgtgatcatt attggaggag
      781 gagttgtggg ttgtgcaatt gcctggtttc tttctcactt tgatttaaaa atactccttc
      841 tcgagagaga gctcgatgtt tgttgtggaa taagcaaggc taatacaggt attattcatt
      901 ctcgtagcta tctcactcca ggaaccgtga aaggggagct ccatcaaaga gctttatcat
      961 ggtttcctaa aattgaaaaa gaactggatt ttcatccttc agttaccgga gcattaactg
     1021 ttgcgtttaa ccaggatgat ctaaactact taaaatcttt gaaaaatatc ggaaaatatg
     1081 atgatgctga gatcttaagc ccacaagaat ctctactact cgagccaaac ctatcagatc
     1141 gaatagttgc tacttattat gatcctcatg ccgcggtcgc gtctcctttt cgccttacct
     1201 tagcctttgc tgaaggggcg gctttaaatg gagttgaatt tcattttgat agttctgtag
     1261 agggttttga ccatcaaggc aaaaagatca tcgtacatac tcctaatgaa aattatgaag
     1321 cttcatatgt tgtcaatgct gctggtctat catcagcaaa aatagctaaa aaatcagggg
     1381 atcagattcc ggttttttca acatttaaag gtgaatattt tttgcttgat aaagaaagcc
     1441 agggcttggt gaaaaaaatt ctctatccag ttcctaactc attaagtaaa ggtatccttg
     1501 tttcaccaac cccagaaggg aatattttag ctgggccaaa ctttgaatcg agttgtgctg
     1561 acgatacctc gaccacatct caagggttga atgaagtcag ggctggtgct caaaagctag
     1621 tgcctcgatt tcctttcaat caaactattc agatttttgc tggcattcgg cctaccttgc
     1681 ccgaaagaga tttccttatc tttgtttcca aaaagcatcc aggtttaatt cacctgtgtg
     1741 ggattgaatc accaggtctt acagcttctc caggaatagc agaatatgtg ggagaattac
     1801 tgattcagca aggattatca ttgaaagaaa aagaccagat aatttttaga aaagcttttc
     1861 ctgtatttca tgattgtgat tggaaaaaac gcgaggaact tattgcccaa gatcccgatt
     1921 ggggtcagat tgtctgccgg tgtgaagaag tcaccctggc ggaagttaaa tatgccttaa
     1981 gaatgaaccc tccggctcga actatggacg gtttaaaacg aagaatcaga gttaccgctg
     2041 gacgctgtca gggaagtt
//
LOCUS       DAOSOL010000261         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_54803_length_2046_cov_3.424912, whole genome shotgun sequence.
ACCESSION   DAOSOL010000261 DAOSOL010000000
VERSION     DAOSOL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54803_length_2046_cov_3.424912"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PK171_05985"
     CDS             complement(<1..301)
                     /locus_tag="PK171_05985"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOT05772.1"
                     /translation="MPRGDGTGPMGMGPMTGRAAGYCAGYDVPGYMNPTFGRFGFGRG
                     WFGRGGGRGWRHWYYATGFPGWARAQYYNPPFGPAWGNVPPPTSPQEEAEVLKEQA"
     gene            complement(325..612)
                     /locus_tag="PK171_05990"
     CDS             complement(325..612)
                     /locus_tag="PK171_05990"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOT05773.1"
                     /translation="MPGLDGTGPLGLGPMTGWGRGYCVTSLGPHRNQFWLGGSPKYFG
                     GHFLGRFGRGRGRGWRHWYYATGFPGWARVQYGYPLVGPMGYYYSPRGNYY"
     gene            complement(673..1137)
                     /locus_tag="PK171_05995"
     CDS             complement(673..1137)
                     /locus_tag="PK171_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOT05774.1"
                     /translation="MRKCWWRGKFEGAGLRLTSPRLAIIDILSKTTTHPSAEDIYLSL
                     RQKFNNDNIGLTTVYRTLEILVNMGIVYKFDFGDGRTRYELSEGPDGYHHHHHLVCTG
                     CGRIVDYTDFIDEEKELLNRTEKGLSKKYHFQIYKHLIQFYGLCENCQLNKS"
     gene            1480..>2046
                     /locus_tag="PK171_06000"
     CDS             1480..>2046
                     /locus_tag="PK171_06000"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOT05775.1"
                     /translation="MDIKNLRKNLQQGKIVVGSEVNEVRSPAIAEVYAAAGLDFIVVD
                     MEHTSFTISEASQIYRMARNCGISPLVRIPAIEYEVICRNLDQGARGIVVPRITSAEE
                     IHQVIEIMKYPPKGKRGLYPGGTAVGYCPTTPADFIQDQNDTTLLIVQIENQQAVQNL
                     DSILSISGIDVILIGPADLSLSIGHPCEF"
BASE COUNT          634 a          449 c          360 g          603 t
ORIGIN      
        1 cagcctgttc ttttaatact tctgcttctt cctgtggact tgttggcggt ggaacatttc
       61 cccaagcagg tccaaatggc ggattataat attgggctct tgcccatccg ggaaatccgg
      121 tagcgtaata ccaatgtctc cacccacgac cgcccccacg gccaaaccaa ccacgtccaa
      181 atccaaatct tccaaacgtt ggattcatgt atcctggtac atcataacca gcacaatatc
      241 cagctgctct ccccgtcatg ggccccatac ccattggtcc ggttccatct cctcttggca
      301 tatcaaaata cctcctttat tcaatcaata ataattgcct ctcggtgaat aataatatcc
      361 cattggtcct actaaaggat aaccatactg gactcttgcc catccgggaa atccggtagc
      421 gtaataccaa tgtcgccacc cacgacctct cccccgacca aaccgaccca gaaaatgtcc
      481 accgaaatac ttaggtgaac ctcctaacca aaactggtta cgatgtgggc caagagaggt
      541 tacacaataa cctcttcccc atccagtcat cggtcctaaa cccaaaggtc cggtgccatc
      601 aagtccgggc atttcataca cctcgctttt attgctattg aaaaccattt tcatttataa
      661 aagaaaaaaa atctatgatt tattcagctg acagttttca cataagccat aaaattgaat
      721 aaggtgcttg taaatctgga aatggtattt cttagaaagg cctttttcag ttcgattcag
      781 caactctttt tcttcatcaa taaagtcggt ataatctact attcgaccac atccggtaca
      841 aaccaaatga tggtgatgat gataaccatc cggtccctcc gaaagttcgt accgtgtacg
      901 accgtcacca aaatcaaatt tataaacaat tcccatattc actaaaattt ctaaagtccg
      961 gtaaactgta gtgagcccaa tattatcatt attaaatttt tgtcgaaggc taagataaat
     1021 atcctccgca ctggggtggg tagttgtttt actgagaata tcgataattg ccaaacgtgg
     1081 tgaggtcaat cgcaaacctg ctccttcaaa tttccctcgc caccagcatt ttcgcatttt
     1141 ctatgagctc cttttatgca tcttattgaa aatggttttc attattattt atattatctg
     1201 accttttagt tcttttgtca agaaaaaata actggttttg aaagttcttt ttctaaatat
     1261 taaaaattta atgatcattt gtaaattcat atgaggatga taaagatcat ttgggaaaga
     1321 aaaaatggga gaaggttggg aagagggtga tatgtattaa ccggataaat aaattatatc
     1381 tatatcataa taaaaaatag gtattatcgt ataatatgaa aacgaaattt ataaaaatct
     1441 cggtaaataa tgaataaccg gttaaggatg gatcaaaaaa tggacattaa aaatcttaga
     1501 aaaaatctac aacaaggtaa aatcgttgtt ggaagtgaag taaacgaagt tcgatctcca
     1561 gccattgccg aagtatatgc agccgctggg ttggatttta ttgtagttga tatggaacat
     1621 acttcattta caatttccga ggcttctcaa atatatcgaa tggcaagaaa ttgtggcatt
     1681 tcaccgttgg ttcgtattcc agccattgaa tatgaagtaa tctgccgaaa tcttgaccaa
     1741 ggcgctcgag gaattgtcgt tcctcgaatt acttctgccg aagaaatcca ccaagtcatt
     1801 gagattatga aatatcctcc taaagggaaa agaggccttt atccgggagg aactgcagta
     1861 ggttattgcc caactactcc agctgacttt attcaagatc aaaatgatac gactctgctc
     1921 attgttcaaa ttgaaaacca gcaagcagtt cagaatttag acagcattct ttccatctcg
     1981 ggaatcgatg tgattctaat cggcccggct gacctttctc tttcaatagg tcatccctgt
     2041 gaattc
//
LOCUS       DAOSOL010000262         2040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_55034_length_2040_cov_3.215617, whole genome shotgun sequence.
ACCESSION   DAOSOL010000262 DAOSOL010000000
VERSION     DAOSOL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55034_length_2040_cov_3.215617"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..461)
                     /locus_tag="PK171_06005"
     CDS             complement(132..461)
                     /locus_tag="PK171_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05776.1"
                     /translation="MKNIAVFFLIFMVSMSFLYLLLQAEIVEKGFVLSLKKERIESLV
                     ANKDRIEIEISHLSSMDRIKEIATNKLGMVLPERTIFLAAADQKVQLKGSEAAVAEYR
                     NETGLKR"
     gene            complement(458..1357)
                     /gene="rsmH"
                     /locus_tag="PK171_06010"
     CDS             complement(458..1357)
                     /gene="rsmH"
                     /locus_tag="PK171_06010"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT05777.1"
                     /translation="MFHYHQPVMVVEVIQYLVTDPNGVYVDATVGGGGHAKAILEKVA
                     QQGVLVGIDRDREAIDAARQVLAVFGSRVKLVQGSFSTIAEILHQEKIETIHGVLFDL
                     GVSSRQLDAPERGFSFSQDGPLDMRMDQSQKMDACQVVNTYSEKELADIFFYYGEERK
                     SRKIAHSIVAARQRKPIATTGELEKIIWKCNPGRRGGIHPATRVFMALRIYINHELDE
                     LPKALESVVQFMNEGARLVVLSYHSLEDRIVKNFFRNSQQLEVINRKPMIPSRQEIQS
                     NPRARSARMRVAQKMMREEGTPA"
     gene            complement(1359..1790)
                     /gene="mraZ"
                     /locus_tag="PK171_06015"
     CDS             complement(1359..1790)
                     /gene="mraZ"
                     /locus_tag="PK171_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT05778.1"
                     /translation="MLLGQYRHSVDNKGRLIIPNEFRKDLSNTLYVTKGIENCVFVFS
                     ETAWQVLAGKVASLPMTKKAGREFARIFLANASEETIDAQGRITIPRHLREYAEIDKK
                     VVTVGVGERMEIWSESVWDQYASEVEGKYEDITEEIMDTGI"
BASE COUNT          597 a          487 c          315 g          641 t
ORIGIN      
        1 catgttggct ttttcccgat actcgtttcc ttcaacaact tgcaggacga acattcgaaa
       61 aacaatatat cctaaaatca aaaaaaccat catcaccaga aaatttgccc gatggacaaa
      121 tcttctggag ctcatctttt caaccccgtt tcatttctat attctgcaac cgctgcctca
      181 ctccccttaa gctgaacttt ttgatcagca gcagcgagaa atatcgtcct ctccggtaaa
      241 accataccta atttattagt ggcaatttct ttaattcggt ccattgacga aagatgagaa
      301 atttcaattt caatgcgatc tttattagca accaggcttt ctattcgttc tttctttaaa
      361 cttaaaacaa atcctttttc gacaatttca gcttgcagta acaaatacaa aaaagacata
      421 ctcaccatga atattaaaaa aaatacggca atatttttca agctggagtc ccctcctctc
      481 tcatcatctt ttgagcgact ctcattctgg cactccgagc acgaggattc gactgaattt
      541 cttgccgaga aggtatcata ggcttgcggt tgatgacctc aagttgttga ctatttcgaa
      601 agaaattttt aacaattcga tcttcaagag aatgatagct gagaaccacc agacgtgctc
      661 cttcattcat aaattggacc acactctcta aggccttcgg taattcatct aattcatgat
      721 taatataaat ccgtaaagcc ataaaaaccc gagttgctgg gtgaataccc cctcttcgac
      781 ccggattaca tttccaaata attttttcca actctccagt tgtagcaatt ggttttctct
      841 ggcgagcagc tacaatcgaa tgagcaattt tacgtgattt tcgctcttca ccgtaataaa
      901 agaaaatatc agccaattct ttttcagaat aggtgttaac aacctgacag gcgtccattt
      961 tctggctttg atccattctc atatccaatg gtccatcctg actaaagcta aaacctcttt
     1021 ctggagcatc taactgtcga gaagaaacgc caagatcaaa taaaactcca tgaatagttt
     1081 caattttttc ttgatgaaga atttctgcta tcgtcgaaaa agatccttga accagtttta
     1141 cccggcttcc aaaaacggcc aacacttgcc ttgctgcatc aatagcctcc cggtcacgat
     1201 cgattccaac taaaactccc tgttgagcaa ctttttccaa tattgctttg gcgtgacctc
     1261 caccaccaac cgtagcatca acataaacgc cattaggatc ggtaaccaaa tactgaataa
     1321 cctcaacaac cataaccggc tgatgatagt gaaacacctc aaatccccgt atccataatt
     1381 tcttctgtta tatcttcata ttttccttcg acttctgagg catattgatc ccaaaccgat
     1441 tccgaccaaa tttccattct ttctccaact ccaaccgtga ccactttttt atctatttct
     1501 gcatactctc ttaaatgccg gggaatagta atacgtcctt gagcatctat ggtttcttca
     1561 ctggcattgg ctaagaaaat ccgggcaaat tctctccctg ctttcttggt catagggaga
     1621 gatgcaactt tccctgctaa aacctgccag gctgtttcgg aaaaaacaaa tacacaattt
     1681 tcaatacctt tggtaacata gagggtgttg gataaatctt ttctgaattc attgggaatg
     1741 atgagtcttc ccttattgtc aactgaatgt cgatattgtc ctaatagcaa tcggacccct
     1801 cctgataacc acttttcccc acttttcccc acaatttacc acaaatatac cactcttctc
     1861 tctttttgta aatgcaatac gctattttta caaaaatatt taaattttaa gaaataaatg
     1921 atgatttctg gctcattttg tggtggtcat tacctgagaa aaatgccgag ggggagaggg
     1981 aagatacagc gaggtgtgcc ttttcttttt ttaatcattt tcacttgttc aggtattttc
//
LOCUS       DAOSOL010000263         2027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_55517_length_2027_cov_2.920385, whole genome shotgun sequence.
ACCESSION   DAOSOL010000263 DAOSOL010000000
VERSION     DAOSOL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55517_length_2027_cov_2.920385"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     misc_binding    complement(55..280)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            422..946
                     /locus_tag="PK171_06020"
     CDS             422..946
                     /locus_tag="PK171_06020"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT05779.1"
                     /translation="MKKSNLFFYSFLTTLLGTAVIGWQNDLLAAFVLPQPTPEEAFQY
                     LQSYHLPQHNSFVLETKLPWPDFSDKVNTYFSGKKVEFTEPVIIEQFPDYTRQILDIT
                     KNIPWGEVKTYQQVAQLSSRKRAYRAVGQALHRNPIPLIIPCHRVVAKSSLGGFGYGL
                     DWKLRLLTLENIQL"
     gene            complement(1096..1662)
                     /locus_tag="PK171_06025"
     CDS             complement(1096..1662)
                     /locus_tag="PK171_06025"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT05780.1"
                     /translation="MKCRNLVYTGAFSALSIVLSYLIHFPLIPSAPFLLYDPGDVPIL
                     IMGLKFSPGIGVVMTAIVSVLFAVLTGQGGPWGVLMHFIATGVYVLTASLIYRRQRNL
                     SSAVKGLIIAPLVMTGVMVIANLLVTPLYLGVTREMVIGMLLPAIIPFNLLKGAINGI
                     ITFLLYKKVSNFLEAGDQCVSKPNAVRQ"
     regulatory      complement(1748..1889)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT          631 a          458 c          362 g          576 t
ORIGIN      
        1 aacaagaacc tttctcatat tgtacctcct tttttaaaaa aaaatccctc ccgacacagg
       61 gacgggaggg attaatgacc cgcggtacca ccctcattga gcaagaggct ccagctttaa
      121 acttttaacg cagcacgcga tgaagctgct gaccctttct tcaaccagct tcactcctcc
      181 aggctccatt cgcctcatcg caaacaccag gtttccagcc cctctcccgg ctctctatag
      241 tttgtaggat gaaactactt gttcccttct tcggattttt acgatattat taacagtata
      301 tcaaaaatta tacggtaatt ttcaatcttg taaagaggta atagtcactt tttttgcaaa
      361 aaaccattaa aatactcatt taaagaccaa tttgataaga aaatggaaag ggtgggtctt
      421 ggtgaaaaaa tcaaatctct ttttttattc atttctaacc acactattag gtacggcagt
      481 tattggctgg caaaatgacc tgctggctgc atttgttcta cctcaaccaa cccctgaaga
      541 agctttccaa tatctccagt cctaccatct cccccagcac aattcattcg tacttgaaac
      601 taaattgccc tggccagatt tttctgataa ggtcaatact tatttttccg ggaaaaaagt
      661 tgaatttact gaaccagtta ttattgagca atttcccgat tacacccgcc agatattgga
      721 tatcaccaaa aatatacctt ggggagaagt aaaaacctac caacaggttg cacaattatc
      781 ctcacgaaaa agagcttatc gggcagtagg ccaagcttta catcgtaatc cgattccttt
      841 gattatcccc tgtcaccggg tcgtcgccaa aagttcttta ggaggattcg gttacgggtt
      901 agactggaaa ttacgtttac tgaccctcga aaatattcag ctataaaaac aaaattcttt
      961 cctgaaatta tttttttaaa atttacaaaa tgagattgcc acgtcgttca accaaaaaac
     1021 ggttggactc ctcgcaatga cggatttaga taggtatttt catcctcatc tggtgttgtt
     1081 aggcagtatg agggtttatt gtctaacagc attgggtttt gaaacacact gatcgccagc
     1141 ctcaagaaag ttgctgactt ttttatagag caaaaatgta ataattccat taatagcacc
     1201 tttgagaaga ttaaagggta taatggcagg aagcaacatg ccgataacca tttctcgagt
     1261 cactcctaag tacaaaggcg taaccaaaag attagctatc accataaccc ccgtcatcac
     1321 caaaggagca ataatcaaac cctttactgc tgaagagagg tttctttgtc ttcggtaaat
     1381 caaactagca gtcaatacat atacaccagt agctataaaa tgcataagca ctccccaagg
     1441 acctccctgc ccggttaaaa ccgcaaataa aactgataca atggcagtca tgaccacgcc
     1501 aattccagga ctgaatttaa ggcccataat caatatgggt acatcaccag gatcataaag
     1561 caaaaagggt gccgaaggaa ttagggggaa gtggattaaa tagctcaaaa caatggatag
     1621 agcagaaaat gctccagtat aaaccaaatt tcgacacttc atatcaagaa gcctcctcga
     1681 atggtctttc ttcagagata aaaaaaccct gaaagcattt cgccttcagg gtttatgggc
     1741 ttattatgga cttcttctcc catccggact ttaccgtcgg ctccggattt tcaccggatc
     1801 aacccatatc gggctcgcgg gcttaggatg aaccatcacc gccggttggg aattgggaat
     1861 ttctccctta ccctaccctg aagaagtatt ttctcaatga tttattctaa cttgatattg
     1921 ttaattgtca agtgattcag atcttatttc gataatgaga agccaagaaa agaggaagct
     1981 tttcatactc ttcatataat ttttgataat gcggccaatt ttccgaa
//
LOCUS       DAOSOL010000264         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_55799_length_2021_cov_2.472024, whole genome shotgun sequence.
ACCESSION   DAOSOL010000264 DAOSOL010000000
VERSION     DAOSOL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55799_length_2021_cov_2.472024"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PK171_06030"
     CDS             <1..456
                     /locus_tag="PK171_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOT05781.1"
                     /translation="QIEESLSTFQGLSHRIQKVAEINGVVFYDDSKSTTPASTCAAIN
                     SLNGPIVLLLGGRSKIKDFSEFTQYLSPAKIKMILLFGEDRELIQKFIPESIPTRLFS
                     NLDEIFSNIRSLVVPGDCVLLSPACTSWDQYANYQERGEHFFRLVYGNS"
     gene            464..1621
                     /gene="ftsW"
                     /locus_tag="PK171_06035"
     CDS             464..1621
                     /gene="ftsW"
                     /locus_tag="PK171_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOT05782.1"
                     /translation="MNRWSLENSNENHLKRWWWEIDPYLFWTMILLLCIGLIMVFSAS
                     MTTSIMSYSDPYYFFKKQVVGVALSFFFFIIASFIRLDFVRKMSSKLLLLSVVLLTLV
                     FFPYIGRMVGGSYRWIDFGIMRFQPSELAKLSLIIYMADSLVTHRHRAQDFWYGVVPF
                     LLIVGVMGVLVIMEPDLGTALFLAGITFGMLFLGGRKIIHILYPIFAGIPILLFLVFA
                     SGNQYWKARLEAFVDPWKEPLGKGFQIIQSLIAIGSGGLFGRGLGESRQKFFYLPDRH
                     TDFIFAIIGEELGFVGTIGVVILFCFLFWRGWKIAVRSDDDFQAMLAMGIILSIMMQV
                     MVNMSAVLKILPVSGVPLPLVSYGNSSILITMIEIGLLFNIARQSKVNHFE"
     gene            1614..>2021
                     /locus_tag="PK171_06040"
     CDS             1614..>2021
                     /locus_tag="PK171_06040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT05783.1"
                     /translation="MNEIFIAAGGTGGHIFPALSIAEHLSRDYPVRFIGTKRGMEKIL
                     VPQAGFSLSFIRARGWNRTSALDFLRAFGDNIIGLMQSGYLFIKFRPRAVLVFGSYVS
                     LLIAFWAKLFRVPIFVQEQNIRPGFANRIISRWS"
BASE COUNT          499 a          355 c          446 g          721 t
ORIGIN      
        1 caaattgaag aatctctttc aacttttcag ggattgagtc acagaattca gaaggttgct
       61 gaaataaatg gtgttgtatt ttatgatgat tccaaatcga cgacaccagc atcaacctgt
      121 gcagcaatca attccttgaa tggtcctatt gttttgctct taggggggag aagtaaaatc
      181 aaagattttt ctgaattcac ccaatatctt tctccggcta aaataaaaat gatccttcta
      241 tttggagaag accgagagct gatacagaaa tttataccgg aatctatacc aacccgttta
      301 ttttctaatt tagacgaaat attttcaaac atccgctcct tagtcgtacc cggtgattgc
      361 gttctcttgt ctccggcatg taccagctgg gatcaatatg ccaactacca ggaaaggggt
      421 gaacactttt tccgtttagt ctatgggaac tcatgaagaa ttattgaacc ggtggtcatt
      481 agaaaattcc aatgaaaatc atttaaaacg ctggtggtgg gaaatcgatc catatctttt
      541 ttggacgatg attctcttac tctgcattgg tttaatcatg gttttcagtg ccagcatgac
      601 cacgtcgatc atgtcctata gcgatcctta ttattttttt aaaaagcagg tggttggagt
      661 tgctctgtct ttcttttttt tcataatcgc ctcttttatc cggcttgatt ttgttagaaa
      721 aatgagctcg aaattacttc ttctatcagt cgtgctactc actctggttt tcttccccta
      781 tatcggcaga atggtgggtg gatcctatcg atggatagat tttggtatca tgcgttttca
      841 accatcagaa ttagctaaat tgagtttaat tatttatatg gctgatagtt tggtgaccca
      901 tcgtcatcga gctcaagatt tttggtatgg agtggtgccg ttcctcttga ttgttggtgt
      961 catgggtgtt ttggtcatta tggaacccga tcttggcacg gctctttttt tagctggtat
     1021 aacatttggt atgttgtttt tgggagggcg aaaaattatt cacatcctct atccaatttt
     1081 tgctggaatt cccatcttgc tctttttggt ttttgccagt gggaaccaat attggaaagc
     1141 acggcttgag gcttttgttg atccttggaa agaaccatta gggaagggat ttcaaattat
     1201 ccaatctttg attgccatcg gttccggagg cttgtttggt cgcggtttag gagagagcag
     1261 acaaaaattt ttctatcttc ccgatcgaca tactgatttt atttttgcca ttattggtga
     1321 agaattaggt tttgtaggga cgattggagt ggttatttta ttctgctttc ttttttggag
     1381 gggatggaaa attgcggtac gatcggatga tgattttcaa gccatgttgg caatgggaat
     1441 aatcctgagt attatgatgc aggtcatggt caatatgagt gctgtgctta aaattcttcc
     1501 agtatctggt gttcctttac ctttggtgag ttatggtaac tcatcgatcc tgattacgat
     1561 gattgaaatc ggtttgttgt tcaatattgc tcgacaatca aaggtgaatc attttgaatg
     1621 aaatatttat tgcggctggt ggaactggtg gtcatatttt tccagcactc agtattgctg
     1681 aacatctttc tcgtgattat ccggtacgat ttattggaac aaaaagaggg atggaaaaaa
     1741 ttttagttcc tcaagccggt ttttcgctgt catttattcg ggcgcgcgga tggaatcgaa
     1801 cctcggctct ggattttctc cgtgcttttg gtgataatat aatagggtta atgcaaagtg
     1861 gatatctttt tatcaagttt cgacctcgag cggttttggt ttttggaagt tatgtttctt
     1921 tactcattgc attttgggcg aaattgttcc gggttccaat ttttgtgcaa gaacaaaata
     1981 tccgacccgg ttttgccaac cggattatat cccgttggtc a
//
LOCUS       DAOSOL010000265         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_56105_length_2012_cov_4.252938, whole genome shotgun sequence.
ACCESSION   DAOSOL010000265 DAOSOL010000000
VERSION     DAOSOL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56105_length_2012_cov_4.252938"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..678
                     /locus_tag="PK171_06045"
     CDS             91..678
                     /locus_tag="PK171_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT05784.1"
                     /translation="MIYYISKYLGLFLLYLFCRFRVSGRKNFPKSTPCLIVSNHASYL
                     DPIVIGCACPRRVYFVAKEELFQNPVAKFFLNQLGAFPLRRGEADRSAVKKIFALIKE
                     NKVVCLFPEGTRNQGEVGDFRSGAIKLLMKAKADVVVAGVTGTFESYPRGARFPKPHP
                     IQVHFSEPLPQNGLNQSEWEERLKRKMEEFIHDDR"
     gene            665..>2012
                     /locus_tag="PK171_06050"
     CDS             665..>2012
                     /locus_tag="PK171_06050"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HOT05785.1"
                     /translation="MMIGKKLDWCLTGLISFFVVIVIGSSVQAQIEKIELSIEEVTDQ
                     KTPYLNYPVSVIVQEESGLVLISDWANDRLVITDQNGKTQKIIPGLEGPVGIVYDSPT
                     NRLYLTEQKANRIRILDGSTFAPISELKIQGITLNEPRGLWMDNGRNIYLVDTMNSRI
                     VIFDQEGNLIRTIGKEGMGDDEFYYPRGISVDSQGRIWMTDTLHHTVKVFDSVGNYLF
                     RIGKGGSGPDDLDRPRYVIVKDETVIISDYRNNRLKIHNTQGELIGIVDRIGGDHLLN
                     PEGLWIDGNGYLWVADSGNNRVVKLDMTFLMNREAYLASLLSEDKIDEFLREAASLSP
                     EKRQKPEISALFYAAYQKKDDLENMIAEAENIWMKDEENRSTWSEELGILYYRKATLS
                     RAAQPAATVKALYRKSIQYGYKKAYIPYIWTSFLMLGGSNLFLIILIILLLVLLFVL"
BASE COUNT          576 a          369 c          457 g          610 t
ORIGIN      
        1 tttacatcat ctggtttaca gccttcaaga atagaattac aatacccata ggtttggtgg
       61 tttatttttt tttaaaaagc cgaggatttt atgatttatt acatcagcaa atacttagga
      121 ttgtttcttc tctatctctt ttgccgcttt cgggtaagtg gccgtaaaaa tttcccaaaa
      181 tcaactccct gcttaatcgt ttctaatcac gccagttatt tggatccgat agttatcggc
      241 tgtgcttgtc ccaggcgagt ctatttcgtc gctaaagaag aattgtttca aaatccggtt
      301 gctaaatttt tcctcaatca gttaggagcc tttcctttga gaaggggtga agccgaccgc
      361 agcgcagtta agaaaatatt tgcactcata aaagagaata aagtggtgtg tctatttccg
      421 gaggggacca gaaaccaagg tgaagttggt gattttcgct ccggtgcgat aaaattattg
      481 atgaaagcca aagctgatgt ggtagtggct ggggtgaccg gaacttttga aagctatccc
      541 cgcggagcaa ggtttcccaa acctcatccg atccaggttc atttctctga gcctctaccc
      601 caaaacggtc tcaatcagtc tgaatgggag gaaagattaa aaagaaagat ggaggagttc
      661 attcatgatg atcggtaaaa agctcgattg gtgcctgaca ggtctcatct ctttttttgt
      721 ggtgattgtt attggttctt cggttcaagc ccaaatcgaa aaaatagagt tgagcattga
      781 agaagttact gaccaaaaaa ctccctattt gaattatcca gtaagcgtta ttgtacagga
      841 agaaagcggc ttggtattga ttagcgactg ggctaacgac cgcctggtta ttaccgatca
      901 aaacggaaaa acccaaaaaa ttatccccgg cttggaaggt ccagtaggga tagtttatga
      961 ttcccccacc aaccgcctct atcttactga gcaaaaagcc aaccgaattc gaattttgga
     1021 tggttctact tttgcgccga tcagcgagct taaaattcag ggaattactc tgaatgaacc
     1081 tcggggtctc tggatggata atggccgtaa tatctatctg gttgatacta tgaattctcg
     1141 gattgtgatt tttgatcaag aagggaatct gatcaggact attgggaaag agggaatggg
     1201 agatgatgaa ttctattatc cccggggtat atctgttgat tcccaaggaa gaatatggat
     1261 gaccgatact cttcatcaca cagtgaaggt gtttgattca gtgggaaatt atctttttcg
     1321 tattggaaaa ggtggcagtg gtcccgatga tttagatcgg cctcgatatg taatcgtaaa
     1381 agatgaaacg gtgattattt ctgattatcg aaataaccgt ttgaagattc ataatactca
     1441 aggtgagctg attgggatag tcgatagaat tggtggtgat cacctcctca atcctgaagg
     1501 gttatggatc gatgggaatg gttatctttg ggtcgccgat tccggtaata atcgggtggt
     1561 caaactggat atgacctttt taatgaaccg ggaagcctac ctggcatctt tattgtccga
     1621 agacaagatc gatgaatttt taagagaagc tgcctctctt tctccagaaa aacggcaaaa
     1681 acctgaaatc agtgctcttt tttatgctgc ttaccagaaa aaagatgatt tagagaatat
     1741 gattgctgag gctgaaaata tttggatgaa agatgaggag aaccgctcaa cctggtcaga
     1801 agaattgggg atactttact accgaaaagc gaccctatct cgggcagccc aacctgctgc
     1861 aacggtaaaa gcattatatc gtaagtcaat tcaatatggt tataaaaaag cttacatccc
     1921 ctatatttgg acatcatttt tgatgttggg tggatccaat ctttttctga ttatccttat
     1981 aattttgctt ttagtattgt tgtttgtgct at
//
LOCUS       DAOSOL010000266         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_56113_length_2012_cov_3.182933, whole genome shotgun sequence.
ACCESSION   DAOSOL010000266 DAOSOL010000000
VERSION     DAOSOL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56113_length_2012_cov_3.182933"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="PK171_06055"
     CDS             <1..1026
                     /locus_tag="PK171_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05786.1"
                     /translation="FDPLRIKRQWKDQVMEISPVEVHEVDAHNIVPVWVTSSKQEYGA
                     YTIRPKISRLLPEFLDEFPPIKMKVSHQSWQGNLVHWDELLLQCPGDASVPPVIHFSS
                     GSKNAVKHLQQFLEEKLEFYNSQRNDPNADAQSNLSPFLHFGQISAQRIALEVQKASV
                     SRDKKAAFLEEVIIRRELSDNYCWYNINYDKFIGFPSWAQITLNQHRKDQREYQYSLE
                     KFEKAQTHDQLWNAAQKEMVFTGKMHGYLRMYWAKKILEWSETPEVALENAIYLNDRY
                     ELDGRDPNGYTGIAWSIGGVHDRAWPERPVFGKIRYMSYRGSRSKFNVDQYISQIKDL
                     EGTERKD"
     gene            1031..1993
                     /locus_tag="PK171_06060"
     CDS             1031..1993
                     /locus_tag="PK171_06060"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOT05787.1"
                     /translation="MQFSKPTVVVSKCLGFDSCRYNGQMILVDFIEKMKPYVHFVPVC
                     PEVAIGLGVPRDPIRIVSINGDLRLVQPSTSHDYSKKMEQFALEFLHRLPEVDGFVLK
                     GRSPSCGIKEVKIYQTMDKGPSIGTTQGFFGKAVLNTFPFLPIEDEGRLSNLKIRDHF
                     YTSLFVLADFHQLSIQPSRHQLVQFHTELKLLLMAYNQSEMRMMGRIVAQVNKKPIKE
                     VFEDYRSHLLKALFRPPKTPSMINVFMHALGYFSTRLQTNEKAFFLDSLEKYRAGRST
                     FATNLQLLRSWVIRFDEPYLKNQRFFEPYPEGLMELVDSGKGRE"
BASE COUNT          623 a          371 c          421 g          597 t
ORIGIN      
        1 tttgatcctc tcagaattaa gagacaatgg aaagatcaag taatggaaat atccccagtt
       61 gaagtccatg aagtcgatgc gcacaatatt gttccggtgt gggttacttc ttccaaacaa
      121 gagtatggag cttatacgat tcgacccaaa atttcacgat tattacctga attcctcgat
      181 gaatttccgc ctataaaaat gaaggtttcc catcaatctt ggcaagggaa tttagtgcat
      241 tgggacgaat tacttcttca gtgtccagga gacgcatccg ttccgccggt cattcatttt
      301 agctccggat caaaaaatgc ggtgaaacac ctccaacaat ttcttgaaga aaagcttgaa
      361 ttctataact ctcagcgtaa tgatccaaac gctgatgctc aatcgaatct atcacccttt
      421 ctccactttg gtcaaatatc agcgcaaaga attgccttgg aagtccaaaa agcctcggtt
      481 tcaagggata aaaaggccgc ctttttagaa gaagtcatta taagacggga actatcggat
      541 aattattgct ggtacaatat caactatgat aagtttattg gtttcccatc ctgggcgcaa
      601 ataactctca atcagcaccg taaagatcag agagagtatc aatattcatt agaaaaattt
      661 gaaaaggctc aaactcatga tcagctatgg aatgcagctc aaaaagaaat ggttttcact
      721 ggtaaaatgc atggatattt gagaatgtat tgggcgaaaa aaattttaga atggtcggaa
      781 actcctgaag ttgccttaga gaacgccatc tatttaaatg atcgatatga attggatgga
      841 agagatccaa atggatatac tggcattgct tggagtatcg gaggtgtgca tgaccgcgcc
      901 tggcctgaaa gaccagtgtt tggaaagatc cgatacatga gctatcgggg aagtcgttcg
      961 aaatttaacg ttgatcaata catctctcaa attaaagacc tggaagggac agaaaggaag
     1021 gattgagttg atgcagtttt ccaaaccaac cgtggtggtt agtaagtgtt taggctttga
     1081 ttcttgtcgg tataatggtc aaatgatttt agttgatttt atcgaaaaaa tgaaacccta
     1141 tgttcatttt gttccggttt gtcctgaagt ggctattggt ttaggagttc cacgtgatcc
     1201 aatacgcata gtgtctataa atggagattt gaggctggta caaccatcaa ccagccatga
     1261 ttattctaaa aaaatggaac aatttgccct ggaatttttg catcgtttac ctgaggtaga
     1321 cgggtttgtt ctgaaaggac gatccccttc ttgtggaata aaagaggtca agatctatca
     1381 aactatggat aaaggaccat caatagggac aacccaagga ttctttggca aggcagtatt
     1441 aaacacattc cctttcttac caattgagga tgaagggcgt ttatctaatt taaaaattcg
     1501 agatcatttt tatacatcct tatttgtttt ggcagatttt caccaactga gcattcaacc
     1561 atcacgacat caactggttc aatttcatac cgaactaaaa ttacttctta tggcttataa
     1621 tcaaagcgaa atgaggatga tggggagaat tgttgctcaa gtgaataaaa agccgataaa
     1681 agaagttttt gaagactatc gatctcatct tttaaaagct cttttcagac cacctaaaac
     1741 tccctcgatg atcaatgttt ttatgcatgc cctgggttat ttttctaccc ggcttcaaac
     1801 taatgaaaaa gctttttttc tcgattcttt agagaaatat cgggcgggtc gaagcacctt
     1861 tgcaaccaac cttcagctcc ttcgttcctg ggttattcgt tttgatgaac cttatttaaa
     1921 aaatcagcgg ttttttgaac cttatcccga aggacttatg gaattggtgg attctgggaa
     1981 aggtcgagaa tagaggagtg gggacattat gg
//
LOCUS       DAOSOL010000267         2011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_56149_length_2011_cov_3.364008, whole genome shotgun sequence.
ACCESSION   DAOSOL010000267 DAOSOL010000000
VERSION     DAOSOL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56149_length_2011_cov_3.364008"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PK171_06065"
     CDS             <1..273
                     /locus_tag="PK171_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HOT05788.1"
                     /translation="AKALRILRSRLLEIEQQKQKAEIDRDRKSQIGSGERSERIRTYN
                     YPQNRVTDHRINLTLYKLDLILEGDLNEVIESLAAEERMRMLEKVG"
     gene            277..1143
                     /gene="prmC"
                     /locus_tag="PK171_06070"
     CDS             277..1143
                     /gene="prmC"
                     /locus_tag="PK171_06070"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT05789.1"
                     /translation="MSKVRDVWSNLVLTLRKHNLPGASREARFLLSGVLSASPSHIYL
                     YWDQDLSVEKIEKLNIMIKERLDGVPLQYILKEWEFFSLQFYLEKGVFIPRLDTESWL
                     EEAIITIRSRYENQPVKLCDLCCGSGVIGLTVAYWLTKVQVVAIDRSKLACDLTQHNA
                     QKLGLEERISVWQSDLFNEIQTTDELQFDFILSNPPYVKEEEWNTLSPEIRLHEPKDA
                     LVSGSEGLDLIYRILNESSPYLKSQGYLYIEHDPSQVEAIADLSLKTHFKKDHVIFDY
                     QKRPRATVFRRN"
     gene            1416..1853
                     /gene="rpiB"
                     /locus_tag="PK171_06075"
     CDS             1416..1853
                     /gene="rpiB"
                     /locus_tag="PK171_06075"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOT05790.1"
                     /translation="MKIALGCDHGGFELKEDLKNFLIEQGYEVIDYGTNSPEMIDYPD
                     ITFPLAKDVAKGKCERGIICCGTGVGVSIAANKVHGIRAANCHDTFSARASREHNNAN
                     ILTLGGRVIGKGLAQEIVQVWLKSEFIEGRHLRRINKISDYEK"
     gene            1853..>2011
                     /locus_tag="PK171_06080"
     CDS             1853..>2011
                     /locus_tag="PK171_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOT05791.1"
                     /translation="MNTRPDWDEYFMSIAELVSTRSTCLRRKVGAVLVLDRRILATGY
                     NGAPSGLAH"
BASE COUNT          667 a          331 c          424 g          589 t
ORIGIN      
        1 gccaaagctc tgcgaattct tcgttcaagg cttttagaga ttgaacaaca aaaacaaaaa
       61 gctgaaatcg atcgtgatcg aaaatcccaa attggcagtg gagaaagaag tgagagaatt
      121 cggacttata attatcctca aaatagagta accgatcatc ggattaattt aactctttat
      181 aagctggatt tgattttaga aggcgatctc aatgaagtca tcgaatccct ggcagctgag
      241 gaaagaatga gaatgttgga gaaagtggga taactcatgt caaaagtgag agatgtttgg
      301 agcaatctgg ttctcacttt acgtaaacat aacttacccg gagcctcccg agaagctcgt
      361 tttttgttgt ctggagttct ttctgcttca ccttcacata tttatcttta ttgggatcaa
      421 gatttgtcag ttgaaaagat tgaaaagctc aatatcatga taaaagaacg acttgacgga
      481 gttcctcttc aatacatact caaagaatgg gagttttttt ctctccaatt ttatttagag
      541 aaaggagttt ttataccaag gttggatact gaatcttggt tggaggaagc aattattacc
      601 atcagatctc gctatgaaaa ccaaccagtc aagttgtgtg atctttgttg tggttcaggt
      661 gtaattggtt taaccgttgc atattggctg accaaagtac aggtagttgc cattgatcga
      721 tcaaaattag cctgtgattt gactcaacat aatgcccaaa aattgggctt agaagaaaga
      781 atatcagtat ggcaatcaga tttgtttaat gaaatccaaa ctactgatga actacaattt
      841 gatttcattt tgtcaaatcc accttatgtt aaagaagaag aatggaatac cctgtctcct
      901 gagatacgac ttcatgaacc aaaagatgcg ttggtttcgg gttcagaagg cttggacctc
      961 atctatcgaa tcttgaatga atcatcccct tacttgaaaa gccagggtta cctttatatc
     1021 gaacatgatc cttctcaagt tgaagccatt gcagatcttt ctttaaaaac ccattttaaa
     1081 aaagatcatg tcatttttga ttatcaaaaa agacctcgag ctacagtttt tagacgaaat
     1141 tgagtagggg aaaaaaagaa agacactttt cccaccacat tcctccaaga aagagaaatt
     1201 attaaaaaga tacttccaca ccttaatctt tttctactag aagagagatg aaaaaagaaa
     1261 aaaagaatta atacttcctt ctcacttaat gggagaaggt ggagatgagt ttgaaaaaaa
     1321 gagatttcat gatattttta aacttataaa cactgttggg gtagacaaaa caagggtttt
     1381 aagagtatcc ttttaaaagg aggttctaat agacgatgaa aatagctttg ggttgcgatc
     1441 atggaggctt tgaactcaag gaagatttga aaaatttttt aatagaacaa ggatatgaag
     1501 tgattgatta tgggactaat agccctgaaa tgattgatta tcctgatatc acatttccct
     1561 tggctaaaga tgtagccaag ggaaagtgcg aaagaggaat tatctgttgc ggaaccggcg
     1621 ttggtgtctc aatagctgcc aataaagttc atggaattcg agcagccaac tgtcatgata
     1681 ctttttcagc aagagcgtca agagaacata ataatgcaaa tattctcact ctcggaggac
     1741 gagtgattgg aaagggtcta gcccaggaaa ttgttcaagt ttggttaaaa tcagaattta
     1801 tcgagggtcg acacctgaga cgtatcaata aaatctcgga ttatgaaaag taatgaacac
     1861 acgaccggat tgggatgaat attttatgtc aattgccgag ttggtcagca ctcggtcgac
     1921 ctgtttaaga agaaaagttg gagcggtgct ggttcttgat cgaaggattc tggccactgg
     1981 ttataatggt gcaccaagtg gtttagctca t
//
LOCUS       DAOSOL010000268         2008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_56267_length_2008_cov_2.670251, whole genome shotgun sequence.
ACCESSION   DAOSOL010000268 DAOSOL010000000
VERSION     DAOSOL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56267_length_2008_cov_2.670251"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            860..1639
                     /locus_tag="PK171_06085"
     CDS             860..1639
                     /locus_tag="PK171_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05792.1"
                     /translation="MKSKFGSIFKGELKNSLGSIILLLSLVILWDLYLYLRRDSWDIT
                     LVFVLSFLPIIFLPFYALVSGFYMLREEWRKKTITHLLSLPVKGITLTSIKLLTIWIE
                     TVIFIVVIFIGVIMFSKIALLEPIPNQVLWQLGIILSIISILVAILSQFAYLVGRVFR
                     YGGWLISIWTFLVTGWAIIRYSGLLVPYLSFVPNFQLNGWFLSGIWQYLGDVTALQAI
                     KIHGPTTLAFFLSFFVIFLLGSWILEKYVIVPTGEIKHESE"
     gene            1626..1799
                     /locus_tag="PK171_06090"
     CDS             1626..1799
                     /locus_tag="PK171_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05793.1"
                     /translation="MKASKVLLLLLIGLTFLFIFDYSRGGIHKIGPQLSQIIILPAPA
                     DNIKALDDDWFGK"
BASE COUNT          578 a          372 c          378 g          680 t
ORIGIN      
        1 ataatgaaaa acttcccatt cttgatatca agctattaca acacacctta aaagaacaaa
       61 atgtaatttt gtaaccaatc atagttttta aaaatgaagt aaggggtgag ttgtgagcag
      121 tgagaaacaa cttgttcatt cttatcaccc cctctcacac tcgccccttc agttttttgc
      181 taggaggaaa aacacttttt ccttttttct tcttccctgg tgggagagga ttaaggtgag
      241 agggtaactc attttcatcc tcatttggtg ctgttgaata acatgaaggt ctatcctgta
      301 aatatcatca ataaattctt taatccgtca tcctgaggct tcgtttttcg aagccgtgag
      361 gatctcatct gacatcgaag gtgtcatcct gagcggtgct ttcccgcgcg aggatctcat
      421 cttttatatt tctttgtttt ttaaaatatt ttaaaggatg agattatcac gtcgtctgac
      481 caaaaaaggt tggcctcctc gtaatggcag ttataagaaa aatatttttc tttatcatct
      541 caaaccgtga agcagtataa ggggcctttt tccagaaaga gttatggaaa gagagaaaca
      601 atttccatat ttatcggatc acgccattca gaatgggaat ggattattga acatttccga
      661 taattttata gaatcatatt cagatattat tcaaaatagg ttaaactatt accttaagtt
      721 ggaaataaaa agcaataggg gtgcttcatt atagcacccc tatttttaac ctatctggtg
      781 gtttggttaa tcaataccaa aagctcaaaa ggttaagaaa aatttaaatg agtgttgtct
      841 taaaacaccg ggtgaagaaa tgaaatcaaa gtttggctca atattcaaag gtgaactaaa
      901 gaattcttta ggaagcataa ttctccttct ttctctggtt atactctggg acctgtatct
      961 ctatctccgt cgggattcat gggatataac cctggtcttt gttttaagtt ttcttcctat
     1021 cattttcctt cccttttatg ccttggtcag tgggttttat atgcttcgag aagaatggcg
     1081 gaagaaaact attacgcacc ttctttcgct gccggtgaaa gggattacct tgaccagcat
     1141 taaactactt acgatatgga tcgaaacggt tatcttcatt gtagtgatat ttatcggagt
     1201 cattatgttt agtaagattg cccttttaga acccattccc aatcaagttt tgtggcagct
     1261 cgggataatt ttatcgatca tttctatcct ggtggcaatt ttaagccagt ttgcctattt
     1321 ggttgggcgt gtatttcgtt atggagggtg gttaatcagc atctggacct tccttgtgac
     1381 cggttgggct ataatccgtt attctgggct gcttgttcct tacctttcct ttgttcccaa
     1441 cttccagtta aatggttggt ttctttctgg aatttggcag tatttaggag acgtcaccgc
     1501 tcttcaagcc atcaaaatcc acggaccgac cacgctggca ttctttctga gctttttcgt
     1561 catctttctg ttgggttcct ggattttgga aaaatatgtc attgtgccaa cgggggaaat
     1621 aaaacatgaa agcgagtaaa gtattactgc ttctattgat cgggctgact ttccttttta
     1681 tatttgatta ttcccgaggt ggaattcata aaattggacc tcaattaagc caaataatta
     1741 tccttccagc cccagccgac aatattaagg ctttggatga tgattggttt gggaagtaaa
     1801 taagtttcat ttagcggtaa aaataataaa ttgaaatcct ctaaatctgc aattttaaat
     1861 acaaatgaac tttgtgggta atgatcactg ataagcgaat aaaaaccgag taggataagg
     1921 ggatgaatac ttcatcctca tgtaactaca atttaattga atcatcctca atccaaaaac
     1981 tccctatctt tccataacat taaaatat
//
LOCUS       DAOSOL010000269         1992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_56843_length_1992_cov_3.739288, whole genome shotgun sequence.
ACCESSION   DAOSOL010000269 DAOSOL010000000
VERSION     DAOSOL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56843_length_1992_cov_3.739288"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PK171_06095"
     CDS             complement(<1..674)
                     /locus_tag="PK171_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOT05794.1"
                     /translation="MTNDEYFQNIKQRAEAAYNKKAALGPDIDLSEFSVCSPHERVDS
                     IESISRSMKEAALYAGIDLLDSGNAATYVQVDRSAVFERIQKAFQGKLEIMSISQAIE
                     KYPEVRNYYWNLIPVDFDKYTAYTELCQTEGYFIRIFAHQQINVPLQACLLMSEEASV
                     QSVHNLIILEEGAQANINTGCASVKGNKNGLHIGVSEFYIGKNAQLTFTMVHNWGENF
                     HVRPRTV"
     gene            complement(671..1423)
                     /locus_tag="PK171_06100"
     CDS             complement(671..1423)
                     /locus_tag="PK171_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05795.1"
                     /translation="MENAGEIMLQIEHLKVEVGNEVILKDVNLTIGEGEIHILLGPNG
                     AGKTTLLMAIMGMPGYTILDGRIIFQGKDITSLSVEERSQLGIGMAFQKMPTIPGVQL
                     QTLGNLIIEKHKNSLSVDEVSKKLNCGSLLSRSIGQGFSGGEAKRAELFQLLLHRPIF
                     SMIDEPESGVDLDNISLVGNALQELFERQLVKTKKRSGLIITHTGFILDYLNAEMGHV
                     LMNGRIICQGNPRDIFSDIKLHGYEACARCER"
BASE COUNT          592 a          406 c          353 g          641 t
ORIGIN      
        1 acggttcggg gacgaacatg aaaattttca ccccaattat ggaccatagt aaaggtcagt
       61 tgagcatttt ttccaatata aaattcactg accccgatat gcaaaccgtt tttgtttcct
      121 tttaccgatg cgcatcccgt attgatgttg gcttgagctc cctcttcgag gatgattaaa
      181 ttgtgaacgc tttgtacact ggcttcttct gacattaaca aacaagcttg aaggggaaca
      241 ttaatctgct gatgagcaaa aattcggata aaataccctt cggtttggca tagttcggta
      301 taggcagtat atttatcaaa atcaactggg ataagattcc agtaataatt ccttacttca
      361 ggatattttt caattgcctg gctaatgctc atgatttcca actttccctg aaaagccttt
      421 tgtatccttt caaagacagc tgaacgatca acttgaacat aggttgcggc atttccggaa
      481 tccagcaaat caatgcctgc atataaggca gcctctttca ttgatcggga aatgctttcg
      541 atactatcga ccctttcatg gggagagcat accgaaaatt cgctcagatc aatatccgga
      601 cctaatgccg cttttttgtt ataagctgct tctgctcttt gtttgatatt ttgaaaatat
      661 tcatcattgg tcatcgttca cacctcgcac aggcttcata accatggagt ttaatgtcag
      721 agaaaatatc tcggggattc ccttgacaaa ttattcttcc attcattaaa acatgcccca
      781 tttcagcatt taaatagtct aaaataaaac cggtatgggt aataattaat ccagatcgtt
      841 ttttagtttt aactaattgc cgttcaaaaa gttcttggag ggcgtttcct accagagata
      901 tattatcaag gtcaactcct gattcgggct cgtcgatcat agaaaaaatc ggacgatgca
      961 aaagaagttg aaaaagctcg gctcgtttgg cttctcctcc agaaaatcct tgaccgatac
     1021 ttcggcttaa aagagaacca caatttaatt ttttcgaaac ctcatcaacg gatagagaat
     1081 ttttatgttt ttcaataatt aagttcccga gagtttgaag ttggacccct ggtattgtcg
     1141 gcattttttg gaatgccatt cctatcccca gctgggaacg ttcctcaaca ctcagagaag
     1201 ttatgtcttt tccctgaaaa attatccgcc catctaaaat agtatatcct ggcatcccca
     1261 tgatagccat caataaagtt gtttttccag ctccattcgg accgagtaag atatgaattt
     1321 caccttcacc aatggtaaga ttcacgtctt tcaaaatgac ctcgttacct acctcaactt
     1381 ttaaatgttc aatctgaagc ataatttcac ctgcattctc caaaagtctc tttttttcat
     1441 tatatcatgg aaccggatcc taactctttt cgaaatcaat catctgaaat tcttagttca
     1501 aagacattaa attctccaag tgcaaaagtg cagatatttt tgccattctg aatccagttg
     1561 aggatagagg taaaattagg accattacta ataaccggtt ggaatttatg ggttgaaaaa
     1621 attgtagaaa ttcactttta tcataaaaaa gagcttcata aagatctatt gaagtaactt
     1681 tatgttattg atattgctct cgcaatatgg gaaaatagaa aaaagcttga cttcttaggt
     1741 taattctaaa gccgcttcgt cataataaaa gattgaactc caataaatct atcctgaaaa
     1801 tactatcaaa tgaattcccc cattgagggg ggatgcaggg ggggtgtgcc tttcatcagt
     1861 catcctgagg cttcgtattt tgaagccgtg aggatctcat ctgatgccga tagtgtcatc
     1921 ctgaggcttc gtattcagaa gccgtgagga tctcatcttt ttaggttttt tattcttcat
     1981 aaatacttta aa
//
LOCUS       DAOSOL010000270         1989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_56962_length_1989_cov_4.263185, whole genome shotgun sequence.
ACCESSION   DAOSOL010000270 DAOSOL010000000
VERSION     DAOSOL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56962_length_1989_cov_4.263185"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="PK171_06105"
     CDS             <1..661
                     /locus_tag="PK171_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT05796.1"
                     /translation="GFMRRYDPAIRKAYQMVQDGIIGDLVVIKSTGRGPGLPPAWIWD
                     RSKSGGMLAEVSAHDIDSVLWFTGKKPERIYMEAKNFKSPQASEKFPDFYDHYIVTVT
                     FQDGPLGSIDGGCPVGYAYDGRMEILGNKGMIRIGTTEGNGPVLMTLDKKAICDTHDA
                     WRNRFKEGYLEEMKSFIRCIQKDEKPEPSVVDGFRAVQVVEYAHRSIKQQKPINFDGE
                     W"
     gene            665..>1989
                     /locus_tag="PK171_06110"
     CDS             665..>1989
                     /locus_tag="PK171_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT05797.1"
                     /translation="MTYLIGCDLGTTATKTALFNDKGELIVVEKVDSNIIYGDDRSVT
                     QDPDEMFQSVIQSVKKVMEKSGISPNQVAAIALDSQMAGIMGVDDRGEAATPYDSWLD
                     FRSADYAEMMKKEAEQLVIRQSGMGPSINHGPKILWWKHERPEIYRKIKKFTVPSSWI
                     TQKLAGLSGEETYLDYTNLHFTCFGDLRNLRWDKEILQIFDVEEDKFPRIVDPCLQIG
                     KLQQPWAKEMGLLAGIPLIAGCGDQAANALGAGVSEPGIAFDVAGTASCFSLFVDEYN
                     PDVKYKALMLAQSVLKDFYYVMAYINGGGMDLEWAKKELFREWADSRDAFQLMAQEIE
                     QNAPKPSGVIFIPHLRGRNFPTQPWLRGVFAGFSWDHRRENLYRAILEGIAFEYAFYL
                     KVIRELIPNLTFKEVRVIGGGAKSPLWNRIKASILKVPYVELNRQEYAV"
BASE COUNT          608 a          318 c          488 g          575 t
ORIGIN      
        1 cggttttatg aggcgatacg atccggccat acgaaaagcc tatcaaatgg ttcaagatgg
       61 aattattgga gatttggtgg tgattaaatc gaccggtcga ggaccagggc ttcctccggc
      121 ctggatttgg gatcgatcaa agagtggtgg aatgttggcc gaagtttctg ctcacgatat
      181 tgattcggtt ttatggttta ccggaaaaaa accggaaaga atttacatgg aagcaaaaaa
      241 ttttaaatca ccccaagcca gcgagaaatt tcctgatttt tatgaccatt atattgtgac
      301 tgttactttc caggatggtc cattaggatc aattgatggt ggttgtccgg ttggatacgc
      361 ttacgatgga aggatggaaa tattaggtaa caagggcatg ataagaattg gaaccaccga
      421 agggaatggt ccggtattaa tgactttgga taaaaaagcc atttgtgaca cccatgatgc
      481 ttggagaaac cgttttaaag aaggttattt ggaagaaatg aaatcgttta ttcgatgtat
      541 acagaaggat gaaaagccgg aaccatcggt ggtagatggt tttcgggcgg tgcaagtggt
      601 ggaatatgcc caccgatcaa taaaacaaca aaaaccgata aattttgacg gggagtggtg
      661 aaaaatgact tatttgattg gttgtgactt gggaacaacg gctacaaaaa cagctctttt
      721 taatgataag ggtgaattga tagttgttga gaaggttgat tccaatataa tttatggcga
      781 tgatcggagt gttacccagg atcctgatga aatgtttcaa tcggttatac aatcggtcaa
      841 gaaagttatg gagaagagtg gtatttctcc gaaccaagtt gcagcaatag cacttgatag
      901 tcaaatggca ggaattatgg gagttgatga tcggggtgaa gcagcgaccc cctatgattc
      961 ttggttagat tttcgttcgg cagattatgc tgaaatgatg aaaaaggaag cagaacaatt
     1021 agttattcgt cagagtggca tgggaccaag tatcaatcac ggtccaaaaa ttttatggtg
     1081 gaaacatgaa agaccagaaa tttatcgaaa gattaaaaaa ttcaccgttc cttccagttg
     1141 gataacgcaa aagttagctg ggttatctgg tgaagaaact tatttagatt ataccaattt
     1201 gcactttacc tgttttggag accttcgcaa tcttcgttgg gacaaagaaa tccttcagat
     1261 atttgatgtt gaagaggata aattccctcg aatagttgat ccttgtctgc aaattggtaa
     1321 gttgcaacaa ccatgggcaa aagaaatggg gttgttagct gggattcctc tgatagctgg
     1381 ttgtggtgat caagcagcaa atgccttagg agctggagta tctgaaccag gaattgcttt
     1441 tgatgttgcc gggactgcat catgctttag cttgtttgtc gatgaataca atcctgatgt
     1501 aaaatataaa gccctcatgt tagcccaatc tgttttaaaa gatttttatt atgttatggc
     1561 ttatattaac ggtggtggaa tggatttaga atgggcaaaa aaagagctct ttcgggaatg
     1621 ggctgattcc cgcgatgctt tccaattaat ggctcaggaa atagaacaaa atgcaccaaa
     1681 accttcggga gttatattta ttccccactt acgaggaaga aattttccca cccaaccctg
     1741 gctacgagga gtttttgctg gttttagctg ggatcaccgg cgtgaaaatc tataccgagc
     1801 gatattggag ggcatagctt ttgaatatgc tttttactta aaagtcattc gagaattgat
     1861 acccaacctt acttttaaag aagttcgggt gattggggga ggagcaaagt ctcctctttg
     1921 gaataggatt aaagcgagta ttttaaaagt accctatgtt gaattaaatc gccaagaata
     1981 tgcagtatg
//
LOCUS       DAOSOL010000271         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_57115_length_1985_cov_3.321762, whole genome shotgun sequence.
ACCESSION   DAOSOL010000271 DAOSOL010000000
VERSION     DAOSOL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57115_length_1985_cov_3.321762"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PK171_06115"
     CDS             <1..485
                     /locus_tag="PK171_06115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05798.1"
                     /translation="GLITIDSLLTGNLTAFLDALKHLMLPAFSLALGGMFQEARITRS
                     SMLENSKKDYVSAARSYGIPERTVIFKYLFKPSIIPTVSILGLDFASLMGNAFLVELL
                     FNWPGISRYGINVMLRKDVDAIIAVIMVLGIVFTLINIIVDIIVAYLDPRIRLVERGD
                     "
     gene            491..1366
                     /locus_tag="PK171_06120"
     CDS             491..1366
                     /locus_tag="PK171_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05799.1"
                     /translation="MNLSPKKSKRTKSGKAWYKFSRNPLSVVGLVIVLFVIFSALLAP
                     WVTPYPAHSGKYVNYGEANFSPSIQHLFGTDVFGRDVFTRTIYAFRSSLIMGIVVLAI
                     VVPIGVTLGLLAGYFKETWIDVLIMRITDIFLSVPPLILALAITSVLKPNLTNAMLAV
                     SVMWWPWYTRLVYGMATSLRNEYFVQSAELLGASKFHVLFKEILPNCLSTILTKMTLD
                     MGWVILIGASLSFVGLGEQAPKAALGSMVADGAKYLPEYWWISVFPGLAIMLIVLGFN
                     LLGDGISDLFSMEEA"
     gene            1369..>1985
                     /locus_tag="PK171_06125"
     CDS             1369..>1985
                     /locus_tag="PK171_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05800.1"
                     /translation="MSQLILDIKNLTVNFRVYGGYLKVLNEVNFSVVQGEKVGLVGET
                     GCGKTTTMKTVMGILPQPPGKIAQGEIFFQGKNVLTMKKSEIEKMRQTGISMIFQDPT
                     AALNPVFTIGQQLYDVVQHSQSSDDGQSRLSAHEVSIRALQDVSLPDPKRILDNYPLQ
                     LSGGMRQRVCIALAIARAKQLLIADEPGTSLDVTIQDQILRLLNQ"
BASE COUNT          526 a          430 c          396 g          633 t
ORIGIN      
        1 ccgggctcat aaccattgac agtttgctca ccggaaatct aactgccttt ttagatgctt
       61 taaaacatct aatgcttccc gcgtttagcc ttgctttagg aggaatgttt caagaagctc
      121 gtatcacccg ttccagcatg ttggaaaact caaaaaaaga ctatgtctca gctgctcggt
      181 cctatggtat cccggagcga acggtaatct ttaaatacct tttcaagcct tccattatcc
      241 caaccgtctc tatccttggt ttagattttg catctttaat gggaaatgct tttttagtcg
      301 aacttctgtt taactggccg ggtatttccc gttacggaat taatgtcatg cttcgcaaag
      361 atgttgatgc cataatcgcg gtaattatgg tcctaggaat tgtttttact cttatcaaca
      421 ttattgtcga tatcattgtt gcctatctcg atccacgaat ccgattagtt gaaagaggag
      481 attaattgca atgaatctat ccccaaaaaa atccaaaaga accaaaagcg gtaaagcttg
      541 gtataaattc tctcggaatc ctctctcggt tgtgggttta gtaattgtcc tttttgtaat
      601 ttttagtgct ttgcttgcgc cttgggttac tccctatcct gctcattccg gaaaatacgt
      661 aaactatggg gaagccaatt tttctccatc gatacaacat ctatttggta cagatgtttt
      721 tggccgtgat gtgttcaccc gaaccatcta tgcttttcgc tcatcattaa ttatgggaat
      781 tgttgttctc gcaatcgttg tcccaattgg agttaccctt ggtcttttag ctggttattt
      841 caaagaaacc tggatagatg tcttgatcat gagaataacc gatatctttc tttcggtgcc
      901 tcctctaata ctcgccttgg ccattacttc agttcttaaa cccaatttaa ccaatgctat
      961 gttagccgtt tcagtaatgt ggtggccgtg gtatacccgg ttggtttatg ggatggccac
     1021 ctctttacgg aatgaatatt ttgttcaatc agcagagcta ttgggagcga gtaagtttca
     1081 cgtccttttt aaagaaatac tccccaattg tctatcaacc attttgacca aaatgaccct
     1141 tgatatgggt tgggttatcc ttatcggagc atcactttct tttgttggat taggagagca
     1201 agctcctaag gcagccctgg gatccatggt tgctgatgga gcaaaatatc ttcctgaata
     1261 ttggtggatc tcggtattcc cgggtttagc tatcatgctg attgttttag gattcaacct
     1321 ccttggtgac ggcatcagcg atttattttc aatggaggaa gcctaaacat gtctcaactt
     1381 attcttgaca tcaaaaatct aaccgttaac tttcgtgttt atggtgggta tttaaaagta
     1441 ttaaatgaag ttaatttctc ggttgtacaa ggagaaaaag taggtctcgt cggtgaaact
     1501 ggctgtggaa aaactaccac catgaaaacc gtcatgggta ttcttcctca acctccaggg
     1561 aaaatagccc aaggtgaaat ttttttccaa ggaaaaaacg ttcttaccat gaagaaatcc
     1621 gagattgaaa aaatgcgtca aactggaata tcaatgatat tccaagatcc aaccgccgcc
     1681 ctcaatccgg tttttaccat cggacaacaa ctttatgatg tggtgcaaca ttcccagtca
     1741 agcgatgatg gccaatctcg cctttctgcc catgaagtct cgatacgagc actccaggac
     1801 gtttctctcc ctgatccaaa acgcatatta gacaattatc ctttgcaatt aagtgggggc
     1861 atgcggcaac gagtctgcat tgcccttgcc atcgccaggg ccaaacagtt actcatagct
     1921 gatgagccgg gaacctcttt agatgtgact attcaagatc agattttaag attgctcaat
     1981 caaat
//
LOCUS       DAOSOL010000272         1980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_57333_length_1980_cov_2.521039, whole genome shotgun sequence.
ACCESSION   DAOSOL010000272 DAOSOL010000000
VERSION     DAOSOL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57333_length_1980_cov_2.521039"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PK171_06130"
     CDS             complement(<1..398)
                     /locus_tag="PK171_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HOT05801.1"
                     /translation="MKKDSENSIRILNNIVNGLYSSTVTNQPDALPLNTKEKVLRVAV
                     GSDHGGFEAKEKLKVFLKELGYRVTDVGTYNTESVDYPDFALKVALKVKSGDCERGIM
                     IDGAGIGSSMVCNKVRGIRAALCYDLKTIV"
     gene            complement(398..682)
                     /locus_tag="PK171_06135"
     CDS             complement(398..682)
                     /locus_tag="PK171_06135"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOT05802.1"
                     /translation="MILAKVIGQVVSTMKLDIFKGFKLLLVKPVDIEEKPKGKSLLAL
                     DTVQAGVGDIVLVMDEGNSARTVLENTTAPIRSIIVGIVDEIQTTQRGEK"
     gene            complement(679..957)
                     /locus_tag="PK171_06140"
     CDS             complement(679..957)
                     /locus_tag="PK171_06140"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOT05803.1"
                     /translation="MKIGKVIGTVVCNQKVDSLNGIKLLLIQPLNETLKEEGTPVVAC
                     DAVQAGLNDIVFYEGGREAALGLTNWFNPSDLTVMGIIDTLYTEEDIK"
     gene            complement(979..>1980)
                     /locus_tag="PK171_06145"
     CDS             complement(979..>1980)
                     /locus_tag="PK171_06145"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOT05804.1"
                     /translation="STNPTTSVINNTISMIAGGNTVVFHPHPNAKKCSCTAVSLINQA
                     IVDAGGPENVITALQNPTLETAQTMMKHPKIRLLVVTGGPGVVRVAMNSGKKVIAAGP
                     GNPPCVVDETADIPKAGRDIVRGAGFDNNIICICEKEILAVSSIADRLKEEMKKNGAY
                     ELNKSQIDKLTPLIIEKPGGPGQEGFPNKKYVGKNANLIAKEIGLNLPDEIRLLLCEV
                     DSNHPLVWTEQLMPVMPLARFNSVEEAIHFSVQCEHGFRHTAIMHSKNIAHLSAMAKL
                     MNCSIFIKNGPSYCGLGFEGAGFTSFTIASPTGEGLTRARTFTRERRCTLVDYFRIV"
BASE COUNT          572 a          450 c          328 g          630 t
ORIGIN      
        1 ttaacgatag tttttaagtc gtaacatagt gccgctcgaa tcccccttac cttgttacaa
       61 accatggatg aaccaattcc agctccatca atcataatcc ctctttcaca atcaccactt
      121 ttcactttta aagctacttt gagagcaaaa tcaggatagt caacactttc agtattgtaa
      181 gtcccaacat cggtcactcg ataacccaac tcttttaaaa aaactttcag tttttctttg
      241 gcttcaaatc ctccatgatc ggacccaact gcaactcgta aaactttttc cttggtattt
      301 agagggagag catctggttg attggtaacg gttgaagaat ataaaccatt aactatgttg
      361 ttcaagatac gaatagagtt ttccgaatct tttttcatta tttctctcct ctttgggtgg
      421 tttgtatttc atcaactata cccacgataa ttgaacgaat tggggcagtt gtattttcaa
      481 gaactgtacg cgctgagttc ccttcatcca ttaccaatac tatatctccc actccagctt
      541 gaacagtatc aagcgctaaa aggctttttc cttttggttt ctcttcaata tcaaccggtt
      601 taactaacaa caatttgaaa cctttaaata tatctaattt cattgttgaa acgacttgtc
      661 caattacttt cgctaaaatc attttatatc ttcctcagta tataaagtat caataattcc
      721 catgactgtt aaatcggagg gattaaacca attggttaaa cccagagcgg cttctcttcc
      781 tccttcataa aaaactatat cattcaaacc tgcctgaact gcatcacagg caacaactgg
      841 agttccttct tcctttagag tttcgttcag cggttgaatt aacaataact taattccatt
      901 taatgaatcc actttttggt tacaaaccac tgttccaatt acttttccta tcttcatatt
      961 actccctctg acttccgatt aaacaattcg gaaataatca actaaagtac accgacgctc
     1021 ccgagtaaag gttcgcgccc gggtgagacc ttcgccggtt gggctggcga ttgtaaatga
     1081 agtaaaacct gcgccctcaa aacccagtcc acaataactt ggtccatttt taataaaaat
     1141 cgaacaattc ataagctttg ccatagcact gagatgcgca atattttttg aatgcataat
     1201 ggcagtatga cgaaaaccat gttcacattg aactgaaaaa tgaattgcct cctcgacgct
     1261 attaaaacga gctaatggca taactggcat aagttgctca gtccacacca atgggtggtt
     1321 ggaatcaact tcgcatagca acaatcgaat ctcatcagga agatttaaac ctatttcttt
     1381 agcaatgaga ttggcattct tcccaacata ttttttgttg ggaaatcctt cttgacctgg
     1441 accaccaggt ttctcaataa tgagtggtgt aagtttgtca atctgacttt tgttcagctc
     1501 ataagctccg ttcttcttca tttcttcttt taaacgatca gcaatgcttg aaactgctag
     1561 gatttctttt tcacaaatac aaataatgtt gttatcaaat ccagcacccc ttacaatatc
     1621 acgaccggct ttaggaatat cggcagtttc atcaacaaca cagggaggat tcccaggacc
     1681 ggcagcaata actttctttc cactattcat ggctaccctt actactccag gtcctccggt
     1741 tacaaccaat aaacggattt tgggatgttt catcatggtc tgcgctgtct caagggtagg
     1801 gttttgaaga gctgttatga cattttccgg accaccggca tccacaatag cctgatttat
     1861 gagtgagact gccgtgcaag aacatttttt tgcattggga tgaggatgaa aaaccaccgt
     1921 atttccgccg gctatcatgc ttattgtatt attaatgacc gaagtagtgg gatttgtcga
//
LOCUS       DAOSOL010000273         1973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_57611_length_1973_cov_4.148071, whole genome shotgun sequence.
ACCESSION   DAOSOL010000273 DAOSOL010000000
VERSION     DAOSOL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57611_length_1973_cov_4.148071"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(420..1562)
                     /locus_tag="PK171_06150"
     CDS             complement(420..1562)
                     /locus_tag="PK171_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05805.1"
                     /translation="MNKILSSRERVLTALDHKEPDRVPLDLGGYQSGITTIAYEKLKI
                     QMGINRPTKISERNQQLAVIDEEVLKEFGIDTRYVFMKPSASWDPKEGSDEKSTWYFD
                     EWGAQWKKPHTSFYYDPVGAPLKEATIEDLGHYHWPDPNEKSRFENVEKEAQAIYDAG
                     YALGTTVSGVFEQAWYLVGLERIMIEIIENPSFVEALLDQVLVILSQQYSNFLEKVGQ
                     YLTLIEIWEDISSQQGPLTSPNIYRKIIKPRTRDLIQVIKSKTKAKVALHSCGSTSWA
                     IEDFIEIGIQVLNPVQVSAAHMNTKELKKKYRDSISFWGGIDTQRVLPRGTTQEVEDE
                     VKRRIDDLGERGGYLLAPVHNIQPDVPPENIIAMYQTGLEYGRYHN"
BASE COUNT          514 a          429 c          384 g          646 t
ORIGIN      
        1 ttccgataga aattgtacct gtgggtgggc gcctgcttga acacatgctt cataaacggc
       61 ttgaaccaag ggataggttt caacttccgt catagcaatc ataacccgtt ctttcggctg
      121 aacgctggtt gaataattga ccaacaactt tcccagttct ttccaacgct tatccatatt
      181 gttttccttt tatgaattct tttttttatc gttcaattat tttaccatag gtatttcttt
      241 atttattctt agattgataa ttgatactat caataatcca aagataaggg cgtagactaa
      301 tgaaaccggt aaaataatta cgggacaaca aaatcattga aatctcgtat gaaggactgg
      361 ttattgaacc aggtttgcct ttcaatcggt tgaaaaattg cgctaaaaga gaataccaat
      421 tagttatgat atcttccata ctccaatcca gtctgataca tggcaatgat attctcagga
      481 ggaacatcgg gctggatatt gtgaactggt gccaaaaggt atcctcctct ctcccccagg
      541 tcatcaattc gtcgtttcac ttcatcctcg acttcctggg tggtcccacg ggggagaacc
      601 cgttgagtat caattccacc ccagaaacta attgaatccc gatatttttt cttcaattcc
      661 ttggtgttca tgtgagcagc actcacctga accggattta agacttgaat gccaatttca
      721 atgaaatctt caattgccca actggtgctc ccacagctat gaagggcgac tttggccttg
      781 gtttttgact tgatgacctg aatcaaatcc cgagtccggg gcttgatgat ttttcggtag
      841 atattgggtg aggtcaatgg tccttgctgg gaactgatgt cttcccaaat ttcaatgaga
      901 gtgagatact gaccgacctt ctccaggaaa ttgctgtatt gttgggaaag gatgaccaga
      961 acttgatcca gtaaggcttc aacaaaggag ggattctcga tgatttcgat catgattcgt
     1021 tctaaaccga ccagatacca ggcttgttcg aaaacccccg ataccgtggt gccaagagca
     1081 tagcctgcat cataaatcgc ttgggcttct ttttcgacat tttcaaaacg gcttttttca
     1141 ttgggatccg gccagtggta atgaccgagg tcttcaatcg ttgcttcttt gagaggagct
     1201 ccgaccgggt cataataaaa gctggtatgg ggttttttcc attgagcacc ccattcatca
     1261 aaataccagg ttgacttttc atcactgcct tctttagggt cccaggaggc ggatggtttc
     1321 ataaaaacat agcgggtgtc gataccaaac tcttttaaaa cttcttcatc gatcaccgcc
     1381 agttgttgat tgcgttctga gatcttagtg ggtcgattga ttcccatttg gattttgagc
     1441 ttctcataag caatggtggt tatacctgat tggtatcctc ccaggtccaa tgggacccga
     1501 tcgggttctt tatggtccag tgcagtaaga actctctcac gagagctaag aattttgttc
     1561 atattttttc ctccttcatt tggacagtta accttttgaa attgatatca ttttcttaaa
     1621 tatgttagtt tcaagctcag atcagcaatt cttttcagca aaaccgatag taatctataa
     1681 tactctatcc tttttaaaga taactttcat aatgacccaa atttaaggct taaattcttg
     1741 ccttcgaata ttacaacttt actctttctt agtacattgt atcctcttct tctgatttcg
     1801 gaaaccggaa acccttccta tttactttta aaactttctt tgtttaaggt ttctatcctg
     1861 aaaatactat caaatgaatt ccctctttga agagttaggg gggtgtgccc tttattcatc
     1921 atcctgagcc ctcgtttttc gagggcgtga ggatctcatc aacccaatcc gtc
//
LOCUS       DAOSOL010000274         1969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_57781_length_1969_cov_4.112330, whole genome shotgun sequence.
ACCESSION   DAOSOL010000274 DAOSOL010000000
VERSION     DAOSOL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57781_length_1969_cov_4.112330"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PK171_06155"
     CDS             <1..322
                     /locus_tag="PK171_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05806.1"
                     /translation="LLDQVDISYQKALDLAATNLPEDSELIFQLIKDVTNYYPQSQTM
                     QKKIEVLYQSIFAKQAGVKTEESANTTQIEIKITASSIESGSVDHRNHDESSPRFYID
                     TDFE"
     gene            complement(338..424)
                     /locus_tag="PK171_06160"
     tRNA            complement(338..424)
                     /locus_tag="PK171_06160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(388..390),aa:Leu,seq:caa)
     gene            498..1436
                     /locus_tag="PK171_06165"
     CDS             498..1436
                     /locus_tag="PK171_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOT05807.1"
                     /translation="METSKNLILLLDGSSLIYRAFYAMPRFTSSQGKPTGAVLGFGNM
                     LLRLIEDFNPFSLITTFDHPQKTFRHQLTEEYKAQRKPMPDELIPQVQLVKDLIDSFS
                     LKWIEVPGFEGDDIIGSLRHQIPEQFTTAMVSSDLDLLQLVNEKTILLQPVKGVTQLK
                     IIDKNTLIDQYGLTPQQIIDFIALTGDPSDNIPGLPGIGEKTALDLLKRYHNWQGILR
                     HHDELPVRIQKAIQEFIEQVELSLQLVTIIDTIPLDINFQSWNFQQVQWKSLFTLLDE
                     LGLVRFKERLMKKRNEIYQKTLPFFSNNPKKRGEEL"
     gene            1483..>1969
                     /locus_tag="PK171_06170"
     CDS             1483..>1969
                     /locus_tag="PK171_06170"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT05808.1"
                     /translation="MDIIMVSSEAYPFAKSGGMGDVVGALTKYLPNNDFSIKLFLPAY
                     SSLLSEFQFNPEQDITFSFGESMLKASYLTRNEKNLQIIAVAGEDYFNREKMYGFGDD
                     LKRFIFFSRAVFEYIVRTQKEAFILHCHDWESALLCAYTKMYWPSYRKKPKKVIFTIH
                     NL"
BASE COUNT          617 a          388 c          386 g          578 t
ORIGIN      
        1 tcttttagat caagtcgata tcagttatca aaaagctctt gatttagccg ccacgaacct
       61 gcctgaagat tcggagctga tctttcaact cattaaagac gttacaaatt attatcccca
      121 aagtcagacc atgcagaaaa aaattgaagt cttataccaa agcatctttg caaagcaagc
      181 tggcgtgaaa actgaagaat cagccaatac aactcaaata gaaatcaaaa ttacggcaag
      241 tagcattgaa agtggatcag tagatcatcg gaatcatgat gagtcaagcc caagatttta
      301 cattgatacc gattttgaat gattcagttg gaacacgtgg tgccgaaggc gggagttgaa
      361 cccgcacgag ccggagctcg ctagattttg agtctagtgc gtctgccgat tccgccactt
      421 cggccctcgt tgtagaaatt atagtcaatt caatgctttt gtcaacctgt ttctattcga
      481 acaaaaaagg gtgaatcatg gaaacctcaa aaaatttaat actattatta gatggaagct
      541 cgctaattta tagggctttt tatgccatgc cacggtttac ctcgtctcaa ggtaagccaa
      601 ctggtgcagt tttgggattt gggaatatgt tacttcggtt aattgaggat tttaacccct
      661 tctccctgat tactactttc gatcatcctc aaaaaacttt tcgccatcag ctaaccgaag
      721 aatacaaagc ccagcggaaa ccaatgccgg atgagttaat accccaagtt cagttagtca
      781 aggatttaat tgattctttt agcttgaagt ggatcgaagt tcctggattt gaaggtgacg
      841 atataatcgg aagtcttcgt caccagattc cagaacaatt caccaccgct atggtttcat
      901 cagatctcga tttgttgcag ttagtgaatg aaaaaactat tcttcttcaa ccagtaaagg
      961 gtgtaactca attaaaaata attgataaaa atactttaat agatcagtat ggattaactc
     1021 ctcagcagat aatcgatttt atagctttga cgggtgatcc ctccgataat attcccggac
     1081 ttcccggtat tggagagaaa accgctcttg atcttttaaa acggtaccac aattggcagg
     1141 gaattctgcg ccatcatgac gagcttccag tgcgaatcca aaaagctatt caggaattta
     1201 ttgaacaagt tgaactcagc ctccagttgg ttaccattat cgataccata ccactcgata
     1261 tcaattttca gtcttggaat tttcagcagg tccaatggaa atctttattt accttgttag
     1321 acgaattagg attggttagg tttaaggaaa gactgatgaa aaaaagaaat gagatttatc
     1381 aaaaaactct cccttttttt tctaataacc caaaaaaaag aggggaggag ttataattta
     1441 aacccaattt ttaatcaatt gaggggaaca ggaggacaat gaatggacat catcatggta
     1501 tccagcgaag cgtatccctt tgctaaaagc ggaggaatgg gtgatgtggt tggagcgctc
     1561 accaaatacc tcccgaataa tgatttttca ataaagcttt ttttacccgc ctattcgtca
     1621 cttcttagtg aatttcagtt taatccggaa caagatatca ccttttcctt tggagagagc
     1681 atgttgaagg cttcctacct cacccggaac gagaaaaact tacaaatcat agcggttgca
     1741 ggtgaagatt attttaaccg ggaaaaaatg tatggttttg gcgatgatct gaagcgcttc
     1801 atttttttct ctcgagcagt gtttgagtat attgttagaa ctcaaaagga agccttcatt
     1861 cttcattgtc acgattggga aagtgcttta ctctgtgctt acaccaaaat gtattggcca
     1921 tcctatcgga aaaaaccgaa aaaagttatc ttcaccatcc ataatttag
//
LOCUS       DAOSOL010000275         1939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_59062_length_1939_cov_4.176752, whole genome shotgun sequence.
ACCESSION   DAOSOL010000275 DAOSOL010000000
VERSION     DAOSOL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59062_length_1939_cov_4.176752"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PK171_06175"
     CDS             <1..1112
                     /locus_tag="PK171_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT05809.1"
                     /translation="WLGIFLAILLLVSVPVFGQDTFKIGANLEMTGSVAAYGQMINEG
                     VDLIMDIVGTEVLGKQIEYVLVDNKSDKVEAANAATRLIDKEKVVAIIGPAISGSMLS
                     AGPICEEKKIPQISATSTNPLVTQDKKYSFRATFIDSYQGLAAAQYAYVDLGAKSVAI
                     LSDIAQDYCVALGNFFKDAYEKLGGEVLTVQHCKSGDQDFSAQLTTIMNLNPDVIYLP
                     NYYTEIALICRQARDLGYEGKMLGGDGSDAPELVDIGGDAVEGFYFTAHGDTEKALTE
                     IGQKYFDAYRAKFNKEPGVFGALAADCYIIILDAIKRANSFEPEKVAAAIEDTKDLEV
                     TTGKITIEKGDALKPVVVKQVEGGKAKLLGTIEVK"
     gene            1244..>1939
                     /locus_tag="PK171_06180"
     CDS             1244..>1939
                     /locus_tag="PK171_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT05810.1"
                     /translation="MSFTQFFQQLMNGFSLGSLYALLAIGYTLVYGILSLINFAHSDV
                     FMLGTYFAFYMIAIFMLPWWLSVIISVVLCAIVGMTIERVAYRPLRSAPRISALITAV
                     GVSYFLENLGLVVLGARPKGFPRPDLMKNVYVIAGAKIQGVSFWVPIISIGILMVLLY
                     IVYKTKVGMAMRAVAKDMETTRLMGVNVDQIILYTFALGSALAAAGGVMWAAKFPQIN
                     PLMGIFPGWKAFTA"
BASE COUNT          538 a          344 c          435 g          622 t
ORIGIN      
        1 catggttggg aatattttta gcaattcttt tattagtatc ggttccggtt tttggccaag
       61 atacatttaa aattggggca aacctggaaa tgaccggttc agtcgctgct tatggtcaaa
      121 tgatcaatga gggtgttgat ctgatcatgg atattgttgg aaccgaagta ttaggaaaac
      181 aaatcgaata tgtcttggtt gataataaaa gtgataaggt agaagcggct aatgcagcta
      241 cccgcttaat cgacaaggaa aaagttgttg cgattatcgg cccagcaata agtggaagta
      301 tgttatcggc cggtccaatt tgtgaagaaa aaaagatacc ccagatttca gcgacctcta
      361 ccaatccttt ggtcactcag gataagaaat attctttccg ggcaactttt atcgattctt
      421 atcaagggtt agctgccgct cagtatgctt atgtggattt aggtgcaaaa tctgtggcaa
      481 ttctatccga tattgcccag gactactgtg ttgctctcgg gaacttcttc aaagatgcct
      541 atgaaaaatt aggaggagaa gttttgaccg ttcaacattg taaatctgga gaccaggatt
      601 tttcagccca gttgactact attatgaatt tgaatccgga tgtgatttat cttccaaact
      661 attacaccga aattgcactg atttgccgtc aagctcgtga tttaggatat gaagggaaaa
      721 tgttgggtgg cgatggttcc gatgctccgg aattagttga tattggtggc gatgctgttg
      781 aagggtttta tttcaccgct cacggtgata ccgaaaaagc tttaaccgag atcggccaaa
      841 aatacttcga tgcctatcgg gcaaaattta ataaagaacc cggtgttttt ggagctttag
      901 cagctgattg ttatataatc attcttgatg ccatcaaacg agctaactca tttgaaccag
      961 aaaaagtagc cgcagcaatc gaagacacca aagacttaga agtaactact ggaaaaataa
     1021 ccattgaaaa aggcgatgct ttaaaaccgg ttgtagtgaa gcaggttgaa ggtggtaaag
     1081 cgaagcttct tggaaccatc gaagtcaaat aaaaatggga ataaaaaggg acaggtcttt
     1141 cctgtccctt tttatttttt aaaacccttt ttttcttttt gtcttttgag tagaatatat
     1201 cggtatactt tgattcattt tattgaatga tgaggatcta aaaatgagtt ttacccaatt
     1261 ttttcagcaa ctaatgaatg gattttccct tggcagtctc tacgccttat tggcaatcgg
     1321 ttatacgctg gtttacggca tcctcagttt aattaatttt gctcatagcg atgtttttat
     1381 gctcggaact tactttgctt tttatatgat tgccatattt atgcttccct ggtggctttc
     1441 agttataatt agtgtggtat tgtgcgctat tgttggaatg acgattgagc gagtagctta
     1501 tcgtcccctt cggtcagcac ctcgtatttc agccttaatt actgccgtag gagtatctta
     1561 ttttttggaa aatttaggat tagtggtttt aggagccaga ccgaaagggt ttcctcgccc
     1621 tgaccttatg aaaaatgtct atgttattgc cggtgcaaaa attcaagggg tgagtttttg
     1681 ggtaccaatc attagtatcg gtattttaat ggtcttactt tatattgtat ataaaaccaa
     1741 agtaggaatg gccatgcggg ctgtagctaa agatatggaa accacccgac ttatgggtgt
     1801 gaatgttgac cagatcattc tttatacctt cgctctcgga tcggcattag ctgctgctgg
     1861 aggagtcatg tgggctgcca aatttcccca gatcaatccg ttgatgggta ttttcccagg
     1921 gtggaaagca tttaccgct
//
LOCUS       DAOSOL010000276         1922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_59827_length_1922_cov_3.101232, whole genome shotgun sequence.
ACCESSION   DAOSOL010000276 DAOSOL010000000
VERSION     DAOSOL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59827_length_1922_cov_3.101232"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK171_06185"
     CDS             <1..241
                     /locus_tag="PK171_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOT05811.1"
                     /translation="TSIFAFIIAWNEFFFALIFASTQSSKTLSVLVEEFSSKFGSDFI
                     SMSTAGVLASLPPVILALIFQKFIIQGLTAGSVKG"
     gene            897..>1922
                     /locus_tag="PK171_06190"
     CDS             897..>1922
                     /locus_tag="PK171_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05812.1"
                     /translation="MKTQKNVWILLLAMVWILSIVMVSNSQEQIAVIMPRHEMDVKGL
                     WEQQTREFEKETGIKVELITMAWDRVADKVLTELAAGGTSFDVIEFDNAWVAKFNEAG
                     WLVPLNQYMEADVIDQMLPGLVNTFSVDGTLYGIVWNNDTRFFMYNGKKLKDAGIAEP
                     PHTWAEFVEQSQKMITANLIQYGSSEAAEQSQSLANWHTFISYSFGSDLFNNEGKPTF
                     LEQPAIDALTFMADCFLNTKVIDPASLTMNQEMTANVFYNGSTAFFPQAWPGVYNASN
                     DPSISQIVGEIEIAEYIPAVKPEYQATLNLPEALAIPATSKNPEAAWKYIEYMTSPER
                     DKVRSLEI"
BASE COUNT          598 a          341 c          410 g          573 t
ORIGIN      
        1 cacatcgatt tttgctttta ttattgcctg gaatgagttt ttctttgctt taatttttgc
       61 ttcaacccag tcgtcgaaaa ccctgtcggt tttggttgaa gaattcagta gtaaatttgg
      121 ttctgatttt atttccatga gcacagccgg agttttagcc agtcttccac cagttattct
      181 ggctttaatt tttcaaaagt ttatcattca ggggttaaca gctggatcgg tgaaagggta
      241 aatattctta atagtatcgt accaggtagg aaggatagtt tctcctacta cttttatggc
      301 agaaaatggg gtgaaaatcc aaaattcgac atgccatggc atgtcgctac attaaatgaa
      361 gaacgggcac aatacattgt acccctgaat tattaattct tttgtggggc ttgatttatc
      421 atgcccgtgg atatttagga tagactttca tgctgcttta cagcattagt tgatgatgat
      481 aagagttgcc ccctcaccct gttccttttc cacggagggg cgagggaaaa ataagttccc
      541 cctttagcaa aaggagtaag ggggatttga atttttggaa accaatcgaa tcctgaatgt
      601 tctctcttta ggcaaaggga gagaaaaaaa agattgaact catgagattg ccacgtcact
      661 tcgttcctcg caatgacgat tcagagttcg aaggtgagga tgagggtgaa agcacaaaat
      721 gaaatcctgg ttggttgctc taaaagaaaa cctttaaaaa acgttctggt actttcaagg
      781 tagataaatt tttattcact gttataaata ggaagattat aaaaaaataa aagaaggagg
      841 tgtaatcaga ataagattat tttgagtaga ataaaaccaa atttggaggt aaaaatatga
      901 aaacccaaaa aaatgtttgg attttattgt tagcaatggt ttggatttta tctatagtaa
      961 tggttagtaa tagccaggaa caaattgcag tcattatgcc tcgtcatgag atggatgtta
     1021 agggtttgtg ggaacaacaa acccgagaat ttgaaaaaga aaccggtatc aaagtagaat
     1081 taatcaccat ggcctgggac cgagtggcgg ataaagttct caccgaattg gctgcaggtg
     1141 gaacatcttt tgatgttatc gaatttgata atgcctgggt agctaagttt aatgaagccg
     1201 gttggttggt tccgctcaac caatatatgg aagctgatgt tatcgatcaa atgttaccag
     1261 gtttagtgaa tactttcagt gtcgatggca ctctttatgg tattgtatgg aataacgata
     1321 cccgtttttt catgtataac ggaaagaagt taaaagatgc tggtattgct gaacctccac
     1381 atacctgggc agaatttgtc gagcagtcac agaaaatgat taccgccaac ttgattcaat
     1441 acggaagcag tgaagctgct gaacaaagcc aatctcttgc gaattggcat acctttataa
     1501 gctatagctt tggttctgat ctgtttaaca atgaagggaa accaaccttt ttagaacaac
     1561 cagcgatcga tgctctcact ttcatggctg attgcttcct gaataccaag gtcattgatc
     1621 cagcctcgct caccatgaac caggaaatga ccgctaatgt tttttataat ggaagtacag
     1681 cctttttccc ccaagcttgg ccaggtgttt ataatgcctc aaacgaccca tcaatttcgc
     1741 aaatagttgg agaaattgaa atagctgaat atatcccggc agtaaaacca gaatatcaag
     1801 caaccttgaa ccttccagaa gctttggcta ttccagcaac atcaaaaaat cccgaagcag
     1861 cctggaaata tattgaatat atgaccagtc cggagagaga caaagttcgt tctttggaaa
     1921 tc
//
LOCUS       DAOSOL010000277         1920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_59912_length_1920_cov_3.816622, whole genome shotgun sequence.
ACCESSION   DAOSOL010000277 DAOSOL010000000
VERSION     DAOSOL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59912_length_1920_cov_3.816622"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1483)
                     /locus_tag="PK171_06195"
     CDS             complement(206..1483)
                     /locus_tag="PK171_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05813.1"
                     /translation="MRKYLLVCLISLVMLTLSFVAVSQEKEISVLLTGSPLADVMETL
                     QDDFEKETGIKVNVEIVGYEDTHTKGILDLVSGTNNYDLLMVDNPWIAEYVQTGNLLV
                     LDEFMAKEDPEYLAGFDKAVLDAYGLYEGKYYGYPIMLTTTILFYRSDLFEQYGKKAP
                     ETWDEFNEIAKFFTQSFNPESPVKYGISIAGERGDGAISQWLPLLWGYGGSVFDKDWN
                     VTLNTPEALKALEAFVESAQYSAPGFGTNYFPEQVAHFTNGEIAMMENWDAFAGDVAD
                     PVKNPYAENTAYASIPGKYPLLGGWALSINKNSKDLDSAYTFLRWACGPEVTKKSLEV
                     SISTPIRIDVAQDPEVKAIVPWVDVVYEEFKNIRKYAETYQGGQILIPEAEYHVLLGT
                     AINEALTGQLSAKDALAKAQKDIEDLLKKHGYK"
     gene            complement(1563..>1920)
                     /locus_tag="PK171_06200"
     CDS             complement(1563..>1920)
                     /locus_tag="PK171_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05814.1"
                     /translation="YEKGIEAIGYGKPASEVDRTVRSILDPQGLSPLYSLVHGIGWTE
                     AMEGKGAATQHSTWNFPKGIAFMLDLGIFGRSFKSLDKNHVGLRIENPFIIDHEGNTK
                     CLTVDLPLYCETGKIY"
BASE COUNT          571 a          381 c          364 g          604 t
ORIGIN      
        1 aaataccagt agtgaaagct cgtataaaga gaatatagaa aagggtaaat gactataaca
       61 aagagaacga ctaatgccgg taaaagaaga aaatagggag ttaaattgga tttcatttta
      121 atcatatgaa atatttcacc cctttctgct ccttacaaat aaggcatcag gcggggaata
      181 gacttccccg ccttcctttt tatttctatt tatatccgtg tttcttgagg agatcttcaa
      241 tgtctttttg tgctttagct aatgcatctt ttgcagagag ctggccggtt aaagcttcat
      301 tgatagcggt tcctaataaa acatggtatt cagcttctgg tataagaatt tgaccgcctt
      361 gataggtttc ggcatatttt cgaatatttt taaattcttc ataaacaacg tctacccaag
      421 gaacgattgc ttttacttct ggatcctggg cgacatctat ccggatgggg gttgagatag
      481 atacttcgag tgatttttta gttacttcag gcccacatgc ccatcgcaaa aaggtatatg
      541 ctgaatcaag atcttttgag ttcttgttaa tggatagagc ccatcctcct aaaagagggt
      601 atttccctgg gatagatgca taagcagtat tttctgcata gggattcttg actggatcag
      661 caacatctcc ggcaaaggca tcccagtttt ccatcattgc tatctcacca ttggtaaaat
      721 gagcaacttg ttctgggaaa tagttggttc caaagcctgg ggccgagtat tgagcagatt
      781 ctacaaatgc ttcaagtgct tttaatgctt caggtgtgtt cagagtcaca ttccaatctt
      841 tatcaaaaac acttcctcca tatccccaca acaaaggcaa ccattggctt atggctccat
      901 ctcctctttc tccagcaatg gatataccat atttgacagg agattctgga ttaaaggatt
      961 gagtaaagaa tttcgcaatt tcattgaatt catcccaggt ttcgggagct ttctttccgt
     1021 attgttcaaa aagatcgctt cgataaaaga ggatagtagt agttaacatg attgggtaac
     1081 cataatattt tccttcatat aaaccataag catcaagaac tgctttgtca aaaccggcta
     1141 agtattcggg atcttctttt gccataaatt catcgagtac taacaagtta ccggtttgga
     1201 catattcggc aatccaagga ttatcaacca tcagaaggtc atagttattg gtaccggaaa
     1261 caagatctaa aatgccttta gtgtgggtat cctcgtaacc gacaatttca acattaacct
     1321 tgataccagt ttctttttca aaatcatctt gtagggtttc cataacatca gcaaggggtg
     1381 atccagtaag aagaacactg atctcttttt cttgtgatac tgcaacaaat gaaagggtaa
     1441 gcattaccaa tgatattaaa caaacaagta aatactttct catttttttc ctccttttat
     1501 tcaagggcaa aacttaaatt gacttagttt tgccctttat tatccttaat tggtcaaatg
     1561 agttaataaa ttttaccagt ttcacagtaa agcggaaggt cgacggttaa gcatttggta
     1621 ttaccttcat gatcgataat aaatggattt tcaatccgaa gacccacatg gtttttatca
     1681 aggcttttga atgaacggcc aaatattcct aaatcaagca taaaagctat tcctttgggg
     1741 aaattccagg ttgaatgctg ggtagcggct ccttttcctt ccatggcttc ggtccaaccg
     1801 ataccatgaa ctaaactgta taggggggat aacccctggg gatcaagaat cgagcgaacg
     1861 gttcgatcaa cttcgctcgc aggtttacca taaccaattg cctcgatccc tttttcatat
//
LOCUS       DAOSOL010000278         1915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_60106_length_1915_cov_3.805376, whole genome shotgun sequence.
ACCESSION   DAOSOL010000278 DAOSOL010000000
VERSION     DAOSOL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60106_length_1915_cov_3.805376"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /gene="gatB"
                     /locus_tag="PK171_06205"
     CDS             <1..1340
                     /gene="gatB"
                     /locus_tag="PK171_06205"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOT05815.1"
                     /translation="LVCPVCLGLPGSLPVMNHYAMQLAIRTASSLHCQLLPKVVFHRK
                     NYFYPDLPKGYQISQYDLPIGVNGYFEFLTQGEKKQVRIKRVHIEEDAGKLIHEGINL
                     PENSSGVDFNRCGIPLVEIVTEPDLHHSEEARDSLIALRDLVRFIEVSDGNMEEGSLR
                     CDANISISSHPDQMGAKVEVKNMNSFRSVFRALEYEIDRQKRCLEEGNCVPQETRHWD
                     EINEVTVSSRGKEDAEDYRYFPDPDLLPYLVQEEVIQQIESSLPELPLERKERFMIDF
                     ELTEQDANVLVQDKSLADFFEICIQELNKPKFIANWTITEVLKNLNALDIDIKQSKIT
                     PSSFMELLKMIDKKEISGTIAKTILEEMFQTGGRAKEIMAKKGISQINSEKEIEKIVR
                     QVIEQNPQSVADFLGGKEKAFHFLIGQVMKTTRGQANPDLVKSILNKELSAEG"
     gene            1345..>1915
                     /locus_tag="PK171_06210"
     CDS             1345..>1915
                     /locus_tag="PK171_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT05816.1"
                     /translation="MNFTHLHLHTEYSLLDGSIRIQELLQKVRETGMESIAITDHGVM
                     YGVIDFYKQAQNAGIKPIIGCEAYIAPGSRFEKTITKGEENAFHLVLLARNQTGYQNL
                     VKLVSLGFLEGFYYKPRIDRALLQEHSEGLIALSACLAGEIPSLILAGKQKEAENLAA
                     EYRELFGSDSFYLELQENKIPEQTQVNRAL"
BASE COUNT          601 a          375 c          401 g          538 t
ORIGIN      
        1 gcttggtctg tccagtttgt ttggggctgc caggttcatt accagttatg aatcactacg
       61 ccatgcaact agccattcga acggcttctt cacttcactg tcagttgcta ccgaaggtag
      121 tttttcaccg aaaaaactac ttttatcccg atcttcccaa ggggtatcaa atctctcaat
      181 atgaccttcc tattggtgtt aatggttact ttgagttttt aacccagggt gaaaaaaaac
      241 aggttcggat taaacgagtc catattgaag aagatgccgg aaaactcatc catgaaggaa
      301 tcaatcttcc tgaaaacagt tcaggtgtag actttaaccg ttgcggtatc cctctggttg
      361 aaatagtcac cgaacccgac cttcatcact ccgaagaagc ccgggatagt ttaatcgctc
      421 ttcgggattt ggtgcgattc atcgaagtca gcgatgggaa tatggaggaa ggatcattac
      481 gatgtgatgc caatatatcc atttcatccc atcccgatca aatgggagcc aaggtcgagg
      541 ttaaaaatat gaattctttc cgttcggtat tccgggcatt ggaatatgaa attgatcggc
      601 aaaaaagatg tttagaagag ggtaattgtg ttccgcaaga aactcgtcat tgggatgaaa
      661 tcaatgaagt aactgtttct tcccggggga aagaagatgc ggaagattac cgttactttc
      721 ccgatcccga tcttctccct tatctagttc aggaagaagt tattcaacaa atcgaatcat
      781 cacttccgga actcccctta gaacgaaagg aaagatttat gattgatttt gaacttactg
      841 agcaagacgc caatgttctg gttcaagata aatcgttggc cgatttcttt gaaatttgta
      901 tacaggaatt aaataaacca aaatttattg ccaattggac cataactgaa gtcctaaaaa
      961 atctgaatgc tcttgatata gatattaagc aaagcaagat tactccttca tcttttatgg
     1021 aattactcaa gatgattgat aaaaaagaaa tcagcgggac cattgccaaa actatcctgg
     1081 aagagatgtt tcaaaccggg ggtcgagcta aagaaatcat ggcaaagaag ggaatttctc
     1141 aaattaacag tgaaaaagaa attgaaaaaa ttgttcgtca agtcatcgaa caaaatccac
     1201 aaagtgttgc tgatttttta ggtggaaaag aaaaagcctt tcattttctt attggtcaag
     1261 tcatgaaaac cactcgaggg caagctaatc ccgatctagt aaaaagcatc ctcaataaag
     1321 aattatcggc cgagggataa ccaaatgaac tttactcacc ttcatctcca taccgaatac
     1381 agcttattgg atggatcgat tcgcattcaa gaacttctcc aaaaagtacg agaaaccggt
     1441 atggaatcga tagctatcac cgaccatggt gtgatgtatg gagtcatcga tttttataag
     1501 caagctcaga atgccggcat caaaccgata attggatgtg aagcttatat tgcccccggc
     1561 agtcgttttg agaaaactat aactaagggt gaagaaaatg cttttcattt agttttactg
     1621 gctcgcaatc aaaccggata tcagaatctg gttaagctgg tgagtttagg gtttcttgaa
     1681 ggattttatt ataaaccccg tatcgatcgg gctttgttac aagagcactc ggaaggcctc
     1741 atcgctctca gcgcctgttt agccggggaa attccctcgc tcattttagc tgggaagcaa
     1801 aaagaggctg aaaatttagc agctgaatac cgtgaacttt tcggaagtga ttctttttat
     1861 ttagagctgc aagaaaataa aattcccgaa caaactcagg ttaatcgggc tttga
//
LOCUS       DAOSOL010000279         1914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_60158_length_1914_cov_3.144164, whole genome shotgun sequence.
ACCESSION   DAOSOL010000279 DAOSOL010000000
VERSION     DAOSOL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60158_length_1914_cov_3.144164"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PK171_06215"
     CDS             <1..997
                     /locus_tag="PK171_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05817.1"
                     /translation="FQRLFGPKDNPIYRSHFLWNHCSVFIPRLTGQNPVLEMIAQIQS
                     QGSTLDPTAVESLMETLKELYGLKGTLFQQYITTWKRVFALDFGPSFFQFPTPVSELL
                     KTYLPWTIGLFATSTLISWILGNILGGIAGYFSRKNWSKIIDALIMIIRPIPHYIFGI
                     IVLILFAYVWQLFPVGGMMLGRRTVLNWRFIMTILRYSFLPAMTLVILGGAVWFQQMK
                     LLVQNVKREDYVQYAQTGGVKERFIIYRYVLRNAMLPQITQLALALGQVFSGVLIVEM
                     VFSYPGIGLLLYRGVTRGDYNLVMGITVLSILTITTAVLILDLFYPLFDPRIRHK"
     gene            1008..1862
                     /locus_tag="PK171_06220"
     CDS             1008..1862
                     /locus_tag="PK171_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05818.1"
                     /translation="MGVLKDLFKDYRFAVGFILICFLSFLAVASFFSPYDPIEWLQVP
                     RDKAPSWSIILGTNSKGQDVFWEATFAIRNSLIIALIAGLISRVIAVLVGFIAGYKGS
                     IYDRVLMGITDGLLVIPLFLILVLVAMLLREALTLVSTGLLLAFFGWAWDARTIRSQV
                     LSLREREFTLTSVLSGNGTLSLVSKEYLPLTIPLIFSTLINNIAWAIGMEIVLAILGL
                     VSLDIPTLGTTLQWAINYQAMLLGHWWWILTPVILTILLLVALYWFSISVSEYLDPRM
                     RIQRIGAK"
BASE COUNT          488 a          384 c          381 g          661 t
ORIGIN      
        1 ttttcagaga ttatttggtc ccaaggataa tccaatatat cgtagtcatt ttttgtggaa
       61 tcactgcagt gtttttatac cccgcttgac tggtcaaaat ccagttttag aaatgatcgc
      121 tcaaatacag tctcaaggat ccaccttgga tcccacagcg gtagaaagcc ttatggaaac
      181 acttaaggaa ctttatggtt taaaagggac tctctttcaa caatatataa ctacctggaa
      241 aagagttttt gccttagatt tcggtccatc ttttttccag ttccccactc cagtttctga
      301 actccttaaa acctatcttc cttggaccat tggacttttc gctacttcca ctctcatatc
      361 ttggatattg ggaaacatat taggaggtat tgccggatat ttttctcgta aaaactggtc
      421 taaaattata gatgctctca tcatgattat cagaccaatt cctcattata tattcggtat
      481 tattgtcctc atcctttttg cctatgtttg gcagcttttt ccggttggtg ggatgatgtt
      541 aggaaggaga actgttctga actggagatt catcatgacc atactgcgct attcttttct
      601 tcctgcaatg actctggtca ttcttggcgg tgctgtctgg tttcaacaaa tgaaactctt
      661 ggttcaaaat gtaaaaagag aagattatgt tcaatatgct caaaccggag gggtaaaaga
      721 aagatttatt atttatcgtt atgtattacg aaacgccatg ctaccgcaaa ttacccagtt
      781 ggccttagct ttgggacagg tattcagcgg tgttttgatt gttgaaatgg tattttccta
      841 tccaggaatc ggtttgctcc tttaccgggg agtaacccga ggtgattata acctggttat
      901 ggggattacc gttctttcta tactcactat cactacagcg gttctaattt tagatctctt
      961 ctatccattg ttcgatccaa gaatccgaca caaataggtg gtaaaatatg ggagttttaa
     1021 aagacctttt taaagattat cgatttgctg taggttttat ccttatttgt tttttatctt
     1081 ttttagccgt agcctcattt ttttctcctt atgaccccat tgagtggcta caggttccac
     1141 gcgataaagc tccgtcttgg tccattatac tgggaaccaa ttctaagggc caggatgttt
     1201 tttgggaggc aacttttgcc atacgcaatt cgcttataat tgcccttatt gctggactca
     1261 tttcaagagt cattgcagtc ctggtcggat tcatagctgg atacaaaggg agcatttacg
     1321 accgagtgct gatgggaata actgatggac tgttagttat ccctttattt ctgattttag
     1381 tcctggttgc tatgttattg cgcgaagctt taactttagt gagtaccggt ctcctattag
     1441 cttttttcgg atgggcatgg gacgcgagaa ccatccggtc tcaagtttta agccttcgtg
     1501 aacgagaatt taccctaacc tcagttcttt ctggaaatgg cactctctca ttggtaagta
     1561 aagaatatct ccctttaacc attcctctaa ttttctcaac ccttatcaat aatatagcct
     1621 gggctattgg gatggaaatt gttttagcaa tcctcggttt ggtcagcttg gatattccta
     1681 ccttgggtac gacgttacag tgggcaatta actatcaagc catgcttctt ggacactggt
     1741 ggtggatttt aaccccagta attttaacca tcctcctttt agtcgctctc tactggtttt
     1801 caatcagcgt gagtgaatat ttagatccca ggatgaggat tcagagaata ggagcaaaat
     1861 aatgaatgga ctcatcgaag ttgaacaact ccagactttt tatgttttgg aata
//
LOCUS       DAOSOL010000280         1912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_60261_length_1912_cov_2.448573, whole genome shotgun sequence.
ACCESSION   DAOSOL010000280 DAOSOL010000000
VERSION     DAOSOL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60261_length_1912_cov_2.448573"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /gene="fusA"
                     /locus_tag="PK171_06225"
     CDS             <1..1665
                     /gene="fusA"
                     /locus_tag="PK171_06225"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT05819.1"
                     /translation="ASFQATLESIKQKLHAPIVPVVYPIGEEHDFMGVIDLMEMKMII
                     YDKDEEGMEFTVKEVTPEYLEAATAARAAMVEKIAGEDETLMEKFLNDQEISVPELKA
                     VLRRATINRKLFPVYCGASWRNKGIHPILDAIVDYLPSPADIPPTQGFDVVTNEVISR
                     KADNQEPFSGLLFKVQSDPHVGTLSYVRIYSGTVKTGQEVYNSTKQKNERIGRLLLMH
                     ADKREGIEEGYAGEIIACIGLKESTTGDTLCDKLHPISLSPIQFSEPVISLSIEPETT
                     DDQEKMGAALNRLASEDPTFKVSYNHETGQTIIAGMGELYLEIIVDRLKREFGVNVKT
                     GAPQVAYRETISTSAEGEGKYIHQSGGHGQYGHCKVRIEPKNRGEGIEFVNAIKGGVI
                     PANFIPAIEKGVRETLTKGIIAGYPCTDIKVSVYDGSYHEVDSSEAAFKLAGSYAVKD
                     AFLQANPLILEPIMKFEVATPSEFLGAVIGDLSSRRGQVGGTTEAFGFTTVEATIPLE
                     AVRGYATVIRSLTQGRGSFYMEPSHYDPVPRDIQEKLTIKTTDKKE"
BASE COUNT          566 a          365 c          400 g          581 t
ORIGIN      
        1 gcttcctttc aggccactct agaatctata aaacaaaaac ttcatgcccc tattgtgccc
       61 gtagtttatc ctatcggcga agaacatgat tttatggggg ttattgactt aatggagatg
      121 aaaatgatta tttatgacaa agatgaagaa ggtatggaat ttacggttaa agaggtaaca
      181 cctgaatatt tagaagcagc gacagcggcc agagccgcta tggtggaaaa aattgccggc
      241 gaggatgaaa ctttaatgga aaaatttctt aatgatcagg aaatttctgt gccggaatta
      301 aaagcggttt tacgccgcgc cactattaat agaaaattat ttcctgttta ttgcggtgct
      361 tcttggagaa ataaggggat tcaccccatt ttggacgcta ttgtcgacta tcttccttca
      421 cctgcagaca ttcctcccac ccagggtttt gatgttgtta ccaatgaagt tattagtcga
      481 aaagccgata atcaagagcc tttttctggt cttttattta aagttcaatc cgaccctcat
      541 gtagggactc tttcttacgt tcggatttac tctggaactg ttaaaaccgg tcaggaagta
      601 tataattcaa caaagcaaaa aaacgaaaga atcggtcgtt tactcctcat gcatgctgat
      661 aaaagagaag ggattgaaga aggttatgcc ggtgaaatta ttgcctgcat tggtttgaaa
      721 gaatcaacca ccggtgatac tctttgcgac aaattgcatc ctatttcctt atcaccaatt
      781 caattttctg aaccggtaat ttccctctcc attgagccgg aaactaccga tgatcaagaa
      841 aaaatgggtg ccgctctcaa ccgtcttgcc tctgaagatc ctacttttaa ggtttcctac
      901 aatcatgaaa cgggtcaaac tataattgcc ggcatggggg aactttattt agaaattatt
      961 gtggatcgcc ttaaaagaga atttggcgtc aatgtcaaaa ccggtgctcc tcaagttgct
     1021 tatcgggaaa caatttcaac tagtgccgaa ggcgagggta aatatatcca tcagtccgga
     1081 ggtcatggtc aatatggtca ttgtaaagtg agaattgagc ccaaaaaccg gggtgaagga
     1141 atagaatttg ttaatgccat taagggaggg gttattcctg ccaattttat cccggctatc
     1201 gaaaaagggg ttcgtgaaac tttaaccaag ggaattattg ccggatatcc ctgtactgat
     1261 attaaagtat ctgtttacga tggctcttat cacgaagttg attcctccga agctgccttt
     1321 aaattagccg gatcctatgc cgtcaaagat gcttttcttc aagccaatcc cctaattctt
     1381 gaaccaatta tgaagtttga ggttgctact ccctctgagt ttttaggggc tgttatcggt
     1441 gatctttctt cgaggcgggg tcaagttgga ggcactactg aagcttttgg ttttaccact
     1501 gttgaagcca ctattccttt ggaagctgtt cgtggttatg ccacggttat tcgttctctg
     1561 actcagggtc gtggttcttt ttatatggaa cccagtcatt atgatcccgt tccccgggat
     1621 attcaggaaa aattaactat caaaactact gataaaaaag aataaatcag tgttatactt
     1681 gggcagtggg taaagaatca ccttccgaaa gaaaatcatt gggtaatttg gctttaaata
     1741 accttaatct ttgaaacgaa tttttttttg ggaaagatga atttgaattt cgcgaattac
     1801 caccaaatat ttctcttgtc cgtgaatctt ttggagaagt aactatagac aaattccgtc
     1861 ataataaaac tgatgaacaa ttaacttaca tcagaattaa tcgagaatgc aa
//
LOCUS       DAOSOL010000281         1903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_60647_length_1903_cov_3.231061, whole genome shotgun sequence.
ACCESSION   DAOSOL010000281 DAOSOL010000000
VERSION     DAOSOL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60647_length_1903_cov_3.231061"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..273)
                     /locus_tag="PK171_06230"
     CDS             complement(46..273)
                     /locus_tag="PK171_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05820.1"
                     /translation="MKSSSLSEKNHQMVVMIINRCYFYSTHVSLIPINEIATSSNQRT
                     VGLLAMTVFDKYFHRHKLLGSMKVYPENPRA"
     gene            574..753
                     /gene="rpmF"
                     /locus_tag="PK171_06235"
     CDS             574..753
                     /gene="rpmF"
                     /locus_tag="PK171_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT05821.1"
                     /translation="MANLTNKTSRSNRDKRRTHWVVKAPNLIECSHCHAFRLSHRVCP
                     ACGYYNGKQVIKSKE"
     gene            766..1752
                     /gene="plsX"
                     /locus_tag="PK171_06240"
     CDS             766..1752
                     /gene="plsX"
                     /locus_tag="PK171_06240"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOT05822.1"
                     /translation="MKIAVDAWGGDYAPVEILKGIKNAVLDDLELVVIGSKQKLSPLY
                     IELNMEEDRFPIEDTPQVIEMKEHPAEAIRKKPQSTIVRGVQLLAQKKIDAFISAGNS
                     GALMAAAWFGLQRIADIERPAIATLIPNVKSSTVLLDVGANVDCKPKQLLHFGVMGAE
                     YAKAALNIERPRVGLLTIGEEEGKGNELVKSAYQYFKNRCDFLNFEFIGNVEGQDIVD
                     GKADVVVCDGFTGNALLKFGEGLLEFINNMLFLHITDEQLRKRLKEVWKRFDRSEIGG
                     APLLGVAGICMVCHGKSKARDLTSAIFRAKDLVEQKMADRIRDGLSNLNYIQ"
BASE COUNT          595 a          310 c          448 g          550 t
ORIGIN      
        1 attgtgcccc tacaatttta tattctttgg taggggcttg gtttatcatg cccgtggatt
       61 ttcaggatag actttcatgc tgcctagcag tttatgacga tgaaaatatt tatcaaaaac
      121 cgtcattgcg aggaggccaa ccgttctttg gttggacgac gtggcaatct cattaattgg
      181 aatcagtgag acgtgagtcg aataaaagta acagcgattg atgatcataa cgaccatctg
      241 gtgattcttt tcagaaagac ttgaagattt cattcttgat cacttttttg tgttaaaaaa
      301 gaaaaagatg agatcctcac gcgggaaagc accgctcagg atgacgcctg tggcgtcaga
      361 tgagatcctc acgcgggaaa gcaccgctca ggatgacacc tgtggcatca gataagatcc
      421 tcacggcttc tgaatacgag gccacaggat gacggaattt ttaagcatta ggatattttc
      481 aggatagaat agattactaa ttatatgtgg ttgattttaa gataaaacga ttacctgaat
      541 tcaaaagata cacttttaag ggaggatata tacatggcaa atcttaccaa taaaacttca
      601 cgatctaatc gtgataaaag aagaactcat tgggttgtaa aggctccaaa tttgattgaa
      661 tgttcccatt gtcacgcttt tcgtctttcg caccgggttt gtccagcatg tggatattat
      721 aatgggaagc aggttataaa atctaaagaa taaccggaga aaagtatgaa aattgccgtt
      781 gacgcatggg gaggagatta tgccccggta gaaatattga agggtattaa aaatgcggtt
      841 ttagatgatt tggagttagt cgttattggc tcgaagcaga aattatctcc tctctatatc
      901 gaattaaata tggaagaaga ccgctttccg attgaagaca caccccaagt cattgaaatg
      961 aaagagcatc cagctgaggc tattcgaaaa aaacctcaat cgacgattgt tcgtggagta
     1021 cagctcttag ctcagaaaaa aatcgatgct ttcatatcgg ctgggaatag tggagcttta
     1081 atggccgctg cttggtttgg tctgcaaaga attgctgata ttgagcgtcc agcgattgct
     1141 actttgatac cgaatgtaaa atcgagcacg gttttgcttg atgttggagc caatgttgat
     1201 tgtaagccta agcagctcct ccattttggt gttatgggag cagagtatgc caaagcggct
     1261 ttaaatatcg aacgtccccg agtagggctt ttgacaatcg gagaagaaga aggaaaagga
     1321 aacgagctag taaagagtgc ttatcaatat tttaaaaatc gctgtgattt tctaaatttt
     1381 gaatttattg gaaacgttga aggccaggac attgtcgatg ggaaagcaga cgtcgttgtc
     1441 tgtgatggtt ttactggaaa tgccttgtta aaatttggag aaggtcttct tgagtttatt
     1501 aataatatgt tgtttttgca tataaccgat gagcaactcc gaaaaagatt aaaagaagta
     1561 tggaaaagat ttgatcgaag cgaaattggt ggcgctcctt tattgggtgt tgcgggaatt
     1621 tgtatggttt gtcacggaaa atcaaaggct cgcgatctca cgagcgctat attccgagct
     1681 aaagatttag tagaacagaa aatggcagat aggattcgcg acggtctgtc aaatcttaac
     1741 tatattcagt aaaaaggaag ataccaaagt tactcactct ctgggaacgt tgctttaagg
     1801 gaaagttaat gatatataaa agacaaggga tatttatatt taatattgta gctggcctga
     1861 aatgaaggag aaataggatg caaactcgtg taaccgatct att
//
LOCUS       DAOSOL010000282         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_60741_length_1901_cov_3.201517, whole genome shotgun sequence.
ACCESSION   DAOSOL010000282 DAOSOL010000000
VERSION     DAOSOL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60741_length_1901_cov_3.201517"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /gene="araH"
                     /locus_tag="PK171_06245"
     CDS             <1..241
                     /gene="araH"
                     /locus_tag="PK171_06245"
                     /EC_number="7.5.2.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Part of the ABC transporter complex AraFGH involved
                     in the high affinity transport of arabinose; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HOT05823.1"
                     /translation="ANAGEGYELRALAAAIIGGVTFEGGRGTIAGTFLGVILLGIVQN
                     GLNILNVSSYFQTLAVGVIIVVAVVVSNLEKIRNR"
     gene            complement(261..335)
                     /locus_tag="PK171_06250"
     tRNA            complement(261..335)
                     /locus_tag="PK171_06250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(301..303),aa:Val,seq:tac)
     gene            complement(426..1589)
                     /locus_tag="PK171_06255"
     CDS             complement(426..1589)
                     /locus_tag="PK171_06255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT05824.1"
                     /translation="MKNFELYNPTRVFFGKGETDRIGKEIKKYGNKVLLTYGGKSIKQ
                     NGLYDRIIKNLQENGLEVFELGGIQPNPRITSVRQGVEICKKEKIDIILAVGGGSVLD
                     ASKIMGIGVYYNGDPWDFMIKKVKPTRMHPLATIITLAATGSEMNHNAVITNEETEEK
                     LGLVDPTIFPTFSILDPENTLTVPRDQTINGIADILAHVFEQYFHPTKEVPLQDRMAE
                     SIIQTVIETTPRLLKNLHDYDARATIMWCGTLALNHLLEGGVDDDWSTHGIQHELSAI
                     YDIPHGAGLAIIFPQWMKYVLDIIPEKFAQFGERVWNIPRNGKSDRELGLAAIERTKN
                     WFREIGAPISLKEVGIGSEHLEEMASKAVSRGPLGSVKKMNKEDVLAIYKMSL"
     gene            complement(1716..1874)
                     /locus_tag="PK171_06260"
     CDS             complement(1716..1874)
                     /locus_tag="PK171_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOT05825.1"
                     /translation="MERHRCTVCGYIYDSRYGDPDSGIDCGILFKDLPEDWVCPICGA
                     GKDKFVKI"
BASE COUNT          530 a          399 c          374 g          598 t
ORIGIN      
        1 agcgaatgcc ggtgaaggtt atgaattgag agcattagcc gccgctatca tcgggggagt
       61 aacctttgaa ggaggacggg gcaccattgc tggtactttt ttgggtgtta tcttattagg
      121 gattgtccag aatggtttga atattttaaa tgtttcatct tatttccaga ctctggcggt
      181 tggggttatt atcgtcgttg cagtagtcgt gagcaatctt gaaaaaattc gaaatcgata
      241 aattctgaat gagaaaaaca tggtgggcga agcaggattt gaacctgcga ccccttgcgt
      301 gtaaggcaag tgctccgccg ctgagctatt cgcccacgtc acgatggcaa ttataacagg
      361 aaaaataaaa gaaaacaacc aaaaataccc tcggaataat cctccaaggg tattttttta
      421 tgagctcaaa gactcatttt ataaatagct aacacatctt ctttattcat ttttttaaca
      481 ctacccaatg gtcctctgct tactgctttg gaagccatct cttctaagtg ttcactgcca
      541 attccgactt cttttaaaga gattggtgct ccgatttctc ggaaccaatt tttggttcgt
      601 tcgatagccg ctaaaccgag ctcacgatcg gattttccgt tacgaggaat attccacacc
      661 cgttcgccaa attgggcaaa cttctcaggg ataatatcta atacatactt catccattgt
      721 gggaagatta ttgccaaccc ggctccatga ggaatatcat agatggcgct caattcgtgc
      781 tgaataccat gagtagacca atcatcatcg actcctcctt ctaacaaatg attgagagct
      841 aaagtaccac accacatgat ggttgcccga gcatcgtaat catggagatt ttttagaagg
      901 cgaggtgtcg tttcaataac ggtttggata atgctttctg ccattcggtc ttgaagcggt
      961 acttctttag taggatgaaa gtattgctcg aaaacatggg caagaatatc agcaatgcca
     1021 tttatagttt gatcacgagg aaccgtcaag gtgttttccg gatcaagaat ggaaaaggtt
     1081 gggaaaatag taggatcaac aaggcctaat ttttcttcgg tctcttcatt ggtgataact
     1141 gcattgtggt tcatttcgga accggttgct gccagggtta taatagttgc taaaggatgc
     1201 attcgagtcg gtttcacttt ttttatcata aaatcccaag gatcaccgtt ataataaacc
     1261 ccaatgccca ttattttcga agcatcgagg acacttcctc ctcccacggc taaaataata
     1321 tcgatttttt cttttttgca aatttcaaca ccctgacgaa cactggttat tcggggattg
     1381 ggttgaattc cgcccaactc aaaaacttcc agcccattct cttgaagatt tttaatgatc
     1441 cgatcataaa gcccattttg tttgatactt tttcctccat aggttaatag gactttattt
     1501 ccgtatttct tgatttcttt accaatccgg tcggtttctc cttttccaaa aaagactcgt
     1561 gttggattgt aaagttcaaa gttcttcata ataaatatac acctccgaac agattaatat
     1621 tttaaatact catgcataac ggatttcttg tggacagttg gtgggtttta ttccttaaca
     1681 atgacaagcc tgttttgttg tttttttgac cggtattata ttttaacaaa tttatcctta
     1741 cccgctccac aaataggaca aacccagtcc tcgggcaaat ctttaaacaa aatcccacaa
     1801 tcgattccag agtcaggatc tccatagcgg gagtcataaa tataaccgca tactgtgcaa
     1861 cgatgtcgtt caatttcaac tttatcggct tcttccggat g
//
LOCUS       DAOSOL010000283         1896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_60988_length_1896_cov_3.260728, whole genome shotgun sequence.
ACCESSION   DAOSOL010000283 DAOSOL010000000
VERSION     DAOSOL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60988_length_1896_cov_3.260728"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1896
                     /gene="glgB"
                     /locus_tag="PK171_06265"
     CDS             <1..>1896
                     /gene="glgB"
                     /locus_tag="PK171_06265"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOT05826.1"
                     /translation="HADRNDPFQILGIHSAESARGVFVRLFYPDLHSAEVVDPDQSEQ
                     PWIMDRIHSEGFYEVFIPNRSFFRYLLRLTKNNGEIILTRDPYSFLPLLTDLDIYLFN
                     EGTHLAIYEFLGAHFKTIDETPGMLFCVWAPNARRVSVVGDFNGWDGRIHQMRMIGSS
                     GIWEIFIPNVPTETRYKFEIKTQEGHLLLKSDPLAFYAEKTPLTASLTYNLSGYNWRD
                     HDWIEKRKSSSLLATPMSIYEVHLGSWKKGEYSSFLSYRELAHQLVDYVQEMGFTHIE
                     LLPICEHPFNGSWGYQATGYFAPTSRFGEPKDFMYFVDYCHQNGIGVILDWVIAHFPK
                     DGHGLGRFDGTALYEHVDPRKGEHPHWGSYIFNYGRREVRCFLVSNAMYWLKEYHIDG
                     LRVDAVASMLYLDYGRNQGEWLPNQYGGKENLEAIDLIRYFNESIYHRFPGIMTIAEE
                     STAWPGVTLPPYTGGLGFLFKWNMGWMNDFLAYMSKDPIYRKYHHQNLTFPLMYAFSE
                     NFILPLSHDEVVHEKRSMINKMPGDWWQKFANLRLCYATMFGFPGKKLLFMGNEIGQW
                     SEWDHDTGLNWFLLDYDTHRKLQLFVKDLNRIYKQEKSLWEIDNSWKGFEWIDCNDSD
                     NSILSFIR"
BASE COUNT          529 a          379 c          394 g          594 t
ORIGIN      
        1 ccatgccgat agaaatgatc cattccaaat tttaggaata cattctgcgg aaagtgctcg
       61 gggtgttttt gttcgattat tttatcctga tcttcactct gctgaggttg ttgaccccga
      121 tcaatctgag caaccatgga taatggaccg tatccattcg gaaggatttt atgaagtatt
      181 cataccaaat cgaagttttt ttcggtatct cctgagatta accaaaaata atggtgaaat
      241 cattttaacc cgagatccct attcctttct tcctctcctc accgatctcg atatttatct
      301 tttcaacgag ggaacccatt tggccattta tgagtttctc ggagcacatt ttaaaaccat
      361 tgatgaaaca ccgggaatgc ttttttgtgt ttgggctcct aatgctcgac gagttagcgt
      421 agttggagat ttcaatggat gggatggtcg aatccaccaa atgagaatga tagggtcttc
      481 aggaatatgg gaaatattta tcccaaatgt gcccacagaa acacgataca agtttgaaat
      541 taaaacccaa gaaggacatc tcctcttgaa atctgatcct ctggcttttt atgcagaaaa
      601 gactccactt accgcctcac ttacttacaa cctgagcggc tataattggc gtgatcatga
      661 ttggatcgaa aaacggaaat catctagtct tcttgccacc ccgatgagca tttacgaagt
      721 ccatcttgga tcatggaaaa aaggagaata ctcttctttt ctcagttacc gagagttggc
      781 ccatcaactg gtggattacg ttcaggaaat gggttttacc cacatagaac tccttcctat
      841 ttgtgaacac ccctttaacg gatcctgggg ctatcaagct actggttatt tcgctcccac
      901 cagccgtttt ggagaaccaa aggattttat gtattttgtc gactattgcc accaaaatgg
      961 aattggtgtg attctggatt gggttattgc tcactttccg aaggatggtc atggactggg
     1021 acgctttgat ggtaccgcac tctatgaaca tgttgatccc cgaaaaggag aacatcccca
     1081 ttggggaagt tatattttta actatgggcg aagagaggtg cgctgttttt tagtctccaa
     1141 cgccatgtat tggctgaaag aataccacat tgatggccta cgagttgatg ccgtggcatc
     1201 tatgctctat cttgattatg gtcgaaatca aggtgaatgg cttcctaacc aatatggcgg
     1261 caaagaaaac cttgaagcaa ttgatcttat tcgatacttc aatgaaagca tctaccatcg
     1321 atttccagga attatgacta tagccgaaga gtctacagct tggccgggtg taacccttcc
     1381 tccttatact ggtggcttgg gattcttatt caaatggaat atggggtgga tgaatgattt
     1441 tctcgcttac atgagtaaag atcctatata tcgtaaatac catcaccaaa atttaacttt
     1501 ccctcttatg tatgcatttt ctgaaaattt cattttgccc ttgtcacatg atgaggttgt
     1561 ccatgaaaaa cgtagtatga ttaataaaat gcctggtgac tggtggcaaa agtttgctaa
     1621 tcttcgtctt tgttatgcaa ccatgtttgg gttcccgggt aaaaaacttc tcttcatggg
     1681 aaacgaaatt ggccaatgga gtgaatggga tcatgatacc ggtttaaatt ggtttctgct
     1741 cgattatgat acccatcgga aactgcaatt atttgtaaaa gatctcaatc gtatatataa
     1801 acaggaaaaa tcgctctggg aaattgacaa ttcttggaag ggttttgaat ggatcgattg
     1861 caatgattca gataattcaa ttctttcctt tattcg
//
LOCUS       DAOSOL010000284         1887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_61388_length_1887_cov_4.564956, whole genome shotgun sequence.
ACCESSION   DAOSOL010000284 DAOSOL010000000
VERSION     DAOSOL010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61388_length_1887_cov_4.564956"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK171_06270"
     CDS             complement(<1..327)
                     /locus_tag="PK171_06270"
                     /inference="COORDINATES: protein motif:HMM:NF018404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HOT05827.1"
                     /translation="MARYDNEKGEIRCSFCGKPQAEVKKLIAGPGVFICNECIDLCND
                     VIREETKNNKKQPEFNLGKLPTPMEMKKFLDQYVIGQERAKITLSVGVYNHYKRILFS
                     LEENEVE"
     gene            complement(314..952)
                     /gene="clpP"
                     /locus_tag="PK171_06275"
     CDS             complement(314..952)
                     /gene="clpP"
                     /locus_tag="PK171_06275"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF001368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOT05828.1"
                     /translation="MQDLKNNYLVPIVVEQTPRGERSYDIYSRLLIDRIVFLGTEIDD
                     VIANLIIAQLLFLDAQSTEKDINLYINSPGGSVSSTLAIYDTMQYLKSDISTICIGMA
                     ASGGAVILAAGTKGKRMALPNSRVMIHQPLGGAQGQVTDIEIQAREMIKIKEKLNSIL
                     ANHTGQPLDKIKKDTERDYFMSAEEAQLYGLVDQVIIPKKERKGETGGNGTL"
     gene            complement(952..>1887)
                     /locus_tag="PK171_06280"
     CDS             complement(952..>1887)
                     /locus_tag="PK171_06280"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05829.1"
                     /translation="EVRKYKIIIRDSDVEKEIEGLQKSKGIWKEKGDLPVETGDMVKV
                     KVEGREFAVMAGYADDKMLLSHAVIGLKKGEAGKFYSLETEADKPVEEIEFIVTEVMK
                     KEIPELNEEFLTQLNPELKSLDDLRLKTRESLEKWAEDLVQIRMEKEAVALLAKKSEV
                     VVPSVLIDHETKHQIDHFIEHIQKDGMTLEKYMEITNTDFESIEKDFRKQALWQLKKM
                     FVLQEYADKNGISVGKEEMNEEMERIAQRTGKEVEDVKQILEKNNKMDDLMDQKLQQK
                     LILDILQKVKTKELEEPINIDQWKALEDPEEEMVE"
BASE COUNT          473 a          454 c          300 g          660 t
ORIGIN      
        1 ttcaacttca ttttcctcta gggaaaaaag aattctctta taatggttat agactccaac
       61 cgagagggtg atcttggctc tttcctgtcc gatgacatat tgatccaaga attttttcat
      121 ctccatcggt gtcggcaatt ttcccaaatt gaattccggc tgctttttgt tgttcttggt
      181 ttcttcacga atcacatcat tacaaaggtc aatgcattcg ttacaaatga agactccggg
      241 gccggcaatc agttttttta cctccgcctg cggtttcccg caaaaagaac agcggatttc
      301 tcctttttca ttatcataac gtgccattcc cgcccgtttc tccttttctt tctttttttg
      361 gaatgataac ctggtcaacc aagccataaa gttgtgcctc ttctgccgac ataaaatagt
      421 cccgttcagt atcttttttt attttatcta atggttggcc agtatgatta gccaaaatac
      481 tattgagttt ttctttaatt ttaatcatct cacgggcttg aatttcaata tccgtcacct
      541 gaccctgggc tccacctaag ggctggtgta tcatcacccg tgaattgggc aaagccattc
      601 tcttcccttt ggtaccggca gccagaatca ctgctccacc gctggcagcc attccaatac
      661 aaatcgttga aatatccgat ttcagatact gcatcgtatc atatatagcc agtgtcgaag
      721 acactgatcc tcctggacta ttaatgtaaa gattaatatc tttttcagta ctttgagcat
      781 cgagaaagag cagttgggca attataagat tggcgatcac atcgtcgatc tccgtcccaa
      841 gaaaaacgat tcgatcaatt aacaggcggg aatagatatc ataggacctt tctccccgcg
      901 gagtttgctc aacaacgata ggaaccaaat aattattttt taaatcctgc attattccac
      961 catctcctct tctggatctt caagagcttt ccactgatcg atattgatcg gttcttctaa
     1021 ttccttggtc ttcacctttt gcaggatatc taaaatcaat ttttgctgga gcttctggtc
     1081 cattaaatcg tccatcttat tattcttctc taaaatttgt ttgacatcct cgacttcttt
     1141 tcccgttctc tgggcaatcc tttccatctc ttcattcatt tcttcttttc caaccgaaat
     1201 accattttta tcggcatact cctgaagaac aaacattttt ttcaactgcc aaagagcctg
     1261 ttttcggaaa tctttttcaa tgctctcaaa atcggtattg gttatttcca tgtatttttc
     1321 taaggtcatt ccgtcttttt gaatgtgttc aataaaatga tcgatctgat gcttggtttc
     1381 atggtcaatc aaaacactgg gaaccactac ttcagatttt ttagcgagca atgccactgc
     1441 ttccttctcc atacggatct gcaccaaatc ctcggcccat ttctccaaag attccctggt
     1501 tttcaatcgt aaatcatcaa gagatttcag ttcgggattg agttgagtta aaaattcttc
     1561 gttcagctct gggatttctt ttttcataac ttcagtcaca ataaactcga tttcttcgac
     1621 tggtttatcg gcttctgttt caagagaata aaattttcca gcctcacctt ttttcaaccc
     1681 aattactgca tggcttaata acatcttatc atcagcatag ccagccataa ccgcaaattc
     1741 tctgccctca actttaactt taaccatatc acccgtttct accggaaggt cgcctttttc
     1801 tttccagatg cctttcgatt tttggagacc ttcgatctct ttttcaacat cggaatcgcg
     1861 aattatgatc ttatattttc ggacttc
//
LOCUS       DAOSOL010000285         1882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_61638_length_1882_cov_2.889436, whole genome shotgun sequence.
ACCESSION   DAOSOL010000285 DAOSOL010000000
VERSION     DAOSOL010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61638_length_1882_cov_2.889436"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..>1882)
                     /locus_tag="PK171_06285"
     CDS             complement(349..>1882)
                     /locus_tag="PK171_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOT05830.1"
                     /translation="SASAADLEDALVQKTVQVAPNLEPVIPHPDQEKEALDKLTAFEE
                     KTGKKPNILFFIMDDVGWGDLGVYGGGAMAGAPTPNMDRLAREGLQLTSAYAQPSSSP
                     TRATILTGRLPMRHGILRPSGKDETGGLEGEITLPQILSEAGYVTQAVGKWHVGENKG
                     SQPQNVGFDDFYGFLSVSDYYTEWRDPYFNPDVALSPERTQMIRNLAFEKNWVHAKKG
                     GEVEPIAEIDIPTCSILDEKWADYSVEFIKNMANSDQPFFLYHCTRGAHFDNYPNPQF
                     LGKSPAKYPYKDVMIELDYILGRLVNALEETGQLENTIIFITSDNGPEMELWPDSGFS
                     PFRGAKGSTWEGGMRVPGIFYWKDMISPGRISDGLFDLADLFNTSLALSGAKDQMPTD
                     RYIDGIDQTSFLLTDIGQSNRKYIYYWLGSNFSAVRVGEYKYMMTAESDDDRDNYLQG
                     GFSGYIMNYYFGKAFNLYLDPKETHNFFLRKVIYSDIFLGPMKAHLATFQKYPPKDIL
                     GK"
BASE COUNT          525 a          409 c          385 g          563 t
ORIGIN      
        1 gtattgctgg atgtattctt ctaaaatatc gtcagaaatt gctttggtgc ttccgatgag
       61 gtctttttta cttaagtaaa aacaatattc acaacggaga ttacacttgg caccgatggg
      121 cttggccata aggtgaaaag ttcgttttgg ggttggtgat aggatgaatt tatagatttg
      181 attggagttc acatggcctc ccatttattg atagatcttt ataccacttc acagcaacac
      241 ctcgttgctc ttttttataa tcataatctt aaacaataaa ttccattcac aggagggatt
      301 tcataatccc tcctgtgaat ggaatttaaa aagggaaaaa tatccttctc acttacctaa
      361 aatatcttta ggaggatatt tttggaaagt agctaaatga gctttcatcg gtcctaaaaa
      421 tatatcagaa taaatgacct tacgaaggaa aaaattatgg gtttccttcg gatcaagata
      481 gaggttaaat gccttcccaa aataatagtt cattatgtat cctgaaaatc ctccctggag
      541 ataattatcc cgatcgtcat cggattcggc tgtcatcata tatttgtatt caccaactcg
      601 tactgccgaa aagttactac ccaaccaata gtaaatatat ttacgattgg attgcccgat
      661 atcagtgagc aaaaaagaag tttggtcaat accgtcgata taacggtcgg ttggcatctg
      721 atctttagca ccacttaaag caagagaagt gttgaataaa tcggcaaggt caaacaaccc
      781 atcggatatt ctcccgggcg agatcatgtc tttccaataa aatatacccg gcactcgcat
      841 tccaccttcc caggtcgatc ctttcgcacc tctaaagggg ctaaagccgc tatccggcca
      901 gagctccatt tcggggccat tatcggaagt tatgaaaatt atggtatttt ccaattgacc
      961 ggtttcttcc agggcattta ctagtcttcc caggatatag tccagttcaa tcatgacatc
     1021 cttataggga tatttagcgg gagattttcc caagaattga ggattggggt aattgtcgaa
     1081 atgagcgccc cgggtgcagt gatagaggaa aaagggttga tcgctatttg ccatattttt
     1141 aataaattcc accgagtaat cagcccattt ttcgtcgaga atcgaacagg ttgggatatc
     1201 gatttcagca attggttcga cttcaccacc ttttttagcg tgaacccagt ttttctcaaa
     1261 ggctaaattt cttatcattt gagttctttc cgggcttaaa gctacatccg ggttgaaata
     1321 aggatcacgc cattcggtat agtaatccga cacgctcaaa aatccataaa aatcatcaaa
     1381 tcccacattt tgaggctgtg aacctttgtt ttctccaaca tgccacttcc caaccgcctg
     1441 ggttacataa ccagcttcac ttaggatctg aggaagggtg atttctcctt ccaatcctcc
     1501 ggtttcatct ttaccgcttg gccgtaaaat accatgccgc attggaagcc gtccggtgag
     1561 aatggtagct cgagtgggtg aggatgaagg ttgagcataa gcggaagtca attgaagacc
     1621 ttctcgggct aaccgatcca tattgggagt cggtgctcca gccatggcac ctccgccata
     1681 aacccctaaa tctccccatc ccacatcatc cataatgaag aaaaggatgt ttggtttttt
     1741 tccggttttt tcttcaaaag cggttagttt atcaagtgcc tctttttctt ggtcgggatg
     1801 agggatgacc ggttccagat ttggagcgac ttgcaccgtt ttctgaacca atgcatcttc
     1861 aaggtctgct gccgacgccg ac
//
LOCUS       DAOSOL010000286         1880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_61734_length_1880_cov_3.158904, whole genome shotgun sequence.
ACCESSION   DAOSOL010000286 DAOSOL010000000
VERSION     DAOSOL010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61734_length_1880_cov_3.158904"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(679..1485)
                     /locus_tag="PK171_06290"
     CDS             complement(679..1485)
                     /locus_tag="PK171_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOT05831.1"
                     /translation="MKKEKPVFAHSFITFVFVMLVVFFGVLTPQVPAAEKTMVVSGHP
                     EYPPFMWREGDAIAGVGPDIFQLLSQELGIRIETPYLGNWEEVQNKARLGEIDGLVAL
                     YYSEERSQYYEYSIPFAEDPVAIFVLSGHEFPFVNWETLIGKKGVSTLGDIYGEEFDT
                     FAEENLTIERLKTVEECFQKLIDKEAEYFVFALYPGLYAASLYNVNDQILPLSPYVSV
                     VQWYIAISHKSPFVSLMPQIDAILKKLIEDQTIENLVSLHSQKWSKDWQL"
     gene            1683..>1880
                     /locus_tag="PK171_06295"
     CDS             1683..>1880
                     /locus_tag="PK171_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05832.1"
                     /translation="MELTINTRKEIIQKMAPEYQRATKKEKIIDELIHLTGYTQTYFP
                     WLLSHQGRKVDLPGQDVKKFRG"
BASE COUNT          596 a          396 c          358 g          530 t
ORIGIN      
        1 tcgaagactt taccgaattt cttcaatatt taaagctatt tttaactgat aaggaagtta
       61 ggtattaaaa agatataaac cctgatgcaa aatagcggta tataaaaaaa gggatagacc
      121 ctcatgctgc ttagcaacac cagatgagga tgaaaatata tggtcaagaa tcaatttccc
      181 cctttagaaa aagaggttag gaggatttga gttttttccc tgctccgtca ttgcgaggaa
      241 cgaagtgacg tggcaatctc atccacccaa tcagtcgttc tgaggagtcc ggagttttta
      301 ccggacgacg tgagaatctc atctttttca attccacaat tccacagcct ttaaaagatg
      361 agatcctcac gccctcacaa aacaagagct caggatgacg tctttattaa tcgttcaggt
      421 cttttcagga taagttcctt ttaataatct tttatcctca tcaccaaata aagaccagat
      481 tgatcttccc ctgattttgt ggagattaag gaaagaaaat gtaataaatc ggttgaagat
      541 cagcttatct caatgtttaa taaactggga tgggaatcca gcgttttagt ttaaaatgct
      601 tcaaagtaag actcctgaca catgaatact ggataccaaa tctacccaaa tggtatgtat
      661 ccgagtagct gaatattttt agagttgcca atctttgctc catttttgcg aatgaagaga
      721 aacgagattt tctatggttt gatcttctat caacttttta agtatggcat cgatttgagg
      781 cattaatgag acaaagggag acttgtgaga aattgctata taccattgaa cgactgaaac
      841 atagggagat aaggggagaa tttggtcatt tacattatag agagatgccg cgtagagtcc
      901 tggataaaga gcaaaaacga aatattcagc ttctttatca atcaattttt ggaaacactc
      961 ttcgactgtt ttgagcctct caatggtgag gttttcttca gcgaaggtgt caaattcttc
     1021 tccgtaaata tctcccaggg tcgatactcc ttttttacca atgagtgttt cccagttcac
     1081 aaaagggaac tcatgcccgg atagcacgaa gatagctacc ggatcctcgg cgaaagggat
     1141 cgaatactca taatattgcg accgttcttc actataatat agtgccacca ggccgtctat
     1201 ttcacccaaa cgggctttat tctggacctc ttcccaattt cccagatacg gtgtttcaat
     1261 ccgaatccca agctcctggc ttaacaattg aaagatgtcg ggacccactc ccgcaatggc
     1321 atcaccctct cgccacataa agggagggta ctcgggatgc cctgacacaa ccatggtttt
     1381 ctcagctgcc ggtacctggg gagttaaaac tccaaaaaac actaccagca tgacaaatac
     1441 aaaagtaatg aatgaatgag caaaaaccgg cttctctttt ttcatgcctt tcaaaaacct
     1501 cctttttttt atactaaaac tctgcttcct gtacaaaagt atagctttca aacctacttt
     1561 ctcgcaacgt ctcgaattga atcatttaaa atcttgatga actcctatcg ttgcctcacc
     1621 taaaaatctt gatatggatt agtctgcatc gaggtgataa aaagaagact ggaggtgacg
     1681 aaatggagct caccataaat accagaaaag aaattattca aaaaatggca ccggaatatc
     1741 aaagagccac taagaaagaa aagatcatag atgaactcat ccacctcact ggatataccc
     1801 agacgtattt cccctggctt ctttcccacc agggacgaaa agttgatctt cctggacagg
     1861 acgttaaaaa gtttcgagga
//
LOCUS       DAOSOL010000287         1875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_61967_length_1875_cov_3.917033, whole genome shotgun sequence.
ACCESSION   DAOSOL010000287 DAOSOL010000000
VERSION     DAOSOL010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61967_length_1875_cov_3.917033"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PK171_06300"
     CDS             complement(<1..746)
                     /locus_tag="PK171_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05833.1"
                     /translation="MTWHWHLLFFLGWISVGIISSSFPTLNISFLFFPLIPIFWVSVP
                     IFFAGKAFVYSSHHGSSLFSAFINAIIGFSHYPKFLWSRRLTLKLPSNDIQTILKESV
                     NITKVSAPDSLFCPFCNIEIPQALRLVSGENITTTKRLIQCPRCGLRFDCCRYCQNYE
                     VSGGQGWMHENSRGKCKVIKEVQNIDTLCDPSMANRLRDMGWDSLYTGLSIPDNFTPP
                     DRCRQFMLDGEKAKIDHIPGMGKIRILLMKL"
     gene            complement(743..1669)
                     /locus_tag="PK171_06305"
     CDS             complement(743..1669)
                     /locus_tag="PK171_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05834.1"
                     /translation="MEIEKKEKKIAIGDLAKTFVESYKKTNLITNIEKKQFPSRINVI
                     RITEELREILFPGYIGRTSLNWLNVEYYVGGKLDRLFDELQSEIAKAYGEEYSNESLS
                     KAECIQLSCDQTLTFFQKIPQIREMLEDDVQAAFDGDPAAKNTDEIVFSYPGVVAISI
                     YRMAHELLVQGVPLIPRMMTEYAHSLTGIDIHPGAKIGRSFFIDHGTGVVIGETTVIG
                     DQVKIYQGVTLGALSFPKDERGKIIRGTKRHPSLENNATVYAGATILGGETTIGKGSV
                     IGGNVWLTHSIPSNTKVIIEEPRLKFFENKKA"
     gene            complement(1679..>1875)
                     /locus_tag="PK171_06310"
     CDS             complement(1679..>1875)
                     /locus_tag="PK171_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT05835.1"
                     /translation="LYLDGEILTPQERKDFEEHIRICRKCCEKFDFEKNLWNLIKLEC
                     LDTPLPATLVNKVLTVINSF"
BASE COUNT          574 a          374 c          368 g          559 t
ORIGIN      
        1 agcttcatta aaagaatccg aatttttccc attccaggga tatgatcaat tttggctttt
       61 tctccatcaa gcatgaactg tcggcagcga tccggcgggg taaaattatc aggtattgaa
      121 agcccagtat ataagctatc ccatcccata tcacgaagcc gattggccat ggaaggatcg
      181 caaagggtat cgatattttg tacttcttta ataactttac acttgccacg agaattttca
      241 tgcatccagc cttggcctcc gctcacctcg tagttttggc aatagcgaca acaatcaaaa
      301 cgtaatccac agcgtgggca ttgtatcagc ctcttggtgg tggttatatt ttctccagat
      361 accaaacgaa gcgcttgagg gatttcaatg ttacagaagg gacaaaagag cgaatcgggc
      421 gcagaaactt tggtgatatt gactgattct tttagaatag tttgaatatc gttcgatggt
      481 aactttaaag ttaatcttcg agaccaaaga aatttcgggt agtgggaaaa cccaataatg
      541 gcattgatga aggcagaaaa aagagaggat ccatgatgag aagaataaac aaaagctttt
      601 ccagcaaaaa aaatcgggac actaacccaa aaaattggaa tgagtggaaa gaagagaaaa
      661 gaaatattta aagtggggaa agagctactg attatcccaa ccgagatcca gcctagaaaa
      721 aacagcaaat gccaatgcca ggtcatgctt ttttattttc aaaaaatttt agccgaggct
      781 cctcaatgat tactttggta ttggagggaa tggaatgagt gagccataca tttccaccaa
      841 ttacacttcc ttttccaata gttgtttcac caccgaggat ggtggctcct gcatagacag
      901 ttgcattgtt ttctaaactg ggatgtcttt tggtgccgcg gataatttta ccccgctcat
      961 ctttggggaa ggaaagtgct cccaaagtaa ctccttgata aatcttaacc tgatctccaa
     1021 tcacagtagt ttcgccgata actaccccag tgccatggtc gataaaaaaa cttcttccga
     1081 ttttggcacc gggatgaatg tcgatgccag ttaggctatg agcatattcg gtcatcatcc
     1141 ggggaatcag gggtacccct tgtaccaata attcatgagc catcctataa attgagatag
     1201 cgaccacacc tggatagcta aaaacaattt catcagtatt ttttgctgct gggtcaccat
     1261 caaaagccgc ttggacatcg tcctcaagca tttctcgaat ctggggtatt ttttgaaaaa
     1321 aagttagtgt ctgatcacaa gaaagctgaa tacattcggc ttttgaaagg gattcgtttg
     1381 agtattcttc accgtaagct ttagcgattt ctgactgcaa ttcatcgaaa agacgatcca
     1441 actttcctcc aacgtaatac tcaacattta accaatttaa actggttcga ccaatgtaac
     1501 ctgggaaaag aatttcacgt aattcctcag ttatacgaat aacattaatt cgtgaaggga
     1561 attgcttttt ttctatgttg gttattaagt tggttttttt atagctttcg acaaaagttt
     1621 ttgccaaatc accaatggca atcttttttt cttttttctc gatctccata tatactcctc
     1681 aaaagctatt aataacagta agaactttat tcactaaagt tgccggaagg ggtgtatcaa
     1741 gacattctaa ttttatcaga ttccaaaggt tcttttcaaa atcaaacttt tcacagcatt
     1801 ttcggcagat tctaatgtgt tcttcaaaat ctttacgttc ttgaggggtg agaatctctc
     1861 catcaaggta aagat
//
LOCUS       DAOSOL010000288         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_62071_length_1873_cov_3.417492, whole genome shotgun sequence.
ACCESSION   DAOSOL010000288 DAOSOL010000000
VERSION     DAOSOL010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62071_length_1873_cov_3.417492"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PK171_06315"
     CDS             <1..529
                     /locus_tag="PK171_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05836.1"
                     /translation="IEKFGLKEGDRAMVWGLLSQETRGLRTKGVIDAFEEAKLVVDYI
                     EISPEVNADAPLGTPIVSGYLSSHPDCKIIVTDHGGLTATLETYLKGAGKGPDDVIGI
                     GFDLTAATVEAIRNGFCDLILDQQPYLQGYLPILQICLTKKYGFSGLHIDTGSGFIDA
                     GNVEAVAKLAEEGIR"
     gene            533..1360
                     /locus_tag="PK171_06320"
     CDS             533..1360
                     /locus_tag="PK171_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05837.1"
                     /translation="MTTQESLVKLCNIKKSFGHVEVLKGINLEINTNEVVGLLGDNGA
                     GKSTLIKIITGVHQPDSGDLYWKGEKLVNFSVAKARDIGIETVFQERALSEEHSLWRN
                     IFMGREITSRWGFIDVKREKEKTERIMKEYMGFTSSALTTDAVVRTMSGGEKQGVAIA
                     RALHFEADLIILDEPTTGLSLSETQKVLGFVDDIKKKGKSCIFITHNIYHVYPVADRI
                     VVLDRGSVVGQFRKDEISLEELVNRLYLVARTGKLNENGTSTVACDQLQDEMQSKMP"
     gene            1464..>1873
                     /locus_tag="PK171_06325"
     CDS             1464..>1873
                     /locus_tag="PK171_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05838.1"
                     /translation="MRSALLRENKSQISVIIVLFGIFILFTIGSPQTFLSYNIYYSFM
                     STIPFFAIMALSLTLVVISGEMDLSFPSIMGFAGWVFTVIFHRTGSPGIALLLALIFG
                     LGAGLMNGLLIVKLKLPSMITTIGTQFFWAGATAV"
BASE COUNT          529 a          327 c          434 g          583 t
ORIGIN      
        1 cattgaaaag tttggtctta aagaagggga tcgggctatg gtttggggat tgctttccca
       61 ggaaactcgt ggcctgagga ccaaaggagt aatcgatgca tttgaagaag ccaaattagt
      121 tgttgattat atcgagattt ctcccgaagt gaacgctgat gccccgctgg ggactccaat
      181 agtaagcggt tatctctctt ctcaccccga ttgcaaaatt atcgtaactg atcatggtgg
      241 actcaccgct actttagaaa cctatttgaa gggagctggc aagggtcctg atgatgttat
      301 tgggattggg tttgacttga cagcagctac tgttgaagcg atacgaaacg gtttctgtga
      361 tttgatactc gatcagcaac cttatctcca aggatatctg cccatactcc aaatttgttt
      421 aaccaaaaaa tatggttttt ccggacttca tatcgatacc ggttccggat ttattgatgc
      481 aggcaatgtt gaagctgtag cgaagctggc cgaagaaggg attcgataat tagtgactac
      541 tcaagaatct ttggtcaaat tatgtaatat caaaaaatcg tttggccatg ttgaggtcct
      601 gaaggggatt aacctggaga ttaacaccaa tgaagtggtt ggtttattag gtgataatgg
      661 tgccgggaaa tccaccttga tcaagattat caccggagtc catcaaccgg actccggtga
      721 tctctattgg aagggagaaa aactggtcaa ttttagtgtg gcgaaagctc gtgatattgg
      781 tattgaaacc gtttttcagg aacgtgcttt aagtgaagag catagtcttt ggagaaatat
      841 ttttatggga agagaaataa cctcccgctg gggatttatc gatgtaaaaa gagagaaaga
      901 aaaaactgag aggattatga aagaatatat gggttttacc tcttcggcat taactactga
      961 tgcagtggtt agaacaatgt caggtggaga aaagcaggga gtggccatag cacgggctct
     1021 tcactttgaa gctgatctta ttatccttga tgagccaacc accggacttt ctttgtccga
     1081 aactcaaaaa gtacttggtt ttgttgatga cattaaaaag aagggtaaat catgtatttt
     1141 tattacccat aatatctatc atgtctatcc ggttgccgac cgcattgtag tccttgatcg
     1201 aggtagtgtt gttgggcaat ttcgaaagga tgaaatatct ttagaagagc tggttaaccg
     1261 actttatttg gtggctcgaa cggggaaatt aaatgaaaat ggaacttcaa ctgttgcttg
     1321 tgatcaactc caggatgaaa tgcaatcaaa aatgccttaa agttgatctt aaaaaccaga
     1381 ggtgaagatt ctcttaaaat agaattggtt tgggacttga aaaatctaat ggtaccattt
     1441 tttgaggatg tacggaggag attatgagat cggcattact aagagaaaat aaatctcaaa
     1501 tcagcgtaat catcgttttg tttgggattt ttatcctttt taccatagga agcccccaaa
     1561 cttttttatc ctataatatt tattattctt ttatgtcaac catccccttc ttcgccatca
     1621 tggcgttatc tttgactcta gtcgtcatct ctggtgagat ggatctttca tttccttcga
     1681 tcatgggttt tgctggttgg gtatttactg tgattttcca tcgaaccgga agtccgggta
     1741 tagcattgct tttggcttta atttttggat taggcgccgg attgatgaat ggcttattga
     1801 ttgtcaaact caaacttcca tctatgataa ccaccattgg tacccagttt ttttgggcag
     1861 gagcaactgc ggt
//
LOCUS       DAOSOL010000289         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_62110_length_1872_cov_4.244909, whole genome shotgun sequence.
ACCESSION   DAOSOL010000289 DAOSOL010000000
VERSION     DAOSOL010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62110_length_1872_cov_4.244909"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PK171_06330"
     CDS             <1..449
                     /locus_tag="PK171_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HOT05839.1"
                     /translation="LALAMLTDACKKGGAMASSSVGGLSGAFIPVSEDAAMTKAVKTG
                     RLNFEKLEAMTSVCSVGLDMIAIPGDTPEEIISAIIGDEVAIGVINNKTVGIRIIPVV
                     GKKPGDEVVWGGLLGETVVIQVNPTNPKNFIGRGGRIPAPITSLRN"
     gene            510..585
                     /locus_tag="PK171_06335"
     tRNA            510..585
                     /locus_tag="PK171_06335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:542..544,aa:Gln,seq:ttg)
     gene            814..>1872
                     /locus_tag="PK171_06340"
     CDS             814..>1872
                     /locus_tag="PK171_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOT05840.1"
                     /translation="MDYRDWCICVLAAGKGKRMKSSQPKVILPVLGRPLINYLLQLEI
                     PNFCGKRLAVVSPFSYDQIQSVVDPAIQLVIQEEPLGTAHAVQSILPHLDSEIQKILV
                     IYADMPLLEANTIHQFMTFFEKNHSPVAILSTKSEIPDGFGRIKRDFQGIPLKIVEEK
                     DCTDDEKKILEINLGIYAFQKNEIEVVLQSIQKNNAQGEYYLTDVLEVARNLNFKTLV
                     YTVPWDTQFINVNSPDDFSTVIGLFRRKKINQLFESGVKIVDPESVYVDWDVKCEKDV
                     WLYPGTIIEGHSEIKENTKIGPYTHLIQSQVGKDCRIEYSVIEDSILDDEVIIGPFSH
                     IRMNSLIRKNARIGNFVEV"
BASE COUNT          576 a          294 c          420 g          582 t
ORIGIN      
        1 ccctggcgct ggctatgcta accgatgcct gtaaaaaggg aggtgccatg gcttcttcgt
       61 cggtaggagg tctaagcgga gctttcattc cggtaagtga agatgcagcc atgactaaag
      121 cagtaaagac gggaagatta aattttgaga aacttgaagc catgaccagt gtttgttcgg
      181 ttgggctgga tatgattgcc atacctggtg atacacctga agagattatt tctgcgatta
      241 ttggtgatga agtagcaatt ggggttatta ataataaaac ggtggggatt cggatcattc
      301 cagtggttgg gaaaaaaccg ggtgatgaag tggtgtgggg aggattgttg ggagaaacgg
      361 tagttattca agtgaatccc actaacccca aaaattttat tgggaggggt gggagaatcc
      421 cagctccaat aaccagtctt agaaattaga gaaagaaaat tgacttcaaa gatactatta
      481 tatataatta attcgctttt tgtttgagtt ggggcgtggc caagcggtaa ggcaagggac
      541 tttggatccc tgattccgaa ggttcgaatc cttccgcccc agccagttga gaataaaacg
      601 cggctgatac ggtcgcgttt tattttttgt aggactgaat tataatagta tattagaata
      661 cgataaaata ttttggttta ggtagtcaag accacttcaa tggttccatt ttaatatact
      721 caattatata ttagactagc catatgagat gtaattcttt cagaagtata tagtgaaatg
      781 atttttttga gttggtttgt ccaggaggtt tttatggatt atcgtgattg gtgtatttgt
      841 gttttagcag ctggtaaagg gaaacgaatg aaatcttccc agccaaaagt catcttaccg
      901 gttttgggaa ggccattgat taactatctt ctacaactgg agattcccaa tttttgtggg
      961 aagcggttag cagtcgtttc tcctttttct tatgaccaaa tccaatcggt tgttgatcca
     1021 gcgattcagt tagttataca agaagaaccc ttgggaaccg ctcatgctgt acaatctatt
     1081 ttaccccatc tcgattctga gattcagaag atattggtta tatatgccga tatgccactt
     1141 ctggaagcca atactattca tcaattcatg actttttttg agaaaaacca ttctccagtg
     1201 gcgattttaa gcaccaaatc agaaattcct gatggttttg gccgaattaa aagagatttt
     1261 caaggaattc ccctgaaaat tgttgaggaa aaagattgta ctgatgacga gaaaaaaatc
     1321 ttagaaataa atttgggaat ttatgctttt caaaaaaatg aaatagaggt agttcttcaa
     1381 tcaatacaaa aaaataatgc tcagggagaa tattacctta ctgatgttct tgaggtagca
     1441 agaaacctca attttaaaac tttggtttat actgtaccct gggatacaca gtttattaat
     1501 gttaactcac cagatgactt ttcaacggtt atagggttat ttcgtagaaa aaaaataaac
     1561 cagctatttg aatcgggagt taaaatcgtt gatcccgaat cggtgtatgt cgattgggat
     1621 gtcaaatgtg aaaaggatgt ctggttgtat cctggaacaa ttattgaagg ccatagtgaa
     1681 atcaaagaaa acacgaaaat tgggccctat acccatttaa tccaatcaca agttggcaaa
     1741 gattgtcgca ttgaatatag tgttattgag gattctattc tggatgatga ggtaattatt
     1801 ggccctttta gccatattcg aatgaattct ttgataagaa aaaatgctcg gattggaaat
     1861 ttcgttgaag tc
//
LOCUS       DAOSOL010000290         1870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_62194_length_1870_cov_3.104132, whole genome shotgun sequence.
ACCESSION   DAOSOL010000290 DAOSOL010000000
VERSION     DAOSOL010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62194_length_1870_cov_3.104132"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PK171_06345"
     CDS             <1..1044
                     /locus_tag="PK171_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT05841.1"
                     /translation="SCLIGCWKESLYIKREMRCAVNPAIGDERFLDLKPALTKLKVAV
                     VGGGIAGMEAARIATLRGHQVTLFEKDDELGGILRTCCMVPPKSKMKWYMDWIREQIK
                     NLKVEVRLNTIAKTDELKMFDVVLCGTGAKTVFPDIPGVEKAIKFEDVLRCAKKNCEY
                     WPKERTVEAVKIGQKVLVWGNHYAATDTAEALALRGKEVIYVTEDKEIAPNIEPIHRE
                     VMMLRFNGGNGQGLEEKAFKFPVNIRLNTTVVEIKDGEVVLMNDAFEKETIAVDTVVL
                     AKTKPNTELYDSLLAAGLHVVNIGDSNLVRNVRGAMTDGANAGLMIDGDIFMNANNAL
                     SCGLPYDVKLQMK"
     gene            1316..>1870
                     /locus_tag="PK171_06350"
     CDS             1316..>1870
                     /locus_tag="PK171_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOT05842.1"
                     /translation="MFKLGFVSAILADLSFEEVVDFASVNGYACVEMLCWPKGKAERR
                     YAGVTHIDVNELDEQKIKSIKKYAGEMNIEISALGYYPNPLDPDPEKRKYYIEHIKKV
                     ITAATKLGLKNVNTFIGKDKNKSIEDNFKTFKKVWPAIIKHAEASNIKIGIENCPMFF
                     TNDEWPGGNNLATTPAIWRRMFEEI"
BASE COUNT          624 a          278 c          431 g          537 t
ORIGIN      
        1 tcctgtttga ttggatgttg gaaagagtca ctctatatta agagggaaat gcgttgcgca
       61 gtaaatccag ccattggtga tgaacgattc ttagatttaa aaccagcatt aaccaagctg
      121 aaggttgccg tggttggtgg tggaatcgct ggcatggagg cagccagaat tgctactttg
      181 agaggacatc aggttactct atttgaaaaa gatgacgaat tgggaggaat tttaagaact
      241 tgttgcatgg ttcctccaaa atcgaaaatg aaatggtata tggattggat tcgagagcaa
      301 attaaaaact taaaggttga agtgaggctc aataccatag caaaaacaga tgagcttaag
      361 atgtttgatg ttgttttatg cgggaccggt gccaagacag tgtttcctga tatccccgga
      421 gtggaaaaag caattaaatt tgaagatgtg cttcgctgtg ccaaaaagaa ctgtgaatat
      481 tggccaaaag aaagaactgt cgaagcggtc aagattggcc agaaagtttt agtctggggg
      541 aatcattatg ctgccaccga taccgccgaa gccttggctt tgagaggcaa agaagtcatt
      601 tatgtaactg aagataaaga aattgctcct aatatcgaac caatccatcg tgaagttatg
      661 atgctgcgat ttaatggtgg aaacggtcaa ggtttggaag aaaaagcctt caaatttcca
      721 gtcaatattc gtctcaatac caccgttgtt gaaatcaaag atggtgaagt agtcctgatg
      781 aatgatgctt ttgaaaaaga aaccattgct gtagatacag ttgtattagc caaaactaaa
      841 cctaacaccg agctatatga cagtttgtta gcagctggcc ttcatgttgt caatatcgga
      901 gattcgaatc tggttagaaa tgttcgagga gcaatgactg atggagcgaa tgctggtttg
      961 atgattgatg gagatatatt tatgaatgcc aacaatgctc tttcctgtgg gctaccttat
     1021 gatgtaaaac ttcaaatgaa ataagaataa caagataggt gagaatgcta agtctttgtt
     1081 gtaatgaaaa ggaaaatagt aataaatatt tttccttttc attgagctga ttttttttaa
     1141 taatagaaag gggatttgaa atccttggga gataatcgaa tccccttttc ttggagtatt
     1201 tcactcagga agagagcttt tttcttaaaa aaaagagagc cactgctgaa ttaaaactga
     1261 ataggtattc aattctcatc atcgatcctg tcattcatga ggaggttcgt tttgtatgtt
     1321 taaacttggt tttgtgagtg cgattttagc tgatttaagt tttgaagaag tggtagattt
     1381 tgcgtccgta aatggttatg cctgtgttga aatgctttgt tggccaaaag gaaaagcaga
     1441 aagaagatat gctggtgtta cccatattga tgttaacgag ttggatgaac aaaagatcaa
     1501 gtcaataaaa aaatacgctg gagagatgaa tattgaaata tcggcgttgg gctattaccc
     1561 caatcctctt gatcctgatc cggaaaaaag gaaatattac attgagcaca tcaagaaagt
     1621 cattacggca gcaacaaaat tgggactaaa aaatgtcaat acctttattg gtaaagataa
     1681 gaataagagt attgaagata attttaaaac cttcaaaaaa gtatggccgg ctattattaa
     1741 acacgctgaa gcaagtaata taaagatagg gatcgaaaat tgccccatgt tttttaccaa
     1801 cgacgagtgg ccaggaggga ataacttagc aactactcca gcgatttgga ggagaatgtt
     1861 tgaagaaata
//
LOCUS       DAOSOL010000291         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_62373_length_1866_cov_3.810602, whole genome shotgun sequence.
ACCESSION   DAOSOL010000291 DAOSOL010000000
VERSION     DAOSOL010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62373_length_1866_cov_3.810602"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PK171_06355"
     CDS             <1..480
                     /locus_tag="PK171_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05843.1"
                     /translation="SFGLSIFTVIFIAVALLTWFILTYTQLGRGIYAIGGDMEAAKRS
                     GMNVFNIQFFIYCYVGILAGIAGVLHVSLIRYSNPTYLVGTELSVIAPVVLGGTRITG
                     GSGTVIGTILGVVMITLLNNSLILIGLSSFWQEFFTGIIVAISVSLSSYQIRKNMMI"
     gene            517..1521
                     /locus_tag="PK171_06360"
     CDS             517..1521
                     /locus_tag="PK171_06360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05844.1"
                     /translation="MFNQYLKHSRTTILFVMMLGAFIIMSIFLPGKFLTLSNFQSIAS
                     QIPEFGLLAIAIMLAMLTGGIDLSIISTANLAGVGAALMLTKYSLPQFGVYEESYLIF
                     LAIVSAISISLLCGLINGLLIAYIKVPAILATLGTMSIFSGIAIVITKGYGIQGFPEK
                     FLFIGSGTILAIPMPMVIFIFCILLMSLILNRTSFGFNLYMLGSNPVAARFSGINNRG
                     ILVRTYILSGLCSGLASIIMISRVNSIRPGYGSAYLLQAILVCVLGGVDPSGGYGNIS
                     GLVMGIIVLQILQSGFNILSFSPFFKNVVWGLMLILVMILNFYSSRYTQKIRKRLKVD
                     "
     gene            1594..>1866
                     /locus_tag="PK171_06365"
     CDS             1594..>1866
                     /locus_tag="PK171_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05845.1"
                     /translation="MAAGKKLKLSVGIWCMGGMCDRFLPQGYEEPLNIQERLKRVKKV
                     PEVEAIEFFDDEFEKISPRDFKSLLEDNGLKVSCINLNTHSRPKWQL"
BASE COUNT          497 a          323 c          402 g          644 t
ORIGIN      
        1 agttttggtt tatcaatatt taccgtgatt ttcatagctg ttgctcttct cacctggttt
       61 atccttacct acactcagtt aggaagagga atatatgcca taggcggcga catggaggca
      121 gctaaacgat ccggtatgaa tgttttcaac attcaatttt ttatttactg ttatgttgga
      181 atactagccg gaatagccgg tgttcttcat gtttcgctga ttcgctatag caatccgact
      241 tatttagtag gtaccgagct gagtgttata gcgccggtgg tattgggtgg aactaggata
      301 actgggggat cgggtacggt cattggcacc atattaggag tagttatgat aacgttgttg
      361 aataatagtc ttattttgat cggtctctct tctttctggc aggaattttt cactggaatt
      421 attgtagcca taagcgtcag cttgagttct tatcagatcc gaaagaacat gatgatttga
      481 tgattctcat taaaaattta ggttggaggg caaatcttgt ttaatcaata tttgaagcat
      541 tccagaacca cgattttatt tgtcatgatg ctgggcgctt ttattattat gtcaattttt
      601 cttcccggta aatttttaac tttaagtaat ttccagtcca tagccagcca aataccggag
      661 tttgggttac tagccattgc catcatgttg gctatgctga ccggtggtat cgatttatca
      721 attatatcaa ccgctaatct tgccggtgtg ggagcagcac tgatgctaac caagtattcg
      781 cttccccaat ttggagtcta tgaagagtct tatctaattt ttttagcaat agtctcagct
      841 atcagcatat ccttgctttg tggtttaatc aatggactac tcattgccta catcaaagta
      901 ccggcaatac ttgccacctt aggtactatg agtatttttt ctggaattgc catcgttata
      961 accaaaggat atggcattca gggattcccg gaaaaatttc tatttattgg aagtggcacg
     1021 atccttgcca ttcccatgcc gatggttatt tttatttttt gcattctatt gatgtcgtta
     1081 atcctgaacc gaacctcttt tggttttaat ctctatatgc tgggttctaa tccggttgca
     1141 gctcgcttta gcggaataaa taatcgagga attttagttc gtacctatat tttaagtggt
     1201 ctttgttctg gtttggcatc gatcatcatg atttcgaggg ttaattcgat ccggccgggt
     1261 tatgggtcag cctatctcct tcaagctatt ttagtgtgtg ttttaggagg ggtcgatccc
     1321 agcggaggat atggaaacat atctggcttg gttatgggaa taattgtttt acaaatcctt
     1381 caaagtggtt ttaatatttt atcttttagc cctttcttta aaaacgtggt ttggggttta
     1441 atgttgattt tggtgatgat cctcaacttt tatagttcaa gatataccca gaaaataaga
     1501 aaaagattga aagtcgacta aatttatata agcagttttt gaataacttt tttcctaaac
     1561 gaaaagctca atgagctgag aaaggagtta caaatggctg ccgggaaaaa actgaaactt
     1621 tctgtaggaa tttggtgcat gggagggatg tgtgaccggt ttttgcctca gggatatgaa
     1681 gaaccgctta acatccagga acgattaaaa cgggtgaaaa aggtaccaga agttgaggcg
     1741 attgaatttt ttgatgatga atttgaaaag ataagccccc gagattttaa atccttactt
     1801 gaagataatg gtttaaaagt gagctgtatc aatctcaaca ctcactctcg tcccaaatgg
     1861 cagttg
//
LOCUS       DAOSOL010000292         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_62813_length_1856_cov_3.162687, whole genome shotgun sequence.
ACCESSION   DAOSOL010000292 DAOSOL010000000
VERSION     DAOSOL010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62813_length_1856_cov_3.162687"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK171_06370"
     CDS             <1..470
                     /locus_tag="PK171_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05846.1"
                     /translation="INDSYINMNILHKMNTMGYSIVTSDMIDEKEIRKALKKIPKPSF
                     WTISNEVLGSALYSIDQKDPVIKGFVHLVSFECGPDSLVGELIERWLKREVDSIPYLR
                     LEIDEHTGEAGLITRLEAFVDMMKWRTKTFESNLSSSCSSECGSGGSLSSTGN"
     gene            397..1497
                     /locus_tag="PK171_06375"
     CDS             397..1497
                     /locus_tag="PK171_06375"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOT05847.1"
                     /translation="MKATFPHLAHLNVVLEVLYPALGIEVIPPPPITKKTIEIGVKLA
                     PQNACFPMKVTLGNFIEGIEKGADTIFMAGGVGPCRFGYYGQIQRIIIEDLGYDVNFV
                     LIDSPRYGWKNFFSSLKTMVGGKWSISRIHRAVRLAWNVLRYVEDLEKTSRLVRWKLK
                     EPSQLDGLMEKSLKEIKNITSVAQLEEIRSNALHEISQLNTIEKNEPIKIGIVGEVYI
                     AQEFRVNFNIEKLLGNMGILVDNSVSTAEYVLNFFPLTRSREKEEAWKLAKPFLKRFV
                     GGEGIDTVGSTIRYAQQGFDGVIHLYPFTCMPEAVAKSVVAGVGREYNIPILHLAIDE
                     HAGEAGMITRVEAFVDVLEMRHQQDVRETSRF"
     gene            1559..>1856
                     /locus_tag="PK171_06380"
     CDS             1559..>1856
                     /locus_tag="PK171_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05848.1"
                     /translation="MDLFLGVDVGSVTTKFALITSDKKIYSSCYLKTSGDPITALKTG
                     LKYIQSHYPSGGEIRGVGTTGSARHLAGVVVGADIVKNEITAHAVAAITLLPEVR"
BASE COUNT          584 a          288 c          413 g          571 t
ORIGIN      
        1 tcatcaatga tagttatatt aatatgaaca tccttcataa aatgaatact atgggttatt
       61 caattgttac cagtgatatg atagatgaaa aggagatacg aaaagcttta aagaaaattc
      121 ctaaacccag cttttggacg atttccaatg aagttttagg cagtgcactc tattcaatag
      181 accaaaaaga tcctgttata aaagggttcg tccatttggt atcttttgaa tgtggtcccg
      241 attcattagt tggtgaactc atcgagcggt ggctaaaaag agaagtggat tcgattccct
      301 atttacgcct tgagattgat gaacatactg gcgaggccgg tttgataact cgtttagaag
      361 cttttgtcga tatgatgaaa tggaggacaa aaacatttga aagcaacctt tcctcatctt
      421 gctcatctga atgtggttct ggaggttctt tatccagcac tgggaattga agttattcct
      481 ccacctccaa ttactaaaaa aactattgaa ataggagtaa aactcgctcc tcaaaatgcc
      541 tgttttccaa tgaaagtgac cttgggcaat ttcattgaag gcatagaaaa gggagctgat
      601 actattttta tggctggcgg agtcggtccg tgccgttttg gatattatgg acaaattcaa
      661 agaataatta ttgaagatct gggctatgat gtgaattttg tgcttattga ttcgcctcgg
      721 tatggttgga agaacttttt ttcctcctta aaaacaatgg ttggaggcaa atggtcgatt
      781 tcacgtattc atcgagcggt tcgattagct tggaacgttc ttcgatatgt tgaggatttg
      841 gaaaaaacca gccgactggt tcgatggaaa ttaaaagaac cttcacaact tgatggattg
      901 atggaaaaaa gcttaaaaga gattaaaaac atcacttccg ttgctcaatt agaagaaata
      961 cggagcaatg ccttgcatga aatatctcaa cttaatacta tagaaaaaaa tgaacctatt
     1021 aagataggta ttgtgggtga agtttatatc gcacaagaat ttagggtgaa ttttaacatt
     1081 gaaaagcttt taggaaatat gggtatctta gtggacaatt cagtttcaac cgctgaatat
     1141 gttcttaatt tttttccttt aacccgttca agagagaaag aagaagcttg gaaattagct
     1201 aaaccttttc ttaaaagatt tgtaggtgga gaagggattg acacggttgg aagtactatc
     1261 cgatatgccc aacaaggttt tgatggagtt attcatttat atccttttac ttgcatgcct
     1321 gaggcggttg cgaaaagtgt tgttgcaggg gtaggaaggg aatacaatat tcctatactt
     1381 catttagcta ttgatgaaca tgccggagag gcaggaatga taacccgtgt tgaagcgttt
     1441 gttgatgttt tagaaatgcg tcaccaacaa gatgttcgag aaacatctcg gttctaaata
     1501 ggaagaattg tttttttatg aatggttaaa aagagggata gttggaggtt agatataaat
     1561 ggatcttttt ctcggggttg atgttggttc agtgacaaca aagtttgcct taattacatc
     1621 agataaaaag atttactcga gttgttattt aaaaaccagc ggtgacccaa ttacagcttt
     1681 aaaaaccggt cttaagtata tccaatctca ctacccatct ggtggtgaaa taaggggggt
     1741 tggaacgaca ggaagtgcgc gccatttagc aggagtcgtg gttggagctg atatagtaaa
     1801 aaatgaaatt accgctcatg cagtagctgc cattactctt ttaccggaag taagaa
//
LOCUS       DAOSOL010000293         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_62832_length_1856_cov_2.519711, whole genome shotgun sequence.
ACCESSION   DAOSOL010000293 DAOSOL010000000
VERSION     DAOSOL010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62832_length_1856_cov_2.519711"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="PK171_06385"
     CDS             complement(<1..1313)
                     /locus_tag="PK171_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOT05849.1"
                     /translation="MARQQPDQETFMSLSQEGYDSIPIYREKLLDRLTPISLFESFRD
                     CKPVILLESAERGEVWGRFSFLIIDPEEEIRISFKDNLIGSLEEKYPQQCIYPQPEIP
                     FLGGAVGFLSYDAVKTWEKTVPQKPLDYPYAYFLTVHRFLYIDHLRHTVGAVQVVPAG
                     KRELYLQAKTWMDDIFNSLEKKGDNVNTSFQLKGVIQSNFSQNGFLGAVEQVKKLIED
                     GHVSQTVVSQKFSAPFKGDPFLAYRCLRSLNPSPYMFYLDAGDFQIAGSSPEMLVKLD
                     KGKLTTKPIAGTRKRSQLRPEAEIVQELLNDEKENAEHIMLLDLGRNDIGKICCPGTV
                     QVEEFMNVERYSHVYHIVSQVSGRMKENCSPWKALQACFPAGTVSGAPKVRAMQIIES
                     LEPCARGPYAGALGYVGDNGNMDTCIIIRSLFFKEGVASVQTGAG"
     misc_binding    complement(1349..1602)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          546 a          417 c          331 g          562 t
ORIGIN      
        1 ataccagctc cggtttgaac acttgctaca ccttctttaa aaaagagact tcgaattata
       61 atacaagtat ccatatttcc attgtctccc acatacccca aagccccagc ataaggacca
      121 cgtgcgcatg gctctaaaga ttcaataatt tgcatagctc tgactttggg tgcaccactc
      181 actgttccag ccggaaaaca agcttgcaga gccttccagg gagaacagtt ttctttcatt
      241 cgaccagaaa cctgggatac tatgtgatag acatgagaat acctttccac gttcatgaat
      301 tcttcaactt gaacggtccc cggacagcaa attttcccaa tatcattccg accaaggtca
      361 agcagcatga tatgttcagc gttttctttt tcatcattga gaagttcttg aacaatttct
      421 gcttcgggcc gcaattgtga acgttttctg gttccggcaa tcggtttggt ggttaatttt
      481 cccttatcaa gcttgactaa catctcggga gatgatccag caatttgaaa atcaccagca
      541 tcaagataaa acatataagg tgaaggattt aacgatctaa gacaacgata agccaaaaag
      601 gggtcacctt taaaaggggc agagaatttt tgtgaaacta ctgtttgtga aacatgccca
      661 tcctcaatca attttttgac ctgttcaacg gctccaagaa aaccattctg agaaaaattc
      721 gattggatta cacctttgag ttggaaagag gtatttacat tgtctccctt tttctctaat
      781 gaattaaata tatcatccat ccaggttttg gcttgaagat ataactctct cttcccagca
      841 ggaaccacct gaactgctcc tacagtatgg cgaagatgat cgatatacag gaagcgatgg
      901 actgtaagaa aataggcata gggataatca agaggtttct gtggaacggt cttttcccaa
      961 gttttaaccg catcgtaact taaaaaacca accgccccac caaggaaggg aatctctggt
     1021 tgaggataaa tacattgttg cggatatttc tcttccaaag agcctattaa attatcttta
     1081 aagcttattc gaatttcctc ttcaggatcg atgatgagaa aagagaacct tccccatacc
     1141 tctcctcttt ctgcactttc caatagaatt actggtttgc aatctcgaaa tgattcaaat
     1201 aatgaaatag gtgtcaatcg atcaagaagt ttttcacgat agattggaat tgagtcatac
     1261 ccctcttggg ataagctcat aaaggtttct tggtccggtt gttgtctcgc cataaaagaa
     1321 tacctccaat aaaaaagcct tcatctctta aagagacgaa ggctttatcc gcggtgccac
     1381 tctttttaac ggtacacaaa taccgttctc tcatccaggt acaggattcc tccgataccc
     1441 tgcttcacat accggcgaag taccgtttcc acctacttgc ttaagctttc gaggaactgc
     1501 tccgaggccc attcattttc ttcatgccac caggctccca cctcgttccc ggctctctct
     1561 ggacatgatc gaaaaactac tcttcctcat catcgcatta tttttttaat ttgaagtatc
     1621 aaaacatatg aattttattt tgtcaatact taaaatagaa attttataaa tcttgataca
     1681 ttctaatcaa tatttcaata aaaaccagtg tctattctgt aaatatagtg agaagtgagt
     1741 tgtgagcagt gaattgaaag aatttcgccc ttacccttac cttctcccat ttttcctttt
     1801 ttcctctccc ctggtgggag aggattaagg tgagggagaa acttggatat ttcatt
//
LOCUS       DAOSOL010000294         1854 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_62919_length_1854_cov_4.126181, whole genome shotgun sequence.
ACCESSION   DAOSOL010000294 DAOSOL010000000
VERSION     DAOSOL010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62919_length_1854_cov_4.126181"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PK171_06390"
     CDS             complement(<1..1364)
                     /locus_tag="PK171_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT05850.1"
                     /translation="MEIQGQVERITFHNEENGYTIARMKVSGNRELITIVGNLLSITP
                     GEILKLQGEWKNHPKYGEQFLIQSYESVVPASVKGVEKYLGSGLIKGIGPVMAKRLVN
                     KFGIETLEIIEKDIQKLQEVEGIGTKRIAMIKKAWDEQKDIRNVMIFLQEHGVSPSYA
                     VKIYKQYGQNSIITVQENPYRLATDIFGIGFITADRIAQKLGHPKDAPARIEAGILYT
                     LNKFAENGHVYVPTSALLSKCHEILDVEENEAKKALSNLSGQDSVFIEEISDHEEGVY
                     LTPFHIAETGIVDRLKALIEFPKTIPLFGLGKTHQIVSKELGINLDESQQQALHAALS
                     EKVLVITGGPGTGKTTLIKAIICLYKKLGYQILLAAPTGRAAKRMTEATGFEAKTLHR
                     LLEYSPKDNQFKKNHESPLNADVLIIDETSMIDTILMYHFLKAIPPNATLILVGDVDQ
                     LPSVG"
     gene            1621..>1854
                     /locus_tag="PK171_06395"
     CDS             1621..>1854
                     /locus_tag="PK171_06395"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT05851.1"
                     /translation="MKEKLVFPDFHLKGYVPETQREEDFSLNSFGGKWVIFYFYPRDA
                     TPGCTKEAIAFTEKLEEIRSKGVEVVGVSTQSVE"
BASE COUNT          555 a          332 c          342 g          625 t
ORIGIN      
        1 cctactgaag gaagttggtc aacatctcca actaaaatga gagtggcatt tggagggata
       61 gctttgagaa aatgatacat taaaattgtg tcgatcatcg aggtttcatc aataataagt
      121 acatcagcat tcaaaggact ttcatgattt tttttaaact ggttatcttt gggtgaatac
      181 tccaatagcc gatgaagagt tttagcttca aatccagttg cttcagtcat tcgcttggct
      241 gctcgaccgg taggcgcagc taaaagtatt tgataaccca attttttata caggcaaatt
      301 atggctttaa tcagagtggt ttttccagta cccggtcctc cagttatgac caatactttt
      361 tcagatagtg cagcgtgtaa agcctgctgt tgactttcat ctaagttaat acctaattct
      421 ttagatacaa tttggtgggt tttccccaac ccaaacaacg gaatagtttt gggaaactca
      481 atgagtgctt ttaacctgtc tacgattccg gtttcagcaa tatggaatgg agtaagatag
      541 actccctctt catgatcgga tatttcttca ataaataccg aatcttggcc agataagttt
      601 gaaagggctt ttttggcttc attttcctcg acatcaagga tttcatgaca ttttgataag
      661 agagccgagg ttggaacgta aacatgacca ttttcagcaa atttgttaag agtgtaaagt
      721 ataccagctt caattcgtgc cggagcgtcc ttagggtgac ctaatttttg tgcaattcga
      781 tcagcagtga taaagccgat tccgaaaata tcagttgcta atcgataggg attctcttga
      841 actgttataa tcgagttctg tccatattgt ttatatattt ttactgcata agaaggactt
      901 actccatgtt cttggagaaa aatcatcacg tttcggatat ccttttgctc atcccaagct
      961 tttttgatca ttgctattcg cttggttcca atgccttcaa cttcttgcaa tttttgaata
     1021 tccttttcga tgatttccaa tgtttcgatt ccaaacttat taaccagcct cttcgccatg
     1081 actggaccaa tgcccttaat gagaccggaa cctaaatatt tttctacacc ttttactgat
     1141 gccgggacaa ccgattcata agattgaatc aggaattgtt cgccatattt tggatgattt
     1201 ttccattctc cttgaagctt taaaatctct ccaggagtta tggataataa attaccaaca
     1261 atagtaatga gttcccggtt tccagaaact ttcattcggg ctatggtata gccattttct
     1321 tcgttatgaa atgttattct ttcaacttgt ccttgaattt ccatgtagct tttacctcgg
     1381 atattcactt gatttcatta taaaccatta gaaaatgtag atttgtttat attgatcatt
     1441 ataattgaaa cttttaaaat caaagactca agaatcaagg ctccctttta tgccttttaa
     1501 tcccagcaaa actcagattt tattaagtaa tagggaagaa tattggaggt taatggttat
     1561 ctgttaaaga gcaaaaaaaa gattgaaaaa tattacttag aataattgga ggttaaaaac
     1621 atgaaagaaa aacttgtatt tcctgatttt cacctcaaag gttatgttcc agaaacccaa
     1681 agagaagaag atttttcttt gaatagcttt ggtggaaaat gggtaatatt ttacttttat
     1741 cctcgagatg caactccagg atgtaccaag gaagcaattg cttttactga aaaattagag
     1801 gaaattcgtt ccaaaggagt agaagtagtt ggtgtgagca ctcaatcggt ggag
//
LOCUS       DAOSOL010000295         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_63312_length_1846_cov_3.838079, whole genome shotgun sequence.
ACCESSION   DAOSOL010000295 DAOSOL010000000
VERSION     DAOSOL010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63312_length_1846_cov_3.838079"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..1496
                     /gene="purF"
                     /locus_tag="PK171_06400"
     CDS             99..1496
                     /gene="purF"
                     /locus_tag="PK171_06400"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT05852.1"
                     /translation="MTGEKCGIFAAYGVKNAAELIFHGLYSLQHRGQESAGMVISDQH
                     GVREHKGMGLVSEVFQGDILKKLTGSIGLGHVRYSTTGSSSLRNIQPFIGDCRFGNVS
                     IAHNGNLANTNSLWKKIGHQGAVLQSTMDTEMILHLIAQSSFDGFEDAVKEALWEIRG
                     AFSLGIMSEKNIIAIRDPWGFRPLALGKLDGGYLISSESCAFDIIGAQFIREIEPGEM
                     LIIDENGPRSVFYAHSHRKAFCIFELIYFARPDSIIFGESVYLARKKMGWVLAEREDY
                     DADMVIPVPDSGIYAALGFSEKSKIPFELGMIRNPYVGRTFIRPGRENRNLAVKLKLN
                     PLKSLLKGKKIIILEDSIVRGNTSRERIKTLKNAGVKEVHMRVTSPPHCHPCYYGIDF
                     PTREELIACRMNQEEIRQFLGLNSLRYIDIDGLKKSLSKPGENFCFACFTGDYPVVPD
                     KGLGKFILEENSSEQ"
     gene            complement(1512..>1846)
                     /locus_tag="PK171_06405"
     CDS             complement(1512..>1846)
                     /locus_tag="PK171_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT05853.1"
                     /translation="LVDYDLIILGTPTYYGTMAAEIKKLLDDSIRLHGRLDGKLGGAF
                     ASSANAAGGNETAILSILSALLIHGMLVKGMAEGSHYGPVAIKSFDDRAEKECRSYAN
                     QLADILKD"
BASE COUNT          555 a          365 c          399 g          527 t
ORIGIN      
        1 cctctcacca aatttaaaga agagttttcg gggcttaaaa aaccaagata tcggatggcg
       61 gattaataaa tcatgaccat ctttaaatgg aggaatctat gaccggagag aaatgcggaa
      121 tttttgccgc ctatggagta aaaaatgctg ctgaattaat atttcatgga ttgtattccc
      181 ttcagcaccg aggtcaggaa agtgctggaa tggtcatttc tgaccaacat ggtgttagag
      241 aacacaaagg aatggggctg gtttcagaag tttttcaagg agatatacta aaaaagctaa
      301 ccggttctat tggactcggg catgttcgtt attccaccac tggctcttca tccttgagaa
      361 acatccagcc ttttattgga gactgtcgtt ttggcaatgt ctctattgcc cataacggaa
      421 atcttgctaa taccaattct ctatggaaaa aaataggtca ccagggggct gttcttcaat
      481 caaccatgga tactgaaatg atcctccact tgattgccca aagttctttc gatggatttg
      541 aagatgcggt gaaagaagca ctttgggaaa tccgaggagc tttttccttg ggaataatga
      601 gtgaaaaaaa tatcattgcc atccgcgatc cctggggttt tcgacctttg gctttaggaa
      661 agctagatgg aggctacctg attagttcgg aaagttgtgc ttttgatatc attggagctc
      721 aattcattcg agaaatcgaa ccaggcgaga tgctgataat tgacgaaaac ggtccaagat
      781 cagtttttta tgcccattct catcgaaaag ctttttgtat atttgagctc atttattttg
      841 ctcgaccaga cagtatcatt tttggtgaaa gtgtctattt agcacgaaaa aaaatgggat
      901 gggttttggc agaaagagaa gactatgatg ccgatatggt tattccggtc cccgattcag
      961 gaatttatgc tgcactagga ttttcagaaa aatcaaaaat tccttttgaa ttgggaatga
     1021 ttcgaaatcc ttatgttgga aggactttta tacgtccggg gcgagaaaac cgtaatcttg
     1081 cagtgaagct gaagttaaat ccgttgaaaa gtcttttaaa agggaaaaaa ataattattc
     1141 ttgaagattc cattgtaaga ggaaacacct caagagaaag aattaaaacc ctaaagaacg
     1201 ctggggtgaa agaagttcac atgcgtgtca cttcaccccc tcactgccac ccctgctact
     1261 atggaatcga ttttccaacc cgggaagagc tcattgcctg ccgcatgaac caggaagaaa
     1321 ttagacaatt ccttggtttg aatagtcttc gctatatcga tatcgacggc ctcaaaaaga
     1381 gcttatccaa accaggagag aacttttgtt ttgcctgttt cactggtgac tatccagtag
     1441 tacccgataa gggattagga aaatttatct tagaagaaaa ttcctcggag caataatgta
     1501 ccttattgat attaatcttt taaaatatcc gccagctgat tcgcgtagga tcggcattct
     1561 ttttctgctc ggtcatcaaa agatttaata gcaactggtc cgtagtggct tccttctgcc
     1621 attcctttga ccagcattcc atgaatgagg agagccgaga gaatagaaag aattgcagtt
     1681 tcatttcctc cggcagcatt agccgacgaa gcaaaagcac cacccaactt gccatccaat
     1741 cgaccgtgca agcggatgct atcatccaaa agctttttaa tttctgctgc catggttcca
     1801 tagtaagtcg gtgtaccaag aatgatcagg tcataatcga ccagct
//
LOCUS       DAOSOL010000296         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_63444_length_1844_cov_2.417552, whole genome shotgun sequence.
ACCESSION   DAOSOL010000296 DAOSOL010000000
VERSION     DAOSOL010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63444_length_1844_cov_2.417552"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..322
                     /locus_tag="PK171_06410"
     CDS             104..322
                     /locus_tag="PK171_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05854.1"
                     /translation="MLSDYKGQLLFGQKVAGGERIQLLTIPFRGSHDMILHLEGTMKG
                     KSEGLVLVRHISNPEIIQTIKIPSVIVQ"
     gene            complement(527..886)
                     /locus_tag="PK171_06415"
     CDS             complement(527..886)
                     /locus_tag="PK171_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOT05855.1"
                     /translation="MERLVKGDIVVLPFPFSDLSQSKKRPAFVVAPLQGDDVILCQIT
                     TVIHSDLYSIFLEEKDFVTGSLKQPSYIRPNRLFTSDIRIIIKRVGSVHQNKIEEVIK
                     KITMIITGPSVESGLNS"
     gene            complement(871..1107)
                     /locus_tag="PK171_06420"
     CDS             complement(871..1107)
                     /locus_tag="PK171_06420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT05856.1"
                     /translation="MNQKVSVVDRRGRLTIPGSLRKKMGVKEGDIFLIREEEERLVCE
                     KISVSSSFQMMQASERVLSRDWDTPEEDEAWKDL"
BASE COUNT          581 a          390 c          310 g          563 t
ORIGIN      
        1 aggaaatcga gttacatgtt gaattagata agaccaaaga tcctttgaat actatgcaat
       61 tatagttgct ccctcggttc tatctatccg tcaaaccgag gacttgctct ccgactataa
      121 ggggcaactg ctttttggac agaaagtagc tggaggagaa agaattcaac tcctgacaat
      181 tcctttccgc ggatctcatg atatgatact ccatctggaa ggaacgatga aaggtaaaag
      241 cgaagggctg gttctggtac gacatatttc taatccggaa attatccaaa ccatcaagat
      301 tccaagcgtt atcgtacagt aataaaaaga agcctgtgcc aatttcttaa cacaggcttc
      361 tttttatcgg cttattttgt gttaatgtca ttaacttcga gcaccacaac gtagtcaatc
      421 ctaagcccgg tttccttctc ctttttctct ccctctcccc ctcagaatct aatggcgtga
      481 ggatctcatc ttttattttt ccctagtgtc ccaattcccc tgaaattcaa gaattaagtc
      541 ctgactccac cgatggtcca gtgataatca tggtgatttt cttgatgact tcttcaattt
      601 tattctggtg aactgatcca acccttttga taataatgcg gatatcgctg gtaaacaaac
      661 ggttagggcg gatataactc ggttgtttca agctaccagt aacaaaatct ttttcttcca
      721 ggaaaattga atagagatcg gaatgaataa ccgtggtaat ctgacaaaga atcacatcat
      781 caccttgcaa tggagcaaca acaaaagccg gtcgcttttt gctttgactc agatcagaaa
      841 agggaaaggg aagtacaacg atatctcctt ttacaagtct ttccatgctt catcctcttc
      901 aggagtgtcc caatctcgac tcaatactct ttctgaagct tgcatcattt ggaatgaaga
      961 ggaaactgag attttctcgc aaaccaatct ttcttcttct tctctaataa gaaagatatc
     1021 tccctccttc acacccattt tctttcggag tgatcctgga atagtcagac gaccccgacg
     1081 gtctacaacc gataccttct gattcatatg tatcctccta agtaggaatt acccacaaaa
     1141 tattatagca tttttccatt atttaaaaaa agcgataaaa aaatatatct taacaatcaa
     1201 atctattttt tttgcattaa ttgactgaaa cgagagaaca cataagcaca ctaagttaag
     1261 gagatgaaat tataaatatg accagttata agaacagttc aacagatttt tgagatgagc
     1321 aagaaaataa aaagctaaat acttaaacat gagaggcata aagagaagat tacaaattga
     1381 agagattcat aaagattttt tgagggaaat cgttcaatac aaaaatgtcc ataaacgcta
     1441 aacttaagat tctaccaata tttctaagga actcatcaac ctaatccgtc attgcgagac
     1501 ctcgtttttt tttgaggtcg tggcaatctc atgagtcttc tttaattatt tgtagaactg
     1561 agaaatcatg gaattataaa ggagaggatt cttacctcgt ctgacaaaat acactgaact
     1621 ccgcagtatg acaaattaaa aaataccgtc atcctgagga ttcttttttc gaatccgtga
     1681 ggacctcatc ttttattttt ccctagtgcc ccaattcccc cgaaattcaa gaatcaagac
     1741 ctggtccctt caaattctgt ttacctcgtt atcctcttta aattacatga tagacccccg
     1801 gggatttcgt tagagttcct ctaataactt tctaaactgt tcag
//
LOCUS       DAOSOL010000297         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_63812_length_1836_cov_3.151600, whole genome shotgun sequence.
ACCESSION   DAOSOL010000297 DAOSOL010000000
VERSION     DAOSOL010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63812_length_1836_cov_3.151600"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PK171_06425"
     CDS             <1..330
                     /locus_tag="PK171_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05857.1"
                     /translation="FSKDFFQSAYDHLSDNGVLVSQIEPPFFQPEREQRHWESLKMFP
                     LVKIYWGLVPTYPGGIWTYMIASKGRDPETSFKKLLFSTRYYTPEIHRAAFVLPPFMQ
                     ERLSKKL"
     gene            complement(914..>1836)
                     /gene="hisD"
                     /locus_tag="PK171_06430"
     CDS             complement(914..>1836)
                     /gene="hisD"
                     /locus_tag="PK171_06430"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOT05858.1"
                     /translation="SVATYVPGGRASYPSSVIMTVVPAQIAGVKNILLLTPPNSKGEV
                     SSNILAVAHYLGISNIVRAGGAQAIAAIAFGTESIPRVDKVVGPGNIYVTVAKRLLCG
                     IIGIDSLAGPSEVVIIADEECRPDWVALDLLAQAEHDPLSRAILFSTSDEVINQVQEV
                     IQSELNAHPLPFTKAVPMYLFKVDNLLAAFEITNKIAPEHMELCCADHLRLLPLIRHA
                     GAIFLGNGAPVALGDYGYGPNHVLPTMSGSRFSSPLSVRDFLKTSSYIFPQKGDPTLV
                     YSRYASLAEKEGFFFHRKSLLARINPHDIV"
BASE COUNT          551 a          373 c          397 g          515 t
ORIGIN      
        1 ttttcaaaag attttttcca gagtgcttac gaccaccttt ctgataatgg tgtattagtt
       61 tcccaaatag aaccaccttt tttccaaccg gaaagagagc agagacattg ggaaagttta
      121 aaaatgttcc ctctggtgaa gatatattgg gggttggttc caacctatcc cggtggcata
      181 tggacctata tgattgcatc aaaaggaagg gatcctgaaa ctagctttaa aaaacttcta
      241 ttttccactc gttattatac tccagaaatt catcgggcag cttttgttct tccgcctttt
      301 atgcaagaaa ggttgtctaa aaagctataa cgcatgtcga gctttatact ggttgggcaa
      361 ggacacgaag tggcatgagg tttaagtctg aaaaaccacg agcttgattt gttgcgtccg
      421 gtcttcattg tatgtagcga cctgccatgg catgtcgaat ctttggtttt cattgtcatc
      481 tggtgccaca caagtggcat gaaggtctat cctgaaaata ccatcaaatg aattctttaa
      541 tccgtcatcc tgagccctca ctttgtgagg gcatgaagat ctcatctttt aattatttta
      601 aaaaaaatac taaatgagat tgccacgtcg cccaaccaaa gaacggttgg tctcctcgca
      661 atgacggaac agtaaaaatc aaatcccact tacccccttt tctaaagggg gaaattgatc
      721 ggcgtataga tatttttatc ctcatttggt gctgcaaagc agcatgaagg tctatcctga
      781 aaagccacgg gcatgataaa tcaagcccct acaaaagatt aaaaaattgt aggggcacaa
      841 tgcattgtgc ccattcttta attaatatag cgacatggca tgtcgaatct tggatttaaa
      901 tcctttttta gtactacaca atatcatgag ggtttattct ggctaacaga gattttcggt
      961 gaaagaagaa accttctttt tccgccaagc tggcatatcg agagtaaacc agagtaggat
     1021 ctcctttttg agggaaaatg taggaagaag tctttaaaaa gtcacgaacc gaaagaggac
     1081 tagagaaccg acttccactc atggttggta aaacatgatt gggcccgtac ccataatcac
     1141 caagtgccac gggcgctcca tttccaagaa atatagctcc agcatgacga atcagtggta
     1201 acaaacgaag atgatcagca cagcataatt ccatgtgttc aggagcgatt ttgttggtta
     1261 tttcaaatgc cgctaataaa ttatcaactt tgaatagata cataggaaca gctttggtga
     1321 agggtagagg atgagcgttt agctcgcttt gaataacttc ttgtacctgg tttataacct
     1381 catcactggt tgagaataat attgcccggg agagaggatc atgttcggct tgagcgagaa
     1441 gatcaagagc aacccagtcg ggtcgacatt cttcatcagc aatgatgact acttctgaag
     1501 gaccggctaa tgaatcaatg ccgatgatac cacacaaaag ccttttagcg acggtgacat
     1561 aaatatttcc tgggccgaca actttatcga ctcggggaat agattcggta ccaaaagcga
     1621 ttgcagcgat agcctgagca cccccagctc ggactatgtt gctgatacca agataatgag
     1681 caactgctaa aatgtttgat gaaacttcac cttttgaatt cggaggagta agaagaagga
     1741 tattttttac tccggctatt tgggctggga ctaccgtcat aattactgac gatggataag
     1801 aagctctccc gcctggtaca taagttgcta ctgagt
//
LOCUS       DAOSOL010000298         1832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_64023_length_1832_cov_3.077659, whole genome shotgun sequence.
ACCESSION   DAOSOL010000298 DAOSOL010000000
VERSION     DAOSOL010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64023_length_1832_cov_3.077659"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..785)
                     /locus_tag="PK171_06435"
     CDS             complement(273..785)
                     /locus_tag="PK171_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT05859.1"
                     /translation="MKLQTKKIVLAGVLGAISIILSVTPIGLIPVPNLSGSATTMHIP
                     AIVGGIIGGPLVGALVGLILAFSTMNLFFAMGVNLVACFVPRILIGVVAYYIWAGLKK
                     GNIGIIASSLGATFVNTIGVLGLAVLFGNFTLQQIAPIIALNGTLELIFSAIIVLPLV
                     KILQRQFPSK"
     gene            complement(948..>1832)
                     /locus_tag="PK171_06440"
     CDS             complement(948..>1832)
                     /locus_tag="PK171_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOT05860.1"
                     /translation="VYTGSTVFLFERKNRAIASVRGGAPGSRELNALSPGQLVENIDA
                     LVFSGGSSFGIDSMSGAVEYLHQKGVGYKTKAANIPIVAGAVIYDLEVGKNAVPHSEW
                     VYAACEDATEKPKEGTVGAGTGASVGKSSGIRFAMKSGFGWGKSPHHRDSIHTFIVTN
                     ALGDIYDPRNGQLLAGRRNKSGEIEGFLKNIQNDTGFTSYYNTTLALVVLDYYFSRED
                     LLMLSHVVHSALSTCIRPYATLFDGDTIFMVSMGDRRPNYLSILQGIYESVTEAVIHS
                     ILFAEGLPDLPSRNDIIK"
BASE COUNT          585 a          398 c          309 g          540 t
ORIGIN      
        1 tgaattcaac aattcccctc cttggagggg tgccgtttta cggcggggag ggtgcttttt
       61 atcagtcatc ctgaggcttc gtattcagaa gccgtgagga tctcatcctt taattatttt
      121 taaaaaaaat ttgctaaatg agattgccac gtcgcacaaa acgctccttg caatgacggt
      181 tttagatagc tttttcatcc ccatctggca caatcgaagt ggtttggaag tctatcctgt
      241 tttggtattg agacaaaaac taagtaagtt tttcattttg aaggaaactg gcgctgtaat
      301 atttttacca aagggagaac aattattgct gaaaaaatta attctaatgt tccatttaaa
      361 gcaataattg gagctatctg ctgtagggta aaattcccaa acaaaacagc taaacctaag
      421 actcctattg tgttaacaaa ggtagctccc aagcttgacg caataatacc aatatttcct
      481 tttttcaaac cagcccaaat ataataagca accacaccga ttaatattcg aggaacaaaa
      541 caggcaacga gattaacccc catggcgaag aataaattca ttgtagaaaa agcaaggata
      601 agaccaacca aagcaccaac taatggacca ccgattattc caccaactat tgccggtata
      661 tgcattgtcg ttgcagatcc tgagagattc ggtaccggta ttaacccaat aggagttacg
      721 ctgagaatga tagaaattgc ccccaacaca ccggctaata ctatcttttt cgtttgaagt
      781 ttcatgatta tccctccgtt ctagttctct tctgagatac cagacggata aagttttctt
      841 tatccatctc tcgccgccat tttggtcgac gggttaaaca aaattcattc ctattttcga
      901 ataagctaat acatccttca ttcagtatag gcttcaactc tatcaattta ttttattata
      961 tcatttcggg aaggtaaatc aggtaatcct tcagcgaata aaattgaatg gataactgct
     1021 tcggtaacac tttcatatat accttgaaga atactcagat agttgggacg ccgatcaccc
     1081 attgaaacca taaaaatggt atccccatca aaaagagttg catagggtcg aatacaagtt
     1141 gataaagcgg aatgaacaac atgagaaagc attaaaaggt cttctcttga aaaataatag
     1201 tccagaacaa ccaaagccaa ggtagtatta taataagatg taaaaccagt atcattttga
     1261 atatttttca aaaagccttc aatttctccg cttttgtttc ttctgccagc taataattgt
     1321 ccatttctag gatcataaat gtcacccaat gcgttggtaa caataaaagt atggatacta
     1381 tctcgatggt gaggggattt tccccatcca aaaccactct tcatagcaaa tcgaattcct
     1441 gagctttttc ctaccgaagc tccagtccca gcacctaccg ttccttcttt gggtttttct
     1501 gttgcatcct cacaagcagc ataaacccat tctgaatgag gaacagcatt ttttccaacc
     1561 tctaaatcat agataactgc accagcaaca attggaatat tggcggcttt ggttttatat
     1621 ccaactcctt tttggtgtaa atattcaacc gcccctgaca tactatcaat tccaaaagag
     1681 ctacctcccg aaaatactaa ggcatcaata ttttcaacta attgcccggg agaaagagca
     1741 tttaattctc tacttccagg cgcaccccca cgaactgagg ctatagcacg attttttctt
     1801 tcaaaaagaa atactgtact tccggtataa ac
//
LOCUS       DAOSOL010000299         1827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_64256_length_1827_cov_2.893341, whole genome shotgun sequence.
ACCESSION   DAOSOL010000299 DAOSOL010000000
VERSION     DAOSOL010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64256_length_1827_cov_2.893341"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PK171_06445"
     CDS             complement(<1..858)
                     /locus_tag="PK171_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05861.1"
                     /translation="MGKPVKQQNSFLKKLLTTRSFSILVVLIALLIIFTVFSPGGNFI
                     NAENIKVLLAYGSEFSIIALGVGVLMIAGEFDLSIGSILVFCSYLFLIFFKLNLNYFV
                     VLALTILGGIALGYINAIVTTKGHIPSFITTLGTMMLWRGLTLWFSGGLQQACDVSSS
                     PAFLSFLNGTIGGVFPVQALWFIIVGIILGLLLHYTRYGNWIYATGDNADAARAMGIR
                     THIVKMTAFIIVGILCAFVAGMQISRVSCFTSRTGEGWELKAMAACVVGGTSLRGGIG
                     DMAGIFLGAF"
     gene            complement(885..1643)
                     /locus_tag="PK171_06450"
     CDS             complement(885..1643)
                     /locus_tag="PK171_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05862.1"
                     /translation="MTTEANQNSDMVKMVDIHKWFGRVYALRGIDFNLKRGETLGLLG
                     DNGAGKSTLIKILSGYHVADRGEIYFDGKKALIHSPRDARKLGIETVYQEQALAPNLS
                     ISRNIFMGREPLKGLGLLDKKVMDEESMQALKHLGLRLRSPDVPIITLSGGERQGVAI
                     ARAIHFKAKCVILDEPTVALSVKEVNEVLEFIRQIKREGISSIFITHNLYHVYDIAER
                     FVVMARGRKMADVKKEEVTRDNLSDLVIKSAMVE"
BASE COUNT          586 a          421 c          319 g          501 t
ORIGIN      
        1 aaaagcaccc agaaaaatac ctgccatatc gccaattcca cctcgtaggg atgtccctcc
       61 gacgacacag gcagccattg cttttagctc ccaaccttcg ccggttcggg aagtaaaaca
      121 acttaccctt gagatctgca ttccagccac aaaagcgcac aaaattccaa caatgataaa
      181 tgctgtcatt tttactatat gggttcgaat ccccatagcc ctggctgcgt cagcattatc
      241 tccagttgca tagatccagt ttccatagcg ggtgtaatgg agtaacaatc ccaaaataat
      301 tcctacaata ataaaccaca aagcttgaac cggaaatacg cctccaatgg ttccatttaa
      361 aaaggaaaga aaagctggcg acgaagaaac atcacaagct tgttgcaaac ctccggaaaa
      421 ccagagggtt aatcccctcc ataacatcat ggttcctaag gtagttataa aggatggaat
      481 atgacctttg gttgtgacta tggcattgat atagccaagg gcaatacccc ctaagatggt
      541 caatgctaag acgacaaaat aattaaggtt aagtttaaaa aatatcaaga aaagatatga
      601 gcaaaaaacc aggatggatc caatcgacaa gtcaaactct ccggcaatca ttaggacgcc
      661 gactcctaag gcaataatac tgaattctga accgtaagcc aggagtacct ttatattttc
      721 agcattaata aagttaccac cgggagaaaa aactgtaaat ataatgagaa gcgctatcaa
      781 aaccaccagg atactaaacg accgggtggt taataatttt tttaaaaaac tattttgctg
      841 tttcactggt tttcccattg ctcctttctc ccagaaattc cattttattc aaccatagca
      901 ctttttatta ctaagtcaga aaggttatca cgagtcactt cctctttctt tacatcggcc
      961 atttttcttc ctcgagccat aaccacaaac cgttcggcaa tatcatagac atgatagagg
     1021 ttgtgggtaa taaaaatgga tgatattccc tcacgtttga tttgacgaat gaattctaaa
     1081 acttcattga cttcttttac cgaaagagcc acggttggtt catcgagaat gacacatttg
     1141 gctttgaaat ggatagctcg agcaattgca acaccttgcc gctctccacc tgaaagggta
     1201 atgatcggga cgtcaggtga ccgtaaacga agccctaaat gcttaagggc ttgcatgctt
     1261 tcctcgtcca tgactttttt atccagtaac cccaacccct tcaacggttc tcgacccata
     1321 aatatatttc ttgaaatact taaattggga gctaaggctt gttcttggta aactgtctca
     1381 atcccaagct ttcgtgcatc acggggagag tgaatcaggg ctttttttcc atcaaaataa
     1441 atttctcccc tatcggcaac atgataccca gaaagaattt tgatcaaggt cgatttacca
     1501 gcaccattat ctcctaataa acctaaggtt tcacctcgtt tgaggttaaa atcaatccct
     1561 ctcagagcgt atacccgacc aaaccattta tggatgtcga ccattttcac catatcacta
     1621 ttctgattgg cttctgttgt catcggttaa acctcacttt aaaagatgtg aattacctca
     1681 tttttctttc aatgtaagta tttagcaaaa caaacagaac ggttgctaaa ccaatgaaag
     1741 cactaatagc aaacaccgga actctcatga ggatgaggcc attatcaatg attggaataa
     1801 taattgcccc taaaactatt cctaaca
//
LOCUS       DAOSOL010000300         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_64691_length_1818_cov_3.305729, whole genome shotgun sequence.
ACCESSION   DAOSOL010000300 DAOSOL010000000
VERSION     DAOSOL010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64691_length_1818_cov_3.305729"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PK171_06455"
     CDS             complement(<1..264)
                     /locus_tag="PK171_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT05863.1"
                     /translation="MQYYHSFINGKWEEGEGFFEVINPSNGEVIAAVTKVKLYQMKDA
                     INSAHAAFKIWSKKLAIERSRLLFKAAEKVRERAAEMGKLLAKE"
     gene            complement(267..1769)
                     /locus_tag="PK171_06460"
     CDS             complement(267..1769)
                     /locus_tag="PK171_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOT05864.1"
                     /translation="MPECFFLVVDIGTESVRAALIDSLGAIVGIQSRSTDFFSPHPGW
                     AEQRPDEWWELTIECLKQLMVEHPGIEVVAIGVSAQMHAVVPIDSRGTLLMDKVPIWC
                     DKRSTDICQQYQKELLPKEQIQLTANLIIPSWTGPKMKWIKDNLPQVYQQTEIFLTAK
                     DYLNFRFTGEKYTDFSEASGSFLFSWKTKQWDNELIKMFGIDKLKLPEILPSNAIIGK
                     LLPDIAAQIGCQPAIPVICGAGDMLCLLLGGGMVREGRSCDVTGTAADVSVLSPKPLL
                     TEHLMNLHHAIDGWISFGILDSGGGSLKWFREAFYHQEDGRKMSYAQIDDEASDVPAG
                     ADGLLFFPYLMGERLLGSPSARGSFFGLRPHHQRKHFARAVMEGVCFDLKMSLDEIEE
                     RYSGTIEEMSVIGGGARSSLWCQLKANIYQKKVFTLAESEGGIIGAALLAFSALNGEP
                     VDEIAEKWLKVKQVYVPDESRFDEYQKQYKNFRLFHDIFQQAYLHFGKEN"
BASE COUNT          546 a          441 c          312 g          519 t
ORIGIN      
        1 ctctttagct aatagcttcc ccatttcagc tgctctctca cggacttttt ctgctgcctt
       61 aaatagcagc cgactcctct cgatagcgag tttttttgac caaattttaa aagcagcatg
      121 agcactatta atggcatctt tcatttgata cagcttcact ttggttactg cagcaataac
      181 ttcaccattg gatggattaa taacctcaaa gaatccttct ccttcttccc attttccatt
      241 tataaaagaa tgataatatt gcacttttag ttctcctttc caaaatgaag ataagcctgt
      301 tggaaaatat cgtggaacaa ccgaaaattt ttatactgct tttgatattc atcaaaccgc
      361 gattcatcgg gaacgtaaac ctgttttact tttaaccact tttcagctat ctcatcgacc
      421 ggctctccat ttagggcaga aaaagccaac aaagccgctc cgatgattcc accttcggat
      481 tcagccagag taaatacttt tttttgatag atattggctt tgagctgaca ccataaagaa
      541 ctccgggcgc cccctcctat cactgacatt tcttcaatag ttcctgaata ccgctcttcg
      601 atctcatcca agctcatttt taaatcaaaa caaacccctt ccatgactgc tcgagcaaaa
      661 tgctttcgct gatgatgagg gcgcaaacca aaaaaactac cccgtgccga gggagaacct
      721 aatagtctct cccccattaa ataaggaaaa aataataagc catcagcacc agctggaaca
      781 tcactggctt catcatcaat ctgagcatag gacatttttc taccatcttc ctgatgataa
      841 aaagcctctc gaaaccactt taagctacca ccaccgctat ccaaaattcc aaaactaatc
      901 caaccatcaa tagcgtggtg gagattcatc aaatgttcgg tcaaaagtgg ttttggactc
      961 aacaccgaaa catcagcagc agttccggtt acatcacagg aacgcccttc tcgaaccatt
     1021 ccccctccta aaaggagaca caacatatca ccagctccac aaatcaccgg gatagcaggt
     1081 tggcagccaa tttgagcagc gatgtctgga agtaatttac caattatcgc atttgaaggt
     1141 aaaatttcgg gaagtttcaa tttatcaatc ccaaacatct taatcagttc attatcccat
     1201 tgtttcgttt tccaggaaaa aagaaaacta cctgatgctt ccgaaaaatc agtatatttt
     1261 tcaccagtaa aacggaagtt taggtaatct ttcgccgtta ggaaaatttc agtttgttga
     1321 tagacctgag gaagattatc tttaatccat ttcattttgg ggccagtcca actcggtatg
     1381 atgagattgg cagttaactg aatctgttct ttgggtaata actctttttg gtattgttgg
     1441 catatatcgg ttgaacgttt atcacaccag attgggacct tatccatcaa caaagtccca
     1501 cgagaatcaa ttggaaccac tgcatgcatt tgggcactga ccccgatcgc tacaacttca
     1561 atgccgggat gctcaaccat gagttgtttg aggcattcta tggttaattc ccaccactca
     1621 tcaggtcttt gttcagccca gccaggatga ggtgaaaaaa aatctgtaga ccgggattga
     1681 ataccaacaa tagcacccaa actatcaatc agagcggctc tcacgctctc ggtaccgata
     1741 tctacaacca ggaaaaaaca ttctggcatg atgatatcaa ccttccttat tgatggttat
     1801 ccatacgtaa gaaagggg
//
LOCUS       DAOSOL010000301         1793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_65924_length_1793_cov_4.340046, whole genome shotgun sequence.
ACCESSION   DAOSOL010000301 DAOSOL010000000
VERSION     DAOSOL010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65924_length_1793_cov_4.340046"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="PK171_06465"
     CDS             <1..1530
                     /locus_tag="PK171_06465"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05865.1"
                     /translation="PTLMRKGFFMSTPVLECRQICKSFGGVTVLNNVDFILEKGEIHA
                     LVGQNGAGKSTLMKIINGVYTRDSGDILIEGKEQVYKSAQEARKAGISMVFQDFSLVP
                     TLTVSQNIFLARELSGGRVVLDDRRMEEITQKLLDSIGIDISIHPREHVENLSVGSKQ
                     LVEIAKALSTDSKILIFDEPTASLSQAEISSLFSVIQNLKKKGISIIYISHYLKDIFK
                     LCDSVTVLRDGVNVLSRPISETSLEEVIDAMVGKKVGNRASWERSQRKEEEKPLLSVR
                     NLSTNSIHQISFDLWPGEIVGLAGLLGSGRTEIFKALFGLDPIKSGEIFIQGKKSTIL
                     SVPDALKYGLGLVPEDRRNQGLIMDFSINENILLPLMNKLTQLILINDHRGKEISQKF
                     VSDFKVKCDGIEQQVRYLSGGNQQKVVVAKNVANQPKILLLDDPTFGVDIQSKWEIMN
                     IVKEFARLGNGVLFISSEFGEVASFCDRVLVVKKGEITETIVNSDEQTLDEEVLIKMV
                     Q"
BASE COUNT          546 a          296 c          398 g          553 t
ORIGIN      
        1 cccactttga tgagaaaggg tttttttatg tcaacgccgg ttttggaatg ccgtcaaatc
       61 tgtaaatctt ttggtggggt aaccgttcta aacaatgttg actttattct tgagaaaggt
      121 gaaatacatg ctttagttgg acagaatggt gccggcaaat caaccttaat gaagattatt
      181 aacggggtct atactcgaga tagtggtgat atcttaatcg aaggaaaaga acaggtttat
      241 aaatctgctc aagaagcgcg caaagccggg ataagcatgg tatttcaaga ctttagtctg
      301 gtgcctaccc tgactgtttc gcaaaatatt tttttagctc gagaattatc aggtgggcgt
      361 gtcgtgttag acgatcgaag aatggaggag ataacccaaa aacttcttga ttcgattgga
      421 atcgatattt ccattcatcc ccgggaacac gttgaaaatt tgagtgtcgg ttccaaacaa
      481 ttagttgaaa tagcgaaagc cctttcaacc gattcaaaga tattgatctt cgatgaacct
      541 accgcatctc tctcccaagc cgaaatttca tcattgttca gtgtgattca aaatctgaaa
      601 aagaaaggga tttcgattat ttatatttcc cattatttaa aagatatttt taaattatgt
      661 gactcagtta ccgttttgcg agatggagtc aatgttttat cccgaccgat ttcagaaact
      721 tctttagaag aagttattga cgctatggtt ggaaaaaagg tggggaatcg ggcaagttgg
      781 gagagaagcc agagaaaaga agaggagaaa cctctcttat cggttcgaaa tctttccact
      841 aattcgattc accaaatcag ttttgatctt tggcctggtg agatagttgg attggctgga
      901 ctattaggaa gtggacgtac tgaaattttc aaagctcttt tcggcttgga tcctattaaa
      961 agcggagaaa tatttattca gggtaaaaaa tctacaattc tttcggttcc tgatgctcta
     1021 aagtacggtc ttggtttagt cccagaggat cggcgtaatc aaggtcttat catggatttt
     1081 tccattaatg aaaatatctt gcttccccta atgaacaaac tgacccagct cattttgatc
     1141 aatgatcacc gggggaaaga aatcagtcag aaatttgtta gcgattttaa ggtgaaatgt
     1201 gatggtatcg aacagcaggt tcgttatctt tccggtggta atcaacaaaa agttgtcgtg
     1261 gcaaaaaatg tagctaatca accaaaaatt ctcctcctcg acgatcctac ttttggagtg
     1321 gatattcaat ccaagtggga aattatgaac attgttaaag aattcgctcg attggggaat
     1381 ggtgttttat tcatttcttc cgagtttgga gaagtagcct ctttctgtga tcgagtgttg
     1441 gtggttaaaa aaggagagat aactgaaact attgttaata gtgatgaaca aacccttgat
     1501 gaagaagttc tcataaagat ggtgcaatag gttttattgg ttttcagatt gatttaattt
     1561 tttattcata atccatgcta agtcattatt tagttccttc tcccttgatg ggagaaggcg
     1621 aggatgaggc tgaaaggttg gatttagaaa caagttttgt gttttaaatt gaacttgagg
     1681 aaaatatcca aatatccccc tcacctgaat cctctcccac cagaggagag gaaaaaaaga
     1741 gaaatgtgag aaggtcagga tgagaatctg gtaagctcca tgtcatgaca tgg
//
LOCUS       DAOSOL010000302         1787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_66247_length_1787_cov_3.254619, whole genome shotgun sequence.
ACCESSION   DAOSOL010000302 DAOSOL010000000
VERSION     DAOSOL010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66247_length_1787_cov_3.254619"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PK171_06470"
     CDS             complement(<1..247)
                     /locus_tag="PK171_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT05866.1"
                     /translation="MSSQSLFSLKDEVAVVTGGAQGLGEQMAIGLAEAGAHVIIADLN
                     LDSAKKVAQSLEKYGAKTMAVHVDVTQKPLVENMVDEI"
     gene            complement(305..381)
                     /locus_tag="PK171_06475"
     tRNA            complement(305..381)
                     /locus_tag="PK171_06475"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Pro,seq:ggg)
     gene            complement(949..1359)
                     /locus_tag="PK171_06480"
     CDS             complement(949..1359)
                     /locus_tag="PK171_06480"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOT05867.1"
                     /translation="MENIKKIALVAHDNQKQDLIEWAQWNKNILDNHLIFATGTTGKL
                     LIDRVGLKVHCLKSGPLGGDLQIGAKIVEGEIDVLIFFWDPLKPQPHDVDVKALLRIA
                     VVYNIPTACNRSSADYLVSSVLLYSQYQPLLKTC"
BASE COUNT          533 a          378 c          304 g          572 t
ORIGIN      
        1 cgatttcatc caccatattt tcaactaaag gtttttgagt cacatcgacg tgaactgcca
       61 tggttttagc gccatatttc tctagtgatt gagcaacttt cttggcgcta tccagattta
      121 aatcggctat aatcacatgg gcaccggctt cagctaaacc aatagccatt tgttcaccca
      181 aaccttgagc gcctccggta acaacagcta cttcatcttt taggctaaaa agtgattgag
      241 agctcaccta taaatcctcc ttgagcagaa gtgttctgtt attggttttt aaaaactgaa
      301 gacttggtcg gggagagcgg atttgaaccg ccgaccactc gcaccccaag cgagtgcgct
      361 cccatgctgc gctactcccc gaaacttacg tcccctcatt cggctatgtt atatcaaacc
      421 atttactctg tcaacataat cctatcttct tgcaagtata ggtttgaatc atttttttca
      481 tttccagcga tagcaaaacc ttataaaaat gactagattc agagccgctt ttggttaaca
      541 gttcatctcg ataacaatta gaaatatcaa gaagattaag gagttctctc cttaaaataa
      601 agtgagaagg gagttatgag taacgaatag aaaattttca gcttcatccc aacctttttt
      661 aatttttttc tttcctctcc ctttggttaa aaggagatca agataaattc aattttttaa
      721 aattctttca aatccccctt accttctttt ctaaaggggg agattgattt ctgaaaaagc
      781 tatttccgcc ctcatcctca ccttctccca tcaagggaga aggaatcaaa aagtcgcaat
      841 ccctcggtct ttattgcgtt cgattaatgt agcgtcatac catggcatat agaattttgg
      901 gctatcactc atcattgttt ctccagagtg gaaaccaata aaacagaatt agcaggtttt
      961 gaggagaggt tggtattggc tataaagtaa gacggatgaa acgaggtaat cagcagagga
     1021 acgattgcag gcagttggaa tattatatac gacggctatc cgcagtaaag cttttacatc
     1081 aacatcatga ggttggggct tgagaggatc ccaaaaaaag attagaacat cgatttctcc
     1141 ttcaacaatt tttgctccta tttggaggtc acctcccaaa ggaccacttt ttaaacagtg
     1201 gacttttaac cctactcgat caataagtaa ctttccagtc gttccggtag caaaaatcag
     1261 atggttatct aaaatgtttt tattccattg tgcccattcg attaggtcct gcttttgatt
     1321 atcatgggca actagagcaa tttttttaat attctccatt cttcactctc ctatctatct
     1381 ttaatattta aaatacaatt aactgaaatt cactcgacca gacgtatcca ttttctcgtc
     1441 tttttcacaa atgttataaa atagtgacga tggtgacgat agttactcag ttttatctga
     1501 atatttatca cttgaacatt caaccataaa tcttttcata agaaaaaacc tatcaaaata
     1561 ctcatagcat aattttttac ctaccattca atataaaatt agaaaactgt ttgtcatgaa
     1621 tattgataag aaccggctgg tcatggttgc tttcggtttt tctcaaccag ccggtcatgt
     1681 ctcctaatta attcggataa aaccttcttc ttcaacaata gcttgacctt ctttgctaaa
     1741 gaagaagtct atgaaagcct tggtaactcc ctggggaata tcgggag
//
LOCUS       DAOSOL010000303         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_66340_length_1785_cov_4.789017, whole genome shotgun sequence.
ACCESSION   DAOSOL010000303 DAOSOL010000000
VERSION     DAOSOL010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66340_length_1785_cov_4.789017"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            516..1484
                     /locus_tag="PK171_06485"
     CDS             516..1484
                     /locus_tag="PK171_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOT05868.1"
                     /translation="MERSRIIIMENWKKVMEKASLKTIMKTASAQHLVIPAFNIPYLP
                     MMEAVIEALKEIECFALIEVARPDITRFEARSFAAIKKEFDRCKDTSVARLHQDHVPV
                     LDEEGKEVDWKLLVTEAIDLGYDSVMIDGSRLSLEENISISKEVVDIAHPVGVLVEAE
                     LGAVMGHESGPMPPYDEIFASGKGFTSPEEAVYMVQKTGIDWLSVAIGNIHGAITGAA
                     KDQKKVQARLNIEHLKKLVQKTGIPLVLHGGSGVQKEYVLQAIQNGITKINVGTEIRQ
                     TYEKAYKESGNNIQAAQKALKEKTKEILNQYYEMTQTARKIGEALS"
BASE COUNT          588 a          322 c          406 g          469 t
ORIGIN      
        1 attgggaata aataaccaaa aactgaagat aaatgtactg tgtcctccac tttcaagttt
       61 ttcttggtgt acaaaaggga ttgtgacaca gcctgattgc gaggaacgaa gtgacgtggc
      121 aatctcatcc aaccactctg tcattctgag gagcgtctta agcgacgtga gaatctcatc
      181 ttttaaagta tttatgaaga aaaaaaccta aaaatgagat cctaacgcca ttagatactg
      241 agggggagag ggggcgaggg ggagaaaaag aagaagggaa gagtgtccag gatgactgat
      301 taaaaacacc ctccccgccg cgaacacggc acccctccaa ggaggggaat tgttgtattt
      361 attcccccat tgagggggga taaagggggg tgtgcattta tctttttttc atcacttttc
      421 acttattacg gtattttcag aatagaatgt tacaatatta ttattgaggg tatcgctcat
      481 ttttataaat acacaggaga atgagaaggt tcaagatgga aaggagtcga ataattatta
      541 tggaaaattg gaaaaaagta atggaaaaag cttctcttaa aacaataatg aaaactgcaa
      601 gcgctcaaca tctagttata ccggcattta atattcccta tctccccatg atggaagcgg
      661 taatcgaagc acttaaggaa attgagtgtt ttgcactcat tgaggttgcc cgacccgata
      721 ttactcgttt tgaagctcga agctttgcag caataaaaaa ggaatttgat cgatgcaaag
      781 atacttcggt cgctcgtctt caccaagatc atgtcccagt tcttgacgaa gaaggaaaag
      841 aagttgattg gaagcttttg gtgaccgaag caatcgacct tggttatgat tcagtaatga
      901 ttgatggatc gaggttatct ttagaagaaa atatatccat ttccaaagaa gtagtagaca
      961 ttgctcatcc ggtaggggtt ttagtcgagg ctgaattggg agcagtcatg ggtcacgaaa
     1021 gcggtccgat gcccccctat gatgaaatat ttgcttcggg taaaggattt acttcaccgg
     1081 aagaggctgt ctatatggtt caaaaaaccg gaattgattg gttatcagtt gctataggga
     1141 atattcatgg agcgattact ggtgccgcta aagatcaaaa aaaggttcaa gcccgattaa
     1201 acattgaaca cctaaaaaaa ctggttcaga aaactggtat tccgttagtt cttcatggtg
     1261 gttcaggagt gcagaaagaa tacgttttgc aagccattca aaatggaatt actaagatca
     1321 atgtaggaac cgagattcgt caaacttatg aaaaagccta taaagaaagc ggaaataata
     1381 ttcaagctgc acaaaaagcc ttaaaagaaa aaaccaaaga aatcttgaat caatattatg
     1441 aaatgactca aacagctcgc aagataggag aagctctatc ctgaaaatac aacgatgaat
     1501 aaaaagtgat caaacaaaaa taagcagggc aaattcccct tgaaagcccc ttcaatggga
     1561 aaatggatta aacaattccc ctcctcgtac agtggcgctt gcggtgggga gggtatcttt
     1621 ggtcatcctg aggctttgtt ttttgaagcc gtgaggatct catctttagg tttttttctt
     1681 cataaaaact taaaaagatg agattgccac gtcgcctaag acgctcctcg caatgacgga
     1741 gcgggtggat gagattgcca cgtcgcctaa gacgctcctc gcaat
//
LOCUS       DAOSOL010000304         1767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_67314_length_1767_cov_3.044393, whole genome shotgun sequence.
ACCESSION   DAOSOL010000304 DAOSOL010000000
VERSION     DAOSOL010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67314_length_1767_cov_3.044393"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PK171_06490"
     CDS             complement(<1..582)
                     /locus_tag="PK171_06490"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05869.1"
                     /translation="MNFSRFLARRLGYSIFVLIGLSIVVFIIARMVPGDPARAALGPR
                     ASQEAVDKLREKLHLDESLVVQYAFWIKSVFQGDLGESLLTRRQVIEDIKEFLPATLE
                     LALFSGLFMAFFGILLGILSARHSNTWVDNLIRVFAYIGIATPAFVVAIILLLVFGMY
                     YQVFPTIGRLSQWVDAPTKITGLITIDSLLTGNL"
     gene            complement(1517..>1767)
                     /locus_tag="PK171_06495"
     CDS             complement(1517..>1767)
                     /locus_tag="PK171_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05870.1"
                     /translation="YPTFYKADLQGYIQGREMFIHVETAVLRIEVTATGNLSYAGAVI
                     CYSPREIYQEKFQNYFGRMIRSVAKSMETIPTQNKQDG"
BASE COUNT          559 a          371 c          351 g          486 t
ORIGIN      
        1 taaatttcca gtgagcaaac tgtcgatggt gattaaaccg gtaattttgg ttggggcatc
       61 aacccactgg cttaacctac cgatggtcgg aaatacctgg taatacatgc caaagactag
      121 taatagaata atggcaacaa cgaatgctgg agttgctata ccaatataag cgaaaactct
      181 tatcagatta tcaacccagg tattactgtg tcgagccgat agtattccta agaggattcc
      241 aaaaaaagcc ataaataaac cggagaaaag tgccaactca agggtggcgg gtaaaaattc
      301 ttttatatct tcgataactt gacgtcgagt gagtaaggat tcacccaagt caccctgaaa
      361 aactgactta atccaaaagg catattgaac aaccaaagat tcatcaagat gaagcttttc
      421 tcgtagttta tcaactgcct cctgagaagc tcgaggtccg agggctgccc gggcagggtc
      481 tcctggcacc attcgagcta tgataaagac tacaatcgat aatccgatga gaacaaatat
      541 tgaatatccc aagcgtcgag cgagaaagcg tgaaaaattc atactctggt tctacccttt
      601 ccttttatcc tttaaagaca gtgatgaatg attagcaaca gcgatcagta ttgaaaccaa
      661 taatcattaa aataagtact tccatatttc agttttctcc tacccaggag aattaaataa
      721 ccattcattg gctatcatga aaataccatt tcaaacaaat accctattat attggcctct
      781 ccctttggct aaaaggagat tgagagggat tcgattggtt tccaaaaact caaatccccc
      841 taaccccctt ttctaaaggg ggaaattcat ttctgaatga gtaattttca tcctcatctg
      901 gtgctgctag gcagcatgag ggtctatcct gaaatcacac gtacatgata aatcaagttc
      961 ctacaaaaga ttataaaatg tagggcgcaa tttattgcgc ccgatcttca tataatgtag
     1021 cgacatgcca tggcatgtcg aatcttggat tttcatcttc attttatgcc acaacgtagt
     1081 atgaagttct atcctgaaaa taccggaata agtgaaatgt gatgaaacaa aaaagataaa
     1141 gaatgaattc aacaattccc ctccttggag aggtgtggcg gggagggtac cttttggttt
     1201 tttaatggtt tcaataagat aatactaaag aaacagatac aaaagatgag atcctcacgg
     1261 attcgaaaag cgaatcctca ggatgactga gaagctggat gagattgcca cgtcgcatag
     1321 gacgctcctc gcaatgacgg agcagtaaaa attcaaatcc ccctaaccca ctttgttaaa
     1381 gggggagatt ttttctctca cccctcggcg ggagaggcct tattcctttt ttctcattgc
     1441 ccctccgtgg gagagggtta gggtaagggg caaccaattt tcatccacat caagtggagc
     1501 aaagtaacat gagtatttat ccgtcttgct tattttgggt cggaatagtt tccattgatt
     1561 tcgccaccga tcgaatcatc cggccaaaat agttttggaa tttttcctgg taaatttctc
     1621 ggggcgaata acaaataact gccccagcat aactcaaatt acctgtcgct gttacctcaa
     1681 ttctaaggac cgcagtctca acgtgaatga acatctctct cccttgaata tatccttgta
     1741 aatcagcttt ataaaaggtt ggataga
//
LOCUS       DAOSOL010000305         1759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_67699_length_1759_cov_4.499413, whole genome shotgun sequence.
ACCESSION   DAOSOL010000305 DAOSOL010000000
VERSION     DAOSOL010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67699_length_1759_cov_4.499413"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1225)
                     /locus_tag="PK171_06500"
     CDS             complement(74..1225)
                     /locus_tag="PK171_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT05871.1"
                     /translation="MMVNIADDVAKLPRSGIRELMGMALTIPDAIHLEIGEPHFPTPS
                     FILEGLKEFYSKGDIKYTPTVGIHSLRDKIAKKLLKEKNWKVEADDVVILPGSLFGTV
                     VAFRTILDPGDEALIPDPGFTNHFSQVELSGGRIKRYFLLPENNYLPDFESIRKSITP
                     RTKMILVNTPSNPLGVVFPEKVMQSISDLAEEYNLVVVSDEAYEKYIYQGEHFGMYRY
                     VRPERLISIFSFSKTYALTGWRVGYMVVPKDLLIHLMKVSEYMIACTSHLSQKAAEIA
                     LDMPEEEIKKMVDSYRQNCDLCCSGLEKAGFKVYRPGGGYYVWVDIREFSMKSLEFCK
                     KLLQKSHVAVAPGDTFGQSSDGFIRISICRKKEEIEEGIKRIIAARSEL"
BASE COUNT          525 a          407 c          294 g          533 t
ORIGIN      
        1 gtttgtacaa taatttctgg tccgatgcca ctaggatccc ccaaggtaat agcaataacc
       61 ggtaatcttg aattcataac tcactccgag cagcaatgat tctttttatg ccctcttcaa
      121 tttcttcttt tttcctgcaa atagaaatac gaataaatcc atctgaactt tgtccaaaag
      181 tatcacccgg tgcaacggcg acatggcttt tttggagtaa ttttttacaa aattcaagtg
      241 atttcatgct aaattctctg atatctaccc aaacataata tccaccacca ggacgatata
      301 ctttaaaccc agccttttcc aaacctgagc agcataaatc acagttttgc cgataagaat
      361 ctaccatctt tttaatctct tcttcaggca tatctaaagc aatttcagcc gccttttgcg
      421 agagatgaga cgtacaggca atcatatatt cggaaacttt cattaaatga attaatagat
      481 ccttcggaac caccatatac cctacccgcc aacccgttaa ggcataggtt ttagaaaagg
      541 aaaaaatact gatcaacctt tccggtcgaa cataacggta cattccaaaa tgctcaccct
      601 gatagatata tttttcatag gcttcgtcgg atacaactac cagattgtat tcctcggcta
      661 aatctgagat cgactgcata actttttcag gaaaaactac acctaaggga ttagatggag
      721 tattgactaa aatcattttc gttcttggag tgattgactt acgaattgat tcaaaatcag
      781 gcaagtaatt attctctggt aagagaaaat atctttttat tcgtcctccg ctcagttcaa
      841 cctgtgagaa atgattggta aaacctggat caggaatcaa tgcttcgtct ccaggatcta
      901 aaatagttcg aaaagcaact acagttccaa aaagtgagcc agggagaatg actacatcat
      961 cagcttcaac tttccaattt ttttctttca acaacttctt tgctatttta tctcttaagg
     1021 aatggatacc aacagtgggt gtatatttaa tatctccttt ggaataaaat tcttttaaac
     1081 cttctaaaat aaaacttggg gtgggaaaat gaggctctcc aatctccaag tgaatcgcat
     1141 caggaatagt cagagccatc cccatgagtt ctcgtattcc cgaacggggt aatttcgcta
     1201 catcatcagc aatatttacc atcatgacct cctattgaaa agacagtatt ggaatgcttt
     1261 atataagctt catttcaaaa cactaaatct tgaatctcaa tccaggcttt cactctcatc
     1321 tcgacctttt tgctttgctt tcctctcccc tggtgggaga ggattaaggt gagggggcac
     1381 ttagatttct tcaccaagtt aaatttctta acaaccaatc tggatctcaa tctaggaatc
     1441 taaactttca ctctcatctt gaccttctcc catcaagaga gaaggaacct aaatgatgcg
     1501 tcatcctgag cagtgctttt tctcgtgaga atcttatccg acgctaccgg cgtcatcctg
     1561 agacctcgct ttttgagggc gtgaggatct catcttcaag tttttataaa gaaaaaaaca
     1621 aaaaaatgag attgccacgt cgcataaaac gctcctcgca atgacggagc aggtggatgg
     1681 gattcccacg tcgctctgct ccttagaatg tcaccccctc gcgtcctctc ctcccccgca
     1741 gtccatttta catccttat
//
LOCUS       DAOSOL010000306         1747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_68399_length_1747_cov_3.289598, whole genome shotgun sequence.
ACCESSION   DAOSOL010000306 DAOSOL010000000
VERSION     DAOSOL010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68399_length_1747_cov_3.289598"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /gene="lpdA"
                     /locus_tag="PK171_06505"
     CDS             <1..1329
                     /gene="lpdA"
                     /locus_tag="PK171_06505"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT05872.1"
                     /translation="RAGQKGIKTLLIESRDIGGTCLNRGCIPTKAFFRSQEVFHLSER
                     AAEFGVQVKVDGVDWTRIVARKNKVVKQLTGGVRFLLKKAGVEVVDGIGSFLDKQTIE
                     VIEKEKKKETIQAKHILIATGSKSAHLPVPGVDLDGVIDSDEVLDLPSIPEKLVVIGG
                     GYIGMEMACILNTFGTQVEVIEMLPNILPIADSEVVKLLVEILEKRGLKIHTNAKVVR
                     ISKDKKLQVSYEQNSETKVAEGDYVLIATGRVPMTDHLYPEKAGIKIDRKAITTDLQM
                     KTSVPSIFAAGDVNGKYLLAHVAFKEAEVAINNIMGKEATMNYRVVPNCIFCSPEISS
                     VGLTEDEAITSGFDVKVGKFPFRAVGKALIEGETEGFTKIIADQKTGEILGAHIIGPH
                     ASDLIGELTLAMALESTPEEIAHTIHPHPTLTETVMEAADSVFGSSLHLF"
     gene            1375..>1747
                     /gene="gcvH"
                     /locus_tag="PK171_06510"
     CDS             1375..>1747
                     /gene="gcvH"
                     /locus_tag="PK171_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT05873.1"
                     /translation="MNIPKDLLYTPEHLWVKEENGVYRCGITDFAQEELGDLVYVELP
                     KPNLTVKKGDKIGDVESIKTVSNLYSPISGVITEVNSLLENKPEVINQSPYEKGWICV
                     IQSTHPEEIGQLLSSDGYNKVI"
BASE COUNT          556 a          289 c          397 g          505 t
ORIGIN      
        1 cgtgcaggac aaaaaggaat aaaaactctt ttaattgaaa gtcgtgatat tggaggaact
       61 tgtttaaacc gaggttgtat tccaactaaa gcgttttttc ggtcgcagga agtttttcat
      121 ctgagtgaac gggctgcgga atttggagtt caggtaaaag tagatggggt ggattggact
      181 cgaattgtgg ctcggaagaa taaagttgtc aaacaattaa ccggaggagt tcgattttta
      241 ttaaaaaaag ccggagtaga agttgttgat gggattggtt cattcctgga taaacagact
      301 attgaagtca tagagaaaga gaaaaagaaa gaaaccattc aagccaaaca catccttatt
      361 gctaccggtt cgaaatctgc tcatttaccg gttccagggg tggatttgga tggagtcatt
      421 gatagcgatg aagtattgga tttaccatct attccggaaa aattagtcgt gattggcggt
      481 ggttatatcg gaatggaaat ggcctgtatt ctcaacacct ttggaactca agttgaagtg
      541 attgaaatgc tgcccaatat tcttcctatt gccgatagcg aggtcgtcaa gcttttagtt
      601 gaaatattag aaaagagagg cttgaagatt cataccaatg ccaaggtggt tagaatttct
      661 aaagataaaa aactccaagt atcctatgaa caaaattctg agaccaaggt ggctgaagga
      721 gattatgttt taatcgctac cggaagagta ccaatgactg accatcttta tccagagaaa
      781 gccggaataa agattgatcg aaaagcgatc actactgatt tgcaaatgaa aacttcggta
      841 ccatcaattt ttgctgctgg agatgttaat gggaaatatc ttttagccca tgttgctttt
      901 aaagaagcag aagtagctat taataatatc atgggaaaag aagcaaccat gaattaccga
      961 gtcgtaccaa attgtatttt ttgttcacca gaaatcagct cagtaggttt aaccgaggat
     1021 gaagctatta catcagggtt cgatgtgaaa gttgggaagt ttcctttccg ggcagttggg
     1081 aaagccttga ttgaaggaga aaccgaagga tttactaaaa ttattgctga ccaaaaaact
     1141 ggtgagatat tgggtgctca tataattggg cctcatgcat cggatctgat tggtgagtta
     1201 accttggcaa tggcactgga atcaaccccc gaagagattg cccataccat tcatcctcat
     1261 cctactctca ctgaaaccgt tatggaggca gccgattcag tattcggatc ttcattacat
     1321 ctattttaaa cagttatttg gactgatcat ctggccaaaa cgaggaggtt tcatatgaat
     1381 attcccaaag atttattgta tactcccgag catttgtggg taaaagagga aaatggtgtt
     1441 tatcgctgtg gcatcactga ttttgctcag gaagaattag gagatcttgt gtatgttgag
     1501 ctaccgaagc caaacctaac ggttaaaaaa ggagataaaa tcggtgatgt tgaatcgatt
     1561 aaaaccgtat ccaaccttta ttcaccaatt tctggtgtca taactgaagt gaattctctc
     1621 cttgaaaata aaccagaggt cattaatcaa agtccttatg aaaaaggatg gatttgtgtt
     1681 attcaatcta ctcatccgga agaaattggg caattgctaa gttccgatgg ttataataag
     1741 gttattc
//
LOCUS       DAOSOL010000307         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_68682_length_1742_cov_3.660937, whole genome shotgun sequence.
ACCESSION   DAOSOL010000307 DAOSOL010000000
VERSION     DAOSOL010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68682_length_1742_cov_3.660937"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK171_06515"
     CDS             <1..411
                     /locus_tag="PK171_06515"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HOT05874.1"
                     /translation="AIRNIPGINKTKTREYIKHAVFGIKATLNNAGDKMMDSLYSALE
                     NIFELKQKARVTFKCMAPNASQVYLVGSFNDWNPTVTPLKKTLKGHWSVTLTIPQGSY
                     EYRYLVDNQWFTDPNTPHIFNQFGTENSVLVVGE"
     gene            complement(493..>1742)
                     /locus_tag="PK171_06520"
     CDS             complement(493..>1742)
                     /locus_tag="PK171_06520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT05875.1"
                     /translation="GLNSVISSVTLESIQRGYEVVGIYNGFKWISSASFDPKVHTITL
                     DRKVVDGIHFKGGSILGTARDSLIKNGKIDTEKVNNVKKAMETMDIGYLVTTGGDDTA
                     FSASIISKEIKNFYVAHVPKTIDNDLPLPGDMPTFGFQTARELGTALLKNLLEDARTT
                     GRWYFIIAMGRSAGHLALGMGIAGGATLTIIPEQFGHGKTTLKSVCDIIEGSMLKRKL
                     QGMDWGIGVIAEGVAYKFGDFEQLEKVMGRPIPKDPHGHPRLAEVPLGDLLKREIENR
                     YAEREQKITIVTKDIGYELRCADPIPFDIEYTRELGYGAMDFLLGDDYNADFKEAGAM
                     ISLVEGNLNPIPFHQIMDPDSGKKTIRLVNTYSYNYKVARAFMTLLEPKELNSPDQLS
                     KLSSIAKMDENTFKKHFSNLPRI"
BASE COUNT          533 a          371 c          290 g          548 t
ORIGIN      
        1 gctattcgaa acattccagg gataaataaa accaaaactc gagaatatat caaacacgcg
       61 gtttttggaa tcaaagcaac actaaacaat gctggggata aaatgatgga ttcgctgtat
      121 agtgctcttg aaaacatatt tgagctcaaa caaaaagcca gagttacgtt taaatgtatg
      181 gcaccaaatg cttcacaggt ttacttagtt ggatctttta atgattggaa tcctaccgtc
      241 actcctctca aaaaaaccct taaagggcac tggagcgtta ccctcaccat ccctcaagga
      301 agttatgaat accgttattt agttgataat cagtggttca ctgacccgaa taccccccac
      361 atttttaacc aatttggtac cgaaaattct gttctcgtcg taggagaata attaatcttt
      421 attcataatt ttagttccac ttaccaactc tcctataaaa tttaaaagga aagctggtaa
      481 gtggattatc ggttaaattc tcggtaaatt cgaaaagtgc tttttaaaag tgttttcatc
      541 cattttggca atactggaaa gtttagatag ctgatccggg ctatttaatt ctttaggttc
      601 gagtaaggtc atgaaagctc tggcaacctt ataattgtag gaataggtat ttactaaacg
      661 aatggttttc tttccactat cgggatccat aatttgatgg aaaggaatag ggttaagatt
      721 cccctctacc aaactgatca tagctccagc ttctttaaag tcagcattat aatcatcgcc
      781 aaggaggaaa tccattgctc catatcccaa ctcacgagta tattcaatat caaaggggat
      841 tggatcagca catcttagtt catatccgat gtctttggtt actatagtta ttttttgttc
      901 tctctccgca taacgattct caatttcccg ttttaaaagg tcacctaagg gcacttcagc
      961 caatcgagga tgtccgtgtg gatctttagg tatgggccta cccataactt tttctaactg
     1021 ttcaaaatca ccaaatttat aagctactcc ttcagctatt acaccaattc cccaatccat
     1081 gccttgaagc tttcttttga gcattgaacc ttcgataata tcacaaacac tttttaaagt
     1141 cgtttttcca tgaccaaact gttcgggaat aatggttaaa gtcgcaccac cggcgattcc
     1201 cattcctaaa gctaaatgtc cagcgctcct ccccatggca ataataaaat accatcgacc
     1261 tgtcgtgcga gcatcttcta acaaattctt taaaagggca gttcctaatt ctcgagcagt
     1321 ttgaaaacca aaagttggca tatcaccagg taatggcaaa tcattatcaa tagttttggg
     1381 aacatgagcg acataaaaat tcttaatctc tttggatata atgctggcac tgaaagccgt
     1441 gtcatctcct cctgtagtga cgaggtatcc gatatccatt gtttccatgg ctttctttac
     1501 attattcact ttttcagtat cgatttttcc attcttaatt agactatcac gggcagttcc
     1561 taatattgat ccacccttaa aatgaattcc atcaacaacc tttcgatcta atgttatcgt
     1621 atgaaccttt ggatcaaaag aagcagaaga aatccattta aacccattat aaatgcctac
     1681 gacttcatag cctctttgaa ttgattcaag cgttactgaa gatataacac tgtttaggcc
     1741 tg
//
LOCUS       DAOSOL010000308         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_68727_length_1742_cov_1.614701, whole genome shotgun sequence.
ACCESSION   DAOSOL010000308 DAOSOL010000000
VERSION     DAOSOL010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68727_length_1742_cov_1.614701"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..789)
                     /locus_tag="PK171_06525"
     CDS             complement(37..789)
                     /locus_tag="PK171_06525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT05876.1"
                     /translation="MKGEIDLSHIISLKNRVAIVTGASRGIGRSIAKTLADKGVHLAL
                     VARSLEKMKTLEKEIQTKGIKMRIFPVDISEPSVPEVVVKETIEEFGRLDFLINNAGI
                     ALSKPLIETTVEEWDRQMMVNARAPFLFSREAIPYLKKSDAPSIVNISSVVGYKGYVL
                     QRAYTASNHALMGMTKVLAQEVHQDGIRVYTISPGGVDTELAWNMRPDLDRSILISPQ
                     EIADLILYLLQHRGNAMIDEINVRRVTNQPWK"
     gene            1518..>1742
                     /locus_tag="PK171_06530"
     CDS             1518..>1742
                     /locus_tag="PK171_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05877.1"
                     /translation="MRKVAIIGAGSIVFCKTLMLDIMATNGLEDTTFALMAPSERNIS
                     KVNDFANRVIRENGLPTEVYTTTNSREALKG"
BASE COUNT          522 a          346 c          360 g          514 t
ORIGIN      
        1 ctcgggtttt agaggtattt ccagaattta accttttcac ttccaaggtt ggttcgtcac
       61 tcgacgcaca ttaatttcat caatcatggc atttccgcgg tgttgcaaga gatagagaat
      121 gagatcggca atttcctgag gggaaatgag aatggatcga tctaaatcgg gcctcatatt
      181 ccaagcgagt tcggtatcaa caccccccgg agagatggta taaacccgaa tgccatcctg
      241 atgaacttct tgggcaagaa ccttggtcat tcccatgagg gcatgatttg acgctgtata
      301 cgctcgctgg agaacgtatc ctttatagcc aactaccgat gaaatattta ctattgaggg
      361 tgcatcagat ttttttaaat atggaatggc ttcgcgggag aataaaaaag gagctcgagc
      421 gttaaccatc atttgtcgat cccactcctc aacggtagtt tcaatcaagg gtttggaaag
      481 agctattcca gcgttattaa ttagaaaatc caatctccca aactcttcaa tagtttcttt
      541 tacgactacc tcgggaacac tcggttcact tatatcaacg ggaaaaatgc gcatttttat
      601 ccccttggtt tggatttcct tttccagagt tttcatcttt tccaaagaac gcgctaccag
      661 ggctaagtga actcctttat cggctaaggt tttggcaatt gacctcccta tccctcgact
      721 ggctcccgtc acaattgcta ctctattttt taaagatatg atatgagaca gatcaatctc
      781 tcctttcatc ttcgctattt cttgcttatt ataccatgct ctttttttac tttatcagga
      841 aagaccccct tgccacatag ctgtatcatt tgaggatgaa aatagtcccc tcaccgatac
      901 ctctctcacc atgggagagg aaaaagaagt aataaagcct ttcccatgaa ggggaaaggg
      961 aaaattagtc cccctttaga aaaggtggtt agggggattt gaatatttta actgctccgt
     1021 cattgcgagg agcgtcctat gcgacgtggc aatctcatcc tttaaagttt ttacaaaaaa
     1081 agaattgaaa aaaatgagat cctcacggct tctgaatacg aagcctcagg atgacgggtg
     1141 tggtgtcaga tgagatcctc acgccctcac caaacgaggg ctcaggatga ctgatgaaag
     1201 gcacacctcc ccgccgcgaa agcgactgta ggaaggaggg aaggtgttca acttcttccc
     1261 ccattgaggg gggattcagg ggggtgtgcc tttctctttt tttcatcatt tttcacttat
     1321 tccattattt tcaagttaaa accattgatt ttaaaaattg agtataataa ataatagttg
     1381 ttatagagat attctttata agggtaaacc gtaaaaaagg aggtgttaaa taaaaaagaa
     1441 taaagaaaca ctctgcaata ttttgttata ttcgtcctca ctaattaaaa agttaaaaag
     1501 ggggaattgg taaaatcatg agaaaagtag caattattgg agctggaagt attgtttttt
     1561 gtaaaacatt aatgctggat ataatggcta ctaatggatt agaagatacc accttcgctc
     1621 taatggctcc aagtgaacgg aatatttcaa aagttaatga ttttgcaaac cgtgtaattc
     1681 gtgagaatgg tttaccaaca gaagtgtaca caactacaaa tagtcgtgag gcattaaaag
     1741 ga
//
LOCUS       DAOSOL010000309         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_69140_length_1735_cov_1.954167, whole genome shotgun sequence.
ACCESSION   DAOSOL010000309 DAOSOL010000000
VERSION     DAOSOL010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69140_length_1735_cov_1.954167"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PK171_06535"
     CDS             <1..605
                     /locus_tag="PK171_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05878.1"
                     /translation="LPSMITTIGTQFFWAGATAVVSGGLGKTLVPAKTNIIYSMLVGR
                     LGGKVPAQMIWTVIFAFVIWLFLNRHRFGAHIYFIGDNPESARVMGINVDRVKMMVFT
                     QMGLFSAFAGILASLEVTYYWPTLGQGYLLKTIAAVYLGGTPVEGGVGTVFGTFIGAI
                     ILGCLEAGIIAIGMTGFWTQLIYGLIVIVSIAMHSILRRR"
     gene            complement(669..1020)
                     /gene="rnpB"
                     /locus_tag="PK171_06540"
     ncRNA           complement(669..1020)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK171_06540"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1428..1691
                     /locus_tag="PK171_06545"
     CDS             1428..1691
                     /locus_tag="PK171_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT05879.1"
                     /translation="MIREETSLTRKGQIQLPAKIRKAIGAEIGDTFLFELTDQKEIRV
                     KFVKKIKLLDLAGTFPKKKVFPGIEKEEELTKKKVALKIAQNE"
BASE COUNT          473 a          349 c          367 g          546 t
ORIGIN      
        1 aacttccttc aatgattaca accattggaa cccagttttt ttgggccgga gccactgcgg
       61 ttgtaagtgg tggattaggg aaaactctcg ttcccgcaaa aacgaatatc atttatagta
      121 tgttagttgg gcggcttggt ggaaaagtcc ctgcccagat gatttggaca gtcatctttg
      181 ctttcgttat ctggttgttt ttaaatcgcc atcgttttgg agcacatatc tatttcattg
      241 gcgacaaccc cgaaagcgct cgagttatgg gaattaacgt tgatcgagta aaaatgatgg
      301 tttttaccca aatgggtcta ttttccgctt ttgcaggaat tctggcgagt ttagaagtga
      361 cctattactg gccgactctt ggtcagggat acctgttaaa aacaatagct gctgtctatt
      421 taggtgggac gccagttgaa ggaggggtgg ggaccgtttt tggtaccttt attggcgcaa
      481 ttattttagg gtgcttagaa gcgggaatca tagctattgg tatgactgga ttctggaccc
      541 agctgatata tggcttaatt gtcatcgttt caattgctat gcactcgata ttgcggagac
      601 gataatttgc tggaagtttt aaaaaaaaag agggattaaa ttatccatct gataacttaa
      661 tccctctttt tttgggaaga tcgataagcc gaattttgta ttctcaaaag agaacggcaa
      721 ccatctatct gggaaaccgg ttacccggtt cctcaagcag ccaacccgaa ggtcagcgag
      781 cagcctcatt ccttcctatt tggccttgct ccaggtaggg ttttctagat acaccggtta
      841 cccggtgatc ggtgagctct tacctcgcct tttcaccctt accttggtaa aaaaaccaag
      901 gtggtttgct tttctgtggc actttcccga ggttaccctc gctggacgtt atccagtacc
      961 ttgctctgtg gagttcggac tttcctcagg tttttcgcct gcggttgcct gaccttccct
     1021 ttattattat acactatcct gaaaataccg tacttatttt gatgataact cattgagagc
     1081 agcaaactag aatctcattc tgggttctca ccctcatctt caccttctct catcaaggga
     1141 gaaggaacta taacgataca tcatcctgag cggtgctttt ctgcgtgagg atctcatctg
     1201 acgccgcaga cgtcatcctg aggcttcgct ttttgaagcc gtgaggatct catcttttta
     1261 ttttttttct ttaattcgcc cctttacgcc ttcataaagc aaaagctcgg gatggagtca
     1321 ttttttactt gtttctttat ttcctttatc gtatcatttt gtcatcgttt cattgaatga
     1381 taaaaatttc aaagatataa aataaatcag aaaagttggt aatatcaatg attagagagg
     1441 aaaccagctt aaccagaaaa ggacaaatcc aacttcctgc caaaatccgt aaagctattg
     1501 gagctgaaat tggggatact ttcctttttg agcttactga tcagaaggaa ataagggtta
     1561 aatttgtaaa aaagataaaa ttactcgatt tagctgggac ttttcccaag aaaaaggttt
     1621 ttcccgggat agagaaagaa gaagaactaa ccaaaaagaa agtagcactt aagattgctc
     1681 agaatgaata aaaagatgaa aaaatcagct tggcttgata ccaatataat tcttc
//
LOCUS       DAOSOL010000310         1730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_69389_length_1730_cov_2.920000, whole genome shotgun sequence.
ACCESSION   DAOSOL010000310 DAOSOL010000000
VERSION     DAOSOL010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69389_length_1730_cov_2.920000"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PK171_06550"
     CDS             <1..1493
                     /locus_tag="PK171_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOT05880.1"
                     /translation="LRRNIQNKEYRQKGKIIQLNQVVQKYYYDSLLHSEDPLARTSRQ
                     YLFQKRGIKPSSAEQFGLGYSPADGKAIIEYLYQQGFNGNDFIQAGIGNVIKKGELYD
                     RFRGRITFALHDSNGEIVGFAGRTLLENENPKYINSSESPVFSKGNNLYGLFATKSDI
                     RKTKTVILVEGYMDFLSLFENQITNCVASMGTALTAHQATLIRRFAEEVVICYDSDNA
                     GRMATFRGIDILTKKGLSVKIAVLPSPYDPDTYIRKKGREAFLEILEQKKPIFDYQLE
                     LLLNEYGSKSLESQIHIIRGLLPSINAMTDSIERSLKIRSLAQYLKIPESLIYNVLKG
                     TRESEKKVKLFKTAQKDAIHGAIKAEKILVKFLLEEPTVRSMICDQVPAEYFSVPEHR
                     RIYQAILSNLGEEFSLSDLINVFSGDESMSSCISALGSTETECYEMNEELIQSLIKTL
                     KRAELRRERSILASELAQQADYESKLLIGKRIEEIDKEIQGLKTCS"
BASE COUNT          579 a          299 c          360 g          492 t
ORIGIN      
        1 ccttaaggcg gaatatacag aacaaggaat atcgacaaaa agggaaaata atacagttaa
       61 accaagttgt gcaaaaatat tattatgatt cactccttca ttctgaagat ccattggcac
      121 gaacatcaag gcaatatctc tttcaaaaaa ggggcatcaa accgtcgtca gctgaacaat
      181 tcggacttgg ttattcacca gcagatggaa aggcaattat cgagtatttg tatcaacagg
      241 gatttaatgg gaacgatttt atccaggcag ggataggaaa tgtcattaaa aagggagaat
      301 tgtatgatcg attccgagga cgaataactt ttgcgcttca cgacagcaat ggagagattg
      361 taggctttgc tggaagaacc cttcttgaaa atgagaatcc taaatatatc aatagttctg
      421 aaagcccggt tttttctaaa ggaaacaatt tatatggttt atttgccacg aaaagcgaca
      481 tacgaaaaac taagacggtc atattagttg aaggatacat ggatttccta tcccttttcg
      541 agaaccagat aaccaattgc gtggcatcga tggggacagc cttaacggct catcaagcaa
      601 ctcttatcag gaggttcgct gaagaggttg ttatctgcta tgattcagat aatgccggaa
      661 gaatggcaac tttccgggga atagatattt taaccaaaaa gggattatcg gttaaaatag
      721 cagtgttacc atctccctat gatcctgata cctacattcg caagaaagga agagaagcgt
      781 ttttagaaat tctggaacaa aaaaaaccaa tttttgatta tcaattggaa ttattgttga
      841 atgaatatgg ttctaaatca ttagaatcac aaattcatat cattcgcggt cttcttcctt
      901 ccatcaatgc tatgactgat tccattgaaa ggtctttaaa aatacgttct ttggctcagt
      961 acctaaaaat tcctgaatca ttaatctata atgttttaaa gggaactcgg gagagcgaga
     1021 aaaaagtaaa actctttaaa acagcccaaa aagatgctat tcacggagca ataaaggccg
     1081 aaaaaatact ggtaaaattt cttttagaag agccaaccgt cagaagtatg atttgtgacc
     1141 aggtaccagc tgagtatttt tctgttccag aacaccggag aatatatcag gcgattttaa
     1201 gtaatttggg tgaggagttt tctctatctg acctgattaa tgtattttct ggagatgagt
     1261 cgatgtcgtc ttgtatttca gctttgggtt ctacggaaac cgaatgttac gagatgaatg
     1321 aagagttaat tcaaagcctg atcaaaactc taaaaagagc ggaattaaga agagaacgat
     1381 caatactggc tagtgaatta gctcagcaag ccgattatga atccaaactt ttgattggaa
     1441 agcggataga agaaatagat aaagaaatac aagggttaaa aacctgttct tgattttttt
     1501 atttaaaaga aggagataag cgtttatgcc acggttgtgg caccagatga ggatgaaaat
     1561 acttatataa aaccgtcatt gattgccaag agtccaacca ttctttggtt ggtcgacgta
     1621 gctattttat caatccattc cgtcattctg aggattccgg tgtcttttac cgggtgacgt
     1681 ggcaatctca tccacccact ccgtcattct gaggagcgta ttatgcgacg
//
LOCUS       DAOSOL010000311         1723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_69791_length_1723_cov_3.946643, whole genome shotgun sequence.
ACCESSION   DAOSOL010000311 DAOSOL010000000
VERSION     DAOSOL010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69791_length_1723_cov_3.946643"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /gene="lon"
                     /locus_tag="PK171_06555"
     CDS             complement(<1..1696)
                     /gene="lon"
                     /locus_tag="PK171_06555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT05881.1"
                     /translation="MAKTQREYFLREQLKAIQRELGEMDEKTIELNELKEKIEKAKLP
                     PEVLKEAEKELERLSRIPPVSAEYPVIRNYLDWITELPWNIRTEDSLDLKKAEKVLNE
                     DHYDLEKVKERILEYLAVGHLKKDLKGPILCFVGPPGVGKTSLGKSIARALGRKFVRI
                     SLGGVRDEAEIRGHRRTYVGALPGRIIQGIRKAGSNNPVFMLDEVDKIGLDFRGDPSA
                     ALLEVLDPEQNSTFVDHYLAVPFDLSKVIFIATANILDTIPPALLDRMELIRIPGYTD
                     PEKIAIARKYLIPKQSKENGIEDQTVTISDEILIKMIREYTREAGVRNLERTIATVCR
                     KIAKDVASGKKGPFVVDEENLRKYLGKRIYFDEVMSGKDRVGVATGLAWTQTGGEILF
                     VESSVLSGKGNLILTGNMGDIMQESARIALSCVRERAKKWKIDEKFYEKHDIHVHVPA
                     GAIPKDGPSAGITLVVSMVSSLSQIPVKHSIAMTGEITLTGKVLPIGGVKEKSLAAYR
                     SGIKEVVLPQENENDLDDIPSDVRKKMKFHLVKTIDDVLKITLKKSPRMVKKKKTSS"
BASE COUNT          462 a          374 c          284 g          603 t
ORIGIN      
        1 gagaagaagt ttttttcttt ttaaccattc ggggtgattt ttttaaggta attttcagaa
       61 catcatcgat agttttgact aaatgaaatt tcattttttt tctcacatcg ctcggtatat
      121 cgtctaaatc attttcattt tcttgaggta gaacaacttc cttaattccc gaacgatagg
      181 ccgctaagga tttttctttc actccaccaa tgggaagcac ttttccagtt agggttattt
      241 ctccggtcat tgcaatcgaa tgttttacag gtatctgcga aagagaagag accattgaaa
      301 caaccaaagt tattcctgca gatgggccat cttttggtat agcaccagca gggacatgaa
      361 cgtgaatatc atgtttttca taaaattttt catcaatttt ccactttttc gcccgttctc
      421 gaacacaaga aagggcaatt cgtgccgact cttgcataat atctcccata ttaccagtta
      481 atatcaagtt tccttttcct gagaggaccg aagactcaac aaaaaggatt tctccaccag
      541 tttgagtcca agctaaaccg gtggcaactc caactcgatc ctttcccgac atgacctcat
      601 caaaataaat tcttttacct aaatattttc taagattttc ttcgtcaact acaaatggtc
      661 cttttttccc tgaagcaacg tctttagcaa ttttcctgca aaccgtggcg attgttcttt
      721 ctaaatttct gactcccgct tcacgagtgt attctcgaat cattttaatt aaaatttcat
      781 cacttatcgt aaccgtttga tcctcgattc cattttcttt gctctgcttg ggtattaaat
      841 atttgcgagc aatggcaatt ttttccggat cagtataacc agggattcgg atgagttcca
      901 ttcgatccaa caaagctgga ggaatggtat ccagaatatt cgcagttgcg ataaaaataa
      961 ccttcgatag gtcaaatgga actgctaaat aatgatcaac aaaagttgaa ttttgctcag
     1021 gatctaaaac ttcgaggaga gcagcagaag gatctcccct gaaatctaaa ccgattttat
     1081 caacctcgtc taacataaat accgggttat tgcttcctgc ttttcggatt ccctgaataa
     1141 tccgacccgg aagtgctcca acgtaggttc ggcgatggcc tcttatttcc gcttcgtctc
     1201 gtactccacc taaggatatc cgtacgaatt ttcttcccaa tgcccgggca atcgattttc
     1261 ctaatgaagt ttttccaact cctgggggac caacaaaaca aagtattggt ccttttaaat
     1321 cttttttcaa atgacctact gccaagtatt caagaattct ttcttttact ttttccaagt
     1381 catagtgatc ttcatttaat accttttcag cttttttaag atctaaactg tcttcggttc
     1441 ttatgttcca aggaagttcg gttatccaat caagatagtt acgaatgact ggatattcag
     1501 ctgaaactgg tggaattctc gataatcgtt ccagttcttt ctcggcttct ttcaaaactt
     1561 ctggcggaag tttggctttt tcaatttttt ctttcaattc atttaattca atggtttttt
     1621 catccatttc tccaagctca cgttgaattg ctttcaactg ctctcttaag aaatattctc
     1681 tttgagtttt tgccatctct gattgaattt gagtctgaat ctt
//
LOCUS       DAOSOL010000312         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_69925_length_1720_cov_4.438438, whole genome shotgun sequence.
ACCESSION   DAOSOL010000312 DAOSOL010000000
VERSION     DAOSOL010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69925_length_1720_cov_4.438438"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            687..>1720
                     /locus_tag="PK171_06560"
     CDS             687..>1720
                     /locus_tag="PK171_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT05882.1"
                     /translation="MMKFKPTVILLISLFLIVLTVQVGFAQQLEPPPAEPSQWTGLIG
                     TYSLSSQDGNTFYLRENQDRLEILFPLADTHRKTESLVFQGKTFEIIPLKEIDSDTFQ
                     LPPVNELGARKFRILRDQDGFGVTIDAGKKEYQRLFYGPERGKVFKIQPMKSLEELQA
                     EAIKANPPTEKGEFLKVDLVELVKLDKNLKLDIRYATENNFMGIPLYSQPRAFLQRPV
                     AEALVKAHKKLQAIGYGLIIFDAYRPWYVTKMFWDATPLSMRNFVADPKSGSRHNRGG
                     AVDVGLYDIESKQPIFFGGDFDEFSERSFVSYPGGTSRERWFREILRQALEAEGFEVY
                     EDEWWHFDYK"
BASE COUNT          522 a          316 c          374 g          508 t
ORIGIN      
        1 tttgctattc taaaaaccta tttgcaggca tgataaatca agcccttata aaagataata
       61 aaaatgtagg ggcgcaatgt aatttaccca attttcattt atcatagcga tatgccaggg
      121 catgtcgaat cttggttttc atccttatct ggtgttgcca tgtggcatga aagtctatcc
      181 tggaaattcc ggaataagtg aaaagtgatc aaaacaaaaa gataaagaat ggattcaaca
      241 attcccctcc ttggaggggt gccgttttcc ggcggggagg gtgcttttaa ttcgtcatct
      301 taaggcttcg tactttgaag ccgtgaggat ctgatctttt atattttaat tttggaaaag
      361 atttaaaagg atgagattct cacgtcgtcc ggaaaaagac accggacttc tcagaatgac
      421 aaactgggtg acagagattg ccacgtcact acgttcctcg caatgacgga acagtaaaaa
      481 ccaaatcccc ctaaccccct tttctaaagg gggagattgg ttcttgagta tatattttca
      541 ttcccatctg gtgccacaac gtggcatgaa catctatatc tttcctgtaa ttacagaaac
      601 gagtgaatag taatcaaaca aaaatgagta gggtacgccc cattgaatcc cctcaatggg
      661 ttaattaaaa gaaagaaagg atgttgatga tgaaatttaa accaacggtt attcttttga
      721 taagtctttt tctcattgta cttaccgttc aagttggttt tgctcaacag cttgagccac
      781 cccccgctga accatcccaa tggacgggac taattgggac ttattctcta tcttctcagg
      841 atgggaatac cttttatcta cgagaaaacc aagatagatt ggaaatcttg tttcctttgg
      901 ctgatactca tcgaaaaact gagagtctgg tttttcaagg aaaaaccttc gaaattattc
      961 ctttaaaaga aattgattcc gatacctttc aattgccacc cgtgaatgaa ttgggagcga
     1021 gaaagtttcg cattcttcga gatcaggatg gttttggagt aactattgat gccggaaaaa
     1081 aagaatatca aagactattt tacggtccgg agagggggaa ggtttttaaa attcaaccga
     1141 tgaaatcatt ggaagaactt caagccgaag cgataaaggc gaatcctcca accgaaaaag
     1201 gtgaattttt aaaagtggat ttagtcgaat tagttaagct tgataaaaac ctaaaacttg
     1261 atatccgcta tgcaactgag aacaatttta tgggaatccc tttgtattct cagccgcgag
     1321 cttttttaca gcgaccagtt gccgaagctt tggtgaaagc ccataaaaaa cttcaagcaa
     1381 tcggttatgg tttgatcatt tttgacgctt atcgtccctg gtatgtaacc aagatgtttt
     1441 gggacgcaac cccactttca atgagaaatt tcgttgctga tccgaaatcc ggatctcgcc
     1501 ataatcgagg tggtgcagtt gatgttggct tatacgatat tgaaagcaag cagccgatat
     1561 tttttggcgg tgatttcgat gaattttccg agcgatcttt tgtctcttat ccggggggaa
     1621 ccagtcgaga gagatggttt agggaaatat tgcggcaagc tttagaggca gaaggatttg
     1681 aagtgtatga agatgaatgg tggcattttg attataaaga
//
LOCUS       DAOSOL010000313         1717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_70083_length_1717_cov_5.234657, whole genome shotgun sequence.
ACCESSION   DAOSOL010000313 DAOSOL010000000
VERSION     DAOSOL010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70083_length_1717_cov_5.234657"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..846)
                     /locus_tag="PK171_06565"
     CDS             complement(37..846)
                     /locus_tag="PK171_06565"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOT05883.1"
                     /translation="MKDYLLELASQQTNSYNKLNVMREYLQAYTIRILFEEGAFRTLA
                     FIGDTALRFVYHLPRYSEDLDFSILKSSDRYSFLTLLNRLKKEFILAGYEIDISYNDQ
                     KTVQSAFLKFPKLQLEAGISSFPDQNISIKIEIDTNPPSSAQIQSHIINKYFPLSFTS
                     YDVPSLFAGKIHALLTRKYTKGRDYFDLGWYLSRWKEITPNFTLLINALKQTHYSGNY
                     PTPENWRQILIRTIQKTNWKTVTDDFQNFLENPSELTIFTEDNLLKLLKKP"
     gene            complement(843..1481)
                     /locus_tag="PK171_06570"
     CDS             complement(843..1481)
                     /locus_tag="PK171_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05884.1"
                     /translation="MKWEEFLKQVNHLPVIESEMLLTGAADPAALSVQLVRWAKAGKI
                     IQVKRGVYLLSEIYRKIDVYEPHLASILIKPSYISLEKALEFHGLIPEGVPVYTSVTT
                     KRSGRFITPVGVFDYRHLQPSLFWGYQSITLRQQTAFIAQPEKALLDFFYLKKIKVSH
                     DFLEEMRLQNCEIISNQTLIAYAQRFQKPGILAAAKTLQQYLITQKGYVKLQ"
BASE COUNT          535 a          272 c          385 g          525 t
ORIGIN      
        1 aaactatacc tcaatattat cgggaaagac ctaaaattaa ggttttttaa gaagtttgag
       61 gagattatcc tcggtaaaga tagtaagttc agaaggattt tcgaggaagt tttggaaatc
      121 atcagtaaca gttttccagt ttgttttttg aatggttctt attagaattt gacgccaatt
      181 ttcaggagtg ggatagtttc cagaataatg agtttgtttc aaagcattaa taagcaaagt
      241 aaaattggga gtaatttctt tccatcgaga aagataccat cccagatcaa agtaatcacg
      301 accttttgta tattttctgg ttaaaagagc atggattttt ccggcaaata gggaaggaac
      361 atcataactg gtgaaagaaa gtggaaaata tttattgatt atatgggatt gaatttgagc
      421 actcgatggg ggattggtat caatttcaat cttaattgag atattttggt ctggaaatga
      481 tgaaatcccc gcttcaagtt gaagcttggg gaacttgagg aaagcagatt gcaccgtttt
      541 ttgatcgtta taggaaatgt ctatttcata accggcaaga ataaattctt tttttagccg
      601 atttaataag gtgaggaatg aatacctgtc tgaagatttt aaaattgaaa aatccaaatc
      661 ttcggaataa cgggggaggt gataaacgaa tcgtaaggct gtgtcaccaa taaatgcaag
      721 agtgcggaaa gcaccttctt cgaagagaat acgaattgta taggcttgaa gatattcccg
      781 catcacattc agcttattgt atgagttggt ttgctgggat gccagttcta ataagtagtc
      841 tttcattgga gttttacata acccttttgg gtaatcagat actgctggag ggttttggca
      901 gctgcaagaa tgccgggttt ttggaaacgt tgagcatagg cgatgagagt ctgattgctt
      961 atgatttcac aattttgaag acgcatttct tccaggaagt cgtgagagac tttaatcttt
     1021 ttgagataaa aaaagtcgag gagagctttt tctggctggg caataaaggc ggtctgttga
     1081 cggagagtaa tggactgata tccccaaaaa agggagggct gtaaatgacg ataatcgaat
     1141 accccaaccg gagtgataaa ccgtccggat cgtttggtcg ttaccgaagt ataaactggc
     1201 accccctctg gaataagccc atgaaattct aatgcctttt caaggctgat gtatgatggt
     1261 ttgataagaa tcgatgccag atgaggttca tagacatcaa tttttcgata aatctcgctg
     1321 agaaggtaga ctcctctttt cacctgaatg atttttcccg cctttgccca ccgaactaac
     1381 tgcactgata aggcggcagg atcagccgca ccggtaagaa gcatttctga ttcaatcacc
     1441 ggtagatgat taacttgttt taaaaactct tcccatttca tatcaacata ttatcattta
     1501 tgttaagtta ttacaacaaa taatgataaa aataatttaa ttagtcggaa tttgaaattg
     1561 gttggaaaaa aagagaacta agttttttat tcttcaaaaa tattttaaag gatgggattc
     1621 tcacgtcgca caattcgctc ctcagaatga cggagtgggt ggatgggatt ctcacgtcgc
     1681 acaattcgct cctcagaatg acggagtggg tggatga
//
LOCUS       DAOSOL010000314         1705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_70757_length_1705_cov_4.084848, whole genome shotgun sequence.
ACCESSION   DAOSOL010000314 DAOSOL010000000
VERSION     DAOSOL010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70757_length_1705_cov_4.084848"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..736)
                     /locus_tag="PK171_06575"
     CDS             complement(59..736)
                     /locus_tag="PK171_06575"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT05885.1"
                     /translation="MGDQTETKQLNNKNHRITSIIAAYNEEKTIGPIIDVLKSVPIIE
                     QIVVVSDGSTDRTVDIARSRNVEVVDLISNVGKGGALYRGLEYIRTDVVLLLDADLVG
                     LEESHVLSLVHPVTEREADVTIGVFEEGRFATDFAQKIAPLLSGQRAIRTDVIQNISD
                     LEVSRYGVEVAMNRYIRKNGVTMKLVKLPGLTHVMKEEKMGLMKGLRHRVKMYWEILK
                     NSYGGGG"
     gene            complement(739..>1705)
                     /locus_tag="PK171_06580"
     CDS             complement(739..>1705)
                     /locus_tag="PK171_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HOT05886.1"
                     /translation="RRERDFCLGPTHEEVITDIARKEIRSYRQLPLLLYQIQTKFRDE
                     IRPRFGVMRSREFIMKDLYSFDRDYEGLQVSYQKMYNAYSKIFSSFGLEYIAARADSG
                     VIGGDISHEFLIIASSGEEKVMRCPKCLATSTQEVNDCPKCHADMEEKRGIEVGHIFQ
                     LGTKYSESMKAYFVDQDGKEKPLIMGCYGIGIGRTMAAAIEANHDENGIKWPWNIAPY
                     EIIVIPVKLKNQASIELAKSISQQLVYEEYEAIWDDRDVSAGYKFKEADLIGFPIQVI
                     VGEKALKNSILEIKIRSNGKRIECTREQLISVIKKIKEELVILQE"
BASE COUNT          486 a          405 c          252 g          562 t
ORIGIN      
        1 accactgcaa acaataccaa gggttttaat gaataatttt tgattattca gagctatatc
       61 aacctcctcc tccataagaa ttttttaaga tttcccaata cattttaact cgatgacgaa
      121 gtcctttcat caagcccatt ttttcttcct tcatcacatg agtcaacccc ggtaacttta
      181 ccagtttcat cgtcacaccg ttttttcgaa tataacggtt catagcaact tcaaccccat
      241 atcgggatac ttccagatcg gatatgtttt ggatgacatc ggtccgaatc gcccgttgtc
      301 cagataataa tggagcaatt ttctgggcaa aatcggtcgc aaagcggcct tcttcaaata
      361 ccccaatggt tacgtcggct tctctctcgg taacaggatg aaccaaactc aaaacatgac
      421 tttcttctaa accaaccaaa tccgcatcta ataggagaac aacatcggtt cttatatact
      481 ctagccctcg ataaagggca ccaccctttc ctacattact aattaaatct actacctcaa
      541 cattacgact tcgtgcaata tcaactgttc gatcggttga cccatcgctc actactacaa
      601 tctgttcaat aatgggtaca cttttcaaaa catcaataat tggtcctatc gttttttctt
      661 cattataagc agcaataatt gacgtaattc ggtggttttt attatttaat tgttttgttt
      721 cagtttgatc tcccatgctc attcctgcaa aataaccaat tcttccttaa ttttttttat
      781 cactgatatc aattgctccc gggtacactc tatcctcttc ccgtttgaac gtattttaat
      841 ttccaaaata ctatttttca aggctttttc accaacaatt acttgaatag ggaatccaat
      901 taaatcggct tctttaaatt tatatccagc ggaaacatca cgatcatccc atatcgcctc
      961 atactcctca taaaccagtt gctgggaaat acttttggct aactctatac tggcttgatt
     1021 ttttaatttt accggtatga caattatctc ataaggagcg atattccagg gccattttat
     1081 tccgttttca tcgtggttgg cttcaattgc tgcagccata gttcttccaa tgccaattcc
     1141 ataacatccc ataatcaaag gtttttcttt tccgtcctga tcaacaaaat aggctttcat
     1201 cgattctgaa tatttggttc ccaattgaaa aatgtggcca acttcaatgc ctcgtttttc
     1261 ttccatatca gcatggcatt tcggacaatc gttgacttcc tgggttgagg tggccaaaca
     1321 ctttggacat ctcatcactt tttcttctcc agagctggca ataatgagaa attcatgaga
     1381 aatatcacca ccgattactc ccgaatcggc tctcgctgca atatattcta aaccaaaaga
     1441 tgaaaatatc tttgaatagg cattatacat tttttgataa ctaacctgta atccttcata
     1501 atcccgatcg aagctataca aatccttcat aatgaattct cgagatcgca ttactccaaa
     1561 gcgcggtcgg atttcatctc gaaatttagt ttgaatttga tacaacagca aaggaagctg
     1621 tcgataagaa cggatctctt tcctggctat atcggtaata acctcttcat gggttggtcc
     1681 caaacaaaaa tccctttctc ggcgg
//
LOCUS       DAOSOL010000315         1679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_72308_length_1679_cov_4.458128, whole genome shotgun sequence.
ACCESSION   DAOSOL010000315 DAOSOL010000000
VERSION     DAOSOL010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72308_length_1679_cov_4.458128"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="PK171_06585"
     CDS             complement(<1..1013)
                     /locus_tag="PK171_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOT05887.1"
                     /translation="MTTEELYQSRLKRYVTAMRNGKPDMIPIRPFVAEFASQYAGYTA
                     QQATHDYRYAVKAYLKCAHDFDWDAIPANMIYCWTGLVQAIGLTYYGIPGIDLPPQVG
                     FQYKEPPEEKAFMPPEDYDLLIADPTGYLFNIWLPQVSSEIQRPGQPISERHNLALLK
                     GGMAMTEYFNVLGNLAQSLQTECGTVSAICGILKAPLDILADKLRGYYGLANDLCYQP
                     KKVLEACEAMMPHLLQVALDSADPKKQVPIGFWMHRSCVPLISHDHFQNIFWPTLKPI
                     IEEIWKHGNQVLFYAEGNWDHHLETFRELPAGSIIYHVDRGDIFLAHQIFGDKFCLSG
                     GIP"
     gene            complement(1447..>1679)
                     /locus_tag="PK171_06590"
     CDS             complement(1447..>1679)
                     /locus_tag="PK171_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05888.1"
                     /translation="FYSEKYINKNYRMDFALDLGLKDLNYMKKLCDDLNIPGFLLDGA
                     IDLCRVALKEGKGKELDFSHAGNTMYEYVGLE"
BASE COUNT          526 a          325 c          340 g          488 t
ORIGIN      
        1 ttgggaattc cgccacttaa acaaaactta tcaccgaaaa tctgatgagc taagaaaata
       61 tctccccggt caacatggta gataatgcta ccggcaggta gttcccgaaa cgtttccaaa
      121 tgatgatccc aatttccttc agcgtaaaag agaacttgat ttccgtgttt ccatatttct
      181 tcgataatag gtttgagagt aggccaaaag atattttgaa aatgatcatg agaaatgaga
      241 ggaacacaac tgcgatgcat ccagaaacca ataggtactt gtttttttgg atcagcgcta
      301 tcaagagcga cctgcagaag gtgaggcatc atcgcttcac aagcttctaa tacctttttt
      361 ggttgatagc agaggtcatt agctagtccg taatatcccc ttaatttgtc agcaagaata
      421 tcaagaggag ccttgagaat accgcagatt gctgacacgg ttccgcattc ggtttgcaga
      481 gattgggcca aatttcctaa aacgttaaaa tattcggtca tggccatgcc gcccttaagt
      541 agggcaagat tatgacgctc actaatcggt tggccagggc gttggatttc gcttgaaact
      601 tgaggcagcc aaatattaaa taagtatcca gtagggtcag ctattaataa atcatagtcc
      661 tcgggaggca tgaaagcttt ttcttcgggg ggttctttat actgaaagcc aacttggggc
      721 ggtaaatcaa tcccaggaat accataataa gtaaggccta tcgcctgtac gaggccagtc
      781 caacagtaga tcatattcgc aggaatggca tcccaatcaa aatcatgagc acatttcaaa
      841 taagctttta ctgcatagcg ataatcatga gttgcttgtt gagcggtata accagcatac
      901 tgagaagcaa actcagcaac aaaaggacgg atagggatca tgtctggttt gccgtttctc
      961 atggcggtga cataccgttt aagccgggat tgataaagtt cttcggtggt catcaagaat
     1021 cctccttgta aagtagatcg tgttccttca ttttatcatt cttcataata ctatcaaaaa
     1081 acaatagaat actttaattg gtaaattaat tttctctcac ttttccgaaa gagaaattga
     1141 gagggattcg atttttttct gaatttttaa atctccctct catccccctt ttccaagagg
     1201 aggatcaatt gctgagcaag gattttcatc ctcttatgtt ccacaaggtg gcattagggc
     1261 ttattatata aattccaaga tgtaggccaa ggggaaaaaa gaaggcgatt aaattaggaa
     1321 agaaaagggg aaatagaaat ttaatataaa aaaaagagaa tataatcaca acccaaccga
     1381 tgtcgactaa aaaggtgtaa atcaattttt ttaagttgag gtcgacatcg gttgggttct
     1441 atagatttat tctaatccta cgtactcgta catagtattt ccggcatggc tgaaatctag
     1501 ctccttgccc tttccttctt tgagggcaac tcggcagagg tcaatagcac catctaataa
     1561 aaatccagga atattcaggt catcgcacag ttttttcata taatttaagt ctttaagtcc
     1621 gagatcaaga gcaaagtcca tgcgataatt cttatttata tatttttcgc tataaaact
//
LOCUS       DAOSOL010000316         1679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_72333_length_1679_cov_2.181650, whole genome shotgun sequence.
ACCESSION   DAOSOL010000316 DAOSOL010000000
VERSION     DAOSOL010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72333_length_1679_cov_2.181650"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PK171_06595"
     CDS             <1..411
                     /locus_tag="PK171_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05889.1"
                     /translation="WYHLFPPTGLATIRRKPWLSEKESAWYSGDVSKPDQFLILARGE
                     NVATFLQMFIETETEDSEIVEDISKSIGNAEFRMVTMESPQDDETIIFLAADKVFNNG
                     DGLFLNITIFGSASEEFQPILEKILDSFSFTVEE"
     gene            683..>1679
                     /locus_tag="PK171_06600"
     CDS             683..>1679
                     /locus_tag="PK171_06600"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT05890.1"
                     /translation="MLSLSNHFIFGFLIFCLILMASSGVYADNNSTPSVNQTTQINLA
                     RSSMKVKGFSDPEMDFQLMRSIGTDWYGGGILGEILLAASEITDGDPSNWPKSFAQLG
                     MQVEKDGRERLAKGHVVSARDALMRASNYYRAAEYYADPRLPEHQEFGLKARTCFIDG
                     INLFDWRVEVLQIPYQDSYLTGYFLSPDKDTNQITRKTILIMSGYDGTSEEIFFGAGR
                     AALERGYNILLFDGPGQVGALRFHPHMAFIPDYSQPITAVVDFALKRKDVDPNQLALY
                     GISLGGYFAASGAITEKRIKALILNSPIVDIHEYLIAGGLSQLENMPEDLRLEDFD"
BASE COUNT          504 a          325 c          342 g          508 t
ORIGIN      
        1 tggtatcact tattccctcc caccggattg gcaacaatta gaagaaaacc ttggcttagc
       61 gaaaaagaat cggcttggta cagcggagac gtaagcaagc ctgatcaatt tttgatttta
      121 gctcgaggag aaaacgtcgc aaccttcctt caaatgttca ttgaaaccga aaccgaagat
      181 agcgaaattg tagaagatat atcaaaatca ataggtaatg cagaatttag aatggttact
      241 atggaaagcc ctcaggatga tgaaacgata attttcttgg ccgctgataa agtattcaac
      301 aatggagatg gtttattttt gaatattact atttttggca gcgcatccga ggaattccag
      361 ccaattctgg aaaaaatctt ggattcattt tctttcactg tcgaagaata ataaattaac
      421 ctaacttaat gagccggtat cccgtgtctt catatactga ggcacgggat tttttctccc
      481 ctccttcttc ccgatccgaa aataccatct ttaaattccc ccattgaggg gggattaagg
      541 ggggtgtgcc tttaatcggt catcctgagc cctcttcctt gttcttctgc agaaaaacta
      601 ataagtttga acttcaaacc gataataaat ataataaaaa attaaaataa gtctttcccg
      661 atcgaggtga aacaccatgc atttgctgag tttatcgaat cactttatct tcggatttct
      721 cattttctgt ttaatcttaa tggcttcttc tggagtatat gcagataata attcaacacc
      781 gagtgttaat caaactaccc agatcaattt agcaagaagc tcgatgaaag ttaaaggctt
      841 ttccgatcca gaaatggatt ttcaactcat gcgctcaatt ggtactgatt ggtatggagg
      901 tggaatttta ggggaaatac ttttagcggc ctcagaaata accgatggtg atcccagtaa
      961 ttggccaaaa agttttgctc aattaggaat gcaagtagaa aaagacggcc gtgagcgttt
     1021 agccaaaggt cacgtggtaa gcgctcggga cgctttaatg agagccagta attattatcg
     1081 agcagctgaa tattatgccg atccccggct tcctgaacac caagaatttg gtttaaaagc
     1141 ccgtacctgt tttatcgatg gaataaattt gtttgattgg agggttgaag ttcttcaaat
     1201 cccttatcag gatagttatc ttaccggtta tttcctttct ccagacaaag ataccaacca
     1261 aattactcga aaaaccattc tgattatgag cggatatgac ggtacctcgg aggaaatatt
     1321 ttttggcgct ggacgggctg ccttagaaag aggatacaat atactgcttt tcgacggacc
     1381 cggtcaagtt ggtgctcttc gttttcatcc tcatatggct tttattccag attatagtca
     1441 gccaataact gcagttgtcg attttgcttt gaaaagaaaa gatgttgatc ctaatcagct
     1501 tgctttatat ggaattagct taggaggcta ttttgctgcc agtggagcaa taactgagaa
     1561 acgcatcaaa gccttaattc tcaattcacc gatagttgac attcatgagt acttgatagc
     1621 cggtggactt tctcaattag aaaatatgcc agaagacctc cgacttgaag actttgatt
//
LOCUS       DAOSOL010000317         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_72360_length_1678_cov_3.725816, whole genome shotgun sequence.
ACCESSION   DAOSOL010000317 DAOSOL010000000
VERSION     DAOSOL010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72360_length_1678_cov_3.725816"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..936
                     /locus_tag="PK171_06605"
     CDS             142..936
                     /locus_tag="PK171_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT05891.1"
                     /translation="MFNSELNKPIIIVEELTKIYKMDSFELKALQNVSFKVYSGEFVS
                     VMGPSGSGKSTLMNLLGCLDTPTSGRYLLDQQDVSKLKDNTLATFRNKKIGFVFQSFN
                     LLPKLDAFQNVELPLIYAGVSRVERYEIVKKFLTKVGLEDRMHHRPNQLSGGQTQRVA
                     IARALVNDPSIILADEPTGNLDTRSGEEIMQIFQTLNDEGKTIILVTHEHDISLHTKR
                     ILSFRDGQLVGDEKVENPVRAEDSLKTYTDQEEKQKLEESQLSTKS"
BASE COUNT          536 a          306 c          334 g          502 t
ORIGIN      
        1 tctcgaaggc ccaactctac cagatagctg ggaaggagat tgttgttgag gaactgtaat
       61 tccctttgca acataaaaca gaatagttat ttagtaatca taaataatcg aatttaaata
      121 tttttataac ggagtttaat aatgtttaat agcgaattga ataaaccgat tattatagtt
      181 gaagagttaa ctaaaattta taagatggat tcatttgaac tgaaagccct tcagaatgtt
      241 tcttttaaag tttattctgg agagtttgta tcggttatgg gtccatctgg ttcaggaaaa
      301 tcaaccttaa tgaatctctt ggggtgcctt gataccccaa ccagtggtcg atacttacta
      361 gatcaacaag atgtatcaaa attaaaggat aatactttgg caacctttcg aaataaaaaa
      421 ataggttttg tttttcagag cttcaattta cttccaaaat tggatgcatt tcaaaatgtg
      481 gaattacctt tgatttatgc aggtgtatcg agagttgaac gatatgaaat agttaaaaaa
      541 tttttaacaa aagtggggtt agaagatcga atgcaccatc ggcctaacca gctctctggt
      601 ggacaaaccc agcgggttgc tattgctcga gctttggtta acgatccgtc tataatactt
      661 gccgatgaac ccaccggaaa tcttgacact cgctcaggcg aagagatcat gcaaatattt
      721 caaactctta atgatgaagg aaaaaccata attttggtca cccatgaaca tgatatatcc
      781 cttcatacca aaagaatcct ttcttttcgt gatggccaat tggttggaga cgaaaaagta
      841 gagaacccgg ttcgagccga agatagtctt aagacttata cggatcaaga agaaaaacag
      901 aaacttgaag aaagtcaatt atctaccaaa agttaaccga gaataaattt tcttatcttt
      961 ttaagaattg aacctcaggc caatttaaca gcacctagtg aagatgaaaa tacctattta
     1021 aagccgtcat tgcgaagagt tcaactggtt tttggttgga agacgtggca atcacaactg
     1081 acgctgcaaa cgtcatctta ggttttatat acagaagcca taaggatctc attttttcgt
     1141 gtttttaatc tttttctcag ggtaaccaag aataaacgaa agagaccctc cccgccgtaa
     1201 aacgacaccc ctccgaggag gagaattgtt gaattcattc caccgttggg gaagattcaa
     1261 gggggcgtgc ccttctcgtt tttttgattt ctttttactt tttttatttt ttcaggatag
     1321 accctcgtgc cactttggtg actccagatg gggatgaaaa tatgtatatg ccgaacaaat
     1381 tccccctttt ctaaaggagg taagggggat ttgaattttt tcctgttccg tcatggtgag
     1441 gagcaaagca acctggcaat ctcatccacc caatccgtca ttctgaggag cgtctggtgc
     1501 gacgcgagaa tctcatccgt catcaacgtc attgcgagga acgaagtgac gtggcaatct
     1561 catccttaaa gtttttataa agaagaaatt ataaaaaaat gagatcctca cgccctcaaa
     1621 aagtgagggc tcaggatgat ggcattttta atcattaatg tattttcagg atagaata
//
LOCUS       DAOSOL010000318         1674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_72601_length_1674_cov_3.099444, whole genome shotgun sequence.
ACCESSION   DAOSOL010000318 DAOSOL010000000
VERSION     DAOSOL010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72601_length_1674_cov_3.099444"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1674
                     /locus_tag="PK171_06610"
     CDS             19..>1674
                     /locus_tag="PK171_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOT05892.1"
                     /translation="MITGIVSRGDLPEMVITASRPPSFFGEIPKLIDELKENNWGKTY
                     LIFPSEKIELFAAILDEEKISYARDIKKPSQIFLLKGFPAEGFMLPDLGFSVFTTQEI
                     FGKDFTKPSKKRAESPIEEEMVSSLQPGDYIVHEEQGIGIFKGIKEMVLEGVHRVYLV
                     IEYAAGDILYVPVESASLVQKYIGSGDVKPKISRLGKDEWGKAKQRVAQSIQETAQEL
                     LEIYALRNLEQGHAFSLNSTWHKQLELSFPYIETPDQKQAIIDVERDMESTRPMDRLI
                     CGDVGYGKTEIAIRAAFKAVMDGKQVALLAPTTILSEQHYLTFKERLKRFPVRIAVIN
                     RFKSMLEQKQIVEGIKNNEIDILIGTHRLIQKDIQFHDLGLVIIDEEQRFGVMHKERL
                     KKMKSTVDVLTLTATPIPRTLYLSLTGIRDISVIETPPEGRKPVHTVVASRSQKMIKE
                     AIQYELERKGQVFYVSPRIKSLMKIYEELVLLFPECQIGIAHGRLSGPDLEKVMEKFY
                     NAEIDILLCTTIIEIGLDVPNANTLIVDPATLFGLSQLYQLRGR"
BASE COUNT          526 a          306 c          361 g          481 t
ORIGIN      
        1 ttatcacata tccctcaggt gattaccgga attgtttcga ggggagactt accggaaatg
       61 gttattacag cttctcgtcc gccttctttt tttggagaaa taccaaaatt gatcgatgaa
      121 ttgaaggaaa ataattgggg gaaaacctat ttaattttcc catctgaaaa gatcgaatta
      181 tttgcagcga tcttggatga ggaaaagatc tcctatgcta gagatattaa aaaaccgagc
      241 caaatttttc ttttaaaagg ttttccggca gaaggattta tgcttcccga tttgggtttt
      301 tcggttttca cgactcagga aatttttgga aaagatttta ctaaaccgag taaaaaaaga
      361 gcagaatcac caattgaaga agaaatggtg agctcacttc aacccgggga ttatattgtt
      421 catgaggaac aaggaatcgg aatttttaag ggaatcaaag aaatggtttt ggaaggggta
      481 caccgggttt atctggtcat tgaatatgca gctggtgata tcctctatgt tccagttgaa
      541 agtgcttcct tagtccaaaa gtatattgga agtggtgatg taaagccaaa aatatctcgg
      601 ttgggtaaag atgaatgggg aaaggcaaaa caaagagttg ctcaatcaat acaggaaact
      661 gctcaagaat tattagaaat atatgctctt cgcaacctgg aacaaggcca tgctttttct
      721 ctgaattcta cctggcacaa gcaacttgag ctatctttcc cctatattga aactcccgac
      781 caaaaacagg caattattga tgtggaacgt gatatggaat ccacacgacc aatggatcgg
      841 ctcatctgtg gcgatgtggg ctacgggaag actgagattg ccatacgagc ggcctttaaa
      901 gcagttatgg atggaaaaca agttgctttg ttggcaccaa caaccatttt gtccgagcag
      961 cattatctaa cctttaaaga gcgtttgaaa agatttccgg tacgaattgc agttataaac
     1021 cgctttaaat caatgctgga acaaaaacaa attgttgaag gaattaaaaa taatgaaata
     1081 gacatcttga tcggtaccca tcgactcatt caaaaagata ttcaatttca tgacctcgga
     1141 ttagtcatca ttgatgaaga acagcgattt ggggttatgc acaaagagcg gcttaaaaaa
     1201 atgaaatcaa ctgttgatgt cctcaccctc accgctaccc cgattccccg aaccttgtat
     1261 ctgtccctta ccggtattcg ggatatcagc gttatcgaaa cacctcctga aggccgtaag
     1321 ccggttcata ccgtggttgc gtctcgaagt caaaaaatga tcaaggaagc aattcaatac
     1381 gaactcgagc gaaagggtca agtattttat gtgagcccca gaattaaaag cttgatgaaa
     1441 atttatgaag agttggtatt actctttcct gagtgtcaaa ttggaattgc tcatggacga
     1501 ctctccgggc ctgatttaga aaaggttatg gagaagtttt ataatgctga aattgatatt
     1561 ttactttgta caacaattat cgagattggg ttagatgttc cgaatgccaa tactttaatt
     1621 gtagatccgg caactctctt tgggttgtct caactctatc aattgcgtgg tcgt
//
LOCUS       DAOSOL010000319         1663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_73300_length_1663_cov_2.036070, whole genome shotgun sequence.
ACCESSION   DAOSOL010000319 DAOSOL010000000
VERSION     DAOSOL010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73300_length_1663_cov_2.036070"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1663
                     /locus_tag="PK171_06615"
     CDS             <1..>1663
                     /locus_tag="PK171_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05893.1"
                     /translation="KEVDQSLNWLVVYRLWKELNKKTETVEVPGGLRRIECVTHILES
                     IRELLREEVSWSDFNRFMGCNDCFDDYLCSQIQDPTSWLCWIYRGFLKEYERYQLGDN
                     LQIPSLTAELIIKFGQNEKLHKWLQHNHFIFIGFFQFTPGQSQLLYSLDSNHCQIYII
                     KPWCGMPQFDEGFLSQRSLPYPPLREVKSVLIGEIAAGDTRHQYETIARELVLWSVGK
                     GQLSQWGQFPGWDEIVTIIPTQRRKIFQEVLQKYQIPFQFLQGYSAKESTLWGILNQL
                     RNAGQSNWPAEETLHLLTNPLLGEIDISVKETYQLSLHGIEEWRNLLKDFPQSLLKFD
                     QLVKSWRVIESCRTPRELYQVLYRIFTKTFNISFHASRLAGLDPSRDELVALITTFLK
                     EIEQKILQFSEDEILTFFAQQLEFTSSREVYGFLEAWVNHATLRPPLIEKGTLKVYLG
                     SPPALTEAPVVIISDIISAFWPGSFPNTPFLEDELKKKWNQNPELNQATLPTIHQKRD
                     EKRALFRRLIATGLQHTIVTYPLQDEAGRPTSLSPYYDEIFTGDDP"
BASE COUNT          515 a          311 c          325 g          512 t
ORIGIN      
        1 aaagaagtcg atcagagctt aaattggtta gtagtctatc gcctttggaa agaattgaat
       61 aaaaaaactg aaaccgttga agttccaggg ggattgcgaa gaatagagtg tgtaactcat
      121 atattggaaa gcatccgtga actcctccgt gaagaagtga gctggagcga ttttaatcgc
      181 tttatgggtt gtaatgattg ttttgatgat tatctttgta gtcaaattca ggatccaacc
      241 agttggttat gttggattta ccggggattt cttaaggaat atgagcgata tcaactcgga
      301 gataatcttc aaattccgag cttaactgct gaacttataa taaaattcgg gcaaaatgaa
      361 aaattacata agtggttgca acataatcat ttcattttta ttggattttt tcaatttacc
      421 ccagggcaaa gtcaattact gtatagtttg gactcgaacc actgtcagat ttatatcatc
      481 aagccttggt gtggtatgcc tcaatttgat gaaggattcc tttcccaacg cagccttcct
      541 tatcctccac tacgagaggt taagtctgtt ctaataggtg aaattgcagc tggggatacc
      601 cgtcatcagt atgaaaccat tgctcgtgag ttggttttat ggtcggtagg gaaggggcaa
      661 ctttcccaat ggggtcaatt tcccggttgg gatgaaatcg taaccattat tcccacccaa
      721 agaagaaaaa tttttcaaga agttttacaa aagtaccaga ttccctttca gtttcttcaa
      781 ggatattcag ctaaagaatc cactttgtgg ggaattctta accaattacg aaatgctggt
      841 caaagtaatt ggccagcaga agagacactt catcttctaa ccaatccatt attaggagaa
      901 atagatattt cagttaaaga aacctaccag ctatcgcttc atggaattga agaatggaga
      961 aatttattaa aggattttcc tcaatcttta ttaaaatttg atcaacttgt caagtcttgg
     1021 cgagttattg aatcctgccg aacgcctcga gaactgtatc aagtcctcta ccgaattttt
     1081 accaaaactt tcaacatttc ttttcatgct tcaagactcg ctggattaga tccttctcga
     1141 gatgaattgg ttgctttgat aactactttt ctgaaggaaa ttgagcaaaa aatccttcag
     1201 ttttctgaag atgaaatatt gacattcttt gctcaacaat tggaatttac ttcatccaga
     1261 gaagtttatg ggtttttgga ggcatgggtt aaccatgcca ctctccgtcc accccttatt
     1321 gaaaagggaa ccttaaaagt ctatctcggt tctccaccag ctttaaccga agcaccagtg
     1381 gtgataatta gtgatataat ttcagctttc tggccaggga gctttccaaa tactcctttt
     1441 ttggaagatg aattgaaaaa aaagtggaat caaaacccag aattaaatca agcaactcta
     1501 cctaccattc atcaaaagag agatgaaaaa agagctttat ttcgaaggct gattgccact
     1561 ggattacaac ataccattgt aacctacccg ttgcaggatg aagcaggacg gccaacatca
     1621 ctttctcctt attatgatga aatatttacg ggtgatgatc cat
//
LOCUS       DAOSOL010000320         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_73381_length_1661_cov_4.490037, whole genome shotgun sequence.
ACCESSION   DAOSOL010000320 DAOSOL010000000
VERSION     DAOSOL010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73381_length_1661_cov_4.490037"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PK171_06620"
     CDS             <1..1593
                     /locus_tag="PK171_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HOT05894.1"
                     /translation="VDTSLPIYKSANGSSSRTRSDWKLTIAERKRILLNNIYGVDIDA
                     QAVEVTKLSLLLKVLEEESEENVSKQLKLFEERALPSLHLNIKCGNSLIGTDIYADVQ
                     ATLDEPEVVKRINAFDWDREFLEIIQKGGFDAVIGNPPWGAEFSLYEQNYIRTHFKIA
                     TGRNLDSYALFIESGLKKLKEKGILGYITPDTFLRKDDHYPLRSLLLQDYIIKELIET
                     GPVFSKVRDTWGLVFIVQKQKPDDNQKISHKKISRFIVSTEERLERFGSEKWDNNSIV
                     AQNIWKNRPFKIIGYLSTEEDQQIIQKIEKMPKLGELSTFYKISRGEEGSKFLLEEKS
                     DGDFMMVIPRDVGRYSLQPGIKVMKKSLDANKIKNFYSYHKIWIIRIQKMRWRQRLVC
                     TYDERSNSAGMKTLQMIVSSENNDNLLKYLLGLLNSYLMNYYCINYLADDMNKSYLQK
                     IPIRTIDFSDPNDKARHDKMVTLVERMLALHKQKADVKTDHEKNLVERQIEATDKQID
                     TLVYEMYGLTEEEIRIVEGEGK"
BASE COUNT          553 a          299 c          373 g          436 t
ORIGIN      
        1 gtagatactt ctctccctat ctacaagtct gcgaacggga gctcgtcccg gaccaggagc
       61 gactggaaac tgacgattgc cgaacggaag cggatcctgc tgaacaatat ctacggcgtt
      121 gatatagacg cccaggcagt cgaggtgaca aagctctcgc ttctcctgaa ggtgctcgaa
      181 gaggagagtg aggagaatgt ctcaaagcag ctcaagctct tcgaagagcg ggcattgcca
      241 agcctgcatc tgaacatcaa gtgcgggaat tcacttatcg ggaccgacat ctatgctgat
      301 gttcaggcga cccttgatga accagaagta gtgaaaagga tcaatgcctt cgattgggac
      361 cgggaatttt tagagattat acaaaaaggt gggttcgatg cagtgattgg gaatccacca
      421 tggggggcag aattctccct ttatgaacaa aattacattc gaactcactt taaaattgct
      481 actgggagga atttagattc gtatgctctg ttcattgaat ctggattgaa aaaattgaaa
      541 gaaaaaggta ttctcggtta tattactcct gatacatttt taagaaaaga tgatcattat
      601 ccgttaagat ccttattact gcaagattac atcattaaag aattgataga aactggaccg
      661 gttttttcaa aagtaaggga tacttgggga ttagttttca tcgtccagaa acaaaaacca
      721 gatgacaatc aaaagatttc acacaaaaag attagtcgtt tcatagtgtc aactgaggaa
      781 agacttgaac gatttggaag tgaaaaatgg gataataatt caattgtagc tcaaaatatt
      841 tggaaaaatc ggccttttaa aattattggt tatttgtcca cagaagaaga tcaacagatt
      901 atccaaaaaa tcgaaaaaat gccgaaactc ggtgaattaa gcacttttta taaaatatca
      961 agaggcgaag agggcagtaa atttttattg gaggaaaaat cggatgggga ttttatgatg
     1021 gttattccta gggatgtagg ccgatactcc ctccaacccg gaataaaagt tatgaaaaaa
     1081 tctttggatg cgaataaaat caagaatttt tacagttatc ataagatttg gataatcaga
     1141 attcaaaaaa tgaggtggag acaaagactt gtctgcactt atgatgaacg aagcaattct
     1201 gccggaatga aaacgctaca aatgattgtt tcgtcagaaa ataacgataa tctgttgaaa
     1261 tatcttttgg gtcttctcaa ttcttacctt atgaattatt attgcattaa ctatcttgct
     1321 gacgatatga ataaatccta tttacaaaaa atccccatcc gtaccatcga cttctccgat
     1381 cccaacgaca aagctcgcca cgacaagatg gtcactctgg tcgagcggat gcttgccctc
     1441 cataagcaga aggccgatgt gaagaccgac catgagaaga acctggtcga acggcagatt
     1501 gaggccactg ataagcagat cgataccctg gtctacgaga tgtatggact gacggaggag
     1561 gagatccgga ttgtggaggg tgaagggaag tgaaatattt ctcccggtat atttcaaaga
     1621 gcccggtaat tcttggaaat aaatcagagt atgataaaaa a
//
LOCUS       DAOSOL010000321         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_73391_length_1661_cov_3.194271, whole genome shotgun sequence.
ACCESSION   DAOSOL010000321 DAOSOL010000000
VERSION     DAOSOL010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73391_length_1661_cov_3.194271"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1287)
                     /locus_tag="PK171_06625"
     CDS             complement(<1..1287)
                     /locus_tag="PK171_06625"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOT05895.1"
                     /translation="MLTEQMLQISQNLYQETENTNIQKAALRNIAYFAVPQFLLTESE
                     GSYPHDIQTIIQSEIDKSTGAVGRENSEIFNPDFNPFIKHDMDYSQFIIRGHYTRSEE
                     LKRYFMALMWYGQNYFLADQQTDLLQSLVITKQLFDNSYNHSKLIDLWETIYEPTVFY
                     VGLSDDLGPQDFKIILDTVYGKNIPYEDLADPIKLAAAQNMAEEMYSKKKRIKTELYL
                     IPSTAQFRFMGQRYIPDSEILQRLTKWTDTVPRIPLRPFPKGLDVMAVLGSRLASKIA
                     LEEHQNEGNIWKEYPENLEKLTVEFSQLTSNDWKTNLYYNWLFCLKSLLQLKSGYDYP
                     FYMRNQAWEKKNLITSLASWSQLRHDVILYGSPSGAEKGGSGIHSSKCQVEANPEFFG
                     RMLELLIHSRDGLQQRDLLTKPMQEKFEKFIDLVEFL"
     gene            complement(1206..>1661)
                     /locus_tag="PK171_06630"
     CDS             complement(1206..>1661)
                     /locus_tag="PK171_06630"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOT05896.1"
                     /translation="KNIEILLEKEKELLKQNFIEIDGKKRINTHNIINLWQFGEFSPE
                     DLDRLSQFGFTIFPCRYPDPKSENVEWQPAPYEQFFWLYENNNYYGVANFITTDAILQ
                     LYHIFLTLPYETSSRKNSIQLLRCSPNKCYKFLRIFTKKPKTPTSKRQP"
BASE COUNT          480 a          290 c          359 g          532 t
ORIGIN      
        1 aagaaattca accagatcaa taaatttctc gaatttttct tgcatcggtt tggttaacag
       61 atctctttgt tgtaatccat cgcgactatg gataagaagt tcaagcattc ggccaaaaaa
      121 ctcaggattg gcttcaacct ggcatttgga agagtggatt cccgatcctc ctttttcggc
      181 accagagggg cttccataaa gtattacatc atgcctgagt tgagaccaac tggccagcga
      241 agtgattaag tttttctttt cccaagcttg atttcgcata taaaagggat aatcatatcc
      301 acttttgagt tggagaagtg atttgaggca aaaaagccag ttgtaataga gattggtttt
      361 ccagtcgtta gaagtgagtt gcgaaaattc caccgttagt ttttctaagt tttctggata
      421 ttctttccaa atgttcccct cgttttgatg ttcttccaag gcaatttttg aggctaaacg
      481 agatcctaaa accgccataa catctaagcc cttggggaag ggacgaagcg ggatacgagg
      541 aacggtatcg gtccatttgg tgagacgctg caagatttca gaatcaggaa tataccgttg
      601 tcccatgaaa cgaaattggg cagtactcgg gatgagatag agttcggttt tgatgcgttt
      661 ctttttggag tacatttcct ccgccatatt ttgggctgca gcaagcttga ttgggtcggc
      721 aaggtcttca taaggaatat ttttcccata aacggtatca agaataattt taaagtcttg
      781 tggtccaagg tcatcggaaa gtccaacata aaacaccgtt ggttcgtaaa tcgtttccca
      841 aagatcgatc aattttgaat gattatagga attatcaaag agctgtttgg taataactaa
      901 tgattgaagg agatcggttt gctgatcggc caggaaataa ttctgtccat accacatcag
      961 cgccatgaaa taacgcttta attcttccga tcgagtataa tgaccgcgaa taataaactg
     1021 actatagtcc atatcgtgtt tgataaaagg attaaagtcg gggttaaaaa tctcagagtt
     1081 ttctcggcca actgctccgg tacttttatc gatttccgat tgaataatag tttggatgtc
     1141 atgaggatag gatccttctg attcggttaa caagaattgg ggaacggcaa aataggcaat
     1201 atttcttagg gctgcctttt ggatgttggt gttttcggtt tcttggtaaa gattctgaga
     1261 aatttgtagc atttgttcgg tgagcacctt aacaactgga tagagttttt ccgactcgag
     1321 gtttcgtagg gtaaagtcaa aaagatatga tagagctgaa gaatcgcatc agttgttata
     1381 aaattagcta caccatagta gttgttgttt tcataaagcc aaaaaaactg ttcataaggc
     1441 gcaggttgcc attctacatt ctcgctttta ggatcgggat aacgacacgg gaaaattgta
     1501 aatccgaatt gtgaaagtct atctaagtct tctggtgaaa attctccaaa ttgccaaaga
     1561 ttgataatat tatgagtgtt gattctcttt tttccatcga tttcaataaa attttgtttg
     1621 agaagttcct tttctttttc aagcaagatc tcaatgtttt t
//
LOCUS       DAOSOL010000322         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_73521_length_1659_cov_3.130299, whole genome shotgun sequence.
ACCESSION   DAOSOL010000322 DAOSOL010000000
VERSION     DAOSOL010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73521_length_1659_cov_3.130299"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PK171_06635"
     CDS             complement(<1..817)
                     /locus_tag="PK171_06635"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOT05897.1"
                     /translation="MDFLTKADLKEIVHKLKKLPFKVVLAILFGSSTRRTLSRDSDID
                     ILIISDEIPPHRHRRQSEISLIKKLINIDRPLDILLLTREECFSNFNNHNPLFLDIAT
                     EGKVLIAEDEFLEELIEETKKYIHDQGIEKTEDGWKFPVPYRKEAFLSQVSNRQFAHA
                     MLDDAKRVFEIGIIILEKEYFDKAVFHFQQSVEKSLKAVLIMMGVFVKSHFIGKALIK
                     EIEEKQFPQLWMNKLRHIAQLSTEIEPEVTWSRYPGIDNNSLWIPAEEYSLEDA"
     gene            complement(929..1333)
                     /locus_tag="PK171_06640"
     CDS             complement(929..1333)
                     /locus_tag="PK171_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOT05898.1"
                     /translation="MESGIVLDSFAVLAYLQAEKGAQQVKELLHQAANGQLSVQMTVI
                     NLGEVYYITSRVYGQERAEEVLAMLKHLPIKLVVADEELTLEVSRIKAKHPLSYADAF
                     AVALAQRHGVPVVTGDPEFSCIETLISVKWLK"
     gene            complement(1337..1615)
                     /locus_tag="PK171_06645"
     CDS             complement(1337..1615)
                     /locus_tag="PK171_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOT05899.1"
                     /translation="MGRAKVSPKGQIVIPASIRKKHGIKPGSIMEITEGDDRIILKLA
                     PKDPVEEAMGMLKGDISLTKELLQTRDEERKAEEQKATRPFDRKDKGE"
BASE COUNT          472 a          360 c          277 g          550 t
ORIGIN      
        1 cggcatcttc gagtgaatat tcttcagccg gaatccaaag gctgttattg tcaatacccg
       61 gatacctact ccaagttacc tcgggttcaa tttcagtact caattgagct atatgacgca
      121 gtttattcat ccataattgc ggaaattgtt tttcttcgat ttctttgatc agagctttcc
      181 caataaaatg agatttaaca aaaacaccca tcatgatcag aactgctttt aatgactttt
      241 caaccgattg ttggaaatgg aacacagcct tatcaaaata ttccttttct aaaattataa
      301 ttccgatttc aaaaaccctc ttggcatcat cgagcatggc atgggcaaac tgccggttgc
      361 ttacttgaga caaaaaggct tctttacgat atggtactgg aaatttccat ccatcttcag
      421 ttttttcaat tccttgatca tgaatatact ttttggtttc ttcgatgagt tcttctaaaa
      481 attcatcttc agcaattaag actttcccct cagtagcaat atccaaaaat agaggattgt
      541 ggttattaaa attagaaaaa cattcttctc gagtaaggag cagaatatca agaggccggt
      601 ctatgtttat taattttttt ataagtgaaa tttcactctg tcttcgatgt cgatgaggag
      661 gtatctcatc agaaatgatt aaaatatcaa tatccgaatc tctacttagt gtcctacgag
      721 tcgaggagcc aaacaaaata gccaatacta ctttgaatgg caattttttt aatttatgaa
      781 caatttcctt tagatcggct ttggttaaaa aatccatttt cacacctctc gtggttaagt
      841 cttgattctt gaattttaac aaattttaat ctcattcatc cacctacgag tttggtaaat
      901 caaataaaaa agagcttgtc tcctttttct acttcagcca tttaacagaa atcagagttt
      961 caatacaaga aaattctgga tccccagtta ctactggtac accatgacgt tgagccaggg
     1021 caactgcaaa ggcatcggca taagaaagag gatgtttagc tttaatccgg gaaacttcta
     1081 acgttaattc ttcatcggca acaaccagct ttatgggtaa gtgtttcaac atggccaaca
     1141 cttcctcagc ccgctcttgg ccataaaccc gtgaggttat ataatatacc tctccgaggt
     1201 taatgacagt catctgaacc gaaagttgac cgttagccgc ctgatgaaga agttccttca
     1261 cctgttgggc tcctttttct gcttgaagat aggctaatac cgcaaagcta tctaaaacga
     1321 tcccgctctc caccaatcat tcacccttgt ctttacgatc aaacggccgg gtggctttct
     1381 gttcttctgc tttccgttct tcgtctcttg tctgcagcag ttccttggtc aaggaaatat
     1441 cccctttcag cattcccatt gcttcttcta ccggatcttt aggagcgagt ttcaggataa
     1501 tacgatcatc accttcggta atctccatta tcgaaccagg tttaatccca tgttttttcc
     1561 gaattgaagc aggtataaca atttgtccct tgggggatac ttttgctctt cccatgtaca
     1621 actccttatt aaaatgtaat actttatttt atatagttt
//
LOCUS       DAOSOL010000323         1657 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_73656_length_1657_cov_3.916355, whole genome shotgun sequence.
ACCESSION   DAOSOL010000323 DAOSOL010000000
VERSION     DAOSOL010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73656_length_1657_cov_3.916355"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PK171_06650"
     CDS             complement(<1..411)
                     /locus_tag="PK171_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05900.1"
                     /translation="MIEAGKRDYIIQAHHIFKTFPGVKALSNVSFDLHQSEIHCIVGE
                     NGAGKSTFIKILTGSLRPDSGELMVHGKNYKYLTPYLSQTLGIQVIYQENIVVPQMTV
                     AENVFVGREKATRFGLIRYQETIQATQKIISSLKI"
     gene            complement(499..1500)
                     /locus_tag="PK171_06655"
     CDS             complement(499..1500)
                     /locus_tag="PK171_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOT05901.1"
                     /translation="MKKPVVKGLGVVLLVALFSLLVFGVVFAEENEKLTFGYIAFSRK
                     DIWNNYSILAFEYAAKQKGIETIVLDPEGNLEKAVSSMEDLITKKVGGISVFTMTPEL
                     DVTMAEMSNAAGIPITFENSLPAENTPNYISCVACQYDDIGYAAGKYINEAYPGSKLF
                     YVMGAPGMGITELYEQGLHKALDEFGKVELVSTQPTDWGAEQAMNVTQNAIQSGLEFD
                     VIFANNEQMATGVINALKDAGLFGKVKVVATGGGPHGLEMIEKGELDATMSAPVSLQG
                     LITFKSLYEFITKGKQPEKFIPLPIIPIFKENLAEAISWEITDENYERALNYIGGIE"
BASE COUNT          486 a          353 c          294 g          524 t
ORIGIN      
        1 aattttcaag gagctaatga ttttttgagt tgcctgaatg gtctcttggt agcggatcaa
       61 tccaaagcga gtggcttttt ctctgccaac aaagacattc tctgcaacgg tcatttgtgg
      121 cacaacaata ttctcttggt aaataacctg aattcccaag gtttgagaaa gatagggagt
      181 aaggtattta taatttttac catgaaccat gagttctccg ctatcaggcc gtaaactacc
      241 agtgagaatt ttgataaagg ttgattttcc ggcaccattt tctccaacaa tacaatggat
      301 ctcactctgg tggagatcaa atgaaacatt ggaaagagct ttcactcctg gaaaggtttt
      361 aaatatatga tgggcttgga taatatagtc tcttttccct gcttctatca tgactcattt
      421 acctttttca aatggtctat aattaaaaag atgtccttcc ccataagagg aaggacatca
      481 tgaatccaac taattaaatt attcaatacc accaatgtaa tttaaagccc gttcatagtt
      541 ttcatcggta atttcccagg atatagcctc ggctaaattc tccttgaaaa ttggaataat
      601 cggaagaggt atgaattttt ccggttgctt tcctttggtt ataaattcat agagactctt
      661 gaaagtaatc aagccttgca gtgaaactgg tgcactcatg gttgcatcta attcgccctt
      721 ctcaatcatt tccagaccat gtggtccacc accagtagca acgactttga cctttccaaa
      781 taaaccggcg tcttttaaag cattgataac accagttgcc atttgttcgt tattagcaaa
      841 aataacatcg aactccagac cggattgaat ggcattttgg gtaacgttca ttgcttgctc
      901 agcaccccaa tcggttggtt gggttgaaac cagctctact ttaccaaatt catcgagtgc
      961 cttatggagt ccttgctcat aaagttcggt aattcccata cctggtgctc ccattacata
     1021 aaacagttta cttcctggat aggcttcatt gatatatttc cctgctgcat aaccaatatc
     1081 atcatattgg caggccacac aggaaatgta gttgggggta ttttcggcag gaagcgagtt
     1141 ttcaaaggta atcggtatcc cggcagcgtt tgacatctcg gccatagtaa catccaattc
     1201 gggagtcatg gtgaaaaccg atattccacc aaccttttta gtgatgaggt cttccattga
     1261 agaaacagct ttttccagat ttccttctgg atcaaggacg atagtctcaa tgcctttttg
     1321 tttggctgcg tattcaaagg ctaaaattga ataattattc caaatgtcct ttctactaaa
     1381 agcaatataa ccgaaggtta gcttttcatt ctcttcagca aaaacaactc cgaagacaag
     1441 aagcgagaag agcgcgacta ataaaaccac acccaatccc ttcactacgg gttttttcat
     1501 aatatcttac ctcctcataa taatattgat actcaataat attatacctt taattgctaa
     1561 atttccaact ggaaatccgt ttagagcatc tcagcaccta caattaattt aacaatctcg
     1621 tcgggagtag tttgtttttt tatcctttca ccaactt
//
LOCUS       DAOSOL010000324         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_73839_length_1654_cov_3.517824, whole genome shotgun sequence.
ACCESSION   DAOSOL010000324 DAOSOL010000000
VERSION     DAOSOL010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73839_length_1654_cov_3.517824"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /gene="cimA"
                     /locus_tag="PK171_06660"
     CDS             <1..1339
                     /gene="cimA"
                     /locus_tag="PK171_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOT05902.1"
                     /translation="KPDQDKNLRLLLEAGSPVITIFGKSWTLHVKRALRTTLDENLKM
                     IDDSINFLKSAGKTVFFDAEHFFDGYKQDRDYALKTIEVAYNAGADAIILCDTNGGAL
                     PYEIEPIFSQVMSQLPKSFEIGMHAHNDGGVAVANSLVAISCGANQIQGTFNGYGERC
                     GNANLCSIIPNMVLKMGISCLSEEKLKNLVDVSRFIDETANMRPNRYQPFVGKSAFTH
                     KGGIHASAIMRDPLTYEHIIPDLVGNQRRILVSEQAGKSNILYRAHQVGVDLQPDDSR
                     VTSLIEKIKESESYGYQYEGADASFEMMLREALGETMNFFTLQGFRVIVEKRGDEGVI
                     TEATIKLNVENQMVHTAAEGDGPVHALDNALRKALENLYPQLKRIRLSDYKVRVLNEK
                     DGTAAKIRVLIQTTDGSHTWGTVGVSTDIIEASWEALEDSVKYGLWQKTREEK"
     gene            1312..>1654
                     /locus_tag="PK171_06665"
     CDS             1312..>1654
                     /locus_tag="PK171_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05903.1"
                     /translation="MAKNQRRKIKSKIIYKGFTKINDVHFDWNVKPDNFFNGFPVPPF
                     VSWTINTDRISSFNRNILQPNQQIVWIGKDDIIQFLECFYAESELIQNHQSYHFCSSS
                     HPEYLYKLSHSV"
BASE COUNT          527 a          293 c          352 g          482 t
ORIGIN      
        1 caaaccagat caagataaaa accttcggct tctccttgaa gccggttcgc cggttattac
       61 gatattcgga aaatcatgga ccctacacgt aaagcgagct ctacgtacca cattggatga
      121 aaatctaaag atgattgatg attccatcaa cttcttaaag tcagcaggaa aaacggtttt
      181 ttttgatgcc gagcattttt ttgatgggta taaacaggat cgggattacg cattaaaaac
      241 catagaagtc gcctataatg ccggagctga tgcaattatt ctttgtgata ccaatggtgg
      301 agcgctaccc tatgaaatag agcctatttt ttcacaagtt atgtctcaac tcccgaaatc
      361 ttttgaaatt gggatgcatg ctcataacga cggtggagtt gctgtagcca acagcttagt
      421 tgcaataagc tgtggagcaa accaaattca aggaactttt aatggatatg gagaacgttg
      481 tgggaacgcc aatctttgca gcattattcc caatatggtt ttaaaaatgg ggatttcttg
      541 tttatctgaa gaaaaattaa aaaatttggt agatgtttcg cgattcattg acgaaaccgc
      601 aaatatgcga cctaaccgtt atcaaccctt cgtcggtaag agtgccttta ctcacaaagg
      661 aggtattcat gccagtgcaa tcatgaggga tccgttaacc tatgaacata tcattccaga
      721 cctagtgggt aatcaaaggc gaattttagt ttcagagcaa gctggcaaga gtaatatcct
      781 ttatcgagct catcaggtag gggttgattt gcaacccgat gattctcggg tgacttcctt
      841 aatagagaag ataaaagagt ctgaatctta tggatatcag tatgaaggag ccgatgcttc
      901 ttttgagatg atgctccgtg aagccttagg tgaaaccatg aattttttca ccttacaagg
      961 atttcgggta atcgtagaaa aaagaggaga cgaaggagtg attaccgagg caaccattaa
     1021 gctaaatgtt gaaaaccaaa tggttcatac tgctgcagaa ggcgatggtc cagttcatgc
     1081 tttggataat gctcttcgga aagctcttga gaatttatat cctcagttaa aaagaatccg
     1141 tttaagcgat tacaaggttc gggtcttaaa tgaaaaagac ggaactgcgg ctaaaattcg
     1201 ggttttaata cagacaaccg atggtagcca tacttgggga acggttggtg tttcaactga
     1261 cataatagaa gcgagctggg aggctttgga agatagtgta aaatatggac tatggcaaaa
     1321 aaccagagaa gaaaaataaa atctaaaata atttataaag gatttaccaa aattaatgac
     1381 gttcattttg attggaacgt caaaccggat aattttttta atggttttcc tgttcctccc
     1441 tttgtgagtt ggacaattaa taccgatcgg attagcagct ttaatcgcaa tattctccag
     1501 ccaaaccaac agattgtatg gattggcaaa gatgatatta ttcaattttt agaatgtttt
     1561 tatgctgaat ctgagttgat acaaaaccat caaagctatc atttttgttc gtcatctcac
     1621 ccagaatatc tttataaact aagccattct gtcg
//
LOCUS       DAOSOL010000325         1637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_74911_length_1637_cov_3.300253, whole genome shotgun sequence.
ACCESSION   DAOSOL010000325 DAOSOL010000000
VERSION     DAOSOL010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74911_length_1637_cov_3.300253"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PK171_06670"
     CDS             complement(<1..398)
                     /locus_tag="PK171_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05904.1"
                     /translation="MDKMTSRERFRKALNHQEPDRVPIDVGQDFHNGIHEVAYRNLLA
                     FLGEEDDIRLYDRIQHLAVCKESILERLHADTRYVFANAPSNWQIKVHADSSWADEWG
                     VVRKNVGLYDESIQCPLAGASIDEIKSYQMP"
     gene            703..1413
                     /locus_tag="PK171_06675"
     CDS             703..1413
                     /locus_tag="PK171_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05905.1"
                     /translation="MESYGDKITQSLLEEGASLVGFANLSGLSPDITKSFPYAISISI
                     ALNPAIIAKINQGPTADYFEEYKRVNLFLSDLGKFAALILVNLGFKAFPIEPTTEHFD
                     LNTLSTFLPHKTIATRAGLGWIGKSALLTTKQYGSAIRLTSVLTDCPLPTAKPINQSK
                     CGDCRLCVESCPGHAPLGNNWDINHPRESFFNAFTCRETAIKLSQPVGIEGTVCGICI
                     VSCPWTQHYLMRQKPKSC"
BASE COUNT          512 a          337 c          295 g          493 t
ORIGIN      
        1 ggcatttggt agcttttaat ttcatcaata gatgcacctg ccaaagggca ctgaatgctc
       61 tcgtcgtata gcccaacatt ttttcgaacg actccccatt catcagccca agaggagtca
      121 gcatgaactt ttatttgcca attggaagga gcattggcaa atacatacct ggtatcagca
      181 tgcaacctct ctaaaatgct ctccttacaa actgccaaat gttggatccg atcataaagc
      241 cggatatcat cctcttctcc caaaaaggca agaagatttc gataggcaac ttcgtggatt
      301 ccattatgaa agtcctgtcc aacatcgata ggaacacggt cgggttcttg gtgatttaat
      361 gcctttcgaa aacgttctct tgaggtcatt ttatccattt tttccccttt ttgcctatgg
      421 ttttaaattg ttatacaaac tgcttgcaaa aacctaccaa ctttgtatcg aaaatgaaat
      481 attaaacttt gtcaactttc cgttaaaaag tgcgaaataa caagctattc tctcgtttac
      541 tataacatat tttgacccaa tcttatctca atacctaatt aatttccgat ttccaaaaaa
      601 aattatggaa ggagtttatt aattaaacca gttaatccgt ttaaaggtta taatttaaaa
      661 aaagaaataa aatccatttt aatcaaacag gagcgatcat gaatggaatc atacggtgat
      721 aaaataactc agtcactttt agaagaagga gcttctttgg ttggctttgc taatctaagt
      781 ggtctttctc ctgatattac caaatcattt ccttatgcta tttcaatttc aattgctctg
      841 aacccagcaa taatagcaaa aattaaccag ggacctactg cagactattt tgaagaatat
      901 aaaagagtga atctttttct atccgattta ggaaaatttg ctgctttaat cctggtcaat
      961 ctgggattta aagccttccc tatcgaaccg acaactgagc atttcgatct taatactctg
     1021 tcgacttttc tgccccataa aacaatagca actcgcgctg ggcttggttg gataggaaaa
     1081 tcagctctcc ttaccaccaa gcaatatgga agtgccatac gtcttacttc ggtgctcact
     1141 gattgtccac taccgactgc gaaacccatc aatcaaagta aatgtggtga ctgtaggttg
     1201 tgtgttgaaa gttgtcccgg ccatgctcca ttgggaaaca actgggatat aaatcatccc
     1261 cgtgaaagct tcttcaatgc tttcacctgc cgggaaactg ccatcaagct ctcccaaccg
     1321 gttggaatcg agggaacggt ctgtggaatt tgtattgtct cctgtccttg gacccagcat
     1381 tatcttatga ggcaaaagcc aaaatcgtgt tgatttaaag tcttgaatta aggaataaga
     1441 aaagatgaga tcctcacgca ttacaataca ctcttaaaat atagtattag tgaaataact
     1501 gtagttttga tttgggataa ggatctatcc taaaaatacc gaaattggtg aaaaaagaat
     1561 aaacaaaaaa agagaaagaa tcaattcaac aattcccctc cttggagggg tgccgtttac
     1621 ggcggggagg gtgcttt
//
LOCUS       DAOSOL010000326         1621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_75947_length_1621_cov_2.457216, whole genome shotgun sequence.
ACCESSION   DAOSOL010000326 DAOSOL010000000
VERSION     DAOSOL010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75947_length_1621_cov_2.457216"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PK171_06680"
     CDS             <1..740
                     /locus_tag="PK171_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05906.1"
                     /translation="LETNTVNVTKLDKKEEVPVATLTYRDGHFYSPDDSRFSFLTVNG
                     HHLLMVSIFNQVVEMPIGQQLSKLDEPLNLGIDVDGKRWLRRNVLPGEAFNPEHIMTS
                     QLIEDLPGYVVFQGVKQILSPQYAGIPVGAVRDQTDLTLFEDNGHTWARVFDFLYSPI
                     ETALPLETGENEVTIGNLNYSEWLSVPEECIVDIQKPEQGRVFIFSPEGSVLYDSLSD
                     SGKVFIPSGGFIELIGHAGDVFNVTRN"
BASE COUNT          502 a          288 c          353 g          478 t
ORIGIN      
        1 atcttgaaac taataccgtt aatgtaacca agctggataa aaaagaagaa gtacctgttg
       61 ctaccttgac ttaccgggac gggcattttt acagccctga tgatagtcgt ttttcttttc
      121 ttacagtgaa tggccatcat ctgctgatgg tctccatttt taatcaagtc gtcgagatgc
      181 ctatcggtca gcagctcagt aaactcgatg aacctctgaa tctgggaatc gatgtcgatg
      241 ggaaacgctg gctgaggcga aacgtgctcc ctggagaagc attcaaccca gaacacatca
      301 tgacctctca gctcattgaa gaccttcccg ggtatgtggt tttccaaggg gtcaaacaga
      361 tcttgtctcc acagtatgct ggtattccgg tcggagctgt tcgagaccag accgatttaa
      421 ctctgttcga ggataacgga cacacctggg caagggtttt tgattttctc tacagtccca
      481 tcgaaaccgc tcttccactg gaaacagggg aaaatgaagt gactatcggt aatctcaatt
      541 acagcgagtg gctatcggta ccagaagaat gcattgttga catccagaaa cccgagcagg
      601 gtagggtatt tatcttttct ccggaaggat cggtcctcta cgacagcctg agtgatagcg
      661 gtaaagtctt tattccatct ggtggtttca ttgagctcat tggacacgcc ggtgatgtat
      721 tcaatgtaac caggaattag ttaatggtga atgatagcca ataagcggtg agaagtgatc
      781 gttaaactat ttgaataacc tggtattggg ataggaaagt ttgcaataaa actttcctat
      841 cccgcattaa aattatattg tgtatttcaa gtcacaagtg gagctcattc tcacctgact
      901 ggattaaagg ataattccta ttctgggaaa agcatgaaaa tcaatcgaaa atcggaactt
      961 cttcaactgc tttctggaaa gtcttgctgg attagttgac ttaagaaacc ttatctccaa
     1021 cacctaagtg cactgatagg tctccttgca tatactctct aaaggattag tagaggtaat
     1081 ttccatctta tcctatggaa gaagaccaga atttattggg aatctttaag tgtgtatggt
     1141 ttcctcaacc gagtttatat atttttcagt taatttaatg gttaaaaaca ataatctcta
     1201 tgagataatt gttcaattat gaaggtagga tatgaaccat agcagttgaa aaaatttaaa
     1261 aaattgaatt ttatctgaat tttcattatg aattagtgag agagtaaaag tatggaattg
     1321 gtaaaaaaat ggaaggagga gaaagggaaa ggggtgagag ggagataaat ttccgcccct
     1381 gcgtttttta aattcttttg taggagcttg atttattgta cctgaatgtt tctgaaggag
     1441 ataaaagatc aatgatattt ttctactaga ttactttttt aagttagaag ttcaccacat
     1501 cgtttcacaa tcggggatga aagggggatt tgaaagagtt aagaaaaaaa tcgcagcccg
     1561 aaccatctca ctttcttcaa aggggatgaa aaaaccatga taaaaatcat caaagaagaa
     1621 t
//
LOCUS       DAOSOL010000327         1618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_76110_length_1618_cov_4.083173, whole genome shotgun sequence.
ACCESSION   DAOSOL010000327 DAOSOL010000000
VERSION     DAOSOL010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76110_length_1618_cov_4.083173"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PK171_06685"
     CDS             complement(<1..257)
                     /locus_tag="PK171_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT05907.1"
                     /translation="MYCLAIETSCDDTCVAIIKNGYTILSNVISSQIDIHRRFGGVVP
                     EVASRRHLEYLIPALEQALQQANLTLKEIDLFAITRGPGLTG"
     gene            complement(273..746)
                     /gene="rimI"
                     /locus_tag="PK171_06690"
     CDS             complement(273..746)
                     /gene="rimI"
                     /locus_tag="PK171_06690"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT05908.1"
                     /translation="MNNSPFPAEPYVIEKMKPIHLSAVLAIEKKSFPQPWSFSLFMSE
                     FTNRLATYLVLRIKRKVIGYIGYWYLFEEAHITTFAIHPDYRRQGLGKKFLQYALNSI
                     LNQGCQEVFLEVRVSNFPAQNLYRSLDFTAIGIRTKYYSDGEDAIIMKKIISKEG"
     gene            complement(739..1452)
                     /gene="tsaB"
                     /locus_tag="PK171_06695"
     CDS             complement(739..1452)
                     /gene="tsaB"
                     /locus_tag="PK171_06695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT05909.1"
                     /translation="MDNNQTIPNTLALDTSTPWLTLTLGNSAEVIYQHRSQVMTDHSR
                     VLIQSLEEIQKKGFFSTFPPERIVIGRGPGSFTGIKIANITGRTLAYSLNCPLYGFST
                     LEMLAHQGSKLITPHKGLIILPVIFHKKNEVFWSEFKLGFQKLPDHQVKIQIGPYSDI
                     IKLYSSKDVLIITPWQELYNLFQNNQFQCLHPDSSQPDSINLIELIESRDIPIQKNNS
                     EFFEENTRPLYGSRVFEHE"
BASE COUNT          519 a          281 c          323 g          495 t
ORIGIN      
        1 ccagttaatc ctggaccacg agttattgcg aacaaatcaa tttctttaag agtaagatta
       61 gcctgttgca aggcctgttc taaagccgga atcaaatatt ccaaatggcg tcgggatgcg
      121 acttctggaa caactccacc aaaacgacga tgaatatcga tttgactgga tattacattg
      181 gagaggatgg tatacccatt ttttataata gcgacacaag tatcgtcgca actggtttca
      241 attgccaagc agtacataac aatcctttct ttttaacctt ctttgctgat gatttttttc
      301 atgataatag catcttcacc atcactgtaa tattttgttc gaattccaat ggcagtaaaa
      361 tccagtgaac gataaagatt ttgggcaggg aaattagaaa ctcttacctc aagaaaaact
      421 tcctgacaac cctgattcaa aattgaattc agagcatatt gaaggaattt tttccccaat
      481 ccttgacggc gataatcggg gtgaatggca aaggtggtaa tgtgggcttc ttcgaaaaga
      541 taccaataac caatatatcc aatcactttt ctcttaattc gaagaaccag gtaggtcgcc
      601 agtcggttag taaattcact cataaaaaga gaaaaactcc agggttgggg gaaagatttc
      661 ttttctatag ctaaaactgc tgataaatgg attggtttca ttttttcgat gacatagggt
      721 tcagctggga aaggagaatt attcatgttc aaagactcgt gatccatata gaggtcgagt
      781 attttcttca aagaattcgg aattattttt ttgaatagga atatcccggc tttcaattaa
      841 ttcaataagg ttgatggaat caggttgtga actatcaggg tgaaggcact gaaattgatt
      901 gttttgaaaa aggttataaa gttcctgcca aggagtaata atcaaaacat ctttagaaga
      961 ataaagcttt ataatatctg aataaggtcc gatttgaatt ttgacttggt gatccggtag
     1021 tttttgaaag cctagtttaa attctgacca gaagacttca tttttcttat gaaaaataac
     1081 tggaagaata atcaagcctt tgtgaggggt tattagttta cttccctggt gggctaacat
     1141 ctccaaagtg gaaaatccat ataagggaca attcaagctg tatgccaaag ttcggccggt
     1201 gatattggct atttttatac cggtaaaaga accaggacct cgaccgataa cgatccgttc
     1261 tggtggaaag gttgaaaaga atcccttttt ctgtatttcc tccaaagatt gaatcaaaac
     1321 ccgggaatgg tcggtcatga cctgggaacg atgctgatag ataacttcag cactatttcc
     1381 taaagttaaa gtcagccaag gagtgctagt atccaaggct aaagtatttg gtattgtttg
     1441 attattgtcc ataattttga taaaagattt cgatgagtcg gtttttttca tcggtaacag
     1501 ttaatttgac tacataataa ggaggaagaa aatgagctaa aaatcgttca ccccattcaa
     1561 ttacagtaaa ggaatttgaa gtaaaatatt cctcgatccc caactgctca acctcctg
//
LOCUS       DAOSOL010000328         1611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_76550_length_1611_cov_3.420951, whole genome shotgun sequence.
ACCESSION   DAOSOL010000328 DAOSOL010000000
VERSION     DAOSOL010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76550_length_1611_cov_3.420951"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PK171_06700"
     CDS             complement(<1..704)
                     /locus_tag="PK171_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05910.1"
                     /translation="MKKNGSISWWITGIILTIGVFVGGSPVFPLNELPQQFTITVSET
                     EVRPGQEVLVSYTASSDWSANAWVGLIPANIPLGDQKIADENDLDYRHLKNTRQGKLS
                     FIIPDVEGIFNFRLYPSDDDSYSEVAVSESIKVVREPIIPLDANQNGLKTENPVDELP
                     SFPDQDVNETSLSQLPGIGQITTNIPLLTPTPIPTLSEASSFPINSEKKDELPSLSPT
                     ESSLPELSLPPVDSNPP"
     gene            871..1215
                     /locus_tag="PK171_06705"
     CDS             871..1215
                     /locus_tag="PK171_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT05911.1"
                     /translation="MANYIEPVEELSNADRDIHRALNSLKEEIEAIDWYYQRAAATAE
                     SEIKTILIHNMEEEIEHASMILEWLRRNMEGWNENLKTYLFATEPIGKIETSEKSAEP
                     EIKGDLGIGNLK"
     gene            1269..>1611
                     /locus_tag="PK171_06710"
     CDS             1269..>1611
                     /locus_tag="PK171_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOT05912.1"
                     /translation="MDILKRNLAPITDQAWQEIDRQAKKVFQNQLTARHFVDVIGPKG
                     WNYAGVGLGRLNVPENQREGEVRFGIHMVQPLVEIRTSFQLDLWELDNISRGAIDIDF
                     TPLIDAAKQTAL"
BASE COUNT          526 a          287 c          342 g          456 t
ORIGIN      
        1 ggtggattgg aatcgactgg aggaagtgat aattctggga gggatgattc ggttggtgaa
       61 agggagggga gttcatcctt cttctcagaa tttattggaa aagaggatgc ttctgaaaga
      121 gttggaatcg gagtcggggt aagaagggga atattggttg taatttgacc aattcccggc
      181 agttgggaaa gagaggtttc attaacatcc tgatcaggaa aggaaggaag ttcatcaaca
      241 ggattctccg ttttcaatcc attctgatta gcatccaaag gaataattgg ttctctgacc
      301 actttgatag actcgctcac tgcaacttct gaatagctat catcatcgct gggatataag
      361 cgaaaattaa aaattccttc aacatcagga ataataaagg atagcttccc ctgacgggta
      421 ttttttaaat gtcgataatc aagatcgttt tcatcagcaa ttttttggtc acccaaagga
      481 atatttgcag gtatcaaacc tacccaagca tttgcgctcc aatcggaaga agcagtatag
      541 ctgactaaga cttcttgacc cggtctaact tccgtttcag aaacggtaat ggtaaattgt
      601 tgaggcaatt catttaatgg aaaaactgga cttccaccaa cgaaaactcc gatggtaaga
      661 ataattccgg tgatccacca actgatcgaa ccattttttt tcatattcat ttccttcctt
      721 tttattcgat tcattcctga tcctacccca agaaaccctt agcacaaaac ttgaatagaa
      781 gcaaagttat agctactatg ataccaatca attcactcat gaaaaagtat ataatagaat
      841 tattgatgat tgataaggag gtgttctaat ttggccaatt atattgaacc ggttgaagaa
      901 ctttccaatg ctgatcgaga cattcatcga gctctaaata gtttaaaaga agaaattgaa
      961 gctattgatt ggtattatca aagagctgcc gcaactgccg agtcagaaat aaaaaccatt
     1021 ctcattcata atatggaaga agaaattgag catgccagca tgattttaga atggttacgg
     1081 cgaaatatgg aaggatggaa tgagaattta aaaacctatt tgtttgccac tgaaccaatc
     1141 ggaaaaattg aaacatcgga aaaatctgcc gaacctgaaa taaagggtga tttggggatt
     1201 ggtaacctga agtagtcctt ctgaataaag gaccggaaat aatttcttca tttatcaagg
     1261 aggataccat ggacatttta aaaagaaatt tagcgcccat aaccgatcaa gcctggcagg
     1321 aaattgatcg tcaagccaaa aaagtctttc aaaatcaact taccgctcgt cattttgttg
     1381 atgttattgg cccaaagggg tggaattatg ctggagttgg attggggagg ctcaacgtac
     1441 cagaaaacca aagagaagga gaggttcgat ttgggataca tatggttcaa ccgttagttg
     1501 aaattcgaac ttcgtttcaa ctcgacctct gggaattaga taatattagt cgtggagcaa
     1561 ttgatattga cttcactccc ttaattgacg ccgccaaaca aactgctctt t
//
LOCUS       DAOSOL010000329         1610 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_76602_length_1610_cov_3.236656, whole genome shotgun sequence.
ACCESSION   DAOSOL010000329 DAOSOL010000000
VERSION     DAOSOL010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76602_length_1610_cov_3.236656"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK171_06715"
     CDS             <1..233
                     /locus_tag="PK171_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05913.1"
                     /translation="TSFPELARLAQKYSDQPRKVTLGGGKLFPRGETLDISLAMKELG
                     YTPQYRMEKAVAEYSEWIHKQLRSKKTVLDYK"
     gene            433..1248
                     /locus_tag="PK171_06720"
     CDS             433..1248
                     /locus_tag="PK171_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOT05914.1"
                     /translation="MKIILLADLEGVSGVVDNEQQAKPGAPLYQEAREYLLSDVNAVI
                     EGALDGGATEITIFDMHYYGLNLNLSKIHPQTWVFMGKPRKIYPLLKEVYQKADGFMM
                     VGFHAMAQTPGGLLTHTYDHSIKSLYLNGILMGEIGMEAAIAGFYGVPLIFVAGDSKG
                     TEETESLLGDIPTVTVKWAVNEHSALCLPIEVTHNEIRKTAKESLEKITEFKPYVLEP
                     PYHIEVEFFLPEKAEKMEKLAGVARIDELTVQAQGDDLPLLWEEFIGFCQNYQ"
BASE COUNT          476 a          341 c          319 g          474 t
ORIGIN      
        1 taaccagctt tccggaatta gcccggttag ctcagaaata cagtgatcaa cctcgaaaag
       61 taacgcttgg aggaggcaaa ctcttccctc gcggtgaaac tctcgatata tctttggcta
      121 tgaaagagct gggttataca cctcaatatc ggatggagaa agcagttgca gaatactccg
      181 aatggattca taaacaactt cgttctaaaa aaacagttct agattataag taacaagtga
      241 aaatatggtg atgggtaaat agagatttac cctctccctt caaggatgga agtacaatta
      301 acatttttaa taaaattttc tccttggtct ttagcgagag aagttcatgt tgaatcttat
      361 taaccaaaaa attatttaaa aattttttag atataaaaat cgataaacca tgaaaaaggg
      421 agggaaaatc atttgaaaat aatcctatta gctgatctgg agggcgtttc tggagtagta
      481 gataacgagc agcaggctaa acctggagct cccctttacc aggaagcgag agaatatctg
      541 ttatccgacg tcaatgccgt cattgaagga gcgttagatg gaggagctac cgagataacc
      601 atctttgata tgcactacta tggtttaaat ctcaatctga gtaaaataca tcctcaaacc
      661 tgggttttta tgggaaaacc gcggaagatt tatcctcttt taaaagaagt atatcaaaag
      721 gccgatggtt ttatgatggt tggatttcat gctatggcac aaactccggg aggtttattg
      781 actcacactt atgaccattc tatcaagagt ctttacctca atggaattct gatgggagaa
      841 attggcatgg aggcagcaat tgctggtttt tatggagtac cgttaatttt tgttgctggt
      901 gattcgaaag ggacggaaga gaccgaaagc cttctgggtg acattcccac cgttacggtc
      961 aaatgggctg tcaacgagca cagtgcctta tgtcttccta ttgaagttac tcataatgag
     1021 atcaggaaaa ctgcgaaaga atcattggaa aaaatcacag aattcaaacc ttatgttctt
     1081 gaaccaccct atcatatcga agtagaattt ttccttcctg aaaaagccga aaaaatggag
     1141 aaactcgcag gcgttgcaag aatcgatgaa ttaaccgttc aagcccaggg agacgacctt
     1201 cctcttcttt gggaagaatt catcggcttc tgtcagaatt accaataagc aatttatttc
     1261 ctaaatgttg cacttgaagt cttcctatgc cctcgttttt ttgagggcat aaaggtttca
     1321 tctaacgttg cttatgtcat cctaaagcgt cgcttcccct tatttttttc accctctctc
     1381 cccctcgccc tcgtcctttt gttcgcgaga atctcattaa ccaaatccgt cattgcgagg
     1441 agaccaacct tttttggttg gacgacgtgg caatctcatc cggttcatct ttattatttt
     1501 tttactcgcc ccctcgcccc atctccccct cgccccctca gtacctaagg gcgtgaggat
     1561 ctcatctttt catttttttc cataatccca aaaccccgaa attcaagaat
//
LOCUS       DAOSOL010000330         1606 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_76865_length_1606_cov_2.981302, whole genome shotgun sequence.
ACCESSION   DAOSOL010000330 DAOSOL010000000
VERSION     DAOSOL010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76865_length_1606_cov_2.981302"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PK171_06725"
     CDS             <1..638
                     /locus_tag="PK171_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOT05915.1"
                     /translation="NQDWWMAPLTEWFCEKLGGKGNIVYISGIADQPGNIERDVSAEK
                     VLAKYPDIKLLAKANGNWDQAAAQQVMSDLLASFPQIDGVLTQDGMTLGIIRAFEAAG
                     REIPVVTGETQIAFIKKWKEMKDAVDFDTVGIVNPPGYVNNALGIGLRLLQGKKLKDE
                     VLVNGHIIYTKPNLIVDNNNIDEIYDQYKDWADSYYVNSWYSQEELDTLFE"
     gene            707..>1606
                     /locus_tag="PK171_06730"
     CDS             707..>1606
                     /locus_tag="PK171_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05916.1"
                     /translation="MSFILEARNLKKSFDGVVALSDANFTLNQGEICGLVGANGSGKT
                     TFARIISGLMHPDSGQLYLYGSQISLKSHLEAERLEISMVHQNLSLIPEMTVWENINL
                     GREFTTPLGILKKEAALNRAKEALQELQVNISFYDKVSQLAPSDKQLFEIVKALSRHP
                     KILILDEPTASLGFRQVDILFEKLNQLKNNGVSVIFISHRIWEITKICDRLVAFRNGK
                     TVGEVDFHQQPRDERLIIPLITGKKENEKSIHEKRSYRSFDTHQFVLEVDNLSKKEKL
                     HNVTFKIREGEIVGLGGLNGQGQE"
BASE COUNT          532 a          306 c          327 g          441 t
ORIGIN      
        1 tgaatcaaga ttggtggatg gcccctctta ctgaatggtt ttgtgaaaag cttggtggaa
       61 aagggaatat agtatacatc agtggaattg ccgatcaacc tggaaatatc gaaagagacg
      121 tttccgccga aaaagtactg gccaaatacc ctgatattaa acttttggcg aaagctaatg
      181 gtaactggga ccaggctgct gcacaacagg ttatgagtga tctcctggca tctttccccc
      241 aaatagacgg tgttcttacc caagatggaa tgactttagg aattattcga gctttcgaag
      301 ctgccggcag agagattcca gtggtcactg gtgaaactca aatagctttc attaaaaaat
      361 ggaaagagat gaaagacgct gttgattttg ataccgttgg aatcgttaat ccccccggtt
      421 atgtaaataa tgccctgggt ataggcttga ggcttttaca agggaaaaag ctgaaagacg
      481 aagtcttagt caatggtcac atcatttaca cgaaaccaaa tctaattgtc gataataata
      541 atattgatga gatttatgat caatataagg actgggcgga ttcctattac gtgaattcct
      601 ggtatagtca agaagaactt gatactctct ttgagtaaca atacatataa atttacgttc
      661 agggccagca aaacccagcc ctgaacgtaa atactggatc taaccaatgt ctttcattct
      721 tgaagcgaga aacctaaaaa aaagttttga tggagtagta gctctttctg acgctaattt
      781 taccctcaac caaggggaaa tttgtggcct agtcggagcg aatggttccg gtaaaactac
      841 ctttgcacga ataatcagtg gtctcatgca tcctgattca ggtcagctct acctttatgg
      901 atctcaaatt agcttaaaat cacaccttga agccgaaaga cttgagatct cgatggtcca
      961 tcagaacctc agcctcatac cggaaatgac ggtttgggaa aatatcaatt taggaagaga
     1021 gttcaccacc cccttaggaa ttctcaaaaa agaagcagct cttaatagag ccaaagaagc
     1081 tcttcaagaa cttcaggtaa atatttcttt ttatgacaaa gtatcccaat tggcaccttc
     1141 agacaaacaa ctctttgaaa tcgttaaagc tctatctcgt catccaaaaa ttctaattct
     1201 tgacgaacca actgcttcgc tggggtttag acaggttgat atattatttg aaaaactaaa
     1261 tcaattaaaa aacaacgggg tatcggtaat ctttatctcc catcggattt gggaaataac
     1321 caaaatctgc gatcgtttag ttgctttccg caatgggaaa accgttggtg aagttgactt
     1381 tcatcaacag ccaagagacg aaaggttgat cattcccctt attaccggaa aaaaagaaaa
     1441 tgaaaaaagc atccacgaaa aacgatctta tcgaagcttt gatactcatc aatttgttct
     1501 tgaagtggat aatctttcaa aaaaagaaaa actccataat gttactttta aaattcgaga
     1561 gggagaaatc gtcggattag gtggtttgaa tggccaaggt caagaa
//
LOCUS       DAOSOL010000331         1598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_77458_length_1598_cov_1.637071, whole genome shotgun sequence.
ACCESSION   DAOSOL010000331 DAOSOL010000000
VERSION     DAOSOL010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77458_length_1598_cov_1.637071"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PK171_06735"
     CDS             complement(<1..982)
                     /locus_tag="PK171_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOT05917.1"
                     /translation="MTNNMRVLVAGIHFESIIHFLRKNLPEFESYQIPASKVVEEVKE
                     ALVIIPTMTRIDRHIINSAPHLKLIQQWGSGLEGVDIQAATENGVAVANVPTHGTGNA
                     ESVAEWCIMSAIALSRSFPSIQESVWKGSPWGVPCGKSLMGKTAGIVGVGGIGVELAK
                     RLIPFQMKLIGVKKNPHHLPPGLSFEWVKGMNAFPELLNLADYVFLCLPQNEDTKCLF
                     NRKAFQLIKNGAYLINPARGGLIDREALFDALKGERLAGVAMDVFWQEPTNPQDPLLQ
                     NPKVLVTPHIAGATDTSYQGIAEGVVKNIRLTLTGKIPNNCVNPEVASHPK"
     gene            complement(999..1484)
                     /locus_tag="PK171_06740"
     CDS             complement(999..1484)
                     /locus_tag="PK171_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05918.1"
                     /translation="MQSITLIKNSEHLLPIDKQVSIPIIWPKEYERSIQILLKSCSNL
                     RPYLISIEPSDEERNILQEKIQKNDLKIIASYDLHRNTQWKELVHSLSNQKTILIALR
                     SPYDFLEVTDYGAAIATYGDRPVSIEALGKILKGEILPSGQLPVELPDRYQLGWGLKE
                     F"
BASE COUNT          469 a          342 c          284 g          503 t
ORIGIN      
        1 acttcgggtg agatgccact tctgggttta cgcaattatt tggaattttc cctgtcaagg
       61 ttaatcgaat atttttcaca acaccttcgg caattccctg ataggaagta tcggttgcac
      121 cggctatatg aggagtaact aatacttttg ggttttggag gagaggatct tgaggattgg
      181 taggttcctg ccaaaataca tccattgcca ctcctgctaa tcgttctcct ttaagagcat
      241 cgaaaagggc ttcacgatca atcaatcctc ctcgagctgg attaatcaga taagcgccat
      301 tcttgatcaa ctgaaaggct ttacgattaa aaaggcattt ggtgtcttca ttttgtggca
      361 agcataaaaa aacataatct gccaagttta atagctctgg aaaagcattc atccccttta
      421 cccattcaaa actcaaacct ggaggaagat gatgaggatt ttttttcaca ccgatcaatt
      481 tcatttgaaa cggaatcaat cttttagcta attcaactcc tattcctcca actccaacaa
      541 tcccagcagt ttttcccata aggctctttc cacaaggaac tccccatgga gaccccttcc
      601 aaaccgattc ttgtattgat gggaatgatc ggcttaaggc aatcgctgac atgatacacc
      661 attctgccac cgattcagca tttccagtac catgggttgg aacattagca acggctactc
      721 cattttcagt agcagcctgg atatccacgc cctccaatcc agaaccccat tgttgaatga
      781 gtttgagatg aggtgcacta ttgattatat gacgatcaat tcgagtcatg gttggtatta
      841 taaccaatgc ctcttttact tcctcaacga ctttcgaagc aggtatttga taggattcaa
      901 attctggtaa attcttcctt aaaaaatgga taatcgattc aaaatggata cctgctacca
      961 atactctcat attgttagtc attttctctt tttttccttt aaaactcttt caaaccccat
     1021 cccaactgat accgatcagg gagttccact gggagttggc cgcttggaag gatttcaccc
     1081 ttaagaattt ttcctaaggc ttcaatgcta accggccgat ctccgtaggt tgcaatagct
     1141 gctccataat cagtcacttc aagaaaatca taaggggagc gcagtgcaat caagatagtt
     1201 ttttggttgg ataaagaatg aaccagttct ttccattgag tatttcgatg aagatcataa
     1261 gaagcaatta tcttcaaatc attcttttgg atcttttctt gaaggatatt cctttcctca
     1321 tcagaaggtt caatagaaat caagtatggt ctcaaattgg aacagctctt taatagaatc
     1381 tgtatggatc tttcgtattc tttcggccat ataattggga tagatacctg cttatcaatt
     1441 ggaagaagat gttctgagtt ctttattaat gtaatacttt gcatggcaat ttctcgagcg
     1501 acatattatt gagaatttca tcaatatcaa caatatcttg acacagccca aaccaactca
     1561 aaagaaaaac aattattatg acatattcac tgaatttt
//
LOCUS       DAOSOL010000332         1595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_77605_length_1595_cov_3.922078, whole genome shotgun sequence.
ACCESSION   DAOSOL010000332 DAOSOL010000000
VERSION     DAOSOL010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77605_length_1595_cov_3.922078"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PK171_06745"
     CDS             <1..437
                     /locus_tag="PK171_06745"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05919.1"
                     /translation="VPRYLGVVGVELPGALDTLSVDRRRMRAQWEKYQKDLSEAFGCA
                     QRGLEENDLSLLGRAATLSSWIAQSFFPEPIYEKLIEICSSIGGLGVNRAHTGKAFGI
                     LFDTRVYPPKKMLQRVKEALRNEKADVFYNKVISGGARVMNL"
     gene            434..1030
                     /locus_tag="PK171_06750"
     CDS             434..1030
                     /locus_tag="PK171_06750"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT05920.1"
                     /translation="MKEIIFLRHGDTDATENHYFAGWSDLPLTKKGEKRIKAAKEILK
                     NYEYLEIWSSPLIRTRQTAQIVVNSNYSIQYTEDIKERSFGEWEGKNWELLEQEFPNQ
                     IKKWKESPLEFTPPLGESFADVLLRVKRFWQTLQDKNNGNYLVVTHAGVIRCLLVLLT
                     GMTFENSFHLLLNPGVIIKVRDDSGFFQVTGIINHEDL"
     gene            1027..>1595
                     /locus_tag="PK171_06755"
     CDS             1027..>1595
                     /locus_tag="PK171_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family cobalamin biosynthesis protein"
                     /protein_id="HOT05921.1"
                     /translation="MMLNAIRLICGFALDGLFGDPQGKWHPVVLIGKIINSLERLLFP
                     KKRNFKREFFMGFILVILMAGGMGILYYFLSRWLRQYLSLAFWALEIYLIFSFTAYRT
                     LIKRVNNVKLALEQDNIEGARQRLKHLVGRDTEHFSEKEIVRAGIESLAESFSDGILA
                     PLFYTALFGALGGLIYKISNTLDSMLGYK"
BASE COUNT          487 a          258 c          379 g          471 t
ORIGIN      
        1 cagttcctag atatttggga gtagtaggcg tagaattgcc cggtgctctc gatactctct
       61 cggtggaccg taggagaatg agagctcaat gggaaaagta ccaaaaggat ctctcagagg
      121 cttttggttg tgctcagcgg ggtctagagg aaaacgattt atcccttttg gggagagcag
      181 cgactctgag cagttggatt gctcagtcat tttttccaga acctatttat gaaaaactaa
      241 ttgaaatctg ttcttctatc ggtggattgg gcgtaaaccg agcccatacg ggaaaagctt
      301 ttgggatact ttttgatact cgggtatatc ctccgaagaa aatgcttcaa cgagtaaagg
      361 aagccttgag aaatgaaaaa gcagacgttt tttataataa agtcatttcc ggaggagcaa
      421 gggtgatgaa tttatgaagg aaattatttt tttaagacat ggagataccg atgcgactga
      481 gaaccattac ttcgctggtt ggagcgacct cccacttacc aaaaaaggtg agaaaaggat
      541 aaaagcggca aaagaaattc taaaaaatta tgagtatttg gaaatttggt caagccccct
      601 catccggaca cgacagacag ctcagatagt tgtaaattct aattattcga ttcaatacac
      661 cgaggacatt aaagaaagat cttttggcga gtgggaggga aagaactggg aactacttga
      721 gcaagaattt cccaatcaaa tcaaaaagtg gaaggaaagc cccttggaat ttaccccccc
      781 actgggagaa agctttgcag atgttttgct tagagtaaaa aggttttggc aaacacttca
      841 agataaaaac aacggtaact atttagttgt aacccatgct ggtgttattc gatgtttgtt
      901 ggttcttcta accgggatga cttttgaaaa tagttttcat ttattactga accccggagt
      961 gataataaaa gttcgtgatg attcgggatt tttccaagta acggggatta ttaaccatga
     1021 ggatttatga tgctgaatgc aattcgatta atatgcggct ttgctcttga tggattattt
     1081 ggagatcctc agggaaaatg gcatccagta gttcttatcg gcaagataat caatagcctg
     1141 gaaaggttgc tttttccgaa gaaaagaaac tttaaacgtg aattttttat gggttttata
     1201 ttggttattc tcatggctgg agggatgggg atactgtatt attttcttag ccgatggttg
     1261 aggcaatacc tttcgttagc cttctgggcg ttagaaatat atttaatttt tagctttaca
     1321 gcatatcgga cattaataaa aagagtaaac aatgtgaagc tagcattgga gcaagacaat
     1381 attgagggag ctcgtcagag attaaagcac ttggtgggga gagataccga acatttttcc
     1441 gagaaagaaa tagttcgtgc cgggatagaa tcgcttgccg aaagctttag tgacggaatt
     1501 ctagctccgc tattttacac cgctcttttt ggtgctttgg ggggtttaat ttataaaatt
     1561 tctaatactt tggattcaat gctgggatat aaaaa
//
LOCUS       DAOSOL010000333         1585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_78346_length_1585_cov_2.783660, whole genome shotgun sequence.
ACCESSION   DAOSOL010000333 DAOSOL010000000
VERSION     DAOSOL010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78346_length_1585_cov_2.783660"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="PK171_06760"
     CDS             <1..421
                     /locus_tag="PK171_06760"
                     /inference="COORDINATES: protein motif:HMM:NF036518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05922.1"
                     /translation="IYDYPQNSFVGWFLGNPGMNFIEGVLWDASQGKLQVGHLQLEFN
                     TSKNGGKDHKEVILGIRPENIEVSVSPKQGFFEAQSDFIEPIGNRIIIHLVAMKLKMK
                     AKIEKIKEINKGSKVWVKFPSERILLFDPKNGDKIFP"
     gene            448..1488
                     /locus_tag="PK171_06765"
     CDS             448..1488
                     /locus_tag="PK171_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOT05923.1"
                     /translation="MKAAFLISPKNIEIRETPDPKIVKDNQVKVKVRSVGICGSDAHF
                     FWDGRLAGWVVEEPLILGHESAGDVVEVGKGVKKFKVGDRVAVEPGIPCRQCEWCKRG
                     EYHLCPDMVFYAVPGVHGAFADYVMSEEDFVFPLPQSVSYEEGALMEPLSVAVEAMKL
                     GNLQLGDSVAILGAGTIGLLCAQAALVGGATEIFVSDVNPMKLEFVKRYSKNRITTID
                     AGKTNVLEEIMKLTKNRGVDKTFETAGTFETFHLAPMITRRGGRVTLVGIPPFKEYPY
                     RASDLFDRTVTLNAVYRYANDYPISIRLVEKKLVDLKSIVTHRFSLEEIQKGLEVVAT
                     RSDNVIKAVMNI"
BASE COUNT          494 a          241 c          380 g          470 t
ORIGIN      
        1 catatatgat tatcctcaaa acagttttgt aggatggttt ttaggaaatc ctggaatgaa
       61 ttttatcgaa ggtgttctgt gggatgcatc ccaaggaaaa cttcaagtag ggcatcttca
      121 attagaattt aatacttcaa agaatggggg taaagatcat aaagaggtaa ttcttggaat
      181 tcgtcccgag aacatagaag taagtgtttc cccaaaacaa ggtttttttg aagctcaaag
      241 tgattttatt gagccaatcg gaaatcgtat tattatccat cttgtggcta tgaaacttaa
      301 aatgaaagct aaaatagaaa agattaagga aattaataaa ggatccaagg tttgggtgaa
      361 atttccatct gagcgtattt tgctttttga tccaaaaaat ggagataaaa ttttcccata
      421 aactaataat agcgaggaga ataaactatg aaagctgcat ttttaatcag tccaaaaaac
      481 attgagattc gagaaactcc tgatccaaag atcgtaaaag ataatcaggt caaagtcaaa
      541 gtgagaagtg ttggaatttg tggatccgat gcccattttt tttgggatgg tcgattggcg
      601 ggatgggttg ttgaagagcc tctgatatta ggccatgaga gtgctggcga tgtggtggaa
      661 gttgggaaag gagtaaaaaa gtttaaagtt ggagatcgag tagctgttga accgggtatt
      721 ccctgcagac aatgtgaatg gtgcaagcga ggtgaatatc atctctgtcc ggatatggtt
      781 ttttatgctg ttccaggggt tcatggagcc tttgctgatt atgtgatgag tgaagaggat
      841 tttgtgtttc cccttcctca atcggtttcc tatgaagaag gggctctaat ggaaccactc
      901 tcggttgcag tggaggccat gaagctggga aacctccaac tcggtgattc ggttgccatc
      961 ttgggagccg ggacgattgg attattgtgt gcccaagcgg cattagttgg aggagcgact
     1021 gagatttttg tgagtgatgt caatccgatg aaattggaat ttgttaaaag atattcgaaa
     1081 aatcggatta cgacaattga tgcaggaaaa actaatgtct tagaagaaat tatgaagctc
     1141 accaagaatc gcggggttga taaaaccttt gagaccgctg gaacctttga aactttccat
     1201 cttgcgccta tgatcacccg gcgaggtggg agggtaactt tagtgggtat tcctcccttt
     1261 aaggaatatc cctaccgagc ttccgatctt tttgatcgaa cggtaactct gaatgcggtt
     1321 tatcggtatg cgaatgatta tccaatatcg attcgattag ttgaaaaaaa attggttgat
     1381 ctcaaatcaa tcgtcactca tcgattcagt ttagaggaga ttcaaaaagg tcttgaagtg
     1441 gtagctactc gaagtgacaa tgttataaaa gcagtaatga atatataaaa atcaatttta
     1501 aaccgagaaa aattagatat atcgataaac aaaggaggtt gctttacaat gttcaaattt
     1561 ggtgttgata cctttatctg gagtg
//
LOCUS       DAOSOL010000334         1582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_78537_length_1582_cov_5.079895, whole genome shotgun sequence.
ACCESSION   DAOSOL010000334 DAOSOL010000000
VERSION     DAOSOL010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78537_length_1582_cov_5.079895"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..793)
                     /locus_tag="PK171_06770"
     CDS             complement(248..793)
                     /locus_tag="PK171_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HOT05924.1"
                     /translation="MKVYIGDVKKEVGWTSKESLRKTFPPLSFGNEKTPFAQPVQVDL
                     IVTNCGSGLLLEGQIKTALILPCSRCLEPFIYKVEAPLKLEFRNLDRITRASDFEAEA
                     KSADDMYYYHEEDAVIDIEGGVIEALLVHFPMKPICSEGCQGLCPICGINLNQNHCDC
                     KKNNIDPRLAALKKIKLEKKV"
     gene            complement(796..>1582)
                     /locus_tag="PK171_06775"
     CDS             complement(796..>1582)
                     /locus_tag="PK171_06775"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOT05925.1"
                     /translation="KAPQIAVFDTAFHGTIPEFAYIYAIPYEYYEKYGIRRYGFHGTS
                     HRYVAGRAAKMMGKDLSELRLITCHMGSGVSFTAIKNGKSIDTSMGFTPLEGLVMGTR
                     TGDLDPYILLYLMEKENLSTKEMDQILNKKSGVVGISGLSSDTRDIEAAAPTNHRAQL
                     TLDIIAYRAKKYIGAYYAILGDLDGIVFTAGIGENSSYIRKTICEGLEPLGISIDNDK
                     NTVKRKEAFINSDQSKVKLMVIPTNEELMIALDTYQFVHDLER"
BASE COUNT          478 a          345 c          265 g          494 t
ORIGIN      
        1 agcaacctgc catggcatgt cgaatcttgg ttttcatccc catctgatgc tgagtgcaac
       61 accaggctta ccctgttata ttaaaaaatg aaaactcatt ataatagaat acccttccaa
      121 taatcaagag ctaccaacca ttggaaaaac attcaattta aataagggta ggttttgatt
      181 gccacatagt ataaaatact ttattctata ttgcaaacta tgggaaatgg ttgtgatact
      241 gattttctta aactttcttt tcaagtttaa ttttctttaa agctgccagg cgtggatcga
      301 tattattttt tttgcaatca caatgatttt gattgagatt tattccacag attggacata
      361 atccctgaca tccctcagaa caaatcggct tcataggaaa atgaactaat aaagcttcga
      421 taactccccc ttctatatca attacagcat cctcttcatg atagtaatac atatcatcag
      481 cacttttggc ttctgcttca aaatccgatg ctcgtgtaat ccggtcaaga ttacgaaatt
      541 caagcttaag aggagcttcc actttgtaga taaaaggctc aagacatcgg gagcaaggaa
      601 ggattaaagc tgttttaatt tgcccttcca gcaacaaccc cgaaccgcaa ttagttacta
      661 ttaaatctac ctgaaccggc tgagcaaagg gggttttttc gttaccaaaa gaaagcggtg
      721 gaaaagtttt tctgagactt tctttgcttg tccatcccac ctctttcttt acatcgccaa
      781 tataaacttt cattcttatc tctccaaatc atgaacaaac tgataggtat ccagggcaat
      841 cattaactcc tcattggttg ggataaccat taatttaacc ttggattgat cggagttgat
      901 gaaagcctct tttctcttta ccgtattttt atcattatca atagatatcc ctaaaggttc
      961 aagaccctca caaatggttt ttcttatata agacgagttt tctcctatgc cggctgtgaa
     1021 aactattcca tccaaatctc caagaatagc ataataggct ccaatatatt ttttggctcg
     1081 ataggctatg atatcgagcg tcagttgggc acgatgattg gttggagctg cagcttcaat
     1141 atcacgagta tcacttgaca atcccgatat tccaactaca ccactcttct tatttaaaat
     1201 ctgatccatt tccttcgtcg aaaggttctc tttctccatc aaatagagaa ggatgtaagg
     1261 atccaaatct ccagttcgcg ttcccataac taaaccttcc aaaggggtaa aacccatcga
     1321 agtatcaatt gatttcccat ttttaatggc ggtaaaacta accccgctgc ccatatgaca
     1381 ggttatcaaa cggagttcgg aaagatcttt gcccatcatc ttagctgccc ttcctgccac
     1441 atatcggtgc gaggtaccat gaaaaccgta acgacggatg ccatatttct cataatattc
     1501 atagggaatg gcatagatat aagcaaattc aggtatcgtt ccatgaaaag ccgtatcaaa
     1561 tactgctatc tgtggagctt tt
//
LOCUS       DAOSOL010000335         1581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_78595_length_1581_cov_3.896461, whole genome shotgun sequence.
ACCESSION   DAOSOL010000335 DAOSOL010000000
VERSION     DAOSOL010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78595_length_1581_cov_3.896461"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK171_06780"
     CDS             <1..263
                     /locus_tag="PK171_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05926.1"
                     /translation="NQAEVKVWERGAGPTLACGTGACAVLVAGVLNQKLSPKAEIKLP
                     GGSLTIEWSTENQHVYMEGPAQEVFNAELSEEVYESWMLHRE"
     gene            242..1399
                     /locus_tag="PK171_06785"
     CDS             242..1399
                     /locus_tag="PK171_06785"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: protein motif:HMM:NF006756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOT05927.1"
                     /translation="MDVASRVKNLPPYLFAEIEKKVENMRIQGKEVIDLGIGDPDLPT
                     PEFIIDRLCLEAKKAENHRYPSYRGLSAFRKAAASWYTKRFGVTLNPDREVVSLIGSK
                     EGIAHFAWCIVDPGDIVLASDPGYPVYKISTMLAGGIPLELPLLKKNQFLPDFTSFPE
                     SVLKRTKLIYINYPNNPTGATVDHKFFREVVDLARTYDFVIAHDLAYSEVSYDGYNAP
                     SIFEVDGAREVAVEFHSLSKTFNMTGWRVGFAVGNEKLIEALGRVKTNIDSGIFNAIQ
                     WSGVEALGRTQEVLDWILPIYTRRRNLVVESLQACGFEVEKPRASFYVWVPVPVGYTS
                     MSFAEHLLERTGVVVTPGTGFGQYGEGYIRISLTSPEEILTKAMEKIQNEL"
BASE COUNT          451 a          281 c          384 g          465 t
ORIGIN      
        1 ccaatcaagc cgaagtaaaa gtttgggaac gaggagcagg accgactttg gcctgcggta
       61 ccggagcatg tgcagtgttg gtagctggag tcctcaatca aaaattaagc cctaaagctg
      121 aaatcaagtt gcctggtggg agtttaacta ttgagtggtc aacagagaat cagcatgttt
      181 atatggaagg tccagctcag gaagtattta acgcagaatt gtcagaggag gtatatgagt
      241 catggatgtt gcatcgcgag taaaaaattt accaccttat ctctttgccg aaattgaaaa
      301 aaaggtggaa aacatgcgaa ttcaggggaa agaagtgatc gacttgggaa ttggtgaccc
      361 cgatcttcct actcccgaat ttattataga tcgcttatgt ttagaagcca aaaaagccga
      421 aaatcatcgt tatccttctt atcgtggttt atctgccttt cggaaagctg cggcgagctg
      481 gtatacaaaa cgctttgggg ttactttaaa tcccgatcgg gaagttgttt cgttaatcgg
      541 ttcaaaggaa gggattgctc actttgcatg gtgtattgtt gatcctggtg atatcgttct
      601 ggcgagtgat cctggctatc cggtttataa aatcagtacg atgttggccg gtgggattcc
      661 ccttgaactt cccctgctta aaaagaatca atttcttccc gattttactt cttttcctga
      721 aagtgtttta aaacgaacca aattgattta catcaattat cccaataatc caaccggggc
      781 tactgtagat cataaatttt ttcgggaagt agttgatcta gctcgtacct acgattttgt
      841 tattgcccac gacttggcct attctgaagt aagctatgat gggtataatg ctccctcaat
      901 tttcgaagtt gatggtgctc gtgaagtagc tgttgaattc cattctcttt ctaaaacctt
      961 taatatgact ggttggagag ttggttttgc agttggcaat gaaaagttaa tcgaggcttt
     1021 gggtcgggtg aaaaccaata tagattcggg aatttttaac gctatacagt ggtcgggtgt
     1081 ggaagctttg ggaagaactc aggaagtact ggattggatt ctcccaattt atacccgtcg
     1141 tcgtaatctg gtagttgaat ctttacaagc ttgtggattt gaagtcgaga aaccccgagc
     1201 cagtttttat gtatgggtac cggttccggt aggatatacg tcaatgagtt ttgctgaaca
     1261 tcttttagaa agaaccggtg tggtggtaac tccagggacc ggttttggtc aatatggaga
     1321 aggatatatt cgaatttcct taactagccc ggaagaaatt ttaacaaaag caatggaaaa
     1381 aattcaaaat gagttatgaa taaggggcaa atgaaaatga aaattattgt ccaaaaattt
     1441 ggtggaacat cggttcacac tcctgagtta cgtgaaaagg ctgcgttgaa gctaaaagaa
     1501 gcttatgaag agggatattc accagtggca gtagtctcag caatgggacg agcaggagct
     1561 ccctacgcta ctgatactct g
//
LOCUS       DAOSOL010000336         1560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_80094_length_1560_cov_2.950166, whole genome shotgun sequence.
ACCESSION   DAOSOL010000336 DAOSOL010000000
VERSION     DAOSOL010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80094_length_1560_cov_2.950166"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1560
                     /locus_tag="PK171_06790"
     CDS             <1..>1560
                     /locus_tag="PK171_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOT05928.1"
                     /translation="ICIILIILSCFSCSFALELKDFFYRADNSEIKLTFKFDNPVTWK
                     EVSPDPYRIALDIEGVANALSFSERPMNIGPLRNIMIRSSEPGIRIWCELSQPAQFEV
                     FDESNGRLLTILLKGPFSNSQLKENMFSFDFRDASVRDVLMALAKSAGVNIVVDDSVE
                     GTISLAFEGLSFDQALGYILKVRGLEQVKLGNNIIIGKKETLEENFDLLTSRRFTLKY
                     TDPDTAASVLSMYISEPNRISKDPATRSLLIRGREEELTKAEEVLKTIDVALETKIFP
                     LNNNLYEGKDQLDRIVQLIKIIVPDENRISLNYTLNENIAMSEEKIRVPVGNPYIVVQ
                     GTQEELEAVGELISNIDRKLPQIMIEAKIVEINRNKTKDLGVSWFVGDQEGQISFGEM
                     SLGGTMERQDLVSIRIQALEKKNLGRLVGNPRILTLSGKTAVISVGEKVPYRNIVMDS
                     EGNKSFPIQWLDVGVKMGVTPEVTQDGFIILHVFPKVSTFVEREYLLEGLTFKDPQPS
                     EKSADTTARLRA"
BASE COUNT          476 a          273 c          342 g          469 t
ORIGIN      
        1 gatttgcatc attctaatta tattatcctg cttttcatgt tcttttgccc ttgagctgaa
       61 ggattttttc tatcgtgccg ataattctga aatcaaacta acttttaaat ttgacaaccc
      121 agttacctgg aaagaagttt caccagatcc ttatcgtatc gctttggata ttgagggggt
      181 agcaaatgct ttatcttttt ctgaacgacc aatgaacatc ggtccattgc gaaatattat
      241 gattcgaagc tctgaaccag gtattcgaat ttggtgtgaa ctgtcccaac ctgctcagtt
      301 tgaagttttt gacgagtcaa atgggagact gctaaccatt cttttaaagg gacctttttc
      361 caattctcaa ctaaaagaaa atatgttttc ttttgatttc cgtgatgcca gcgttcgtga
      421 tgttttgatg gccttagcaa aatcagctgg agtcaacata gtcgttgacg attcggttga
      481 aggaactata tccctggctt ttgaagggct ttcttttgac caggctttag gatatatcct
      541 caaggtgagg ggcttggaac aggtaaaatt aggaaacaat ataataattg gtaagaaaga
      601 gactcttgag gagaattttg acttattaac atcacgaagg tttaccttaa aatataccga
      661 tcctgacact gctgcttccg ttctatcaat gtatattagt gagcccaacc gaatctccaa
      721 agatccagct acccgcagtc ttttaataag aggaagagaa gaagagctta caaaggccga
      781 agaagttttg aagactatag atgtggcttt ggagactaaa atttttccac tcaacaacaa
      841 tttatatgaa ggaaaagatc agctggatcg gattgtccag ttgattaaaa ttatcgttcc
      901 tgatgagaac agaatcagtt taaattatac tttaaatgaa aatatagcta tgagtgaaga
      961 aaagattagg gtaccagttg ggaatccata tattgttgtt caaggaactc aagaggagtt
     1021 ggaagcggtt ggtgagctca tctccaatat cgatcgcaaa cttcctcaga ttatgattga
     1081 agccaaaatt gtcgaaatca atcggaataa aaccaaggat ttgggagtca gttggtttgt
     1141 tggtgatcag gaaggacaga tatcttttgg tgagatgtct ttaggtggaa ctatggaacg
     1201 tcaagatttg gtgtcgatac ggatccaagc cttggaaaaa aagaatttag gccggttggt
     1261 ggggaatcct cgaattttaa ctttaagtgg caaaacagcc gttatcagcg ttggtgaaaa
     1321 agtcccctat cgtaatattg tgatggattc ggaaggaaac aaatcttttc caatacaatg
     1381 gctggatgtt ggagttaaaa tgggagtgac ccctgaggtc actcaggatg gatttattat
     1441 cctgcacgta ttccccaaag taagcacctt tgtagaaaga gaatacctct tggaaggttt
     1501 aacttttaag gaccctcaac ccagtgaaaa gtctgccgat accacagccc gattacgagc
//
LOCUS       DAOSOL010000337         1559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_80142_length_1559_cov_4.543218, whole genome shotgun sequence.
ACCESSION   DAOSOL010000337 DAOSOL010000000
VERSION     DAOSOL010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80142_length_1559_cov_4.543218"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PK171_06795"
     CDS             complement(<1..423)
                     /locus_tag="PK171_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOT05929.1"
                     /translation="MKPIQIATFNVNSVRSRLPILEQWLHKNPVELLALQETKVTDEF
                     FPVDFFHDLGYQIIYRGQKAYNGVAVATRFEPIRVHFGFNDGEDPLADTRLILIQTPH
                     FFLLNSYIPQGKSIDHLDYQFKMRFFQRIHNWFKNDFSL"
     gene            complement(450..>1559)
                     /locus_tag="PK171_06800"
     CDS             complement(450..>1559)
                     /locus_tag="PK171_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT05930.1"
                     /translation="KATKAAQVQSRIKMLEKFNLIEMEKEAKPVSIHFPPGERSGLEV
                     LKVENLSKKYDSRFVFKNINFSLYRGEKVALAGVNGAGKSTFTRIISGNEDPTSGQVI
                     YGDKVKIAFFSQESAQNLNYAHTIWEEISSIPTKMLDIEKRNLLGAFLFSGDDIYKPI
                     PVLSGGEKARLTLVKILMNETNFLILDEPTNHLDMSTNELFQRALMEYSGTVLIVSHD
                     RYFLDNLVERVIEIQDGKIYEYPGNYSYYNEKREQRQALSSGDSDQSEKEKTAQSKKR
                     EIRRIEAEARNFLSRKKREIQSRLLPIEDAISKLEKKKSENESLLCDPSILTDSEKVQ
                     TLMKELKEINEELAVFMQKWETIAEEMDQLNTKEE"
BASE COUNT          435 a          280 c          293 g          551 t
ORIGIN      
        1 aagagagaag tcatttttga accaattatg aattctttga aaaaatctca tcttaaattg
       61 gtagtcaaga tggtcaattg attttccttg aggaatatat gaattcaaca gaaagaagtg
      121 aggtgtttga attaggatca aacgggtatc ggcaaggggg tcctcaccat cattaaaacc
      181 aaaatgaacc ctgattggct caaagcgggt cgccactgca actccattgt aagctttttg
      241 tcctcgatag ataatttgat atccaaggtc gtgaaaaaag tcaaccggga agaattcatc
      301 ggttactttc gtctcttgta aggctaatag ctctaccggg tttttatgaa gccactgttc
      361 taatattgga aggcgggaac ggactgaatt tacattaaaa gttgcaattt ggattggttt
      421 cataaatcct cctttttaaa tttttttatt tattcttctt tggtattgag ttgatccatt
      481 tcttcggcta tggtttccca tttttgcata aagacggcga gttcttcgtt gatttctttg
      541 agctctttca ttaaagtttg aactttttct gaatcagtta atattgatgg atcacacaag
      601 agagattcat tttcagattt tttcttttca agttttgaga tggcatcttc aattggtaat
      661 aaacgacttt ggatttctct ttttttccgg gaaaggaagt ttctggcttc agcttcaatc
      721 cgtcgaattt cccgtttttt agattgagct gtcttttctt tttcagactg gtctgagtca
      781 ccagaagaaa gggcttgtcg ttgctctctt ttttcgttgt aataagaata attcccggga
      841 tattcgtaaa ttttgccgtc ctgaatttcg attacccgtt caactaaatt gtcaagaaaa
      901 tatcggtcat gggaaacgat gaggacagtt ccactgtatt ccattaaagc tctctgaaaa
      961 agttcattgg tactcatatc aagatggtta gtcggttcat caagaattaa aaaattggtt
     1021 tcattcatga ggatttttac taaagttaac cgagcttttt ctcccccaga taaaaccgga
     1081 atcggtttat atatatcgtc tccggaaaaa agaaaagctc ccaagaggtt tcttttttca
     1141 atatcaagca ttttagtagg aattgagctg atctcttccc aaatagtatg agcataatta
     1201 agattttgcg cactctcttg agaaaagaag gcaattttta ctttgtcacc ataaataact
     1261 tggcccgagg tgggatcttc attgccagat attattctgg tgaaggttga ttttccagct
     1321 ccattcactc cggcaagagc tactttttct cctcgataaa gagaaaaatt tatattcttg
     1381 aatacaaaac ggctatcata ttttttggat aaattctcga ctttgagaac ttccaaacca
     1441 ctcctttcgc cgggagggaa gtgtatagac accggtttag cttctttctc catttcaatc
     1501 agattgaact tttctaacat tttaattcga ctttgaactt gtgctgcttt ggtcgcttt
//
LOCUS       DAOSOL010000338         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_81364_length_1543_cov_3.084677, whole genome shotgun sequence.
ACCESSION   DAOSOL010000338 DAOSOL010000000
VERSION     DAOSOL010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81364_length_1543_cov_3.084677"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1202
                     /locus_tag="PK171_06805"
     CDS             249..1202
                     /locus_tag="PK171_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT05931.1"
                     /translation="MKKRNQSTRTINTKEFIVYIAFLAIFIFFSIILRGRGFLSSENL
                     MNIARQTAMISVMAVGMTFVLAAGEIDLSIGSIVALASLTTALALRHSSLLVGVVTGL
                     GTGVLIGMVNGLFVAKIGVPSFLVTLGMTGIITGLACWITQLKSIPVTNKTYNFIFGS
                     GDIGPISILFVWTVVLLIIGQVALRKTKFGRYVLATGGNKISALYSGINVERIKFSVM
                     VLNGALAALAGMLYAGRLHGARYTLGETDLMTVIAAVIIGGTSMSGGRGSVIGSIVGS
                     LIMGMINNGLILMGLSVDQQMIFRGLIIIIAVSLTMREKKK"
     gene            1216..>1543
                     /locus_tag="PK171_06810"
     CDS             1216..>1543
                     /locus_tag="PK171_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05932.1"
                     /translation="MSPSLPFLRIPKTAQLVVVTDLEQESFPVIHLKKLFPGRDFVVV
                     SPRDFKIGDWKKADKNFEGFIYLEEKENQLFPAKQPSENKIQLLEDGFQIQTVKWQEI
                     PLILLLG"
BASE COUNT          461 a          265 c          326 g          491 t
ORIGIN      
        1 gggcaaaatg agaagtctca gttagaaaac aattaaaggc acacccccct caatgggggg
       61 attcatttga tggtatttac agaatagaaa tttctattac cttatgaagg gagacaatga
      121 agcatttaca acagatacaa ataagttggg tttcttcata tttaatgaat attttatagg
      181 attttgattt attcagctcg ccaatagttt tccagaaaag aagaaaatct gaaagaagga
      241 gattgctctt gaaaaaaagg aaccaatcaa ctcgaaccat caataccaaa gaatttattg
      301 tgtatattgc ctttttagca atttttatct ttttttctat tatcctacga gggcgaggat
      361 ttctctcttc ggaaaacttg atgaacattg ctcgtcagac tgccatgatt tcggttatgg
      421 cagtagggat gacttttgtc ttagctgccg gagaaattga tctttcgatc ggttctattg
      481 tggcattagc atcactaaca actgcattag cactccgcca cagctcctta ttggttggag
      541 ttgttactgg gttgggtacc ggagttctaa ttggtatggt taacggtctt tttgtagcta
      601 aaatcggagt cccctccttt cttgtcacct tgggaatgac tggaattatt accggattag
      661 cctgctggat tactcaactt aaatcaattc cggtaactaa taaaacctat aattttattt
      721 ttggatcagg agatatcgga ccgatatcaa ttctttttgt ttggactgtt gttttgctca
      781 ttataggtca agttgcactg aggaagacca aatttggacg ttatgtatta gcaactggtg
      841 ggaataaaat atctgctctg tattctggaa tcaatgtgga aagaattaaa ttttccgtca
      901 tggttttgaa tggtgcctta gcagcgctgg ctggaatgct atatgctggt aggctacatg
      961 gtgcccgtta tactttaggt gagaccgatt tgatgacggt tatagcagca gttatcatcg
     1021 gaggcaccag catgtcagga ggccggggtt cagtaatcgg ttccattgtt ggttccctaa
     1081 taatgggtat gattaataat ggattgatat taatgggttt atccgtcgat caacagatga
     1141 tcttccgagg cttgatcatt atcattgcag tttctttaac catgagagaa aagaaaaaat
     1201 aaaggaggac aatagatgtc accatctttg ccatttttac gcataccaaa aactgctcaa
     1261 ctagtcgtgg taaccgatct tgaacaggaa agcttccctg ttattcattt aaaaaaatta
     1321 tttcccggaa gggattttgt tgtcgtttca ccacgagatt ttaaaattgg tgattggaaa
     1381 aaggcggata aaaacttcga aggttttatt tatttagaag aaaaggaaaa ccaacttttc
     1441 cctgcaaaac aaccgtcaga aaataaaatc cagctcctcg aagatggttt ccaaattcaa
     1501 acggtgaaat ggcaggaaat tccattgata ctccttttag gag
//
LOCUS       DAOSOL010000339         1542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_81431_length_1542_cov_3.897781, whole genome shotgun sequence.
ACCESSION   DAOSOL010000339 DAOSOL010000000
VERSION     DAOSOL010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81431_length_1542_cov_3.897781"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..>1542
                     /locus_tag="PK171_06815"
     CDS             420..>1542
                     /locus_tag="PK171_06815"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT05933.1"
                     /translation="MNLSAIHEAIEINRQSILDHFFELLRMPTISANPDHKDDLHRAA
                     QWLVQYCQNIGLQARILPTSGAPVVFAEHCPYPNLPTLLIYGHYDVQPVDPIEQWHTP
                     PFDPTIKGDMIYARGADDDKGQFFSFLKAMEIIYSKTGSLPGNYKIVIEGEEEIGSAH
                     FEELLSSQKELFRADSILIMDAGQFDRGVPALVYGLRGLAYFEITCAGPAFDVHSGQF
                     GGGVPNPATSLIQILSQLKNDQGQITIPGFYQDVHEPEPWEREQMAQLPFQEESIKKE
                     LGIRAFQQEMNYTPLESMTVRPTFDICGIWGGYTGSGSKTIIPARCSAKFSFRLVPNQ
                     TPSKIATLLLEHLKSIVPPDIDYRVEVLPGIEPILTNPHK"
BASE COUNT          424 a          377 c          279 g          462 t
ORIGIN      
        1 atgccgtcat cctaagcctt ttttcctttt cctttttctc cccctcaccc cctcgccccc
       61 tctccccctc ggcctttcaa aaagatgaga ttgccacgtc gtccaaccaa agtacagttg
      121 aactcctcgc aatgacggat ttggatggaa tgcgggcatg ataaatcaag cccttacaga
      181 agaatataaa aactgtaggg gcacaatata ttgttcccaa tctttattaa atgtttcgac
      241 ataccatagc acgtcgaatc gtaactatca ccttcatccc atttttttcc atatttattt
      301 tttctctcac ccctttcccc tcggtcttct taaaaataca aatccatgtc ttttcacact
      361 gaatatctgt tacaataaaa acatcactgg cacacttatc aactgaaagg ggaaaaaaaa
      421 tgaacttatc agcaatccat gaagctattg aaataaatcg tcagtctatt ctcgatcatt
      481 tttttgaact tctccgtatg ccaactatca gcgccaatcc cgaccataaa gatgatcttc
      541 atcgtgccgc acaatggttg gttcaatatt gtcagaatat cgggttgcag gctcgaatcc
      601 ttcccacttc aggagcaccg gtagtttttg ccgaacattg cccctatcca aatttaccta
      661 ccctgctcat ttatggtcat tatgatgttc agccagtaga tcccatagaa cagtggcaca
      721 cccccccatt tgatcccact attaaaggag atatgatcta tgctcgtggt gccgatgatg
      781 acaagggaca gtttttttcc ttcttaaaag ctatggaaat catttattca aaaaccggct
      841 cacttcctgg taattataaa attgttattg agggagaaga agaaattggg agcgctcatt
      901 ttgaggagct actatcttct cagaaagaac tcttccgggc tgactcgatt cttattatgg
      961 atgctggtca atttgatcga ggagttccag ctttggtcta tggtttaaga ggtttagctt
     1021 actttgaaat cacttgcgct ggaccagctt ttgatgtaca ttctgggcaa tttggcggtg
     1081 gagttcctaa cccggccacc tctctcattc agattctcag tcagctcaaa aatgatcagg
     1141 gacaaatcac cattccaggt ttttaccaag atgtccatga accagaacct tgggaacgag
     1201 agcaaatggc acaacttcct tttcaggaag aaagcataaa aaaagagctg ggtattcgtg
     1261 cttttcagca ggaaatgaac tacactcccc tggaaagtat gaccgttcga cctacttttg
     1321 acatctgtgg aatatgggga gggtataccg gatctggctc aaaaaccatt attcctgctc
     1381 gctgtagcgc aaaatttagt tttcgtctgg ttcctaatca aaccccttcg aagatcgcta
     1441 ctcttctttt agaacatctc aaatccatag ttcctcccga tatcgattat cgtgtcgagg
     1501 tgcttcctgg aatcgaaccg attttaacta atcctcacaa gc
//
LOCUS       DAOSOL010000340         1533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_82081_length_1533_cov_3.671177, whole genome shotgun sequence.
ACCESSION   DAOSOL010000340 DAOSOL010000000
VERSION     DAOSOL010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82081_length_1533_cov_3.671177"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PK171_06820"
     CDS             complement(<1..534)
                     /locus_tag="PK171_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT05934.1"
                     /translation="MDKKTIRDIPKDQLKGKKVLVRVDFNVPLNEKREVTDDTRIVMS
                     LPTIRFLLEQGARVILVSHLGRPKCGPKDELKMNPVALKLEQILGYKVQKADDCIGES
                     VAQMANKLKDGEVLLLENVRFYPQEEANDHEFARSLASLADIYVNDAFGTAHRAHAST
                     AGVAAFLPAYAGFLMEKE"
     gene            complement(551..985)
                     /locus_tag="PK171_06825"
     CDS             complement(551..985)
                     /locus_tag="PK171_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT05935.1"
                     /translation="MFIEELMIPEAPTVNDFDTVGDTIQFLNRRKLSGVPVVDLEQNL
                     VGYFSGSDFFNYIESEVKKELGSAALSFYEFRKKMRELFDKEVSEFLSEKSESLEVNQ
                     TEEDAIHFFLQSDFESVPVVRMGKVVGMLSKTKLLQVMLENK"
     gene            complement(1019..>1533)
                     /locus_tag="PK171_06830"
     CDS             complement(1019..>1533)
                     /locus_tag="PK171_06830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOT05936.1"
                     /translation="LNTSFISPWLTLISSIFLHGNILHLGSNMLYLWIFGNNVEDFLG
                     HFHYLIFYLISGIFAGLVHALLFPSSTIPTIGASGAIAGVMGGYLILFPGARVIVMLI
                     FGFFVTFVRVKAVLVLGIWILLQVVSGLTSLGMSSRGGVAWFAHIGGFLLGMLWCKIV
                     SNRYYRTHYW"
BASE COUNT          505 a          355 c          247 g          426 t
ORIGIN      
        1 ttctttttcc atgaggaatc ctgcataagc aggcaaaaaa gcagcaacgc cagccgttga
       61 tgcgtgggct cggtgggcgg tgccaaaggc atcgttgaca taaatatcag ctaagcttgc
      121 taatgagcgt gcaaattcat gatcgttcgc ctcttcctgg ggatagaatc gaacattttc
      181 cagtagaaga acctcaccat ccttcagttt attagccatt tgagcaactg attcgccaat
      241 acaatcgtca gctttttgca ctttataacc aagaatctgt tccagtttta aagcaaccgg
      301 attcattttc agttcatcct tcggtccaca cttcggtctt cccaaatgtg aaaccaatat
      361 aacccgagct ccttgttcca acaaaaaacg aatagttggc aagctcatga cgattcgcgt
      421 atcatcagtc acttcacgct tctcattcaa aggaacatta aaatcaaccc gtactaatac
      481 tttcttgcct ttcaactggt ctttaggaat atctcgaatg gtttttttgt ccaatttcta
      541 caacctcctc ttatttattt tcaagcataa cttggagtaa tttcgtctta ctaagcattc
      601 caaccacttt acccattcgt accaccggga ctgattcaaa gtctgattgg aggaaaaaat
      661 gaattgcgtc ttcttcagtt tggtttactt caagagattc acttttttca gataaaaatt
      721 cagaaacctc tttatcaaaa agttccctca tttttttcct gaattcgtaa aaagataagg
      781 cggcacttcc taattctttt ttaacttcgc tctcaatata attaaaaaaa tcgcttccag
      841 aaaagtaacc aactaagttt tgttccagat ctactaccgg aactccggaa agttttctgc
      901 gatttaaaaa ctgaattgta tccccaacgg tatcaaagtc attgacagtt ggagcttctg
      961 gaatcattag ttcttcaata aacaagtgcg ttcctcctct ggcatttctt aacaaacttt
     1021 accaataatg agttcgataa tatcggttcg agactatttt acaccataac atacctaaca
     1081 aaaaaccccc tatatgagca aaccaagcaa ctcctcctct tgaactcatg cccaatgagg
     1141 tcaagccaga aaccacctgg agaagaatcc aaattcctaa gaccaaaact gctttcaccc
     1201 gcacaaaagt aacaaaaaaa ccaaaaatga gcatgacaat aactctcgct cccggaaaga
     1261 ggatgagata cccgcccatt acaccagcaa tggcaccact ggcaccgatt gttggtatag
     1321 ttgacgaagg aaacagaaga gcatgaacca acccggcaaa tataccacta ataagataaa
     1381 aaatgagata atgaaaatgt cctaaaaaat cctccacatt attaccaaaa atccacaaat
     1441 aaagcatatt tgaaccaagg tggaggatgt ttccatgcaa aaatatcgaa ctaatcaagg
     1501 ttaaccaggg cgaaataaaa gaggtgttta att
//
LOCUS       DAOSOL010000341         1530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_82351_length_1530_cov_2.407458, whole genome shotgun sequence.
ACCESSION   DAOSOL010000341 DAOSOL010000000
VERSION     DAOSOL010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82351_length_1530_cov_2.407458"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..>1530
                     /locus_tag="PK171_06835"
     CDS             376..>1530
                     /locus_tag="PK171_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT05937.1"
                     /translation="MIMTITERIREMSQEYGKKIAVSMGDWFINYNELVVEIDKEAQK
                     LKKAGVKEGMRVVIVLPNHWHTLRLILGCFQIGAIPLLLSPKYPPKSVEKIFQNANAC
                     LLIAQTLHQELADRFPFAFIGENGEISFPDKINAAVDDLPSFDSPIAAFFSTSGTTGI
                     PKLVMLTHKNILSDIDSCFDLVDIYPEDRMLGVLPMFHVFGFSIAYILPLAKGMTLTI
                     VPSLYPANNLIESLKKYQSTVFLGVPAVFSILAGARAKVPFDLSPLRLPICGGDALPQ
                     RVREAFEKGFGLNIMEGYGITEASRVVSINPSPLTRVPGSAGPVIKAINLKIVDDNDK
                     ELPAGEIGEIILSGDPISPGYYNNPEENQISFRNQWFYTGDLGKIDEKGLL"
BASE COUNT          456 a          301 c          289 g          484 t
ORIGIN      
        1 gaattttact cgtcattgcg agacctcgtt ttttgaggtc gtggcaatct cttgaatcat
       61 cttttattat ttgtagaatt gtagaatcat agaattggaa aggatgagat tctcacgtcg
      121 tccggtcaaa gtcaccggac tcctcagaat gacagattgg gtggaagaga ttgtcagatt
      181 gccgtactca tcatactttt ttagatattt ttttatttag atatttttct atacccacaa
      241 ctctttaatt ccatagctat ttaacttttt atggtggcag aaataagaaa tatggaataa
      301 aatacataaa gaaatttatt ttactcagta tatatcagca aatgtttatt ctatacacgc
      361 tttgaggagg aaagtatgat aatgactata accgaaagaa ttcgagagat gagtcaggaa
      421 tatgggaaaa agatcgcagt atcgatggga gactggttta taaattataa tgaactcgta
      481 gttgagattg ataaagaagc tcaaaaactg aaaaaagctg gtgtaaaaga aggtatgcga
      541 gttgtcattg tgcttcctaa tcactggcat actttgcgtc tcatccttgg ttgtttccaa
      601 attggagcta ttcctctcct gctctcacca aaataccctc ccaaatcagt tgaaaaaatc
      661 ttccaaaatg ccaatgcttg tttactaata gcccaaacac tacatcaaga gcttgccgat
      721 cgattcccct ttgcttttat aggggaaaac ggagaaataa gtttccctga taaaatcaat
      781 gcagcggttg acgatttacc atcatttgat tcaccaattg ccgctttttt ttcaacttca
      841 gggacaaccg gaattcccaa attggtcatg cttactcata aaaatatact ctctgacatt
      901 gatagctgct ttgatttagt tgatatctat cctgaagatc gcatgttggg agttctaccg
      961 atgtttcatg tttttggttt ttcaattgct tatatccttc ccttagccaa agggatgaca
     1021 ttaaccatcg ttccttcgct ttatcctgcc aacaatctca tcgaatcgct caaaaaatac
     1081 cagtcaacag tttttttggg agttccagct gtcttttcaa tattagccgg agcccgtgct
     1141 aaagttccct ttgatttaag tcctctccgc cttcctatat gtggcggtga tgcgcttccc
     1201 caaagagttc gagaagcatt cgaaaaaggc tttggattaa atatcatgga gggatacgga
     1261 attaccgaag catctcgagt cgtttctatc aacccttctc ctctcacacg agtgccgggg
     1321 tcagcaggac cagtaatcaa agccatcaat ctcaagattg ttgatgacaa tgacaaagaa
     1381 ttgcccgcgg gtgaaattgg agaaattatt ctttctgggg atcccatctc accagggtat
     1441 tataataatc ctgaagaaaa tcaaatctct ttccgtaatc aatggtttta caccggcgac
     1501 ttaggaaaaa ttgatgaaaa aggtttactt
//
LOCUS       DAOSOL010000342         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_83165_length_1519_cov_3.848361, whole genome shotgun sequence.
ACCESSION   DAOSOL010000342 DAOSOL010000000
VERSION     DAOSOL010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83165_length_1519_cov_3.848361"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..863
                     /locus_tag="PK171_06840"
     CDS             21..863
                     /locus_tag="PK171_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05938.1"
                     /translation="MRLHNRQIKGSFWSDPDLIYELNRDQRMFFIGLTQLAEDSGCLE
                     YDPRSFKIILYPADNDINPEVLIEWTEKIIKMGKLIVYEVEGKKYLYIKNFPKHQSLR
                     TPAPPEIPLPIWICWKPREGSSRYGMYVYESSNNYVATPTYPCSNSNIPIKEENRIEE
                     NRIEENRIEENRIEEKKDICENLSAEAEPISPQDTSSSNHKKEIQEIMDFYNQQFSEC
                     WSSPLKLTKERYKHIRSRLKNFNIDDLKKAILNIRASPFHCGENDKGKVYATPEFLFG
                     MIPK"
     gene            912..1457
                     /locus_tag="PK171_06845"
     CDS             912..1457
                     /locus_tag="PK171_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05939.1"
                     /translation="MENQNMIGSFGNKRFCEYCGKEMNKYGEIMIDGQIKRINFLCDC
                     YIKAFDKEQEEEKRKARRLYLERFFANSEMGRRFENCSLLNFQVLQGTEESYEATKEF
                     IENFDDKIREGSGIVYLGTYGNGKTHLASAIYNHAKANEYPAIFHTMPTLMMRFNRTY
                     NDNDETEYDLVDLLIEADLLV"
BASE COUNT          566 a          239 c          312 g          402 t
ORIGIN      
        1 tggtgaggtg ggcgtagctt atgcgactcc ataaccgtca aataaaaggc agcttctgga
       61 gcgaccctga tttaatatac gaactcaatc gagaccagcg gatgtttttt attgggctta
      121 ctcaattagc tgaggactca ggttgtttgg aatatgatcc acgttcattc aaaataattc
      181 tttatccagc cgataacgat attaatccag aagtattaat tgaatggact gaaaaaatta
      241 tcaagatggg aaagttaatt gtttacgaag tagaaggaaa gaaatattta tatatcaaaa
      301 attttccaaa acatcaatca ctcagaacac cagctccgcc agagatacca cttcctatat
      361 ggatatgttg gaaaccaaga gagggaagtt caaggtatgg aatgtatgtt tatgaatcct
      421 caaataacta tgtagcaact cctacatacc catgtagcaa ttccaacata cctataaaag
      481 aagagaatag aatagaagag aatagaatag aagagaatag aatagaagag aatagaatag
      541 aagagaaaaa agatatatgt gaaaatttat cggctgaagc cgaaccaatt tctcctcaag
      601 atacttcttc atctaatcac aaaaaagaaa ttcaagaaat aatggatttt tataaccagc
      661 aattttctga gtgttggagt tctcctctta aattaaccaa agaaagatat aaacacattc
      721 gatccaggtt gaaaaacttc aacatagatg atcttaaaaa agctatttta aacattcgag
      781 cttctccctt tcattgcggt gaaaacgata aggggaaggt ttacgctacg ccagaatttc
      841 tatttggaat gattcccaag tagataagtg ggtgaaggaa ataaacatga aaggaggcac
      901 aattcaacca gatggaaaat caaaatatga ttggctcatt cggaaataaa aggttttgtg
      961 aatactgcgg aaaagaaatg aataaatacg gagagatcat gattgacggc caaataaagc
     1021 ggattaattt tttatgcgat tgttatatta aggcctttga taaggaacag gaagaagaaa
     1081 aacgaaaggc cagaaggctg tatttggaac gtttctttgc caactctgaa atggggagaa
     1141 ggtttgaaaa ttgcagtctt ctaaattttc aagttcttca gggaactgag gaatcatatg
     1201 aggcaaccaa ggaatttatc gagaattttg atgacaaaat aagagaagga tcagggatcg
     1261 tttatcttgg aacatatggc aacggaaaaa ctcatttagc ttcagcaatc tataaccatg
     1321 ccaaagccaa cgaatatcca gcaatatttc atactatgcc aaccctcatg atgagattta
     1381 accgaaccta taacgacaac gatgagacgg aatatgatct ggttgatcta ttgatcgaag
     1441 ctgatcttct ggtttagacg atatgggagc ggaaaagtgg agcgagaagg tacaggaaag
     1501 gttattcgct attttaagc
//
LOCUS       DAOSOL010000343         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_83187_length_1519_cov_2.907104, whole genome shotgun sequence.
ACCESSION   DAOSOL010000343 DAOSOL010000000
VERSION     DAOSOL010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83187_length_1519_cov_2.907104"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PK171_06850"
     CDS             <1..562
                     /locus_tag="PK171_06850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT05940.1"
                     /translation="EVQKMIHSIIASHHRIDILVNNAGLYAHGDALSFNEEIWEKIVS
                     VNIKGVLWCIKQVGLQMVKQKRGVIVNVASEAGLVGIANQIVYNLTKAAVISITQSCA
                     IDLIPYGIRTNCICPGTTLTPLVEASLAQASDPFSTREKLENSRPIQRLGKPEEIAAA
                     ILTLSSEVIGYAHGTIMNVDGGYTIW"
     gene            556..>1519
                     /locus_tag="PK171_06855"
     CDS             556..>1519
                     /locus_tag="PK171_06855"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT05941.1"
                     /translation="MVNLEPTPSLVIKNVSKKFPGVEALKNINLDIYSGEIHAILGEN
                     GAGKSTLMKILVGIIPRDSGEILLENKSLHLENPRLALQAGIALVHQDLSLLPYLSVA
                     ENIFLGRWPKKGISIDWESMIETTNQLLNRFQVKVDPRTLVGTLSVAEQQLIEILKAI
                     SHSHVKILLLDEPTSALSGEEINRLFQILLEIKSEGKSIIFISHKIDEAVKISDRITV
                     LKDGEKVITDTKLNLDEKTIIYHMTGKYIDLDNFRHTPPVKEKNIALSLEEVGTKFYK
                     KKISLNIYQGEILVVFGLMGSGKTTLARGLFGFETLEGTIRLFGN"
BASE COUNT          475 a          320 c          304 g          420 t
ORIGIN      
        1 agaagttcaa aagatgattc attccatcat cgcatcccat catcgaattg acatcttagt
       61 caacaatgcc gggttatatg ctcacggaga tgctttatcc ttcaatgaag aaatttggga
      121 gaaaatcgtc tcggtcaata ttaaaggggt cttatggtgt atcaagcagg tgggactgca
      181 aatggttaag caaaaacggg gagtaatcgt caatgttgct tccgaagcag gattggttgg
      241 aatcgccaat cagattgttt acaacctcac caaagccgcg gttatctcaa tcactcaaag
      301 ctgtgctatt gatttaatcc cttacggcat tcggaccaac tgcatttgtc cgggaacaac
      361 tctcaccccg ctggtagaag catccctggc acaagcgagc gatcccttct ctactcgtga
      421 aaaattagaa aacagccgac ctatccaacg gttgggaaaa ccagaagaaa tcgctgctgc
      481 tattctcacc ctcagctccg aagtaattgg ttatgctcat ggaaccatca tgaatgttga
      541 tggagggtac accatatggt gaatcttgaa cctaccccat ccctggttat caaaaatgtc
      601 agcaaaaaat tccctggtgt agaagcactg aaaaatatca atttagatat ttactctgga
      661 gagattcatg ccattttggg agaaaatggt gccgggaaaa gtaccctcat gaaaattctg
      721 gtgggcatta ttccccggga ttctggtgaa atattactgg aaaataaaag cctccattta
      781 gaaaaccctc ggcttgctct tcaagctggg attgccttgg tccatcaaga tttatccctc
      841 cttccctatc tctcggttgc tgaaaatatc tttttaggcc gatggcccaa aaaaggtatt
      901 agtatcgatt gggaaagtat gatcgaaacc accaaccaac ttctcaatcg atttcaagtg
      961 aaagttgacc cccgaactct ggtcggtact ctttcggtcg ctgaacaaca gttgattgaa
     1021 atcttaaaag ccatcagcca ttcccacgtt aaaattctcc tcctcgacga gccgacttca
     1081 gcactgagcg gagaggaaat caaccggctt tttcaaattt tattggaaat taaaagtgag
     1141 ggaaaatcga taatttttat ttctcataaa attgatgaag cagtgaaaat ctctgaccga
     1201 attacggttt taaaagatgg tgaaaaggta atcaccgata ccaagctaaa tctcgatgaa
     1261 aaaaccatta tttaccatat gaccggtaaa tatatagatt tagataactt ccgccatact
     1321 ccacccgtaa aagaaaaaaa cattgcttta tctctcgaag aggtaggaac taaattttat
     1381 aagaaaaaaa tcagtttaaa tatttatcag ggtgaaattc tagtcgtttt tggtttaatg
     1441 ggttcgggga aaaccacctt agctcgagga ctttttggtt ttgaaacctt agaaggaacc
     1501 atccgtttat tcggaaatc
//
LOCUS       DAOSOL010000344         1516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_83427_length_1516_cov_2.685147, whole genome shotgun sequence.
ACCESSION   DAOSOL010000344 DAOSOL010000000
VERSION     DAOSOL010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83427_length_1516_cov_2.685147"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1322
                     /locus_tag="PK171_06860"
     CDS             291..1322
                     /locus_tag="PK171_06860"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOT05942.1"
                     /translation="MKIVDLRRDTITLPTTAMVDTAFKATLGDSVYGEDPNQVQLEKL
                     AAEILGKEASIFVPSGTMGNLVALLSHTNRGDELICEENAHIRLSETGGGAMVGGLMF
                     RGIEDHSGVPDVSAIESAIRADDIHYPPTSLICTEITHYRYGGIIPPLEKIAAIYTFA
                     QKKGIPVHCDGARIFNAAVALNKEVSELTYYSDSVMVSLSKGLGAPVGSILVGQHDFI
                     EKAKKYRKMLGGGMRQTGWLAACGIVALQKENIQLLYEDHQNAIRLAQGIARLPGVKI
                     DFNQVQTNFILVDITQTSISGKLFLEKLKEKGILVTLAKPTLIRMVTSRVVNEQDIEY
                     TVETISNLL"
BASE COUNT          500 a          266 c          295 g          455 t
ORIGIN      
        1 agttttaaca tcaagtattt ggggatattg tatggatgag agtaaagaag gtaaaattat
       61 taccgaagat aaaaaagaaa tgtttcaaga gatatttaca gcttttcttc gagaatgaaa
      121 gaaggtttac caaaaagact taccatcctg ctatattata ttgcttgaac aaggaagtgc
      181 tcatccttaa gcagaatcaa tctctgtcca gagaaataag ttattttata ctatttaata
      241 ttcagattcc tattcatatc actcgctaat aagtaggatt gaggtgtaaa atgaaaattg
      301 tcgatttgag acgagacaca ataaccctcc caacgactgc tatggtcgat acagctttta
      361 aagccacctt gggtgattcg gtatatggtg aagaccccaa ccaagtccag ttagaaaaac
      421 ttgcggctga aatccttgga aaagaagcct caatatttgt cccaagtgga acaatgggaa
      481 acttagttgc acttctttcc cataccaacc gaggtgatga attgatttgc gaagagaatg
      541 ctcatattcg actatcagaa accggtggtg gagcaatggt agggggattg atgtttaggg
      601 gtattgaaga tcattctggt gttcccgatg tttcagccat cgaatcagca attcgagctg
      661 atgatattca ttacccacct accagcctta tttgtactga aattacccat taccgatacg
      721 gaggaattat tccaccatta gaaaaaattg cagccatata tacttttgcc caaaagaagg
      781 ggatacctgt tcattgcgac ggagctcgaa ttttcaatgc agcagttgct ttaaacaaag
      841 aagtaagtga attaacctat tattccgatt cggttatggt ttccttgtcc aaaggtcttg
      901 gtgctccggt cggatcgatt cttgttggtc aacatgattt tattgaaaaa gctaaaaaat
      961 atcgaaaaat gctaggtgga ggtatgcgtc aaactggttg gctggccgcc tgtggtattg
     1021 tggcgcttca aaaagaaaat attcaacttc tctatgaaga ccatcaaaat gcaattcggt
     1081 tagcacaagg gattgctcgt ctaccaggag taaaaatcga tttcaatcaa gtacagacca
     1141 atttcatttt agtcgatatc actcaaacta gtatttctgg caaacttttt cttgagaaat
     1201 taaaagaaaa aggaatcttg gtgactttag caaaaccaac cttaattcgc atggtaacct
     1261 cccgagttgt taacgaacaa gatattgaat atacagttga aactatcagc aatttattgt
     1321 aaaaatcatg atggtggtaa gtaagtccta ttgagttaaa acatcatttt ccacttcacc
     1381 atttctacaa gtgagaagga ctttttagtt gtcattacaa aaagagcaat taccagacct
     1441 tttctaaagt ttaatcttta tttttttcaa aaggaccaaa gggaaatata atatcaggat
     1501 acgaattctt gttctg
//
LOCUS       DAOSOL010000345         1512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_83733_length_1512_cov_3.397392, whole genome shotgun sequence.
ACCESSION   DAOSOL010000345 DAOSOL010000000
VERSION     DAOSOL010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83733_length_1512_cov_3.397392"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PK171_06865"
     CDS             <1..238
                     /locus_tag="PK171_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05943.1"
                     /translation="AGGHGFESRHLHQSLYRAIFFLSVFKPNLHKSPSPALLFSLPRN
                     QRGELFAGNCHNIPKFELPSLLKLVYFGKKAQGI"
     gene            266..568
                     /locus_tag="PK171_06870"
     CDS             266..568
                     /locus_tag="PK171_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05944.1"
                     /translation="MISLFFQWHPDAFINQKKLKKCVIQFFSWEVAPATFNIRRKYLK
                     VFFDYLTNEGVIEENPINFSARKEEGRTRSIPIDVIKKLLSAPDQKNFTGLRDLAF"
     gene            872..>1512
                     /locus_tag="PK171_06875"
     CDS             872..>1512
                     /locus_tag="PK171_06875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT05945.1"
                     /translation="MRKVGFIGLGNMGRGMCKNLILKGNQLTVFDINQDAMQSFKGKA
                     ALVHSPLEVLKNSEVIFLSLPNSIVVEKIINEFLTEGVQGKIIVDTSTSYPMSTRSLH
                     SKMKAAGGGLVDSPLIAGPAEADRGELIAVAAGDKEDVDKVDDLLRCYCKHYDYVGPS
                     GNGHLIKIAQNFAGLSQALIYAQLYPIIAKYGIHPQELYKSLNNEVFSNWVFQ"
BASE COUNT          463 a          297 c          289 g          463 t
ORIGIN      
        1 agccggaggc cacgggttcg aatcccgtca tctccaccaa tcgctttata gagcgatttt
       61 cttcttatcg gttttcaaac caaacctcca taaatctccg agtccggcgc tcttattttc
      121 gcttcctcgt aatcagcggg gtgagctatt tgccggaaat tgtcacaata taccgaaatt
      181 tgagctacca agtttactta aattggttta ttttggaaaa aaggcacaag gaatataagc
      241 acgaaccgaa atagattata gacaggtgat atcacttttt ttccaatggc atcccgatgc
      301 gttcatcaat cagaaaaagc ttaaaaaatg cgtcattcag ttcttctctt gggaggttgc
      361 cccagctact ttcaacatcc gtcgaaaata tctgaaggtt tttttcgatt atctcaccaa
      421 tgaaggtgtt atcgaggaaa atcccattaa cttttcggct aggaaagaag agggaagaac
      481 cagaagcatc ccaatagatg tcattaaaaa acttctcagc gctccggatc aaaaaaattt
      541 taccggttta cgagacctcg ctttttgagg ttgaagcaat agctttttga taatataatt
      601 atccccccag cggttctttc ctagatgatg ccctcatttt ttttaaatcg agaaattgta
      661 gaattggata ccaatagaaa taaaaaatta taataatgta ataaggattt taattcacct
      721 cttgggactc tccccgagaa tagctctact tcatgaaact ggatctaatt aaatatagtt
      781 tcacctttgt ctcctgtgac atataataac atgaggaata aaagcaatcg cttttgttgc
      841 gcctaattaa agaagggagg attttgatat catgagaaaa gttggattta ttggattagg
      901 caatatggga aggggtatgt gtaagaattt gattctgaaa ggtaaccaat taaccgtttt
      961 tgacatcaac caggatgcaa tgcaatcctt taaaggaaaa gctgcattgg ttcattctcc
     1021 tttggaagtt ttaaaaaata gtgaagtcat ttttctctca cttccaaatt ctattgtagt
     1081 tgagaaaatc atcaacgaat ttttaactga aggagttcaa gggaaaatca ttgttgatac
     1141 ttcgaccagt tatcctatgt cgactcgttc acttcacagc aaaatgaaag ccgctggagg
     1201 aggtttggtt gactctccgc tcatcgctgg ccctgccgaa gccgataggg gtgaattaat
     1261 tgctgtagca gctggtgaca aagaagatgt tgataaagtt gatgacctcc ttcgttgtta
     1321 ctgtaaacac tatgactatg taggaccatc gggaaacgga cacttaatca aaattgctca
     1381 aaatttcgcc ggactttctc aagcgctcat ttacgctcag ctctatccca ttattgccaa
     1441 atatggtatt catccacaag agctctataa atctctcaat aatgaagtat tttccaactg
     1501 ggttttccag tt
//
LOCUS       DAOSOL010000346         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_84304_length_1505_cov_2.443448, whole genome shotgun sequence.
ACCESSION   DAOSOL010000346 DAOSOL010000000
VERSION     DAOSOL010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84304_length_1505_cov_2.443448"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..>1505
                     /locus_tag="PK171_06880"
     CDS             271..>1505
                     /locus_tag="PK171_06880"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOT05946.1"
                     /translation="MKKLSHYSFFITCLFLIILSGTAIATESLYDIAYQTCVYAYPLV
                     LTKATQLSQNIPDNHFVHFRKLPSPSNRDIVRPNRDTMYSIAWLNLNKGPILLTVPET
                     IDRYYLVQIMDMWTDTFAGPSSRTTGSEGGRFLIVGPNWNGQLPEATSPEIKELINNF
                     RLIKSPTNQVWIMVRIEAGSEAEYPIVQKIQDGYQLEQIGIPKDQSSQILSNPRKDPR
                     FLKITQAIEKGAVTPPQVIEQMDAATFFEIFTELMIENPPHIQDWPIVALMSQIGIIP
                     GKALHFESLDENVQNALNQAVQDALKDIHVQTPIPFKDYWRFMPMFTGSYGANYMFRA
                     AIAFTGLGANLPEDAVYPVTNIDCNSEILDGSKNYVVHFEKDQIPPTNAFWSLTVYDS
                     EIYLIENPIHRYNLTSRQNV"
BASE COUNT          439 a          347 c          268 g          451 t
ORIGIN      
        1 tcttttattc acattttttt atcttttact caagataacc gggaaaaatc gaaagatccc
       61 ctccccactg tagagcgcca ctcctccaag gagaggaatt gttgaattca ttctcccatt
      121 gagggggatt caagggttgt gcccttctca tttttttaat cattttttac tcattcactt
      181 tatttccatg gtaattattt acaatgctta cattcaaatg tattatgaaa aaaatgaatt
      241 aattattttc ttaaaaaatg gaggggtaac atgaaaaaat tgagtcacta ttccttcttc
      301 attacctgtc tttttttgat tattctgagt ggtactgcta tagccaccga aagcctctat
      361 gacattgctt atcagacttg tgtttatgcc tatccgttag ttttaaccaa agcgactcaa
      421 ctcagccaga atatacccga taatcatttt gtccactttc ggaaactccc atcacccagt
      481 aatcgtgata tcgtccgacc gaatcgtgat accatgtatt caatagcttg gctcaacctt
      541 aacaaaggtc caatactttt aactgttcct gagaccatag accgttatta tctggtacaa
      601 ataatggata tgtggactga tacctttgcc ggccccagtt ctcgaaccac cggttcagaa
      661 ggcggccggt tccttattgt tggccccaac tggaatggcc agctccctga agcgacctct
      721 ccggaaatca aggagctcat caataatttc agattaataa aatcgcctac caatcaggtc
      781 tggatcatgg tcaggattga agctggtagc gaagcagagt atcccatcgt ccaaaaaatt
      841 caagatgggt atcagctcga gcaaattgga attccaaaag atcaatccag ccaaatccta
      901 tcaaacccac gaaaagatcc tcgttttctt aaaataaccc aagctattga aaagggagct
      961 gtaaccccac ctcaagtgat tgaacagatg gacgctgcta ctttttttga aatctttact
     1021 gaattgatga tcgaaaatcc tccccatatt caggattggc cgattgttgc cctcatgagt
     1081 cagataggaa taatcccggg taaggctctt cactttgaaa gtttagatga gaatgttcag
     1141 aatgctctta atcaagcagt tcaagatgct ttaaaagaca tccatgttca aacaccgatc
     1201 ccctttaagg attactggcg cttcatgcct atgtttactg gatcctacgg cgctaattat
     1261 atgttccgtg ctgctattgc ttttaccgga ctgggcgcga atcttcctga agacgccgta
     1321 tacccagtga ccaatatcga ttgtaactcc gaaatacttg atggaagcaa aaattatgtg
     1381 gttcacttcg aaaaagatca gatcccacct actaatgcct tttggtccct caccgtttat
     1441 gactcggaaa tatatctcat cgagaatcct atccatcgct ataatctaac cagcagacaa
     1501 aacgt
//
LOCUS       DAOSOL010000347         1505 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_84319_length_1505_cov_2.204138, whole genome shotgun sequence.
ACCESSION   DAOSOL010000347 DAOSOL010000000
VERSION     DAOSOL010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84319_length_1505_cov_2.204138"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>1505)
                     /gene="murC"
                     /locus_tag="PK171_06885"
     CDS             complement(98..>1505)
                     /gene="murC"
                     /locus_tag="PK171_06885"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT05947.1"
                     /translation="VSLPNELYFIGIGGTGMSALATIAFEMGYRVSGSDIFSGENTYR
                     LQEKGIPVFIGHSRERIENVPAVVISSAIPPENVELQAAIQRNIPIIHRGEMLAFFLN
                     QKKGIAIAGTHGKTTTTSMISLLLEVAGLDPTVLVGGEVEDIGGNARYGNGDYFVSEA
                     DESDGSFLKLNPYCGVITNIEDDHLEYYGSQENELNAFISFANGVKEGGFLVACGDHP
                     NVQMMFNQPLKTDLVSYGMVNDRVDIRGLIVEERAEGSLYRVTYHNCDFGSFILNIPG
                     IHNVINSLACIAVGIKLGIDRKLIATTLEMFRGVKRRYERIGYIDGTLVVDDYAHHPT
                     EMGVVLKTALSRTLGKVIVVFQPHRYTRTKRLYREMAEILKKAHQVILFPIYSAGEKP
                     IEGITSHLIYEEMKKTGYDGVHMVSSIDEALQITENMIGPGNILITMGAGDVWKVADS
                     MCRKNGNQLRKSIPILRT"
BASE COUNT          439 a          349 c          269 g          448 t
ORIGIN      
        1 tggagaatgg tgctttttaa aacatcaact aaagcgataa cccgaccacc aattttccag
       61 gttgtgtaaa atttcatttc agcttgtggt gtaataatca ggtccggaga atcggtatac
      121 tctttctcaa ttgatttcca tttttcctgc acatactgtc ggcaactttc catacatctc
      181 cggcccccat ggtaatcagg atatttcctg gacctatcat attttcagta atttggagag
      241 cttcgtcaat tgatgaaacc atatgaacac catcataacc ggttttcttc atctcttcat
      301 aaattaaatg agaggtaata ccttcgatcg gcttttcacc cgctgaataa atgggaaaga
      361 ggatcacttg atgggctttc ttcagtattt ctgccatttc tctataaaga cgcttggttc
      421 tggtgtaccg gtgaggttgg aaaacaacaa tgacttttcc taacgttcgg ctcaatgccg
      481 tttttaaaac aacacccatc tcggttggat gatgagcata atcatcaaca accagggttc
      541 catcaatata accaatcctt tcataacgcc tcttcactcc cctaaacatt tctaaggtcg
      601 tggcaattaa ttttctatca ataccaagtt tgattccaac cgctatacag gctaaggaat
      661 taatgacatt atgaatcccg ggaatattta atataaaaga tccaaaatca caattgtgat
      721 aggtgactcg atataatgaa ccttctgccc tttcttcgac tatcaacccg cgaatatcaa
      781 ctctatcatt gaccatgcca taagaaacca aatcggtttt tagaggctgg ttgaacatca
      841 tctgcacatt gggatgatct ccacaagcga ctaagaaacc gccttcttta acgccattcg
      901 caaatgaaat aaaggcattc aattcatttt cttgacttcc ataatattct aaatgatcat
      961 cttcgatatt ggtgatgacg ccacaataag gattgagctt taaaaaagat ccgtcactct
     1021 cgtcagcttc cgaaacaaaa taatccccat ttccataacg agcatttccc ccaatatctt
     1081 ctacttctcc acccaccaat acggtcgggt ctaggccagc gacttccaac aagagagaaa
     1141 tcattgaggt tgttgtggtt ttcccgtggg ttccggcaat tgcgatcccc tttttctgat
     1201 taaggaaaaa ggctaacatc tctccccgat ggataatggg gatatttcgc tgaatggccg
     1261 cttgaagttc gacattttct ggtgggattg cggaagaaat aaccaccgcg ggaacatttt
     1321 caattctttc ccttgagtga ccgataaata ccggaatccc tttttcttga agtcggtagg
     1381 tgttttctcc tgaaaaaata tcggaaccac taacccgata tcccatttca aaagcgatgg
     1441 ttgccaaggc actcatgcca gtgccaccaa ttccaataaa atagagttcg ttaggtaatg
     1501 agacg
//
LOCUS       DAOSOL010000348         1501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Atribacter sp. isolate AMR_MDS_2346
            NODE_84602_length_1501_cov_3.391425, whole genome shotgun sequence.
ACCESSION   DAOSOL010000348 DAOSOL010000000
VERSION     DAOSOL010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864413
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Atribacter sp. (wastewater metagenome)
  ORGANISM  Atribacter sp.
            Bacteria; Atribacterota; Atribacteria; Atribacterales;
            Atribacteraceae; Atribacter.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:14:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,380
            CDSs (total)                      :: 1,341
            Genes (coding)                    :: 1,334
            CDSs (with protein)               :: 1,334
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 36
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 3 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Atribacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84602_length_1501_cov_3.391425"
                     /isolate="AMR_MDS_2346"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2847780"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK171_06890"
     CDS             <1..196
                     /locus_tag="PK171_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05948.1"
                     /translation="PGTVEYEETLGVELIKALSGEKDAKQALDDAAQQWKEITDRLGY
                     EKQKEMFQALVRGWKEAGLW"
     gene            266..1147
                     /locus_tag="PK171_06895"
     CDS             266..1147
                     /locus_tag="PK171_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOT05949.1"
                     /translation="MFSLVKQVKLIGFFFVLPTIILLLVISIFPLIYSFWLSLCQWNI
                     GTGGERMFIGVHNYLNLLTDERFWNAMKNTGLELILGVGVQFLIGLGIALLLNRTFRG
                     KNLITTLYLLPMMISPVVVGCIWRIIYHYQYGPLNYIFNQVRIASVNWLGSGTVSIFS
                     IIIADIWEWTPFMMITLLAGLQAIPDELYEAARVDGATRWQTFSALVVPLLTPVMIVV
                     LLIRIMDAFKIFDLVVLLTMGGPGQSSETVSFFNYLTGFKYFNMGYASAMAYFQLLII
                     VIIANIFLKSWKRREMI"
     gene            1144..>1501
                     /locus_tag="PK171_06900"
     CDS             1144..>1501
                     /locus_tag="PK171_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT05950.1"
                     /translation="MNRIERRFNSFIILVIIVLLLVSLSPFIWNFMTSFKDKGEYFTY
                     PPVFISKTINFEHYTQAWNLGAGKGIQDSLIIAVFTTFIALVLGSLAAYALARFKIGG
                     ENLAFWILSIRMMPPVA"
BASE COUNT          379 a          263 c          328 g          531 t
ORIGIN      
        1 ccccggaacg gttgaatatg aagaaactct tggggttgaa ctgattaagg ccctttcagg
       61 agaaaaggat gctaagcaag ctctcgatga tgcggcccag cagtggaaag aaataaccga
      121 tcgattaggc tatgaaaaac agaaagagat gttccaagca ctggttagag ggtggaaaga
      181 agctggtctt tggtaatttg ttgaggattt gaattgaatt tgactaccgg gcgcttaacc
      241 cggtagtcaa attttttaga ggggggtgtt ttccttagtt aagcaggtaa aactaatcgg
      301 attctttttt gttcttccaa ctattattct gcttttagtg atctcaattt ttcctttgat
      361 ttattccttt tggttgagtc tctgccagtg gaacattggg actggtgggg aacggatgtt
      421 tatcggagtc cacaattatc ttaatctgct taccgatgag cgcttctgga atgccatgaa
      481 aaatactggt ttagagctta ttttgggggt tggagtacaa tttttaatcg gcttaggcat
      541 tgctctactc ttaaatcgca cctttcgagg taaaaattta attacaaccc tctatctcct
      601 tccaatgatg atttcaccag ttgttgttgg ttgcatctgg agaattattt accattatca
      661 gtacggtcct ttaaattata ttttcaacca ggttagaatc gcttcggtta attggttagg
      721 gagtggaaca gtttcaattt tttcgattat tattgccgac atatgggaat ggactccctt
      781 tatgatgatt accctgctcg ccggcttgca ggcaattcct gatgaacttt atgaagcagc
      841 ccgagtggat ggtgcgactc gttggcagac tttttcagca ttggtggttc ctctgctcac
      901 tccggttatg attgtagtcc tcctcattcg aataatggat gcttttaaaa ttttcgattt
      961 ggttgttttg ctgacaatgg gaggtcctgg tcaatcatca gaaaccgtat cgttttttaa
     1021 ttatttgacc ggttttaagt actttaatat gggttatgcc tcggccatgg catattttca
     1081 gttgctcatc atcgtcatca ttgccaatat ttttctcaaa tcttggaaga ggagggaaat
     1141 gatttgaatc ggatagaaag gaggtttaac agttttatta ttttagtcat cattgttcta
     1201 cttctagtat ctttaagtcc ttttatttgg aattttatga cttcatttaa ggataaaggc
     1261 gaatatttta cctatccccc agttttcatt tctaaaacta tcaattttga gcactacact
     1321 caagcttgga acttgggagc tggaaaagga atacaagaca gtttaatcat tgcggttttt
     1381 accacattca ttgcattagt tcttggttct ttagcggctt atgctttggc tcgttttaaa
     1441 ataggtggag aaaacctggc attttggatt ttgtctattc gtatgatgcc ccctgttgca
     1501 t
//
